LOCUS       DASUTW010000001         4299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_389233, whole genome shotgun sequence.
ACCESSION   DASUTW010000001 DASUTW010000000
VERSION     DASUTW010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389233"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VF058_00005"
     CDS             <1..383
                     /locus_tag="VF058_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206721.1"
                     /translation="EGVVYGSAEGLLLSVLPVVVTWQLLGALDWGDAWRGLAALAASV
                     VVIVAHHLGYPEYRGRGIVSPVIGCTALSVAYLATGSPIAAIGGHIVLHLAMQLRGME
                     LPPHAESILGGERAGGELAGARSG"
     gene            399..674
                     /locus_tag="VF058_00010"
     CDS             399..674
                     /locus_tag="VF058_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HEX6206722.1"
                     /translation="MVEIREDEEILREQAVERLKRKRDFTAHLLAYLMVNTVLIVVWA
                     MTGAGFFWPIFPLVGWGIGLGFHAWDTFSRPPSEERIQEEMDHLRAA"
     gene            676..1020
                     /locus_tag="VF058_00015"
     CDS             676..1020
                     /locus_tag="VF058_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206723.1"
                     /translation="MTDAVAVYAVAVGALMAVWWAVAWRGAWEREDRTRGELRLHLAA
                     ELLTAVALVASGIAVLAGGARAFLAAPLGMLLYTTVASPGYFLGRGELPVVAMFAVLA
                     ALTASGLVVLLV"
     gene            1072..2241
                     /locus_tag="VF058_00020"
     CDS             1072..2241
                     /locus_tag="VF058_00020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006128316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6206724.1"
                     /translation="MYDAIVVGARCAGSPLAMLLARRGHRVLLLERARFPSDTVSTHY
                     IHQKGVARLEAWGLLDRLRATGCPPILDMGWDVEGIRFVGRPPTPDGVTEGFGPRRLV
                     LDELLARAAEEAGAELREGFTVKEVVEEDGRAVGVRGRLRGGSMVEERARVVVGADGL
                     RSTVAREVGAERYEVHAPRTHAYYTYWEDVGLDRLDFYAVPGLGAAAFPTHDGLAMVS
                     IVFSTFAYPEGVRGRIEGSYLEGLRRFPALAERVLDGRRVERFAGMHRVPNHFRRSAG
                     PGWALVGDAGYHKDPAPAQGISDAFRDAEALAGAIDRGLREPPRVDDELEAYARGRDE
                     AALPWFRWALRMSRLEPLTPPLRQLYEAIAADQQWSNRFCGLSAEMVDPEAFFGR"
     gene            complement(2245..>4299)
                     /locus_tag="VF058_00025"
     CDS             complement(2245..>4299)
                     /locus_tag="VF058_00025"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX6206725.1"
                     /translation="HQTHFLERMDVLEVADDGATFLLNTPYGPDEVWERLPVEVQEQI
                     IVKGLRFFVVDGHRVAREAGLAGRINTVLQTCFFALSGILPREDAMAAIKKGIEKAYG
                     KRGREVLERNFAAVDAALEALHQVAVPDAIEGERHRLPPVPDEAPDFVRRVTGAIIAR
                     QGDLLPVSAFPVDGTFPSGTARWEKRSIALELPAWDPEICIDCAKCVLVCPHAAIRMK
                     VWDPEVVDAPAAVQHKAWRGKDFAGHRLTIQVAPDDCTGCAVCADICPAVSKEDPSHK
                     SLTMVPTADRLEADRAGWKAFQQLPTVDRTEVRPDTVKGSGLLEPLFEFSGACAGCGE
                     TPYLGLLSRLFGDRTLVANATGCSSIFGGNLPTTPWSKNDEGRGPAWSNSLFEDNAEF
                     GLGMRAGLDRRAERARRLLAGLSWELGWELPQAILRADQDSEEEIARQRARVAELKER
                     LEGMEPPEARELLGLADDLVRKNVWIVGGDGWAYDIGFGGLDHVLASGRDVNILVLDT
                     EVYSNTGGQASKATPLGAAAKFASAGKATAKKDLGMIAMTYGDVYVAQVALGADNPQT
                     VKALAEAEAYPGPSLVIAYGTCIEHGIEMATSMTHQKEAVAAGHWPLYRYDPRRVREG
                     SPAFQLDSRWKEGAFREFAMKETRFAALARSNPVRAEQLLEAAEEDIAERWRRYERLA
                     AG"
BASE COUNT          563 a         1516 c         1565 g          655 t
ORIGIN      
        1 gggagggggt cgtctacggg tcggcggagg gcctgctgct ctcggtgctg cccgtggtgg
       61 tcacgtggca gctgctcggg gcgctggact ggggcgacgc ctggaggggc ctggcggccc
      121 tggccgcaag cgtcgtggtc atcgtcgccc atcacctcgg ctaccccgag taccgggggc
      181 gggggatcgt ctcgccggtg atcggctgca ctgcgctgag cgtcgcctac ctggcgaccg
      241 gcagcccgat cgcggcgatc ggcggtcaca tcgtcctgca cctcgccatg cagctgaggg
      301 gtatggaact tccgccgcac gccgagagca tcctcggcgg agaacgagcc ggaggggagc
      361 tggcgggagc gagatccggc tgagaggagg atgaaccgat ggtggagata agagaggacg
      421 aggagatcct gcgggagcag gcagtggaga ggctgaagag gaagcgggac ttcaccgctc
      481 acctcctcgc ctacctgatg gtgaacaccg tgctcatcgt ggtctgggcg atgacgggtg
      541 ccgggttctt ctggcccatc ttccccctgg tcggttgggg gatcgggctc gggttccacg
      601 cctgggacac gttcagccgc cctcccagcg aggagcggat ccaggaggag atggatcacc
      661 tcagggccgc gtagcatgac ggacgcggtc gccgtctacg cggtggcggt aggagcgctg
      721 atggccgtgt ggtgggccgt ggcgtggcgc ggcgcgtggg agcgagagga tcggacccgc
      781 ggcgagctga ggcttcacct cgcggcggag ctcctgacgg ccgtagccct cgtcgcctcc
      841 ggcatcgccg tcctggcggg aggggcgagg gcgttcctcg ccgcccccct ggggatgctc
      901 ctctacacga cggtcgccag tcccggctac ttcctaggtc gcggcgagct ccccgtggtg
      961 gcgatgtttg ccgtcctcgc ggcgctcacc gcgtccgggc tggtggtcct gctggtgtga
     1021 ggggcagtgc tcggcttgat gcgggcgagc gctcgcccac atgatgtggg cgtgtacgac
     1081 gcgatcgtgg tcggagcacg ctgcgcgggc tcgcccctcg cgatgctcct ggcccgccga
     1141 ggccaccgcg tgctgctgct ggagcgtgcg cggttcccca gcgacaccgt ctcgacccac
     1201 tacatccacc agaagggcgt ggcgcgcctc gaggcgtggg gcctgctgga ccgcctccgc
     1261 gccaccggct gccctcccat cctcgacatg gggtgggacg tcgaggggat ccggttcgtc
     1321 ggccgtcccc cgacgcccga cggcgtgacg gaggggttcg gtccccgccg cctcgtgctg
     1381 gacgagctac tggcccgagc ggccgaggaa gccggcgctg agctgcgcga gggcttcacg
     1441 gtgaaggagg tcgtggagga ggacggccgg gccgtggggg tccggggaag gctccggggc
     1501 ggctcgatgg tcgaggagcg ggcccgcgtc gtcgtcgggg cggacggcct ccggtccacg
     1561 gtcgcccggg aggtcggggc cgagcgatac gaggtgcacg cccccaggac ccacgcctac
     1621 tacacgtact gggaggacgt ggggctcgac cgcctggact tctacgcggt gccgggcctc
     1681 ggagccgcag ccttccccac ccacgacggc ctggcgatgg tcagcatcgt cttctccacc
     1741 ttcgcctacc cggagggcgt gcgcggccgg atcgagggaa gctacctcga ggggctgcga
     1801 cggttccccg cgctcgcgga gcgcgtcctg gatgggagac gcgtggagcg cttcgccggc
     1861 atgcaccgcg tccccaacca cttccggcga tcggcgggcc cgggatgggc cctcgtcggg
     1921 gacgccggct accacaagga tccggccccc gcccagggga tctccgacgc gttccgcgac
     1981 gccgaggcgc tcgcgggggc gatcgaccgc ggcctgcggg agcccccccg cgtggatgac
     2041 gagctcgagg cctacgcgcg gggacgcgac gaggccgcgc tcccctggtt ccgatgggcg
     2101 ctccggatgt cgcgcctcga gcccctcacg cccccgctcc gccaactcta cgaggcgatc
     2161 gctgcggatc agcagtggtc gaaccggttc tgcgggctca gcgcggagat ggtcgacccg
     2221 gaggccttct tcggacgctg aggatcatcc ggcggcgagc cgctcgtagc gccgccaccg
     2281 ctcggcgatg tcctcctccg cggcctccag cagctgctcg gccctgaccg ggttcgaccg
     2341 agccagggcc gcgaaccgcg tctccttcat ggcgaactct ctgaacgcgc cctccttcca
     2401 ccgggagtcg agctggaagg cgggcgatcc ctcgcggacg cgccgagggt cgtaccggta
     2461 caggggccag tgacccgcgg ccacggcctc cttctggtgg gtcatgctgg tggccatctc
     2521 gatgccgtgc tcgatgcacg tgccgtaggc gatgaccagc gaggggccgg ggtacgcctc
     2581 ggcctcggcc agcgccttca cggtctgcgg gttgtcggcg cccagcgcca cctgagccac
     2641 gtacacgtcg ccgtaggtca tcgcgatcat cccgaggtcc ttcttggccg tcgccttgcc
     2701 cgccgaagcg aacttcgccg ccgcgccgag cggcgtggcc ttcgaggcct gcccgccggt
     2761 gttcgagtag acctcggtgt ccaggacgag gatgttcacg tcccgacccg acgccaggac
     2821 gtggtccagc ccgccgaacc cgatgtcgta ggcccatccg tcgccgccca cgatccacac
     2881 gttcttgcgc acgaggtcgt ctgccagccc cagcagctcc cgggcctccg gcggttccat
     2941 gccttcgagc cgctccttca gctccgccac gcgggcccgc tggcgggcga tctcctcttc
     3001 ggagtcctgg tcggcgcgga ggatcgcctg gggcagctcc catccgagct cccacgacag
     3061 cccggccagc agcctcctgg ctcgctccgc ccggcggtcg agcccggcgc gcatccccaa
     3121 gccgaactcc gcgttgtcct cgaacagcga gttggaccag gcggggccac gcccctcgtc
     3181 gttcttcgac cacggggtcg tggggaggtt gcccccgaag atcgaggagc agccggtggc
     3241 gttggcgacc agggtccggt cgccgaagag cctgctcagc aggccgaggt acggggtctc
     3301 cccgcagccg gcgcaggcgc cggagaactc gaacagcggc tccaggagcc ccgagccctt
     3361 caccgtgtcg ggtcgcacct cggtccggtc cacggtcggc agctgctgga acgccttcca
     3421 gccggcgcga tccgcctcca ggcggtcggc cgtggggacc atcgtcagcg acttgtggga
     3481 tggatcctcc ttgctgaccg cggggcagat atccgcgcag accgcgcacc cggtgcagtc
     3541 gtccggagcg acctggatcg tcagccggtg gcccgcgaag tccttccccc gccacgcctt
     3601 gtgctggacc gccgccgggg cgtccacgac ctcggggtcc cagaccttca tccggatcgc
     3661 ggcgtggggg cacacgagga cgcacttggc gcagtcgatg cagatctcgg ggtcccaggc
     3721 gggcagctcc agagcgatgc tgcgcttctc ccatctcgcc gtgcccgagg ggaacgtgcc
     3781 gtccacgggg aaggcgctga cgggcaggag gtcgccctgc cgggcgatga tcgctccggt
     3841 gacccggcgg acgaagtccg gggcctcgtc ggggaccggc ggcagccggt ggcgctcgcc
     3901 ctcgatcgca tcggggaccg caacctggtg gagggcctcg agcgccgcgt ccacggcggc
     3961 gaagttgcgc tccaggacct cccggccccg cttcccgtag gccttctcga tccccttctt
     4021 gatcgccgcc atggcatcct cccgcggcag gatgcccgac agcgcgaaga agcaggtctg
     4081 gaggacggtg ttgatgcgtc cggccagccc ggcctcgcgc gcgacccggt ggccgtccac
     4141 cacgaagaac cgcaggccct tgacgatgat ctgctcctgc acctcgaccg ggaggcgctc
     4201 ccagacctcg tccgggccgt acggggtgtt gaggaggaag gtggcgccgt cgtcggccac
     4261 ctcgagcacg tccatccgct ccaggaagtg ggtctggtg
//
LOCUS       DASUTW010000002         3657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_777997, whole genome shotgun sequence.
ACCESSION   DASUTW010000002 DASUTW010000000
VERSION     DASUTW010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777997"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..305)
                     /locus_tag="VF058_00030"
     CDS             complement(81..305)
                     /locus_tag="VF058_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206726.1"
                     /translation="MRHRRPPRALARLGAVATLAGAFVLFVSTLLHPLDSDPGVPRAA
                     FAEYGVDSLWEGSRDNSKVFLPTCVGRTRH"
     gene            complement(803..1129)
                     /locus_tag="VF058_00035"
     CDS             complement(803..1129)
                     /locus_tag="VF058_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206727.1"
                     /translation="MMRRLTALLGLAAVLTVAPMSLAGAITTAGGGTDAACELPGQLV
                     QADREVREACSARVASVELAPVLPEAESSGFPIGWTAGGGLVLLFGVGVLAIAAQRHR
                     RPRVLA"
     gene            complement(2016..2477)
                     /locus_tag="VF058_00040"
     CDS             complement(2016..2477)
                     /locus_tag="VF058_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206728.1"
                     /translation="MATIATQPQVEYRPGPRWMALFLVAAAGIGAGTVALVQTMTSEG
                     VTAGRTAAVSSVDGATQTRALNQPAAVAETSSSASFKLSAVLDLRSLNESEAPLSSFL
                     QGILDGRTLNEVRATAGSLATQAYRDRLTGLAEEIAAATKNLDGIADARTR"
     gene            2642..>3657
                     /locus_tag="VF058_00045"
     CDS             2642..>3657
                     /locus_tag="VF058_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6206729.1"
                     /translation="MATEASPFVGRVEELDRLTRGLDAAFQGRPGIVLIGGEAGIGKT
                     RLVRELSDRARERGARALSGGCVSLAEGTLPYLPVAEALRGLTTELGRPRLQSLLADR
                     PELAALVPDVPVRAAEPAGPTSASSRTRLFVELRALIGELAEETPLVLVLEDLHWADG
                     STLDLIAYLSRAVETERLLVLGTHRTDEPYPGHPLGAWLAESGRTERVETLRLPPLTV
                     EEIADHLAGLTGEAVAEDLVRRIHARSEGNPFFVEELATGSGERIPESLDALLLGRVG
                     TLSDPALEILRIGAVAGRRFDHGLVAEVAAVPADGLEPALREAVERRLLVTEGDGYAF
                     RHALL"
BASE COUNT          540 a         1270 c         1275 g          572 t
ORIGIN      
        1 ttcgaccagt tcgccgggat gcctgccctc gaccacatcg ctctggcggg cggtggcgcc
       61 gagtgatcgt gccgaacaga tcaatggcga gtccgtccaa cacatgtcgg gaggaagacc
      121 ttcgagttgt cgcggctccc ctcccacagc gagtccaccc cgtattcggc gaaggcggcc
      181 cgagggacgc ccggatcgct gtccagggga tggagcagcg tggacacgaa caggacgaac
      241 gctcccgcga gggttgcgac cgcccccagg cgggccagcg cccggggtgg tctccggtgc
      301 ctcatcccgc tcctgtcgag cgccggcctc agtgaccatc gtggcgctat catcagatcg
      361 gacgtgtggt gtcgtcgagg cgaggatctc gatgcgtcac acctcggttc gggttttcca
      421 ttccgtcgcg aacgttgggt tcccgcggtc gcgggcctcg acgtcggacg aggagtgagg
      481 cagtgcccgg ggggcaagca accccgcgga tcagggtcaa agatccccac accgcttggc
      541 gtcggtcagg acacggtgct tagtgcatgc acctcacgat ctcgatctgg aggtgctgcg
      601 gagctgtgga gcttctgctg gaggcattct gttggcgggt cgaagtatgc atctacgggc
      661 ctgaaggctt aggccagcgg ctacgctggc ccggctacta atcgacccgg aagtgtaccg
      721 cctacagagt cggagaaggc gggagccccg gccgagggag actcgaaacc ggggctccgc
      781 cgttccgtcc gagcggctgg ggtcaagcca ggacccgggg gcgccggtgc cgctgtgccg
      841 cgatcgccaa gacccccacg ccgaacagca ggacgaggcc tccgccggcc gtccacccga
      901 tggggaagcc ggacgattcg gcctccggca ggacgggcgc aagctcgacc gatgccaccc
      961 gggccgagca ggcctccctg acctcacgat cagcctggac gagctggccg ggcagctcgc
     1021 acgcggcatc cgtgcccccg ccggcggtgg tgatcgcccc cgccagtgac atcggcgcca
     1081 ccgtcagcac ggcggcaagt cccagtagcg ccgtgagcct tcgcatcatc gctctcctcg
     1141 ccataagtcc cccttctcac cggccgcgcc gatcgcgacc ctccacccga cggtaaggct
     1201 gaggggggcg ggcgcacatc cggcgggatc cctagatgtg aggtcggagg tccttattcg
     1261 tgtgcggcct ctcgccctcg tcccggttgg gcagagtggg cgtcatgacc accgaagcaa
     1321 ttcgagttcc tatgccggaa agagaaggag tttcattgag gctgcttgtg ccgcgactgc
     1381 gcttcatccg ccggaagctc tcctgccgct cgacactcca catgaacgcg cactactcga
     1441 cggtcaacga tcttctgcta tcgaagaacc ggtggccggc agcggcgcgc aggaaggcgc
     1501 ggagctcgtc gggggtccac ggagacggtt agaccctggg gatggaccgc aggcgcggcg
     1561 gtgggccacg agggccacgc gtggggagat cagcccctgg cgaacggcgt cgccgagcgc
     1621 gcaacggatg atcaggtgga tgtcggcaag acttaatagt acgcctgcgg taggagccgg
     1681 cgtccgtgag tgagtcaatc ggttgggtca tcactcccgg cacgcatcct gccgtcgaag
     1741 ccttccccct tccgccgagc cgctcgggta gtgagaaggg ttggcctctc ctccctccat
     1801 cgggcgtcgg aactcacagc tgagctgtgg cccctgctcg cctaccctct aggtctggac
     1861 caggcggact agcccagccc aaagggagga ggggaaagtc ccctcctccc tttgctcccc
     1921 ctcatccgag ggggagacga ccccgcgacc tcgtatccga ggccctcggc gtcctcgatc
     1981 acgccctagg acggattcgc tctccgcggg cccgttcagc gcgtcctggc gtcggcgatc
     2041 ccgtccaggt tcttcgtggc cgcggcgatc tcctcggcca ggcccgtcag gcggtcccta
     2101 taagcctggg tggccagcga cccggcagtc gccctcacct cgttgagcgt cctgccgtcg
     2161 aggatgccct gcaggaacga cgacagcggg gcctccgact cgttcaggct ccgcagatcg
     2221 agcaccgcgc tgagcttgaa cgacgcggag ctggacgttt cagctaccgc cgccggctgg
     2281 ttcagcgccc tcgtctgggt cgccccgtcg acggaggaga ccgcggccgt gcgtcccgcg
     2341 gtgactccct ccgaggtcat ggtctgcacc agggccacgg tccccgcccc gatccctgcg
     2401 gcggcgacca ggaacagcgc catccacctc ggacccgggc gatactcgac ctggggctgc
     2461 gttgcgatgg ttgccatctc tacctcccta tctctgcggc cgcgccgatc gcgaccgtcc
     2521 aacccgacgg tagggctgag gggggcgggc gcacatcggg cgggatccct agatgtgagg
     2581 tcggaggtcc ttattcgcgt gcggcctctc gcccttgtcc cgattgggcg agaatggcct
     2641 catggccacc gaagcctccc ccttcgtcgg cagggtcgag gagctcgatc gcctcactcg
     2701 agggctggac gcggcgttcc aggggcggcc gggcatcgtc ctcatcggag gagaggccgg
     2761 gatcgggaag acccggctgg tgcgggagct ctccgatcga gcccgggaac ggggggcgcg
     2821 agcgctgagc ggaggctgcg tgagcctggc tgagggcacg ctcccatacc tccccgtggc
     2881 ggaggcgctt aggggcctca ccaccgagct cggacgcccg cggctccagt cgctgctggc
     2941 cgaccggccc gagctggccg cgctcgtccc cgacgtaccc gtccgggccg ccgagccggc
     3001 cggcccgacc agcgcctcgt cccggacccg gctgttcgtc gagcttcggg ccctcatcgg
     3061 cgagctggcg gaggagaccc ccctggtcct cgtgctcgag gacctgcact gggcggacgg
     3121 gtcgaccctg gacctgatcg cgtatctctc ccgcgccgtg gagaccgagc gactgctggt
     3181 gctcgggacg catcggaccg acgagcccta cccaggccat ccactggggg cgtggctggc
     3241 tgaatcgggg cggacggagc gagtggagac gctccggctc ccgccgctca cggtcgagga
     3301 gatcgccgac cacctggccg ggctcaccgg ggaagccgtg gccgaggacc tggtccggcg
     3361 gatccacgcg cggtccgagg ggaatccctt cttcgtggag gagctggcca cggggagcgg
     3421 cgagaggatc cccgagtcgc tcgacgccct cctgctgggg cgggtgggca ccctctctga
     3481 ccccgcgctg gagatcctcc ggatcggggc cgtggccggg cggcgcttcg atcacggcct
     3541 ggtggccgag gtggcggcgg tccccgcgga cggcctggag cccgcgctcc gcgaggccgt
     3601 cgagcggcgg ctcttggtca cggaaggaga cgggtacgcg ttccgccacg ccctgct
//
LOCUS       DASUTW010000003         2238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_519438, whole genome shotgun sequence.
ACCESSION   DASUTW010000003 DASUTW010000000
VERSION     DASUTW010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519438"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1300)
                     /locus_tag="VF058_00050"
     CDS             complement(23..1300)
                     /locus_tag="VF058_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206730.1"
                     /translation="MKVRDVVATAVATILVLGLAGPASAAKPKTRLISRATSGTPGDN
                     DSYNPVLSASGRYIAFESYASNLPGGNGSNEQVYVHDRVTKKTRLVSKFAGQPGTGDS
                     YDPSISGDGRYVSFTSTASNFPGGDGATEQIYVHDRVTKKTRLVSKTPGGAPGGGHSS
                     DSAISGNGRVVVFETEAPNLGGSGADEQLRVYNLRKKKGRLLSKTKAGVPGDGTSNDP
                     AISHSGRYVVFETDAPNLGANNSPDDQIALWDRKTGRIRIVSRSSSGAKADGDSDDPN
                     ISANGRWVTFSADSTNLGPADGTYDQAYLRDLAKRKTRLVSKSSGGAIGDDDSGDGAV
                     SDDGRFIVFETFAPNLGANGDFQILLRDMKKNMTKLMSRDAAGAPGSWAFMSRGQRTI
                     TPDGRFVSFDARGTNLPGGAGGFNQTYIRGPLI"
     gene            1463..1544
                     /locus_tag="VF058_00055"
     tRNA            1463..1544
                     /locus_tag="VF058_00055"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1497..1499,aa:Tyr,seq:gta)
     gene            complement(1583..2071)
                     /locus_tag="VF058_00060"
     CDS             complement(1583..2071)
                     /locus_tag="VF058_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX6206731.1"
                     /translation="MRHRPLALAVCLLLAACGAEEPRRSDPSPSPEDLPFPTDATLVI
                     EPVQGDAVRLEVAIADTDEERTRGLMEVAEMPDDVGMVFLHEEPTESDFWMKDTLIPL
                     TVAVWDQSMTIRQVVDMQPCREDPCEIYDITLAWVGAVEVNQGALEAAGVGVGDTVRL
                     EG"
     gene            2099..2172
                     /locus_tag="VF058_00065"
     tRNA            2099..2172
                     /locus_tag="VF058_00065"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2133..2135,aa:Thr,seq:ggt)
BASE COUNT          306 a          776 c          766 g          390 t
ORIGIN      
        1 ggcgccggcg gcgccccccg ccctagatca gcgggcccct gatgtacgtc tggttgaagc
       61 cgcccgcgcc gccggggagg ttggtgcccc tggcgtcgaa ggacacgaag cgcccgtccg
      121 gggtgatggt ccgctggccc ctggacatga aggcccagct cccaggggcg ccggccgcgt
      181 ccctgctcat cagcttggtc atattcttct tcatgtcccg aagcaggatc tggaagtcgc
      241 cgttcgctcc caggttcggg gcgaacgtct cgaagacgat gaagcgcccg tcgtcgctga
      301 ccgctccgtc cccgctgtcg tcgtcgccga tcgccccccc gctcgatttg ctcacgaggc
      361 gggtcttccg cttggcgagg tcccggaggt acgcctgatc gtaggtcccg tcggccggcc
      421 ccaggttcgt ggagtccgcg ctgaaggtca cccaccgccc gttcgcggag atgttcgggt
      481 cgtccgagtc gccgtcggcc ttcgcgccgc tgctgctgcg actcacgatc cggatccggc
      541 cagtcttccg atcccagagc gcgatctgat cgtcggggct gttgttggct cccaggttgg
      601 gggcgtcggt ctcgaacacg acgtaccgac ccgagtgaga gatcgccggg tcgttgctcg
      661 tgccgtcgcc cggcaccccc gccttcgtct tgctgaggag gcggcccttc ttcttccgga
      721 ggttgtacac gcggagctgc tcgtcggccc cggatccccc caggttgggg gcctcggtct
      781 cgaacacgac gacccgcccg ttccccgaga tggccgagtc ggagctgtgg ccgccgcccg
      841 gcgctccgcc gggggtcttg ctgacgaggc gggtcttctt ggtgacgcgg tcgtgcacgt
      901 agatctgctc ggtcgccccg tcccctccgg ggaagttcga cgcggtggac gtgaacgaca
      961 cgtagcggcc gtccccggag atcgagggat cgtagctgtc gccggtgccc ggctgtcccg
     1021 cgaacttgct gaccagccgc gtcttcttgg tcacgcggtc gtgcacgtag acctgctcgt
     1081 tcgacccgtt cccgcccggg aggttggacg cgtaggactc gaaggcgatg taccggccgc
     1141 tcgcggacag caccgggttg tagctgtcgt tgtcgccggg cgttccggac gtggccctgc
     1201 tgatcagcct cgtcttcggc ttggcggcgg acgcgggtcc ggccagcccg aggacgagga
     1261 tcgtggcgac cgcggtcgcc acgacatcgc gaaccttcat cagctgcctc cttaggacgg
     1321 tgggcaagca cgacatgagc cgccccgaaa cgcttgcctc gtccgtgggg cagggcgcat
     1381 catacggccc cgctccgagc agagcaatgg cccaggagga cacggacgtg gtccctccca
     1441 tcggataccc ttgatcttgc gcggggggat gcccgagcgg ccaaagggag cggcctgtaa
     1501 agtcgccggc gtagcctacg gaggttcgaa tcctcctccc cccacgaacc ctggagggat
     1561 ccgccggcgc cctgaggtcg ggctagccct ccaggcggac ggtgtctccc acccccaccc
     1621 cggcggcctc gagcgctccc tggttcacct ccaccgctcc cacccatgcc agggtgatgt
     1681 cgtagatctc gcatgggtct tcccggcacg gctgcatgtc cacgacctgg cggatcgtca
     1741 tggactggtc ccagaccgcc acggtcagcg ggatcagggt gtctttcatc cagaagtcgc
     1801 tctcggtggg ctcctcgtgg aggaagacca tgccgacgtc gtcgggcatc tcggccacct
     1861 ccatgaggcc gcgcgtccgc tcctcatccg tgtccgcgat cgcgacctcg agcctcaccg
     1921 cgtcgccctg gaccggctcg atcacgagcg tggcgtcggt ggggaagggg aggtcctccg
     1981 gggagggaga ggggtcggac cgtctaggct cctcggcccc gcacgccgcc aggagcaggc
     2041 agacggcgag cgccagtggg cgatgtcgca tgtcagccac tatcatggca ccggttgcgc
     2101 tggagtagct cagctcggca gagcaccgcc atggtaaggc gggggtcgtg ggttcgagtc
     2161 ccacctccag ctcccggcac gagacggttg ctagactgag acgggcgcgg cggggtagct
     2221 cagaggcaga gcaagcgg
//
LOCUS       DASUTW010000004         9525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_260581, whole genome shotgun sequence.
ACCESSION   DASUTW010000004 DASUTW010000000
VERSION     DASUTW010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9525
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260581"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VF058_00070"
     CDS             complement(<1..502)
                     /locus_tag="VF058_00070"
                     /inference="COORDINATES: protein motif:HMM:NF015427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HEX6206732.1"
                     /translation="MAELEERLSDPSLWDDHARAQQLTTRLARLRDDVERYEGLAERL
                     EEAEVLDQLLDEQADPDTERELKEHLESLAADVDRLELAALLSGEFDDADAIASLHAG
                     AGGTESQDWAEMLLRMYLRWAEREGFEVELDEVSPGEEAGIKSATVTVKGKNAYGILS
                     AERGVHR"
     gene            683..1024
                     /locus_tag="VF058_00075"
     CDS             683..1024
                     /locus_tag="VF058_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX6206733.1"
                     /translation="MRRSSRSAARDRLHDALEALQRDGEPVILRDGRRPVAVLITVDD
                     YRKRFSRIHDESTPNGDGPTWPQVERREGDRRRGERRGRRRDPAPREGDPGGTLDVLR
                     ELRSLGLTAER"
     gene            complement(1038..3701)
                     /gene="secA"
                     /locus_tag="VF058_00080"
     CDS             complement(1038..3701)
                     /gene="secA"
                     /locus_tag="VF058_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX6206734.1"
                     /translation="MVFSKVQQIGEKREQKRLSHWVELVSSYEPETEDLTDDQLRGKT
                     DELRARLKEGETLDDLLPEAFAAVREAARRTIGQRHYDVQVMGATVLHQGKIAEMKTG
                     EGKTLVSTMPIYLNALAGDGVHVVTVNDYLAKRDSEWMGPIYRALGISVGLIQSQMRP
                     EERRPNYAADVTYGTNNEFGFDYLRDNMAMRREDMVQRGHHFAIIDEVDSILIDEART
                     PLIISGPVQDSAKWYQTFARIAPRLKRDQDYEIDESKKTVAVSEDGVARVEDYLGIEN
                     LYEHVNTPLVHHLQNAIRAKELYKRDVDYIVQDGEVLIVDEFTGRVLHGRRYSEGLHQ
                     AIEAKEGVRIKEENQTLATITIQNYFRMYEKLGGMTGTAKTQAAEFEQVYKLPVVTIP
                     TNRDMIRRDENDLIFKSEDAKWTALIDDIVERNEKGQPILVGTVSIEKSEHVSKLLQR
                     RGVTHNVLNAKQHEKEATIIAQAGQKGAVTVATNMAGRGVDIMLGGNPEYLARQDMAA
                     KGWDNDRYLLFEMNPEERKGYEEEYGPLYERHKQTTDAEHEVVVDLGGLYVLGTERHE
                     SRRIDNQLRGRSGRQGDPGESRFYLSLGDDLMRLFASDRIGSIMNRLNWPEDEPITAK
                     MVTRAIESAQRQVEGVNFERRKNVLKYDEVMNTQRKIVYADRRRILEGESLRDRAADM
                     VGEVIQGMVAEYVSPEVFAEDWDLEGLLTQIHLTYPSDLTIADLKPIEDPLELSEKVL
                     QEALEAYEKREAELGEETMRELERMVFLNIIDNLWREHLQEMDYLQEGIGLRAYGQRD
                     PLVEYQNEAHAMFEAMKATMIEEFVRYIYRVEVVREDEPSRPRPQRMVSEHGDRQESA
                     SPGAGTSGKVPRNAPCPCGSGKKYKKCHGAA"
     gene            complement(3733..4986)
                     /locus_tag="VF058_00085"
     CDS             complement(3733..4986)
                     /locus_tag="VF058_00085"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6206735.1"
                     /translation="MAVVRDDRAGAVRRATLLLAGAQMALWAGNGAYAAFGPLSIEEL
                     TGHEGTAAQLFGVYALAAAASALVTGRAMDRLGRRPGLLGGYVLNAVAGLLAFAAVRA
                     GSAPLLYAAGGLLGAGTAAALLGRAAVADMYPQERRGRAVGLLLVAGTFGAIGGPLLA
                     GVIHTLASSAGVAEPLAFPWLLVPVVALVAAGLVAAIRPDPRELAAPGPAPTAAPRRP
                     REILRLRPAATAVVAIGIAHAVMVTFMAQLPSSIHRHGASEVTVSIVVSLHLAGMFAF
                     SPAFGGLIDRWGRRAGLLVGAALSASGVLLGVAFPRPIGAGTGLLLIGMGWSASYLGS
                     TAAVSDMATPWERGGALGVTDLVASLASGVGAIAGASLLRAAGLPALGAAGLALLGLV
                     VVLLVPLREPSPGRWEPQRTASPTV"
     gene            complement(5019..5711)
                     /locus_tag="VF058_00090"
     CDS             complement(5019..5711)
                     /locus_tag="VF058_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005316851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6206736.1"
                     /translation="MSKKAAEKAGGDALKVLIVDDHALFRRGIQMVLEQEEGIEVVGE
                     ATDGLEAVEKAEELMPDVVLMDVRMPKRTGIEATSAIKDAIPHAKILMLTVSDEEEDL
                     YEAIKAGASGYLLKEISTEDVADAIRSVWTGQSRISPAMASKLLNEFQAMSKRAEERE
                     SVHTPRLTDREMEVLRLVAKGMNNRDIAKELFISENTVKNHVRNILEKLQLHSRMEAV
                     VYAVREKLLEIK"
     gene            complement(5692..6072)
                     /locus_tag="VF058_00095"
     CDS             complement(5692..6072)
                     /locus_tag="VF058_00095"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEX6206737.1"
                     /translation="MDVVVKATKGRVPARARQVVERKAARLARHDWRIRRVEVNVIQE
                     ANPRVSAGHRVEGSAWTDRRSFHASGAGETLEVAVEQVVSRLDRQLTGHAGKRRRKLI
                     EGANRVKSRQVPEPPEVMDVEEGR"
     gene            complement(6362..7066)
                     /locus_tag="VF058_00100"
     CDS             complement(6362..7066)
                     /locus_tag="VF058_00100"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HEX6206738.1"
                     /translation="MLDAIFPPRCLGCSRRGVLLCERCVENVGVIVPPLCERCGRPWA
                     APVEACADCPPGGIDLARSPFLYEGPIAAAIRGMKFGGWRALAPRLAAAIGAVLPEPG
                     VDAVCWVPLSARRRRRRGFDQAEVLGRRVARLLGLPAAGLLRRRRDTAAQARLGGAER
                     RAALVGAFRARRRVPRSVLLVDDVLTTGATAAACAAALREGGADRVVVATAARSVRGP
                     FPAGTFGGSPGPARRL"
     gene            complement(7102..7809)
                     /locus_tag="VF058_00105"
     CDS             complement(7102..7809)
                     /locus_tag="VF058_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206739.1"
                     /translation="MGDGDLSGVSVDAVFERFPASVRGAVVVRGVDPDPHQVRLTQAV
                     VVEVGPSRAVRNLELGQVTVDVAPRDRVMIPFEVPFADLEPGWYTVQAEVDVDGQESE
                     RGPREAERRFCVAWPSEAVRRGSVEAGLRIKVPGSRGAAIDRLVLRADSAAVHWRHAA
                     AQEPGFREFGDLKVTAGRRRLHVLDDEYEWSTGSRTTTIYPVLRGDEELTFELDRRHR
                     QGKPIQRGPWSATLSLP"
     gene            complement(7791..9038)
                     /gene="ahcY"
                     /locus_tag="VF058_00110"
     CDS             complement(7791..9038)
                     /gene="ahcY"
                     /locus_tag="VF058_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX6206740.1"
                     /translation="MCDVKDPSLAETGRERIEWAGREMRVLGLIRERFEKERPLDGLR
                     VAACLHITTETANLLRTLRAGGAEVAACASNPLSTQDDVAAALCDEGIATFAIRAEAN
                     DAYYAHIRSVLDTRPHVTMDDGADLVNVLHEERAEQAGEVRAGTEETTTGVIRLRAMA
                     KAGALRYPIVAVNDAMTKHLFDNRFGTGQSTIDAIMRATNFLLAGRTVVVCGYGMCGR
                     GVASRARGMGSQVVVTEVSPLAALEAAMEGYRVMPMREAARVGDIFVTVTGDTSVIRE
                     EHLQAMKDGAVLANAGHFDVEIDKEALSRLGGEPRRLRGSVDEYTMPDGRRIALLAEG
                     RLVNLGAAEGHPAAVMDMSFANQALVVEWLAGREGLEPEVLTVPDEIDAEVAALKLRA
                     MGIEIDELTPEQERYLGSWEMGT"
     gene            complement(9042..>9525)
                     /locus_tag="VF058_00115"
     CDS             complement(9042..>9525)
                     /locus_tag="VF058_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX6206741.1"
                     /translation="ASTGGLDPDLEPGSLVIASDHLNLLGENPLRGWRDEEGRPVFVD
                     LTRVYPPEPAEAAERAAREVGLPFARGVYAAMPGPTYETPSEAEMLRRLGGDVVGMSV
                     VPEACAAAALGMRCLGLFCVTNRVGVHVSHEEVTRVAAAFADGLRELLRRVLPALGEE
                     "
BASE COUNT         1292 a         3560 c         3234 g         1439 t
ORIGIN      
        1 gacgatggac cccgcgctcc gccgagagga tgccgtaggc gttcttcccc ttcacggtga
       61 cggtcgccga cttgatgccc gcctcctcgc cggggctcac ctcgtcgagc tccacctcga
      121 acccctcacg ctccgcccac cggaggtaca tccgaaggag catctccgcc cagtcctgcg
      181 actcggtccc gccggccccc gcgtggagcg aggcgatcgc gtcggcgtcg tcgaactccc
      241 ccgagaggag ggcggccagc tcgaggcggt ccacgtcggc cgccagcgac tcgaggtgct
      301 ccttcagctc ccgctcggtg tcggggtccg cctgctcgtc gaggagctgg tccagcacct
      361 cggcctcctc gagccgctcg gcgaggccct cgtagcgctc gacgtcgtcg cggaggcggg
      421 cgagcctggt ggtcagctgc tgggcgcgag catggtcgtc ccagagggac gggtcggaga
      481 gccgctcctc cagctcggcc atctccgctc gcttggcgtc gaggtcaaag gtactccttc
      541 gcgctcgaga ggcgctggga gaggccccgg atgcgctccg cgtgctcgct catggcgctc
      601 cgaatggtag cggatgccgg gggtcgcgaa atggtcccga atgactatag agacctggtc
      661 agcggcgccc gatactggcc ttatgaggcg ctcctctcgg tccgcggccc gcgatcgcct
      721 gcacgacgca ctggaggctc tgcagcgcga cggcgagccg gtgatcctcc gcgacggccg
      781 gcgcccggtc gccgtcctca tcaccgtgga cgactaccgg aagcgcttct cgcggatcca
      841 tgacgagagc accccgaacg gcgacgggcc cacctggccc caggtggagc ggcgggaggg
      901 cgaccgtcgg cgcggcgagc ggaggggccg ccggagggac cccgcccccc gcgaggggga
      961 ccccgggggc accctcgacg tgctccgcga gctccgctcc ctcggcctca cggccgagcg
     1021 ctagccactc gccgcgctca ggcggcgccg tggcacttct tgtacttctt gccgctgccg
     1081 caggggcacg gggcgttccg ggggaccttg cccgaggtcc cggcgcccgg cgaggccgac
     1141 tcctgccggt cgccgtgctc ggacaccatc cgctgagggc gcgggcgcga tggctcgtcc
     1201 tcccgcacca cctcgacgcg gtagatgtac cggacgaact cctcgatcat cgtggccttc
     1261 atcgcctcga acatcgcgtg ggcctcgttc tggtactcga cgagggggtc gcgctggccg
     1321 taggcccgca gcccgatccc ttcctggagg tagtccatct cctggaggtg ctcccgccag
     1381 aggttgtcga tgatgttgag gaacaccatc cgctccagct cccgcatcgt ctcctcgccc
     1441 agctcggcct cgcgcttctc gtaggcctcg agggcctcct ggagcacctt ctcggacagc
     1501 tcgaggggat cctcgatcgg cttgaggtcg gcgatcgtca ggtcgctggg gtaggtgagg
     1561 tggatctggg tcagcagccc ctcgaggtcc cagtcctcgg cgaagacctc ggggctcacg
     1621 tactcggcga ccatcccttg gatcacctcg cccaccatgt ccgccgcccg gtcccggagg
     1681 ctctcgccct ccaggatccg gcgccggtcc gcgtagacga tcttccgctg ggtgttcatc
     1741 acctcgtcgt acttgaggac gttcttccgc cgctcgaagt tgacgccctc gacctggcgc
     1801 tgggcgctct cgatggcgcg cgtcaccatc ttggccgtga tgggctcgtc ctcgggccag
     1861 ttcaggcggt tcatgatcga gccgatgcgg tccgaggcga acagccgcat cagatcgtcc
     1921 ccgagcgaga ggtagaagcg ggactccccc ggatcgccct ggcgccccga ccgaccccgc
     1981 agctggttgt cgatccggcg cgactcgtgg cgctcggtgc cgaggacgta gaggccgccg
     2041 aggtccacca ccacctcgtg ctcggcatcg gtggtctgct tgtggcgctc gtacagcggc
     2101 ccgtactcct cctcgtatcc cttccgctcc tccgggttca tctcgaagag gaggtaccgg
     2161 tcgttgtccc agcccttggc cgccatgtcc tggcgggcga ggtactcggg gttgcccccc
     2221 agcatgatgt ccacgccccg gccggccatg ttggtggcga ccgtgacggc gcccttctgg
     2281 cccgcctggg cgatgatcgt cgcctccttc tcgtgctgct tggcgttgag gacgttgtgg
     2341 gtcacgcccc gccgctggag cagcttcgag acgtgctccg acttctcgat cgagaccgtg
     2401 cccacgagga tcggctggcc cttctcgttc cgctcgacga tgtcgtcgat cagcgcggtc
     2461 cacttcgcgt cctcggactt gaagatgagg tcgttctcgt cgcgccggat catgtcgcgg
     2521 ttcgtaggga tcgtgacgac gggaagcttg tagacctgct cgaactcggc ggcctgggtc
     2581 ttggccgtcc cggtcatccc cccgagcttc tcgtacatac ggaagtagtt ctggatcgtg
     2641 atcgtggcca gggtctgatt ctcctccttg atccggacgc cttccttggc ctcgatcgcc
     2701 tggtggaggc cctcggagta gcggcgaccg tggaggaccc ggccggtgaa ctcgtccacg
     2761 atcagcacct cgccgtcctg gacgatgtag tccacgtccc gcttgtagag ctccttggcc
     2821 cggatcgcgt tctgcaggtg gtggacgagc ggggtgttga cgtgctcgta gaggttctcg
     2881 atcccgaggt agtcctcgac gcgggccacg ccgtcctccg acaccgccac cgtcttcttc
     2941 gactcgtcga tctcgtagtc ctggtcgcgc ttgagcctcg gggcgatgcg ggcgaaggtc
     3001 tggtaccact tcgctgagtc ctggaccggg ccgctgatga tcaggggggt gcgggcctcg
     3061 tcgatcagga tcgagtccac ctcgtcgatg atcgcgaagt ggtggccgcg ctggaccatg
     3121 tcctcgcgcc gcatggccat gttgtcccgg aggtagtcga agccgaactc gttgttggtg
     3181 ccgtaggtga cgtcggccgc gtagttgggg cgccgctcct cgggccgcat ctgcgactgg
     3241 atcagcccca ccgagatccc cagcgcgcgg tagatcggcc ccatccactc ggagtcgcgc
     3301 ttggccaggt agtcgttgac ggtgacgacg tggacgccgt cgccggccag cgcgttgagg
     3361 tagatcggca tcgtggagac gagggtcttc ccctcgccgg tcttcatctc cgcgatcttc
     3421 ccctggtgga ggacggtggc ccccatgacc tggacgtcgt agtggcgctg gccgatcgtg
     3481 cggcgggcgg cctcccggac ggcggcgaag gcctcgggga ggaggtcgtc gagcgtctcg
     3541 ccctccttca ggcgcgcccg gagctcgtcg gtcttgcccc ggagctggtc gtcggtgagg
     3601 tcctccgtct cgggctcgta cgaggagacg agctcgaccc aatgggagag ccgcttctgc
     3661 tcgcgcttct caccgatctg ctggaccttg ctgaagacca cctgaggacc ctccctgcgg
     3721 actggcttcc gattatacgg tcgggctcgc cgtgcgctgc ggctcccagc gtcccgggct
     3781 cggctcgcgg agcggcacga gcaggaccac gacgagcccg aggagggcca ggccggcggc
     3841 cccgagggcc gggaggcccg cggcccggag caggctcgcg cccgcgatcg cgcccacgcc
     3901 cgacgcgagc gaggcgacca ggtcggtcac gccgagcgct cctccacgct cccagggcgt
     3961 ggccatgtcg ctcacggccg cggtcgaccc gaggtaggag gccgaccacc ccatgccgat
     4021 caggaggagc ccggtccccg cgccgatcgg ccgggggaac gccacgccca gcagcacgcc
     4081 cgaggcactg agcgcggccc ccaccaggag gcccgccctc cgaccccacc ggtcgatcag
     4141 ccccccgaac gcgggcgaga acgcgaacat gccggcgaga tggaggctca ccacgatcga
     4201 gacggtcacc tcgctcgcac cgtgacggtg gatcgaggac gggagctggg ccatgaacgt
     4261 caccatgacg gcgtgggcga tcccaatcgc caccacggcc gtggccgccg ggcggaggcg
     4321 gaggatctcg cggggcctgc gcggagcggc ggtcggcgcg ggccccggag cggcgagctc
     4381 ccgggggtcc gggcggatcg ccgcgacgag ccccgcggcc accagcgcga cgacggggac
     4441 gagcagccac gggaaggcca gcggttccgc cacccccgcc gaggaggcga gcgtgtggat
     4501 cactccggcg agcagcgggc ctccgatggc gccgaaggtg ccggcgacga ggaggagccc
     4561 cacggcacgg ccgcggcgct cctgcgggta catgtcggcc accgcggctc tccccaggag
     4621 cgccgcggcc gtgccggctc ccagcaggcc ccccgccgcg tagaggagcg gagccgagcc
     4681 ggcccggacc gccgcgaagg cgagcaggcc ggccaccgcg ttgagcacgt agccgccgag
     4741 gaggccgggc ctccggccca ggcggtccat ggccctcccc gtcacgagcg cgctcgccgc
     4801 cgccgcgagc gcgtagacgc cgaacagctg ggccgcggtg ccctcgtggc cggtgagctc
     4861 ctcgatcgac agcggcccga acgccgcgta cgcgccgttc ccggcccaca gcgccatctg
     4921 ggcgccggcc aggagcagcg tggcgcgccg aaccgcgccc gcgcgatcat ctcgaacgac
     4981 ggccaacccg aatcctcccc ctcgaacgcc ggccgagcct acttgatctc gagcagcttc
     5041 tcgcgcaccg cgtataccac ggcctccatc ctggagtgga gctggagctt ctccaggatg
     5101 ttgcggacgt ggttcttcac ggtgttctcg gagatgaaca gctccttggc gatgtcgcgg
     5161 ttgttcatcc ccttggccac gaggcgaagg acctccatct cgcgatcggt gagccggggc
     5221 gtgtggacgc tctcccgctc ctcggcccgc ttcgacatcg cctggaactc gttgagcagc
     5281 ttcgacgcca tggctgggga gatgcgcgac tgcccggtcc agaccgagcg gatggcatcg
     5341 gccacatcct cggtggagat ctctttcagg aggtagccgc tggcgccggc cttgatggcc
     5401 tcgtagaggt cctcctcctc gtcgctcacc gtgagcatga ggatcttggc gtgcgggatc
     5461 gcgtccttga tcgcggaggt ggcctcgatc ccggtgcgct tcggcatgcg gacgtccatc
     5521 aggacgacgt ccggcatgag ctcttcggcc ttctcgaccg cctccaggcc gtcggtggcc
     5581 tcgcccacga cctcgatccc ctcctcctgc tcgaggacca tctggatgcc gcgacggaac
     5641 agcgcgtggt cgtcgacgat gaggaccttc agcgcgtcgc cgcccgcctt ctcagcggcc
     5701 ttcttcgaca tccatcacct cggggggctc cgggacctgg cgggatttta cccgattcgc
     5761 tccctcgatc agtttcctgc gccgcttccc ggcgtggccc gtcagctggc gatccagacg
     5821 cgagacgacc tgctccaccg ccacctcgag ggtctccccg gcgccgctgg cgtggaagga
     5881 tcgccggtcg gtccaggccg acccctccac ccggtgcccg gcgctcaccc tcgggttcgc
     5941 ctcctggatc acgttcacct cgacgcgccg gatcctccag tcgtggcgcg ccaggcgcgc
     6001 cgccttacgc tccacgacct gacgagcccg agcgggaacg cggcccttcg tcgccttcac
     6061 aacgacgtcc atggcacccc tcctccgcgg gcccccccac ctccggggag acggacgaga
     6121 tggaacccgc gcccggctga cctggtcttt gcccccacac tcgcctgctg aggggttcgc
     6181 cgcccggagg acggcttagc accttctccc ggcgtaccgg ggcaggactt acatctaagc
     6241 cgcaccatgc tcagcaacca agggttgcct tacgtgggtc gtttcgcctg cggctggcat
     6301 cgactaccgg ggaacatccc cgggcaacca cagacccgga cacgggttcc atctccggcc
     6361 atcatagccg acgtgccggt cccggagaac cgccgaaggt cccggcgggg aagggacccc
     6421 ggaccgacct cgccgcggtg gccaccacca cccggtcggc ccctccctcc cgcagggccg
     6481 cggcgcaggc cgcggcggtg gctccggtgg tgagcacgtc gtccaccagg aggaccgagc
     6541 gcggaacccg gcgtcgggcc cggaaggccc ccacgagggc ggcccgccgc tcggcgccgc
     6601 cgagccgggc ttgggccgcc gtgtcgcgcc gtctccggag gaggcccgcg gcggggaggc
     6661 cgaggaggcg cgcgacacgg cggcccagca cctcggcctg gtcgaacccg cggcggcggc
     6721 ggcggcgggc cgacagcggg acccagcaga cggcgtccac ccccggctcg ggcagcacgg
     6781 cgccgatcgc ggcggcgagc cgcggcgcca gcgcccgcca ccccccgaac ttcatgccgc
     6841 ggatcgccgc cgcgatcggc ccctcgtaca ggaacgggct gcgggccagg tcgatccccc
     6901 cgggagggca gtcggcgcac gcctcgaccg gggccgccca gggccgcccg caccgctcgc
     6961 acagcggagg cacgatcacc ccgacgttct ccacgcatcg ctcgcagagc aggacgcccc
     7021 gccgcgagca gcccaggcac ctcggaggga agatcgcgtc gagcaccggg ccagcgtagc
     7081 gagggggtcc gacgcggacg ctcaggggag cgagagggtg gcggaccagg ggcctcgctg
     7141 gatcggcttc ccctgacggt gacgccgatc cagctcgaag gtcagctcct cgtccccccg
     7201 cagcaccggg tagatcgtcg tggtccgcga ccccgtgctc cactcgtact cgtcgtccag
     7261 gacgtggagc cggcgacgcc ccgccgtcac cttcaggtcg ccgaactccc ggaaccccgg
     7321 ctcctgggcc gccgcgtgcc gccagtggac ggccgccgag tccgcccgga ggacgagccg
     7381 atcgatcgca gcgccgcgac tccccggaac cttgatccgg agcccggcct cgacgctccc
     7441 ccgtcgcacg gcctcgctgg gccaggccac gcagaaccgg cgctcggcct ctcgcgggcc
     7501 tcgttcgctc tcctgtccgt ccacgtcgac ctcggcctgg accgtgtacc agccgggctc
     7561 caggtcggcg aacgggacct cgaacgggat catcacgcgg tcgcgcggag ccacgtcgac
     7621 ggtgacctgg cccagctcga ggttccggac ggcgcgcgag gggccgacct ccaccaccac
     7681 cgcctgggtg aggcgaacct gatgcgggtc ggggtcgacc ccgcgcacga ccaccgcgcc
     7741 ccgcacggag gcggggaacc gctcgaacac cgcgtcgacg ctgaccccgc tcaggtcccc
     7801 atctcccacg agccgaggta gcgctcctgc tcgggggtga gctcgtcgat ctcgatcccc
     7861 atcgcccgga gcttcagggc cgcgacctcc gcgtcgatct cgtccggcac cgtcagaacc
     7921 tcgggctcga ggccctcgcg accagccagc cactccacga ccagggcctg gttggcgaac
     7981 gacatgtcca tcacggccgc cgggtggccc tccgcggcgc ccaggttcac cagccgcccc
     8041 tcggccagga gggcgatgcg ccgcccgtcg ggcatggtgt actcgtcgac cgacccccgg
     8101 aggcgcctgg gctcgccgcc gagcctcgag agcgcctcct tgtcgatctc gacgtcgaag
     8161 tgcccggcgt tggccaggac cgcaccgtcc ttcatcgcct ggaggtgctc ctcgcggatc
     8221 acgctcgtgt cgccggtgac cgtgacgaag atgtctccca ctcgggctgc ctcgcgcatc
     8281 ggcatcacgc ggtagccctc catcgcggcc tccagcgcgg cgaggggaga gacctcggtc
     8341 accacgacct gcgatcccat gccccgggcc cgggaggcga ccccacgacc gcacatcccg
     8401 tagccgcaga caaccaccgt gcgccccgcc agcaggaagt tcgtggcccg catgatcgcg
     8461 tcgatcgtcg actgccccgt cccgaaccga ttgtcgaaga ggtgcttcgt catcgcgtcg
     8521 ttcacggcga cgatcgggta gcggagggcg cccgccttgg ccatggcccg gaggcggatc
     8581 acgccggtgg tggtctcctc ggtccccgcc cgcacctcgc cggcctgctc cgcccgctcc
     8641 tcgtgaagga cgttcacgag gtccgcgccg tcgtccatcg tgacgtgggg cctcgtgtcg
     8701 aggaccgacc tgatgtgcgc gtagtaggcg tcgttggcct cggcccggat ggcgaacgtg
     8761 gcgatgccct cgtcgcagag ggccgcggcc acgtcgtcct gggtcgagag cgggttcgag
     8821 gcgcaggccg cgacctcggc cccgcccgcg cgcagcgtcc gaaggaggtt cgccgtctcc
     8881 gtcgtgatgt gaaggcaggc ggcgacgcgg agcccgtcca ggggacgctc cttctcgaag
     8941 cgctccctga tgaggccgag gacgcgcatc tcgcgcccgg cccattcgat gcgctcccgg
     9001 cccgtctcgg cgagactcgg atccttgacg tcacacacgg ctcactcctc tcccagggcg
     9061 ggcaggaccc gccgcagcag ctcgcgcagg ccgtcggcga acgccgccgc cacccgcgtc
     9121 acctcctcgt ggctcacgtg gacccccacg cggttcgtga cgcagaagag gcccagacac
     9181 cgcatcccca gcgccgccgc ggcgcatgcc tcgggaacca ccgacatccc gaccacgtcg
     9241 cctcccagcc gccgcagcat ctcggcctcg gacggcgtct cgtaggtggg acccggcatc
     9301 gcagcgtaca cgcccctcgc gaacgggagt cccacctcgc gggcggcccg ttctgcggcc
     9361 tccgcaggct ccggagggta cacgcgcgtc aggtccacga acaccggccg accctcctcg
     9421 tcgcgccagc cccggagggg gttctcgccc aggaggttca ggtggtccga ggcgatgacg
     9481 agcgaccccg gctccaggtc ggggtcgagc ccgccggtgg aggcg
//
LOCUS       DASUTW010000005         3639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773 k141_1682,
            whole genome shotgun sequence.
ACCESSION   DASUTW010000005 DASUTW010000000
VERSION     DASUTW010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /gene="rpoD"
                     /locus_tag="VF058_00120"
     CDS             <1..1067
                     /gene="rpoD"
                     /locus_tag="VF058_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX6206742.1"
                     /translation="VSAEQMDNILQHLQDENIEVVEMVDELDAEEFAREARSARDEEL
                     ALKAPTNDPVRMYLKEIGKVPLLTAEQEVILAKAIDEGEAATAEIDKATDNGRKLTPT
                     RLRELQRTERHGQLAKKKLIEANLRLVVSIAKRYVGRGMLFLDLIQEGNLGLIRAVEK
                     FDYNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRIQRQLLQDLGR
                     EPTPEEIGREMEFSPEKVREILKVSQEPVSLETPIGEEEDSHLGDFIEDAEAIVPVDA
                     ASFILLQEQLESVLHTLSEREKKVIELRFGLMDGHPRTLEEVGREFGVTRERIRQIES
                     KTLSKLRHPSRSQKLRDYLE"
     gene            1121..1193
                     /locus_tag="VF058_00125"
     tRNA            1121..1193
                     /locus_tag="VF058_00125"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1154..1156,aa:Asn,seq:gtt)
     gene            complement(1203..2360)
                     /locus_tag="VF058_00130"
     CDS             complement(1203..2360)
                     /locus_tag="VF058_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019551191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX6206743.1"
                     /translation="MEEDAQQVEGQGPEAAAPESIVDEFNTWLGHLVNSDGSDLHVKV
                     GSPPMIRLRGILRRVEREPLTAGDTQALVAAIVPADRRRRLDEEGEVDFAHSLPGVGR
                     FRVNAFRQRGSISIVLRKLRFGGPEFAEIGLPEVCRRLSDEHRGLILVTGPTGSGKTT
                     TLSAMIEHINKTRPVHIVTIEDPIEVVFEDKVASINQREVGLDTQSFLGALRVALRQD
                     PDVILIGEMRDPETVRTALQAAETGHLVLSTLHTLDATETVNRVVDFFPPHQQQQIRF
                     TLAGSLRGILSQRLLPAIDGDRVPAVEVLVNTGRIQERIIDASMTAEIREVIQEGEYY
                     GMQTFEQSLLQLVQDGQVAVEEAMVATTEPHDFRLMLEQAGITEVGALKTV"
     gene            2510..2962
                     /locus_tag="VF058_00135"
     CDS             2510..2962
                     /locus_tag="VF058_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206744.1"
                     /translation="MDDLRAARDRVDRRDSPSPRSMVVPWALIVIWGVVASVLATWLY
                     LEREAATEELASARVEVAELRRMLVEAEGRIDELREGLQDQQDEVGRRGESIEACREA
                     LERISAAWSDVARAVQAAGELDGEEARSFAAEAERQRRAAQRAARRCR"
     gene            3133..3633
                     /locus_tag="VF058_00140"
     CDS             3133..3633
                     /locus_tag="VF058_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206745.1"
                     /translation="MGMRRILFPASVLAVVALLVVPQALIAHRPTGGSALDEQTFKFR
                     GRDVATSSRAWRRVPGFRFRVCATREVSANVNLVLRGAPALIRFRIDGSDELIAHPGP
                     VRFRPGSGRVFSFTFAEEVFPFEAEDLHFFDLEWRSPTGKRVILKKGMANVLFERAFG
                     GPPCEP"
BASE COUNT          573 a         1242 c         1249 g          575 t
ORIGIN      
        1 acgtctcggc cgagcagatg gacaacatcc tgcagcacct gcaggacgag aacatcgagg
       61 tcgtcgagat ggtcgacgag ctcgacgccg aggagttcgc ccgcgaggcc cggtcggccc
      121 gcgacgagga gctggccctc aaggccccca ccaacgaccc cgtccggatg tacctcaagg
      181 agatcggcaa ggtcccgctc ctgaccgcgg agcaggaggt catcctcgcc aaggcgatcg
      241 acgagggcga ggccgccacc gccgagatcg acaaggccac cgacaacggc cgcaagctca
      301 ccccgacccg cctgcgcgag ctccagcgca ccgagcgcca cggccagctg gccaagaaga
      361 agctcatcga ggccaacctg cgcctggtcg tgtcgatcgc caagcggtac gtgggccggg
      421 gcatgctgtt cctcgacctc atccaggagg gcaacctcgg cctcatccgg gccgtggaga
      481 agttcgacta caacaagggc ttcaagttct ccacctacgc cacctggtgg atccgccagg
      541 ccatcacccg ggccatcgcc gaccaggccc gcaccatccg catcccggtg cacatggtcg
      601 agacgatcaa caagctgatc cgcatccagc gccagctcct ccaggacctg ggccgcgagc
      661 ccacccccga ggagatcggc cgggagatgg agttctcgcc cgagaaggtc cgcgagatcc
      721 tcaaggtgtc ccaggagccg gtgtcgctgg agaccccgat cggcgaggag gaggactctc
      781 acctggggga cttcatcgag gacgccgagg cgatcgtccc tgtggacgcc gccagcttca
      841 tcctcctcca ggaacagctc gagtccgtcc tccacacgct ctccgagcgc gagaagaagg
      901 tgatcgagct gcgcttcggg ctgatggacg gccatccccg cacgctcgag gaggtgggcc
      961 gggagttcgg cgtcacccgg gagcggatcc gtcagatcga gtcgaagacc ctctccaagc
     1021 tccgccaccc cagccgctcg cagaaactcc gcgactacct ggagtagggt gccggcggcc
     1081 gctggtacac tttgctttcg gacgctccac gcgcccgtct tccggggtag ctcaatcggt
     1141 agagcggccg gctgttaacc ggtaggttgc gagttcgagt ctcgcccccg gagctcccag
     1201 gctcagaccg tcttcagcgc gcccacctcg gtgatgccgg cctgctccag catcagcctg
     1261 aagtcgtgcg gctcggtcgt cgcgaccatg gcctcctcga ccgcgacctg cccgtcctga
     1321 acgagctgga ggagcgactg ctcgaaggtc tgcatcccgt agtactcgcc ctcctggatc
     1381 acttcgcgaa tctcggccgt catgctggcg tcgatgatcc gctcctggat ccgcccggtg
     1441 ttgacgagca cttcgaccgc ggggacccgg tcgccgtcga tcgcggggag gagtcgctgg
     1501 gagaggatcc ctcggagcga gcccgcgagc gtgaaccgga tctgctgctg ctggtgcgga
     1561 gggaagaagt ccacgacccg gttcaccgtc tcggtggcgt cgagggtgtg cagggtcgag
     1621 agcacgaggt gtccggtctc ggcggcctgc agcgccgtcc gcaccgtctc cgggtcccgc
     1681 atctccccga tcaggatcac gtcgggatcc tggcgcaggg ccacgcggag ggcgccgagg
     1741 aacgactggg tgtccagccc gacctcgcgc tggttgatcg atgccacctt gtcctcgaac
     1801 acgacctcga tcgggtcctc gatcgtgacg atgtgcacag gccgagtctt gttgatgtgc
     1861 tcgatcatcg ccgagagcgt ggtggtcttc cccgacccgg tcgggccggt gacgaggatc
     1921 aggccgcggt gctcgtcgga cagcctccgg cacacctcgg gcaagccgat ctccgcgaac
     1981 tcgggtcccc cgaaccgcag cttcctgagc acgatgctga tcgagcctcg ctggcggaag
     2041 gcgttgacgc ggaaccgccc cacgccgggg agggagtgcg cgaagtcgac ctccccctcc
     2101 tcgtcgagcc ggcggcggcg gtcggccggg acgatcgcgg ccacgagcgc ctgcgtgtcg
     2161 cccgccgtga gcggctcgcg ctcgacgcga cggaggatgc cccgcaagcg gatcatcgga
     2221 ggtgacccca ccttgacgtg gaggtcggac ccgtcgctgt tcacgaggtg gcccagccag
     2281 gtgttgaact cgtcgacgat gctctcgggc gcggcggcct cgggtccctg cccctcgacc
     2341 tgctgcgcgt cctcttccat tcccatatcg gctgtcatga tggatcgagc cccctgctct
     2401 cgcgagcctc ggacggcggg acgtcgtgca cccgcctcac ccccatccca tcggaccgtg
     2461 ggggggcctt cttgagcctg tcgacccccg cccctatgct tcggctgcca tggacgatct
     2521 ccgcgccgcc cgagaccgtg tcgatcgccg tgactcgccg agcccgcgat ccatggtcgt
     2581 tccgtgggcg ctgatcgtga tctggggggt ggtggcctcg gtcctggcca cctggctcta
     2641 cctcgagcgg gaagccgcga ccgaggagct cgcgtcggcg agggtcgaag tggcggagct
     2701 ccgccggatg ctcgtcgagg ctgagggaag gatcgacgag ctgcgggaag gcctccagga
     2761 ccagcaggac gaggtgggca ggagagggga gagcatcgag gcgtgccggg aggccctgga
     2821 gcggatctcg gccgcgtgga gcgacgtcgc gcgggccgtc caggcggccg gagagctcga
     2881 cggggaggag gcgcgctcgt ttgcggcgga ggcagagcgg caacggcgcg cggcccagcg
     2941 ggccgcgcgc cgctgccgct gaccgccctc cggaacagca cctgcggggg ccgtagctcg
     3001 ttcggtccgg cagggaagtc cgatcccacc taccggctcc acggacgtgc cgccggctcc
     3061 acaggagcag aggcttggaa ggcctccttc gcaggcgccg cattagaatc gaggaactca
     3121 taggaggagg tgatgggaat gcgcaggatc ctcttcccgg cctcggtcct ggcggtggtg
     3181 gccctgctcg tcgttcctca ggcgctcatc gcccaccggc caaccggtgg ttcggcgctc
     3241 gacgagcaga ccttcaagtt ccgcggacgc gacgtcgcca cgtcaagcag agcttggcga
     3301 agggtgccgg ggtttcggtt ccgggtttgt gccacgaggg aggtgagcgc gaacgtcaac
     3361 ttggtccttc gaggcgctcc ggctctgatc cgcttccgga tcgacggcag cgacgagctg
     3421 atagcccacc ctgggccggt gaggttccgt ccgggctccg ggcgggtgtt ctcgttcacc
     3481 ttcgcggagg aggtcttccc gttcgaggct gaggatctcc acttcttcga tctcgagtgg
     3541 cgatcgccga ccgggaagcg cgtcatcctg aagaagggga tggcgaacgt gctgttcgag
     3601 cgagcgttcg gggggcctcc ctgcgagccg tgagctcgg
//
LOCUS       DASUTW010000006         8462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1815982, whole genome shotgun sequence.
ACCESSION   DASUTW010000006 DASUTW010000000
VERSION     DASUTW010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8462
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815982"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..768
                     /locus_tag="VF058_00145"
     CDS             82..768
                     /locus_tag="VF058_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018500389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6206746.1"
                     /translation="MALRIVFAEDDYLVREGTAALLAQTEEVEVVATVPDRDALLTAV
                     KEHRPDAVLTDIRMPPSRTTEGIEAARTIRAEHPSTGVVVLSQYAEEEYAYELLKDGA
                     EGLGYLLKERVGDIEEVVRALHDVAQGGSVLDPKIVEGLVARRSREERSPLAHLTDRE
                     REVLALMAEGRNNAAIAASLFVTERAIEKHINSLFHKLGLSEEPNLHRRVMAVLTFLR
                     ENGPRPDAGD"
     gene            887..2956
                     /locus_tag="VF058_00150"
     CDS             887..2956
                     /locus_tag="VF058_00150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX6206747.1"
                     /translation="MTSRISARVAWSAVVLGTWLVGMAIALQILREGAGLGLVENLLF
                     AAIFISMGSVGALVVGREPRNAVGWILLGVAVLPALAFFSEMYARYGQTVAAGSLPAL
                     AWGWWLGDWTWMGLLAMIATLPLVFPDGRVPSARWKPYLWLVLSMALITAVLFALDPA
                     SYPGGRHTNPIGVEALRPVADFIEGPGYLLFVGLFLGSVASLVFRFRRAGREQRQQIK
                     WFLFAVAVLVTLFVLDGVFQALGLSDLEAVNLWVNTVLSAAAFASLPVGLGIAILRHR
                     LYDIDVVISKTVVYGALAAFVTLVYVGIVVGIGALVGTRGNLFLSIVATAVIALAFQP
                     VRRWAGHLANRLVYGKRATPYEVLSRLAQGMGSQHTADDALPSLARVLAEGTGAARAE
                     VWLRVSGSLRLGAAWPDVGSQVEDIAIVDESLPEIRTVEAAVPVAHDGDLLGALGVSP
                     ARGEALTPTTEKLLADVGAQAGLLLRNVRLFEELRASRQRLVAAQDEERRRLERNIHD
                     GAQQQLVALAVKLRLIEAMIEKDPVKARELAAETKAESQEALENLRDLARGIYPPLLA
                     DKGLAEAIEAQARKAPFPVTVELNGVGRYPPEAEATAYFCVLEALQNTAKYAEASRAV
                     VSLGHDDGDLVFIVADDGKGFDPAATPRGAGLQNMADRLDALGGTVEISSEPGRGTTV
                     TGRIPVR"
     gene            2953..5061
                     /locus_tag="VF058_00155"
     CDS             2953..5061
                     /locus_tag="VF058_00155"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX6206748.1"
                     /translation="MSARVARWLAWVTFGLFVVIALSGSYLQELNVSAGEGREYYLLA
                     AFIVFAGFGAFIASRVPRNAIGWLYLAVALLVGLGFAGAEYAEYAVLTSPGSLPAPRL
                     AGWFGQWTWLPAVGLIPTFLFLLFPDGHLPSRRWLVVAWLSAAVIAAMFVLGAFTEGP
                     MGDIHPPLENPVGFLPNPSGSASPAEDAPLIPALLLLALASIASVFFRYRRASIEQRQ
                     QLKWFLYAGGLVTLSVAVGDVVPDPWSNVLFAVMLLSLPVATAVAILKYRLYDVDVVI
                     NKTVVYGMLAAFVTLVYVGIVVGIGSLVGSRGNVFLTILATAIIALAFQPARVRARRL
                     ANRLVYGKRATPYEVLSRLAHGMGSEYSADDALPSLARVLGEGTGAARAEVWLRVGSN
                     LRAAASWPDDVEPRQRSLALADGDLPPIVGVDLTLPVKHEGELLGALAISMPRGETVT
                     PDTERLLGDVASQAGLVLRNVRLIAELRASRQRLVAAQDEERRRLERNIHDGAQQQLV
                     ALSVKVRLAETLAGKEPEKAAELMAQVRAETQQALDELRDLARGIYPPLLADKGLAAA
                     LEAQARKVPFPVRVEPNGIGRYPPETEANAYFCVLEALQNAAKYAAASAAVVRLSEET
                     GHLLFVVSDDGVGFDPETTPRGSGLQNMADRLEALGGTVEVSSAPGEGTTVRGRIPVQ
                     AIAADQAFSSRSGSNSDLGM"
     gene            complement(4995..5366)
                     /locus_tag="VF058_00160"
     CDS             complement(4995..5366)
                     /locus_tag="VF058_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6206749.1"
                     /translation="MSVRVLIVDDQEPFRMAARAVVELTEGFEVVGESETGEDAVEAA
                     RGLQPDLVLMDVNLPGIDGLEATRRILAQAAGRVVVVMVSTYEAEEYAPRAAEVGAAA
                     YIPKSEFEPERLENAWSAAIA"
     gene            5531..6403
                     /locus_tag="VF058_00165"
     CDS             5531..6403
                     /locus_tag="VF058_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6206750.1"
                     /translation="MATLQHLDDVDLAYEVEGAGTPLVLLHGSWTDRRSWDLVTDRLA
                     RDFTVVRYDRRGHSESPPATGSFDDARDLSALIDDLDLGPVHVAASSSGGVIALRLAA
                     HRPELLRSIVSHEPPLTSLIPEGDGARPIVDDAVAQEQTVGAQIEAGDHAGAARRFVE
                     ELALGPGAWDMLPEALRSTMVRNAATFAEEVRDPSSRWVDLDSLRGFQRPVLLTEGGV
                     GPGWYGAAIDRLAEALPAVSRHCYSEAGHMPHASHPEEYVEVVGGFLRMVDASAAALS
                     RAAPRRPSLGRRSP"
     gene            complement(6354..7559)
                     /locus_tag="VF058_00170"
     CDS             complement(6354..7559)
                     /locus_tag="VF058_00170"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6206751.1"
                     /translation="MTPDRISEAELAERAGISVEHLRELAELGIVEPEDGTVPRRDVM
                     RVRVVLELEAKGMDPASLGAAVAGGHLTLGYLESAGRRFPRSDRTLAELGEEIGIPLE
                     TLQAAYVAFGLSRPAADELVREEDLPVLRALPVLFGAGVGEGEVLRAVRVWGDSARRV
                     AQFQTHYFHHTIEEPFRARGLPDNQAFEAAIREVGLRIGHSGEEMLGWLFRRHSERFL
                     TEHQFEHVETALEEAGIRPRSPKGAEAVAFADLSGYTQLTEEAGDEVAARVSISLAQL
                     VNEIASRHDGEVVKMLGDGVLFHFADPGDSIRASLDLVDAVRPRGLPPAHVGINAGPM
                     IYDEGDYFGRTVNVAARIASVATADQVFVGEDVLREVTPEEFRLVEVGGFALKGIADP
                     VKVYEALPD"
     gene            complement(7563..7712)
                     /locus_tag="VF058_00175"
     CDS             complement(7563..7712)
                     /locus_tag="VF058_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011222411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEX6206752.1"
                     /translation="MAMVTDPVCGMRIDPEDAAATAEYEGTKYYFCSEACHEAFLEDP
                     ESYVS"
     gene            complement(7743..>8462)
                     /locus_tag="VF058_00180"
     CDS             complement(7743..>8462)
                     /locus_tag="VF058_00180"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX6206753.1"
                     /translation="EQVERGWEAAKGFFGRLPARNCEVRAVPPDLEEDALDYYQGPTD
                     DWSRPGIYWVNTAPRPLHSLAPTTYHEATPGHHFETALSVEAEGRHPIRRLGNELQGA
                     AFGEGWGLYSERLADEMGLYVDEYERLGMLEMQALRAARLVVDTGVHAFGWERERVVA
                     ELEGTGLARWMAEAEADRYIAMPGQALCYKVGQLEIERLREEASRRPGFRLADFHDRL
                     LEMGSVPLPTLRRELAAAGGA"
BASE COUNT         1185 a         3069 c         2906 g         1302 t
ORIGIN      
        1 cccggacgcc ctcgcggatc aagacgttgt cgtccccgag cacgacgcgg atcgtcatgg
       61 ctcccgtatt accatccgcg ggtggccctg cgcatcgtct tcgccgagga cgactacctc
      121 gtgcgcgagg gcacggccgc cctgctcgcg cagaccgagg aagtcgaggt ggtcgccacg
      181 gtccccgatc gggatgcgct gctgaccgcc gtcaaagagc accggcccga cgccgtcctc
      241 accgacatcc ggatgccgcc cagcaggacc accgagggga tcgaggccgc ccggacgatc
      301 cgcgctgagc acccgagcac gggcgtggtg gtcctctccc agtacgcgga ggaggagtac
      361 gcctacgagc tgctgaagga cggcgcggag ggcctcgggt acctgctgaa ggaacgggtc
      421 ggcgacatcg aggaggtggt ccgcgccctc cacgacgtgg cccagggcgg ctcggtcctc
      481 gaccccaaga tcgtggaggg gttggtggcc cgccgctccc gcgaggagcg gtcgccgctc
      541 gcgcacctga ccgaccgcga gcgggaggtg ctcgccctca tggccgaggg gaggaacaac
      601 gcggccatcg cagcgtccct cttcgtcacc gaacgcgcca tcgagaagca catcaactcc
      661 ctgttccaca agctcggtct ttccgaggag ccgaacctcc accggcgggt catggccgtc
      721 ctcaccttcc tcagggagaa cggtccacgt cccgacgcag gagactagcc agcccacagc
      781 acgggtccca cccggcctcc acgacgccag ggggtggtgg aggcaccttc tcgacgtggg
      841 gaggcccccc tttcccgcgt ctccctcgca gggcacaatc tcggacatga cgtcccgaat
      901 ctctgcgcgg gtcgcatggt cggccgtcgt gctgggcacc tggctggtcg gcatggccat
      961 cgccctgcag atcctccggg agggggcggg cctcgggctc gtcgagaatc tcctcttcgc
     1021 cgcgatcttc atctcgatgg gctccgtggg agctctcgtg gtggggaggg agccccggaa
     1081 tgccgtggga tggatcctcc taggggtggc agtcctgccc gccctggcct tcttctcgga
     1141 gatgtacgcg cgctacggac agacggtcgc cgccggctcc ctccccgccc tcgcctgggg
     1201 ctggtggctc ggggactgga cgtggatggg gctcctggcc atgatcgcca ccctgccgct
     1261 cgtgttcccc gacggaaggg tcccctcggc gcgatggaaa ccctacctgt ggctggtcct
     1321 gtcgatggcg ctcatcacgg ccgtgctgtt cgccctggat ccggcctcat atccgggcgg
     1381 tcgccacacg aatccgatcg gcgtggaggc gctccgacca gtggcggact tcatcgaagg
     1441 gcccggatac ctcctcttcg tcggcctctt cctgggctcc gtcgcgtcgc tcgtcttccg
     1501 gttccgccgc gccggtcgtg agcagcggca gcagatcaag tggttcctct tcgcggttgc
     1561 ggtcctcgtc acactcttcg tgctcgacgg cgtcttccag gctctcggac tgtccgacct
     1621 cgaggcggtt aacctctggg tcaacaccgt actgtccgcg gcggcgttcg ccagcctccc
     1681 ggtaggactc ggcatcgcca tcctgcggca ccgcctctac gacattgacg tcgtcatcag
     1741 caagacggtc gtctacggag ccctggccgc gttcgtgacg ctcgtctacg tcgggatcgt
     1801 cgtgggaatc ggggccctcg tcggcacccg aggcaacctg ttcctctcca tcgtggccac
     1861 ggccgtgatc gctctggctt tccagccggt gcggcggtgg gccgggcacc tcgcgaaccg
     1921 cctcgtctac gggaagcggg ccacgccgta cgaggtcctc tctcgcctgg cgcagggcat
     1981 gggatcgcag cacacggcgg acgacgccct ccccagcctg gcgcgggtcc tggccgaggg
     2041 gaccggcgcg gctcgtgccg aggtgtggct ccgggtgagc ggttcgctcc gcctcggcgc
     2101 cgcgtggccg gacgtcgggt cacaggtcga ggacatcgcc atcgtcgacg agagcctgcc
     2161 ggagatccga acggtcgagg cagccgtgcc ggtcgcgcac gacggcgacc tccttggcgc
     2221 gcttggggtc tcgcccgctc ggggggaggc cctgacgcct acgacggaga agctcctggc
     2281 tgacgtcggg gcccaggcgg ggctcctcct tcggaacgta cgactgttcg aggagctgcg
     2341 ggcatccagg cagcggctgg tggccgccca ggatgaggag cgccgccggt tggagcgcaa
     2401 catccacgac ggggcccagc agcagctcgt ggccctggcc gtgaagctca ggctgatcga
     2461 ggcgatgatc gagaaggacc cggtgaaggc cagggagctc gcggcagaga cgaaggccga
     2521 gtcgcaggag gctctggaga acctccggga cctggcccgc gggatctacc cacccctgct
     2581 ggctgacaag gggctggcgg aggccatcga agcccaggcc cgcaaagccc ccttcccggt
     2641 gaccgtggag ctgaacggcg tgggacgcta cccgcccgag gccgaggcca cggcctactt
     2701 ctgcgtgctg gaggccctcc agaacacggc caagtacgcc gaggcctcgc gagccgtcgt
     2761 gtcgctaggc cacgacgacg gcgacctcgt cttcatcgtg gccgacgacg ggaaggggtt
     2821 cgacccggcc gccaccccca gaggagccgg cctccagaac atggccgacc ggctggacgc
     2881 gctcggcggc accgtcgaga tctcctcgga gccgggacgg ggaacgacgg tcacgggacg
     2941 gatcccggtc cgatgagcgc tcgcgtcgct cgctggctgg cgtgggtcac gttcggcctc
     3001 ttcgtggtga tcgccctgag cggctcctat ctgcaggagc tgaacgtcag cgccggtgaa
     3061 ggccgcgagt actacctcct tgcggcgttc atcgtcttcg cgggcttcgg agccttcatc
     3121 gcctcgcggg ttccacggaa cgccatcgga tggctgtacc tcgcggttgc gttgctcgtg
     3181 ggcttggggt tcgccggcgc cgagtacgcc gagtacgcgg tcctgacgag ccctgggagc
     3241 ctgcccgctc cccggttggc gggctggttc ggccagtgga cctggctccc cgccgtgggc
     3301 ctgatcccga cgttcctctt cctgctcttc cccgacggtc acctgccgtc ccgccgatgg
     3361 ctggtcgttg cctggctctc cgcggcggtc atcgccgcca tgttcgtact gggtgccttc
     3421 accgagggac cgatgggcga catccaccca ccattggaga atcccgtcgg cttccttccc
     3481 aaccccagcg gatccgcctc gcccgccgag gacgctccgc tgatcccggc gctcctgctc
     3541 ctcgccttgg ccagcatcgc ttcggtgttc ttccgctacc ggcgggcatc gatcgaacag
     3601 cgtcagcagc tgaagtggtt cctctacgcg ggcggcctgg tcacgctgtc ggtcgccgtc
     3661 ggcgacgtcg tcccggaccc ctggtcgaat gtgctgttcg cggtgatgct cctgtcgctg
     3721 cccgtagcga cggccgtggc catcctcaag taccgcctgt acgacgtcga cgtcgtcatc
     3781 aacaagacgg tcgtctacgg gatgctggcc gcgttcgtga cgctcgtcta cgtgggcatc
     3841 gtggtgggga tcggctcgct ggtcgggagc cgtggcaacg tgttcctgac gatcctggcg
     3901 accgccatca tcgctctggc gttccagccc gcccgcgtgc gggccaggcg cctcgccaac
     3961 cggctggtct acgggaagcg tgccacgccc tacgaggtcc tgtcccgcct cgcccatggc
     4021 atggggtccg agtactcggc cgacgacgct ctcccgagcc tggccagggt cctcggcgaa
     4081 gggaccggag cggcccgtgc cgaggtctgg ctccgggtcg ggagcaacct gcgagccgcg
     4141 gcctcctggc cggacgatgt ggagccccgc caacgctccc tcgctctggc cgatggagac
     4201 ctacccccga tcgtcggggt ggatctgacc ctccccgtga agcacgaggg agagctcctc
     4261 ggcgcgctgg cgatctcgat gccgcgggga gagaccgtga ccccggacac cgagaggctc
     4321 ctgggcgacg tcgcgtcaca ggccgggttg gtgctccgga acgtccgcct gatcgcggag
     4381 ctccgggcgt cccgacagcg gctcgtggcc gcccaggacg aggaacgccg ccggctggag
     4441 cggaacatcc acgacggcgc tcagcagcag ctggtggcgc tctcggtgaa ggtgcgcctg
     4501 gcggagaccc tcgccgggaa ggagccggag aaggccgccg agctcatggc gcaggtccgc
     4561 gccgagaccc agcaggccct cgacgagctt cgcgacctgg ccagagggat ctatcccccg
     4621 ctgctggcgg ataaggggct ggccgcggcg ctggaggccc aggcccgcaa ggtcccgttc
     4681 cccgtgcggg tggagccgaa cgggatcggg cgataccccc cagagaccga ggccaacgcc
     4741 tacttctgcg tcctggaggc actccagaac gccgccaagt acgcggcggc ctccgcggcg
     4801 gtcgtccggc tctccgagga gaccgggcat ctcctgttcg tcgtgtccga cgacggagtc
     4861 gggttcgatc ccgagacgac gccgcgaggg tccgggcttc agaacatggc cgaccggctg
     4921 gaggcgctgg gcgggactgt ggaggtctct tccgcgcccg gggaggggac gacggtgagg
     4981 gggcggatcc cggttcaggc gatcgccgcg gaccaggcgt tctcgagccg ctcgggctcg
     5041 aactccgact tggggatgta ggccgcggcc cccacctccg cggcgcgagg agcgtactcc
     5101 tcagcctcgt aggtcgagac catgaccacc accacgcgcc ccgcggcctg ggcgaggatg
     5161 cggcgcgtgg cctccagccc gtcgatcccc gggaggttca cgtccatcag cacgagatcg
     5221 ggctggaggc cacgcgccgc ctcgacggca tcctcacccg tctcggactc gcccacgacc
     5281 tcgaagccct ccgtgagctc cacgaccgcc cgagccgcca tccggaacgg ctcctggtcg
     5341 tcgacgatca gcacgcgcac gctcatctcg gtctcagcgt ctcacagcga cctagcaccg
     5401 gaaaggatgc gggcaccacc ccggtgggag ggggcaaccc cacccgggat cgaggatggg
     5461 taggcctacc gatgcgctcg gatcccgtcg tctcgcaccc tgggaccacc cgatcgaagg
     5521 gaggatgctg atggcgacgc tgcagcacct ggacgacgtc gacctcgcct acgaggtcga
     5581 gggggccggg acgcccctgg tcctcctcca cggatcctgg accgaccgcc ggagctggga
     5641 tctggtcacg gaccggctcg cccgagactt caccgtcgtg cggtacgacc ggcgtggtca
     5701 cagcgagagc ccgcccgcga cggggtcgtt cgacgacgcc cgcgacctgt cggccctgat
     5761 cgatgatctc gacctcggcc cggttcacgt cgccgccagc tcgtcgggag gcgtgatcgc
     5821 cctccggctg gcggcccatc ggccggagct gctccgaagc atcgtctcgc acgaaccccc
     5881 gctcacctcg ctcatccccg aaggcgatgg ggcacgcccg atcgtcgacg acgccgtcgc
     5941 gcaggagcag acggtagggg cgcagatcga ggcgggggat cacgcgggcg cggcccgacg
     6001 attcgtcgag gagctcgccc tggggcccgg cgcctgggac atgcttcccg aggccctgcg
     6061 ctccaccatg gtccggaacg ccgcgacctt cgccgaagag gtgcgggacc caagctcccg
     6121 ttgggtggac ctcgactccc tcaggggctt ccagcgcccc gtactgctca ccgagggcgg
     6181 cgtcggtccg gggtggtacg gagcggcgat cgaccggctc gccgaagcgc tccccgcggt
     6241 ctcgcgccac tgctactcgg aggcggggca catgccgcac gcgagccacc ccgaggagta
     6301 cgtggaggtg gtgggaggct tcctccggat ggtcgatgcg tcggccgcgg cgctcagtcg
     6361 ggcagcgcct cgtagacctt cactgggtcg gcgatcccct tgagggcgaa gccgcccacc
     6421 tcgacgaggc ggaactcctc gggcgtcacc tcgcgcagca cgtcctcccc cacgaacacc
     6481 tgatccgccg tcgccacgga cgcgatgcgg gccgcgacgt tcaccgtacg tccgaagtaa
     6541 tcgccttcgt cgtagatcat cggccccgcg ttgatcccga cgtgggcggg cggcagccct
     6601 cgcggccgaa cggcgtcgac gaggtccagt gaggctcgga tcgagtcccc cgggtccgcg
     6661 aagtggaaca gcactccgtc gccgagcatc ttcacgacct ccccgtcgtg gcgtgaggcg
     6721 atctcgttca cgagctgggc gagcgagatc gagacgcggg ccgccacctc gtcgccggcc
     6781 tcctcggtga gctgcgtgta gccggagagg tccgcgaagg ccacggcttc cgcccccttc
     6841 ggcgaccgcg ggcggatgcc ggcctcctcg agcgccgtct ccacgtgctc gaactgatgc
     6901 tccgtcagga agcgctccga gtgtcgccgg aagagccagc ccagcatctc ctcgcccgaa
     6961 tgcccgatgc gcaggcccac ctctcggatc gcggcctcga acgcctggtt gtcgggcagc
     7021 ccccgggccc ggaacggctc ctcgatcgtg tggtggaagt agtgggtctg gaactgggcc
     7081 acgcggcggg cgctgtcgcc ccagacccgc accgcgcgga ggacctcgcc ctcccccacg
     7141 cccgccccga acagcacggg gagcgcccgg aggacgggga ggtcctcctc acgcacgagt
     7201 tcgtccgcag cggggcgcga gaggccgaag gccacgtagg ccgcctgcag cgtctcgagg
     7261 gggatgccga tctcctcgcc cagctcggcc aacgttcgat ccgaccgcgg gaaccgccgc
     7321 ccggcgctct ccaggtatcc cagcgtcagg tggccgccgg cgaccgccgc gcccagcgat
     7381 gccgggtcca tccccttcgc ctcgagctcc aggacgaccc gcacccgcat gacgtcccgc
     7441 cgggggacgg tcccgtcctc gggctccacg atcccgagct ccgcgagctc ccggaggtgc
     7501 tcgacgctga tccccgcgcg ctcggcgagc tcggcttcgc tgatccgatc cggggtcatg
     7561 cctcaggaca cgtacgactc gggatcctcc aagaacgcct cgtggcaggc ctcggagcag
     7621 aagtagtact tggtcccctc gtattcggcg gtcgccgccg cgtcctccgg gtcgatccgc
     7681 atcccgcaca cgggatcggt caccatggcc atgggagccc cccttcgtcg ggtcagtgga
     7741 ttctacgcgc cgccggcggc ggctagctcg cggcgcagcg tggggagtgg caccgagccc
     7801 atctcgagca gccggtcgtg gaagtcggcc aggcggaatc ccggccgccg ggacgcctcc
     7861 tcgcgcagcc gctcgatctc gagctgcccc accttgtagc agagcgcctg gcccggcatc
     7921 gcgatgtagc ggtccgcctc cgcctcggcc atccagcgcg cgagcccggt tccctccagc
     7981 tcggccacga cgcgctcgcg ctcccacccg aacgcgtgga cccccgtgtc gaccaccaga
     8041 cgggccgcgc ggagggcctg catctcgagc atgcccaggc gttcgtactc gtcgacgtag
     8101 agacccatct cgtcggccag gcgctcgctg tagagacccc acccctcgcc gaaagccgcg
     8161 ccctggagct cgttgcccag ccggcggatc gggtgtcgcc cctccgcctc cacggagagg
     8221 gccgtctcga agtgatggcc cggcgtggcc tcgtggtagg tggtgggcgc gaggctgtgc
     8281 agcggacggg gcgcggtgtt gacccagtag atcccggggc gggaccagtc gtcggtcggt
     8341 ccctggtagt agtcgagggc gtcctcctcg aggtccgggg gcacggctcg gacctcgcag
     8401 ttccgagcgg gcagccgacc gaagaacccc ttcgcggcct cccatccgcg ctccacctgc
     8461 tc
//
LOCUS       DASUTW010000007         3701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_132110, whole genome shotgun sequence.
ACCESSION   DASUTW010000007 DASUTW010000000
VERSION     DASUTW010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132110"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1178
                     /locus_tag="VF058_00185"
     CDS             132..1178
                     /locus_tag="VF058_00185"
                     /inference="COORDINATES: protein motif:HMM:NF021446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HEX6206754.1"
                     /translation="MVAIGLIVVLALVGLIVTAMRSGSSASIADGGPRVPARLSEELR
                     RWMEAGLLSRAQAEALAKFEAARVRGSRRGLIVEVVGYLGAVLAAAGAGVALGQAWGD
                     LRVWQRLATSFGTAGAFLVAGIVLSRLDEPAIRRLVSVLWTLSVASLAWGLIVLGVDV
                     LELAEERMSLFVGGVSLAYAGALWWVRRWPGQQVATFLALQVTVAGAILAPSGEAPAW
                     APMLAIWAVGAAWLVLGWRGLLAPSAMAPALGALAMVLAPTFGGGMDWNVLLGLGTSA
                     GLMVLSVPMRETLLLAIGSVGAFGYLVGTAVTYFGETLGVPLALLFAGLAVLAVAVLG
                     GRLLRLTRRPSGRP"
     gene            complement(1150..2103)
                     /locus_tag="VF058_00190"
     CDS             complement(1150..2103)
                     /locus_tag="VF058_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX6206755.1"
                     /translation="MGIARSALLWASTNRTLRRMAPRVWFVRRALRRFMPGETLEDGL
                     EAAETFRRLGIPTLFTHLGENVADQYEAIQATEDYLRAIDRVDELGLDTEMSVKLTHL
                     GLDLDPGLAAENYLRLVRRAAERGDTVWIDMESSEYVERTLEVYRTTLEASPNAGLCL
                     QAYLHRTRADLEDLLPLGPSIRLVKGAYREPPELALQRPDEIDEAFLGLSVTLLRHLQ
                     EGKVRRYAAGSHDLRLLGRVATASRDLGVSVKDWEIEMLYGIRHAEQLELARRGYPVR
                     TLISYGPAWYPWYVRRLAEKPGENLAYVLRNVFKAAPTASG"
     gene            2185..2334
                     /locus_tag="VF058_00195"
     CDS             2185..2334
                     /locus_tag="VF058_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEX6206756.1"
                     /translation="MAAIDPVCGMTVEESEAAATFEHEGRTYYFCSVDCKEEFEENPE
                     DYAEA"
     gene            2335..2688
                     /locus_tag="VF058_00200"
     CDS             2335..2688
                     /locus_tag="VF058_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX6206757.1"
                     /translation="MTALGAALVVFLFWTGSFLVLRWAGDVLNPPVTTAPSRVPFLGG
                     RGPETHAWSRFHVRYYAMALVFLAFDMEMVFMYPWAVVFLEEGLMAGIEMLMFITILV
                     LGILYGWREGAFRWA"
     gene            2679..>3701
                     /locus_tag="VF058_00205"
     CDS             2679..>3701
                     /locus_tag="VF058_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206758.1"
                     /translation="MGLRRALARVAAGAPTPVFVVAGVGGRRAAQHLDLSSVLAPTPS
                     PRAADALVVAGFLTERLLPAVMLAHDQIPAPRLAFQWLPDGAPDDPLGAALPGLRVVE
                     GGALPSIVRAEHERLLAGEGVRDDAWPDVEPSVWRGIGPYGQGGKGMTGGVPYGRPLP
                     GRADDRDGLKLDRLDVRVGPLFPGFPPGLELVLGVQGDVAQEVAVGENPFEGVVLGAR
                     TENVFARVAREAVPIAELEAARARSHLRWLADALRVAGLPSHGRRVLALAVRAGSGDV
                     QRVARLSRSLERGRGLRWHGGGVGVVREDVGGPVARAAGHPSDLRTELDEYRALGFEP
                     VVGEGGD"
BASE COUNT          428 a         1216 c         1465 g          592 t
ORIGIN      
        1 gcagatggag ggcgcggtcg gctgaggccg gcttcgccgt cccccctcct ctgaacgagc
       61 ctcctcccgg ggcggatgcc tcttgtcgtc tgctccccag gggagcatgg tgatcgggga
      121 ggtggcgacc gatggtggcg atcgggctga tcgtggtcct ggcgctggtc ggactcatcg
      181 tgacggcgat gcgctcggga tcgtcggcat cgatcgctga tggaggacct cgggtccctg
      241 cgcgcctctc ggaggagctc aggcggtgga tggaggccgg gctgctctcg cgggcccagg
      301 cggaggcact cgcgaagttc gaggccgctc gggtccgagg ctcgcgccgc ggcctgatcg
      361 tcgaggtcgt cggctacctc ggggccgtgc tcgcggcggc cggcgccggc gtggccctgg
      421 gtcaggcatg gggagacctc cgggtctggc agcgtctggc gacctcgttc gggaccgccg
      481 gagctttcct cgtggccggc atcgtgctct cgcgactcga cgagccggcc atccgccgct
      541 tggtgagcgt gctgtggacc ctctccgtgg cgtcgctggc gtggggcctc atcgtcctcg
      601 gcgtcgacgt gctggagctg gccgaggagc gcatgtcgct cttcgtaggg ggcgtgtcgc
      661 tcgcgtacgc cggcgccctc tggtgggtgc gacgctggcc cgggcagcag gtcgcgacct
      721 tcctggccct ccaggtcacc gtcgcgggag cgatcctggc gccgtccggt gaagcgcccg
      781 cttgggcccc gatgctcgcg atctgggcgg tgggtgccgc ctggctggtg ctcgggtggc
      841 ggggcctgct ggctccgagc gcgatggctc ccgccctcgg cgcgttggcc atggtgttgg
      901 ccccgacctt cggcggcggc atggactgga acgtcctgct gggcctgggg acatccgccg
      961 ggctcatggt gctgagcgtt ccgatgcgcg agaccctcct cctggcgatc gggtcggtgg
     1021 gggcgttcgg ctacctggtg gggacggccg tcacctactt cggggagacg ctcggcgtgc
     1081 cgctggccct gctgttcgcg ggcctcgccg tcctcgccgt cgcggtgctc ggcggacggc
     1141 tgctgcgcct cacccggagg ccgtcggggc ggccttgaag acgttgcgca ggacgtaggc
     1201 caggttctcg ccgggcttct cggcgagcct gcgcacgtac caggggtacc atgcgggccc
     1261 gtaggagatc agtgtgcgca cggggtagcc gcggcgagcc agctcgagct gctcggcgtg
     1321 gcggatcccg tacagcatct cgatctccca gtccttcacc gagacgccga gatcacgcga
     1381 ggcggtggcg acgcggccga gcaggcggag gtcgtggctc ccggcggcgt agcgacgcac
     1441 cttcccctcc tggagatgcc gaagcaacgt gaccgagagc ccgaggaacg cctcgtcgat
     1501 ctcgtcgggc cgctgaaggg ccagctcggg aggctcgcga taggcgcctt tcaccagccg
     1561 gatcgagggg cccaggggga ggaggtcctc gagatcggcg cgagtgcggt ggaggtaggc
     1621 ctggagacag agcccggcgt tcggcgaggc ctccagggtc gtgcgataga cctcgagcgt
     1681 ccgctcgacg tactcactcg actccatgtc gatccagacg gtgtcgcccc gctcggcggc
     1741 gcgtcgcacg agccgcaggt agttctcggc cgcgaggccc gggtccagat cgaggccgag
     1801 gtgcgtgagc ttcaccgaca tctctgtatc gagccccagc tcatcgaccc ggtcgatcgc
     1861 ccgaaggtag tcctcggtgg cctggatcgc ctcatactgg tcggccacgt tctccccgag
     1921 gtgggtgaag agggtgggga tgccgagccg gcggaaggtc tcggcggcct ccaggccgtc
     1981 ctccagcgtc tcgcccggca tgaaccgacg cagggcccgc cgcacgaacc atacgcgggg
     2041 cgccatccgg cggagcgtcc ggttcgtgga cgcccacagc agcgcgctcc tggcgatgcc
     2101 catcgcccgg gagggtagcc ggaacgacgg tcccgtgacg accccgggta tcgtgtgcgc
     2161 gagagacgac cgaaggagac tccgatggct gcgatcgacc cagtgtgcgg gatgacggtg
     2221 gaggagagcg aggcggcggc cacgttcgag cacgagggca ggacctacta cttctgcagc
     2281 gtggactgca aggaggagtt cgaggagaac cccgaggact acgcggaggc gtagatgacc
     2341 gcgctcggcg ccgccctcgt cgtcttcctc ttctggacgg ggagcttcct cgtccttcgg
     2401 tgggcggggg acgtcctgaa ccccccggtg acgacggcgc cgtcgcgcgt gccgttcctg
     2461 gggggccggg gccccgagac gcacgcttgg agccgcttcc acgtccgcta ctacgcgatg
     2521 gcgctcgtct tcctcgcctt cgacatggag atggtcttca tgtacccgtg ggccgtcgtc
     2581 ttcctggagg agggcctcat ggccgggatc gagatgctga tgttcatcac gatcctcgtg
     2641 ctggggatcc tgtacggctg gcgggagggg gcgttccggt gggcctgagg cgggccctgg
     2701 cgcgcgtggc ggcgggggcg ccgacgcccg tgttcgtcgt ggcgggagta ggggggcggc
     2761 gcgcggccca gcacctcgac ctgtcctcgg tcctggcgcc gacgccctcg ccccgggcgg
     2821 ccgacgcgct cgtcgtcgcc gggttcctga ccgagcgcct gctgcctgcg gtgatgctcg
     2881 cgcatgacca gatcccggcg cccaggcttg cgttccagtg gcttccggac ggcgcgccgg
     2941 acgatccgct gggggcggcg ctgcccgggt tgcgggtcgt ggagggcggc gcgctgccct
     3001 cgatcgtccg cgctgagcac gagcgcctcc tcgcgggtga gggcgtccga gacgatgcct
     3061 ggcccgacgt cgagccctcc gtctggcggg ggatcggccc gtacgggcag ggcgggaagg
     3121 ggatgactgg gggcgtgcct tacggacgtc ctctgccggg gcgggccgac gaccgggacg
     3181 ggctgaagct cgaccgcctc gacgtgcggg tgggcccgct gttcccgggg ttccctcccg
     3241 ggctggagct cgtcctgggc gtgcagggcg acgtggccca ggaggtggct gtcggagaga
     3301 acccgttcga gggggtcgtg ctcggcgccc gcaccgagaa cgtctttgcg cgggtcgcgc
     3361 gagaggcggt cccgatcgcg gagctggagg ccgcgcgggc ccggagccac ctccggtggc
     3421 tggcggacgc gttgcgggta gcgggactcc cgtcgcatgg acgtcgggtc ctcgccctgg
     3481 cggtccgggc gggatcaggg gacgtccagc gggtcgcgcg gctgagccgg tcgctggagc
     3541 ggggccgcgg gctcaggtgg cacgggggcg gggtgggggt ggtgcgcgag gacgtcgggg
     3601 gaccggtcgc ccgcgcggcg ggtcatccga gcgacctccg gacggagctg gacgagtacc
     3661 gcgccctggg cttcgagccg gtggtcgggg agggcggcga c
//
LOCUS       DASUTW010000008         6500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1816478, whole genome shotgun sequence.
ACCESSION   DASUTW010000008 DASUTW010000000
VERSION     DASUTW010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816478"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VF058_00210"
     CDS             complement(<1..486)
                     /locus_tag="VF058_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX6206759.1"
                     /translation="MKAVIMAGGEGTRLRPLTSNQPKPMVPIVGRPMMEHVLHLLERH
                     GFDESVVTVQFLASVIRNYFGDGDDMGIDLAYVTENEPLGTAGSVKNAEHFLDETFLV
                     ISGDAVTDIDLTEAVAFHREKGSAVTVVLKRVENPLEFGIVIADDEGRIERFLEKPGW
                     GE"
     gene            complement(522..1118)
                     /locus_tag="VF058_00215"
     CDS             complement(522..1118)
                     /locus_tag="VF058_00215"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX6206760.1"
                     /translation="MDGGGVDGDTRILTVPNVLSFVRIAAIPLFVVLLLDEGARLWGF
                     LVLGVVQGTDWVDGFVARRYGQVTELGKVLDPLADRLAVGAALITFVLADLFPLWAAL
                     LVLVRDGLVLVAVGVLALIGAPRIEVRRIGKIATFVLMWAIPMIAWGNAGFLLDDVML
                     VHGWIWFGVGYLEYLVATGAYVVDVREALRGRPPSLEG"
     gene            1176..1670
                     /locus_tag="VF058_00220"
     CDS             1176..1670
                     /locus_tag="VF058_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206761.1"
                     /translation="MEKRLLSIYLADHLAGSRGAVELARRSMKSNEGSPLGDDLMRVV
                     REIEEDRATLEELMEMAGVGRAPVKDGAVWLVERLARLKLNGRLLAYSPLSRLEELEV
                     LRLGIEGKRSLWRNLSEIRAEVEGWEGVDFAALIERAEGQIDVLESHRIATVRQAFFQ
                     PTTV"
     gene            1680..2813
                     /locus_tag="VF058_00225"
     CDS             1680..2813
                     /locus_tag="VF058_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6206762.1"
                     /translation="MAIETPPADPLLRASQDGEGGDRLRIAMVAPPWYSIPPSGYGGI
                     ESLVSSLTERLVQRDHDVTLIAAAPSKTSATFRATLAEPPSHRMGEALPEIVHTARAR
                     TILEEIEPDVVHDHSTAGPLLAGFGAPTILTAHGPVRGDFGAYYRSVAHRVGLVAISD
                     AQRELAPEIPWFDMVHNAIPVRDYTFRDTKEDFALFLGRMSPEKAPHLAIEACREAGR
                     RLLMAAKCSEPDEIEYFEAEVEPRLGPDTEWLGEVDDETKKDLLSRARCLVFPIQWDE
                     PFGLVMAEALASGTPVVACRRGSVPEVVGDGVTGFVCDELDQLPDAIRRAGELDPGAC
                     RERASSMFDLDVMVSGYERVYRKAIGEADDPPVTRPRRGANRS"
     gene            complement(2779..3318)
                     /locus_tag="VF058_00230"
     CDS             complement(2779..3318)
                     /locus_tag="VF058_00230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6206763.1"
                     /translation="MVAVRSAPTNGLGPEEVRAIRELLVAAWETEPDDFGDEDWEHAV
                     GGTHFILEDGGEIVAHASVVERELHTGGHELATGYVEAVGTHPEHRRRGHASALLREV
                     ADHIDARYQLGALGGDPAFYVPLGWRVWEGPTFVRTEEGLVRTPEEDGSVLVRPTPST
                     PPLDLSAPISCDWRPGEVW"
     gene            3411..3641
                     /locus_tag="VF058_00235"
     CDS             3411..3641
                     /locus_tag="VF058_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206764.1"
                     /translation="MQIVNGDMSGNSCAIARRENTRWRFSRFRQERTWASCRFRARYV
                     HPNKMIHWRVVSTTIEAERRALDCAPDAGWYP"
     gene            3847..4164
                     /locus_tag="VF058_00240"
     CDS             3847..4164
                     /locus_tag="VF058_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206765.1"
                     /translation="MDSDAIIAIAAVIQLVILLVFLVMAANVGKIRQLLEKPVTLRRQ
                     CPHCLETMRRDAAVCPHCQRESDPWQLRDGHWWSTRDGRSYRYNEQNGEWVEASSSAE
                     AQP"
     gene            complement(4321..4394)
                     /locus_tag="VF058_00245"
     tRNA            complement(4321..4394)
                     /locus_tag="VF058_00245"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4358..4360),aa:Pro,seq:ggg)
     gene            complement(4417..5742)
                     /gene="der"
                     /locus_tag="VF058_00250"
     CDS             complement(4417..5742)
                     /gene="der"
                     /locus_tag="VF058_00250"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX6206766.1"
                     /translation="MSDPHRIAGRPPRVAIVGRQNVGKSTLLNRLLGAREAIAHETPG
                     VTRDRLEVEVSWRGRRFVAIDTGGYVRRATGIESLVAEQVERALEQADLVLLVVDAVT
                     GPQDEDLDLARRLRRAEVPVVLVANKVDSETQEPEASRLYSLGLGEPMAVSAVHGRGS
                     GELLDRIVDLLPEAPEGEPEPIEERAFAIVGRPNVGKSSLFNRIVGERRAVVSPVAGT
                     TRDAVDSLVTWDGEPLRFVDTAGFRKPSRAESLEYYGFTRAVKAIERSHVACLVIDAS
                     EGVTTEDRKIAARVSELGRGLVVVANKWDLVEEERDRRFREIRERVAVFRGVSVLRTS
                     AHTGVGVGKVVPALLAVHREWTRRVPTSKVNEILLSAQAETPPPRAGGRIKYATQIAA
                     GPPRFVLFTTGRIPTTYARFIENRLRDAFGFEGTPIRLIFRRRQRRGAR"
BASE COUNT          999 a         2344 c         2269 g          888 t
ORIGIN      
        1 ctcgccccag cccggcttct ccaggaagcg ctcgatccgg ccctcgtcgt cggcgatcac
       61 gatcccgaac tccagggggt tctccacgcg cttcagcacc acggtcacgg ccgagccctt
      121 ctcgcggtgg aaggccacgg cttccgtgag gtcgatgtcc gtgacggcat cgccggagat
      181 cacgaggaag gtctcgtcga ggaagtgctc cgcgttcttg acgctgccgg ccgtccccag
      241 cggctcgttc tcggtcacat aggcgaggtc gatccccatg tcgtccccgt cgccgaagta
      301 gttgcggatc acgctggcca ggaactgcac ggtcacgacc gactcgtcga agccgtgccg
      361 ctcgaggagg tggagcacgt gctccatcat ggggcgcccc acgatgggga ccatcggctt
      421 gggctggttg ctggtgagcg gacggagccg ggtgccctcg ccgcccgcca tgatgacggc
      481 cttcatcgcg ccatgatacc cacgggcccg cggcccacgg gtcagccctc gagcgagggc
      541 ggtcgccccc gcagcgcctc ccggacgtcg accacgtagg cgccggtggc cacgaggtac
      601 tcgaggtagc ccaccccgaa ccagatccac ccgtggacga gcatcacgtc gtcgaggagg
      661 aaaccggcgt tcccccaggc gatcatgggg atggcccaca tcagcacgaa ggtggcgatc
      721 ttcccgatcc ggcgcacctc gatccggggc gcgccgatga gagccagcac gccgaccgcg
      781 acgaggacga gcccgtcgcg cacgagcacg aggagcgccg cccacagcgg gaacaggtcc
      841 gccaggacga acgtgatcag cgccgccccc accgccagcc ggtcagcgag cgggtccagg
      901 accttcccca gctcggtcac ctgtccgtac cggcgagcga cgaacccgtc cacccagtcg
      961 gtcccctgga ccacccccaa cacgaggaag ccccagagcc tcgccccctc gtccaggagc
     1021 agcaccacga acagggggat cgcggcgatg cggacgaacg agaggacgtt cgggacggtg
     1081 aggatgcgcg tgtcgccgtc gactcccccc ccgtccacga ggagagtcta gcccccgcgg
     1141 attggggcgg cgccggatgg ggaaggaccc gcggcatgga gaagcggctg ctctcgatct
     1201 acctcgccga ccacctcgcc ggctcccgcg gcgccgtgga gctggcccgg cgaagcatga
     1261 agagcaacga gggctctccc ctgggcgacg acctgatgcg cgtggtgcgg gagatcgagg
     1321 aggaccgggc gacgctcgaa gagctgatgg agatggccgg cgtggggcgc gcgccggtga
     1381 aggacggcgc cgtctggctg gtggagcgcc tggcccgcct caagctgaac gggcggctcc
     1441 tggcctactc gcccctcagc aggctcgagg agctcgaggt ccttcgcctg gggatcgagg
     1501 ggaagcgctc gctgtggagg aacctctcgg agatccgcgc cgaggtcgaa gggtgggagg
     1561 gcgtggactt cgcggcgttg atcgagcggg cggagggaca gatcgacgtg ctggagagcc
     1621 accgcatcgc gacggtgcgg caggccttct tccagcccac aacggtctga gacgagccga
     1681 tggcgataga gactccccct gccgacccgc tgctccgtgc gtcgcaggac ggggagggtg
     1741 gggaccgtct ccgcatcgcc atggtcgcgc ccccctggta cagcatcccg cccagcggct
     1801 acggcgggat cgagtcgctc gtgtcctcgc tgaccgagcg gctggtccag cgggaccacg
     1861 acgtcacgct catcgcggcc gccccctcga agacgagcgc gacgttccgt gccacgctgg
     1921 cggagcctcc gagccaccgg atgggagagg ccctgcccga gatcgtccac accgcgaggg
     1981 cgcggacaat cctcgaggag atcgaacccg acgtggtcca cgaccattcg accgccgggc
     2041 ccctcctggc gggattcggc gctcccacca tcctcacggc ccacggcccc gtgcgaggcg
     2101 acttcggcgc ctactaccgc tcggtggccc accgggtggg actcgtcgcg atctccgatg
     2161 cgcagcgcga gctcgccccg gagatcccgt ggttcgacat ggtccacaac gccatccccg
     2221 tccgcgacta cacgttccgc gacacgaagg aggacttcgc cctcttcctg ggccgcatgt
     2281 cgccggagaa ggccccgcac ctcgcgatcg aggcgtgccg cgaggccggc cgacgcctcc
     2341 tgatggccgc gaagtgcagc gagccggacg agatcgagta cttcgaggcg gaggtcgaac
     2401 ctcgtctcgg gcccgacacc gagtggctcg gggaggtcga cgacgagacg aagaaggacc
     2461 tgctctcccg ggcgcgatgt ctcgtcttcc ccatccagtg ggacgagccg ttcggtctcg
     2521 tgatggccga ggcgctggcc agcggcaccc ccgtggtggc ctgcaggcga ggatcggtcc
     2581 ccgaggtcgt gggcgacggc gtcaccgggt tcgtctgcga cgagctcgat cagctgccgg
     2641 acgcgatccg gcgagcgggc gagctggatc cgggcgcgtg ccgggagcga gcctcctcga
     2701 tgttcgacct ggacgtgatg gtctcgggct acgagcgcgt ctaccggaag gccatcgggg
     2761 aggccgacga tcccccggtc accagacctc gccggggcgc caatcgcagc tgatcggcgc
     2821 ggagaggtcg agcggcggcg tcgagggcgt cgggcgcacg agcacgctcc catcctcctc
     2881 gggggtgcgc acgagcccct cctcggttcg aacgaacgtg ggaccctccc agaccctcca
     2941 tcccaggggc acgtagaagg cgggatcgcc tcccagggcc cccagctggt agcgagcgtc
     3001 gatgtggtcg gccacctctc gcagcagggc cgacgcatgg ccccggcggc ggtgctcggg
     3061 gtgcgtcccc acggcctcga cgtagcccgt ggccagctcg tggcccccgg tgtggagctc
     3121 ccgctcgacg accgaagcgt gggccacgat ctcgcctccg tcctcgagga tgaagtgcgt
     3181 cccgcccacg gcgtgctccc agtcctcgtc cccgaagtcg tcgggctcgg tctcccaggc
     3241 ggcgaccagc agctcgcgga tcgcccggac ctcctccggc ccgaggccgt tcgtcggtgc
     3301 gctcctcacc gcaaccatgc gggccaccat accgaccgag gaagcgcggc gaaggacccg
     3361 cgcacaacgt ctacatcgac tcccggggag ggccgagaca cgattacgtg atgcagatcg
     3421 tcaacggcga catgagcggc aactcttgcg ccatcgcccg ccgcgagaat accaggtggc
     3481 gcttcagcag attccggcag gagaggacgt gggcatcgtg ccggttccgc gcccgctacg
     3541 tccaccccaa caagatgatc cactggaggg tggtgtcaac aactatcgag gccgagcgga
     3601 gggccctgga ctgcgctccg gacgcgggat ggtacccgta gggtggatgc ggtcgtcgcc
     3661 tcgcccgacc gcgacgaccg gagtggggcg cgagatggcc gcccagcggt ttgggcgtcc
     3721 gcaacgcgat caagggggag cccctccggt ccccaatgaa tccagttggc aatcggctcg
     3781 ccgctcgacc cgcgtggaat ggcccgatag agtgacgtga ggcgagcaag cagaagggag
     3841 gcccccatgg actccgatgc catcatcgcc atcgccgcgg tcatccagct cgtcatcctc
     3901 ctcgtgttcc ttgtcatggc ggcgaacgtg gggaagatcc gccagctact ggagaagccc
     3961 gtcaccctga ggcggcagtg tccccactgc ctagagacca tgcgtcgcga cgcggccgtg
     4021 tgtccccact gccagcggga gagcgatccg tggcagcttc gggacgggca ttggtggagc
     4081 accagggacg ggcgatccta ccggtacaac gagcagaacg gcgaatgggt cgaggcctcg
     4141 tcgtcggccg aggcccagcc ctaaccggca tccactctcc gcgctggcct agccggtgga
     4201 cgcgaatctc cgttcggtgt gcggggcgcg tttgtgcctc tcctctgcct ggagtcgaac
     4261 gctggttccc gcgtctctcc gcggacacag agaagggcct ctgaggaggc ccttctctcg
     4321 tcgggccggc cggatttgaa ccggcgacct cttgaccccc agtcaagcgc gctgccaagc
     4381 tgcgccacgg cccgtgggcc ctagtgtacc gctcgatcag cgagcgccgc gacgctggcg
     4441 gcggcggaag ataaggcgga tcggcgtccc ctcgaacccg aacgcgtcgc ggaggcggtt
     4501 ctcgatgaac cgcgcgtagg tggtcgggat gcgcccggtg gtgaacagca cgaaacgggg
     4561 agggccggcg gcgatctggg tcgcgtactt gatgcggcct cccgcccgcg ggggcggggt
     4621 ctcggcctgg gccgagagca ggatctcgtt caccttcgag gtggggaccc ggcgcgtcca
     4681 ttccctgtgc acggccagca gcgcggggac gaccttccca acccccacgc ccgtgtgcgc
     4741 ggaggtccgc aggaccgaga cgccccggaa gaccgccacg cgctcccgga tctcccggaa
     4801 ccggcggtct cgctcctcct ccacgaggtc ccacttgttc gccaccacga cgaggccccg
     4861 gcccagctcc gagacgcggg ccgcgatctt ccggtcctcg gtcgtcaccc cttccgaggc
     4921 gtcgatcacg aggcacgcca cgtggctccg ctcgatcgcc ttcacggccc gggtgaaccc
     4981 gtagtactcg agggactcag cgcgagaggg cttgcggaac ccggcggtgt cgacgaagcg
     5041 gagcggctcg ccgtcccagg tgacgagcga gtccacagcg tcccgcgtgg tgccggccac
     5101 gggcgagacc accgctcgcc gctcccccac gatccgattg aacagcgacg acttcccgac
     5161 gttggggcgg cccacgatgg cgaaggcgcg ctcctcgatc ggctcgggct cgccctcggg
     5221 ggcctcgggc aggaggtcca cgatgcggtc gaggagctcg ccggagccgc ggccgtggac
     5281 cgcgctgacg gccatcggct cccccaaccc caacgaatac agacgcgagg cctcgggctc
     5341 ctgcgtctcg gagtcgacct tgttcgccac gagcacgacg ggtacctcgg cgcggcggag
     5401 gcggcgcgcc agatcgaggt cctcgtcctg gggcccggtg accgcgtcca cgacgagcag
     5461 gacgaggtcg gcctgctcca gagcccgctc cacctgctcg gcgaccagcg actcgatccc
     5521 cgtggcgcga cgcacgtacc ccccggtatc gatcgccacg aagcgccggc cccgccacga
     5581 gacctcgacc tcgagccggt cgcgggtgac cccgggggtc tcgtgggcga tcgcctcgcg
     5641 ggccccgagc aggcggttga gcaaagtcga cttccccacg ttctggcggc cgacgatggc
     5701 cacgcgaggt gggcgtccgg cgatgcgatg ggggtcgctc accccccttt cctccctcgc
     5761 ccaggcgctc gcggacgagc gacaggaccg cctcgaagac ctcatccgcc gatcgcccgc
     5821 tcgagtcgac gacgacggcg tcgggggcgg ggacgaaggg gctgacccgg gcgtcgagcg
     5881 cgtcgcgcgc cgcgaccgcc gccgccagct ccgggtcgcc gagctcctcc tcgcggcgcg
     5941 cggcgcgctc ctcgaggtcg gcgtggagga agaccttgac cgacgcctcg gggaagacca
     6001 ccgtcccgat gtcgcgcccc tcgaccaccg ctcccccacc tccgaggcgg cgctggacct
     6061 cgtggagggc ggagcggagc tccgggtgcc gggacacctg ggacacgatc gactccacct
     6121 cgggcgtgcg cagctcgtcg ccgggagggg ccccgtccac gaggagcgac ggcggggtcg
     6181 cggcctcgtc catctcgaac gagagatccc ccaccagcgc ggccagggcc ggtccgtccg
     6241 aggggtcggt ccccgtccgg agcgcggctc gggccaccgc ccggtaggtc gtgcccgtgt
     6301 tgaggtacgg cagtttcagc gccagagcca ggcggcgggc cagcgtgctc ttaccggagc
     6361 cggccgggcc gtcgatcgcc accacggggc cgctcaccct tccacctcca cgtcggcccc
     6421 gagcgcgcgg agggcgtcca cgaagcccgg gaacgagacg gcggcccact ggacgtcgtg
     6481 gatcacgctc tcgcgctcgg
//
LOCUS       DASUTW010000009        27947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_261981, whole genome shotgun sequence.
ACCESSION   DASUTW010000009 DASUTW010000000
VERSION     DASUTW010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 27947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27947
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261981"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VF058_00255"
     CDS             complement(<1..384)
                     /locus_tag="VF058_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX6206767.1"
                     /translation="MRSDITVGGTFPDYELTDHTGTRRSLSELQGDDPMILVLSRGHF
                     CPKDRRQHRNLVDLYPELKVGYTRIVTISTDNLLETNELRDGLGAQWAFLSDPGRKVQ
                     QDLDIQEYTDPQNDPMIPYTFVLGPG"
     gene            596..2440
                     /locus_tag="VF058_00260"
     CDS             596..2440
                     /locus_tag="VF058_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019547755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX6206768.1"
                     /translation="MSAIDDYALLSDGHGSALVSKEGSIDWACLPRFDSPSVFGRILG
                     DGAGHWRIAPSADFETERAYLDDTMVLRTTFRSGGGAVALTDTMPFERAEGEHRIGHR
                     APQAVVRSIEGIEGEVEMELELAPRPEYGLTTPTLVPVEGGVGSRGGPQAYVVSSPVP
                     LHLEEGVARGSCTVRAGERLAFAIRVGSPWDGMGRPWGQEEIVRRLDETIDAWVAWSA
                     EHEGYQGPYREIVQHSGRVLQALTYVPTGAVIAAPTTSLPETIGGSRNWDYRYCWVRD
                     ASLTLKALWVAACPDEAQQFFDFFVVAAGGHLHEGRELQVVYGIGGERDLAERELTHL
                     DGFRGSRPVRVGNRAWEQFQLDIYGALLAAAHLLAERMGGFDDATGHFLCDLANIAAA
                     RWEEPDQGIWEVRGDPQHFLHSKLMCWVALDRAVRLAPMIGGERDVERWSEIRDRIRA
                     AILERGWSDRAGAYTQAFGSDELDASALMMPIVGFLPADDSRMRATIHAVSSGLTDER
                     GLVYRYKAADGLPGEEGVFGICTYWMVQCLAMLGEVDRAKELFETMTSFGNDVGLFSE
                     EVDPGSGEALGNFPQAFTHIGLVNAAWVIAEAERGRSSRSDDQAKGQA"
     gene            complement(2419..3057)
                     /locus_tag="VF058_00265"
     CDS             complement(2419..3057)
                     /locus_tag="VF058_00265"
                     /inference="COORDINATES: protein motif:HMM:NF042610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HEX6206769.1"
                     /translation="MDPALDTERRVRPNLVHSGTPETRSTHHPVWGPIGLMLSMAVPI
                     AVIVVGVAVEGSAAISWQGAIVWGVVATVAFTILGMVGTAMGMTRMDLLDLLGSAVAT
                     PHTTGSRVIGAVIHHMNGAVLAVAFVYAGTLVGLSPDWIAGLVWGAILWALALLMMTT
                     IGSVHPAIRRGQQEDPGPAGVRFGAMTPVGSLMGHLVYGALLGLLYQAWPLA"
     gene            3220..3813
                     /locus_tag="VF058_00270"
     CDS             3220..3813
                     /locus_tag="VF058_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206770.1"
                     /translation="MIAVPGVWSVPLVGATSLLVVVGAVLVSGIARGRIAMELGWGRS
                     RMSLRDRRVEIAAPPDVVLHLLRQAAAGEVPGVTERERTEVIDADGDLIVNESLNDSR
                     FGMVRAREAVRFHPDGRVTYRHLAGPLPGTEEAFEVKSADGGSVLTYHGGIPVDFWAL
                     GRLVARFLILPEYERLLDEHLAALTKTAEARARRRRT"
     gene            complement(3815..4633)
                     /locus_tag="VF058_00275"
     CDS             complement(3815..4633)
                     /locus_tag="VF058_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6206771.1"
                     /translation="MSSRTSEGQRAGPPILDPAFLKTCCADLWSHPGVRVLLGDHLHP
                     GARALTERTLDLLALPEEATLLDVGSGPGAGAGVAAERGCRVVAVDLSTSAVTEAAEA
                     PGVAGVAADAERLPLSDEALDGVLAECVLSAIPGKEAVAREVHRVLRPGSPFVLTDVT
                     RQGSLPADLDSLMGWIACAGGALPLQGYARLLEDAGFTVERLEDHREALGEFLAQVRR
                     RLALFQGALAAGVVNPAEMGIPTELLGLGQRLLEIAAAASADGSLSYALILARA"
     gene            complement(4630..6018)
                     /locus_tag="VF058_00280"
     CDS             complement(4630..6018)
                     /locus_tag="VF058_00280"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX6206772.1"
                     /translation="MNGTTSLCPICLRPSSATHVERDNRVYLRRTCPEHGTAEALVSS
                     DAGWYEDSRRFLRPGRKPAHAATESEIGCPYDCGFCPEHEQHACVTVLEITEACNLEC
                     PACFAGEAHAKHASLEDVEAMTGAVLAAEGGEADVVMLSGGEPTIHPRFFEVADLVRS
                     KPIRYVLVNSNGIRIARDPGFARGIAEREMLVYLQFDGFTEDVYRTLRGRGDLLEVKH
                     RALENLAVAGARVVLVATVLAGVNDDQVGELVRFGATHPAVRAVSLQPEFGEGRYVPF
                     DPMDRMDVAAVVKKVAEGSGIFTREDFIPIPCCDPICTAAAYAYVSDGSVTPVTRLVP
                     VDTYLDYVANTSMPNLSQAFRQDAEEMREALLRLFSKSNPAGSPGQAEAFACACGPLL
                     AEIDAVDDLHDRMFAVTIEAFMDRFTFDVNRAKRCCIQEALPDGRIVPFCVYNTLYRF
                     APDHRPVPPEGS"
     gene            complement(6035..6307)
                     /locus_tag="VF058_00285"
     CDS             complement(6035..6307)
                     /locus_tag="VF058_00285"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX6206773.1"
                     /translation="MGLFKTAATCTHLPEGVVEPRSETCEACGSGYNLRACAHCGFVG
                     CCESQAGHNREHALTARHPVIRSLPLGRDAFTWCYECRRYVDASDL"
     gene            6479..7153
                     /locus_tag="VF058_00290"
     CDS             6479..7153
                     /locus_tag="VF058_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX6206774.1"
                     /translation="MTGMFRQAAWLARSLGRGDLAPFTDDDIRELAESIGLRTVEAGT
                     PLLEQGKPVAFIAVIQRGDVGLYYRSGLRRVMLQRLHEGDVLGDVPYFCRTGSPFSAR
                     SLSEVDLLHLEDEVLRRLLYTRPAVTQRFLYSLATRLERMQRRLLEITQRDLRGQVAT
                     LLLNETEEQPGEIHLPQSTLAELLGATRPSVNQVLKGFEREGLVRLSYRRLEVVDPDG
                     LRRAIS"
     gene            complement(7183..7416)
                     /locus_tag="VF058_00295"
     CDS             complement(7183..7416)
                     /locus_tag="VF058_00295"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6206775.1"
                     /translation="MKPSCEEVLRDIEVYLDGELPNPEAAALAEHLGACPECLEHEAF
                     ISRLRATLRTKLGASSEVPDSLVDRIRRTIATS"
     gene            complement(7413..8012)
                     /locus_tag="VF058_00300"
     CDS             complement(7413..8012)
                     /locus_tag="VF058_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018023469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6206776.1"
                     /translation="MSGQRVALVRTDASQDGLKRRFEEEAIPLLKTLYPAAMSLTRNP
                     ADAEDLLQETYLRAYRGFAGFREGTNLKAWLYRILTNTYINIYRKRRREPEILSSDEA
                     PDWYLYDKIRESDLAPSAEVQALERMPDAEVQDALDSLPDRFRMVVLLADVEGFLYRE
                     IADILDIPRGTVMSRLHRGRNALRKALWESMERRGVTGG"
     gene            8198..10246
                     /gene="tkt"
                     /locus_tag="VF058_00305"
     CDS             8198..10246
                     /gene="tkt"
                     /locus_tag="VF058_00305"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX6206777.1"
                     /translation="MQAREDDLRPDVVVHTHEGGPPRGTDLDILSVNAIRALAMDAVE
                     AAGSGHPGTPMALAPVAYVLWTRFLRHDPHDPEWPDRDRFVLSAGHASMLLYSVLHLS
                     GYELPLDELRHFRQWGSRAPGHPERGITPGVEVTTGPLGQGFANAVGMAIAERMLADR
                     FNRPGHDVVDHRTYGICSDGDLMEGVSAEAASLAGDLRLGKLTMLYDDNHITIEGSTD
                     LAFCEQVSERFRAYGWHVLQVSDANDLEEVGAALDTAGAESERPSLIVVRSVIGYGAP
                     TKQGTAAAHGSPLGPDELRAAKERLGWRYPEPFTVPEEVLAHFRDVLDEGVSPHADWR
                     ERFARYQEEHPGLAAEFWRVLSGELPSGWDDAMPTFEVGAEVATRSASGSVLNAIASA
                     VPELVGGSADLAPSTDTYLEGFADVSCADFSGRNFHFGVREHAMGAVMNGMAAHGGFR
                     PYGGTFFVFSDYMRPAIRMAALMGLPVTFVLTHDSIGLGEDGPTHQPVEHLAGLRAIP
                     DLVVIRPADANETAQAWRVTMERREGPVALVLTRQKLAVLPSLRTDAVARGAHVVADG
                     EALALVASGSEVHLALAARTLLASSSISARVVSMPSWELFEAQSEGYRDEVLPPGLPT
                     LAIEAASGFGWRRWADDSVSLDRFGASAPAEVLFREFGFTPEQVAERARRLLRKGHNG
                     "
     gene            10243..11142
                     /locus_tag="VF058_00310"
     CDS             10243..11142
                     /locus_tag="VF058_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX6206778.1"
                     /translation="MILLAVDLGGTRMRAALVADDGRIVERRDRPTPRERSCAGALLE
                     LAGGVVEGSGVREAVIGVPGRVDYGAGRMEHAPNLPAHWAPELTEGHLSSALGLRVAL
                     ANDADLAAVGEFRFGAGADARDVVYLTISTGVGAGVILGGRLVRGRRSLAEAGHTIID
                     RSSLKRGEPATFEDQASGTALARLGRAVGIDDGAAAVLEATVAGDASAARVWNQVVEA
                     AAVGATNLAHLFSPEVIVVGGGVANAGALLLDPIRRHLDEHGPSSLPSPIRVVGARLG
                     DDAALAGAPAWREIALGEVGARA"
     gene            11135..11773
                     /locus_tag="VF058_00315"
     CDS             11135..11773
                     /locus_tag="VF058_00315"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6206779.1"
                     /translation="MPSRSPHVVLVRHGETEWTARGRHTGRTDVPLTERGREEAGMLA
                     ERLRSWSFGRVLASPLSRALETCRLAGYGEGAEIRDELMEWDYGRYEGRTTAEIRKKV
                     AGWSIWSQGAPDGEAAEDVGGRIDPIVQELREAEHDVAVFGHGHLLRALAARWLELPP
                     AEGRRFALDTTTVSVVGHERETPALLRWNEDCHLGPRGDALLTPATGEDTGA"
     gene            11770..14406
                     /locus_tag="VF058_00320"
     CDS             11770..14406
                     /locus_tag="VF058_00320"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013060583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HEX6206780.1"
                     /translation="MNPLRQLREAGQSVWLDYLRRGLIAGGGLQELIDEDGVGGVTSN
                     PSIFRKAIGGSTDYEAAIAALAGNGTNARDAFYRLALEDIRSACDVFLPTWEETEGLH
                     GFVSFELEPGLARDTKGSIVAAADLAARIDRPNVMIKVPGTEEGVPVVEELTAAGLNV
                     NITLLFSVDRHEEVARAYVAGLRRGLEAGQQIEGIASVASFFVSRVDSKVDARLPDDS
                     PLRGRIAIANAKMAYRRFQEIFSGEEWEELAAAGGRVQRPLWASTSTKDPELRDVLYV
                     ESLVGPQTVNTMPQATMDAFRDHGRVDPAAVSDGLDEAEDALARLEEAGVSLESVTSD
                     LVEEGIAAFAADFEALLETIEGTLARSRAGESQRGSSLGALDGPVRARLDALQERSIV
                     RRIWRKDHTVWKDDPTEIVDRLGWLTVADRMHERIEELEAFAKESVADGFETAVLLGM
                     GGSSLAPEMLMRTFGATGDRLELVVLDTTHPVAIARIETELDLARTLFLVASKSGATV
                     ETLSHLDHFWERTRGAAERFVAITDPGTPLAELARERGFRKVWINPEDIGGRYSALSL
                     FGLVPGALIGAPLRELLDGAEEMGIACDACIESKDNPAGWLGAVLGDASLSGRDKLTL
                     VLPEEIASLGSWVEQLIAESTGKEGTGIVPVVGEDLGAPDVYGRDRLFVAYGDHEGLE
                     AIEAAGHPVVRLPYDGPTSLGRELFRWEFATAVAGHVLGINPFDQPNVAEAKQATAEI
                     LESGIEEPGLDDLPPLLEQVRPGDYLSLHAYLDPTDETAAVLHRARMALRDRYAVATT
                     VGFGPRFLHSTGQLHKGGPATGVFIQVVDPDRPRDVEIPGKPYSFGRLIDAQALGDLR
                     SLRGRGRRVARVTLEQLLGGVA"
     gene            14406..15281
                     /gene="gnd"
                     /locus_tag="VF058_00325"
     CDS             14406..15281
                     /gene="gnd"
                     /locus_tag="VF058_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007502501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX6206781.1"
                     /translation="MQIGIIGLGRMGGNMAERLRRGGHEVVGYDRSGERSDVGSLEEL
                     AGKLDAARTVWTMIPAGEPTEQAVRELAGLLEPGDVVVDGGNSNFRDSMRRARELEER
                     GILFVDVGVSGGVWGLEEGYGLMVGGTDEAVARLVPILDVLAPEGRYVHVGPPGSGHF
                     AKMVHNGIEYGMIQAYAEGFDILQASREFPDIDLHAVAEAWRHGTVIRSWLLDLAADA
                     LEKDPRLEDLEAYAEDSGEGRWTVLEAIDTAVPVPVIAAALFARFSSREESPFSMRMV
                     AALRREFGGHAVKER"
     gene            15278..16726
                     /gene="zwf"
                     /locus_tag="VF058_00330"
     CDS             15278..16726
                     /gene="zwf"
                     /locus_tag="VF058_00330"
                     /inference="COORDINATES: protein motif:HMM:NF009492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX6206782.1"
                     /translation="MSADEVRPQAVVIFGASGDLTRRKILPALYNLAREGLLPERYAV
                     VGYARTEWDDEAFRAHARRAIQDFSRTPVDEDVWKRFSDAISYVAGPFDDPGCFAPLR
                     WRLEDLDEARGTEGRRLYYCATPPSAFPEIVRRLGEAGIGEDARIVIEKPFGHDLESA
                     RSLNRTVHDVFHESAVFRIDHYLGKETVQNLVVLRFANSLFERVWNRDAVDHVQITVA
                     EQVGLEGRGAYYEEAGALRDIVQNHVLQMLALLAMEPPRSLEPDAIRDEKVKLLNALR
                     PLDPGGVVRGQYASGTSDGEVVPGYLEEEGVAADSQTETYAALRVIVDNWRWEGVPFY
                     LRTGKRLPRRVTEVTVVLRRAPAYLFAELGMSPLAPDHLTFRIQPDEGISLAFQAKEP
                     GPGIAPKTVRMEFDYEGSFEHTPAEAYERLLLDAMAGDQTLFTREDGVERAWRVVMPA
                     LERPGPIHAYPAGSWGPKAADELIAPRSWHLH"
     gene            16892..17335
                     /locus_tag="VF058_00335"
     CDS             16892..17335
                     /locus_tag="VF058_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206783.1"
                     /translation="MVLHGILAAFIGTGLMTISSTTEMQWAGREASVAPGLATAKLLR
                     PFGVREVKGRALDLLATWTHWVYGTLWGLALWALVGPAGLPLWAAGIAFFFLVWGAEQ
                     IHLPVLGIAPWPWKWGLKANLTDMWHHIVYAAGATGAWALIDVAG"
     gene            17340..18494
                     /locus_tag="VF058_00340"
     CDS             17340..18494
                     /locus_tag="VF058_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing protein"
                     /protein_id="HEX6206784.1"
                     /translation="MGPSFRGFLAASGAAALLLAACTGGEAPTPATSPGTGSGPPVTP
                     DAAPADLTQLTREDLTRVLSPDSIPSIDEPRFVAPDEAEEWLSAQEPVAVLEIDGDAR
                     AYPHQIMTWHEIVNDVVGGTPVAVTYCPLCNSALAYPRKVGTRVLEFGTSGSLYNSAL
                     VMYDRQTESLWLHFEGLAIEGPLEGTHLELIPVQTLSFEQFRGEHPGGRVLSQDTGFN
                     RPYGQNPYDFYDTAERPFLFDGEVDARLPAIARVVGVEIDEEAVAYPYEALSSEEGAS
                     VVHDTVGGEDLVVFWKAGVRSALDNPEIELGRDVGSSGVFLRQLGGRELSFLVEGDRV
                     VDEQTGSVWSLAGRATGGPLEGQELTPVQHLDTFWFAWSSHNRETRIFEG"
     gene            complement(18689..19324)
                     /locus_tag="VF058_00345"
     CDS             complement(18689..19324)
                     /locus_tag="VF058_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206785.1"
                     /translation="MVTAALVVAAIALTGIGFFGLYRPWQLRWGATPYELDRTMAGDE
                     VVRGPTFNATRAVTIDAPPEAIWPWIVQIGFGRAGWYSYDLLDNLGRHSAERLVPDLQ
                     HMEVGDLVPLGPGKDSGMRVKDIENGRSMVWWDSKNHLTTWAWALDPMPTGGTRLVTR
                     VRARSSWRHPSTVMWMPLLEVADFPMTRKCLLGIKRRAETPVPVRIPQGLP"
     gene            19588..20157
                     /locus_tag="VF058_00350"
     CDS             19588..20157
                     /locus_tag="VF058_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206786.1"
                     /translation="MELSDRELWTVIHGLVLGTVFLLAFAGGLAGLWSLRPGFLTATG
                     IRERMRRLYLGMWVMAGVAWLTVITGTWIVYPWYREGLAGDDFAGCAGLLHPAAETCS
                     PRDFLLSEASGNTATWHEFGMEWKEHISWAAPLLATAVAFIVSYYGPRLIARPWLRAV
                     VITMFVAAFAAAAIGGAFGAFLNKTAPIV"
     gene            20167..20559
                     /locus_tag="VF058_00355"
     CDS             20167..20559
                     /locus_tag="VF058_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206787.1"
                     /translation="MADADAHYDVEHAPELAGLNLPEKPDGPAAAAMLAAAIGIFVLG
                     LATVLNEVSTGLHDVFERMEMGLGVGPLAGKTILATAAFIVSWLILGLAWRRKNPSLK
                     VWFTISLILGILGALGTFPPIFLAFAGD"
     gene            20556..20765
                     /locus_tag="VF058_00360"
     CDS             20556..20765
                     /locus_tag="VF058_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206788.1"
                     /translation="MSRDRADRVAAWATGVGIGLIAFMLTWLIGNRLIGLIWPPPVGP
                     TVALAGAVVTGGAVAVVWGRRLTRR"
     gene            complement(20901..22382)
                     /locus_tag="VF058_00365"
     CDS             complement(20901..22382)
                     /locus_tag="VF058_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010593686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6206789.1"
                     /translation="MTATLTHRYLAEYARRPINLILLVVVPVVFVALSAGVVADFAKL
                     LGADGEGAALESVTAGWAAAFLAGMAGFFHVGSSRNADRRLALAGMNTRRILAARLAS
                     GLVLALVAAGGALAALAFRTGIADVPRTLGATVMFAVVYLGIGAAVGAAVRDEVNGSL
                     VVLFVWLLDVFLGPAMGRTDAFLTRLFPTHFVTLVMTDAATGHAGSLGDLGAALAWTS
                     GAIGLAAAIFGITTRPTRGRARAAGPGGISRLAAGLRYGLREYRRNLTLWVLLITVPV
                     VLITLSFAVTPDAPTPVESGAATTVVSMVDLHGAIMVPITVGFLAGLAGLFVVLGSAQ
                     ADRRLVLAGFRPAEVLGARLGIVTLAAVLTTAVSLGVTAVDFRPAAWVPFVGASLLVA
                     LTYAMIGVLVGPMVGRLGGLFVMFLVPMIDVGIAQNAMFGATLPSWATYLPAHGAVRM
                     LLDGAFTQGFSETGGLLLALAWLAAITAVAALVFHRVAEPQRA"
     gene            complement(22379..23065)
                     /locus_tag="VF058_00370"
     CDS             complement(22379..23065)
                     /locus_tag="VF058_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6206790.1"
                     /translation="MTVTVEREVSNASAPGADRAVLAAEGLEKTFRRGVGPRRRVLHV
                     LRGASLHLCPGEIVGLVGENGSGKSTMMKILSGALPYDAGEIRRPGRIGYCPQEPILY
                     ERLTCDEHFELFGRAHGMSPEEMETSRARIYETLGFGSWAVARVEELSGGTRAKLNLG
                     LALLPDPQVLLLDEPYAGFDWETYQRFWELAEGRKAAGRAVLIISHFVTDEDLFDRIY
                     HMEDGRSVER"
     gene            complement(23062..23334)
                     /locus_tag="VF058_00375"
     CDS             complement(23062..23334)
                     /locus_tag="VF058_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX6206791.1"
                     /translation="MWHGWSGWADWIWMTVGMIAFWGLLIAVIVLAVRRGRTPERTGP
                     RPASAREILEERFARGEIDREEFEKSRAVLEAARPSKEHRSTKGAP"
     gene            23511..26480
                     /locus_tag="VF058_00380"
     CDS             23511..26480
                     /locus_tag="VF058_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX6206792.1"
                     /translation="MAEILVAIGGLGLIGGLAWYFFGPQEARRAEERGGVQEVEIAVK
                     GGYSPNLIRVRRGVPLRLVFDRQEGGECTSRVVFPDFSVSKSLPAFSRTTVQFTPDRA
                     GEFGFACGMNMVHGTLVVEPDGERPAGEEAGPAEGHSHEVTRAVGVGPTMQVGETMRV
                     EFAIRGEGVTCPTCVRTIESVLDPLPGVDRVEANFGAERVTVDYDPDQVSADDLEKAV
                     RSAGYRIEARPEPGSQETEDAEAEARRAEIRDLSRRVVLGAILTTPVLVAVMATEFFD
                     AAWVPELLLNRWTQLALITPVMLYTGWPIHVTGWLTLRHRTADMNTLITIGTTAAFLY
                     SLVVTIAPGALPEDVREVYYEAVGVIITLILLGRLFEARAKAGTGEAIRKLIGLQAKT
                     ARVIRGGTELEVPVEEVAPGDVVLVRPGEKVPVDGEIVDGRSTLDESMVTGESVPVDK
                     GPGDVVIGATVNQTGAFRFRATKVGRDTMLAQIVRLVEEAQASKAPIQRIADLVASYF
                     VPVVVFIAIGTFVLWFAFGPDPALIFGLTAAVAVLIIACPCALGLATPLSIMVGTGKG
                     AEQGILIRSATALETAHRLDAVILDKTGTITKGEPALTDVVPADGFTEEELLRLVASA
                     ERSSEHPLGEAIVSGAAERGLDLPEPSGFDSVTGKGIRATVDGREVLVGKAALLAEAG
                     IPASSLDELAARLAEEGKTAMFAAVDGAPAGVVAVADTVKEDSPRAIAALRRLGVETV
                     MMTGDNRRTAAAVAREVGIERVLAEVLPEDKAVEVRRLQDEGKLVAMVGDGINDAPAL
                     AQADVGIAIGTGTDVAIEAADVTLISGELKGAVTAITLSRATMRNIRQNLVFAFGYNT
                     AGIPIAAGALYAVAGVLLDPMIAAGAMALSSLSVVANANRLRRFRVRPLPEIGRAPEI
                     EPLVEVAEPEEAAPEPQTVTDPVCGMEIDPAGAAASEEHEGHMYFFCSTGCRDAFVAD
                     PERFTGSTVEAAHGHQG"
     gene            26525..26710
                     /locus_tag="VF058_00385"
     CDS             26525..26710
                     /locus_tag="VF058_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206793.1"
                     /translation="MSCGREGCQCGDAAELHGADHECGHPGDSDCCGGEHGAKAVTIE
                     RAESTAVAGDQSGEGWR"
     gene            26707..27747
                     /locus_tag="VF058_00390"
     CDS             26707..27747
                     /locus_tag="VF058_00390"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX6206794.1"
                     /translation="MTPVKVGVNGYGVIGKRVADAVTLQPDMELAGVADVVTDYRVRR
                     AVERGYPVYGSTADAARELGTAGFDPAGTLEDLLREVDVVVDATPKKIGAANKAAYER
                     AGVKAIFQGGEKHELTGVSFVAQANYEEALDGDLARVVSCNTTGITRVLSALRARDLV
                     ERARAVLIRRGTDPWESHAGGLINTLLPEAGVPSHQGPDAKTVIPDLDVVTMAAAGPF
                     NLAHVHFTWVEAPREVDRAEVLDAFREAPRIAFVRHADGVEAPNSVIEIVRDLGRPRA
                     DLWEVALWEDSLAVSGREIFLTYVVHNEAIVTPENVDAIRAVTGIETEAAKSIARTDE
                     TLGIRKELTWRP"
     gene            27735..>27947
                     /locus_tag="VF058_00395"
     CDS             27735..>27947
                     /locus_tag="VF058_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX6206795.1"
                     /translation="MEAVTGARRVDHPERRREKQVLDRLGRIQGQIAGIRRMYEEDRY
                     CIDVLDQVASARKALEATAQVILEDHV"
BASE COUNT         4193 a         9375 c        10143 g         4236 t
ORIGIN      
        1 gcccggcccc aggacgaacg tgtacgggat catcgggtcg ttctgcgggt ccgtgtactc
       61 ctggatgtcg aggtcttgct gcaccttgcg accggggtcc gacaggaacg cccactgcgc
      121 gcccagtccg tcgcgcagct cgttggtctc caggaggttg tccgtcgaga tcgtcacgat
      181 cctcgtgtat cccaccttga gctccggata gaggtccacg aggttccggt gctgtcgccg
      241 atccttgggg cagaagtgcc cccgcgaaag gacgaggatc atcgggtcgt ctccctgcag
      301 ctcggagagc gatctccggg tgcccgtgtg gtcggtcaac tcgtagtcgg ggaacgtccc
      361 cccgacggtg atgtctgatc gcatggtagg actcctttcg gatggctggg tttccttcac
      421 ggtcatggcg atgctcctct cggtctgtgg gtggtaccaa ccggccgcga agactggcca
      481 tccggaagtg tcagaagcca gacactcctc gggtcgatgt gagatcaggt cggcccgggg
      541 atgacgacgg ccggccgaga tgttgggatg cggagaaccc gaaggaggtg ctcgagtgag
      601 cgcgatcgac gattacgctc ttctgtcgga cggccatggg tccgcgctgg tcagcaagga
      661 aggatccatc gactgggcct gcctccctcg gttcgactca ccgtcggtct tcgggcggat
      721 cctcggcgac ggcgccggtc actggcggat cgctccttcc gcggacttcg agaccgagcg
      781 tgcctacctg gacgacacca tggtgctcag gaccaccttc cggtcgggcg gcggggcggt
      841 cgcgctgacc gacaccatgc cgttcgagcg agccgagggt gagcaccgga tcggccaccg
      901 cgccccccag gccgtcgtcc gttcgatcga ggggatcgag ggcgaggtcg agatggagct
      961 ggagctggcg ccgcgtccgg agtacgggct cacgaccccg acgctcgtgc cggtggaggg
     1021 tggggtcggc agcaggggag gcccgcaggc ctacgtcgtc tcctcgccgg tccccctgca
     1081 cctggaggag ggcgtcgcgc gcggcagctg taccgtgcgc gccggcgagc gcctcgcctt
     1141 cgccattcgg gtcggcagtc cctgggacgg gatggggcgt ccgtggggcc aggaggagat
     1201 cgtccgccgc ctggacgaga cgatcgacgc gtgggtggcg tggtccgcgg agcacgaggg
     1261 ctaccagggc ccgtaccgag agatcgtcca acacagcggc cgcgtgctgc aggcgctcac
     1321 gtacgtgccg accggagcgg tgatcgccgc ccccacgacc tcgctccccg agacgatcgg
     1381 cggcagccgg aactgggact accggtactg ctgggtccgt gacgccagcc tcacgctcaa
     1441 ggcgctctgg gtggcggcat gccccgacga ggcccagcag ttcttcgatt tcttcgtggt
     1501 cgcggcgggg gggcatctcc atgaggggcg cgagctccag gtcgtgtacg ggatcggcgg
     1561 ggagcgggac ctcgccgagc gggagctgac ccatctcgac gggttccgag ggagcaggcc
     1621 ggtccgcgtg ggcaaccggg cgtgggagca gttccagctc gacatctacg gggcgctgct
     1681 ggcggccgcg catctgctcg ccgagcggat ggggggcttc gacgatgcca cgggccactt
     1741 cctctgcgac ctggcgaaca tcgcggccgc tcgctgggag gagcccgacc aggggatctg
     1801 ggaggtccgc ggagaccccc agcacttcct gcattccaag ctgatgtgct gggtggcgct
     1861 ggaccgagcg gtgcggctgg cgccgatgat cggtggggag cgagacgtgg agcggtggtc
     1921 ggagattcgc gaccggatcc gagccgcgat cctggagcgt ggttggagcg atcgcgcagg
     1981 cgcgtacacg caggccttcg ggagcgatga gctcgacgcc tcggccctga tgatgccgat
     2041 cgtggggttc ctccctgccg acgactcgcg gatgcgggcc accatccacg ccgtctcatc
     2101 cgggctcacc gacgagcgag gtctggtgta ccgctacaag gcggccgatg ggcttcccgg
     2161 cgaggagggg gtcttcggga tctgcaccta ctggatggtc cagtgcctgg ccatgctcgg
     2221 cgaggtcgat cgggccaagg agctgttcga gaccatgacc tcgttcggca acgacgtggg
     2281 cttgttctcc gaggaggtcg atcccgggtc gggagaggca ctcggcaact tcccccaggc
     2341 cttcacgcac atcggcctcg tgaacgcggc ctgggtcatc gccgaggccg agcggggccg
     2401 gtcgtcccgg tccgacgatc aggccaaggg ccaggcctga tagagcagcc ccagaagggc
     2461 cccatagacc aggtgcccca tgaggctccc cacgggcgtc atcgcaccga agcgaacgcc
     2521 tgctggaccc gggtcctcct gctgtccgcg gcgaatggcc gggtggacgg agccgatcgt
     2581 cgtcatcatc aggagcgcca gggcccagag gatcgcgccc cagacgaggc cggcgatcca
     2641 gtcgggcgag agcccgacga gcgtgcccgc atacacgaac gccaccgcca ggacggctcc
     2701 gttcatatgg tggatgacgg ccccgatcac tcgtgagccg gtcgtatgcg gcgtggcgac
     2761 ggcgctcccg agaaggtcga ggagatccat gcgggtcatg cccatggccg tccccaccat
     2821 gccgaggatg gtgaaggcga cggtcgccac caccccccag acgatggcgc cctgccaact
     2881 gatcgctgcc gatccttcca ccgcgacccc gaccacgatg accgcgatgg gcaccgccat
     2941 gctcagcatc agcccgatcg gcccccacac gggatggtgg gtcgaacgcg tctccggcgt
     3001 tccgctgtgg acgaggttgg gacggactct ccgctcggta tcgagggccg ggtccatgtc
     3061 tcctcctttc cttgttctcc cgcttccgtc tcgcgggagt cttcatccct ccaaacgacc
     3121 gctccgcctc cagatcaccg aaccgttcgc ccgccgacac tgatgggtct ccggagtcag
     3181 ccgccccgat agcctgtccg tgtgacggaa ggaggatccg tgatcgccgt tcccggcgtc
     3241 tggtcggtcc ccctggtcgg agcgacctcg ctgctcgtgg tggtgggcgc cgttctcgtg
     3301 tcggggatcg cgcgcggacg gatcgcgatg gagctcggat gggggcggag caggatgtcg
     3361 ctccgggacc ggcgggtgga gatcgcggcg ccgcctgatg tcgtgctcca cctcctgcgt
     3421 caagcggccg cgggcgaggt gccgggcgtg acggagcgcg agcggaccga agtgatcgac
     3481 gccgacggtg acctgatcgt gaacgagtcg ctgaacgact cacggttcgg gatggtccgg
     3541 gcccgggagg cggtgcgctt ccatcccgac ggccgggtga cgtaccggca cctggcgggc
     3601 cctctcccgg ggaccgagga ggccttcgag gtgaagtccg ccgacggagg cagcgtgctc
     3661 acctaccacg gcgggatccc ggtggacttc tgggccctgg gccggctcgt ggctcgcttc
     3721 ctgatcctgc cggagtacga gcggctgctc gacgagcatc tcgcggccct gacgaagacc
     3781 gcagaggccc gcgcacgaag gcgccgaacc tgagtcaggc tcgggcgagg atcagcgcgt
     3841 aggagaggct gccgtcggcg gaagcggcgg cggcgatctc cagcagtcgt tggccgaggc
     3901 cgaggagctc cgtcgggatc cccatctcgg cggggttcac gacgccggcc gcgagcgcgc
     3961 cctggaagag ggccaggcgg cgtcgaacct gggccaggaa ctcgccgaga gcctctcggt
     4021 gatcctcgag ccgctcgacc gtgaagccgg cgtcctcgag cagccgcgcg tatccctgga
     4081 gcgggagggc gcctccggca caggcgatcc agcccatcag cgagtcgaga tcggcaggca
     4141 ggctgccctg gcgcgtgacg tccgtgagga cgaacgggga gccgggtcgg agaacgcggt
     4201 ggacctctcg tgccacggct tccttgcccg ggatcgctga gagcacgcac tcggcgagca
     4261 ccccgtcgag cgcctcgtcc gacaggggca gccgctccgc gtcggcagcc acgccggcca
     4321 ccccgggagc ctcagcggcc tccgtgaccg ctgaggtcga gaggtcgacc gccacgactc
     4381 ggcatcctct ctctgcggcc acccccgccc cggcgcccgg gccgctgccc acgtcgagca
     4441 gggtcgcctc ctcggggagg gcgaggaggt cgagggtgcg ctcggtgagg gcccgggcgc
     4501 cggggtggag atggtcgccc aacagcacgc ggacgccggg gtgggaccag aggtcggcgc
     4561 agcaggtctt caggaacgca gggtcgagta tgggcgggcc agcccgttgc ccttcgctcg
     4621 ttcggctgct cacgatccct ccggcgggac gggccgatgg tcaggggcga accgatacag
     4681 cgtgttgtac acacagaagg ggacgatccg gccgtccggc agcgcctcct ggatgcagca
     4741 gcgcttcgct cggttcacgt cgaaggtgaa tcggtccatg aaggcctcga tggtcaccgc
     4801 gaacatacgg tcgtgaaggt cgtccacggc atcgatctcg gccagaaggg gcccgcatgc
     4861 gcacgcgaac gcctcggcct gtccggggga gcccgccggg ttcgatttcg agaacagacg
     4921 gaggagcgcc tcccgcatct cctcggcgtc ctgccggaac gcctgggaaa gattcggcat
     4981 cgatgtgttg gcgacgtagt cgagataggt gtcgaccggc acgagccggg tcaccggcgt
     5041 gacgctcccg tcgctcacgt aggcgtaagc cgcggccgtg cagatcgggt cgcagcaggg
     5101 gatcgggatg aagtcctccc gcgtgaagat gccggagccc tcggcgacct tcttcaccac
     5161 ggcggcgacg tccatgcggt ccatcggatc gaagggcacg tagcgaccct cgccgaactc
     5221 gggctggagc gagaccgctc gcaccgccgg gtgggtggcg ccgaaccgaa cgagctcgcc
     5281 cacctgatcg tcgttcaccc cggcgagcac cgtcgccacg aggaccaccc gcgcaccggc
     5341 cacggcgagg ttctccagcg ctcgatgctt cacctcgagg agatccccac ggccgcgaag
     5401 cgttcggtag acatcctcgg tgaacccgtc gaactgcagg tacacgagca tctcgcgctc
     5461 cgcgatccct cgcgcgaatc cggggtcccg ggcgatgcgg atcccgttgg agttcacgag
     5521 gacgtatcgg atcggcttgg accgaaccag gtcggcgacc tcgaagaacc gtggatggat
     5581 cgtcggctcc ccgcccgaga gcatcacgac atccgcctcg ccgccctcgg ccgccaacac
     5641 ggcaccggtc atcgcctcca cgtcctccaa cgaggcgtgc ttggcgtgcg cctccccggc
     5701 gaagcacgcg gggcactcca ggttgcaggc ctcggtgatc tccaggacgg tcacgcaggc
     5761 gtgctgctcg tgctcggggc agaagccgca gtcgtagggg cagccgatct ccgactcggt
     5821 ggcggcgtgc gccggcttcc tgcccggacg taggaagcgc cgggagtcct cgtaccagcc
     5881 ggcgtcggag gagacgaggg cttcggcggt tccgtgctcc gggcaggtgc ggcggaggta
     5941 gacgcggttg tcacgctcca cgtgcgtggc ggaggacggt cggaggcaga tcgggcacag
     6001 cgacgtggtg ccgttcatcg accctcctct cgacctagag atccgaggcg tcgacgtagc
     6061 gccggcactc gtagcaccag gtgaaggcgt ccctgccgag cgggagggag cggatcacgg
     6121 gatgtctcgc cgtgagcgca tgctcgcggt tgtgacccgc ttgggactcg cagcacccca
     6181 cgaacccgca gtgggcgcac gccctgaggt tgtagccgct gccgcatgcc tcgcaggtct
     6241 cgctccgagg ctccacgacg ccctcgggga gatgcgtgca ggtcgccgcg gtcttgaaga
     6301 gtcccatcct cacctccgct gttgggggcg gccgctgtgg ggggcggcat ccacggctgt
     6361 gcataaccgg tccccgatgt ggacggggct cagaccgttc cccagggaac cgtttccttg
     6421 cattcggagc gttggagtgt tggcctagga acagtcgtct gtccaagggg agggtccgat
     6481 gacagggatg ttccgccagg ccgcatggct ggcgcgctcg ctgggccggg gcgacctggc
     6541 gccgttcacc gacgacgaca tccgtgagct ggcggagtcg atcggcctgc gcaccgtgga
     6601 ggccggcact ccgcttcttg agcaggggaa gccggtcgcc ttcatcgccg tgatccagcg
     6661 gggagacgtg ggcctctact accggtcggg gctccgccgg gtgatgctgc agaggctcca
     6721 tgagggcgac gtcctgggcg acgtgcccta cttctgccgc acgggatcgc cgttctccgc
     6781 ccggagcctc tccgaggtcg atctcctcca cctcgaggac gaggtgctca gacgcctcct
     6841 gtacacgcga cccgctgtca cgcagcggtt cctctacagc ctcgcaacca ggctcgagcg
     6901 gatgcagcgg cggctgctcg agatcaccca gcgtgatctc cggggccagg tggccacgct
     6961 cctcctgaac gagaccgagg agcagcccgg cgagatccat cttcctcagt ccacgctcgc
     7021 cgagctcctg ggcgcgacgc gcccgagcgt gaaccaggtg ctgaagggct tcgagaggga
     7081 gggattggtc cgcctctcgt accggcgact cgaggtcgtc gaccccgatg ggctccgccg
     7141 agccatctcc tgagggtggc tggatatcgg agcgggcctg cctcagctcg tcgcgatcgt
     7201 ccgccggatc cggtccacga gggagtcggg gacctcggag gacgcgccga gcttggtgcg
     7261 aagggtcgct cgaagtctcg agatgaacgc ctcgtgctcc aggcactcgg gacacgcccc
     7321 caagtgttcg gcgagggcgg cggcctccgg gttcgggagc tcgccgtcga ggtagacctc
     7381 gatatccctc agcacctctt cgcaggaggg cttcatcctc cggtcacccc gcgtcgctcc
     7441 atcgactccc acaacgcctt ccggagcgcg ttccttccgc ggtgcagccg cgacatcacg
     7501 gtcccccgcg ggatgtcgag gatgtcggcg atctccctgt agaggaaccc ctccacgtcg
     7561 gccagcagca cgaccattcg gaacctgtcc ggcagggagt cgagcgcgtc ctggacctcg
     7621 gcgtcgggca tccgctccag ggcctggacc tccgcggagg gcgcgaggtc ggactctcgg
     7681 atcttgtcgt acaggtacca gtcgggggcc tcatccgagg agaggatctc cggctcgcgc
     7741 cgtctcttcc ggtagatgtt gatgtaggtg ttcgtgagga tgcggtacag ccacgccttc
     7801 aggttcgtcc cctccctgaa tccggcgaag cctcggtagg cacgaagata ggtctcctgc
     7861 aggagatcct cggcgtccgc cgggttcctg gtcaggctca tcgcggccgg gtagagggtt
     7921 ttgagcagcg ggatggcctc ctcctcgaac cgcctcttca gcccatcctg cgacgcatcg
     7981 gtccgtacga gcgcgacccg ctgtcccgac atggcccatc tcctcgcgct gctgtaggcg
     8041 tgcgtcacga gccaaccaga gccacgttcc ggtgacaccg agagtgtgtc cccgccgaca
     8101 ctctcggcga agccaccgcc ggaggcggaa tcatggccgg cacagtcatg ttgcgcctca
     8161 aggagggact cgaccgtcga ggcccagggg agagactatg caggctcgcg aggatgatct
     8221 gcgtcccgac gtggtcgttc atacacacga gggaggcccg cctcgcggga cggacctcga
     8281 catcctgagc gtcaacgcca tccgggcctt ggccatggac gcggtggaag cggccggatc
     8341 cggacacccg ggcacgccga tggccctagc ccccgtcgcc tacgtcctgt ggacccgctt
     8401 cctccgacac gatccccatg atccggagtg gcccgatcgg gaccgcttcg tgctctccgc
     8461 gggacatgcc tcgatgctgc tctactcggt cctccacctc agcggttacg agctcccgct
     8521 ggatgagctg cgacacttcc gtcagtgggg ctcgcgcgca cccggacacc ccgagcgagg
     8581 gatcacgcct ggcgtcgagg tcacgaccgg cccgctgggt caagggttcg cgaacgcggt
     8641 ggggatggcc atcgcggagc ggatgctggc cgaccgcttc aaccgccccg gccacgacgt
     8701 cgtggatcac cgcacgtacg ggatctgctc ggacggggac ctcatggagg gggtctcggc
     8761 ggaggccgcc tcgctggccg gcgatctccg cctcggcaag ctcaccatgc tctacgacga
     8821 caaccacatc accatcgagg gctccaccga tctggcgttc tgcgagcagg tctccgagcg
     8881 cttccgcgcc tacggctggc acgtcctcca ggtgtccgac gcgaacgacc tcgaggaggt
     8941 cggtgctgcc ctggacacgg ctggcgccga gagcgagcgg ccatcgctca tcgtcgtccg
     9001 ctcggtgatc ggctacgggg ccccgacgaa gcaggggacg gccgcggccc acgggtcgcc
     9061 gctcgggccc gacgagctgc gcgccgcgaa agaacgactc gggtggcggt accccgagcc
     9121 cttcaccgtg ccggaggagg tgctcgctca cttccgcgac gttctcgacg aaggggtgag
     9181 ccctcacgcc gattggaggg agcgcttcgc ccgctaccag gaggagcacc cgggtctcgc
     9241 cgcggagttc tggcgcgtcc tctcgggtga gctcccctcg gggtgggacg acgccatgcc
     9301 gaccttcgag gttggggccg aggtcgcgac gcggtccgct tcgggctccg tcctgaacgc
     9361 catcgcgtcc gcggtcccgg aactggtcgg tgggtcggca gacctcgcgc cctcgaccga
     9421 cacctacctc gaggggttcg ccgacgtctc gtgcgcggac ttctcgggac ggaacttcca
     9481 cttcggcgtg cgggagcacg ccatgggtgc cgtcatgaat gggatggcgg cgcacggtgg
     9541 tttccgtccc tacgggggaa ccttcttcgt gttcagcgac tacatgcggc ccgcgatccg
     9601 gatggccgcc ctcatggggc ttcccgtcac cttcgtgctc acgcacgact ccatcggtct
     9661 gggcgaggac ggccccacgc accagccggt tgagcatctc gccggactcc gcgcgatccc
     9721 cgatctcgtc gtgatccgcc cggcggatgc gaacgagacg gcccaggcgt ggagggtgac
     9781 gatggagcga cgggaggggc ccgtggcgct cgtgctgacc cgccagaagc tcgccgtcct
     9841 cccgtcgctt cggacggacg ccgtcgcccg cggcgcccac gtggtggccg acggcgaggc
     9901 ccttgcgctc gtcgcctccg gatcggaggt ccacctcgcg ctcgctgcgc ggacactcct
     9961 ggcgtcatct tcgatctcgg cccgggtggt gtcgatgccc tcctgggagc tcttcgaggc
    10021 ccagtcggag gggtaccggg acgaggttct gccgccggga ctcccgacgc tcgcgatcga
    10081 ggcggcgagc ggcttcgggt ggaggaggtg ggcggacgac agcgtgtcgc tcgacaggtt
    10141 cggcgcctcg gcgcccgcgg aggtgctctt ccgcgagttc gggttcacgc ccgagcaggt
    10201 ggccgagcgc gctcgacggc tcctgcgcaa aggacataac ggatgatcct cttggcggtc
    10261 gatctcggag ggacccgcat gcgggcggcg ctcgtcgccg acgacgggcg gatcgtggag
    10321 cgccgtgacc ggcccactcc gcgggagcgg tcctgcgccg gcgcgctcct cgagctcgcc
    10381 ggaggagtcg tcgagggctc cggcgtgagg gaggccgtca tcggcgtgcc ggggcgcgtg
    10441 gactacggcg ccgggcggat ggagcacgct cccaacctgc ctgcccattg ggctcccgag
    10501 ctgaccgagg ggcacctctc ttcggcgctg ggtctgcggg tcgcgctcgc caacgacgcc
    10561 gatctggccg ccgtggggga gttccgcttc ggggccggcg ccgacgccag ggacgtggtc
    10621 tacctcacca tctcgacggg cgtcggggcg ggcgtgatcc tcggggggcg gctggtccgc
    10681 ggtcgccggt ccctcgccga ggccggccac accatcatcg accgttcgtc gctgaagcgt
    10741 ggcgagccgg ccacgttcga ggatcaagcg tcgggcaccg ccctggctcg cctggggagg
    10801 gcggtgggga tcgacgacgg cgccgcggcc gtgctcgagg cgacggtggc cggggacgcc
    10861 tccgcggccc gggtctggaa tcaggtcgtg gaggctgcgg ccgtgggcgc gacgaacctg
    10921 gctcacctgt tctcgccgga ggtgatcgtg gtgggcggcg gcgtcgccaa cgccggtgcg
    10981 ctcctcctcg atccgatccg tcggcatctg gacgagcacg ggccttcctc tctcccgagc
    11041 cccatcaggg tggtgggggc caggctgggc gacgacgccg ctctcgccgg agcgcccgcc
    11101 tggcgggaga tcgccctcgg ggaggtgggc gcccgtgcct agccgctccc cgcacgtcgt
    11161 cctcgtgcgc cacggggaaa ccgagtggac cgcccgtggg cgtcacacgg gacgcaccga
    11221 cgtgccgctg accgagaggg gacgcgagga ggccgggatg ctggccgaga ggctgcgatc
    11281 ttggagcttc ggccgggtgc tggccagccc gctgagccgg gccctcgaga cctgtcggct
    11341 ggccggatac ggcgaggggg ccgagatccg cgatgagctg atggagtggg actacggccg
    11401 ctacgaggga cgaaccacgg ccgagatcag gaagaaggtc gccggctgga gcatctggag
    11461 ccagggagcc ccggacgggg aggccgcaga ggacgtggga ggaaggatcg atccgatcgt
    11521 ccaggagctg cgggaggcgg aacacgacgt ggccgtgttc ggtcacgggc acctgctccg
    11581 ggccctcgcc gccaggtggc tcgagctccc tccggccgag gggcgccggt tcgcgctcga
    11641 cacgaccacc gtgagcgtgg tcgggcacga gcgggagacc ccggcgcttc tccgctggaa
    11701 cgaggactgc cacctcggcc cacgcgggga tgcgctgctg accccggcca caggagagga
    11761 cacgggagca tgaatccact gaggcagctg agggaggccg gacagagcgt gtggctggac
    11821 tacctccgcc gagggctgat cgccggcgga ggacttcagg agctgatcga cgaggacggg
    11881 gttgggggcg tgacctccaa tcccagcatc ttccggaagg cgatcggggg ttcaaccgac
    11941 tacgaggcgg cgatcgcggc tctggccggg aacgggacga acgcccgcga tgctttctac
    12001 cgtcttgcgc tggaggacat ccggtcggcc tgcgacgtct tcctgccgac ctgggaggag
    12061 accgaggggc tgcatggctt cgtctccttc gagctggaac cgggcctcgc ccgcgacacg
    12121 aaggggtcga tcgtcgccgc ggcggatctc gccgcgcgga tcgatcggcc caacgtgatg
    12181 atcaaggtcc cgggtaccga ggagggcgta cccgtcgtcg aggagctgac cgcggccggg
    12241 ctcaacgtga acatcaccct gctcttctcg gtcgaccgtc acgaggaggt ggcccgtgcg
    12301 tacgtcgccg gactgcggcg agggctcgag gccgggcagc agatcgaggg catcgcatcg
    12361 gtcgcctcct tcttcgtctc ccgtgtggac tcgaaggtgg atgcgaggct ccccgacgac
    12421 tcgcctctcc gcggccggat cgcgatcgcc aacgcgaaga tggcatatcg acggttccag
    12481 gagatcttct ccggggagga gtgggaggag ctggccgcgg caggcggccg ggtgcagcgc
    12541 ccgctgtggg cctcgacctc aacgaaggac cccgagcttc gtgacgtcct ctacgtggag
    12601 tcgttggtcg gtccccagac cgtcaacacg atgccccagg cgaccatgga tgcgttccgg
    12661 gaccacgggc gtgtggatcc cgcggcggta tcggatgggc tcgacgaggc ggaggacgcg
    12721 ctggcccggc tcgaggaggc tggcgtgtcg ctggagtccg tcacgtccga tctggtggag
    12781 gaggggatcg cggcgttcgc cgcggacttc gaagcgctcc tcgagacgat cgagggcacg
    12841 ctcgcgcggt cccgggcggg ggaatcccag cgggggtcgt cgctgggggc tctggacggt
    12901 ccggtccgcg cgcggctgga tgcactccag gagcggtcga tcgtccggcg gatctggcgg
    12961 aaggaccata cggtctggaa ggacgacccg accgagatcg tcgaccgact gggatggctg
    13021 accgtcgccg accggatgca cgagcggatc gaggagctgg aggcgttcgc caaggagtcc
    13081 gtcgccgacg ggttcgagac cgcggtcctc ctcgggatgg gcggctcctc gctggccccg
    13141 gagatgctca tgcggacgtt cggggcgacc ggagaccgcc tcgagctcgt ggtgctcgac
    13201 acgacgcatc cggtcgccat cgctcggatc gagacggagc tggacctcgc gcgcaccctg
    13261 ttcctggtgg cgagcaagtc gggagcgacc gtcgagacgc tgtcgcatct cgaccacttc
    13321 tgggagcgca cccgaggcgc ggccgagcgc ttcgtcgcca tcaccgatcc ggggacgccg
    13381 ctcgccgagc tcgcgaggga gcgggggttc cggaaggtgt ggatcaaccc ggaggacatc
    13441 ggcggacgat actcggccct ctcgctcttc gggctggtgc cgggcgcgct gatcggcgcg
    13501 ccgctccgcg agctcctcga cggggccgag gagatgggca tcgcctgcga cgcgtgcatc
    13561 gagtcgaagg acaaccccgc aggctggctc ggagcggtgc tcggggacgc ctcgctcagc
    13621 ggtcgggaca agttgacgct ggtgctcccg gaggagatcg cttccctcgg atcgtgggtc
    13681 gagcagctca tcgccgaatc gacggggaag gaggggaccg ggatcgtccc cgtggtcggt
    13741 gaggacctgg gggccccgga cgtctacggg cgggatcgac tcttcgtcgc ctacggcgac
    13801 cacgaggggc tcgaggcgat cgaagcagcc ggacaccccg tcgtgcggct cccgtacgac
    13861 ggcccgacct cgctcggccg agagctcttc cggtgggagt tcgccaccgc ggtggccggc
    13921 cacgtcctgg gcatcaaccc gttcgatcag ccgaacgtcg cggaggccaa gcaggcgacg
    13981 gccgagatcc tggagtcggg gattgaggag ccggggctcg atgacctgcc gccactgctg
    14041 gagcaggtgc ggccgggcga ctacctctcg ctccacgcct acctcgaccc caccgacgag
    14101 acggccgcgg tcctccatcg ggcccgtatg gccctgcgcg accgatacgc ggtcgccacc
    14161 accgtggggt tcggccctcg gttcctccac tcgaccgggc agctccacaa gggcgggccc
    14221 gccacgggag tgttcatcca ggtggtggac ccggaccggc ccagggacgt cgagatcccg
    14281 ggcaagccgt actccttcgg aaggctcatc gacgcccagg ccctgggcga cctccggtcc
    14341 ctgcggggcc gcgggcggcg ggtggccagg gtgacgctgg agcagctcct cgggggggtg
    14401 gcgtgatgca gatcgggatc atcgggctcg gccgcatggg cggcaacatg gcggagcggc
    14461 tccggcgcgg gggccatgag gtcgtggggt acgaccggag cggcgaacgc tcggacgtgg
    14521 gttccctgga ggagctcgcc gggaagctcg acgccgcgcg caccgtgtgg accatgatcc
    14581 ccgccggcga gcccaccgag caggccgtgc gcgagctggc cgggctcctc gagcccggcg
    14641 acgtggtcgt cgacggcggc aactcgaact tccgcgactc gatgcgtcgc gcccgggagc
    14701 tcgaggagcg aggcatcctg ttcgtcgacg tcggggtctc gggaggcgtc tggggtctcg
    14761 aggagggata cggtctgatg gtcgggggaa ccgatgaagc cgtcgcccgc ctcgtcccca
    14821 tcctcgatgt gctcgccccc gagggccgct acgtccacgt cggccccccc gggtcggggc
    14881 acttcgccaa gatggtccac aacgggatcg agtacgggat gatccaggcc tacgcggagg
    14941 ggtttgacat cctgcaggcc tcccgggagt tccccgacat cgacctgcac gccgtggcgg
    15001 aggcctggcg ccacgggacc gtgatccggt cgtggctcct cgacctcgcc gcggacgcgc
    15061 tggagaagga cccgcgcctg gaggatctcg aggcgtacgc ggaggactcc ggcgaggggc
    15121 gctggaccgt cctcgaggcg atcgataccg ccgtcccggt tccggtcatc gcggcggccc
    15181 tcttcgcccg gttctcctct cgagaagaga gcccgttctc catgcgcatg gtggccgcgc
    15241 tccgacgcga gttcgggggg cacgcggtga aggagcgatg agcgcggacg aggttcggcc
    15301 gcaggcggtc gtcatcttcg gagcctccgg tgacctgacg cggcggaaga tcctcccggc
    15361 cctgtacaac ctggcccggg agggcctgct gcccgagcgg tacgcggtcg tggggtacgc
    15421 gcggaccgag tgggacgacg aggcgttccg tgcccacgcc cgtcgggcca tccaggactt
    15481 ctcccgcacg ccggtggacg aggatgtgtg gaagcggttc tccgacgcga tctcgtacgt
    15541 ggccgggccc ttcgacgatc ccggctgctt cgcgccgctg agatggcgtc tcgaggatct
    15601 cgacgaggcg cgaggaacgg aagggcgacg cctctactac tgcgcgaccc cgccctccgc
    15661 gttccccgag atcgttcgcc ggctgggaga ggcggggatc ggcgaggatg cgaggatcgt
    15721 catcgagaag cccttcggcc acgacctgga gagcgcacgg agcctcaacc gcacggtcca
    15781 cgacgtcttc cacgaatccg cggtgttccg gatcgatcac tacctgggga aggagaccgt
    15841 gcagaacctc gtggtgctcc ggttcgcgaa ctccctgttc gaacgggtct ggaaccgcga
    15901 tgccgtcgac cacgtgcaga tcacggtcgc cgagcaggtc gggctggagg gtcggggcgc
    15961 ctactacgag gaggcgggcg cgctgcgcga catcgtccag aaccacgtgc tccagatgct
    16021 ggccctgctc gccatggagc ctccccggtc gctcgagccc gacgcgatcc gagacgagaa
    16081 ggtgaagctc ctgaacgccc tccgcccgct ggaccccggt ggcgtcgtcc ggggccagta
    16141 cgcgtcgggg acgagcgacg gggaggtcgt tccggggtac ctcgaggagg agggggtggc
    16201 cgccgactcg cagacggaga cctacgccgc gctgagggtc atcgtggaca actggcgatg
    16261 ggaaggtgtg cccttctacc tcaggaccgg caagcgcctt ccccgacgcg tgaccgaggt
    16321 gaccgtggtc cttcggcgag ccccggcgta cctgttcgcg gagctgggga tgagcccact
    16381 cgctccagac catctgacct tccggatcca gcccgacgag gggatctccc tcgctttcca
    16441 ggcgaaggag ccggggccgg ggatcgcccc caagacggta cggatggagt tcgactacga
    16501 ggggtcgttc gagcacaccc ccgccgaagc ctacgagcgt ttgctcctcg acgcgatggc
    16561 cggcgaccag accctcttca ctcgtgagga tggtgtggag cgcgcgtggc gggtggtgat
    16621 gccggcgctc gagcggccgg gtcccatcca tgcgtatccc gccggctcgt gggggccgaa
    16681 ggccgcggac gagctcatcg cgccgcggag ctggcacctg cactgagcct ccggatccgt
    16741 tccgcccgat gagaggagcg tgcgtcgtct ccgcgctgtc ccgagagcag gcggccatca
    16801 gtgtcgggtc gatgacaatc agggtcttcc gggggcaggt tcgaggttga gcgaaaggag
    16861 aggaaaccac cgaccatggg aggtacgccg aatggttctc cacgggatcc tggcagcgtt
    16921 catcgggacg ggcctcatga cgatatcgag cacgaccgag atgcagtggg cggggcgaga
    16981 ggcgagcgtc gcccccggcc tcgccaccgc caagctcctc aggcccttcg gtgttcgaga
    17041 ggtgaagggc cgggccctcg acctcctggc gacctggacc cattgggtgt acgggacact
    17101 gtgggggctc gccctgtggg ctttggtggg tcccgccggc cttcctctct gggccgccgg
    17161 gatcgccttc ttcttcctcg tgtggggcgc cgagcagatc cacctgccgg tgctcggcat
    17221 cgcgccctgg ccgtggaagt gggggctgaa agcgaacctc accgacatgt ggcatcacat
    17281 cgtgtacgcg gccggcgcca cgggcgcatg ggcgctcatc gacgtggcag ggtgatcgga
    17341 tgggcccgag cttccggggc ttcctcgcgg cgagtggagc ggcggcgctc ctgctcgcgg
    17401 cctgtaccgg gggagaggcg ccaacgccgg cgacctcgcc ggggacgggc tccggcccgc
    17461 cggtgacccc ggacgcggcg ccggccgacc tgacccagct caccagggag gatctgaccc
    17521 gggtcctgag ccccgactcg atcccctcga tcgacgagcc gaggttcgtc gctccggatg
    17581 aagcggagga gtggctctcg gcccaggagc cggtggcggt cctggagatc gacggggacg
    17641 cccgcgccta cccccaccag atcatgacct ggcacgagat cgtgaacgac gtcgtgggcg
    17701 gcacgcccgt ggcggtcacc tactgcccct tatgcaactc cgccctggcc tacccgcgga
    17761 aggtggggac gcgggtcctg gagttcggga cctccggaag cctctacaac agcgccctcg
    17821 tgatgtacga ccgccagacc gagtcgctct ggctccactt cgaggggctg gcgatcgagg
    17881 gaccgctcga agggacgcac ctcgagctca tcccggtcca gaccctctcc ttcgagcagt
    17941 tccggggcga gcatcccggg gggagggtgc tctcccaaga cacaggcttc aaccgtccct
    18001 acggccagaa cccctacgac ttctacgaca ccgccgagcg ccccttcctc ttcgacggcg
    18061 aggtggacgc gcggctaccg gcgatcgccc gggtggtggg ggtggagatc gatgaggagg
    18121 ccgtcgccta cccgtatgag gccctgtcct ccgaggaggg ggcgagcgtc gtccacgaca
    18181 ccgtgggggg agaggacctc gtggtcttct ggaaggcggg ggtccgctcg gccctcgaca
    18241 atcccgagat cgagctgggc cgcgacgtgg ggtcttccgg agtgttcttg cggcaactcg
    18301 ggggccgtga gctctcgttc ttggtagaag gggatcgggt cgtggacgag cagacgggct
    18361 cggtctggtc gctggcgggc cgggccaccg gcgggccgct cgagggccag gagctcacac
    18421 ccgtgcagca cctcgacacg ttctggttcg cctggtcctc ccacaaccgc gagacgcgga
    18481 tcttcgaggg ataggcttgg cacgatcccc gctgttgggt gttccatcgc gaacgggtgc
    18541 cggtccctca ctccagcaga tcggaaggtg cgagccctgc tgcggatccg tcccacctcg
    18601 aacccctctc catcgagaga cggagtgagc gccgcgccgt cgatcggtga cggggaagcc
    18661 tctcgtaagt cgcgcgattc ttccggggtc acgggagccc ctgggggatg cgaactggca
    18721 cgggcgtttc ggctcggcgc ttgatgccca gcaggcactt gcgcgtcatc gggaagtccg
    18781 cgacctccag tagcggcatc cacatcacgg ttgaggggtg acgccacgag gagcgagccc
    18841 gcacccgcgt tacgaggcgc gtgccacccg tgggcatggg gtccagggcc catgcccagg
    18901 tcgtcaggtg gttcttgctg tcccaccaga ccatggaccg gccattctcg atgtccttga
    18961 ccctcatgcc ggagtctttg cctgggccca gcggaacgag gtccccgacc tccatgtgct
    19021 ggagatccgg gacgaggcgc tcagcgctgt ggcggccgag gttgtccagc agatcgtagc
    19081 tgtaccaacc ggcgcgcccg aagccgatct ggacgatcca cggccagatg gcttcaggtg
    19141 gcgcgtcgat ggtgacggct cgagttgcgt tgaaggtcgg gccacgtacc acttcgtcgc
    19201 cggccatcgt gcggtcgagc tcataggggg tggctcccca cctgagctgc cagggccggt
    19261 acagaccgaa gaagccgatg cccgtcagtg cgatggcagc gacaacaaga gcggcggtca
    19321 ccatgtccac ttcgtccgcg tgcccatgac ctctccgtag ggcgcgggat agggagacgc
    19381 gcgctctggg tgcatgttcg cggcccgagc ggaggcctgg cagggccgaa cggccccctt
    19441 cctgcgggca agagcgggtc gctcctgatg gcaatgggcg aaccggaccg cccaacgttc
    19501 gatggctact caatcggcgc gacctcttgt aatcggtgcc atgatcatgc ttgattgagg
    19561 caaccgtagc gtgcgagagg aggcgcgatg gagctttcgg atcgcgagtt gtggacggtc
    19621 atccacggcc tggtcctcgg aaccgtgttc ctgctggcgt tcgccggtgg actcgccggg
    19681 ctctggagcc tccgacccgg attcctgacg gcgaccggca tccgcgaacg catgcgccgt
    19741 ctctaccttg ggatgtgggt gatggcaggt gtggcatggc tcacggtgat cacaggcacg
    19801 tggatcgtgt acccgtggta ccgagagggg ctcgccggcg acgacttcgc cggatgcgcg
    19861 gggctgctgc accccgcggc ggagacgtgt tccccgcgag acttccttct gtccgaggca
    19921 tcgggcaaca cggctacctg gcacgagttc ggcatggagt ggaaggagca catcagctgg
    19981 gccgcgccac tcctggccac cgccgtcgcg ttcatcgtct cgtactacgg gccgcgtctg
    20041 atcgccaggc cgtggctgcg cgccgtcgtg atcacgatgt tcgtggccgc gttcgcggcc
    20101 gcggcgatcg gcggagcgtt cggtgccttc ctcaacaaga ccgccccgat cgtttaggag
    20161 gcttcgatgg ctgatgcaga cgctcactac gatgtcgagc acgctcccga gctggccggg
    20221 ctgaacctcc ctgagaagcc agacgggccg gcggccgcgg cgatgcttgc cgcagcgatc
    20281 gggatcttcg tcctcggtct cgccaccgtc ctcaacgagg tctcgacggg gctccacgac
    20341 gtcttcgaga ggatggagat gggcctcgga gtcgggccgc tcgcagggaa gaccatcctc
    20401 gccacggcgg cgttcatcgt gagctggctc atcctcgggt tggcctggcg gaggaagaac
    20461 ccgagcctca aggtgtggtt cacgatctcc ctgatcctcg gcatcctcgg cgcgctggga
    20521 acgttcccgc cgatcttcct ggcgttcgcg ggagattgag ccgcgaccgg gccgaccgcg
    20581 tcgcggcgtg ggccacgggg gtcggcatcg gtttgatcgc gttcatgctg acgtggctga
    20641 tcgggaaccg actgatcggg ctgatctggc ccccgcctgt gggaccgacg gtggccttgg
    20701 cgggggcggt tgtgaccggc ggagccgttg cggtggtctg gggccgcagg ctcactcgac
    20761 gctgagcatc catgggctcg tcgccgcagc agcgcacgag acgatcgaag cgctcccccc
    20821 tgctcaggtg gtttcgaccg gcatctggcc cgcgcatgcc gagcagaacg gggaaggtca
    20881 acacccagcc gttttcccgc tcacgcgcgc tgcggctccg ccacccggtg gaacaccagc
    20941 gccgcgaccg cggtgatcgc tgcgagccac gccagggcca ggagcagacc gccggtctcc
    21001 gagaaccctt gcgtgaaggc accgtcgagg agcatccgaa cggcaccgtg ggcggggagg
    21061 tacgtggccc aggacggaag tgtcgctccg aacatcgcgt tctgggcgat gcccacgtcg
    21121 atcatcggga ccaggaacat cacgaacagg cccccgagcc tgcccaccat cgggccaacg
    21181 agcaccccga tcatggcgta ggtgagcgcg accaggaggc tggctccgac gaacgggacc
    21241 caggccgccg gccggaagtc caccgctgtc acgccgaggg agaccgcggt cgtgagcacc
    21301 gcggccagcg tcacgatccc caggcgcgcg cccagcacct cggcaggacg aaagcccgcc
    21361 agtaccaggc gccggtccgc ctgggccgac ccaagcacca cgaacagccc ggcgaggccc
    21421 gcaaggaacc ccacggtgat cgggaccatg atggccccgt ggaggtcgac catcgacacg
    21481 accgtcgtcg cggcccccga ctccaccggc gtgggagcgt cgggggtgac ggcgaagctg
    21541 agggtgatga ggacgaccgg gacggtgatc aggagcaccc acagagtgag gttccgccgg
    21601 tactcgcgga ggccgtagcg gagtccggcc gcgagtctcg agatgcctcc cggtccggcc
    21661 gcccgagccc ggccgcgcgt cggccgggtc gtgatcccga agatcgcggc ggccagcccg
    21721 atagccccgg acgtccatgc gagggcggcc ccgaggtcgc ccagcgaacc ggcatggccg
    21781 gtggccgcat cggtcatgac cagcgtgacg aagtgcgtgg ggaacaggcg ggtgaggaag
    21841 gcgtcggtcc ttcccatcgc cgggccgagg aagacgtcca gcagccagac gaacaggacg
    21901 acgagagagc cgttcacctc gtcacgaacg gcggccccca ccgccgcccc gatccccagg
    21961 tacacgacgg cgaacatgac cgtggccccg agggtgcggg ggacgtcggc gatgccggtc
    22021 cggaaggcca gggcggcgag cgctccgccc gcggccacga gcgcgagcac cagccccgag
    22081 gcgagccgcg ctgcgaggat gcgccgggtg ttcatccccg ccagcgccag gcggcggtcg
    22141 gcgttgcggg agctcccgac gtggaagaac ccggccatcc cggcgaggaa ggccgccgcc
    22201 caccccgcgg tgacggactc gagggcggct ccctctccgt ctgcgccgag cagcttggcg
    22261 aagtcggcca ccaccccggc ggagagggcc acgaagacca ccggaaccac gaccaggagg
    22321 atcaggttga tcgggcggcg cgcgtactcg gcgagatagc ggtgggtgag cgtggcggtc
    22381 atcgctccac gctcctcccg tcctccatgt ggtagatgcg gtcgaagagg tcctcgtcgg
    22441 tgacgaagtg actgatgatc aggaccgccc ggccggcggc cttccggccc tcggccagct
    22501 cccagaaccg ctggtaggtc tcccagtcga accctgcgta gggctcgtcc agcagcagca
    22561 cctgggggtc gggtaggagc gccaggccca ggttcagctt cgcgcgcgtg ccgcccgaca
    22621 gctcctccac gcgggcgacc gcccaggagc cgaagcccag cgtctcgtag atcctcgccc
    22681 tggaggtctc catctcctcg ggggacatcc cgtgggcgcg gccgaagagc tcgaaatgct
    22741 cgtcgcaggt gagccgctcg tagaggatcg gctcctgagg acagtacccg atcctccccg
    22801 ggcgacggat ctcgcctgcg tcgtagggga gggcgccaga gaggatcttc atcatcgtgc
    22861 tcttgcccga gccgttctcg ccgaccagtc cgacgatctc gcctgggcag aggtgcaggc
    22921 tggcgccacg gagcacgtgg agaaccctcc gcctcggtcc gaccccgcgc cggaacgtct
    22981 tctccaggcc ctcggccgcg aggacggctc gatccgcccc cggggcggac gcgttggaga
    23041 cctcccgctc gacggtgacg gtcatggcgc tcccttcgtg ctccggtgct ccttcgaggg
    23101 tctggccgcc tcgaggacgg cccgactctt ctcgaactcc tcgcggtcga tctccccgcg
    23161 ggcgaagcgc tcctcgagga tctcccgcgc ggaggccggg cgagggcccg ttcgttccgg
    23221 cgtcctcccc cggcgcaccg ccaagacgat caccgcgatc aggagacccc agaacgcgat
    23281 catgccgacg gtcatccaga tccagtcagc ccatccgctc cacccgtgcc acatggctgc
    23341 accctccttc agccggtcgg gccctagtct tctacccctg gggggtatag gcatgggtcg
    23401 aaggtcccag atatggaggg accttctcct cgcgaacgcg aggggtacag catctagggt
    23461 gctggggtaa ggcacggaac agaaccggac tgagagggga cggggtggcg atggcggaga
    23521 tcctggtggc gatcggcggg ctgggtctga tcgggggcct cgcctggtac ttcttcggtc
    23581 cccaggaggc ccggcgggcc gaagagcgcg gaggggtcca ggaggtggag atcgccgtca
    23641 agggcggcta ctcgccgaac ctcatcaggg tccgacgcgg cgtgccgctg cgcctggtct
    23701 tcgaccgcca ggagggcggg gagtgcacct caagggtagt cttccccgac ttctcggtga
    23761 gcaagtcgct cccggcgttc tcacggacca cggtgcagtt cacccccgac cgggccgggg
    23821 agttcgggtt cgcctgcggg atgaacatgg tccacggcac cctcgtggtg gagccggacg
    23881 gggagcgccc ggcgggcgag gaggcgggtc ccgcggaggg gcactcccac gaggtcaccc
    23941 gagccgtggg ggtgggtccg acgatgcagg tcggtgagac catgcgggtg gagttcgcga
    24001 tccggggcga gggggtgacc tgtccgacat gcgtccgcac gatcgagagc gtcctcgacc
    24061 cgctccccgg cgtcgaccgg gtggaggcga acttcggcgc cgagcgggtc acggtcgact
    24121 acgacccgga ccaggtctcc gcggacgatc tggagaaggc cgtacgctcc gccgggtacc
    24181 ggatcgaggc gcgtcccgaa ccgggctctc aggagaccga ggacgccgag gctgaggccc
    24241 gccgggcgga gatccgggac ctctcgcgcc gggtggtgct gggggccatc ctcaccaccc
    24301 ccgtgctagt ggccgtgatg gccacggagt tcttcgacgc cgcctgggta cccgagctct
    24361 tactcaaccg ctggacgcag ctcgcgctga tcaccccagt gatgctctac acgggctggc
    24421 cgatccacgt gaccgggtgg ctcacgctcc ggcatcgcac cgccgacatg aacacgctca
    24481 tcacgatcgg gaccacggcc gccttcctct actccctcgt cgtcacgatc gcgcccggtg
    24541 cactgcccga ggatgtccgg gaggtctact acgaggccgt cggcgtcatc atcaccctga
    24601 tcctgctggg gcgcctcttc gaggccagag ccaaggccgg gacgggggag gcgatccgca
    24661 agctgatcgg cctccaggcc aagacggccc gcgttatccg cggcggaacc gagctggagg
    24721 tcccggtcga ggaggtcgca cccggggacg tggttctggt ccgccccggg gagaaggttc
    24781 ccgtggatgg ggagatcgtg gacgggcggt ccacgctcga cgagtccatg gtcacggggg
    24841 agtcggtccc ggtcgacaag ggcccgggcg acgtcgtgat cggcgccacc gtcaaccaga
    24901 cgggcgcctt ccgcttccgg gccacgaagg tcgggcgcga cacgatgctc gcccagatcg
    24961 tgcgactggt ggaggaggcg caggcctcca aggcgccgat ccagcggatc gcggacctgg
    25021 tcgcctccta cttcgtcccg gtggtggtct tcatcgcgat cgggacgttc gtgctctggt
    25081 tcgctttcgg ccccgacccg gcgctcatct tcgggctcac cgcggccgtg gccgtcctca
    25141 tcatcgcctg cccgtgcgcg ctggggctgg ccaccccgct ctcgatcatg gtggggaccg
    25201 gcaagggagc cgagcagggg atcctgatcc gctcggcgac cgccctcgag accgcgcaca
    25261 ggctcgacgc cgtgatcctc gacaagacgg gaacgatcac gaagggggag ccggcgctca
    25321 ccgacgtcgt gcccgcggac ggcttcacag aggaggagct gctgcggctg gtggcctcgg
    25381 cggagcgctc atcggagcat cccctcgggg aggcgatcgt cagcggcgct gccgagcgcg
    25441 gcctcgacct gcccgagccc tcgggattcg actcggtgac cgggaagggg atccgggcca
    25501 cggtcgacgg ccgggaggtt ctggtcggga aggccgcgct cctcgccgag gccgggatcc
    25561 ctgcctcctc gctcgatgag ctggccgccc gactggcgga ggaggggaag accgcgatgt
    25621 tcgccgccgt cgacggcgcc cccgcgggcg tggtggcggt ggccgacacg gtcaaggagg
    25681 attccccccg ggcgatcgcg gccctccggc gtctgggcgt cgagaccgtg atgatgacgg
    25741 gagacaaccg ccgaacggcg gccgcggtgg cgcgtgaggt ggggatcgag cgggtgctcg
    25801 cggaggtgct ccccgaggac aaggcggtgg aggtccgccg cctccaggac gaggggaagc
    25861 tcgtggcgat ggtgggggac gggatcaacg acgctcccgc cctcgcccag gccgacgtgg
    25921 ggatcgcgat cgggaccggg accgacgtgg cgatcgaggc ggccgacgtc acgctgatct
    25981 cgggggagct caagggcgcg gtcacggcga tcaccctcag ccgggccacg atgcgcaaca
    26041 tccgccagaa cctcgtgttc gccttcggct acaacaccgc gggcatcccg atcgcggccg
    26101 gcgccctcta cgcggtggcc ggcgtgctgc tcgacccgat gatcgcggcc ggggcgatgg
    26161 cgctctcctc gctgtcagtg gtcgcgaacg cgaaccggct gcggcgcttc cgcgtccgcc
    26221 cgctgccgga gatcggacgg gcaccggaga tcgagccact cgtcgaggtg gccgagcccg
    26281 aagaggccgc gccggagccg cagacggtga ccgaccccgt gtgcgggatg gagatcgacc
    26341 cggccggggc cgcggcctcc gaagagcacg aggggcacat gtacttcttc tgctcgacgg
    26401 gatgccgcga cgcgttcgtc gcggaccccg agcggttcac gggatcgacg gtggaggcag
    26461 cccatgggca tcagggttga gccgggcgtg ggtcggatga acgcgggttg agaaggaggc
    26521 tgcgatgagc tgtgggcgcg agggatgtca gtgcggggac gccgcggagc tgcacggcgc
    26581 cgaccacgag tgcgggcacc cgggggactc cgattgctgc ggtggtgagc acggcgcgaa
    26641 ggcggtgacg atcgagaggg cggagtcgac tgcggtcgca ggagaccagt ccggggaggg
    26701 atggagatga ctccggtgaa ggtgggagtc aacggctacg gcgtgatcgg caagcgggtc
    26761 gcggacgcgg tgacgctcca gcccgacatg gagctggccg gggtggccga cgtggtcacc
    26821 gactaccgcg tgcgccgtgc ggtcgagcgc ggctacccgg tctatggatc gaccgcggac
    26881 gccgcccgcg agctgggaac cgccggattc gatcccgccg gcaccctgga ggacttgctt
    26941 cgcgaggtgg acgtggtggt cgatgcgacc ccgaagaaga tcggggcggc gaacaaggcc
    27001 gcctacgagc gggccggcgt gaaggcgatc ttccagggcg gggagaagca cgagctcacg
    27061 ggcgtctcct tcgtggcgca ggccaattac gaggaggccc tcgatgggga ccttgcgcgt
    27121 gtcgtctcct gcaacacgac ggggatcacc cgggtcctct cggcactccg cgcccgcgat
    27181 ctggtcgagc gggcccgcgc ggtgctgatc cgccggggca cggacccctg ggagtcgcac
    27241 gcgggagggc tgatcaacac cctgctgccg gaagcgggcg tgccgtccca ccagggcccc
    27301 gacgccaaga ccgtcatccc cgacctcgac gtggtgacca tggccgccgc gggcccgttc
    27361 aacctggccc acgtccactt cacgtgggtc gaggcacccc gggaggtgga tcgggcggag
    27421 gtcctggacg ctttccgcga ggccccccgc atcgccttcg tccgccatgc ggatggcgtc
    27481 gaggccccga actcggtgat cgagatcgtg cgggacctgg gccggccccg ggccgacctg
    27541 tgggaggtcg cgctgtggga ggactcgctg gccgtctccg gccgggagat cttcctcacc
    27601 tacgtggtgc acaacgaggc gatcgtcacc cccgagaacg tcgacgcgat ccgggcggtg
    27661 accgggatcg agacggaggc cgcgaagtcc atcgcccgca ccgacgagac cctggggatc
    27721 cgaaaggagc tgacatggag gccgtgactg gagctcgccg cgtggaccac cccgagcgac
    27781 ggagggagaa acaggtcctc gaccgcctcg gccgcatcca gggccagatc gcagggatcc
    27841 gccggatgta cgaggaagac cgctactgca tcgacgtcct tgaccaggtg gcctcggcgc
    27901 gaaaggcgct cgaggcgacc gcgcaggtga tcctggagga tcacgtg
//
LOCUS       DASUTW010000010         4534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773 k141_3330,
            whole genome shotgun sequence.
ACCESSION   DASUTW010000010 DASUTW010000000
VERSION     DASUTW010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3330"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1008
                     /locus_tag="VF058_00400"
     CDS             166..1008
                     /locus_tag="VF058_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX6206796.1"
                     /translation="MEADTREDLIARLEEAARDLRITDIEMLAEAGSGHPGGTLSAAE
                     MVAALFFHKLRLRPEEPEWPDRDRFVLSKGHCIPIVYAAMAKLGFFDEKELMTLRKLG
                     TRLQGHPDRRLLPGIEASTGSLGQGLSVAAGMALAAKLDGADWRVYCMMGDGEIQAGQ
                     MWEAAMFAPRHGIDTLTAILDHNQVQQTGKVAETMPIEPIVAKWTAFNWHVREIDGHD
                     MVQVLDALDEAEMVTGKPTIIVSHTVKGKGVSWMELETAWHGKAPSPEEAERAIAELR
                     GEGS"
     gene            1008..1937
                     /locus_tag="VF058_00405"
     CDS             1008..1937
                     /locus_tag="VF058_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6206797.1"
                     /translation="MGAATRDAFGEAIVRVGEDPRIVVLTGDLKDSTRTDSFEEAYPD
                     RFIECGIAERNMIGVASGLALSGKIPWAASFSTFVAGRFETVRVSVAFQEANVRIVGT
                     HVGVGVGEDGYSQMAFEDVALMRTLPNMTIIQPADAAETHAAVEYLVEHEGPAFLRLT
                     RQGVDDVHGEGYRFEPGRIERLRDGDDVALIASGATVAEALRAADLLSEDGISARVLN
                     VHTIKPLDEEAVLQAASECDALLTVEDGSAVGGLGGAVAETLATRDGRAPLRIIGHRT
                     FGESGSPDELYEKYGLSGPRIAESTRDLLKTLG"
     gene            2028..2714
                     /gene="ftsE"
                     /locus_tag="VF058_00410"
     CDS             2028..2714
                     /gene="ftsE"
                     /locus_tag="VF058_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX6206798.1"
                     /translation="MIKTESVTKTYKDTVTALEDVSVEVEKGEFVFIVGPSGSGKSTF
                     IRLLLKEEEPTRGAIYVAGKNINTLSSWKVPYLRRNIGTIFQDFKLLPDKTVFENVAF
                     ALEVIGKPKHVIQKRVPEILTLVGLGGKLENYPDELSGGEQQRVSIARAFVNRPLILL
                     ADEPTGNLDPSTSVEIMKLLDRINRTGTTVIMATHDNAIVDAMRRRVIELEDGSVVRD
                     QARGVYGYGT"
     gene            2704..3588
                     /gene="ftsX"
                     /locus_tag="VF058_00415"
     CDS             2704..3588
                     /gene="ftsX"
                     /locus_tag="VF058_00415"
                     /inference="COORDINATES: protein motif:HMM:NF038346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX6206799.1"
                     /translation="MGRRFGYFFRETAAALRRNGLVTFAAISTVFISLFLLGGSLLVQ
                     KQVDLMAGDWAQKVEVSVFLRDEASQGEIETLSRKITGMPEVQQVFYESKEQAYDRFV
                     DLFKDSPALVENVSAESLPASFRVKLHDPEKFAVISARLAGEPAIDTIQDERRVLNKL
                     LAVTGILRTGAQAVAAIMLIAAGGLIGNTVRMAVFARRKEIAIMKLVGATNWFIRIPF
                     LIEGMVQGLLGAGFAILGLFVMKVLFIDPLRSQSLFGFIPWFGTSHILFTVPILLVAG
                     VLIATVASLFAMRRFLEV"
     gene            3654..>4534
                     /locus_tag="VF058_00420"
     CDS             3654..>4534
                     /locus_tag="VF058_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206800.1"
                     /translation="MKRLFLVVCALAVALPLPAAEAGPEQQLDRIQDELERQRELLQR
                     TNEEREGLLDEISDADRRRGDLTAKIEALQGDLALAEERLQEAQASLDLARASLRRWT
                     NRLEGARDRLTAQRDTLSSRAAAAYMMGAGGLVEVILGAEDLRSLTDRLEYVRSVMDS
                     DSYLLSSIQATRVQVAERRDHVADQESVLSEHRDAIRQEVERIAQLKAEQEALRAEVD
                     AAIAERERLLEGVEENRAQHVKAVKELEAESARIAGLIQGGGSSGSGNPNAQLFWPAP
                     GSITSGFGWRVHPIFGTR"
BASE COUNT          705 a         1541 c         1605 g          683 t
ORIGIN      
        1 gaccaggcgg gggaggcgcc ggtccgagcg cgaccagggg accctgagtc gctcggtccc
       61 ctcgtcggtc ccgtggtcgg tcgcggcgtc ggttcggtcg gtcatcgagg ctcgatgcag
      121 cgttgataga ctaccgatga atcctccccc gacgcaggag ccgccatgga agccgacacc
      181 cgcgaggacc tgatcgcccg cctggaggag gccgccaggg acctccggat caccgacatc
      241 gagatgctgg ccgaggccgg ctccggccac ccgggcggga ccctctccgc cgccgagatg
      301 gtggccgccc tgttcttcca caagctccgc ctccgccccg aggagcccga gtggcccgac
      361 cgcgaccgct tcgtgctttc gaaggggcac tgcatcccga tcgtctacgc ggccatggcc
      421 aagctggggt tcttcgacga gaaggagctg atgacgctcc gcaagctcgg cacgcgcctc
      481 cagggccacc ccgaccgccg gctcctcccg gggatcgagg cctccacggg gtcgctgggc
      541 caggggctct ccgtcgccgc cgggatggcg ctggccgcga agctcgacgg cgccgactgg
      601 cgcgtgtact gcatgatggg cgacggcgag atccaggccg gccagatgtg ggaggcggcg
      661 atgttcgccc cgcgtcacgg gatcgacacc ctcaccgcga tcctcgacca caaccaggtc
      721 cagcagacgg ggaaggtggc cgagacgatg ccgatcgagc cgatcgtggc caagtggacc
      781 gcgttcaact ggcacgtccg cgagatcgac gggcacgaca tggtccaggt cctcgacgcg
      841 ctggacgagg ccgagatggt gacggggaag ccgacgatca tcgtctcgca caccgtcaag
      901 ggcaagggcg tctcctggat ggagctcgag accgcctggc acgggaaggc cccgagcccc
      961 gaggaggccg agcgggcgat cgccgagctg aggggggagg gatcctgatg ggggcggcca
     1021 ccagggacgc gttcggcgag gcgatcgtcc gcgtgggcga ggaccctcgc atcgtcgtgc
     1081 tgaccgggga cctcaaggac tccacccgca ccgattcctt cgaagaggcc taccccgatc
     1141 ggttcatcga gtgcggcatc gccgagcgga acatgatcgg ggtcgcctct gggctggcgc
     1201 tctcggggaa gatcccgtgg gcggcgtcgt tctcgacgtt cgtggccggc cggttcgaga
     1261 ccgtgcgggt gtccgtggct ttccaggagg cgaacgtgcg gatcgtgggc acccacgtcg
     1321 gggtcggcgt gggtgaggac ggctactcgc agatggcctt cgaggacgtc gcgctgatgc
     1381 ggaccctccc gaacatgacg atcatccagc cggccgacgc ggcggagacc cacgccgccg
     1441 tcgagtacct cgtcgagcac gagggcccgg ccttcctccg gctgacccga cagggcgtgg
     1501 acgacgtcca cggggagggc taccggttcg agcccgggcg gatcgagcgg ctgcgggacg
     1561 gcgacgacgt cgcgctgatc gccagcggcg cgacggtggc cgaggctctc cgggccgccg
     1621 acctcctgtc cgaggacggg atctcggccc gcgtcctcaa cgtgcacacg atcaagcccc
     1681 tggacgaaga ggccgtgctc caggccgcct ccgagtgcga cgcactcctc accgtcgagg
     1741 acggcagcgc cgtgggcggg cttggggggg cggtggccga gacgctggcc acccgcgacg
     1801 gacgagcgcc gctccgcatc atcggtcacc ggacgttcgg cgagtcgggc tcgcccgatg
     1861 agctctacga gaagtacggg ctgtccgggc cccggatcgc cgagagcacg cgggacctcc
     1921 tcaagacgct gggctgaccc tccggtaccc tgttcgcggc ccggggaggg tgtttgggtt
     1981 ccctcgtggg gtctgatacg ttgtcgagcg gcccggaggg gccatccatg atcaagaccg
     2041 agagcgtcac caagacgtac aaggacactg tcaccgccct cgaggacgtc tccgtggagg
     2101 tcgagaaggg cgagttcgtc ttcatcgtcg gtccctcggg gtcggggaag tccaccttca
     2161 tccgcctcct gctgaaggag gaggagccca cgcgcggagc gatctacgtc gcggggaaga
     2221 acatcaacac cctctcctcg tggaaggtgc cgtaccttcg gcgcaacatc ggcacgatct
     2281 tccaggactt caagctgctg cccgacaaga ccgtcttcga gaacgtagcc ttcgccctgg
     2341 aggtgatcgg gaagcccaag cacgtgatcc agaagcgggt ccccgagatc ctcacgctcg
     2401 tgggcctcgg ggggaagctc gagaactacc ccgacgagct ctcgggcggc gagcagcagc
     2461 gcgtctcgat cgcccgggcg ttcgtcaacc gccccctgat cctgctggcg gacgagccca
     2521 cggggaacct cgacccctcc acctcggtcg agatcatgaa gctgctggac cgcatcaacc
     2581 gcaccggcac cacggtgatc atggccaccc acgacaacgc gatcgtggac gcgatgcgcc
     2641 gccgcgtgat cgagctcgag gacggcagcg tcgtccgtga ccaggcgcgg ggtgtctacg
     2701 gctatgggac gtagattcgg ctacttcttc cgcgagacgg ccgccgcgct ccgccgcaac
     2761 ggcctcgtca ccttcgccgc gatctccacc gtcttcatct cgctcttcct gctgggcggg
     2821 tcgctgctgg tccaaaagca ggtcgacctg atggccggcg actgggccca gaaggtggag
     2881 gtctcggtct tccttcggga cgaggcttcc cagggcgaga tcgagaccct cagccggaag
     2941 atcaccggca tgcccgaggt gcagcaggtc ttctacgagt cgaaggagca ggcctacgac
     3001 cggttcgtgg acctcttcaa ggactcgccg gccctcgtgg agaacgtcag cgccgagtcg
     3061 ctcccggcct cgttccgggt gaagctgcac gacccggaga agttcgccgt catctcggcc
     3121 cggctggcgg gggagcccgc catcgacacg atccaggacg agcgccgcgt gctgaacaag
     3181 ctgctggcgg tgacggggat cctgcggacc ggcgcgcagg ccgtggcggc gatcatgctg
     3241 atcgcggccg ggggattgat cggcaacacc gtgcggatgg ccgtcttcgc tcgccgcaag
     3301 gagatcgcga tcatgaagct cgtcggggcg acgaactggt tcatccggat ccctttcctg
     3361 atcgagggga tggtgcaggg cctcctgggc gcgggcttcg ccatcctggg gctcttcgtg
     3421 atgaaggtgc tcttcatcga tccgctgcgg agccagtcgc tcttcgggtt catcccgtgg
     3481 ttcgggacga gccacatcct cttcaccgtc cccatcctgc tggtcgcggg cgtgctgatc
     3541 gccaccgtgg cgagcctgtt cgcgatgcgc cgcttcctcg aggtctgagg gtccttggcc
     3601 ggctcgcggc catccggtat catcatcgtc ccctctgaac cggatccgat ctgatgaagc
     3661 gactcttcct cgtggtgtgc gctctggccg tggcccttcc tctgccggcg gcggaggcgg
     3721 gccccgagca gcagctcgac cgcatccagg acgagctgga gcgccagcgg gagctgctcc
     3781 aacggacgaa cgaggagcgc gagggcctcc tcgacgagat ctcggatgcc gaccgacgtc
     3841 ggggtgacct caccgcgaag atcgaggccc tccagggcga cctggctctg gccgaggagc
     3901 gcctccagga ggcccaggcc tcgctcgatc tggcccgggc ctcgctccgc cgctggacca
     3961 accgcctgga gggcgcccgg gaccggctga cggcccagcg cgacacgctc tcctcccgcg
     4021 cggcggccgc ctacatgatg ggcgccgggg gcctggtgga ggtgatcctg ggggccgagg
     4081 acctccggtc cctgaccgac cgcctcgagt acgtgcgcag cgtcatggac agcgactcct
     4141 acctgctcag ctcgatccag gccacgcggg tgcaggtggc cgagcggcgg gaccacgtcg
     4201 cggaccagga gtcggtcctc tcggagcatc gtgacgcgat ccggcaggag gtcgagcgga
     4261 tcgcgcagct gaaggcggag caggaggccc tccgcgccga ggtggacgcg gcgatcgccg
     4321 agcgggagcg gctgctggag ggggtcgagg agaaccgagc tcagcacgtc aaggcggtca
     4381 aggagctcga ggccgagtcg gccaggatcg ccggcctgat ccagggcggc ggatcgtcgg
     4441 gctccggcaa cccgaacgcc cagctgttct ggccggcgcc cggctcgatc acctcgggct
     4501 tcgggtggcg ggtccacccg atcttcggca cgcg
//
LOCUS       DASUTW010000011         3778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_910946, whole genome shotgun sequence.
ACCESSION   DASUTW010000011 DASUTW010000000
VERSION     DASUTW010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910946"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VF058_00425"
     CDS             <1..460
                     /locus_tag="VF058_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206801.1"
                     /translation="NSALDEQGPSYVEIDGGAQLYFSRSSAVVLGDIFVSTRIGDGAF
                     GPAAAVAELNEATANDIQPNVRKDGREVVFSSNRAGGFGAQDIWVSTRESDADPWATP
                     VNLGGNVNSGAAETRPSLSWDARELLFGRAPGPEGMSDIYLSTRERLTGG"
     gene            complement(582..959)
                     /locus_tag="VF058_00430"
     CDS             complement(582..959)
                     /locus_tag="VF058_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206802.1"
                     /translation="MMGRTVAIVLLLVAAACGGSDAGQALVGTWVGTGDVPEEVTMIL
                     MEDGSFTWSIMDISGTWEADDTSLTLAFPEDSPFCAGGELLWEYQLDGDTLTSDVVGG
                     DCPEPVDVGPPSPDWRFERRGPA"
     gene            1171..2820
                     /locus_tag="VF058_00435"
     CDS             1171..2820
                     /locus_tag="VF058_00435"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6206803.1"
                     /translation="MRLRRLVVALAAAIAGVVPAADLGRSQPSPDQDALIRAACSVPT
                     RLLARTLRGHREDRASEIQLLPQAPAFIGHGGLPHSGPWDFVTEVPMLWYGPGFIRSR
                     GASDRPVSLADVAPTQAELLGFHLPDAEGAALEEILVPRAERSEPPRLIVTLVWDAAG
                     GNVLREWPDAWPNLRGLIPQGAWFGRANIGSSPPSTAQIHATIGTGVFPRTHGITAHR
                     MRVGGGPLVTPWKPGPNLLLRPTLADLYDRARRNRPLVGTIGSGPVHLGMMSHGAMWG
                     GGDRDVAALRVDQSAETLGAEAHRWQLDPTTAPWYRHLEYLNDLPPLSDYLPEVDRSD
                     GRSDGRWHGHTMEELDEGAYSPARVPFQTRAVREVIEREGFGVDEVTDLLFLNYKLLD
                     EIGHRFSMNGVEMRDSLLAQDADLPRLIRLLDETVGRGRWVMVLTADHGHTPDPAVTG
                     HFMISPVKVGGAVLDRFDRDGDDRRLVDKTLPTQLFIDTAELRDNGHTLEEVSAFVLS
                     LTKARVAAEGIAYAPSEARDRVFAAVLPSAMLDELPCLRDG"
     gene            2813..3238
                     /locus_tag="VF058_00440"
     CDS             2813..3238
                     /locus_tag="VF058_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206804.1"
                     /translation="MDSARRVGRWVAAAAVGAALLVSCEAPAEPGSPSPSPTVAAAPG
                     RYTFSLSGLAASLDLQGTSGELEVRNGTDRPLGAPGIVWLGAVDGREREAIVGGAEPI
                     PPGETVTVEVDFPAGFRPQRAGLIILTLDSVRWGALNPP"
     gene            complement(3472..>3778)
                     /gene="ssrA"
                     /locus_tag="VF058_00445"
     tmRNA           complement(3472..>3778)
                     /gene="ssrA"
                     /locus_tag="VF058_00445"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          568 a         1349 c         1311 g          550 t
ORIGIN      
        1 caacagcgcc ctggacgagc agggtccctc ctacgtggag atcgatggag gggcgcagct
       61 gtacttctcg cggagctcgg cggtggtgct cggcgacatc ttcgtcagca cgaggatcgg
      121 ggacggggcc ttcgggccgg ctgctgctgt ggcggagctg aacgaggcga cggcgaacga
      181 catccagccc aacgtgcgca aggatgggcg agaggtcgtc ttctcctcga accgcgcggg
      241 cgggttcgga gcccaggaca tctgggtctc gacccgggag agcgacgcgg atccctgggc
      301 gacgccggtc aacctgggcg gcaacgtgaa cagcggcgcg gccgagacgc ggccctcact
      361 gtcgtgggat gcccgagagc tgttgttcgg gcgggcgccg gggcctgagg ggatgtccga
      421 catctacctc tcgacccggg agaggctgac gggcggctga aggcaacctg agcacggagt
      481 cagagagggc gggcccccca ggggcccgcc ctctcaacgt gtgggggtgg cgcgaatcga
      541 acccaagtga ccgagcccgg cgctgtcacg gcgggcgcga gctatgcagg gcctcggcgc
      601 tcgaatcgcc aatccgggga cggcggcccc acgtcgaccg gctcggggca gtcccctccg
      661 accacgtccg aggtcagcgt gtcaccgtcc aactggtact cccacaggag ctcgcctccc
      721 gcacagaacg ggctgtcctc ggggaaggcc agggtcagtg aggtgtcgtc cgcctcccat
      781 gtgccgctga tgtccatgat ggaccaggtg aaggacccgt cctccatcag gatcatcgtg
      841 acctcctcgg ggacgtcccc tgttcccacc cacgtgccga cgagagcttg gcccgcatcg
      901 gagcccccac acgcggccgc caccagcagc agcacgatcg cgaccgtgcg ccccatcatc
      961 tcccacctcc cgttcgagac gaaacaagca tatccaggga cgccaccgca acgccaggga
     1021 gagcctcggg agtcgaacct cgggggacga tgagcaggcg ctcgtcgtgg tgtgcatcgg
     1081 tcggggccca cccctcttca acgtgagcgg cccggacaac ggctagacga gctctacagg
     1141 gaacgcggga ccttctgcct atactgcccc gtgcgcctcc gacgtctcgt cgtcgccctc
     1201 gcggccgcga tcgcgggggt cgtccctgcc gcggacctcg gccggtccca accctccccg
     1261 gatcaggacg ccttgatccg ggccgcctgc tccgtcccca cgaggctgct cgcccgaacg
     1321 ctccgcggtc accgcgagga tcgggcatcc gagatccagc tcctccccca ggcgcccgcc
     1381 ttcatcgggc acggagggct cccacattca ggcccctggg acttcgtcac cgaggtcccg
     1441 atgctctggt acgggcccgg gttcatccga tcgagggggg catccgaccg gcccgtgtcg
     1501 ctggccgacg tcgcgcccac gcaggcggag ctcctcggct tccacctgcc cgacgcggag
     1561 ggagcggccc tcgaggagat cctcgttccc cgagccgagc ggtcggagcc accccggctt
     1621 atcgtcacgc tggtctggga cgcggccggc gggaacgtcc tccgcgagtg gcccgacgct
     1681 tggcccaacc tgcgaggcct catcccccag ggagcctggt tcggacgcgc gaacatcggc
     1741 tcctcgccgc cgagcacggc gcagatccac gccaccatcg ggaccggggt gttcccccgg
     1801 acccacggga tcacggctca ccgaatgcgg gtcggcggcg gcccgctggt cacgccgtgg
     1861 aagccgggcc cgaacctcct gctccggccg acgctcgccg acctctacga tcgggcgaga
     1921 cgcaaccgcc cgctggtggg cacgatcggg agcggcccgg tccacctcgg gatgatgagc
     1981 catggggcga tgtggggagg cggcgaccgc gacgtggctg ccctccgcgt ggatcagagc
     2041 gccgagacgc tcggggccga ggcccaccgc tggcagctcg acccgaccac cgcgccgtgg
     2101 tatcgccacc tcgagtacct gaacgatctc ccgccgctct cggactacct ccccgaggtc
     2161 gaccgctccg acgggcggag cgacggtcgg tggcacggtc acacgatgga ggagctggac
     2221 gagggcgcgt actcgccggc tcgggtgccg ttccagacac gcgccgtccg cgaggtgatc
     2281 gagcgcgagg ggttcggggt cgacgaggtg accgatcttc tgttcctcaa ctacaagctg
     2341 ctggacgaga tcggccaccg attctcaatg aacggcgtcg agatgcggga ttcgctgctg
     2401 gcccaggacg ccgacctccc tcggctcatc cgccttctcg acgagaccgt agggcgcgga
     2461 cgatgggtga tggtgctgac ggcggaccac ggacacacgc ccgacccggc cgtgacgggc
     2521 cacttcatga tctctccggt gaaggtgggg ggcgccgtcc tcgataggtt cgaccgcgac
     2581 ggggacgacc ggcgcctcgt ggacaagacg ctcccaaccc agctgttcat cgacactgcc
     2641 gagctccgcg acaacgggca caccctcgag gaggtctcgg ccttcgtcct ctcgctgacg
     2701 aaggctcggg tcgccgcgga ggggatcgcc tacgctccgt cggaggcccg agaccgcgtg
     2761 ttcgccgccg tcctcccgtc ggccatgctc gacgaactcc cctgcctccg cgatggatag
     2821 cgcgcggcgg gttgggaggt gggtcgccgc ggcggcggtc ggggccgcgc tgctcgtctc
     2881 gtgcgaggcc ccagcggagc ccgggtcacc ctcgccctcc ccgacggtcg cggccgcgcc
     2941 cggccgctac acgttctcgc tctcgggtct ggccgcctcg ctcgacctcc aggggacgtc
     3001 gggagagctc gaggtccgca acggcacgga ccggccgctg ggcgctccgg ggatcgtgtg
     3061 gctcggagcc gtcgacggca gggagcgcga ggcgatcgtc gggggcgccg agccgatccc
     3121 cccgggggag acggtcaccg tggaggtcga cttcccggcg ggattccggc cccagcgcgc
     3181 ggggttgatc atcctcacgc tggactcggt gcggtggggg gccctcaacc cgccgtagcg
     3241 gcggtgaccc gcgtgccgtt ctcctcgaca tcccgagaga tcaaacggga ccccgctcac
     3301 gtcggccctg cgatccccag cattgagaga gagatcggtg accgacccga tcgcggacgt
     3361 cctcggtgac taccggacga agggcgatga cccgcgtcga acaccgtgac cggcggagtt
     3421 cttcgaagta ttcgagccgc tcttcgcgcc cgagttcgaa taagcctctg aaagtggagg
     3481 tggcgggaat cgaacccgcg tccccgagtg accgaccggg gcttctccga gcgcagcctg
     3541 cggtgtgtct catccggcgg ctcccacagg cagaggaccg ccggaccagc ctcgctgcga
     3601 tgtccctccg gggccgcgag gcgcaaccct ggaggtgagt ctgcttacga cgcccggccc
     3661 gcggccgcag acgctccgcg gcggacggct gctatttagg cagcaagcgc gagttcagaa
     3721 tcggcgctta tcttggtccc ggctctttaa cgaggactcc ggggacctcg gctcgctt
//
LOCUS       DASUTW010000012         4318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_911912, whole genome shotgun sequence.
ACCESSION   DASUTW010000012 DASUTW010000000
VERSION     DASUTW010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911912"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VF058_00450"
     CDS             complement(<1..280)
                     /locus_tag="VF058_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206805.1"
                     /translation="MIPQDVWDRTGGIDMERAWLRGELPRTPLSNLLGTEVRRVEDGT
                     LTLAMPATPWFANTGGTFYGGSVGAVRRLHYPRSHPLLRPGRNLLGHAR"
     gene            complement(277..723)
                     /locus_tag="VF058_00455"
     CDS             complement(277..723)
                     /locus_tag="VF058_00455"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX6206806.1"
                     /translation="MTGIDLVEFGIGTATFRLPASGWLHSPAGLITGGAMAFVADGAL
                     GTAILSTLPPQRILATSDLALNFLRPPADGSTATIARGRVIQVGRSQALSEAAVEDDT
                     GRFLAHATSRCVISEVPGPLPKLAREPLPGRTTLASTLSGVPRRVR"
     gene            complement(985..1842)
                     /locus_tag="VF058_00460"
     CDS             complement(985..1842)
                     /locus_tag="VF058_00460"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX6206807.1"
                     /translation="MKTDITILSPHLDDGVLSLGGLIGREAAAGRSVEIVTCYTAGPP
                     LEKVTPERRVFADYTTRRAEDERALAVLGASRHWLDLRERALRRPPLRRPMHAFRTPP
                     RMEDFAELPAIGLVIAELLNRGADVYAPLGVGNHVDHVEVALAALGEMLKRGAFERVR
                     FYEDSYALSGACRRKHFVARRHTWRRFNAPAWASPRVGALLLAIALSARGPRLDDYMT
                     EASRLEWSCEPAPMSTAEEDRKLAAVAEYRSQVKTLGGIRRVRPFLRRGHRVLGGEPI
                     WRCRPGGMQ"
     gene            complement(1839..1988)
                     /locus_tag="VF058_00465"
     CDS             complement(1839..1988)
                     /locus_tag="VF058_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206808.1"
                     /translation="MGAGRTMERLTAQDLMMVRPEDLGWPQDIGALAILEGTDLLNPE
                     GRAAS"
     gene            complement(1992..2459)
                     /locus_tag="VF058_00470"
     CDS             complement(1992..2459)
                     /locus_tag="VF058_00470"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX6206809.1"
                     /translation="MRAREEVRSPPKSTPWVNRAMSRLLRSPLSRLVDRGIVLLTVYG
                     RNTGHPYTFPVQYVQQGEVLWVYVGHGEQKTWWRNLTDDGPVEVLLRKRLRTGRGLAL
                     AHDAAPEAVEEGLRRYADRFPGAARRLRIPTVDDETIARAAARTVIVRITLEE"
     gene            complement(2463..2939)
                     /locus_tag="VF058_00475"
     CDS             complement(2463..2939)
                     /locus_tag="VF058_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX6206810.1"
                     /translation="MPKIKDAASEFLAKRKIAVTGVSRDPKAHGSNVVYQRLRDRGYV
                     VFAVNPNAQEVEGDRCYPDLGSIPGGVDAVVIGTRPDRAEATVRECAELGIKHVWMHR
                     SFGKGSVSEGATEYARDEGIEVIDGGCPCMFEPTSDPGHKLMRFVFTLAGNVPREV"
     gene            3106..3369
                     /locus_tag="VF058_00480"
     CDS             3106..3369
                     /locus_tag="VF058_00480"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX6206811.1"
                     /translation="MPRFAYTYLMKDEPERVRAAAPGHAAYWHDLGPPGYLGGPFGDR
                     SGGLITFEAEDERAARRLVDEDPFVRQDLLEASWLKEWAPEPP"
     gene            3584..3811
                     /locus_tag="VF058_00485"
     CDS             3584..3811
                     /locus_tag="VF058_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206812.1"
                     /translation="MVVVAGTAGLAAVSTAVSLTFGGVASLVAGVVLIGQIMGEIVLL
                     KQPGRPSRIEVSYFIVGFVMAVLSLLVAVLR"
     gene            4169..>4318
                     /locus_tag="VF058_00490"
     CDS             4169..>4318
                     /locus_tag="VF058_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020489920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6206813.1"
                     /translation="MSRIRRFDHVGITVADLDTVTAFFVGLGFEVEGRTFMEGEFVDT
                     VIGIPN"
BASE COUNT          694 a         1462 c         1462 g          700 t
ORIGIN      
        1 gtcgggcgtg gcccaggagg ttccggccgg ggcggaggag tggatggctc cgtggatagt
       61 gtagtcggcg aacagcgccc acggagccgc cgtagaacgt ccctccagtg ttggcgaacc
      121 acggggtggc cggcatcgcc agggtgagag tcccgtcctc gacccggcgt acctccgtcc
      181 ccagcaggtt ggagagtggg gtacggggaa gctctccccg cagccaggcc cgctccatgt
      241 cgatcccgcc cgtgcggtcc cacacgtcct gggggatcac ctcaccctcc gggggacgcc
      301 cgaaagggtg gaggccaggg tagtccggcc aggcaacggc tccctcgcga gcttcggcaa
      361 gggtcccggt acctcgctga tcacgcaccg ggaggtagcg tgggccaaga aacgccccgt
      421 gtcgtcctcc acggcggcct cggagagcgc ctgtgaacgg cccacctgga tcacccggcc
      481 gcgggcgatg gtcgcggtag aaccgtcggc ggggggccgg aggaagttca gggcgaggtc
      541 cgaggtggcg aggatcctct gcggcgggag ggtcgacagg atggcggtcc ccaaggcccc
      601 gtctgcgacg aaggccattg ccccgccggt gatcaggccc gccgggctgt ggagccaccc
      661 gctcgccggc agccggaagg tcgccgttcc tatcccgaac tccacgagat cgatcccggt
      721 cacgtaccag agaggtccgt agggcagctc acgtcgccgg aaccgctcga tctgatcgaa
      781 ccccgtcagc gtcaggaggc ccgggtgctc aagtacgcga accgcccgcg gaccggctcc
      841 cgaacggagt gggatctcgc atcattcgga ggaacggtga tcacgtctca tccgtccctt
      901 cgtcgaagac gatccccgag gaggccatgc gccagagccc ggcagcacgg cggggactgg
      961 cctggcgggg cgcactgacc gtggtcactg catgcctccg ggacggcatc tccagatcgg
     1021 ctcgccgccg agcactcggt ggccgcggcg gaggaacgga cgtactcggc ggatcccccc
     1081 caaggtcttg acctgggagc gatactcggc gaccgcggcg agcttgcgat cctcctcggc
     1141 ggtggacatc ggcgccggct cgcaactcca ttccagacgt gatgcctccg tcatgtagtc
     1201 gtcgagccgg gggccgcgcg cggagagggc gatcgccagc aggagcgccc cgacacgggg
     1261 gctggcccac gctggcgcgt tgaaccggcg ccacgtgtgg cgacgcgcga cgaagtgctt
     1321 gcgccggcat gcgcccgaca gcgcgtatga gtcctcgtag aaccggaccc gttcgaaagc
     1381 accgcgcttg agcatctctc ccagcgccgc gagcgcgacc tcgacgtggt ccacatggtt
     1441 gcccaccccc agcggtgcgt acacgtccgc cccgcggttg agtagctcgg cgatcacgag
     1501 cccaatcgct ggcagttccg cgaagtcctc catgcgtggc ggcgtgcgga acgcgtgcat
     1561 cggccgtcgc aacggcggcc tgcgcaaggc gcgttcgcgc aggtccagcc agtgccgcga
     1621 agcgcccagc accgcgaggg cccgctcgtc ctccgctcgt cgggtggtgt agtcggcgaa
     1681 cacccgacgc tccggcgtga ccttttccag tggcggaccg gccgtgtagc aggtcactat
     1741 ctcgacgctg cgcccggcgg cggcctcccg accgatgagg cctccgagcg atagcacgcc
     1801 gtcgtcgagg tggggcgata ggatcgtgat gtcggtcttc acgatgccgc ccgaccttct
     1861 gggttgagga ggtcggtgcc ctccaggatg gccagggctc cgatgtcctg cggccagccg
     1921 aggtcctcag gccgtaccat catcaggtcc tgagcggtca ggcgctccat cgttcttccg
     1981 gcccccaccg atcactcctc gagcgtgatc cggacgatga cggtcctggc agccgctcgg
     2041 gcgatggtct catcgtctac ggtagggatc cgcagacgcc tggccgcgcc ggggaaccga
     2101 tcggcgtacc tgcggaggcc ctcctccacc gcttcaggtg ccgcgtcatg ggccagggcg
     2161 agccctcgcc cggtccggag gcgttttcgg agcagaacct cgacgggacc atcatcggtg
     2221 aggttccgcc accaggtctt ctgctccccg tgcccgacgt agacccacag cacctcaccc
     2281 tgttgcacgt actgcacggg gaaggtgtac gggtgcccgg tgttgcgtcc gtagaccgtc
     2341 agcaggacga tcccccgatc gaccaggcgg gagaggggcg accgcagcaa ccgactcatg
     2401 gcccggttca cccacggcgt ggacttcggt ggactccgga cctcttctcg ggcgcgcatc
     2461 tctcacacct cccgggggac gttgcccgcg agagtgaaga cgaagcgcat cagcttgtgg
     2521 ccggggtccg aggtgggctc gaacatgcag gggcatcctc cgtcgatcac ctcgatcccc
     2581 tcatcgcggg catactcggt cgccccctct gagacgctcc ctttcccgaa ggaccggtgc
     2641 atccacacgt gcttgatccc tagctccgcg cactctcgca cggtagcctc cgcccggtcc
     2701 ggcctcgtgc cgatcaccac ggcgtccacc ccgcccggga tcgacccgag atccgggtag
     2761 caccgatcac cctccacctc ctgtgcattc gggttgacgg cgaagactac gtagcctcgg
     2821 tcccgcagcc gctggtacac gacgttgctc ccgtgcgcct tcgggtcccg ggagaccccg
     2881 gtcaccgcga tcttccgctt ggctaggaac tccgaagcgg cgtccttgat cttcggcatg
     2941 gcgccccctc cccttccatt caagtcacca ggcttcccat cccgctgagc tggaagcatg
     3001 agtcgatggg ccctattgca gggggaccct cgtccctagc ccgcccggct cgtgcggatc
     3061 caccctccgc cagaatccag ctctggggaa ggagggagca gatcgatgcc tcggttcgcc
     3121 tacacctact tgatgaagga cgagcccgag cgggtccgcg ccgccgctcc cggccacgcc
     3181 gcctactggc acgacctggg gccccccggc tacctcgggg gaccattcgg agaccggagt
     3241 ggcgggctga tcaccttcga ggccgaggat gaacgcgcag cgcgccggct cgtcgacgag
     3301 gacccgttcg tccggcaaga cctcctcgag gccagctggc tcaaggaatg ggcgcctgag
     3361 cccccgtgaa gcaggatgca cgtagggaga cgaggtgcga gatgtaagag ctagcgagtg
     3421 cggatatcga ccggtcggga tccgtgtcct cctgccgtga cgtggatcga atagggggta
     3481 atgactcgtg gactggcttc ggatcggaat cggtggaggt gctccctcca ccggctgagg
     3541 gggaacaccc tttcgtatct gcttcatccc tggcctgatc ctggtggtcg tagtcgccgg
     3601 taccgccggc ctcgccgctg tctcgaccgc ggtcagcctt acgttcggcg gcgtggcctc
     3661 gctggtggcg ggcgtcgtcc tgatcggcca gatcatgggg gagatcgtgc tcctgaagca
     3721 gcccggtcgg ccgagcagga tcgaggtctc ctacttcatc gtcggcttcg tgatggccgt
     3781 gctgagtctg ctggtggctg tgctgaggtg agaagcgagg aagctgccgc gaggatcgtc
     3841 ggtatgaaga ggagatagga gatcatcccc agtacatcca aggtcaggag ttcagccagc
     3901 cagttgtcgc ggaagagctc gaggtgttcg agggcggtgg gcggtctcgg gaagccagcg
     3961 cagacgatga tctcgcccac cagcaggacg gcgatgatca gggctgtgac gccgccaagc
     4021 ttgtacagac ctcgccaagt ggttgccgac ctcagggaca accgccatcc gccacaaacc
     4081 ctgtagggtc acggacgtgg gacttccgta ggcaagcggc ggcaacgcga gtctgacctg
     4141 acggaccaac acaggaggat ggcaccccgt gtcgcgaatt cgacgcttcg accacgtcgg
     4201 catcacggtc gcggacctcg acacggtgac ggcgttcttc gtcgggctgg gtttcgaggt
     4261 cgagggcagg acgttcatgg aaggcgagtt cgtcgacacc gtgatcggca taccgaac
//
LOCUS       DASUTW010000013         5793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1300967, whole genome shotgun sequence.
ACCESSION   DASUTW010000013 DASUTW010000000
VERSION     DASUTW010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300967"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..916
                     /locus_tag="VF058_00495"
     CDS             26..916
                     /locus_tag="VF058_00495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX6206814.1"
                     /translation="MAGGQERILRGRIRSVQATKKITRAMELIAASRIVRAQHRVLAA
                     RPYADLLTQAMEDVASNAGSLDHPLLEERPNPERAAVFVVTSDRGLAGAYNANVLRRA
                     ERLAGRLRGEGVAPTLYVAGRKGQSYFRFRGAEVEDTWTGVSEVPSYADAERIARTLI
                     GAFAQHRVDAVHAVYTDFRSAFTLRPIDKRFLPIAPEEVAGQGGAPSAEYIFEPPPEQ
                     LLDGLLPRYVTAKIFHAMMESAASELASRRRAMKAATDNAEELIRQLTRVANQARQAE
                     ITTELMEVVGGAEALKQARG"
     gene            920..2356
                     /gene="atpD"
                     /locus_tag="VF058_00500"
     CDS             920..2356
                     /gene="atpD"
                     /locus_tag="VF058_00500"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX6206815.1"
                     /translation="MAESGNGRPDGRVIQVIGPVVDVEFPPEALPEITTALELDRTIG
                     DETDMIVCEVAQHIGASVVRAIAMKPTDGVTRGTEVRNTGSAISVPVGPKVLGHVYNV
                     TGEPLDTDSVEYEERWPIHRDPPKFEDLEPRTEMFETGIKVIDLLEPYVEGGKIGLFG
                     GAGVGKTVIIQEMIYRVAEQHGGVSIFAGVGERTREGNDLILEMTKTGVIDKTALVFG
                     QMDEPPGVRLRVGLAALTMAEYFRDVQRQDVLLFIDNIFRFVQAGSEVSTLLGRMPSA
                     VGYQPTLADEMGELQERITSTRGRSITSIQAVYVPADDITDPAPHTTFAHLDATTVLS
                     RQISELGIYPAVDPLDSTSRILDPRYIGEEHYQVARTVQEILQRYRDLQDIIAILGID
                     ELSEEDKVVVSRARKIQRYLSQPFFVAEQFTGIPGKYVSREDTIRGFRMITEGELDHL
                     PEQAFYMVGDIEEAIEQGEKLAKVGAEE"
     gene            2357..2761
                     /gene="atpC"
                     /locus_tag="VF058_00505"
     CDS             2357..2761
                     /gene="atpC"
                     /locus_tag="VF058_00505"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEX6206816.1"
                     /translation="MAKPLRVAVVSPEREVYAGTARMVVAKGVAGDVGILPDHAPMLV
                     ELAIHAVRVIVEEGREDVILVDGGFLNVTPSEDETRVDVLAEHAELASEIDATAAAAR
                     AEELKRRLEDEEDASLRAELARAMMRAEHGRG"
     gene            complement(2825..4174)
                     /locus_tag="VF058_00510"
     CDS             complement(2825..4174)
                     /locus_tag="VF058_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206817.1"
                     /translation="MRRLLRILVPATFAVAMVGASLPAGAVQPPHEGTDNIKHMGNVP
                     SSGFRDSDLAFKGRLAFAGNYGGFRVIDVSEPDNPQVITNFPCNGAQGDVSVYGDLLF
                     QSVDAPQSHEGCDSTTVNAATPGMFEGVRVFDISDVENPVHIDSIHTDCGSHTHTLVP
                     DPDSNRVVIYVSSYPLGGAALGPNCVRAEDGGGHSKISVIEVPLDDPTANTVTPYHLD
                     PGTEWATYLGAFTLRACHDISVFTGSVNRAAAACLSEGQIWDISDPLNPQFLYRYDNP
                     NIRPENIDLFHSASFSWDGEVVAFGDESGGGGAARCVDPDDNQGRIWFVNLASGEELA
                     SYKIPRSEAGTCTMHNFNFVPTKRGQKVLVSSSYTGGTTVVDVDALLDGASEADAEIG
                     WFKGEGDNSWSSYWYNGRIYSHGVRGLDVLLLSDKARASAQKQAYSNPQTQEQLIGG"
     gene            4385..5656
                     /gene="murA"
                     /locus_tag="VF058_00515"
     CDS             4385..5656
                     /gene="murA"
                     /locus_tag="VF058_00515"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018639320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX6206818.1"
                     /translation="MDRLVVTGGRRLQGAVRISGAKNSALKLMAASLLAEGMTAVHNV
                     PDILDVRTMRDVLEHLGVRVVRKDRSIAMDTSGPLGTDTPYELVRRMRASILVLGPLL
                     ARLGKARVAMPGGCNIGTRGIDLHVRGLERMGATFEAEHGYLEARTDGLRGAVVSLDF
                     PSVGATENLIMAAVAARGTTVVENAAREPEIADLAAFLTAMGARIEGVGTPTIEIEGV
                     NAFHPAEHTVIPDRIEAGTFAIAACATQGDVLLRDARADHLDLVLAKLEETGAAIDAG
                     PEGIRVRMERRPRAADLVTLPYPGFPTDLQPPMLALLARAEGTSIVTENVFESRYMFV
                     DELNRMGADIRTEGHHAVVRGVERLSAAPVRALDLRAGAAMIVAGLAADGSTEIADVH
                     HVDRGYEEIDVRLASLGAAIERRTLAPALST"
BASE COUNT          895 a         1966 c         2079 g          853 t
ORIGIN      
        1 cagcggacga ggagcccgtg acgtaatggc gggtggtcag gagcggatcc ttcgtgggcg
       61 catccgcagc gtccaggcga cgaagaagat cacgcgcgcc atggagctga tcgccgcgtc
      121 acgcatcgtc cgggcgcagc accgcgtgct cgccgcgcgg ccgtacgcag acctcctgac
      181 ccaggcgatg gaggacgtgg cgagcaacgc cggctccctg gaccaccccc tccttgagga
      241 gcggccgaac cccgagcgcg ccgcggtctt cgtggtgacc tccgaccggg ggctcgcggg
      301 tgcctacaac gccaacgtgc tccgccgagc cgagcggctc gccggacgac tgcggggcga
      361 gggcgtggcc ccgaccctct acgtcgccgg gcggaaggga cagtcctact tccggttccg
      421 gggcgccgag gtcgaggaca cctggaccgg cgtgagcgag gtgccctcct acgccgatgc
      481 cgagcgcatc gcgcggaccc tgatcggtgc cttcgcccag catcgcgtcg acgccgttca
      541 cgccgtctac acggacttcc gctcggcgtt cacccttcga ccgatcgaca agcgcttcct
      601 ccccatcgcg ccggaggagg tcgccggcca gggaggagcg ccgtcggccg agtacatctt
      661 cgagccgccc ccggagcagc tgctcgacgg gctgctgccg cgctacgtca cggcgaagat
      721 ctttcacgcg atgatggagt cggccgcctc cgagctcgcc tcgcggcgcc gcgcgatgaa
      781 ggccgccacc gacaacgccg aggagctgat ccgccagctc acccgggtcg cgaaccaggc
      841 ccgccaagcg gagatcacca ccgagctgat ggaagtggtc ggaggggccg aggccctgaa
      901 gcaagcgaga ggatgagcga tggctgagag cggcaacgga aggcccgatg ggcgcgtgat
      961 ccaggtgatc ggcccggtcg tggacgtgga gttcccgccc gaggcgctgc cggagatcac
     1021 cacggcgctc gagctcgacc gcacgatcgg ggacgagacc gacatgatcg tctgcgaggt
     1081 cgcccaacac atcggggcgt cggtggtgcg cgcgatcgcg atgaagccca ccgacggcgt
     1141 cacccgcggc accgaggtcc ggaacaccgg ctctgcgatc agcgtcccgg tggggccgaa
     1201 ggtgctgggg cacgtctaca acgtgaccgg ggagcctctc gacaccgatt cggttgagta
     1261 cgaggagcgg tggccgatcc atcgcgaccc gcccaagttc gaagacctcg agccgcggac
     1321 cgagatgttc gagaccggca tcaaggtcat cgacctgctg gagccatacg tggagggcgg
     1381 gaagatcggc ttgttcggcg gtgcgggcgt gggcaagacg gtcatcatcc aagagatgat
     1441 ctaccgggtc gccgagcagc acggcggcgt ctcgatcttc gcgggggtgg gggagcggac
     1501 ccgcgagggc aacgacctga tcctggagat gaccaagacg ggggtcatcg acaagaccgc
     1561 gctcgtgttc gggcagatgg acgagccccc cggcgtgcgc ctgcgcgtcg gcctggccgc
     1621 cctgaccatg gccgagtact tccgcgacgt ccagcggcag gacgtgctgc tgttcatcga
     1681 caacatcttc cggttcgtgc aggcggggtc cgaggtctcc acgctcctcg ggcgcatgcc
     1741 gagcgccgtc gggtaccagc ccacgctggc cgacgagatg ggcgagctgc aggagcggat
     1801 cacctcgacg agggggcggt cgatcacgtc gatccaggct gtctacgtgc cggccgacga
     1861 catcaccgac ccggctccgc acaccacgtt cgctcacctc gacgcgacga cggtgctctc
     1921 gaggcagatc tcggagctcg ggatctaccc ggcggtggac cccctcgact cgacgtcgcg
     1981 gatcctcgac cctcgctaca tcggcgagga gcactaccag gtggcgcgca cggtccagga
     2041 gatcctccag cggtacaggg acctccagga catcatcgcg atcctgggga tcgacgagct
     2101 ctccgaagag gacaaggtcg tcgtgtcgag ggctcggaag atccagcggt acctctccca
     2161 gccgttcttc gtcgccgagc agttcacggg gatcccgggc aagtacgtct cgagagagga
     2221 cacgatccgc gggttcagga tgatcaccga gggcgagctg gatcacctac ccgagcaggc
     2281 cttctacatg gttggggaca tcgaggaggc gatcgagcag ggcgagaagc tggccaaggt
     2341 gggcgcggag gagtaggtgg cgaagccgct ccgcgtcgcc gtggtctcgc ccgagcgcga
     2401 ggtctacgcg ggcacggccc ggatggtcgt ggccaagggc gtggccgggg acgtggggat
     2461 cctgccggac cacgccccga tgctggtcga gctggcgatc catgcggtcc gcgtcatcgt
     2521 ggaggaaggg cgcgaggacg tgatcctcgt ggacggaggc ttcctcaacg tgaccccgag
     2581 cgaggacgag acccgcgtgg acgtgctcgc cgagcacgcg gagctggcct ccgagatcga
     2641 tgccaccgcg gccgccgcgc gcgccgagga gctgaagcgg cgtctcgagg acgaggagga
     2701 cgccagcctc cgcgccgagc tggccagggc catgatgcgg gccgagcacg gccggggctg
     2761 acgcgtgacg tggaacgggc ccccgagggg gcccgttcgg gtcctgcgtc gtgcggtcgg
     2821 tgcgctaacc gccgatcaac tgctcctggg tctgcgggtt cgagtacgcc tgcttctgcg
     2881 cgctggctcg cgccttgtcg gagagcagga gcacgtccag accccggacg ccgtgcgagt
     2941 agattcggcc gttgtaccag tacgacgacc acgagttgtc gccctcgccc ttgaaccagc
     3001 cgatctcggc gtcggcctcg ctggccccgt cgaggagcgc gtccacgtcc accaccgtgg
     3061 tccctcccgt gtacgaggac gacacgagca ccttctggcc cctcttcgtg ggcacgaagt
     3121 tgaagttgtg catcgtgcag gtccccgcct ccgagcgagg gatcttgtag ctggccagct
     3181 cctcacccga ggcgaggttc acgaaccaga tacgcccctg gttgtcgtcg gggtcgacgc
     3241 agcgcgccgc tccgccgcca cccgactcgt ccccgaaggc cacgacctca ccgtcccagc
     3301 tgaaggacgc cgagtggaag aggtcgatgt tctccggccg gatgttcggg ttgtcgtacc
     3361 ggtagaggaa ctgcgggttc agcgggtcgg agatgtccca gatctgaccc tccgacagac
     3421 aggccgcagc ggccctgttg acggatcccg tgaacacgct gatgtcgtga caggcgcgca
     3481 gcgtgaacgc ccccaggtac gtggcccact ccgtccccgg gtccaggtgg tacggggtga
     3541 cggtgttcgc ggtggggtcg tccagcggga cctcgatcac cgagatcttg ctgtggcccc
     3601 cgccgtcctc ggcccgcacg cagttcggtc ctagggccgc cccgcccagc gggtaggacg
     3661 acacgtagat gaccacccgg ttgctgtcgg ggtcggggac cagcgtgtgg gtgtgcgacc
     3721 cgcagtccgt gtggatcgag tcgatgtgca cggggttctc cacgtcgctg atgtcgaaga
     3781 cccgaacgcc ctcgaacatc cccggcgtgg ctgcgttgac ggttgtgctg tcgcacccct
     3841 cgtggctctg cggcgcgtcc acggactgga acagcaggtc gccgtagacc gacacgtcgc
     3901 cctgtgcgcc gttgcacggg aagttcgtga tgacctgcgg gttgtcgggc tccgagacgt
     3961 cgatcacccg gaacccgccg tagttgccgg cgaacgccag cctccccttg aaggcgaggt
     4021 cggagtcccg gaaccccgag ctcggcacgt tccccatgtg cttgatgttg tccgtgcctt
     4081 catgcggggg ctggaccgcg cccgccggga ggctcgctcc gaccatggct acggcgaacg
     4141 tggccggcac gaggatcctc aagaggcgtc tcatcaggca tccctccgtc gtggttgggg
     4201 catgagtgag ctcctacgtg gtgcagggcc tgcgggcccc ttcgtccgaa gtgaccacct
     4261 ccctccgtgc gatacgtccc gagcggttgg agtctgccag gaaacgtacc gacgggggaa
     4321 tgccgtagtc aaatcgacgt aatgaggagc ggctcgcccg tcggctccgc gtagaatcac
     4381 tcgcgtggat cggctcgtgg tgaccggcgg gcggcgcctt cagggcgccg tccggatcag
     4441 cggggcgaag aactccgccc tcaagctgat ggccgcctct ctcctggccg aggggatgac
     4501 ggccgtccac aacgtgcccg acatcctcga cgtccgcacg atgcgcgacg tgctcgagca
     4561 tctcggcgtg cgggtcgtgc ggaaggaccg ctcgatcgcg atggacacct ccggcccgct
     4621 cgggaccgac accccctacg agctcgtccg ccggatgcgg gcttcgatcc tcgtgctggg
     4681 gcccctgctg gcgaggctgg ggaaggcgcg cgtggcgatg ccgggcggct gcaacatcgg
     4741 gacgcggggc atcgacctcc acgtccgcgg gctcgagcgg atgggggcca ccttcgaggc
     4801 ggagcacggg tacctcgagg cccggacaga cggcctccgc ggcgccgtcg tctccctcga
     4861 cttccccagc gtgggggcga ccgagaacct catcatggcg gcggtggccg cgcgcgggac
     4921 caccgtcgtg gagaacgcgg cgcgcgagcc cgagatcgcc gatctcgcgg cgttcctcac
     4981 ggcgatgggg gcgcggatcg agggcgtggg gacgccgacg atcgagatcg agggcgtgaa
     5041 cgccttccac ccggcggagc acacggtcat cccggatcgg atcgaggcgg ggacgttcgc
     5101 catcgcggcg tgcgccaccc agggggacgt cctgctccgg gacgcccgcg cggaccatct
     5161 cgatctcgtg ctggccaagc tcgaggagac gggggcggcg atcgacgccg ggcccgaggg
     5221 gatccgcgtg cggatggagc gccggccccg ggcggccgac ctcgtgaccc tcccgtaccc
     5281 ggggttcccc accgacctcc agccgccgat gctggccctc ctggcgaggg ccgaggggac
     5341 gagcatcgtc accgagaacg tgttcgagag ccggtacatg ttcgtggacg agctgaaccg
     5401 gatgggcgcg gacatccgga ccgagggcca ccacgccgtc gtgcgcggcg tggagcgcct
     5461 ctcggccgcc ccggtgcggg ccctcgacct ccgggcgggc gcggcgatga tcgtggcggg
     5521 cctcgccgcc gacggctcga ccgagatcgc ggacgtccat cacgtcgatc ggggctacga
     5581 ggagatcgac gtccgcctgg cgtcgctggg cgccgcgatc gagcggcgga cgctcgctcc
     5641 cgcgctttcc acgtgaggag ggcggcggcc ctcctcgcgc tcgcgaccgt cgcgctcccc
     5701 gggctggcct ccggggccca ggctccccgg gcgatcctgg tcttggggag caccgggatc
     5761 ccgtatgagg aggcgatcgc cgacccggag ctg
//
LOCUS       DASUTW010000014         3927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1431584, whole genome shotgun sequence.
ACCESSION   DASUTW010000014 DASUTW010000000
VERSION     DASUTW010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431584"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VF058_00520"
     CDS             complement(<1..608)
                     /locus_tag="VF058_00520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6206819.1"
                     /translation="MSPRIAMLTYSSKPRGGVVHALHLAEALHAQGADVHLFALGDPR
                     EGFYRLARAPHTLFPAPAADGSLEERVFAAVDALRSGLGRVAGGFDVLHAQDCIAARA
                     VLDLREKEPLGSLVRTVHHIDDFTTPALIECQQRSVVEPDRVLVVSEYWRRALAEEHG
                     IEAAVVPNGVDLARFAAPLDPEAVRDLRRRVGSADRFLFLTVG"
     gene            complement(605..2149)
                     /locus_tag="VF058_00525"
     CDS             complement(605..2149)
                     /locus_tag="VF058_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein FdrA"
                     /protein_id="HEX6206820.1"
                     /translation="MSATAVRVRPDTYVDSVILMSGARAMRDLDGVTWATAVMGTDAN
                     VDELRSRGFDDGELGSAGANDLVLAVEAGSEEEAREALDAGMRALSEAGGGDAEGPKE
                     RGPRSLQQTVADTPGINVALISVPGEHAPLEAHKALSAGLHVLLFSDNVPLDDEVELK
                     VRGRELRRFVMGPGAGTAILGGVGLGFANVVQRGPVGVVAAAGTGAQEVSSLLDRWGV
                     GVRDLIGVGGRDLSEQVEGGMTRMALEAFEADDDVETVLLVSKPPAPDVAADLVRRLG
                     EGKPAVAVLLGLTDDLDPPEGVRLARTLEEGVRLTLEILGRDGPELSRGLEERVGEAI
                     QGLDRDRTAVRGLFSGGTLCYEAMIVLSEHLEAEVRSNTPLKEGWGLPAPDGAHVCLD
                     LGEEEYTKGRPHPMLDPEPRADLIVEAAGDPSVAAILVDVVLGHGAHDDPAAVLAPAC
                     AAATDRGVPVVAYVLGTEGDPQGLGAQQKKMEQAGCLLGPTGARSALMAAAIAGRDPS
                     LAGGTP"
     gene            complement(2146..3102)
                     /gene="arcC"
                     /locus_tag="VF058_00530"
     CDS             complement(2146..3102)
                     /gene="arcC"
                     /locus_tag="VF058_00530"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011251108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEX6206821.1"
                     /translation="MMRSVVVALGGNAITREGEAGTYEEQEANARRMARAVARMVGAG
                     HRVVVTHGNGPQVGSLAIQQEEGASLVPAQPLFALGAMTQGQIGHLLGLALAPLVEAK
                     VAPIVTHVVVDPRDPAFADPTKPIGPFFDDTTARGLAAERGWTVREDAGRGYRRVVPS
                     PAPKEILESGVIRALLRQGAVVIASGGGGIPVVRRGKRLVGVDAVIDKDLSAERLGTE
                     IGADDLLMLTGVDRVALGFGTQEERPIREMRADEAETYMAEGHFPAGSMGPKIAAAVR
                     FVREGGEAAIITSPRRVGAALAGNHGTRIVPAREAPRRAAGQ"
     gene            complement(3099..3656)
                     /locus_tag="VF058_00535"
     CDS             complement(3099..3656)
                     /locus_tag="VF058_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6206822.1"
                     /translation="MEEVIAAIGPKVRQLRRQKNLSLQQLAERSGVSAAAIHKLERNG
                     MVPTITTLMKLASALNRPVSYFVDEQDPDHAVVVRSGDRKTVYTSKSGLGLESVSGPY
                     GRFFMAGAVATVEPNADSGPKPMEHPGEELVYVLSGSLCFEIDGEEHVLRRGDALHFR
                     TDRPHRWWNPGARPAKALWMALRST"
BASE COUNT          515 a         1524 c         1366 g          522 t
ORIGIN      
        1 cccaccgtca ggaagaggaa ccggtccgcc gagccgacgc ggcgccgcag gtcgcgaacc
       61 gcctcggggt cgagcggcgc ggcgaagcga gcgaggtcca cgccgttggg caccacggcg
      121 gcctcgatcc cgtgctcctc ggccagcgcc cgccgccagt actccgagac gaccagcacg
      181 cggtcgggct ccaccaccga gcgctgctgg cactcgatca gcgccggcgt ggtgaagtcg
      241 tcgatgtggt gcaccgtccg gaccagggac cccagcggct ccttctcgcg gaggtccagc
      301 acggctcggg cggcgatgca gtcctgggcg tggaggacgt cgaatccccc cgccacgcgc
      361 cccaggcccg accgcagcgc gtccacggcg gcgaacaccc gctcctcgag cgacccgtcc
      421 gcggcgggcg cggggaacag cgtgtgcggc gcgcgcgcca gccggtagaa gccctccctg
      481 gggtctccca gggcgaacag gtgcacgtcg gccccctggg cgtggagggc ctcggccagg
      541 tggagcgcgt gcaccacgcc tcccctgggc ttcgaggagt aggtgagcat cgcgatccgg
      601 gggctcacgg cgtccctccg gccagcgagg ggtccctccc ggcgatcgca gcggccatca
      661 gcgccgaccg agcgccggtg gggcccagga ggcagccggc ctgctccatc ttcttctgct
      721 gggcccccag gccctggggg tccccctcgg ttcccagcac gtacgccacc acgggcaccc
      781 ctcgatcggt cgccgcggcg caggccgggg ccaggaccgc cgcggggtcg tcgtgggctc
      841 cgtgccccag cacgacgtcc accaggatcg cggcgacgga cgggtcgccc gccgcctcca
      901 cgatcaggtc tgcccgcggc tcggggtcga gcatggggtg cgggcggccc ttcgtgtact
      961 cctcctcgcc caggtcgagg cagacgtgcg cgccgtcggg ggcggggagc ccccagccct
     1021 ccttgagcgg cgtgttcgat cggacctccg cctcgaggtg ctcgctcagc acgatcatcg
     1081 cctcgtagca gagggtgccc cccgagaaca ggccccgcac ggcggtccga tcccggtcca
     1141 ggccttggat cgcctcgccg acccgctcct ccaggccccg ggagagctcg ggcccgtcgc
     1201 gacccaggat ctctagggtg agccgcaccc cttcctccag ggtgcgggcc agccggacac
     1261 cctccggtgg gtcgagatcg tcggtcagcc ccagcaggac ggccaccgcg ggcttgccct
     1321 cacccagccg ccgcacgagg tccgccgcca cgtcgggggc cggcggcttg gagacgagca
     1381 gcaccgtctc cacgtcgtcg tcggcctcga aggcctcaag cgccatgcgg gtcatccccc
     1441 cttccacctg ctcggagagg tcacggcccc ccacgccgat caggtcgcga acgccgaccc
     1501 cccagcggtc gagcagcgag ctgacctcct gggcccccgt cccggccgcc gccaccacgc
     1561 cgaccggtcc ccgctggacc acgttggcga agcccagtcc cacgccgccg aggatcgccg
     1621 tccccgcgcc cggccccatc acgaagcgcc gcagctctct cccgcggacc ttcagctcga
     1681 cctcgtcgtc gagggggacg ttgtcgctga acagcaggac gtggaggccg gcggacagcg
     1741 ccttgtgggc ctcgagcgga gcgtgctcgc ccgggaccga gatgagcgcg acgttgatgc
     1801 ccggcgtgtc cgccaccgtc tgctgcagcg agcgggggcc gcgctccttc gggccctcgg
     1861 cgtccccgcc cccggcctcc gacagcgccc gcatgcccgc gtcgagcgcc tcccgagcct
     1921 cctcctcgga ccccgcctcg acggccagca cgaggtcgtt ggccccggcc gagcccagct
     1981 caccgtcgtc gaaccctcgg gaccggagct cgtcgacatt ggcgtcggtg cccatcaccg
     2041 ccgtggccca ggtcacgccg tccaggtccc gcatggcgcg ggcgcccgac atcaggatga
     2101 ccgagtcgac gtaggtgtcg gggcggacgc gaacggcggt cgcgctcact gcccggccgc
     2161 ccgccgcggc gcctccctcg cgggcacgat ccgggtcccg tggttgccgg cgagcgccgc
     2221 gccgacccgc ctgggcgacg tgatgatcgc cgcctcgcct ccctctcgca cgaaccggac
     2281 ggcggcagcg atcttcggac ccatgctccc ggcggggaag tgcccctcgg ccatgtaggt
     2341 ctcggcttcg tctgccctca tctcccggat cgggcgctcc tcttgcgtgc cgaacccgag
     2401 cgccacgcgg tccacgcccg tcagcatcag caggtcgtcg gcgccgatct cggtcccgag
     2461 ccgctccgcg gagagatcct tgtcgatcac ggcgtccacc ccgaccagcc tcttccccct
     2521 ccgcacgacg gggatgcctc cgccccccga ggcgatgacg acggccccct ggcggagcag
     2581 ggcccggatc acgccggact ccaggatctc cttcggagcg ggggagggga ccacccgccg
     2641 gtacccgcgg ccggcgtcct cgcggacggt ccatccacgc tccgcagcga gcccgcgggc
     2701 ggtggtgtcg tcgaagaagg gcccgatcgg cttcgtcggg tccgcgaacg ccgggtcgcg
     2761 cgggtccacc accacgtggg tgacgatcgg ggccaccttc gcctccacga gcggggcgag
     2821 ggccagcccc aggaggtggc cgatctggcc ctgcgtcatc gcgccgagcg cgaacagcgg
     2881 ctgggccggc acgagggagg ccccctcctc ctgctggatc gcgaggctgc cgacctgggg
     2941 gccgttgccg tgcgtcacga cgacgcggtg gccggccccg accatccgcg cgacggctcg
     3001 ggccatgcgc cgggcgttcg cctcctgctc ctcgtaggtc ccggcctcgc cctcgcgggt
     3061 gatggcgttc cctccgagcg cgacgacgac cgagcgcatc acgtcgaccg gagggccatc
     3121 cagagggcct tcgccgggcg ggccccgggg ttccaccagc ggtgcggacg gtcggtgcgg
     3181 aagtgcagcg cgtcgccgcg ccgcaggacg tgctcctccc cgtcgatctc gaagcacagg
     3241 gagccgctca ggacgtacac cagctcctcg ccgggatgct ccatcggctt gggaccgctg
     3301 tcggcattgg gctccaccgt ggccacggcg cccgccatga agaaccggcc gtagggcccg
     3361 gagacgctct ccaggccgag cccggacttc gaggtgtaga cggtcttccg gtcgcccgac
     3421 cgcacgacga cggcgtggtc ggggtcctgc tcgtccacga agtacgacac ggggcggttg
     3481 agggccgagg ccagcttcat cagcgtcgtg atcgtgggga ccatcccgtt ccgctccagc
     3541 ttgtggatcg ccgccgccga gacccccgac cgctcggcga gctgctggag cgagaggttc
     3601 ttctgccggc gcagctggcg gaccttcggc ccgatcgccg cgatgacctc ctccatgacg
     3661 acccctccac ttgaagattc gcgataaccc cgggtagagt tcgggtatcg gtgaacggcg
     3721 cgttgagtat agtgaataac ccgcgcgacg ggcaatcctg aggaggggcg tacatggctc
     3781 agcgctatgt ggtcgtgggc ggcggcgccg cgggcatcgg cgcggccggg gcgatcaagg
     3841 ccggggaccc cgaggccgat ctgcaggtct tcaccgagta cgaggacgcc gcctacagcc
     3901 cctgcggcat cccctacgtc cacggac
//
LOCUS       DASUTW010000015         4578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_136347, whole genome shotgun sequence.
ACCESSION   DASUTW010000015 DASUTW010000000
VERSION     DASUTW010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136347"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VF058_00540"
     CDS             complement(<1..450)
                     /locus_tag="VF058_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6206823.1"
                     /translation="MDDLRVRVRELMPQIRADLDRLVRIPSVSAEGFAPEPVRESAKA
                     TAEVLEVAGFGDVRFLEVDGAHPAVTGRIEGPQGSPTVMLYAHHDVQPAGDPAEWTSP
                     PFEPTERDGRLFGRGAADDKAGVMAHVAAVLAHDGKPPVTVTAFIEGE"
     gene            506..997
                     /locus_tag="VF058_00545"
     CDS             506..997
                     /locus_tag="VF058_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX6206824.1"
                     /translation="MAKLPLRFAGAVVLTLALLAASCGGGGRAEPAADEPVAVVRVVD
                     GDTIWVAPEQRVRLIGIDTPEVDWYGGRAECYGEEAGLFMRGLLEGERVRLEYDQEPR
                     DRYGRTLAYVYLEDGRMVNLLLVRRGYAAVSIFEPNDRYEAGLRAAEREARDVGRGLW
                     SIC"
     gene            complement(1358..2500)
                     /locus_tag="VF058_00550"
     CDS             complement(1358..2500)
                     /locus_tag="VF058_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015576331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6206825.1"
                     /translation="MDRDFGLSEDQREIVGLVREFVNERVRPQIETFEEKGEFPRELF
                     RQLGEMGLGGIPYEERYGGAVQPYLTYLTVLEELAMGSVSLAVGLSVHHLATFGVHDY
                     GSDVLKERYLPRLTSGEWLGAYALTEAGSGSDAASLATKAEREGDGWRLTGSKRFISH
                     GGEAEYYLVMARTGGEGPKGISAFVLEKGYPGFDFGKPEEKMGWNASPMRELLFDGAE
                     VPGENLVGEEGQGFTIALEALDGGRLGMAACGVGVAQAALDAAVAFAKDREQFGKPVI
                     EFQGLAFMLADMHAQVEAARRLYREAVRARDEGVGTPVSCSVAKLFATDVAMRVTTDA
                     VQVHGGYGYMREYPVERYMREAKALQIVEGTNQVQRMIIGRHLAGG"
     gene            2562..3953
                     /gene="cysS"
                     /locus_tag="VF058_00555"
     CDS             2562..3953
                     /gene="cysS"
                     /locus_tag="VF058_00555"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX6206826.1"
                     /translation="MRLYNTLTRKVEEVRPSGDGPVRLYTCGPTVYRPVHIGNLRTFL
                     LSDLVRRALELEGHEVLQVVNITDVGHMVDDSSEEALDRMELAMEDEGLGPWEIAEKY
                     TRVFLEDADKIGLLRAHEYPRATDHIAEMIEMTKILIDRGHAYALDDGSVYYDVQSFP
                     EYGKLSRNTLDKLQPGHRDLETDDRKRHHADFALWKTAQPNRLMKWGSPWGEGFPGWH
                     IECSAMSLKYLGDRFDIHTGGVDLVFPHHEDEIAQSDGATGHRVVSTWVHGGHLRVEG
                     QKMAKSTGNVYTIDEVIERGYDPLAFRLLCFQTRYRSEMNFTWEAMEAADRAVTRWRQ
                     RMAEWKDAPRGERSPEGEAFDRRFREAVADDLDMPRAVAVVGDVVSSGIPDGEKRALL
                     SSWDAVLGLDLERIAREGFEVPADVRTLVDERDAARAERDYARSDEIRDRLTEMGWEV
                     MDSAEGTRVRPLA"
     gene            3965..>4578
                     /locus_tag="VF058_00560"
     CDS             3965..>4578
                     /locus_tag="VF058_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6206827.1"
                     /translation="MDAAILTQGLTKYYGKRPGVLEVDLEVRPGEVFGFLGPNGAGKT
                     TMIRTLMDLIRPTSGRAFVLGMDAQREGVEVRRRVGYLPGDLALDEHLEARDLLGYLS
                     SFRGGVYEDEIERLASLLDAELDARIGSLSSGNRQKIGIVQAFMHRPELVVLDEPTLG
                     LDPLIQQHVYRLLAETKADGRTVFLSSHNLPEVERTCDRVGIIRR"
BASE COUNT          682 a         1701 c         1538 g          657 t
ORIGIN      
        1 ctcgccctcg atgaaggcgg tgacggtcac gggaggcttc ccgtcgtggg ccagcacggc
       61 cgcgacgtgg gccatgacgc cggccttgtc gtcggccgcc ccccgcccga acagcctccc
      121 gtcccgctcg gtgggctcga acgggggcga agtccactcg gcggggtcgc cggcgggctg
      181 gacgtcgtgg tgggcgtaga gcatcacggt ggggctcccc tgcggccctt cgatgcgtcc
      241 ggtgacggcg gggtgggcgc cgtccacctc gaggaaccgg acgtccccga accccgcgac
      301 ctcgaggacc tcggcagtcg ccttcgccga ctcccggacc ggctcggggg cgaagccctc
      361 ggccgagacc gaggggatgc gcacgaggcg gtcgaggtcg gcgcggatct gcggcatgag
      421 ctcgcggacg cgaacgcgga gatcgtccat cggcgccaca gggtatcagc gtccgtgtcg
      481 gtggggccac gtacgctggt gctcgatggc gaagctcccc ctccgcttcg cgggcgccgt
      541 cgttctgacg ctcgccctgc tcgccgcgag ctgcggcggg ggcgggcggg ccgagcctgc
      601 cgccgacgag cccgtggccg tggtccgcgt cgtggacggc gacacgatct gggtcgcccc
      661 ggagcagcgc gtccgcctga tcggcatcga cacgcccgag gtcgactggt acgggggccg
      721 ggcggagtgc tacggagagg aggcgggcct cttcatgcgg ggcctcctgg aaggcgagcg
      781 ggtccggctc gagtacgacc aggagccgcg ggaccgctac ggccgcaccc tcgcctacgt
      841 ctacctcgag gacggccgca tggtgaacct gctgttggtc cgccgaggct acgccgccgt
      901 ctcgatcttc gagcccaacg accggtacga ggccgggctg cgggcggccg agcgggaggc
      961 tcgcgacgtc ggccgcggcc tctggtcgat ctgttgactc aggcgttggc gggaggtccg
     1021 ggctcgcagg tcgcaaccac cgatccatcg tgtcgcgtct cggctgtgat ccctccgctg
     1081 ctccctgcac cgaaacccgg aggcccgacg gtgacgccac ccttccccct gcaccggatc
     1141 aggtctcctt cgcgcaactc tcctgggctg aagaccacgc gctggcctgg ctcgatccgg
     1201 acggcgcgca gctctcccgt ccgtggtggt ccctgttggt cgcgaccgcc gggccctcca
     1261 catcccgcgc ccacgatcaa cacagcgatg acgagtgaaa cggtctgccg gtgcatacac
     1321 gtccgacgca cgggggccac cgacggttcc cgagagctca accccccgcg aggtggcggc
     1381 cgatgatcat ccgctggacc tggttggtcc cctcgacgat ctggagggcc ttcgcctcgc
     1441 gcatgtagcg ctcgaccggg tactcgcgca tgtacccata gccgccatgc acctggaccg
     1501 cgtcggtcgt cacgcgcatc gcgacgtcgg tggcgaacag cttggccacc gagcacgaca
     1561 ccggcgtccc cacgccctcg tcgcgcgccc ggaccgcctc ccggtacagt cgccgcgccg
     1621 cctccacctg cgcgtgcatg tcggccagca tgaacgcgag cccctggaac tcgatcacgg
     1681 gcttcccgaa ctgctccctg tccttggcga aggccaccgc agcatcgagg gccgcctggg
     1741 ccacgcccac gccgcacgcg gccatcccca ggcgcccgcc atcgagcgcc tccagggcga
     1801 tggtgaagcc ctggccctcc tcccccacca ggttctcgcc cggcacctcg gccccgtcga
     1861 acagcagctc ccgcatcggg ctcgcgttcc agcccatctt ctcctcgggc ttcccgaagt
     1921 cgaagcccgg ataccccttc tccagcacga aggccgagat ccccttcggc ccctctcccc
     1981 cggtgcgggc catgacgagg tagtactccg cctctccccc atgggagatg aaccgcttcg
     2041 accccgtgag ccgccagccg tcgccctcgc gctcggcctt cgtggcgagc gacgcggcgt
     2101 cggagccgct tccggcctcg gtcagcgcgt aggccccgag ccactccccg gacgtcaacc
     2161 ggggcaggta ccgctccttc aggacgtccg aaccgtagtc gtggaccccg aacgtggcca
     2221 ggtggtgcac ggagagcccc acggcgaggg acacggaccc catggcaagc tcctcgagca
     2281 ccgtcagata cgtcaggtac ggctggaccg cacctccgta gcgctcctcg tacgggatcc
     2341 ctccgagccc catctcgccg agctggcgga acagctccct ggggaactcg cccttctcct
     2401 cgaacgtctc gatctgcggc cggacccgct cgttgacgaa ctcgcggacg agccccacga
     2461 tctcgcgctg atcctccgag agcccgaagt cccggtccat ggcccctcag cgtagcgaac
     2521 acgggcccga gagcccaccc agcgtctatc ctgaggtccc gatgcgcctc tacaacacgc
     2581 tgaccaggaa ggtcgaggag gttcggccgt ccggcgacgg gcccgtgcgt ctctacacgt
     2641 gcgggccgac cgtctatcgc ccggtccaca tcgggaacct ccggaccttc ctcctctccg
     2701 acctcgtccg gcgggccctc gagctggagg gccacgaggt gctccaggtc gtcaacatca
     2761 ccgacgtggg ccatatggtc gacgactcca gcgaggaggc cctggaccgg atggagctgg
     2821 cgatggagga cgagggcctg ggcccctggg agatcgccga gaagtacacg cgcgtcttcc
     2881 tcgaggacgc cgacaagatc gggctcctgc gcgcccacga gtacccgcgc gcgaccgacc
     2941 acatcgccga gatgatcgag atgacaaaga tcctcatcga ccggggtcac gcctacgccc
     3001 tcgacgacgg ctccgtctac tacgacgtcc agtcgttccc cgagtacggg aagctctccc
     3061 ggaacacgct ggacaagctg cagccgggcc accgggacct cgagaccgac gaccgcaagc
     3121 gccaccacgc ggacttcgcg ctctggaaga cggcgcagcc caaccggctg atgaagtggg
     3181 gctccccctg gggtgagggc ttccccggct ggcacatcga gtgctcggcc atgtccctga
     3241 agtacctggg cgaccgcttc gacatccata ccggcggcgt cgacctcgtc ttcccgcacc
     3301 acgaggacga gatcgcccag tcggacgggg cgaccgggca tcgggtggtc tccacctggg
     3361 tccacggcgg ccatctgcgg gtcgagggcc agaagatggc caagtcgacc ggcaacgtgt
     3421 acacgatcga cgaggtgatc gagcgaggct acgatccgct cgcgttccgg ctcctctgct
     3481 tccagacccg ctaccggagc gagatgaact tcacctggga ggcgatggag gcggccgacc
     3541 gcgcggtcac gagatggcgc cagcgcatgg ccgagtggaa ggacgcgccg cggggcgagc
     3601 gctcccccga gggagaggcc ttcgaccggc ggttccgaga ggcggtggcc gacgacctcg
     3661 acatgccccg cgccgtggcg gtggtgggcg acgtcgtctc ctcggggatc ccggacgggg
     3721 agaagcgcgc cctgctctcg tcctgggacg ccgtgctcgg cctcgacctc gagcgcatcg
     3781 cccgcgaggg gttcgaggtc cccgcggacg tccgcacgct ggtcgacgag cgcgacgccg
     3841 cccgggccga gcgcgactac gcgcggtccg acgagatccg cgaccggctg accgagatgg
     3901 gctgggaggt catggactcc gcggagggga cgcgggtgcg cccgctggcg tagcctgaca
     3961 cgcgatggac gccgcgatcc tgacgcaggg cctgacgaag tactacggca agcgtcccgg
     4021 cgtcctcgag gtggacctcg aggtccgccc cggcgaggtc ttcggcttcc tgggccccaa
     4081 cggggccggc aagaccacca tgatccgcac cctcatggac ctgatccgtc ccacgagcgg
     4141 cagggcgttc gtcctcggca tggacgccca gcgcgaaggg gtcgaggtgc ggcgccgcgt
     4201 cggctacctc ccaggggacc tcgccctcga cgagcacctc gaggctcgcg acctgctggg
     4261 ctacctctcc tcgttcaggg gcggcgtcta cgaggacgag atcgagcgcc tggcgtccct
     4321 cctggatgcc gagctggacg cccggatcgg ctcgctctcc agcggcaacc ggcagaagat
     4381 cgggatcgtc caggcgttca tgcaccgccc ggagctcgtc gtgctcgacg agcccacgct
     4441 cggcctcgac ccgctgatcc agcagcacgt ctaccggctc ctggccgaga cgaaagccga
     4501 cggccggacc gtgttcctct cctcccacaa cctccccgag gtcgagcgga cctgcgaccg
     4561 cgtcgggatc atccgccg
//
LOCUS       DASUTW010000016         3572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1561592, whole genome shotgun sequence.
ACCESSION   DASUTW010000016 DASUTW010000000
VERSION     DASUTW010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561592"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VF058_00565"
     CDS             complement(<1..995)
                     /locus_tag="VF058_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206828.1"
                     /translation="MSRVFIRTLVLIALLVGVLPGTPAGAQVADSFEIAYGDTVADGV
                     PGPGAGNIEVAGGTDAYSFEGAAGDVAIFDVLAGSNTTFLWRLEGPGGGALFDGLYVD
                     REVELPQNGTYVLTVRGFSAASTGTYSFRLLLTPPPQEFAIAFGDTVSQDVPAPGAGN
                     LEVPGAIDRYLFDATAGQVAIFDALTGNNTQVRWALHAPDGTQVFDRLYIDHQETLAQ
                     TGTYVLTMSGVNIASTGTYSFQLLQGAGTQEFTIAFGDTVSDGVPGPGAGNLEGPGAI
                     DRYLFEGTMGQVAILDALTGDNTQIAWSLHAPDGSELFDSIYVDHEVVLPQDGTYT"
     gene            1164..1889
                     /locus_tag="VF058_00570"
     CDS             1164..1889
                     /locus_tag="VF058_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206829.1"
                     /translation="MATDRSRRRRSRLPREHARKRYIEIGELVVLEQIKEDSEALDRR
                     STAVGPFARLDANAVAAREGKTRGAVNNVFGSQAAFQAETMADTLFAGDWIRQIRYPR
                     PRDFPDAEAWLDRLLTGESGRGPLHGAEPTMTYGFLWALWLSAVPYGLWSDEISRPSL
                     EEFVKRVESLERVIEEALDHFEVSLRAGTTMTDLATALASLIEGVWLNQCLTERHPSD
                     ADAPISEMLRRSGRLLWRGAVSS"
     gene            2037..2450
                     /locus_tag="VF058_00575"
     CDS             2037..2450
                     /locus_tag="VF058_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206830.1"
                     /translation="MSEQSPFPRSIYGGAFREGFVAALVDSRVEATRLVDLLSVTGIR
                     RSDVGVLPGDLALVIDGSHHPSLAVSEAAPGEAAVSERYVSGALLGDVLVAVRTPSEP
                     HARAVARILLSEGASLVYHFGPGFLRAPDMTEAAA"
     gene            2447..>3572
                     /locus_tag="VF058_00580"
     CDS             2447..>3572
                     /locus_tag="VF058_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEX6206831.1"
                     /translation="MTRPVVTVDGNEAAAYVAHKASEVIAIYPITPASPMGELADAWS
                     ARGEPNVWGSVPEVIEMQSEGGAAGAVHGALQAGALTTTFTASQGLLLMLPNMYKIAG
                     ELTPAVFHIAARTVATHALSIFGDHSDVMGARSTGWAMLASSSVQEAQDLALVAHAAT
                     LESRVPFLHFFDGFRTSHELMKIERVTEDDILAMLSEEAIDEHRARRLTPDRPVIRGT
                     AQNPDVFFQAREAAGPFHLAVPEIVDGVMDRFHELTGRRYRPFEYVGHPEAERVLVLM
                     GSGVGAAEEAVERLVAEGERVGLVKVRLFRPFSIERFAEALPDAVRSIAMLDRTKEPG
                     SVGEPLYQDVVTALVERRGRPVPVVIGGRYGLSSKEFTPAM"
BASE COUNT          513 a         1223 c         1279 g          557 t
ORIGIN      
        1 gtgtaggtcc cgtcctgggg cagcacgacc tcgtgatcca cgtagatcga gtcgaacagc
       61 tccgagccgt cgggcgcgtg gaggctccag gcgatctggg tgttgtcccc cgtgagcgcg
      121 tcgaggatgg ccacctgtcc catcgtcccc tcgaacaggt agcggtcaat cgcccccggc
      181 ccctccagat tgccggcccc cggcccgggc acgccgtcgg agaccgtgtc cccgaaggcg
      241 atcgtgaact cctgcgttcc cgccccctgc agcagctgga aggagtacgt cccggtgctg
      301 gcgatgttca ccccgctcat cgtcagcacg tacgtgccgg tctgggcgag cgtctcctgg
      361 tgatcgatgt agagccggtc gaacacctgc gtgccgtcgg gcgcgtgcag ggcccagcgc
      421 acctgcgtgt tgttcccggt cagcgcgtcg aagatggcca cctgccccgc cgtggcgtcg
      481 aagaggtagc ggtcgatggc ccccgggacc tcgaggttcc cggctccggg cgccgggacg
      541 tcctgggaga ccgtgtcccc gaaggcgatc gcgaactcct ggggaggcgg ggtcaggagg
      601 agccggaacg agtaggtccc ggtcgaggcg gcggagaacc cccggaccgt cagcacgtag
      661 gtgccgttct gtggcagctc gacctcccga tccacgtaga ggccgtcgaa gagggcgcct
      721 cccccgggcc cctccaggcg ccagaggaac gtggtgttgc tccccgccag cacgtcgaag
      781 atcgcgacgt cccccgccgc cccctcgaag ctgtaggcgt cggtgccccc cgcgacctcg
      841 atgttgccgg cgccgggtcc cgggaccccg tcagcgacgg tgtctccgta ggcgatctcg
      901 aaggagtcgg cgacctgcgc tcccgccggc gtccccggaa ggacgcccac gagaagggcg
      961 atcaggacga gggttcggat gaagacgcgg ctcatgacga cctccgatcg tgagcaggta
     1021 caggacggct gtgtggcggg gcacgctcgc tcccttgacg gacatacgta ccgtacgtat
     1081 gtgggaattt acccgtttgc ctgcggtttc gtcaacccct gaggtaacat tcgtacgtac
     1141 cgtatgcatc gcggaggagg ggcatggcaa ccgaccgatc gcgcaggcgc cgctcgaggc
     1201 ttcccaggga gcacgctcgg aagaggtaca tcgagatcgg cgagctcgtc gtcctcgagc
     1261 agatcaagga ggattcggag gcgctggaca ggcggtccac tgccgtgggg ccgttcgccc
     1321 gtctcgacgc gaacgctgtc gcggcccgcg agggcaagac ccggggggcc gtcaacaacg
     1381 tcttcgggag tcaggccgcc tttcaggcgg agacgatggc ggacaccctg ttcgccggcg
     1441 actggatccg tcagatccga tatccgcgtc cgcgcgactt cccggacgcc gaggcctggc
     1501 tggacaggct gctgaccggt gagtcggggc gtggccctct ccacggggcc gagccgacca
     1561 tgacctacgg gttcctctgg gccctgtggc tgagcgccgt gccgtacggc ctatggagcg
     1621 atgagatctc ccgtcccagc ttggaggagt tcgtgaagcg ggtcgaaagc ctggagcgcg
     1681 tgatcgagga ggctctggac cacttcgagg tctccctccg cgcggggacc accatgaccg
     1741 acctggccac ggcgctcgcg agcctgattg agggcgtgtg gctcaaccag tgcctcacgg
     1801 agcgtcaccc gtccgatgcg gatgcgccta tctcggagat gctgcgacgg tccggacggc
     1861 tcctgtggcg cggcgccgtc tcctcctaga ccgagcccgc ggaactcggc gcgtcgatgg
     1921 aggctgtgtc cgcaaccttc gccgacgaag gtcccggatg catggggccc tccggccctg
     1981 cgacgtgatc ggccggtcct gcatgctcgc tcggagacgc gtcgaggagg tcacggatga
     2041 gcgagcagag cccgttcccc aggtcgatct acggaggagc gttccgggag gggttcgtcg
     2101 ccgcgctggt ggacagccgg gtcgaggcga cgcgcctggt ggatctgctg tcggtcaccg
     2161 gcatccggcg gagcgacgtg ggggtccttc ccggggacct ggcgctcgtg atcgacgggt
     2221 cgcaccatcc gtcgctggcc gtctccgagg ccgctccggg cgaggcggcg gtctccgaga
     2281 ggtacgtgtc cggcgccctg ctgggggacg tgctcgtcgc cgttcgcacc ccgtccgagc
     2341 cacacgctcg ggccgtggcg cggatcctcc tctcggaggg ggcttcgctg gtctaccact
     2401 tcggacccgg tttcctccga gctcccgata tgaccgaggc tgcggcgtga cccgtccggt
     2461 cgtcaccgtc gacggcaacg aggccgccgc atacgtcgcc cacaaggcca gcgaggtcat
     2521 cgcgatctac ccgatcaccc ccgcctcccc catgggagag ctggccgacg cctggtcggc
     2581 ccggggggag cccaacgtct gggggagcgt cccggaggtc atcgagatgc agagcgaggg
     2641 cggggcggcc ggcgccgtgc acggagccct gcaggcgggg gccctcacga ccaccttcac
     2701 ggcatcgcag gggctcctgc tgatgctgcc gaacatgtac aagatcgcgg gcgagctcac
     2761 cccggcggtc ttccacatcg ccgcccgcac cgtcgccacg cacgccctct cgatcttcgg
     2821 ggaccacagc gacgtgatgg gggctcgctc cacgggatgg gccatgctgg cgtcgtcctc
     2881 ggtccaggag gcccaggacc tggcgctggt ggcgcacgcc gcgaccctcg agtcgcgggt
     2941 gcccttcctc cacttcttcg acgggttccg gacgtcgcac gagctgatga agatcgagcg
     3001 cgtcaccgaa gacgacatcc tggccatgct gagcgaggag gcgatcgacg agcaccgcgc
     3061 ccggcgcctc acgcccgacc gccccgtgat caggggcacg gcccagaacc cggacgtgtt
     3121 cttccaggcg agggaggcgg ccggcccctt ccacctcgcc gtcccggaga tcgtggacgg
     3181 cgtgatggat cgcttccacg agctgaccgg gcgccgctac cggccgttcg agtacgtggg
     3241 gcaccccgag gccgagcggg tgctggtcct gatggggtcc ggggttggcg cggccgagga
     3301 ggccgtggag cgcctggtcg cggagggcga gcgggtgggc ctggtcaagg tgcggctgtt
     3361 ccgcccgttc tcgatcgagc ggttcgccga ggccctgccg gatgcagtcc gctcgatcgc
     3421 catgctcgac cggacgaagg agcccgggtc ggtgggcgag cccctgtacc aggacgtggt
     3481 caccgcgctc gtcgagcgcc gcggccgccc ggtgccggtc gtgatcgggg gacggtacgg
     3541 cctgtcctcc aaggagttca cgccggccat gg
//
LOCUS       DASUTW010000017        10727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1173385, whole genome shotgun sequence.
ACCESSION   DASUTW010000017 DASUTW010000000
VERSION     DASUTW010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10727
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173385"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VF058_00585"
     CDS             complement(<1..867)
                     /locus_tag="VF058_00585"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX6206832.1"
                     /translation="MKPRPLPPGGTIGVPAPASPWQDRSEWDRGIRWWESKGYTVKLA
                     KNAMARDDYVAGDPADRARDVEQMFADPEVDAVQAFGGGYGSSQVVPHLDTDVIAQNP
                     KPFVGYSDITALHVAIRQATGLVTFYGPGLAGIAYEASDDREFTQGRALRALTDPTPL
                     GEIPARPDDAYVGAIGAGVARAPLVGGNLWLVRETLGTPWEIETAGCVLFFEDVYTPP
                     WHVDGMITHLANVGKLREVVGVVVGEMYRSEEWRHPEPWLRSRSMEDVLEYHLGPLGV
                     PVLTNLPLGHGKH"
     gene            complement(879..1676)
                     /locus_tag="VF058_00590"
     CDS             complement(879..1676)
                     /locus_tag="VF058_00590"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX6206833.1"
                     /translation="MRTTPLRYERLTWPEVAEAAREDAVCLIPVATLEDHGPHLPIDA
                     DLRIVAEIAERAASAAGTILLPAIPHGYSPHHLDFPGPITIGWDTFTRYVRDVGISLA
                     DHGFRRMLFLNGHGSNQNLVETAARLVMVERPDTLAAAAFYLTSEKGLAEVERIRESE
                     RGGMAHACELETSIYLAIEPEAVAMDLAVDEMSYPAGDHAWIDWTDGPLKLMPWWSSF
                     SETGVQGRPTLATAEKGEALLEAAVAEVSAYLEELREKPLPERREPT"
     gene            complement(1676..2704)
                     /locus_tag="VF058_00595"
     CDS             complement(1676..2704)
                     /locus_tag="VF058_00595"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEX6206834.1"
                     /translation="MIPVVRVSGGAEERGRAVGEALAEPIGRSLDFYRGYLQRRGISR
                     ESLPLILRPHRRAAEGALPDLVAEIDAMAEGAGADPWDLFAVNAFEELEPAMSEPSME
                     RCTSFTASTPGGPILAHNEQWLAGDLGNVAVVVADPTDGPGFASPTVVACLPAVGINN
                     AGLAQAIMSLSAGDDREGVPRVLVSRHALQARFSDDAVKRADLPGRAGGYAHMLAGGG
                     GAAFTIETTAERLAVLDGHGPHTNHYLHQDLTLIGDRPRPGSVSRLERARELLEDRPP
                     RSPEDAMAVLADHDGHPQPICLHPDPEDGDDAEAVVFSMVCSLRDRRMWVAPGPPCST
                     PFEEIEVA"
     gene            complement(2701..4080)
                     /locus_tag="VF058_00600"
     CDS             complement(2701..4080)
                     /locus_tag="VF058_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6206835.1"
                     /translation="MGGDQAAAIVGQRFDAYLERIRDLCRMPSVSATGEGVREVADAV
                     LGIIQDAGGEAELVETPGFPVILGEIPGPPGSPSLLRYGMYDVQPAEEPDWTVPPFSA
                     EIVDLDGIGPSVVCRGVANSKGSLASFFCALDALRDAGGIPCTIRFVIEGEEELGSMN
                     LPQVVRERAEDLRSDCGADLDLCGERDGSVQLITGCKGLLSLELVCRAGGWGGPAKPL
                     HSSEAAWIASPVWSLVHALATIVGPGEELLVREITGAAAPPSDDDRPLLRELAAGFDP
                     DEHLRDARASRYKIEGSPEELLEALIYRPTANIDGLEAGYTGPGHTIMPSEARAVLDL
                     RLVPDLDPDEAATAIRRHLDREGFPHVQVVPMERYPWAKSAPDSRASRAMRASYEELG
                     RRPLPYPMAPWCAPFYIFDRLLEIPWASGGLGFSGGAHAPDEFATVDGLREHILGTVG
                     FLLAYAEAA"
     gene            complement(4331..5209)
                     /locus_tag="VF058_00605"
     CDS             complement(4331..5209)
                     /locus_tag="VF058_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEX6206836.1"
                     /translation="MSEPTLSPELRGEERPLVALETSVLAQGLPEPRNREAADRMEEA
                     VRAAGALPAWTWVDEGAVRVGATGDELDRLCAEAAAKVARRDLPMAVAGRGLGATTVS
                     ATLWIAARAGIEVAATGGIGGVHRGGGDVSADLAELARSPVTLVCAGPKSITDPEATL
                     ERLEELGVAVVGYGTERLPFFLVRETPIALEHRADSPPEAAEVARARRDLGLAAALLM
                     CVPIPEASALDADEVASAVARCEERAAREAVTGKNVTPFLLRCLAAETGGRSLEANMA
                     LLEENARVAGAIAAAL"
     gene            complement(5206..6063)
                     /locus_tag="VF058_00610"
     CDS             complement(5206..6063)
                     /locus_tag="VF058_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX6206837.1"
                     /translation="MTDLVVVGDALLDVAARAAALLEGGDVRGSVRARAGGQGANVAA
                     WAGSEGLRVRLHARIGDDAAGRLVRESLEERGVEAALTVDPEAPTGTLLVLVRGGDRS
                     MVADRGAAARLSPSDLPDEIEAGAVLVSGYLLFDQGSEAAGLAALERARARHLAVEAS
                     TWPLLEAYGSERFLEATSAATVLLANEREAEVLTGAEGEEAAAKLAERYEVAVVKLGD
                     RGAALASAELTLHEPAKQVEVADPTGAGDAFDAAFLAALLRGGSLGRALDHAITTGGW
                     VASREEAWP"
     gene            complement(6060..7307)
                     /locus_tag="VF058_00615"
     CDS             complement(6060..7307)
                     /locus_tag="VF058_00615"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6206838.1"
                     /translation="MARPKVLVLGGGYAGVMAAAGLGGRADVLIVSRDNFLLFTPMLA
                     EVAAGDLDPRHVVSPIRQLAPHARLLIGDIEEIDLGSKTVAVRPKLRRGVVRYRADAI
                     VLGLGAVQATFGIEGVDEHALPFKTISDALRIRNRMLASLEAAAESHDPRLTKVAVVG
                     AGYSGAELATALADMLSEAAARFYRTAPRPHVTLVDALERVTAMLPERLSAEAERGIR
                     DRGIEPVLGAKVAKVHEGGISLEDGREIEAGTVIWAAGVRPNPLVETLGLPTGPGGRL
                     VVDEHLQAVPGVFAGGDLAAVPDGRGGISPPTAQFALRQGVYLGRHLSDVLEGRSAPP
                     FRYRTLGELVSLGHRNAVGLVFGVPVTGVPAWFLWRSYYLMRLPTLLRKARVALDWTL
                     DVVFPPDIAWLPTSDLGPDPSEP"
     gene            7365..7991
                     /locus_tag="VF058_00620"
     CDS             7365..7991
                     /locus_tag="VF058_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HEX6206839.1"
                     /translation="MARILLIRHAVADHTGKRLYGRSRGVALSDRGRADAEALAERLR
                     PVRIHGLYVSPMQRCRETAAAIARGRRLEPEIVEDLNEVDYGDWTGRTFASLRRTKVW
                     RQVRTRPSTVRFPNGESLLEVQERAVRAIEGIARRHPRGVAAVVTHGDVIRLALAHYA
                     GLHLDLFQRLEASTASVTALALGDGLPRVLRANDTGRLEDLVPGKVGG"
     gene            7996..8502
                     /locus_tag="VF058_00625"
     CDS             7996..8502
                     /locus_tag="VF058_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HEX6206840.1"
                     /translation="MELDPVDRITVGAVGEPGERTFYLQARRGTDLVTIVVEKQQVQL
                     LSATVVDMLSRLGTDVDAEVGEDEMELEQPIVPEWRASRLAVGYEEERDLFMLEAEEL
                     EEEDEETELGGQARFWATREQMLALARHGIVVAAAGRPTCEFCGNPMDPEGHVCPAMN
                     GHGRLGEA"
     gene            8499..9182
                     /locus_tag="VF058_00630"
     CDS             8499..9182
                     /locus_tag="VF058_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HEX6206841.1"
                     /translation="MTTALAGGDLEVLGLLPGASNHTFLGRIGGIHVVYKPIRGEAPL
                     WDFPIGTLAAREVAAARLAQALGWPNVPSTVMRDGPAGPGAVQEYVLAVEGEHYFTLR
                     GERADELRAVAAFDVVANNADRKGGHLLLGEDGRLWCIDHGLTFHAEPKLRTVIWDFA
                     GEPLPGGLAEDLERVVGELRGGPLREGLEGLLDPTEVGAAGARAEALLRAGRFPGPGP
                     GRPVPWPLV"
     gene            9182..9997
                     /locus_tag="VF058_00635"
     CDS             9182..9997
                     /locus_tag="VF058_00635"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6206842.1"
                     /translation="MGALERWREALASWAIPEEVLAKAPESPWGFRPEIFADRARRAP
                     DEPETPSRRRAGAALRGGGTVLDVGAGAGAASLPLAPPADAIVAVDPSPELLAAFEEL
                     AAERGLTLTSIEGSWPEAAGETPACDVVVCHHVLYNVPDLAPFARALTAHARRLVVVE
                     LTAEHPLTWMAGLWRRFHGLERPIEPTADVAEAALREAGIDPGREDHEVRREAGGFPD
                     RDAAVAQARRMLCLPPDRDPEVAEALGDRLREGSTGWGIGPPAARVVTLWWEP"
     gene            complement(9975..10394)
                     /locus_tag="VF058_00640"
     CDS             complement(9975..10394)
                     /locus_tag="VF058_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HEX6206843.1"
                     /translation="MSTPLARKLGIKAGSSVVFVGQPDGFEGMLEPLPRGVSIATRVE
                     GPHDVVVLFAHRRSALERRLPSLRKALDYAGGLWIAYPKKASGVPSDLDFAVVQETGL
                     AAGLVDNKSSSIDETWTAVRFVYRLSDRPEVTAPTTG"
     gene            complement(10391..>10727)
                     /locus_tag="VF058_00645"
     CDS             complement(10391..>10727)
                     /locus_tag="VF058_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206844.1"
                     /translation="FHLAGRLLARHPRATRQVIMVTDGEPTAHLEGDEVFFSWPPVRE
                     TIRRTLSEAMRLARAGVTLNVFMLEDSPGLARFMERLARLTSGRIFLMSGETMGDFVL
                     RDYVAGRAS"
BASE COUNT         1394 a         3897 c         4078 g         1358 t
ORIGIN      
        1 gtgcttcccg tgccccaggg gcaggttggt cagcaccggg accccgaggg gcccgaggtg
       61 gtactccagc acgtcctcca tcgagcggct ccggagccag ggctccggat gccgccactc
      121 ctcgctgcgg tacatctcgc cgacgaccac gcccaccacc tcgcggagct tccccacgtt
      181 ggcgaggtgg gtgatcatgc cgtccacgtg ccacggcggc gtgtagacgt cctcgaagaa
      241 gaggacgcag ccggccgtct cgatctccca aggggtgccc agcgtctctc gcaccagcca
      301 gaggttgccg ccgacgagcg gggcgcgggc gacgccggcg ccaatcgcgc cgacgtaggc
      361 gtcgtcgggg cgggccggga tctctcccag cggggtgggg tcggtgaggg ctcggagcgc
      421 tcgaccctgg gtgaactccc ggtcgtccga ggcctcgtag gcgatgccgg ccaggccggg
      481 accgtagaag gtgaccagcc cggtggcctg gcggatcgcg acgtgcaggg cggtgatgtc
      541 cgagtacccg acgaagggct tggggttctg ggcgatcacg tccgtgtcga ggtgggggac
      601 gacctgcgag gagccgtagc cgccgccgaa ggcctggacg gcgtcgacct cggggtccgc
      661 gaacatctgc tccacgtcgc gggcccggtc ggccgggtcg cccgccacgt agtcgtcgcg
      721 ggccatcgcg ttcttggcga gcttcaccgt gtagcccttc gactcccacc agcggatccc
      781 gcgatcccat tccgagcggt cctgccaggg cgaggcgggg gcggggacgc cgatcgtgcc
      841 gccgggcggg agggggcggg gcttcaccgg ggggcgggtc acgtgggctc ccggcgctcg
      901 gggaggggct tctcgcggag ctcctccagg taagcggaga cctcggccac ggccgcctcc
      961 agcagggcct cgcccttctc cgcggtggcc agcgtgggtc ggccctggac gccggtctcg
     1021 gagaacgagg accaccaggg catcagcttc agcggcccgt cggtccagtc gatccaggcg
     1081 tggtcgcccg cgggatagga catctcgtcc acggccaggt ccatggccac ggcctcgggc
     1141 tcgatggcga ggtagatcga ggtctccagc tcgcaggcgt gggccatgcc gccgcgctcg
     1201 gactcgcgga tccgctccac ctcggccagc cccttctcgg aggtgaggta gaaggcggcg
     1261 gcggcgaggg tgtccggtcg ctccaccatg accaggcgcg cggccgtctc cacgaggttc
     1321 tggttggagc cgtggccgtt gaggaagagc atccggcgga agccgtggtc ggccagcgag
     1381 atccccacgt cgcggacgta gcgggtgaag gtgtcccagc cgatcgtgat cggccccggg
     1441 aagtcgagat ggtggggcga gtagccgtgg gggatcgccg ggaggaggat cgtgccggcg
     1501 gccgaggccg cgcgctcggc gatctcggcc acgatccgca ggtccgcgtc gatcggcaga
     1561 tggggtccgt ggtcctcgag ggtggcaacc gggattaggc agacggcgtc ctcgcgcgcg
     1621 gcctcggcca cctcgggcca ggtgagccgc tcgtagcgga ggggcgtcgt ccgcatcagg
     1681 ccacctcgat ctcctcgaag ggggtggagc agggcgggcc cggggccacc cacatcctcc
     1741 ggtcgcggag gctgcagacc atggagaaga ccaccgcctc ggcgtcgtcg ccgtcctcgg
     1801 ggtcggggtg gaggcagatc ggctgggggt gaccatcgtg gtcggcgagg accgccatgg
     1861 catcctcggg cgaccgcggg gggcggtcct cgaggagctc gcgggcccgc tccagccgcg
     1921 agacgctgcc gggccgcggc cggtccccga tcagcgtgag gtcctggtgg aggtagtggt
     1981 tcgtgtgggg gccgtgcccg tcgaggacgg ccaggcgctc agccgtggtc tcgatcgtga
     2041 aggccgcgcc gccgccgccg gcgagcatgt gcgcgtagcc gccggcccgc ccgggcaggt
     2101 ccgcccgttt gaccgcatcg tcgctgaagc gcgcctggag ggcgtgccgc gagacgagga
     2161 ctcgcggcac gccctcccgg tcgtcccccg cggagagcga catgatcgcc tgggccaggc
     2221 ccgcgttgtt gatgccgacc gccgggagac aggccacgac ggtgggcgag gcgaagccgg
     2281 ggccgtcggt cgggtccgcc acgaccaccg ccacgttccc caggtccccg gcgagccact
     2341 gctcgttgtg ggcgaggatc gggccgcccg gggtggacgc cgtgaacgag gtgcagcgct
     2401 ccatcgaggg ctcggacatg gccggctcca gctcctcgaa ggcgttgacg gcgaagagat
     2461 cccagggatc ggcgccggct ccctcggcca tggcgtcgat ctcggctacg aggtcgggga
     2521 gggccccctc ggcggcccgg cggtgtgggc ggaggatcag ggggagggac tcgcgggaga
     2581 tgccgcggcg ctggaggtac ccgcggtaga agtcgagcga gcggccgatc ggctcggcca
     2641 gcgcctcccc cacggcgcgg ccgcgctcct cggccccacc ggatactcgg accaccggga
     2701 tcatgcggcc tccgcgtagg ccaggaggaa ccccaccgtc cccaggatgt gctcgcggag
     2761 gccgtccacg gtggcgaact cgtccggggc gtgcgcgccg ccgctgaacc cgaggccgcc
     2821 cgaggcccac gggatctcca ggaggcgatc gaagatgtag aagggagcgc accagggggc
     2881 catcgggtag gggagcggcc ggcggcccag ctcctcgtag gaggcgcgca tggcgcggga
     2941 ggcgcgggag tcgggcgcgg acttggccca ggggtaccgc tccatcggga cgacctggac
     3001 gtgggggaac ccctcgcggt ccaggtggcg tcggatcgcg gtcgcggcct cgtcggggtc
     3061 gaggtcgggg accaggcgga ggtccagcac ggcccgggcc tcgctgggca tgatcgtgtg
     3121 gccgggcccc gtgtacccgg cctcgagtcc gtcgatgttg gcggtggggc ggtagatcag
     3181 ggcctcgagg agctcctcgg gggagccctc gatcttgtag cgggaggctc tcgcgtcgcg
     3241 gaggtgctcg tccgggtcga agccggcggc cagctcgcgg aggagggggc ggtcgtcgtc
     3301 cgaggggggc gcggcggcgc ccgtgatctc gcggacgagg agctcttcgc ccgggccgac
     3361 gatcgtggcc agcgcgtgga ccagcgacca cacgggcgac gcgatccagg cggcctcgct
     3421 ggagtggagg ggcttggcgg gcccgcccca gcccccggcc cggcagacga gctccaggct
     3481 gaggagcccc ttgcagcccg tgatgagctg gaccgagccg tcgcgctcgc cgcagaggtc
     3541 gagatcggcg ccgcagtccg agcggaggtc ctcggcccgc tctctgacga cctgcgggag
     3601 gttcatgctg cccagctcct cctccccctc gatcacgaag cggatcgtgc aggggatccc
     3661 ccccgcgtcg cggagggcgt cgagcgcgca gaagaacgag gcgaggctgc ccttggagtt
     3721 cgccacgccg cggcagacga cggaggggcc gatgccgtcc aggtccacga tctcggccga
     3781 gaacgggggg acggtccagt cgggctcctc ggccggctgg acgtcgtaca tgccgtagcg
     3841 gaggagcgag ggggagcccg gcggccccgg gatctcgccc aggatcacgg ggaagcccgg
     3901 ggtctcgacg agctcggcct cgccccccgc gtcctggata atcccgagga cggcgtcggc
     3961 gacctcgcgg acgccctcgc cggtggcgct gaccgagggc atgcggcaga ggtcgcggat
     4021 gcgctccagg taggcgtcga agcgctggcc gacgatcgcc gcggcctgat caccccccac
     4081 cgcctgctgc gtaccgacct ccttgtcgtt ggctgattat gaacggctcg cgcccggagg
     4141 gaacgacgga acggcgcaat ggtcgaaggc ggaccctccc gaacggcgac ctcccgatcg
     4201 agcagcggtt ctcccgctct cgacccccaa agcgtcgaga gcgggagaac ctggtcccgg
     4261 aacgctcgcg cccccccgtt cgcgtcccgc caccagatac gctcgttccg gttcctcgga
     4321 ttggagaagt tcagagggcg gcggcgatcg cgccggccac gcgggcgttc tcctcgagga
     4381 gggccatgtt ggcctcgagg ctgcggccgc ccgtctccgc ggccaggcac cggaggagga
     4441 acggggtgac gttcttgccc gtgacggcct cccgtgcggc gcgctcctcg catcgtgcga
     4501 cggcgctcgc cacctcgtcg gcgtcgagcg cggacgcctc ggggatcggc acgcacatga
     4561 ggagggcggc ggcgaggccc agatcccgcc gggcccgggc gacctccgcc gcctccgggg
     4621 gcgagtcggc tcggtgctcc agggcgatcg gggtctcgcg gaccaggaag aaggggaggc
     4681 gctcggtccc gtagcccacc acggcgaccc ccagctcctc gaggcgctcc agggtcgcct
     4741 ccggatcggt gatcgacttg gggccggcgc agaccagggt gacgggggag cgggccagct
     4801 ccgcgaggtc ggccgagacg tcgccgcctc cacggtggac gccgccgatc ccaccggtgg
     4861 cggcgacctc gatcccggcg cgggcggcga tccagagggt ggccgagacg gtggttgcgc
     4921 cgaggccgcg gcccgccacg gccatcggga ggtcgcgccg ggcgaccttg gccgcggcct
     4981 cggcgcacag ccgatcgagc tcgtcgccgg tcgccccgac ccgcacggcg ccctcgtcca
     5041 cccacgtcca cgcggggagg gcgcccgccg cgcgcacggc ctcctccatc cggtcggcgg
     5101 cctcgcggtt cctcggctcg ggcaggccct gggccagcac gctcgtctcc agagccacga
     5161 gggggcgctc ctcgccgcgg agctcggggg agagcgtcgg ctcgctcacg gccaggcctc
     5221 ctcgcgcgag gccacccatc cgcccgtggt gatggcgtgg tccagcgcgc gacccagcga
     5281 tcccccgcgg aggagcgccg ccaggaaggc ggcgtcgaac gcgtccccgg cgccggtggg
     5341 gtccgcgacc tcgacctgct tggcgggttc gtgaagcgtc agctcggccg aggccagcgc
     5401 ggcgccgcga tccccgagct tcaccaccgc cacctcgtac cgctccgcga gcttcgccgc
     5461 cgcctcctcg ccctcggcgc ccgtcagcac ctcggcctct cgctcgttgg ccagcagcac
     5521 ggtggcggcc gacgtcgcct cgaggaaccg ctccgagccg taggcctcga ggaggggcca
     5581 tgtcgaggcc tcgaccgcga ggtgccgggc ccgcgcccgc tccagcgcgg ccagaccggc
     5641 cgcctccgag ccctggtcga agagcaggta gccggagacc agtaccgcgc cggcctcgat
     5701 ctcgtccggg agatccgacg gcgagagccg cgccgccgcg cctcggtcgg ccaccatcga
     5761 tcggtcccct ccgcgcacga gcaccagcag ggtcccggtc ggcgcctccg ggtcgacggt
     5821 cagcgccgcc tccacgcccc gctcctcgag cgactcgcgg accaggcggc ccgccgcgtc
     5881 gtccccgatc cgcgcgtgga gccgcacgcg cagcccctcg ctccccgccc acgcggcgac
     5941 gttcgccccc tggccgcctg ctcgcgctcg caccgagccc cggacgtcgc ccccttcgag
     6001 cagtgccgcc gcgcgggccg cgacgtcgag cagggcgtcc cccaccacga cgaggtcggt
     6061 cacggctccg aggggtcggg gcccaggtcg ctcgtgggga gccaggcgat gtcgggaggg
     6121 aacacgacgt ccagcgtcca gtccagcgcc acgcgggcct tgcgcagcag cgtggggagg
     6181 cgcatcaggt agtagctcct ccagaggaac caggccggca cgccggtcac cgggaccccg
     6241 aagaccaggc ccacggcgtt gcggtggccc agcgagacga gctcgcccag ggtccggtag
     6301 cggaacgggg gggcggaccg gccctccagc acgtccgaga ggtggcgtcc gaggtagacg
     6361 ccctgccgca gggcgaactg ggcggttggg ggcgagatcc ctccgcgacc gtcggggacc
     6421 gcggccaggt cgccgccggc gaacacgccg ggcacggcct gcaggtgctc gtccacgacc
     6481 agcctgccgc cgggccccgt ggggagcccg agcgtctcca cgagggggtt cggccggacg
     6541 ccggcggccc agatcacggt gccggcctcg atctcccggc cgtcttcgag cgagatgccg
     6601 ccctcgtgca cctttgccac cttcgccccg aggacgggct cgatgccgcg gtcgcggatc
     6661 ccgcgctcgg cctcggcgct cagccgctcg ggaagcatgg cggtcacgcg ctccagggcg
     6721 tccacgaggg tgacgtgagg ccgcggcgcg gttcgataga accgcgccgc ggcctccgag
     6781 agcatgtccg cgagcgcggt ggctagctcc gcgcccgagt acccggcgcc cacgaccgcc
     6841 accttcgtca ggcgcgggtc gtgcgactcg gccgccgcct ccagcgaggc cagcatccgg
     6901 ttccggatcc gcagggcgtc ggagatcgtc ttgaacggca gggcgtgctc gtcgaccccc
     6961 tcgatcccga aggtggcctg caccgccccc aaccccagca cgatcgcgtc ggcacggtac
     7021 cggacgaccc cccggcgcag cttcggccgg accgcgaccg tcttggaccc gaggtcgatc
     7081 tcctcgatgt cgccgatcag cagccgggcg tggggggcga gctggcggat cgggctcacg
     7141 acgtgccgag ggtcgagatc cccggccgcc acctcggcca gcatgggcgt gaagagaagg
     7201 aagttgtcgc ggctgacgat gaggacgtcg gcccgccccc ccaggccggc cgcggccatc
     7261 acccccgcgt accctccccc cagcaccagc accttcggcc tcgccatgcc tcgcatcgta
     7321 gtgcccggcg gaccgacccg accggccgcc ttatgctggg ggtcgtggca cgcatcctcc
     7381 tgatccggca cgcggtggct gaccacacgg gaaagcgcct ctacgggcgt tcccggggcg
     7441 tcgcactctc cgatcggggc cgggccgacg ccgaggcgct ggccgagcgc ctccgcccgg
     7501 tgcggatcca cggcctctac gtcagcccga tgcagcggtg ccgagagacg gccgctgcga
     7561 tcgcccgcgg ccgccggctg gagccggaga tcgtcgagga cctcaacgag gtcgactacg
     7621 gcgactggac ggggaggacg ttcgcctcgc tccgccggac gaaggtctgg cggcaggtcc
     7681 ggacgaggcc ctcgaccgtg cggttcccga atggggagag cctcctcgag gtccaggagc
     7741 gggccgtccg ggcgatcgag gggatcgccc gtcgccaccc gaggggcgtg gcggccgtcg
     7801 tgacgcacgg cgacgtgatc cgcctcgcgc tcgcgcacta cgccggcctc cacctcgacc
     7861 tgttccagcg gctcgaggcg tccaccgcct cggtcaccgc gctcgcgctc ggggacggcc
     7921 tcccgcgcgt cctccgcgcc aacgataccg ggcgtctcga ggacctcgtc ccggggaagg
     7981 tgggaggata ggcgcgtgga gcttgacccc gtggaccgca tcaccgtcgg agcggtcggg
     8041 gagcccggcg agcgcacctt ctacctgcag gcccgccgcg ggaccgacct cgtgacgatc
     8101 gtcgtggaga agcagcaggt gcagctgctg tcggcgacgg tggtggacat gctctcccgg
     8161 ctcgggaccg acgtggacgc cgaggtgggc gaggacgaga tggagctcga gcagcccatc
     8221 gtccccgagt ggcgggcctc gcgcctcgcg gtgggctacg aggaggagcg ggacctcttc
     8281 atgctcgagg ccgaggagct ggaggaggag gacgaggaga cggagctggg cgggcaggcc
     8341 cgcttctggg ccacccgcga gcagatgctg gcgctggccc gccacgggat cgtcgtggct
     8401 gcggccggga ggcctacctg cgagttctgc ggcaacccga tggaccccga gggccacgtc
     8461 tgcccggcga tgaacggcca cggacggctg ggggaggcgt gaccaccgcc ctggcaggcg
     8521 gcgacctcga ggtgctgggg ctcctgcccg gcgcctcgaa ccacaccttc ctgggccgga
     8581 tcggagggat ccacgtggtc tacaagccga tccgcgggga ggcgccgctg tgggacttcc
     8641 cgatcgggac gctggcggcg cgcgaggtgg ccgcggcgcg gctggcccag gccctgggat
     8701 ggccgaacgt gccgtcgacc gtcatgcggg acggaccggc cggccccggc gccgtccagg
     8761 agtacgtgct ggcggtggag ggcgagcact acttcacgct gcgcggggag cgggccgacg
     8821 agctccgagc ggtggcggcg ttcgacgtgg tggcgaacaa cgcggatcgg aagggcggcc
     8881 acctcctcct gggcgaggac gggcggctct ggtgcatcga ccacgggctg acgttccacg
     8941 ccgagccgaa gctgcggacg gtgatctggg acttcgccgg cgagcccctc cccggggggc
     9001 tggcggagga cctggagcgg gtcgtgggcg agcttcgggg cgggccgctg agggaggggc
     9061 tggagggcct gctcgacccg accgaggtgg gggcggccgg ggcccgggcc gaggcgctgc
     9121 ttcgggccgg ccggttcccc ggcccgggcc ccggccgccc cgtcccctgg ccgctggtct
     9181 gatgggcgcg ttggagcgct ggcgcgaggc gctggcctcc tgggccatcc ccgaggaggt
     9241 cctggcgaag gccccagagt cgccctgggg gttccgcccc gagatcttcg ccgaccgggc
     9301 ccggcgggcg ccggacgagc ccgagacgcc gtcgcgacgc cgcgccggcg ccgccctgcg
     9361 gggcggcggg accgtcctcg acgtgggggc cggcgccggc gcggcgtcgc tccccctcgc
     9421 tccgcccgcc gacgcgatcg tggccgtgga cccctccccc gagctgctgg cggccttcga
     9481 ggagctggcc gccgagcggg gcctgacgct gacgtcgatc gaggggtcgt ggcccgaggc
     9541 ggccggcgag acgcccgcct gcgacgtcgt cgtctgccac cacgtcctct acaacgtccc
     9601 cgacctcgcc ccgttcgccc gcgcgctcac ggcccacgcc cgtcggctgg tcgtcgtgga
     9661 gctcaccgcg gagcatccgc tcacctggat ggccggcctc tggcgccgct tccacgggct
     9721 ggagcgcccc atcgagccca ccgccgacgt ggccgaggcc gccctgcgcg aggccgggat
     9781 cgatcccggg cgcgaggacc acgaggtccg gcgcgaggcc ggcggcttcc ccgatcgaga
     9841 cgccgccgta gcccaggccc gccggatgct gtgcctgccc cccgatcgcg atcccgaggt
     9901 ggccgaggcg ctgggcgacc gcctccgcga ggggtcgacg gggtggggga tcgggccgcc
     9961 cgcggcccgc gtcgtcaccc tgtggtggga gccgtgacct ccgggcgatc cgagagccgg
    10021 tagacgaacc ggaccgccgt ccaggtctcg tcgatcgagg acgacttgtt gtccacgagc
    10081 cccgcggcca ggcccgtctc ctgcaccacg gcgaagtcga ggtcgctggg aacgcccgag
    10141 gccttcttcg ggtaggcgat ccacaggccg ccggcgtagt ccagcgcctt ccggagcgag
    10201 gggagccgcc gctccagcgc cgagcgccgg tgcgcgaaca gcacgacgac gtcgtggggc
    10261 ccctcgaccc gggtagcgat gctcacccct cggggcaggg gctccagcat cccctcgaag
    10321 ccgtcgggct gcccgacgaa gacgacgctc gacccggcct tgatccccag cttcctggcc
    10381 agcggcgtgc tcacgaggcc cgccccgcca cgtagtcgcg cagcacgaag tcgcccatcg
    10441 tttcgcccga catcaggaag atgcggcccg aggttagcct cgccaggcgc tccatgaagc
    10501 gcgccaggcc cggggagtcc tcgagcatga acacgttgag cgtcacgccg gctcgggcca
    10561 gccgcatcgc ctccgacagc gtcctgcgga tcgtctcccg caccggcggc cacgagaaga
    10621 agacctcgtc gccctccagg tgcgcggtcg gctcgccgtc ggtgaccatg atcacctggc
    10681 gcgtggcccg cgggtgtcgg gccagcagcc gccccgccag atggaag
//
LOCUS       DASUTW010000018        14916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_266473, whole genome shotgun sequence.
ACCESSION   DASUTW010000018 DASUTW010000000
VERSION     DASUTW010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14916
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266473"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VF058_00650"
     CDS             <1..445
                     /locus_tag="VF058_00650"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX6206845.1"
                     /translation="RAPAPRLLGYHPGVAALQELLDSAPEDRRPTLRAVHKAIRAAAP
                     KLKMKIWRQKFWGGTDQTILGYGDITYRSSAGKDVEWFRIGLANQKSYVSVYVSATRD
                     RRYLPEIYGPRLGKVKVGRSSVSFRKLEDLDLDVLREMVAEADQA"
     gene            493..1224
                     /locus_tag="VF058_00655"
     CDS             493..1224
                     /locus_tag="VF058_00655"
                     /inference="COORDINATES: protein motif:HMM:NF027589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEX6206846.1"
                     /translation="MLQNDDMKEIAASGTLWGADGLAAVIGDVVGSREVEDRRRTQDE
                     LADALEGVNGRVPARSSLRPTIGDEFQGLYDSLGDALHITLLLRLLLKGRVEVRFGVG
                     WGHVEFFEGTTQDGPGWWGAREAIELVREAQDSRGEPRGLRTMFVARFAERSELEEKE
                     GLLPDLEGVINAALVTRDELVSAMDERDARIALGLMEGKTNAAIAEEEQVSTSAISQR
                     ALGAGIYAVLRADRLLRSAFEGAPA"
     gene            1221..1943
                     /locus_tag="VF058_00660"
     CDS             1221..1943
                     /locus_tag="VF058_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206847.1"
                     /translation="MSLLALWFAAVGLADLTTGLAGAAVGTARALSAAVVGAVAAAVG
                     ALLIGHEIGAVLFAGVVALALLVGWAWLRTGTWTNERVAGALALHATAILVLATLSGT
                     LPEGDGGVFARWLEGLPFVGASTWESGLLVVGIVLWLGATANSIVRMAIFLVEKQIDL
                     RVGEEQLKGGRIIGPLERWLIFSLVLSGNAVAAGFITAAKSLARFPELGRSANIRLDT
                     EYLLVGSLVSWTLALAPLLLVP"
     gene            complement(1947..2312)
                     /locus_tag="VF058_00665"
     CDS             complement(1947..2312)
                     /locus_tag="VF058_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX6206848.1"
                     /translation="MLITLVAGALVLGACGGDDGGDDGATGGGGSADVSVTGTDALAF
                     QPSELSAEAGSITVELTSEEGINHTFVVEEAGDEVVASADPGQTDTGTIELEAGSYTF
                     HCDVPGHRDAGMEGTLTVE"
     gene            2659..3126
                     /locus_tag="VF058_00670"
     CDS             2659..3126
                     /locus_tag="VF058_00670"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:2788..2790,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX6206849.1"
                     /translation="MLGEGARAPAFTLPDQDGNPISLSDYAGRWVVFWWFVRADTPGU
                     TMEGQGFRDRAPEFEELGAEILGVSYDPPEAQRAFAEKQGFAFRLLSDGDRTVAAAYG
                     TLRPDDHERAAFPRRFTYLIDPAGRVARAYVVKDVAAHPREVLEDLRALARGA"
     gene            3123..3911
                     /locus_tag="VF058_00675"
     CDS             3123..3911
                     /locus_tag="VF058_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015577189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HEX6206850.1"
                     /translation="MTRGSAGVLLDVDGVLVTSWEPLPGAVETIAWLRKSGVPFLLVT
                     NTTTRSRASLARDLVPFDVGPDDLLTAPMATAAYLRAEHPGARVHLMATGDVAEDLEG
                     IELVDGDADVVVIGGASEPDPEHPDTTFTYENLDRAFRMLLDGARLVAMHRNLSWRTS
                     RGVVLDSGAFVVGLERAAKVEAEVVGKPSAAFFRAALERLGMPADRALMVGDDVEADV
                     VGARSAGIRGVLVRTGKFRPEHLEAGSPDHVIDSIADLPGLLGG"
     gene            3998..4174
                     /locus_tag="VF058_00680"
     CDS             3998..4174
                     /locus_tag="VF058_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206851.1"
                     /translation="MPMNEDPLKRRLRDRDFARAYRSWVRLLVDRAKHRGSTRRVVRL
                     PRDGSLRPPKSLAG"
     gene            complement(4176..5294)
                     /locus_tag="VF058_00685"
     CDS             complement(4176..5294)
                     /locus_tag="VF058_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX6206852.1"
                     /translation="MFGNSWRVGRIRGIELRIDISWAIIAVLISYSLYWRYLEIYRGI
                     DNAYAILLGVGSAILFFGSVVFHEFAHAFTAQSRGIPVKSITLFLFGGATHAKVEAKG
                     PGAEFLIALVGPLSSLVLAGIFALLGTLGDAFLPREVAGAFGYLAFVNLALAVFNLVP
                     GFPLDGGRVLRAIIWGATGSLSKATRIASIAGQGFGYLLVAAGLFFVFNGALGAGIWF
                     GAIGWFLAQAARMSYEQLQLRRMLEDVDAEDVMASTVSSIPADLTLRRAVDDHFMRFD
                     HSAFPVVDGAETVGLLSLRRVKQVPRQEWDSRTVRETMGKLDEGCTVSPTTSMADVVE
                     RLEGENRRVVVVEDGEVVGIISPSDVARWLQRRQLIES"
     gene            5369..6148
                     /locus_tag="VF058_00690"
     CDS             5369..6148
                     /locus_tag="VF058_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003988618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX6206853.1"
                     /translation="MPELPDVEGYRRYLAHHAQGRRVERVHADPTILRNTTPQGLGRA
                     LVGGVLGPPRRHGKWLSCPVDGPTLVLHFGMTGSLVWSGEEPERHPYDRMILEVEGGE
                     LRYRDMRKLGGVWVVPRGVDVESVTGPLGPDAQRIDREDLVELLARRRGGVKAALMDQ
                     RMLAGLGNLLVDEILWRARVHPRRAIGDLGRGDLERIADRTEEVLRLSIELGRVPPDE
                     TWLMGSRDGRDATCPRCGTTLERVKAGGRTSVFCPRCQPWS"
     gene            6139..7032
                     /gene="ligD"
                     /locus_tag="VF058_00695"
     CDS             6139..7032
                     /gene="ligD"
                     /locus_tag="VF058_00695"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018890550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX6206854.1"
                     /translation="MELTIGERTIEMRNEGKVFFPDAGITKGDVVRYYARIAETMLPH
                     VRGRPISMERLPDGLGGTRWYQKEAPDHFPDWIERAEVPKKDGVVHHVVLREAADLVY
                     LATQGCLTPHVLLSTAEHLDRPDRMIFDLDPSVEDVGVLRDGARALRDLLEALELAPF
                     VMTSGSRGFHVTVPLAARDDTATVHRFAHDVAKLIVGDDPDRFTIEHRKAKRGDRVLV
                     DYFRNGWAQTAVPPYAVRARPGAPIAAPLDWEELGRTLPQRYTLRNAFRRLGRKPDPW
                     SDIGRHARGLRGARKLLDAMR"
     gene            complement(7035..7616)
                     /locus_tag="VF058_00700"
     CDS             complement(7035..7616)
                     /locus_tag="VF058_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6206855.1"
                     /translation="MPEGARGLVVFAHGSGSSRHSPRNQQVAEALQPRALGTLLFDLL
                     TPEEDEVDRRTASLRFDIPLLAERVEGAVSWVREEVPGLPVGLFGASTGAAAALVAAA
                     RVPGDVRAVVSRGGRPDLAGDWLPRVLAPTLLIVGSLDTEVLGMNRDAMARMERAEVT
                     LEVVEGASHLFEEPGKLERVGELAREWFAAHLT"
     gene            complement(7682..8101)
                     /locus_tag="VF058_00705"
     CDS             complement(7682..8101)
                     /locus_tag="VF058_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020524389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6206856.1"
                     /translation="MQTIADVMTKTPKTLEEDATLVDAAQLMRREDIGDVLVTRDGRC
                     TGIVTDRDIVVRAIAKGKLPGETPLSEVASEDVVTLSPSDAVAQAVAEMRDKAIRRLP
                     VVDEEGRPVGIVSIGDLAVERDADSALADISAAPPSD"
     gene            complement(8123..9079)
                     /locus_tag="VF058_00710"
     CDS             complement(8123..9079)
                     /locus_tag="VF058_00710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6206857.1"
                     /translation="MLEIGYTLSGEEFGPRDLVTFARRAEETGFSFALVSDHYHPWID
                     RQGHSPFVWGVLGGVSQATERLRIGTGVTCPTIRIHPAIIAQAAATAACMLPGRFFLG
                     VGSGENLNEHVLGDRWPAAAVRLDMLEEAIDVIRLLWEGETTSHRGEHYVVENARVYT
                     LPDEPPPIYVAASGPRSLRIAGGLGDGLIGVAPDPEMVETFRDAGGEGKPAYGQLHVC
                     WAENEQDAVRTALEWWPNTSLPGQLGQELPLPRHFEQAARLVTEDVIREQVVCGPDPE
                     RFLDAIREYERAGFDHVYLHQIGPDQEGFFRFCEAEILPKIG"
     gene            9157..9570
                     /locus_tag="VF058_00715"
     CDS             9157..9570
                     /locus_tag="VF058_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206858.1"
                     /translation="MEVTTAMLADAATVENGKLYVHGGAWSVINVRELPVTHPSMALA
                     LVFRVEYTEALEDHPIAIELLDEDDQPVGPRIDGIIHVGHPPRMRPGTPSFVPQAIRF
                     NNVRFEREGGYRFRVAVRDDEVASVQFRVLRTRGQ"
     gene            complement(9564..10709)
                     /locus_tag="VF058_00720"
     CDS             complement(9564..10709)
                     /locus_tag="VF058_00720"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206859.1"
                     /translation="MDLLRPRDLRTLVEAPPGLSVSIYMPSHRAGPQVRQDPIRLKNL
                     LGLAEERLLSDGLRRPEAREILDPAGDLLEDAEFWRHQGDGLALFLSKDHGLVLRLPV
                     AFEELLVTGDRYQIKPLIPLLSGDARFLILALSQKEIRLLQATRQVIEEVELEDVPES
                     LREAAVHREMRFLNYRVGTGFTSGGRQSAIFHGHGDRTDEEYVRKYLRAIDEGLRSAI
                     EGERSPLVLAGVRSLLDTYRTVSQYPDILPEIVQGNPDELSNEELHAQARPPVEARHR
                     QRIEDAVGRFEEQAGTGLAVSGVHEVVPAAAHGRIEVLWVGLGVQRWGSFDDETASVE
                     VRDQPKAGDEDLLDRAAADALLSGGTVYAVDPGEVPGDAPIAALLRY"
     gene            complement(10748..11188)
                     /locus_tag="VF058_00725"
     CDS             complement(10748..11188)
                     /locus_tag="VF058_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6206860.1"
                     /translation="MARTTAMEREAADLLGRTSLFSELSKRELLAIARAGKEVGRAEG
                     ATLAREGETGVGFFLILEGDATVSIAGRPAGVLTKGDTFGEVALLDGGPRTASVEATS
                     PVRLFGLTQWAFTRVVREHPTVALKLLQVLAGYVRNSASGLPQL"
     gene            complement(11231..12601)
                     /locus_tag="VF058_00730"
     CDS             complement(11231..12601)
                     /locus_tag="VF058_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEX6206861.1"
                     /translation="MRVDAQLLSEVRDRFEHAVLVFLDPEGYPMSVATAYSVDADSGV
                     VELERPAGALPAPDAEVNVIFSHIRPQPEMGYDERRYVQLWGRLSEAGGRLRLAPERA
                     HTWDEQKVPFFQYSEQTVPQAHRYMEDLSRRQGRRVKPRLSFGWLALRTTRAPFLSAT
                     IVPVLLGIAVAALQGSWDLGLALLTVLAASFIHLALNVANDVFDTRSGADAANTTPTK
                     FSGGSRVAHYGLVSLKGLALLSAGLYAAGIGIGLYLAVVTDFWPLFWLGVAGVAISVF
                     YTAPPFRLVHRGLGEIAVGLGFGPIMVLGAYFVQAERFSWEAGYASIPVAILISLILY
                     VNEIPDRRGDAAAGKRTLPVRWSKEAVIAAYVAAGAATYAAITGGAVLGIIPRPTILA
                     LATIPLAVKVARGLWEHYDSPYTLMGVMGVNIQLHALTGGLLILGYLIAIWADRLMDS
                     VPIFLR"
     gene            complement(12598..13086)
                     /locus_tag="VF058_00735"
     CDS             complement(12598..13086)
                     /locus_tag="VF058_00735"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6206862.1"
                     /translation="MGALPDPIEEVLNAALVCELSVVSSAGRPVTHPMIPLYDGERIY
                     MTSSALFSRKLDHIRANPKVSISISDLGATHGDPMGRRLTVQGDARILEDDPHTTWER
                     ILPLWIVKEPIVKSFYEQRVALPLFWERALIEVTPRRVLVWEDGDTSREPEIVELEEA
                     AR"
     gene            complement(13136..13732)
                     /locus_tag="VF058_00740"
     CDS             complement(13136..13732)
                     /locus_tag="VF058_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6206863.1"
                     /translation="MRRLFAFLATTIDGYHEGPKRELDWHNASEAEGSGFPIASGFPH
                     LGDPARDEIDTIVFGRVTYELMASYWPTREAEEADPEIAARMNGLQKVVVSRTLEGAD
                     WANTRLVRNAVAELTELKQAPGKDLGIFGSSTLTASLLPTGLIDELRILVNPVVLGAG
                     HPVLASAARASMTLAGCRTFPSGNVLLTYHPAADRGER"
     gene            13835..14434
                     /gene="rpsD"
                     /locus_tag="VF058_00745"
     CDS             13835..14434
                     /gene="rpsD"
                     /locus_tag="VF058_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX6206864.1"
                     /translation="MLKRGPRHRICRRLGVAVCGRPNCPVEKRPYPPGEHGRARRRET
                     EYQVRLAEKQKLRAIYGIGERQFRKYFAKASRRKGVTGEALLQALETRLDSLVLRLGL
                     ALSQRQARQLVSHGHVLVDGRRVNVPSAQVRPGQTITVADKARNFVSVREALEVASDL
                     PPYLSRTEAAGGRLERLPERDEIPLPVEVDERLIVEYYA"
     gene            14511..14894
                     /locus_tag="VF058_00750"
     CDS             14511..14894
                     /locus_tag="VF058_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6206865.1"
                     /translation="MATIKEAIGMLPVRDLEAAKSWWADRFSLKPTEDSDMGSYYEVG
                     STRFLVYPSQFAGSNQATAMGLHVDDVESTVEELRSKGVQFEEYDMPQIKTERGIATM
                     EEGGRTMQVAWFKDPDGNIISISNY"
BASE COUNT         2140 a         5268 c         5409 g         2099 t
ORIGIN      
        1 tcgcgccccc gccccccgcc tcctcgggta ccatccgggc gtggccgctc tgcaggagct
       61 cctcgactcc gcccccgagg accgccgccc caccctccgc gcggtccaca aggcgatccg
      121 cgccgccgcc cccaagctga agatgaagat ctggcggcag aagttctggg gcggcaccga
      181 ccagacgatc ctcgggtacg gcgacatcac ctaccggagc tcggcgggga aggacgtcga
      241 gtggttccgg ataggcctgg ccaaccagaa gagctacgtc agcgtctacg tcagcgccac
      301 ccgggaccgg cggtacctcc ccgagatcta cggcccccgc ctggggaagg tgaaggtcgg
      361 gaggtcctcg gtctcgttcc gaaagctgga ggacctcgat ctcgacgtcc tccgcgagat
      421 ggtggccgag gccgaccagg cctgagccac agaggcgaat agataagctt cactttccag
      481 gaatgtagga gtatgcttca gaacgacgat atgaaggaga tagctgcatc ggggaccctc
      541 tggggggcgg acgggctcgc cgcggtgatc ggcgacgtcg tcgggtccag agaggtcgag
      601 gaccggcgcc ggacgcagga cgagctggcg gacgcgctgg agggggtgaa cggtcgggtg
      661 ccggccaggt cgtcgctccg gcccacgatc ggggacgagt tccagggcct ctacgactcg
      721 ctgggcgacg ccctccatat cacgctcctc ctccggctgc tgctgaaggg gagggtcgag
      781 gtgcggttcg gggtcggctg ggggcacgtg gagttcttcg aggggaccac ccaggacggc
      841 cccggctggt ggggcgcccg cgaggccatc gagctcgtac gcgaagccca ggactcgaga
      901 ggcgagcctc gtggactgcg aacgatgttc gtcgcgcgat tcgcggagcg aagcgagctc
      961 gaagagaagg aagggctcct gcccgacctc gaaggcgtca tcaacgcggc gctggtcacg
     1021 cgggatgagc tcgtctccgc catggacgag cgggacgctc gcatcgcctt ggggctgatg
     1081 gaggggaaga cgaacgccgc gatcgccgag gaggagcagg tgtcgacctc ggccatctcc
     1141 cagcgggccc tcggcgcggg gatctacgcg gtccttcgag cggatcgcct cctccgctcg
     1201 gcgttcgagg gggcgccggc gtgagcctcc tcgcgctgtg gttcgccgcc gtgggactgg
     1261 ccgatctgac cacggggctc gccggcgcgg cggtgggcac ggccagggcc ctctccgccg
     1321 cggtcgtcgg tgcggtcgcc gcggccgtcg gcgctctcct gatcggtcac gagatcggcg
     1381 ccgtcctgtt cgccggcgtc gtggcgctgg cgctgctggt gggatgggcc tggctccgga
     1441 ccggcacctg gaccaacgag cgggtcgccg gcgccctggc gctgcacgcg acggccatcc
     1501 tggttctcgc gacgctctcg gggacgctgc ccgaaggaga cggcggggtc ttcgcccggt
     1561 ggctggaggg cctcccgttc gtcggggcct ccacgtggga gtcggggctg ctcgtcgtgg
     1621 gcatcgtgct gtggctgggc gcgaccgcga actcgatcgt ccgcatggcg atcttcctgg
     1681 tcgagaagca gatcgacctg cgggtggggg aggagcagct gaagggcggc cgcatcatcg
     1741 gcccgctcga gcgctggctg atcttctcgc tcgtgctctc ggggaacgcg gtggccgccg
     1801 ggttcatcac cgccgcgaag agcctggccc gcttccccga gctggggcgg agcgccaaca
     1861 tccggctgga caccgagtac ctgctggtgg gcagcctcgt cagctggaca ctggcgctcg
     1921 ccccgctcct gctcgtgccg tagggcctac tcgaccgtca gcgttccctc catcccggca
     1981 tcgcggtggc ccgggacgtc gcagtggaac gtgtagctgc ccgcctcgag ctcgatcgtg
     2041 cccgtgtcgg tctggccggg atcggcggag gccacgacct cgtcgccggc ctcctcgacc
     2101 acgaacgtgt gattgatgcc ctcctcggac gtcagctcca cggtgatcga gcccgcctcg
     2161 gcgctcagct cgctcggctg gaacgccagc gcgtcggtcc ccgtcaccga gacgtcggcg
     2221 cttccgcctc cgccggtggc cccgtcgtcg cctccgtcgt cccctccgca ggcgccgagc
     2281 accagcgcgc ccgcgaccag cgtgatcagc atgcgtgctc gcatcgttct ccttccgatc
     2341 gtcggatggg ccgtcgtggg gttcgcgggc ctccggacgg cctccccgat gctacggacc
     2401 tccctcgccg ccgtcagcgc cgatccttca ggtgccctcc gatcgtgccg aaggtggagg
     2461 catgagctgg cgcagcgact ccgcgggcct cgcgctcctc ctgacccgac cgacgggcga
     2521 acaggatcgc gatcgggctc gcgctggcga cgaccgccgc cgcgaggccc gccggggcgc
     2581 cccgcggcgt cggcgaccgc tccagacccg cgatcctcgt cgctgacccc accccggcgt
     2641 ccgggtagga tgccggacat gctgggagag ggcgcgcggg cgccggcgtt cacgcttccc
     2701 gaccaggacg gcaaccccat ctcgctgagc gactacgcgg gtcgatgggt ggtcttctgg
     2761 tggttcgtgc gggccgacac ccccgggtga acgatggagg gccaggggtt ccgtgatcga
     2821 gcccccgagt tcgaggagct gggcgccgag atcctggggg tctcctacga ccctcccgag
     2881 gcccagcggg ccttcgccga gaagcagggg ttcgccttca ggctcctctc ggacggggac
     2941 cggaccgtgg ccgcggccta cgggaccctg cggcccgacg accacgagcg tgcggcgttc
     3001 cccaggcgct tcacctacct gatcgacccc gcggggaggg tggcacgcgc ctacgtggtg
     3061 aaggacgtcg ccgcgcaccc gagggaggtc ctggaggatc tgcgggccct ggcccgcggg
     3121 gcatgacccg gggcagcgca ggcgtgctgc tcgacgtgga cggggtcctc gtcacgtcct
     3181 gggagccgct gcccggggcg gtggagacga tcgcgtggct gcgcaagtcg ggcgtcccct
     3241 tcctcctcgt cacgaacacc acgacccggt cgcgcgcctc gctggcccgg gacctcgtcc
     3301 ccttcgacgt gggcccggac gacctcctga ccgcgccgat ggccacggcc gcgtacctgc
     3361 gggccgagca cccgggggcc cgcgtccacc tgatggccac gggcgacgtg gccgaggacc
     3421 tcgaggggat cgagctggtg gacggtgacg cggacgtcgt ggtgatcggg ggagcgagcg
     3481 aacccgaccc cgagcatccc gacacgacgt tcacctacga gaacctcgac cgggcgttcc
     3541 gcatgctgct cgacggggcc cggctcgtgg cgatgcaccg caacctctcg tggaggacga
     3601 gccgcggcgt cgtgctcgac tcgggggcgt tcgtcgtggg tctggagcgg gcggcgaagg
     3661 tcgaggccga ggtcgtgggg aagccgtcgg cggccttctt ccgggcggcg ctggagcggc
     3721 tggggatgcc cgccgaccgc gcgctcatgg tgggcgacga cgtcgaggcc gacgtggtgg
     3781 gcgcgcggtc ggcgggcatc cggggggtcc tcgtccgcac cggcaagttc cgccccgagc
     3841 acctcgaggc gggcagcccc gaccacgtga tcgactcgat cgccgacctc ccgggcctcc
     3901 tcggggggta ggcccgccgc gtatgggccg aacggccgat accctctctc gccatacgtc
     3961 gtaaggttgg gattcgagac gagagggtag gccccggatg cccatgaacg aggacccgct
     4021 caagaggcgc ctccgcgatc gggacttcgc ccgcgcctac cgctcctggg tgaggctcct
     4081 ggtggatcgc gcgaagcacc gcggatcgac gcgccgcgtg gtccggctgc ccagggacgg
     4141 gtcgctgaga ccgcccaagt cgctcgccgg ctgactcagg actcgatcag ctggcggcgc
     4201 tggagccagc gcgccacgtc cgacggcgag atgatgccca cgacctcgcc gtcctccacc
     4261 acgaccaccc ggcggttctc gccctccagc cgctccacga cgtcggccat cgaggtcgtg
     4321 ggcgagaccg tacagccctc gtcgagcttc cccatcgtct ctcgcaccgt cctcgagtcc
     4381 cactcctggc gcggcacctg cttcacgcgg cgcaggctga gcagccccac ggtctcggcg
     4441 ccgtccacga ccgggaacgc gctgtggtcg aagcgcatga agtgatcgtc cacggcccgc
     4501 cgcagggtga ggtcggcggg gatcgagctg acggtggagg ccatcacgtc ctcggcgtcg
     4561 acgtcctcga gcatccgccg gagctggagc tgctcgtagc tcatgcgagc ggcctgggcc
     4621 aggaaccagc cgatcgcccc gaaccagatc cccgcgccga gcgccccgtt gaacacgaag
     4681 aagaggccgg cggccacgag caggtacccg aagccctgcc cggcgatgct ggcgatccgg
     4741 gtcgccttgc tgaggctgcc cgtggcgccc cagatgatcg cgcgcagcac ccgcccgccg
     4801 tcgagcggga agccggggac gaggttgaag acggccaggg ccaggttgac gaacgccagg
     4861 tagccgaacg cgccggccac ctcccgaggg aggaaggcat cgcccagggt cccgaggagg
     4921 gcgaagatcc ccgccagcac caggctcgac agcggcccca ccagggcgat gaggaactcc
     4981 gcgccggggc ccttggcctc gaccttggcg tgggtggcgc cgccgaagag gaacagcgtg
     5041 atgctcttca ccgggatgcc ccgcgactgc gccgtgaacg cgtgggcgaa ctcgtggaag
     5101 acgaccgagc cgaagaagag gatggccgag ccgacgccca gcaggatggc gtaggcgttg
     5161 tcgatgcccc ggtagatctc gaggtaccgc cagtacagcg agtaggagat gagaacggcg
     5221 atgatggccc aggagatgtc gatgcggagc tcgatgccgc ggatgcggcc cacgcgccag
     5281 gagttgccga acatggcccc agcgtacccg actccatccc cggcgaacgg ggagcgtggg
     5341 cggccggggt cgcgggaagg tcaccgggat gcctgagctg cccgacgtcg aagggtaccg
     5401 tcgctacctg gcccaccacg cccaggggag acgcgtcgag cgggtccacg ccgaccccac
     5461 catcctcagg aacaccaccc cccagggcct ggggcgggcg ctcgtgggcg gcgtgctcgg
     5521 cccgccccgg cgccacggga agtggctctc gtgcccggtg gacggcccga cgctcgtcct
     5581 gcacttcggc atgacgggca gcctcgtctg gtcgggggag gagcccgagc gccaccccta
     5641 cgaccggatg atcctggagg tcgagggcgg cgagctccgc taccgcgaca tgcggaagct
     5701 ggggggcgtc tgggtggttc cccggggcgt ggacgtcgag tccgtcaccg gccccctggg
     5761 tcccgacgcc cagcgcatcg accgggagga cctcgtggag ctcctggcgc gcaggcgggg
     5821 gggcgtgaag gcggcgctga tggaccagcg gatgttggcc gggctgggga acctcctggt
     5881 ggacgagatc ctgtggcggg cccgggtcca ccccaggcgg gccatcgggg acctgggccg
     5941 cggggacctg gagcgcatcg ccgatcgcac cgaggaggtg ctccgcctct cgatcgagct
     6001 gggtcgggtg ccgccggacg agacgtggct catggggagc cgcgatgggc gagacgcgac
     6061 ctgtccccgc tgcggcacga cgctggagcg ggtcaaggcc ggggggagga cctcggtctt
     6121 ctgcccgagg tgccagccgt ggagctgacg atcggcgagc ggacgatcga gatgcggaac
     6181 gagggcaagg tcttcttccc ggacgccggc atcacgaagg gggacgtcgt ccggtactac
     6241 gcgcggatcg ccgagacgat gctgccccac gtgcggggcc ggccgatctc gatggagcgg
     6301 ctccccgacg gcctgggcgg gacccgctgg taccagaagg aggcgcccga ccacttcccc
     6361 gactggatcg agcgggcgga ggtccccaag aaggacggcg tcgtgcacca cgtcgtactg
     6421 cgggaggccg ccgacctcgt ctacctggcc acccaggggt gcctcacccc gcacgtcctg
     6481 ctgtcgaccg ccgaacatct cgaccggccc gatcggatga tcttcgacct ggacccctcg
     6541 gtcgaggacg tgggcgtgct ccgggacggg gcccgggccc ttcgggacct gctggaggcg
     6601 ctcgagctgg cgcccttcgt gatgacgagc ggctcgcggg gcttccacgt gacggtgccg
     6661 ctggcggctc gggacgacac ggcgaccgtc caccgcttcg cccacgacgt ggccaagctg
     6721 atcgtcgggg acgaccccga ccggttcacg atcgagcacc gcaaggcgaa gcgaggagac
     6781 cgcgtgctcg tggactactt ccggaacggc tgggcgcaga cggccgtccc gccctacgcg
     6841 gtgcgggccc ggcccggcgc gccgatcgcg gcgcccctcg actgggagga gctggggcgg
     6901 acgcttcccc agcggtacac gctccggaac gccttccggc ggttgggccg caagcccgac
     6961 ccgtggagcg atatcgggcg gcacgcacga ggcctgcggg gggcgaggaa gctcctcgac
     7021 gcgatgcgct gacgtcaggt caggtgggcc gcgaaccact cccgggccag ctcgccgacc
     7081 cgctccagct tccccggctc ctcgaacagg tgggaggcgc cctccacgac ttcgagggtc
     7141 acctcggctc gctccatccg ggccatcgcg tcgcggttca tgcccagcac ttcggtgtcg
     7201 agggagccca cgatcagcag ggtgggggcg aggacgcggg ggagccagtc gccggcgagg
     7261 tcggggcggc cgccgcgcga caccaccgcg cgcacgtcgc ccgggacgcg cgcggccgcg
     7321 accagcgcgg cggccgcccc ggtgctggcc ccgaacagcc ccacgggcag gcccggaacc
     7381 tcctcccgga cccacgacac ggcgccctcc acccgctcgg ccagcagggg gatgtcgaag
     7441 cgcagggacg ccgtcctccg gtcgacctcg tcctcctcgg gcgtcaggag atcgaagagg
     7501 agcgtcccca gagcccgcgg ctggagggcc tcggccacct gctggttcct cgggctgtgg
     7561 cggctggacc cgctcccgtg ggcgaagacg acgaggccgc gggcgccctc cggcacgtcg
     7621 aggaaccccc cgacgcggac cggacccgcc tcgacctcga tctcgtccgt cccctgcctc
     7681 ctcagtcgct cggcggcgcg gcgctgatgt cggccagggc cgagtcggcg tcgcgctcca
     7741 cggcgaggtc cccgatcgag acgatcccca cgggccggcc ctcctcgtcc acgacgggga
     7801 gccggcggat cgccttgtcc cgcatctcgg ccacggcctg ggccacggcg tcggatggcg
     7861 agagcgtcac gacgtcctcg ctggccacct cgctcagggg cgtctcgccg gggagcttgc
     7921 ccttcgcgat cgcccggacc acgatatcgc ggtcggtgac gatcccggtg cagcgcccgt
     7981 ccctggtgac gaggacgtcc ccgatgtcct cccgacgcat cagctgcgcc gcgtcgacca
     8041 gggtggcgtc ctcctccagg gtcttcggcg tcttcgtcat cacgtcagcg atggtctgca
     8101 tcgtccttcc ctccttcggg gatcagccga tcttggggag gatctccgcc tcgcagaacc
     8161 ggaagaagcc ctcctgatcg ggccctatct ggtggaggta cacgtggtcg aaccccgccc
     8221 gctcgtactc gcggatcgcg tccaggaacc gctcggggtc ggggccgcag acgacctgct
     8281 cccggatcac gtcctcggtc acgagccggg cggcctgctc gaagtggcgg gggagcggaa
     8341 gctcctggcc cagctggccg gggagcgagg tgttgggcca ccactcgagc gccgtgcgga
     8401 ccgcgtcctg ctcgttctcg gcccagcaca cgtggagctg gccgtaggcg ggcttcccct
     8461 cgccccccgc atcgcggaag gtctcgacca tctccgggtc cggggccacc ccgatcaggc
     8521 cgtccccgag gcccccggcg atccggagcg agcggggtcc cgaggccgcc acgtagatcg
     8581 ggggcggctc gtctggcagc gtgtacacgc gggcgttctc cacgacgtag tgctctcccc
     8641 gatggctcgt ggtctcgccc tcccacagca ggcggatcac gtcgatcgcc tcctcgagca
     8701 tgtcgagccg tacggcggcc gccggccacc ggtcgcccag cacgtgctcg ttgaggttct
     8761 cgccgctgcc cacgccgagg aagaagcgac ccggcagcat gcaggcggcg gtggccgccg
     8821 cctgggcgat gatcgccggg tggatccgga tcgtggggca ggtgaccccg gtgcctatcc
     8881 ggaggcgctc ggtcgcctgg gagacgcctc cgagcacccc ccacacgaac gggctgtggc
     8941 cctggcggtc gatccacggg tggtagtggt ccgagacgag cgcgaacgag aagccggtct
     9001 cctcggcccg ccgggcgaac gtcaccaggt cccggggccc gaactcctcg ccggagagcg
     9061 tgtagccgat ctcgagcatg ccgtgtgggt acccgcgggc ggggggttcc atgcgacggg
     9121 gtggcggatc ggccctcctg cgggaatcat gggggcgtgg aggtcacgac ggccatgctc
     9181 gccgatgccg ccaccgtgga gaacggcaag ctctacgtcc acggcggggc ctggagcgtg
     9241 atcaacgtcc gggagctgcc ggtgacccac cccagcatgg ccctggccct cgtgttccgc
     9301 gtggagtaca cggaggcgct ggaggaccac ccgatcgcga tcgagcttct ggacgaggac
     9361 gaccagccgg tcggcccgcg gatcgacggg atcatccacg tcggccaccc gccccggatg
     9421 cggccgggca ccccctcgtt cgtcccgcag gcgatccgct tcaacaacgt ccgcttcgag
     9481 cgcgagggcg gataccgctt ccgcgttgcg gtccgggacg acgaggtggc gtcggtgcag
     9541 ttccgggtcc tgcggacccg gggtcagtag cggaggaggg cggcgatcgg cgcgtcgccg
     9601 gggacctcgc ccgggtccac cgcgtagacc gtccctcccg agagcagcgc gtcggccgcg
     9661 gcccggtcca gcaggtcctc gtcgccggcc ttcggctggt cccgcacctc gaccgacgcc
     9721 gtctcgtcgt cgaaggagcc ccaccgctgg accccgagcc cgacccacag cacctcgatg
     9781 cggccgtggg cggccgcggg caccacctcg tgcacgccgc tcaccgcgag cccggtgccg
     9841 gcctgctcct cgaaccgccc caccgcgtcc tcgatcctct gacggtgccg ggcctccacg
     9901 gggggccggg cctgcgcgtg cagctcctcg ttcgagagct cgtcggggtt cccctggacg
     9961 atctccggga ggatgtcggg gtactggctg acggtccggt acgtgtccag aagggagcgg
    10021 acgccggcca gcacgagggg cgagcgctcg ccctcgatcg ccgaccggag cccctcgtcg
    10081 atcgcccgca ggtacttccg gacgtactcc tcgtcggtcc ggtcgccgtg gccgtggaag
    10141 atcgccgact ggcgtccgcc gctcgtgaag ccggtaccca cgcggtagtt caggaagcgc
    10201 atctcgcggt ggacggccgc ctcgcggagc gactcgggca cgtcctccag ctcgacctcc
    10261 tcgatcacct ggcgggtggc ctggagcagc cggatctcct tctggctcag cgccaggatc
    10321 aggaagcggg cgtcgccgga gagcaggggg atcaggggct tgatctggta ccggtcgccg
    10381 gtcaccagga gctcctcgaa cgccacgggc agacggagca cgagcccgtg gtccttcgac
    10441 aggaagagcg ccagcccgtc cccctggtgc cgccagaact ccgcgtcctc gaggagatcc
    10501 cccgcggggt cgaggatctc ccgcgcctcc ggacggcgca gcccgtcgga cagcagccgc
    10561 tcctcggcca gacccaggag gttcttcagg cggatcgggt cctgccgcac ctgcgggccc
    10621 gcccggtggc tgggcatgta gatggagacg gagaggcccg ggggcgcctc gacgagggtt
    10681 cggaggtctc gcgggcggag cagatccatg tcgggcaggg taccccgggt cggtgacggc
    10741 tacgcgctca gagctgaggg agccccgaag cggagttgcg gacgtacccg gcgagcacct
    10801 gcaggagctt cagcgccacg gtcggatgct cgcggaccac ccgggtgaag gcccactggg
    10861 tgaggccgaa gagccgcacg gggctcgtgg cctcgacgct cgcggtccgg ggcccgccgt
    10921 cgaggagggc cacctccccg aaggtgtcgc ccttggtgag gacgcccgcg ggcctgccgg
    10981 cgatcgagac cgtggcgtcc ccctccagga tcaggaagaa gccgacgccg gtctcgccct
    11041 cccgggccag ggtggcgccc tccgcccggc cgacctcctt gccggcccgg gcgatcgcca
    11101 gcagctcgcg cttggagagc tccgagaaga ggctggtgcg gccgaggagg tcggccgcct
    11161 cccgctccat cgccgtcgtc ctcgccatcc gcgcgcctcc ctcggtcgct cggattctac
    11221 gccgccgggg ctaccgcagg aagatgggga ccgagtccat gagccggtcg gcccagatcg
    11281 cgatcagata gccgaggatc aggagccctc cggtgagggc gtggagctgg atgttcacgc
    11341 ccatcacgcc catcagggtg tagggcgagt cgtagtgctc ccacagtccg cgggccacct
    11401 tcaccgccag cgggatcgtg gctagggcca ggatcgtggg ccgggggatg atcccgagga
    11461 cggccccgcc ggtgatcgcg gcgtacgtcg cggccccggc cgcgacgtac gccgcgatca
    11521 ccgcctcctt cgaccaccgc acgggcaggg tccgcttccc ggcggctgcg tccccgcgcc
    11581 ggtcggggat ctcgttcacg tagaggatca gcgagatcag gatcgccacg gggatcgagg
    11641 cgtacccggc ctcccacgag aagcgctcgg cctgcacgaa gtaggcgccc agcaccatga
    11701 tcggcccgaa ccccaggccc acggcgatct cgcccagccc ccggtggacg aggcggaagg
    11761 gaggggcggt gtagaagacg ctgatcgcca cgccggccac ccccagccag aacagcggcc
    11821 agaagtcggt gacgacggcc aggtacagcc cgatcccgat cccggccgcg tagaggcccg
    11881 ccgagagcag ggccagcccc ttcaggctga cgaggccgta gtgcgccacc cgggagcctc
    11941 cgctgaactt cgtcggggtg gtgttcgccg cgtccgcccc cgatctcgtg tcgaagacgt
    12001 cgttcgccac gttgagcgcg aggtggatga acgaggccgc cagcaccgtg agcagcgcca
    12061 gccccaggtc ccacgagccc tggagcgccg cgacggcgat gcccaggagg accgggacga
    12121 tcgtggccga gaggaagggg gcccgggtcg tgcgcagggc gagccagccg aacgagagcc
    12181 ggggcttcac ccgccgcccc tgccgccggg agaggtcctc catgtagcgg tgcgcctgtg
    12241 gcacggtctg ctcggagtac tggaagaacg gcaccttctg ctcgtcccag gtgtgggccc
    12301 gctcgggggc gagccgcagc cgcccgcccg cctcactgag gcgcccccag agctggacgt
    12361 accgccgctc gtcgtagccc atctcgggct ggggccggat gtggctgaag atcacgttga
    12421 cctcggcgtc gggggcgggc agcgcgccgg ccgggcgctc cagctccacc actcccgagt
    12481 ctgcgtcgac cgagtaggcc gtggccacgc tcatcgggta gccctcggga tcgaggaaga
    12541 cgagcacggc gtgctcgaag cggtcgcgga cctccgacag cagctgcgcg tcgaccctca
    12601 tcgcgccgcc tcctcgagct cgacgatctc gggctcgcgc gaggtgtcgc cgtcctccca
    12661 gacgagcact cggcgcggcg tgacctcgat cagggcccgc tcccagaaca gcggcagcgc
    12721 cacgcgctgt tcgtagaagc tcttcacgat cggctccttg acgatccaca gcggcaggat
    12781 ccgctcccag gtcgtgtggg ggtcgtcttc gaggatgcgc gcgtcgccct gaacggtgag
    12841 gcggcgcccc atcgggtcgc cgtgcgtcgc gcccaggtcg ctgatcgaga tcgagacctt
    12901 cgggttggcg cggatgtggt cgagcttgcg actgaacagc gcgctcgacg tcatgtagat
    12961 ccgctcgccg tcgtagaggg ggatcatcgg gtgggtcacc gggcggcccg cggagctcac
    13021 caccgagagc tcgcagacga gggccgcgtt caggacctcc tcgatcgggt cgggcagggc
    13081 tcccacgtcg tcctccaatg atcggatgtc cgcggggaca gagtaaccga gggcgtcacc
    13141 gttcgccgcg gtcggccgcc ggatggtagg tgagcaggac gttccccgag gggaaggtcc
    13201 tgcatcccgc cagggtcatc gaggctcgcg ccgccgaggc cagcaccgga tggccggccc
    13261 ccagcacgac cgggttcacg aggatgcgga gctcgtcgat caagccggtc ggcagcagcg
    13321 aggcggtgag cgtcgagctt ccgaagatcc cgagatcctt gccgggcgcc tgcttgagct
    13381 cggtgagctc ggcgacagcg ttccggacga gtcgggtgtt ggcccagtcc gccccctcga
    13441 gggtgcgtga gacgacgacc ttctggagcc cgttcatccg ggcggcgatc tccggatctg
    13501 cttcctccgc ctcgcgggtg ggccagtatg acgccatcaa ctcgtaggtg acccgcccga
    13561 agacgatcgt gtcgatctcg tcgcgggcgg ggtcgccgag gtgggggaag cccgatgcga
    13621 tagggaagcc gctcccctcc gcctcggagg cgttgtgcca gtccagctcg cgcttcgggc
    13681 cttcgtgata cccgtcgatc gtcgtggcca ggaacgcgaa cagccgtctc atccgtctgc
    13741 tccttccgtc ggtgcggtaa tggagacctc tgggggagcg ttggctcatc gcccgtagtc
    13801 gcggaacacc ctcctgaggt agcatccttt gtccatgctg aagcgaggcc cacgtcaccg
    13861 catctgccgc cgcctgggcg tggccgtctg cggacggccc aactgcccgg tggagaagcg
    13921 accgtacccc cccggcgagc acggtcgggc ccgtcgccgt gagaccgagt accaggtccg
    13981 cctagccgag aagcagaagc tccgcgcgat ctacgggatc ggggagcggc agttccggaa
    14041 gtacttcgcc aaggcgtcgc ggcgcaaggg cgttaccggc gaggccctgc tgcaggccct
    14101 ggagacgcgc ctcgacagcc tggtccttcg cctcgggctg gctctgagcc agcggcaggc
    14161 ccgccagctc gtctcgcacg gccacgtgct ggtcgacggg cgccgggtga acgtgccgag
    14221 tgcccaggtc cggccgggcc agacgatcac ggtggcggac aaggcccgca acttcgtgtc
    14281 ggtccgagag gcgctcgagg tcgcgtccga ccttccgccc tacctgagcc ggaccgaggc
    14341 cgcgggcggc cgtctggagc ggctgcccga gcgcgatgag atcccactgc cagtcgaggt
    14401 ggacgagcgc ctcatcgtcg agtactacgc ctgagcacgg gccccttcca tcgatccgtt
    14461 gacgtcttca tcgacccgcc catagggtcg agacgaggag gtggttcgcg atggcgacca
    14521 tcaaggaagc gatcgggatg ctcccggtga gggacctgga ggccgcgaag agctggtggg
    14581 ccgaccggtt cagcctcaag ccgaccgagg acagcgacat gggctcgtac tacgaggtgg
    14641 gctccacccg cttcctcgtc tatcctagcc agttcgccgg gtcgaaccag gccaccgcga
    14701 tggggctcca cgtcgacgac gtcgagtcga cggtcgagga gctccgttcg aagggggtgc
    14761 agttcgagga gtacgacatg ccccagatca agaccgagag gggcatcgcc accatggagg
    14821 agggcggccg gacgatgcag gtcgcgtggt tcaaggaccc ggacgggaac atcatctcga
    14881 tctcgaacta ctgagatcca ggggggcggc gcgggc
//
LOCUS       DASUTW010000019         2681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773 k141_7428,
            whole genome shotgun sequence.
ACCESSION   DASUTW010000019 DASUTW010000000
VERSION     DASUTW010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7428"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VF058_00755"
     CDS             complement(<1..657)
                     /locus_tag="VF058_00755"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6206866.1"
                     /translation="MTDEERPEATEEPGAGPGTIEEPEDRSEGAAGPGPGETSFEAPR
                     AGGALRSLLVPVLAFITALILGGLLMVFTDQEALRAWASFVRSPIYALKVSGQVVLEA
                     YQALFTGSLGSPIGIVEALASGDLEEIQVAFRPLSETIVAATPLIFAGLSVALGFRAG
                     LFNIGAEGQMNAGAITAAFVGFTFTGLPVGIHVIVAVAAGFLGGALWGAIPGILKART
                     G"
     gene            complement(654..2177)
                     /locus_tag="VF058_00760"
     CDS             complement(654..2177)
                     /locus_tag="VF058_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6206867.1"
                     /translation="MRLELRGITKRFPGVLANDRIDLDVSGGEIHALLGENGAGKTTL
                     MNVLFGLYHADEGEILIDGESVRFETPRDAIAAGIGMVHQHFMLIPVFTVTENVMLGV
                     EETGAVGLLDTREARRRVEEISGRYGLEVDPDARIEDLSVGAQQRVEIVKALYREAEV
                     LILDEPTAVLTPQETEELFGVMRTLRESGVGIIFITHKLKEVLAVSDRITVLRRGRVA
                     GATTPKEADESKLASMMVGRDVRLEVEKEPAQPGDPVLQVEDLTVLDDRGQVAVNGIS
                     FEVRNGEVLGVAGVQGNGQTELVEAITGLRPVDAGRVMIGGKDLTRASPREVFLAGVG
                     HIPEDRINDGLVADFPVADNLILDQYYQRPFARRWIRDDEAIHRAAEQRREAYDIRTP
                     SVDQPVSNLSGGNQQKVIVARELSREIALLVASQPTRGLDVGSMAYIHEQVIAARDGG
                     TAVLLVSSELDEVLSVADRVAVIYRGRIVGLVDPKTIGREEIGLMMAGATAGQAEGS"
     gene            complement(2249..>2681)
                     /locus_tag="VF058_00765"
     CDS             complement(2249..>2681)
                     /locus_tag="VF058_00765"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6206868.1"
                     /translation="DFENQDNGRRVTEDLISEGADVILPVAGPVGLGTAAAVEDSGQE
                     DVMVGVDTDWCVSAEEACPIVLTSVMKNMDVAVFNAVQSVVDGSFEGGLYVGTLSDEG
                     VGLAPFHEYEGEISEDVVAALEELRAGIIDGSISVNGVDYL"
BASE COUNT          393 a         1015 c          864 g          409 t
ORIGIN      
        1 gcccgtgcgc gccttgagga tcccgggtat cgcgccccag agcgccccgc ccaggaaccc
       61 ggcggccacc gccacgatga cgtggatccc gacggggagg cccgtgaacg tgaagcccac
      121 gaacgcggcg gtgatcgccc cggcgttcat ctggccctcg gcgccgatgt tgaacagccc
      181 cgcccggaac cccaaggcca ccgacagacc cgcgaagatc agcggggtcg cggcgacgat
      241 cgtctccgac agcggccgga acgcgacctg gatctcttcg aggtcgccgg aggcgagcgc
      301 ctccacgatc ccgatcgggc tgcccagcga gcccgtgaac agggcctggt aggcctcgag
      361 cacgacctgc cccgaaacct tcagcgcgta gatcgggctg cggacgaagc tggcccacgc
      421 ccggagagcc tcctggtccg tgaagaccat cagcagtccc ccgaggatga gcgcggtgat
      481 gaaagccagc acggggacga gcagcgagcg gagggcgccg ccggcccgcg gcgcctcgaa
      541 gctcgtctcg cccggaccgg ggccggcggc gccctccgag cggtcctcgg gctcctcgat
      601 cgtgccgggg cccgcccccg gctcctcggt cgcctcgggt cgttcctcgt ccgtcatgac
      661 ccctccgcct gcccggcggt cgcgccggcc atcatcagtc cgatctcctc ccgtccgatc
      721 gtcttcgggt ccacgaggcc cacgatgcgc cccctgtaga tcacggccac gcggtcggcg
      781 acggagagca cctcgtcgag ctcgctggag acgagcagca cggcggtgcc cccgtcgcgg
      841 gcggcgatca cctgctcgtg gatgtaggcc atggatccca cgtcgagacc ccgcgtgggc
      901 tgagaggcca cgaggagggc gatctcgcgg gagagctcgc gggccacgat caccttttgc
      961 tggttgccac ccgacaggtt cgagacgggc tggtccaccg acggcgtgcg gatgtcgtag
     1021 gcctcacgcc gctgctccgc cgcccggtgg atcgcctcgt cgtcccggat ccatctccgg
     1081 gcgaaaggcc gctggtagta ctggtcgagg atgaggttgt cggccacggg gaagtccgcg
     1141 acgagcccgt cgttgatccg gtcctcgggg atgtgcccca cgccggccag gaagacctcc
     1201 cgcgggctgg cgcgcgtcag gtccttcccg cctatcatca cgcgtcccgc gtccaccggc
     1261 ctgagcccag tgatggcctc gaccagctcg gtctgcccgt tcccctggac cccggccacg
     1321 cccagcacct cgccgttcct gacctcgaac gagatgccgt tcaccgcgac ctgtccccga
     1381 tcgtcgagca ccgtcaggtc ctccacctgg aggacggggt ccccgggctg cgccggctcc
     1441 ttctcgacct cgaggcgcac gtcccgcccc accatcatcg aggcgagctt cgactcgtcg
     1501 gcctctttgg gcgtggtcgc ccccgccacc cgcccccgcc ggagcaccgt gatgcggtcg
     1561 gataccgcca ggacctcctt cagcttgtgg gtgatgaaga tgatccccac gcccgactcc
     1621 cgcagggtcc tcatcacccc gaagagctcc tcggtctcct ggggcgtgag cacggccgtg
     1681 ggctcgtcga ggatcagcac ctcggcctca cggtagagcg ccttgacgat ctccacgcgc
     1741 tgctgggccc cgaccgagag atcctcgatg cgggcatccg gatccacctc caggccgtag
     1801 cggccggaga tctcctcgac gcggcggcga gcctcgcgcg tgtcgaggag gccgacggcc
     1861 ccggtctcct ccacgcccag catcacgttc tcggtcaccg tgaagaccgg gatcagcatg
     1921 aagtgctggt ggaccatccc gatgcccgcg gcgatcgcgt cccgcggggt ctcgaagcgg
     1981 acggactcgc cgtcgatcag gatctcgccc tcgtcggcgt ggtacaggcc gaagaggacg
     2041 ttcatgaggg tcgttttccc ggcgccgttc tctccgagga gggcgtggat ctcaccgccc
     2101 gacacgtcga ggtcgatccg gtcgttggcc agcaccccgg ggaatcgctt ggtgatcccc
     2161 cgaagctcca gcctcatgcc ctccccctcg gaacggcgga ggggggcggc gggttcgccg
     2221 tcgcccccct cgctgccatc atcgttctct acaggtagtc gacgccgttc accgagatcg
     2281 aaccgtcgat gatgcccgcc ctgagctcct ccagggccgc caccacgtcc tcggagatct
     2341 cgccctcgta ctcgtggaac ggagcgaggc ccacaccctc gtccgagagc gtcccgacgt
     2401 agagaccacc ctcgaacgag ccgtccacca cggactgcac cgcgttgaac acggccacgt
     2461 ccatgttctt catgacgctg gtcagcacga tcgggcaggc ctcctcggca ctgacgcacc
     2521 agtcggtgtc gaccccgacc atcacgtcct cttgaccgga gtcctccacc gccgcggctg
     2581 tgccgaggcc gacgggcccc gcgaccggca ggatgacgtc ggcgccctcc gagatcaggt
     2641 cctcggtcac ccggcgaccg ttgtcctggt tctcgaagtc g
//
LOCUS       DASUTW010000020         6304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_655326, whole genome shotgun sequence.
ACCESSION   DASUTW010000020 DASUTW010000000
VERSION     DASUTW010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655326"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VF058_00770"
     CDS             complement(<1..456)
                     /locus_tag="VF058_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX6206869.1"
                     /translation="MPERDRPWIYRTYAGFGTPGATNERFRANLDKGQTGLSIAFDLP
                     TQCGYDPGHPMSVGEVGRVGVPISHLGDMRTVLEGIPVAEMNTSMTINATAVWLLALY
                     VAVADEHGASRPELRGTTQNDILKEYLSRGTFIFPPEPSMRLTTDLITWT"
     gene            complement(499..1659)
                     /locus_tag="VF058_00775"
     CDS             complement(499..1659)
                     /locus_tag="VF058_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX6206870.1"
                     /translation="MNSLSRRRQGFPRLRRGLALAVGLMLLAQVAPGSAQTTEEKLQA
                     AKKRVAQIQRQLELERAKLDRLNAEITRINRQIAQAYAQRIAIQEQIKLTEEAIQRKG
                     RRIRTLQGRLDDRAREVYIQGPAGMLEFVLEAESLSDLSDRVSFLSALSEGDASTAIG
                     IEVEREELGRFEDDQGVLLEEQDALLAQLRDQEEQLNARWEEQAAIEASIAEKLDEAQ
                     GLVQRLKKQRRRELLAALRAAAGSVRPVDASGLLKWCPVDRPRSYIDDFGFPRSGGRT
                     HQGNDIFAPEGTPIRAPFAGVADEGNDGLGGIAVFVRAPDGTYVYNAHLSRHAGVDGK
                     QVKPGDLIGYVGNTGNAAGTPPHDHFQLSPGGGSPVNPYPFLNAVCGINGGG"
     gene            complement(1762..2709)
                     /locus_tag="VF058_00780"
     CDS             complement(1762..2709)
                     /locus_tag="VF058_00780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX6206871.1"
                     /translation="MAAESRTTVLAALFANAVIAVAKLVGGLISGSSAMLAEAAHSMA
                     DTTNQGLLLMSISLGKRKPDERHPFGYGKERFFWAFVAAVFIFVAGSAFSLFEGVRAI
                     GAPHGEGETGFAIPYVVLSVALVVEAIALWVAYRGVRREATRRRRRFFSFLRVTRDPT
                     AKTALFEDAAAVTGVMVALVGVGLHERTGDPRWDAGASIVIGVILAIVAFVLARDTKA
                     LLLGEPAFPEERELLRECMLAEDGVEHVVELLTMAMGPESLLVTARLDLREGLDEDAI
                     EAMAHSIDRRCREAVPAVKELFLDPTSRDEVRIEDDGGV"
     gene            complement(2772..3941)
                     /locus_tag="VF058_00785"
     CDS             complement(2772..3941)
                     /locus_tag="VF058_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012786399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6206872.1"
                     /translation="MDFDLTEEQQEFRKLVRQFAEEVVAPRAEEMDQAGELPMDIVKQ
                     MGEMGLFGLPFPEEYGGQGADFLTLCIAIEELARVDSSIAITLEAAVGLGASPIYHRG
                     TEEQKQRWLVPMARGEIIGAYGLTEAGGGSDAEATRTTARVEDGEWVINGSKAFITNS
                     GTPISQVCTITAVTGEAEDGGKEISAIIVPTDTPGFEIGKSYRKVGWRASDTHELAFN
                     DVRVPEENLLGERGEGYAQFLQTLDEGRIAVAALAVGLTQGCLEESARYAKEREAFGR
                     PIGAFQGVQFKVADMKVAAETARLATYKAAWLRDQGRPYKAEASLAKLYASEVAVTAA
                     REAVQVHGGYGFIEEFPVARFYRDSKILEIGEGTSEIQRILIARDLGLPGTEFDD"
     gene            4021..4914
                     /locus_tag="VF058_00790"
     CDS             4021..4914
                     /locus_tag="VF058_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6206873.1"
                     /translation="MSETVIEVRGLRKSYGEVEAVAGLDLTVHRGEVMALLGPNGAGK
                     TTTVEILEGHRDRDEGEISILGHDPEERSPDLRRRVGIVLQTTGVEPYLTVGETIEQF
                     RGFYPSPLHLDEVLEMVGLSDLRDRRVKKLSGGQQRRLDVAIGLAGNPELLFLDEPTT
                     GFDPSARRGAWDMIRNLRSLGKTVLLTTHYMDEAQSLADRVAILVHGQIVAEGPPAIL
                     TGDGRLTTIRFRGSAEGLPPEVGEPEIDDGMVRIHTTEPTRALHVLTAWAAARGEELP
                     ELTVTKPSLEDVYLELTEGSS"
     gene            4911..5684
                     /locus_tag="VF058_00795"
     CDS             4911..5684
                     /locus_tag="VF058_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6206874.1"
                     /translation="MSEARLAARQILYENRAFWRNPPAAFFTFAFPLIFLVIFNMLFG
                     NEEIEVPGGRTTASTFFVPAIATFAIVNATFTNLATSIPFARDQGLLKRYRGTPLPGW
                     AYLTARVAHAIVVTILLVGIVTAFGALLYGVDAPTNTLPGVLVALAVGGFAFCSLGLA
                     MTAIIPNADAGPAIVNAVILPLLFISDVFIQTDQAPAWVSTLGSVFPVKHLSEALQTA
                     FNPFHAGAGFEWGHVGIVAAWGVAGLILAIRFFSWEPRT"
     gene            complement(5689..>6304)
                     /locus_tag="VF058_00800"
     CDS             complement(5689..>6304)
                     /locus_tag="VF058_00800"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX6206875.1"
                     /translation="VFDHEGLLRASARSRPRKTPLPSLGQGSRGKVVRLLEQRLRSLG
                     YWLTGVNRAFDHRTGDAVLAFHKVQGMPRVKSVRPATWRRLASPRRPRPRSTKPAFHV
                     EIDQTKQVLYVIRRGEISEIVHTSTGAGGATRDGVFRVHRKIAGYSPGRLYYPSYFDG
                     LRAVHGWPSVPTYPASHGCSRVPNWTAIHLHNVMPIGTVVRVYH"
BASE COUNT          888 a         2278 c         2162 g          976 t
ORIGIN      
        1 cgtccacgtg atcagatcgg tcgtcagccg catcgagggc tccggcggga agatgaacgt
       61 cccccgcgac aggtactcct tcaggatgtc gttctgcgtc gtgccccgga gctcggggcg
      121 cgaggccccg tgctcgtcgg ccacggccac gtacagcgcg agcagccaaa ccgcggtggc
      181 gttgatcgtc atcgaggtgt tcatctcggc caccgggatc ccctcgagca ccgtgcgcat
      241 gtcgccgagg tgcgagatcg ggacccccac cctgcccacc tcgcccaccg acatcgggtg
      301 gcccgggtcg tagccgcact gcgtggggag gtcgaaggcg atcgagaggc ccgtctggcc
      361 cttgtccagg ttggcccgga agcgctcgtt cgtggcgccc ggcgtcccga accccgcgta
      421 ggtgcggtag atccagggac gatcccgctc aggcatgcgt tgagtctacc cgccgccccc
      481 cggtcagccg cccgccggtc agccgccgcc gttgatgccg cagacggcgt tgaggaaggg
      541 gtaggggttg acgggcgagc cgccccccgg gctcagctgg aagtggtcgt ggggcggggt
      601 tcccgcggcg ttccccgtgt tgccgacgta gccgatcagg tcgcccggct tcacctgctt
      661 cccgtccacg ccggcgtgcc gggagaggtg cgcgttgtag acgtaggtcc cgtccggcgc
      721 ccgcacgaag accgcgatcc cgccgagccc gtcgttgccc tcgtccgcta cgccggcgaa
      781 cggggcgcgg atcggggtgc cctcgggcgc gaagatgtcg ttcccctggt gggtccggcc
      841 gcccgagcgg gggaacccga agtcgtcgat gtaggagcga ggacgatcca ccgggcacca
      901 cttgaggagc ccgctggcgt ccaccggcct gacgctcccc gcggcggccc ggagcgcggc
      961 cagcagctcc cggcgacgct gcttcttcag ccgctggacg agcccctggg cctcgtcgag
     1021 cttctccgcg atcgaggcct cgatggccgc ctgctcctcc caccgggcgt tgagctgctc
     1081 ctcctggtcc cggagctgag ccagcagggc gtcctgctcc tcgagcagca ccccctggtc
     1141 atcttcgaag cgccccagct cctcccgctc gacctcgatc ccgatcgccg tgctggcgtc
     1201 gccctcgctg agcgccgaca ggaagctcac ccggtcgctg agatccgaga gcgactcggc
     1261 ctccaggacg aactcgagca tccccgccgg cccctggatg tagacctcgc gggctcggtc
     1321 gtcgaggcgt ccctggagcg tgcggatgcg gcggcccttc cgctggatcg cctcctccgt
     1381 gagcttgatc tgctcctgga tcgcgatccg ctgggcgtat gcctgggcga tctgccgatt
     1441 gatcctcgtg atctcggcgt tgaggcggtc gagcttggcc cgctccagct ccagctggcg
     1501 ctggatctgc gccacgcgct tcttggccgc ctgcagcttc tcctcggtcg tctgggcgga
     1561 cccgggagcc acctgggcga gcagcatcaa cccgacggcg agcgcgaggc cccgccgcag
     1621 gcgggggaag ccttgacggc gcctgctcag cgagttcacg aacggttacc tctacaggaa
     1681 tcgtgttgcg actgtcaatc gctggtctca gcgtgacggc atccggccgc cggagcaagt
     1741 atcggccgga accggccccc actacactcc gccgtcgtcc tcgatacgga cctcgtcccg
     1801 ggaggtcggg tcgaggaaga gctccttgac ggcggggacg gcctcccgac accgacggtc
     1861 gatcgagtgg gccatggcct cgatcgcgtc ctcgtccagg ccctcgcgca ggtccagccg
     1921 ggccgtgacg agcagcgatt caggccccat ggccatcgtc agaagctcga cgacgtgctc
     1981 caccccgtcc tcggccagca tgcactcgcg gagcagctcc cgctcctcgg ggaaggccgg
     2041 ctcccccagc agcagcgcct tcgtgtcgcg ggccagcacg aaggccacga tggcgaggat
     2101 cacgccgatc acgatcgagg cgcccgcatc ccaccgcggg tcgccggtcc gctcgtggag
     2161 gccgacgccc acgagggcca ccatcacgcc ggtcaccgcg gccgcgtcct cgaacagcgc
     2221 ggtcttcgcc gtcgggtccc gggtcacgcg gaggaacgag aagaaccggc ggcggcgccg
     2281 agtcgcctcc ctccgcacgc cccggtaggc cacccacaag gcgatcgcct ccacgacgag
     2341 cgccacgctc aggacgacgt acgggatcgc gaagcccgtc tccccctcgc cgtggggggc
     2401 gccgatcgcc cgcacgccct cgaagaggga gaaggccgaa cccgccacga agatgaacac
     2461 ggccgcgacg aacgcccaga agaaccgctc cttcccgtag ccgaacggat gccgctcgtc
     2521 gggctttcgc ttccccagcg agatcgacat caggaggagc ccctggttcg tggtatcggc
     2581 catcgagtgt gcggcctcgg ccagcatcgc cgaggatccg gagatgagcc cgccgacgag
     2641 cttggccacc gcgatcacgg cgttggcgaa gagcgccgcc aggaccgtgg tcctgctctc
     2701 ggcggccatc cagctctccc ccggctacgt gcgccggctc tcctcgtcgc tcacgagccg
     2761 gaggacgggc atcagtcgtc gaactccgtg cccggaagac cgaggtcccg ggcgatcagg
     2821 atccgctgga tctcgctcgt cccctccccg atctccagga tcttcgagtc tcgatagaag
     2881 cgggccaccg ggaactcctc gatgaagccg tagcctccgt ggacctgcac cgcctcgcgg
     2941 gccgcggtga ccgcgacctc cgaggcgtac agcttggcca gcgaggcctc ggctttgtag
     3001 ggacggccct ggtcgcggag ccacgcagcc ttgtaggtgg cgaggcgggc cgtctcggcg
     3061 gccaccttca tgtcggcgac cttgaactgc acgccctgga acgctccgat cgggcggccg
     3121 aacgcctcgc gctccttcgc gtaccgggcc gactcctcga ggcacccctg cgtgagcccg
     3181 acggcgaggg cggcgaccgc gatccgcccc tcgtcgaggg tttggaggaa ctgggcgtag
     3241 ccttcccccc gctcccccag caggttctcc tcgggaacgc ggacgtcgtt gaaggccagc
     3301 tcgtgggtgt cgctggcgcg ccatcccacc ttccggtacg acttgccgat ctcgaagccc
     3361 ggggtgtcgg tggggacgat gatcgcggag atctccttgc cgccgtcctc ggcctcgccg
     3421 gtgaccgcgg tgatcgtgca gacctgggag atcggcgtgc ccgagttcgt gatgaaggcc
     3481 ttggagccgt tgatcaccca ctcgccgtcc tcgacgcggg ccgtcgtcct cgtggcctcg
     3541 gcgtcggagc cgcccccagc ctcggtgagc ccgtacgcgc cgatgatctc tccccgggcc
     3601 atcgggacca gccaacgctg cttctgctcc tcggtgccgc ggtggtagat cgggctggcc
     3661 ccgagcccga ccgccgcctc gagggtgatg gcgatcgagg agtccacgcg ggccagctcc
     3721 tcgatcgcga tacagagcgt caggaagtcg gcgccctggc cgccgtactc ctcggggaac
     3781 gggagcccga acagccccat ctcgcccatc tgcttgacga tgtccatcgg gagctcgccg
     3841 gcctggtcca tctcctcggc gcgcggggcc acgacctcct ccgcgaactg gcggacgagc
     3901 ttgcggaact cctgctgctc ctcggtcaga tcgaagtcca tccagtccct cctgagatcg
     3961 gatcgagtct accggaggca cgcggtggag ccgcgggtcc catcgcgcta gcctggaggt
     4021 atgagcgaga ccgtgatcga ggtccgtggg ctgcggaagt cctacgggga ggtggaggcc
     4081 gtcgcgggcc tcgacctgac ggtccaccgc ggtgaggtca tggcgctgct gggacccaac
     4141 ggcgcgggga agacgaccac cgtggagatc ctcgaggggc atcgggaccg cgacgagggc
     4201 gagatctcga tcctgggcca cgacccggag gagcgatcgc ccgatctccg acgccgcgtc
     4261 gggatcgtcc tgcagacgac cggcgtggag ccgtacctga ccgtggggga gacgatcgag
     4321 cagttccgcg gcttctatcc gtcaccgctc cacctggacg aggtcctgga gatggtcggg
     4381 ctgtcggacc tccgcgatcg gcgcgtgaag aagctctccg gcggccagca gcggcggctc
     4441 gacgtggcca tcggcctcgc cgggaacccc gagctgctct tcctggacga gccgaccacc
     4501 ggcttcgatc cttcggcccg ccgcggagcc tgggacatga tccggaacct ccgctcgctg
     4561 gggaagaccg tgctcctcac cacgcactac atggacgagg cgcagagcct ggccgaccgc
     4621 gtggcgatcc tcgtccacgg tcagatcgtg gccgaggggc cgccggcgat cctgacgggc
     4681 gacggccggc tgacgacgat caggttccgg ggatcggcgg agggtctgcc gcccgaggtg
     4741 ggcgagccgg agatcgacga cggcatggtc cggatccaca cgaccgagcc cacccgcgcg
     4801 ctgcacgtgc tgaccgcctg ggccgccgcg cggggcgagg agctgcccga gctcacggtg
     4861 acgaagccct cgctggagga cgtgtacctc gagctgacgg agggatcctc gtgagcgagg
     4921 ctcggctggc cgcgcgacag atcctctacg agaaccgggc cttctggcgg aacccgccgg
     4981 cggcgttctt caccttcgcc ttcccgctga tcttcctcgt gatcttcaac atgctgttcg
     5041 ggaacgagga gatcgaggtg cccggaggcc ggaccactgc gtcgaccttc ttcgtccccg
     5101 cgatcgccac cttcgccatc gtcaacgcga cgttcacgaa cctggcgacg tcgatcccgt
     5161 tcgcccgcga ccagggccta ctgaagcgct accgggggac gccgctcccc ggatgggcct
     5221 acctcaccgc gcgcgtcgcg cacgcgatcg tcgtcacgat cctgttggtg gggatcgtca
     5281 cggcgttcgg cgccctgctc tacggcgtgg acgccccgac gaacaccctg cccggcgtgc
     5341 tggtggcgct cgcggtgggc gggttcgcgt tctgctcgct ggggctggcc atgacggcga
     5401 tcatcccgaa cgcggatgcg ggacccgcca tcgtgaacgc ggtgatcctg ccgctgctct
     5461 tcatctcgga cgtgttcatc cagaccgacc aggcccccgc ttgggtgagc accctgggca
     5521 gcgtcttccc cgtgaagcat ctgtccgagg ccctccagac ggcgttcaac ccgttccacg
     5581 ccggggccgg gttcgagtgg gggcacgtcg ggatcgtggc ggcgtggggc gtggccgggc
     5641 tgatcctggc gatccggttc ttctcctggg agccgcggac ctagcgcgtc agtggtagac
     5701 gcgcaccacc gtgccgatcg gcatcacgtt gtgcaggtgg atcgcggtcc agttcgggac
     5761 ccgtgagcac ccgtggctcg ccgggtaggt cggcaccgac ggccatccgt gcaccgcgcg
     5821 cagcccgtcg aagtacgagg ggtagtagag acggcccggg ctgtaccccg cgatcttccg
     5881 atggacccgg aacaccccgt cccgcgtcgc gccgccggcc ccggtcgagg tgtggacgat
     5941 ctcgctgatc tccccccgcc tgatcacgta gagcacctgc ttcgtctggt cgatctcgac
     6001 gtggaaggcg ggcttcgtgg agcggggcct gggccgcctg ggcgacgcga gccgccgcca
     6061 ggtggccggg cgcacggact tcacccgggg catcccctgc accttgtgga aggcgagcac
     6121 cgcgtccccc gtccggtgat cgaaggcgcg gttgacgccc gtcagccagt agccgagcga
     6181 gcggagccgc tgctcgagga ggcgcacgac cttcccccgc gacccctgcc ccagggaggg
     6241 gagcggcgtc ttccgggggc ggctccgcgc gctcgcccgc aggagcccct cgtggtcgaa
     6301 gacg
//
LOCUS       DASUTW010000021        11528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_396105, whole genome shotgun sequence.
ACCESSION   DASUTW010000021 DASUTW010000000
VERSION     DASUTW010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11528
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396105"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..3613
                     /locus_tag="VF058_00805"
     CDS             83..3613
                     /locus_tag="VF058_00805"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEX6206876.1"
                     /translation="MKRSDFGPNTWLVEQMYRRYQENPSSVSEAWQEFFEDYVPEADG
                     QRRPASAREEAPPEPEPKEEVPEEAVPLRGAAKVIAENMDRSLGVPTATSFRTVPAKL
                     LELNRGMLNRHLGRNRGGKASFTHLIGFAVVRALAGRPAMNVGYREIDGIPHAIRYPH
                     VNLGLAVDVQKEDGSRTLLVPNIKEAETLGFEAFWRSYEELVGKVQRNELSPDDFAGT
                     TVSITNPGMLGTVQSVPRLMPGQAAIIGVGAIGFPAEYSGADPDTLAELGVGKVLTLT
                     STYDHRVIQGAESGEFLRDVERLLLGEERFYDEVFASMGVPYVPVEWRRDVNPRPGTL
                     GADEKEARVSQLINMYRVRGHLIANLDPLESEPPAMHPELDPAFFGFTIWDLDRRFPT
                     GGLAGTEETTLGEILSVLREAYCRTSSVEYMHMQDVEPKRWIQGRVEGRQADLTKEDR
                     THILSKLNQAEAFEQFLHTKYVGHRRYGLEGAESMIPMLDAILDLAADSAIEETLIGM
                     AHRGRLNVLANVIGKSYAQIFRQFEGDIDPLSAQGSGDVKYHVGAAGKHTSRAGNQLV
                     VSVVSNPSHLEAVDPVLEGMARAKLEQLDRDDEDPVLPLLIHGDAAFAGQGVVAETFA
                     LSQLNGYSTGGTVHIIVNNQLGFTTGSDYGRSSTYATDVAKMVQAPIFHVNGDDPEAC
                     VRVARLAFAFRETFHRDVVIDMWCYRRWGHNEGDEPAFTQPRMYAKIRSLRSVRKRYM
                     EQLVNRGLLTVEEAERSLEEYRETLQQALDETKAESQPQRPSAREAREPEPAPPIETG
                     VERAVLDEVIEGITTVPETFHVHEKLQRWLDQRRKALPEDAVDWSLAEALAFGSLIRD
                     GVWIRLSGQDTRRGTFSQRHSVLVDQETGKEYLPLANLEGAPGRFVALDSLLSELAAL
                     GFEYGYSVSNPGALVLWEAQFGDFANGAQVVIDQFIASAEDKWGQTSGLVMLLPHGFE
                     GQGPEHSSARLERYLTLAAKDNMEIAVPSTPAQYFHLLRRQALRDRRKPLIVLTPKSP
                     LRLPAARSAADEFTLGRFREVLPDPVEPSEIRRVLLVQGKFFYDLLRGRGEREVDDVA
                     LVRVEQLYPFPAAALREQLDRYPGAEVVWAQEEPENMGAARFVHLKMERLLDVRPRLV
                     ARPESPSPATGSQTVHQHEQTELIDRSLSD"
     gene            4032..5264
                     /locus_tag="VF058_00810"
     CDS             4032..5264
                     /locus_tag="VF058_00810"
                     /inference="COORDINATES: protein motif:HMM:NF039610.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5979 domain-containing protein"
                     /protein_id="HEX6206877.1"
                     /translation="MMTMTNNYRHRTAAGVTRRWLAILVVLLVALGSPLAAWAATDGD
                     EPEVTAAEATDEPAEEPPAEEAPAEEAPAEEAPAEEPEPAPEPEPEPPANPDGQSGAV
                     EAAQTKGGGGGGETAAQAEALADEPGATSANGVTPTFVEGNPSCPAGTTELKVEPPAA
                     GQYTDGTLIVNMTTSDTPPNGSIGWTSNIGIDYVIVKGGPNASLYSYSPESTGDSGLT
                     SPFNPNNGQYYGVSHVSFCYDVEETGSLDVAKVVDGTGSPEPGTEFTIDVDCDDDAYD
                     ATLVFDEDGNLTSGSLPITGIPLGTECTVTETGTGDADSVTYSPDGGTPSDPPTVEIT
                     SDGQEVTVTVTNTFIEVLGDITDRDTDTDTDTDVTTTEEGGTLAFTGAELLILALMAL
                     ALVLVGTPLVILTRRRSE"
     gene            complement(5333..5812)
                     /locus_tag="VF058_00815"
     CDS             complement(5333..5812)
                     /locus_tag="VF058_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX6206878.1"
                     /translation="MLSLAHPRIKRIPILLLLAALLLSLMAYGEAEAATKAQRVERRL
                     MNQERRPKARATFRLDRGLSRIARRHSRRMASMGTLHHNPNLVEQVRGRRWQRLGENV
                     GVVGPTGNLRETLRMLHGAFMNSDPHRRNILYRPFRRVGVGLVRNDGRVWVTIVFLG"
     regulatory      complement(5866..5943)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(6014..6283)
                     /locus_tag="VF058_00820"
                     /pseudo
     CDS             complement(6014..6283)
                     /locus_tag="VF058_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018791175.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
     gene            complement(6494..7141)
                     /locus_tag="VF058_00825"
     CDS             complement(6494..7141)
                     /locus_tag="VF058_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6206879.1"
                     /translation="MARWATFDCYGTLIDWDRGIGDALAGLWPRADRRRLLAGFHAVE
                     PLIQDAEPTLPYRKVLDRARVAVGAVLGLPAGEENVLAESLPTWPPFPEVPGVLRDLR
                     DRGWRLAILSNTDPDLLAASLERIAVPFELTVTAADAGSYKPSPGHWERFDALSGDRE
                     SHVHVGASPFHDLEPCARLGIPAIWINRLGQRSDLPRAAELPTLEGLPEALEVVA"
     gene            complement(7175..7675)
                     /locus_tag="VF058_00830"
     CDS             complement(7175..7675)
                     /locus_tag="VF058_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206880.1"
                     /translation="MRRVIVILALLAAACSGEAPAEPAPEATASGVPYPYPFTTPTPP
                     PEVTPLDGVWIREVPDELAGPVGKCRRCPPYRLELGDTNTLTIVRGTFEIEHTLHGWT
                     NAGHVFVEADTATLINDPNCTSAEGVYRWTIEGDELTFELVEDACAFGDLRPRYFTST
                     SWTRAG"
     gene            7761..8459
                     /locus_tag="VF058_00835"
     CDS             7761..8459
                     /locus_tag="VF058_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX6206881.1"
                     /translation="MITRVFRGTVRPGQHAEFERLFREEAVPRFETTPGCLSVHFGTP
                     TEASPDEYMIVTVWEDLDSLKGFAGEQWSEPKLSLHEAHVLADATVHHYRNGDGETIV
                     PPASLRGVGRPAEVLDLGRLRVDLARRVAELDGRVVELPPREFSVLSELALRAGEPVP
                     SDELARLVWPEDAWPTGEDVRRSVYRLRRLLGDRSRARPLIRTRRGHGYVLDPGGDGT
                     SPGHGRHSRTAARA"
     gene            8473..8838
                     /locus_tag="VF058_00840"
     CDS             8473..8838
                     /locus_tag="VF058_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206882.1"
                     /translation="MRATAVATVLVLASCARLPDPSTLPGEYESTTAPATLTLKDGEW
                     TLDNGGFVKSGAYGISGDRVAFLITETSHPAFDNYCREEADVYEWRFDDDLLVLRAVG
                     DTCDPVGQSVLTHGPWERA"
     gene            8835..9104
                     /locus_tag="VF058_00845"
     CDS             8835..9104
                     /locus_tag="VF058_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206883.1"
                     /translation="MSWEVRAARVMAWVTLVTGGGLGIACFVLAPAKVTTTERIVGHA
                     TITYEGVELVVRESFILAGIASLVLGAALWALLLLAARIASARDG"
     gene            9167..11107
                     /locus_tag="VF058_00850"
     CDS             9167..11107
                     /locus_tag="VF058_00850"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HEX6206884.1"
                     /translation="MRALRALAAMGILLASCTSGPVTGLGGIPQLLAGGLQPFDDCED
                     FLSYVKGHGLEHVTAWGLGGGWGPVFFDAEVAAEGEFRAAQPTAGVDYSTTNIQTSGV
                     DEPDIVKTDGERLLALVRGRLFVFDVSGSAPRKVGSLRLGDVWMRDMVLHGDRALLLG
                     EGWGGGPGPEPLRRDVVSHGGSISLLMEVDLTDPSDPEIVRRLYLDGRYLSARIADGI
                     VRVVVSSFPVGLEFEFPGERGGLQAKREALEHNRGVIRASTVEDWVPHHVLEDGDGTV
                     LAEGPLLDCASAHHPTEFAGFDMLTVLTLDLEEGLSASATEGSVGVLSAGETVYASPE
                     SLYVATQRWIDPIAFERGMPADLREDFTTQIHRFDISEDEPATYRSSGRVPGWLLNQF
                     SMDEHEGRLRVASTDSPEFWATQKAESVVTVLEERAGALVPVGSVGGLGEGERIYAVR
                     FMGDVGYVVTFRQVDPLYVVDLSDPATPAVTGELKIPGYSAYLHPVGEGRLLGVGQDA
                     TEGGLTRGTQLSLFDVSDPADPRRIDTVKIADGSSEAEWDHHAFLYWEPEGLVVVPIT
                     TWGDELGGGGFSGAVAVRLQGDGLWKAGTLSHGDGAVGEWLPGGIRRSLVVGDLLYTV
                     SETGLEASDLQTLDDVAWIPFR"
     gene            complement(11142..>11528)
                     /locus_tag="VF058_00855"
     CDS             complement(11142..>11528)
                     /locus_tag="VF058_00855"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6206885.1"
                     /translation="ANGLSNLGVGYGRVGRWDESRTAIHEALELFSAARNPTGIASVI
                     LDLAFILSWQGRHEDAVRLAGAWEVARDAAGGGPPSDFLGALIGDPVQEAKDALPDEA
                     ARRAWDEGKALNWDEAVSLARELATD"
BASE COUNT         1766 a         4015 c         4083 g         1664 t
ORIGIN      
        1 ccgccgagtg cctgagattc cgagggggcc ggcgtgacgg tcctgggtag gatggagaag
       61 gaagcaacga aggcgaggct gcgtgaagcg ttccgatttc ggccccaaca cctggctggt
      121 ggagcagatg taccgccggt accaggagaa cccgagctcc gtcagcgagg cctggcagga
      181 gttcttcgag gactacgtcc ccgaggccga cggccagcgc cggcccgcct ccgcccgcga
      241 ggaggccccg ccggagcccg agcccaagga ggaggtcccc gaggaggccg tcccgctccg
      301 cggcgcggcc aaggtcatcg ccgagaacat ggaccggagc ctgggggtcc ccacggccac
      361 gtcgttccgc acggtccccg ccaagctgct cgagctcaac cgaggcatgc tcaaccggca
      421 cctcggccgg aaccgggggg gcaaggcctc gttcacccac ctgatcggat tcgccgtggt
      481 gcgggccctc gccgggcggc cggcgatgaa cgttggctac agggagatcg acgggatccc
      541 ccacgcgatc cgctacccgc acgtgaacct ggggctcgcc gtcgacgtcc agaaggagga
      601 cgggagccgc actctcctcg tcccgaacat caaggaggcc gagaccctcg gcttcgaggc
      661 gttctggcgg tcctacgagg agctggtcgg caaggtccag cggaacgagc tctcgcccga
      721 cgacttcgcc ggcacgaccg tctcgatcac gaacccgggg atgctcggga ccgtccagtc
      781 cgtcccccgg ctgatgccgg gccaggccgc gatcatcggc gtgggggcga tcggcttccc
      841 cgccgagtac tcgggggccg acccggacac cctggcggag ctgggcgtgg gcaaggtcct
      901 caccctcacc tcgacctacg accaccgcgt gatccagggc gccgagtccg gcgagttcct
      961 cagggacgtg gagcggctcc tcctgggcga agagcgcttc tacgacgagg tcttcgcctc
     1021 gatgggcgtg ccctacgtcc ccgtcgagtg gcgccgggac gtgaaccccc ggccgggcac
     1081 gctcggcgcc gacgagaagg aggcccgcgt ctcccagctc atcaacatgt accgggtgcg
     1141 cggccacctc atcgcgaacc tcgacccgct cgagtccgag ccgcccgcga tgcacccgga
     1201 gctcgacccc gcgttcttcg gcttcacgat ctgggacctc gaccgtcgct tccccaccgg
     1261 cgggctcgcg ggcaccgagg agacgaccct cggcgagatc ctgagcgtcc tgcgcgaggc
     1321 ctactgccga acctccagcg tggagtacat gcacatgcag gacgtggagc ccaagcggtg
     1381 gatccagggg cgggtggagg ggcggcaggc cgacctcacg aaggaggacc ggacccacat
     1441 cctctcgaag ctgaaccagg ccgaggcgtt cgagcagttc ctccacacga agtacgtggg
     1501 ccatcgccgc tacggactgg agggcgccga gagcatgatc ccgatgctcg acgcgatcct
     1561 cgacctggcc gccgactcgg cgatcgagga gacactgatc gggatggcgc accgcgggcg
     1621 cctcaacgtg ctggccaacg tgatcgggaa gtcgtacgcg cagatcttcc ggcagttcga
     1681 gggggatatc gaccccctgt ccgcgcaggg atcgggggac gtgaagtacc acgtcggggc
     1741 ggccggcaag cacacctcgc gcgccggcaa ccagctcgtg gtctcggtgg tctccaaccc
     1801 ctcgcacctg gaggccgtgg accccgtgct cgaggggatg gcccgggcca agctcgagca
     1861 gctcgatcgc gacgacgagg acccggtgct ccccctcctg atccacgggg acgccgcctt
     1921 cgccggccag ggggtcgtgg ccgagacctt cgctctctcc cagctcaacg ggtactcgac
     1981 cggcgggacc gtccacatca tcgtcaacaa ccagctcggg ttcaccaccg gctcggacta
     2041 cggtcgctcc tcgacctacg ccaccgacgt ggccaagatg gtccaggccc cgatcttcca
     2101 cgtgaacggc gacgaccccg aggcctgcgt ccgcgtggcg cggctggcct tcgccttccg
     2161 ggagaccttc catcgcgacg tcgtgatcga catgtggtgc taccggcggt ggggccacaa
     2221 cgagggcgac gagcccgcgt tcactcagcc gcggatgtac gcgaagatcc ggagcctccg
     2281 ctcggtgcgc aagcgctaca tggagcagct cgtcaaccga ggcctcctca ccgtcgagga
     2341 ggcggagcgc tcgctcgagg agtaccggga gacccttcag caggcgctgg acgagacgaa
     2401 ggccgagtcc cagcctcagc gcccgtcggc gcgggaagcg cgcgagccgg agccggcgcc
     2461 cccgatcgag acgggggtcg aacgtgccgt gctcgacgag gtgatcgagg ggatcaccac
     2521 ggtccccgaa accttccacg tccacgaaaa gctccagcgg tggctcgatc agcggaggaa
     2581 ggcccttccc gaggacgccg tggactggtc gctcgccgag gccctcgcct tcgggtccct
     2641 gatccgggac ggcgtgtgga tccggctctc ggggcaggac acacgccggg ggaccttcag
     2701 ccagcgtcac tccgtgctcg tcgaccagga gaccgggaag gagtacctgc cgctcgcgaa
     2761 ccttgaggga gcgcccgggc gcttcgtcgc gctggacagc ctgctctccg agctcgccgc
     2821 gctgggcttc gagtacggct actcggtgtc gaacccgggg gccctcgtcc tctgggaggc
     2881 gcagttcggg gacttcgcca acggggccca ggtcgtgatc gaccagttca tcgcctcggc
     2941 cgaggacaag tggggccaga cgagcggcct cgtgatgctg ctgccccacg gcttcgaggg
     3001 ccaggggccc gagcactcct ccgcccgcct ggagcgatac ctcacgctgg cggcgaagga
     3061 caacatggag atcgcggtgc catccacccc cgcgcagtac ttccacctgc tgcggcggca
     3121 ggccctccgc gaccggcgca agcccctgat cgtgctgacg ccgaagtccc cgctccgcct
     3181 cccggcggcc cgcagcgcgg ccgacgagtt cacgctgggg cgcttccggg aggtcctgcc
     3241 cgatcccgtc gagccgtccg agatccgccg cgtgctcctc gtccagggga agttcttcta
     3301 cgacctcctg cgcggccgcg gggaacggga ggtcgacgac gtcgcgctgg tccgggtcga
     3361 gcagctgtac ccgttccccg cggccgccct ccgcgagcag ctcgatcgct acccgggggc
     3421 cgaggtcgtg tgggcccagg aggagcccga gaacatgggc gctgcgcgct tcgtccacct
     3481 caagatggag cggctcctcg acgtccgtcc ccggcttgtg gcccgacccg agagtcccag
     3541 tccggcgacc gggagccaga ccgtgcatca gcacgagcag acagagctca tcgaccggtc
     3601 cctctcggac taggctcgtc tagcaccttc ggccagagtc agtagttctt agtaactatg
     3661 tctatttagc tctggcgggt ggtcctcact cggtctgtcg gctgccaaac tcgtaatagt
     3721 ccattccccc gtatggggga gcttccccca cgagggggac gtagcaggac gagccgtact
     3781 ggcgcccgcc ccgccacccg ccgttggagc cccagctccg gcggtggcgg aacgtccctg
     3841 gtcagcaaga aggagggagc gggatgcagg acacggatcg ccgagggccc ccggcgcacg
     3901 caagcggggc cgttatccgg tgaacccagg cgcacaccgt gatgagccga atgtcccgaa
     3961 cgatgggggt ctcgaggccc ccgttggggc gttcggggga cacgatactt gctcttgggg
     4021 gatagagagg gatgatgaca atgactaaca attaccgaca caggaccgcg gccggcgtca
     4081 cgcgccgctg gctcgccatc ctggtcgtac ttctggtggc gctgggcagc ccgctggctg
     4141 cctgggccgc gaccgatggg gacgagccgg aggtgacggc cgccgaggcg accgacgaac
     4201 cggcagagga gcctccggca gaggaggctc cggcggagga ggctccggct gaggaggctc
     4261 cggctgagga gccggagccg gctcccgagc ccgaacccga gccaccggcc aaccccgacg
     4321 gtcagtccgg cgccgtggag gcagcccaga cgaagggcgg cggtggcgga ggcgagacgg
     4381 cggctcaggc cgaggcgctg gccgacgagc ccggcgcgac ctccgcgaac ggcgtcaccc
     4441 cgacgttcgt cgagggcaac ccgtcgtgtc ccgccgggac gacggagctc aaggtcgaac
     4501 cgcccgccgc gggccagtac acagacggca cgctcatcgt gaacatgacg acgagtgaca
     4561 cgcccccgaa cgggtccatc ggttggacct cgaacatcgg gatcgactac gtcatcgtca
     4621 agggcggccc gaacgccagc ctctacagct actcgccgga gtccaccggc gacagcgggc
     4681 tgacctcccc gttcaacccg aacaacgggc agtactacgg cgtgagccac gtctcgttct
     4741 gctacgacgt cgaggagacg ggctcgctgg acgtggccaa ggtcgtcgac gggaccggct
     4801 cgcccgagcc ggggaccgag ttcaccatcg acgtggactg cgacgatgac gcctacgacg
     4861 cgacgctggt cttcgacgag gacgggaacc tcacctcggg gagcctcccc atcacgggga
     4921 tcccgctggg gaccgagtgc acggtgaccg agaccgggac cggggacgcc gattcggtga
     4981 cctactcccc ggacggcggc accccctctg accccccgac cgtcgagatc accagcgacg
     5041 gccaggaggt cacggtgacg gtgaccaaca ccttcatcga ggtcctgggg gacatcacgg
     5101 accgggacac ggacacggac accgacaccg acgtcacgac cacggaggag ggaggcacgc
     5161 tcgcgttcac cggagcggag ctgctgatcc tggcgctgat ggcgctggcg ctcgtcctgg
     5221 tcgggacgcc gcttgtgatc ctcacgaggc gccggagcga gtaacccaac ccgctccgcg
     5281 aggagcggga gcgcccccga ggccttcggc ctcgggggcg ctcccgcctc cctcagccca
     5341 ggaagacgat ggtcacccag acccgcccgt cgttccggac gagccccaca cccacccttc
     5401 ggaagggtcg gtagaggatg ttccgacggt gggggtcgct gttcatgaac gcgccgtgga
     5461 gcatgcggag cgtttcccgg agattaccgg tgggtccgac gacgccgacg ttctcgccca
     5521 gccgctgcca ccgacggccc cggacctgct cgacgaggtt cgggttgtgg tggagggttc
     5581 ccatactcgc catccggcgg ctgtgccggc gcgcgatcct ggagagcccg cggtcgagcc
     5641 tgaacgtggc acgggccttg gggcggcgct cctgattcat cagcctgcgt tcgacgcgct
     5701 gggccttcgt ggccgcctcc gcctctccgt acgccatcag ggagagaagg agcgcggcca
     5761 ggagcaggag gatcgggatc cgcttgatcc ttggatgcgc gagcgaaagc atcggtggac
     5821 acctcctcgc ggtcccgcca ggacccgcga gtggtgtcct ggtccttcgg caggtcggct
     5881 gcccattggc gggggtccgt acctttgcgt cccgtcgtca ccgacggttt gcctttatcg
     5941 agaccgttcg tcgtctttgg ttgattgtgc tcaccctatg tatcggagga tggagcgcga
     6001 tgcttgagct ggctcagcga gagaggacca cgacgcggac ctcgaccagg cccttctcga
     6061 gtggcgccag ccgggcgaac gccgccctgg agagatcgag gaaccggcct tcgacgaagg
     6121 gcccccgatc ggtgaccgtc acggtcacgc cgcggccccc gtgctccacc cggagccggg
     6181 tgccgcaggg gagttggcgg tgggcggtcg tgagcgcgtc gggatcgtag ggatcaccgc
     6241 aggccgtcgt cctcccccgg aaggtctccc cgtaccacga ggcgacgccg aagagctcgg
     6301 ggcgctggag agccggcgcc gcccctcgag gaggctcgtc ccgcgaggcc ggctccgggt
     6361 acgcgaagcc cgtgatcgcg gcgagcgcga tcaggaagag gaggacaccg agcagcgtcc
     6421 gttcagcatc ccgggccacg cggggcatgc atccatcctt ccccgcccac ccacggatga
     6481 ctccggaccg agctcaggcg acgacctcga gggcctccgg caggccctcg agggtgggga
     6541 gctccgcggc ccgaggcagg tcgctccgct gcccgagccg attgatccag atcgcgggga
     6601 tccccagcct ggcgcacggc tcgaggtcgt ggaagggact cgccccgacg tggacgtgcg
     6661 actcccggtc gccggacagc gcgtcgaacc gttcccagtg ccccggagaa ggtttgtacg
     6721 acccggcgtc ggccgccgtg accgtcagtt cgaaggggac ggcgatgcgc tcgagcgagg
     6781 cggcgagcag atcggggtcg gtgttggaga ggatggccag acgccagccc cggtcccgga
     6841 ggtcccggag gacgcccggc acctcgggga agggcggcca ggtcggcagc gactcggcca
     6901 gcacgttctc ctctccggcc ggcagcccga ggaccgcgcc gacggccacc cgggcccggt
     6961 ccaggacctt ccggtacgga agcgtgggct cggcgtcctg gatcagcggc tcaaccgcgt
     7021 ggaagcccgc gaggagccgc cggcggtcgg cccggggcca gaggccggcc agcgcgtcgc
     7081 cgatgccccg gtcccagtcg atcagggttc cgtagcagtc gaaggtcgcc cagcgcgcca
     7141 cggggccaac cttacgggtg cgccttcggc gacctcaacc cgcccgggtc caggacgtcg
     7201 aggtgaagta ccgaggcctg aggtcgccga aggcgcacgc gtcctccacg agctcgaagg
     7261 tgagttcgtc accttcgatc gtccagcgat acacgccctc ggcggaggta cagttcgggt
     7321 cgttgatcag cgtcgcggta tcggcctcca cgaagacatg tcccgcgttc gtccacccgt
     7381 gaagtgtgtg ctcgatctcg aacgtccccc gaacgatcgt caaggtgttc gtgtccccca
     7441 gctcgagtcg gtacgggggg cagcgccgac acttccccac cggtccggcc agctcgtccg
     7501 ggacctccct gatccagacg ccgtccaggg gcgtcacctc cggcggcggg gtcggagtgg
     7561 tgaacggata gggatagggg acgcccgagg cggtcgcctc gggcgcgggc tcggcggggg
     7621 cctcgccgga gcatgcagcg gcgagcaggg cgaggatcac gatcactcgt cgcatgacgc
     7681 taatcaacca cgaccgggca gcccgcacca tgtcgccgag gtcgccgtca cgtccccgcg
     7741 tcatagagga caatgggaac gtgatcacgc gagtcttccg ggggacggtg cggccgggcc
     7801 agcacgcgga gttcgagcgg ctgttcaggg aggaggccgt gcctcggttc gagacgacgc
     7861 ccgggtgcct ctccgtccac ttcgggacgc ccacggaggc ctctcccgac gagtacatga
     7921 tcgtgaccgt ctgggaggat ctcgactcgc tcaagggttt cgccggcgag cagtggtcgg
     7981 agccgaagct cagcctccac gaggcgcacg tgctggccga cgccaccgtg caccactacc
     8041 ggaacgggga cggggagacg atcgtgccgc ccgcctcgct gcggggcgtc gggcgtcccg
     8101 ccgaggtgct cgacctgggg cggctccggg tcgacctggc ccggcgggtg gccgagctcg
     8161 acggcagggt ggtcgagctg ccgccccgtg agttctcggt gctttccgaa ctcgccctgc
     8221 gtgccggcga gcccgtgccc tccgacgagc tggcccgcct ggtgtggccc gaggacgcct
     8281 ggcccaccgg agaggacgtc cgccgctccg tgtaccggct ccgcaggctc ctcggcgacc
     8341 ggagccgggc tcggcccctg atccggaccc gtcggggcca cggctacgtc ctggaccccg
     8401 gcggcgatgg gacatcgccg ggacacggcc gccattcacg cacggccgcg cgtgcctgag
     8461 actccctccg cgatgagagc gaccgcggtg gccaccgtgc tcgtcctcgc ctcctgcgcg
     8521 aggctccccg acccgtccac gctccccggc gagtacgaga gcacgacggc cccggcgacc
     8581 ctgaccctga aggacggcga gtggaccctc gataacgggg ggttcgtgaa gtcgggcgcc
     8641 tacgggatct ccggcgatcg cgtggccttc ctgatcacgg agacgagtca tcccgcgttc
     8701 gacaactact gccgcgagga ggccgacgtg tacgaatggc gcttcgacga tgacctgctg
     8761 gtgctccggg cggtcgggga cacgtgcgac cccgtggggc agtcggtcct cacgcacgga
     8821 ccctgggagc gggcatgagc tgggaggtgc gcgcggctcg agtgatggcc tgggtgacgc
     8881 tcgtgacggg gggcggcctg gggatcgcct gcttcgttct ggccccggcg aaggtcacga
     8941 cgaccgagcg catcgtcggg cacgccacca tcacgtacga gggtgtcgag ctcgtcgttc
     9001 gtgagtcctt catcctcgcg gggatcgcct ccctcgtcct gggcgctgcg ctctgggcgc
     9061 tcctgctgct cgccgcccgc atcgcgagcg ctcgggacgg ctgagcgagc cgggaactca
     9121 cctccgccct ctcccgtcga acacccagac gacccgggag ggcgagatgc gagcgcttcg
     9181 tgcactggct gcgatgggga tcctgctggc gtcctgcacg tcgggaccgg tgaccggcct
     9241 ggggggcatc ccccagctcc tagccggagg gctccagccg ttcgacgact gtgaggactt
     9301 cctctcctac gtgaagggtc acggcctgga gcacgtgacc gcgtgggggc tgggcggagg
     9361 atggggtccc gtcttcttcg acgccgaggt cgcggcggag ggagaattcc gggcggcaca
     9421 gcccacggcg ggcgtcgact actcgaccac gaacatccag acctccgggg tggacgagcc
     9481 cgacatcgtt aagaccgacg gcgagcggct gctggccctg gtccgggggc ggctcttcgt
     9541 cttcgacgtc tcggggagcg ctcccaggaa ggtcggctcc ctccgtctcg gggacgtctg
     9601 gatgcgggac atggtgctgc acggcgaccg cgccctcctg ctcggagaag gctggggcgg
     9661 gggtccgggg ccggagcctc tcaggcgcga cgtcgtctcg cacgggggct cgatcagcct
     9721 cctcatggag gtcgatctca ccgatccgtc cgacccggag atcgttcggc gcctctacct
     9781 cgacggccgc tacctgtccg cgcggattgc cgacggcatc gtccgggtcg tggtcagctc
     9841 cttcccagtg ggcctggagt tcgagttccc cggggagcgg gggggtctgc aagcgaagcg
     9901 cgaggctctc gagcacaacc gtggtgtgat ccgagcgtcc acggtcgagg actgggtccc
     9961 gcaccacgtg ctggaggatg gcgacggaac ggtgctcgcg gagggacctc tcctcgactg
    10021 cgcctcggcc catcacccca ccgagttcgc gggcttcgac atgctgaccg ttctgacctt
    10081 ggacctcgag gagggcctga gcgcgtccgc gaccgaggga tccgtcggcg ttctgtccgc
    10141 aggtgagacg gtgtacgcct cgcccgagag cctctacgtg gccacgcagc gctggatcga
    10201 tccgatcgcg ttcgagcggg ggatgcccgc ggacctccgc gaggacttca ccacgcagat
    10261 ccaccgcttc gacatctcgg aagatgagcc cgcgacctac cggtcgtcgg ggcgggtccc
    10321 cggctggctc ctcaaccagt tctcgatgga cgagcacgag gggcggctcc gcgtggcctc
    10381 caccgactcc cccgagttct gggcaacgca aaaggccgag agcgtggtga ccgtgctcga
    10441 ggagcgcgca ggagccctgg tgccggtcgg gtccgtgggc gggctgggcg agggcgagcg
    10501 gatctacgcc gttcggttca tgggcgacgt cggctacgtg gtcacgttcc gccaggtgga
    10561 cccgctgtac gtcgtggacc tctcggatcc ggcgaccccc gcggtcaccg gggagctgaa
    10621 gatccccggc tactccgcgt acctgcaccc ggtgggcgag ggccgactgc tcggcgtcgg
    10681 gcaggacgcg accgaggggg gcctgacgcg agggacgcag ctctcgctgt tcgacgtctc
    10741 cgatccggcg gatcctcgcc gcatcgacac cgtaaagatc gcggacggca gctccgaggc
    10801 ggagtgggat caccacgcgt tcctctactg ggagcccgag ggcctcgtcg tggtcccgat
    10861 caccacgtgg ggagacgagc tgggaggcgg tgggttctcc ggggcggtgg ccgttcggct
    10921 ccagggcgac ggtctgtgga aggccgggac gctcagccac ggagacggcg cggtcggcga
    10981 atggttgccg ggtgggatcc gccgctcgct cgtggtcggc gacctcctct acacggtctc
    11041 ggagaccggc ctcgaggcgt ccgacctcca gacgctcgac gacgtggcct ggattccgtt
    11101 ccggtgagga gtggatcgca gagcgggacc cgcggccgga gctagtcggt cgcgagctcg
    11161 cgggccaggg agacggcctc gtcccagttc agggccttcc cctcgtccca ggcccgccgg
    11221 gccgcctcat cggggagggc gtccttcgcc tcctggaccg ggtctccgat caaggctccg
    11281 aggaagtcgg agggcggacc tcccccggcg gcgtctcgcg ccacctccca ggccccggcc
    11341 aggcggaccg catcctcgtg tcgtccctgc cacgagagga tgaaggcgag gtcgaggatc
    11401 acggaggcga tcccggtcgg gttccgcgcg gcagagaaca gctcgagagc ctcgtggatc
    11461 gcggtccggc tctcgtccca ccttccgacc cgcccgtaac cgaccccgag gttcgacagc
    11521 ccgttcgc
//
LOCUS       DASUTW010000022         2198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_915393, whole genome shotgun sequence.
ACCESSION   DASUTW010000022 DASUTW010000000
VERSION     DASUTW010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915393"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VF058_00860"
     CDS             complement(<1..466)
                     /locus_tag="VF058_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010470065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX6206886.1"
                     /translation="MSTNLGWRTFLVLVVLGGAIALILTRPVRLGLDLRGGTQIVLEA
                     RDTENVEVGGDVVDRTVEVLRRRVDALGVTEPTLQASGSRRIIIELPGVSDPDEAIRV
                     LGRTAQLTFHRVLGVEGPQAGPSPGSSPGVDEDGEEGQLVLPDESGGELRLGR"
     gene            complement(513..878)
                     /locus_tag="VF058_00865"
     CDS             complement(513..878)
                     /locus_tag="VF058_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206887.1"
                     /translation="MARRVLVRGLAAAFAAMLVPGTVWSEPAADRARAAEPPSSEATL
                     AQHPSPLGLVGGRSEDGWATRRDERTPTGDQMLGALLLGVAIALLAGTERVAARRTRR
                     SIPWTVRALGARSPPLVPA"
     gene            1170..>2198
                     /locus_tag="VF058_00870"
     CDS             1170..>2198
                     /locus_tag="VF058_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206888.1"
                     /translation="MKLATRLLFALLLPLMMIVASPVSASHSADDHSDNMREVAFSPR
                     SSPATQSDLAFVGKMAIAGNYNGFRIFDISDPENPTLVKDVFCRGQQGDVSIWGDALV
                     VSVDEVLTGPECGSGPALPATLETGWEGLRVFSLSEVLSTPADPDGFTRLQPAADIYI
                     KCGSHTHTGIPHKGHVDIYVASYPLRSGPDCGPHDDPSDTHNPLHEILSIARVDPQHP
                     EQSGGVNEVPIDIPTFNEDPQFLPPPAFNPMRGCHDIQVHLQLKLAAAACSSVGQVWD
                     ISDPHNPGTLDPVWEVDEPEVQFYHSALFSADKTTVIFGDEIIVGHCFDGTGSGQLWF
                     YDLATGDLQ"
BASE COUNT          341 a          824 c          714 g          319 t
ORIGIN      
        1 cccgcccgag cctcagctcc cctcccgact cgtccggcag gacgagctgg ccctcctctc
       61 cgtcctcatc cacgcccggc gatgagccgg gcgagggtcc cgcctgcggc ccttccacgc
      121 cgagcactcg gtggaacgtc agctgcgccg tgcggcccag gacgcggatg gcctcgtccg
      181 ggtcggatac gccggggagc tcgatgatga tgcgccggct tccggaggcc tggagggtgg
      241 gctcggtgac acccagggcg tccacgcggc gccgcaggac ctccacggtt cggtcgacca
      301 catccccgcc cacctcgacg ttctccgtgt ccctcgcctc gaggacgatc tgggtcccac
      361 cgcgaagatc gagcccgagc cgaaccggcc tcgtcaggat gagggcgatg gcccccccga
      421 gaacgacgag gacgagaaac gtgcgccatc cgagattcgt tgacatccag atcctccggc
      481 cggcgccgcc gtgcggcggc gcgttccacc agctaggcgg gcacgagcgg gggcgaccgc
      541 gcccccagcg cccggacggt ccacggtatc gagcgtcgcg ttcggcgtgc cgccacgcgc
      601 tcggttccgg cgagcagggc gatcgccaca cccaacagca gcgcgccgag catctgatcg
      661 ccggtcggtg ttcgctcgtc gcggcgcgtg gcccagccat cctcggatct gccgcccacc
      721 aggcccagcg gcgagggatg ttgcgccaag gtcgcctccg acgagggagg ttccgcggca
      781 cgcgcgcgat ccgccgcggg ctccgaccac accgtgccgg gcacgagcat ggcggcgaac
      841 gcggctgcca atccccggac gaggacccgg cgcgccatcc ccgctaccct accggaccgt
      901 cggggggccc gcggccgagt ctcggagtgg tctcgttcgg gggaaggttc cgcccgccgg
      961 cggcatctga gcccacggcg cggtctcctc cccgtcaggc acgcgggagc gtccccttcc
     1021 cacgcagacc gtccgtgatt tgaccctccg ctacagggac cagtaggttg ctcgggacac
     1081 cgcggaggcc cgaagggggt gatggatgac cgagcgatag cagaggctca tccttacccg
     1141 ccccacctgc cccaggaggt gacgatcccg tgaaactcgc aaccaggctc ctgttcgcgc
     1201 ttctcctccc cctgatgatg atcgtggcgt cccccgtgag cgcctcgcac agcgcggacg
     1261 atcacagcga caacatgagg gaggtcgcgt tcagcccgcg gtccagcccc gccacccagt
     1321 ccgacctcgc cttcgtcggg aagatggcga tcgcggggaa ctacaacggc ttccggatct
     1381 tcgacatctc cgatccggag aatcccacac tcgtcaagga cgtcttctgc cgagggcagc
     1441 agggcgacgt ctcgatctgg ggtgatgcgc tcgtcgtctc ggtggacgag gtcctgacgg
     1501 gccccgaatg cggatccggc cccgcccttc ccgcgacgct ggagacgggc tgggagggcc
     1561 tgcgggtctt cagcctcagc gaggtcctgt ccaccccggc ggaccccgac ggcttcactc
     1621 ggctccagcc cgcggccgac atctacatca agtgcgggtc gcacacccac acggggatcc
     1681 cgcacaaggg tcacgtggac atctacgtgg cgtcctaccc gctccgctcc ggtcccgact
     1741 gcggccccca cgacgacccg tccgacacgc acaacccgct gcacgagatc ctctcgatcg
     1801 cccgagtcga cccgcagcac ccggagcagt cgggcggtgt caacgaagtc ccgatcgaca
     1861 tccccacgtt caacgaggac cctcagttcc ttccgccgcc cgcgttcaac ccgatgcgcg
     1921 ggtgccacga catccaggtg cacctgcagc tgaagctcgc ggccgcggcg tgcagcagcg
     1981 tcgggcaggt ctgggacatc tcggatccgc acaaccccgg gaccctggac cccgtgtggg
     2041 aggtggacga gccggaggtg cagttctacc actcggccct gttcagcgcc gacaagacca
     2101 ccgtgatctt cggcgatgag atcatcgtgg gccactgctt cgatgggacg gggagcggcc
     2161 agctctggtt ctacgatctg gccacgggtg atctccag
//
LOCUS       DASUTW010000023         3400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_137619, whole genome shotgun sequence.
ACCESSION   DASUTW010000023 DASUTW010000000
VERSION     DASUTW010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137619"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VF058_00875"
     CDS             complement(<1..346)
                     /locus_tag="VF058_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX6206889.1"
                     /translation="MAKDEPKVQWRELYDEVVATGLCTGCSACVVVCPFHVLGYEDDK
                     PIQLEEGPANACTHGDNGCSICTRACPRFREWEPAIDELMFGRRRLPEEAIGVHREIL
                     LARAADPAVLASG"
     gene            complement(372..713)
                     /locus_tag="VF058_00880"
     CDS             complement(372..713)
                     /locus_tag="VF058_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX6206890.1"
                     /translation="METTRSVLSVTPQAAAKARSLAEREGRAQAWLRVRVVAGGCSGF
                     SYKLSFEDGAAEDDHVVEDHGLRVIVDPASIPILEGSTLEFRDALLGGGFKIENPRAQ
                     HECSCGESFSV"
     gene            741..1532
                     /locus_tag="VF058_00885"
     CDS             741..1532
                     /locus_tag="VF058_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6206891.1"
                     /translation="MELGLKDRPALVAAASRGLGRACAEVLAEEGARVAICARDAGAL
                     EATRDEIAEATGATVVAVPADVATERGAVGFVREGAEALGGCEILVPNVGGPRTGTFD
                     ELDDDDFANAFELLFLSSVRMAREALPHMRAAGYGRIVIIGSSAVKQPIPGLMLSTSI
                     RTGLIGWARTLVDEVGPEGITVNAVLPGRHATDRIRELHEDRARREGVSPAEAAAEDA
                     SQIPLRRIGDPRELAHVVAFLASERASYVSGAFVQVDGGMYRGLL"
     gene            1532..2764
                     /locus_tag="VF058_00890"
     CDS             1532..2764
                     /locus_tag="VF058_00890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6206892.1"
                     /translation="MLQVVDVETQPIRAYEPFIGAEGVEELVALGRELAGARVLHLNA
                     TAYGGGVSELLRSMIPIYRGLGIEAEWQVITGDEAFFEVTKCLHNALQGGKFPLGDRG
                     RATYVEQSRRNAEALEGEWDFLIVHDPQPVAMRHFRPGDGGRWIWRCHIDTSNPNSAA
                     VELLEPYVEEHDAIVFTLPQFAPEAWDNSVVRTMAPGIDPLSPKNMDLPPQVHDQIAL
                     WLGVDPHRPLMTQVSRFDSWKDPMGVVDVFRVVREEVPGLQLALLGSMALDDPEGWRL
                     YDRIREEAVGDRDLHVATNITGVSNIEVNAFQRLSDVVIQKSIREGFGLVVSETLWKE
                     TPVVAGRAGGIPVQMPEGIGGVLATTDEEYAEGVLRMLREPELARDLAAKGREHVRRH
                     FLITRLVADELRLLRDLG"
     gene            2929..>3400
                     /locus_tag="VF058_00895"
     CDS             2929..>3400
                     /locus_tag="VF058_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206893.1"
                     /translation="MASTVVSVLLLSGALFAAPGSAGTAGSNQARGFFVPPEIGQAVK
                     RTPGNLGGASDLFVEGELVVQFKKGVKRRAAMQVHAARGGARVLNGVTGLGGRIEHVK
                     LPAGVSVAQAVRRYQSDPNVAVAEPNFRYRWLSHITPPVNDTDILHLWGLGNTGQ"
BASE COUNT          489 a         1163 c         1252 g          496 t
ORIGIN      
        1 ggcccgaggc gaggacggcg gggtcggcgg cccgagccag gaggatctca cggtgcaccc
       61 cgatcgcctc ctcggggagc cgtctccgtc cgaacatcag ctcgtcgatc gcgggctccc
      121 attcccggaa ccgggggcag gcccgcgtgc agatcgagca gccgttgtcc ccgtgggtgc
      181 aggcgttcgc cggcccctcc tcgagctgga tcggcttgtc gtcctcgtac ccgaggacgt
      241 ggaaggggca gaccacgacg caggcggagc atccggtaca gaggccggtg gccacgacct
      301 cgtcgtagag ctcccgccac tggaccttgg gctcgtcctt ggccatatcg gccagtgtat
      361 aggcgggggc ctcagaccga gaacgactcg ccgcaggagc actcgtgctg ggccctcggg
      421 ttctcgatct tgaaccctcc gcccaggagc gcgtcgcgga actcgagcgt ggagccctcc
      481 aggatcggga tgctcgcggg gtccacgatc acgcggaggc cgtggtcctc cacgacgtgg
      541 tcgtcctcgg ccgcgccgtc ctcgaaggaa agcttgtagg agaaccccga gcatccgccg
      601 gccaccacgc ggacccggag ccaggcctgg gcgcgcccct cgcgctcggc gagcgaccgg
      661 gccttggccg cggcttgggg ggtgacgctc agcacgctgc gggtggtctc catctcgccc
      721 ctgatgatat caacggaacc gtggagctag gtctgaagga ccgtccggcg ctcgtggccg
      781 ccgccagcag ggggctgggc cgcgcgtgcg cggaggtcct ggccgaggag ggcgcccgcg
      841 tcgcgatctg cgctcgcgac gcgggcgccc tcgaggcgac ccgggacgag atcgccgagg
      901 ccaccggcgc cacggtcgtg gcggtgccgg ccgacgtggc cactgagcgg ggcgcggtgg
      961 gcttcgtccg ggagggggcg gaggccctgg ggggctgcga gatcctcgtc cccaacgtgg
     1021 gcggcccccg cacggggacc ttcgacgagc tcgacgacga cgacttcgcg aacgcgttcg
     1081 agctgctctt cctctccagc gtccggatgg cgcgggaggc cctcccgcac atgcgggccg
     1141 cgggctacgg gcggatcgtg atcatcgggt cgtcggccgt gaagcagccc atcccgggcc
     1201 tcatgctctc cacgtcgatc cggaccgggc tgatcggctg ggcccgcacg ctcgtggacg
     1261 aggtcggccc ggaggggatc accgtgaacg cggtcctgcc cgggcgccac gccaccgacc
     1321 gcatccgcga gctccacgag gatcgggccc ggcgcgaggg cgtctcaccc gccgaggccg
     1381 cggccgagga cgcctcccag atcccgctcc ggaggatcgg cgacccccgc gagctcgccc
     1441 acgtggtggc cttcctcgcc tccgaacgcg cctcgtacgt gtccggcgcg ttcgtgcagg
     1501 tggacggtgg gatgtaccgg gggctcctct gatgctccag gtcgtcgacg tcgagaccca
     1561 gccgatccga gcctacgagc cgttcatcgg ggcggagggc gtcgaggagc tggtcgccct
     1621 ggggcgggag ctcgccggcg ccagggtcct ccacctcaac gcgaccgcgt acggaggcgg
     1681 ggtctccgag ctgctgcgct ccatgatccc gatctaccgg ggcctgggga tcgaggcgga
     1741 gtggcaggtg atcacgggcg acgaggcgtt cttcgaggtc acgaagtgcc tccacaacgc
     1801 gctgcagggg ggcaagttcc cgctcggcga tcgggggcgg gcgacctacg tcgagcagag
     1861 ccgtcgcaac gcggaggccc tggagggcga gtgggacttt ctcatcgtgc acgaccccca
     1921 gcccgtggcg atgcgtcact tccgccccgg ggacggagga cggtggatct ggcgctgtca
     1981 catcgacacc tcgaacccga actcggccgc ggtcgagctg ctcgagccct acgtcgagga
     2041 gcacgacgcg atcgtcttca ccctcccgca gttcgccccg gaggcatggg acaactccgt
     2101 cgtccggacg atggcgccgg ggatcgaccc gctctcgccg aagaacatgg acctccctcc
     2161 gcaggtccac gatcagatcg cgctctggct gggcgtggac ccccacaggc ccctgatgac
     2221 ccaggtctca cggttcgatt cgtggaagga cccgatgggg gtcgtggacg tcttccgggt
     2281 cgtccgcgag gaggtgcccg ggctccagct cgcgctgctc gggtcgatgg ccctcgacga
     2341 ccccgagggc tggcgcctct acgatcggat ccgcgaggag gccgtggggg atcgcgacct
     2401 ccacgtggcc acgaacatca ccggggtcag caacatcgag gtcaacgcct tccagcgcct
     2461 ctccgacgtc gtgatccaga agtcgatccg cgaggggttc gggctcgtcg tctcggagac
     2521 gctgtggaag gagacgcccg tcgtggccgg acgcgccggc ggcatcccgg tgcagatgcc
     2581 cgaggggatc gggggcgtcc tggccacgac ggacgaggag tacgcggagg gggtcctccg
     2641 catgctccgc gagcctgagc ttgcgcggga cctggccgcg aagggccgcg agcacgtccg
     2701 ccggcacttc ctgatcacga ggctggtggc ggacgagctc aggctccttc gggacctcgg
     2761 gtagggtcct gacacgacca gagccttgtc cggtatatgg gggacggggg gcgcgagaat
     2821 gaccattgaa gagcttccga gaagcgcgta tcgtggcccg cgccgccgag gtgtctccgg
     2881 gcggcgcttg gcgcccgcag aagagaggga ggattcttga agcgttctgt ggcctcgact
     2941 gtcgtatccg ttctcctact gtccggggcg ctgttcgccg caccggggtc ggcgggcacc
     3001 gcgggctcca accaggctcg gggcttcttc gtgccgccgg agatcgggca ggcggtgaag
     3061 cgcacccccg gaaacctcgg cggcgcctcc gacctcttcg tcgagggcga gctggtcgtg
     3121 cagttcaaga agggcgtgaa gcgacgggcc gcgatgcagg tccacgccgc acgcggcggc
     3181 gcccgggtcc tgaacggcgt cacgggcctg ggcggccgga tcgagcacgt gaagctaccg
     3241 gccggcgtct cggtggctca ggcggtgcgc cgctaccaga gtgacccgaa cgtcgcggtg
     3301 gccgagccga acttccggta ccggtggctg agtcacatca cgccgccggt gaacgacacc
     3361 gacatcctgc atctgtgggg gttgggcaac acaggccagg
//
LOCUS       DASUTW010000024         9116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_785255, whole genome shotgun sequence.
ACCESSION   DASUTW010000024 DASUTW010000000
VERSION     DASUTW010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9116
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785255"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VF058_00900"
     CDS             <1..400
                     /locus_tag="VF058_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX6206894.1"
                     /translation="HPVIDLLPEQQDVTDMGGTMRLGAQPCHIAEGTLAERAYGEPVI
                     YERHRHRYEVNPKYHRQLTEGGMALSGMSPDGRLVEIIELPGHPFFIAGQFHPELKSR
                     PDRPHPLFREFVGAAKAYRQAPQPAAAGAE"
     gene            397..891
                     /locus_tag="VF058_00905"
     CDS             397..891
                     /locus_tag="VF058_00905"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX6206895.1"
                     /translation="MTSPEIERVYEGPIFSVEILRWPGKRRDVVRALGASAVLPLTSD
                     GHVILVRQEREAVGERLLEIPAGLLDVEGEPPLETARRELREETGHRAGSIEPLGRLH
                     TSPGFVDEVCHLFLGTGCTPEGDPEEGMEVVALPLAEAVAMVGRGEITDSKTVAALLL
                     AARR"
     gene            complement(888..1544)
                     /locus_tag="VF058_00910"
     CDS             complement(888..1544)
                     /locus_tag="VF058_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006835914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX6206896.1"
                     /translation="MAELKALFLNCTLKRSPEVSNTEALIRKVIEWFDRLDVESEIVR
                     VVDYHVPFGVTSNEGEGDEWPIILDKVKAADILVIGTPIWFGVRGSVAQMVIERLDGT
                     YSERNEVGQYPLYNKVGGVVVTGNEDGAHAAAETTLFNLSHLGCTIPPNADTYWVGDA
                     GPGPSYIEAGGEHHAYTKRTTRWMAHNLVHLARMLKANPIPADGNTFEEEDEHAPHVG
                     "
     gene            1610..2551
                     /gene="xerD"
                     /locus_tag="VF058_00915"
     CDS             1610..2551
                     /gene="xerD"
                     /locus_tag="VF058_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017591282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX6206897.1"
                     /translation="MSDAHVRRFLDHLAVERGLSSNTLEAYRRDLRRYLGFLGERGVS
                     QATRASEEDVAAFVRHLSGVEYAPERRYRASSVARALAAVRALHRFLAREGLAEDDPT
                     QGVIRPRIPRSLPKPLSVDEVAALLAAPGDAEPPALRDTAILEVLYGAGLRISELVAL
                     DVDDVDLEEGSVRVVGKGDKERVVPLGRYAVRAVEGYLARGRPALAGARTRGALFLNQ
                     RGGRLTRQGATNIIQSAARRAGIRARVTPHVLRHSFATHLLEGGADVRVVQELLGHAS
                     VATTQIYTLVTEDRLREVYDEAHPRAKRTAAAGRGSG"
     gene            2548..3723
                     /locus_tag="VF058_00920"
     CDS             2548..3723
                     /locus_tag="VF058_00920"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEX6206898.1"
                     /translation="MRVLVLVGDSWGVGAAPDAAAYGDEGSDTLGNASRAAGGIRAPN
                     LGHLGLGMLTEIEGVPPEAGPGTFHGKMAEHSAGKDSTTGHWEMMGIVLDRPFPTYPE
                     GFPSEVMEPFEEAIGMKALGNVPASGTVIIEELGEEHLATGRPIVYTSADSVFQIAAH
                     KDVVPLERLYEWCRIARGLLYGDHLVGRVIARPFVGELGSFTRTHERRDYSVEPPSET
                     YLERIQAAGVPVYGVGKIPDMFVGRGLTESRHSESNANGVDLTIEYLSRGDHMLVFTN
                     LVDFDSKYGHRNDPQGYARCVEELDARLPELLEAVADDGILFITGDHGCDPTDPSTDH
                     TREFVPVLGGGGALVDRSVDVGTRETFADLGATVAALFGVAPDGLSGTSFTSELGLR"
     gene            3720..5033
                     /locus_tag="VF058_00925"
     CDS             3720..5033
                     /locus_tag="VF058_00925"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEX6206899.1"
                     /translation="MRRDPRDVVAAKRDGRKLPEEDMREFVLAYGRGEIDDALAAAFL
                     MASFINGLDTDETVAMTRAMVESGETLPLEGVSRPKVDKHSTGGVADSVTLIFAPLAA
                     SLGLAVAKLSGRGLGHTGGTLDKLESIPGLRTDLSPREMERQVEEVGCAVAAQTKDLV
                     PADGAFYALRDATATVPSTPLIAASIMSKKLAVGTDLILLDVKTGSGAQMKRLTEARD
                     VASACSALAEEWGRRARVAVTDMSQPLGDAVGNALDIAEAIRALRGEEEGRLMELVVE
                     FASAALVSLQDVPEDEGRDRARTALRDGSALEAFRRMVEAQGGDPRVVDDPETVLPGA
                     PVIEPLMSTREGWLGEVDAEEVGKASVMLGAGRLKKGDPIDPAVGVVFRTKIGDRVER
                     GEPMGEVHARDEEAAAEAADRVRAALTFSEDEVEAPPLLYGWHGA"
     gene            5030..5677
                     /locus_tag="VF058_00930"
     CDS             5030..5677
                     /locus_tag="VF058_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX6206900.1"
                     /translation="MSVESILEVAYFIVALIPAVVFHEVSHGLVADRLGDPTARAAGR
                     LSLNPIRHIDPFGTVILPGLLLLPRLFGQVGAPVFGYAKPVPINPQNLREPDRQMMWI
                     AAVGPLTNLFLALVGALAFRLVGVGGGELAQFLFVFILVNVILAVFNLLPLPPLDGSK
                     VLARFLPPRAREVYRSLEAYGVLILLVIFFIFPQAIFGFITPIVRGLIELFTGLG"
     gene            5718..6476
                     /locus_tag="VF058_00935"
     CDS             5718..6476
                     /locus_tag="VF058_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX6206901.1"
                     /translation="MTAMEEDRALARVGEFPIELPVFTGPFRVLAELLLDRKVDVCDV
                     SIAKVVEEFVGRADEAEGWSLEEATWFLAIGAVLLELKVGRLLPKREKPVDEEDLVGG
                     SPDLAYARRLELAAFRKVAQDLALRVEASARHAAREAGPPRELAHLYPDVLEGVEPTA
                     LAETMAALLRPAPTIDLSHVTPIRLTVEDGVEAVVGHLSGRGDARFRDLVADCRDRID
                     VVVRFLALLELYRDGRVRLRQAETFGDIEVSWEG"
     gene            6476..7045
                     /gene="scpB"
                     /locus_tag="VF058_00940"
     CDS             6476..7045
                     /gene="scpB"
                     /locus_tag="VF058_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX6206902.1"
                     /translation="MDPRLRDLEAVLFVADEPLTAAALGQVLDASRAEVDGLLTDLQG
                     ALEERGSGLTLRNVAGGWRLYTSAEALPAVERFVRSSRHTRFTKASLETLAIVAYKQP
                     VTRHQVSAIRGVDSDGVLRALTDRGLVAEVGREDSPGRPVLYGTTPEFLERLGLASVS
                     DLPSLAPLLGEAEEEEDDGAAPGADDPAA"
     gene            7005..7760
                     /locus_tag="VF058_00945"
     CDS             7005..7760
                     /locus_tag="VF058_00945"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX6206903.1"
                     /translation="MTARPRERTTPPPEPERLQKVLARAGIGSRRASEALIRDGRVTV
                     NGRVATLGDRVDPSRDVVEVDGGRVPLDPDLRYYALHKPAGVVTTARDPQGRPTVADL
                     YPEGPRVFPVGRLDRETEGLLLLTNDGELANRLTHPRYGVEKEYLAEVEGDPSPEAIR
                     RLMRGVELEDGVAKAASASRVAGSRGRAALRLVMTEGRKREVRRMLASVGLPVARLVR
                     TRVGPIRLERLSPGEARKLEPPEVAELYRASGG"
     gene            7760..8116
                     /gene="aroH"
                     /locus_tag="VF058_00950"
     CDS             7760..8116
                     /gene="aroH"
                     /locus_tag="VF058_00950"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018570457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX6206904.1"
                     /translation="MRLRGIRGAVSVEADTEEEILTATGGLLREILDRNELERDDVVS
                     IVFTATPDLRSAFPAAAARAIGLDVPVLSSSEMDVEGALPRVVRVLLHGYTERSQDEV
                     VHVYLGKAVSLREDLA"
     gene            8113..>9116
                     /locus_tag="VF058_00955"
     CDS             8113..>9116
                     /locus_tag="VF058_00955"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX6206905.1"
                     /translation="MRVAILGTGLIGASIGLAARRWLDAEVAGHDPDPLALEAAARRG
                     AVEPAESAAGAAAAADLVFVCAPVAAISEAAAAALEAGARVVTDAGSVKAGIVAAVEG
                     LGGGGAGRFVGGHPMTGSERSGPAGAAAGLIDGAAWALTPTDRTDPAALASVEDAVRR
                     MGAVPVTLSPDRHDRLVGLVSHLPQVASIALMSTVASSADETALLLAAGGFRDLTRLA
                     ASSPELWVDILLANREAVAEALDGYGERLRSLAGMLQAGDAEGLTETFARAKEARLGL
                     GARPQSRVGLAILAVPIPDRPGSLAEIAGALADRSVNIEDLEIVHSAEGARGTAHVTV
                     G"
BASE COUNT         1204 a         3120 c         3483 g         1309 t
ORIGIN      
        1 gcacccggtc atcgatctcc tgcccgagca gcaggacgtc accgatatgg gcgggacgat
       61 gcggctcggc gcgcagccgt gccacatcgc cgaggggacg ctcgcggagc gggcctacgg
      121 cgagccggtg atctacgagc ggcaccgcca ccgttacgag gtcaacccca agtaccaccg
      181 ccagctcacc gagggcggga tggcgctgtc ggggatgtcg cccgacgggc gcctcgtcga
      241 gatcatcgag ctgccgggcc atccgttctt catcgccggc cagttccacc ccgagctgaa
      301 gtcgcggccc gaccgccccc acccgctctt ccgcgagttc gtgggggcgg ccaaggcgta
      361 tcgtcaggcg ccgcagccgg ccgcggccgg cgccgagtga cctcgcccga gatcgagcgc
      421 gtctacgagg gcccgatctt ctccgtcgag atcctgcgct ggcccggcaa gcgccgagac
      481 gtggtccgcg cgctgggggc gagcgcggtc ctcccgctga cctcggacgg ccacgtgatc
      541 ctcgtccgcc aggagcgcga ggcggtgggc gagcggctgc tggagatccc cgcggggctc
      601 ctcgacgtgg agggcgagcc gccgctcgag accgcacgcc gcgagctccg cgaggagacg
      661 ggccaccgcg ccggatcgat cgagccgctg ggacgtctgc acacctcgcc cggattcgtg
      721 gacgaggtgt gccatctctt cctcggtacc ggctgcacgc cggagggcga tcccgaggag
      781 gggatggagg tcgtcgccct acccctggcc gaggcggtgg cgatggtcgg gcggggggag
      841 atcaccgaca gcaagaccgt cgccgcgctg ctgctcgccg ctcgccgcta gcccacgtgg
      901 ggggcgtgct cgtcctcctc ctcgaacgtg ttgccgtcgg ccgggatggg gttggctttc
      961 agcatccggg ccaggtgcac gaggttgtgg gccatccagc gagtggtccg cttcgtgtag
     1021 gcgtggtgct cgccccccgc ctcgatgtag gaggggcccg gcccggcgtc gcccacccag
     1081 taggtgtctg cgttgggcgg gatcgtgcac cccaggtgcg agaggttgaa gagcgtggtc
     1141 tcggcggcgg cgtgcgcccc gtcctcgttc ccggtgacca ccacgccgcc caccttgttg
     1201 tacagggggt actgaccgac ctcgttgcgc tcgctgtacg tcccgtcgag ccgctcgatc
     1261 accatctgag ccacgctgcc ccggaccccg aaccagatcg gcgtcccgat cacgaggatg
     1321 tcggccgcct tcaccttgtc caggatgatc ggccactcgt cgccttcgcc ctcgttcgag
     1381 gtcaccccga agggcacgtg gtagtcgacc acgcgcacga tctcggactc gacgtccagg
     1441 cgatcgaacc actcgatcac cttgcggatc agcgcctcgg tgttcgagac ctcgggcgag
     1501 cgcttcagcg tgcagttcag gaagagcgcc ttcagctcgg ccatctcatc ctccctctgc
     1561 tcggagcggg gctcattctg gcacgaaggg ctccggtatc ctttcggcgg tgagcgacgc
     1621 ccacgtccga cggttcctcg accacctcgc cgtggagcgg gggctgtcca gcaacacgct
     1681 ggaggcctac cggcgggacc tccgccgata cctcggcttc ctgggggagc ggggagtgtc
     1741 ccaggccacc cgggcgagcg aggaggacgt cgcggcgttc gtgcggcacc tctcgggggt
     1801 ggagtacgcg ccggagcgcc gctaccgggc ctcctcggtg gccagggcgc tggcggccgt
     1861 ccgggccctg caccgcttcc tggcccggga ggggctggcc gaggacgatc cgacccaggg
     1921 cgtgatccgg ccccggatac cccggagcct ccccaagccg ctctcggtgg acgaggtggc
     1981 ggccctgctg gccgccccgg gcgacgcgga gcctccggcc ctccgcgaca ccgcgatcct
     2041 cgaggtgctc tacggcgcgg ggctccggat ctcggagctc gtggcgctcg acgtggacga
     2101 cgtggacctg gaggaaggga gcgtgcgggt ggtgggcaag ggcgacaagg agcgcgtggt
     2161 gccgctcggg cgctacgccg tccgggccgt cgagggatac ctcgctcgag ggcggccggc
     2221 cctggccgga gcccgcacgc ggggcgccct cttcctgaac cagcgcggcg gaaggctgac
     2281 ccgccagggc gccacgaaca tcatccagtc cgccgcccgc cgagccggga tccgagcgag
     2341 ggtcacgccg cacgtcctgc ggcactcgtt cgccacgcac ctgctcgaag gcggggccga
     2401 cgtgcgcgtg gtgcaggagc tgctggggca cgccagcgtg gccacgaccc agatctacac
     2461 gctcgtgacg gaggaccggc tccgcgaggt ctacgacgag gcccacccga gggcgaagcg
     2521 gaccgcggcg gccggccggg ggtcgggatg agggtcctcg tcctcgtggg ggattcgtgg
     2581 ggggtcggcg ccgctcccga tgccgccgcg tacggggacg aggggtccga caccctgggc
     2641 aacgcgtcgc gggcggcggg cgggatccgc gccccgaacc tcggccacct gggcctgggg
     2701 atgctcaccg agatcgaggg cgtgccgccg gaggccggcc cggggacgtt ccacgggaag
     2761 atggccgagc actcggcggg gaaggactcc acgaccgggc actgggagat gatggggatc
     2821 gtcctcgatc gtccgttccc cacgtatccg gagggcttcc catcggaggt gatggagccg
     2881 ttcgaggagg cgatcgggat gaaggcgctc ggcaacgtcc cggcctcggg cacggtgatc
     2941 atcgaggagc tgggggagga gcatctcgcc accggtcggc ccatcgtcta cacgagcgcc
     3001 gactccgtct tccagatcgc ggcccacaag gacgtcgtcc cgctggagcg gctgtacgag
     3061 tggtgccgga tcgcgcgggg gctgctgtac ggcgaccacc tcgtgggccg cgtgatcgct
     3121 cggcccttcg tgggcgagct cgggagcttc accaggaccc acgagcgccg cgactactcg
     3181 gtcgagccgc cgtccgagac gtacctggag cggatccagg ccgcgggggt ccccgtctac
     3241 ggggtgggca agatccccga catgttcgtg ggtagggggc tgaccgagtc ccgacactcc
     3301 gagtcgaacg cgaacggggt cgacctgacg atcgagtacc tgtcgcgggg cgaccacatg
     3361 ctcgtcttca cgaacctcgt ggacttcgac tcgaagtacg gacaccgaaa cgacccccag
     3421 gggtacgcgc gatgcgtcga ggagctcgac gcccgcctgc ccgagctgct cgaggccgtg
     3481 gccgacgacg ggatcctctt catcacgggg gaccacggct gcgatcccac cgacccgtcc
     3541 acggaccaca cgcgggagtt cgtcccggtg ctggggggtg ggggcgccct ggtggaccgc
     3601 tcggtcgacg tcggcacacg ggagacgttc gccgacctcg gcgcgacggt cgccgctctc
     3661 ttcggggtgg ccccggacgg tctttcgggg acgagcttca cctcggagct gggcctgcga
     3721 tgaggcgaga ccctcgcgac gtcgtggcgg ccaagcgcga cggccggaag ctgcccgagg
     3781 aggacatgcg cgagttcgtc ctcgcgtacg ggcggggcga gatcgatgat gcgctcgcgg
     3841 ccgcgttcct catggcttcg ttcatcaacg ggctcgacac cgacgagacc gtggcgatga
     3901 cgcgggccat ggtcgagtcg ggcgagaccc ttccgttgga gggggtctcg cgacccaagg
     3961 tcgacaagca ctccacgggg ggcgtggctg actctgtgac cctgatcttc gcgcccctcg
     4021 ccgcgtcgct cggcctcgcg gtggccaagc tgtcgggccg gggcctcggc cacacggggg
     4081 gcacgctgga caagctggag tcgatcccgg gccttcgaac cgacctctcg ccgagggaga
     4141 tggagcggca ggtcgaggag gtggggtgcg cggtggccgc tcagacgaag gatctcgtcc
     4201 ccgccgacgg ggccttctac gccctccgcg acgccacggc gaccgtccct tcgaccccgc
     4261 tgatcgcggc cagcatcatg agcaagaagc tggccgtagg gaccgacctt atcctcctcg
     4321 acgtgaagac ggggagcgga gctcagatga agaggctcac ggaagcaagg gatgtcgcct
     4381 cggcatgctc cgctctcgcc gaggagtggg ggcgtcgcgc tcgcgtggcc gtcaccgaca
     4441 tgtcccagcc gctgggggac gccgtgggga acgcgctcga catcgccgag gcgatccggg
     4501 cgctgcgggg cgaggaggag gggcggctca tggaactcgt ggtcgagttc gcctcggccg
     4561 ccctcgtatc gctccaggac gtgccggagg acgagggccg ggaccgggcc cggacggcgc
     4621 tccgcgacgg gtcggccctg gaggcgttcc ggaggatggt cgaggcgcag ggcggcgacc
     4681 ctcgggtggt ggatgacccg gagaccgtct tacccggagc tcctgtgatc gagccgctga
     4741 tgtccacgag agaggggtgg ctgggggagg tcgatgccga ggaggtcggg aaggcatcgg
     4801 tgatgctcgg tgccggccgc ctgaagaagg gcgacccgat cgaccccgcg gtcggcgtcg
     4861 tcttccgcac gaagatcggc gaccgcgtgg agcgcggcga gccgatgggg gaggtccacg
     4921 ctcgggacga ggaagcggcg gcagaagccg ccgaccgcgt ccgcgccgcg ctgaccttca
     4981 gcgaggacga ggtcgaggcg ccccccctcc tgtacgggtg gcacggcgca tgagcgtcga
     5041 gtcgatcctg gaagtcgcct acttcatcgt ggcgctgatc ccagccgtcg tgttccacga
     5101 ggtcagccac gggctggtgg cggaccgcct cggggatccc accgcccggg ccgcggggcg
     5161 cctctccctc aatccgatca ggcacatcga cccgttcggg accgtgatcc tccccggcct
     5221 cctgctgctg ccccggctgt tcgggcaggt cggcgccccc gtgttcggct acgccaagcc
     5281 ggtgccgatc aacccgcaga atctccggga gcccgatcgt cagatgatgt ggatcgcggc
     5341 cgtgggcccg ctcacgaacc tgttcctcgc gctcgtcggg gcgctggcct tccgcctggt
     5401 cggcgtgggg ggcggcgagc tggcccagtt cctcttcgtc ttcatcctgg tgaacgtgat
     5461 cttggcggtg ttcaacctgc tgcctcttcc gccgctggac ggctcgaagg tgctggcccg
     5521 cttcctcccg cctcgcgccc gcgaggtgta ccggtcgctc gaggcttacg gcgtgctcat
     5581 cctcctcgtg atcttcttca tcttcccgca ggcgatcttc gggttcatca ccccgatcgt
     5641 gcgggggctg atcgagctct tcaccggcct cggatgattg aggggctcct cggccgcggc
     5701 tacgatggcc ccgacccatg accgcgatgg aggaggaccg cgcgctcgcc cgggttggcg
     5761 agttcccgat cgagctgccc gtcttcacgg gtccgttccg cgtgctcgcc gagctgctcc
     5821 tcgaccgcaa ggtggacgtc tgcgacgttt ccatcgcgaa ggtcgtggag gagtttgtag
     5881 ggcgggccga cgaggccgag ggctggtcgc tggaggaggc gacctggttc ctggcgatcg
     5941 gcgcggtcct cctcgagctg aaggtgggtc ggctcctccc caagcgcgag aagcccgtcg
     6001 acgaggagga tctcgtcggc ggctcgcccg acctggccta cgcgaggcgg ctggagctcg
     6061 cggcgttccg caaggtggca caggacctcg cgctccgggt cgaggcctcg gcccggcacg
     6121 cggcccgcga ggccggcccg ccgcgcgagc tggcgcacct gtaccccgac gtgctggagg
     6181 gcgtggagcc cacggcgctg gccgagacga tggcggcgct gctgcggccg gccccgacga
     6241 tcgacctgtc gcacgtcacc ccgatccggc tgaccgtcga ggacggggtc gaggctgtgg
     6301 tggggcacct ctcgggccgg ggcgacgcgc gcttccgcga cctcgtggcc gactgccgcg
     6361 accggatcga cgtcgtggtg cgcttcctgg ccctgctcga gctgtaccgc gacgggaggg
     6421 tgcggctgcg gcaggccgag accttcgggg acatcgaggt gagctgggag ggctgatgga
     6481 tccacggctg cgcgatctcg aggcggtgct gttcgtggcg gacgagccgc tcacggcggc
     6541 ggctctgggg caggtgctgg acgcgtctcg agccgaggtg gacggcctcc tgaccgacct
     6601 ccagggcgcg ctggaggagc gggggtcggg gctcaccctc cggaacgtgg ccggcggctg
     6661 gcggctgtac acgagtgcgg aggcgctccc ggccgtggag cggttcgtcc gctcctcgag
     6721 gcacacgagg ttcacgaagg cctcgctgga gacgctggcg atcgtggcgt acaagcagcc
     6781 ggtgacccgc caccaggtgt ccgcgatccg gggcgtcgac tccgacggcg tcctccgggc
     6841 gctgaccgac cgcgggctgg tggcggaggt ggggcgcgag gacagcccgg gccgccccgt
     6901 gctgtacggg acgacgccgg agttcctgga gcgtctgggg ctcgcttccg tgagcgatct
     6961 gccgtccctg gcgccgctgc tcggcgaggc cgaggaggag gaggatgacg gcgcggcccc
     7021 gggagcggac gaccccgccg cctgagccgg agcggctcca gaaggtgctg gctcgcgcgg
     7081 ggatcgggtc caggcgcgcc tccgaagccc tgatccggga cggtcgcgtc accgtgaacg
     7141 ggagggtggc cacgctcggc gaccgcgtgg acccctcgag ggacgtcgtg gaggtggacg
     7201 gcgggcgggt gccgctcgat ccggacctcc gctactacgc cctgcacaag ccggccggcg
     7261 tcgtcaccac ggccagggac ccgcagggcc ggcccacggt cgccgatctc taccctgagg
     7321 gacctcgcgt cttccccgtg gggaggctcg atcgggagac cgaggggctg ctcctgctga
     7381 cgaacgacgg cgagctggcc aaccgcctca cccatcctcg ttacggggtg gagaaggagt
     7441 acctggccga ggtcgagggc gatccgagtc ccgaggcgat ccgccggctg atgcggggcg
     7501 tcgagctgga ggacggggtg gcgaaggcgg cgtccgcgag ccgggtggcg gggtccaggg
     7561 ggcgcgcggc gctgcggctc gtgatgacgg aggggcggaa gcgagaggtg cgcaggatgc
     7621 tggcgagcgt ggggctgccc gtggcgaggc tcgtccggac cagggtgggg ccgatccggc
     7681 tggagcggct gagccccggc gaggcgcgaa agcttgagcc accggaggtc gccgagctct
     7741 accgggcgag cggcggatga tgcgcctccg agggatccgg ggggccgtgt cggtggaggc
     7801 ggacaccgag gaggagatcc tgacggccac aggggggctg ttgcgcgaga tcctggatcg
     7861 gaacgagctt gagcgcgacg acgtcgtgag catcgtgttc acggccaccc cggacctccg
     7921 gtcggccttc ccggcggcgg cggcgcgggc gatcgggctc gatgtgcctg tcctgtcgtc
     7981 gagcgagatg gacgtggagg gggcgcttcc gagggtggtc agggtgctgc tccacggcta
     8041 cacggagcgg agccaggacg aggtcgtgca cgtgtacctg ggcaaggccg tctcgctccg
     8101 ggaggacctg gcgtgagggt cgcgatcctg gggacggggc tgatcggggc ctcgatcggg
     8161 ctcgccgcgc ggcgttggct ggacgccgag gtcgcggggc acgacccgga ccccctcgcg
     8221 ctggaggcgg ccgcccggag gggcgccgtc gagccggcgg agagcgcggc gggggcggcc
     8281 gcggccgccg acctcgtctt cgtctgcgct cccgtggccg cgatctcgga ggcggccgcg
     8341 gccgccctgg aggccggggc gcgcgtcgtc accgacgccg gcagcgtcaa ggccggcatc
     8401 gtggccgcgg tggaggggct ggggggcggc ggcgccggtc ggttcgtggg cggccacccg
     8461 atgaccggct cggagcggtc ggggccggcc ggcgccgccg ccggactgat cgacggcgcc
     8521 gcctgggccc tcacgccgac ggaccggacg gatcccgcgg ccctcgcctc cgtggaggac
     8581 gccgtccgcc ggatgggggc cgtccccgtg acgctcagcc ccgatcgtca cgatcgtctc
     8641 gtcggcctcg tcagccacct tccccaggtg gcctcgatcg cgctgatgtc cacggtggct
     8701 tcctcggccg acgagaccgc cctcctgctc gccgccgggg gcttccgcga cctcacccgc
     8761 ctggcggcca gcagcccgga gctgtgggtg gacatcctcc tcgccaaccg cgaggcggtg
     8821 gccgaggcgc tcgacggcta cggggagcgc ctgcggagcc tggcgggcat gctgcaggcg
     8881 ggcgacgccg aggggctcac ggaaacgttc gcccgagcca aggaggcccg tctgggcctc
     8941 ggggcgcgcc cccagtcccg ggtggggctg gcgatcctgg ccgtccccat cccggaccgg
     9001 ccgggctcac tggccgagat cgccggcgcg ctcgccgatc ggagcgtcaa catcgaggac
     9061 ctcgagatcg tgcacagcgc cgagggcgcc cggggcacgg cgcacgtcac cgtggg
//
LOCUS       DASUTW010000025         9850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1433749, whole genome shotgun sequence.
ACCESSION   DASUTW010000025 DASUTW010000000
VERSION     DASUTW010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9850
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433749"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..6699)
                     /locus_tag="VF058_00960"
     CDS             complement(<1..6699)
                     /locus_tag="VF058_00960"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX6206906.1"
                     /translation="MSATVLLLGLALLITSLAPASQAQASHGLLVSSSPDRSAPGALD
                     GAVVGGTIYVFVDPESGIKQVRFWLDNTAMSGKPRTTEKRAPWDFEGTVTSDGTAKPF
                     DSTAVADGPHTISAAIDLSAGGTTVVHASFTVQNADAQLSFTPASVDLAVQSGGTDST
                     SVELASSGPDPGSFQVSADAPWLSASPSSGTYPATVTVSVDASGLADGAYSGRVTATA
                     DGHTPAVLPVTLTVGDLGPADQIHLAWVDDPSTTMTVVWRTLAPDASSVVEYREQGSS
                     TWLQATGGTRTSGTTGTLREATLTGLVPATTYEYRVDGGSEGWSTVHTTRTAPAPGPA
                     DFSAIYFADTGLIGREDGLATGTEQAIQEMTALDPTLFLPGGDYIYYNTDTRYGSLDD
                     SIDAWFAQNEPFATRAPMMPTYGNHEVLLGESYSAWASRFPTPQGFDGRRFYSFDVGD
                     VHFVSILAVAETQGLSSAALDWIRQDIEAAKAAGQRWIVPYMHVPPFSDGKNHSSNLN
                     LRGQLGPLFEQLGVQVVLTSHDQSYERTYPLTDVPATNTPTSSSLSCYTMADGVTYVK
                     VSPAGKLSNKNGDFSQFATNPPPHWTAFRDNTLHHFSRLEVSSQGVLRVETYGFTGDG
                     TPPTMIDAFEYRDGACPGELKTSPGALNLSAGEGETVTAAVDLEVSDGSTASFDVATD
                     APWLTVSPLSGSTPATLSVEADASSLAPGTHQATLTATASGYDPVSVPVSLSVSSAGT
                     GYQLFRSGSPTRSNPTVLEGTTLQGDAYVFTGPDDGVSRVVFWLDDTSMTTTPFKSES
                     RAPFDFNGTAADDTAFPWDTTTVADGSHTITARLDLSSGTSEVVHATFTVSNHGPQLR
                     FQPGTLSLSGSEGDAPSATVDLSVTDGSAASYSLGTDKPWLTAEPASGTTPQAITVTA
                     DTTGLALGTYNGTVTATASGLDPVSLPVALTVGLADTGYRLLVSPSPDRSGFADLEGQ
                     TLQGNAYVFTGPDDGVKKVSFWLDNTAMTGTPTKVENGAPYDFAGTAKNGTALPWDTT
                     AVNDGSHTISALIELSAGGSDTVHASFAVANDAPQLRFEPTSLSLSGEQGDAPNATVQ
                     LEVTDGTQTAYDVTSDRPWLTAAPASGTTPSAITVTADTTGLAAGTYNGTVTASTAGF
                     GSVVLPVTLTVLAPGTGYRLLSSTTADRNTFADLEGQTLQGNAYVFTGPDDGVSKVSF
                     WLDDPQMTGTPTKVENGAPYDFAGTAQDGTAFPWDTTTVPDGQHTITARLDLSAGGSQ
                     VITSTFTIANNAPALAFTTDPLLFDVLEEGGSADPQTSGLITTDGTAADFIVDDDADW
                     LTVTPVSGTTPATLTFEVDTTGLPEGNYQAAATATAPGYDPATLLVTLAIGTGGCTPL
                     PCEEIKVSLPYRLDWGVDHGKILAGNGVGTGFTYVDPGGNGYLPEKLSVDTAQGVFQI
                     ATTSGGAAGTENGQDNALGVGIAAPNQAFIIETDVVNPPAGTGKFEQAGLWFGNDQDN
                     YVKIITLSTTNGTVIEYLIEVDGARPVSPKRTGVLDLSSSTATLILRANPFTRKIEGS
                     YRVDGGEEKVVASFTAPPEFFSFDAAGIDPRIGTRSFGGVFATHRGGSPLMYTFDNFS
                     VVEDTSVANPTTPEGFSFDKSSFPVPNPTSMVWGPDGRLYVTELFGKIHAVTLDANQQ
                     VVSDEVITTLGSRLTLGITVDPDSTASNVVLWVSHSSPSVNNGEVNSSTVTRLSGSGF
                     TAREDVITGLPRAKANHAINSIHFGPDGRLYMAHGGNTGAGAPNLANTEFGDRPEQPL
                     SAALLVADVKATGFEGSCATPLGEFGVPATCDVSVYASGLRNTYDFVHHSNGSIYAPD
                     NGLGVTGTYPPKPTPDCTGLADASQHNPGEQPDIINRLEQGMYYGHPNPYRNECVFKD
                     GSEQGVAPLPNYVPPIHTIGHNTSSNGTIEYLGDAFCGDLQGDLLIANYSVGDDLVRY
                     ELSADGGSIVGVTRLATGFTDPLPVTQSPDGTIYVGELGAGQVTALVPQDTGCWSDRA
                     PLPAQLLDVGGAALGGKFYVVAGKTSTGPKTSMYVYDPATDAWTPGPNLPGPGVENPA
                     VEALNGKLYVFGGSTDPFSGAVANASVFDPATNAWTTLAPMPTARGGANAKAIDGLIY
                     VVGGMDANGASVATVEVYNPSTNGWSAGPPMGTRRDNPGAALLDGKLYVFGGRTRESN
                     GTVLNGTLASVEMYDPGTGTWVSKAPMPTGRRTMVVGTLNGRA"
     gene            complement(6811..8646)
                     /locus_tag="VF058_00965"
     CDS             complement(6811..8646)
                     /locus_tag="VF058_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6206907.1"
                     /translation="MTDTLRKLIHLLRPEGLRRWVAVVGLAVVASGLEAGAAILIFLL
                     IGLITAPGGSIELPVIGDVTSRFDHIPRETLMFWVAGAVIVFFVLRGAFTLLQLYVQS
                     RISQNVGARLAGRLFTGYLRMPYPFHLERSSSELVRNTEETVRAITLEVLGPIVRLLS
                     SAILALAMLGVLIITAPLTTALAVAVLAPVVLLLLKVVQPRLQRWGARRQQLAKANLS
                     VVQQGLHGIRDVKLLDAVPFFSDVYLKQRRAMARVTYLGRVGMEFPSILIETVLVVFI
                     AAFFIVFLALDGSLGELLAVLGMFAYVALRLQPALQKITQSVTSLRFASRAVEHAYED
                     IVLTEAQSAEDDGDEELAPVMLRRELSLVDVSLWFPGRPEAALDDVNLTIAAGEAVGI
                     VGPTGGGKSTLLDVIAGLLRPVRGEVLVDGVPLEGRERAWQRQLGVVSQAVFMIDGSI
                     RQNIALGVADSEIDPSRLEAAVEVAQLSEFVRSLPDGLDTLVGERGVRVSGGQRQRVA
                     IARALYRDAPVLILDEGTSALDNRTEAAFMKALHRSRAGRTIIMVAHRLSTVRGCDRI
                     VLIGDGRVVDVGTFEQVAARNDGLVRVGPDPAHHGPLDGGSTIPR"
     gene            complement(8643..>9850)
                     /locus_tag="VF058_00970"
     CDS             complement(8643..>9850)
                     /locus_tag="VF058_00970"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX6206908.1"
                     /translation="EVSSRRSTRAVADWRPVEPFLRELASFGVPGGPLDPLTVPDGGW
                     PRALSGLVRERLTGIAAAALREGALKLSDQEEAELLDRQEEAMCHALALERELLRLER
                     AFADAGVELIVLKGPAVAHTIYPDPSWRPFGDLDVLVRTADWRRACSLLPELGYRRRF
                     PEPRPGFVERFGHTAAHQGPAGLEVDLHRTLVGGPFGLWMDAEELFDYTETFELGGRE
                     LRRLDDTALLMNAFVHAALGHRPPLLLPLRDVAQAASDRIDRARLADWGRRWRLAVVY
                     RYASQVLSESVGVRLPEGLVPHMEERPPRRERRALEAYTTARRERGGKLLGSLRAIRG
                     LRAKAAYLIALLFPSREFLRSRSSGDGFISYRRRWATPLRWMSQGRSGGHRSDERDVT
                     APQEMVRNP"
BASE COUNT         1471 a         3411 c         3418 g         1550 t
ORIGIN      
        1 ggcgcggccg ttgagggtcc caaccaccat cgtccgccgg ccggtcggca tcggagcttt
       61 cgacacccac gtcccggtgc ccgggtcgta catctcgacc gacgccagcg tgccgttcag
      121 cactgtgccg ttggactccc gggtccgtcc tccgaacacg tagagcttcc cgtcgagcag
      181 cgccgcaccg gggttgtcgc ggcgggtgcc catcggggga cccgccgacc agccgttggt
      241 cgaggggttg tagacctcga ccgtggccac ggacgctccg ttcgcatcca tgccgcccac
      301 gacgtagatc agcccatcga tggccttggc gttcgcgccg cccctcgcgg tcggcatggg
      361 ggcaagcgtc gtccaagcgt tcgtcgcggg atcgaacacc gacgcgttgg cgacggcccc
      421 ggagaaggga tccgtcgagc ccccgaacac gtagagcttc ccgttcagcg cctcgaccgc
      481 cgggttctcg acacccgggc ccggcaggtt gggaccgggg gtccaggcgt ccgtcgccgg
      541 atcgtacacg tacatcgagg tcttcgggcc cgtgctcgtc ttgccggcaa cgacgtagaa
      601 cttgccgccc agcgcggcgc cgcccacatc gagcagttgg gccggcagcg gcgcgcggtc
      661 gctccagcag cccgtgtcct ggggcacgag cgccgtgact tgcccggcgc cgagctcacc
      721 gacgtagatc gtgccgtccg gcgactgcgt caccgggagc gggtccgtga agccggtggc
      781 gagcctggtc actccgacga tggacccgcc gtccgccgag agctcgtacc ggaccaagtc
      841 gtcacccacc gagtagttcg cgatcaggag gtcgccctgg aggtcgccac agaacgcgtc
      901 gcccagatac tcgatcgtcc cgttcgacga cgtgttgtgg ccgatcgtgt ggatcggtgg
      961 gacgtagttc ggaagcgggg cgacgccctg ctcgcttccg tccttgaata cgcactcgtt
     1021 ccggtacggg ttcggatggc cgtagtacat cccctgctcc agccggttga tgatgtccgg
     1081 ctgctcgccg gggttgtgct ggctggcgtc ggccaatccc gtgcagtctg gcgtcggctt
     1141 cggcgggtac gtccccgtca cgccgagccc gttgtccggc gcgtagatgc tgccgttgga
     1201 gtggtgcacg aagtcgtacg tgttccggag cccgctcgcg tagacgctca catcgcaggt
     1261 cgccgggacc ccgaactcgc ccagcggcgt ggcgcacgac ccctcgaacc ccgtcgcctt
     1321 cacgtcggcc acgagcagcg cagcggagag cggctgctcg ggccggtcgc cgaactcggt
     1381 gttggcgagg ttgggcgccc ccgctcccgt gttgccgccg tgtgccatgt acaggcgccc
     1441 gtccgggccg aagtggatcg agttgatggc gtgattcgcc ttcgccctcg gcagccccgt
     1501 gatcacgtcc tctctcgctg taaagccgga ccctgagagg cgtgtgaccg tgctcgagtt
     1561 gacctccccg ttattgaccg atgggctcga gtgggacacc cagagcacga cgttgctcgc
     1621 cgtcgagtcc gggtccaccg tgatcccgag cgtcagtctg ctccccagcg tcgtgatgac
     1681 ctcgtcgctc acgacctgct gattggcgtc gagggtgacg gcgtggatct tcccgaagag
     1741 ctcggtcaca tacagacgac cgtccggtcc ccacaccatc gaggtggggt tgggcacagg
     1801 gaagctgctc ttgtcgaacg agaatccctc gggcgtcgtc gggttggcca ccgacgtgtc
     1861 ctccaccacc gagaagttgt cgaacgtgta catgagcggc gaccctcccc ggtgggtggc
     1921 gaagaccccg ccgaagctgc gcgtcccgat ccgggggtcg atgcccgccg cgtcgaagct
     1981 gaagaactcg ggcggggccg tgaagctggc caccaccttc tcctccccac cgtcgacgcg
     2041 gtaggagccc tcgatcttcc gggtgaaggg gttggcccgg aggatcagcg tggcggtcga
     2101 cgagctcaga tcgaggacgc cggtccgctt cgggctcacc ggccgagcgc cgtccacctc
     2161 gatcaggtac tcgatcacgg tcccgttcgt cgtggacagg gtgatgatct tcacgtagtt
     2221 gtcctggtcg ttcccgaacc agagaccggc ctgctcgaac ttgccggtgc ccgccggcgg
     2281 gttgacgacg tccgtctcga tgatgaacgc ctggttcggc gccgcgatcc ccactcccag
     2341 ggcgttgtcc tggccgttct cggtcccggc cgcccccccg gaggtcgtcg cgatctggaa
     2401 gacaccctgc gccgtgtcga cgctgagctt ctccggcagg tagccgttcc cgcccggatc
     2461 cacgtaggtg aacccggtcc ccacaccgtt cccggcgagg atctttccgt ggtcgacgcc
     2521 ccagtccagc cggtacggca gcgagacctt gatctcctcg cacggcaggg gcgtgcagcc
     2581 gccggtgccg atcgccagcg tcacgagcag ggtcgccgga tcgtatccgg gggccgtagc
     2641 cgtcgcggcg gcctgatagt tcccctcggg gagtcccgtg gtgtcaacct cgaaggtgag
     2701 ggtggccggg gtcgtccccg acaccggggt gaccgtgagc cagtcggcgt catcgtccac
     2761 gatgaagtcg gccgctgtgc cgtcggtcgt gatgagcccc gacgtctgtg gatcggcgct
     2821 ccctccctcc tccagcacgt cgaagagcag cgggtccgtc gtgaacgcga gggccggcgc
     2881 gttgttggcg atcgtgaagg tcgacgtgat cacctggctg ccgcctgcag agaggtccaa
     2941 ccgtgccgtg atcgtgtgct ggccgtcggg gaccgtcgtc gtgtcccagg ggaaggcggt
     3001 cccgtcttgg gctgtgcccg cgaaatcgta tggtgcgccg ttctcgacct tcgtcggtgt
     3061 gcccgtcatc tgaggatcgt cgagccagaa cgacaccttc gagacgccgt cgtcggggcc
     3121 ggtgaacacg taggcgttcc cctggagcgt ctggccctcg aggtcggcga aggtgttgcg
     3181 gtcggccgtc gtgctggaga gcagccggta gccggtccca ggtgcgagca cggtgagcgt
     3241 cacgggaagc accacgctgc cgaaccccgc cgtcgacgcg gtgaccgtcc cgttgtacgt
     3301 ccccgccgcg aggcccgtcg tgtcggcggt cacggtgatc gccgacggcg tcgtgcccga
     3361 ggccggcgcc gcggtcagcc acggtcggtc actcgtgacg tcgtacgccg tctgcgtgcc
     3421 gtcagtgacc tcgagctgca ccgtcgcgtt cggagcgtcc ccctgctctc cggagaggct
     3481 gaggctggtc ggctcgaacc gaagctgcgg cgcatcgttc gccaccgcga agctcgcgtg
     3541 gacggtgtcg ctccctcccg cggagagctc gatcagcgcg ctgatggtgt gggacccatc
     3601 gttgaccgca gtggtgtccc agggaagggc cgtgccgttc ttcgccgtcc ccgcgaagtc
     3661 gtacggcgcc ccattctcga ccttggtcgg ggttccggtc atggccgtgt tgtccagcca
     3721 gaaggacacc ttcttgaccc cgtcgtcggg gcccgtgaac acgtaggcgt tcccctggag
     3781 cgtctggcct tccagatcgg cgaagccgga ccggtcgggc gacgggctga cgagcaaccg
     3841 atatcccgtg tctgccagcc cgaccgtcag ggctacgggg aggctcaccg ggtcgaggcc
     3901 ggaggcggtg gccgtcaccg ttccgttgta cgtaccgagc gcgagccccg tcgtgtcggc
     3961 ggtgaccgtg atcgcctgag gcgtggtgcc ggaggccggc tcggcggtca gccacggctt
     4021 gtcggtgccg agcgagtagc tcgcggccga gccgtcggtg acgctcaggt cgaccgtcgc
     4081 gctgggggcg tcgccctcgc tgcctgagag gctcaaggtc cccggctgga accggagctg
     4141 cgggccgtga ttcgagacgg tgaacgtcgc gtgcaccacc tcactcgttc cgctggagag
     4201 atcgagccgt gccgtaatcg tgtgtgagcc gtccgccacg gtggtcgtgt cccaggggaa
     4261 ggcggtgtcg tccgcggccg tgccgttgaa gtcgaagggg gcccggctct cgctcttgaa
     4321 gggcgtggtc gtcatgctcg tgtcgtcgag ccagaacacg accctggaga ccccatcgtc
     4381 ggggccggtg aacacgtagg cgtccccctg gagcgtcgtc ccctcgagca ccgtcgggtt
     4441 gctgcgagtc ggcgaaccgc tgcggaagag ctgatagccg gtgcccgccg atgacacgga
     4501 gagcgagacg gggacgctca ccgggtcgta cccggaggcc gtcgcggtga gggttgcctg
     4561 gtgcgtgccg ggagcaaggc tcgaggcgtc cgcctcgacc gacagggtgg ccggcgtgga
     4621 tccggacagc gggctgaccg tcagccaggg ggcgtccgtc gcgacgtcga aggacgccgt
     4681 cgaaccgtcg ctgacctcga ggtcgacggc ggcggtgact gtctcaccct cccccgcgct
     4741 caggttgagc gccccggggc tcgtcttcag ctcaccgggg cacgcgccgt cccggtactc
     4801 gaaggcatcg atcatcgtcg gaggggtccc gtcgcccgtg aagccgtacg tctcgacccg
     4861 gaggaccccc tgcgaggaca cctccagccg ggagaagtgg tgcagcgtgt tgtcgcggaa
     4921 cgccgtccag tgcggcggcg ggttcgtcgc gaactgggag aaatcgccgt tcttgttgct
     4981 cagcttgccc gccgggctga ccttcacgta ggtcacgccg tcggccatcg tgtagcagga
     5041 caaggaggac gaggtcgggg tgttcgtggc gggtacgtcg gtcaggggat acgtccgctc
     5101 gtaggactga tcgtgggagg tcaacacgac ctgcaccccg agctgctcga agagcgggcc
     5161 gagctgacct cgtaggttca ggttcgacga gtggttcttc ccgtccgaga acggaggcac
     5221 gtgcatgtag ggaacgatcc agcgctgccc cgccgccttc gccgcctcga tatcctgccg
     5281 gatccagtcg agcgcggcgg aggacaggcc ctgcgtctcg gccacggcca ggatcgacac
     5341 gaagtggacg tccccgacgt cgaacgagta gaagcgacgg ccgtcgaacc cctggggcgt
     5401 cgggaatcgg gacgcccaag ccgagtagct ctccccgagg agcacctcgt ggttcccgta
     5461 ggtcggcatc atcggagcgc gggtggcgaa gggctcgttc tgcgcgaacc aggcgtcgat
     5521 cgagtcgtcg agggacccgt acctcgtgtc ggtgttgtag tagatgtagt cgcccccggg
     5581 gaggaacagg gtcgggtcga gcgcggtcat ctcctggatg gcctgctcgg tgcccgtagc
     5641 cagaccgtcc tcgcgaccaa tcagcccggt gtcggcgaag tagatggccg agaagtcggc
     5701 cggacccggc gcgggggcgg tccgagtcgt gtgcacggtg ctccagccct cgctcccgcc
     5761 gtccacccga tactcgtagg tggtggcggg gacgagaccg gtgagcgtcg cctcgcggag
     5821 ggtccccgtc gtccccgacg tccgggtgcc gccggtcgct tggagccagg tggaggaacc
     5881 ctgctcccgg tactcgacca ccgaggaggc gtcgggcgcc agcgtccgcc acaccaccgt
     5941 catggtcgtc gaggggtcgt cgacccaggc gagatggatc tgatccgcgg ggcccagatc
     6001 acccacggtg agcgtcacgg gcaggaccgc tggggtgtgg ccgtccgcgg tcgcggtgac
     6061 cctgccgctg tatgccccgt cggcgagccc gctcgcgtcc accgacacgg tgacggtcgc
     6121 ggggtaggtg ccactcgagg gggaggccga cagccacggg gcgtcggcgg acacctggaa
     6181 cgatcccggg tccggccccg aggaggcgag ctccacgctc gtcgaatcgg ttccaccgct
     6241 ctgcacggcg aggtccaccg acgcgggcgt gaacgagagc tgagcgtccg cgttctggac
     6301 ggtgaaggac gcgtggacca ccgtcgtccc gccggccgag aggtcgatcg ccgcgctgat
     6361 ggtgtgcggc ccgtcggcga ccgcggtcga gtcgaacggc ttcgcggtcc catcgctcgt
     6421 gaccgtcccc tcgaagtccc acggcgcgcg cttctcggtc gtcctcggct tcccgctcat
     6481 cgcggtgttg tccagccaga accgcacctg cttgatcccg gactcgggat ccacgaagac
     6541 atagatcgtg ccgccgacca ccgccccgtc gagggcgccc ggtgccgacc gatcgggcga
     6601 gctgctgacg agcaacccgt gcgaagcctg cgcctgggac gcaggcgcga gcgacgttat
     6661 cagcagggcg agcccgagga ggagcaccgt cgcgctcatc aaccttcgtc gccgttggta
     6721 cctacctgcg cctcgcttca tggtgatctc catcgggccc cctgggcggt tggttccggt
     6781 cacgtcgttg tgcaggaaag tgcgggcggt tcagcgcggg atcgtcgacc cgccgtcgag
     6841 cgggccgtgg tgggcggggt ccgggccgac acgcaccaga ccgtcgttcc gagccgccac
     6901 ctgttcgaag gtgccgacgt cgacgacgcg gccgtccccg atcaggacga tccgatcgca
     6961 gccccgcacg gtcgagagcc ggtgggcgac catgatgatc gtccgacccg ccctcgaccg
     7021 gtgcagcgcc ttcatgaacg cggcctccgt gcggttgtcg agcgcggagg tcccctcgtc
     7081 gaggatcagc accggcgcgt cccggtaaag ggctcgggcg atcgccacgc gctgccgctg
     7141 gccgcccgag acccgaaccc ctcgctcccc aaccagggtg tctaggccat ccggcagact
     7201 gcggacgaac tccgagagct gagccacctc caccgccgcc tccagcctcg atgggtcgat
     7261 ctcggaatcg gccacaccga gggcgatgtt ctgtcggatg ctgccgtcga tcatgaacac
     7321 cgcctgagag acgacgccga gctgccgctg ccaggcgcgc tcccggccct ccagcggcac
     7381 gccgtcgacc agaacctccc ccctgacggg cctgaggagg cccgcgatga cgtccaggag
     7441 tgtgctcttc cctccgcccg tgggcccgac gatcccgacg gcctccccgg ccgcgatcgt
     7501 gagattcacg tcgtcgagtg cggcctctgg acgaccgggg aaccagaggc tgacgtccac
     7561 gagcgacagc tcccgccgaa gcatgaccgg ggccagctcc tcgtccccgt cgtcctcggc
     7621 cgactgcgcc tcggtgagga cgatgtcctc gtaggcatgc tcgacggcac ggctggcgaa
     7681 ccgtagcgag gtcacgctct gggtgatctt ctggagcgcc ggctgcagcc gtagcgccac
     7741 gtacgcgaac atccccagga cggcgagaag ttcccccagg ctcccgtcca gcgccaggaa
     7801 cacgatgaag aaggccgcga tgaagacgac gagcacggtc tcgatgagga tcgaggggaa
     7861 ctccatccct acccgtccca ggtacgtcac gcgcgccatg gcccgtcgct gtttgaggta
     7921 gacgtcggag aagaacggca cggcgtccag cagcttcaca tcgcggatcc cgtgcaagcc
     7981 ctgctggacg actgagaggt tggccttcgc gagctgctgg cgccgcgcgc cccaccgctg
     8041 gaggcggggc tggaccacct tcagcagcag cagcaccacc ggcgccagca ccgccaccgc
     8101 caaggcggtc gtcagcggcg cagtgatgat cagcaccccc agcatggcca gcgcgaggat
     8161 ggcgctggag agcagccgaa cgatggggcc caggacctcc agcgtgatgg ctctgacggt
     8221 ttcctcggtg ttccgcacca gctccgagga gctccgctcg agatggaacg gatacggcat
     8281 ccgaagatag ccggtgaaca gccgccccgc gaggcgagcc ccgacgttct gggagatcct
     8341 gctctggacg tagagctgga ggagcgtgaa cgcgccccgc aggacgaaga acacgatgac
     8401 cgctccggca acccagaaca tcagcgtctc acgcgggatg tggtcgaagc gcgaggtgac
     8461 gtcgccgatc accgggagct ctatcgagcc acccggtgcc gtgatgaggc cgatcagcag
     8521 gaagatgagg atcgcggccc ccgcctcgag gccactcgcc accacggcga ggccgaccac
     8581 cgcgacccat cggcgcaggc cctccggccg aaggaggtgg atcagcttcc gaagggtgtc
     8641 cgtcatgggt tcctcaccat ctcctgcggt gcggtcacat cccgctcgtc ggagcgatgc
     8701 cccccggagc ggccctgcga catccagcgg agcggcgtcg cccaccgcct gcggtacgag
     8761 atgaagccgt ctccgctcga tcggctccgg aggaactctc ggctggggaa gagcagggcg
     8821 atcaggtacg ccgccttggc ccgaaggccg cggatcgctc ggagcgaccc cagcaacttc
     8881 cctcctcgct cgcgtcgggc cgtcgtgtac gcctccagcg cccggcgctc gcggcgcggc
     8941 ggccgctcct ccatatgggg aaccagtccc tcgggcagtc gcacacccac cgactcagac
     9001 agcacctgcg aggcgtaccg atagacgacc gccagccgcc agcgccggcc ccagtcggcg
     9061 agccgcgccc gatcgatccg atccgacgcc gcctgggcca cgtcccgaag cggaaggagc
     9121 agggggggac ggtgcccgag ggccgcgtgg acgaaggcgt tcatcagcaa cgcggtgtcg
     9181 tcgagccgcc ggagctcgcg gccccccagc tcgaacgtct ccgtgtagtc gaacagctcc
     9241 tcggcgtcca tccagaggcc gaacggcccc cccaccagcg tccggtgaag gtcgacctcg
     9301 aggcccgccg gcccttggtg tgcggcggtg tgcccgaacc gctccacgaa tccgggacgc
     9361 ggctcgggga accgtcgtcg gtagccgagc tcgggcagga gcgagcaggc ccgtcgccaa
     9421 tcagccgtcc gcaccagaac gtccaggtcg ccgaaggggc gccacgaggg atcaggatag
     9481 atggtgtggg cgacggccgg tcccttgaga acgatgagct cgacgccggc gtccgcgaag
     9541 gcgcgctcca gccgcagcag ctcccgctcc agggccagcg cgtgacacat cgcctcctcc
     9601 tgccgatcga ggagttccgc ctcctcctga tcggagagct tcagcgctcc ctccctcaag
     9661 gcggccgccg cgatgccggt gaggcgttcc ctgacgagcc cgctgagtgc cctgggccac
     9721 ccgccgtccg gaacggtcaa ggggtcgagg ggaccgcccg ggacaccgaa cgacgccagc
     9781 tcacggagga agggctctac cggccgccag tcagccacgg ctcgcgtcga cctcctcgat
     9841 gagacctcgc
//
LOCUS       DASUTW010000026        12043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_526652, whole genome shotgun sequence.
ACCESSION   DASUTW010000026 DASUTW010000000
VERSION     DASUTW010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12043
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526652"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VF058_00975"
     CDS             complement(<1..741)
                     /locus_tag="VF058_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206909.1"
                     /translation="MRRVAMWGLAGIGAVCLVVTVVVGLSLDLPPGVAAWIFVGPLPI
                     LAMGAFLYWRRPDHRTSQQFLVWAVVTFCLAMPIEAITVAVAEDGSPPSWTWLAVLVF
                     MTASVAGAVLGATLIGLLPDGRFRHPWERPILLPMWGVLVVPMLAFLTNERVPLPGYV
                     FPGIEEASSPLFVPSLAALGPLAITLNALYSAVFVGGMGLLVVRYRKGSARERRQLRW
                     VLLAAAVTVGAAIWPFLLGELGVLPAVTH"
     gene            928..1371
                     /locus_tag="VF058_00980"
     CDS             928..1371
                     /locus_tag="VF058_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206910.1"
                     /translation="MRRRAGDGITRERVGRALFGAVSAALLLSGAYLIVNTVPDEGDL
                     RRSGISWEELLANRPAVAAYLERLLRLAGVAAAGLGTMALAGVLSMRSGGRPVWRMLW
                     TLPAITAAITSVLVAEGTPIAWYYGGLTLLATAGLVLARPGKEEG"
     gene            1368..2015
                     /locus_tag="VF058_00985"
     CDS             1368..2015
                     /locus_tag="VF058_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206911.1"
                     /translation="MTDLDRSSTMTVTRIAPATGLAFVALALAAALVIGQYSYLPSGD
                     EIAAFFQEHARRIQIGAYLGALSSVFLIWFSGSVRSHLRVAEGGTGRLSAIAFGGGVA
                     AAVILGIAFSILAVAGARGGEGEVTPATAMVLYDVYGSLAGVAAPVAMGALVGAAGLL
                     GIRTGALAGWLSWSGAVLAFGLISPFSYIFIAIAFLWIAALSILLLGSEGATTSR"
     gene            2012..3205
                     /locus_tag="VF058_00990"
     CDS             2012..3205
                     /locus_tag="VF058_00990"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6206912.1"
                     /translation="MTLSAEELAARAGTTAEQISRHADLRILPSEPPFEETLVPRARL
                     ALELEASGIPLEQIGRRSGRDGFSLSFADRVFGRPVAMLPTTYAELLDELRMSERLFD
                     EVLISLGMAADEPVREDDAELLRLLDRMIKLGLDEPSLTRFLHIVVDSTRRMADAGRE
                     MWRTGVEAPLLASGLSHHELLHASSDPAPESQVIGDRLIGILWNRFIEQVVVQASIEH
                     LELALEEAGVAFRRNPRPPAIAFVDLSGYTQMTDRVGDDAAAEHARSMLDVVRRTIGG
                     GQGRLVKMLGDGAMLHFSEAGAAVTCGLRLIDRIPRSGLPPARLGIAAGPLIVRDADY
                     FGRTVNVAARLADYARPSEVLVTSSVVDAAGNAAASFREIGPVALKGVSEPVTAFSAQ
                     PTEGS"
     gene            3211..4449
                     /locus_tag="VF058_00995"
     CDS             3211..4449
                     /locus_tag="VF058_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206913.1"
                     /translation="MGITRVRSEAVWGHLRVLFTGAALLFLINIWFGFDNALTSGGIP
                     RWQALIHLHAGSIGWITLSAIGIGIWVFTGSRVVSREYERRVGLLVWTAVLVFAGYIP
                     NFWLGFSRGDGPLVALLPVFGSLAVIVLWVSAIFVLRQLRHQEVVTTVHILASAALLV
                     AAIGATMGALLGLERVIGEFLPIADPDRVGVHAAMMDTYLFLVAAAIIEWAITLEARR
                     WTRPGLAQGLAWGVAATLVPLGFLLDLLDQLLPVFGLLLLLGLVLFLGRVAWRALAIG
                     PGAEDPTARWAFFGTAWLLVFLGLFLYAVSTGGEFDSLPSWFGAAFVHAGFVGMMTNL
                     ILGVLSARTRERRHVVAWGEPAALWTINLGLVLFLTLEIAADVRIGALVMGVGVLLGV
                     GTMLLRLGASQRTSQWERVA"
     gene            complement(4451..5683)
                     /locus_tag="VF058_01000"
     CDS             complement(4451..5683)
                     /locus_tag="VF058_01000"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX6206914.1"
                     /translation="MGERVDVVVVGAGHAGLSISNELSRSGVEHLVLERARIGESWRG
                     RWDSFCLVTPNWSVQLPEHPYDGDDPDGFMLRDEIVAYLERYAAAVGAPIRDQTEVEN
                     LDPATDGGFALRTSGGEIRARSVVIASGAFQRPRRPRGADDLPAEIPQLDVDGYRNPG
                     ELPSGGVLIVGSGQTGCQIAEELLGAGREVFLACGRAPWAPRRLGDHDLVWWLAETGF
                     LDQTPEDVAWPAARRAANVIGTGAGGGHDLNLRRLRAAGATLLGHFLGAEGRCARFAD
                     DLEESVAWGDDRYRDLMGLFSKLVAERGLPDPGFADPPPFEGSAPTDLDLGRIGSVIF
                     TGGFRPDYRRWVHVPDAFDEAGFPLHVDGASSAVEGLYFVGVHFLRRRKSSLLIGAGE
                     DAAVVGRRIAAVLGASAD"
     gene            complement(5870..6640)
                     /locus_tag="VF058_01005"
     CDS             complement(5870..6640)
                     /locus_tag="VF058_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX6206915.1"
                     /translation="MVRRAMVLAYGAIAYVAFLGVFISTIGFLANVSPKGIDEGASGP
                     AGGAVLVNVGLLALFAVQHSVMARSWFKRWWTRFVPASIERSTFVLSASLVLALLLWQ
                     WRPLPASVWSVGEPWARTVLWGLYGLGWLIVVGSTFLIDHFDLFGLRQVVARARERPL
                     EPVGFRQPLLYGLVRHPIMVGFLIAFWSGPDMSVGRMLFALLGTGYILIGVRFEERDL
                     VASHGAAYERYAEHVPRFVPRLGPGSPAGRRAVGELRR"
     gene            complement(6693..8354)
                     /locus_tag="VF058_01010"
     CDS             complement(6693..8354)
                     /locus_tag="VF058_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6206916.1"
                     /translation="MANQVDDLFAAGNEALASGDWASARDSFRSALEHGETAEGLVGL
                     ADALWWLGEIQDAVSFRERAYAAFRRRPDPVSAALVAVRLCVDHRANFGNPTASGGWR
                     SRAARLVEEFELEPLRGWILLLEAYDGDDPRLGEAKAREARELAAASGDLDLELCALS
                     QVGASLIDQGKVEEGVVLLDEAMAGSLGGEGGSRDTVVFTSCHMIGSCTRCAEFERAV
                     HWVRAADRFTERFGCPFLFAFCRTLYGSVLFATGDWEGAERELHTAIEMSRGSLSALH
                     GGALASLAELRLAQGRVEEAERLVADFQGHAVAAPIVAAIHMARGEFAAAAATIERRI
                     DDVGEDRLESSLLVELLGESAIARGETAEAERRARALAERGVSLGCEVMVARAERLLG
                     HALAAGGDEPAGARHLTTALAAFVALEMPFETARTKRLLAETIRDSDPEVAVAEARAA
                     LAAFEDLGAGTDADATAALLRDLGVKAARAGPKGLGALTKREQEVLGLLAEGLSNPEI
                     AERLYLSRKTVEHHVARILSKLGVRGRAEAAVEAVRRLGAGSAEN"
     gene            complement(8648..9265)
                     /locus_tag="VF058_01015"
     CDS             complement(8648..9265)
                     /locus_tag="VF058_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206917.1"
                     /translation="MTGHENEVEASLRELLREKAGSLPPPSGLSPPALRRTRLRIART
                     VTAAALVLGLSGLGAAAVLERVTGTGEVRPGGTSFVQGATLRVRFDGSLGDLGCSHDG
                     PARVSARDEVTFVLTNDGRVDVVLDVLRFAEGRDFDDLAAYVEDAQEDPRVAPEWAAS
                     QEAFRSSPGQRESWTRALSPGDYGVVCYTADPPRLWLLSPLTVAD"
     gene            complement(9267..9803)
                     /locus_tag="VF058_01020"
     CDS             complement(9267..9803)
                     /locus_tag="VF058_01020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEX6206918.1"
                     /translation="MGEGTGAEWSPELRRWVATEWGRLGELYARHSSDAYRLAYLVTG
                     DQPLAEDLVQEAFINVAGRLVHLRHPDAFHAYLRRTVLNLCKMHFRRRQVERAYLQRE
                     GHRIEEGDPTLASDLREDLWSELQRLPHRQRVAVVLRYYEGLTDHEAAQILECRPGTV
                     RSLLSRAMSVLRTRVRTG"
     gene            10127..10447
                     /locus_tag="VF058_01025"
     CDS             10127..10447
                     /locus_tag="VF058_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206919.1"
                     /translation="METRPRRTHRRRGLGATLVLVALALLLSSCAAGPNPATGTGEDP
                     AGFLLGLWHGIILPVTFIISLFTDEVSVYEVVNSGNWYDFGFILGVLISLGGGGAGAG
                     RRRG"
     gene            10419..10895
                     /locus_tag="VF058_01030"
     CDS             10419..10895
                     /locus_tag="VF058_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007462855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX6206920.1"
                     /translation="MRAPAGGEADGPEDEEVFDSPSGWVRKHVREYVETDGRKGHRWS
                     GVHTLLLTTRGRKSGKLRRTALIYGRDGDRYLVVASKGGAESHPSWYLNLSEHPEVRV
                     QVGSDRFVARARTADTEEKLRLWRIMTEIWPEYDRYQERTAREIPVVVLEPVAERA"
     gene            11120..11307
                     /locus_tag="VF058_01035"
                     /pseudo
     CDS             11120..11307
                     /locus_tag="VF058_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882992.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            11416..11868
                     /locus_tag="VF058_01040"
     CDS             11416..11868
                     /locus_tag="VF058_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206921.1"
                     /translation="MKERFTEEEWRGIMALPFAIFGVVAGADGNIDEQEIGEFTKRML
                     NPTWTYKDAMHQEVAVAVAGDDVSALIKEGMALDPEPVKTALKEKLTADEYQSFLGSV
                     FIDELAVARASGGGIFHKEKIADKETEKLVGFAAYFGIDLPSVQRIFS"
BASE COUNT         1784 a         4381 c         4122 g         1756 t
ORIGIN      
        1 gtgggtcacc gcgggcagga cgccgagctc ccccaggagg aacggccaga tcgccgcgcc
       61 gacggtcacc gccgcggcca gcagcaccca cctgagctgc ctccgctctc gcgcggagcc
      121 cttccgatag cggacgacga gaagccccat gccccccacg aacaccgcgg agtagagggc
      181 gttgagcgtg atcgcgagcg gaccgagcgc cgcgagcgag ggcacgaaga gggggctgct
      241 cgcctcctcg atccccggga acacgtagcc ggggagcggc acgcgctcgt tggtgaggaa
      301 ggcgagcatc ggcacgacga gcacgcccca catcgggagg aggatggggc gctcccaggg
      361 atgccggaac ctcccgtccg gcagcagccc gatcagcgtc gcccccaaca cggcccccgc
      421 gaccgaggcc gtcatgaaga cgagcaccgc gagccaggtc cacgagggcg gggagccgtc
      481 ctccgcgacg gccaccgtga tggcttcgat cggcatggcc aggcagaagg tcacgacggc
      541 ccacacgagg aactgctggg aggttcggtg gtccgggcgg cgccagtaga ggaacgcgcc
      601 catcgcgagg atcggtagcg ggccgacgaa gatccacgcg gcgacgcccg gggggagatc
      661 cagcgagagc cctacgacca cagtgacgac caggcagacg gcgccgatgc cggcgagccc
      721 ccacatcgcc acccttctca tggcccccaa gcctcctcgt cgaggggtga ggccaccagc
      781 ctaatccctg ggtcacggcg tcgaacatcc cgtgggccac cgttccgagg tagggacaac
      841 ctcacccgag aactgaagca ggcaggcttc cgaggacagg cctcgcgggc ctagcctgac
      901 cccgccatgg tcacgatgcg acggagcgtg aggcggcgcg ccggagatgg gatcacgcgc
      961 gaacgagtcg gtcgggcgct cttcggggcg gtctccgccg ccctcctgct ctcgggcgcc
     1021 tacctgatcg tgaacaccgt gcccgatgag ggcgacctcc ggcgcagtgg gatctcctgg
     1081 gaggagctcc tcgcgaaccg gcccgccgtg gccgcctacc tcgaacggct cctccgcctg
     1141 gcgggggtgg ccgccgcagg cctggggacg atggccttgg cgggcgtcct gtcgatgcgt
     1201 tcggggggcc ggcccgtgtg gcggatgctg tggacgctcc cggccatcac cgctgcgatc
     1261 acctcggtgc tcgtggcgga ggggaccccg atcgcttggt actacggagg cctgacgctg
     1321 ctggccacgg ccggcctcgt cctggctcgc cccgggaagg aggagggatg acggatctcg
     1381 accgaagcag cacgatgacc gtgacccgga tcgcaccggc gacgggactg gctttcgtgg
     1441 cgctggccct ggcggcggcc ctcgtgatcg gccagtacag ctatctcccc tccggcgacg
     1501 agatcgccgc gttcttccag gagcacgcgc ggcgcataca gatcggcgcc tatctaggag
     1561 cgctctcctc cgtgttcctg atctggttct cggggagcgt ccggagccat ctcagggtcg
     1621 ccgagggcgg caccggccgc ctgtccgcga tcgcgttcgg cggcggcgtg gctgcggccg
     1681 tcatcttggg gatcgccttc tcgatcctcg cggtggcggg agcgcggggc ggcgagggcg
     1741 aggtcacgcc cgcgacggcc atggtcctct acgacgtcta cggatcgctt gcgggagtgg
     1801 ccgctcctgt ggcgatgggc gcccttgtcg gggcggccgg ccttctcggc atccgcacgg
     1861 gggcgctcgc cggctggctc tcgtggtcgg gggcggtgct ggccttcggg ctgatctctc
     1921 ctttctccta catcttcatc gccatcgcgt tcctgtggat cgccgccctt tcgatcctcc
     1981 tcctgggcag cgagggggcg accacctctc gatgacgctc tcggcggagg agctggccgc
     2041 gcgggcgggg acgacggccg agcagatcag ccggcacgcc gacctccgga tcctcccctc
     2101 cgagcccccc ttcgaggaga ccctggtccc gcgggcccga ctggccttgg agctcgaggc
     2161 ctccggcatc ccgctggagc agatcgggcg ccggagcggc cgggacgggt tctcgctctc
     2221 gttcgcggac cgcgtgttcg ggcggcccgt cgcgatgctg cccaccacgt acgcggagct
     2281 cctcgatgag ctccggatgt ccgagcgcct cttcgacgag gtgctcatct ccctcggtat
     2341 ggcggccgac gagccggttc gagaggacga tgcggagctc ctccgcctcc tcgaccgcat
     2401 gatcaagctg gggctcgacg agccgtcgct cacccggttc ctacacatcg tggtggacag
     2461 caccaggcgc atggcggacg cgggacggga gatgtggagg accggggtcg aggcgccgct
     2521 cctcgcctcc gggctctccc accacgagct gctgcacgcg tcgtcggacc cggcacccga
     2581 gtcgcaggtc atcggcgacc ggctgatcgg gatcctctgg aaccggttca tcgagcaggt
     2641 cgtggtccag gcgtcgatcg agcatctcga gctcgcgctg gaggaggcgg gcgtcgcgtt
     2701 ccgccggaac cccaggcccc ccgcgatcgc gttcgtcgac ctctcgggct acacgcagat
     2761 gaccgaccgg gtcggggacg acgcggcggc cgagcacgcc cgatcgatgc tcgacgtggt
     2821 caggcgcacg atcggcggcg gccagggacg cctggtgaag atgctgggcg acggcgccat
     2881 gctccacttt tccgaagcgg gggcggccgt gacctgtggg ctccggctga tcgaccgcat
     2941 cccgcgatcg ggccttcccc cagcccgcct cgggatcgcc gccgggccgc tgatcgtccg
     3001 ggacgcggac tacttcggaa ggaccgtgaa cgtggcggcc cgcctggcgg actacgcgcg
     3061 tccgagcgag gtcctcgtga cgagctcggt ggtcgacgcc gccggcaacg ccgccgcctc
     3121 gttccgcgag atcggtcccg tggccctcaa gggcgtgtcc gagcccgtga ccgcgttctc
     3181 cgcccaaccg acggaaggga gctgagaccg atgggcatca cccgggttcg gagcgaggcc
     3241 gtgtgggggc atctgcgtgt cttgttcacg ggcgccgcgc tcctgttcct catcaacatc
     3301 tggttcgggt tcgacaacgc gctcacgtcc gggggcatcc cacggtggca ggcgctgatc
     3361 cacctgcacg cgggcagcat cggatggatc acgctctcgg cgatcgggat cggcatctgg
     3421 gtcttcacgg gcagccgggt ggtctcgcgg gagtacgagc gaagggtcgg cctcctcgtc
     3481 tggaccgcgg tgctggtctt cgctggctac atccccaact tctggctggg cttctcgcgg
     3541 ggagacggcc cgctcgtggc cctgcttccc gtcttcggaa gcctcgcggt gatcgtgctg
     3601 tgggtctccg cgatcttcgt cctgcggcag ctccgccacc aggaggtcgt cacgacggtc
     3661 cacatcttgg cctcggcggc gctgctcgtg gccgcgatcg gggcgacgat gggcgccctg
     3721 ctcggcctag aacgggtgat cggggagttc ctgccgatcg cggatcccga ccgcgtgggc
     3781 gtccacgccg cgatgatgga cacctacctg ttcctggtcg ccgccgcgat catcgaatgg
     3841 gccatcaccc tcgaggcccg gcgctggacg cgtcccggat tggcccaagg gctggcgtgg
     3901 ggcgtggccg cgacgctcgt tcccctgggg ttcctgctcg acctcctcga ccagctcctc
     3961 cccgtgttcg gcctgctgct gctcctcggt ctcgtcctct tcctcggccg ggtggcctgg
     4021 cgggcgctgg cgatcgggcc gggcgcggag gacccgaccg cccggtgggc cttcttcggc
     4081 acggcgtggc tcttggtctt cctcgggctc ttcctgtacg cggtgagcac cgggggcgaa
     4141 ttcgattcgc tgccctcgtg gttcggggcc gcattcgtgc acgccggctt cgtcggcatg
     4201 atgacgaacc tcatcctggg cgtgctgtcg gcgcgcacga gggagcgacg gcacgtcgtt
     4261 gcctggggcg agccggccgc gctgtggacg atcaacctcg gcctcgtgct gttcctgacg
     4321 ctcgagatcg cggccgacgt ccgcatcggg gctctggtca tgggcgtcgg cgtcctcctg
     4381 ggcgtgggga cgatgctgct gcgcttgggg gcgagccagc ggacgagtca gtgggagcga
     4441 gtcgcctagc ctagtccgct gaggctccga gaaccgcggc gatccgcctc ccgaccaccg
     4501 cagcgtcctc ccctgccccg atcagaagtg aggatttccg cctccgcagg aagtgcaccc
     4561 ccacgaagta cagaccctcc accgccgagc tcgccccgtc gacgtggagc gggaatccgg
     4621 cctcgtcgaa tgcgtcgggc acgtgcaccc accgccggta atcggggcgg aagccccccg
     4681 tgaagatcac cgatccgatc cgtcccagat cgaggtccgt cggcgccgac ccttcgaacg
     4741 ggggcggatc ggcgaatccc gggtccggca gtccccgctc ggccacgagt ttgctgaaga
     4801 ggcccatcag atctcgatag cggtcgtcgc cccacgccac gctctcttcg aggtcgtcgg
     4861 cgaaccgcgc acagcggccc tccgcaccca ggaagtggcc gagcagggtc gcgccggccg
     4921 cgcggagcct ccgcagattc agatcgtgcc cgcctcctgc acccgttccg atgacattgg
     4981 cggcacgtcg ggccgccggc catgcgacgt cctcgggcgt ctggtcgagg aagcccgtct
     5041 ccgccaacca ccagaccagg tcgtggtcgc cgaggcgacg gggggcccac ggcgcgcggc
     5101 cgcaggccaa gaagacctcc cggcccgccc ccagcagctc ctccgcgatc tggcagcccg
     5161 tctgcccgct gcccacgatg agcactccac ccgaggggag ctctccgggg ttcctgtagc
     5221 catccacgtc gagctggggg atctcggctg gcaggtcgtc ggcaccacgg ggccgtcgtg
     5281 gccgctggaa cgcgccggag gcgatcacca ccgatctcgc gcgaatctcc cctccggagg
     5341 tccggagagc gaacccgccg tcggtcgcag ggtcgaggtt ttcgacctcg gtctgatccc
     5401 ggatcggcgc gccgaccgcg gccgcgtatc gctcgaggta cgcgacgatc tcgtcccgga
     5461 gcatgaagcc gtcggggtcg tcaccgtcgt agggatgctc cggaagctgg accgaccagt
     5521 tgggcgtcac gaggcagaag ctgtcccacc gtccgcgcca gctctcaccg atccgagccc
     5581 gctcgaggac gagatgctcc acccccgagc gcgagagctc gttgctgatc gagaggcccg
     5641 catggccggc gccgacgacc acaacatcca cccgctctcc cattcaatcc accttccgga
     5701 gcgtcagtcg atgtacttcg ttcttcatgc tacgcgccgg cgcctccccg ggaggaacgg
     5761 ctcgagcagg ccctccccga tggcgaagat ccctcgccag gggaacagca agtcccgaat
     5821 agcgtggaca gggccccctg gagccgccgt acgatcgatc cattatcctt catcgtcgca
     5881 gctcccccac tgcgcgtcgt cccgccggcg acccggggcc cagccggggg acgaaccggg
     5941 gcacatgttc cgcgtagcgc tcgtacgccg caccgtggga ggcgaccaga tcccgctcct
     6001 cgaagcggac gccgatcagg atgtacccgg tcccgagcag cgcgaagagc atccgcccca
     6061 cgctcatgtc cggccccgac cagaacgcga tcaggaagcc gaccatgatc ggatggcgca
     6121 ccagcccata gaggaggggc tgccggaagc ccaccggctc gaggggacgc tcccgcgccc
     6181 gcgccaccac ctgccgcagc ccgaacaggt cgaagtgatc gatgaggaac gtgctcccga
     6241 cgacgatcag ccagccgagc ccgtacagac cccagagcac cgtgcgtgcc cagggctccc
     6301 cgaccgacca cacggacgcc gggagcggcc gccactgcca cagcagcagc gccagcacga
     6361 ggctcgccga caggacgaac gtgctgcgct cgatcgacgc cgggacgaac ctcgtccacc
     6421 agcgcttgaa ccacgaccga gccatgacgc tgtgctggac ggcgaaaagc gccaggagac
     6481 caacgttgac gaggaccgcc ccaccggcgg ggccggatgc cccctcgtcg atccccttgg
     6541 ggctcacgtt cgccaggaag ccgatcgttg agatgaagac gcccaggaac gcgacgtagg
     6601 cgatcgcgcc gtaggcgagg accatcgctc gacgaaccat gacgaggggc acggtcgcat
     6661 gcgggaggga cggcgagcat cggcgagctc acctagtttt cggctgatcc cgcccccagc
     6721 cgtcgcaccg cctcgacggc ggcctcggcc cggcccctca cgcccagctt ggacaagatc
     6781 cgggcgacgt gatgctccac ggtcttcctg ctgaggtaca ggcgctccgc gatctcggga
     6841 ttggacagcc cctcagcgag gagaccgagc acctcctgct cgcgcttcgt cagggccccc
     6901 agccccttcg gcccggcccg agcggccttc acgccgaggt cccggagcag cgccgccgtc
     6961 gcgtccgcgt ccgtcccggc tccgaggtcc tcgaacgcgg ccagcgccgc gcgcgcctcc
     7021 gccacggcca cctccgggtc cgagtcccga atcgtctccg ccagcaggcg cttcgtccga
     7081 gcggtctcga agggcatctc cagggccacg aacgcggcca acgccgtcgt gaggtggcgg
     7141 gcgcccgcgg gctcgtctcc tcccgcggcg agcgcgtgcc cgaggagccg ctccgccctc
     7201 gcgaccatca cctcgcagcc caggctcacg ccgcgttccg ccagcgctcg cgccctccgc
     7261 tcggcctccg cggtctctcc gcgggcgatc gcgctctccc cgagcagctc caccagcagc
     7321 gagctctcca gccgatcctc ccccacgtcg tcgatccgcc gctcgatcgt cgcggcggcc
     7381 gcagcgaact ccccccgagc catgtggatc gccgcgacga tgggggcagc caccgcgtgt
     7441 ccctggaagt cggccacgag gcgctccgcc tcttcgacac ggccctgcgc gagccgcagc
     7501 tcggccagcg aggcgagcgc cccgccgtgg agcgccgaga gcgagccccg ggacatctcg
     7561 atcgccgtgt ggagctcccg ctccgccccc tcccagtccc ccgtggcgaa cagcacgctc
     7621 ccgtagaggg tccggcagaa ggcgaacagg aagggacacc cgaagcgctc cgtgaaccga
     7681 tcggcggctc gcacccagtg gacggcccgc tcgaactccg cgcagcgcgt gcacgagccg
     7741 atcatgtgac agctcgtgaa cacgacggtg tcgcggctgc cgccctcgcc gcccagcgag
     7801 ccggccatcg cctcatcgag gaggaccacg ccctcctcca ccttcccctg gtcgatgagg
     7861 gaggctccga cctggctcag cgcgcagagc tccagatcga ggtcgccgga cgcggcggcg
     7921 agctcccgtg cctcccgggc cttggcctcc cccagtctcg ggtcgtcgcc gtcgtatgcc
     7981 tcgaggagga ggatccagcc acggagcggc tcgagctcga actcctcgac cagtcgggcc
     8041 gcgcgcgaac gccatccgcc ggaggccgtg gggttgccga agttcgcccg atgatccacg
     8101 cacaggcgca ccgcgaccag tgcggcgctc acggggtcgg ggcggcgtcg gaacgctgcg
     8161 taggcacgct cccggaagga caccgcgtcc tggatctccc ccagccacca cagagcgtcg
     8221 gcgagcccaa ccagcccctc ggccgtctcc ccgtgctcga gcgccgaccg gaacgagtcc
     8281 cgcgccgagg cccagtctcc tgaagcgagc gcctcgttcc ccgccgcgaa caggtcgtcg
     8341 acctggttcg ccacggcccg atcctacacg ggcccggcgg aggcgccggg tccatcccga
     8401 ccgcggaacg tcacgacgcc ccacaagcga ttcgcgacgt cccccgtgat gaggaagacg
     8461 ctggtccctg agtctcgaac gcgcccgtgg cgctccaggt ggcggtgacg gatggatcga
     8521 ggttggagaa gctgataccc gcgggcagca gtctgcgcat cgctctcctc gttcgtgcgg
     8581 ccacggccgc gtgggctata gagagatgcc cgggctcccc gctccgtagc ggggccctcg
     8641 agcgcggtca gtcggccacg gtcagcgggc tgaggagcca taggcgaggc ggatccgccg
     8701 tgtagcacac gacgccgtag tcaccggggc tgagcgccct cgtccaggac tcccgctgcc
     8761 cgggcgaaga acggaaggcc tcttgtgagg cggcccactc gggagccacc cgcggatcct
     8821 cctgggcatc ctcgacgtac gccgcgagat cgtcgaagtc cctgccctcg gcgaacctga
     8881 gaacgtcgag cacgacgtcg acccgtccgt cgttcgtcag cacgaaggtg acctcgtccc
     8941 gggccgaaac cctcgccggt ccgtcatgag agcagccgag gtcgccgagg cttccgtcga
     9001 acctgacccg gagggtcgct ccctggacga acgacgttcc gcccggcctc acctcacccg
     9061 tgcccgtgac tcgctcgagg acggcggccg ccccgaggcc ggagagcccc agaaccagcg
     9121 cggccgcggt gacggtccgt gcgatgcgca gcctcgttcg ccggagcgcg ggtggcgaga
     9181 gcccacttgg tggggggagc gagcccgcct tctcccgaag cagctcccgg aggctcgcct
     9241 cgacctcgtt ctcgtgacct gtcatctcat cccgtcctga cacgcgtgcg gagaacgctc
     9301 atggccctcg agagaagcga ccggacggtc ccaggccggc actcgaggat ctgggccgcc
     9361 tcatggtccg tgagaccctc gtagtagcgg agcacgacgg cgactcgttg gcggtgcggc
     9421 agtcgctgaa gctccgacca caggtcctcc cgcagatccg atgcgagggt cgggtcaccc
     9481 tcctcgatcc gatgcccctc cctctgcaga tacgctcgct ccacctgcct cctcctgaag
     9541 tgcatcttgc agaggttcaa cacggtccga cgaaggtagg catggaacgc gtccggatga
     9601 cgaaggtgaa cgagccgtcc cgctacgttg atgaacgcct cctgcaccag atcctcggcc
     9661 aggggctggt ctcccgtcac caggtaggcc aatcggtacg cgtcggagga gtggcgcgcg
     9721 tacagctcgc ccaggcgtcc ccactcggtc gcgacccacc tccgaagctc tggcgaccat
     9781 tctgcgccgg taccttctcc catcctagat gcgcgacgtc gtggaagtgt tgcaggggat
     9841 gcctcgagat ccacggccgc atcgacaggg ctgctcgtcc tgtacttcca tccgatgctc
     9901 atcctcggcc tgctcatcaa cgcgttcgtc gtggcgaccg ccggactggg atggccggag
     9961 ctcccctacg aggaggcctg agaccggtcc cacggcccga caccgggttc gccgcggtca
    10021 gcggccgagg ggtgaccgtc acgctggtcc cgcccgccgg agcaagcccg gcggacggcg
    10081 caatggcggc tggcccgggg ccgggcctcg tgtcagaatc gcccggatgg aaaccaggcc
    10141 gcgccggacc cacaggcgcc gaggactcgg cgccaccctc gtgctggtag ctctggcgct
    10201 gctcctctcg tcgtgcgccg ccggacccaa cccggcgacc gggacgggcg aggacccggc
    10261 cgggttcctc ctgggcctct ggcacgggat catcctcccc gtcacgttca tcatctccct
    10321 cttcacggac gaggtgagcg tctacgaggt cgtcaacagc gggaactggt acgacttcgg
    10381 gttcatcctc ggcgtgctga tctctctcgg aggtggcggt gcgggcgccg gcaggcggag
    10441 aggctgacgg cccggaggac gaggaggtct tcgacagccc gagcggctgg gtccgcaagc
    10501 acgtccgcga gtacgtggag accgacggcc gcaagggcca tcgctggtcg ggcgtgcaca
    10561 ccctgctgct gaccacgcgc ggcaggaagt ccgggaagct ccggcggaca gccctcatct
    10621 acgggcgcga cggagaccgc tacctggtgg tggcctcgaa ggggggcgcg gagagccatc
    10681 cctcctggta cctcaacctg tccgagcatc cggaggtccg cgtccaggtg ggatccgacc
    10741 ggttcgtggc ccgagcccgg acggccgaca ccgaggaaaa gctccgcctc tggcggatca
    10801 tgaccgagat ctggccggag tacgaccgct accaggagcg gaccgcccga gagatccctg
    10861 tggtcgtcct cgagccggtc gccgagcgcg cgtgatcgac cacatctacc tcccggtctc
    10921 cgacgtcgag cgcagcaggg ccttctacgg cgacctgctg gggacgctgg gcatcgagga
    10981 gagcgtcacg ctcggcggat cggtgggctt cggcgtggga ggcccggggg cggtctggct
    11041 ctacccggcc atgggacgaa cgggcgtgcc cgacgacccc tgcggcctgg atcccgaggc
    11101 cggcgcgtcc ctcccccgtc ttcacatcgc tttccgagct cgctcccgga tcaggtccgt
    11161 ggcttcttcg aggaggcgcg gcgcctcggc gcggaggtcg tggagccccc acgcctcttc
    11221 ccggagtacc acgccggcta cttcgccgcg ttcgtccggg acccggaccg ccacaacgtg
    11281 gaggcggtct gcgccgcccc ccaataacta gcccgggacg gagccgatag gcccctccga
    11341 gcagggaccc cctcccctgt gggactcccc gggtcgtccg tacgctggga ggcggatgga
    11401 agggggcgaa gggtcatgaa ggagcggttc accgaagagg agtggcgggg gatcatggcg
    11461 ctcccgttcg cgatcttcgg cgtcgtcgcg ggcgccgacg gcaacatcga cgagcaggag
    11521 atcggggagt tcacgaagcg catgctgaac cccacgtgga cctacaagga cgcgatgcac
    11581 caggaggtgg ccgtggccgt cgccggggac gacgtcagcg cgctgatcaa ggaagggatg
    11641 gccctcgatc cggagcctgt gaagacggcg ctgaaggaga agctgaccgc ggacgagtac
    11701 cagagcttcc tcggcagcgt cttcatcgac gagctggccg tggcgcgggc cagcgggggc
    11761 ggcatcttcc acaaggagaa gatcgcggac aaggagaccg agaagctcgt gggctttgcg
    11821 gcctacttcg gcatcgacct gccgtccgtc cagcggatct tctcctgagc ggaaggccgg
    11881 tggccaaggg atctggctga tccctcggcc accggccctc accccgcctg gaacagcgtg
    11941 agctggagcc cctcccgccc gcggtagcgg cggttgcggt cgccccacgg cgtgtccacc
    12001 gggccggcca gcggctcggc ccccgcggcc tcgaccgccc tgc
//
LOCUS       DASUTW010000027         5858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1174640, whole genome shotgun sequence.
ACCESSION   DASUTW010000027 DASUTW010000000
VERSION     DASUTW010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5858
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174640"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..141)
                     /locus_tag="VF058_01045"
     tRNA            complement(68..141)
                     /locus_tag="VF058_01045"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(105..107),aa:Ile,seq:gat)
     gene            complement(198..608)
                     /locus_tag="VF058_01050"
     CDS             complement(198..608)
                     /locus_tag="VF058_01050"
                     /inference="COORDINATES: protein motif:HMM:NF023511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HEX6206922.1"
                     /translation="MATIQQTRRTSPAPAGGPAPRRIRVAIRRVNPWSVLKFSLLFYF
                     CLMLVFVIGLAILYMVVDALGVVASLEQLLESLGFGGDAGFEVNGGFLFRTIFLIGLI
                     SVVIWSALTLFFAFLYNLISDLVGGIEVTLAERR"
     gene            complement(611..2044)
                     /locus_tag="VF058_01055"
     CDS             complement(611..2044)
                     /locus_tag="VF058_01055"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012182691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX6206923.1"
                     /translation="MQLEPLKPFVWRVPMGTVPGMRVPGIVFATEELARAIEGDNAIE
                     QVANVATLPGIVGASFAMPDIHWGYGFPIGGVAATDPEQGGVISPGGVGFDIACGVRL
                     LRSELTLADVDDLRGLVAALGDRVPRGVGERGRMRLSRSEVEKVLAEGVRYPLSQGMG
                     WQEDEELCEDFGVLEDALPDEVSDRAIERGAPQLGSLGAGNHFLEVQIVDDILDERAA
                     DDMGLFPDQVCVMLHSGSRGLGHQVCTDYLKLVDASMREEGIEVPDRQLACVPVKHEV
                     ARRYLGAMYAAANFARANRHVLAHLVRETFEAFLGRSAREMGLRLVYDVAHNLAKMEE
                     HEVEGERRLLCVHRKGATRAFGPGSLEIPEPYRGIGQPVIIPGSMGTASYVLVGTDEP
                     TAFASTCHGAGRAMSRTQAKKRMSGSELVKQLEGEGIVMAASKPRLLAEEAPYAYKDV
                     SQVVDACEGAGLSRKVARLRPVGVVKG"
     gene            complement(2087..2500)
                     /locus_tag="VF058_01060"
     CDS             complement(2087..2500)
                     /locus_tag="VF058_01060"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HEX6206924.1"
                     /translation="MTRSYELLEHTADVGILARGDTREEAFAAAGEGLAEILGSWFPG
                     RGEEHEVRIEGPDVEALLVAWLDELLYLSEASDAVFGGFEVDRVEETRLLARVRVAPR
                     AGRALEGQHVKAATYHRLRVAQEPPGWTARVFLDV"
     gene            complement(2497..2745)
                     /locus_tag="VF058_01065"
     CDS             complement(2497..2745)
                     /locus_tag="VF058_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206925.1"
                     /translation="MGAWFAAGAATAALLMALVLRTRLPAALGVALMVAASAALAWGG
                     VLLREEPSALEAVLATTALAVMGPLHVRIVLGPYGPPR"
     gene            complement(2747..5197)
                     /gene="gyrA"
                     /locus_tag="VF058_01070"
     CDS             complement(2747..5197)
                     /gene="gyrA"
                     /locus_tag="VF058_01070"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX6206926.1"
                     /translation="MATTTDRIEPRDIEEELRSSFLDYAMSVIVGRALPDVRDGLKPV
                     HRRILWAMLEDGLRPDRPTRKSATVVGSVIGRYHPHGDQAVYDALVRMAQDFSLRYPL
                     VDGQGNFGDIQGNPPGAYRYTESRLDPLAMELLRDIDKETVDFLPNFDGNHQEPIVLP
                     ARFPNLLANGAAGIAVGMATNIPPHNLGEVIDAVVAAVDQPNITPAQLMKHVKGPDFP
                     TGGMIMGRDGIKDAYLTGRGSLKMRGRVVVEEDSKGRQRLVVTEVPYQVNPARLAEKI
                     QELVNAKRLTEVPGRGVRNETSGRGGMRIVVDLNRGANPQIVLNKLYKHTQLQESFGV
                     IMLALVDGVPKVLTLAEMVRHFVEHQLEVLTRRSKYELRKAEERDHIVEGLLKALTNI
                     DEVIKIIRGAKDADDARAKLMKRFKLSEVQANHILDMPLRRLTRLERTKLEDEHKELQ
                     AKIRRLKALLKDPKKIRKLLKDELAEVREKYADERRTEIRRDDGEIDIEDLVAREDVV
                     ITVSRAGYVKRMPVTAYRRQGRGGRGVAGGKLKEEDLVAQVFTTTTRHWLLFFTNSGK
                     VYRVKAYEVPEQSRTARGIYAANLPGVSVTSGERIAAVLAIEDYDAARYAVFATKKGM
                     VKKTALKEYDSPRTGLAAIKLRKGDELIRVRLSSGRDDVVMVSRGGKAIRFKESAVRA
                     MGRQTSGVIGMRVPPKDEVITMVLARKGEDLVTITESGLGKRTPISEYPVKGRGGQGV
                     ITHKLTARTGKLAGAFSSTKDQDVFVISTSGIVIRVPGKDIRQTGRPSQGVRIMRLEP
                     KQKVAVAAPVIESEEQEA"
     gene            complement(5199..>5858)
                     /locus_tag="VF058_01075"
     CDS             complement(5199..>5858)
                     /locus_tag="VF058_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010079770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX6206927.1"
                     /translation="PEVCELFIVEGDSAAGPAIQARDPAYQAILPIRGKILNVEKARL
                     HKILENKEIQALITAIGTSIGDEFDPSRARYGKIVVLTDADVDGAHIRTLLLTFLFRH
                     MRELIEQGSIFIAQPPLYRVTVDGKPEYLFSDRELDDLRKRLNGKKDVPVLRFKGLGE
                     MNHEQLWETAMDPDQRTLLRVTLEDAAAADRIFTILMGEDVESRREFLQQNAKDAFVD
                     V"
BASE COUNT          843 a         2109 c         1935 g          971 t
ORIGIN      
        1 gcccagagga agaacctctt cttcccgctc ttcttctcct cgcccatcgt tcctcctttc
       61 gcgtgcgtgg gcctgggtgg actcgaacca ccgacctcac ccttatcagg ggtgcgctct
      121 aaccgcctga gctacaggcc ccggggttcc gggctcagcg tacttgcgcc cgctcgaaag
      181 cgtcaatacg acgcaggtca gcggcgctcc gcgagcgtga cctcgatccc ccccacgagg
      241 tccgagatca ggttgtagag gaacgcgaag aagagtgtca gcgcggacca gatcacgacg
      301 gagatgagcc cgatcaggaa gatcgtccgg aagaggaacc ccccgttcac ctcgaagccc
      361 gcatcgcctc cgaaccccag gctctccagg agctgctcaa gcgaggccac gacgcccagg
      421 gcgtccacca ccatatagag gatcgccagc ccgatcacga agacgagcat caggcagaag
      481 tagaacagca gcgagaactt cagcaccgac caggggttga cgcgcctgat cgccacccgg
      541 atccgccggg gggcgggtcc gcccgccggc gcgggcgagg tccgtctcgt ctgctggatc
      601 gtcgccatcc ctatcccttc acgacgccga cgggacggag gcgggccacc ttcctggaga
      661 ggcccgctcc ctcgcacgcg tcgacgacct ggctcacgtc tttgtacgcg tagggagcct
      721 cctcggcaag cagccggggc ttggaggccg ccatgacgat cccttcccct tcgagctgct
      781 tgacaagctc gctaccggac atccgcttct tcgcctgcgt ccggctcatc gcgcgaccgg
      841 ccccgtgaca tgtggaggcg aaggccgtgg gctcgtcggt ccccaccagc acgtaggagg
      901 cggtccccat gctcccgggg atgatcaccg gttggccgat cccccggtag ggctcgggga
      961 tctcgagact gccggggccg aaggcccgcg tggcgccctt gcggtgcacg cacaggagcc
     1021 ggcgctcccc ctccacctcg tgctcctcca tcttggccag gttgtgcgcc acgtcgtaca
     1081 cgaggcggag gcccatctcc cgggccgagc gcccgaggaa ggcctcgaac gtctcccgga
     1141 cgagatgggc caggacgtgg cggttggccc gggcgaagtt cgccgccgcg tacatcgcac
     1201 ccaggtagcg gcgggccacc tcgtgcttca cggggacgca ggcgagctgg cgatcgggga
     1261 cctcgatccc ctcctcccgc atcgaggcgt ccacgagctt caggtagtcg gtgcagacct
     1321 gatggcccag gccccgggag cccgagtgca gcatcacgca gacctggtcg gggaagaggc
     1381 ccatgtcgtc cgcggcgcgc tcgtcgagga tgtcgtccac gatctggacc tccaggaagt
     1441 ggttgccggc gccgagcgag cccagctggg gggcgccccg ctcgatggcc cgatcggaga
     1501 cctcgtccgg gagcgcgtcc tcgaggaccc cgaagtcctc gcacagctcc tcgtcctcct
     1561 gccaccccat gccctgggac agcgggtagc ggacgccttc ggccagcacc ttctccacct
     1621 cgcttcgtga gaggcgcatc cgtccccgct cccccacccc tcggggcacc cgatctccca
     1681 gcgccgccac gagcccgcgg aggtcgtcga cgtcggcgag cgtgagctcc gagcggagca
     1741 ggcgcacccc gcaggcgatg tcgaacccca cgccgcccgg cgagatcacg cctccctgct
     1801 cagggtcggt ggcggccacc ccaccgatcg ggaacccgta gccccagtgg atgtcgggca
     1861 tggcgaagga tgcccccacg atcccgggca gggtcgccac gttcgccacc tgctcgatcg
     1921 cgttgtcccc ttcgatggcg cgggccagct cctcggtggc gaacacgatg ccgggcacgc
     1981 gcatgcccgg gaccgtcccc atggggacgc gccagacgaa cggctttagg ggttccagct
     2041 gcatggacac ccggagtcta cctacccccc ggctggttcc ttcgaatcag acgtcgagga
     2101 agacccgagc ggtccacccg gggggctcct gggccacgcg gaggcggtgg tacgtggccg
     2161 ccttcacgtg ctgcccctcg agcgcgcggc cggccctcgg ggcgaccctg acgcgggcca
     2221 gcagccgcgt ctcctccacc cgatccacct cgaacccgcc gaagaccgcg tcgctcgcct
     2281 ccgagaggta gagcagctcg tccagccagg cgacgaggag ggcctccacg tcggggccct
     2341 cgatccggac ctcgtgctcc tcgccccggc ccgggaacca cgagccgagg atctccgcca
     2401 gcccctcgcc ggccgcggcg aaggcctcct ctcgggtgtc gcccctggcc aggatcccga
     2461 cgtccgcggt gtgctcgagc agctcgtagg agcgggtcac cgcggcgggc cgtacggccc
     2521 aagtacgatc cggacgtgga gcggccccat gaccgcgagc gcggtcgtgg ccaggacggc
     2581 ctcgagggcc gagggttcct cccggaggag aacgccgccc caggccaggg ccgcggaggc
     2641 ggccaccatc agggcgacgc cgagggccgc ggggagcctg gtccggagga cgagggccat
     2701 gagcagggcg gccgtggccg cgccggcggc gaaccaggcg cccacgtcag gcctcctgct
     2761 cctcgctctc gatcaccggc gcggccacgg ccaccttctg cttcggctcc aagcgcatga
     2821 tcctcacgcc ctggctcggc cggccggtct gacggatgtc cttccccgga acccggatca
     2881 cgatcccgga ggtcgagatg acgaagacgt cctggtcctt ggtggaggag aacgcccccg
     2941 ccagcttccc cgtcctcgcc gtgagcttgt gggtgatcac gccctggccc cctcgtccct
     3001 tcaccgggta ctcggagatc ggcgtgcgct tgcccagccc ggactcggtg atcgtcacga
     3061 ggtcctcgcc cttccgggcc agcaccatcg tgatgacctc gtccttcggg ggcactcgca
     3121 tcccgatcac gccgctggtc tggcggccca tcgcgcgcac cgcgctctcc ttgaaccgga
     3181 tcgccttccc cccccgggat accatcacga cgtcgtcgcg cccgctggag agccgtacgc
     3241 ggatcaactc gtcgcctttc cggagcttga tcgcggcgag gccggtccgc ggtgagtcgt
     3301 actccttgag cgcggtcttc ttgaccatcc ccttcttcgt ggcgaagacc gcgtacctgg
     3361 cggcatcgta gtcctcgatg gccaggacgg ccgcgatccg ctcgccggac gtgaccgaga
     3421 ccccggggag gttggccgcg tagatgccgc gggcggtgcg ggactgctcc gggacctcgt
     3481 aggccttcac ccggtagacc ttcccgctgt tcgtgaagaa caggagccag tgccgggtgg
     3541 tggtggtgaa gacctgggcc accaggtcct cctccttcag cttccctccg gccacgccgc
     3601 gcccgccccg accctgcctc cggtaggccg tcaccggcat ccgcttcacg tagccggccc
     3661 gggagaccgt gatgaccacg tcctctcttg ccacgaggtc ctcgatgtcg atctcgccgt
     3721 catcccgccg gatctcggtc cgtcgctcgt ccgcgtactt ctcgcggacc tcggcgagct
     3781 cgtccttgag gagcttccgg atcttcttgg ggtccttcag gagggccttg aggcgcctga
     3841 tcttggcctg cagctccttg tgctcgtcct cgagcttggt gcgctcgaga cgggtgaggc
     3901 gccgcagggg catgtccagg atgtggttcg cctgaacctc cgagagcttg aagcgcttca
     3961 tcagcttcgc ccgcgcgtcg tcggcgtcct tcgccccccg gatgatcttg atgacctcgt
     4021 cgatgttcgt gagggccttc aggagcccct ccacgatgtg gtcccgctcc tcggccttcc
     4081 ggagctcgta cttcgagcgg cgggtcagca cctcgagctg gtgctcgacg aagtggcgga
     4141 ccatctccgc gagggtcagg accttgggga ccccgtccac cagggcgagc atgatcacgc
     4201 cgaagctctc ctggagctgc gtgtgcttgt agagcttatt gaggacgatc tgggggttgg
     4261 cgccgcggtt gaggtccacg acgatccgca tgccgccccg gcccgaggtc tcgttgcgca
     4321 cgccgcggcc gggcacctcg gtgagccgct tggcgttcac cagctcctgg atcttctccg
     4381 ccagcctggc cgggttcacc tggtagggca cctcggtgac cacgaggcgc tggcggccct
     4441 tggagtcctc ctccacgacc acccggcccc gcatcttcag cgagccgcgg ccggtgaggt
     4501 aggcgtcctt gatcccgtcc ctccccatga tcatgccgcc cgtggggaag tccggtccct
     4561 tgacgtgctt catcagctgg gccggcgtga tgttgggctg gtccacggcg gccaccacgg
     4621 cgtcgatcac ctcgcccagg ttgtggggag ggatgttcgt ggccatgccc acggcgatcc
     4681 ccgccgcgcc gttggccagg aggttaggga accgcgccgg aagcacgatc ggctcctggt
     4741 ggttcccgtc gaagttcggg aggaagtcga cggtttcctt gtcgatgtcc cgcagcagct
     4801 ccatcgccag cgggtcgagg cgcgactccg tgtaccggta cgccccgggg ggattcccct
     4861 ggatgtcgcc gaagttcccc tgtccgtcca caagcggata ccggagcgag aaatcctgtg
     4921 ccatgcggac gagtgcgtcg tagacggcct ggtcgccgtg ggggtggtag cggccgatca
     4981 cggatcccac gacggtggcc gacttgcgcg tcggacggtc ggggcgcaga ccgtcctcga
     5041 gcatggccca gaggatccgc cggtggacgg gcttcaggcc gtcgcggacg tcggggaggg
     5101 cccgcccgac gatgaccgac atcgcgtagt ccaggaagga gcttcggagc tcttcctcga
     5161 tgtcgcgggg ctcgatgcgg tcggtggtcg tcgccatctc agacgtcgac gaaggcgtcc
     5221 ttcgcgttct gctggaggaa ctctcggcgc gactctacgt cctcccccat caggatcgtg
     5281 aagatccgat cggcggccgc ggcgtcctcg agggtcaccc gaaggagggt ccgctgatcg
     5341 ggatccatcg cggtctccca cagctgctcg tggttcatct cgcccagccc cttgaaccgc
     5401 aggaccggga cgtccttctt cccgttcagc cgcttgcgca ggtcgtcgag ctcgcggtcg
     5461 ctgaagaggt actcgggctt gccgtccacc gtcacccggt agagcggcgg ctgagcgatg
     5521 aagatgctcc cctgctcgat cagctcgcgc atgtgacgga agaggaaggt cagaagcagc
     5581 gtccggatgt gcgccccgtc cacgtcggcg tcggtgagca cgacgatctt cccgtagcgc
     5641 gctctcgagg ggtcgaactc gtccccgatg ctggtcccga tcgccgtgat cagagcctgg
     5701 atctccttgt tctccaggat cttgtggagg cgggccttct ccacgttgag gatcttcccg
     5761 cggatgggga ggatcgcctg gtaggcgggg tccctggcct ggatcgccgg cccggccgcc
     5821 gagtccccct ccacgatgaa cagctcgcac acctcggg
//
LOCUS       DASUTW010000028         3164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1175265, whole genome shotgun sequence.
ACCESSION   DASUTW010000028 DASUTW010000000
VERSION     DASUTW010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175265"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..834)
                     /locus_tag="VF058_01080"
     CDS             complement(124..834)
                     /locus_tag="VF058_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206928.1"
                     /translation="MSREFIEGVLIVVSGFVVFGGSVWLLLAAILGVRMGYLVAATGF
                     FAFLLILSALWTFGAPIPGGEVPAYLGPKGELPHWVPVGAGVQLTSETFPEIERFPGG
                     PWDDPADDPAFADEVEPATGAFQEFLAERAAEELGRQGIEGEVAPEAFEVEQLRFATA
                     EDGTELAAARAFATTGGPEVVALAYKNEGNEPLPSWLFLGASVIGLAVHLPFLDRAER
                     RRKEFLTGGDQAPWRGPA"
     gene            complement(834..1553)
                     /locus_tag="VF058_01085"
     CDS             complement(834..1553)
                     /locus_tag="VF058_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6206929.1"
                     /translation="MGVVALSGLAITLIVLGVLVALAFVAALVWLGLRGRPERGPDIP
                     AGMRPGPPDEMLERRFVDRFLGWSFLFVIFFAVWLAGLWLMEPQTNADDAVTLTERSV
                     ERGSMWFAISDEANPTGFGCARCHGPAGTGGQEIPFGDGFVQPPALNDVCGGPETGHP
                     LIESINDVRTTIEQGRPGTAMPSWSIRYEGAMNDQQIDDLLNYLLTIQELPDGAQNVC
                     LEPLEGAEDGTGGNGGEEGGA"
     gene            complement(1554..2273)
                     /locus_tag="VF058_01090"
     CDS             complement(1554..2273)
                     /locus_tag="VF058_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX6206930.1"
                     /translation="MSRETIAIIIVAVLFGQFLILLVASLIRSRAAARRAEEREAPAE
                     EAKEERAPAPVSRRDFFRRSLLASLGLFAAQFGAASLAFLWPTLREGFGAVIDLGISA
                     DEILGAIQDDRQPFYYGAGRFYLVAYDASQDPDGMYEGLVDGGLMALYQKCVHLGCRV
                     PFCEQSQWFECPCHGSKYNRAGEYRDGPAPRGLDRFAVRIDNGNVTVDTSSIIEGPAR
                     GEVTIQAEPEGPFCINITAEE"
     gene            complement(2270..3100)
                     /locus_tag="VF058_01095"
     CDS             complement(2270..3100)
                     /locus_tag="VF058_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HEX6206931.1"
                     /translation="MAERAEELNKETFERVRDELLQQGLPPRVAEGKARRIAMIEYNK
                     EHGAEPSRPPKEAAPGEAPAQPEGAPAAPAPQQAPQTPESGGVAAPPAAALRTAPAPT
                     GKPPVPKPGAPEKHRLLALVPPEGVQRVDREQGDRINVFPHLLLEELLALIIVTAALI
                     VFSTFVNAPLRELANQNLTPNPSKAPWYFLGLQELLRYFHPMVAGVTIPTVVFAFLFV
                     LPYVDRNPSNKPGDRKMAIVLWTIFLMFWTILTMIGAFFRGPGFNFVWPWVEGIFFEL
                     "
BASE COUNT          492 a         1119 c         1127 g          426 t
ORIGIN      
        1 gcgcgccatc gagatgagga acacgacgaa gatcaccacg agcatgaggg cgaagaggat
       61 gaacgagagc ccgccctcct cgatcgccag cacgatggtg gtcacgtcca cgtccatctc
      121 ctcctaggca gggccccgcc acggggcctg gtcgccgccg gtcaggaact ccttccgcct
      181 ccgctcggcc cgatccagga acggcaggtg gaccgccagc ccgatcacgg acgccccgag
      241 gaacagccac gacgggagcg gctcgttacc ctcgttcttg tacgccagcg ccacgacctc
      301 cggaccgccc gtggtggcga atgcccgggc cgcggcgagc tcggtgccgt cctcggccgt
      361 ggcgaagcgc agctgctcga cctcgaaggc ctcgggagcg acctcgccct cgatgccctg
      421 ccgtcccagc tcctccgcgg cccgctcggc caggaactcc tggaaggcgc cggtcgcggg
      481 ctcgacctcg tcggcgaagg ccgggtcgtc ggccggatcg tcccacggcc cgccggggaa
      541 ccgctcgatc tcggggaagg tctcggaggt gagctgcacg cccgccccta ccgggaccca
      601 gtgcggcagc tcgcccttgg gcccgaggta ggcggggacc tctccgccgg ggatcggagc
      661 gccgaaggtc cagagggcgg agaggatcag gaggaaggcg aagaagccgg tggcggccac
      721 gagatagccc attcgcacgc ccaggatcgc ggccagcagc agccacaccg accctccgaa
      781 gaccacgaag cccgacacga cgatgaggac cccctcgatg aactcgcggc tcatcaggcg
      841 cccccctcct cgccgccgtt cccgccggtg ccgtcctcgg ccccctccag cggttccagg
      901 cacacgttct gggcgccgtc cgggagctcc tggatcgtga ggaggtagtt caagaggtcg
      961 tcgatctgct ggtcgttcat ggcgccctcg tagcggatgc tccacgaggg catcgccgtc
     1021 cccggacggc cctgctcgat cgtcgtcctg acgtcgttga tggactcgat cagcgggtgg
     1081 cccgtctcgg ggccgccgca gacgtcattg agggcaggcg gctgcacgaa cccgtcgccg
     1141 aacgggatct cctggccccc cgtcccggcc gggccgtggc accgcgcgca gccgaagccg
     1201 gtggggttcg cctcatcgga gatggcgaac cacatcgacc cccgctccac ggagcgctcg
     1261 gtgagcgtga cggcgtcgtc ggcgttcgtc tggggctcca tcagccagag gccggccagc
     1321 cacacggcga agaagatcac gaagaggaag gaccagccca ggaagcggtc cacgaagcgc
     1381 cgctccagca tctcgtcggg ggggccgggg cgcatccccg ccgggatgtc tggcccgcgc
     1441 tcggggcgcc cgcggagacc cagccacacg agagcggcca cgaaggccag cgccacgagc
     1501 acgcccagga cgatgagggt gatcgccagg ccggagagcg cgacgacccc cacctactcc
     1561 tccgccgtga tgttgatgca gaacgggccc tcgggttcgg cctggatcgt cacctcaccc
     1621 cgggccgggc cctcgatgat cgagctcgtg tccacggtca cgttgccgtt gtcgatccgg
     1681 acggcgaagc gatcgaggcc ccggggggcg gggccgtcgc ggtactcgcc ggcccggttg
     1741 tacttcgagc cgtggcatgg gcactcgaac cactggctct gctcgcagaa cggcacccgg
     1801 catccgaggt gcacgcactt ctggtagagc gccatcaggc ccccgtccac caggccctcg
     1861 tacatcccgt cggggtcctg cgaggcgtcg taggccacga gatagaagcg gcccgcgccg
     1921 tagtagaagg gctggcggtc gtcctggatc gcgcccagga tctcgtcggc gctgatgccg
     1981 aggtcaatca ccgccccgaa cccctcgcgg agggtcggcc agaggaaggc caggctggcg
     2041 gcgccgaact gcgcggcgaa caggcccagc gaagccagca gcgagcgccg gaagaagtcc
     2101 cggcgcgaga ccggcgccgg cgcgcgctcc tccttggcct cctcggcggg ggcctcgcgc
     2161 tcctcggccc gacgggcggc ggcccgcgac cggatcaggg aagccacgag caggatcagg
     2221 aactgcccga acaggaccgc gacgatgatg atggcgatgg tttctctgct catagctcga
     2281 agaagatgcc ctcgacccag ggccacacga agttgaaccc cggccctcgg aagaacgcgc
     2341 cgatcatggt caggatcgtc cagaacatga ggaagatcgt ccacagcacg atcgccatct
     2401 tgcggtcccc gggcttgttg ctggggttgc ggtccacgta ggggaggacg aacaggaacg
     2461 cgaacacgac cgtggggatc gtcacgccgg ccaccatggg gtggaagtac cgcagcagct
     2521 cctgcagacc caggaagtac cagggggcct tggacgggtt cggcgtcagg ttctggttgg
     2581 ccagctcccg gagcggcgcg ttcacgaacg tggagaagac gatcagcgcc gcggtcacga
     2641 tgatcagcgc cagcagctcc tcgaggagca ggtgggggaa gacgttgatc cggtcgccct
     2701 gctcgcggtc cacgcgctgg accccctcgg gcggaacgag cgccaggagg cggtgcttct
     2761 ccggcgcgcc cggcttgggg accggcggct tgccggtggg ggcgggcgcg gtgcgcaggg
     2821 ccgcggcggg tggggcggcc acccccccgg actcgggcgt ctggggcgcc tgctggggcg
     2881 ccggggccgc gggggcgccc tcgggctggg ccggcgcctc gccgggggcg gcctccttcg
     2941 gaggccggga gggctcggcg ccgtgctcct tgttgtactc gatcatcgcg atgcgccggg
     3001 ccttcccctc ggcgacgcgc gggggcaggc cctgctggag cagctcgtcg cggacccgct
     3061 cgaaggtctc cttgttgagc tcttcagctc gctcggccat cccgcgctcc tagaggggtc
     3121 cggagatccc gccgtccttg cggatccgcc agaagtggat cgcc
//
LOCUS       DASUTW010000029         4673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_916732, whole genome shotgun sequence.
ACCESSION   DASUTW010000029 DASUTW010000000
VERSION     DASUTW010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916732"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1805)
                     /locus_tag="VF058_01100"
     CDS             complement(<1..1805)
                     /locus_tag="VF058_01100"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding domain-containing
                     protein"
                     /protein_id="HEX6206932.1"
                     /translation="MTDQPPRAEAGGVELPEGTVTVLFTDVEGSTDLRTREGDDVAQE
                     LLRAHEHLLREQVEAHGGREVVFMGDGFMIAFGSTRSALRCAVGIQRAFDEHNRAHPN
                     RPIRVRAGLNAGEVRQEGGTLYGTAVNAAARIMGKADGGQVFVSQIVRDLTAGVRDFA
                     IVDRGLHDLKGFPEPWRLYEVRWEEEPADEAETAAPVPSTPLAQNAIEGTVPRSAFEL
                     VGRHAERQVLTEALEEAAEGRVRIVSLEGEAGIGKTRLIEVAARSAAERGFGVLSAGG
                     DEELRGPFLLLRTLLSSTAMEALADRCHSRQSLERARRVLAGEGHEGHEGLTPSEQML
                     HVYDVATVALRGIAAEQPVAVLLDDLQWADEDSLKLVRYLVRTSAGLPIFVLMAIRWE
                     VEPTQTPASALIADLERMGVARRMTLERFSRSESEDLLAQILGAPVSREAGATLHERG
                     EGVPFFLVEFARAYREARLLQQVRGRWEIGGDPRQTVPTSVQILLERRLAQLPEETRR
                     VLGDAAVVGRRIRLDDLAEVRRRAGDAEATPISLDEQLTPAVAHSMLAELPEGPHDYA
                     FTHDQIRSVLLESQSKARRRAVHAALQEIVAERAGV"
     gene            complement(1830..3242)
                     /locus_tag="VF058_01105"
     CDS             complement(1830..3242)
                     /locus_tag="VF058_01105"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006911980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX6206933.1"
                     /translation="MPVGSIAPELIVLGGSVGVLLWALFAPRRLQASGAAVLSLAVIA
                     AAGIAAWPMLRGGQGTTFFGTYAVDDTAVWAKLLILGVTAVTVLLSYEWFRTEPRHGE
                     YYSLLLMSALGAILLAGAADLMEVLLGVLLASATGYVLAGYHRAHAPSGEAAIKYFLL
                     GAFANAAMVFGVALLFGLGGSTLLGVLRDELVGTSSVGLVGGAVLVLVGLLFKMGAVP
                     AHAWMPDVAEGSPAPVAAFVTVAPKIGALIAVGRLMLALPEDGLGWRPLVAVVAALTM
                     TLGNLTALWQDDVRRLLGWSAVSQSGYGLMAVVALGRSDLAVPSLLFFLAAYALANLA
                     AFGVVIELRGRTDRLRYAGLGRTHPALALSLVVAFLSFVGIPPLAGFTAKLALFGATI
                     EAGYTWLAVLAILNTVVSLAYYARVLAPAYFEPVAEPLPVLGRWARTATLATAAGVVV
                     LGILAQPFLDGFSLAGLFPG"
     gene            complement(3242..>4673)
                     /locus_tag="VF058_01110"
     CDS             complement(3242..>4673)
                     /locus_tag="VF058_01110"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX6206934.1"
                     /translation="ALLPASRERLLRAIAIAAAGFPLVPLTVTWARMEGGEIQLVEAF
                     DWIPTLGVGYRVGVDGVSLTLAALTALLFVVSLIFPEDLRGRPSAYFALFLFLETASL
                     GVFLAMDLFLFYVFWDLSLAGMYFVIGGWGHGDRQRSALKFFLYTLAGSLAMLLAILG
                     LYLATEPRTFDMVEIVRQQPVAGTGLRASLIFLGFAIGFAIKAPLVPFHTWLPPAHVD
                     APAPGSTILAGVLLKMGTYGFIRILMPMLPEAFRAYALALAILGVVSIVYGALVTLAQ
                     RNVKRLIAYSSVTHMGYTMLGIAAAGALATGEVEARSLALTGATVEMVAHGLITGGLF
                     LIAGTLWRRGETYDLDALGGLASRAGRATAATVVLAFASLGLPGLAGFVAEFQIFVGT
                     FSVYPWLAGIGLLGVILLAAAFLRMLREAFLGPLPEELRDWTDLDAPETVSLAPLLLL
                     TVVIGIAPAWLLDVIDRTARALLGGG"
BASE COUNT          767 a         1730 c         1643 g          533 t
ORIGIN      
        1 acgccggccc gctcggcgac gatctcctgc agggcggcgt ggaccgccct gcgccgcgcc
       61 ttgctctggg actcgaggag caccgagcgg atctggtcgt gggtgaacgc gtagtcgtgc
      121 ggtccctcgg gcagctccgc cagcatgctg tgggccaccg cgggcgtgag ctgctcgtcg
      181 aggctgatcg gcgtcgcctc cgcatcgccc gctcggcggc ggacctccgc cagatcgtcc
      241 aggcggatcc ggcggccgac caccgcggca tcgcccagca cccggcgggt ctcctcgggg
      301 agctgagcca ggcgtcgctc caggaggatc tgcacgctgg tcggcacggt ctggcggggg
      361 tcgcccccga tctcccagcg cccccgcacc tgctggagca ggcgagcctc ccggtaggcg
      421 cgagcgaact ccaccaggaa gaagggcaca ccctcccccc gctcgtgcag ggtggcgccc
      481 gcctcccgac tcaccggagc gccgaggatc tgcgccagca ggtcctccga ctccgaacgg
      541 gagaagcgct ccagggtcat ccggcgcgcc acgcccatgc gctcgaggtc ggcgatcagg
      601 gcgctggccg gggtctgcgt gggctccacc tcccaccgga tcgccatcag gacgaagatg
      661 ggcaggccgg ccgacgtgcg aacgaggtat cggacgagct tcagggagtc ctcgtcggcc
      721 cactggaggt cgtcgagcag gacggccacg ggctgttccg cggcgatccc ccggagcgcg
      781 accgtcgcga catcgtagac gtggagcatc tgctcggagg gggtgagccc ctcgtgcccc
      841 tcatgccctt cgccggccag gacgcgacgg gcccgctcca gcgactgccg actgtggcac
      901 cggtcggcga gcgcctccat cgccgtggag ctgagcaggg tccgcagcag aaggaagggc
      961 ccgcggagct cctcgtcgcc cccggccgac agcacgccga agcctcgctc cgcagcggac
     1021 cgggccgcga cctcgatcag cctcgtcttg ccgatccccg cctcgccctc gagcgagacg
     1081 atcctcacgc gaccctccgc cgcctcttcc agcgcctccg tcagcacctg tctctccgcg
     1141 tggcggccca ccagctcgaa cgcggatcgc ggaacggtcc cctcgatcgc gttctgggcg
     1201 agcggtgtcg aggggaccgg cgccgccgtc tcggcctcgt ccgcgggctc ctcctcccac
     1261 cggacctcgt acagccgcca cggctcgggg aaacccttga ggtcgtgcag cccccgatcg
     1321 acgatggcga agtctcggac ccccgcggtg aggtccctca cgatctgcga gacgaacacc
     1381 tggcccccgt cggccttccc catgatccgt gcggccgcgt tcacggcggt cccgtagagc
     1441 gtcccgccct cctgccgcac ctcgccggcg ttcaagcccg cccgcaccct gatcggccgg
     1501 ttggggtgtg cgcggttgtg ctcgtcgaac gctcgctgga tgcccacggc gcaccgcagg
     1561 gcgctccgcg tcgagccgaa cgcgatcatg aacccgtccc ccatgaagac gacctcgcga
     1621 ccgccgtgcg cctcgacctg ctcgcggagg agatgctcgt gcgcccgaag cagctcctgg
     1681 gcgacgtcgt cgccctcgcg ggtgcggaga tcggtggatc cttcgacgtc ggtgaacagt
     1741 accgtgacgg tgccttcggg gagctcgaca ccgcccgcct ctgctcgggg tggctggtcg
     1801 gtcacgaccg cagggtatcg cgatggcgtt cagccgggaa acagaccggc cagcgagaag
     1861 ccgtccagga acggctgggc caggatcccc aggaccacga cgccggcggc ggtggccagt
     1921 gtggccgttc gagcccaccg ccccagcacg gggagcggtt cggcgaccgg ctcgaagtac
     1981 gcgggcgcca gcaccctcgc gtagtacgcg agggacacga ccgtgttgag gatcgccagc
     2041 acggccagcc acgtgtagcc ggcctcgatc gtcgcgccga acagcgccag cttggcggtg
     2101 aagcccgcca gcggcgggat ccccacgaac gagaggaacg ccacgacgag cgacagggcg
     2161 agagccgggt gggtgcggcc caggcccgcg taccggaggc ggtcggtgcg gccccggagc
     2221 tcgatcacga cgccgaacgc cgccaggttc gccagcgcgt aggccgccag gaagaagagg
     2281 agggagggaa ccgcgaggtc gctcctcccc agcgccacca ccgccatcag cccgtagccc
     2341 gactgggaga cggccgacca gcccagcagg cgacgcacgt cgtcctgcca cagggcggtg
     2401 aggttcccca gggtcatcgt gagggcggcc acgacggcga ccaggggccg ccagccgagg
     2461 ccgtcctcgg ggagggcgag catcaggcga cccaccgcga tcagcgcccc gatcttgggg
     2521 gccacggtga cgaacgcggc caccggcgca ggcgagcctt cggccacgtc gggcatccac
     2581 gcgtgcgccg ggaccgcccc catcttgaac agcaggccga ccaggacgag cacggcgccg
     2641 ccgacgagtc ccaccgatga ggtgccgacg agctcgtcgc ggagcacccc cagcagcgtg
     2701 gagccgccga gcccgaacag cagcgccacg ccgaacacca tcgcggcgtt ggcgaacgcc
     2761 cccagcagga agtacttgat cgcggcctcg cccgagggcg cgtgggcccg gtggtaaccg
     2821 gccagcacgt acccggtggc cgaggccagc aggaccccga gcaggacctc catcaggtcc
     2881 gccgcgcccg ccagcaggat cgccccaagc gccgacatca gaaggaggga gtagtactcg
     2941 ccgtggcggg gctcggtccg gaaccactcg tacgacagca gcacggtcac ggccgtgacc
     3001 ccgaggatca gcagcttggc ccagaccgcg gtgtcgtcca ccgcgtaggt cccgaagaac
     3061 gtcgttccct gtcccccgcg cagcatcggc caggcggcga tcccagcggc cgcgatcacg
     3121 gcgagcgaga ggaccgcggc ccccgaggcc tggagacggc ggggcgcgaa gagcgcccac
     3181 agcagcacgc cgaccgaccc gccgaggacg atcagctcgg gcgcgatcga cccgaccggc
     3241 actagccgcc tccgagcagc gcgcgggccg tccggtcgat cacgtcgagg agccaggcgg
     3301 gggcgatccc gatgaccacg gtgaggagca ggagcggggc caacgagacc gtctcgggag
     3361 cgtcgaggtc ggtccagtcc cggagctcct cggggagagg ccccaggaac gcctcgcgga
     3421 gcatccgcag aaacgcggcc gccagcagga tcaccccgag gaggccgatc cccgccagcc
     3481 acgggtacac ggagaaggtc cccacgaaga tctggaactc ggccacgaac ccggccaggc
     3541 ccgggagccc caggctggcg aaggccagga cgacggtcgc ggcggtggcg cggccggccc
     3601 gcgacgcgag cccgcccagc gcgtcgaggt cgtaggtctc gccccgccgc cacagcgtcc
     3661 ccgcgatcag gaacagcccg ccggtgatca gcccgtgcgc caccatctcc accgtggcgc
     3721 cggtcagcgc cagcgaccgc gcctcaacct cgcccgtggc gagcgcgccc gcggccgcga
     3781 tgcccagcat cgtgtagccc atgtgggtga cgctcgagta cgcgatcagc cgcttgacgt
     3841 ttcgctgggc cagggtcacc agggccccgt agacgatcga gaccacgccg aggatcgcca
     3901 gcgccagcgc gtacgcgcgg aaggcctccg ggagcatcgg catcaggatc ctgatgaacc
     3961 cgtaggtccc catcttcagg aggacgccgg ccaggatcgt cgagccgggg gccggcgcgt
     4021 ccacgtgcgc gggcggcagc caggtgtgga acggcacgag cggcgccttg atcgcgaacc
     4081 cgatcgcgaa cccgaggaag atcagcgagg cgcggagccc cgtcccggcc accggctgct
     4141 gacgaacgat ctccaccatg tcgaaggtcc gaggctcggt ggccaggtac agccccagga
     4201 tcgccagcag catcgccagc gagccggcca gcgtgtagag gaagaacttc agcgccgacc
     4261 gctggcgatc gccgtggccc catcccccga tcacgaagta catcccggcc aggctcaggt
     4321 cccagaacac gtagaagagg aagaggtcca tcgccaggaa gaccccgagc gaggccgtct
     4381 ccaggaagag gaagagcgcg aagtacgcac tgggcctccc gcggaggtcc tcggggaaga
     4441 tcagcgagac gacgaacagg agcgccgtca gggcggccag cgtgagcgac accccatcca
     4501 cccccacccg gtagccgacg cccagtgtgg ggatccagtc gaaggcctcc acgagctgga
     4561 tctcgccgcc ttccatgcgg gcccaggtga cggtcagcgg gacgaggggg aagccggcgg
     4621 cggcgatcgc gatcgcgcgg aggagtcgct cgcggctcgc gggcagcagc gcg
//
LOCUS       DASUTW010000030         7219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1823575, whole genome shotgun sequence.
ACCESSION   DASUTW010000030 DASUTW010000000
VERSION     DASUTW010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7219
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823575"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1586)
                     /locus_tag="VF058_01115"
     CDS             complement(<1..1586)
                     /locus_tag="VF058_01115"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6206935.1"
                     /translation="MSPARALLVALSLVAAACSAGAPAARRSPEPSGGSPSVAPIPST
                     RMEEVACGLPAPWILRIARGYHPERSAELQILPREPNFVGAGLPHVGPWTYTSHVPMF
                     WYGPGHVEPQGRVRRAATLADIAPTHAQMLGLDFPTPDGKPLTEALVEDPGGPPKLIV
                     TVIWDGAGRSLLDEWRGRWPNLRRLIPEGAWYEEAFAGSSPTSSAQGHGIIGTGTFPR
                     THGLVGHSIRIGDRIVPPWRTGPDLLLRPSLADLWMQETGGRAKAGLLGTVGIQLGML
                     GHGSAWEGNPKPLVVLREAPNAETLGAEGTRWNLPEHLLEFYRFPGYANDLPELAGYF
                     PDIDRRDGQADGTWRGHALDDEVIQGGFQTPARIPYQTRLLEEVIEREGFGEDDVTDL
                     LFVNYKLIDQLGHIFTMNSEEMGDAVEVQDEHLPVLIDILNRLVGEDEWAMVITADHG
                     STPSPEASGAFQISATELHQRLNGAFDGDDDDRPVVQQVKQTEIFVDTEELAEHGGTL
                     EEMAEFVMDLRQEDTAIPDVS"
     gene            1650..2003
                     /locus_tag="VF058_01120"
     CDS             1650..2003
                     /locus_tag="VF058_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206936.1"
                     /translation="MTERARDALRRELYWGDEPPAAGPTRELRARIERLADSLQADLA
                     RPFPADPSFSGGSRWRLGVKRFLYRLNRPLTRRHDRVAAELAAISLELLDHLGRVEAE
                     LRRIEERLPEEGEAP"
     gene            2000..3049
                     /locus_tag="VF058_01125"
     CDS             2000..3049
                     /locus_tag="VF058_01125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6206937.1"
                     /translation="MRIAVCHPQTPFTRGGAEMHMEGLVAALRADGHDVDVVTMPFKW
                     YPPSEIVHQMASWRSVDLSEANGQRIDMVIALKFPAYLVAHERKVVWLIHQHRTAYEL
                     WDHPEYGDLRLHEDGDAVRDMIHRADRAGLAEAKRIFTNSANVRDRLRRSVGLSADVL
                     YHRSPLCEALLEVEPGPHGDHVLLPSRLEPLKRQSLAIDAMRHVRSGARLVLVGKGPG
                     EDALRRRVAEAGLEDRVELAGPVPDEELIELYRTALAVFYGPYDEDYGYVTIEAMAAS
                     RPLVTCTDSGGPLELVHDGEDGRVVAPEPKAIAGAIDALHQGRDAAARMGAAGRSLVE
                     ARVPPWPEVARRLLS"
     gene            3046..4311
                     /locus_tag="VF058_01130"
     CDS             3046..4311
                     /locus_tag="VF058_01130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6206938.1"
                     /translation="MKVAFLSPLPPAPTGIAAYSRAVLDRLERRGIGLELMPRAPRSL
                     EEAERLAETSDLAVYHVGNNPLFHRHIYGAAIRRPGLVVLHDLALDDLIRGLDAASDP
                     TAGPARAEARAAARALRTSGLDVDPPLATAWSAHLVRRARGVIVHAPFGARYLEAFGC
                     RTPVHVVPHPPVPEPSGPRAARADRRVRRRLAGRGAVVGVLGDIGAAKGIEAVLDAVA
                     RLGQPAVLAIVGRRIPGFDVAGAVGRSGLGDRVLVDQDVSDAVFGAWLRACDVVVNLR
                     HPHRGEVSGTLVRAMQAGVPTVVSATGTYLDLPPDAVVRVPAGPPDPEALVEALGVLL
                     AHPARRRAIGERAREHAERLDREDATAEGYARAIEETLRIVRDPRRPAVARWAAALRD
                     VGAGDEVIERGLGSEYVDALTSLDPDATT"
     gene            complement(4301..5680)
                     /locus_tag="VF058_01135"
     CDS             complement(4301..5680)
                     /locus_tag="VF058_01135"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6206939.1"
                     /translation="MDSFIAHRRFSRRDFLRLSAGSAAGALLASCTSGVRPTFGQNLD
                     DVDTRWPIKRVVYIMLENRSFDHMFGTFPGVNGVTVGVDLGRERPLTPAPEWLAGDIP
                     HDRSAAIKCVNDGQMDNFALGELGPYFAYTQFSAQNLPIYWNWAKDYVLCDNFFASVN
                     GPSYPNHLFFVAGTNGGAIDNPENIRVFKDGDKWIKSWGIDAYGDDVFVLVEDPDGNV
                     TKHSTTFHLPTMGEQLTERDIDWAYYSPEPHQSGYMWNAYAAIPDVFHTELWDEHIWP
                     VDEILVDIEANALPPVTWVVPRFQLSDHPPFSTCHAMNWVNDIVSGIMRSDMWEHTAI
                     FVTWDEWGGFYDHVVPPVIGERELGVRVPMFVISPYARKGYVDDAEGEFTSPLKFIAD
                     NWDLPYLTDAIADTHNFEHVFDFDRPPRRPTPQPAAGHCQGEPLEYIGDLPEWPPGVE
                     GRSPGDIRS"
     gene            5744..6418
                     /locus_tag="VF058_01140"
     CDS             5744..6418
                     /locus_tag="VF058_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206940.1"
                     /translation="MMGVSRVSKRWIGGLAAVLILVACGGDQASDTRQEEPSLPTEVS
                     TAEETPPPPREPDGFEPESGEAGDTCVNGWNRPLRDDPIVLEGLRIIRRHMGVDGGFS
                     LQDFRYFEGPESPPSDKGYLLTVRRWYYKGHLKSDPGFRGRWLVEWREFGPGIAAVAP
                     YDTEGFRSPDWVGFEYRERLEPRSFEGIPGEWRGEPFDFVRGAREPDIRVEGLPAEVR
                     GCLAGT"
     gene            6473..>7219
                     /locus_tag="VF058_01145"
     CDS             6473..>7219
                     /locus_tag="VF058_01145"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX6206941.1"
                     /translation="MFESLGVRMGFVHLHCRSAFSLKDGAFLPEALAAAAAELGMPAV
                     ALTDRDALYGAARFVDACERVGVRPILGSLLTLDEGEVLLLARTDAGYANLCRLITHA
                     HMSGERGEPSVTAGEVLRRADGLVALLGPGSPPGRLALRGRLGAARELLRPWREAFGG
                     WCFVEVRNLLEPMSAAEVRTLLRLADEAGVPAAATNAVRHLVPEDAFVADALECMREL
                     VPVAEHHLTRWNNEGFLKPAAAMRGLFAGRP"
BASE COUNT          940 a         2566 c         2658 g         1055 t
ORIGIN      
        1 gagacgtcgg ggatcgccgt gtcctcctgc cgcaggtcca tcacgaactc ggccatctcc
       61 tcgagcgtcc ccccgtgctc cgccagctcc tcggtgtcca cgaagatctc ggtctgcttc
      121 acctgctgga ccacggggcg gtcgtcgtcg tccccgtcga aggccccgtt gaggcgctgg
      181 tggagctcgg tggcggagat ctggaacgct ccgctcgcct cagggctcgg ggtcgagccg
      241 tggtccgcgg tgatcaccat ggcccactcg tcctcgccca cgagacggtt cagaatgtcg
      301 atcaggacag ggaggtgctc gtcctgcacc tcgaccgcgt cgcccatctc ctcgctgttc
      361 atcgtgaaga tgtggcccag ctggtcgatc agcttgtagt tcacgaagag caggtcggtg
      421 acgtcgtcct ccccgaaccc ttcgcgctcg atgacctcct cgagcaggcg ggtctggtag
      481 gggatcctcg cgggcgtctg gaagccgccc tggatcacct cgtcgtcgag ggcgtggccc
      541 cgccaggtgc cgtcggcctg cccgtcccgc cggtcaatgt cggggaagta accggccagc
      601 tcggggaggt cgttcgcgta cccggggaag cggtagaact cgaggaggtg ctccggcagg
      661 ttccagcggg tgccctccgc ccccagcgtc tccgcgttcg gcgcctcgcg gaggaccacg
      721 agcggcttcg ggttgccctc ccaggccgaa ccgtgcccga gcatcccgag ctggatgccg
      781 accgtgccca gcaggccggc cttggcccgt cccccggtct cctgcatcca gaggtcggcg
      841 agcgagggtc ggagcaggag gtcggggccg gtgcgccacg gcgggacgat gcggtccccg
      901 atccggatgc tgtggccgac gaggccgtgg gtccgaggga acgtcccggt cccgatgatc
      961 ccgtggccct gggcggagct cgtgggagag gacccggcga acgcctcctc gtaccaggcc
     1021 ccctccggga tcaggcggcg gaggttcggc cagcgacccc gccactcgtc caggagggag
     1081 cggcccgcgc cgtcccagat cacggtgacg atcagcttgg gcggcccgcc gggatcctcg
     1141 accagggcct cggtgagggg cttgccgtcc ggcgtgggga agtcgagccc gagcatctgg
     1201 gcgtgcgtgg gggcgatgtc cgccagggtg gccgcgcgcc ggacgcggcc ctgtggctcg
     1261 acgtggccgg ggccgtacca gaacatcggc acgtgcgagg tgtacgtcca cggcccgacg
     1321 tgggggaggc ccgcccccac gaagttcggc tcccggggga ggatctgcag ctccgcgctc
     1381 cgctcggggt ggtagccccg ggcgatccgc aggatccacg gcgccgggag gccgcacgcc
     1441 acctcctcca tccgcgtcga ggggatgggg gcgacggagg gggatccgcc gctcggctcg
     1501 gggctgcgcc tcgccgccgg cgcccccgcg gagcacgcgg cggcgaccag cgacagcgcg
     1561 acgaggagcg ctcgtgccgg agacatacgg acgacaggga acaccatctt gagcagaacg
     1621 gcaacgatgc tactggtaag gtgcccccga tgaccgagcg agcgagggac gcccttcgtc
     1681 gagagctcta ctggggggac gagcccccgg ccgccggccc cacgcgggag ctccgcgccc
     1741 ggatcgagag gctcgccgat tcgcttcagg cggacctggc ccggccgttc ccggcggacc
     1801 cgtccttctc cggcggctcc cggtggcggc tgggggtgaa gcggttcctg taccgcctca
     1861 accgtcccct gacgcgacgc cacgaccgcg tcgcggccga gctggccgcg atctcgctcg
     1921 agctcctcga ccatctgggg cgggtcgagg ccgagctccg tcggatcgag gagcggctcc
     1981 ccgaggaggg ggaggccccg tgaggatcgc cgtctgccac ccgcagacgc cgttcacgag
     2041 gggcggcgcc gagatgcaca tggaggggct cgtggccgcc ctccgcgccg acggccacga
     2101 cgtcgacgtc gtgacgatgc ccttcaagtg gtacccgccg tccgagatcg tccaccagat
     2161 ggcctcgtgg cggagcgtgg acctgtccga ggcgaacggc cagcggatcg acatggtgat
     2221 tgccctgaag ttcccggcct acctcgtggc tcacgagcgg aaggtggtct ggctgatcca
     2281 ccagcaccgg acggcctacg agctgtggga tcatcccgag tacggggacc tccgcctcca
     2341 cgaggacggc gatgccgtcc gggacatgat ccaccgcgcc gaccgcgcgg gcctggcgga
     2401 ggcgaagcgg atctttacga actcggcgaa cgtccgggat cggctgcggc gctcggtggg
     2461 gctgtcggcc gacgtcctct accaccggtc gccgctgtgc gaggcgctgc tcgaggtcga
     2521 gccgggcccg cacggcgacc acgtgctgtt gcccagccgg ctcgagcccc tgaagcgcca
     2581 gtcgctggcg atcgacgcca tgcggcacgt gcggtcgggg gcgcggctcg tcctcgtagg
     2641 gaagggtccc ggcgaggacg cgctgcgccg gcgggtcgcg gaggcggggc tcgaggatcg
     2701 ggtcgagctc gccgggcccg tcccggacga ggagctgatc gagctctacc gcacggcgct
     2761 ggccgtcttc tacggcccct acgacgagga ctacggctac gtgacgatcg aggcgatggc
     2821 ggccagccgc cccctggtga cctgcacgga cagcggcggc ccgctcgagc tggtccacga
     2881 cggggaggac gggcgcgtcg tggcgcccga gcccaaggcc atcgcggggg cgatcgacgc
     2941 cctgcaccag ggccgcgacg cggccgcgcg catgggggcg gccggccgct ccctggtcga
     3001 ggcccgggtc cccccgtggc ccgaggtggc ccgccggctg ctgtcgtgaa ggtcgcgttc
     3061 ctctcgccgc tgccgcccgc gccgaccggg atcgcggcgt actcgcgagc ggtgctcgat
     3121 cggctcgagc gccgcgggat cggcctcgag ctgatgccgc gcgcgccgcg ctcgctcgag
     3181 gaggccgagc ggctggccga gacgagcgat ctcgccgtct accacgtggg gaacaacccg
     3241 ctcttccacc gccacatcta cggggcggcg atccgtcgtc cggggctcgt ggtgctccac
     3301 gacctcgcgc tggacgacct catccggggg ctcgacgccg cgtcggatcc cacggccgga
     3361 ccggctcggg ccgaggctcg cgcggcggcg cgggccctgc ggacgagcgg cctcgacgtc
     3421 gacccgccgc tcgccacggc ctggtccgcc cacctcgtcc gcagggcccg cggcgtgatc
     3481 gtccacgcgc ccttcggcgc gcggtacctg gaggccttcg gctgccgcac gcccgtccac
     3541 gtggtcccac atccgccggt gccggagccg tcggggccgc gcgctgcgcg agccgaccgc
     3601 cgcgttcgcc gccgcctggc cgggcggggt gccgtcgtgg gcgtcctcgg agacatcggc
     3661 gccgcgaagg ggatcgaggc ggtgctcgac gccgtggccc ggctcgggca gcccgcggtc
     3721 ctggcgatcg tgggccgacg gatccccggg ttcgacgtcg cgggggccgt gggccggagc
     3781 ggcctggggg atcgcgtcct cgtggaccag gacgtctcgg acgcagtctt cggcgcgtgg
     3841 ctgcgagcat gcgacgtcgt cgtcaacctc cgccatcccc accgaggaga ggtgagcggc
     3901 accctcgtcc gagcgatgca ggcgggcgtc ccgaccgtcg tgagcgccac cggcacgtac
     3961 ctcgacctcc ctccggatgc cgtggttcgg gtgccggccg gccctcccga ccccgaggcc
     4021 ctggtcgagg cgctcggcgt gctcctcgcc caccccgcgc gccgccgagc gatcggggag
     4081 cgggctcggg agcacgccga gcgcctcgac cgcgaggacg ccaccgccga gggctacgcg
     4141 cgcgcgatcg aggagaccct ccggatcgtc cgcgacccgc gccggccggc cgtggcccgg
     4201 tgggcggccg ccctcaggga cgtcggcgcg ggggacgagg tgatcgagcg cggccttggg
     4261 tccgagtacg tggacgcgct gacgtcgctg gaccccgacg ctacgacctg atgtcgcccg
     4321 gggatcggcc ctcgaccccg ggcggccact cgggcaggtc cccgatgtac tcgagaggct
     4381 ccccctggca gtggcccgcc gccggctggg gcgtcggcct ccgcggcggg cgatcgaagt
     4441 cgaagacgtg ctcgaagttg tgggtgtccg cgatcgcgtc ggtcagatag ggcaggtccc
     4501 agttgtcggc gatgaacttc agcggcgagg tgaactcgcc ctccgcgtcg tcgacgtacc
     4561 ccttcctcgc gtagggcgag atcacgaaca tcgggacgcg cacccccagc tcccgctcgc
     4621 cgatcaccgg gggcaccacg tggtcgtaga accctcccca ctcgtcccac gtgacgaaga
     4681 tcgcggtgtg ctcccacatg tcgctgcgca tgatgccgct gacgatgtcg ttcacccagt
     4741 tcatcgcgtg gcacgtgctg aacggcgggt ggtccgagag ctggaaccgg ggcacgaccc
     4801 aggtcaccgg gggaagagcg ttcgcctcga tgtccacaag gatctcgtcc accggccaga
     4861 tgtgctcgtc ccacagctcg gtgtggaaga cgtccgggat ggccgcgtac gcgttccaca
     4921 tgtacccgga ctggtgcggc tcgggcgagt agtacgccca gtcgatgtcc cgctcggtca
     4981 gctgctcacc catcgtgggg aggtggaacg tcgtgctgtg cttcgtcacg ttgccgtccg
     5041 ggtcctccac gagcacgaag acgtcgtcgc cgtaggcgtc gatgccccag gacttgatcc
     5101 acttgtcgcc gtccttgaag acccggatgt tctcggggtt gtcgatcgcg ccgccgttcg
     5161 tccccgccac gaagaagagg tggttcgggt acgagggccc gttcaccgag gcgaagaagt
     5221 tgtcgcacag cacgtagtcc ttcgcccagt tccagtagat cgggagattc tgggccgaga
     5281 actgcgtgta ggcgaagtac ggccccagct cgccgagcgc gaagttgtcc atctggccgt
     5341 cgttcacgca tttgatcgcg gccgagcggt cgtgggggat gtcgcccgcc agccactcgg
     5401 gggcgggggt gaggggccgc tcacgcccca ggtccacccc caccgtgacc ccgttcacgc
     5461 cggggaacgt gccgaacatg tgatcgaagc tgcggttctc gagcatgatg taaaccaccc
     5521 gcttgatcgg ccagcgcgtg tccacgtcgt ccaggttctg cccgaacgtg gggcggaccc
     5581 ccgacgtgca ggaggcgagg agggcccccg ccgccgagcc ggccgagagc cgcaggaagt
     5641 cgcggcgcga gaagcgtcgg tgcgcgatga agctgtccat cgaacggtcg tccaccccca
     5701 gggttgcgtg ccggagcctg tatcctagcc tgcgttcccg gacatgatgg gggtctcgcg
     5761 cgtgtcgaag cgatggatcg gggggctcgc ggcggtcctg atcctcgtcg cgtgcggagg
     5821 cgaccaggcg tccgataccc ggcaggagga gccctccctg ccgaccgagg tgagcacggc
     5881 ggaggagacc ccaccgcctc cgcgcgagcc cgacgggttc gagcccgagt cgggtgaggc
     5941 cggcgacacc tgcgtgaacg gctggaaccg ccccctccgc gacgatccca tcgtgctgga
     6001 ggggcttcgg atcatccgcc gacacatggg cgtggacggc ggcttctcgc tccaagattt
     6061 ccggtacttc gagggacccg agtccccgcc cagcgacaag gggtacctcc tgaccgtccg
     6121 gcgctggtac tacaagggtc acctgaagag cgatcccggg ttccgtggcc gctggctcgt
     6181 ggagtggcgg gagttcgggc cgggcatcgc ggcggtggcg ccctacgaca cggagggctt
     6241 ccgctcgccg gactgggtgg gcttcgagta ccgggagcgg ctggagcctc gctccttcga
     6301 ggggatcccg ggggagtggc ggggcgagcc cttcgacttc gtgcggggcg cgcgggagcc
     6361 cgacatccgg gtcgagggcc tccccgccga ggtccgcggc tgcctcgccg gcacctgatc
     6421 cccgcttgac ccctccagcc tccggggcgt accgtatcgg gtatccgaac gtgtgttcga
     6481 gtccctcggg gtgcgcatgg ggttcgtcca tctccactgc cgatccgcct tctcgctgaa
     6541 ggacggtgcg ttcctgcccg aggcgctggc cgcggcggcc gccgagctgg ggatgccggc
     6601 cgtcgccctc accgatcgcg acgccctcta cggcgccgct cggttcgtgg acgcctgtga
     6661 gcgggtcggc gtgcgcccga tcctcgggtc cctgctcacc ctcgacgagg gcgaggtcct
     6721 cctgctggcc cggaccgacg ccgggtacgc gaacctctgc cggctcatca cgcacgccca
     6781 catgtccggg gagcggggag agccctcggt gacggcgggc gaggtcctca ggcgggccga
     6841 cgggctcgtg gcgctgctgg gaccggggtc gccgccggga cggttggcct tgcgcggcag
     6901 gctcggggcg gcgcgcgagc tgctgcgccc gtggcgcgag gcgttcgggg gctggtgctt
     6961 cgtcgaggtg cggaacctcc tggagccgat gagcgcggcg gaggtccgca cgctgctgcg
     7021 gctggccgac gaggcggggg tcccggccgc cgccacgaac gccgtccgcc acctcgttcc
     7081 cgaggacgcc ttcgtggccg acgccctgga gtgcatgcgc gagctcgtcc ccgtggccga
     7141 gcaccacctc acccgctgga acaacgaggg gttcctcaag cccgcggccg cgatgcgtgg
     7201 gctgttcgcc ggccgcccc
//
LOCUS       DASUTW010000031         2823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_269119, whole genome shotgun sequence.
ACCESSION   DASUTW010000031 DASUTW010000000
VERSION     DASUTW010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269119"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1405
                     /gene="ffh"
                     /locus_tag="VF058_01150"
     CDS             53..1405
                     /gene="ffh"
                     /locus_tag="VF058_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX6206942.1"
                     /translation="MFEQLTDRLGATFKKLRSRGKLHPKQVDKALGDIRDALLDADVA
                     VEVADSLLDRVRERSMGEEVMKSVTPGQQVVKVVQDEMTATLGGEHEPIVLPDASPVV
                     IMMAGVQGSGKTTTCAKLAQHLKGKGRSVLLCAADLARPAAIEQLFQLGEEIGVPVVS
                     DGKDPVKVAKSALRQAKKESHDVLIVDTAGRLHVDEEMMREVGRVRATLKPHHSLMAA
                     DAMTGQDAVRQAQAFMEHADLTGFILTKVDGDARGGAALSITEVTGRPIFFAGMGESP
                     KDLEPFHPDRMASRILGMGDVVSLVEKAQREMDEDAARESAEKMLKAQFSLEDFLTQL
                     REVKKLGPLEDLLAMLPGVPGGAGLKDLQVDENELVRAEAIISSMTLEERQNPALLNG
                     SRRSRVAKGSGTTVSQVNSLLKQYDETKKMMKRLGGGGLGGLAKGLGGGGMKLPKGLG
                     "
     gene            complement(1409..1948)
                     /locus_tag="VF058_01155"
     CDS             complement(1409..1948)
                     /locus_tag="VF058_01155"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX6206943.1"
                     /translation="MPKRLTYADLLERFPEETNQRIELIDGELLMPPAPSVRHQDVVL
                     EIGVRLRLDAERRGGLVLASPMDVLLTEHDVVQPDVLYLLPEHAEGVERPLTRVDLAV
                     EVSSPSTRRTDTVRKRELYERHGVPEYWFVDLDADRILVYRLEEGRYGEPQVLEPDHE
                     LTTPLLPGFSALVGDLLTR"
     gene            2048..2533
                     /gene="rpsP"
                     /locus_tag="VF058_01160"
     CDS             2048..2533
                     /gene="rpsP"
                     /locus_tag="VF058_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007470773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX6206944.1"
                     /translation="MVKIRLMRMGAKKRPFFRVVVADSRSPRDGRFIENIGKYHPLEH
                     PSLIEIDEERAMHWLQNGAQPTQPVRVLLEKTGVWDKFQGGPGTPVPPPPDEEAAPPR
                     AEAEEAAAEEAVAEAPGGEAEAEAEAPAEAEAVEAEPEEAEEPQAEAEPQAEEGEEKP
                     D"
     gene            2533..2796
                     /locus_tag="VF058_01165"
     CDS             2533..2796
                     /locus_tag="VF058_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX6206945.1"
                     /translation="MLEVVEYVAKFLVDEPDKVEAEEFEEDDGTVVIELTAAPDDMGK
                     VIGKNGRVARALRQLVRAVGTREGVRATVDIVDELSEPEDDEE"
BASE COUNT          475 a          945 c         1037 g          366 t
ORIGIN      
        1 cctcgagctc gtggagcggc tcacctcctg acccccggct acactggggg cgatgttcga
       61 gcagctcacc gaccgcctcg gcgcgacctt caagaagctc cgctcccggg gcaagctcca
      121 tccgaagcag gtggacaagg ctctgggcga catccgcgac gctctcctcg acgccgacgt
      181 tgccgtggag gtcgcggact ccctgctgga ccgggtgcgg gagcgatcga tgggcgagga
      241 ggtcatgaag agcgtgaccc ccggccagca ggtcgtgaag gtcgtgcagg acgagatgac
      301 ggccacgctg gggggcgagc acgagcccat cgtcctcccc gatgcgagcc ccgtcgtcat
      361 catgatggcc ggcgtccagg gctcggggaa gacgacgacc tgcgccaagc tggcccagca
      421 cctcaagggc aagggccgga gcgtcctgct ctgtgccgcc gacctggccc gccccgccgc
      481 aatcgagcag ctcttccagc tgggggagga gatcggcgtg cccgtcgtct cggacggcaa
      541 ggacccggtg aaggtggcca agagcgccct gcgtcaggcg aagaaggagt cccacgacgt
      601 cctcatcgtt gacacggccg gccgcctcca cgtggacgag gagatgatgc gcgaggtggg
      661 ccgcgtccgc gccacgctca agccccacca ctcgctgatg gcggccgatg cgatgaccgg
      721 gcaggacgcc gtgcgccagg cccaggcctt catggagcac gccgacctca ccggcttcat
      781 cctcacgaag gtggacggcg acgcccgcgg cggcgcggct ctctccatca ccgaggtcac
      841 cggccgaccc atcttcttcg cggggatggg ggagagcccg aaggacctcg agcccttcca
      901 ccccgaccgg atggcctcgc ggatcctggg gatgggcgac gtggtctcgc tcgtggagaa
      961 ggcccagcgc gagatggatg aggacgccgc ccgcgagtcg gccgagaaga tgctgaaggc
     1021 ccagttctcg ctggaggact tcctcaccca gctccgcgag gtgaagaagc tcgggccgct
     1081 cgaggacctg ctggcgatgc tgccgggcgt ccccggcggc gcgggcctca aggatctcca
     1141 ggtggacgag aacgagctcg tgcgggccga ggcgatcatc tcctcgatga cgttggagga
     1201 gcgtcagaac cccgcgctgc tcaacgggag ccgccggtcc cgtgtggcga aggggagcgg
     1261 gaccaccgtc tcgcaggtga actcgctgct gaagcagtac gacgagacga agaagatgat
     1321 gaagcgcctc ggcggcggcg gtctcggcgg cttggccaag ggcctgggcg gcggggggat
     1381 gaagctcccc aagggcctcg gctgaggctc agcgcgtgag caggtctccg accagggcgg
     1441 agaacccggg cagcagcggc gtcgtcagct cgtggtcggg ctccaggacc tggggctccc
     1501 cgtaccggcc ctcctcgagg cggtagacga ggatccgatc cgcgtctagg tccacgaacc
     1561 agtactcggg cacgccgtgg cgctcgtaga gttcccgctt cctcaccgtg tcggtgcggc
     1621 gcgtgcttgg cgaggacacc tcgacggcga ggtccaccct cgtgagcggc cgctccaccc
     1681 cctcggcgtg ctccggcagg aggtacagca cgtcgggctg gaccacgtcg tgctcggtca
     1741 gcagcacgtc catcggggag gccagcacga gcccgccccg gcgctccgcg tcgaggcgga
     1801 gtcgcacgcc gatctctaag acgacgtcct ggtggcggac gctcggcgcc ggaggcatca
     1861 gcagctcgcc gtcgatgagc tcgatccgct ggttcgtctc ctccgggaac cgctccagga
     1921 ggtccgcgta cgtaagcctc ttcggcatcg gtcgtcgctc cacaggtcga aacgctatcc
     1981 tgagcctacg acatgaaccc gcgcgggggt gccctcgcgt ccgggcgacc gatcaggaga
     2041 cgtacgcatg gtgaagatca ggctgatgcg gatgggcgcg aagaagcggc cgttcttccg
     2101 cgtggtggtg gccgactcgc gcagcccgcg cgacggccgc ttcatcgaga acatcgggaa
     2161 gtaccacccg ctggagcatc cctcgctcat cgagatcgac gaggagcggg cgatgcactg
     2221 gttgcagaac ggcgcccagc ccacccagcc cgtccgggtc ctgctggaga agaccggcgt
     2281 ctgggacaag ttccagggcg gccccggcac cccggtcccg ccgcctcccg acgaggaggc
     2341 agcaccgcct cgcgcggagg ccgaggaggc cgcggccgag gaagcggtgg ccgaggctcc
     2401 gggaggggaa gccgaggcgg aggccgaagc gccggcggag gccgaagcgg tggaggccga
     2461 gcccgaggag gccgaggaac cccaggccga ggccgagccc caggccgagg aaggcgagga
     2521 gaagcccgac tgatgctcga ggtggtggag tacgtcgcca agttcctcgt ggacgagcca
     2581 gacaaggtcg aggccgagga gttcgaggag gacgacggca ccgtcgtgat cgagctaacg
     2641 gcggcccccg acgacatggg gaaggtcatc gggaagaacg gtcgcgtggc gcgggccctc
     2701 cgccagctcg tccgggcggt cgggacccgc gagggcgtcc gggcgaccgt cgacatcgtc
     2761 gacgagctct cggagcccga ggacgacgag gagtaggcca ggcggggcca tgaccccgga
     2821 gcg
//
LOCUS       DASUTW010000032         2057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1565256, whole genome shotgun sequence.
ACCESSION   DASUTW010000032 DASUTW010000000
VERSION     DASUTW010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565256"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VF058_01170"
     CDS             complement(<1..790)
                     /locus_tag="VF058_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004092572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX6206946.1"
                     /translation="MTELDVRLVDVVKSFGDVVAVDHVDLEVRRGDFFSLLGPSGCGK
                     TTTLRMLGGFEQPDHGRIELRGLDVAGVPPHKRDVNTVFQSYALFPHLDVFENVAYGL
                     RRKRTPREEVGRRVAQMLELVDLPGFEPRRPGQLSGGQAQRVALARALVNHPQVLLLD
                     EPLGALDLKLRKQMQLELKRIQHEVGITFIYVTHDQDEAMTMSDRIAVMRSGRIEQVG
                     APEEVYEQPATEFVAGFLGASNLLTGTVEARDGAHAVVVLTSGEK"
     gene            1062..1274
                     /locus_tag="VF058_01175"
     CDS             1062..1274
                     /locus_tag="VF058_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206947.1"
                     /translation="MADNPWSGFGEAWAVTTTLIAGMVAWGGIGYLLDLWLGFRWLFL
                     PIGMVVGMGGGIYLVYIRYGREDRET"
     gene            1249..1713
                     /locus_tag="VF058_01180"
     CDS             1249..1713
                     /locus_tag="VF058_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206948.1"
                     /translation="MEEKIARPEHAMVRKALAAGAAAAPVAFGAVFVAGGGGAALSAL
                     LGVVVVVLNFAAHGLSLAWAAGVSIPAVQAVALGGFVVRMGLIAGTMVAFDRAAFFSP
                     LVFGLTVIAATLALLVYEARLVRAGLGGALEIPPDPVAVAAREALRLKESSP"
     gene            1710..>2057
                     /locus_tag="VF058_01185"
     CDS             1710..>2057
                     /locus_tag="VF058_01185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX6206949.1"
                     /translation="MTGLLAQAHRPPEPFEAPGVEILDPPALWEFTLFGIHFDITRVT
                     LYLFLAVALVVILFVLAARRPQLVPRGVQNFVESIVQFIREEIALQVIGREGLTWVPF
                     LTALFVFILIGNIF"
BASE COUNT          265 a          729 c          713 g          350 t
ORIGIN      
        1 ccttctcgcc cgaggtcagg accacgaccg cgtgcgcgcc gtccctggcc tcgaccgtcc
       61 ccgtgagcag gttggacgcg ccgaggaacc cggccacgaa ctcggtggcc ggctgctcgt
      121 agacctcctc gggggcgccg acctgctcga tccgccccga ccgcatcacg gcgatgcggt
      181 ccgacatcgt catcgcctcg tcctggtcgt gcgtgacgta gatgaaggtg atcccgacct
      241 cgtgctggat ccgtttcagc tcgagctgca tctgcttccg cagcttcagg tcgagtgccc
      301 ccaggggctc gtccagcagg agcacctggg gatggttcac gagggcccgg gccagcgcca
      361 cccgttgggc ctggcccccg gagagctgcc ccgggcgccg cggctcgaac ccgggcaggt
      421 ccacgagctc gagcatctgg gccacgcggc gcccgacctc ctccctcggg gttcgcttcc
      481 ggcgcagccc gtaggccacg ttctcgaaga cgtcgaggtg ggggaagagg gcataggact
      541 ggaagacggt gttgacgtca cgcttgtgcg gcgggacccc ggccacgtcc aggccgcgca
      601 gctcgatccg tccgtgatcc ggctgctcga acccgcccag catccgcagc gtggtggtct
      661 tgccgcatcc cgacggaccc aggaggctga agaagtcccc ccgtcgcacc tcgaggtcca
      721 cgtggtccac cgcgacgacg tccccgaagg acttgaccac gtcgaccagc cggacgtcga
      781 gctccgtcac ccctcacccc cggacagcgt acggccgcgc ctcccgccgc gcccgtacct
      841 gtcgtgggga agggcggcct ccgtttgtac gaaccggcgc aagcggaagg gtaccgcagg
      901 ggccccgcgt aggcgcgcgg ggccgggccg gggaccgcgc gacttcgtga aagttttcac
      961 aaagttgggc gcaggacggt ccatatacgc tctgacctgc ggaaacgagt ccccccgagg
     1021 tttgacgctg ccgaagcgcg accgatatac tctctcggcc catggcggac aacccctgga
     1081 gcggattcgg ggaggcctgg gcggtcacca ccacgctgat cgcggggatg gtcgcctggg
     1141 gcgggatcgg ctacctgctc gacctgtggc tggggttccg gtggctgttc ctcccgatcg
     1201 ggatggtggt cggcatgggc ggagggatct acctcgtcta catccggtat ggaagagaag
     1261 atcgcgagac ctgagcacgc gatggtgcgg aaggcgctgg ccgcgggagc ggccgctgcg
     1321 cccgtggcct tcggcgccgt cttcgtggcg ggagggggcg gcgccgccct ctcggcgctg
     1381 ctcggggtcg tcgtggtcgt cctgaacttc gcggcccacg ggctgtccct ggcctgggct
     1441 gctggggtct cgatccccgc cgtgcaggcg gtggcgctcg gcgggttcgt cgtccggatg
     1501 gggctgatcg ccggaacgat ggtcgccttc gaccgagccg cgttcttctc gcctctcgtg
     1561 ttcggtctga cggtgatcgc ggcgacgctc gccctgctcg tctacgaggc ccggctcgtg
     1621 cgcgccgggc ttgggggtgc cctcgagatc ccgcccgacc cggtggctgt ggcggcccgc
     1681 gaggccctgc gcctcaagga gtcgagcccg tgaccgggct gctggcccag gctcaccgac
     1741 cccccgagcc gttcgaggcc ccgggcgtcg agatcctcga cccccccgcg ctctgggagt
     1801 tcaccctgtt cgggatccac ttcgacatca cccgcgtcac gctctacctg ttcctggcgg
     1861 tggccctcgt cgtgatcctc ttcgtgctgg ccgccaggcg gccccagctc gtgccccgag
     1921 gcgtgcagaa cttcgtggag tcgatcgtgc agttcatccg cgaggagatc gcgctgcagg
     1981 tgatcggtcg cgagggcctg acgtgggtcc cgttcctgac ggcgctcttc gtcttcatcc
     2041 tcatcggcaa catcttc
//
LOCUS       DASUTW010000033         7992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_269338, whole genome shotgun sequence.
ACCESSION   DASUTW010000033 DASUTW010000000
VERSION     DASUTW010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269338"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VF058_01190"
     CDS             complement(<1..522)
                     /locus_tag="VF058_01190"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX6206950.1"
                     /translation="MSGRRARARERRTAREQAKQRALQTDFTIITGLSGAGRSEAAKC
                     LEDLGYFVVDNLPPALIGKMAELVAQSEEPRRVAIVADARGGVFFADLAKGLEELREA
                     GTPARILYLEADDETLVMRFAATRRRHPLAPADRMVEGIRKERELMEALREEADLIID
                     TTGLTPNGLRERIR"
     gene            complement(500..2378)
                     /gene="uvrC"
                     /locus_tag="VF058_01195"
                     /pseudo
     CDS             complement(500..2378)
                     /gene="uvrC"
                     /locus_tag="VF058_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292444.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
     gene            complement(2399..3961)
                     /locus_tag="VF058_01200"
     CDS             complement(2399..3961)
                     /locus_tag="VF058_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206951.1"
                     /translation="MIRGRLPLLALVAVVAASCGPGDVRVSSVPIEEVGFVTQVVDVQ
                     GEAGAGISIAVDAEGSPHLAYLAFERELEEGEQPPPPDPNLPLFPAVRHAHQAQGVWS
                     RGTAAEELGDLTQDDETAIAVDGDGAEHVAWTEGGRLRYSSNAGGAFSEPEIVARADE
                     LHGVAIAVAEDGTPSISFYDVPGGPEGPAALVRVATPAGGGWDVETAAEADPNQPMST
                     GIGITQDGAIVAYGSGPTTYVARDLGDLWESEEVDGGGLGVALDLDGDGNPHVSYYTS
                     DGTVRHAHSIGGGPWETSDVGSAGQGPGSDWATSISVDPEGVHWVAWQTAEGLGLASN
                     EEGSFQEHELPGTQGGARPQVATGAEGAVFVAWHDTEDGTANVSSRTAEEPLLAGPPE
                     TGAPQPGPTGDGGGDGGGGQGGPPPCEPSGTELAIVAPVGAVADGFDTDCLAAPAGEA
                     FTIDFDNQDTGVPHNVSIYADDTAAERFFEGEIITGPEQITYEPDPIDDAGVFYFHCD
                     VHPNMNGAFVVQ"
     gene            complement(4008..6857)
                     /gene="uvrA"
                     /locus_tag="VF058_01205"
     CDS             complement(4008..6857)
                     /gene="uvrA"
                     /locus_tag="VF058_01205"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX6206952.1"
                     /translation="MGTEKLVIHGAREHNLKNIHLELPRNELIVFTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMEKPDVDFIEGLSPAISIDQKSTSKNPRSTVGT
                     ITEIYDYLRVLYARIGIPHCPRCGRPIGRQTTDQIVDQVAALPEGTRFQVLAPVVRGR
                     KGEYEKLLKDLAGPGFARARIDGEVRDLSEPIRLDRYYKHDIEVVVDRLVAKQGIRRR
                     LADSIETALELSEGLAAIAIRTDGTEEVVTFSQSFACTSCGLSFDEPAPRNFSFNSPY
                     GACPTCDGLGTRLEVDAELVVPDPDRTLEDGALAPWNSTRLEYWNRMLASVCEAHGIP
                     TGVPWRKLTKEQRKTLLYGSDDEVHIRYRNRYGRVRSYWTTYEGIIPNLERRHDETES
                     EFSRDRIEQYMREVPCRTCRGARLKAESLAVTVGELNISELTAKSVGRALTFLQELEK
                     DLSDRERMIAERLLREIRARLGFLVDVGLDYLTLDRAAATLAGGEAQRIRLATQIGSG
                     LVGVLYILDEPSIGLHQRDNQRLIDTLIRLRNLGNTLIVVEHDEATIRTADWIVDIGP
                     GAGAAGGEIVYSGEVKGLFDHPGSLTGEYLSGRREIPVPGVRRSASGWLEVKAAAEHN
                     LKGIDVRFPLGTFICVTGVSGSGKSTLVEDVLSRALQLKVQGSRVVPGKHRRLVGWER
                     VDKLVVVDQSPIGRTPRSNPATYTGVFDHIRRLYAQVPEARMRGYQPGRFSFNVRGGR
                     CEACSGEGQIKIEMHFLPDVYVTCEQCKGRRYNRETLEVRYKHRTIAEVLDASVDEAL
                     AFFQNQPTIARYLQTLSDVGLGYLRLGQPAPTLSGGEAQRVKLASELQRRSTGSTFYI
                     LDEPTTGLHFEDVRKLLGVVERLVAAGNTVVVIEHNLDVVKTADWLIDLGPEGGDAGG
                     EIVAEGTPEEVAAAPGSHTGSFLTEVLRHERAATG"
     gene            complement(6914..>7992)
                     /locus_tag="VF058_01210"
     CDS             complement(6914..>7992)
                     /locus_tag="VF058_01210"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6206953.1"
                     /translation="APLYDAISRAKGAWIASLLVQGAMAWRGPEEPLVEDAAVVLDGS
                     RIVYSGSARLAPEADEEVVGDWFLMPAAVDHHVHIGLADPRAVLAGGVTQVRDLGWPP
                     EEVFPLADISTGTDFDGPAIAACGPIVTAVGGYPTRSSWGPPGIGVEVSGPDEAAAAV
                     ARIAEQDPAAIKVALNTEAGPSLADAELVAVCEAAHERRLPVVAHVQGPGTTERALGA
                     GVDELAHTPWTERLSEAVVRGLVRRGVRIVSTLDIHSYGKVTPDLRSAVENLFRFHSA
                     GGTVLYGTDLGNGPIPPGIDVREAQHLAAARMGVEEILTAMTAWSLHPGARGDLVALD
                     GNPFEDLEALGRVRLVVRGGRVRA"
BASE COUNT         1061 a         2974 c         2783 g         1174 t
ORIGIN      
        1 ccggatccgc tcccggagcc cgttgggcgt gagccccgtc gtgtcgatga tgagatcggc
       61 ctcctcgcgg agcgcctcca tcagctcccg ctccttccgg atcccctcca ccatccggtc
      121 ggcgggggcc agcgggtgac gcctgcgggt tgcggcgaag cgcatcacca gcgtctcgtc
      181 gtccgcctcc aggtagagga tgcgggccgg cgtgccggct tcgcggagct cctcgaggcc
      241 cttcgccagg tcggcgaaga acacgccgcc cctggcgtcg gccacgatcg ccacgcggcg
      301 gggctcctcg gactgggcca cgagctcggc catcttgccg atcagggccg gcggcaggtt
      361 gtccaccacg aagtagccca ggtcctcgag gcacttcgcg gcctccgagc gtccggcgcc
      421 cgacagcccg gtgatgatcg tgaagtcggt ctggagcgct cgctgcttcg cctgctcgcg
      481 cgcggtgcgg cgctccctgg cgcgcgctcg gcggccgctc acgccgacct cctggtgagg
      541 gcttcgtgga cctgctgcgc gagcgccggg cccacgccgg ggacctccgc gatgtcgtcg
      601 ggcgaggcct tcgccaggcg cttcagcgag ccgaaccggc gcaggagggc cttcttccgc
      661 gcgggcccga tcccgggcac ctcgtcgagg ggcgaggcga gggcccggcg ctcgcgccgc
      721 ctccggtgga ggtcgagggc gaaccggtgc gcctcgtccc ggacgtgctg gagcaggtac
      781 agggcctccg agccccgcgc gatccggagc gggtcgggct tccccggcag gtacacctcc
      841 tccaggcgct tggccaggcc cgccgcgggg atctccaggc ccagctcgtt caggacccgc
      901 accgccgcgc cgagctggcc tcgccccccg tccagcacca cgagcgaggg ggggtaggcg
      961 aaccgcttcc gctccgaggc cggccgctcg cgctcctcca ggtaccgggc gaagcgccgc
     1021 cgcagcgtct cctccatgct cgccacgtcg tcctggccgg gcaccccctt gatccggaag
     1081 cgccggtagt cggagcgctt gggcaggccg tcctcgaaca ccaccatcga gcccaccttc
     1141 tccgtggccc cgaggttcga gatgtcgtac gcctcgatcc ggagcggcgc gtcctcgagc
     1201 cctagtgccc gccccagctc gtccagcgcc ctcgacctcg aggcgaagtc ggtggcccgc
     1261 tggagcttgt gccggacgaa cgcctccttc gcgttcgcgg tcaccacctg ctggagccgc
     1321 cgcttctccc cgcgctgcgg caccgtgatc tccacccggc cgccgcgccg ctcccccaac
     1381 caccgctcca gcacctcgcg gtcgagcggc caggcgggga cgaggacccg gggcgggacc
     1441 tcgtcccgct ccatgtacag ctcgcggaca agggaggaga cgagggccgg cgtgtcgagg
     1501 tcctcgacgc gatcggccac ccagccccgg cgacccatca cccgcccccg gcgcacgaag
     1561 aacacctgga acgccgcctc gaggtcgtcc tcgaccatcc cgatcacgtc caggtcctgg
     1621 ggcctgccca gcaccatctc ctgctgctcc atcgccctcc gcgcggcggc gatccggtcg
     1681 cggaacctgg cagcggcctc gaactcctgg cgctcggccg cgctccgcat ctcctcgtcg
     1741 agccgatcga ggacgggccg gtggtccccg tccaggaagt ccatcagggc ctcgacctcg
     1801 gcccggtagc cctcctcggt gacgccggtc cgctcgggga cgcagggccc gaggcaccgg
     1861 ccgatgtcgt agtagatgca cggccgtcga gcgcgccgcc gctgatcgaa gaacccgttg
     1921 gagcaggtgc ggacggggaa gacgcgggtc agcgagtcga gcgtgtcgcg gatcgcgtag
     1981 gcgtgcccgt agggaccgaa gcgccgcacg cccttccggc gcttgccccg catgacgtac
     2041 gcgcggggcc acgtctcccc caccgtgacc gcgaggtacg ggtacgactt gtcgtcccgg
     2101 tagcgaacgt tgaagcgcgg ccggtgccgt tggatcaggt tgtactcgag catgagggcg
     2161 tcgacctctc cggaggcgac gatccactcc acggctgcgg cggcgtcgag catcgcctgg
     2221 gtgcgggggt gctgaggctt cgcccagtag gtcacgagcc gcttccgcag ggacttggcc
     2281 ttccccgcat agatcacgcg cccgtccacg tcccggaaca ggtaggcacc ggggtcgtcg
     2341 gggatcgtcg aggtctcggg gcggcggagc gtggtcacgc gcccattcta gggcgggcct
     2401 actgcaccac gaacgcgccg ttcatgttgg ggtgcacgtc gcagtggaag tagaacaccc
     2461 ccgcgtcgtc gatcgggtcg ggctcgtagg tgatctgctc ggggcccgtg atgatctcgc
     2521 cctcgaagaa ccgctcggcg gccgtgtcgt ccgcgtagat cgagacgttg tggggcacgc
     2581 ccgtgtcctg gttgtcgaag tcgatcgtga acgcctcgcc ggcgggggcg gccaggcagt
     2641 cggtgtcgaa cccgtcggcc accgcgccca ccggcgccac gatcgccagc tcggtgccgc
     2701 tcggctcgca ggggggcggg ccgccctggc ctccgccgcc gtccccgccg ccgtcgccgg
     2761 tgggccccgg ctggggagcg ccggtctcgg gcggcccggc gagcagcggc tcctccgcgg
     2821 tgcgcgagga gacgttggcc gtcccgtcct cggtgtcgtg ccaggccacg aagaccgctc
     2881 cctccgcccc cgtcgccacc tgtggtcgcg ctccgccctg cgtccccggg agctcgtgct
     2941 cctggaacga gccctcctcg ttcgaggcca gccccagccc ctcggcggtc tgccacgcca
     3001 cccagtggac gccctcgggg tccacggaga tcgaggtggc ccagtccgag cccgggccct
     3061 gcccggccga gcccacgtcc gaggtctccc acgggccccc gccgatcgag tgcgcgtggc
     3121 gcacggtgcc gtcgctcgtg tagtagctga cgtgtgggtt cccgtcgccg tcgaggtcga
     3181 gggccacgcc cagccccccg ccgtcgacct cctcggactc ccacagatcc ccgaggtcgc
     3241 gggcgacgta cgttgtgggc cccgagccgt acgccacgat cgcgccgtcc tgcgtgatcc
     3301 cgatgccggt ggacatcggc tggttgggat cggcctcggc cgcggtctcc acgtcccagc
     3361 ctcccccggc cggggtggcc acgcgcacga gcgcggcggg accctcgggc ccccccggca
     3421 catcgtagaa cgagatcgag ggcgtcccgt cctcggccac cgcgatcgcg accccgtgca
     3481 gctcatcggc ccgggccacg atctcgggct cggagaaggc gccgccggcg ttgctcgagt
     3541 accggagccg ccccccttcc gtccacgcga cgtgctccgc gccgtcaccg tccaccgcga
     3601 tcgccgtctc gtcgtcctgc gtcaggtcgc cgagctcctc cgccgcggtg cccctgctcc
     3661 acacgccctg agcctgatgc gcgtgccgga ccgccgggaa gagcgggagg ttggggtcgg
     3721 ggggcggggg ctgctccccc tcctccagct cgcgctcgaa cgccaggtac gcgaggtggg
     3781 gactgccctc ggcgtccacg gcgatcgaga tgccggcgcc ggcctcgccc tgcacgtcca
     3841 cgacctgcgt gacgaagccc acctcctcga tggggacgct cgagacgcga acgtcgccgg
     3901 gtccgcagga agcggccacc acggcgacga gagcgagcag cggaagacgg cctcggatca
     3961 tcgcgagcga ttgtgtatca cgaacgcgac cgcggtgtca gaggggatca tccggtcgcg
     4021 gcgcgctcgt gccggaggac ctcggtaagg aaggagccgg tgtgcgatcc gggcgccgcc
     4081 gcgacctcct cgggggtgcc ctcggccacg atctcgcccc ccgcgtcgcc tccctcgggg
     4141 cccaggtcga tcagccagtc ggccgtcttc accacgtcca ggttgtgctc gatcacaacg
     4201 accgtgttgc ccgccgccac gagtcgctcg accacgccca gcagcttccg cacgtcctcg
     4261 aagtgcaggc ccgtcgtcgg ctcgtcgagg atgtagaagg tgctgccggt cgacctccgc
     4321 tggagctcgg aggccagctt cacccgctgg gcctcaccgc ccgagagcgt gggggcgggc
     4381 tgccccagcc gcaggtagcc cagccccacg tcggagagcg tctgcaggta gcgtgcgatc
     4441 gtcggctggt tctggaagaa ggccagcgcc tcgtccacgc tcgcatcgag cacctcggcg
     4501 atcgtccgat gcttgtaccg gacctccagg gtctcccggt tgtaccggcg ccccttgcac
     4561 tgctcgcacg tcacgtagac gtccggcagg aagtgcatct cgatcttgat ctggccctcg
     4621 cccgagcagg cctcgcatcg tcctccccgc acgttgaacg agaaccggcc gggctggtac
     4681 ccgcgcatcc gcgcctcggg cacctgagcg tagagccgcc ggatgtggtc gaagaccccg
     4741 gtgtaggtgg cggggttgga ccgcggcgtg cgcccgatcg gcgactggtc cacgacgacg
     4801 agcttgtcga cccgctccca gcccacgagc cggcggtgct tgccggggac gacccgcgat
     4861 ccctgcacct tcagctggag cgcccgggag aggacgtcct ccacgagggt ggacttcccg
     4921 gatccggaga cgccggtgac gcagatgaac gtccccaggg ggaagcgcac gtcgatcccc
     4981 ttcaggttgt gctccgcggc ggccttgacc tcgagccagc ccgaggcgga ccgccggacg
     5041 ccgggcacgg ggatctcgcg ccggcccgag aggtactcgc cggtcagcga gcccgggtgg
     5101 tcgaacaggc ccttcacctc gcccgagtag acgatctcgc cgccggccgc cccggccccc
     5161 gggccgatgt ccacgatcca gtcggcggtc cggatcgtgg cttcgtcgtg ctccacgacg
     5221 atgagcgtgt tgcccaggtt ccggagccgg atcagcgtgt cgatcagccg ctggttgtcc
     5281 cgctggtgca ggccgatcga gggctcgtcc aggatgtaga gcacccccac gaggcccgag
     5341 ccgatctgcg tggcgaggcg gatccgctgc gcctctcccc cggccagcgt ggccgcggct
     5401 cggtccagcg tcaggtagtc gagcccgacg tccaccagga agcccaggcg ggcccggatc
     5461 tcgcggagca ggcgctcggc gatcatccgc tcgcggtcgg agaggtcctt ctccagctcc
     5521 tgcaggaacg tcagggccct ccccaccgac ttcgccgtca gctccgagat gttgagctcc
     5581 cccaccgtga cggcgagcga ctcggccttg agccgggccc ccctgcacgt cctgcagggc
     5641 acctcacgca tgtactgctc gatgcggtcg cggctgaact ccgactcggt ctcgtcgtgg
     5701 cgccgctcga ggttcgggat gatgccctcg taggtcgtcc agtaggagcg gacgcgcccg
     5761 taccggttgc ggtagcggat gtgcacctcg tcgtcggagc cgtacagcag cgtcttccgc
     5821 tgctccttcg tcagcttccg ccacggcacg ccggtgggga tgccgtgggc ctcgcagacc
     5881 gaggccagca tccggttcca gtactcgagg cgcgtggagt tccagggggc cagcgctccg
     5941 tcctcgagcg tccggtcggg gtcgggcacg acgagctcgg catcgacctc gagccgcgtg
     6001 ccgagcccgt cgcaggtcgg acacgccccg tagggcgagt tgaacgagaa gttccgcggc
     6061 gcgggctcgt cgaacgacag cccgcagctg gtgcaggcga acgactgcga gaaggtcacg
     6121 acctcctccg tcccgtcggt gcggatcgcg atcgcggcca ggccctcgga gagctcgagg
     6181 gccgtctcga tcgagtccgc cagccgtcgg cggatcccct gcttcgccac gagccggtcc
     6241 accacgacct cgatgtcgtg cttgtagtag cggtccagcc ggatcggctc ggagaggtcc
     6301 cggacctcgc cgtcgatgcg cgcccgggcg aaccccgggc cggccaggtc cttcaacagc
     6361 ttctcgtact cgcccttgcg tccccggacc accggggcca gcacctggaa gcgcgttccc
     6421 tcgggcagcg cggcgacctg gtccacgatc tgatcggtgg tctggcgacc gatcgggcgg
     6481 ccgcagcgag ggcagtgcgg gatcccgatg cgcgcgtaca gcacgcggag gtagtcgtag
     6541 atctcggtga tcgtgcccac ggtggagcgg gggttcttcg aggtcgactt ctgatcgatc
     6601 gagatcgccg gcgagagccc ctcgatgaag tcgacgtcgg gcttctccat ctgccccagg
     6661 aactgacggg cataggcgga cagggactcc acgtatcgac gctggccctc cgcgtagatc
     6721 gtgtcgaagg ccagcgacga cttcccggag cccgacaggc ccgtgaacac gatgagctcg
     6781 ttcctgggga gctcgagatg gatgttcttc aggttgtgct cacgggcacc gtggatgacc
     6841 agcttctcgg tccccatgcg tgccatccta tcagcgtcga ggcgctcgaa catccgttcg
     6901 acgcgcggga tgctcaggcg cggacgcgtc cccctcggac gacgaggcgg acccgaccca
     6961 gggcctcgag gtcctcgaag gggttcccat cgagcgcgac gaggtccccg cgggcccccg
     7021 ggtggagcga ccaggcggtc atcgccgtga ggatctcctc gacgcccatc cgggcggccg
     7081 ccaggtgctg agcctcgcgg acgtcgatcc ccggcgggat ggggccgttg ccgagatccg
     7141 tcccgtacag gacggtccct ccggcgctgt ggaagcggaa gaggttctcc acggcggagc
     7201 ggaggtcggg ggtcaccttc ccgtaggagt ggatgtcgag cgtcgacacg atacggacgc
     7261 cgcgtcgcac caggcccctc acgaccgcct cggacagccg ctcggtccac ggcgtgtggg
     7321 ccagctcgtc caccccggcg cccagcgccc gctcggtggt cccggggccc tgcacgtggg
     7381 ccacgacggg gagccggcgc tcgtgggccg cctcgcagac ggcgacgagc tcggcgtcgg
     7441 cgagggaggg gcccgcctcg gtgttgagcg ccaccttgat cgccgcggga tcctgctcgg
     7501 cgatccgggc cacggccgcg gcggcctcgt cgggcccgct cacctcgacg ccgatccccg
     7561 gcgggcccca cgacgaacgc gttgggtagc cgccgacggc cgtcacgatg gggccgcacg
     7621 ccgcgatggc ggggccgtcg aagtcggtgc cggtggagat gtcggccagc gggaagacct
     7681 cctcgggtgg ccagcccaga tcccgcacct gggtcactcc tccggccagc acggctcgcg
     7741 ggtccgccag cccgatgtgc acgtggtgat cgaccgccgc gggcatcagg aaccagtcgc
     7801 ccaccacctc ttcgtcggcc tcgggagcga gtcgagccga gcccgagtag acgatccggg
     7861 agccgtcgag gaccacggcg gcgtcctcga ccaggggctc ctccggcccc cgccaggcca
     7921 tcgctccttg gaccagcagg ctcgcgatcc acgccccctt cgctcggctg atcgcatcat
     7981 agagaggggc gg
//
LOCUS       DASUTW010000034         2992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_398900, whole genome shotgun sequence.
ACCESSION   DASUTW010000034 DASUTW010000000
VERSION     DASUTW010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398900"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="VF058_01215"
     CDS             complement(<1..1327)
                     /locus_tag="VF058_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX6206954.1"
                     /translation="MKAVAYLRVSTKEQAEEGYSIAAQQQACERFATERGWDLVDVYS
                     DRGESARTSDRPQFQLMLRRITEDDAVRFLIVHKLDRLARNIRDYAEIREALERAGVE
                     LVSVTEGLEATASGRMVEGMLAVVAEWYSNNLSAEIRKGQAQKLREGGWPTAAPIGYR
                     NVREEPGAGQRRGRAVIVPDEQAPLVREAFELYAAGGWSIPALSEEMGRRGLRNRRGG
                     KVSRSRLHSILRSPAYVGKVPWKGAVYDGTHEGIVPQETFKRVQELLDAHGRARERQR
                     RHTHFLKGLLRCGACEGRLIYNVVKGRNKRDFPYFACASHFNGQKKCGEGYVPAGVLE
                     QAVEDLYRGIKLPPRFQERLEGILEDEVARSERHRAQVTRFASRRLDRLSGERERLLD
                     LYLGGDIDRETFRARKERIEAETRELEARMVGQTTQLAQARELVTLALRL"
     gene            complement(1485..2399)
                     /locus_tag="VF058_01220"
     CDS             complement(1485..2399)
                     /locus_tag="VF058_01220"
                     /inference="COORDINATES: protein motif:HMM:NF025186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HEX6206955.1"
                     /translation="MDARDVAILRALLEHKILTTSQLSILFFRSLRRCQQRLKDLTDL
                     GLLGSFDPRRGFAAGRLPDHQFLTELGASVLAHRDGVPRGHLPWVPDESYADNRNLRH
                     RMGVNAFFCALVEASRATDGHCLHRWAPERKVRTRAGEIQPDGFGRYLHPAGACQFYL
                     EYDRGTEGPAALTRKLRSYLRFAGGWGEKASFPNLLVVVPISSREENVEEGLVAAGRG
                     SGRKADVPLFIASERLLASLGVLGAVWRPPGEGDERLRLTELPAVDASLFDRTRCLGR
                     AWTDPGARSRISPLSAAPRFPTGHPREP"
BASE COUNT          383 a         1097 c         1027 g          485 t
ORIGIN      
        1 cgagcctcag ggcgagcgtg acgagctcgc gagcctgggc cagctgggtg gtctggccga
       61 ccatccgggc ctccagctcc cgggtctcgg cctcgatcct ctccttccgg gcccggaagg
      121 tctcccggtc gatgtcgcct ccgaggtaca ggtcgagcag gcgctcccgc tcgcccgaga
      181 gccgatccag acgccttgag gcgaagcgag tgacctgggc ccggtggcgc tccgaccggg
      241 ccacctcgtc ctccaggatc ccctccagcc gctcctggaa gcggggaggg agcttgatcc
      301 cccggtagag gtcctccacg gcttgctcca ggacgccggc ggggacgtat ccctccccgc
      361 acttcttctg tccgttgaag tgggaggcgc aggcgaagta ggggaagtcc cgcttgttgc
      421 gccccttcac cacgttgtag atcagccgtc cctcgcaggc cccgcaccgg aggagcccct
      481 tgaggaagtg ggtgtggcgc ctctggcgct ccctggccct cccgtgggca tcgaggagct
      541 cctggaccct cttaaaggtc tcctggggga cgatgccctc gtgggtaccg tcgtagactg
      601 ctcccttcca cgggaccttc cccacgtagg cgggacttcg gaggatggag tgcagccgcg
      661 agcgggacac cttccccccg cggcggttcc gaaggccccg gcgacccatc tcctcggaca
      721 gcgcggggat agaccatccg ccggcggcgt agagttcgaa ggcctcccgt accagcgggg
      781 cctgctcgtc ggggacgatc accgcccgtc cccggcgctg cccggccccc ggctcctccc
      841 ggacgttgcg gtacccgatg ggggcggcgg tgggccatcc cccctccctg agcttctggg
      901 cctgcccctt tcggatctca gccgagaggt tgttcgagta ccactcggcc accaccgcca
      961 gcattccctc caccatcctc cccgaggccg tggcctccag gccctccgtg accgagacga
     1021 gctccacccc ggcccgctcc agggcctccc ggatctcggc gtagtcccgg atgttgcggg
     1081 ccaggcgatc gagcttgtgc acgatgagga accgcaccgc atcgtcctcc gtgatccgcc
     1141 gcagcatcag ctggaactgg gggcggtccg aggtacgggc cgactccccg cggtcggagt
     1201 agacatcgac tagatcccag ccgcgctctg tcgcgaaccg ttcgcaagcc tgttgctgtg
     1261 cggcgatcga gtagccctcc tcggcctgct ccttggtgga gacccgcagg tacgcgaccg
     1321 ccttcacctg tccgcctcct tccgaagcag ctcctccgcg agggccagga gcgccctggc
     1381 caggggcctg aggctagcat cgagctgtga cccactcgcg tgcggcctct cgggcggcgc
     1441 tggcggccga ggcgcccgca ggcgatcggc ggcggtcctt cgcttcatgg ctctcgggga
     1501 tgcccggtgg ggaagcgggg cgcggccgac aggggggata tgcgcgagcg ggcgccgggg
     1561 tccgtccagg cccgccccag gcaccgggtc cggtcgaaga ggctcgcatc gacggcgggg
     1621 agctcggtca ggcggaggcg ctcgtcgcct tctccgggag gccgccagac tgcgcccagc
     1681 acgccgaggg aggccaggag acgctcgctg gcgatgaaga gcggcacgtc cgccttccgt
     1741 cctgatcctc ggccggccgc caccagcccc tcctccacgt tctcctcccg ggaggatatt
     1801 gggaccacca cgagtaggtt ggggaaggaa gccttttcgc cccatccccc cgcaaacctc
     1861 agatagctcc ggagcttccg cgtgagggcc gcggggccct ccgtcccccg gtcgtactcc
     1921 aggtagaact ggcaggcccc tgccggatgg aggtagcggc caaacccgtc gggctggatc
     1981 tccccggccc tggtgcggac cttccgctcc ggcgcccacc gatgcaggca gtggccgtcc
     2041 gtggcgcgcg aggcctcgac cagggcgcag aagaacgcgt tcacccccat ccggtggcgg
     2101 aggttccggt tgtccgcgta gctctcgtcg ggaacccagg ggaggtgtcc tcgcgggacg
     2161 ccgtctctgt gtgcgaggac ggaggctccg agctcggtca ggaactggtg atcggggagg
     2221 cgtccggcgg cgaagccgcg cctgggatcg aacgacccga ggaggccgag atcggtcagg
     2281 tccttcagcc gctgctggca tctcctgagc gagcggaaga acaggatcga gagctggctg
     2341 gtggtgagga tcttgtgctc caggagcgcg cggaggatcg cgacgtctcg cgcgtccatc
     2401 tgtgatgaga tctctcgaag ctcggttcga aggtcggtct tcctctcggt tgcacacata
     2461 ggctgggcac actccacccc ctttcgggcc gaacggcgcc cggatcggcc tcgtgacctg
     2521 cggcttcgcg ggaccgccgc ctcgtgctcg cgccgcccat gcgccgcctg ccgagccgac
     2581 gagtccctcg gccgcgtccg gggccctgcc cgggcgcgct cagtcggggt gatgggtcac
     2641 ctcgcgccca tcaccccctt gcgcctcgat cccgagcggc gaccgctcgc tccttcccgc
     2701 ccctgcgggc gcccccgtcg tgggttcccc ctgggtgggc cggggtcgga gccttcgggc
     2761 accgcgttgc gtccgggttc gccccgcgca tgccgatacg gcctcctgtc gtctccgagc
     2821 cggcctcttc tgaggtgctc gggcgcagga ccgtcttggg cccgacgcag ggtcccggcc
     2881 tctcgcggag caggttggtg tccgtccggt ccgcgccgga gttgcctcga cgggcaccgg
     2941 tcttgcggca ctgggcgagc ctccagcccc ctccaggtcg atcgccgtcc gt
//
LOCUS       DASUTW010000035         9114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1047006, whole genome shotgun sequence.
ACCESSION   DASUTW010000035 DASUTW010000000
VERSION     DASUTW010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9114
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047006"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..350
                     /locus_tag="VF058_01225"
     CDS             225..350
                     /locus_tag="VF058_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206956.1"
                     /translation="MHPVFVRRQVEIREWLERNKDTAAELRLSILLPLTRGTQPP"
     gene            384..1445
                     /locus_tag="VF058_01230"
     CDS             384..1445
                     /locus_tag="VF058_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012690186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6206957.1"
                     /translation="MWAIAVKEFRQLRRDRRTVGLMVMAPLLLLIVFGFAARFDVEEV
                     STIVVGEGSEAARGRLPELFEVVETMPGGRAEAVEGLREGRAAAAVVVGPEPEILLDG
                     AELFSAQAALRSLAGAGGPPGGPRPRVEVLFNPGLETSAVMIPAIAGMILVFVGTLIT
                     SLGVVRERQAGTLEQLAVMPFRPWEVFLGKVSPYFVVAVFDLVLVLGVGVLLFDVPFE
                     GSIGTFLIGSLLFLFVTLGMGILVSSVSQNQGQAIQLAMMLLLPQVLLSGMIFPIESM
                     AVGVRWIAYILPLTYFIEIARGVMLRAAPLDALWPYLGLLAALGLVVFGLAVLRFRRD
                     LGPARAGARRPAPAPEAAR"
     gene            1442..2248
                     /locus_tag="VF058_01235"
     CDS             1442..2248
                     /locus_tag="VF058_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012834465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6206958.1"
                     /translation="MSWGVWEISARYGDRPALEGVTLEPRPGSIAAVVGADGAGKTTL
                     LRVLAGARDADAGEVRRPAPERLGFVSQGSGVYEDLTVAENLAFAARAYGVEGHDDRA
                     AELLATTGLAEARERLGSQLSGGMRQKLGVAMAMVHRPDLLVLDEPTTGLDPVSRVEL
                     WTMVAAAAAEGAAVVVSTAYLEEAERATHVLVLDEGRPLVSGSPEEVLSSVPGVVLEV
                     PTRPEDVPSWRRGRTWRAWIREGDPPRGALRVAPDIDDAVMVAALSRARP"
     gene            2245..3102
                     /locus_tag="VF058_01240"
     CDS             2245..3102
                     /locus_tag="VF058_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012834464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6206959.1"
                     /translation="MTTLALARGVTRRFGEVVAVSDARLDLGRGEVVGLVGANGAGKT
                     TLIRVLLGLLPPTSGEVRLFGEAPSRRTRRRVGYVPQGLGLYPDLTVDENLRFAAAAY
                     GAPAPVTHDEVAGVGDWLVGELPLGLQRRVAFAAAVGHEPDLLVLDEPTSGVGPLGRA
                     RLWDDIHGAADRGVGVLVTTHYMSEAEQCDRLVVLAGGRVVAEGTLKEILGGLTVVRV
                     RTERWEDAFGRLRDAGLRLGLVGRSLRVVEARVEEVAGSLAGAGIEAEVAEAPATFEE
                     AFLMLSEGA"
     gene            3099..3686
                     /locus_tag="VF058_01245"
     CDS             3099..3686
                     /locus_tag="VF058_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013735051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX6206960.1"
                     /translation="MMVRTGRRPGESGTREAIVEAARGAFAEHGYEGATIRGIAARAG
                     VDPALVYHFHGSKEELFAAAMRLPFQPSEMIPSLLEGDPDGLGERILRLFLVVSDQPG
                     GSPFVALMRSASTNERAARMLREFLTREVIGRVARALGAPDAELRATLVGSHLAGLAM
                     ARYILRMEPLASADRDTVVAAVAPTLQRYLTGDLA"
     gene            3782..4897
                     /locus_tag="VF058_01250"
     CDS             3782..4897
                     /locus_tag="VF058_01250"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX6206961.1"
                     /translation="MKTRVGWLCVLAIVLVLLPSLAHAISPDLGAVLGVEEVVGVPSP
                     TDLRGSHPTEAMGSGGGALLSPPRVVPRSEWGADEGLRFRGRRAKPAFLPVRKLIVHH
                     TVTRRWEPPRRAIRRIYRSHTLSRGFADIAYHYLIAPDGRVFKGRYSGPPGTRRRDRP
                     WADRDRRFAVRGAHALAANDGTVGVALIGNYENAPLSLKARGSLVRLLAWLSDRYGID
                     PLGSSRYPTWGGRMVRTRDIAGHREYGRTACPGEEVRRELPAIRREVVGRLRQAARGS
                     PDVHGPLIYRVRVPKVGRRGAILRWTTNEPSRTAASVRAQGSSRGRTTVRPGLTRHHT
                     VRLRPLDRGTTYVFRLGGADAGGNVRWSAPHSLRTKP"
     gene            5217..8252
                     /locus_tag="VF058_01255"
     CDS             5217..8252
                     /locus_tag="VF058_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEX6206962.1"
                     /translation="MAVVLVVPTQASAVVLIDGLGGGEPEPLDARTAVLQPTADQLAA
                     AEALGATVRWNRFGTPQSLIRYGGPLASGVRGADAETAARGWLATNAGLFGLDGTDAL
                     ELDAAVPLRGTDAQVLVFRQRIDGVLVSPDGILTLGVKGSARSGFDVVHASSSLVGRD
                     ALEGERELTAREALVEAASDVGADLETGDLRRAGTSAGWRVFEAEALEGGSQLVRSVA
                     FPMPRRGVVPAYETVVSPEVDEGFRTFVHAETGEVLFRESVVHHAADDPEWETFPSYP
                     RTTPLNRPPFNYPSADTRQLWCWEGLPGCRVQAEDRSPHPGTPWDVDPVTGTPTFTTT
                     GNYAHSQENWFDFLFPGPTGYQPTSSTRTYRFPWTNQWFEERCNPDVLVVGSGNDIDA
                     ATTALFVGHNRMHNWAYHLGFTEHAWNAQLHNFGVGTAEGDPLIGMVQAGAVSHGPPV
                     YAGRDNAFMRTLPDGTPSQSAMFLWQPIPGAFYAPCVDGDYDMAVIGHEYTHMIENRM
                     IGKGNRRMGFHAGAMGESVSDFIAMEHMNEYGDLPGGDLAWAVGPYVTGNPVRAIRNY
                     NMSFPSAGAFPEEGRYPRVNPLNFSAVGYDIVGPQVHADGEIWSATNFDIRELFVARY
                     GGGGAQLQEQCANGERPASQCPGNRRWIQLAFDAFLLMPVAPTFLDARDAYLAADMLR
                     FGGANQDLLWQAFARRGFGEHASVTGTQDTEPVPSWESPLHEEATLVFDAVAKDEGGA
                     PIEADVFVGHYEARVTPAARTAAGMNELRILPDTPGGRHRAYEFTAHAEGYGHVRFRV
                     SGLRAGETRNVTIRFPTNVASMHQGAAASGDGADHANLIDDTEGTNWGSTGAPVQGRQ
                     VLVDLGGMRTFDRANVSAMLVPGQNRFVALRAFELHACTETAAITCDPSRDAGWTRIL
                     TSQDDAFPADNPRPVAPHLILRAFDVPRTTATHVLFRVVDNQCTGQTSFQGDQDDDPR
                     HETDCRIGTPLPERDTEVRAAELQLLSSTPQVSGAQQVE"
     gene            8355..8990
                     /locus_tag="VF058_01260"
     CDS             8355..8990
                     /locus_tag="VF058_01260"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6206963.1"
                     /translation="MLELDHVILGVADLPAASDRLRTELGLASVEGGRHVGVGTGNRI
                     VPLGRNYVEVLAVADPEEARAHPFGRWVLDYLGGRERFLGWCLRTDELDVIAGRLGLE
                     IESWSRERPDGAVLRWRMAGLDAAMEDPSLPFFMQWGIDDDDLYPGRSPAAHAAEPEG
                     FAWVEVAGDAERLADWIGPAELPIRVVEGDRGVTAAGILLGGSEVVLRPAP"
BASE COUNT         1206 a         3143 c         3439 g         1326 t
ORIGIN      
        1 agcgcgggcc cagccgctcg gtgaggtggt gggcggcgag gcggtaggcg aggacctgat
       61 cgcggtcgac ctcgggcacc gggacgacgc tacccaggtc ccctcgatac cgcaaccgag
      121 ttctctgagc cctcccggtc acatcggccg gatggagtcg gaacacgctc gtggtcgcgg
      181 agccgagtcg agcgcgcctc acaggaacgg gggaagctga cggcatgcac ccggtcttcg
      241 tccggaggca ggtcgagatc cgcgagtggc tggagcggaa caaggacacg gcggcggagc
      301 tgaggctgag catcctcctc cccttgaccc gaggaactca accaccgtag aattcatcgc
      361 acgttgagct gagggagggt gggatgtggg cgatcgcggt caaggagttc cgtcagctgc
      421 ggcgcgaccg gcggaccgtg gggctgatgg tgatggcgcc gctgctgctg ctgatcgtct
      481 tcggcttcgc ggcgaggttc gacgtcgagg aggtctcgac gatcgtggtg ggggagggct
      541 ccgaggcggc gcgggggcgt ctccccgagc tgttcgaggt ggtggagacg atgcccggcg
      601 gccgggccga ggccgtcgag ggcctgcggg aaggccgggc cgccgctgcc gtggtcgtcg
      661 ggccggagcc cgagatcctg ctggacgggg ccgagctctt ctccgcccag gcggcgctgc
      721 ggagcctggc gggagccggc ggtccgccgg ggggccctcg ccctcgagtg gaggtgctct
      781 tcaatcctgg gctcgagacg tcggccgtca tgatcccggc catcgccggg atgatcctcg
      841 tcttcgtggg cacgctgatc acgagcctgg gcgtcgttcg cgagcgtcag gccgggaccc
      901 tggagcagct ggccgtgatg cccttccgcc cctgggaggt gttcctgggc aaggtctccc
      961 cctacttcgt ggtggcggtc ttcgacctcg tgctggtcct gggggtgggg gtgctgctct
     1021 tcgacgtccc gttcgagggg tcgatcggga cgttcctgat cgggtccctg ctgttcctgt
     1081 tcgtgacgct gggcatgggc atcctggtgt cgagcgtcag ccagaaccag gggcaggcca
     1141 tccagctggc gatgatgctc ctgctgcccc aggtcctcct ctcagggatg atcttcccca
     1201 tcgagtcgat ggcggtgggc gtgcggtgga tcgcctacat cctgccgctg acttacttca
     1261 tcgagatcgc gaggggcgtg atgctgcgtg ccgccccgct cgatgccttg tggccgtacc
     1321 tggggttgct cgccgcgctc ggcctggtgg tgttcgggct cgccgtcctc cggtttcgac
     1381 gggatctggg gccggctcgg gccggcgccc gccggcccgc gccggccccc gaggccgcgc
     1441 gatgagctgg ggcgtctggg agatctcggc ccgctacggg gatcgtccgg cgctggaggg
     1501 ggtgaccctg gaaccccggc cggggtcgat cgccgccgtc gtgggcgcgg acggggcggg
     1561 gaagacgacc cttctgcggg tgctggctgg cgcccgcgac gccgacgcag gggaggtgcg
     1621 gcgtccggcg cccgagcgcc tgggcttcgt ctcccagggc tcgggggtgt acgaggacct
     1681 caccgtcgcg gagaacctcg cgttcgccgc ccgggcctac ggcgtggagg ggcacgacga
     1741 tcgcgcggcc gagctgctgg ccacgaccgg gctggcggag gcccgagagc gcctggggtc
     1801 gcagctgtcc ggagggatgc ggcagaagct gggggtggcg atggccatgg tccaccggcc
     1861 ggacctcctc gtcttggacg agcccacgac gggcctggac cccgtgagcc gggtcgagct
     1921 ctggacgatg gtggccgccg ccgccgccga gggggcggcg gtggtcgtct ccaccgccta
     1981 cctggaggag gccgaacggg ccacccacgt gctggtcctc gacgagggcc gaccgctcgt
     2041 gtccggctcg cccgaggagg tgctctcctc ggtccccggc gtcgtcctgg aggtccccac
     2101 ccggccggag gacgttccct cgtggaggag ggggaggacc tggcgagcgt ggatccggga
     2161 gggagacccg ccgcggggcg cccttcgggt ggcgcccgac atcgacgacg cggtgatggt
     2221 cgccgcgctg agcagggcgc ggccgtgacg acgctggcgc tggctcgagg cgtgacccgc
     2281 cggttcggcg aggtcgtggc cgtctcggac gcccgcctcg acctgggacg cggcgaggtc
     2341 gtcggccttg ttggcgccaa cggggccggc aagaccacgc tgatccgggt cctcctcggg
     2401 ctcctcccgc cgacctcggg ggaggtccgg ctgttcggcg aggctccctc ccggcgaacc
     2461 aggcgtcgcg tgggctacgt gccccagggc ctgggtctct acccggacct cacggtggat
     2521 gagaacctgc gcttcgcggc ggcggcctac ggcgccccgg cccccgtcac gcacgacgag
     2581 gtcgcgggcg tgggggactg gctggtgggc gagctcccgc tcggcctcca gcgtcgggtt
     2641 gcgttcgcag ccgcggtcgg ccacgagccc gacctgctcg tcctggacga gccgacctcg
     2701 ggtgtgggtc cgctggggag ggcccgcctc tgggacgaca tccacggggc ggccgaccgc
     2761 ggcgtgggcg tgctggtcac gacccactac atgagcgagg cggagcagtg cgaccgtctc
     2821 gtggtgctcg ccgggggtcg ggtcgtggcc gaggggaccc ttaaggagat cctgggcggg
     2881 ctcaccgtgg tccgtgtccg gaccgagcgg tgggaggacg cgttcggcag gctccgggac
     2941 gcaggccttc gcctcgggct cgtcggccgc tcgctgcgcg tcgtggaggc ccgcgtggag
     3001 gaggtggccg gatccctggc cggggcgggg atcgaggcgg aggtggccga ggccccggcc
     3061 accttcgagg aggccttcct catgctctcg gagggggcgt gatggtcagg accggcaggc
     3121 ggcccgggga gagcggcacg cgcgaggcga tcgtggaggc cgcgcgcgga gccttcgccg
     3181 agcacggcta cgagggcgcc acgatccggg ggatcgcggc ccgggccggc gtcgatcctg
     3241 cgctcgtgta ccacttccac ggatcgaagg aggagctgtt cgccgcggcg atgcggctcc
     3301 ccttccagcc gtccgagatg atcccctcgc tgctggaggg agaccccgac gggctgggcg
     3361 agcggatcct ccgcctgttc ctggtggtgt ccgaccagcc gggaggaagc ccgttcgtgg
     3421 ccctgatgcg gtcggccagc acgaacgagc gggcggcgcg catgctgcgc gagttcctga
     3481 cgagggaggt gatcgggcgc gtggcgaggg ccctgggggc tccggatgcc gagctccgag
     3541 cgaccctcgt ggggtcccac ctcgcggggc tggccatggc ccgatacatc ctgcggatgg
     3601 agccgctggc ctcggccgac cgggatacgg tcgtcgcggc cgtggccccc acgctccagc
     3661 gctacctcac cggggacctg gcctgagaga aaccctcggc ctacgcttgc cgtcgtaggt
     3721 cgccgtcgct acactctggc ccgccgtaac gaccgctgaa cctctcgaaa ggcgaaatcc
     3781 cgtgaaaacc cgcgtcggtt ggctgtgcgt gctcgcgatc gtgctcgtcc ttctgccctc
     3841 gttggctcac gccatctccc cggatctcgg agcagtcctc ggcgtcgagg aggtggtcgg
     3901 cgtcccgtcc cccaccgatc tgcgggggag ccatccgacc gaggcgatgg gctcgggcgg
     3961 aggggccctc ctctcgcccc ccagggtcgt gcctcgcagc gagtggggag ccgacgaagg
     4021 tctccgcttc cggggtcggc gcgcgaagcc cgcgttcctc ccggtgcgca agctcatcgt
     4081 ccaccacacc gtgaccaggc gctgggagcc cccgaggagg gcgatccgcc ggatctaccg
     4141 gtcgcacacg ctgtcgcgtg gcttcgccga catcgcgtac cactacctga tcgctcccga
     4201 cgggagggtc ttcaagggcc ggtactcggg ccccccgggg acccgccgca gggatcgccc
     4261 ctgggccgac cgggaccggc ggttcgccgt gcggggagcg cacgccctgg ccgcgaacga
     4321 cgggacggtg ggcgtcgccc tcatcggcaa ctacgagaac gccccgctgt ccctgaaggc
     4381 cagggggtcc ctggtccggc tgctcgcctg gctctcggat cggtacggga tcgatcccct
     4441 cgggtcgtcg aggtacccga cgtggggagg gcggatggtc cgcacgaggg acatcgccgg
     4501 gcatcgcgag tacgggcgga cggcctgccc gggggaagag gtccggaggg agctccccgc
     4561 gatccgccgg gaggtggtcg ggcgtctcag gcaggcggcc cgggggagcc ccgacgtcca
     4621 cggcccgctg atctaccgtg tccgcgtacc caaggtcggg cgacgggggg cgatcctgcg
     4681 ctggaccacg aacgagccgt cgaggaccgc cgcgtcggtc agggcccagg ggagctcgag
     4741 aggacggacc accgtgcgtc cggggctcac gaggcatcac accgtgcgcc tccgtccgct
     4801 ggatcggggc acgacctacg tgttccggct gggcggggcg gatgccggcg ggaacgtccg
     4861 gtggagcgcc ccgcactcgc tccgcaccaa gccctgacag tccggaccgt ccacatcgtc
     4921 acgggtgggg ccgtctctca gctcctgcgc accttcttgc cagggatacc cgttccagtc
     4981 gacgcgagga cttacgccgg acacgtcctc agcccgatag gacgcttctc gcaagggccg
     5041 aaggcgggga cgagcgacgc gctccctgcc gattggacat tctccgcgtg ccacgctata
     5101 tgagggcggg gctcgcgccg ggacgccgct cgacccgtaa gggaggagcc atggcgccct
     5161 accatcgttc cgcttcttcg acccggcgcc gacgccgagg tgtcgtcgcc tggttggtgg
     5221 cggtggtgct cgtggtcccg acgcaggcct cggcggtcgt gctgatcgac ggcctcggcg
     5281 gcggcgagcc ggagccgctc gacgcgcgaa ccgccgtcct gcagccgacg gccgaccagc
     5341 tcgccgccgc cgaggccctc ggggcgaccg tccgatggaa ccgcttcggc acgccgcagt
     5401 cgctgatccg gtacggaggc ccgctggcca gtggcgtccg gggcgccgac gcggagaccg
     5461 ccgcccgcgg ctggctggcc accaacgcgg gcctgttcgg gctcgacggg accgacgcgc
     5521 tcgagctcga cgcagccgtc ccgctccggg ggaccgacgc ccaggtcctg gtcttccgcc
     5581 agcgaatcga cggggtcctc gtatcgccgg acggcatcct gaccctcggc gtgaagggct
     5641 cggcgcggag cggcttcgac gtcgtccacg cctcgtcctc gctcgtgggg cgggacgccc
     5701 tcgagggcga gcgagagctc acggcgcgag aggcgctggt cgaggcggcg tccgacgtgg
     5761 gtgccgacct cgaaaccggg gacctccgtc gggccggcac gagcgcgggc tggagggtgt
     5821 tcgaggccga ggccctcgag ggagggtccc agctcgtccg ctcggtcgcc ttcccgatgc
     5881 cgagaagggg cgtcgttccc gcctacgaga ccgtggtgtc gccggaggtc gatgaagggt
     5941 tccgcacctt cgtgcacgcg gagacggggg aggtcctctt ccgcgagagc gtggtccacc
     6001 acgcggccga cgacccggag tgggagacct tcccctccta tcctcggacc actccgctca
     6061 accggccccc cttcaattac ccgagcgcgg acacgcgaca gctctggtgc tgggaaggcc
     6121 tgccggggtg ccgggtgcag gccgaggacc gttcgcccca tccggggacc ccgtgggacg
     6181 tggaccccgt gacggggacg cccaccttca cgacgaccgg gaactacgca cacagccagg
     6241 agaactggtt cgacttcctg ttccctggac ccaccggcta ccagcccacg agctccaccc
     6301 gcacgtacag gttcccctgg acgaaccagt ggttcgagga gcggtgcaac ccggacgtgc
     6361 tcgtggtggg gtccgggaac gacatcgacg cggccactac cgcgctgttc gtgggccaca
     6421 accggatgca caactgggcg taccaccttg gattcaccga gcacgcgtgg aacgctcagc
     6481 tgcacaattt cggcgtgggg accgcggagg gtgatcccct gatcgggatg gtgcaggcgg
     6541 gcgccgtgtc acacggtccc cccgtttacg ccggcaggga caacgccttc atgcggactc
     6601 ttccggacgg gacgccgtca cagtccgcga tgttcctgtg gcagccgatc cccggagcct
     6661 tctacgcccc ctgcgtggac ggcgactacg acatggcggt gatcggacac gagtacacgc
     6721 acatgatcga gaaccgaatg atcggcaagg ggaaccgtcg gatggggttc cacgccggcg
     6781 cgatgggcga gagcgtgtcc gacttcatcg cgatggagca catgaacgag tacggcgacc
     6841 tccccggcgg tgacctggcg tgggcagtcg ggccctacgt gaccgggaac cccgtgcggg
     6901 cgatccggaa ctacaacatg tcgttcccct ccgcgggggc gttccccgag gagggccggt
     6961 acccgcgcgt caacccgctc aacttctcgg ccgtcggata cgacatcgtg ggcccgcagg
     7021 tccacgccga cggggagatc tggagcgcga cgaacttcga catccgcgag ctgttcgtcg
     7081 cccgctacgg cggcggcggc gctcagctcc aggagcagtg cgcgaacggc gagcgcccgg
     7141 cgagtcagtg cccgggcaac cggcgctgga tccagctcgc cttcgacgcg ttcctgctga
     7201 tgccggtggc gcccacattc ctcgacgccc gcgacgccta cctcgccgcg gacatgctgc
     7261 ggttcggcgg cgcgaaccag gatctcctct ggcaggcgtt cgcgcgccgg ggcttcggcg
     7321 agcacgccag cgtgaccgga acccaggaca ccgaaccggt gccgagctgg gagtccccgc
     7381 tccacgagga agccacgctg gtcttcgacg cggtggcgaa ggacgagggc ggcgcaccca
     7441 tcgaggcgga cgtcttcgtg gggcactacg aggcccgcgt cacgccggcg gcccggacgg
     7501 ccgcggggat gaacgagctg cgcatcctcc ccgacacgcc gggtggtcgg caccgcgcgt
     7561 acgagttcac cgcccacgcg gagggctacg ggcacgtgcg cttccgggtc tcgggcctga
     7621 gggccggtga gaccaggaac gtcacgatcc ggttccccac gaacgtcgcc tcgatgcatc
     7681 agggcgccgc ggcctccggc gacggcgcgg accacgccaa tctgatcgac gacaccgagg
     7741 gcacgaactg gggcagcacg ggggcgccgg tccagggccg gcaggtcctc gtggacctcg
     7801 ggggtatgcg gacgttcgat cgagcgaacg tcagcgcgat gctcgtgccc ggccagaacc
     7861 ggttcgtggc gctccgcgcg ttcgagctgc atgcctgcac ggagacggcg gccatcacgt
     7921 gcgacccgtc ccgcgacgcg ggatggacga ggatcctgac gagccaggac gacgcgttcc
     7981 cggcggacaa cccccggccg gtggcgccgc acctgatcct gcgcgcgttc gacgtgccga
     8041 ggacgaccgc gacgcacgtc ctgttccgcg tggtcgacaa ccagtgcacg ggccagacct
     8101 ctttccaggg agaccaggac gacgacccga ggcacgagac cgactgccgg atcgggacgc
     8161 cgctgcccga gcgcgacacc gaggtgcggg cggccgagct ccagctcctt tcgagcaccc
     8221 cgcaggtgtc gggggcccag caggtcgagt aggtcgccga gggagggccg gcccgcccgc
     8281 cggccctccc tccgctcgtc cgaaggtgag ccgcgaccgc cccggctcgg gccgcccgtg
     8341 gtaggttccg cgggatgctc gagctcgatc acgtgatcct cggcgtggcg gacctcccgg
     8401 ccgcgtcgga ccggctccgg acggagctgg ggctcgcctc ggtggaaggg ggccgtcacg
     8461 tcggcgtggg caccgggaac cgcatcgttc cgctcggccg gaactacgtg gaggttttgg
     8521 cggtggccga tcccgaggag gcgcgggccc accccttcgg gcggtgggtc ctcgactacc
     8581 tgggtggccg cgagcggttc ctcggctggt gcctgcgcac cgacgagctc gatgtgatcg
     8641 ccgggcggct cggcctcgag atcgagtcct ggtcccggga gcgcccggac ggagccgtgc
     8701 tccgctggcg gatggcgggc ctcgacgcag ccatggagga tccctcgctg ccgttcttca
     8761 tgcagtgggg gatcgacgac gacgacctct acccgggacg gtcgcctgcg gcccacgcgg
     8821 ccgagcccga ggggttcgcg tgggtggagg tggcgggcga cgcggagcgc ctcgcggatt
     8881 ggatcggccc ggccgagctc ccgatccgag tcgtggaggg ggaccggggc gtcaccgccg
     8941 cggggatcct gctcggcggg tccgaggtgg tcctcaggcc ggctccctga ccgggcgggc
     9001 taccgagcat cgtcgaggac gcccagggcc tccccacaga agcggatcga gcgctcgtcg
     9061 atcggatcga ggacgaggat gagctcgtcg gcgccggcct ccgcgatctc gcgg
//
LOCUS       DASUTW010000036         4692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_529284, whole genome shotgun sequence.
ACCESSION   DASUTW010000036 DASUTW010000000
VERSION     DASUTW010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529284"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="VF058_01265"
     CDS             complement(<1..179)
                     /locus_tag="VF058_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018105457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX6206964.1"
                     /translation="MSSTSAATQHLRHLARLRRVRDRIDREYARPLDVEELARGAHMS
                     AGHLSRQFRRAFGESP"
     gene            complement(240..1355)
                     /locus_tag="VF058_01270"
     CDS             complement(240..1355)
                     /locus_tag="VF058_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015332942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEX6206965.1"
                     /translation="MDETLLTIGIEEEFQIVDEGGELRANIDAVMARAEQKPELEPED
                     VKPEMLQSVVEVGTKVCSNIKDARREVERLRGQLVEILGEEDLRLASAGTHPFSHWKD
                     QPVTQKERYLSLEEEMQDVVRELLIFGLHVHIGIPDRDLRIDIFNEARYFLPHLLCLS
                     TSSPFWLGRNTGLKSYRSILWSRFPRTGVPPDFPTWDDFDHYVNLLTKTQSIPDGSKI
                     WWDLRPHHVYPTLEFRVCDAATKIDETICIAAIIQAFVAKLLKLRKQNLGFRKYPGYL
                     IRENKWRAVRHGLEGKLIDFGKQEEVPERDLVLEILDFVDDVVDDLGSRSEVNYARQI
                     LAEGTSAERQLRVYEETNDLRSVVDHICDETRQGLPG"
     gene            complement(1387..3708)
                     /gene="lnt"
                     /locus_tag="VF058_01275"
     CDS             complement(1387..3708)
                     /gene="lnt"
                     /locus_tag="VF058_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX6206966.1"
                     /translation="MSELLPRQEQEPARRPEADALQGLRLVSAPVRLPLALPAAALSG
                     LLLWMGFAPVDLDAIGFVALVPILWAVRGARARRGALLGLVFGLTYFGILMSWLLPLT
                     ILGWSVLVLGSAGWMALLFAAGAAVWRDDRPIVSALAFGAVWVSVEWMRGVLPFGGFT
                     WGGLGVTQHDNPLLLPLASVAGSWGISFVLAAVNALVLEALERVRAGWGRPARLLGIA
                     VALALLPALIPTAASDGPPVEIAVIQGNVPRDVAARDRLIVDRIVAENHARLHRTLAE
                     DPPDLAVWPENALDQDPRRDPSLAALVEGAISSVGSPTLVGAITFEDDGALFNENLLY
                     TGRGEIVGRYVKNHLVPFGEYVPFRARLEGFVPDIERVREDLTPGTEPGRFPVPGAPG
                     ASFASVICFENAFPDLVRQWVTADMGLLVISTNNSTFGTGPAARQHLALSEMRAVETG
                     RWVVHGALTGISGIVDPGGRVRQRTPLFEPAVLRASIPQGEGRTVFNVIGGWLPSVFL
                     LAASLALLAPRRPRRRVVPPLTASPRVCVVIPTYDEAATIEEVVGGVRAVGERVEVLV
                     VDDASPDGTAEIVRGLKGEGRVRLLEREGKRGLATAYLEGFRSALDEGHDVIVEMDAD
                     LSHDPGDLRALLAGARSHHLTIGSRYVPGGSIPDWGLGRRILSRWGNLYARLLLGLPV
                     ADATSGFRVYRADALGELIDPPPSADGYAFQIELAYRAWRLGMSVGEVPITFRDRTHG
                     RSKLSRRIVVEALWKVLAWGIRDRLLRRRPPSA"
     gene            complement(3630..3938)
                     /locus_tag="VF058_01280"
     CDS             complement(3630..3938)
                     /locus_tag="VF058_01280"
                     /inference="COORDINATES: protein motif:HMM:NF025204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 family protein"
                     /protein_id="HEX6206967.1"
                     /translation="MEDAMDDKVETATEEEDEAPAEAGDEESLDELIAKKTSEDAAED
                     ESEEETVLKLEPEERVGGSLPSNVQPQQSSEFTCRNCFLVKSKSQLADRKRMLCRDCA
                     "
     gene            4178..>4692
                     /locus_tag="VF058_01285"
     CDS             4178..>4692
                     /locus_tag="VF058_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206968.1"
                     /translation="MATEGGPTLAAVDLTEATGLEDAASFVVDLLRRPASEIRVLEGQ
                     LGLEEDLVVEGHPDAFDLSPVEPADAGTVWAVDGGSCTLADGRSFQVVGTRAARVRFR
                     KGRTDLVETPPLSVRAVSQAEMRRIGREILTELCGREPERLPDMPRPVDALREWGEWA
                     EVRRTVSEASAG"
BASE COUNT          690 a         1658 c         1684 g          660 t
ORIGIN      
        1 ggcgactcgc cgaaggcgcg ccggaactga cggctgaggt gcccggccga catatgcgcg
       61 ccgcgcgcga gctcctcgac gtccagcggc cgggcgtact cccggtcgat ccggtcgcgg
      121 acgcggcgca gccgcgcgag gtggcggagg tgctgcgtcg cggcggatgt gctgctcacc
      181 ggcgcgatgg tatcaagtcg gaccggtgtc tcccccgacc cccgcgacgc gtccggggct
      241 cacccaggga ggccctggcg cgtctcgtcg cagatgtggt ccacgaccga ccgcaggtcg
      301 ttcgtctcct cgtagacccg aagctggcgc tcggcgctgg tgccctcggc caggatctgc
      361 cgcgcgtagt tgacctccga gcggctgccg agatcgtcta cgacgtcgtc cacgaagtcc
      421 aggatctcca ggacgaggtc ccgctcgggc acctcctcct gcttcccgaa gtcgatcagc
      481 ttcccctcga gcccgtggcg gaccgcccgc cacttgttct cgcggatcag gtagccgggg
      541 tacttccgga accccaggtt ctgcttgcgg agcttcagga gcttggccac gaaggcctgg
      601 atgatcgcgg cgatgcagat cgtctcgtcg atcttcgtgg cggcgtcgca cacgcggaac
      661 tccagcgtcg ggtagacgtg atggggccgg aggtcccacc agatcttcga gccgtcgggg
      721 atgctctggg tcttcgtcag caggttgacg tagtgatcga agtcgtccca cgtggggaag
      781 tcgggcggca ccccggtcct ggggaaccgc gaccacagga tcgagcggta ggacttcagc
      841 cccgtgttcc gccccagcca gaacggcgag gacgtggaga ggcacagcag gtgcgggagg
      901 aagtacctcg cctcgttgaa gatgtcgatc cggaggtccc tgtcggggat cccgatgtgc
      961 acgtgcaggc cgaagatcag gagctcccgc accacgtcct gcatctcttc ctcgaggctg
     1021 aggtagcgct ccttctgggt gacgggctgg tccttccagt ggctgaaggg gtgggtgccg
     1081 gcagaggcca ggcgcaggtc ctcctccccc aggatctcca ccagctggcc ccggagccgc
     1141 tcgacctcac ggcgcgcgtc cttgatgttc gagcagacct tcgttccgac ctcgacgacc
     1201 gattggagca tctcgggctt gacgtcttcg ggctcgagct ccggcttctg ctcggcgcgg
     1261 gccatcacgg cgtcgatgtt ggcgcgcagc tcgccgccct cgtcgacgat ctggaactcc
     1321 tcctcgatcc cgatggtcag gagggtctcg tccacgggga tactcgaccg tacggagggc
     1381 gcggcgtcaa gcgctcgggg ggcggcggcg caacaagcga tcccggatgc cccaggccag
     1441 gaccttccac agggcctcga ccacgatcct gcgggagagc ttggagcggc cgtgcgtacg
     1501 gtcccggaac gtgatgggga cctcccccac cgacatcccg aggcgccacg ctcggtaggc
     1561 cagctcgatc tggaacgcgt aaccgtcggc gctcggcggt ggatcgatca gctctccgag
     1621 cgcgtcggcc cgatagacgc ggaagccgct cgtggcgtcg gccaccggga gccccagcag
     1681 cagccgcgcg tagaggttcc cccagcgcga aaggatgcgg cgccccagtc cccagtccgg
     1741 gatcgatccg cccggcacgt agcggctgcc gatcgtcagg tggtgcgagc gggctccggc
     1801 cagcagcgcg cggaggtctc cggggtcgtg ggagaggtcg gcgtccatct ccacgatcac
     1861 gtcgtgcccc tcgtccagcg ccgagcggaa cccctcgagg tacgcggtgg ccagccctcg
     1921 cttcccctcg cgctccagga ggcggacgcg cccctccccc ttcaggccgc ggacgatctc
     1981 ggccgtcccg tcgggtgagg cgtcgtccac gaccagcacc tcgacccgct cccccactgc
     2041 ccggacgccc ccgacgacct cctcgatcgt ggccgcctcg tcgtaggtgg ggatgaccac
     2101 gcagacgcgc ggcgacgccg tgagcggcgg gacgaccctc cgccggggac gacggggggc
     2161 gagcagcgcc aggctcgccg ccagcaggaa gaccgagggc agccagcccc cgatcacgtt
     2221 gaacaccgtc cgtccctcac cctgtgggat cgaggcccgc aggaccgccg gctcgaagag
     2281 cggcgtgcgc tgccgcacgc ggccgccggg gtccacgatc ccgctgatgc cggtgagggc
     2341 gccgtggacc acccagcgcc cggtctcgac agcccgcatc tcgctgaggg ccaggtgctg
     2401 gcgggcggcc ggcccggtcc cgaacgtgga gttgttcgtg gagatcacga ggagccccat
     2461 gtcggccgtc acccactgtc gcacgaggtc ggggaaggcg ttctcgaagc agatcaccga
     2521 ggcgaaggag gcccccgggg cgcccgggac ggggaaccgg ccgggctcgg tccccggcgt
     2581 gagatcctcg cggacccgct cgatgtccgg gacgaacccc tccagccggg cccggaacgg
     2641 cacgtactcc ccgaagggca ccaggtggtt cttcacgtac cgccccacga tctcgccccg
     2701 gcccgtgtag aggaggttct cgttgaagag ggccccgtcg tcctcgaagg tgatcgcgcc
     2761 gacgagggtg gggctgccca ccgaggagat cgcgccctcc accagcgccg ccagggatgg
     2821 atcgcgccgc gggtcctggt ccagggcgtt ctcgggccac acggcgaggt cgggagggtc
     2881 ctcggccagg gtccggtgga ggcgggcgtg gttctcggcc acgatgcggt ccacgatgag
     2941 acggtcccgg gccgcgacgt cgcgcggcac gttcccctgg atcacggcga tctcgaccgg
     3001 agggccgtcc gaagcggccg tagggatgag cgccgggagg agcgcgaggg ccaccgcgat
     3061 ccccagcagg cgggcgggcc tcccccagcc ggcgcgcacc cgttccaggg cctccagcac
     3121 cagggcgttc accgcggcca ggacgaacga gatcccccag gagcccgcca ccgaggccag
     3181 cggcaggagc agcgggttgt cgtgctgggt cacccccagg ccgccccagg tgaagccgcc
     3241 gaacggaagc acgcctcgca tccactccac ggacacccag accgcgccga aggccagggc
     3301 cgacacgatc ggcctgtcgt cgcgccagac ggccgcgccc gcggcgaaca gcagggccat
     3361 ccatcccgcg gagccgagga cgagcaccga ccaccccagg atcgtcagcg gcagcagcca
     3421 cgacatcagg atcccgaagt aggtgaggcc gaagacgagc ccgagcagcg ctccccgccg
     3481 ggcccgagcg ccacgaaccg cccacaggat cggcacgagc gccacgaatc cgatcgcgtc
     3541 gaggtctacg ggggcgaacc ccatccagag gagcaagccc gagagggccg cggcgggcag
     3601 agcgaggggg agacggacgg gggcggagac taggcgcagt ccctgcagag catccgcttc
     3661 cggtcggcga gctggctctt gctcttgacg aggaagcagt tccgacacgt gaactccgac
     3721 gattgctggg gctggacgtt ggagggcagg ctgccgccga cccgctcctc cggctcgagc
     3781 ttcagcaccg tctcctcctc ggactcgtcc tcggcggcgt cctccgaggt cttcttggcg
     3841 atcagctcgt cgaggctctc ttcgtccccg gcctcggccg gggcctcgtc ctcttcttcg
     3901 gtagcggtct cgaccttgtc gtccatcgca tcctccatca aggctgcgcc attctacccg
     3961 ccggtcgtcg gatggaagcc ggatccagga cggaaagcga agggccaaga gacctttggg
     4021 gccgcagcgc cggggcgccg cgttctctac tcagcctctt ggcccgtcgc cgcgccggtg
     4081 gcccggcgct gcggcccaca atctagtcgg cgccccgagg ggtgtcaacc ggacgggctg
     4141 cgcgatcgcg tcggtggggc gggatatcct gacgccgatg gcgacggagg gaggaccgac
     4201 gctggccgcc gtggatctca ccgaggccac gggcctcgag gacgcggcgt ccttcgtggt
     4261 cgatctgctt cggcggccgg cgagcgagat ccgggtcctc gagggccagc tgggcctcga
     4321 ggaggacctc gtcgtggaag ggcatccgga cgccttcgac ctctcccccg tcgagccggc
     4381 cgacgcaggg acggtgtggg ccgtggacgg cgggtcctgc acgctggcgg acggccgctc
     4441 gttccaggtg gtggggacga gggccgctcg cgtgcggttc cggaaggggc ggacggacct
     4501 cgtggagacc ccgccgctct ccgtgcgggc cgtctctcag gccgagatgc gccggatcgg
     4561 acgcgagatc ctcaccgagc tgtgcgggcg cgagcccgaa cggctgccgg acatgccgcg
     4621 ccccgtggac gctctccggg agtggggcga gtgggcggag gtgcggagga cggtctcgga
     4681 ggcgtcggcc gg
//
LOCUS       DASUTW010000037        16929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1566791, whole genome shotgun sequence.
ACCESSION   DASUTW010000037 DASUTW010000000
VERSION     DASUTW010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 16929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16929
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566791"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..493)
                     /locus_tag="VF058_01290"
     CDS             complement(140..493)
                     /locus_tag="VF058_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX6206969.1"
                     /translation="MLGRLLRRFAESDEDRLANEVRAWADSMPETTRISDVRERARVR
                     LAGVVRRITLHPGPEGAESLSAVLSDGTGEIVARWTGRGEIGGLRLGSRVLLEGVAAR
                     ERGRLRIVNPSYEFA"
     gene            complement(498..2771)
                     /locus_tag="VF058_01295"
     CDS             complement(498..2771)
                     /locus_tag="VF058_01295"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012855125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX6206970.1"
                     /translation="MVVLAQEGGPISAEFGSFEDAVLWIILGISVLALVVAYVLRRGV
                     LAAPEGTEKMREIATAIQQGSRAYLNRQFRTVGMFLVVLTIAIFFLLPVPENAETSAI
                     AIRLGRSVAFILGAGFSAVTGFAGMWLAVRGNVRVANAAKETGLKRAVVIAFRTGGVA
                     GMFTVGLGLLGAVVILLIYKQDATSVLVGFGFGGALLAMFMRVGGGIYTKAADVGADL
                     VGKVEQGIPEDDPRNAATIADNVGDNVGDCAGMAADLFESYEVTLVASIILGAAAFAD
                     SPEGAIVGVMFPLFVRAIGVITSIVGIYAVTPRSDTEHGMRAINRGFFISAVLSAAVV
                     YFVADAYVNDLRVFWAVVIGLLLASVIHVLTEHYTSTKRRPVKEVAESSQTGAATTIL
                     SGFSVGLESTVWAILVIAGTIFSAYLLGNDIAERLYFISLTGMGMLTTVGVIVSMDTF
                     GPVADNAQGIAEMSGEFEGRPAEVLEGLDAVGNSTKAITKGMAIATAVIAATSLFGSF
                     EEALKEAGYSFIGIRVDQPEVLIGLLVGGAVSFLFAALAIRAVGRAAAQVVVEVRNQF
                     RDHPGIMDGTEKPDYGRVVDICTRTSLRELLTPGLLAVLTPIIVGFAFEAEALGAFLA
                     GVILTGQLLAVMLSNSGGAWDNAKKLVEEGLYGGKGSESHKATVIGDTVGDPFKDTAG
                     PALNPLIKVMNLVALLIAPLVVTMADDTVARIAIAASGAVILGLAIWHSKRQRGPVLV
                     SSEPPSPEAATVTTESG"
     gene            2904..3560
                     /locus_tag="VF058_01300"
     CDS             2904..3560
                     /locus_tag="VF058_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206971.1"
                     /translation="MPRRAHAASGRALPWILVGLLVLVASGAGAWALLRDGAPEDEED
                     LPVPDLVWTIGRVRSTSVDADASQGDLEEARDAIADLLDDLYTTAFVDPAKWEGGAFP
                     ELGSYFAGSAAARAERDRDGLTLGEDAARIERVEPTRATLRLTFLVDPQAAPYAAVAT
                     AGFRADGRTTDGGAVAIVHQGRYMVRRIEGEWRIIGYDVEGSLESVADPGPGTPGATP
                     "
     gene            3557..4495
                     /locus_tag="VF058_01305"
     CDS             3557..4495
                     /locus_tag="VF058_01305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX6206972.1"
                     /translation="MRRGAALLVALVVGLGAWVAVAAFLSEPRPAGAQTPAVEISRAQ
                     DARYTPSLGGNRPIFILAIGSDARPDEQVDRRLADSLHIVAVNPRQGSASILGFPRDS
                     YVPIPGFGEAKINESLYNGGPELVVETVEGLTGIQMDYWVLTSFRGLIGLVNGIGGLE
                     IDVPYPMDDFYSGAQFEAGEQVIDGSQALAFARNRHDTPEGDFSRSENHGRLLLAALE
                     QLRSQVRRDPTVLFQWIAVGLRNVQTDLSFDEVFDLMMTALSIDPESVTNAVVPGGTG
                     SAGAASVVFLSGDAPAIFEDLGDDGILQNPPPPPGG"
     gene            complement(4492..5493)
                     /locus_tag="VF058_01310"
     CDS             complement(4492..5493)
                     /locus_tag="VF058_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX6206973.1"
                     /translation="MSVAAAEQAQARFASFDVPMWVWAVLIGAIVTLLLIDLLIVHRT
                     PHVISFKEAAVESAVWVAIGISFTFVLLWWQGGQAAGEYFAGYLIEKSLSIDNVFVWA
                     VLLTYFHVPREYQFRVLFWGVFGALVMRAIFIFAGVALLERFDWVLYVFGAFLVYTAF
                     RLARHSGAEVHPERNPVLKLVRRVIPTTHYYDGQRMLTRQEGRLMATPLLVVLILVEA
                     TDVLFAVDSVPAILAVSREPFIVWSSNAFAIMGLRALYFLLAGMADRFRYLNVGLGVV
                     LGFVGVKMLLADVYHVPVWASLVVIAAVLTATIWLSLRVSGREEEEEEEEAPDPRSR"
     gene            5567..7816
                     /locus_tag="VF058_01315"
     CDS             5567..7816
                     /locus_tag="VF058_01315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6206974.1"
                     /translation="MDPHDLLATLRSEAPDRLVHLRTLPERRPRLARLPDLPEPLPAR
                     LDNLGIEGLYPHQAEALELLEEGRNVVLSTGTASGKSLVYNVHLARTLLRDPRSTGLY
                     LFPTKALARDQLRQVRGLRLPQIKASVYDGDTPRAERPLIRRTANLILSNPDMLHASL
                     LPDHARWADFLLRLAVVVVDEAHVARGVFGSHVAMVLRRLRRLVAKYGGDPRFVLASA
                     TIGNPADLAERLTGVPFAQVTEDASPAGEKLFALWNPPLVDDEEGGRKGAVSEAAGLL
                     ARLVDRDERTIAFARSRRAAELLAEIARREVGAARKGRIKTYRAGYLPEERRRLERAL
                     SEGELLGVASTNALELGIDIGSLDAAILAGYPGTRASMWQQAGRAGRRSSASIAFLVA
                     QDDPLDQFLVSHPQDLFDKPPEDAVVDPTNPYVLAPHLACAAREHPLAPEELSFFGDA
                     APDAVASMLEEGRLVERRGLLYHRGGPSPHREVDIRSASGSLYLIVEVDTGAVLGNVD
                     ESRAFFQVHPGAIYLHQGEQYEVVTLDRERKAALVRPSDADWFTQSRDVTDIEVLRVR
                     AETKVGSTPAFFGDVRVTTQVVGFQRRLHITGEVLGVVDLDLPPQTLVTRALWWAVPD
                     ETIERAGVTPSALPGAAHAAEHAAIGLLPLVATCDRWDIGGVSTAHHPDTGSCAIFIY
                     DGYPGGAGIAERGFASGERLLRATLETIRACPCQSGCPSCVQSPKCGNGNEPLDKDAA
                     AALIEEMLA"
     gene            7813..8298
                     /locus_tag="VF058_01320"
     CDS             7813..8298
                     /locus_tag="VF058_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HEX6206975.1"
                     /translation="MIQIQIAVCGPGVATEEEGRWAEEAGRLLAEEGAVVVCGGMTGV
                     MDAAARGARQAGGLALGILPGRGLEGASEHLSAAVVTGMGEARNALVVRSADAVLAIA
                     GEYGTLSEIALALKMGVPVVGLRTWELAKGGEPVEAFVRAAGVPDAVREAMRLARERR
                     S"
     gene            8295..9107
                     /locus_tag="VF058_01325"
     CDS             8295..9107
                     /locus_tag="VF058_01325"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX6206976.1"
                     /translation="MKRRVVNALAKHVANPVMRRLAGRIPGWALLETTGRRSGRAHQI
                     PVGDGLRGDAFWIVAEHGRHADWVRNVEADPRVRIRVRGRWRTGTAHILEDDDPRERL
                     RRLGGVNPWVIRVAGTEPLTVRVDLDPAPPRPGSVLRDGLVAGLVAGAVAGAPSGFHA
                     LRHGYLLDLLRAPGALVAPRAGAEAQLALTVPVHAVISAGWGVVLASALPRRATALWG
                     AAGGVVIATLDIVVIGSRIPVIRRLPLWPQVADHVAFGTVAGGILAALRDPS"
     gene            complement(9104..10678)
                     /locus_tag="VF058_01330"
     CDS             complement(9104..10678)
                     /locus_tag="VF058_01330"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX6206977.1"
                     /translation="MATSNSGKRARRIRSALESVLPLERFGDERTILAGTGQNVVGMV
                     VAIVATFGTQVLITRALGASAFGIVTLATQGALVLGYFARFGMDMAAVRRVAIDVGRG
                     ERSRVRAVVMRASIISTVSSLIVAAAVFLLAEPIARSLAADAAGAATGAFRAAALAIP
                     FTALVQVYLGATRGLKIMRHTLYVFWMGQPLAWFALILAGWLVSRSVGVTTLAYTVSW
                     VGATAAAAWLWYRETRGFGGDPPLPGEVRDLMRYGAPRAPAALFSQLLFWTDVFVLAV
                     FASSREVGIYSAAARAGQVLLLFIISVSLMFSPFVADLHARGEREKLDRLYKQLTRWT
                     LAATLPVFIVLTAAPESALRLFGAEFGVGATALGILLLGQLVNVATGAVGFVLIMIGR
                     TGWDLLVYAASVAFDIGAAAFLIGGLGLGIEGAAIAGATTMALSKVARLWLVWRFMGM
                     QPFTRHYALLAVPLALGLGAALGAHALVAGASWPVDLAVTGGIGTLAYVAVLVAFGLP
                     PAERRVLARLVGAVRG"
     gene            complement(10662..12245)
                     /locus_tag="VF058_01335"
     CDS             complement(10662..12245)
                     /locus_tag="VF058_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6206978.1"
                     /translation="MARVLLIGWDGADWRILDPMLEAGLLPNLKALIDRGARGVLKST
                     VPNHSWSAWPSFLTGLEPSNHGVYDIFERDFRTRKQLPVTYRSIKERTILPDLAAAGV
                     ETIMTNVPLTFPPQRIQGKLISGGVLPKGRPHTHPEGLAAELEAAGKPFPINGMSWTT
                     FWNRPEPFLDEAMTLTRQRIDANEHLLETTDWRFASLVYVATDRVQHCLAKYVSPDHP
                     NYDTLSKEPLAERIRDVYRLLDEGLGRHLVHVGDDDLILFISDHGFQSCTRALHMDKF
                     LEHLGFLRFAASSAVFGPMQWGRVRALARRVYDLLGLHGKVSLPQSVNWRKSIAYTSV
                     RSTGEGVSINLAGREPDGTVDPADFESTREKVMDALSGFVDPATGRKPVKAIHKREEF
                     FQGRFADTAPDIIMEPAEGYSLTHAKTAIEDADWLSGDHRMDGVIAAAGPHVRTDAFE
                     RPALLVDVAPTTLAALGVTSSVKHDGQVLESVVGAERSTAAEAAASSETAEEHEDTGL
                     DADEAAEVEEHLRGLGYLE"
     gene            complement(12325..13020)
                     /locus_tag="VF058_01340"
     CDS             complement(12325..13020)
                     /locus_tag="VF058_01340"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HEX6206979.1"
                     /translation="MAREEQEPLYPSTVLSSDPEVSGVRSVGGLVLDAWRRRPVPRRV
                     LGTLSVFLLLGGLTMVFYPHLTNLYGDWRQSGLEASFAEPETREAYAARAVLPGEALT
                     KVEIPRLELVSIVVEGTSPGALRAGAGHYEGSALPCEVGNAVVAGHRTTYSKPFADLH
                     RLRPGDEVVLVTPVGRCVYQVAGSWVTTPDDVSVLADVRGASVLTLTTCHPPGSARER
                     LIVRAELVSSEVA"
     gene            13137..13697
                     /locus_tag="VF058_01345"
     CDS             13137..13697
                     /locus_tag="VF058_01345"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6206980.1"
                     /translation="MLEFAILGLLQERSMHGYQLKQRLADALGPFWQVSYGSLYPALK
                     RLQKQGAVEMVFPKAEVGRRKNVYRITEHGQELFAELGESTQEDSDDNGFRVRLAFFR
                     HLKPEARIGVMERRRAVLVDRLRELKDRLASYRERVDGYTLSLMRHGMDTTEHDIRWL
                     DDMIQAERRASRSGRKRRAPKKGVRA"
     gene            13694..14821
                     /locus_tag="VF058_01350"
     CDS             13694..14821
                     /locus_tag="VF058_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018349013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX6206981.1"
                     /translation="MSKVRLAIVGVGNCASSLVQGLEFYKDADPAEQVPGLMHVLFGP
                     YHVSDVEVVAAFDVDGKKVGRDVSEAIFAEPNNTIRFSDVPPTGVEVLRGPTLDGFGR
                     YYREVAEESDLEPVDVAEALRDAGTDVVISYLPVGSEQAARHYAQAAIDAGAGFVNCM
                     PVFIASDRAWAKRFGDARLPIVGDDIKSQVGATIVHRMLAKLFEDRGVVLDHTSQLNF
                     GGNMDFMNMLERERLTSKKISKTQAVTSQLRDGIPAEDIHIGPSDHVPWLHDRKWAHI
                     RLEGREFGGVPLSVELKLEVWDSPNSAGVAIDAIRSAKLAKDRGVGGPLVGPSAYLMK
                     SPPVQFSDDAARDMVEEFILGNGGAKDQDWTKFFDVQPQES"
     gene            14876..16336
                     /locus_tag="VF058_01355"
     CDS             14876..16336
                     /locus_tag="VF058_01355"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6206982.1"
                     /translation="MSGRPRLPGLRDLSGRRGFRRLIASQVTSQAADGLYQIAIAAVL
                     IFGVDAAETPGQVTKVLAVTLLPFSLVGPFTGPFIDRFSRRSILVGTSVARAALTFAL
                     IPATGWPEWIILALVVANMSVNRFFHATKSSALPTLVPRHRYLLANTVSSVGGMVFGL
                     AGAVVGGPVADAVSAEVVILAAGVLMTVAAVLATTVPLPPGERKGLTGLLGELRDDAR
                     DVRDGLRLLLGTPRAVYAISSIWSIRGLIGFLLLAGIVLLRRRFGLETTGASLLLGAV
                     GIGGFLGALLVTPAARRLGDARVAPLAYLVAGIAVLALGAVPAWPALVTLVAVGGAAM
                     AMTKITADTMVQRSIPDSHRGRAFAIYDIGYNGVFVLAALIPTLLIGLLGDIGIVFLA
                     GTLAVIGAGILALASRRLGGLEVRAYAGGRADETPREVVIDGTRLMVTGVERSWHEER
                     AGRRLLVFVLRLEDGRRLQLSRGEEWRIDRFLGEPG"
     gene            complement(16333..16719)
                     /locus_tag="VF058_01360"
     CDS             complement(16333..16719)
                     /locus_tag="VF058_01360"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HEX6206983.1"
                     /translation="MRRGGALPVLAAVAGWLAWRRPFRVVVEGVSMASALRPGDQLLC
                     VRRRRIRRGDVVVVRPPSHDVEMVKRVTGLPGDPMGARTLGEDEYQVEGDDPLRSTDG
                     RSFGPVARRDILGVAVLRYLPDPRLL"
     gene            16806..>16929
                     /locus_tag="VF058_01365"
     CDS             16806..>16929
                     /locus_tag="VF058_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Ni]"
                     /protein_id="HEX6206984.1"
                     /translation="MLSRLLRPRRVVRAHCDLPCGVYDPAQARIEAESVQKIQEK"
BASE COUNT         2547 a         6159 c         5892 g         2331 t
ORIGIN      
        1 gagggccacg tcgtcgtgcc gcagccggag acgcggctcg tggccggcga cgagatcatg
       61 gctatctcca cgctggaggc cgaggacgac ctgcgcgagg cgatcctcgg gcccaccccc
      121 cactccgagg cgcgctgact caggcgaact cgtagctggg gttcacgatc cggaggcggc
      181 ctcgctcccg cgccgccacg ccttccagca ggacgcgcga cccgagccgg aggccgccga
      241 tctccccgcg ccccgtccac ctcgcgacga tctcccccgt cccgtcggag agaaccgcgg
      301 acagcgattc ggctccctcg ggcccggggt gcagcgtgat ccggcgcacg acgccggcca
      361 gccgcacccg ggcccgctcg cggacgtcac tgatcctggt cgtctccggc atggagtccg
      421 cccaggcccg gacctcgttc gccaggcgat cctcgtcgga ctccgcgaaa cggcggagca
      481 gccgaccgag cacggcctca gccgctctcg gtggtgacgg tcgcggcctc cgggctcggc
      541 ggctccgacg agaccaggac cggcccccgc tgccgcttcg agtgccagat cgcgaggccc
      601 aggatcaccg ccccgctcgc cgcgatcgcg atcctggcga cggtgtcgtc cgccatcgtc
      661 accacgagcg gcgcgatcag cagcgccacg agattcatca ccttgatcag cgggttgagg
      721 gcgggcccgg cggtgtcctt gaacgggtcg cccaccgtgt cgccgatcac cgtggccttg
      781 tgcgactcgg atcccttgcc gccgtagaga ccttcctcga cgagcttctt cgcattgtcc
      841 caggctccgc cggagttcga gagcatcacc gcgaggagct ggccggtcag gatgaccccc
      901 gccaggaacg cgcccagggc ctcggcctcg aaggcgaacc ccacgatgat cggcgtcagc
      961 accgccagca ggccgggggt gagcagctcg cgcagggacg tgcgcgtgca gatgtcgacg
     1021 acgcgcccat agtcgggctt ctcggtgccg tccatgatcc ccgggtggtc gcggaactgg
     1081 ttccgcacct cgaccacgac ctgggccgcg gcgcgcccga ccgcccggat tgcgagcgcc
     1141 gcgaacagga acgagaccgc gccgcccacc agaagcccga tcaggacctc gggctgatcc
     1201 acgcggatcc cgatgaagct gtagccggcc tccttcagag cctcctcgaa cgagccgaac
     1261 agcgaggtgg ccgcgatcac cgcggtggcg atcgccatcc ccttcgtgat cgccttggtg
     1321 gagttgccga cagcgtcgag cccctccagg acctcggccg gccgcccctc gaactcgccc
     1381 gacatctcgg cgatgccctg tgcgttgtcg gccacggggc cgaacgtgtc catcgacacg
     1441 atgaccccga cggtcgtcag catccccatg ccggtgagcg agatgaagta gaggcgctcg
     1501 gcgatgtcgt tgcccaggag gtacgccgag aagatcgtcc cggcgatcac gaggatcgcc
     1561 cacacggtgg actcgaggcc caccgagaaa ccggagagga tcgtggtggc ggcgccggtc
     1621 tgcgacgact ccgcgacctc cttcacgggg cgacgcttcg tggaggtgta gtgctcggtc
     1681 aggacgtgga tcacgctggc caggagcagc ccgatcacga cagcccagaa cacccggagg
     1741 tcgttcacgt aggcgtcggc cacgaagtac acgaccgccg cggacagcac cgccgagatg
     1801 aagaagcctc ggttgatggc ccgcatgccg tgctcggtgt cgctcctcgg cgtcaccgcg
     1861 tagatgccga cgatcgaggt gatgaccccg atcgcccgga cgaagagcgg gaacatcacg
     1921 ccgacgatcg cgccctcggg cgaatcggcg aacgccgcgg cacccaggat gatcgacgcc
     1981 accagcgtca cctcgtagga ctcgaagagg tcggcggcca tccccgcgca gtcgcccacg
     2041 ttgtccccga cgttgtcggc gatcgtggcc gcgttgcggg ggtcgtcctc ggggatgccc
     2101 tgctcgacct tccccacgag gtcggcgccg acgtcggccg ccttcgtgta gatcccgccg
     2161 cctacccgca tgaacatcgc gagcagggcg ccgccgaacc cgaagccgac gagcaccgag
     2221 gtcgcgtcct gcttgtagat caggaggatc acgacggccc ccagcagccc caggcccacg
     2281 gtgaacatcc cggccacacc tccggtgcgg aacgcgatga cgacggcgcg cttcaggccc
     2341 gtctccttcg cggcgttggc gacccgtacg ttgccgcgca ccgcgagcca catcccggcg
     2401 aagccggtga cggccgagaa gccggctccc aggatgaagg ccaccgatcg gcccagcctg
     2461 atcgcgatgg ccgaggtctc cgcgttctcc ggcacgggga ggaggaagaa gatcgcgatc
     2521 gtcagcacca cgaggaacat cccgaccgtg cggaactgcc ggttaaggta ggccctgctc
     2581 ccctgctgga tcgccgtcgc gatctcccgc atcttctcgg tgccctcagg tgctgcgagg
     2641 acgccgcgcc ggagcacgta cgccacgacg agcgcgagca ccgagatccc gaggatgatc
     2701 cacagcaccg cgtcctcgaa cgacccgaac tcggcagaga tcggaccgcc ttcctgggca
     2761 agcacaacca tggggtgttc ctccttggag tgagttgtgg ccggcgcgaa gagggcgctc
     2821 ggcgggccat agcctagcgg gtcgagactg acgcgaccaa caggcttgct cccgcgtcga
     2881 ggtctgctac cctgccgggc ggtttgcctc gaagagcaca cgccgcatcc ggtcgggccc
     2941 ttccctggat cctcgtcggc ctcctcgtcc tggtcgcctc gggcgccggg gcctgggccc
     3001 tgctccggga cggcgccccg gaggacgagg aggacctccc cgtcccggac ctcgtctgga
     3061 ccatcgggcg ggtgcgctcc acctcggtcg acgccgacgc ctcgcaggga gacctggagg
     3121 aggcgaggga cgcgatcgcc gacctcctcg acgacctgta cacgaccgcg ttcgtggacc
     3181 ccgcgaagtg ggaggggggc gcgttccccg agctcggctc gtacttcgcc gggagtgcgg
     3241 ccgcgcgcgc cgaacgggac cgggacggcc tcacgctggg tgaggacgcc gcccggatcg
     3301 agcgggtcga gccgacgagg gccaccctgc gactcacctt cctcgtcgat ccgcaggcgg
     3361 ctccctacgc ggcggtggcg acggccggct tccgggccga cggccggacg accgacggcg
     3421 gcgcggtagc gatcgtccac cagggccggt acatggtccg gcggatcgag ggggagtggc
     3481 ggatcatcgg atacgacgtc gaggggtccc tggagtcggt cgccgacccg gggcccggca
     3541 cgccgggagc gacgccgtga ggcgcggggc agcgctcctc gtcgcgctcg tcgtggggct
     3601 cggggcttgg gtcgcggtgg ccgccttcct ctcggagccc cgaccggcgg gagcccagac
     3661 gccggccgtg gagatctcgc gggcgcagga cgcgcgctac accccgtcgc tgggaggtaa
     3721 ccgtccgatc ttcatcctgg cgatcggaag cgacgcgagg cccgacgagc aggtggaccg
     3781 gcggctcgcg gactcgctcc acatcgtcgc cgtgaatccc cgccagggga gcgcctcgat
     3841 cctcggcttc ccccgggact cctacgtccc gatcccgggg ttcggcgagg cgaagatcaa
     3901 cgagtccctc tacaacggcg gccccgagct ggtcgtggag acggtggagg gcctcacggg
     3961 gatccagatg gactactggg tcctcacctc gttccgcggg ctgatcggcc tggtgaacgg
     4021 gatcgggggg ctggagatcg acgtccccta ccccatggac gacttctact cgggggcgca
     4081 gttcgaggcc ggggagcagg tgatcgacgg ctcccaggcc ctcgcgttcg ctcggaaccg
     4141 tcacgacacg cccgagggcg acttcagccg ctccgagaac cacggccggc tgctgctcgc
     4201 tgcgctcgag cagcttcggt cgcaggtccg tcgggacccc acggtcctct tccagtggat
     4261 cgccgtgggc ctgcggaacg tccagaccga tctctcgttc gacgaggtct tcgacctgat
     4321 gatgacggcc ctgagcatcg accccgagag cgtgacgaac gcggtcgtgc ccggggggac
     4381 ggggagcgcg ggcgcggcga gcgtcgtctt cctgtccggc gacgcccccg cgatcttcga
     4441 ggacctgggc gacgacggga tcctccagaa ccctccgccc ccgcccggcg gctagcggct
     4501 ccgggggtcc ggggcctcct cttcctcctc ctcctcctcg cgcccggaga ccctgagcga
     4561 cagccagatc gtcgccgtca gcacggccgc gatcacgacg agcgaggccc acacgggcac
     4621 gtggtagacg tcggccagca gcatcttcac ccccacgaac cccaagacca cgcccagccc
     4681 cacgttgagg tagcggaacc ggtcggccat gcccgccagg aggaagtaga gggcgcgcag
     4741 ccccatgatg gcgaacgcgt tcgaggacca gacgatgaac ggctcgcggc tcacggcgag
     4801 gatcgccggg accgagtcca cggcgaacag cacgtccgtg gcctcgacga ggatcagcac
     4861 gacgaggagt ggggtggcca tgagccgccc ctcctgccgg gtcagcatcc tctgcccgtc
     4921 gtagtagtgc gtggtgggga tcacgcggcg cacgagcttc agcacgggat tgcgctcggg
     4981 atggacctct gcgccggagt gccgggccag cctgaacgcc gtgtagacga ggaaggcgcc
     5041 gaagacgtag aggacccagt caaaccgctc cagcagcgcc acccccgcga agatgaagat
     5101 cgcccgcatg acgagcgccc cgaagacccc ccagaacagc acgcggaact ggtactcgcg
     5161 gggcacgtgg aagtaggtga gcagcaccgc ccagacgaac acgttgtcga tcgagaggct
     5221 cttctcgatc aggtagcccg cgaagtactc ccccgccgcc tgcccgccct gccaccagag
     5281 caggacgaac gtgaagctga tcccgatcgc cacccacacc gcgctctcga cggccgcctc
     5341 cttgaacgag atcacgtgcg gcgtccggtg gacgatcagg agatcgatca ggagcagggt
     5401 gacgatggcc ccgatgagga cggcccacac ccacatcggg acgtcgaagc tggcgaaccg
     5461 agcctgtgct tgctctgcgg cggctacgct cattgcgccc acgggcggag gatacccgaa
     5521 gggaaccgac atcacgcccg ggcggagcgt gagaacatcg gcgggcatgg acccacacga
     5581 cctcctcgcc accctccgga gcgaggcgcc cgaccggctc gtccacctgc ggaccctgcc
     5641 tgagcgccgc ccccggctgg cgcggctgcc cgatcttccc gagcccctcc ccgcgcggct
     5701 cgacaacctg gggatcgagg gactctaccc tcaccaggcc gaggccctcg agctgctcga
     5761 ggaaggacgg aacgtcgtcc tctcaacggg tacggccagc gggaagagcc tcgtctacaa
     5821 cgtgcacctg gcccggacgc tcctgcggga tccccgaagc accgggctgt acctcttccc
     5881 cacgaaggcg ctggcccggg accagctccg gcaggtgcgg ggcctgcggc tcccccagat
     5941 caaggcctcc gtctacgacg gcgacacgcc gagggcggag cgccccctga tccggaggac
     6001 ggccaacctc atcctctcca acccggacat gctgcacgcc tcgctcctcc ccgaccacgc
     6061 tcggtgggcg gactttctcc tgcgcctcgc cgtcgtggtg gtggacgagg cccatgtggc
     6121 ccgcggggtc ttcggctcgc acgtggcgat ggtcctgcgg cgcctccggc ggctggtggc
     6181 caagtacggc ggcgacccga ggttcgtcct ggcctcggcc acgatcggga atcccgcgga
     6241 cctggccgag cgtctcaccg gggtgccgtt cgcccaggtg accgaggacg cctcccccgc
     6301 gggcgagaag ctcttcgctc tgtggaaccc cccgctcgtg gacgacgagg agggcgggcg
     6361 gaagggggcc gtctccgagg cggccggcct gctggcgcgg ctcgtggaca gggacgagcg
     6421 aacgatcgcg ttcgcgcggt cacggcgcgc ggcggagctc ttggccgaga tcgctcgccg
     6481 cgaggtgggc gccgcgcgca agggcaggat caagacctac cgcgcgggct acctccccga
     6541 ggagcgccgt cgcctcgagc gggcgctgtc ggaaggggaa ctgctcggcg tggcctcgac
     6601 gaacgccctc gagctcggca tcgacatcgg ctcgctggac gccgccatcc tcgccggcta
     6661 tccggggacc cgggcctcga tgtggcagca ggccgggcgg gcgggccgcc gctccagcgc
     6721 ctcgatcgcc ttcctcgtgg cccaggacga cccgctcgat cagttcctcg tgtcccaccc
     6781 gcaggacctc ttcgacaaac cgcccgagga cgcggtcgtg gaccccacca acccgtacgt
     6841 gctggccccg cacctggcgt gcgcggcccg ggagcacccg ctcgcccccg aggagctctc
     6901 cttcttcggc gacgcggccc ccgacgcggt ggcctcgatg ctcgaggaag ggcgtctcgt
     6961 cgaacgcagg gggctcctct accaccgcgg gggcccgtcg ccccaccggg aggtggacat
     7021 ccgctcggcc tcgggttccc tctacctcat cgtcgaggtc gacacgggcg cggtgctggg
     7081 caacgtggac gagtcccgtg cgttcttcca ggtccacccc ggcgcgatct acctccacca
     7141 gggggagcag tacgaggtcg tcaccctcga tcgagagcgg aaggccgctc tcgtccgccc
     7201 cagtgacgcc gactggttca cccagtcgcg ggacgtcacc gacatcgagg tgcttcgggt
     7261 gcgcgccgag acgaaggtgg gctcgacgcc ggcgttcttc ggcgacgtcc gcgtcacgac
     7321 ccaggtggtg gggttccagc ggcgcctgca catcaccggc gaggtgctgg gcgtcgtgga
     7381 tctcgacctc cccccgcaga cgctcgtgac ccgcgccctg tggtgggccg ttcccgacga
     7441 gacgatcgag cgggcggggg tgacgccctc ggccctcccc ggggccgcgc acgccgccga
     7501 gcacgcggcc atcggcctcc tccccctcgt cgccacctgc gaccggtggg acatcggcgg
     7561 cgtctccacc gcccaccacc ccgacaccgg ctcgtgcgcg atcttcatct acgacgggta
     7621 cccgggcggg gccggcatcg ccgagcgcgg cttcgcctcc ggcgagcgcc tgctccgggc
     7681 cacgctggag acgatccggg cgtgcccgtg ccagagcggc tgcccctcgt gcgtccagtc
     7741 ccccaagtgc gggaacggca acgagccgct ggacaaggac gcggccgcgg cgctgatcga
     7801 ggagatgctg gcgtgatcca aatccagatc gccgtctgcg ggcccggcgt cgccaccgag
     7861 gaggaggggc gctgggccga ggaggccggc cgcctgctgg ccgaggaagg cgcggtggtg
     7921 gtctgcggcg ggatgacggg cgtcatggac gcggccgccc gaggcgctcg ccaggccggt
     7981 gggctcgccc tcgggatcct ccccggccgt ggcctcgagg gcgcgagcga gcacctctcc
     8041 gcggcggtcg tcacggggat gggcgaagct cggaacgcgc tcgtggtccg ctcggccgac
     8101 gcggtgctgg cgatcgccgg cgagtacggc acgctctccg agatcgcact ggctctgaag
     8161 atgggcgtgc cggtggtggg gctccgcacc tgggagctgg ccaagggcgg cgagccggtg
     8221 gaggccttcg tccgcgccgc cggcgttccc gacgccgtcc gggaggcgat gcggctcgcg
     8281 cgggagcggc ggtcgtgaag cgccgggtcg tgaacgccct ggccaagcac gtggccaacc
     8341 ccgtgatgcg gcggctcgcg gggcgcatcc ccggctgggc cctgctggag accaccggtc
     8401 gccgcagcgg ccgagcccat cagatccccg tgggcgacgg cctccgcggc gacgccttct
     8461 ggatcgtcgc ggagcacggt cgccacgccg actgggtccg caatgtcgag gcggacccca
     8521 gggtgaggat ccgcgtccga ggacgctgga ggaccggcac cgcgcacatc ctggaggacg
     8581 acgacccgag agagcggctc cgccggctcg gaggcgtcaa cccctgggtc atccgcgtcg
     8641 ctgggaccga gcccctgacc gtccgcgtcg acctcgaccc ggcccctccc cgcccgggat
     8701 ccgtcctgcg cgacggcctc gtggccggcc tcgtggccgg cgcggtggcg ggggcgccct
     8761 cgggcttcca cgccctccgc cacggctacc tcctcgacct cctgcgggcg cccggagcgc
     8821 tcgtcgcgcc ccgggccgga gccgaggccc agctcgccct cacggtcccg gtccatgcgg
     8881 tgatctcggc cggctgggga gtcgtcctgg cgagcgcgct cccccgccga gccaccgccc
     8941 tgtggggggc ggcgggaggc gtggtcatcg cgacgctcga catcgtggtg atcgggagcc
     9001 gaatcccggt catccggagg ctccctctct ggccccaggt cgccgaccac gtcgcgttcg
     9061 ggaccgtcgc gggcgggatc ctcgccgcgc tcagggaccc cagctagccg cggaccgccc
     9121 ccaccagcct cgccagcacc cgtcgctccg ccggaggcag gccgaacgcg accaacacgg
     9181 cgacatacgc cagcgtcccg atcccaccgg tgaccgccag gtccaccggc cacgaagccc
     9241 ccgctacgag ggcgtgggcg cccagggccg cgccgaggcc gagggccagc ggaacggcga
     9301 gcagagcgta gtgccgggtg aacggctgca tccccatgaa cctccacacc agccagaggc
     9361 gagccacctt cgagagggcc atcgtcgtgg ccccggcgat cgccgctccc tcgataccca
     9421 gccccagccc cccgatgagg aacgccgccg ccccgatgtc gaacgcgacc gacgccgcgt
     9481 agacgaggag gtcccatccc gtcctgccga tcatgatgag cacgaacccg accgcgccgg
     9541 tggcaacgtt gacgagctgg cccaagagga ggatccccag ggcggtggcc cccaccccga
     9601 actccgcccc gaagagacgg agggcggact cgggcgccgc ggtgagcacg atgaacaccg
     9661 ggagggtcgc ggccagggtc cagcgggtga gctgcttgta gagccgatcg agcttctccc
     9721 gctcgccccg ggcgtgaagg tccgcaacga aggggctgaa catgaggctg acggagatga
     9781 tgaacagcag caggacctgt cccgcccggg cggccgcgga gtagatccca acctctcgtg
     9841 aggaggcgaa gacggcgagg acgaagacgt cggtccagaa gagcagctgc gagaagaggg
     9901 cggccggagc cctcggggcc ccgtagcgca tcagatcccg gacctccccg gggaggggag
     9961 ggtcgccacc gaacccccgg gtctctcgat accagagcca agcggccgcg gccgtcgcgc
    10021 ccacccagga gacggtgtag gcgagggtcg tcacccccac ggacctggag acgagccagc
    10081 cggccaggat cagggcgaac cacgccaggg gctggcccat ccagaagacg tacagagtgt
    10141 ggcgcatgat cttcagcccg cgggtcgccc ccaggtacac ctggaccagc gcggtgaagg
    10201 ggatcgcgag ggccgccgcc cggaacgccc cggtggccgc gcccgccgcg tcggcggcca
    10261 gcgagcgcgc gatgggctcg gcgagcagga acacggcagc ggccacgatc aggctcgaga
    10321 ccgtcgagat gatcgatgcg cgcatgacca ccgcccgcac ccgcgagcgc tctccccgtc
    10381 ccacgtcgat ggccacgcgt cggacggccg ccatgtccat cccgaagcga gcgaagtagc
    10441 ccaggaccag cgcgccctgc gtcgccagcg tcacgatgcc gaaggccgag gcccccagcg
    10501 cccgcgtgat gagcacctgc gtgccgaagg tggccacgat cgcgaccacc atcccgacca
    10561 cgttctggcc cgtgccggcg aggatggtcc gctcgtcgcc gaagcgctcc agcgggagca
    10621 ccgactccag ggcgctgcgg atccttcggg ctcgcttccc gctattcgag gtagccaagg
    10681 ccccggaggt gctcctcgac ctcggccgcc tcgtcggcgt cgagccccgt gtcctcgtgc
    10741 tcctccgccg tctccgacga agccgcggcc tccgcggcgg tcgagcgctc cgccccaacc
    10801 accgactcca ggacctgccc gtcgtgcttg accgaagacg tcacgccgag cgcggcgagg
    10861 gtcgtcgggg ccacgtccac caggagggcc ggccgctcga aggcgtcggt ccgcacgtga
    10921 gggccggccg ccgcgatcac gccgtccatc cggtggtccc cggagagcca gtccgcgtcc
    10981 tcgatcgcgg tcttcgcgtg cgtgaggctg tagccctcgg ccggctccat gatgatgtcg
    11041 ggcgcggtgt cggcgaacct cccctggaag aactcctcgc gcttgtggat cgccttcacc
    11101 ggcttacgcc cggtggccgg gtcgacgaag cccgagagcg cgtccatcac cttctcgcgg
    11161 gtggactcga agtccgcggg atccacggtg ccgtcgggct cccgcccggc gaggttgatc
    11221 gagaccccct cgcccgtcga ccgcaccgac gtgtaggcga tgctcttccg ccagttaacc
    11281 gactgcggca gcgagacctt gccgtggagc ccgagcaggt cgtagacgcg ccgtgccaga
    11341 gcccgcaccc tcccccactg catcggcccg aagacggcgc tcgaggccgc aaacctcagg
    11401 aacccgaggt gctcgaggaa cttgtccatg tggagcgccc gggtgcacga ctggaacccg
    11461 tgatctgaga tgaacaggat caggtcgtcg tcgcccacat ggaccaggtg ccgccccagc
    11521 ccctcgtcga gcagccggta cacgtcccgg atcctctcgg ccagcggctc cttcgacagc
    11581 gtgtcgtagt tcggatggtc gggcgagacg tacttggcca ggcagtgctg gacgcgatcg
    11641 gtggccacgt acacgagcga ggcgaaccgc cagtccgtcg tctccagcag gtgctcgttc
    11701 gcatcgatcc gctgtctggt gagcgtcatc gcctcgtcca ggaacggctc gggccggttc
    11761 cagaacgtcg tccaggacat cccgttgatc gggaagggct tcccggcggc ctccagctcc
    11821 gcggccaggc cctcgggatg ggtgtgcggg cgacccttcg gcaggacccc gcccgagatg
    11881 agcttcccct ggatccgctg gggcgggaag gtcagcggga cgttcgtcat gatcgtctcc
    11941 acgccggccg cggcgagatc cgggaggatc gtccgctcct tgatcgatcg gtaggtcacg
    12001 ggcagctgct tccgcgtccg gaagtcccgc tcgaagatgt cgtagacgcc gtggttcgag
    12061 ggctcgagcc ccgtgaggaa cgagggccag gccgaccacg agtgattcgg gaccgtggac
    12121 ttcaggacgc ccctggcccc ccggtcgatc agcgccttga ggttcgggag gaggcccgcc
    12181 tccagcatcg ggtcgaggat gcgccagtcc gcgccgtccc agccgatcag cagcactctt
    12241 gccatgcgga tcccttcgga gtggtcggtg cccgtgaggg cgactcggta gtgtagttgg
    12301 cctcgcggcg ggctgaccag cggctcacgc gacctcgctc gagaccagct cggcccggac
    12361 gatcagccgc tcccgcgcgc tcccgggcgg gtggcaggtc gtcagggtga gcaccgacgc
    12421 gccgcggacg tcggccagca cactcacgtc gtcgggcgtc gtcacccacg acccggccac
    12481 ctggtacacg caccgcccca cgggagtgac cagcacgacc tcgtcccccg gtcggagcct
    12541 gtggaggtcg gcgaacggct tcgagtacgt cgtgcggtgc ccggccacca ccgcgttccc
    12601 cacctcgcac gggagcgcgc tcccttcgta gtggcccgcc ccggcgcgca gggccccggg
    12661 ggaggttccc tcgacgacga tcgacacgag ctccaggcga gggatctcga ccttcgtcag
    12721 cgcctctccc gggagcacgg cccgggccgc gtaggcctcc ctggtctcgg gctcggcgaa
    12781 cgacgcctcg agccccgatt ggcgccagtc cccgtagagg ttcgtgagat gcgggtagaa
    12841 gaccatcgtg aggcccccca ggaggaggaa gaccgagagg gtcccgagga cgcggcgtgg
    12901 gactggcctt cggcgccagg cgtcgaggac cagcccgccg acggaccgga ccccgctcac
    12961 ctcagggtcc gaggagagca cggtggaggg gtagagcggc tcctgctcct cccgggccat
    13021 gggccggagt gtatccccgc gggcggcgcg cgatgttgac acaccgctgg atcgtggtat
    13081 atatcgagac gatatatcga accgatacat cgaaccgata catcgaaggg agagaggtgc
    13141 tcgagttcgc catcctaggg cttctccagg agcggtccat gcacgggtac cagctgaagc
    13201 agcggctggc cgacgcgctg ggccccttct ggcaggtctc ctacggctcc ctctacccgg
    13261 cgctgaagcg gctgcagaag cagggcgccg tcgagatggt cttccccaag gccgaggtgg
    13321 gccgccgcaa gaacgtctac cggatcaccg agcacggcca ggagctcttc gccgagctcg
    13381 gagagagcac ccaggaggac tccgacgaca acggcttccg cgtcaggctg gccttcttcc
    13441 gccacctgaa gccggaggcg cggatcggcg tgatggagcg ccggcgcgcc gtgctggtcg
    13501 atcgactccg cgagctgaag gaccggctgg cctcctaccg cgagcgcgtg gacggctaca
    13561 cgctgtcgct gatgcgccac ggcatggaca cgaccgaaca cgacatccgc tggctcgacg
    13621 acatgattca ggcggagcgc agggccagcc ggagcggccg caagcgccgc gctccgaaga
    13681 agggagtcag ggcatgagca aggtccgact ggccatcgtg ggggtgggca actgcgcctc
    13741 ctccctcgtg cagggactgg agttctacaa ggacgccgac cccgccgagc aggtccccgg
    13801 gctgatgcac gtcctgttcg gtccctacca cgtctccgac gtggaggtcg tcgcggcgtt
    13861 cgacgtggac ggcaagaagg tggggcgcga cgtctccgag gcgatcttcg cggagcccaa
    13921 caacacgatc cggttctccg acgtcccgcc cacgggcgtg gaggtcctcc gcggcccgac
    13981 cctcgacggc ttcggccgct actaccgcga ggtcgccgag gagtcggacc tcgagccggt
    14041 cgacgtggcc gaggcgctcc gcgacgccgg gaccgacgtc gtgatcagct acctccccgt
    14101 ggggagcgag caggccgcgc ggcactacgc gcaggccgcg atcgacgcgg gcgccggctt
    14161 cgtgaactgc atgccggtgt tcatcgcctc cgaccgggcg tgggcgaagc gcttcggcga
    14221 cgcccgcctc cccatcgtcg gagacgacat caagtcccag gtcggggcca cgatcgtcca
    14281 ccgcatgctc gccaagctct tcgaggaccg gggcgtggtg ctcgaccaca catcccagct
    14341 caacttcggc gggaacatgg acttcatgaa catgctggag cgcgagcggc tgacctcgaa
    14401 gaagatctcc aagacccagg ccgtcacctc gcagctgcgg gacggcatcc cggccgagga
    14461 catccacatc ggcccctccg accacgtgcc gtggctgcac gaccggaagt gggcgcacat
    14521 ccggctcgag ggtcgggagt tcggcggggt cccgctctcg gtggagctga agctcgaggt
    14581 ctgggattcg ccgaactcgg ccggcgtggc gatcgatgcg atccggtccg cgaagctggc
    14641 caaggaccgc ggcgtgggtg gcccgctggt cggcccctcc gcctacctga tgaagtcccc
    14701 gccggttcag ttctccgacg acgcggcccg cgacatggtc gaggagttca tcctcggcaa
    14761 cggcggcgcc aaggatcagg actggacgaa gttcttcgac gtccagcctc aagagagctg
    14821 agcgggagac cctcgggagc ccctcccgag gatcgtcccc gcctacactt cggggatgag
    14881 cggacgtccg cggctgcccg gcctccgcga cctctcggga cggcgtggct tccggcgact
    14941 gatcgcctcg caggtcacct cccaggccgc cgacggcctg taccagatcg ccatcgcagc
    15001 cgtcttgatc ttcggcgtgg acgcggcgga gaccccgggg caggtcacga aggtcctcgc
    15061 cgtgacgctc ctgcccttct cgctggtcgg ccccttcacg ggcccgttca tcgatcgctt
    15121 ctcccgacgc tcgatcctcg tggggacgag cgtggctcgg gccgcgctga ccttcgccct
    15181 gatccctgcc acgggctggc cggagtggat catcctcgcc ctggtggtcg ccaacatgtc
    15241 cgtgaaccgc ttcttccacg ccacgaagag ctccgccctc cccacgctcg tcccgcggca
    15301 ccggtacctg ctggcgaaca cggtttcctc ggtgggaggg atggtgttcg gactggcggg
    15361 cgcggtggtg ggggggcccg tcgccgacgc cgtctccgcc gaggtggtga tcctggccgc
    15421 cggggtgctg atgaccgtcg cggccgtcct cgcgacgacg gtccccctcc cgccgggaga
    15481 gcggaagggg ctgacggggc tgctggggga gctccgggac gacgcccggg acgtccgtga
    15541 cgggctgcgt ctgctcctgg gcaccccgcg ggccgtctac gccatctcct ccatctggtc
    15601 catccggggg ctgatcgggt tcctcctgct ggccgggatc gtcctgctcc ggaggaggtt
    15661 cggcctggag acgacgggcg cctccctcct gctgggggcg gtcggcatcg gtgggttcct
    15721 gggggcgctc ctggtgacgc ccgcggcacg ccggctcggc gacgcccggg tcgcaccctt
    15781 ggcgtacctg gttgccggga tcgccgtcct ggcgctcggg gccgttcccg cgtggcccgc
    15841 gctcgtgacg ctcgtggccg tggggggcgc cgcgatggcg atgacgaaga tcaccgccga
    15901 cacgatggtc cagcgctcga tccccgactc ccatcgggga cgggccttcg ccatctacga
    15961 catcggctac aacggcgtct tcgtcctcgc cgccctgatc cccaccctcc tcatcgggct
    16021 actgggcgac atcgggatcg tcttcctggc cgggaccctc gcggtgatcg gagcggggat
    16081 cctcgccctc gcctcgcgaa ggctcggtgg gctcgaagtg cgcgcctacg ctgggggacg
    16141 ggccgacgag actccgcgcg aggtcgtgat cgacggaacg aggctcatgg tgacgggggt
    16201 ggagcgctcc tggcacgagg agcgggcggg ccggcgcctc ctcgtcttcg tgctgcgcct
    16261 ggaggacggc aggcgcctcc agctgagccg aggcgaggag tggcggatcg accgcttcct
    16321 gggcgagccc ggctagagga gccgaggatc cggcaggtac cggagcacgg ccacacccag
    16381 gatgtctcgg cgagccaccg ggccgaacga ccgcccgtcg gtggatcgca gggggtcgtc
    16441 cccttcgacc tggtactcat cctccccgag cgtccgcgcg cccatcgggt cgccgggaag
    16501 tcccgtgacc cgcttcacca tctccacgtc gtggctcggc gggcgcacca cgaccacgtc
    16561 gcccctccgg atcctccgcc gtcgcacgca aaggagctgg tcgcccggac gcagcgcgga
    16621 ggccatgctc acgccctcga cgaccacccg gaacggcctc cgccaggcca gccacccggc
    16681 gaccgcggcg agcaccggca gcgcccctcc cctccgcatg cgcgaagcct acccgggtag
    16741 gaagtgtgtg gccgctcgca acgagcgggt ggtagcgtga gagcccacca gaggaaggag
    16801 gaggcatgct cagccgactg ctgaggccca ggcgggtcgt ccgcgcgcac tgcgacctcc
    16861 cctgcggcgt ctacgacccg gcccaggcca ggatcgaggc ggaatcggtc cagaagatcc
    16921 aggagaagt
//
LOCUS       DASUTW010000038         2546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1437982, whole genome shotgun sequence.
ACCESSION   DASUTW010000038 DASUTW010000000
VERSION     DASUTW010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437982"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..686)
                     /locus_tag="VF058_01370"
     CDS             complement(159..686)
                     /locus_tag="VF058_01370"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017540365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX6206985.1"
                     /translation="MNNNLERLRELIRDVPDFPQEGVMFKDITPVLADPAAFSTAIDM
                     IVVHFGRGNVDRVVGIEARGFILASPVAYHFGAGFVPVRKKGKLPWRTRDQAYELEYG
                     TEVLEIHEDALRPGERVLIVDDVLATGGTARATAELVESLGGKVVGIAVLLELSFLEG
                     RKQIEGYEVYSLLTS"
     gene            complement(727..1911)
                     /locus_tag="VF058_01375"
     CDS             complement(727..1911)
                     /locus_tag="VF058_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006139931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX6206986.1"
                     /translation="MSTPVLLIELGVVILGLAVLARFAERAGFSPIPLYLLVGLAFGD
                     GGILPVVTSEEFIEVGAEIGVILLLLMLGLEYAGDELTESLRKNAAAGALDLALNFTP
                     GFAAGLLLGWGPLAAVILGGITYVSSSGIVARVVQELGWVANAETPTVLSILVIEDLA
                     MAGYLPLIGVLLRGEDPASAGLAILAAVGAVLVTLFVAVRFGPFLSRLAFSRSDEGLL
                     LTIVGIALVVAGLAERLQVSAAVGAFLVGIALSGHAADRARVLLTPLRDLFAAVFFIF
                     FSLQIDPSEIPSVLGVAAALALVTGASKVATGWVSAGIRGLRSGGRLRAGTALIARGE
                     FSVVIAGLAVGAGVEPSLGPLTATYVLILATIGPFAAQAAGALSTRGAGRRESRPEEG
                     EA"
     gene            complement(1915..2397)
                     /locus_tag="VF058_01380"
     CDS             complement(1915..2397)
                     /locus_tag="VF058_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017869955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter regulatory subunit"
                     /protein_id="HEX6206987.1"
                     /translation="MAEVEETKLPGLGIRFEFLTSRGSRIGVVHHRTGRRELFVHDPD
                     DETSGTITALDEDDSRTLAELLGGSRVGEQLANLQQRVEGIAIDWLPIADGTPFAGKT
                     IGDTRARTRTGTSIVAIVRGEDAVPAPGPEHPIEAGDTLVVVGTPRGIEQLAVSLRTG
                     "
     gene            complement(2414..>2546)
                     /locus_tag="VF058_01385"
     CDS             complement(2414..>2546)
                     /locus_tag="VF058_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007933982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEX6206988.1"
                     /translation="IDTSISHVMEYFEIFLDRMAMCRRAAEELECRFRVVINETEVL"
BASE COUNT          417 a          959 c          837 g          333 t
ORIGIN      
        1 agcccgtcga acccctcggc tcgatcggga tggctcgaca cgaagggaag gcgcgccagg
       61 aggccgctcc gtcgctccgg tgcgcgaacg tcctccacga gtgcacccct ctcctgacct
      121 cctctattga agcacgggcc caggcggggc gtcccggctc aggacgtcag cagggagtag
      181 acctcgtatc cctcgatctg cttccgcccc tcgaggaagg acagctcgag gagcaccgcg
      241 atgcccacga ccttgccccc cagcgactcc acgagctcgg cggtggccct cgccgtgccc
      301 ccagtggcca ggacgtcgtc cacgatcagc acgcgctctc ccggccggag ggcgtcctca
      361 tggatctcca ggacctcggt gccgtactcc agctcgtagg cctggtcgcg ggtcctccag
      421 ggcagcttcc ccttcttgcg gacgggcacg aagcccgccc cgaagtggta ggcgaccggc
      481 gaggccagga tgaagccccg ggcttcgatg cccacgacgc ggtcgacgtt cccccgcccg
      541 aagtgcacga cgatcatgtc gatcgcggtg gagaaggcgg ccggatccgc gaggaccggg
      601 gtgatgtcct tgaacatcac cccctcctgg gggaagtcgg ggacgtccct gatgagctcg
      661 cgcagacgct ccaggttgtt gttcatgcgc cctcattctc ccatccgagg gaggcggccg
      721 caccggtcac gcctccccct cctccgggcg ggactcccgc ctccccgcgc cccgcgtgga
      781 gagcgcgcct gccgcctgcg cggcgaaggg gccgatcgtg gccaggatca gcacgtacgt
      841 cgccgtcagc ggtcccagcg acggctccac gccggcgccc acggccaggc ccgcgatgac
      901 caccgagaac tcgcctcggg cgatcaaggc cgtcccggct cgcagacggc cacccgacct
      961 cagcccccgt atgccggcgc tcacccagcc ggtggccacc ttggaggccc ccgtgacgag
     1021 ggccagggcg gccgccactc cgagcaccga ggggatctcc gacggatcga tctgcaggct
     1081 gaagaagatg aagaagacgg ccgcgaagag gtcgcggagg ggcgtcagca gcacccgcgc
     1141 ccggtcggcc gcgtggccgg agagcgcgat gccgaccagg aacgccccca cggccgccga
     1201 gacctggagc cgctccgcca gcccggccac cacaagagcg atccccacga tcgtcaggag
     1261 caggccctcg tccgagcgac tgaacgcgag cctgctgagg aacgggccga accggacggc
     1321 cacgaagagc gtcaccagga cggcccccac ggccgcgagg atggcgagac cggccgaggc
     1381 cggatcctcc cctcggagga ggacgccgat cagcgggagg tagcccgcca tggccaagtc
     1441 ctcgatcacc aggatggaga gcacggtggg cgtctcggcg ttggccaccc acccaagctc
     1501 ctgcaccacc ctggccacga tccccgagga cgagacgtag gtgatcccac cgaggatcac
     1561 ggccgcaagc ggcccccatc ccaggaggag gcctgccgcg aacccgggcg tgaagttcag
     1621 ggcgaggtcg agggcgcccg cggcggcgtt cttccggagg ctctccgtga gctcgtcgcc
     1681 cgcgtactcc aggccaagca taaggagcag gaggatcacc ccgatctcgg cgcccacctc
     1741 gatgaactcc tcggaggtca ccaccggcaa gatcccgccg tcgccgaacg ccaggccgac
     1801 gaggagatag agggggatcg gcgagaaccc cgcccgctcg gcgaagcggg ccaggacggc
     1861 gagccccagg atcaccaccc ccagctcgat caggagcacg ggggtggaca cggctcagcc
     1921 ggtccgaagg ctcacggcga gctgctcgat ccctcgaggc gtccccacga cgaccagggt
     1981 gtcgcccgct tcgatcggat gctcggggcc cggagcgggg acggcgtcct cgccgcggac
     2041 gatcgccacg atggaggtgc cggtccgcgt cctggccctc gtgtcgccga tcgtcttccc
     2101 cgcgaagggc gtcccgtcgg cgatgggcag ccagtcgatc gcgatcccct ccacccgctg
     2161 ctgcaggttc gccagctgct cccccacccg cgacccaccg agcagctcgg ccagcgtgcg
     2221 gctgtcgtcc tcgtccaggg cggtgatcgt ccctgaggtc tcgtcgtccg ggtcgtggac
     2281 gaagagctcg cgccggcccg tccgatggtg gacgacgccg atcctgctcc ccctggacgt
     2341 caggaactcg aaccggatcc cgagacccgg cagcttcgtc tcctcgactt ccgccatggc
     2401 tccgacctcc cgatcacagc acctcggtct cgttgatcac gacgcggaag cggcactcga
     2461 gctcctccgc cgccctcctg cacatcgcca tccggtcgag gaagatctcg aagtactcca
     2521 tcacgtggct gatcgacgtg tcgatc
//
LOCUS       DASUTW010000039         3330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1179198, whole genome shotgun sequence.
ACCESSION   DASUTW010000039 DASUTW010000000
VERSION     DASUTW010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179198"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VF058_01390"
     CDS             complement(<1..531)
                     /locus_tag="VF058_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206989.1"
                     /translation="MVLLVSWMIGFSDRWVAVWSISWPALIFWMGIVLAIDFFPIKVG
                     SLHLTLDVPVLLAIALLYSPEVAASVACLAALDLREVKGGVGLGRAIYNRAQIALSVL
                     VAGHVLQLFGPDMGRTQSLILPTLAALLVDYAVNATLVVAVRVADGSAASFLAELWRL
                     KVGSWWQFFGTYLAYGL"
     gene            complement(697..>3330)
                     /locus_tag="VF058_01395"
     CDS             complement(697..>3330)
                     /locus_tag="VF058_01395"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX6206990.1"
                     /translation="RTIGRQRAELARMVADFQAAKREMGVVDYGDQIAHAAQLAKEHP
                     EVGERFRKRYSVVLLDEYQDTNLAQADLMRHLVGPGFPIFAVGDPDQNIYAWRGATLR
                     NILGFGDDFGASNGELPLYVNFRSGSRILAAANHVIEPVPVERRAAGKILHPHEPLGE
                     GRVLGFVAEDQRAEAGQIARMIEEMRGSPRRDGGALQYGDFVVLCRKKRLFGPIAEVL
                     RQEGLPVEIVDLGGLLQLPEIVDVVAWLRLLENPGDNVSLARILFGPRWRIGYRDLAV
                     LAHWSADQNRSLADELDADRSPGEVRFALVEAVDHLDEIEGLSPEARERLEEFRIDLA
                     GLRERVRQPLGDLVAEVLEVSGLLRELEASTSPPARAARRNLLNLVDHVAAFTSLDGE
                     ATLSAFIAYLNAALEAEDELEPAQASESDTVKLMTVHKAKGLEWPVVFVPGMAEHERP
                     ASSIFPNLRTRENPLLGKTAIPLEMRRDEADFPDFEGDIDAWGGALREQQLEEERRLG
                     YVALTRAQETLVVSAAYWYEGPQDPYRPSRFFDEVTGSPGAEVLFVAEQPEENPIAAL
                     LAEKAADWPGPGRREDADELFPEGWRGAAIEAASSPDAAERRAESVLGPRDLEDFRRL
                     GASHRERAARIEERVTSEGPPALPATLSVSSVIDLERCPKLFYWSHVRPLPRRPSSAA
                     RLGSEVHRWIELQSRGQASLLDVDEIPDLGTEERMGEPGVVAEMRRAFRESRFSESVP
                     LYAERPFLLYLEGSVVGGRIDAIFGEPHGPWEVVDYKTGRKPEDDPLAGLQLDLYALA
                     CVEIWGKAPEELTLTYFYLASGEEETRPAGDPEVTRKRVLEAIRRAASGEFEARPGPQ
                     CRWCDFRQFCEPGASWVREQG"
BASE COUNT          561 a         1151 c         1127 g          491 t
ORIGIN      
        1 cagtccgtat gcgagatacg tgccgaagaa ctgccaccac gacccgacct tcagtctcca
       61 cagttcagcg agaaaggaag ctgctgaacc atccgcaacc ctaacggcga caaccaatgt
      121 ggcgttgact gcgtagtcaa cgagcaatgc tgcaagcgtc ggaaggatca gagactgggt
      181 tcgtcccatg tccggcccga aaagctggag cacatgacct gctacaagaa cacttaaagc
      241 gatctgagcc cggttgtaga ttgctcgacc cagaccaacc ccgcccttca cctcgcgcaa
      301 gtcgagagca gcaaggcacg caacagatgc agcaacctca ggagaataca gcaaggctat
      361 cgctagcagc actgggacat cgagcgtcag gtgaagcgac cctaccttta tgggaaagaa
      421 gtcgatggcc agcacgatgc ccatccagaa gatgagggcc ggccaactga tcgaccacac
      481 tgctacccac cgatcgctga aaccgatcat ccatgagacg agcaggacca atagcccgag
      541 ggtcagaatg accgaccctg gcgattcgtg gctggtcctg gttcgaggaa gagtggtcga
      601 cctggttgcc atcgcagggg aatcgttgcg ccggggagga gcgagagtca atttctcgca
      661 gggtgaaatc ttgtcccctg gatgggggat ggagtatcac ccctgttctc ggacccagct
      721 ggctcccggt tcgcagaact gtcggaagtc gcaccacctg cactgggggc ccggcctggc
      781 ctcgaactcg cccgatgcgg cccgtcggat cgcctcgagg actcgcttcc gcgtcacctc
      841 cggatcgccg gccgggcgcg tctcctcctc tcctgaggcc aggtagaagt aggtcagggt
      901 gagctcctcg ggagccttcc cccagatctc gacgcaggcc agcgcgtaca ggtcgagctg
      961 gagtccagcc aacggatcgt cctcgggctt tctgccggtc ttgtagtcaa ccacctccca
     1021 gggcccgtgg ggctccccga agatggcgtc gatgcggccg cccacgacgc tcccctccag
     1081 gtacaggagg aacggacgct ccgcatagag gggaacgctc tcggagaacc tgctctcgcg
     1141 gaaggcgcgc cgcatctcgg ccacgacgcc cggctccccc atccgctcct ccgtgcccag
     1201 gtccgggatc tcgtccacgt cgaggagcga agcctgcccc cgggactgga gctcgatcca
     1261 ccggtgcacc tcgctcccga gccgcgcggc gctgctcggt cgccggggca gtggccgcac
     1321 gtgggaccag tagaagagct tggggcaccg ctcgagatcg atcaccgacg agaccgagag
     1381 cgtcgccggg agcgccggcg gcccctcgga ggtcacgcgc tcctcgatcc gggccgctcg
     1441 ctcgcgatgc gaggcgccga ggcggcggaa gtcctccagg tcgcgaggcc ccaggacgct
     1501 ctccgcacgg cgctcggcgg cgtccgggga ggaggccgcc tcgatcgcgg cgccgcgcca
     1561 cccttcgggg aagagctcgt ccgcatcctc gcgacgcccc gggcccggcc aatcggccgc
     1621 cttctcggcc agcagcgcgg cgatcggatt ctcctccggt tgttcggcaa cgaacaggac
     1681 ctctgcccca ggggacccgg tcacctcgtc gaagaagcgg gagggccggt acggatcctg
     1741 gggcccctcg taccagtacg cggccgagac cacgagcgtc tcctgggccc tcgtgagcgc
     1801 gacgtagccc aggcggcgct cctcctcgag ctgctgctcg cgaagggcgc ccccccaggc
     1861 gtcgatgtcc ccctcgaagt cggggaagtc ggcctcgtcg cgccgcatct cgagcgggat
     1921 cgcggtcttt cccagcaggg ggttctcgcg cgtccgcagg ttggggaaga tgctcgacgc
     1981 cgggcgctcg tgctcggcca tccccgggac gaacacgacc ggccactcga gccccttggc
     2041 cttgtggacc gtcatcagct tcacggtgtc ggactcggag gcctgggcgg gctcgagctc
     2101 gtcctcggcc tcgagggcgg cgttcaggta ggcgatgaaa gcggagaggg tggcctcgcc
     2161 gtcgagcgag gtgaacgctg cgacgtggtc cacgaggttg aggagattgc ggcgagcggc
     2221 gcgggccggc ggcgacgtcg aggcctcgag ctcgcggagg agtccggata cctcgagcac
     2281 ctcggccacg aggtcgccca ggggctgacg cacccgctcc cggagcccgg cgaggtcgat
     2341 ccggaactcc tccagccgct cccgggcttc gggcgagagg ccctcgatct cgtcgaggtg
     2401 atccacggcc tccacgagcg cgaaccggac ctcgccgggg ctccggtcgg cgtcgagctc
     2461 gtcggccagg gagcgattct ggtcggccga ccagtgggca agcacggcga ggtcccggta
     2521 gccgatccgc caccgcgggc cgaagaggat gcgcgccagc gagacgttgt cgccggggtt
     2581 ctccaacagc cgcagccacg ccacgacgtc cacgatctcg gggagctgga gcaggccgcc
     2641 gaggtccacg atctccacgg ggagcccctc ctggcgaagg acctccgcga tcgggccgaa
     2701 gaggcgcttc ttccggcaga ggaccacgaa gtcgccgtac tggagcgcgc cgccgtcgcg
     2761 tcggggcgag ccccgcatct cctcgatcat gcgagcgatc tgtccggcct cggcccgctg
     2821 atcctcggcc acgaagccca ggacgcgccc ctcccccagg ggctcgtggg gatgcaggat
     2881 cttccccgcg gcccggcgct ccacggggac cggctcgatc acgtggttcg cggcggcgag
     2941 gatccgagaa ccggagcgga agttcacgta cagggggagc tcgccgttcg aggcgccgaa
     3001 gtcgtcgccg aagccgagga tgttgcggag ggtggcgccc cgccaggcgt agatgttctg
     3061 gtcggggtcg cccaccgcga agatcgggaa gccggggccg acgaggtgcc gcatcaggtc
     3121 ggcctgggcg aggttcgtgt cctgatactc gtcgaggagg acgaccgagt agcgcttgcg
     3181 gaaccgctcc cccacctcgg gatgctcctt ggccagctgc gcggcgtgcg cgatctggtc
     3241 gccgtagtcc accacaccca tctcccgctt cgccgcctgg aagtcggcca ccatccgagc
     3301 aagctcggcg cgctgacgcc cgatcgtgcg
//
LOCUS       DASUTW010000040         4264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1049563, whole genome shotgun sequence.
ACCESSION   DASUTW010000040 DASUTW010000000
VERSION     DASUTW010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049563"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VF058_01400"
     CDS             <1..451
                     /locus_tag="VF058_01400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX6206991.1"
                     /translation="GALAPGYAFLFDYGFTGGERPGDPQAYRRHRVSGDVLADPGSRD
                     ITAAVDLDAVAARAREAGLQVWGPVTQREALLGVGMRLWLQGVRRRTQQAEERGDLDE
                     ARRLFEARSRASILIDETKLGGLRLLTLGSEGLPAPAAALGDPQAGC"
     gene            complement(617..1819)
                     /locus_tag="VF058_01405"
     CDS             complement(617..1819)
                     /locus_tag="VF058_01405"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX6206992.1"
                     /translation="MADTTDEQQDVYTAMGEQWLLRGGGVDLIRAEGQDLSTEEMVLN
                     IGPQHPSTHGVLRIVLDLDGERIRRAEPVIGYMHRAAEKLAEHRDWRQVLVLMNRHDW
                     LSAFNNELGWTIAVERMLGVEVPERAQWIRTMMAEWNRVLNHLMFMGSYPLELGAMTP
                     MFYAFREREMIQDLMESATGARMHHSYARVGGLKDDLPRGFLQRSGEVLTWIRAKLRD
                     FDNLIMGNEIIHNRTRGIGVLPADVAVSYGVSGPVLQASGVPMDSRKDEPYEKYGEVE
                     FEVPVGRNGDCYDRLWVLVQRMHQSCNIIEQCMEKLPAGEYRSASLPKKINIPEGEIY
                     VRTENPLGLMGYYVVGDGGEYPYRLKMRTASFSNVSVLPWLLRDQLLPDLIAILGSVF
                     FVVGDVDR"
     gene            1950..2702
                     /locus_tag="VF058_01410"
     CDS             1950..2702
                     /locus_tag="VF058_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007071686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6206993.1"
                     /translation="MSIGQDVTVNGTAARAVEAVKVYGSGEAEVRALDGITAGFARGT
                     FTAIMGPSGSGKSTLLHCLAGLDTLTTGRAFIGDADLTTLPERELTRIRRDKVGFIFQ
                     AYNLVPTLSAGENITLPLDIASRKHDEDWLASVVETMRLGDRLHHRPSELSGGQQQRV
                     AAARALVARPDIVFADEPTGNLDSKSGVELLAFLEGVVRDFGQTIVMVTHDPKAAAHA
                     DRVVFLADGRVADEMQGPTAESVLDRMKSLET"
     gene            2706..>4264
                     /locus_tag="VF058_01415"
     CDS             2706..>4264
                     /locus_tag="VF058_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6206994.1"
                     /translation="MLRATLKGLLAHKFRLALTAIAVVLGVAFVAGTLVLTDTIAKAF
                     DEVFASAQEGVDVEVRAEASDLAAGGDRERIPEELLSVIQRVEGVDRVEGGVTGYAQI
                     VDASGEATGTTGAPTFGISWPTYEGFSALSIREGRAPLTGGEVAIDAATAERHGFVIG
                     DRVTVLFQGPQEEFQVVGIAGFGDADNLAGATMAAFDVATAQRVLGAEDTFDSIFVAA
                     DEGVGAAELRDRISEVLPQGAEAQTGAAVAAEQADAIKDALGFFNTFLLVFAAIALFV
                     GAFIIFNTFNILVTQRTRELALLRALGASPRQVTLSVLTEAGIVGLAASVVGLGLGIL
                     VAFGLRALLAGFGIDLPSASLQILPRTIAVAFLVGILVTLVAALMPARRAARISPIAA
                     MKAVETVRPLDSRRRMVIGGALVAGGVALGLVGLFGGGDSALQAVGIGVAATFIGTAI
                     LSPLIAQPLARAIGSPLPRLAHLPGKLGRENAMRNPRRTASTSAALMIGLALVGTFLI
                     LGSSLKTSVGQT"
BASE COUNT          617 a         1497 c         1519 g          631 t
ORIGIN      
        1 cggggcgctc gcccccgggt acgcgttcct cttcgactac gggttcaccg ggggcgagcg
       61 ccccggcgac ccccaggcct accgccgcca ccgggtgagc ggggacgtgc tggccgaccc
      121 ggggagcagg gacatcacgg ccgcggtcga cctcgacgcg gtcgccgccc gggcccgtga
      181 ggccgggctc caggtgtggg ggcccgtcac ccagcgcgag gcgctcctcg gcgtcgggat
      241 gcgcctctgg ctccagggcg tgcggcgccg gacccagcag gccgaggagc ggggcgatct
      301 cgacgaggcg cgacggctgt tcgaggcccg cagccgcgcc tcgatactga tcgacgagac
      361 gaagctgggg ggcctgcgcc tgctgacgct tggctccgag gggctgccgg cgccggccgc
      421 ggccctgggc gaccctcagg ccggctgctg aggaccgacg acccgggcga tccagggcag
      481 cttgagcaac gcgtcccgtc tgctctgcgg aagcgagtcc gccatggcct cgagatcggt
      541 gccgcagcgc cgcagaatcg gaactcggac gggacgacgc cgcagcgcgg gcaggtcctc
      601 tgcccgggag cggccactac cggtccacgt cgcctaccac gaagaagacg gagcccagga
      661 tcgcgatgag atcgggcagc agctgatccc tgagcagcca cggaaggaca gagacgttgg
      721 agaacgaggc cgtgcgcatc ttcaggcggt aggggtactc accgccgtcg ccgacgacgt
      781 agtagcccat cagcccgagc gggttctcgg tgcgcacgta gatctcgccc tcggggatgt
      841 tgatcttctt ggggaggctg gcggatcggt actcgcccgc gggaagcttc tccatgcact
      901 gctcgatgat gttgcacgac tggtgcatcc gctggacgag cacccacagg cggtcgtagc
      961 agtcgccgtt ccgccccacc ggcacctcga actcgacctc gccgtacttc tcgtagggct
     1021 cgtccttgcg cgagtccatc gggacgcccg aggcctgcag gacgggcccg ctgacgccgt
     1081 aggagacggc cacgtccgcc gggaggaccc cgatccccct cgtgcggttg tggatgatct
     1141 cgttccccat gatgaggttg tcgaagtcgc ggagcttggc ccggatccag gtcagcacct
     1201 cgccggaccg ctggaggaac ccgcggggga ggtcgtcctt caggcccccc acccgggcgt
     1261 aggagtggtg catccgtgcc ccggtggccg actccatcag gtcctggatc atctcccgct
     1321 cgcggaacgc gtagaacatc ggggtcatcg cgccgagctc cagggggtac gagcccatga
     1381 acatcaggtg gttcaggacc cggttccact cggccatcat cgtgcggatc cactgggcgc
     1441 gctcggggac ctcgacgccc agcatccgct ccacggcgat cgtccacccc agctcgttgt
     1501 tgaaggccga gagccagtcg tggcggttca tgagcacgag cacctggcgc cagtcgcggt
     1561 gctcggcgag cttctcggcc gcgcggtgca tgtagccgat caccggctcg gcgcggcgga
     1621 tccgctcgcc gtcgaggtcg agcacgatac gcaggacgcc gtgggtggag ggatgctggg
     1681 gcccgatgtt gaggaccatc tcctcggtgg agaggtcctg cccctcggcc cggatcagat
     1741 ccacgccccc accgcggagg agccactgct cccccatcgc cgtgtagacg tcctgctgct
     1801 cgtcggtcgt gtcggccatc gacgcgccta gggtactcga acgggccgcc caacccgcaa
     1861 cccttcaccc cgcgagcgga accgtctatc cttggcgccc gcgccgacgg gcgaccggcc
     1921 cctaaacgtg ccacgaggag gagtgttcga tgagcatcgg gcaagacgtg accgtgaacg
     1981 gcaccgccgc tcgcgccgtg gaggccgtca aggtctacgg gtcgggagag gcggaggtcc
     2041 gcgccctcga cggcatcaca gcggggttcg ctcggggcac cttcaccgcg atcatgggcc
     2101 cctcgggctc cggaaagtcc accctgctcc actgcctcgc gggtctggac acgctgacga
     2161 cggggcgcgc cttcatcggc gacgccgacc tgaccaccct ccccgagcgg gagctcacgc
     2221 ggatccgccg cgacaaggtc ggcttcatct tccaggccta caacctggtc cctacgctga
     2281 gtgccgggga gaacatcacc ctgccgctgg acatcgcctc gaggaagcac gacgaagatt
     2341 ggctcgcgag cgtcgttgag acgatgaggc tgggggatcg cctgcaccac cgcccctcgg
     2401 agctctccgg cggccagcag cagcgagtgg ccgcggcccg cgcgctcgtg gccaggcccg
     2461 acatcgtctt cgccgacgag cccaccggga acctcgactc caagtcgggg gttgagctgc
     2521 tggccttcct ggagggcgtg gtccgggact tcggccagac gatcgtgatg gtcacgcacg
     2581 acccaaaggc ggcggcccac gccgaccggg tggtcttcct ggcggacgga cgggtcgccg
     2641 acgagatgca gggccccacg gccgagtcgg tgctcgaccg catgaagtcg ctcgagacct
     2701 gaaccatgct gcgggcgacc ctcaaggggc tcctggccca caagttcagg ctcgcgctga
     2761 ccgcgatcgc cgtcgtgctg ggggtcgcgt tcgtcgccgg gaccctggtg ctcaccgaca
     2821 cgatcgccaa ggcgttcgac gaggtgttcg ccagcgccca ggagggcgtg gacgtcgagg
     2881 tcagggccga ggcctccgac ctggccgcgg gaggcgaccg cgagcgcatc ccggaggagc
     2941 tcctctccgt gatccagagg gtcgagggcg tggaccgggt cgagggcggc gtcaccggct
     3001 acgcccagat cgtggacgcg agcggcgaag cgaccggcac cacgggcgcc cccacgttcg
     3061 ggatctcgtg gcccacctac gaggggttct cggcgctctc gatccgcgag ggccgggccc
     3121 cgctcacggg gggcgaggtg gcgatcgacg ccgccaccgc ggagcgtcac ggcttcgtca
     3181 tcggcgaccg ggtcaccgtg ctcttccagg ggccgcagga ggaattccag gtggtgggca
     3241 tcgcggggtt cggcgacgcg gacaacctcg cgggcgccac gatggccgcg ttcgacgtcg
     3301 ccaccgcgca gcgggtcctc ggggccgagg acaccttcga ctccatcttc gtggcggccg
     3361 acgagggggt gggggcagcc gagctccggg accggatctc cgaggtcctc ccccaggggg
     3421 ccgaggcgca gacgggggct gcggtggccg cagagcaggc cgacgcgatc aaggatgcgc
     3481 tcggcttctt caacaccttc ctcctggtgt tcgcggcgat cgcgctcttc gtgggagcct
     3541 tcatcatctt caacacgttc aacatcctcg tcacacagcg gacgcgggag ctggcgctgc
     3601 tgcgagccct gggagcaagc ccgagacagg tcacgctctc ggtgctcacc gaggcgggga
     3661 tcgtggggct cgccgcctcg gtcgtaggtc tcgggctggg gatcctggtg gcgttcggcc
     3721 tgagggccct cctggccggc ttcgggatcg acctcccgtc ggcgagcctg cagatcctcc
     3781 cgagaacgat cgcggtggcg ttcctcgtgg gcatcctggt cacgctcgtc gcggcgctca
     3841 tgcccgcgcg cagagccgcc cgcatctcgc ccatcgccgc catgaaggcc gtggagacgg
     3901 tgcggcctct cgactcgagg cgccggatgg tcatcggcgg ggcactggtg gcgggagggg
     3961 tcgccctcgg gctcgtgggc ctgttcggcg gaggcgacag cgcgctgcag gcggtcggga
     4021 tcggggtggc cgccaccttc atcggcacgg ccatcctgag ccccctcatc gctcagccgc
     4081 tggctcgagc gatcgggtcc cctctccccc ggctcgccca cctcccgggc aagctgggcc
     4141 gcgagaacgc gatgcgcaac ccccggcgaa cggcatccac ctcggcggcg ctgatgatcg
     4201 gcctggccct cgtagggacg ttcctgatct tgggctcgtc gctgaagacc tcggtgggcc
     4261 agac
//
LOCUS       DASUTW010000041         9925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1179512, whole genome shotgun sequence.
ACCESSION   DASUTW010000041 DASUTW010000000
VERSION     DASUTW010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9925
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179512"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..739)
                     /locus_tag="VF058_01420"
     CDS             complement(32..739)
                     /locus_tag="VF058_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206995.1"
                     /translation="MIEAGTPVAWGEGLVWLGGVIAAGFLISWALTDLLRMHRALYVG
                     LLTLLAWGLTIGYVLRTGSGVTFWVERWPWGLLGALVAGAFLAMMVSRRPAEREADRM
                     GPGDALWEGVVYGSAEGLLLSVLPVLITWQLLGALGWGDAWRGLAALAASVVVIVAHH
                     LGYPEYRGRTIVSPVIGCAVLSIAYLATGSPIAAIGGHIVLHLAMRLRGMELPPHTES
                     VLGRDRAGGTLAGARSG"
     gene            875..1204
                     /locus_tag="VF058_01425"
     CDS             875..1204
                     /locus_tag="VF058_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6206996.1"
                     /translation="MDPREERVARNESTSREINEGIQEAHEDAGPTEQIRMLCECGHR
                     TCERVIAITPAEYERVRSDGRRFAVVLDHVIPDVEEIVEETDRYVVVVKREGTPADVA
                     LEEDPRS"
     gene            1249..2634
                     /locus_tag="VF058_01430"
     CDS             1249..2634
                     /locus_tag="VF058_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX6206997.1"
                     /translation="MEVRADSPPRVLADRYEVGELLGAGGMASVYRAHDRILQRPVAV
                     KVLDASLAADDRFLRSFVREARAAAGLIHPNVVTVFDTGVDDDQHFILMELVEGSTLQ
                     QVLGREHRLGPERARRIAIDVARALTVAHGRGIVHRDVKPANVLIGEDGRPRVADFGI
                     ARPTDQADESRTIFGTAAYLSPEQARGRPTTPASDVYSLGCVLYEMLAGRRPFTGDSP
                     VSVAVKHLRYTPPPVEALAPDVPPALAAVVHKAMQKDPRRRFPDAGAMLAALEGAATV
                     SGLPQRRRPGILRWVAASVSLALIAVLAPALIGAGGDAPEPGARVIAEGRGRGPARVE
                     VPSVTGMSRQEAGRALRRADLVPIFLGAPLHESASHVVGSQDPQAGASLAAGSFVTLE
                     LVPADADVAPPAGSGSSEKKRRGSSGSGRSAGGRSGEDRPEASPSPSPTPSPSPTPTP
                     SPSPTPTEGVR"
     gene            2640..4010
                     /locus_tag="VF058_01435"
     CDS             2640..4010
                     /locus_tag="VF058_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008272615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX6206998.1"
                     /translation="MFLERFHEEKLGHASYLVGSQDEGEALLVDPRRDVDPYLEAADR
                     RGVRIGCALDTHGHNDYLSGLSEIAARTEAEILASAHGEARYPHRPLKDGQSLEVGEV
                     VVEAMHTPGHTPEHLALLVRDASVDELEPLMLLSGGSLLVGDVARPDLLGGRDEAVEA
                     ARVLCRTLEERILTLPDHVEVHPTHVSGSLCAGSIGSRLTTTIGYERRTNPSLARPED
                     CVSLEDLPAVPPYWRRMRSQNLEGVPLLGTVAPPPAFRPEEVERRLAEGALALDARAP
                     EAFAEGHIPGALSAGLDGSFPTWAGTVLPEGARVVLVLDDPGRVMEAAWHLLRIGYDP
                     PLGYLEDGMEAWRAEGRPVREMPVVDVHELRRRLDDVAVLDVRQPGEWRDGHIAGATH
                     VTGAELQRRLDEVPSRRPLAVLCGTGYRSSASASLLLREGFDDVWNVLGGSAAWDAAG
                     YPTVRA"
     gene            4007..4426
                     /locus_tag="VF058_01440"
     CDS             4007..4426
                     /locus_tag="VF058_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX6206999.1"
                     /translation="MSDPACVFCRIVSRDAPSLTIYEDEATMAFMDINPATDGHCLVI
                     PKRHAPDVWTLPEEDGVAVWRSVRRLAAAVRDALRADGLNILQSNGRAALQTVFHYHV
                     HVIPRYVGDGIHVPLMRAPGDRSRLGEYADLIRSELT"
     gene            complement(4423..5292)
                     /locus_tag="VF058_01445"
     CDS             complement(4423..5292)
                     /locus_tag="VF058_01445"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6207000.1"
                     /translation="MAVLAFCGLGHMGAPMARRLLQAGHEVAVWNRTEEKARPLVEAG
                     ARAAASPADAAEGAEAAFTMLADPGAVREVVLGTGGLAESLRGDATLIEMSTIGIRAV
                     REIAEALPEGVAVLDTPVLGSVAQAEEGTLRIFVGGPSDLVDRWAPTLRAMGTPQRFG
                     DLGAGAAMKLVVNSTLGALMAGLGEALALADALGVDEEAALDVLEGSPIGATTRSKRD
                     KIASGRYPPNFRLGLAAKDLRLVVEEAVEAGARLPVAEAVLARLEAARDAGLGGLDYS
                     AVIPHVRGRPATG"
     gene            5333..5680
                     /locus_tag="VF058_01450"
     CDS             5333..5680
                     /locus_tag="VF058_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207001.1"
                     /translation="MSDEYAPLPPVHPVLAAMAGGALWGAMGYAILWGLSPIVVQRRF
                     VVSAIGTIAFLPVRLVLWTLRWIESIEGRPFEFAHSSWWIGALAAVVGAGIGIALALT
                     ARTAIRRLRPTRD"
     gene            5767..6666
                     /locus_tag="VF058_01455"
     CDS             5767..6666
                     /locus_tag="VF058_01455"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX6207002.1"
                     /translation="MPDAPEAPRGWRALPTLAVRTVRDSFQDRIFGLGAEVAFFFLLS
                     FPPALLTILGTLGYVSQVLGEDVAAAMRDQILSVAGSFLTQETVTETVRPLVNRLLAE
                     GRADVASFGIVLTLWAASRATTRLIEAVAIAYDEEDERSAVRRRLLSLGMTVGTIVAL
                     VLVIPILVAGPRLLEALAEPLGLTAAVAAGWQVLYWPVVGVIGIGLLSLFYHLASPLE
                     TRWRRDVPGAFAAAGVWLLGGLGLRVYLSFSLDSGAYGPLAAPIAVLLWMYVTALAVL
                     LGAEVNAEIEKMWPDRPRKKRDA"
     gene            complement(6663..7049)
                     /locus_tag="VF058_01460"
     CDS             complement(6663..7049)
                     /locus_tag="VF058_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX6207003.1"
                     /translation="MAGRRGDRLRAGALEAFALGLALLGACEDSPRDAGPQRVRVEMR
                     EFAFDPSEIALRAGVLVDLRLRNVGRVEHDWTLLAPGGEEVSYVYVRPGRRGRDRFTL
                     DEGTYDLLCTIEGHAEAGMVGTVVAG"
     gene            complement(7022..7285)
                     /locus_tag="VF058_01465"
     CDS             complement(7022..7285)
                     /locus_tag="VF058_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207004.1"
                     /translation="MAGWLEFRQCPGCGFDPATTEGEKGCHYFDCPYLPAELDVLCPQ
                     CRFDFYTGEGNPSCDDPATCAEGATARANVENVRRWRAGAAIG"
     gene            complement(7371..8552)
                     /locus_tag="VF058_01470"
     CDS             complement(7371..8552)
                     /locus_tag="VF058_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207005.1"
                     /translation="MRFSGPSAAAQVLARIWLLAAAASLLLPAADRLGWWLPIHLALA
                     GAAGSAVSGTLPLFANALAGAHDLPQRWDPLLLVTAGAASIAIGRPLAITWLLAVGGG
                     AYALGSLRLTWLVLTARRAGSNRRHSAALFAYAGAALSLTLGAILGALLGAGSVGGAA
                     ILDVRAAHVTLNAEGFLALAVAGSLLVLVPITLRVRAPDATGRLVVGSLGLGVILQAA
                     GHVASWTPLVAVGAGAALVGAADLVRVAAGSVARGPRMPEPGAALHLLSVPLWLLGVT
                     AAALILALTGSAGWPLPLVAALTLGVFVQALLGAWSYLLPVSDPAGSEARRARLERTR
                     RWAWPRAAVFNAGAALVALTAAGWLPSSLGTIGLVGAAAAALAAIVLASLRAPERAEA
                     T"
     gene            complement(8556..9068)
                     /locus_tag="VF058_01475"
     CDS             complement(8556..9068)
                     /locus_tag="VF058_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010593700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEX6207006.1"
                     /translation="MAELAELPTPEEAEEKKLVIMAWSGDLDKVWPTLILATTGAAMG
                     MRTTVFFTFWGLFPLVKNDVRITGENWMQKMLSMMNRGGTEHLKLGKMNFAGMGPWMM
                     KKLAAEHKVASPQELLELARDMGVRLVPCQMTMDMLGLRREDLIDGIDDPAGATTALL
                     EAQDAVTLFI"
     gene            complement(9074..9304)
                     /locus_tag="VF058_01480"
     CDS             complement(9074..9304)
                     /locus_tag="VF058_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX6207007.1"
                     /translation="MKTIAKTLDLKGLSCPLPVVKTAQAMKEAASGDLIEVLATDPGS
                     VPDFKAWAETTGNELVEQTEQEGVYRFLIRKR"
     gene            complement(9301..>9925)
                     /locus_tag="VF058_01485"
     CDS             complement(9301..>9925)
                     /locus_tag="VF058_01485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEX6207008.1"
                     /translation="SPLNRAFTIESASKLVQPIMERRGIGLETFFNVEAVDPAAHVVE
                     SLEGEKAEYDLLVLVPPHRGQEVVASSGLGDAGGWMPTDPATLQHKEHERIFAIGDAT
                     NLPISKSGSTAHFEGPVVASRIASLIRGTAPKASYGGRVMCFLETGGGRSTVLRFDYE
                     RPPVPPKPSRRWHAAKWLFNRLYWNTVPQGRIPEFLGSPRSKEGSEA"
BASE COUNT         1311 a         3727 c         3557 g         1330 t
ORIGIN      
        1 ctctcgacgc gaccatcgct ccctcctcct ctcagccgga tctcgccccc gccagcgtcc
       61 ctccggctcg gtctcggccg aggacgctct cggtgtgcgg cgggagctcc atgcccctca
      121 gccgcatggc gaggtgcagc acgatgtggc cgccgatcgc cgcgatcggg ctgccggtcg
      181 ccaggtaggc gatgctcagc accgcgcagc cgatcaccgg cgagacgatc gtccgccccc
      241 ggtactcggg atagccgagg tggtgggcga cgatgaccac gacgctcgcg gccagggccg
      301 ccaggcccct ccaggcatcg ccccacccga gcgccccgag cagctgccac gtgatcagca
      361 cgggcagcac cgagagcagc aggccctccg ccgacccgta gaccaccccc tcccacaggg
      421 cgtcgccggg gcccatccgg tccgcctcac gctcggcggg acggcgagag accatcatgg
      481 ccaggaacgc tcccgccacc agggcgccca gcagacccca cggccagcgt tcgacccaga
      541 acgtcacgcc gctcccggtc cggaggacgt agccgatcgt gaggccccac gcgagcaggg
      601 tgagcaggcc cacatagagc gcccggtgca tgcgcaggag gtcggtgagg gcccacgaga
      661 tcaggaagcc ggcggcgatc acgccgccga gccacaccag cccctcgccc cacgcgacgg
      721 gcgtgccggc ttcgatcatg ccttccacct cctcggttgc ccggtcccac gctagggact
      781 cctcgcgctc cctccgaggg ccgggggtcc ccctcagcgg gtgaccttcg ggctcgctcc
      841 attaggacct tgatcacctg ggtacgatgc aggcatggat ccgagggaag agcgcgtcgc
      901 gcgcaacgag tcgacctcac gggagatcaa cgagggcatc caggaggccc acgaggatgc
      961 ggggccgacc gagcagatcc ggatgctgtg cgagtgcggc caccggacgt gcgagcgagt
     1021 gatcgcgatc acgcccgccg agtacgagcg cgtccggagc gacggacggc gcttcgccgt
     1081 cgtcctcgac cacgtgatcc ccgacgtcga ggagatcgtg gaggagaccg accgctacgt
     1141 ggtggtcgtg aagcgcgagg ggacgcccgc cgacgtcgcc ctcgaggaag acccccgcag
     1201 ctaggtccgg cgtttcctgc gcggccccgt cgggtatccg gcgatgggat ggaggtgcgc
     1261 gcagactcgc cgccacgcgt gctcgccgac cgctacgagg tcggcgagct tctgggtgcg
     1321 ggaggcatgg cgagcgtgta ccgggcccac gaccgcatcc tccagcgtcc cgtcgccgtc
     1381 aaggtcctcg atgcgtcgct cgccgccgac gaccggttcc tgcgcagctt cgtccgggag
     1441 gctcgcgcgg ccgcgggtct gatccacccc aacgtggtga cggtattcga caccggggtg
     1501 gacgacgatc agcacttcat cctgatggag ctggtcgagg ggagcacgct ccagcaggtc
     1561 ctgggccgcg agcatcgcct ggggccggag cgggcccgcc ggatcgcgat cgacgtggcc
     1621 agggccctga cggtcgccca cggacggggg atcgtccacc gggacgtgaa gcccgccaac
     1681 gtgctgatcg gcgaggacgg gcgtccccgc gtggcggact tcggcatcgc tcgccccacc
     1741 gaccaggccg acgagagccg cacgatcttc gggaccgccg cctacctctc gcccgagcag
     1801 gccagaggcc gtcccaccac gcccgccagc gacgtgtact cgctcggctg tgtcctctac
     1861 gagatgctcg ccggccgtcg cccgttcacc ggggacagcc ccgtgtcggt ggccgtgaag
     1921 cacctccgat acacgccgcc gcccgtggag gccctggccc ccgacgtgcc tccggcgctg
     1981 gcggcggtcg tccacaaggc catgcagaag gatccacgcc gccgcttccc cgatgcggga
     2041 gccatgcttg ccgctctgga gggcgccgcc acggtctcgg ggcttcccca gcggcgccgc
     2101 ccggggatcc tgcggtgggt ggctgcctcg gtgagcctgg ccctgatcgc cgtccttgcg
     2161 ccggcgctga taggggccgg cggcgacgcg cccgagcccg gggcgcgcgt gatcgccgaa
     2221 ggacgcgggc ggggacccgc ccgtgtcgag gtcccgtccg tcaccggcat gagccggcag
     2281 gaggccggcc gggccctccg ccgagctgac ctcgtcccga tcttcctcgg ggccccgctg
     2341 cacgagagcg cgtcccacgt cgtgggctcc caggacccgc aggcgggtgc cagtctggcc
     2401 gcggggtcct tcgtcacgct ggagctcgtg ccggcggacg ccgacgttgc tccgccggcg
     2461 gggagcggct cgagcgagaa gaagcggcgg ggttcgtccg gctcggggag gtcggccggc
     2521 gggcgctccg gcgaggaccg gcccgaggcc tcgccctccc cctcgcccac ccccagcccg
     2581 agcccgacgc ccacgccctc cccctcgccc accccgaccg agggcgtacg ctaggccgga
     2641 tgttcctcga acggttccat gaggagaagc tcgggcacgc ctcctacctc gtcgggtcgc
     2701 aggacgaggg cgaggcgctg ctggtcgatc ccaggcgcga cgtcgacccg tacctcgagg
     2761 ccgcggatcg caggggcgtc cggatcggct gcgccctcga cacgcacggg cacaacgact
     2821 acctgagcgg gctctccgag atcgcggccc ggaccgaggc cgagatcctg gcctccgccc
     2881 acggggaggc ccgctacccg caccggcctc tcaaggacgg gcagtcgctc gaggtgggag
     2941 aggtcgtcgt ggaggcgatg cacacgcccg gccacacccc cgagcatctg gcgctgctgg
     3001 tgcgcgacgc ttcggtggac gagctggagc cgctgatgct gctctcgggc ggctcgctgc
     3061 tggtgggcga cgtggcccgt ccggacctgc tgggaggccg tgacgaggcc gtggaggccg
     3121 cccgcgtcct gtgccggacc ctggaggagc gcatcctgac gctccccgac cacgtggagg
     3181 tgcacccgac gcacgtctcg ggatcgctct gtgcggggag catcgggagc cgcctcacca
     3241 cgacgatcgg gtacgagcgg aggacgaatc cctcgctggc ccggcccgag gactgcgtct
     3301 cgctggagga cctcccggcc gtccccccgt actggcgcag gatgcggtcg cagaacctgg
     3361 agggcgtccc cctgctgggc acggtggcgc cgccgcccgc cttccggccg gaggaggtcg
     3421 agcggcggct ggcggagggg gccctggccc tcgacgcacg cgcccccgaa gcgttcgccg
     3481 agggccacat ccccggcgcg ctgagcgccg ggctggacgg ctcgttcccc acgtgggcgg
     3541 gcaccgtcct gcccgagggc gcccgcgtcg tgctggtgct ggacgacccc gggcgcgtca
     3601 tggaggcggc ctggcacctc ctgcggatcg ggtacgatcc gccgctggga tacctcgagg
     3661 acggcatgga ggcctggcgg gccgagggga ggccggtgcg cgagatgccc gtggtggacg
     3721 tccacgagct ccgtcgccgc ctggacgacg tcgcggtgct ggacgtgcgc cagccggggg
     3781 agtggcggga cggccacatc gccggcgcga cccacgtcac cggcgcggag ctccagcgcc
     3841 gcctggacga ggtcccgagc cgccgaccgc tggccgtcct ctgcgggacc ggctaccggt
     3901 cctccgcgtc ggccagcctg ctcctgcggg agggcttcga cgacgtctgg aacgtcctgg
     3961 gcgggagcgc cgcctgggac gcggccggat accccacggt ccgagcgtga gcgacccggc
     4021 ctgcgtcttc tgccggatcg tgtcccgcga cgccccctcg ctcacgatct acgaggacga
     4081 ggccacgatg gccttcatgg acatcaaccc ggccaccgac ggccactgcc tcgtgatccc
     4141 gaagcgccac gcgccggacg tctggaccct gcccgaggag gacggggtcg ccgtgtggcg
     4201 gtcggtccgc cggctcgcgg cggccgtccg agacgccctc cgggccgacg ggctgaacat
     4261 cctccagagc aacgggcggg cggcgctgca gaccgtcttc cactaccacg tgcacgtgat
     4321 cccccggtac gtgggggacg ggatccacgt gccgctgatg cgggcgcccg gcgaccggag
     4381 ccggctgggc gagtacgctg acctgatccg gagcgagctg acctagccgg tcgccgggcg
     4441 cccccggacg tgggggatca ccgccgagta gtcgaggccg ccgaggccgg cgtcgcgggc
     4501 ggcctccagg cgggccagca cggcctccgc gaccgggagc cgggcgccgg cctcgacggc
     4561 ctcctccacc accagccgga ggtccttcgc cgcgagcccc agccggaagt tcggggggta
     4621 gcggccggac gcgatcttgt ctcgcttcga gcgcgtggtg gcgccgatcg gcgagccctc
     4681 cagcacgtcg agggcggcct cctcgtccac cccgagggcg tcggccagcg ccagggcctc
     4741 gccgagcccg gccatcaggg ctcccagcgt cgagttcacc acgagcttca tggccgcccc
     4801 cgcccccagg tccccgaacc gctgcggcgt ccccatcgcc cggagcgtcg gcgcccagcg
     4861 gtccacgagg tcgctggggc cgcccacgaa gatccggagc gtcccctcct cggcctgggc
     4921 cacgctgccg agcacgggcg tgtcgagcac ggccacgccc tcggggagcg cctccgcgat
     4981 ctccctcacg gcccggatcc cgatcgtgga catctcgatc agcgttgcgt ccccgcggag
     5041 actctcggcc agccccccgg tcccaaggac gacctcccgg accgcgccgg ggtcggccag
     5101 catcgtgaag gccgcctctg ctccctccgc ggcgtcggcc ggcgaggccg ccgcccgggc
     5161 ccccgcctcc accagcggcc gggccttctc ctcggtccga ttccagacgg ccacctcgtg
     5221 cccggcctgc agcagccgcc gggccatggg ggcgcccatg tgccccagtc cgcagaaggc
     5281 gagcacggcc acggcgacag cctacccgcg accgcgtggg aggatggcgg ggatgtcgga
     5341 cgagtacgcg ccgctccccc cggtccaccc cgtcttggcc gcgatggccg gcggcgcgct
     5401 ctggggggcg atgggctacg ccatcctgtg gggcctgtcg ccgatcgtgg ttcagcggcg
     5461 cttcgtcgtg agcgcgatcg ggacgatcgc cttcctcccg gttcggctcg tcctctggac
     5521 gctccgctgg atcgagagca tcgaagggcg gccgttcgag ttcgcccact ccagctggtg
     5581 gatcggagcg ctggcggcgg tcgtgggggc cgggatcggg atcgcccttg ccctgacggc
     5641 tcgcaccgcg atccgccggc ttcggccgac ccgggactga cggccgcgcg tgtggcggct
     5701 gatcgccggg ggggctcgag gaccgcggcg cttgcaccgt gaccgtcgtg ggatgatcga
     5761 ggggcggtgc cggacgcacc cgaggctcct cgcggctggc gcgcgctgcc cacgctggcg
     5821 gtgcgcaccg ttcgcgactc cttccaggac cggatcttcg gcctcggggc cgaggtggcc
     5881 ttcttcttcc tcctctcgtt ccctccggcg ctgctgacga tcctggggac gctgggctac
     5941 gtctcgcagg tcctggggga ggacgtggcg gccgcgatgc gggaccagat cctgagcgtc
     6001 gcgggcagct tcctcacaca ggagacggtg accgagacgg tgcgaccgct cgtgaaccgg
     6061 ctgctggccg agggaagagc cgacgtggcg tcgttcggga tcgtgctcac gctgtgggcc
     6121 gcctcccggg ccacgacccg cctgatcgag gcggtcgcga tcgcctacga cgaggaggac
     6181 gagcgctcgg ccgtgcgccg ccgcctcctc tctctgggga tgacggttgg gacgatcgtc
     6241 gccctcgtgc tcgtgatccc gatcctcgtc gccggccccc gcctgctcga ggcgctggcc
     6301 gagcccctgg ggctgaccgc agccgtcgcc gccgggtggc aggtcctgta ctggccggtc
     6361 gtcggggtga tcggcatcgg gttgctctcg ctcttctatc atctggcctc cccgctggag
     6421 acccggtggc gtcgggacgt cccgggggcc ttcgcggcgg cgggcgtgtg gctcctcggg
     6481 gggctggggc tgcgcgtgta cctgagcttc tcactggact cgggggccta cgggccgctg
     6541 gcggctccga tcgcggtcct cctctggatg tacgtcacgg cgctggcggt gctgctgggg
     6601 gccgaggtca acgccgagat tgagaagatg tggccggatc ggccgaggaa gaagcgcgac
     6661 gcttagcccg cgacgacggt tcccaccatg ccggcctcgg cgtgaccctc gatcgtgcag
     6721 aggaggtcgt aggtcccctc gtcgagcgtg aagcggtctc ggccccgccg accgggccgg
     6781 acgtacacgt agctcacctc ctctccgccc ggggccagga gggtccagtc gtgctcgacc
     6841 cggcccacgt tccggagtcg gaggtccacg aggacgccgg cccgcagcgc gatctcggac
     6901 ggatcgaagg cgaactcacg catctccacg cggacgcgct gcgggccggc gtccctcggc
     6961 gaatcctcgc aggcgcccag cagggcgagc cccagcgcga acgcttcgag cgcgcccgcc
     7021 ctcagccgat cgccgcgccg gcccgccacc gccggacgtt ctcgacgttc gcccgagccg
     7081 tcgcgccctc cgcgcacgtc gcggggtcgt cgcaggacgg gttgccctcg ccggtgtaga
     7141 agtcgaaccg gcactgggga cagaggacgt ccagctcggc cggcaggtag gggcagtcga
     7201 agtagtggca gcccttctcg ccctcggtcg ttgcggggtc gaagccgcag ccgggacact
     7261 gacggaactc cagccagcct gccatcgctc ctcctttcgt tgccccaccg taggagggcg
     7321 ccccggaagg tcccagggcc ttccggcccc cgggcggcgg gccggtcggc tcaggtggcc
     7381 tcggcccgct cgggcgccct cagcgaggcc aggacgatcg ccgccagcgc ggcggcggcc
     7441 gcgcccacca ggccgatcgt ccccagcgag gaggggagcc acccggccgc agtcagcgcg
     7501 accagcgcgg ctccggcgtt gaagaccgcc gctcggggcc aggcccaccg gcgggtccgc
     7561 tccaggcgcg ccctccgcgc ctccgacccc gcggggtccg acacgggcag gaggtacgac
     7621 caggctccca gcagggcctg gacgaagacg ccgagcgtga gcgccgccac gagcggcagc
     7681 ggccaccccg cggacccggt gagtgcgagg atcagcgcgg ccgctgtcac gcccaggagc
     7741 cagagcggga ccgagaggag gtgcagcgcc gcccccggct cgggcatccg cggccccctg
     7801 gccacggacc cggccgccac gcgcacgagg tcggcggcgc ccacgagcgc ggccccggcg
     7861 ccgacggcca cgagcggcgt ccaggatgcg acgtgtcccg ccgcctggag gatcaccccg
     7921 agcccaaggc ttcccacgac gagacggccg gtggcatcgg gtgcccgcac gcgcagcgtg
     7981 atggggacca gcacgagaag ggatcctgcg acggccaggg ccaggaaccc ctccgcgttg
     8041 agcgtcacgt gggcggcgcg gacatcgagg atggccgcgc cgcccacgct ccccgctccc
     8101 agcagcgccc cgaggatggc ccccagcgtc agcgagagcg ccgcccccgc gtaggcgaag
     8161 agggccgccg agtggcgccg gttgctcccc gcgcgccggg cggtcagcac gagccaggtg
     8221 aggcgaaggg accccagcgc gtaggctccg ccgccgaccg ccaggagcca cgtgatcgcc
     8281 aggggcctcc cgatcgcgat cgaggccgcc ccggccgtga cgagcaggag cggatcccag
     8341 cgctggggga ggtcgtgcgc gcccgccagc gcgttcgcga agagcgggag ggtgccggag
     8401 acggcggacc ccgccgcccc ggccagcgcg aggtggatcg gcagccacca gcccaggcgg
     8461 tcggccgcgg ggagcagtag cgaggccgcc gccgcgagga gccagatccg ggccaggacc
     8521 tgggctgcgg cggacggccc cgagaacctc atggatcaga tgaacagggt gaccgcgtcc
     8581 tgcgcctcca gcagcgccgt cgtcgctccg gcggggtcgt cgatgccgtc gatcaggtcc
     8641 tcacggcgga ggccgagcat gtccatcgtc atctggcacg gtaccagccg gacgcccatg
     8701 tcgcgcgcca gctccaacag ctcctggggg ctggccacct tgtgctccgc cgcgagcttc
     8761 ttcatcatcc acgggcccat ccccgcgaag ttcatcttcc ccagcttcag atgctcggtg
     8821 cccccgcggt tcatcatcga gagcatcttc tgcatccagt tctcgccggt gatccggacg
     8881 tcgttcttca ccagggggaa cagcccccag aacgtgaaga agacggtcgt gcgcatcccc
     8941 atcgcggcgc cggtcgtggc caggatcagc gtgggccaga ccttgtccag gtccccgctc
     9001 caggccatga tgaccagctt cttctcctcg gcctcctccg gggtggggag ctccgcgagc
     9061 tctgccatct cggtcacctc ttcctgatca ggaaccggta cacgccctcc tgctccgtct
     9121 gctccacgag ctcgttcccg gtcgtctccg cccaggcctt gaagtcgggg accgaccccg
     9181 ggtcggtggc caggacctct atcaggtctc cggaggccgc ctccttcatg gcctgcgcgg
     9241 tcttcacgac cggcagcggg caggacaggc ccttcaggtc cagcgtcttc gcgatcgtct
     9301 tcatgcctcg ctcccttcct tgctccgggg gctccccagg aactcgggga tccgcccctg
     9361 gggcacggtg ttccagtaca ggcggttgaa gagccacttc gccgcgtgcc agcgccgcga
     9421 cggcttgggg gggacgggcg ggcgctcgta gtcgaaccgc agcaccgtcg acctcccccc
     9481 gccggtctcc aggaagcaca tcacccggcc cccgtaggag gccttgggcg ctgtgccccg
     9541 gatcagcgac gcgatcctgc tcgccacgac gggcccctcg aagtgggccg tcgagccgct
     9601 cttgctgatc ggtaggttcg tggcgtcgcc gatggcgaag atccgctcgt gctccttgtg
     9661 ctggagcgtg gcggggtcgg tcggcatcca gccccccgcg tcgcccagcc cggagctcgc
     9721 cacgacttcc tgaccccggt ggggcgggac caggacgagc aggtcgtact cggccttctc
     9781 gccctccagc gactccacca cgtgggccgc cgggtccacg gcctcgacgt tgaagaaggt
     9841 ctccaagccg atgccgcgac gctccatgat cggctggacg agcttcgagg ccgactcgat
     9901 cgtgaacgcc cggttgagcg gcgag
//
LOCUS       DASUTW010000042         8189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_402373, whole genome shotgun sequence.
ACCESSION   DASUTW010000042 DASUTW010000000
VERSION     DASUTW010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8189
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402373"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VF058_01490"
     CDS             complement(<1..405)
                     /locus_tag="VF058_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX6207009.1"
                     /translation="MASFLKNPLREELDPGPEPDPRPFHRKLPGYEPSPLREAPSIAE
                     RLGVGEVWVKDESSRLGLPAFKILGASWAAYRALVRRLGEEPEWETIEELAERFEPLK
                     PLTLATATDGNHGRAVARMARWLGLEARILVPA"
     gene            complement(405..2129)
                     /gene="ade"
                     /locus_tag="VF058_01495"
     CDS             complement(405..2129)
                     /gene="ade"
                     /locus_tag="VF058_01495"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEX6207010.1"
                     /translation="MTAFTDRLARRLAVARGDEPADLLLKGGRVLSVFTGELLEANVA
                     VAGEHVAGVGDYSDAAEVEDCSGLILLPGFIDAHMHLESTKLMVDQFARAALPWGTTT
                     VILDPHEIANVFGLKGVRAILASAGAVPLDFYVMVSSCVPASAFESGGATVTAEDIAR
                     FLAEEPSAIGVAEMMDYPSVVAGAPEALAKIEAAGSGHVDGHAPGLSGRDLNAYLAAG
                     VGSDHEATTLDEAMEKRRLGMWILIREGSAARNLEGLAPMVLEHGPTNCCLCTDDREP
                     DQLLREGHINDVMRKAVALGIRPADAVVMGTLNAARYHRLREHGAVAPGYVADVVAVE
                     DLEAFRPVKVWKRGRLAAEDGEAVDIAEIEAPAWMRGSVRVPTLTAADFRVSADGRVR
                     AIGVRDGSIVTEALVVDPRTEDGHAVSDVDRDLAKVAVVERHRETGRIGLGFVRGFGL
                     QRGALASTHAHDAHNVVVVGVDDADMASAVNRLAEIGGGQVAVAEGEVVAEVPCPIGG
                     LLSDLRAEEVAAAFERLERAAHDLGATLASPFMAMSFLALSVIPELKITDRGLIDTVS
                     FELVPLEV"
     gene            2245..3708
                     /locus_tag="VF058_01500"
     CDS             2245..3708
                     /locus_tag="VF058_01500"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEX6207011.1"
                     /translation="MTRHALRLLLLGAMCSSFLVPAGPAAAGTFRVTILGDPSPGVCD
                     ARCSLREAVIAANASPGPDVIRLPKGRVELSRAGAGEEAGATGDLDVTDTLTIVGAGM
                     GRSVVDGNDLDRVFDASSGTFVLRRLTVTGGHVETGGATAFGGGIRGWGALVRLREVA
                     VRGNDAADGGGGVAGFTVEIQRSLIAGNRADSAGAVDGSTVKIADSLLLGNRATLSGG
                     AMALFFGSTVELRNTTVAGNTSGHNAGGIGINSGSALLLNVTVHGNHTDGDAGAPGGN
                     GGGVQVNAGSTMEIRNTIISGNTDGAPTAAHDCAGDLTSLGSSLVRDQNGCSLTGASG
                     DLPAGTDPRLRPLANNGGATRTLALPPGSPARNAGAACLSKDQRGVPRSLGGACDIGA
                     YELVSCFGRIVNRVGTQGNDVLRGTGRKDGFLGFGGRDRLIGRGGSDGMCGNGGRDTL
                     EGGPGRDRLHGGPGRDLCVGGPGRDRARSCERRREIP"
     gene            3773..5737
                     /gene="acs"
                     /locus_tag="VF058_01505"
     CDS             3773..5737
                     /gene="acs"
                     /locus_tag="VF058_01505"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX6207012.1"
                     /translation="MATEDRKDIAALLEEHRLIEAPRGFREQAVVSDEGIYGRAAEDP
                     EGFWAEQAGHLDWIEPWDEVLRWDPPWVRWFEGGRLNISANCLDRHLEAHGDRVAYHW
                     KGEPGDERTITYRELHEEVSRFANALKRLGVAKGDRVAIYLGMIPELPVAMLACARIG
                     AVHSVVFGGFSADSLRDRINDAQASVLVTADGGWRRGQIVPLKENADQAVAETPSIEQ
                     VVVVRRTGEEVPFREGRDLWYDELIAAESPDCPAEPMEAEDPLYILYTSGTTGKPKGI
                     QHTTGGYLVGVTATHRMIFDLHPETDVYWCAADIGWVTGHSYIVYGPLANGTTSVMYE
                     GAPDFPDKDRLWSIVAKHGVTIFYTAPTAIRAFMKWGPEHVRKHDLSSLRLLGTVGEP
                     INPEAWMWYWEHVGGERCPVVDTWWQTETGMILITPLPGITPLKPGSATRAFPGVEAD
                     VFDEAGGSVPPGAGGYLVLKRPWPAMARTIHGDPDRYVEQYFSKYGPSVYLAGDGAKR
                     DEDGYFWLLGRIDDVMNVAGHRLSTYEIESALVDHPSVAESAVVSRPDPDVGEAIVAY
                     VTLKGHIEGDESLIDELREHVAEKIGKIARPHAIVLTDDLPKTRSGKIMRRLLRDVAH
                     GRDLGDVTTLANAEVVEEIRELATSSPPEE"
     gene            5734..6942
                     /locus_tag="VF058_01510"
     CDS             5734..6942
                     /locus_tag="VF058_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6207013.1"
                     /translation="MTEAPGRRRALALLTLAMVLGLATWFSASAVIPQLREEWSLGPT
                     AAAWLTIAVQLGFVAGALVSAVLNLADVHSARTVIVVSGFGAALANLGLVAADDAALG
                     IPLRFLTGFFLAGVYPPGMKVIATWYREGRGYALGVLVAGISLGSAMPHLVNGLGGLS
                     WRTVIVITSILTASGGVIARYLVGDGPFPFPRAVFDPRQAGRVFRNRGVRLATFGYFG
                     HMWELFAMWAWFLVFFTESLETAGRDPTVLAPLATFAVIGSGALGCWAAGILGDRWGR
                     SRTTSAAMAISGACALTIGLLFGRSPWLVLALGIVWGITVVADSAQFSAAVTETANTA
                     YVGTAVTLQTALGFTLTVATIWLVPIVRDALSWWWAFAMLALGPALGILAMQRLQRSP
                     EAFRIAGGRG"
     gene            7038..8165
                     /locus_tag="VF058_01515"
     CDS             7038..8165
                     /locus_tag="VF058_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017290640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX6207014.1"
                     /translation="MIPLTVRRLLDREGVDALIRVLRERGYTVVGPRLREQAIVYDEI
                     GSVEDLPEGWTDEQEGGTYRLHRRDDAALFGYAVGPHSWKRELFPPETLLARIRRQNG
                     EVEFEQPEPDAPRYAFLGVRACEVAAIEIQDRVFMAEGAEDPTYAARRRDALVIAVQC
                     GSPSGTCFCVSMDTGPRVEDGYDLALTEILEGGHRFLLEVGSERGAEIADALPSSPAA
                     DEDVGAADAVVERAAASMGRRMETDGIKELLYRNLEHPRWEDVAQRCLACTNCTMVCP
                     TCFCSTIEDSTDLSGETAERHRRWDSCFTLDHSEMGGGHVRTSTIGRYRQWMTHKLAT
                     WIDQFGTSGCVGCGRCITWCPVGIDITEEVAAIRASEEASG"
BASE COUNT         1166 a         2882 c         2971 g         1170 t
ORIGIN      
        1 cgcggggacg aggatccgag cctcgagccc cagccaccgc gccatccggg cgacggcccg
       61 cccgtggttg ccgtcggtgg ccgtggccag ggtcaggggc ttgaggggct cgaaccgctc
      121 cgcgagctcc tcgatcgtct cccactccgg ctcctcgccg agccgacgaa ccagcgcccg
      181 gtacgcggcc cacgaggctc cgaggatctt gaacgcgggg aggccgaggc ggctcgactc
      241 gtccttgacc cagacctccc ccacgcccag ccgctcggcg atcgagggcg cctctcggag
      301 cggggagggc tcgtacccgg ggagcttccg gtggaacggt cgcgggtcgg gctccgggcc
      361 ggggtcgagc tcctcccgga gggggttctt caggaaggag gccatcagac ctcgagcggc
      421 acgagctcga agctcaccgt gtcgatgagc ccccgatccg tgatcttcag ctcggggatg
      481 accgagagcg cgaggaagga catcgccatg aagggggagg cgagggtcgc gccgaggtcg
      541 tgggccgcgc gctccaggcg ctcgaacgcc gcggcgacct cctcggcccg gaggtccgag
      601 agcagccccc cgatcgggca cggcacctcg gccacgacct cgccttcggc cacggcgacc
      661 tgcccccctc cgatctcggc gagccggttc accgccgacg ccatgtcggc gtcgtccacg
      721 cccacgacga ccacgttgtg ggcgtcgtgg gcgtgggtcg aggcgagcgc cccacgctgc
      781 aggccgaagc cccgcacgaa gccaaggccg atccggcccg tctccctgtg ccgctccacg
      841 acggccacct tggccaggtc ccgatcgacg tcggacacgg cgtggccatc ctcggtcctc
      901 ggatccacga cgagcgcctc cgtgacgatc gacccgtcgc ggaccccgat ggcccggacg
      961 cggccgtccg cgctcacgcg gaagtcggcg gccgtgaggg tcgggacgcg gacgctgccc
     1021 cgcatccagg cgggcgcctc gatctcagcg atgtccacgg cctcgccgtc ctcggccgcg
     1081 aggcgccctc gcttccacac cttgaccgga cggaacgcct cgaggtcctc cacggccacg
     1141 acgtcggcca cgtagccggg cgccacagcc ccgtgctcgc gcaggcggtg gtaccgggcg
     1201 gcgttgaggg tccccatgac gaccgcgtct gccgggcgga tccccagcgc caccgccttc
     1261 cgcatcacgt cgttgatgtg gccctcgcgc agcagctggt ccggctcgcg gtcgtcggtg
     1321 cagaggcagc agttcgtcgg gccgtgctcc aggaccatcg gggccagccc ctcgaggttc
     1381 cgcgccgccg agccctcgcg gatcaggatc cacatcccca gccggcgctt ctccatcgct
     1441 tcgtccaggg tcgtggcctc gtggtcggag ccgacgcccg ccgcgaggta cgcgttcagg
     1501 tcccggccgg acagcccggg ggcgtgaccg tccacgtgcc cggagccggc cgcctcgatc
     1561 ttggccagcg cctcgggcgc tccggccacc accgaggggt agtccatcat ctcggccacg
     1621 ccgatcgcgc tcggctcctc ggccaggaag cgggcgatgt cctcggccgt gaccgtggcc
     1681 ccgcccgact cgaaggcgct ggccggcacg cacgaggaca ccatcacgta gaagtcgagg
     1741 ggcaccgctc cggcggaggc caagatggcc cggaccccct tcagcccgaa cacgttggcg
     1801 atctcgtgag ggtcgaggat caccgtggtc gtcccccacg gcagggccgc ccgggcgaac
     1861 tggtccacca tcagcttcgt ggactcgagg tgcatgtggg cgtcgatgaa gccggggagg
     1921 aggatcaggc ccgagcagtc ctcgacctcg gcggcatccg agtagtctcc gacccccgcg
     1981 acgtgctccc cggcgaccgc cacgttcgcc tccagcagct ccccggtgaa gacggagagg
     2041 acccggccgc ccttgagcag gaggtccgcc ggctcgtcgc cgcgcgccac ggcgaggcgt
     2101 cgagcgaggc ggtcggtgaa ggccgtcacg atggcggaca gggtagccgc gcggcgacga
     2161 gccggcaacg gcccagacct ctacccgcgg acgtgatcga acggtatcgt gtcgcgcagt
     2221 gtcgtcggtt tcgggaggtc cacaatgact cgacacgccc tccgcctctt gctcctcggc
     2281 gcgatgtgca gctccttcct cgtccccgca ggcccggcgg ccgccggcac gttccgggtg
     2341 acgatcctcg gcgatccctc accgggcgtc tgcgacgcga ggtgctcgct ccgcgaggct
     2401 gtgatcgcgg ccaacgcgtc tcccgggccc gacgtcatcc gcctgccgaa ggggcgcgtc
     2461 gagctgtcga gggctggagc aggggaggag gccggcgcca ccggcgatct ggacgtcacc
     2521 gacaccctga cgatcgttgg cgcgggcatg gggcggagcg tcgtggacgg gaacgacctc
     2581 gaccgtgtct tcgacgcgtc ctccggcacc ttcgtgctgc gccgcctgac ggtgaccggg
     2641 ggccacgtcg agacgggagg cgcgaccgcg ttcgggggcg gcatccgcgg ctggggagca
     2701 ctcgtaaggc tccgggaggt cgcggtgcga ggcaacgacg cggccgacgg gggcggtggc
     2761 gtcgcggggt tcacggtcga gatccagcga agcctgatcg ccggcaaccg agccgactcc
     2821 gccggggccg tcgacgggtc gaccgtgaag atcgcggatt ccctcctcct ggggaacagg
     2881 gccaccttgt ccgggggagc gatggcgctg ttcttcgggt ccaccgtcga gctccgcaac
     2941 accacggtcg ccgggaacac gagcgggcac aacgcggggg gcatcggcat caactccggg
     3001 agcgctctgc tgctcaacgt caccgtccac ggcaaccaca ccgacggcga cgccggggct
     3061 ccgggcggga acggaggcgg cgtccaggtc aacgcagggt caaccatgga gatccggaac
     3121 acgatcatct ccgggaacac agacggcgct cccaccgcgg cccacgactg cgccggcgac
     3181 ctgacgtccc tcgggtcgag cctcgttcgc gaccagaacg gctgcagcct caccggcgcc
     3241 tcgggtgacc tccctgcggg gacggacccg cgcctgcgcc cgctggcgaa caacggcggc
     3301 gcgaccagaa cgctggcgct cccccccggg agccctgccc ggaacgccgg agccgcctgt
     3361 ctctcgaaag accagcgtgg ggttcctcgc tcgttgggag gcgcgtgcga catcggcgca
     3421 tacgagctcg tgtcgtgctt cgggcggatc gtgaacagag tggggacgca gggcaacgac
     3481 gtcctgcgag ggaccggcag gaaggatggg ttcctcggct tcggcggccg ggatcggctg
     3541 atcggcaggg ggggctcgga cgggatgtgc ggcaacggcg gccgggacac cctcgagggg
     3601 ggaccgggca gggatcgact ccatgggggc ccgggaaggg atctctgcgt cggcggcccc
     3661 ggccgcgatc gagcccgatc gtgcgagcgc cgtcgcgaga tcccgtgacg ccgcattgcc
     3721 cgtcgggcga cgcgggtgtt gactgaagcg tcgtcgtccg aggagggcga gggtggccac
     3781 cgaggatcgc aaggacatcg cagcgctgct cgaggagcat cgcctgatcg aggccccccg
     3841 gggcttccgc gagcaggccg tggtctcgga cgaagggatc tacggccgcg cggccgagga
     3901 ccccgagggg ttctgggcgg agcaggcggg ccatctcgac tggatcgaac cgtgggacga
     3961 ggtcctgcgc tgggatccgc cctgggtgcg ttggttcgag ggtggacgcc tcaacatctc
     4021 cgcgaactgc ctcgaccgcc acctggaggc ccacggggac cgggtcgcct accactggaa
     4081 aggggagccc ggggacgagc gcacgatcac ctaccgcgag ctccacgagg aggtctctcg
     4141 cttcgcgaac gccctcaagc gtctgggcgt ggccaaggga gaccgggtcg cgatctacct
     4201 cggcatgatc cccgagctcc ccgtcgccat gctggcctgc gcccggatcg gggccgtgca
     4261 ctcggtggtc ttcgggggct tcagcgccga ctcgctgcgg gaccggatca acgacgccca
     4321 ggcctcggtg ctggtgacgg ccgacggcgg gtggcgacgg ggccagatcg tgccgctcaa
     4381 ggagaacgcc gaccaggccg tcgccgaaac tccctcgatc gagcaggtcg tcgtcgtccg
     4441 ccgcaccggc gaggaggtgc cgttccggga ggggcgcgac ctctggtacg acgagctgat
     4501 cgcggcggag agccccgact gccccgccga gccgatggag gccgaggatc ccctctacat
     4561 cctctacacg tcgggcacca ccgggaagcc caaggggatc cagcacacga ccggcggcta
     4621 cctcgtcggc gtgaccgcca cgcatcgcat gatcttcgac ctccacccgg agaccgacgt
     4681 ctactggtgc gcggccgaca tcggatgggt caccgggcac tcctacatcg tctacgggcc
     4741 gctcgccaac ggcacgacga gcgtcatgta cgagggggcg ccggacttcc ccgacaagga
     4801 ccggctgtgg tcgatcgtgg ccaagcacgg ggtgacgatc ttctacacgg cgcccacggc
     4861 gatccgtgcc ttcatgaagt gggggccgga gcacgtgcgg aagcacgacc tgtcgtcgct
     4921 ccgtctgctg ggcaccgtgg gcgagccgat caaccccgag gcctggatgt ggtactggga
     4981 gcacgtcggc ggcgagcgct gccccgtcgt cgacacctgg tggcagaccg agacggggat
     5041 gatcctcatc acgcccctgc cggggatcac ccctctgaag ccgggctcgg ccacgcgggc
     5101 cttccccggg gtcgaggccg acgtcttcga cgaggccgga ggcagcgtcc cgcccggggc
     5161 gggaggctac ctcgtcctga agcgaccgtg gccggcgatg gccaggacga tccacggcga
     5221 ccccgaccgc tacgtggagc agtacttctc gaagtacggg ccctccgtgt acctggccgg
     5281 cgacggggcg aagcgcgacg aggacggcta cttctggctg ctggggcgga tcgacgacgt
     5341 catgaacgtg gccggccacc gcctctccac ctacgagatc gagtcggcgc tcgtggacca
     5401 cccctcggtg gccgagtcgg cggtcgtctc ccgacccgac cccgacgtgg gcgaggcgat
     5461 cgtggcctac gtcacgctga agggtcacat cgagggagac gagtccctga tcgacgagct
     5521 gcgcgagcac gtggcggaga agatcgggaa gatcgctcgc ccccacgcga tcgtgctcac
     5581 cgacgacctc cccaagaccc ggtcggggaa gatcatgcgg cgtctcctcc gcgacgtcgc
     5641 ccacggccga gacctgggcg atgtgacgac cctggccaac gccgaggtcg tcgaggagat
     5701 ccgcgagctg gcaacgtcga gcccacccga agagtgacgg aagcccccgg tcggcgtcgg
     5761 gccctcgcgc tgctcaccct ggccatggtg ctcgggctcg ccacgtggtt ctcggcctcg
     5821 gcggtgatcc cccagctccg ggaggaatgg tcgctggggc cgacggcggc cgcctggctc
     5881 acgatcgccg tgcagctcgg cttcgtcgcc ggcgcgctcg tgtcggcggt cctgaacctg
     5941 gccgacgtcc actcggcgag gaccgtgatc gtcgtctccg gcttcggtgc ggcgctggcg
     6001 aacctggggc tcgtcgcggc ggacgacgcc gcgctgggga tccccctgcg gttcctgacc
     6061 gggttcttcc tggccggcgt ctacccgccc gggatgaagg tgatcgcgac ctggtaccga
     6121 gaggggcgcg gctacgcgct gggggtgctc gtcgccggca tctctctggg ctccgccatg
     6181 ccgcacctgg tcaacggtct cgggggcctc tcctggcgga ccgtgatcgt catcacctcg
     6241 atcctgacgg cctcgggagg ggtgatcgct cggtacctcg tcggggacgg ccccttcccc
     6301 ttcccccgag ccgtcttcga cccgaggcag gcggggcgcg tgttccggaa ccgaggcgtg
     6361 cggctggcga cgttcggcta cttcgggcac atgtgggagc tgttcgcgat gtgggcctgg
     6421 ttcctcgtct tcttcacgga gtccctggag accgcgggcc gcgatccgac cgttctcgct
     6481 ccgctcgcca ccttcgccgt gatcggatcc ggagccctcg ggtgctgggc ggcgggcatc
     6541 ctgggcgatc ggtggggccg ctcccgcacc acgagcgcgg cgatggcgat cagcggggcg
     6601 tgcgcgctga cgatcggcct gctcttcgga cgctccccct ggctcgtcct cgccctgggg
     6661 atcgtgtggg ggatcaccgt ggtcgccgat tcggcccagt tctcggccgc ggtcacggag
     6721 accgcgaaca ccgcctacgt gggcacggcc gtgacgctcc agacggcgct cggcttcacg
     6781 ctcacggtgg ccacgatctg gctggtgccg atcgtccgcg acgccctgtc gtggtggtgg
     6841 gccttcgcca tgctggcgct cgggccggcg ctggggatcc tggcgatgca gcggctccag
     6901 cgctcccccg aggcgttccg gatcgcgggc gggcggggct gagggagcgc gccctggtat
     6961 agcatccggg aaaggtcccg acgatgagag gtccttcgta ccgcgcgagg atcctctcct
     7021 ctcgtcacac ttcgggagtg atcccgctga cggtcagacg gctgttggac cgggagggcg
     7081 tcgacgccct catccgcgtg cttcgagagc gcggctacac ggtcgtgggc ccgcggctcc
     7141 gcgaacaggc gatcgtctac gacgagatcg gctccgtcga agacctcccc gaaggatgga
     7201 cggacgagca ggagggcggc acctatcgcc tccaccggcg cgacgacgcg gcgctgttcg
     7261 gttacgcggt cggccctcat tcctggaagc gcgagctgtt ccctcccgag acgctcctgg
     7321 cgaggatccg tcggcagaac ggagaggtcg agttcgagca gcccgagccc gacgcccccc
     7381 ggtacgcctt cctgggcgtc cgcgcgtgcg aggtggcggc gatcgagatc caggatcgcg
     7441 tgttcatggc ggagggagcc gaggacccga cctacgcggc ccgaaggcgg gacgcgctgg
     7501 tgatcgcggt gcagtgcggg tctccctcgg ggacgtgctt ctgcgtctcg atggacaccg
     7561 ggcccagggt cgaggacggg tacgacctcg cgctgaccga gatcctggag ggaggccatc
     7621 ggttcctgct cgaggtgggg tcggagcggg gcgcggagat cgcggacgcc cttccctcct
     7681 cacccgccgc ggacgaggac gtgggcgcgg ccgatgccgt cgtggagcgg gcggccgcgt
     7741 cgatgggccg gcggatggag accgacggga tcaaggagct cctgtaccgg aacctcgagc
     7801 acccccgatg ggaggacgtc gcccagcgct gcctcgcctg cacgaactgc acgatggtgt
     7861 gccccacgtg cttctgctcg acgatcgagg actccaccga tctgtcgggg gagacggcgg
     7921 agcggcacag gcggtgggac tcctgcttca cgctcgatca ctccgagatg ggagggggcc
     7981 acgttcggac ctcgacgatc ggccgctacc gccagtggat gacccacaag ctcgccacgt
     8041 ggatcgacca gttcgggacg tcgggctgcg tgggctgcgg gcgctgcatc acctggtgcc
     8101 cggtcggcat cgacatcacc gaggaggtcg ccgcgatccg cgcgagcgag gaggcgtccg
     8161 gatgaccgtg gaggggctgg atcggctcc
//
LOCUS       DASUTW010000043         8338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_273657, whole genome shotgun sequence.
ACCESSION   DASUTW010000043 DASUTW010000000
VERSION     DASUTW010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8338
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273657"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1108)
                     /locus_tag="VF058_01520"
     CDS             complement(137..1108)
                     /locus_tag="VF058_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020113535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6207015.1"
                     /translation="MSDEGAASRPEPGDTRILVVDDDPDIARFVEVNLRGDGYAVEIA
                     SNGDQGLEKALANPPDLALVDVMMPGMDGFQLVERLRTDPRTAGMSIIMLTAKSLTAD
                     KVLGLTTGADDYIIKPFDPVELMARVKGTLRRAEEMRASSPLTGLPGNVRIQDEIARA
                     VASGRPFALVYADLDNFKAYNDYYGFLRGDAAIKTAARIFQDETRGVVGESAFVGHMG
                     GDDFVLLVPPEKVEDLCHAIIERFDGAMTELYDDEDRERGYIEIRSRRGDLQRFPLVS
                     LSLGVATTERRSFTHREEAVAIATEMKNYAKRSEGSSWAIDRRSAGS"
     gene            complement(1105..1773)
                     /locus_tag="VF058_01525"
     CDS             complement(1105..1773)
                     /locus_tag="VF058_01525"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX6207016.1"
                     /translation="MSQIRVAASILAADFARLGEQVKAVEPYAEIIHVDSMDAHFVPP
                     LTIGPVVIESIRPLTERLLHAHMMVARPERLLEDYATAGTDIATFHLEAASDPGAVVD
                     EADTLGLRAGIGVNPETDIEEVYPFLERLDNVIVMTLERTGYAGQPFQREQLRKVEAV
                     RKEIDRLGLAVDVVVDGGIDAETGRLCVEAGATVLAAASSIFRAEDPAAAARGLAEVA
                     GAGG"
     gene            complement(1786..3051)
                     /locus_tag="VF058_01530"
     CDS             complement(1786..3051)
                     /locus_tag="VF058_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX6207017.1"
                     /translation="MAIEAVRRVADEGAYSNRLIPSLIGRSNLGPEDRGLASELAYGT
                     LRGLLLLDAALAPLLDRPLRRAPSGARAALRVGAHQLLYTRIPPHAAVGETVAAAPPR
                     ARGFVNAVLRRLADEGWRPPEGADDGSISLRTGVAPWVVAELRALVGEEAEAAAAGIA
                     SSAGLTLRANPCAGPVEDLPGILAGAGHEVETGSIHARSLRLPGGDPRTLPGWEDGLF
                     TVQDEASAWVVDVLDPRRGERVLDCTAGPGGKATDIACRAGTVVASDLSERRAGLVAS
                     SSARLGVDVHPLVMDATRPAVRPGFDRVLVDAPCSGLGSARRRPELLWRPKEEDLAAL
                     HRLQVDLVSSAAGLLRPGGLLVYSVCTFPRAETDEVVAEILDRFPGLRPDPFATPEGE
                     RRASARLWPHRHGTDAMFAARFVLGGSRP"
     gene            complement(3113..4030)
                     /gene="fmt"
                     /locus_tag="VF058_01535"
     CDS             complement(3113..4030)
                     /gene="fmt"
                     /locus_tag="VF058_01535"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX6207018.1"
                     /translation="MGRRVVFLGNDPWSVPSLEALASSEHEVALVLTREPRPAGRGGR
                     LRPTAVAEAARRLGLPLREVATVKEGEGRAALEEAVPDVLVVVAYGEILPADVLAVPG
                     LVPVNVHFSLLPELRGADPVRRAILEGLETTGVTTMRMDEGMDTGPVLLQAEEPIRLD
                     DDAGSLGERLAALGGGLLVETLDRLEEIEERAQEHDRATTARKIRLEEEWIDWSQPAG
                     AIVRRVRALSPEPGARTRLRGKVLKVYRARSVGGAGEPGTILVSGEMVVAAGEGAVAL
                     DEVLPEGKRRMSGAELARGHRPESGERLG"
     gene            complement(4031..4555)
                     /gene="def"
                     /locus_tag="VF058_01540"
     CDS             complement(4031..4555)
                     /gene="def"
                     /locus_tag="VF058_01540"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014351715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX6207019.1"
                     /translation="MAILPIRTLGDPVLRQPASPVEEFDGALRALADDMIETMHEAPG
                     VGLAAPQVGRAIRLIVFDIGEGPSALVNPTLSGHEGEQIGEEGCLSIPGLYFEVRRAM
                     KVTADGMDLHGEPVRIEGEELLARVLQHEVDHIDGILFIDRLDEEERKRAMAELRRQE
                     LGLAPTSLDPSRAL"
     gene            complement(4569..6368)
                     /locus_tag="VF058_01545"
     CDS             complement(4569..6368)
                     /locus_tag="VF058_01545"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207020.1"
                     /translation="MGRAVGVAVDLPRLELDRPFTYLLPEGMEAPVGSVVSVPFHGRT
                     VHGWVLGPTDDVPSRVLPVRRVLSKEPVFDQRALRLFAWMRERYVVPLSVAIDRAVPP
                     RVASEEGGAAGSPPESRAAVPGALGRYESGGALLTACREGSGVFVVRPLPEDEAAVCV
                     DAVTATVAGGRDAVVVIPELDPLPEIAAAVLEAAGPAGALFAGGDRRARYRTWLEILR
                     GRYRVVVGTRPAIFSLLPRVGLLWIHREAHAVHREERTPAYHPREVALARAELEGAVC
                     ALAALAPSGHAAAMVEDGEAVLVRAPRAIERAAAPLVETTRPADEDRSPRLATLLRSA
                     PGAFLLLSRRGYGTARVCRACGEPARCAACRGPIVVRKGREACAVCGAEARCAACGAA
                     DFGVERGGTERIEEWARGVTSLPVRRVDEGAASGPEPGEVVVGTAAAVKDLGPRSIGL
                     VGILDADRARRRAGLEAPEQVLATWFEAAAWAGPRAEGGRVLVQTREPADPAVQALVR
                     WDPLHFHRRERARREEAGFAPGVPLFRVVGSPELEEALRALEPASLLATPLGGQTVCL
                     VTIRPDALAAFRERILDLVGEHVALRVEAEPRL"
     gene            complement(6373..7557)
                     /gene="metK"
                     /locus_tag="VF058_01550"
     CDS             complement(6373..7557)
                     /gene="metK"
                     /locus_tag="VF058_01550"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017576778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX6207021.1"
                     /translation="MARTHLFTSESVTEGHPDKLADQISDAVLDAILTDDPGARVACE
                     TLVTSGLAIIAGEISTRTYVDLRDVVRETITEVGYTDPALGIDGEACGVITAIQEQAG
                     DIAQGLEDSWEARRGSDDELDRLGAGDQGMMIGYACTETAELMPAPIAIAHRLTKRLA
                     DVRKAGVVPYLRPDGKSQVTVEYEDDRPRRITTVVVSAQHREEVDVETLLTPDVVEHV
                     IEPVLAEVGLDADDVTHMVNPTGKFEKGGPAADTGLTGRKIIVDSYGGLVPHGGGCFS
                     GKDPTKVDRSGAYFVRYVAKNIVAAGLADRAQIQVAYAIGTAHPVSFVLETFGTSEVD
                     PERLETTVRELFDFRPAAIIRDLDLRRPIYRETAAYGHFGRKDVPWEATEKADELRRA
                     FA"
     gene            complement(7576..>8338)
                     /gene="coaBC"
                     /locus_tag="VF058_01555"
     CDS             complement(7576..>8338)
                     /gene="coaBC"
                     /locus_tag="VF058_01555"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX6207022.1"
                     /translation="GVTIVGPVEGPLAAGDEGMGRMAEPEEIAGALEEALGGGDLAGR
                     RILVTAGPTYEPVDAVRFVGNRSSGRMGFAVAEEARRRGAEVILVTGPTHLSDPPAIE
                     VRRVETAAQMRQEVLLAFEDADAVVKAAAVSDYRPAEPSARKLKKEEGPPPIRLEPTA
                     DILAELGERKGERVLVGFAAETDDLEAAGRRKLTEKRLDLVVVNEVGRPGTGFEAETN
                     RAMILSARGDDEPLRDWTKRDLARAIADRLAKLLT"
BASE COUNT         1067 a         3243 c         2948 g         1080 t
ORIGIN      
        1 ggactgtcac cgtcggtccc gggattccgc cgggtccacc cccgcgtggc tcgcgggggg
       61 tcgtggactg tcaccaccgg tggggagttc caccccgccc cgaaggctcg gttgcccgac
      121 cagtatagaa cggcgctcag ctgccggcgg accgtcggtc gatggcccag gaggagccct
      181 cggaccgctt cgcgtagttc ttcatctcgg tggcgatggc cactgcttcc tcgcggtgcg
      241 taaacgatcg gcgctcggtg gtggccaccc ccagggagag cgagacgagc gggaagcgct
      301 gcaggtcccc tcgtcggctc cggatctcga tgtagccgcg ctcccgatcc tcgtcgtcgt
      361 agagctcggt catcgccccg tcgaaccgct cgatgatggc gtggcagagg tcctcgacct
      421 tctcgggggg gaccagcagc acgaagtcat ccccgcccat gtggcccacg aaggccgact
      481 cgccgaccac gcccctcgtc tcgtcctgga agatcctggc cgccgtcttg atcgcggcat
      541 ccccccgcag gaacccgtag tagtcgttgt aggccttgaa gttgtcgagg tccgcgtaga
      601 cgagggcgaa cgggcggccc gaggccacgg cccgggcgat ctcgtcctgg atcctgacgt
      661 tgcccggaag gcccgtgagg ggcgaggagg cccgcatctc ctcggcccgc cgcagggtgc
      721 ccttgacccg cgccatcagc tcgaccgggt cgaacggctt gatgatgtag tcgtcggccc
      781 cggtcgtcag ccccagcacc ttgtcggcgg tcagcgactt cgccgtcagc atgatgatgc
      841 tcatcccggc cgtgcgcgga tcggtccgga ggcgctctac gagctgaaac ccgtccatac
      901 ccggcatcat cacgtccacc agggccaggt cgggcgggtt ggccagggcc ttctccagcc
      961 cttggtcgcc gttcgacgcg atctccacgg cgtagccgtc gccccggagg ttgacttcga
     1021 cgaatcgggc gatgtcggga tcgtcgtcca ccacgaggat cctggtgtcg ccgggctcgg
     1081 gccgggaggc cgcgccctcg tcgctcatcc gccggccccc gcgacctcgg ccaggccgcg
     1141 cgcggccgcc gccgggtcct cggcccggaa gatggaggag gcagctgcca ggaccgtggc
     1201 gcccgcctcc acgcacagac gcccggtctc cgcgtcgatg ccgccgtcga ccaccacgtc
     1261 caccgcgagc cccaggcggt cgatctcctt ccgcaccgcc tccaccttcc ggagctgctc
     1321 ccgctggaac ggctgaccgg cgtacccggt gcgctccagc gtcatcacga tcacgttgtc
     1381 gagccgctcc aggaacgggt agacctcctc gatgtcggtc tcggggttca ctccgatgcc
     1441 cgcccggagg cccagggtgt cggcctcgtc gaccaccgcg cccgggtccg acgccgcctc
     1501 gaggtggaag gtggcgatgt cggtgccggc cgtggcgtag tcctcgagga gccgctcggg
     1561 ccgcgccacc atcatgtgcg cgtggagcag ccgctcggtc aggggccgga tcgactcgat
     1621 cacgacgggc ccgatcgtga ggggcgggac gaagtgcgcg tccatggagt ccacatggat
     1681 gatctcggcg tagggctcga ccgccttgac ctgctccccg aggcgggcga agtccgcggc
     1741 caggatcgag gcggccaccc tgatctgcga catggcgccc cgattctacg gccgggaccc
     1801 acccagcacg aagcgggcgg cgaacatcgc gtcggtgccg tggcggtgtg gccagaggcg
     1861 ggccgacgcc cgacgctccc cctccggggt cgcgaacggg tcggggcgca gacccgggaa
     1921 ccggtccagg atctccgcca cgacctcgtc cgtctcggcg cgagggaagg tgcagaccga
     1981 gtacacgagc aggccgcccg gtcgcaggag gccggccgcg ctcgagacga ggtcgacctg
     2041 gaggcggtgc agcgcggcga ggtcttcctc cttgggccgc cagaggagct cggggcggcg
     2101 gcgagccgag cccaggcccg agcacggggc atcgaccagc acgcggtcga agccggggcg
     2161 cacggcgggc cgggtcgcgt ccatgacgag cgggtggacg tccaccccca gccgggcgga
     2221 cgaggaggcc accaggcccg cgcgacgctc cgagaggtcc gaggcgacga cggtccccgc
     2281 ccggcacgcg atgtcggtgg ccttgccccc cgggccggcc gtgcagtcca gcacccgctc
     2341 cccccggcga gggtccagca cgtccaccac ccatgcggag gcctcgtcct ggacggtgaa
     2401 gaggccgtcc tcccacccgg ggagggtcct gggatcgccg cccgggaggc ggagcgaccg
     2461 ggcgtggatg ctccccgtct cgacctcgtg gccggcgccg gccaggatgc cggggaggtc
     2521 ctcgaccggg cccgcgcacg ggttggcccg aagggtgagg cccgcggagg aggcgatccc
     2581 ggccgccgcc gcctcggcct cctcgcccac gagggcgcgg agctcggcca cgacccaggg
     2641 cgcgaccccg gtacgcagcg agatcgagcc gtcgtcggct ccttcgggag ggcgccagcc
     2701 ctcgtcggcc aggcgccgga gcacggcgtt cacgaatccc cgagcccggg gcggcgccgc
     2761 ggcgaccgtc tcgcccacgg cggcgtgcgg ggggatgcgg gtgtacagga gctgatgggc
     2821 cccgacgcgg agggcggcgc gagcgccgct cggcgcgcgc cgcagcggtc ggtccaggag
     2881 cggcgcgagc gccgcatcga ggaggaggag cccccgcagg gtgccgtagg cgagctccga
     2941 cgccagcccc cggtcctcgg gaccgaggtt cgagcgtccg atcagggagg ggatgaggcg
     3001 gttgctgtat gcgccctcgt cggcgacgcg gcgcacggcc tcgatcgcca ccgcccgtgg
     3061 cgtccgcagc gggggcgcgg cccggccccg aggccgtccg ccgcgccgcc ccctacccga
     3121 gccgctcgcc cgactccggc cggtggccac gggccagctc cgctccgctc atccggcgct
     3181 tcccctccgg gagcacctcg tccagcgcga cggcgccttc gcccgccgcg acgaccatct
     3241 cccccgagac gaggatcgtc cccggctcgc cggcgccccc caccgaccgc gcccggtaca
     3301 ccttcaggac ctttccccgc aggcgcgtcc tggccccggg ctcgggcgag agcgcccgca
     3361 cgcggcggac gatcgccccc gcgggctggg accagtcgat ccactcctcc tccaggcgga
     3421 tcttccgcgc ggtcgttgcc cgatcgtgct cctgggcccg ctcctcgatc tcctccagcc
     3481 gatcgagcgt ctccaccagc aggcccccgc ccagcgcggc gaggcgctcg ccgagcgagc
     3541 ccgcgtcgtc gtccagacgg atcggctcct ccgcctgcag gagaacgggg ccggtgtcca
     3601 tcccctcgtc catccgcatg gtcgtcacgc cggtcgtctc caggccctcc aggatcgccc
     3661 tccggaccgg gtcggcgccc cggagctcgg ggagaagcga gaagtgcacg ttgaccggga
     3721 ccagccccgg aacggcgagc acgtcggccg gcaggatctc accgtaggcc acgacgacga
     3781 ggacgtcggg aacggcctcc tccagggccg ctcggccctc gccttccttg acggtggcca
     3841 cctcccggag cggcagcccc agccggcgcg cggcctcggc caccgccgtg ggccggagcc
     3901 gccccccgcg cccggctggc cgcggctcgc gggtcaggac gagggcgacc tcgtgctccg
     3961 aggaggccag ggcctccagc gaggggaccg accagggatc gttcccgagg aagacgacgc
     4021 ggcgtcccac tcagagcgcg cgcgaggggt ccagcgaggt gggcgccagc cccagctcct
     4081 gccggcggag ctcggccatc gcgcgcttcc gctcctcctc gtccagccgg tcgatgaaca
     4141 ggatgccgtc gatgtgatcc acctcgtgct ggaggacgcg ggccagcagc tcctcgccct
     4201 cgatgcgcac cggctctccg tggaggtcca tgccgtccgc cgtcaccttc atcgcccggc
     4261 gaacctcgaa gtagagtccg gggatcgaga ggcagccctc ctcgccgatc tgctccccct
     4321 cgtggcccga gagcgtggga ttgacgagcg cgctcggccc ctcaccgatg tcgaagacga
     4381 tgagccggat cgccctcccc acctgggggg cggccagccc cacgccgggg gcctcgtgca
     4441 tcgtctcgat catgtcgtcg gccagcgccc ggagggcccc gtcgaactcc tccaccgggg
     4501 acgccggctg gcgcagcacc gggtcgccca gcgtccggat cggaaggatc gccatctcgc
     4561 actcctcgtc acagtcgcgg ctcggcctcg acccggaggg cgacgtgctc gccgacgagg
     4621 tcgaggatgc gctcccggaa cgccgcgagg gcgtcggggc ggatcgtcac caagcatacc
     4681 gtctggcccc ccagcggcgt cgcgagcagg ctcgcgggct cgagcgcccg gagcgcctcc
     4741 tccagctccg gcgagcccac gacccggaag agggggacgc cgggcgcgaa ccccgcctcc
     4801 tcgcggcggg cccgctcgcg gcggtggaag tggagcggat cccagcgcac gagcgcctgc
     4861 accgcggggt cggccggctc ccgcgtctgg acgaggaccc tccccccctc ggcgcgaggg
     4921 ccggcccagg ccgcggcctc gaaccacgtg gccaggacct gctcgggcgc ctccagcccg
     4981 gcccggcgcc gggcgcgatc cgcgtctagg atgcccacca gcccgatcga cctcggcccc
     5041 aggtccttca ccgcggccgc ggtgcccacg acgacctccc ccggctcggg ccccgacgcg
     5101 gcgccctcgt ccacccggcg gacggggagg ctcgtcacgc cgcgggccca ctcctcgatc
     5161 cgctcggtcc cgccccgctc caccccgaag tctgcggccc cgcaggcggc gcagcgggcc
     5221 tccgcgccgc agacggcgca cgcctcgcgc cccttccgca cgacgatcgg cccccggcag
     5281 gcggcgcagc gggccggctc gccgcaggct cggcagacgc gcgccgtccc gtagccgcgc
     5341 cgcgacagca ggaggaacgc gcccggcgcc gaccgcagca gggtcgccag cctgggcgag
     5401 cggtcctcgt cggccggcct cgtggtctcc acgagggggg ccgccgcgcg ctcgatcgcc
     5461 cggggggccc ggaccagcac cgcctcaccg tcctcgacca tggccgcggc gtgcccgctg
     5521 ggcgcgaggg cggccagggc gcagaccgcc ccctcgagct ccgctcgcgc cagcgccacc
     5581 tccctcggat ggtaggcggg cgtccgctcc tcgcggtgga cggcgtgcgc ctcccgatgg
     5641 atccagagaa ggccgaccct tgggagcagc gagaagatgg ccggcctcgt ccccaccacg
     5701 acccggtagc ggccccgcag gatctccagc caggtccggt agcgggcccg ccggtcccct
     5761 cccgcgaaga gggcgccggc cggcccggcc gcctccagca ccgccgccgc gatctcgggc
     5821 agcgggtcga gctcggggat caccaccacg gcatctcgcc cgccggccac cgtggccgtc
     5881 acggcgtcca cgcagacggc cgcctcgtcc tcggggagcg gccggacgac gaacaccccg
     5941 ctcccctctc ggcacgccgt cagcagcgct cctccgctct cgtaccggcc cagggctccc
     6001 gggacggcgg cccgggactc agggggggac cccgccgctc ctccctcctc ggacgccacc
     6061 cggggcggga ccgcccgatc gatcgccacc gacagcggga ccacgtagcg ctcccgcatc
     6121 caggcgaaga gccgcagcgc tcgctggtcg aagacgggct ccttcgacag gacccggcgc
     6181 acgggcagca cccgcgacgg gacgtcgtcg gtagggccca gcacccatcc gtggacggtg
     6241 cggccgtgga acgggacgct gacgaccgag cccaccggcg cctccatgcc ttcagggagg
     6301 aggtacgtga acggccggtc gagctccagg cgggggaggt ccaccgccac cccgacggcc
     6361 cgccccacgg cctcaggcga acgcgcgccg gagctcgtcg gccttctcgg tggcctccca
     6421 ggggacgtcc ttgcgcccga agtggccgta ggcggcggtc tcccggtaga tcgggcgccg
     6481 caggtcgagg tcgcggatga tcgccgccgg ccggaagtcg aacagttcgc gcacggtggt
     6541 ctcgagcctc tccggatcca cctccgacgt cccgaaggtc tccagcacga acgagacggg
     6601 gtgggcggtg ccgatcgcgt acgcgacctg gatctgggcc cggtcggcga gtcccgcggc
     6661 cacgatgttc ttcgccacgt agcgcacgaa gtaggcgccg gaccggtcca ccttcgtggg
     6721 gtccttcccc gagaagcagc cgccgccgtg ggggacgagc cccccgtagg agtccacgat
     6781 gatcttccgg ccggtgaggc ccgtgtcggc cgccggcccg cccttctcga acttgcccgt
     6841 cgggttcacc atgtgcgtga cgtcgtcggc gtcgagcccc acctccgcga ggaccggctc
     6901 gatcacgtgc tccacgacgt cgggcgtgag gagggtctcg acgtcgacct cctcgcggtg
     6961 ctgggcggag accaccaccg tcgtgatccg gcgggggcgg tcgtcctcgt actccaccgt
     7021 gacctggctc ttgccgtctg ggcggaggta cggcacgacg ccggccttgc ggacgtcggc
     7081 cagccgcttc gtcaggcggt gggcgatcgc gatcggggcg ggcatcagct cggcggtctc
     7141 ggtgcaggcg tacccgatca tcatgccctg gtcgccggcc ccgagccggt cgagctcgtc
     7201 gtccgagccg cggcgcgcct cccaggagtc ctccaggccc tgggcgatgt cccccgcctg
     7261 ttcctggatc gcggtgatga cgccgcaggc ctccccgtcg atccccaggg cagggtctgt
     7321 gtacccgact tcggtgatcg tctcgcggac gacgtcgcgg aggtccacgt aggtgcgggt
     7381 cgagatctca cccgcgatga tcgccaggcc cgaggtcacg agcgtctcgc aggccacgcg
     7441 ggcgcccggg tcgtcggtga ggatcgcgtc gaggacggcg tcggaaatct ggtcggcgag
     7501 cttgtccggg tgcccctcgg tcaccgactc cgaggtgaag aggtgggtcc gcgccatgcc
     7561 cggattctat aggcctcagg tcaggagctt ggccaggcgg tcggcgatgg cccgggccag
     7621 atcccgcttc gtccagtcgc ggagcggctc gtcgtcgccc cgagccgaga ggatcatggc
     7681 gcggttcgtc tcggcctcga agcccgtgcc cgggcgaccc acctcgttca ccaccacgag
     7741 gtcgagacgc ttctcggtca gcttccgccg gccggcggcc tcgaggtcgt cggtctcggc
     7801 ggcgaagccg accagcacgc gctcgccctt ccgctccccc agctcggcga ggatgtcggc
     7861 cgtgggctcc agccggatcg ggggcggccc ctcctccttc ttcagcttgc gggcggaggg
     7921 ctcggccggg cggtagtcgc tcacggccgc cgccttcacc accgcatccg cgtcctcgaa
     7981 ggcgagcagg acctcctggc gcatctgggc cgccgtctcc accctgcgca cctcgatcgc
     8041 cggggggtcg gagaggtgcg tggggcccgt gacgaggatc acctccgcgc cccggcgccg
     8101 ggcctcctcg gccaccgcga agcccatccg gccgctcgat cggttcccga cgaagcgcac
     8161 ggcatccacc ggctcgtagg tggggccggc cgtcacaagg atccgcctcc ccgccaggtc
     8221 cccgcccccg agcgcttcct ccagcgcgcc cgcgatctcc tcgggctcgg ccatccgccc
     8281 catgccctcg tcccccgcgg ccagcggtcc ctccacgggg cccacgatcg tcacgccc
//
LOCUS       DASUTW010000044         8004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1439899, whole genome shotgun sequence.
ACCESSION   DASUTW010000044 DASUTW010000000
VERSION     DASUTW010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8004
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439899"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VF058_01560"
     CDS             <1..889
                     /locus_tag="VF058_01560"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6207023.1"
                     /translation="PARPPRPVMTLFLQQLFAGLAQGSVYALVAFGYALIFSTSRVVN
                     FAQGHLFMLGAMTGVALLGVGVPYLLMVPLTMAIVAASGLALERSITLPLRATRSPYT
                     WIVATLAAAVVIENVASMIFGKEPRTFPPAIAGVTRIGDLVIQHHALVVLAVAVAVMV
                     GIELWLGRTMIGTAFRATADNPAPARLSGVPVPRLVMLSFALSGAIAGLGGVLVAPIT
                     FAAVPIGLLLGLKGFIAAVIGGMGSFRGALIGGLSLGLAETVIRQQLPAGMGGTILFG
                     FLLLTLVLRPTGLFGRRVA"
     gene            886..2676
                     /locus_tag="VF058_01565"
     CDS             886..2676
                     /locus_tag="VF058_01565"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEX6207024.1"
                     /translation="MRARRASFWGGVALVLVGASLVYLPVGDFYLFLASRSACFLLVV
                     AGLNLALGYTGLFSFAHVALFALGAYATAVAVTRQGLPVWAGIGSALLVGAVAGAVLT
                     LATFRARAIHFAVVSLVLVFAVSELLSGWTSVTGGEAGITVRRPELAGDALMARRYWL
                     FVLAIAAVCLLAIRNLVRSPLGRALVAVRESEEAAASVGIDPFRYRTLAMAFGGAVAA
                     AGGALFAHLDGFVSPEISGFDDATLFVAMLLVGGAGALWGPVLGVGAFLLIDRALVSL
                     QGALPGLDLVVWQALVSGILLFLVIVFLPRGMAGALTSRRRGATVADRDETPAAAVLP
                     APRIPWPEGPALEVVGLTKRFGGVRAVDDLDLVVEGGTIHGLIGPNGSGKTTTVNLLT
                     GALAADAGEIRFLGKRVVRPRPHAMAARGVGRVFQRTEVFAAIASLENVMAGFHLVAE
                     RGLPANVLRTPGARRRDEELRREAATLLEALGLAGRRHLPAASLPFGDRRLLEVARAL
                     AARPTVLILDEPATGLSTGELGRLAALLARLREGGVTVLLIEHNMEFLMGLCDRVTVL
                     DSGRRIAEGTPTEVQSDPKVVEAYLGVEAG"
     gene            2673..3401
                     /locus_tag="VF058_01570"
     CDS             2673..3401
                     /locus_tag="VF058_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019010685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6207025.1"
                     /translation="MTASPLLRIRGLACSYGAITALREVDLDAHPNEVTCVLGVNGAG
                     KSTLLAALAGAIRPSAGSVRVGGVETVGTTPERMVGLGVVLVPEGRQVFPSLTVRDNL
                     LLGAYRLRRDRSAVSEGLDEVHRLFPVLAELAERPAGALSGGEQQMLAIGRALMGRPR
                     ILMLDEPSLGLAPRVVGVVMEAVAGLAAGGRSVLLVEQLARVALQASHRGYLLESGRV
                     VLEGTSEDLQADDRVREIYMGVKG"
     gene            3398..5035
                     /locus_tag="VF058_01575"
     CDS             3398..5035
                     /locus_tag="VF058_01575"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6207026.1"
                     /translation="MRTTEPLAPEVEATDRSRAGPWIRLTAAVLGLAVIGVAARMLAD
                     FQWDRVVEYRSPYVFDLPPAPETPRLTDRVILVVVDGLRVDASEGMPTLQRLGAEGSS
                     LVARTPLPSLSFPGWTNLVSGAPPDVSSVTTNWYEGRVRVDSVLASARRAGLTTAVAG
                     GEGWRQLFRGQIDHSFYVPDARRFADERIGRAALRMLAEDDPDLLIVHLPDVDHRGHE
                     SGVDGAYRDAALGADRAIEALAEAAEEDTAIIVAADHGHIESGGHGGPEEEVVRTPLV
                     LWGDGLVPGAQGAVAQSDIAPTIAALLGIGRPAHATGELRVELLDADDEARAEIESAH
                     DETLARFYEAATGALEASGSTEAAFERARQDRHRHDVLARLPIALGVAAVMAIAVALA
                     TRGLDAVAILVGSIGFFGTWAGIFLARGLTFSFSHFNTEDQVETFLVARLLDTLIALG
                     VASVVAGVVAGRRQRAGGFETGLGMTAWILLITGMAVAGFLVVFGWGFVWGLPSLVAG
                     FAEFLALLAMFGVGVAAWAAGLVSLGTAAVVAPRRAR"
     gene            5070..5543
                     /locus_tag="VF058_01580"
     CDS             5070..5543
                     /locus_tag="VF058_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007529264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061136 - regulation of proteasomal protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome activator"
                     /protein_id="HEX6207027.1"
                     /translation="MDEEQRTPDVIVPGMRKDEDDSQEIVEHPAKLLRIASMIRELLE
                     EARHSSLDEGGRRRLQEIYDRALVELKDVLTEDLRRELDALAIPFTDTPSESEIRVAQ
                     AQLVGWLEGLFHGIQAALWAQQMQARQQLEEMRRRGLPSGLPGQQQGQTPPGQYL"
     gene            5545..5913
                     /locus_tag="VF058_01585"
     CDS             5545..5913
                     /locus_tag="VF058_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207028.1"
                     /translation="MDELHAFVGFAIVGGFALLTLWGLATWLIRRGPGKAFWWVVGTL
                     QAALIVQLLVGIVLLVMGGGRGTLHYLYGVGFPALILVGAHWLAREAFADRPWAPFAV
                     GSFFIFGLTLRALMTGLGIG"
     gene            6026..6259
                     /gene="infA"
                     /locus_tag="VF058_01590"
     CDS             6026..6259
                     /gene="infA"
                     /locus_tag="VF058_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX6207029.1"
                     /translation="MERAGPQKEDALQVEGTIVEALPNVMFRIDLDNGHNILGHISGK
                     MRKHYIRILPGDRVTVELSPYDLTRGRIIYRHR"
     gene            6279..7478
                     /locus_tag="VF058_01595"
     CDS             6279..7478
                     /locus_tag="VF058_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX6207030.1"
                     /translation="MIPRRTLAVGAAVALLLLWWASGLDRSPVPADRFFSQAEIARAG
                     AYRTPLYAATALRLTLDLGLLAVLAFTPAGAWLGRLRGSRWIRGAALAIGAIVLLRFL
                     VRLPISFWAGYVHEHSWGFSTQTVWGWLGDAGRSVGLALAVSTPAYVGAVWAARRWPR
                     RWPALLVPATAALILLGSFVWPVVVEPLFNRFEPLRRPAAERFEALAHRAGVPVDAVL
                     VADASRRTRKENAYVSGFGGTKRLVLYDTLLRSVSPEEAEVVVAHELGHRRRGHVLRG
                     TLVGAVAGAGAILLIALLARTRWLRRAGGATGPDDPRMLPALLLGISLLSLAALPPSN
                     LLSRSFEEEADRIALALTRDRQAYEAAHRGLALRNLSDLDPGPVAFRFLATHPAAPER
                     IAYALEP"
     gene            complement(7460..>8004)
                     /locus_tag="VF058_01600"
     CDS             complement(7460..>8004)
                     /locus_tag="VF058_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX6207031.1"
                     /translation="AGRAFAGGYCYLNSAAAAAAALHEEAGPVAILDLDYHHGNGTQE
                     IFRDDPTVLYVSIHADPDVEYPYFTGRGDDEGQATVNLPLPLGTRDDAYLEVAERAFA
                     RTADHAPEALVVSLGLDAHEDDPMGRFALTDEAFGALGSMTAALGPPVLIVQEGGYLS
                     GSLGGAVRAFLDAVDHGSSA"
BASE COUNT          903 a         2795 c         3041 g         1265 t
ORIGIN      
        1 cccggcgcgc ccccctcgac ctgtcatgac cctgttcctc cagcagctgt tcgccggcct
       61 ggcccagggg agcgtctacg ctctcgtggc cttcgggtat gccctgatct tctccacgtc
      121 gagggtagtg aacttcgccc agggccacct gttcatgctc ggcgcgatga ccggcgtcgc
      181 cctgctgggg gtcggcgtcc cctacctcct gatggtgccc ctgaccatgg cgatcgtggc
      241 ggcctccggc ctcgcccttg agcgctccat cacgctgccg ctgagggcga cccgcagccc
      301 gtatacgtgg atcgtggcca cgctggccgc cgccgtggtg atcgagaacg tcgcgtccat
      361 gatcttcggg aaggagcccc ggaccttccc gcccgcgatc gcgggggtga ccaggatcgg
      421 ggatctcgtg atccagcatc acgccctcgt cgtgctggcc gtcgccgtgg ccgtgatggt
      481 cgggatcgag ctgtggctcg ggaggacgat gatcgggacg gccttccggg ccacggccga
      541 caacccggcc cccgcccggc tctcgggcgt gccggtgcct cgcctggtga tgctctcgtt
      601 cgcgctctcc ggggcgatcg cgggcctggg cggcgtcctg gtggcgccga tcacgttcgc
      661 ggccgtcccg atcgggctgc tgctcggcct caaggggttc atcgccgcgg tgatcggcgg
      721 gatggggtcc ttccgagggg ccctgatcgg cgggctgtcg ctcgggctgg ccgagacggt
      781 gatccgccag cagctccccg cggggatggg cggcacgatc ctgttcggct tcctgctcct
      841 cacgctcgtc ctgcggccga ccgggctctt cgggagacgg gtggcgtgag ggcccgccgg
      901 gcgagcttct ggggaggggt tgccctggtg ctcgtcggcg cctcgctcgt gtacctgccc
      961 gtcggcgact tctacctgtt cctggcctcc cggagcgcct gcttcctgct cgtcgtggcg
     1021 gggctgaacc ttgccctcgg gtacacgggc ctgttctcgt tcgcccacgt cgcgctgttc
     1081 gcgctgggag cctacgcgac ggcggtcgcg gtcacgcggc agggcctccc cgtgtgggcc
     1141 gggatcggct cggcgctgct ggtcggggcg gtggccgggg ccgtgctcac cctggccacg
     1201 ttccgggccc gggcgatcca tttcgccgtg gtctcgctcg tcctcgtctt cgccgtctcc
     1261 gagctcctga gcggctggac ctcggtgacg gggggcgagg cgggcatcac cgtgcggcgg
     1321 cccgagctcg ccggggatgc cctcatggcc cggcggtact ggctcttcgt cctggcgatc
     1381 gccgcggtct gcctcctggc gatccggaac ctcgtgcgct cgcctctggg gcgggccctg
     1441 gtggcggtgc gcgagagcga ggaggcggct gcgtcggtgg ggatcgaccc gttccggtac
     1501 cggaccctgg ccatggcctt cgggggagcg gtggccgcgg ccgggggggc gctcttcgcc
     1561 cacctggacg gcttcgtcag cccggagatc tcggggttcg acgacgccac gctcttcgtg
     1621 gcgatgctgc tggtgggcgg cgccggagcg ctctggggcc ccgtgctggg agtgggggcg
     1681 ttcctcctga tcgatcgggc cctcgtgtcc ctgcagggag ccctcccggg actcgacctg
     1741 gtcgtctggc aggcgctggt ctcgggcatc ctcctcttcc tcgtgatcgt cttcctgccc
     1801 cggggtatgg cgggcgctct gacctcccgc cgtcgtgggg cgacggtcgc cgatcgggac
     1861 gagacgccgg ctgcggcggt gcttcccgcc ccgcggatcc cgtggccgga gggcccggcg
     1921 ctcgaggtgg tgggcctgac gaagcgcttc ggcggcgtcc gggcggtgga cgacctcgat
     1981 ctcgtggtgg agggcggcac gatccacggg ctgatcggcc ccaacgggtc gggtaagacc
     2041 acgacggtga acctgctcac cggcgccctt gcggccgacg ccggcgagat ccggttcctg
     2101 gggaagcgag tggttcggcc ccggccccac gccatggccg cccgcggggt ggggagggtg
     2161 ttccagcgga cggaggtctt cgccgcgatc gcctcgctgg agaacgtcat ggcggggttc
     2221 catctcgtgg ccgagcgcgg cctccccgcg aacgtgctcc ggaccccggg ggcccgccgc
     2281 cgggacgagg agctgcggcg agaggcggcg accctcctcg aggcgctcgg gctcgccggg
     2341 cgccggcatc tgccggccgc atcgcttccg ttcggcgacc gtcggctgct cgaggttgct
     2401 cgcgcgctgg ccgctcgtcc gaccgtgctg atcctggacg agccggccac cggtctctcc
     2461 acgggggagc tgggccggct cgcggcgctg ctggctcgac tgcgggaagg gggagtcacg
     2521 gtcctgctga tcgagcacaa catggagttc ctgatggggc tctgtgaccg ggtcacggtg
     2581 ctcgactcgg gtcgcaggat cgccgagggt acgccgacgg aggtccagtc cgaccccaag
     2641 gtggtcgagg cctaccttgg ggtggaggcg ggatgacggc gagcccgctg ctgcggatcc
     2701 ggggcctggc ctgctcgtac ggggcgatca ccgccctccg cgaggtggac ctcgacgcgc
     2761 acccgaacga ggtcacctgc gtgctcggcg tcaacggggc ggggaagtcc acgctgctgg
     2821 cggcgctggc cggagcgatc cgcccgtccg cgggcagcgt gcgcgtgggc ggcgtggaga
     2881 ccgtggggac cacgccggag cgcatggtgg ggctgggggt cgtgctcgtc cccgagggac
     2941 ggcaggtgtt cccctcgctc accgtccgcg acaacctgct cctcggcgcg tatcgtctcc
     3001 ggcgggaccg gtcggcggtc agcgaggggc tcgacgaggt gcatcgcctc ttccccgtcc
     3061 tcgctgagct ggcggagcgg cccgcggggg ccctctcggg gggcgagcag cagatgctcg
     3121 cgatcgggag ggcgctcatg gggaggcccc gcatcctcat gctggacgag ccctcgctcg
     3181 gtctggcgcc ccgcgtcgtg ggcgtcgtga tggaggccgt ggcggggctc gccgcggggg
     3241 gccgctcggt gctgctcgtg gagcagctcg cccgcgtggc gctgcaggcg tcgcaccgag
     3301 gctacctgct ggagtcgggg agggtggtcc tggaagggac gagcgaggac ctgcaggccg
     3361 acgaccgggt ccgcgagatc tacatgggag tgaagggatg aggacgacag aaccgctggc
     3421 gcccgaggtg gaggcgaccg accggtcccg ggccggaccg tggatccggc tgaccgccgc
     3481 ggtgctgggg ctggccgtga tcggcgtcgc cgcccggatg ctggccgact tccagtggga
     3541 ccgggtggtc gagtaccgct cgccgtacgt cttcgatctc cccccggccc cggagacgcc
     3601 tcggctcacc gaccgcgtga tcctggtcgt ggtggacggc ctacgcgtgg acgcctccga
     3661 gggcatgccg accctccagc ggctgggcgc ggagggctcc tcgctcgtgg cgcgaacgcc
     3721 cctgccctcg ctgtcgttcc cggggtggac gaacctggtc tcgggcgccc ccccggacgt
     3781 ctcgagtgtg accacgaact ggtacgaggg gcgcgtgcgt gtcgactcgg tgctcgcctc
     3841 ggcccggcgc gcgggcctca cgaccgccgt ggccggcggg gagggctggc gccagctctt
     3901 ccgagggcag atcgaccaca gcttctacgt cccggacgcc cggaggttcg ccgacgagcg
     3961 catcgggcgg gccgcgctcc gcatgctggc cgaggacgac cccgatctcc tgatcgttca
     4021 cctccccgac gtggatcacc gggggcatga atcgggcgtg gacggagcct accgggacgc
     4081 ggccctcgga gccgatcgag cgatcgaggc gctggccgag gcggccgagg aggacacggc
     4141 gatcatcgtc gctgcggacc acggccacat cgagagcggc gggcacggtg gccccgagga
     4201 ggaggtcgtc cggacgccgc tcgtcctgtg gggagacgga ctcgtccccg gagctcaggg
     4261 cgccgtcgcg cagtcggaca tcgcgcccac gatcgccgcg ctgctgggga tcgggcggcc
     4321 ggcgcacgcc acgggcgagc tgcgcgtcga gctcctcgac gcggacgacg aggccagggc
     4381 cgagatcgag tcggcgcacg acgagacgct ggcgcgcttc tacgaggcgg ccacgggggc
     4441 gctcgaggcc tccgggtcca cggaggcggc gttcgagcgc gccagacagg accgccatcg
     4501 ccacgatgtg ctggcccggc tccccatcgc gctcggcgtg gcggccgtga tggcgatcgc
     4561 ggtggccctg gccacccggg gcctcgacgc cgtggcgatc ctcgtcggct cgatcgggtt
     4621 cttcgggacg tgggccggca tcttcctggc ccgcggcctc accttctcgt tctcgcactt
     4681 caacaccgag gaccaggtgg agaccttcct cgtggcccgg ctcctggaca cgctcatcgc
     4741 gctcggggtg gcctcggtcg tcgcgggggt cgtcgccggg cggcggcagc gggcgggcgg
     4801 gttcgagacc ggcctcggca tgaccgcctg gatcctgctg atcacgggca tggcggtggc
     4861 gggtttcctg gtcgtgttcg gctgggggtt cgtctggggg ctcccgagcc tcgtggccgg
     4921 cttcgcggag ttcctggccc tgctcgcgat gttcggcgtg ggcgtggcgg cttgggcggc
     4981 cggtctcgtc tcgctgggga cggccgcggt cgtggcgccc cggcgagcgc gctgaggcgc
     5041 gcgctccctc cgcgtgtagg ctggtagcca tggacgagga acagcggacc ccggacgtca
     5101 tcgtgccggg tatgaggaag gacgaggacg actcccagga gatcgtcgaa caccccgcga
     5161 agctgctccg gatcgcctcg atgatccggg agctgctgga ggaggcccgg cactcgtctc
     5221 tggacgaggg aggccgcagg cggctccagg agatctacga tcgggcgctc gtcgagctga
     5281 aggacgtcct caccgaggac ctgcgccggg agctcgacgc gctggcgatc ccgttcaccg
     5341 acacccccag cgagtcggag atccgcgtgg cccaggccca gctcgtgggc tggctcgagg
     5401 gcctcttcca cgggatccag gcggccctct gggcccagca gatgcaggcc cgccagcagc
     5461 tcgaggagat gcggcggcgg ggccttccca gcgggctccc cggccagcag caggggcaga
     5521 ccccgccggg ccagtacctc tgagttggac gagctgcatg ccttcgtggg cttcgccatc
     5581 gtgggcggct tcgccctgct cacgctgtgg ggcctcgcca cctggctgat ccggagaggg
     5641 cccggcaagg ccttctggtg ggttgtcggc acgctgcagg cggccctgat cgtccagctc
     5701 ctcgtgggga tcgtgctgct ggtgatggga gggggacggg ggaccctcca ctacctgtac
     5761 ggcgtggggt ttcccgcgct gatcctcgtg ggggcgcact ggctggcccg cgaagccttc
     5821 gccgatcggc cctgggcgcc gttcgcggtg ggatccttct tcatcttcgg gctgacgctg
     5881 cgggcgctca tgaccgggct cggcatcggc tgatcgggtc acctgctacc ctggccgcat
     5941 ggcacgaagg aagggaagaa ggccgcctcc ccgccgatcg gcgggcagac ggcagccgct
     6001 cacggccgag cagcgggcca agcggataga acgggccggc ccccagaagg aggacgccct
     6061 ccaggtcgag gggacgatcg tcgaggcgct gccgaacgtg atgttccgga tcgacctcga
     6121 caacgggcac aacatcctcg ggcacatctc ggggaagatg cgcaagcact acatccgcat
     6181 cctcccgggc gaccgcgtga cggtggagct ctcgccctac gacctcacgc ggggccgcat
     6241 catctaccgg caccgctgat cccccggggg catccccggt gatccctcgt cgcacgctgg
     6301 ccgtcggagc ggcggtcgcg ctcctcctgc tgtggtgggc ctcggggctc gatcgctccc
     6361 ccgtccctgc cgaccgcttc ttctcccagg ccgagatcgc tcgggccggc gcgtaccgga
     6421 cccccctgta cgcggcgacg gcgctgcgcc tcaccctcga cctcgggctg ctggccgtgc
     6481 tggccttcac gcccgccggg gcctggctcg ggcgcctgcg ggggtcgcga tggatccgcg
     6541 gcgcggcgct cgcgatcggg gcgatcgtcc tgctgcgctt cctggtccgg ctcccgatct
     6601 ccttctgggc cggctacgtc cacgagcact cgtgggggtt ctccacccag acggtgtggg
     6661 ggtggctggg cgacgcggga aggtcggtcg ggctggcgct ggcggtctcg acccccgcgt
     6721 acgtgggcgc cgtgtgggcg gctcggaggt ggcctcggcg gtggccggcg ctcctcgtgc
     6781 cggccaccgc cgcgctcatc ctcctgggct cgttcgtctg gccggtcgtc gtcgagccgc
     6841 tcttcaaccg gttcgagcct ctgcgccggc cggcggccga acgcttcgag gccctggctc
     6901 accgggcggg ggtccccgtg gacgccgtgc tcgtggcgga cgccagccgg cgaacgagaa
     6961 aggagaacgc ctacgtctcg ggcttcgggg gcacgaagcg cctcgtactc tacgacacgc
     7021 tcctgcgatc cgtgtccccg gaggaggccg aggtcgtggt ggcccacgag ctggggcatc
     7081 ggcgacgagg ccacgtgctt cgaggcaccc tcgtgggggc ggtcgccggc gcgggcgcga
     7141 tcctgctgat cgctctcctg gcgaggaccc ggtggctgcg gagggccggt ggggcgaccg
     7201 gcccggacga cccgcggatg cttccggcgc tgctgctcgg catctcgctg ctcagcctcg
     7261 cggcgctccc gccctccaac ctcctctcgc gctcgttcga ggaggaggcg gatcggatcg
     7321 ccctggccct cacgagggac cggcaggcct acgaggccgc ccaccggggc ctggcgcttc
     7381 ggaacctctc cgacctcgac ccgggaccgg tcgccttccg attcctggcc acccaccccg
     7441 cggcgcccga acggatcgcc tacgcgctcg agccgtgatc gacggcgtcg aggaaggcgc
     7501 ggaccgcgcc gccgagcgac ccggagaggt atccgccctc ctgcacgatg aggacggggg
     7561 gtccgagagc ggccgtcatc gaccccagcg ctccgaacgc ctcgtcggtg agggcgaacc
     7621 gccccatcgg atcgtcctcg tgagcgtcga ggcccagcga gacgacgaga gcttccggcg
     7681 cgtggtcggc ggtgcgggcg aaggcgcgct ccgcgacctc caggtacgcg tcgtcgcggg
     7741 ttccgagggg gagggggagg ttcacggtgg cctggccctc gtcatccccc cgaccggtga
     7801 agtaggggta ctcgacgtcg gggtcggcgt ggatcgagac gtagagcacc gtcggatcgt
     7861 ctcgaaagat ctcctgggtc ccgttcccgt ggtggtagtc gagatcgagg atcgccacgg
     7921 gaccggcttc ctcatggagg gcggccgcgg cggcggcggc gctgttgagg tagcagtagc
     7981 cgccggcgaa ggcccggccg gcgt
//
LOCUS       DASUTW010000045        11396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1698875, whole genome shotgun sequence.
ACCESSION   DASUTW010000045 DASUTW010000000
VERSION     DASUTW010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11396
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698875"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1661
                     /locus_tag="VF058_01605"
     CDS             87..1661
                     /locus_tag="VF058_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6207032.1"
                     /translation="MPGSGPRPAPRRILSLALTALAVACTQPPPEARESPIPGPTLSP
                     QPTLRVGVGPIATLDPARVETPDEILAAAQIFDGLVEVDASGEIVPAAAASWEIEEGG
                     ARFVFRLEGSTFHDGSPVRAQDFVFAWERLVDPVERYPFAFLLEEVEGFGEHRASFGA
                     RPLTGIEARDAATLAVTLSEPNMGFLALLSHPALSPIREGSSAPSLAALPIGNGPYAL
                     AEDLGLDGPIVLEAAGGVEVGVARVELRPSEEVEEAWPEFLARRLDVARIPAAFVGDE
                     DEEGVRATGRLLYCGFNLRSRRFSRAVRVAVSIAIDREALARGVYGGLATRADGLVPP
                     TFPGYRAGACDERCELDRLRAEELIAELPRRERAFRLDFPTSEVGIGVAQGVQRQLAQ
                     IGLNVNLRGHDEDQYARLLQEGEQEAFCLVWVADAPTAQAVLDPLLDPDSPDNRTGIE
                     DEDLARLLERARTAASTEEREELYARAERRALELMPLVPLAWFRSNVAAQEYVTGLEV
                     DPLGRFDMAALAFDEG"
     gene            1694..1780
                     /locus_tag="VF058_01610"
     tRNA            1694..1780
                     /locus_tag="VF058_01610"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1728..1730,aa:Leu,seq:gag)
     gene            complement(1926..2372)
                     /locus_tag="VF058_01615"
     CDS             complement(1926..2372)
                     /locus_tag="VF058_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207033.1"
                     /translation="MRRFVSLLPYLPVLAAIGWLATRFRVILGLFLIAHGLVHVLYVV
                     PEPAQKAGGLEWPFHLDRSWALPGLGEGTVRAIGLLLMIATIAGFALAGIAVLANLGW
                     WTGASIAAAAASTALLGLYFHPLLVLGLAINVFVLSVAGFRWPALS"
     gene            2579..3292
                     /locus_tag="VF058_01620"
     CDS             2579..3292
                     /locus_tag="VF058_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207034.1"
                     /translation="MSVEVTQRGLAIPELQEPSAGRKLGAGSATRVVVATFGVLVALA
                     GIEHGVGEILQGSVRPEGLVIESWADSEAFEILSGEPAMTVIPNLLWAGILTIAAALA
                     VGVWSVGFAHRRRGGLVLIGLSAVLLLAGGGFGPPLIGIIVGVAATRIGSIPARRPSR
                     VARALGRAWAWVLGTAVLGYLSLVPGVVILDQALRVESASLVAGLTALSFAALLLALV
                     AARAHDRARAGQAGSPAGR"
     gene            3375..3536
                     /locus_tag="VF058_01625"
     CDS             3375..3536
                     /locus_tag="VF058_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207035.1"
                     /translation="MYRYLRRNPGWRELDPREKERNKRHLHGFTRIFPHGRTKVFRYR
                     PPYTTKARS"
     gene            3632..4069
                     /locus_tag="VF058_01630"
     CDS             3632..4069
                     /locus_tag="VF058_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6207036.1"
                     /translation="MKTRMVHIEGEILVDRPVEEVFDFVADERNRYDPKVREAEKLTP
                     GPIGVGTRFRSLATSMGRTVEMTIEITAHDRPYRLGSSTHLSSMDIHSTLSFERVPGG
                     TRMRWSSDPEPRGLLRLAGPLVGWMGRRQAREIWAQLKRVLEG"
     gene            4078..4788
                     /locus_tag="VF058_01635"
     CDS             4078..4788
                     /locus_tag="VF058_01635"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6207037.1"
                     /translation="MVTITRPGGEMTAYVATPAAGGQWPGVVVIHDALGMSTDLRNQA
                     DWLAREGYLAVAPDLFHWGSRMRCLFSTMRAAVAGEGRPFEDIEAARAWVAGRGDCTG
                     RVGVIGFCLGGGFALMLASLADYGASSVNYGGVPKDAMAALEGACPVVGSYGGRDRSL
                     RKAPGQLERALTELGIEHDIKVYPEAGHAFLNDPARGEMPLWAKVAGSFANAGYHEPS
                     AADARRRIVSFFDAHLKS"
     gene            4815..5099
                     /locus_tag="VF058_01640"
                     /pseudo
     CDS             4815..5099
                     /locus_tag="VF058_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010838310.1"
                     /note="involved in translesion DNA polymerization with
                     beta clamp of polymerase III; belongs to Y family of
                     polymerases; does not contain proofreading function;
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
     gene            6004..7500
                     /locus_tag="VF058_01645"
     CDS             6004..7500
                     /locus_tag="VF058_01645"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX6207038.1"
                     /translation="MASVLLSEERTLPIQEPVRGRFAYLEHPGVLALAGFEQIQRFRR
                     GELPYGPLWYLTGLDLVEFGVGTATFRLPATGWLHSPAGLLTGGALAFAADGALGSAI
                     LSTLPPRRVLATSDLSLNFLRPPAEGSAILARARVIQVGRFQALSEAAVEDDTGRLLA
                     HATSRCVITEVPGPLPEPPEGAIPWPDHPRPHPFERPPEGEVVPQEMWDRMEGVDMQR
                     AWQRGELARPPLSNLLGTEARRVEEGAIALAMPASPWFANTGGAFYGGSAALFADYAI
                     HGAIHSSAAAGGSWATLDLKVRFLRPIIPDGRPLEARARVVHWGRRVAVSSAEILTTE
                     GKVAALADSSVMLLPGRPWRVPAAPIDEGPPEDAAEERAMAGRRAAGWAFGVTAVALF
                     MTALDSLVVVTALPVITPPPGSDARRCVRPTRDRVRGQRPDGQVIGWQRTESRENRCG
                     LWLASAPTPQTSAAQYPYSEEVGWVSLFHGGCPAAHLLTSHPPRPRRG"
     gene            8395..9942
                     /gene="otsB"
                     /locus_tag="VF058_01650"
     CDS             8395..9942
                     /gene="otsB"
                     /locus_tag="VF058_01650"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX6207039.1"
                     /translation="MGVLARISAAAIDAVIFDLDGVVTDTASVHSAAWKRLFDAYLQE
                     RAERTGDDWEPFEPDDYRRYVDGKPRYDGVRSFLAARGITLPEGETSDPPDRETVRGL
                     GNRKDREFLDHIREHGVDAFPGTVRLIDDLHGLGVRTAIISASRNCQEVLRAAGLEDR
                     FEERVDGLVAAGVGLPGKPDPAVFLEAARRLGVPPFRAASVEDALAGVEAGRRGGFAL
                     VIGVDRTGHAQELREHGADVVVSDLGEMSTTAEPPAIGDLPDALDRVARVATWVDERS
                     PAVFLDYDGTLTPIVPDPDDALLSDEARAAIRRLAEVCTVGVISGRDLDDVRGKVALD
                     ELLYAGSHGFDIVGPDGLREQRGTEHLPALDEAEADLRAALENTGTAVERKRFAVAVH
                     FRRAPDRAAEVERAVKAAASAHRGLRVTGGKMIFELRPDVEWDKGLALSWLLEVLGLE
                     GEDVLPMYLGDDVTDEDAFRALGARGVGLVVRGEDDTRPTLASLAFSDPGEVRSFLDA
                     LAEVLEE"
     gene            9939..>11396
                     /locus_tag="VF058_01655"
     CDS             9939..>11396
                     /locus_tag="VF058_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEX6207040.1"
                     /translation="MRLWTLSYEGFVPEQEALREALCVLGNGYFATRGAAPESSADDI
                     HYPGTYLAGGYGRLETEIAGRVVENEDLVNLPNWLVLSFRVEGGEWFDLRGVEILDYV
                     QDLDLRQGVLVRRLRFRDAEGRVTDLSQRRLVHMEDAHLAALQTTFVAENWSGRIEVR
                     SGIDGTVTNSGVARYRDLRGDHLVPQGTGSIGEDGIWLVVETRTGKLRVAEAVRTRVL
                     RDGTPADVPRRLVEREGFVAHDLDVDLPRGEPVTVDKVVALATGRGRPGYEPVIDATT
                     YVQRAGTFEDLLPSHVLAWDLLWGRFDLSLNGPERAQLVLRVHIFHLLQTASEHTIDL
                     DVGVPARGLHGEAYRGHIFWDELFIFPFLNYRLPALTRSFLEYRHRRLPEARAAARAA
                     GYDGAMYPWQSGSTGREESQTLHLNPESGRWLPDNSHLQKHINLAVPYNVWQHYQVTG
                     DLEFLRFHGADMLIEIARFWSSIATYNRSLDRYEIL"
BASE COUNT         1600 a         3924 c         4176 g         1696 t
ORIGIN      
        1 gctctctggg ggcacctcgg tcgagcgaac cctcgaccgc atcaccgtca tcacggcgct
       61 ggtcttcgcc ggcaccacgt tctggcttgc ctggcagtgg gcctaggccc gccccacgcc
      121 gcatcctctc gctggcgctg accgcgctgg cggtggcctg cacccagccg ccccccgagg
      181 cccgcgagtc gccgatcccg ggccccaccc tgagcccaca gcccacgctc cgcgtcgggg
      241 tcggtccgat cgcgacgctc gaccccgctc gcgtcgagac ccccgacgag atcctggcgg
      301 ccgcccagat cttcgacggg ctggtcgagg tcgacgcctc gggcgagatc gtcccggcgg
      361 cagccgcctc gtgggagatc gaggaggggg gagcgaggtt cgtgttccgg ctcgaaggct
      421 cgaccttcca cgacggctcg ccggtccggg cgcaggactt cgtcttcgcg tgggagcgcc
      481 tcgtggatcc ggtcgagcgc tatccgttcg cgttcctgct ggaggaggtg gaggggttcg
      541 gggagcaccg ggcgagcttc ggggctcggc ccctgaccgg gatcgaggcc cgggacgccg
      601 ccacgctggc ggtgacgctc agcgagccga acatgggctt cctcgccctg ctctcgcatc
      661 cggcgctgtc cccgatccgg gaggggagct cggcgccctc gctggccgcc ctgcccatcg
      721 ggaacgggcc gtacgcgctg gcggaggatc tcgggctcga cggccccata gtcctggagg
      781 ccgccggggg ggtcgaggtg ggcgtggccc gcgtggagct tcgtccctcg gaggaggtcg
      841 aggaggcctg gcccgagttc ctggcccgcc ggctcgacgt ggctcggatc cccgccgcgt
      901 tcgtggggga cgaggacgag gagggggtcc gagccaccgg caggctcctc tactgcgggt
      961 tcaacctgcg gtcccggcgg ttctcccggg cggtgcgcgt cgctgtgtcc atcgcgatcg
     1021 accgagaggc cctcgcccgg ggcgtgtacg gggggctggc cacacgggcg gacggcctgg
     1081 tgccgcccac cttccccggc tacagggccg gggcgtgcga cgagcgctgc gagctggatc
     1141 gcctccgcgc cgaggagctg atcgcggagc tcccccgccg cgagcgggcc ttccggctcg
     1201 acttccccac gtcggaggtg gggatcggcg tggcccaggg ggtccagcgc cagctggcgc
     1261 agatcggcct gaacgtgaac ctccggggcc acgacgagga ccagtacgcc aggctgctcc
     1321 aggaggggga gcaggaggcc ttctgcctgg tgtgggtggc ggacgcgccg accgcgcagg
     1381 cggtcctcga tccgctcctc gatcccgact ccccggacaa ccggacgggg atcgaggacg
     1441 aggacctcgc gcggctgctg gagcgggccc ggaccgccgc atcgaccgag gagcgcgagg
     1501 agctgtacgc gcgcgcggag cgccgcgccc tcgagctgat gccgctcgtg ccgctggcct
     1561 ggttccgatc gaacgtggcg gcccaggagt acgttaccgg cctcgaggtg gatccgctgg
     1621 ggcgcttcga catggccgcc ctggcgttcg acgagggctg agggcccggc ccgtcgctat
     1681 aatcgggacc gtcgtcggag tggcggaact ggcagacgcg ctaggttgag ggcctagtgg
     1741 ggctaaccac cccgtgggag ttcgactctc ccctccgaca cttctgacga gcagaaacgc
     1801 tccccgactt ctcgaacact cgttccgtgg gcgtcccctg gtcacacttt tggtcacagt
     1861 cggcaccgag ccgctgagca cggtgcggtc gcgctcttac ccacagccca tctcaggcct
     1921 cggccttagc tcagcgcggg ccaccggaag cccgcgaccg acaggacgaa cacattaatg
     1981 gccagcccga ggacgagcag ggggtggaag tacaggccca gcagcgcggt ggacgccgcg
     2041 gccgcggcga tagaggcacc ggtccaccag cccaggttcg ctagcacggc gatcccggcg
     2101 agggcgaacc cggcgatcgt ggcgatcatg agcaagagcc cgatcgcccg aaccgtgccc
     2161 tcgccgaggc cgggcagggc ccaggaccga tcgaggtgga agggccactc gaggccgccc
     2221 gccttctgcg ctggctcggg aacgacgtag aggacgtgga ccagcccgtg ggcaatcagg
     2281 aacagcccca ggatgacccg gaaccgggtc gcgagccacc cgatggcggc gagcacgggg
     2341 aggtagggga gcagggatac gaagcgtcgc atgtcggatc actcctctct gctcacgcta
     2401 cgaggaggcc ggccgcgtgt cgggggcccc atggcccggt tctcagggga cgatggtcag
     2461 cccgaggtgg tgtccgaagg tcccgccgat cgcccgacgt gagaccctga ccggcctccg
     2521 gtgtgcggcg tttcatggtg agcgcgaggt tcatggcgcc ggaaggaggg gcggaccgat
     2581 gtcagtcgag gtgacacagc gcggtctagc gatcccggag ctgcaggagc cgtccgcggg
     2641 ccggaagctg ggggccggga gcgcaacgag ggtcgtcgtc gccaccttcg gggtgctggt
     2701 ggcgctggcc ggcatcgagc acggggtcgg ggagatcctc caagggtcgg tccgccccga
     2761 ggggctggtc atcgagtcgt gggcggactc ggaggccttc gagatcctga gcggggagcc
     2821 ggccatgacg gtcatcccga acctgctctg ggcgggcatc ctgacgatcg ccgccgccct
     2881 cgccgtcggc gtgtggtcgg tggggttcgc ccaccggcgc cgcggcgggc tggtgctgat
     2941 cggtctgtcg gcggtcctgc tcctcgcggg gggcgggttc ggcccgccgc tgatcgggat
     3001 catcgtcggg gtcgccgcca cgaggatcgg gtccatcccc gcccggcggc cgagcagggt
     3061 cgcccgggcc ttgggtcggg cctgggcgtg ggtcctgggg actgcggtcc tcggctacct
     3121 gtcgctggtg ccaggcgtgg ttatcctcga tcaggccctg cgcgtggaga gcgccagctt
     3181 ggtggcgggg ctcacggccc tctccttcgc cgccctcctc ctggcccttg tcgccgcccg
     3241 ggcccacgac cgcgcgcgag ccggccaggc aggatcgccg gccgggcgct aggagctcca
     3301 tgagtgagaa ggccgccgga gaacgggggc tccaggtcga ccgcgccgtc taagccgtcg
     3361 acgaggagca gagggtgtac cggtacctcc gccggaatcc ggggtggcgg gagctcgacc
     3421 cgagggagaa ggagcggaac aagcgccatc tccacggctt cacgaggatc ttcccgcacg
     3481 gccggaccaa ggtgttccgc taccggcccc cctacacgac gaaggcccgg tcttgaccga
     3541 ggggctggcg gcccgctcga ttcgcgtgcc ggctgggaga ccctcgagta gcgggtgcct
     3601 ccctgtacgg ccagactatc gtcgaggggg agtgaaaacg cggatggtcc acatcgaggg
     3661 cgagatcctc gtcgatcgac ccgtggagga ggtattcgac ttcgtcgccg acgagcgcaa
     3721 ccgctacgac ccgaaggtcc gagaggcgga gaagctcacg ccggggccga tcggggtcgg
     3781 aacgcgcttc cggtccttgg ccaccagcat gggccggaca gtcgagatga cgatcgagat
     3841 cacggcccat gatcggccgt atcggctggg gtcatccacc cacctgtcgt ccatggacat
     3901 ccacagcacc ctgtccttcg agcgggtccc cggcgggacg cggatgcggt ggtcgtcgga
     3961 cccggagccg cgcggtctcc tcaggctggc cgggccgctc gttggctgga tgggccggcg
     4021 gcaggcccgc gagatctggg cccagctcaa gcgcgtcttg gagggatgag agatggcatg
     4081 gtgacgatca ccagaccggg tggtgagatg acggcgtacg tggctacccc cgccgccggg
     4141 gggcaatggc ccggcgtcgt cgtgatccac gacgccctgg ggatgagcac cgatctcagg
     4201 aaccaggccg actggctcgc ccgggagggc tacctcgccg tcgcccccga cctcttccac
     4261 tgggggagcc ggatgcggtg cctgttctcc acgatgcggg ctgccgtcgc cggcgaggga
     4321 cgaccgttcg aggatatcga agccgcccgc gcctgggtcg ccggccgcgg ggactgcacc
     4381 gggagggtcg gcgtgatcgg gttctgcttg ggcggaggct tcgcgctgat gctggcctcg
     4441 ttggccgact acggggcgtc gagcgtcaac tacgggggcg tcccgaagga cgccatggcg
     4501 gctctcgagg gtgcctgccc ggtggtgggg agctacgggg gaagggatcg gtcgctgcgg
     4561 aaggcccccg ggcaactgga gcgtgcgctg accgagctcg ggatcgagca cgacatcaag
     4621 gtgtatccgg aggccggcca cgccttcctc aacgacccgg cccgggggga gatgccgctg
     4681 tgggcgaagg tggcggggag cttcgccaac gccgggtacc acgagccgtc cgctgccgac
     4741 gctcgcaggc ggatcgtctc gttcttcgac gcgcacctga agtcgtagcc ccccgaagct
     4801 gcccatctcc ctcggtggtt ccaacgatcc tgcacgccga cgtcgacgcc ttctatgcgt
     4861 ccgtcgagca gcgggatgac cctcggctcc gcggccgtcc ggtcgtcgtc ggcgggggcg
     4921 tcgtgctcgc ggccagctac gaggcccagg cctacggcgt gcacactgcg atgggcggcg
     4981 cggaggcccg gcggcgctgc ccgcaggcgg tcgtcgtgca gcctcgcatg tccgcctact
     5041 ccgaggcgag caaggcgctc ttcgccgtct tcgaggacac gacgccgctg gtcgaggggc
     5101 gatgcgggtg tttgacgagg aagtgttcgg cccggtgcgg tgcgtaatgg cggtggagga
     5161 cctggaccaa gccatctcgg tcatcaatgg gagcgactac gggcacaccg ccgccatcta
     5221 cacggagtcg ggcggcgcgg cgaggtactt cacacggcac gtccagaccg gccaggtggg
     5281 ggtgaacgtc gggaccccgg ccccgatcgc cttctatccc gtcgggggtc gcaagacgtc
     5341 cttctacggg tcactccggg gcgcgccaat gacgccatcg acctctacac cgacaagaag
     5401 gtcgtcgtct tcacctggca cttcaagtga cggatgtgaa aggagagctg cccggctgct
     5461 ggccgaacat ggagggcggt caagaagggg caggtgggcg gcgggtgaca cggaggacgc
     5521 acatcgggtc tcaggttccg acggaccgcg ctagggtcgc gccctagcgg agggtggtca
     5581 cacttctggt cacgctcggg cgctttcagg gccgtccagc gcgggccggc gccgtccgcc
     5641 gaagcggcga caccctcatt gacctgcagc gtggtcgtgg acgaacgact gcgtaccgtg
     5701 aacgcggggt tgagggccta gtggggctaa ccaccccgtg ggagttcgac tctcccctcc
     5761 gacaccgctg agcaggtaaa acgctccggg gcgtcttcgc tcgcgagtca gcctggtcac
     5821 agttctggtc acactccgcc ccggatacag gcccgactac ctggggcaag cgacggcctc
     5881 tgctgggact gggccggaag ctcgccggtc cgaaggtccc aggcgggccg ggccgtcgga
     5941 ccctggctgc gcgaccggtc gtccccgagg ctgggggaca gccgaggcag cgaggtggcg
     6001 ggcgtggcct ccgtacttct gagtgaggag cggaccctcc cgatccagga acctgtccgc
     6061 gggcggttcg cgtatctcga gcatccaggc gtcctggcgc tggccgggtt cgagcagatc
     6121 cagcggttcc ggcggggaga gctcccgtac ggaccgctct ggtacctcac agggctcgac
     6181 ctagttgagt tcggggtcgg caccgcgacc ttccgcctcc ccgcgaccgg gtggctccac
     6241 agtcccgcgg gcctgctgac cggcggcgcg ctagccttcg ccgctgatgg ggccctcggg
     6301 agcgcgatcc tctccaccct cccgccccga agggtgctgg ccacgtcgga cctcagcctg
     6361 aacttcctcc ggcctcccgc cgaaggttcg gcgatcctcg ctcgggcccg ggtgatccag
     6421 gtgggccgct tccaggcgct ctccgaggcc gcggtcgagg acgacaccgg gcgcctgctg
     6481 gcccacgcca cctcccgatg cgtgatcacc gaggtgccgg ggcccctgcc ggagcctccc
     6541 gagggggcca tcccgtggcc ggaccacccg cgcccccacc ccttcgagcg cccaccggag
     6601 ggagaggtgg ttccccagga gatgtgggac cgcatggagg gggtcgacat gcagcgggcc
     6661 tggcagcggg gggagctggc ccgcccgccc ctctccaacc tgctggggac cgaggcccgc
     6721 cgggtcgagg agggggccat cgcgctggcc atgccggcca gcccgtggtt cgccaacacc
     6781 ggaggagcgt tctacggtgg ctcggcggcg ctcttcgccg actatgccat ccacggagcc
     6841 atccactcct cggccgcggc cggaggctcc tgggccacgc tcgacctgaa ggtccggttc
     6901 cttcgcccca tcatccccga cgggcgcccg ttggaggccc gggcgcgggt ggtccactgg
     6961 ggccggaggg tcgcggtttc ctccgcggag attctcacga ccgagggaaa ggtggcggcc
     7021 ctggcagatt cctcggtgat gctccttccc ggccggccct ggcgcgtccc ggccgcgccg
     7081 atcgacgagg gaccccccga ggacgccgca gaggagaggg cgatggccgg acggcgggcc
     7141 gcggggtggg ccttcggcgt caccgccgtg gccctgttca tgacagcgct cgacagcctg
     7201 gtggtggtca ccgccctgcc ggtgatcaca cctccaccag gttctgacgc tcgccgatgt
     7261 gtccgcccga cgagggatcg agttcgaggc cagcggccgg atggccaagt gatcgggtgg
     7321 cagcggaccg agtcgcggga gaatcggtgt ggtctatggc tggcgagcgc gccgaccccg
     7381 cagacgagcg ccgcccagta cccctattct gaggaagtcg gctgggtttc cctgtttcac
     7441 ggcggatgtc ccgcggcgca tctcctgacc tctcatccac ctcgtcctcg gagaggttga
     7501 agggccgggc cgggccccag tgcttcgcct cgctgctgag gatgggctgc cacaagcccg
     7561 gcccgccacc ccggcgcacg tccgccgacc gggggcccga tggcttcaga tggaacaccg
     7621 cttcgggcga gcagccagga gatcaccgag ttcgagttcc acatctcgcc cgcccggagt
     7681 tcgtcccggc cccacaccaa ggcgggcacg gaagggacca tgtccaggag accacgcgcc
     7741 aagcgctgat cgtcactcag acgctgggga ctcgcgacgg cccaggccgc gtcccgaatg
     7801 tggccgtcct gccagcggcg cagctcgtac cggaacacgc ggaagggctc aaggaggcgg
     7861 cttccgacgg ggccttcgag gaccaccccg cgcgattcgc catcggcgtc ggggatgggc
     7921 gtcatctcga ccacgaaccg tccctccggg acccggacct ccagcgccga gtgatagagg
     7981 tggagcggtc cccggcgttc gaggcgcgac ttgatggcct cgtagatccg cccgttcagc
     8041 cgaacgaacc atcccctcgc gccgagcggg agccagtaca gatcgatgcc cgttctggaa
     8101 ggcgcacgcg cgggtggtgt ctccctgcca ccaaccgggc tcggcagcgc gtgaacagcc
     8161 atgacttggt gctccggcga cctcctccgt ctcctggatc gtcacgctct cgcctcgacc
     8221 ccccgcggtg tagggtcgcc ggccccgccg cgacagggtc cttcgccctc cctccgggtg
     8281 gcgttcctgg gcatcgcgtg tcccctaggc gttccggcgc tcgcctgacc ctctccgttc
     8341 ccgcctccgg gggccaccgc gcgataggct gattcgcacg acggagaggg gttgatgggg
     8401 gttctcgcgc ggatctcggc cgcggcgatc gacgccgtga tcttcgatct cgacggggtc
     8461 gtgaccgaca cggcgtcggt ccactccgcg gcctggaagc ggctgttcga cgcctacctc
     8521 caggagcggg cggagcggac gggggacgac tgggagccct tcgagcccga cgactatcga
     8581 cgctacgtgg acggcaagcc tcggtatgac ggcgtgcggt ccttcctggc cgcgcggggc
     8641 atcacgctcc ccgagggcga gacgtccgac ccgccagacc gggagacggt tcgagggcta
     8701 ggcaaccgca aggaccggga gttcctcgac cacatccgcg aacacggtgt cgatgcgttc
     8761 cccggcaccg tccggctgat cgacgacctg cacgggctcg gggtgaggac ggcgatcatc
     8821 tcggcgagcc gcaactgcca ggaggtcctg cgagcggcgg gactcgagga ccgattcgag
     8881 gagcgggtcg acgggttggt cgccgccggg gtgggcctgc cggggaagcc cgacccggcc
     8941 gtcttcctcg aggccgcgcg gcgcctgggc gtccctccct tccgcgccgc gagcgtcgag
     9001 gacgccttgg ccggggtgga ggccgggcgg cggggagggt tcgctctcgt gatcggggtg
     9061 gaccggaccg ggcacgccca ggagctgcgg gagcacgggg ctgacgtggt cgtctccgac
     9121 ctcggggaga tgtcgacgac cgcggaaccc cccgcgatcg gcgaccttcc ggatgcgctc
     9181 gaccgggtgg cgcgcgtcgc cacctgggtt gacgagcgca gccccgccgt cttcctggac
     9241 tacgacggga cgctcacccc gatcgtgccc gatcccgacg acgccctgct ctcggacgag
     9301 gcccgcgccg ctatccgtcg gctcgccgag gtctgcacgg tcggggtgat cagcgggcgg
     9361 gatctcgacg acgtgcgcgg taaggtcgcg ctggacgagc tcctgtacgc ggggagccac
     9421 gggttcgaca tcgtcggtcc ggacgggctc cgcgagcagc gggggaccga gcacctgccg
     9481 gccctggacg aggcggaggc ggacctccga gcggccctgg agaacaccgg gaccgcggtg
     9541 gagcggaagc ggttcgcggt ggcggtccat ttccggcggg cgcccgaccg tgccgccgaa
     9601 gtcgagcgcg cggtgaaggc cgccgcgtcg gcccatcgcg ggctccgggt caccgggggg
     9661 aagatgatct tcgagcttcg gcccgacgtg gaatgggaca agggcctggc cctgtcgtgg
     9721 ctgctcgagg tgctgggtct ggagggcgag gacgtgctcc ccatgtacct tggcgacgac
     9781 gtcaccgacg aggacgcctt ccgggcgctg ggcgcgcggg gcgtgggact cgtggtccgg
     9841 ggagaggacg acacccgccc gactcttgcc tccctcgcgt tcagcgatcc gggcgaggtg
     9901 cgctccttcc tggacgccct cgcggaggtg ctcgaggagt gaggctgtgg accctctcct
     9961 acgaggggtt cgtccccgag caggaagcgc tccgcgaggc cctatgcgtg ctcgggaacg
    10021 gctacttcgc cacccgtggg gcggcgccgg agtcctccgc cgacgatatc cactacccgg
    10081 gaacctacct ggccggtggg tacgggcggc tcgagaccga gatcgcgggg cgagtagtgg
    10141 agaacgagga tctcgtcaac cttccgaact ggctggtcct gtcgttccgg gtggagggcg
    10201 gggagtggtt cgacctccgc ggggtggaga tcctcgacta cgtgcaggac ctggatctcc
    10261 gacagggggt gctggtccgc cggctgcgat tccgcgacgc tgaggggcgg gtgacggatc
    10321 tctcgcagcg ccgtttggtc cacatggagg acgcgcacct tgcggcactg cagacgacct
    10381 tcgtcgcgga gaactggagc ggccggattg aggtgcggtc ggggatcgac gggaccgtga
    10441 ccaactccgg cgtggcccgc taccgggacc tccgcggcga ccatctggtc cctcagggga
    10501 cggggtcgat cggggaggac ggcatctggt tggtggtcga gacgaggacc gggaagctac
    10561 gcgtggccga ggcggtgcgg acgcgcgtcc tgcgggacgg gacacccgcc gatgtgcctc
    10621 gccgtttggt ggagcgggag gggttcgtcg cgcacgacct cgacgtcgac ctcccgcgcg
    10681 gggagcccgt caccgtcgac aaggtggtcg cccttgccac ggggcgcggc cgcccgggct
    10741 acgagccggt catcgacgcc acgacctacg tacagcgggc gggcaccttc gaggacctgc
    10801 tcccctccca cgtgctcgcg tgggacctgc tatggggccg gttcgacctc tcgctgaacg
    10861 gacccgaacg ggcgcagctg gtccttcgtg tccacatctt ccacctgctc cagaccgcct
    10921 ccgagcacac catcgacctc gacgtcgggg ttcccgctcg cggactccac ggcgaggcct
    10981 accgcggcca tatcttctgg gacgagctgt tcatcttccc gttcctcaac taccggctac
    11041 cggcgctcac caggtcgttc ctcgaatacc ggcatcgccg gctccccgag gcgcgggccg
    11101 ccgcccgggc cgccggctac gacggcgcca tgtatccgtg gcagagcggc agcaccggcc
    11161 gcgaggagag ccagaccctc cacctcaacc cggagtccgg gcgatggctc ccggacaact
    11221 cgcatctgca gaagcacatc aatctcgcgg tcccgtataa cgtctggcag cactaccagg
    11281 tgaccggcga cctcgagttc ctccggttcc acggggccga catgcttatc gagatcgcga
    11341 ggttctggtc ctcgatcgcc acgtacaacc gcagcctcga ccggtacgag atcctc
//
LOCUS       DASUTW010000046         5433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_274744, whole genome shotgun sequence.
ACCESSION   DASUTW010000046 DASUTW010000000
VERSION     DASUTW010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274744"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VF058_01660"
     CDS             complement(<1..421)
                     /locus_tag="VF058_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEX6207041.1"
                     /translation="MTPKRELRPVDETQPTTFYGPTYLKNEEFRKAVGAVDFAVDARY
                     AWDTGVAISRFLEGLKEGRILGRECRSCGRTLVPPRMFCEECFRPTDRWVEVPDHGTV
                     NTFSICYIRWDMVELEEPELPFVLELDVDTPMMGFMHK"
     gene            complement(418..1581)
                     /locus_tag="VF058_01665"
     CDS             complement(418..1581)
                     /locus_tag="VF058_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010866978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HEX6207042.1"
                     /translation="MGQRVAAVGAGMTRFVRRAQETGKELSWQAARAALEAADMTIDD
                     VDCVVMGTAPDAFDGVHMKSEYLADGAGAWGKPYYRTYVGGGTGVFSTIQGWWQVASG
                     QFDTCLVVAEEKMSSTQPHPQGAFITIFDNMIERPLGPNLLWIFALEMNRYMQVYGLD
                     KADIAAVSVKNKRNAADHPAALLGDADITVEDVLNSETLAWPVQRLDVSPVTDGAVAV
                     VLAADHVAKKKSPMPVWVEGVGWHLDTAYWTNRDLVYPRYVEYAARQAYEMAGIREPR
                     KEIHVAEPYDPFDYKELHHLEGLQLFEKGKAPVAMRDGVLNRDGDLPSCPSGGLLGVG
                     NPIAAAGLMKIAELYWQISGQAGKRQVPGDPRRGVAQAWGDLMQVGTVVVMGR"
     gene            complement(1581..1952)
                     /locus_tag="VF058_01670"
     CDS             complement(1581..1952)
                     /locus_tag="VF058_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6207043.1"
                     /translation="MPGLRRLDHVYYWTQDMDRAVKFYRDVLGLELIRREGSSWAEFE
                     TGTIRIALHGAVEGRPAEAGGATAVFEVDDLDATRAALEESGVELLDHMGEVEGYARF
                     ASFLDPDGNTVQVIEYAKGAS"
     gene            complement(1952..3118)
                     /locus_tag="VF058_01675"
     CDS             complement(1952..3118)
                     /locus_tag="VF058_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEX6207044.1"
                     /translation="MNDRVAIVGVGHAGFAPITAGVSYKELMFEAAMRAYDDAGVDVR
                     RDVDSFIGCSEDFWEGTSIFDEYLPDQIGGALRSVHTVSADGLFGIATALMLIRSGHA
                     EVVAVESHSKASDILTPGSIHEFALDPVYNRPLRVTPHAVAGLEMHRYLEVSGTTEEQ
                     CALVVAKNRRMALDNPRAAYPTEVTAADALDSEPLAWPLKRLEVAERADGCVVMVLAS
                     EGRARDLTDAPVWVLGTGWSTGSASLESRDWDDLTYVRQAADRAYRTARIGHPTGVVD
                     LAEVDDRYAYRELMHLEALRLAHPGEAGAMLEDGVFGRDGEVPVNVSGGSLGQGNLFE
                     ANGLARALECVLQLRGEAGERQVEDAGVAVAQSWRGVPTATSAVAVLASDEAVA"
     gene            complement(3167..3814)
                     /locus_tag="VF058_01680"
     CDS             complement(3167..3814)
                     /locus_tag="VF058_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6207045.1"
                     /translation="MTAPDPEFIRRRTTEAVLADGTRVLLRPLVPEDGPKMVEAFERL
                     SPESRYRRFMSPVDELSPEMVRKLTDVDYVDHFAWAALAMDEPGGPGIGVARYMRAED
                     DPQVAEAAVTVIDEYQGRGLGSLLLQALGASALEHGITRFRGYVLEDNRAMRDILEEL
                     GGTAHHDGAGIVRVEVELPIGEDLRSSPAYRALRALARGEGPRLLRWGALWDRPG"
     gene            complement(3811..4425)
                     /locus_tag="VF058_01685"
     CDS             complement(3811..4425)
                     /locus_tag="VF058_01685"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX6207046.1"
                     /translation="MAFPSRMSEGEHQVLALRPHWLGVVAPLVITVLVLVGMAAALLS
                     IPESWPSWVRWIVVGAFSLILLLWPLRRIGAWVTSEYVITNERVVHRQGWLTKRSVEI
                     PIMGVADVVFSQSVLQRLVRVGDVVIESAGGLGFTRLASIPRADDVHRTLYDLIEDAR
                     VVTSNNHGPPGPLDQIERLARLRERGLLTPQEFEIHKQRLLKQL"
     gene            complement(4487..5305)
                     /locus_tag="VF058_01690"
     CDS             complement(4487..5305)
                     /locus_tag="VF058_01690"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX6207047.1"
                     /translation="MGSETLSQDSLRAAVRAAGIEAPPRYHASIGSTNTEAARLAAEG
                     APAWTVVAAGHQTEGKGRLGRRWVEAPGRALLMSVLLRPRIDAVEAPLISLLAALSMA
                     EACRELTAAEVGCKWPNDLVIGERKVGGVLAEARIEGGEVVHVVVGTGVNARMRAEDF
                     PPELRATATSLAVEGEAVHPALLVRRYLERLREGLGPEGPTPALLDAYRERCASLRRT
                     VRATTTEGEEVEGMAVDVDERGGLVIESARGRRVVAFGEVAHVVPQGLPTRPEA"
     gene            5337..>5433
                     /locus_tag="VF058_01695"
     CDS             5337..>5433
                     /locus_tag="VF058_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX6207048.1"
                     /translation="MGKPRVLIANRGEIAVRVIRTCRELGIPTVAV"
BASE COUNT          772 a         2068 c         1827 g          766 t
ORIGIN      
        1 gcttgtgcat gaagcccatc atcggggtgt cgacgtcgag ctccaggacg aacggcagct
       61 cgggctcctc gagctcgacc atgtcccagc ggatgtagca gatcgagaac gtgttgacgg
      121 tgccgtggtc ggggacctcc acccagcggt cggtggggcg gaagcactcc tcgcagaaca
      181 tccgcggcgg cacgagcgtc cgcccgcacg agcggcactc gcggcccagg atcctgccct
      241 ccttcagccc ctccaggaag cggctgatgg ctacgcccgt gtcccaggcg tagcgggcat
      301 ccacggcgaa gtcgaccgcg cccaccgcct tccggaactc ctcgttcttg aggtaggtgg
      361 gcccgtagaa cgtcgtgggc tgggtctcgt ccacggggcg gagctctcgc ttcggcgtca
      421 tcggcccatc accaccacgg tgccgacctg catcaggtcg ccccaggcct gggccacgcc
      481 ccggcgcggg tcccccggca cctgcctctt gccggcctgc cccgagatct gccagtagag
      541 ctcggcgatc ttcatgaggc ccgccgccgc gatcgggttc cccacaccca gcaggccgcc
      601 cgaggggcac gagggcaggt cgccgtcgcg gttcagcacg ccgtctcgca tcgccacggg
      661 cgccttcccc ttctcgaaga gctggagccc ctcgagatgg tgcagctcct tgtagtcgaa
      721 cgggtcgtag ggctcggcga cgtggatctc cttgcgcggc tcgcggatcc ccgccatctc
      781 gtacgcctgg cgcgccgcgt actcgacgta gcgcgggtac acgaggtccc ggttcgtcca
      841 gtaggcggtg tcgaggtgcc agcccacgcc ctcgacccag accggcatcg gggacttctt
      901 cttcgccacg tggtcggccg cgagcaccac ggccaccgcg ccgtcggtga ccggtgagac
      961 gtcgagacgc tggaccggcc aggccagcgt ctcgctgttc aggacgtcct ccaccgtgat
     1021 gtcggcgtcc ccgagcaggg cggcggggtg gtcggccgcg ttccgcttgt tcttcaccga
     1081 gaccgccgcg atgtcagcct tgtcgagccc gtagacctgc atgtagcgat tcatctccag
     1141 ggcgaagatc cacagcaggt tggggccaag cggccgctcg atcatgttgt cgaagatcgt
     1201 gatgaacgcg ccctgggggt ggggctgggt cgaggacatc ttctcctcgg ccacaacgag
     1261 acacgtgtcg aactggcccg aggccacctg ccaccagccc tggatcgtgg agaagacacc
     1321 ggtcccgccg ccgacgtagg tccggtagta gggcttcccc cacgcgccgg cgccgtcggc
     1381 caggtactcc gacttcatgt ggaccccgtc gaaggcgtcg ggggcggtgc ccatcaccac
     1441 gcagtcgacg tcgtcgatcg tcatgtcggc cgcctcgagc gcggcccggg ccgcctgcca
     1501 cgacagctcc ttgcccgtct cctgcgcccg ccggacgaac ctcgtcatcc cggcgccgac
     1561 ggccgcgacc cgctggccca tcaagacgct cccttcgcgt actcgatcac ctgcaccgtg
     1621 ttgccgtcgg ggtcgaggaa ggaggcgaag cgcgcgtagc cctccacctc gcccatgtgg
     1681 tcgaggagct ccaccccgct ctcctcgagc gccgcccgcg tcgcgtcgag gtcgtccacc
     1741 tcgaagacgg ccgtggcgcc gccggcctcc gccggcctcc cctccaccgc gccgtggagg
     1801 gcgatccgga tcgtccccgt ctcgaactcg gcccagctcg acccctcccg ccggatcagc
     1861 tcgaggccca ggacgtcgcg gtagaacttc acggctcggt ccatgtcctg ggtccagtag
     1921 tagacgtggt cgagccgccg aagccccggc atcacgccac cgcctcgtcg gaggcgagca
     1981 ccgccacggc cgaggtcgcg gtggggacgc ctcgccacga ctgcgccacg gccaccccgg
     2041 cgtcctcgac ctgccgctcc cccgcctcgc cccggagctg cagcacgcac tccagcgccc
     2101 gggccaagcc gttcgcctcg aagaggttcc cctggcccag cgagccgccg gagacgttga
     2161 ccggcacctc accgtctcgc ccgaagaccc cgtcctcgag catcgcgccg gcctcaccgg
     2221 ggtgggccag ccgcagggcc tccaggtgca tcagctcccg atacgcgtag cggtcgtcga
     2281 cctcggccag gtccacgacg cccgtcgggt ggccgatccg cgccgtccgg tacgcacggt
     2341 cggccgcctg tcggacgtag gtgaggtcgt cccaatcccg gctctccaac gacgccgagc
     2401 ccgtggacca gccggtgccc agcacccaca ccggcgcgtc ggtgaggtcc cgggcccggc
     2461 cctcgcttgc cagcaccatc acgacgcagc cgtcggcccg ctcggccacc tcgagccgct
     2521 tcaggggcca ggccagcggc tcggagtcga gcgcgtccgc cgccgtgacc tctgtggggt
     2581 aggcggcgcg ggggttgtcc agggccatcc gccggttctt cgccaccacg agcgcgcact
     2641 gctcctcggt cgtccccgag acctcgaggt agcggtgcat ctcgaggccc gcgacggcgt
     2701 gcggcgtgac gcggagcggc cggttgtaga cggggtccag cgcgaactcg tggatcgagc
     2761 cgggggtgag gatgtccgag gccttgctgt ggctctccac ggccacgacc tcggcgtgcc
     2821 cggagcggat caacatcagg gcggtggcga tcccgaacag cccgtcggcc gacacagtgt
     2881 ggaccgaccg gagggcgccg ccgatctgat cggggaggta ctcgtcgaag atcgaggtgc
     2941 cctcccagaa gtcctcggag cacccgatga aggagtccac gtcccggcgg acgtccacgc
     3001 cggcgtcgtc gtaggcgcgc atcgcggcct cgaacatcag ctccttgtac gagacgccgg
     3061 ccgtgatggg ggcgaacccc gcgtggccga cgccgacgat cgcgacgcgg tcgttcatgg
     3121 gcgcgcgcag tctagcaaag ggcccagagc caccacgaat cccggctcac ccaggacgat
     3181 cccacagcgc cccccaccgc agcagccgcg gcccctcgcc cctggccagc gcgcgcaggg
     3241 cgcggtaggc gggcgaggag cgcaggtcct ccccgatcgg aagctcgacc tcgacccgga
     3301 cgatccccgc tccgtcgtgg tgcgccgtcc cccccagctc ctccaggatg tcccgcatcg
     3361 cccggttgtc ctccaggacg tagccccgga accgggtgat cccgtgctcg agggcgctcg
     3421 ccccgagcgc ctgcagcagc agcgagccga gcccccgccc ctggtactcg tcgatcacgg
     3481 tgacggccgc ctcggccacc tgcggatcgt cctcggcgcg catgtatcga gcaaccccga
     3541 tcccgggccc gccgggctcg tccatcgcca gggccgccca cgcgaagtgg tcgacgtagt
     3601 ccacgtcggt cagcttgcgg accatctcgg ggctgagctc gtccaccggc gacatgaatc
     3661 tccggtacct gctctcgggc gacagccgct cgaaggcctc caccatcttc ggcccgtcct
     3721 cgggcacgag ggggcgcagg agcaccctgg tgccgtcggc gaggaccgcc tcggtggtgc
     3781 ggcgccggat gaactccggg tccggggcgg tcacagctgc ttgaggagcc gctgcttgtg
     3841 gatctcgaac tcctgggggg tcagcaggcc gcgctcgcgc agccgcgcca gccgctcgat
     3901 ctggtcgagg ggaccggggg ggccgtggtt gttcgaggtg acgacgcggg cgtcctcgat
     3961 caggtcgtag agcgtccggt ggacgtcgtc ggcccggggg atggaggcga gcctggtgaa
     4021 gccgaggccg ccggccgact cgatcaccac gtcccctacc cgaacgagac gctggaggac
     4081 cgactggctg aagacgacgt cggccacccc catgatcggg atctcgaccg atcgcttggt
     4141 cagccagccc tgccggtgca ccacccgttc gttcgtgatg acgtactcgg aggtgaccca
     4201 agccccgatc ctgcggagcg gccacaggag gaggatcagg gagaacgccc cgaccacgat
     4261 ccaccggacc cacgacggcc agctctcggg gatgctgagc agcgccgccg ccatcccgac
     4321 caggacgagg acggtgatga cgagcggcgc gaccaccccc agccagtgag ggcggagcgc
     4381 gaggacctgg tgctcgccct cggacatccg gctcggaaag gccactccgt acctcctctt
     4441 cgcggccggt ccaaggtaag ccgggtcggg cctcacggca agagagtcac gcttcgggcc
     4501 gcgtcggcag gccctgcggg accacgtggg ccacctcgcc gaaggcgacc acgcgccgac
     4561 cccgggccga ctcgatgacg agccctcccc gctcgtcgac gtccacggcc atcccctcga
     4621 cctcctcacc ctcggtcgtc gtggcccgga cggtgcgccg cagggaggcg caccgctccc
     4681 ggtaggcgtc gaggagcgcg ggggtcggcc cctcaggccc gagcccctcc cggaggcgct
     4741 ccaggtaccg gcggaccagc agggcgggat gaacggcctc cccctcgacc gccaggctcg
     4801 tcgccgtggc gcggagctcc ggagggaagt cctcggcccg catccgcgcg ttgacccccg
     4861 tgcccacgac gacgtggacc acctcccccc cctcgatccg agcctccgct agcacgcccc
     4921 ccaccttccg ctccccgatc acgaggtcgt tgggccactt gcaccccacc tcggccgccg
     4981 tgagctcccg gcaggcctcg gccatcgaga gcgcggcaag gagggagatc aggggggcct
     5041 cgaccgcgtc gatccggggc cgcagcagga cgctcatcag gagggcccgg cccggcgcct
     5101 ccacccaccg ccgccccagc cgacccttcc cctcggtctg gtgccccgcg gccaccaccg
     5161 tccaggcggg ggcgccctcc gcggccagcc gcgcggcctc cgtgttcgtg gagccgatgc
     5221 tcgcgtggta gcggggaggg gcctcgatcc ctgcggcacg gacggctgcg cgcaggctat
     5281 cctgactcag cgtctcggag cccatgggcg ggcccgatta tctcaggtgg ggtctgatgg
     5341 ggaagccacg cgtcctgatc gccaaccgcg gcgagatcgc cgtccgggtg atccgtacat
     5401 gccgggagct cggcatcccc accgtcgccg tct
//
LOCUS       DASUTW010000047         2659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_274749, whole genome shotgun sequence.
ACCESSION   DASUTW010000047 DASUTW010000000
VERSION     DASUTW010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274749"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VF058_01700"
     CDS             complement(<1..465)
                     /locus_tag="VF058_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207049.1"
                     /translation="MTSIPPPSFASSLGRRTLVIVAAAGLIAAPALLLRAFCVGESCR
                     RQAAPEVRAPFCSLDPQLRRLVADGYRQGRSPEVLVVTSEDATVAGGLENLSAPWPSV
                     GDVDAGRVPLVFHGAGIDPAAEVPTATPLVDLAPTAAAILDVRVPHPGVRSGE"
     gene            511..2208
                     /locus_tag="VF058_01705"
     CDS             511..2208
                     /locus_tag="VF058_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6207050.1"
                     /translation="MMIRALVLLVAVLSASCSGPPDPPPGARSIEEMAREVGADVLEA
                     TRRGYVPGRSGELALIPRPHHVIVRRGSWDPGREPSPDTSHSTPWAYHQRVPILLYGP
                     GFVREGVVSDRAVDLADLAPTLAELMGFPFRARDGEPLFEALASGRRGAPRAIALVAY
                     DGGGWNLLQAYPDDWPILRALMARGALFTNATVGSSPSVTAAVHPTMGTGAYPAAHGV
                     PDNSARLPDGTVGDAYLGRTDPGLLRVETLADAWHEATGAWTGIVATESWHLGMLGRG
                     AEPGAAPEQVAVLWNRRERRFFTNRERYRLPGYLPDDASLGARVRDLDGSDGSLDGRW
                     MGNDLEDPDVLPATPAFVRHQGEAILQILGRERIGRDDVTDLLFVELKSTDRGAHLWN
                     VAGEEQPHVLRAQDDLLRDVAAALDAEAGEGRWVLAVTADHGLTPLPEEAGGLRLHPE
                     ALAAAVEERLGPIVEAATPSSLFLDMDEVRSRDLDLELVATIVGDLRYRDGIPEGVVL
                     ERIREDLLDRRVFAAVLPGPYLEGLTPEETARVGPGIYEEGDLTGTDAAARFARLAG"
     gene            complement(2162..>2659)
                     /locus_tag="VF058_01710"
     CDS             complement(2162..>2659)
                     /locus_tag="VF058_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207051.1"
                     /translation="GAGALGAVVGFVVAHALKDRVPPARIVSGALLFGAAGMLAFGGV
                     INMLGISALAFCVGLAFFLGKVAVDTLMQQALTDSYRGRGFSLQDVSYNLSWVLPALL
                     LWLVLTPETARMVLIGSGAAFLLIALLIGLWSRALGGRPERVAPAAEEVSPPASRTSP
                     PRPSP"
BASE COUNT          372 a          984 c          993 g          310 t
ORIGIN      
        1 ctcccccgag cggacccccg ggtgggggac ccggacgtcg aggatggcgg ccgcggtcgg
       61 ggccaggtcg acgagaggcg tggcggtagg gacctcggcg gcggggtcga tccccgcccc
      121 gtggaagacg agcggcacgc ggccggcatc cacgtccccc acggagggcc acggcgcgga
      181 gagattctcg agcccgccag ccacggtggc gtcctcgctc gtgacgacga ggacctctgg
      241 ggagcggccc tgccggtagc cgtcggccac caggcgacgg agctgggggt cgagggagca
      301 gaacggcgcc cggacctccg gggcggcctg ccggcggcag gactcgccta cgcagaaggc
      361 gcggaggagg agagccggcg ccgcgatgag acccgcggcc gcgacgatga ccagggtgcg
      421 acggcccagg ctgctcgcga aggacggcgg ggggatggag gtcatgtgcc tcgggccacg
      481 atacacgagc ctccctgcgg gatactccgg atgatgatcc gggcgctcgt cctcctcgtg
      541 gcggtgctct cggcgtcgtg ctcgggaccg ccggaccctc cgccgggggc gcggagcatc
      601 gaggagatgg cgcgcgaggt gggggccgac gtgctggagg cgacgcggcg cgggtacgtc
      661 cccggccgct ccggcgagct ggccctgatc cccaggcccc accacgtgat cgtgcgacgg
      721 ggaagctggg acccgggccg ggagccgagc cccgacacca gccactcgac cccgtgggcc
      781 taccaccagc gggtgccgat cctcctttac ggaccggggt tcgtccgcga aggcgtcgtc
      841 tcggaccgag cggtcgacct ggcggacctg gccccgacgc tcgccgagct catgggcttc
      901 ccgttccgag ctcgggacgg cgagcccctg ttcgaggcgc tggcatcggg gcgccggggg
      961 gcgccgcggg ccatcgcgct cgtggcctac gacggcggtg ggtggaacct cctccaggcc
     1021 tatcccgacg actggcccat cctgcgagcg ctgatggccc gcggcgccct gttcacgaac
     1081 gccacggtgg gctcctcgcc ctcggtcacc gcggcggtgc accccacgat gggcacgggc
     1141 gcctacccgg ccgcccacgg ggtcccggac aactccgccc gtctcccgga cggcacggtg
     1201 ggggacgcgt acctcggccg aaccgatccc gggctgctgc gcgtcgagac gctcgccgac
     1261 gcgtggcacg aggcgaccgg cgcctggacg gggatcgtgg ccacggagtc ctggcacctg
     1321 gggatgctcg ggcgcggcgc cgagcccggc gccgcgcccg agcaggtggc cgtcctctgg
     1381 aaccgccggg agcggcgctt cttcacgaac cgcgagcggt accgcctccc cggatacctc
     1441 cccgacgatg cgtcgctcgg ggcccgcgtc cgggaccttg acgggagcga cgggtcgctc
     1501 gacggccggt ggatggggaa cgacctcgag gaccccgacg tgctcccggc cacccccgcc
     1561 ttcgtccggc atcagggcga ggcgatcctc cagatcctgg gtcgggagcg gatcgggcgc
     1621 gacgacgtaa ccgacctcct cttcgtcgag ctcaagtcca cggaccgggg cgcgcatctc
     1681 tggaacgtgg cgggtgagga gcagccacac gtccttcggg cccaggacga cctcctgcgg
     1741 gacgtggccg ccgccctcga cgccgaggcg ggggaggggc ggtgggtcct ggcggtcact
     1801 gccgaccacg ggctcacgcc cctgcccgag gaggccggcg ggctccgtct ccaccccgag
     1861 gcgctcgcgg ccgccgtcga ggagcgactc ggcccgatcg tggaggcggc cacgccctcc
     1921 tcgctcttcc tcgacatgga cgaggttcgc tcccgcgacc tcgatctcga gctcgtggcc
     1981 acgatcgtgg gcgatctccg ctaccgggac gggatccccg agggcgtagt cctcgagcgg
     2041 atccgcgagg acctcctcga ccgacgggtg ttcgccgcag tcctgccggg accgtacctc
     2101 gaaggcctca cgccggagga gaccgcccgc gtcggccccg ggatctacga ggagggcgac
     2161 ctcacgggga cggacgcggc ggcgaggttc gcgaggctgg cgggctgacc tcctcggccg
     2221 cgggggccac ccgctccggc cggccgccca gcgccctcga ccacagcccg atcagcaggg
     2281 cgatcagcag gaaggccgcc cccgagccga tcagcaccat ccgagccgtc tcgggcgtga
     2341 ggaccagcca gagcaggagg gcggggagca cccacgagag gttgtaggag acgtcctgga
     2401 ggctgaaccc ccggccgcgg tacgagtcgg tcagcgcctg ctgcatcaag gtgtccacgg
     2461 ccaccttccc caggaagaac gccaggccga cgcagaaggc cagcgctgag atcccgagca
     2521 tgttgatcac gcccccgaac gccagcatcc ccgccgcccc gaacagtagc gcccccgaca
     2581 cgatcctggc cggcggcacg cggtccttca gggcgtgggc gaccacgaac cccacgacgg
     2641 cgcccagcgc gccggcgcc
//
LOCUS       DASUTW010000048         3601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_663797, whole genome shotgun sequence.
ACCESSION   DASUTW010000048 DASUTW010000000
VERSION     DASUTW010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663797"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      <1..114
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            164..1258
                     /gene="ribD"
                     /locus_tag="VF058_01715"
     CDS             164..1258
                     /gene="ribD"
                     /locus_tag="VF058_01715"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX6207052.1"
                     /translation="MDDAYMRRALELAERGRGLVSPNPLVGAVVVAGGEVVGEGWHEG
                     PGTAHAEIRALEAAGPRARGATLYVSLEPCSHHGRTPPCAPRVADAAPVRVVAAITDP
                     NPLVDGASLRLLRERGVEVTLGVLAEEASRQNRAFLRHIRTGLPFVTLKMAASLDGKA
                     AARDGSSRWITGEEARADVHLLRASADAILVGAGTAFRDDPSLTVRLPSYRGAPKLRV
                     VVDGRGIVPDTHRLFDGEAPTLVATTERAPRGRREAWRRAADVLELEDAGDGLIPLDR
                     LMQELGKRDVQHVLVEGGPTLAWEMVERDVVDELILYVAPVLVGGEGAPSVLMGGGTP
                     SIGDGRAVTIREVSRMGPDLKVVADVHRDR"
     gene            1239..1817
                     /locus_tag="VF058_01720"
     CDS             1239..1817
                     /locus_tag="VF058_01720"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX6207053.1"
                     /translation="MFTGIVEERGVVRASAPRLQIEAASVSADARTGDSISVDGVCLT
                     VVDRAEGTLAFDVAPETVRRTTLGDLAEGNAVNLERPVTLATRLGGHLVQGHVDGVGE
                     IASVVPEGDGVRMTVRVPEGLGRYLVEKGSVAVDGVSLTLAEVRPDGFAVALIPHTLE
                     ATGLGGKRPGDRVNLEMDLIAKYVERLMRRDR"
     gene            1814..3073
                     /locus_tag="VF058_01725"
     CDS             1814..3073
                     /locus_tag="VF058_01725"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX6207054.1"
                     /translation="MKQEEDVGLVEGVFASIEEAVEDIAQGKIVIVVDDADRENEGDF
                     VMAAEKVTPEAINFMATHGRGLVCLPSAGWRLDELGIPLMVQRSEGSQETAFAVSIDA
                     KDVTTTGTSAHDRAATCRAVTEPSTRSEDLLMPGHVFPLRAQEGGVLRRAGHTEAAVD
                     LASMAGLYPVGVICEIMHPDGTMARLPELVRVAREHDLKIISIADLIEHRRRREQLIE
                     KVAEATIPTIYGEFRAQVYESKIDGRQHVAMVLGRVGDGRDVMVRVHSECLTGDVFHS
                     LRCDCGTQLDSALGKIASEGRGVVLYIRGHEGRAIGLKHKLQAYSLQEQGKDTVEANL
                     ELGFPPDPRDYGVGAQILYDLGVRTMRLLTNNPSKRAGLEGHGLDIVERVPLETKPTA
                     QNLDYLRAKREKLGHLLESLEADGEGS"
     gene            3073..3549
                     /gene="ribH"
                     /locus_tag="VF058_01730"
     CDS             3073..3549
                     /gene="ribH"
                     /locus_tag="VF058_01730"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX6207055.1"
                     /translation="MAREGDLDARGRRFAVVVGRFNQVVTERLLEGARSAFRHHGVAD
                     EDVEVAWVPGSFEIPVVARRFARSGEVEAVVCLGAVIRGETAHFDVVARESAGGAQQV
                     ALETGVPCILGILTTDTLDQALERAGGKHGNKGWDAAVAAIEIASLMSSLPKERDA"
BASE COUNT          525 a         1242 c         1367 g          467 t
ORIGIN      
        1 ggggtggaac tccccaccgg tggtgacagt ccacgacccc ccgcgagcca cgcgggggtg
       61 gacccggcgg aatcccggga ccgacggtga cagtccggat gagagaaggc gaggacggcg
      121 gcgtctgccg cccgttcccc ccttcccccg agagaggaac gcggtggacg acgcgtacat
      181 gcggagggca ctcgagctgg ccgagcgagg ccgcgggctc gtctcgccca accccctcgt
      241 cggcgccgtc gtggtggctg gcggcgaggt cgtgggggag gggtggcacg agggccccgg
      301 gaccgcccac gccgagatcc gggcgctcga ggcggccggg ccgagggctc ggggcgcgac
      361 cctctacgtg agcctggagc cctgcagcca tcacggccgc acgccgccct gcgccccacg
      421 cgtggccgac gccgccccgg tccgggtcgt ggcggcgatc accgacccca atcccctcgt
      481 ggacggcgcc agcctccgcc tcctccggga gcggggtgtc gaggtgacgc tcggcgtcct
      541 ggccgaggag gcctcccggc agaaccgggc cttcctgcgg cacatccgca cgggcctccc
      601 gttcgtcacc ctgaagatgg ccgcctccct ggacgggaag gccgccgcgc gcgacgggtc
      661 ctcgaggtgg atcacgggcg aggaggcccg tgcggacgtg cacctcctcc gggcctcggc
      721 cgacgcgatc ctggtggggg cgggcacggc gttccgggac gacccgagcc tgaccgtcag
      781 gcttccctca taccgcggcg cccccaagct gcgcgtggtg gtggacgggc gggggatcgt
      841 gcccgacacg caccgcctct tcgacggaga ggcccccacg ctcgtggcca cgaccgagcg
      901 cgccccgagg gggcggcgcg aggcgtggcg ccgggcggcc gacgtgctcg agctcgagga
      961 cgccggggac ggcctgatcc cgctggatcg gctgatgcag gagctcggga agcgcgacgt
     1021 ccagcacgtg ctcgtcgagg gcggtcccac gctggcctgg gagatggtgg agcgagacgt
     1081 cgtggacgag ctgatcctct acgtggcccc ggtgctcgtt ggcggggagg gagctcccag
     1141 cgtgctgatg gggggtggga cgccctcgat cggggacggg cgggccgtga cgatccgaga
     1201 ggtctctcgc atgggacccg acctcaaggt ggtggcggat gttcaccggg atcgttgagg
     1261 agcggggggt ggtgcgcgcg tccgcgcccc gcctccagat cgaagccgcc tcggtgagcg
     1321 cggacgcgcg caccggggac tcgatcagtg tggacggcgt ctgcctgacc gtcgtggacc
     1381 gcgctgaggg gacgctcgcc ttcgacgtgg ccccggagac cgtgcgccgg accacgctgg
     1441 gagacctggc cgaggggaac gccgtgaacc tggagcgtcc cgtgacgctc gccacccgcc
     1501 ttggagggca tctcgtccag gggcacgtgg acggggtggg cgagatcgcc tcggtcgtgc
     1561 ccgagggcga cggcgtccgg atgacggtgc gcgtccccga gggcctgggc cggtacctcg
     1621 tcgagaaggg ctcggtggcc gtggacggcg tcagcctcac cctggccgag gtgcggccgg
     1681 acgggttcgc cgtggcgctg atcccccata ccctcgaggc caccggcctg ggcggcaagc
     1741 ggccgggcga ccgggtcaac ctcgagatgg acctgatcgc gaagtacgtg gaacgactga
     1801 tgcggaggga tcgatgaagc aggaggagga cgtcggcctg gtcgagggcg tgttcgcctc
     1861 gatcgaggag gccgtcgagg acatcgccca gggcaagatc gtcatcgtcg tcgacgacgc
     1921 cgaccgcgag aacgaaggag acttcgtgat ggcggccgag aaggtgacgc ccgaggcgat
     1981 caacttcatg gccacccacg gccgcgggct ggtctgcctc cccagcgcgg gatggcggct
     2041 ggacgagctg gggatccccc tcatggtcca gcgatcggag ggctcccagg agaccgcgtt
     2101 cgccgtctcg atcgacgcca aggacgtgac gacgaccggg acgagcgcgc acgaccgggc
     2161 cgcgacgtgc cgcgccgtca ccgagccctc caccaggtcc gaggacctcc tgatgccggg
     2221 ccacgtcttc ccgctccggg cccaggaagg cggcgtcctc cgccgggcgg gccacaccga
     2281 ggccgcggtc gacctggcct cgatggcggg gctgtatccg gtgggcgtga tctgcgagat
     2341 catgcacccc gacgggacga tggcccgcct ccccgagctc gtgcgggtgg cccgcgagca
     2401 cgacctcaag atcatctcga ttgccgacct gatcgagcac cgcagacggc gggagcagct
     2461 gatcgagaag gtggccgagg ccacgatccc cacgatctac ggcgagttcc gggcccaggt
     2521 ctacgagtcg aagatcgacg gccgccagca cgtggccatg gtcctgggcc gggtcggcga
     2581 cggacgcgac gtcatggtcc gggtccactc cgagtgcctc accggcgacg tcttccacag
     2641 cctgcgctgc gactgcggca cgcagctcga ctccgctctg gggaagatcg ccagcgaggg
     2701 ccggggcgtg gtgctctaca tccgcggcca cgaggggcgg gccatcggcc tcaagcacaa
     2761 gctccaggcc tacagcctcc aggagcaggg gaaggacacc gtcgaggcca acctcgagct
     2821 cggcttccct cccgatcccc gcgactacgg tgtgggggcg cagatcctct acgacctcgg
     2881 ggtccggacg atgcggctcc tcacgaacaa cccctccaag cgagcggggc tcgagggcca
     2941 cgggctggat atcgtggagc gcgtgcccct ggagacgaag cccacggccc agaacctcga
     3001 ctacctccgg gcgaagcgcg agaagctggg gcacctcctt gagagcctgg aggcagacgg
     3061 ggaggggtcg tgatggcgcg cgagggcgac ctcgacgctc gcggccgccg cttcgccgtg
     3121 gtcgtgggcc gcttcaacca ggtcgtcacc gagcgcctcc tggagggggc ccggtcggcc
     3181 ttccgccatc acggtgtggc cgacgaggac gtcgaggtgg cctgggtccc cgggtccttc
     3241 gagatccccg tcgtggcccg ccgcttcgct cgctcgggcg aggtcgaggc ggtggtctgc
     3301 ctcggcgcgg tcatccgggg ggagacggcc cacttcgacg tggtggcgcg ggagtcggcc
     3361 ggcggggccc agcaggtggc gctggagacc ggcgtgccct gcatcctcgg catcctcacg
     3421 accgacacgc tcgaccaggc gctggagcgg gcgggcggca agcacggcaa caaggggtgg
     3481 gacgcagcgg tggccgcgat cgagatcgcc agcctgatga gctccctccc gaaggagcgt
     3541 gacgcatgat cgtggacaag ccctggggca aggtcgccac ctacgccctc aaccagccct
     3601 c
//
LOCUS       DASUTW010000049         7377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_792278, whole genome shotgun sequence.
ACCESSION   DASUTW010000049 DASUTW010000000
VERSION     DASUTW010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7377
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792278"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1054)
                     /locus_tag="VF058_01735"
     CDS             complement(38..1054)
                     /locus_tag="VF058_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HEX6207056.1"
                     /translation="MRTARIAMALIVLSLSAAACGDGEAGEDQDVSLTVYSGRDEEFI
                     GPLYERFEDETGIDLEVRYGDSAELAATILEEGEASPADVFVSQDAGALGVVADQGLL
                     SEIDPEILDRVDERFRSPEGLWVGASGRARVIAYNTETVSESELPTTVLDLAGPAYEG
                     RVGFPPTNASFQAFVAAMIITEGEDATRSFLQGLADNDPRLFESNSDTVRAVAAGEVD
                     FGLVNHYYRYEVEAEDGPIPVENHFLAGGDPGALVNAAGAGVLASSDAPDASMRLLDF
                     LTGEEGQRYFATETFEYPVVPGIEPGEDLPPLAEIESPSIDLSDLGATLEPALRLLAE
                     VGLL"
     gene            1153..1380
                     /locus_tag="VF058_01740"
     CDS             1153..1380
                     /locus_tag="VF058_01740"
                     /inference="COORDINATES: protein motif:HMM:NF040017.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6186 family protein"
                     /protein_id="HEX6207057.1"
                     /translation="MTRLSATAAVLWGLIFAALLVVQGMGLVREGWPTLSDMLRAITR
                     HPVARWVLFGFWIWIGWHLFVRGWRFLLREP"
     gene            1377..1685
                     /locus_tag="VF058_01745"
     CDS             1377..1685
                     /locus_tag="VF058_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6256 family protein"
                     /protein_id="HEX6207058.1"
                     /translation="MSRILREVVPPLLTIYVLVVAMLVSLRRRWPPRRFERSPREVAL
                     TVSIGYLVFLLIVLFFMTWATGDETALPDAVWEGAALLAIAVPAFLALSAVDALRRRR
                     "
     gene            complement(1686..2903)
                     /locus_tag="VF058_01750"
     CDS             complement(1686..2903)
                     /locus_tag="VF058_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX6207059.1"
                     /translation="MARILLLLPATAYRADDFLEAAEALGVEVVVASDRRPALAAQME
                     DRALTVPMSDPDAAAKRIERFGREHAIDAVVPVDDRGVVVAAVAAERLDLPHNPQGAA
                     TAATEKLRMRRALAAAGVPQPGFRVLGPEDAAGDLADELGYPVVLKPVSLSMSRGVLR
                     ADSPREARAAAGHIREILARAREDPLGPLLVERYVPGQEMALEGLLLRGDLETLALFD
                     KPEPMEGPTFEETIYVTPSREPFEMQDSVRTLAAGAAKAMGLREGPIHAEVRLGPSGP
                     VVLEVAARSIGGLCSRSLRFGAGISLEEVILRHALGLPTDDLRRESAASGVLMLPVPS
                     AGTLVEVRGQDEAGAVPGIEGVTITIPPGGELVPLPEGDRYLGFVFARAETPAEVEAA
                     LREARDRLEPVIE"
     gene            2923..3639
                     /locus_tag="VF058_01755"
     CDS             2923..3639
                     /locus_tag="VF058_01755"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX6207060.1"
                     /translation="MSEQPKDELLTITAQAREALLGLRAQDPAPDSLGLRIAVAGERD
                     GRYVHDMSFELLEDAGPGDVVLQHDDIPMIVPQDSMKKLRGAVVDLAPDGQSWTIDNP
                     NRPKRMLPLAPLGGGHAGHDHAGHEQAASPAAQAEIPEELRGTLEGDTAQQVIEVLDR
                     HINPSIAMHGGHAELAGLKEGVAYLRLSGGCQGCSMAAVTLRQGIERSLRQLVPEIQG
                     VADVTDHASGTDPFYSSSKG"
     gene            complement(3640..4137)
                     /locus_tag="VF058_01760"
     CDS             complement(3640..4137)
                     /locus_tag="VF058_01760"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6207061.1"
                     /translation="MHETPEDLERLGKVLDESHAGGGGHLRSILQDERRISADELPEL
                     LPGVQVMVLSTVTSDCRPIAGPVDGLFYRGAFHFGTSAGSVRFNHLSRRPQVSAVHAR
                     GEELVIAVHGEARPADVTAAEHEGFRSYLLEVYPDWETWWGEAPPPYFRIEPRKMFAS
                     RLPGA"
     gene            complement(4144..6225)
                     /gene="fusA"
                     /locus_tag="VF058_01765"
     CDS             complement(4144..6225)
                     /gene="fusA"
                     /locus_tag="VF058_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX6207062.1"
                     /translation="MNTYEPNAIRNVLLVGHGGTGKTTLADALLFAAGATTRMGTVEE
                     GNTVTDFEAEEQKKQISVSLALAPFDWRATKINLLDAPGYADFIGDVQAALRAADACL
                     FVVSAVDGVQVQHEVVWEMAVEAGLPRAVFVNKIDRERASFERTLDQLVQAFGTQVAP
                     LQFPIGEEHDFEGVADLLSGRAYRYPSGPQSEESDWPDDIAGKAEPMREKLTEAVAEG
                     DDAMLEKYLEEGELSAEEITSGVKTGLLQARFVPVLVGSAASAKGVDRLADLIVDAFP
                     APTDRPPVKVMTKAGEEEEREADPNGPLTAFVFKSFSDPYVGQISLFRVLSGRMRPDS
                     TVHNATKGSDERIHQVFTLRGKEHENVSDVPAGDIAAVSKLSDTATGDTLTTKEAAAT
                     LPAVEWAEPLYAVAVHPKTQGDEDKLSTALQRIQDEDPTIRVERRVETGQTVVTGMGE
                     AHIDVLIERMHRKFGVEVTTEPAKVAFKETIRGKAEGLGRHVKQSGGHGQYGVCNIRI
                     EPLTRGEGFVFEDEIFGGALPNQFIGSVEKGIVKAMQEGVLAGFPVVDVKVTLYDGKY
                     HSVDSSDMAFQIAGALAFRDATQNAGVVLLEPIVDVEVLVPEGMTGDIMGDLNSKRGK
                     VQGMDPTGTGKTRIRAQVPLTEMTRYPIDLRSITGGHGTFSMTFSHYEEVPSHIADRL
                     VQELKAEGAEE"
     gene            6329..6847
                     /locus_tag="VF058_01770"
     CDS             6329..6847
                     /locus_tag="VF058_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207063.1"
                     /translation="MEQTIRPRPSSVWTILVGAVLAAASSAVFVLVDELQMAGAAAAV
                     AVLGVVVAGWSARESGEPRVGFAASMLERIVDAAILGAVAWSLLPERAGAAALVALAG
                     SYVAAYLEVKASGLGFEAGAWGLAWPLAMVVLAAGLVAGEAELGLWGAAAISIAAALA
                     RSVQVARQREPS"
     gene            6844..>7377
                     /locus_tag="VF058_01775"
     CDS             6844..>7377
                     /locus_tag="VF058_01775"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HEX6207064.1"
                     /translation="MTERETFAETFSYWYYRSAAWAARALPERVGRRLFSAAAAVEHA
                     RNHAQRRVVSRNLSRVLGKPPDSLEVRAAVREAFDSYGRYWYDTFLLRALAPELVRKR
                     FDMRGLEFVDAALEQGRGALFCLPHLGNWDVAGHWMTLQGYALTAVAETLRPERLFHL
                     FLEHRRDLGMNIVPLTGT"
BASE COUNT          960 a         2715 c         2569 g         1133 t
ORIGIN      
        1 gcccgcgccg cgggctcggc cgccgcgacc cccacgctca cagcagcccc acctcggcca
       61 gcaggcgcag cgccggctcc agcgttgccc cgaggtcgga gaggtcgatc gaaggcgact
      121 cgatctcggc cagcggcgga agatcctcgc ccggctcgat cccgggtacc accgggtact
      181 cgaaggtctc cgtggcgaag tatcgctggc cttcttcgcc cgtgaggaag tcgaggaggc
      241 gcatcgaggc atccggggcg tcggaggagg ccagcacgcc ggcgccggcc gcgttcacca
      301 gggcgccggg atcccctccc gccaggaagt ggttctcgac cgggatcggc ccgtcctccg
      361 cctccacctc gtacctgtag tagtggttca cgagcccgaa gtccacctct cccgcggcca
      421 cggcccgaac cgtgtcggag ttcgactcga acagcctcgg gtcgttgtcg gcgagcccct
      481 ggaggaagct gcgcgtcgcg tcctccccct cggtgatgat catggccgcg acgaaggcct
      541 ggaaggacgc gttcgtgggg gggaacccga cccgaccctc gtacgccggg ccggcgaggt
      601 ccagcacggt ggtcgggagc tcgctctcgc tcaccgtctc ggtgttgtag gcgatcaccc
      661 tggcccggcc cgaggccccc acccagaggc cctcggggga gcgaaaccgc tcgtccaccc
      721 ggtccaggat ctccgggtcg atctcgctca gcagcccctg gtcggccacg acccccaggg
      781 ctccggcgtc ctgggagacg aacacgtcgg ccgggcttgc ctcgccttcc tccaggatcg
      841 tggcggccaa ctccgcggag tccccgtagc ggacctcgag gtcgatcccg gtctcgtcct
      901 cgaaccgctc gtacagcggc ccgatgaact cctcgtcgcg gcccgagtac acggtgagcg
      961 agacgtcctg atcttcgccc gcctccccgt ccccgcaggc ggcggccgag agggacagga
     1021 cgatcagggc catggcgatg cgagcggtcc tcatcgatcg ttcccccgta aggtaaccct
     1081 aacgcccaac cttcgggcgg ccgaaaccct acctgctggg tcggaaattc gtcaagggct
     1141 atgctagccc gcatgacgcg gctgagcgcc acggcggccg tcctctgggg cctgatcttc
     1201 gcggcgctgc tggtcgtcca gggcatgggc ctggtgcgcg aaggctggcc caccctctcg
     1261 gacatgctcc gcgccatcac ccggcacccg gtcgcgagat gggtcctctt cggcttctgg
     1321 atctggatcg ggtggcacct gttcgtccgg ggctggcgct tcctgctccg cgagccgtga
     1381 gtcggatcct ccgcgaggtc gtcccgcccc tcctcacgat ctacgtcctc gtcgtcgcca
     1441 tgctggtctc cctccgccgc cgctggcctc cacggcggtt cgagcggtcg ccgagggagg
     1501 tggcgctgac ggtctcgatc ggctacctgg tgtttctgct gatcgtcctc ttcttcatga
     1561 cgtgggccac gggggacgag acggccctcc ccgacgccgt gtgggaggga gccgccctgc
     1621 tggcgatcgc ggtccccgcg ttcctggcgc tgtcggcggt cgatgccctc cggcggcgcc
     1681 gctgatcact cgatgacggg ctcgagccgg tccctcgcct cgcgcagcgc ggcctccacc
     1741 tcggcgggcg tctcggcacg ggcgaagacg aagccgaggt agcggtcgcc ctcgggcagc
     1801 gggacgagct cccctccggg cgggatcgtg atcgtcaccc cctcgatccc ggggacggcc
     1861 ccggcctcgt cctggccccg gacctcgacc agcgtcccgg ctgatggcac gggcagcatc
     1921 aggacgcccg aggccgccga ctcgcggcgg aggtcgtcgg tcggaaggcc gagcgcgtgc
     1981 cggaggatca cctcctccag cgagatgccc gccccgaagc ggagggagcg ggagcagagg
     2041 ccgccgatcg agcgggccgc cacctcgagc acgacgggcc cggacggccc gagcctcacc
     2101 tcggcgtgga tcgggccctc gcgcagcccc atcgccttcg cggcgccggc cgcgagggtt
     2161 cgcaccgagt cctgcatctc gaacggctcc cgcgagggcg tgacgtagat cgtctcctcg
     2221 aaggtcggcc cctccatcgg ctcgggcttg tcgaacaggg ccagggtctc gagatcgccc
     2281 cggaggagca ggccctccag ggccatctcc tggccgggga cgtaccgctc caccagcagc
     2341 ggaccgagag gatcctcccg ggcccgggcc aggatctccc ggatgtgccc ggcggcggcc
     2401 cgggcctccc gcgggctgtc ggcccggagg acgccgcggc tcatcgagag cgacaccggc
     2461 ttgaggacga ccgggtagcc cagctcgtcc gccaggtccc ccgcggcgtc ctccgggccg
     2521 aggacccgga acccgggctg ggggacgccg gccgcggcca gagcccggcg catcctgagc
     2581 ttttcggtgg cggcggtggc cgccccctgc gggttgtggg gcagatccag ccgctcggcg
     2641 gccaccgccg ccaccaccac cccgcggtcg tccaccggca cgaccgcgtc gatcgcgtgc
     2701 tccctgccga accgctcgat ccgcttggcg gctgcgtccg ggtccgacat cggaacggtc
     2761 agcgcccggt cctccatctg cgcggcgagg gcggggcggc ggtcggaggc cacgacgacc
     2821 tcgaccccga gcgcctcggc ggcctcgagg aagtcgtccg cgcggtaggc cgtcgcgggc
     2881 aggagcaaga ggatccgggc cacgcctgta tcctaggcag tgatgagcga acagccgaag
     2941 gacgagctcc tcacgatcac cgcgcaggct cgagaggccc tgctcggcct gcgggcccag
     3001 gaccctgccc cggactcgct cggcctgcgg atcgcggtgg cgggggagcg ggacgggcgc
     3061 tacgtgcacg acatgtcgtt cgagcttctt gaggacgcgg gcccgggcga cgtcgtcctc
     3121 cagcacgacg acatcccgat gatcgtcccc caggactcga tgaagaagct gcgcggcgcg
     3181 gtcgtcgacc tcgcccccga cggtcagtcc tggacgatcg acaacccgaa ccggcccaag
     3241 cggatgctgc cgctggctcc cttgggtggg gggcatgccg gccacgacca cgcggggcac
     3301 gagcaggccg cgagccccgc ggcccaggcc gagatccccg aggagctgcg cggcaccctc
     3361 gagggggaca ccgcccagca ggtgatcgag gtcctcgacc gccacatcaa ccccagcatc
     3421 gcgatgcacg gaggccacgc ggagctggcc ggcctcaagg agggcgtggc atacctccgc
     3481 ctctccggcg gctgccaggg ctgctccatg gcggcggtca ccctgcgtca ggggatcgag
     3541 cgctccctcc gccagctcgt ccccgagatc cagggtgtgg ccgacgtcac cgaccacgcc
     3601 tcgggcaccg accccttcta cagctcctcg aagggctagc taggcgccgg ggagccgcga
     3661 ggcgaacatc ttccggggct cgatccggaa gtaggggggc ggggcctcgc cccaccacgt
     3721 ctcccagtcc gggtacacct cgagcaggta cgaacggaac ccctcgtgct ctgccgccgt
     3781 cacgtcggcc gggcgggcct caccgtgcac cgcaatcacg agctcctcgc ccctcgcgtg
     3841 caccgcgctc acctgtggcc gcctgctcag gtgattgaag cggaccgacc cggccgacgt
     3901 cccgaagtgg aacgcccccc ggtagaagag tccgtccacc ggccccgcga tcgggcggca
     3961 gtcggacgtc acggtcgaca ggaccatcac ctggacgccg ggcagcagct cgggcagctc
     4021 gtcggcgctg atcctgcgct cgtcctggag gatgctccga aggtggccgc cgccgccggc
     4081 gtggctctcg tccaggacct tcccgagccg ctcgagatcc tccggggtct cgtgcacgga
     4141 cggctactcc tcggcgccct cggccttcag ctcctggacc agccggtccg cgatgtgcga
     4201 ggggacctcc tcgtagtgcg agaacgtcat ggagaaggtc ccgtgccccc cggtgatgga
     4261 ccgcaggtcg atggggtagc gcgtcatctc tgtgagcggg acctgagccc ggatcctcgt
     4321 cttcccggtg ccggtggggt ccatcccctg gaccttcccc cgcttcgagt tgaggtcgcc
     4381 catgatgtcg ccggtcatcc cctcggggac gagcacctcg acgtccacga tgggctcgag
     4441 caacacgacc cccgcgttct gggtcgcgtc ccgaaacgcg agcgccccgg cgatctggaa
     4501 cgccatgtcc gaggagtcga ccgagtggta cttcccgtcg tagagggtga ccttcacgtc
     4561 cacgaccggg aaccccgcca ggaccccttc ctgcatcgcc ttgacgatgc ccttctccac
     4621 cgatccgatg aactggttcg ggagcgcgcc gccgaagatc tcgtcctcga agacgaagcc
     4681 ctcacccctc gtgagtggct cgatgcggat gttgcacacg ccgtactggc cgtgcccgcc
     4741 ggactgcttc acgtgtcggc ccaggccctc ggccttcccc cggatcgtct ccttgaacgc
     4801 gaccttggcc ggctcggtgg tcacctccac cccgaacttc cggtgcatgc gctcgatcag
     4861 cacgtcgatg tgggcttcgc ccatcccggt gacgacggtc tggccggtct cgacgcgacg
     4921 ctcgacgcgg atcgtggggt cctcgtcctg gatccgctgg agcgcggtgg agagcttgtc
     4981 ctcgtcgccc tgggtcttcg ggtggacggc caccgcgtag aggggctctg cccactccac
     5041 cgcggggagg gtcgccgccg cctccttcgt cgtcagcgtg tcgccggtcg ccgtgtccga
     5101 gagcttcgac accgcggcga tgtccccggc gggcacgtcg gagacgttct cgtgctcctt
     5161 acccctcagg gtgaacacct ggtggatccg ctcgtcggag cccttcgtcg cgttgtgcac
     5221 ggtcgagtcg ggccgcatcc ggcccgacag gacccggaag agcgagatct ggcccacgta
     5281 ggggtccgag aagctcttga agacgaaggc ggtgaggggc ccgttcgggt cggcctcgcg
     5341 ctcctcctcc tcgcctgcct tcgtcatcac cttgacgggc ggccgatccg tgggggcggg
     5401 gaaggcgtcc acgatgaggt cggcgaggcg gtcgacgccc ttggcggagg ccgccgagcc
     5461 gaccaggacc ggcacgaagc gcgcctggag aagtcccgtc ttcacgcccg aggtgatctc
     5521 ctcggcgctc agctcgccct cctccaggta cttctccagc atcgcgtcgt cgccctcggc
     5581 cacggcctcg gtcagcttct cgcgcatcgg ctcggccttc cccgcgatgt cgtcgggcca
     5641 gtcgctctcc tcgctctgcg gcccgctcgg gtaccgatac gccctgccgc tcaggagatc
     5701 cgccacgccc tcgaagtcgt gctcctcgcc gatggggaac tgcagggggg ccacctgggt
     5761 cccgaacgcc tgcaccagct gatccagcgt ccgctcgaac gaggcgcgct cccggtcgat
     5821 cttgttgacg aagacggccc gcggcagccc cgcctccacg gccatctccc agacgacctc
     5881 gtgctggacc tggacgccgt ccaccgccga gaccacgaac aggcaggcat cggccgcccg
     5941 gagcgcggcc tgcacgtccc cgatgaagtc ggcgtagccg ggggcgtcga ggaggttgat
     6001 cttcgtggcc ctccagtcga agggggccag cgccagcgag acgctgatct gcttcttctg
     6061 ctcctcggcc tcgaagtcgg tgacggtgtt gccctcctcc acggtgccca tccgggtggt
     6121 ggctccggcg gcgaagagga gggcgtcggc gagggtcgtc ttcccggtgc ccccgtgtcc
     6181 gacgagcagg acgttgcgga tcgcgttcgg ttcgtaggta ttcatcggca cctcatgggg
     6241 tcggcgttcc ccaaattata ggcggggccc cgagagggcg ttccggccgc cggggccccg
     6301 gggacgccgt gctacgctcg catcccccat ggagcagacc atccgacccc gcccctcgtc
     6361 ggtgtggacg atcctcgtcg gcgcggtcct ggcggcggcc tcgtcggcgg tcttcgtgct
     6421 cgtcgatgag ctccagatgg ccggcgcggc ggcggcggtc gccgtgctcg gcgtggtggt
     6481 ggccgggtgg agtgcccggg agtcgggcga gccccgcgtg gggttcgcgg cctccatgct
     6541 ggaacggatc gtggacgcgg ccatcctggg agcggtggcc tggtcgctgc tgccggagcg
     6601 ggccggggcc gcggcgctcg tggccctggc cggctcctac gtggccgcgt acctcgaggt
     6661 gaaggcatcc gggctcgggt tcgaggcggg cgcctggggc ctggcctggc ccctggcgat
     6721 ggtcgtcctc gccgcgggcc tggtcgccgg ggaggccgag ctcggcctgt ggggggctgc
     6781 ggcgatctcg atcgccgccg ccctggccag gtcggtccag gtcgcccgtc agcgggagcc
     6841 gtcgtgaccg agcgggagac gttcgccgag accttctcgt actggtacta ccgcagcgcc
     6901 gcgtgggcgg cccgggcgct gcccgagcgc gtcgggcggc gtctgttctc ggccgcggcg
     6961 gccgtcgagc acgcccgcaa ccatgcccag cggcgcgtcg tctcccggaa cctgtctcgg
     7021 gtgctgggca agcccccgga cagcctggag gtgcgggccg cggtccgcga ggcgttcgac
     7081 tcctatggcc gctactggta cgacaccttc ctcctgcggg ctctggctcc cgagctggtc
     7141 cgcaagcgct tcgacatgcg agggctggag ttcgtggacg ccgccctcga gcaggggagg
     7201 ggggcgctct tctgcctccc gcatctcggg aactgggacg tggcggggca ctggatgacg
     7261 ctgcagggct acgcgctgac ggcggtggcc gagacgctgc ggcccgagcg cctgttccac
     7321 ctgttcctcg agcatcgccg ggacctcggg atgaacatcg tgccgctgac cggcacc
//
LOCUS       DASUTW010000050         2002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_533669, whole genome shotgun sequence.
ACCESSION   DASUTW010000050 DASUTW010000000
VERSION     DASUTW010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533669"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VF058_01780"
     CDS             <1..727
                     /locus_tag="VF058_01780"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6207065.1"
                     /translation="VLAERLKEDGVEIRTDVTAERVERDGPGRVVHLTDGSTAVGSEL
                     LIAIGRRPTDLRELGLEEAGIELDDRGAPPTHDDTMRIGDGVLVAGDAAGGPQFTHVA
                     VYHGRVAAMTALGREARADLTAVPKAVFTDPEIASVGLTVEEARDEGIDAFEETTDFA
                     ATTRGFLTEGSTGHLTVVVDRERRQVLGAFAAAPAASELIHLPALAIKLGTPLDVLVD
                     NITAFPTGARELTHVFDAALKKL"
     gene            741..1121
                     /locus_tag="VF058_01785"
     CDS             741..1121
                     /locus_tag="VF058_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6207066.1"
                     /translation="MFKPRSVHPTIPVRDLEAAKAWYAEKLGIEPDRDDFDGSWFKIG
                     DRDLLLYPSGFAGTNQATTAEFLVDDVPEAVADLRERGVVFEEYDFPELKTVEGIATF
                     GDQQTAWFKDPDGNILALTSLPQS"
     gene            complement(1125..1667)
                     /locus_tag="VF058_01790"
     CDS             complement(1125..1667)
                     /locus_tag="VF058_01790"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX6207067.1"
                     /translation="MATLVTPRRALALAFLLVVGGVLVALVASADRASPTDPIEVSGP
                     MPDVEEPSINGMGEVTPSLYRGKVVLVNFWASWCGPCRREQPGLSRLWEEYRDRGVQF
                     LGVDFRDDRAAGLAYLDEFDVPYPSVEDPTGIIAHRFGVPYLPATILVDRAGEMRLRL
                     VGAQTEEALRVHLEELLSEA"
     gene            complement(1696..>2002)
                     /gene="rnpB"
                     /locus_tag="VF058_01795"
     ncRNA           complement(1696..>2002)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VF058_01795"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          304 a          715 c          714 g          269 t
ORIGIN      
        1 cgtgctggcg gagcgcctga aggaggacgg ggtcgagatc cggacggacg tcacggccga
       61 gcgggtggag cgcgacggac cggggcgggt cgtgcacctc accgacggat ccacagccgt
      121 gggctcggag ctcctgatcg cgatcgggcg gcggccgacc gacctccgag agctcggcct
      181 ggaggaggcg gggatcgagc tcgacgaccg aggggctccc ccgacccacg acgacacgat
      241 gcggatcggc gacggcgtgc tcgtggccgg cgacgcggcg ggcggaccgc agttcacgca
      301 cgtggccgtc taccacgggc gggtggcggc catgactgcg ctgggtcggg aggcccgggc
      361 cgacctgacc gcggtgccca aggccgtctt caccgacccc gagatcgcct cggtggggct
      421 cacggtggag gaggctcggg acgagggcat cgacgccttc gaggagacga ccgacttcgc
      481 cgcgacgacc cgcggctttc tgaccgaggg ctcgacgggc cacctgacgg tggtggtgga
      541 tcgcgagcgc cggcaggtgc tgggggcctt cgcggccgcc cctgccgcct ccgagctcat
      601 ccacctgccg gcgctcgcga tcaagctcgg cacccccctc gacgtcctcg tggacaacat
      661 cacggccttc cccaccggcg cccgcgagct cacgcacgtc ttcgacgccg cgctgaagaa
      721 gctctgagag aggagaactg atgttcaagc cacggtcggt gcacccgacg atcccggtgc
      781 gggacctcga ggccgcgaag gcctggtacg cggagaagct cggcatcgag cccgaccgcg
      841 atgacttcga cggctcctgg ttcaagatcg gcgaccggga cctcctgctc taccccagcg
      901 ggttcgccgg caccaaccag gccacgaccg ccgagttcct ggtggacgac gtccccgagg
      961 cggtcgccga cctccgcgag cggggcgtgg tcttcgagga gtacgacttc ccggagctga
     1021 agaccgtgga gggcatcgcc acgttcgggg atcagcagac cgcctggttc aaggaccccg
     1081 acggcaacat cctggccctg acgagcctcc cgcaaagctg aggctcaggc ctcggacagc
     1141 agctcctcca ggtggacgcg gagtgcctcc tcggtctgag cccccacgag ccgaaggcgc
     1201 atctcccccg cccggtccac gaggatcgtc gcggggaggt acggcacgcc gaagcggtgg
     1261 gcgatgatcc ccgtgggatc ctccaccgag gggtagggca cgtcgaactc atcgaggtag
     1321 gcgaggccgg ccgcccggtc gtcgcggaag tccacgccga ggaactggac gccccggtcc
     1381 cggtactcct cccacaggcg cgagagcccc ggctgctccc ggcggcacgg gccgcaccac
     1441 gaagcccaga agttcacgag cacgaccttc ccccggtaga gcgagggcgt gacctccccc
     1501 atcccgttga tcgagggctc ctcgacgtcg ggcatcgggc ccgagacctc gatcgggtcc
     1561 gtgggcgagg cgcggtcggc gctcgccacg agcgccacga gcacgcctcc caccacgaga
     1621 aggaacgcga gggccagggc ccgccgaggg gtcaccaggg tcgccacgag gaccaacggt
     1681 agcgcagagg tggtgccgag gagcgggcct gtaagccgga ttctgtgtcc cccgaagggg
     1741 cggcgaccat ctctctggga gccgtgtcgc cacggccctc ggcgcggcct acccgggagc
     1801 atcggacggg ccgtccgctc cctgcttggc cttgctccgg gtggggtttg ccgagccgcg
     1861 acggtcgccc gccgcgctgg tgagctctta cctcaccgtt tcacccttac ctcccgaggg
     1921 aggcggtctg ctctctgtgg cactttccgc gggtcgcccc gcctgggtgt tacccagcac
     1981 cctgccctgt ggagtccgga ct
//
LOCUS       DASUTW010000051         2646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1829586, whole genome shotgun sequence.
ACCESSION   DASUTW010000051 DASUTW010000000
VERSION     DASUTW010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829586"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VF058_01800"
     CDS             <1..411
                     /locus_tag="VF058_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018521251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6207068.1"
                     /translation="GRFIMPWIVISLSFAAFYARMVRGNLIETMGEDYMRTARAKGLS
                     ERRVILRHGLRSALTPVVTMFGMDMGILLGGAIITETVFNLPGIGQFAIRSLVTNDIP
                     AVMGVTMLAAFFIVVANLVVDIVYAFLDPRVRYT"
     gene            445..1458
                     /locus_tag="VF058_01805"
     CDS             445..1458
                     /locus_tag="VF058_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007266703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6207069.1"
                     /translation="MAEPLLEVRDLRVHFPTEDGVVKAVDGVSFTVEPGETLGVVGES
                     GSGKSVTFLTVLGLVGRREADISGQILFRGKDLLQTPEDELREFRGSKASMIFQDPMT
                     SLHPYYKVGRQIVEAIVTHEKISKREAMDQAVEMLRRVGIPRPEERARQYPHEYSGGM
                     RQRAMIAMALALNPDLLIADEPTTALDVTVQAQILDLIDRLKEEFGAAVVIITHDLGV
                     VAEHCDNIMVMYAGKPAEYGTRQDIYYDAHHPYTWGLLGSLTRLDQPRSERLRSIPGL
                     PPSLIQVPPGCPFHPRCPYVMDVCKVEVPRLLADESGSHRSACHLPLAEKERIFQEEV
                     AVT"
     gene            1488..2519
                     /locus_tag="VF058_01810"
     CDS             1488..2519
                     /locus_tag="VF058_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX6207070.1"
                     /translation="MAPEPDSSRTPLVKVVGLKKHFPITRGVIFQRKVGAVHAVDGID
                     LEVYPGETVGLVGETGCGKSTTARLIMKLLEATDGQVFYDGQDITRYGRRRMRELRGE
                     MQMIFQDPFASLNPRKTVGSIIGEPFRVHGTTPKAGIKREVQDLMELVGLNPEHYNRY
                     PHEFSGGQRQRIGVARALALRPRLIVADEPVSALDVSIQAQILNLLEDLQEEFNLTYL
                     FIAHDLSVVKHMSDRVAVMYLGKIVEIAPGDTLYRNPKHPYTGALLSAVPIPDPKAAE
                     LKRRIVIEGDVPSPIDPPSGCRFHPRCPKAQFPTCSDIEPPLEPHHRGQLAACHFPLE
                     DHAVVESSA"
BASE COUNT          427 a          937 c          867 g          415 t
ORIGIN      
        1 ggccggttca tcatgccgtg gatcgtgatc tcgctctcgt tcgcggcgtt ctacgccagg
       61 atggtccgcg ggaacctcat cgagacgatg ggggaggact acatgaggac ggcgcgggcc
      121 aaggggctct ccgagcgacg ggtgatcctg cggcacggcc tgcgctccgc cctgaccccg
      181 gtcgtcacga tgttcgggat ggacatgggg atcctgctcg ggggggccat catcaccgag
      241 accgtgttca accttcccgg catcgggcag ttcgcgatcc gatcgctggt cacgaacgac
      301 atccccgcgg tgatgggcgt gacgatgctg gccgcgttct tcatcgtcgt ggccaacctc
      361 gtggtggaca tcgtctacgc gttcctggac ccccgcgtga ggtataccta gaccccgtgg
      421 acgacgatcg agagaggggg ccgggtggct gagccgctgc tcgaggtgag ggacctgcgg
      481 gttcacttcc cgaccgagga cggcgtcgtg aaggccgtgg acggcgtctc cttcacggtg
      541 gagcccgggg agaccctcgg cgtggtgggc gagtccggct ccggcaagag cgtcacgttc
      601 ctcaccgtcc tgggcctggt cggccggcgg gaggccgaca tctcggggca gatcctcttc
      661 cggggcaagg atctccttca gacccccgaa gacgagctgc gagagttccg cgggagcaag
      721 gccagcatga tcttccagga ccccatgacc tcgctccatc cctactacaa ggtgggcagg
      781 cagatcgtcg aggccatcgt cacgcatgag aagatctcga agcgggaggc gatggaccag
      841 gccgtcgaga tgctccggcg cgtgggcatc ccccgccccg aggagcgggc gcgccagtac
      901 ccccacgagt actcgggagg gatgcgccag cgggccatga tcgccatggc gctcgccctc
      961 aaccccgacc tcctgatcgc ggacgagccc acgaccgcgc tcgacgtgac ggtccaggcg
     1021 cagatcctcg acctgatcga ccgcctcaag gaggagttcg gcgcggccgt cgtgatcatc
     1081 acgcacgacc tcggcgtcgt cgcagagcac tgcgacaaca tcatggtgat gtacgccggc
     1141 aagcccgccg agtacgggac gcgccaggac atctactacg acgcgcacca cccctacacg
     1201 tggggcctgc tgggatcgct cacccgcctc gaccagcccc gcagcgaacg cctccggtcg
     1261 atccccggtc tcccgccctc gctgatccag gtccccccgg gctgcccgtt ccacccgagg
     1321 tgcccctacg tcatggacgt ctgcaaggtc gaggtgcccc ggctcctcgc cgacgagtcg
     1381 ggctcgcacc gctcggcctg tcacctgccg ctcgcggaga aggagcgcat cttccaggag
     1441 gaggtggccg tcacgtgacc gaggcgatcc ccgcgccgca ggctcccatg gctcccgagc
     1501 ccgactcctc gcggacgccg ctggtcaagg tggtcggcct gaagaagcac ttcccgatca
     1561 cgcgaggcgt catcttccag cggaaggtgg gcgccgtcca cgccgtggac gggatcgacc
     1621 tcgaggtcta tcccggcgag acggtcggcc tggtgggtga gaccggctgc ggcaagtcca
     1681 ccacggcccg gctcatcatg aagctcctgg aggccaccga cggacaggtc ttctacgacg
     1741 ggcaggacat cacgaggtat gggcgccgcc ggatgcggga gctccgcggc gagatgcaga
     1801 tgatcttcca ggatcccttc gcctcgctca atccccggaa gaccgtggga tcgatcatcg
     1861 gcgagccgtt ccgcgtccac ggcaccacgc ccaaggccgg gatcaagcga gaggtccagg
     1921 acctgatgga gctcgtcggc ctcaacccag agcactacaa ccgctacccg cacgagttct
     1981 cgggcggcca gcgccagcgg atcggcgtgg cccgcgccct cgccctccgg ccgcgcctga
     2041 tcgtggccga cgagcccgtc tcggccctcg acgtctcgat ccaggcgcag atcctcaacc
     2101 tcctggagga cctccaggag gagttcaacc tcacctatct gttcatcgcc cacgacctct
     2161 cggtcgtgaa gcacatgagc gacagggtcg cggtcatgta cctggggaag atcgtcgaga
     2221 tcgcgccggg agacactctg taccggaacc cgaagcaccc gtacacgggc gcgctgctgt
     2281 cggccgtccc gatcccggac ccgaaggcgg cggagctgaa gcgacgcatc gtgatcgagg
     2341 gcgacgtgcc gtccccgatc gaccctccgt cggggtgccg gttccatccc cggtgtccga
     2401 aggcccagtt cccgacctgc tcggacatcg agccgcccct cgagccccac caccgcggcc
     2461 agctcgccgc gtgccacttc ccgctcgagg accacgccgt ggtagagtcg tcggcctgat
     2521 gctcgacgcg atcctgatcg tcatcaacgt gctctcggcc ctgctgctga tcgtgttcat
     2581 cctgctccac gcgggacggg gaggcgggct ctccgacatg ttcggcggcg gcggcgccgc
     2641 gctctc
//
LOCUS       DASUTW010000052         3588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_923297, whole genome shotgun sequence.
ACCESSION   DASUTW010000052 DASUTW010000000
VERSION     DASUTW010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923297"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VF058_01815"
     CDS             complement(<1..185)
                     /locus_tag="VF058_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207071.1"
                     /translation="MREARPFEPFPEEVRFLEAAFFVGGVGAALLPPDTRWGRRAAPA
                     PPTPSPSAGARYANSPA"
     gene            complement(190..1038)
                     /locus_tag="VF058_01820"
     CDS             complement(190..1038)
                     /locus_tag="VF058_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207072.1"
                     /translation="MGRATDLSAVLEGRAYAELLGWRIVEVSGADARAWLHDLLMADV
                     ASLSPARARRALMLKPTGHIRADLTVASRGDGFLLVQDPRQPTPIDLLLAPYVLSADV
                     RLEDRSDRLRAVAFPERPAEGADAYAPSASGPGHDVILAREAVVEARGAQEVGLEAIE
                     VWRIARGEARFPQDLGDRSLPNEAGLEALIEDAEKGCFLGQEAVAKTRTLGRPPFAVI
                     RARAGEPVEEGQAVRAGDDEVGEVTSAAEVDGGWALIARVRWSSREGPLTVGDRAIEV
                     RGLATG"
     gene            1059..2207
                     /locus_tag="VF058_01825"
     CDS             1059..2207
                     /locus_tag="VF058_01825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6207073.1"
                     /translation="MDAAADRPQDLLPRILVVTNDFPPRVGGTQRYVHDLVRNLPPDR
                     VTVLAPPWEGWEAFDEAQPFPVLRHRRMLVPTPELRRRVLSLLDETGAEIVLFGHGYP
                     LGELGPGLKAATGVPYAMLTHGLEFMLAAMPVVRSRMRRAFSEARVVFTVSRFMQHKL
                     RRAIPWQVERMPLPPGVDLERFHPGVSGLEVRARHGLSDRLVVACISRLVPRKGQDVL
                     LRAWPRVLRHVPDAALLLVGDGPHRGALARLRSRVDGTVIFAGEVPEEELPAHYAACD
                     VFAMPCRDERFGFEVEGLGIVYLEAAATGRPVIAGRSGGAPEAVLDGETGFVVDGADV
                     SDVTEALVALLRDPSEARRLGKAARARAEREWGWDVVIRRFASAIAPR"
     gene            complement(2204..3184)
                     /locus_tag="VF058_01830"
     CDS             complement(2204..3184)
                     /locus_tag="VF058_01830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6207074.1"
                     /translation="MSLEWIADPGVFELDDWERLRAADPEATFFHTPSFLRIYREQLG
                     EGTPLVGLVRRDDRAIGLSPFEISGGLLSFLGGFEVTDYLGPVGRPEDRARVAEELMT
                     GLAARQDWDRGDLEGLPEDGTWLAALADAADAAGLRTEIGDDDVAPRLELPSTWDEYL
                     AGLKPKQRHEIRRKDRRFRERFAEVRLRASTPDTLDRDLDRFVEMHRASEGPKGTFME
                     EAMVGFFRRLGRELLPTGVFRLTFLEGDGEAVAGLASFRFEGQVLLYNSAFDPRHRGV
                     APGMVLVAEVIREAIAEGCARLDMLKGDLGYKYRFGARPRAIRRLRLERS"
     gene            complement(3216..>3588)
                     /locus_tag="VF058_01835"
     CDS             complement(3216..>3588)
                     /locus_tag="VF058_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207075.1"
                     /translation="PDPVRVPADDDEFEAVLLLFVLHHMPAWEDQVRLLADAARAASS
                     RLIVIEDTPLHRIDRAFNVAWDWLLNLRHGVPKPFTFRDVEGWRRVFADLELRERHAE
                     TYRPLWPTLGTYHHTLFVLET"
BASE COUNT          465 a         1330 c         1311 g          482 t
ORIGIN      
        1 ctggccggag agttggcgta ccgggcgccg gccgacggcg acggggtggg gggcgccggc
       61 gcggccctcc ggccccagcg agtgtcgggc gggagcaggg ccgcgccgac gccccccacg
      121 aagaaggcgg cctccaggaa gcggacctcc tccgggaagg gctcgaaggg ccgagcctcc
      181 cgcaagcggt cagccggtcg ctaggccccg aacctcgatg gcgcggtcgc cgacggtgag
      241 cggcccctcg cggctcgacc aacgcacccg cgcgatcagg gcccatccgc cgtctacctc
      301 cgcggcgctc gtgacctcgc ccacctcgtc gtcaccggct cggaccgcct gcccctcctc
      361 cacgggctcg cccgctcggg cccggatcac ggcgaacggc ggtctcccca gcgttcgcgt
      421 cttggccacc gcctcctggc ccaggaagca gcccttctcg gcgtcctcga tcagggcctc
      481 cagcccggcc tcgttgggga gggaccggtc cccgaggtcc tgcgggaagc gtgcctcgcc
      541 ccgggcgatc cgccacacct cgatcgcctc gagcccgacc tcctgcgctc ctcgcgcttc
      601 gacgactgcc tcgcgggcga ggatcacgtc gtggcccggg ccgctggccg agggagcgta
      661 ggcgtccgca ccctccgccg gacgctccgg gaaggccacg gcccggagcc tgtcgctccg
      721 gtcctcgaga cggacgtcgg cggagagcac gtacggggcg aggaggaggt cgatcggcgt
      781 gggttggcga gggtcctgga cgaggaggaa gccgtcgccg cgggacgcca ccgtcaggtc
      841 ggcccggatg tggccggtgg gcttgagcat cagcgcccgg cgggcccggg cggggctcag
      901 cgaggccacg tcggccatca gcaggtcgtg gagccacgcc cgagcatcgg cccccgagac
      961 ctccacgatg cgccagccca gcagctccgc gtacgcccgc ccctcgagca cggcggacag
     1021 gtcggtcgct cgtcccatgg gtgcaggata atcaccgggt ggacgccgcc gccgaccgcc
     1081 ctcaggatct cctcccacgg atcctcgtcg tgaccaacga cttccctccc cgcgtgggcg
     1141 ggacccagcg gtacgtccac gacctcgtgc ggaacctccc acccgaccgg gtgaccgtcc
     1201 tggccccccc gtgggagggg tgggaggcct tcgacgaggc gcagcccttc cccgtcctgc
     1261 gtcaccggag gatgctggtc cccacgcccg agctccgccg ccgcgtcctc tcgctccttg
     1321 acgagaccgg agccgagatc gtgctgttcg gccacggcta cccgctgggc gagctgggtc
     1381 ccggcctcaa ggcggccacg ggcgtgccct acgccatgct gacccacggc ctcgagttca
     1441 tgctcgcggc catgcccgtg gtgcgctcgc ggatgcggcg tgccttctcg gaggcgcggg
     1501 tggtcttcac cgtcagccgg ttcatgcagc acaagctccg tcgggccatc ccgtggcagg
     1561 tggagcgcat gccgctcccc ccgggggtgg acctggagcg cttccacccc ggggtgagcg
     1621 ggctcgaagt gcgagcgcgc cacggtctga gcgaccgcct cgtggtggcg tgcatctccc
     1681 ggctcgtccc ccggaagggg caggacgtgc tgctccgggc ctggccccgc gtgcttcggc
     1741 acgtccccga cgccgcgctc ctgctcgtgg gcgacggacc gcatcgaggg gcgctggccc
     1801 gcctccggag cagggtggac gggacggtga tcttcgcggg ggaggtgccc gaggaagagc
     1861 tcccggccca ctacgccgcc tgcgacgtct tcgccatgcc ctgccgcgac gagcgcttcg
     1921 gcttcgaggt ggaggggctg ggcatcgtgt acctggaggc cgccgcgacc gggcgtcccg
     1981 tgatcgccgg gcgatcgggc ggcgcgccgg aggcggtcct cgacggggag accggcttcg
     2041 tcgtcgacgg tgccgacgtc tcggacgtca ccgaggcgct cgtggccctc ctccgggatc
     2101 cgtcggaggc ccgcaggctg ggcaaggccg cccgggcccg ggccgagcgg gagtggggct
     2161 gggacgtggt gatccggcgg ttcgcgagcg cgatcgcgcc tcgctagcta cgctcgaggc
     2221 ggagcctccg gatggccctg ggccgcgcgc cgaaccggta cttgtagccg aggtccccct
     2281 tcagcatgtc gaggcgggcg cagccctcgg cgatcgcctc ccggatgacc tccgccacga
     2341 gcaccatgcc gggcgcgacc ccgcggtgcc ggggatcgaa ggcggagttg tagaggagca
     2401 cctggccctc gaaccggaag gacgccaggc cggccacggc ctcgccgtcc ccctcgagga
     2461 acgtgagccg gaacacgccg gtgggcagca gctctcgtcc gaggcggcgg aagaatccca
     2521 ccatcgcctc ctccatgaag gtccccttcg gcccctcgct ggcccggtgc atctccacga
     2581 accgatcgag gtcacggtcc agggtatcgg gggtggaggc ccggagccgg acctcggcga
     2641 accgctcgcg gaagcgtcgg tccttgcgcc ggatctcgtg ccgctgcttg ggcttgagcc
     2701 cggccaggta ctcgtcccag gtcgagggga gctcgagcct cggggccacg tcgtcgtccc
     2761 cgatctcggt ccgcagcccg gccgcgtccg ccgcgtcggc cagcgctgcc agccacgtgc
     2821 cgtcctcggg caggccctcc aggtcgcctc ggtcccagtc ctggcgggcg gcgagcccgg
     2881 tcatcagctc ctcggccacc cgggcccggt cctcgggccg gcccacgggc cccaaatagt
     2941 cggtcacctc gaacccgccc aggaacgaga gcaggccgcc ggagatctcg aagggggaga
     3001 ggccgatcgc ccggtcgtcc cgacggacca gccccacgag gggcgtcccc tcgcccagct
     3061 gctcccggta gatcctcagg aacgagggcg tgtggaagaa ggtggcctcg gggtcggccg
     3121 cgcggagccg ctcccagtcg tccagctcga agacgcctgg atcggcgatc cactcgaggg
     3181 acacgtcggg catcgtacca acgacccctc gggcctcagg tctcgagcac gaagagcgtg
     3241 tggtggtagg tgccgagggt gggccagagg gggcggtagg tctccgcgtg gcgctctcgc
     3301 agctccaggt cggcgaagac ccgtctccac ccctcgacgt cgcggaacgt gaagggcttg
     3361 gggacgccgt gccgcaggtt cagcagccag tcccaggcca cgttgaaggc gcggtcgatc
     3421 cggtgcagcg gcgtgtcctc gatcacgatg agccggctgc tcgccgcgcg tgccgcgtcg
     3481 gcgagcagcc ggacctggtc ctcccatgcg ggcatgtggt ggaggacgaa gagcagaagg
     3541 acggcctcga actcgtcgtc gtcggccggg acgcggaccg ggtcgggg
//
LOCUS       DASUTW010000053        10067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_792719, whole genome shotgun sequence.
ACCESSION   DASUTW010000053 DASUTW010000000
VERSION     DASUTW010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10067
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792719"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3032
                     /locus_tag="VF058_01840"
     CDS             <1..3032
                     /locus_tag="VF058_01840"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6207076.1"
                     /translation="SLRRVMTRYFEAMKVILERHGGLVEKFIGDAIVAVFGAPVAHED
                     DAHRAVRAAVVMRDALRTLNEEFRRAWGVTVATRTGLNTGEVVTGDPKTGGTLVTGDT
                     VNVAARLEQAAEPGEILLSEATYRLVRNVAPTEAVGPFAIKGKATPVRARRLLDVGAE
                     APPRGFLGLDSPIVGRDRELEQLRAAFQRTNEARRCEVVTVLGAPGVGKSRLTAEFLA
                     ELPVDVRVVSGRCLPYGEGITFWPIVGVLRESAGVTETDSPEQADAKFATLLEGVADA
                     GLIRQRLTALLGISDVAPGIQETFWAVRKLFEELARRRPLVVVFDDIHWGEPTFLDLL
                     EYLVDWLQGVPVLLVCVARPELLEGRGEWLAGKDNVVLIKLGPLAEPYTTRLIQTLLG
                     NVPLPEEASTSIADVTEGNPLFVEETLRMLIDEGSLRRRNGVWSVDEDLSALSIPPTI
                     QSLLTARVDMLAAGEREVIERASVVGRVFWWGAVSGLSPREDQAHVAGWLASLVRKEL
                     IRPERSDLMEEDAYRFTHILVRDAAYEGVPKARRAELHERFARWLHEKSRDRAGEYEE
                     IVGYHLEQAYRLLRELGPATERTWAMGLAAAAPLASAGKRALARGDMPAAANLLSRAA
                     ALLPDDDRNRMDLLPDLAFALLQTGGFGPTREVLAEIQRAAAISEDVLLGARGQLLDL
                     AVRMFTNPEGWAEESSREATRAISVFGEHKDERGVAQAWALLGLSHMIAGHFATAVEA
                     WEEAAQHATAAGAHREGLEHLAWIPVGVWSGPMPVEEAIGRCREIGARAHGDRKATAV
                     ALAVQGNLEAMRERYAEARELGVRARSVLQEVALPGWLGALTQMSGWTEILAGDPVAA
                     EGELRSGVEMLRGIGEQSWLSTTAAMLAEALFAQDRLDEAEELVRLSEETAGSEDIFS
                     QVLMRTVRAKVRARQDRPDEAAGRAEEAVAIARSSDSLLLQGEALTSLGYVMRVAGRE
                     AEGDAALVNAVRVCEQKGNLAGAARARAVRASSQPAP"
     gene            3372..3719
                     /locus_tag="VF058_01845"
     CDS             3372..3719
                     /locus_tag="VF058_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207077.1"
                     /translation="MLNRAALILRYKQPFVDWINAADPSPTSHTLTVDEVNQEHTVYL
                     VEVEDEDEPAEWLVRHHEELFEAELNGWYTDPALWPGTDRSRCSRSGVRSSSTPSWWT
                     TGASPLEDDELES"
     gene            3809..3956
                     /locus_tag="VF058_01850"
                     /pseudo
     CDS             3809..3956
                     /locus_tag="VF058_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606173.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4010..4747)
                     /gene="bshB1"
                     /locus_tag="VF058_01855"
     CDS             complement(4010..4747)
                     /gene="bshB1"
                     /locus_tag="VF058_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HEX6207078.1"
                     /translation="MNTADVLAIGAHPDDVEVGCGGTIILAADADLDVVVATLTSGET
                     ASRGTLDDREHEGERAAEILGATTYPSLGLPDGRVGVDPAHRTALLELIRELEPRIVL
                     APYEQDRHPDHAAAGRLVRETSFLAGLPRLGEGTPHRPAHVYHYMVHHPFPPSFVVDV
                     SRVWDRKWRAVRAHESQFGPEGGSRGPVDPGRFLQVVEAWATVHGAMIGVERGEAFHS
                     SGPVPVTSLPGLARLEGEAPSRYQIFH"
     gene            complement(4747..5901)
                     /locus_tag="VF058_01860"
     CDS             complement(4747..5901)
                     /locus_tag="VF058_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207079.1"
                     /translation="MRSLNDLLASAEGRFFLESRGVILQEAGFGRELAPPAYRGLAEA
                     LGLPDGARPVYVAHQTQVDLRRSVVCKFRAARDLRADRVEPVTLWLDTDRTGSDRALT
                     TITWPLPEGEASVRLVPQRHRNLEPRFAPVERVRLHEVMDRLREWIGRTVAEREACAA
                     AMGRLEILAAELPGDEPTTLARSSLAIASCLLREHLGFRSPSVFVSALAARGLLSVTV
                     NDVLGALDEFVDVFNRSVEELVASDVDPQVHPLGDDYLPLHYACDRCGARRRLRRERT
                     AVDQFAVMTCSCGTAHRFHLGSSDLSLGELEGSERWSADVTLPVYLNDLASGVVAGRS
                     SVLYGLVLGEVLPKVLGRPPIPMLVPEDLAGASVPPGSPDSLLYEHLVGA"
     gene            complement(5905..7167)
                     /gene="bshA"
                     /locus_tag="VF058_01865"
     CDS             complement(5905..7167)
                     /gene="bshA"
                     /locus_tag="VF058_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HEX6207080.1"
                     /translation="MGSDRGRDPHARDEGRGVGERPLRIGIVCHSAVGGSVRVATGLA
                     SGLALRGHGIHLFARTPPPGSSRGAWGVTFHGLRGKRERLSPHLQADWSACDLESFLD
                     LVLSAITSVGLDVIHFHYAVPFGQIAANLRRRLGSACPRLVMTLHGTDVTAFGGDPDR
                     RRVLRRQLARLDALTTVSESHAELSADVFGFDRAPEVIPNFVDLQTFRPGRVRGIPGR
                     IDGRRRPRIAHVSNFRPVKDPEGVARIFTRVREQLDAELWLIGDGDGMSSVHSIVRSA
                     GVERDVRFFGLRREVHRILPESDVLLLTSKTESFCLAALEAAACGVPAVAPRVGGLPE
                     VVIHHETGELYEAGDEEEAARALLRLLGDDEPRRSMGRAAVRRARLFSRETIVGWYER
                     LYRDLLVGEGEVPRPSPLADRVVTVAGV"
     gene            complement(7113..>10067)
                     /locus_tag="VF058_01870"
     CDS             complement(7113..>10067)
                     /locus_tag="VF058_01870"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6207081.1"
                     /translation="AVLHLGAHPDDEDSGTIAYLSRGLGIRTVYWSATRGEGGQNRQG
                     PETGEALGIVRTWESLEARQVDGGEVLYGPFYDFGFSKSGEGALAKWGREDVLREVVR
                     AIRMVQPLVVVSRWSGGAGDGHGQHQAIGLVAEEAFRAAGAPARFPELEEQGLVPWQA
                     RKLYRSMQGDWQPGEDRVLGKAVPELEREGIVRVNAGDFDPVSGRTFQELAAVGRNRH
                     RTQGMAELPERGDYFYYYVLDRSLAPVRAPERGFFDGLDPTLTGLAEYPGCGPEVAEF
                     LAEARTGAEAAVRRFHPADPSEAGDAVLEGLESLRAAHDLVAADGSADPTRVALGALL
                     DRKIRTFEDAAARCFGIRLECLVDRRRAVPGDDPRITARVWCLGDRRASDLKVDLRLP
                     EGWTVRPGDDSAPVGEAETDPVLESTFHVRVPETAEFSCPYWLRQPRDPYRYRWPPGE
                     PGGLPFDGPVVSATARYTLGAHDLSVDAPAVSRETFVGGFRELPLTVLPSMALRPRER
                     RLILPIEDRDQEVDLRVSVRAMSEVGAEGDLNIEIPDGWTVRPHEVALRFPRGGESRT
                     VRLHLSIPRDQAPGVERLGYQIVSGRLRHGLVLQPVWQGAPGVPKPADETNCVAEAFV
                     MEPADVTVHRLSAAFVGSLRYGYVTGAEKDVLSSLVHFGLDMTSISDEELAYADLSSF
                     DAIVVGPNAYLVHDHVRRSAGRLLEYVEHGGTLVVQYQGYGYEAEGFAPYPFSYHQPH
                     DRVTFPDAPVTVLEERHPVLHLPNEIVDADFDGWLHDRGMYFWGEWDRRYVPVLECHD
                     PGEGPQRGGLMAASFGQGTYVYTGYSLFRQIPAGVPGAIRLFANLVGLAEARILERME
                     RARKVHLFSFMTDDQLHEVVRLMSETWFEDGAFIARQGQQGNELYLILEGEVEILKEA
                     GDSWERYVAGEGETVGELAVLTDLPRSASLRAKGDLRVLSVRGDHFRRLLREHWDLTE
                     GVIRMLATRVAE"
BASE COUNT         1575 a         3552 c         3496 g         1444 t
ORIGIN      
        1 aatcgctccg gcgcgtcatg acgcggtact tcgaggcgat gaaggtcatc ctggagcggc
       61 acggcgggct ggtcgagaag ttcatcggcg acgcgatcgt ggccgtcttc ggcgcccccg
      121 tcgcgcacga ggacgatgcc catcgggcgg tgcgcgctgc cgtcgtgatg cgcgacgccc
      181 tgcgaacgct gaacgaggag ttcaggcggg cgtggggtgt cacggtcgcc acgcgcaccg
      241 gcctgaacac cggggaggtg gtcaccggag accccaagac gggtggaacg ctcgtcaccg
      301 gcgacacggt caacgtcgcg gcccgcctgg aacaggccgc cgagcccgga gagatcctgc
      361 tgagcgaggc gacgtaccga ctcgtcagga acgtcgcgcc gaccgaagcg gtcggcccgt
      421 tcgcgatcaa ggggaaggcc accccggtcc gagcacgaag gttactcgac gtcggcgccg
      481 aggctccgcc tcgcgggttc ctcggtctgg actcgccgat cgtcggtcgc gatcgcgagc
      541 tcgagcagct tcgggcggcg ttccagcgga cgaacgaggc tcgtcggtgc gaggtcgtga
      601 cggtgctcgg ggcgccgggc gtcggcaagt cgcggctgac ggcggaattc ctcgccgagc
      661 tgccggtgga cgtcagggtc gtcagtggcc gttgcctccc ctacggcgaa ggcatcacct
      721 tctggccgat cgtcggcgtc cttcgggagt cggccggggt caccgagacg gactcgcccg
      781 agcaggccga cgcgaagttc gcgacgctcc tcgagggagt tgccgacgcc ggcctcatcc
      841 ggcagcgcct gaccgccctg ctcgggatct ccgacgtggc gcccgggatc caggagacgt
      901 tctgggccgt gcgcaagctg ttcgaggagc tggcgaggcg ccggcccctc gtcgtggtgt
      961 tcgacgacat ccactgggga gagcccacgt tcctcgacct cctcgagtac ctggtcgact
     1021 ggctccaggg cgtgcccgtg ttgctcgtat gcgtggcccg gccggagctg ctcgagggcc
     1081 ggggcgagtg gctcgccggc aaggacaacg tggtcctgat caagcttgga cccctggcgg
     1141 agccttacac cacgcggctc atccagaccc tgctcggcaa cgtgccgctt ccggaggagg
     1201 cgtcgaccag catcgccgac gtcacggagg gcaatcccct gttcgtcgag gagacgcttc
     1261 ggatgctgat cgacgagggc tccctccgcc ggcggaacgg ggtgtggagc gtcgacgagg
     1321 acctctccgc gctctcgatc ccgccgacga tccagtcgct cttgaccgcc cgtgtcgaca
     1381 tgcttgctgc gggcgagcgc gaagtgatcg agcgagcctc cgtggtgggg cgcgtgttct
     1441 ggtggggagc ggtctcaggg ctctcacctc gcgaggacca ggcccacgtg gccggatggc
     1501 tggcgtccct cgtccgcaag gagctgatcc gtcccgaacg gtccgacctc atggaggagg
     1561 acgcctacag attcacccac atcctcgttc gcgacgccgc gtacgagggg gtcccgaagg
     1621 cccgtcgcgc agagctgcac gagcggttcg cgcggtggct gcacgagaag agccgcgacc
     1681 gcgccggcga gtacgaggag atcgtcggct accacctcga acaggcgtat cgtctgttgc
     1741 gcgagctcgg tcccgccacc gaacgcacgt gggcgatggg cctggcggcg gcagcgccac
     1801 tggcttcggc cggcaaacgg gcgctcgcac ggggcgacat gccggccgcg gccaacctcc
     1861 tctcccgggc ggcagcgctc ctgcccgacg acgaccggaa ccggatggat ctgctgccgg
     1921 atctggcctt cgccctgctg cagacgggcg gcttcggccc aacgcgagag gtgctcgccg
     1981 agatccagcg ggccgccgcc atctcggagg acgtcctgct cggggcgcgt ggacaactcc
     2041 tggacctcgc cgtccggatg ttcaccaacc ccgagggatg ggccgaggag tcgtcgcgcg
     2101 aggcgacccg cgcaatctcg gtcttcggag agcacaagga cgagcgcggg gtggcgcagg
     2161 cctgggcact gcttgggctt tcgcacatga tcgccggcca cttcgcgacc gccgtcgagg
     2221 cgtgggagga agcagcccag cacgccacgg ccgccggcgc gcatcgggag gggctcgagc
     2281 acttggcctg gatccccgtc ggcgtctggt cgggtcccat gcccgtggag gaggcgatcg
     2341 gccgctgccg cgagatcggc gcgcgggcgc acggcgacag gaaggccacg gccgttgcgc
     2401 tcgccgtcca gggcaacctg gaggcgatgc gcgagcgcta cgcggaggct cgcgagctgg
     2461 gagtgcgggc acggtcggtt ctgcaggagg tcgcgctgcc cggatggttg ggggccctga
     2521 cgcagatgtc cgggtggacg gagatcctgg ctggcgaccc cgtggccgcc gaaggggaac
     2581 tgcgttcggg ggtcgagatg cttcgcggca tcggggagca gtcctggctc tcgacgaccg
     2641 ctgccatgct ggcggaggcg ctcttcgcgc aggaccgtct cgacgaggcc gaggagctcg
     2701 tgcggctgag cgaggagacc gccggaagcg aggacatctt ctcccaggtc ctgatgcgaa
     2761 cggttcgcgc caaggtgcgc gctcgccagg accggcccga cgaggcggca gggcgcgccg
     2821 aggaggcggt cgcgatcgcc cgctcgagcg actcgttgct ccttcagggc gaagccctca
     2881 cgagcctcgg ctacgtcatg cgcgtcgccg gtcgggaagc cgagggcgac gccgcgttgg
     2941 tcaacgccgt gcgcgtctgc gagcaaaagg gcaacctcgc gggcgcggcg cgggcccgcg
     3001 ccgttcgggc gtcctcgcag ccggcgcctt gagcggcggg cgtcaacctg atcgtccact
     3061 cggtcttttc gacaccgact tctcgatcgt cttgcggcgc gcaagccagc cagatcctcg
     3121 actagctcgc acccttcccc gcagctatcc tcacggctcc tgtgacgcga gtcgtggagg
     3181 agagcgatgc cgtgccgcct cacccactca cgtttcttcg acagccgccg agtcggagct
     3241 tcgcgtggcc ggcgcggccc gaggcggtca agcggcggga gatcctcggt gggctgatcc
     3301 acgagtatca tgcgcgcgtg gcatgagcga ggcctcgtga cctgggccga tgacccgccg
     3361 gtcgaacgat catgctcaat cgagcagccc tgatccttcg gtataaacag ccgttcgtcg
     3421 attggatcaa tgccgccgat ccctccccca cctcgcacac cctgaccgtg gacgaggtca
     3481 accaagagca caccgtgtac ctggtcgagg tcgaagacga ggacgaaccc gccgagtggc
     3541 tcgtccgtca tcacgaggag ctcttcgagg cagagctgaa cgggtggtac acggacccgg
     3601 cgctgtggcc cggcactgat cgctcaagat gctcaaggag tggtgttcgg tcgagctcca
     3661 caccgtcgtg gtggacaacg ggtgcgtctc cgctcgaaga cgatgagttg gaaagttgaa
     3721 tgagtcgtgc ccggcgatac gtgcccctgc gacacagtgt tcgaccccca cacccttcct
     3781 gggggaaagg accgatgttg ccggagcgct actcgtcgcg gctgtcgaca atcccgagcg
     3841 cttgtcgagt gaggctgcct tcgcaaggct gcggggttgc gccgcttccg gcctcctcgg
     3901 gcaagacaaa ccggcatcgg ttgaatcgag gtagggaccg gatcgccaac aacgcgccag
     3961 atcgtcgcta ccgcagctgc gacgcccgtc cctccgcgct cggttcggtt cagtggaaga
     4021 tctgatacct cgagggcgcc tcgccctcga ggcgagccag ccccggcagg ctcgtgacgg
     4081 gcacggggcc ggaagagtgg aacgcctccc ctcgctcaac gccgatcatc gccccgtgca
     4141 cggtcgccca cgcctcgacc acctggagaa accgtccggg gtcgaccggg ccgcgggatc
     4201 cgccctcggg tccgaactgg ctttcgtggg ctcggacggc ccgccacttc cggtcccaga
     4261 ccctcgacac gtcgaccacg aacgagggcg ggaaggggtg gtggaccatg tagtggtaca
     4321 cgtgcgccgg ccgatgcggc gtcccctcgc cgagccgcgg gagcccggcg aggaacgatg
     4381 tctcccggac cagccgcccg gcggcggcgt gatcgggatg ccggtcctgc tcgtacgggg
     4441 ccaggacgat ccgaggctcg agctcccgga tcagctcgag cagggccgtc cggtgcgcgg
     4501 gatcgacgcc cacgcggccg tcgggaagcc ccagcgacgg gtacgtggtc gctcccagga
     4561 tctccgccgc gcgttcgccc tcgtgctcgc ggtcgtccaa ggttccccgg ctcgccgtct
     4621 cgcccgaggt gagggtcgcc acgacgacgt cgaggtccgc gtccgcggcc aggatgatgg
     4681 tccccccaca tcccacctcg acgtcgtccg ggtgggcgcc gatcgcgagc acgtcggccg
     4741 tgttcatcac gctccgacga ggtgctcata caggaggctg tccggcgacc ccggaggtac
     4801 ggacgccccc gccaggtcct ccggcacgag catcgggatc gggggccgac cgaggacctt
     4861 cggcaggacc tcgccgagca ccaggccgta gaggacggag ctccgtccgg ccaccacccc
     4921 gctggccaga tcgttgaggt agaccggcaa cgtgacatcg gcgctccatc gctccgagcc
     4981 ctccagctct cccaacgaga gatccgagct cccgaggtgg aaccggtggg cggtcccgca
     5041 cgaacacgtc atgacggcga actgatccac tgcggtccgc tctcggcgga gtcgacggcg
     5101 cgccccacac cggtcgcacg cgtagtgcag cgggagatag tcgtcgccca gcggatgaac
     5161 ctgtgggtcg acgtcgctgg cgacgagttc ctcgacgctc cggttgaaca cgtcgacgaa
     5221 ctcgtcgagc gccccgagga catcgttcac ggtaacggac aggagccccc gagcggcgag
     5281 cgcggagacg aacaccgatg gggatcggaa ccccaggtgc tctcggagga ggcaggacgc
     5341 gatcgcgaga gacgaccggg ccagggtggt cggctcgtcc cccgggagct ccgcggccag
     5401 gatctccagc cgtcccatcg ccgccgcaca ggcttcccgc tcggcgaccg tccggccgat
     5461 ccattcgcgg agccggtcca tgacctcgtg caggcggaca cgctccacgg gagcgaaccg
     5521 gggttccagg ttccgatgac gctggggaac gagccggacc gaggcctctc cctccggcag
     5581 cggccacgtg atcgtcgtga gggctcgatc cgatccggtg cgatcggtat ccagccacaa
     5641 cgtgacgggc tccacccgat ccgcccgcag atcgcgagcc gcccggaact tgcagaccac
     5701 cgaccgccgt aagtccacct gggtctggtg cgcgacgtag acgggacgag ccccgtcggg
     5761 cagccccagt gcctcggcca gccctcggta cgccgggggc gccaactccc gcccgaatcc
     5821 cgcctcttgc aggatcaccc cgcgcgattc gaggaagaag cggccctccg ccgaagccag
     5881 caggtcgttc agggaacgca tcgctcacac cccggccacg gtgaccaccc ggtcggccag
     5941 aggggacggc cgcggcacct ctccctcccc cacgagcagg tcgcgataca gacgttcgta
     6001 ccatccgacg atcgtctccc gagagaacag gcgagcccgc cggacggcgg cccgacccat
     6061 ggatcgcctg ggctcgtcgt cgcccagcag ccgcaggagc gcccgggccg cttcttcttc
     6121 gtctccggcc tcgtacagct cgccggtctc gtggtggatg acgacctccg gcaggccgcc
     6181 cacgcggggg gccacggcgg ggactccaca ggcggccgcc tccagggccg ccaggcagaa
     6241 gctttcggtc ttgctcgtga ggagcagcac gtcgctctcc gggaggatcc ggtggacctc
     6301 ccgccggaga ccgaagaacc gaacgtcgcg ctccaccccg gcgcttctca cgatcgaatg
     6361 caccgagctc atgccgtcgc cgtctccgat caaccacaac tccgcgtcga gctgctcgcg
     6421 cacccgggtg aagatccgag cgaccccctc cgggtccttg accggtcgga agttcgagac
     6481 gtgggcgatc cgaggccgcc ggcggccgtc gatccgtccg gggatccctc gcacccggcc
     6541 aggccggaag gtctggaggt ccacgaagtt cgggatgacc tcgggcgcgc gatcgaaccc
     6601 gaacacgtcc gcggagagct cggcgtggct ctccgagacc gtcgtcaacg cgtcgaggcg
     6661 ggcgagttgg cggcgcagca cccgtctccg gtccgggtca ccaccgaagg cggtgacgtc
     6721 ggttccgtgc agggtcatca ccagacgagg gcacgccgag ccgagccggc ggcgcaggtt
     6781 ggcggcgatc tgcccgaacg gaacggcgta gtggaagtgg atcacgtcca gaccgaccga
     6841 cgtgatggcg gacaggacca ggtccaggaa cgactccaga tcgcaggcgg accaatcagc
     6901 ctggagatgg ggtgagagcc gctcgcgttt gccccgcagg ccgtggaagg tgacccccca
     6961 ggcccctcgg ctcgagccgg gcggcggcgt cctggcgaac agatggatgc cgtgtccgcg
     7021 gagcgccagc ccggaggcca gccccgtcgc cacgcgaacg ctgcccccga cggcggagtg
     7081 gcagacgatg ccgatccgga gggggcgctc gcctactccg cgaccctcgt cgcgagcatg
     7141 cggatcacgc cctcggtcag atcccaatgt tcccgcagca gccgccggaa gtggtcgcct
     7201 cggacggaga gcaccctcag gtcccccttc gcccgcaacg aggccgagcg gggcagatct
     7261 gtcagcacgg ccaactcgcc caccgtctcg ccctccccgg ctacgtaccg ttcccaagag
     7321 tcccccgcct ccttcagaat ctcgacctcg ccttcgagaa tgaggtacag ctcgttgcct
     7381 tgttggccct gtcgggcaat gaacgctccg tcttcgaacc acgtctccga catcagccgg
     7441 acgacctcgt gaagctggtc gtcggtcatg aaggagaaca ggtgcacctt ccgcgctcgt
     7501 tccatccgtt ccaggatgcg cgcctccgcc agtcccacga ggttcgcgaa caggcggatc
     7561 gccccgggaa cgccggccgg gatctggcgg aagagcgagt acccggtgta cacgtacgtc
     7621 ccctgcccga agctggcggc catcagaccg cctcgttggg ggccttcgcc cggatcatgg
     7681 cactcgagca cggggacgta tcgacggtcc cactcccccc agaagtacat cccgcggtcg
     7741 tgcagccatc catcgaagtc ggcgtccacg atctcgttgg gcaggtgcag caccggatgc
     7801 cgctcctcga gcacggtcac cggggcgtcg gggaaggtca cgcggtcgtg aggctggtgg
     7861 taggagaacg ggtaaggagc gaaaccctcg gcctcgtacc catacccctg gtactgaacg
     7921 acgagggtcc ccccgtgctc gacgtattcg agcagccggc cggcgctccg gcgaacgtgg
     7981 tcgtggacca ggtaggcgtt aggccccacg acgatggcgt cgaaggagct cagatcggcg
     8041 taggccaact cctcgtcgga gatggaggtc atgtcgaggc cgaagtggac cagcgaagag
     8101 agcacgtcct tctccgcacc ggtcacgtac ccataccgca gcgagcccac gaaggccgcg
     8161 ctgagacgat ggaccgtgac gtcggccggt tccatgacga acgcctcggc tacgcagttg
     8221 gtctcgtccg cgggcttcgg cacccccgga gccccctgcc agacgggctg gagcaccagg
     8281 ccgtggcgga gccggcccga cacgatttgg tacccgagcc tctcgacgcc gggagcctgg
     8341 tcgcgcggga tcgacaggtg gagccgaacg gtgcgggatt cgcccccccg ggggaaccgg
     8401 agggccacct cgtgggggcg gaccgtccag ccatcgggga tctcgatgtt gaggtcgccc
     8461 tcggcgccca cctcactcat cgcccgaacg gagacccgca gatccacctc ctgatcgcga
     8521 tcctcgatcg gaaggatcag ccgacgttcc cgcggccgga gggccatcga gggaagcacc
     8581 gtcagcggca gctcccggaa tccccccacg aaggtctcgc ggctcaccgc cggcgcgtcc
     8641 acggagaggt cgtgggcgcc gagggtgtac cgggcggtcg cggacacgac gggaccgtcg
     8701 aacggaaggc cgccgggttc ccctggtggc cagcgatacc ggtagggatc gcgcggttgg
     8761 cgcagccaat acgggcacga gaactccgcg gtctcgggaa cccggacatg gaaggtggac
     8821 tcgaggacgg gatcggtctc cgcctcgccg acgggggcgg aatcgtctcc gggacggacc
     8881 gtccaaccct ccggcagcct gagatcgacc ttcaggtcgg aagcgcgacg gtccccgagg
     8941 caccagaccc gggcggtgat gcgagggtcg tccccgggca cggcccgtcg gcggtcgacg
     9001 aggcactcca ggcggatgcc gaaacaccgc gcggcggcgt cctcgaacgt tcggatcttg
     9061 cgatcgagca gggcgccgag cgccactcgc gtcgggtcgg ccgacccgtc ggccgccacc
     9121 aggtcgtggg ccgcgcgcag cgactccagt ccctcgagca cggcgtctcc cgcctcgctc
     9181 gggtcggccg gatggaaacg ccgtacggcc gcctccgccc cggtccgggc ttcggcgagg
     9241 aactcggcga cctccgggcc gcagcccggg tactccgcca ggccggtcag ggtcgggtcg
     9301 aggccgtcga agaatcctcg ctccggcgcg cggacggggg cgaggctccg atcgagcacg
     9361 tagtagtaga agtagtcgcc ccgttccggg agctcggcca tcccctgcgt gcggtggcgg
     9421 ttccggccga ccgccgccag ctcctggaag gtccggccgg acaccgggtc gaagtccccc
     9481 gcgttgacgc ggacgatgcc ctcccgttcg agctccggca cggccttgcc aagcacgcgg
     9541 tcctcgccgg gctgccaatc cccctgcatc gagcgataga gcttgcgcgc ctgccacgga
     9601 acgagcccct gctcctccag ctccgggaac ctggccgggg ccccggcggc cctgaacgcc
     9661 tcctcggcga cgaggccgat cgcctgatgt tgtccgtgcc cgtccccggc gccaccggac
     9721 caccggctga cgacgacgag gggctgaacc atccggatgg cccgaaccac ctcgcggagg
     9781 acgtcttccc ggccccactt ggcgagggca ccttcgccgc ttttgctgaa cccgaagtcg
     9841 tagaaggggc cgtacagcac ctcgccgccg tccacctgac gggcctcgag gctctcccag
     9901 gttcgaacga tcccgagcgc ctcccccgtt tccgggccct gccggttctg gccgccctcc
     9961 cccctcgtcg cggaccagta cacggtgcgg atccccagcc cgcgcgacag gtaggcgatc
    10021 gtgcccgagt cctcgtcgtc gggatgggct cccaggtgca gcactgc
//
LOCUS       DASUTW010000054         2181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_792809, whole genome shotgun sequence.
ACCESSION   DASUTW010000054 DASUTW010000000
VERSION     DASUTW010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792809"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VF058_01875"
     CDS             complement(<1..221)
                     /locus_tag="VF058_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207082.1"
                     /translation="MSESDRHELYGSLEDAIGKRSTETIMALLPPVGWADVATKHDLA
                     ELESRLVARFEGRFERLDARFDRMEGRFDA"
     gene            complement(328..603)
                     /locus_tag="VF058_01880"
     CDS             complement(328..603)
                     /locus_tag="VF058_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207083.1"
                     /translation="MAVHEREERLRKRLGEVLGTEEAHTLMERLGTMSHEHELLKRDM
                     QIMFAELRVEMAERFDAQTKTLFRTFIVSGAVMILSVAGLAFGVALT"
     gene            1006..1407
                     /locus_tag="VF058_01885"
     CDS             1006..1407
                     /locus_tag="VF058_01885"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207084.1"
                     /translation="MKTFIVHGTNRAGELAKITGALSSKGVNVLISAFGENGRALAGF
                     VASDETSAQEALKDSGFEYKMWPAVTVRLSDTPGQVAEISRKLGDAGINIECFLPVSM
                     TEDKVIAAIGVEDIEGARKALVDHVVEYAYS"
     gene            1490..>2181
                     /locus_tag="VF058_01890"
     CDS             1490..>2181
                     /locus_tag="VF058_01890"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6207085.1"
                     /translation="MTEPLRLYGELAGWWQLLSPPEEYAEEAGIYRRLLGDARTVLEL
                     GSGGGNNASHLKRHFEMTLVDASEEMLEVSRGLNPECRHIQGDMRTVRLDETFDGVFV
                     HDAIDYMTSLEDLRAAMETAYVHLRPGGVAVFCPDHVRESFRPGTDHGGTDGAGRGMR
                     YLEWTLDPDPDDSTYIADYVFVLRDEGEVRVVYDQHVCGLFSIQEWKDALTEVGFESV
                     EIAAGDEDREVFV"
BASE COUNT          363 a          712 c          761 g          345 t
ORIGIN      
        1 gcgtcgaacc gcccctccat ccggtcgaac ctggcgtcga gcctctcgaa ccgaccctcg
       61 aaccgggcga cgagacgact ctcaagctcg gcgaggtcgt gcttggtggc cacgtcggcc
      121 caccccaccg gcggcagcag cgccatgatc gtctccgtcg acctcttccc gatcgcgtcc
      181 tcgagcgagc cgtagagctc gtgtcgatcg gactcgctca ccgccattgt gtccctccct
      241 tccacgggca acaagagggg gatgcggcga gcctactcgg aggcgccgac atggaaccgc
      301 gcgaccgacc ggatcgcgtc gggctgctca ggtgagggcg accccgaacg cgaggccggc
      361 caccgagagg atcatgaccg cgcccgagac gatgaacgtc cggaacaggg tcttggtctg
      421 ggcgtcgaag cgctccgcca tctccacccg gagctccgcg aacatgatct gcatgtccct
      481 cttcagcagc tcgtgctcgt ggctcatcgt gccgagtcgc tccatcagcg tgtgcgcctc
      541 ctcggtgccc agaacctcgc cgagtcgttt gcgcagccgt tcctcgcgtt catgcacggc
      601 cattgtgtcc ctcctgccac gagccaacaa gagggggagg gggcgagcgt actccgaggc
      661 gccgacatag gatcacgagg tacctgggag gggtcatggg ggagcgatcg agatctcgcg
      721 ccgcggagag gcttcgggcc cttctgcgcc ccttccgacg ccgaaccggg cgcctcgacg
      781 tcaccgagga ggggcatcgc ctgcggcgcg agatgtcctc caggcgccgc cgctacgacc
      841 ccgatccccc ctggatgtcc tgatccgatt tttcttcgtc tggcttggga gggattcatg
      901 cgggccgcct ggacaggggg gagtactgtt gtaagtagag cttggccgga acacgttggc
      961 ccatcggccc gccctcgcgc cgagctcgtc aaggagagat gctgcatgaa gaccttcatc
     1021 gtgcacggca caaaccgtgc cggggagttg gcgaagatca ccggtgcgct ctcgtcgaag
     1081 ggcgtcaacg tgctgatcag cgccttcgga gagaacggtc gggccctcgc aggcttcgtc
     1141 gcgagcgacg agacgtcggc ccaggaggcc ctgaaggact cgggcttcga gtacaagatg
     1201 tggccggcgg tcaccgtccg tctgagcgac acgcccggtc aggttgccga gatcagccgc
     1261 aagctcggcg acgcgggcat caacatcgag tgcttcctgc cggtcagcat gaccgaagac
     1321 aaggtcatcg cagcgatcgg agtggaggac atcgagggcg ctcgcaaggc gctcgtcgac
     1381 cacgtggtgg agtacgccta cagctagtcg cacgggatcc atcgagaggg ccggcatcac
     1441 gccggccctt tcgcattgcg tggacccgct tgacggtacg ctgggcgtca tgacggagcc
     1501 gctgaggctc tacggcgagc tcgcgggctg gtggcagctg ctgtcgcccc ccgaggagta
     1561 cgcggaggag gccgggatct accggcgcct gctcggggac gcccgcaccg tgctcgagct
     1621 ggggagcggg ggagggaaca acgcctcgca cctgaagcgg cacttcgaga tgacgctcgt
     1681 ggatgcgtcg gaggagatgc tcgaggtgag ccgcggcctc aaccccgagt gccggcacat
     1741 ccagggcgac atgcggacgg ttcgcctcga cgagaccttc gacggggtgt tcgtccacga
     1801 cgcgatcgac tacatgacga gcctcgagga cctgcgcgcg gcgatggaga cggcctacgt
     1861 gcacctgcgg cccggcggcg tggcggtgtt ctgccccgac cacgtgcgcg agagcttccg
     1921 gccggggacc gaccacggcg gcaccgacgg tgccgggcgg gggatgcggt acctggagtg
     1981 gacgctggat cccgatcccg acgactccac ctacatcgcc gactacgtct tcgtgctccg
     2041 cgacgaaggg gaggtgcggg tcgtctacga ccagcacgtg tgcgggctgt tctcgatcca
     2101 ggagtggaag gacgcgctga ccgaggtcgg gttcgagagc gtggagatcg cggcgggcga
     2161 cgaagaccgg gaggtcttcg t
//
LOCUS       DASUTW010000055         2935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_146132, whole genome shotgun sequence.
ACCESSION   DASUTW010000055 DASUTW010000000
VERSION     DASUTW010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146132"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VF058_01895"
     CDS             <1..709
                     /locus_tag="VF058_01895"
                     /inference="COORDINATES: protein motif:HMM:NF014709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HEX6207086.1"
                     /translation="HVLDKGSPVPDVEALLSVAAGSIDLWKLGWGTAYLDPGLEAKLP
                     LLARHDVRACVGGTLLEAAWLQGRATECLDWAAETKFPCVEVSNGAVGMPIEEKHRLI
                     AEASDRFVVVSEVGSKDPEATVSPAAWAEEVAADLEAGAEWVLTEGRESGTVGLYTPR
                     GEVREDVVEAVTRVAGPARLIFEAPRKSQQAWLIRRFGPDVNLGNVRLDEVLGVEALR
                     LGLRADTIKAPSDEAGS"
     gene            706..1581
                     /locus_tag="VF058_01900"
     CDS             706..1581
                     /locus_tag="VF058_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207087.1"
                     /translation="MNLVPRRYRGVSLAEVGSLDPVALRDHFVGREAYRRTEFIVARA
                     GDDLSLLRVEKSSVDPLFSPITSVEVLAGPDECAAVEDPEVDTAIPTQLGRAALDRAP
                     GARCVVVTGRYEHVSFILDPAPISVRVVEVAPPHPAKLVDQVRRVLDTAEDLPPIDLR
                     PDEIDIVELARTRPSERYLYPCRGSGVAPEGAEVVYLDQHPPKEDWVLVGCERSAQIH
                     RWFYDEDAPYVEMCPRELTRPSEVPTLTKCCMLEFGVERDGSMVVVPWGASLEEIRKG
                     LRIVMEVAEPAWEPV"
     gene            1566..2912
                     /locus_tag="VF058_01905"
     CDS             1566..2912
                     /locus_tag="VF058_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011751546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase family protein"
                     /protein_id="HEX6207088.1"
                     /translation="MGARLTDSELYGHLWGTPELREVFEERARLASWIDILATLAEVQ
                     AELGIVPEEAAAAIRRHASVDRLDLAAVAEETRRTGHSTLGLIRGLREGLPPEGAEWV
                     YYGITVQDLTDTWASLALRRTGEVVWRDLFAIEGSLVELAERGRDLPMAGRTHGQAGA
                     PITLGFKAAGWADEVRRGLDRLREGASRWLVGQLGGAVGTQGFFGEAAPQLRRRFCER
                     LGLADPGISWTASRDRIAEAGATLASIAATLARIGNEVYQLQRGEIGELREPAGKAVV
                     GSITMPHKRNPEASEHLVTLARLVRGAAGTLLEGQVQEHERDGRGWKAEWAALPEVCL
                     LTGTAAALARDMVERLEVDEEAMARNLGPHASSEAVLAALAPRLGKHRAQELLQEALA
                     EGATLEEALRGHPELRDALDDEALAAARGAATGSAGAQVGEVLARIRRARDGERWP"
BASE COUNT          377 a          984 c         1185 g          389 t
ORIGIN      
        1 ccacgtcctg gacaagggga gccccgtgcc cgacgtcgag gccctgctct ccgtcgccgc
       61 ggggtcgatc gacctctgga agctggggtg ggggacggcc tacctggatc ccgggctcga
      121 ggcgaagctc ccgctgctcg ctcggcacga cgtccgggcg tgcgtggggg ggaccctgct
      181 ggaggcggcg tggctccagg gcagggcgac ggagtgcctg gactgggccg cggagacgaa
      241 gttcccctgc gtcgaggtgt cgaacggcgc cgtggggatg ccgatcgagg agaagcaccg
      301 tctgatcgcc gaagcgtcgg atcggttcgt cgtcgtctcc gaggtggggt cgaaggaccc
      361 cgaggcgacg gtctcgccag ctgcctgggc cgaggaggtc gcggcggacc tcgaggccgg
      421 ggccgagtgg gtcctgaccg aggggcgtga gagcggcacc gtcggcctct acacgccgcg
      481 gggcgaggtg cgcgaggacg tggtcgaggc cgtgacgcgg gtggcggggc cggcgcggct
      541 gatcttcgag gctccccgga agtcccagca ggcctggctg atccggcgct tcggccccga
      601 cgtcaacctt ggcaacgtcc gtctggacga ggtgctcggc gtggaggcgc tccggctggg
      661 cctccgcgcc gacacgatca aggcgccgtc cgacgaggcg ggctcgtgaa cctcgtccct
      721 cgccgatacc ggggtgtctc cctggccgag gtggggtccc tggacccggt ggccctccgg
      781 gaccacttcg tcggtcgcga ggcctaccgg cgcaccgagt tcatcgtggc ccgggccggg
      841 gacgacctct cgctgctgcg cgtggagaag tcgtcggtcg atccgctgtt ctctccgatc
      901 acctcggtgg aggtgctggc cggcccggac gagtgcgccg cggtggagga ccccgaggtc
      961 gacaccgcca tcccgaccca gctcggacgg gcggccctgg accgtgcccc gggtgcgaga
     1021 tgcgtcgtgg tgaccgggcg gtacgagcac gtcagcttca tcctggaccc ggcgccgatc
     1081 tcggtccgcg tggtcgaggt ggccccgccc cacccggcca agctcgtgga ccaggtgcgc
     1141 cgggtgctgg acaccgcaga ggacctcccc ccgatcgacc tgcgccccga cgagatcgac
     1201 atcgtggagc tggcgaggac gcgcccgtcg gagcgctacc tctacccgtg ccgcggatcg
     1261 ggcgttgcgc ccgagggggc cgaggtggtc tacctcgatc agcatccccc taaggaggac
     1321 tgggtgctcg tggggtgcga gcggtccgcc cagatccacc ggtggttcta cgacgaggac
     1381 gctccctacg tcgagatgtg tccgcgcgag ctgacccggc cctcggaggt ccccaccctg
     1441 acgaagtgct gcatgctcga gttcggggtg gagcgcgacg gctccatggt cgtcgtcccg
     1501 tggggcgcct ccctcgagga gatccgaaag gggctccgga tcgtgatgga ggtggccgag
     1561 ccggcgtggg agcccgtctg accgactccg agctgtacgg gcacctctgg gggacgcccg
     1621 agctgcgcga ggtcttcgag gagcgagccc gcctggcctc gtggatcgac atcctggcca
     1681 cgctggccga ggtccaggcc gagctgggca tcgtcccgga ggaggccgcg gccgcgatcc
     1741 ggcggcacgc gagcgtggac cgcctcgatc tggccgccgt cgccgaggag acccgccgca
     1801 ccggccactc gacactgggc ctgatccggg gcctccggga gggcctgccg cccgagggcg
     1861 ccgagtgggt ctactacggc atcaccgtgc aggacctcac cgatacgtgg gcgtcgctcg
     1921 ccctgcggcg gacgggcgag gtcgtgtggc gggacctgtt cgcgatcgag gggtcgctgg
     1981 tggagctcgc cgagcggggg cgggacctcc cgatggcggg gcggacccac gggcaggccg
     2041 gcgcccccat caccctgggg ttcaaggccg ccggctgggc cgacgaggtc cgccggggac
     2101 tggaccggct gcgggagggg gcgtcgaggt ggctggtggg ccagctgggc ggcgccgtgg
     2161 ggacccaggg gttcttcggc gaggccgccc cccagctccg gcgccgcttc tgcgagcggc
     2221 tggggctggc cgacccgggg atctcctgga cggcctcccg ggaccggatc gcggaggcgg
     2281 gagcgacgct ggcctcgatc gccgccacgc tggcccggat cggcaacgag gtctaccagc
     2341 tccagcgggg cgagatcggg gaacttaggg agccggcggg gaaggccgtc gtgggaagca
     2401 tcacgatgcc tcacaagcgg aaccccgagg cctccgagca cctcgtgacg cttgcgaggc
     2461 tggtccgggg cgcggccggg actctgctcg aggggcaggt gcaggagcac gagcgggacg
     2521 gcaggggatg gaaggccgag tgggcggcgc tcccggaggt gtgcttgctg acggggacgg
     2581 cggcggccct cgcgcgagac atggtcgagc ggctggaggt cgacgaggag gcgatggccc
     2641 ggaacctcgg gccgcacgcg tcctccgagg cggtgctggc ggcgctggcc cccaggctcg
     2701 ggaagcaccg cgcccaggag ctgctgcagg aggcgctggc cgagggcgcg acgctggagg
     2761 aggcgctccg gggccatccc gagctgcggg acgccctcga cgacgaggcg ctggccgccg
     2821 ctcgaggggc cgcgacgggg tcggccggcg cgcaggtcgg cgaggtgctg gcgaggatcc
     2881 ggcgcgcgcg agacggggaa cggtggccgt gagccggccc ccgcggatgg cactc
//
LOCUS       DASUTW010000056         4921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1571634, whole genome shotgun sequence.
ACCESSION   DASUTW010000056 DASUTW010000000
VERSION     DASUTW010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571634"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VF058_01910"
     CDS             <1..499
                     /locus_tag="VF058_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207089.1"
                     /translation="QDEARYADRVSLLLEGFRGHLYGRIIPELSREAEALFRELTNHE
                     YEDLRIGEEDLSIQIADGGAYFPIERFSGSETDLANLALRVAISTHLSRMSGADIGMM
                     VLDEVLASLDHERRDLMVQAMGRLSNRFHQLFVITHAEQVKDQFPASLEVRKVGRRRS
                     VAELR"
     gene            complement(549..1001)
                     /locus_tag="VF058_01915"
     CDS             complement(549..1001)
                     /locus_tag="VF058_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207090.1"
                     /translation="MAIIHFELEDLKEVGRVGDLGSLLECIQQVLPEDAVLYLEGSRR
                     HMAPELREFLEANPVPRPAEIPKGTSWPKSAQYHLETSDPNLNQLRVIESRHLQPEVC
                     DHLIVYRGHEVLMTAYDAGHGDVYVSKSLPAEDIDRMKRILLAADQEP"
     gene            complement(1009..1938)
                     /locus_tag="VF058_01920"
     CDS             complement(1009..1938)
                     /locus_tag="VF058_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018352449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HEX6207091.1"
                     /translation="MSPERPGAPSRKKGATRTHVLLLDTSSVMYRAYYALPDSITAPD
                     GRPVNAVHGYLDMTANLIRNREPGEVIHAFDDDWRPAPRVAEYAGYKAERPEEPADLT
                     PQFAMLAEVLSAFGMGIAEAPGWEAEDAIGSLVARMSGRDRAEIVTGDRDLIQLVRDP
                     QVKLLYTRKGVSQLDVLDEQAVEEKYGVPASRYADFAILRGDPSDGLPGVRGVGEKTA
                     RALVAEYSSIDEMVADAQAERRTGAALKKSPALRARIADAAEYLEAMKKVVPIRTDLE
                     VRLHRVKRDDAALDRLAESFGLAGPIGRLRAVI"
     gene            complement(2011..2502)
                     /locus_tag="VF058_01925"
     CDS             complement(2011..2502)
                     /locus_tag="VF058_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207092.1"
                     /translation="MRNLAGEPVSVGGLAVDAEEGMSEHVSMIPPGQVDVACWPYSQH
                     GGRKPENVPMTVTDPDGYWVSPGQLECVGRTSTEEVRDYFVEAEGEVGDPVAIARRRL
                     NGLQAGDVLRRTGYPEAIHAGVEVVRDGRTVALLGFIPAQGGGWLLGTQGACTGSGIR
                     WKP"
     gene            complement(2801..3193)
                     /locus_tag="VF058_01930"
     CDS             complement(2801..3193)
                     /locus_tag="VF058_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEX6207093.1"
                     /translation="MVELPEVTLRVRMSQSDAHYGGNLVAGAKMMELFGDLATELTIR
                     SDGDEGLLAGYESVEFLAPVHAGDFIEATGHIVATGTTSRRCEFEARKVAVPRPDVND
                     SAADPLDEPIIVARAIGTTVVKKDRQRR"
     gene            3251..4855
                     /locus_tag="VF058_01935"
     CDS             3251..4855
                     /locus_tag="VF058_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6207094.1"
                     /translation="MPSVRRADVVVVGGGHNGLVCAAYLAKAGKSVVVLERRGVLGGA
                     AVTEEPWPGFKVSTLSYVVSLMPERIIKDLELRKHGFEVYPIDPAYFSPFPDGRGVLI
                     WEDPEKAAKEIAKLNERDAEGYLRYEHDLEELASIVRPLLLRVPPSIELGSWADVREA
                     IGLGQYFFRRRRNLAKLADLMTLSVADFLDQYFADEAVKGALSPGGVIGAWGGPMTPG
                     SAYVLLHHRMGDAAGPHGAWAFVRGGMGALSGAIARAAAAAGADIRTNAPVKRIAVAG
                     ERARGVELEDGTTIAARAVVSAAHPQLTFFDLVGSEHLPSEMVQDLQRYRTRGASAKV
                     NLALSELPDFTAMPGTELGPQHPEFIICPSIPYLERAWDDAKYGRWSSEPMLDCVIPS
                     TKDPTLAPEGKHVLTAFVQYAPYQLEEGKAWDGEREAFGDRVVETIAAYAPNVPDAIL
                     HRQVITPLDLEREYGLVGGNIFHGEMSLDQLFSQRPTPMMVRHRTPIRGLYMAGSGTH
                     PGGGVMGAPGFNAARVIRRDLRLRRR"
BASE COUNT          737 a         1706 c         1689 g          789 t
ORIGIN      
        1 ccaggacgag gcccgctacg ccgaccgcgt ctcgctcctc ctcgagggct tccgcgggca
       61 cctgtacggg cgcatcatcc ccgagctctc gcgcgaggcc gaggccctct tccgcgagct
      121 cacgaaccac gagtacgagg atctccggat cggcgaggag gacctctcga tccagatcgc
      181 cgacgggggc gcctacttcc cgatcgagcg cttctcgggc tcggagaccg acctggcgaa
      241 cctcgccctg cgcgtggcga tctcgacgca cctctcgcgg atgagcgggg ccgacatcgg
      301 gatgatggtg ctcgacgagg tgctcgcctc gctcgaccac gagcgccgcg acctgatggt
      361 ccaggccatg ggccgcctct ccaaccgctt ccaccagctc ttcgtgatca cgcacgcgga
      421 gcaggtgaag gaccagttcc ccgcctcgct cgaggtccgc aaggtcggtc gccgccgctc
      481 cgtcgccgag ctcaggtagt ttctgagatc aatcgacgga gactccccaa tgcctgcttg
      541 cggtagcttc agggctcctg gtcggctgcc agaaggattc gcttcattcg atcgatgtct
      601 tcggccggga ggctcttcga cacgtatacg tctccatggc cagcgtcgta agcggtcatg
      661 agcacctcat gacctcggta gacaatcaag tggtcgcaga cctcgggctg tagatggcga
      721 gattcgataa cccggagctg gttcaggttg ggatcactcg tctccaggtg atactgcgcc
      781 gacttcggcc aggaggtccc cttcggaatc tcggcggggc gagggactgg gttggcttcc
      841 agaaactctc gaagctccgg tgccatgtgg cgtcgtgatc cctcgagata gaggaccgca
      901 tcctcaggga gaacctgctg aatgcactcg agcaagctcc cgaggtcgcc gacgcgcccc
      961 acttccttga ggtcttcgag ttcgaaatga atgatcgcca tgaccgcgtc agattactgc
     1021 tcggaggcgg ccgatggggc cggcgaggcc gaagctctcc gcgaggcggt cgagggccgc
     1081 gtcgtcgcgc ttcacccggt gtagccgtac ctcgaggtcg gtgcggatcg ggaccacctt
     1141 cttcatcgcc tcgaggtact cggccgcgtc ggcgatcctc gcccgcagcg ccggcgactt
     1201 cttgagcgcc gctcccgtcc gccgctcggc ctgggcgtcg gccaccatct cgtcgatcga
     1261 gctgtactcg gccacgagcg cgcgcgccgt cttctccccc accccgcgca cgccgggcag
     1321 cccgtccgac ggatccccgc gcaggatcgc gaagtccgcg tagcgcgagg ccggcacgcc
     1381 gtacttctcc tcgaccgcct gctcgtcgag gacgtcgagc tgcgagaccc ccttccgtgt
     1441 gtagagcagc ttcacctgcg ggtcgcgcac gagctggatc aggtcgcggt cgccggtgac
     1501 gatctcggct cgatcccgcc cggacatccg cgccacgagc gagccgatcg cgtcctcggc
     1561 ctcccagccc ggcgcctcgg cgatccccat cccgaatgcc gagagcacct cggccagcat
     1621 cgcgaactgg ggggtgaggt cggcaggctc ctcgggccgc tcggccttgt agcccgcgta
     1681 ctcggccacg cgcggcgcgg gccgccagtc gtcgtcgaag gcgtggatca cctcgcccgg
     1741 ctcgcggttg cgaatcaggt tggccgtcat gtcgaggtag ccgtggaccg cgttcacggg
     1801 tcggccgtcg ggcgcggtga tcgaatcggg cagcgcgtaa taggcccggt acatcacgct
     1861 ggaggtgtcg agaagcagca cgtgggttcg cgtcgccccc ttcttgcgcg agggcgcccc
     1921 cggtctctcc gggctcatgg aagaagggta ccggccggag tcctgcctct acagcctcgc
     1981 tgtgggagct cacatcgggt cctttgggag tcacggcttc cagcggatgc cgctcccagt
     2041 gcatgcgcct tgggtgccga gtagccatcc tccgccttgc gcggggatga atccgagcaa
     2101 ggccaccgtc cgtccatcgc ggaccacctc gacacccgca tggatggctt ccgggtagcc
     2161 ggtccgtcgg aggacgtcgc cggcctgcaa cccgttgagc cttcgacgag cgattgcgac
     2221 tggatcgcca acctcccctt ctgcctccac gaagtaatct cggacctcct cagtcgaggt
     2281 tcggccgacg cactcgagct gtccagggct gacccagtag ccgtcaggat cggtcaccgt
     2341 catgggcacg ttctccggct tccgtccccc gtgctggctg tagggccagc aggcgacgtc
     2401 gacctggccc ggagggatca tcgacacgtg ctccgacatc ccctcctccg catccacagc
     2461 gagaccgccc acgctcacgg gctcacccgc gaggttccgc acactggcgt ggagaccgtc
     2521 cgggcggacc gtcacccgcg gtgtgaggag ctcggtcgaa ccgtccactc gacacacgat
     2581 gcgagcaatc gtctcctcgg caggatctcc tcccggggac gccgggagtt ctgaagggcc
     2641 gcaagcggcg gccgcgatca ggatcgcggt gccagtggcg cggatcaggt acctcatccg
     2701 agacctcctt gaggcgggta caggatacga cgcgcccgaa gcagaggaag ttcccgaagc
     2761 gactttcgaa ccttcctccg cgcttcccgg gttcgccgga tcaccggcgc tggcgatcct
     2821 tcttaacgac ggtggtgccg atcgcgcggg ccacgatgat gggctcgtcg agtggatcgg
     2881 cggccgagtc gttcacgtcg ggccggggca cggcgacctt cctcgcctcg aactcgcagc
     2941 gcctcgaggt ggtccccgtg gccacgatgt gtccggtggc ctcgatgaag tcgccggcgt
     3001 ggacgggcgc caagaactcc accgactcgt agccggcaag cagcccctcg tcgccgtcgc
     3061 ttcggatcgt gagctcggtg gcgagatcgc cgaagagctc catcatcttc gcgccagcca
     3121 cgaggttgcc gccgtagtgc gcgtcgctct gggacatgcg gacccgcagg gtcacctcag
     3181 ggagctcgac catggcggga gtgtaatcga gcggaacgat cgcaggcgcg cgaggtcgca
     3241 ggcggctagg atgccctccg tgcgacgggc ggacgtggtg gtggtcgggg gtgggcacaa
     3301 cgggctcgtg tgcgccgcgt acctggcgaa ggcggggaag tcggtcgtgg tgctggagcg
     3361 gcgcggggtg ctgggcggcg ccgcggtcac cgaggagccg tggcccgggt tcaaggtctc
     3421 gacgctctcc tacgtcgtca gcctgatgcc cgagcgaatc atcaaggacc tcgagctacg
     3481 gaagcacggc ttcgaggtct acccgatcga ccccgcgtac ttctctccct tccccgacgg
     3541 ccgcggcgtc ctgatctggg aggacccgga gaaggccgcg aaggagatcg cgaagctgaa
     3601 cgagcgggac gccgagggct acctgagata cgagcacgat ctcgaggagc tggcctcgat
     3661 cgtgcgaccc ctcctgctgc gggtgccgcc ctcgatcgag ctggggtcgt gggcggacgt
     3721 gcgggaggcg atcgggctgg ggcagtactt cttccggcgg aggcggaacc tggcgaagct
     3781 cgccgacctc atgacgctct cggtggcaga cttcctcgac cagtacttcg cggacgaggc
     3841 ggtgaagggg gcgctgtcgc cgggcggggt gatcggggcg tggggcggcc cgatgacgcc
     3901 gggctccgcc tacgtgctgc tgcaccatcg gatgggcgac gcggcggggc cgcacggggc
     3961 gtgggcgttc gtcaggggcg gcatgggggc gctgtccggc gcgatcgcca gggcggcggc
     4021 cgcggccggc gccgacatcc ggacgaacgc gcccgtgaag cggatcgccg tggccggcga
     4081 gcgggcccgc ggcgtggagc tcgaggacgg gacgacgatc gcggcccgtg ccgtggtctc
     4141 ggccgcacat ccccagctca ccttcttcga cctcgtgggg agcgagcacc tcccctcgga
     4201 gatggtccag gacctccagc gctaccggac ccgcggcgcc tcggccaagg tgaacctcgc
     4261 gctgtcagag cttcccgact tcacggcgat gcccgggacc gagctcggcc cccagcaccc
     4321 cgagttcatc atctgcccct ccatccccta cctcgagcgg gcctgggacg acgcgaaata
     4381 cggccggtgg agctcggagc cgatgctcga ctgcgtgatc ccgtcgacga aggaccccac
     4441 gctggccccg gagggcaagc acgtgctgac ggcgttcgtc cagtacgcgc catatcagct
     4501 cgaggagggg aaggcgtggg acggcgagcg cgaggcgttc ggcgaccgcg tcgtggagac
     4561 gatcgccgcg tacgcgccca acgtgcccga cgcgatcctc caccgccagg tgatcacacc
     4621 cctcgacctg gagcgcgagt acgggctcgt gggcggcaac atcttccacg gcgagatgtc
     4681 gctggatcag ctcttctccc agcggcccac gccgatgatg gtgcggcacc gcacgccgat
     4741 ccgcgggttg tacatggcgg gatccggcac ccacccgggc ggcggcgtga tgggcgcgcc
     4801 gggcttcaac gcggcgaggg tgatccgccg cgacctccgc ctccgccgcc gctgaccgaa
     4861 cgcccggacg cggagctcag accgccgtcg ccgggtcgag cctgacctcg ggtcggcgcc
     4921 g
//
LOCUS       DASUTW010000057         2276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1572048, whole genome shotgun sequence.
ACCESSION   DASUTW010000057 DASUTW010000000
VERSION     DASUTW010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572048"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VF058_01940"
     CDS             <1..319
                     /locus_tag="VF058_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013859593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine dehydrogenase"
                     /protein_id="HEX6207095.1"
                     /translation="GAANNQLELPEHGDALHEAGILYAPDFVINAGGLINVADELIGY
                     DRERAMKKVEGIYRTLREVFRRSKTEGISPARAAEMMAAERIEKVSRVRLLWVPRRND
                     GWR"
     gene            384..1463
                     /gene="pdhA"
                     /locus_tag="VF058_01945"
     CDS             384..1463
                     /gene="pdhA"
                     /locus_tag="VF058_01945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX6207096.1"
                     /translation="MAQASDGGVRILALDGSVEDQEARDALDLKDEDLRQLYRFMVIT
                     RKVDLESTALQRQGELAVYPPLIGQEAAQIGSAYALGDDDFVFPSYREVGVAVARGID
                     LVDYMHFHRGTWHGGIPDPVGKRFGMISVPVGSQPLHATGWAMGAKLDGKAACAIAYF
                     GDGASSQGDVHEAMNFAGAFGAPVVFFCQNNGWAISVPIERQTAGEIYRRAEGYGFPG
                     VRVDGNDVLAVYRATREAAERARTESLPTLIEAITYRMGPHSTADDRSRYQPEEQIEA
                     WGARDPIERFRRFLDAEEAADDAFYEEVEEEGKARAAEVRKGIAGAPPRPVEDLFKWI
                     YTGDELPPHLARQRDEALAEEGDDA"
     gene            1456..>2276
                     /locus_tag="VF058_01950"
     CDS             1456..>2276
                     /locus_tag="VF058_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6207097.1"
                     /translation="MPEVTMGQALTQALRYSLEQDDRVLILGEDVGKLGGVFRITDGL
                     QAEFGEKRVFDTPLAESGIAGVSLGLTMAGWKPVAEMQFDAFSYPALDQVISHMAKWR
                     YRSAGTLSVPVVIRIPEGGGIGAAEHHSESPETYYVHTSGLKVVVPSTALDAFTLLVR
                     SIQDPDPVIFMEPKSRYWSKESGELSPDGLPIGAARTMREGTHATLIAWGAMVQRCLE
                     AAEAAEEDGVSVEVLDLRTLVPPDLEAMAASVRKTGRAIVVHEAPMILGFGAEVVA"
BASE COUNT          351 a          776 c          843 g          306 t
ORIGIN      
        1 cggggccgcc aacaaccagc tcgagctgcc ggagcacggc gatgccctcc acgaggcggg
       61 gatcctgtac gcgcccgact tcgtgatcaa cgcgggcggc ctgatcaacg tggccgacga
      121 gctgatcggc tacgaccgcg agcgcgccat gaagaaggtc gaggggatct accgcacgct
      181 ccgcgaggta ttccgccgct cgaagaccga ggggatctcc cccgctcggg ccgccgagat
      241 gatggccgcc gagcggatcg agaaggtcag ccgggttcgg ctcctctggg tcccgcgccg
      301 caacgacggc tggcgctgac cccgtgggcg cgcccgggcc gcgtgcccta tcctgagccc
      361 acccgggacc aggaggcacc ggcttggcac aggcatcgga cggcggcgtc cgcatcctcg
      421 ccctcgatgg gagcgtcgag gaccaggagg ctcgagacgc gctcgacctg aaggacgagg
      481 acctccgcca gctctaccgc ttcatggtga tcacgcggaa ggtagacctc gaatccaccg
      541 ccctccagcg ccagggggag ctggccgtgt acccgcccct gatcgggcag gaggcggccc
      601 agatcgggag cgcctacgcg ctcggcgacg acgacttcgt cttcccgtcc taccgggagg
      661 tgggcgtggc ggtggcgcgg ggcatcgacc tggtcgacta catgcacttc catcggggga
      721 cctggcacgg gggcatcccc gaccccgtgg gcaagcgctt cgggatgatc tcggtcccgg
      781 tggggagcca gcccctccac gccacgggct gggcgatggg cgcgaaactc gacggcaagg
      841 ccgcgtgcgc gatcgcgtac ttcggcgacg gggcctcgtc gcagggcgac gtccacgagg
      901 cgatgaattt cgccggagcg ttcggcgccc cggtcgtctt cttctgccag aacaacgggt
      961 gggccatctc ggtccccatc gagcgccaga ccgcgggcga gatctaccgg cgggccgagg
     1021 gctacggctt ccccggggtc cgcgtggacg gcaacgacgt cctcgcggtg taccgggcga
     1081 cgcgcgaggc ggccgagcgt gcccggaccg agtccctgcc cacgctgatc gaggcgatca
     1141 cctaccggat gggcccgcac tccaccgccg acgaccgatc acgctaccag cccgaggagc
     1201 agatcgaggc gtggggcgcc cgggatccga tcgagcggtt ccggcggttc ctcgacgccg
     1261 aagaggccgc cgacgacgcg ttctacgagg aggtcgagga ggagggcaag gccagggcgg
     1321 ccgaggtccg gaagggcatc gccggcgccc cgccccgccc ggttgaggac ctcttcaagt
     1381 ggatctacac gggcgacgag ctccccccgc acctggcccg ccagcgcgac gaggctttgg
     1441 ccgaggaggg cgacgatgcc tgaggtcacg atgggccagg cgctgacgca ggccctccgg
     1501 tactcgctgg agcaggacga ccgggtgctg atcctgggcg aggacgtggg gaagctaggc
     1561 ggcgtcttcc gcatcaccga tgggctccag gctgagttcg gcgaaaagcg ggtcttcgac
     1621 acgcccctgg cggaatccgg gatcgcgggg gtatcgctcg ggctgacgat ggccggatgg
     1681 aagccggtgg ccgagatgca gttcgacgcg ttctcctacc ccgcgctgga ccaggtgatc
     1741 tcccacatgg cgaagtggcg gtaccggtcg gcgggcacgc tcagcgtccc ggtcgtgatc
     1801 cggatccccg agggcggcgg catcggggcc gccgagcacc actccgagag cccggagacc
     1861 tactacgtgc acacctcggg cctgaaggtg gtcgtgccgt ccacggcgct cgacgcgttc
     1921 acgctgctcg tgcgctcgat ccaggacccc gaccccgtga tcttcatgga gcccaagagc
     1981 aggtactggt cgaaggagtc gggcgagctg agcccggacg gccttccgat cggcgcggcc
     2041 cggacgatgc gcgagggcac ccacgccacg ctgatcgcct ggggcgcgat ggtccagcgg
     2101 tgcctggagg ccgcggaggc ggccgaggaa gacggcgtct cggtcgaggt gctcgacctg
     2161 cggacgctgg tgccgccgga cctggaggcg atggcggcct cggtgcggaa gaccggccgg
     2221 gccatcgtcg tccacgaggc cccgatgatc ctggggttcg gggcggaggt cgtggc
//
LOCUS       DASUTW010000058         7910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_665344, whole genome shotgun sequence.
ACCESSION   DASUTW010000058 DASUTW010000000
VERSION     DASUTW010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7910
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665344"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VF058_01955"
     CDS             complement(<1..815)
                     /locus_tag="VF058_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015108491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX6207098.1"
                     /translation="MTSLRVAIVGHVEWVEFVRVDRVPSAGEIAHGLEGWEEAGGGGG
                     VSAVQLAMLAGSSTLFTALGDDEVAGKARRSLEGSGVRVEAASRGGPTRRAITMLDDL
                     GERTIITVGHRLSPTGSDALPWDDLGAADGVFFTAGDEEALRAARRARVLVATSRVLE
                     EIRRWRVPIDVLVGSARDPRETFDVGDLDAPPRLAVLTEGARGGRCWTPDEGWSRYEA
                     APLPGPGGDAYGAGDSFAAGLTYGLAKGLAPEAALAVAARCGAAARARTGVSGR"
     gene            complement(823..2043)
                     /locus_tag="VF058_01960"
     CDS             complement(823..2043)
                     /locus_tag="VF058_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005271362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX6207099.1"
                     /translation="MRRLTPLLAALAIAGVVAPPVTASPREPVRLATFNASLNRNVEG
                     QLIVDLSTPANAQARTVAEIIQRTRPDVLLINEFDFDAGGVALELFQENYLSVSQNGA
                     APIEYPYAFTAPSNTGIPSGHDLNNNGAVGGPDDAFGFGFFPGQFGMALYSRHPILEN
                     EIRTFQMFLWKDMPGALLPDDPATPEPADWYSEEELEIFRLSSKSHWDVPVSIGGSVV
                     HVLASHPTPPVFDGPEDRNGTRNFDEIRFWADYVGPGRARGAYIYDDDGERGGLKPGE
                     RFVIMGDENSDPLDGDSIPGAIQQLIEHPLIEDPLPTSEGAVQASELQGGANLTHRSD
                     PAFDTADFSDAAPGNLRADYVLPHKALKVVDAAVFWPRNDQPFFDLVGVFPFPSSDHR
                     LVWTDLGIPGAARR"
     gene            complement(2110..2490)
                     /locus_tag="VF058_01965"
     CDS             complement(2110..2490)
                     /locus_tag="VF058_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine synthase"
                     /protein_id="HEX6207100.1"
                     /translation="MIVRTVEDVAGTPAQVSGGLWTSTRLLLRDDGLGFTMTETVIEP
                     GMDEELWYKHHLEACFCVEGEAEIEDLETGEHHEIRPGTLYALNRHDRHRLRTRTRVR
                     LICVFSPALRGDEIHDEDGSYLLE"
     gene            2612..3298
                     /locus_tag="VF058_01970"
     CDS             2612..3298
                     /locus_tag="VF058_01970"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6207101.1"
                     /translation="MYEDRWSSRRVVQVRPHLWRRLEPRLGDGRILEIGSGLRPTAPA
                     RGSFFVESSPRAVRALRRAGGRAVQTGGWELPFRDGAFDAVFALELLEHVEEDERMLA
                     EIARVLRTGGTALVSVPLHMSLWSAIDDACAHVRRYEPEELLEKMGAAGLSPEGYLTR
                     PAQVRPGLARLGTGVMSRLPRLSNWSLQHVVFPIASGWARAFGRVRWAEPRTPAPEGA
                     GGMTVLAAKG"
     gene            3443..3643
                     /locus_tag="VF058_01975"
     CDS             3443..3643
                     /locus_tag="VF058_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207102.1"
                     /translation="MTSKMTAVGDASLTGWPRRLGDPIARRVSSRTRFSAEQVRAFLG
                     FALLAFTLYSLFKRARRATKRA"
     regulatory      3679..3882
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3994..4917
                     /locus_tag="VF058_01980"
     CDS             3994..4917
                     /locus_tag="VF058_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020515120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6207103.1"
                     /translation="MRRRTGILVLTLALMAAACGDNPVAGPSPSPQRGSFPVTVEAVG
                     GPVTMENRPERIVSLSPTATEMLFAIGAGDQVVAVDDQSTFPPEAPITDLSGFEPNVE
                     AIAGYEPDLVVIANDISDLVGSLEGLDIPVLLQPAAEDLEGTYEQLGQLGELTGRQAG
                     AAEVVGEMRRRVEELLEGVPEWLQAPTYYYELDPTFFTVTSDTFVGQIFAVVGLQSIA
                     DEAEGAGSGYPQLSSEFIVAANPDFIFLADTKCCGESAETVAARPGWDRIEAVQEGNV
                     VALDDDVASRWGPRVVDLLEVVVEAVSGAGA"
     gene            4917..5975
                     /locus_tag="VF058_01985"
     CDS             4917..5975
                     /locus_tag="VF058_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6207104.1"
                     /translation="MATATAEVRFRPTRLRPGVLGMALGVLAVAGLAGVLIGPVGLSP
                     QGVLLELLDRVPFARVDSGLAEREQAILWQIRLPRVVVGGMVGAALSMAGAAYQGVFR
                     NPLADPYLLGAAAGAGLGATLAVAYLPSGGGPVGPLPAAAFLGALVGVGAAYGLGRSV
                     GRSRTSGTLILAGVAVASFLTAVQTYFQQREDDTLRQVFAWILGTLGTVTWREAALMA
                     PYLVVGGVVLLLHRRLLDVLSLGDEEAESLGVRAGRVRLVIVVAASLATAAAVAVSGL
                     IGFVGIIVPHAVRLAFGGSYRLVLPLSIAFGAAFLVLTDLAARTLLAPAEIPIGVVTA
                     FFGAPFFLAVLRRSKGMS"
     gene            5972..6784
                     /locus_tag="VF058_01990"
     CDS             5972..6784
                     /locus_tag="VF058_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020522441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6207105.1"
                     /translation="MIGLGLRSVTAAYDGTPALRGVDLEVASGSWTGLIGPNGSGKTT
                     LLRAVAGLMPHGGAVLLGGEEVARMPRRVAARLVAYVPQRPVVPEPMPVIDYVLTGRT
                     PYISYLGSERASDLEVAARVLARLELEDLAERPLGTLSGGELQRAIIGRALAQGAPVL
                     LLDEPTSALDVGHQQQALELIDALRREEGLTVVAAMHDLTLAGQFAERLVLLDRGAVA
                     AEGPVREVLTESTIGRHYGARVRILDDDGGGVVVIPVRDAVVGPAGEREEPA"
     gene            6781..7338
                     /gene="cobU"
                     /locus_tag="VF058_01995"
     CDS             6781..7338
                     /gene="cobU"
                     /locus_tag="VF058_01995"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019009758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEX6207106.1"
                     /translation="MSLTLLIGGARAGKSGLALALAERAGAPVSFVATARAIDEEMAE
                     RIERHRAERPSGWVTVEAPVDLRGALGRVPEGATAIVDCLTLWVSNLLEDGCDDEEVV
                     AAAEAAAEAAGARDGRTIAVTNEVGAGIVPMHPLSRRYRDLLGRVNAIWASRASRALL
                     VVAGRVLPLEEGWALLEDPGEAGRG"
     gene            7331..>7910
                     /locus_tag="VF058_02000"
     CDS             7331..>7910
                     /locus_tag="VF058_02000"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF014347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEX6207107.1"
                     /translation="MDDPIRELERALAGLPGPDEAARAAAADRAARVLRPKGALAGLD
                     EVAVWLAGWRGAPPRVEAPACIVFAADHGVAAEGVSAYLPEVTRSVLAALEEGVATAP
                     VLAAEMGVSLSVVDVGVDRPTGNLLVEPAMGEERFGEAIAAGRRAVAEADGCDLLVLG
                     EMGIGNTTAAAAVAATLFGGPVEEWTGRGTGVD"
BASE COUNT          971 a         2711 c         3085 g         1143 t
ORIGIN      
        1 cggccgctca cgcccgtccg ggcgcgagcg gccgcgccgc accgggcggc caccgccagg
       61 gccgcctccg gggccagccc cttcgccagc ccgtaggtca gccccgcggc gaaggagtcg
      121 ccggctccgt aggcgtcccc ccccggcccc ggaagcgggg cggcctcgta ccggctccac
      181 ccctcgtccg gggtccagca gcggccgccg cgggcgccct cggtcaggac ggcgagcctc
      241 ggaggcgcgt cgaggtcgcc cacgtcgaag gtctcccgcg ggtcgcgggc gctccccacg
      301 agcacgtcga tcggcacccg ccagcgccgg atctcctcga gcacgcgcga cgtcgccacg
      361 agcactcggg cccggcgcgc ggctcggagc gcctcctcgt cccccgcggt gaagaagacg
      421 ccgtccgccg ccccgaggtc gtcccacggc agggcgtccg agcccgtcgg cgagaggcga
      481 tggcccacgg taatgatcgt ccgctccccg agatcgtcca gcatcgtgat ggcgcggcgg
      541 gtcggccccc cgcggctcgc ggcctcgacc cggacccccg acccttccag actcctgcga
      601 gccttgcccg ccacctcgtc gtcgcccagg gccgtgaaga gggtggagga gccggccagc
      661 atggcgagct gcaccgcgga gacgccgcct ccgccgccgg cctcctccca gccctcgagg
      721 ccgtgggcga tctccccggc cgaggggacg cggtcaacgc gcacgaactc cacccactcc
      781 acgtggccca cgatcgccac gcggagcgag gtcacccgct cgtcagcgac gcgcggctcc
      841 ggggatcccg aggtccgtcc agaccagccg gtggtccgag ctggggaagg ggaagacccc
      901 caccagatcg aagaacggct ggtcgttccg gggccagaac accgcggcgt ccaccacctt
      961 cagggccttg tgcggcagca cgtagtcggc ccgcaggttg ccgggcgccg cgtcgctgaa
     1021 gtcggccgtg tcgaacgccg ggtcgctccg gtgcgtcagg ttggccccgc cctggagctc
     1081 cgaggcctgc acggccccct cgctcgtggg cagcgggtcc tcgatcagcg gatgctcgat
     1141 cagctgctgg atcgcccccg ggatgctgtc cccgtcgagc gggtcggagt tctcgtcgcc
     1201 catgatcacg aaccgctcgc cgggcttcag cccgccgcgc tcgccgtcgt cgtcgtagat
     1261 gtaggcgccg cgagccctcc ccggcccgac gtagtccgcc cagaaccgga tctcgtcgaa
     1321 gttccgcgtg ccgttgcggt cctcgggccc gtcgaacacc ggcggggtcg ggtggctcgc
     1381 cagcacgtgc acgaccgacc cgccgatcga gaccggcacg tcccagtggc tcttcgagga
     1441 cagccggaag atctccagct cctcctccga gtaccagtcg gccggctcgg gggtggccgg
     1501 gtcgtcgggc aacagggcgc ccggcatgtc cttccacagg aacatctgga acgtgcggat
     1561 ctcgttctcg aggatcgggt ggcgcgagta gagcgccatc ccgaactgcc cggggaagaa
     1621 cccgaacccg aaggcgtcgt ccggcccgcc cacggccccg ttgttgttga ggtcgtgccc
     1681 ggaggggatc cccgtgttcg aaggagccgt gaacgcgtac gggtactcga tcggcgcggc
     1741 cccgttctgc gacacggaca ggtagttctc ctggaacagc tcgagcgcga ccccgcccgc
     1801 gtcgaagtcg aactcgttga tcagcaggac gtccggccgc gtgcgctgga tgatctctgc
     1861 gaccgtgcgc gcctgggcgt tggccggtgt ggacagatcg acgatcagct gcccctcgac
     1921 gttccggttg agcgaggcgt tgaacgtggc caaccggacc ggctccctcg gcgacgccgt
     1981 gaccggcggg gccacgacgc cggcgatcgc cagggccgcc agcagaggcg tgaggcgtct
     2041 catgagctcc cccttccgtt gggagggcgc catgctatct cgagcgggtc ccgaagctca
     2101 ccttcgcgat cactccagca ggtacgagcc gtcctcgtcg tggatctcgt ccccgcggag
     2161 ggccgggctg aacacgcaga tcagccggac cctggtccgc gtccgcagcc ggtgccgatc
     2221 gtggcggttg agggcgtaga gcgtcccggg acggatctcg tggtgctcgc ccgtctcgag
     2281 gtcctcgatc tcggcctccc cctccacgca gaagcacgcc tccaggtgat gcttgtacca
     2341 gagctcctcg tccatgccgg gctcgatcac cgtctcggtc atcgtgaagc ccagcccgtc
     2401 gtcgcgcagg agcagcctcg tgctcgtcca cagcccgccc gagacctggg ccggcgtccc
     2461 cgccacgtcc tcgaccgtgc gaacgatcat gatcccctcc ttcgtccgcg gaagcctagc
     2521 gcgaggcggg tagcagcgga gcgaacgggt atcttgatgg ccgatgaaga cgtcaactcc
     2581 tacgtggggc cgacctcgcg acggcgtccg cgtgtacgag gatcgatgga gctctcgccg
     2641 ggtggttcag gtgcggccgc acctctggcg gcgcctcgag ccgcggctcg gcgacggccg
     2701 gatcctcgag atcggctcgg ggctgaggcc cacggcgccc gcccgcggct ccttcttcgt
     2761 agagagctcg ccgcgtgccg tccgcgccct gcggcgggcc ggggggcgcg cggtgcagac
     2821 cggcgggtgg gagctcccgt tccgggatgg agccttcgac gcggtcttcg ccctcgagct
     2881 cctcgagcac gtggaggagg atgagcggat gctggccgag atcgcccggg ttctgcggac
     2941 cgggggcacg gccctggtct cggtcccgct ccacatgtcg ctgtggtcgg cgatcgacga
     3001 cgcgtgcgcg cacgtccggc gatacgagcc cgaggagctg ctggagaaga tgggcgcggc
     3061 cggcctgagc cccgaggggt acctcactcg tcccgcacag gtgcgcccgg gcctggcgcg
     3121 cctggggacc ggcgtgatgt cgcggctccc caggctctcg aactggtcgc tccagcacgt
     3181 ggtgttcccg atcgcctcgg ggtgggcaag ggccttcggg agggtccggt gggccgagcc
     3241 ccggaccccg gcgcccgagg gtgccggcgg catgaccgtc ctggccgcga aggggtagcg
     3301 aaaacggaac cgcgagagtc cgaagactcc cgcggttcca aggcgatttg tacaccgaac
     3361 ccggtctccg gcgcaagacc cgaccccgct tttgtcactc tttcttcggg ttgaggggcg
     3421 cggcttcggg aagaacgagg tcatgacctc gaagatgacc gcggtgggcg acgcctccct
     3481 gaccggatgg ccccgcaggc tgggcgatcc gatcgcgcgg agggtgtcct ctcgcacacg
     3541 gttctcggcc gagcaggtcc gggccttctt gggcttcgcc ctgctcgcct tcaccctcta
     3601 ctccctgttc aagcgggctc gccgggcgac gaagcgggcc tgagctcggc ttgtcacccc
     3661 ccgcctcgtg cgcgagaatc gggggtgacg gtccgaggtg gcgacggcgg ggacggctcc
     3721 cccgccggag ctgaaaaggg aagccggtga gagtccggca cggtcgcgcc actgtgaccg
     3781 gggagcgacc ccgagggatg ccactggggc tcaggccctg ggaaggctcg gggaagcgaa
     3841 gatccggaag tcaggagacc tgcctcggaa gggagcaccg tccctcgcgt ctcgggggcg
     3901 gcccacgcgg cccccgctga gggggcctgc ttcgttcccg ggcctcgagg gacggccgac
     3961 gcggccgcgc tcgacggccg ggaaggaggc acgatgcgtc gccggaccgg gatcctggtc
     4021 ctgaccctgg cgctgatggc ggccgcctgt ggcgacaatc ccgtggcggg gccctcgccc
     4081 tcaccccaga ggggatcgtt ccccgtcacg gtcgaggctg tgggcggacc cgtgacgatg
     4141 gagaatcggc ccgagcggat cgtctcgctc tcgcccacgg ctacggagat gctgttcgcg
     4201 atcggggcgg gagaccaggt cgtagccgtg gacgaccagt cgaccttccc gcccgaggcc
     4261 ccgatcaccg acctctccgg cttcgagccg aacgtcgagg cgatcgccgg gtacgagccc
     4321 gacctcgtgg tgatcgcgaa cgacatcagc gatctcgtcg gctcgctgga ggggctggac
     4381 atcccggtgc tgctgcagcc ggcggccgag gatctggaag ggacgtacga gcagctgggg
     4441 cagctcggcg agctgaccgg gcgccaggct ggcgcggccg aggtcgtggg ggagatgcgc
     4501 cggcgggtgg aagagctcct ggagggggtc cccgagtggc tgcaggcgcc gacctactac
     4561 tacgagctcg acccgacctt cttcacggtg acctccgaca cgttcgtggg gcagatcttc
     4621 gccgtggtcg gcctccagag catcgccgac gaggccgagg gggcggggag cgggtacccc
     4681 cagctcagct cggagttcat cgtcgcggcg aacccggact tcatcttcct ggcggacacg
     4741 aagtgctgcg gggagtcggc cgagacggtc gcggcgcgcc ccggatggga ccgcatcgag
     4801 gccgtgcagg aagggaacgt ggtggctctc gatgacgacg tggcctcgcg gtgggggccg
     4861 cgagtggtgg atctcctcga ggtcgtggtc gaggccgtgt cgggggccgg ggcctgatgg
     4921 cgacggcgac cgcggaggtg aggttccggc ccacccgcct gcggcccggc gtgctgggga
     4981 tggcgctggg ggtccttgcg gtggcggggc tggcgggcgt gctgatcggc ccggtggggc
     5041 tctcgcccca aggcgtcctc ctggagctgc tggatcgggt ccccttcgcc cgggtggact
     5101 cggggctcgc cgagcgggag caggcgatcc tgtggcagat ccggctccct cgggtcgtgg
     5161 tcggggggat ggtgggggcg gccctttcga tggccggcgc cgcgtaccag ggcgtgttcc
     5221 ggaacccgct ggccgacccc tacctgctcg gggccgccgc cggggcgggc ctcggggcca
     5281 cgctcgccgt ggcctacctg ccgtcggggg gtggccccgt cggcccgctc ccggcggcgg
     5341 ccttcctcgg ggcgctcgtg ggcgtgggcg ccgcctacgg gctgggccgc tcggtgggtc
     5401 ggtcgcgcac gagcgggacc ctgatcctgg cgggcgtggc cgtggcctcg ttcctcaccg
     5461 cggtgcagac gtacttccag cagcgggagg acgacacgct gcgccaggtg ttcgcctgga
     5521 tcctggggac gctgggcacc gtgacctggc gtgaggcggc cctgatggcc ccctacctcg
     5581 tggtgggggg cgtcgtgctg ctgctgcacc ggcgcctgct ggacgtgctg tcgctcggcg
     5641 acgaggaggc cgagagcctg ggggtccggg ccggccgggt gcggctggtg atcgtggtgg
     5701 cggcaagcct ggccacggcg gcggcggtcg cggtcagcgg gctgatcggg ttcgtgggga
     5761 tcatcgtccc ccacgcggtg cgtctggcgt tcgggggctc gtaccggctg gtgcttcccc
     5821 tgtccatcgc gttcggcgcg gccttcctcg tgctgaccga tctcgccgcg cgcaccctcc
     5881 tggctccggc cgagatcccc atcggcgtcg taaccgcctt cttcggggcg cccttcttcc
     5941 tcgcggtgct ccgtcgctcg aaggggatgt cgtgatcggc ctcgggctcc gatcggtgac
     6001 ggccgcctac gacgggaccc cggcgctccg gggggtcgac ctcgaggtcg cttccgggtc
     6061 gtggacgggg ctgatcgggc cgaatggctc ggggaagacc acgctgctcc gagcggtggc
     6121 cggcctgatg ccgcacgggg gagcggtcct cctgggaggg gaggaggtgg cgcggatgcc
     6181 tcgccgcgtg gccgcgcgcc tcgtggccta cgtgccccag cgccccgtcg tgcccgagcc
     6241 gatgcccgtg atcgattacg tgctgacggg gcggaccccg tacatctcgt atctggggag
     6301 cgagcgggcc tccgacctcg aggtggcggc tcgggtgctg gccaggctgg agctcgagga
     6361 cttggcggag cggcccctgg gcacgctctc gggaggagag ctccagcggg cgatcatcgg
     6421 gcgtgcgctg gcgcaggggg cgccggtgct gctgctcgac gagcccacga gcgcgctgga
     6481 cgtggggcac cagcagcagg ctctcgagct gatcgacgcc ctgcgacggg aggaggggct
     6541 cacggtcgtg gccgcgatgc acgacctgac gctggcgggg cagttcgcgg agcgtctcgt
     6601 cctgctcgat cgaggggccg tcgccgcgga ggggcccgtg cgggaggtcc tgaccgagtc
     6661 gacgatcggc cggcactacg gggccagggt ccggatcctg gacgacgacg gcgggggggt
     6721 ggtggtgatc cccgtgaggg acgccgtcgt cgggcccgcg ggtgagcggg aggagccagc
     6781 gtgagcctga cgctgctgat cggaggggcc cgcgccggga agtccggcct ggccctggcg
     6841 ctggcggagc gggccggggc gccggtgagc ttcgtggcca cggcccgggc gatcgacgag
     6901 gagatggcgg agcggatcga gcggcaccga gccgagcggc cgtccgggtg ggtgacggtc
     6961 gaggcgccgg tggatctgcg gggagccctg gggagggtgc cggagggcgc gacagcgatc
     7021 gtggactgcc tgacgctctg ggtctcgaac ctcctcgagg acgggtgcga cgacgaggag
     7081 gtggtggccg cggccgaggc cgcggccgag gcggccgggg cccgggacgg ccggacgatc
     7141 gcggtgacca acgaggtcgg ggccgggatc gtccccatgc atccgctgtc ccgccgctat
     7201 cgggatctgc tggggcgagt gaacgcgatc tgggcgtcgc gggcgtcgcg ggcgctcctc
     7261 gtggtggcgg gtcgtgtgct gcccctcgag gaggggtggg cgctgctgga ggatcccggg
     7321 gaggccggcc gtggatgacc cgatccgtga gctggagcga gcgctggcgg gtctcccggg
     7381 cccggacgag gcggcacgag cggcggcggc ggacagggcg gcccgcgtcc tccggccgaa
     7441 gggggcgctg gccgggctcg acgaggtggc cgtgtggctg gccgggtggc ggggcgcgcc
     7501 cccgcgtgtg gaggcgcccg cctgcatcgt gttcgccgcc gaccacggcg tggcggccga
     7561 gggggtgagc gcttacctgc ccgaggtaac gcgctcggtg ctggcggcgc tggaggaggg
     7621 tgtggccacg gcccccgtcc tggccgcgga gatgggggtg tcgctgtcgg tggtggacgt
     7681 cggggtggac cggccgacgg ggaacctgct cgtggagccg gcgatgggag aggagcggtt
     7741 cggggaggcg atcgccgccg gtcggcgggc ggtcgcggag gcggacggat gcgacctgct
     7801 cgtgctcggc gagatgggga tcgggaacac gaccgcggcc gcggcggtcg ccgcgaccct
     7861 gttcggcggc ccggtggagg agtggacggg gcgggggacg ggggtggacg
//
LOCUS       DASUTW010000059         2761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_276754, whole genome shotgun sequence.
ACCESSION   DASUTW010000059 DASUTW010000000
VERSION     DASUTW010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276754"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..>2761)
                     /gene="ileS"
                     /locus_tag="VF058_02005"
     CDS             complement(49..>2761)
                     /gene="ileS"
                     /locus_tag="VF058_02005"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX6207108.1"
                     /translation="WTMDPDYVQSVWWSLKELHRRGLLAEDFKIVPWCPRCETPLSDH
                     EVAMGYAQVTDPSVFVRFTVTEGDLQDTHLVGWTTTPWTLISNTGLAVHPDERYAVVE
                     RDGERLVVAEARREQVLGEEAVVVETLPGERLVGLRYAPLFDTVAGEGTHRVVGADFV
                     SMEDGTGIVHLSPAFGAEDLEIGRREGWPVVNLVDDRGRFTDEAPELVRGRFIKEADP
                     GITADLESQGLLVSSGTLEHTYPLCWRCSTPLIYYARTSWYVLTTRRKADLLAANEKV
                     GWYPEHIKHGRYGNWLENNVDWALSRARYWGTPLPVWRCGEGHDTVVGSLRELSELAG
                     RDVTSVDPHRPDIDEVTIPCPECGGEARRIPDVIDAWYDSGAMPFAQWGYHPDLGRGE
                     EVFERRFPADFICEAVDQTRGWFYSLMAEGVLLFGESAYRNCLVLGLIVDKDGRKMSK
                     SLGNVIDPWTIVDAEGADALRWYLFTAGPAWQTRRIFPEAVRETLRRFLLTVWNTYAF
                     FVTYATIDDVDPTADPPPRAERPVLDRWILSRLHATIRAAREGLEGFDATSAARAIDA
                     FVDDLSNWYVRRARRRFWDPAGPDDDRDKAAAFATLYECLATMATLLAPFTPFVSEEL
                     HQNLVRGTEPEAPESVHLADYPEADPEAIDPALEEAMALARTAASLGRAARTEAKARV
                     RQPLRRAVVHVPGDPEALRPLLHIVAEELNVKEVELASTAEELSGWTAKPSFRELGPR
                     LGKRVQEVAAGLEGDDGTLAGRLAAGETVELPLPSGPVELAPGDVELRRRTREGWGVA
                     SEGGVTVALDLDLDESLRLEGLAREVVRAVQDARKAAGLEVADRIELGLSAAGDVGRA
                     IEAHARWIEGEVLAVRTSGAGDLPDGHRASVQVEGEPIEIALRRAGG"
BASE COUNT          371 a         1074 c          941 g          375 t
ORIGIN      
        1 gatcggctca tgacgatgct cggagccgcg cccccggccg ccctccgcct atccgcccgc
       61 ccggcgtagc gcgatctcga tcggctcccc ctccacctgc acggacgccc gatggccgtc
      121 tgggagatcg ccggcccccg aggtccgcac ggcgaggacc tcgccctcga tccacctcgc
      181 gtgcgcctcg atcgcccgcc ccacgtcccc ggcggccgag agccccagct cgatccggtc
      241 ggccacctcg aggccggcgg ccttccgggc gtcctggacg gcgcgcacga cctcgcgggc
      301 cagcccctcc agacgcaggc tctcgtcgag gtcgaggtcg agggccaccg tcacgccgcc
      361 ctcgctggcc actccccagc cctcgcgcgt gcggcgcctg agctcgacgt cgccgggggc
      421 cagctcgacc ggtcccgagg gcagcgggag ctccacggtc tcgccggccg ccagccgccc
      481 cgccaacgtc ccgtcgtcgc cctcgagccc ggcggccacc tcctggaccc gcttccccag
      541 ccgcggtccc agctcccgga agctcggctt cgccgtccac cccgagagct cctccgcggt
      601 ggaggccagc tccacctcct tcacgttcag ctcctcggcc acgatgtgca gcagcggccg
      661 gagcgcctcg gggtcgcccg gcacgtggac cacggcccgc cggagcggct ggcggacccg
      721 cgccttggcc tcggtccggg ccgcgcggcc cagcgaggcc gccgtccggg ccagcgccat
      781 ggcctcctcg agcgccgggt cgatcgcctc gggatcagcc tccgggtagt cggcgaggtg
      841 caccgactcc ggggcctccg gctccgtccc gcgcacgagg ttctggtgga gctcctcgga
      901 gacgaacggc gtgaagggcg ccagcagcgt ggccatcgtc gccaggcact cgtacagcgt
      961 ggcgaaggcc gcggccttgt cccggtcgtc gtcgggcccg gccgggtccc agaaccgccg
     1021 ccgtgcccgg cgcacgtacc agttcgagag atcgtccacg aaggcgtcga tcgccctcgc
     1081 cgccgaggtg gcgtcgaacc cctccagccc ctcgcgcgcc gcgcggatcg tggcgtggag
     1141 ccgggagagg atccaccggt cgagcaccgg ccgctcggct cggggcggcg ggtcggccgt
     1201 cgggtcgacg tcgtcgatcg tggcgtaggt cacgaagaac gcgtacgtgt tccacaccgt
     1261 gaggaggaaa cgccgcaggg tctctcgcac ggcctcgggg aagatgcggc gcgtctgcca
     1321 ggccggcccc gccgtgaaga ggtaccagcg cagcgcgtcg gccccctccg cgtccacgat
     1381 cgtccaggga tcgatcacgt tccccaggga cttggacatc ttgcggccgt ccttgtccac
     1441 gatgaggccg agcacgaggc agttccgata ggctgactcg ccgaagagga gcaccccctc
     1501 ggccatcagc gagtagaacc agccccgcgt ctgatccacc gcctcgcaga tgaagtcggc
     1561 ggggaagcgt cgctcgaaga cctcctcgcc gcgccccagg tcggggtggt agccccactg
     1621 ggcgaacggc atggccccgg agtcgtacca ggcgtcgatc acgtcgggga tgcggcgggc
     1681 ctcccccccg cactcggggc aggggatcgt cacctcgtcg atgtccgggc ggtgggggtc
     1741 gacggacgtg acgtcgcgcc ccgccagctc cgagagctcc cgcagcgacc cgacgaccgt
     1801 gtcgtgcccc tcgccgcagc gccaaacggg cagcggcgtg ccccagtagc gggcccggga
     1861 gagcgcccag tccacgttgt tctccagcca gttcccgtag cggccgtgct tgatgtgctc
     1921 cgggtaccag cccaccttct cgttcgcggc caggaggtcg gccttgcgcc gggtcgtgag
     1981 gacgtaccac gaggtccggg cgtagtagat caacggcgtc gagcatcgcc agcagagggg
     2041 ataggtgtgc tcgagcgtcc ccgaagagac cagcagcccc tgcgactcga ggtcggcggt
     2101 gatcccggga tccgcctcct tgatgaagcg gccccggacc agctcgggcg cctcgtccgt
     2161 gaagcgtccc cggtcgtcga ccaggttgac caccggccag ccctcgcgcc ggccgatctc
     2221 gaggtcctcg gccccgaagg cgggggagag gtggacgatc cccgtcccgt cctccatcga
     2281 gacgaagtcc gcccccacga cgcggtgcgt cccctctccc gccaccgtgt cgaagagggg
     2341 cgcgtagcgg agccccacga gccgctcacc cgggagcgtc tccacgacga cggcctcctc
     2401 gccgagcacc tgctcgcgcc gggcctccgc gaccacgagc cgctccccgt cccgctccac
     2461 caccgcgtac cgctcgtccg ggtggacggc gaggccggtg ttcgagatca gggtccacgg
     2521 cgtggtggtc cagcccacca gatgggtgtc ctggaggtcg ccctccgtga cggtgaaccg
     2581 gacgaacacg ctggggtcgg tgacctgcgc gtagcccatc gccacctcgt ggtcggacag
     2641 cggtgtctcg caccgcgggc accagggcac gatcttgaag tcctcggcga gcagccctcg
     2701 ccggtgcagc tccttcagcg accaccagac gctctgcacg tagtccgggt ccatggtcca
     2761 g
//
LOCUS       DASUTW010000060        40627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1832076, whole genome shotgun sequence.
ACCESSION   DASUTW010000060 DASUTW010000000
VERSION     DASUTW010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 40627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40627
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832076"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VF058_02010"
     CDS             <1..760
                     /locus_tag="VF058_02010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6207109.1"
                     /translation="PRGVSTARNRGASEARGDWLAFCDDDDLWAPDKLARQLAAARDG
                     DRPWAYGGAVHVSLDLRVLSVKLPPPPSELVAALPGWSVMPGGSSNAIVRRDAFDEVG
                     GWDDSLVNLADWDLWARLARRAPPASSRRPLVGYRIHPGNASADTGLILREARALEKR
                     YGAPVDRGKLHHYLAWVYLRSGRRRPAVGHLARAATSGELTGVAGTVAALARRRVAGW
                     IPALEPRESREASAWRLEAEAWISRLREAEPAPA"
     gene            832..2301
                     /locus_tag="VF058_02015"
     CDS             832..2301
                     /locus_tag="VF058_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207110.1"
                     /translation="MIRDPLPSIGIGLSLLAALLVASWLFVAIHHVDDRFNVGWVEGT
                     RIALVWEADAGHFYPPLYDGESFGGTRFMPIPILLHQGLAQLTGEYLASGKLLAYLTT
                     LVLLTLTYRVMRRLRCPRPASFAITAGVLVTAPGLLAATTAQGDALAVAMQLGAVAIV
                     APRPRDPAATIAAGALCSFAVLSKVSAIWAPVALGAWLLAERRRLTLLLFVVSFVLLL
                     SAQVLILQTLTEGRMLENLRTTLLAGVTGPAGILGSPLRLVNLLGGIPLLWAVLPVAV
                     IGGAIWGSEDSRPFYLSLLTAFLVVLVVLADEGTVHNHLLDVTVLSLLFIGILFGRTT
                     EVSPAVALVLGSLLLWSIGTSYLLELWPATREAGIEVVRGIRSSRYDPRPLGRVIRPG
                     ESLLSEDPYVAVALDRPPVVLDAWTLLRLGRASSVPADSLARRIEAREFDWIVLVRPI
                     EDEEWYSDVHFGTIVADAISGSYRIQRQASGYHVYVPRR"
     gene            complement(2325..3872)
                     /locus_tag="VF058_02020"
     CDS             complement(2325..3872)
                     /locus_tag="VF058_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207111.1"
                     /translation="MAAEPEQVGNRPMPVAGGPPAERVLAAALVGLTVLALTATIVVA
                     WVHADDRYRVDHVGGSRIAMAWYVNEGTLYPQLSDDGFYGGTRWMPLPVLLHAGAARV
                     TGEYLVSGKVLAYMTMATLILVTFLVIRRLGCPWPLAAALASLLLLTQAGFGLTMSLR
                     ADALPVLLQVSSVWLATSRPHRSATPVAMIVAALLAALALAAKLSAVWAPIAVVLWLF
                     RRNRRSVSWFVGGYVVWAVGLLGLFSALSGGRMLSNVLGLAGAGLAEGGLLRAPYRLV
                     LLAIDHATPIWFLMPAAAVAGWYAFRGRRDEEWLLALLAASAILVVVLADVGADWNHL
                     IDIAVLVLIVVGGLVGRTLPSLPYERVAGTAVVLMVLWVGVTGLVTVVLPEARHVVRL
                     ADPTTDPQLDPQPLRGLASSETPILSEDPYVPVSLGQRPVLLDPFMVERIVEVDREIQ
                     AELLRRIDAKEFELVVLLRRVENRDIAWWWEDFHLGPEIAAEIRDSYDYQLSRGPYHI
                     YAPAEAS"
     gene            3943..5178
                     /locus_tag="VF058_02025"
     CDS             3943..5178
                     /locus_tag="VF058_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207112.1"
                     /translation="MLRRSWWLLFVVFAVTRVGLIYVGVDPSIYGGRDHDIGSDVSIY
                     AGWADGIIEGDVPYRDEPIEYPPGSLPFIVAPALGKAITGLPYRAFFGAAMLAVDVLG
                     LIAVLRLVGHQVSRAGPWLWVLGLAALGPVVWTRLDLVPAVATLWALERIVARAWTSG
                     GVLLAFGAVAKVFPGFLFLPAAAAAPRRTPFLGAGAATALLLLAPFGVVLHPMLSSVA
                     GYHLGRGIQLESVWGGAMLLTGLAGAPVSVEKRFGAWETLGPGTEALGIASTVLAVGV
                     LAGSVAVAWRRRDEPLRLAGVWFATLALLLAVGPVLSPQFMVWLVAVGAGALAVGSDT
                     VRKAALFLLPLAVLTHLVYPISYDGLVAMEPVSVWLLTARNAMLAALAVWSTVGVVRD
                     RPTETHRADRGSRRGWEAP"
     gene            5501..7084
                     /locus_tag="VF058_02030"
     CDS             5501..7084
                     /locus_tag="VF058_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207113.1"
                     /translation="MLSTAEDQSLTSLVRDQYATNPNLAMRPTQVVYQAFLFRLFGLE
                     PLGYHLVNLGVLIAVAVLLSLVLRELRLPRGLAVAVPAVYALMPNYATARFWFASFGY
                     LLGMALYLLALYADLRAASSEHRRTVWTWKALALVTLTTAAFGYEVVIPLFLLNIGLA
                     EVVARRLPSRGFRARMGPAGTALLHGSTLAVAVAAGLYKASVAVGVGVGSSPLRYTLW
                     LGSSSVATNFGTHGVGLPHTVGWALPHAGIGGVAIAILVGLGIYRYLAVVRDPRSEGD
                     ALPFRGGWLRMGVVGLVVFALGYSIFLTTGRIAFSSTGIANRVATAGALGAAIVLVGA
                     VGWIASRLRSPRLRDRAFRAGIAALCLSGIVVVNGLASFWVQSWEEQREVLADLDAAF
                     PRLAPGSTVLLGGVCPYVGPAIVFESSWDLQGALRVRHGDKSVDGDVTSGVVSAEPQG
                     VVTQIYQVEVMHPYGARLFLYDRRAGTTTVLVDQSAARRALSTQVSGTDCPEGRPGAG
                     TIDLPFDVLFHWFRSRIGL"
     gene            7113..8420
                     /locus_tag="VF058_02035"
     CDS             7113..8420
                     /locus_tag="VF058_02035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015183970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6207114.1"
                     /translation="MTDDQGSDRGDDRPAGASKRPTVGMLHWPDRFEDFHDRLGVTFE
                     SFRDEFTGSWVFNYVEALQRAGVRPVLYYASARIREPLRFTHRPTGAAVRVLPSPRLH
                     QKLRGAQERFGIRSASLRALGSYLATPLRPLVRALREDGCGAILCQEYEHPRFDVAVG
                     LGRLLRLPVFATFQGGEAPATRLEVPLRRLAVRRAAGLIVASAAQAERARRRYGLPSD
                     RIARIPNPIDVDRWRPIPQTAGRAELGIPADAPVVTWQGRVAIDQKGLDVLVEAWARV
                     CESHGEDAPLLLLVGHGQDAETLRAMLAGLPPHAVRWVDRFVHDREALWRYRAAADVA
                     VLPSRREGFPVAAVEAMACGLPVVAADALGVRDALGEGEEAAGVVVPREDPGALAAAL
                     ERVLAEPDLRRTLGERGRRRAEERFSLDVVGARLRDFLFGRRA"
     gene            8462..9292
                     /locus_tag="VF058_02040"
     CDS             8462..9292
                     /locus_tag="VF058_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6207115.1"
                     /translation="MGTGTREEWIENRPSRGLRLPDLRELWQYRELAYFLALRDLKVR
                     YKQAALGAGWAIFQPVVLTVVFTLVFTRFARVETAIPYPIFALSGLIAWTYVAGSLTR
                     STQILVANTPLVTKVYFPRLLAPVASALPGMVDLLLSLLVLAVVMPFYDVRPTWAILT
                     LPLWLAAMLVVVLGVSLLLGTLNVRYRDVTHGITLLVQLWIFVSPVAYPSDAVPEAWR
                     VLYFLNPVAGVLEGFRWALAGAAWPGSVVFLSLGTALVLLAAGVLYFQHAERRFADVI
                     "
     gene            9294..10535
                     /locus_tag="VF058_02045"
     CDS             9294..10535
                     /locus_tag="VF058_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6207116.1"
                     /translation="MHAIEIEGLSKRYLLGEDRPGGTLVEAMSRSARRLLRMRSRPRR
                     DEVWALRDVTLAVPEGEALGVIGRNGAGKSTLLKILSRITEPTAGVARTRGRVTSLLE
                     VGTGFHPELTGRENVFLNGAILGMRRREIERRFDEIVAFAGVERFIDTPVKRYSSGMY
                     LRLAFSVAAHLEPDIMVVDEVLAVGDAAFQAKCLGRMETAEREGRTVVFVSHNLEAIA
                     KLCPRTIWLDGGEIREEGDSPAVIDAYLSEEVTRASEETVAGDPGAAVVLRSVRVLGE
                     AGEPLETLLRDEPFHIELRFSVRTQVPGLDVAVWVANAFGTEVFREGWFDHSPDRPSE
                     PGEYVATLRVPPVLNVGEYTVGVWIGSPYETLVDAVVARFHLQGDVKNRPSRSVVLHL
                     PWEVRRISGADDPTPAPHPRR"
     gene            10532..11728
                     /locus_tag="VF058_02050"
     CDS             10532..11728
                     /locus_tag="VF058_02050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6207117.1"
                     /translation="MKVLLSALACEPGKGSEPEVGFRAMLAAAREHEVWVLTASQSIP
                     ALERALRSDPRAERIHLEGIPFGVDAETIVHLNAVGYHRRYEEWQRLAADRSLELEAR
                     VGFDVVHHVTLASYWTRAGVAAVRKPLVWGPIGGGVDPPLRLVPELGLRGMVEAAARV
                     VGRPLIARLPPIRRTQRVASLILAQNPGTGRRLRSRGTVKLLSNALAVELDGVPIPKK
                     RTTDLLFVGRLLPWKAPILALRALRHLRHPRAVLRFLGDGPEQSRLEQTARKWGLSER
                     VQFEGWLPRDDVLSAMASAGALLHPAVHEEAGLCIAEALTLGTPVVCLDHGGPAEIVG
                     QWRGRPSALVTARGAEATARSIAAAVDRFLLHPPEPASGPVTAGTSFQDELLWSYERV
                     TAASPG"
     gene            11834..13444
                     /locus_tag="VF058_02055"
     CDS             11834..13444
                     /locus_tag="VF058_02055"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX6207118.1"
                     /translation="MSGDTAPLELRDYLALLWRRKWIIAAMTIVAVGAALFYSYRQIP
                     VYTSSAEVVVRPIQLEPAQSARGTTGFVNMETETRVANSGPVAERALDELEDSGIEPG
                     DVSASQIEESETLLFTATGLSPESAQASAQNWAEAYLGLRKEQALADLEEVRRPLEDQ
                     ITVLGQQAAVQRRIIQENQESNIAVATTAQQRYAAILAEVSLLRQDLNRYISAGSLHV
                     GEVLRAAPTPTAPANRNHVTTGGLALLVGMSLGVGVAYLRDRLDPRVRGTRELEVHSG
                     GSVLALIPSGKPLKDNLPITMAEPASELADAYIALGFRLLRMAGDREIRTLVVTSSLP
                     QEGKSSTVANLGVAMARSGKRIILVSADLRKPGLLTYFPQWNGRGLVDMVRDGTGMSE
                     ALSTTSVDNLWVLHGGSSLRGSSPLEIVSSDEMRRLLIELGSWADLVLIDTPPLLGAF
                     DASALAAFADGVLFVADGKRASGTTVEQARRELELSGANVVGVAVNRYDPRDYAGYAY
                     RAPYYRPENGRVLPPPELEQPQVSREER"
     gene            complement(13503..14414)
                     /locus_tag="VF058_02060"
     CDS             complement(13503..14414)
                     /locus_tag="VF058_02060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6207119.1"
                     /translation="MSAGRAVAEDVSVVIPTLGRDLLRGCLESIRSGATWPAELIVVD
                     QSQNPEVERWVDALTADGLEVVHLRSNQHGIAAATNRGLERVRTPYTAITHDDCRARE
                     DWIGQLAARVRNVGDAIVTGRVEPEGEGLVLTVITRAEPAVYKEPLIDGDVLFPANMG
                     FPIRLLERIGYLDEHPSLNVAGEDNDWAYRALRAGVPIIYEPGIVVGHLAWQDPSQRT
                     ALYRRYARGQGSFYGKHLRRGDLFIARRVLRDLVRAPWLLLRGVATGNRELFAMGVGE
                     VTGLLPGLVAGLGNRGHRVPRAPASSG"
     gene            complement(14411..15856)
                     /locus_tag="VF058_02065"
     CDS             complement(14411..15856)
                     /locus_tag="VF058_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207120.1"
                     /translation="MKVRRQEGVPEAAPEGRGARLGLLLVAVVFVAVSIPVVLRGAPL
                     ADDFSKCMEPQRVGLASALGDSVERLGAMRPAHFLEILVTTGVCQTIPFGVAIAVPLA
                     LTLAVALLLRGLLRDLGTPAPWPEVGAAVWLLQPLGTEAALWPAALHIPLGLALALWA
                     LRLHRAGRHGWAALAVLGAGLSVEQSLLALPLAAWLVAARDRRRAALVSTAVPVVMVL
                     AAFVLWPGEDPRLGVSLGERVASLFRDPSFYLEFPAVGLGLHSVPLAVAWAFPVSLAV
                     LAVGGLLGARAGPLILAGTATVWNGGFIRRAILAGAGLIVLVNVPVVLLVPRQGSPRL
                     FTPTWLVLSALVAVIGARVGFRRRAAVGAVAGVLAAGAVLSLALSVSVRLRSADVVEA
                     SSARIAAETEDGDVVAVCGVRRSVVEWAPRGAYAVHDLIYEWAARDALEYHTGRRATF
                     LLAGELWDRPCPGPDEVDLVVSFPELLEEAT"
     gene            complement(15853..16794)
                     /locus_tag="VF058_02070"
     CDS             complement(15853..16794)
                     /locus_tag="VF058_02070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6207121.1"
                     /translation="MTGPEVSAIIPTRDRAGPLSLALRSVLGQRAVDLEAIVVDDAST
                     DGTHDLLGGIADPRVRAVRNEAPAGVSAARNRGIEMARGAWVAFLDDDDLWAPEKLRH
                     QLDAAAATGRRWVYGGEVIVDERLRVVDGTPPPPPEEVVAALARHDAVPGGTSSVLVA
                     ADLLRRVGGFDPDLTTSEDWDLWIRLARAGPPACVPEPLVAVSVRRGRSSAMPRMLEE
                     LDVVARRHGIAVDLVRHYRWAAWEALRDRRRARAARYFWAAALRGDPGSIVRAVVALA
                     WPGVAERRARARGGSPSWTAGAEEWVRPLASGRGSGP"
     gene            complement(16791..17618)
                     /locus_tag="VF058_02075"
     CDS             complement(16791..17618)
                     /locus_tag="VF058_02075"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6207122.1"
                     /translation="MLLYHRLTEGEISGPQVIPSIPAELFRRQVEALAEVGRVVPLAD
                     LLAGADRGTPRFALTFDDDYPSHVVSALPILRELGLPATFFLSGRSLHGAGPYWFEAL
                     ERWVRERGVSEVAVRLEVPADLEAVILACEADERLRRVAEEEGRDEVRHLQPGDIAAL
                     ADAGMTIGFHTVDHAVLPRLPDQDLEEAVRAGRGELAEVVGRPLDLFAYPHGKADGRV
                     ARKVREAGYAAAWTGRPRPMHPREDRHLLGRWEPGRLPPDELLVRLSIRLSRADRIR"
     gene            complement(17732..18697)
                     /locus_tag="VF058_02080"
     CDS             complement(17732..18697)
                     /locus_tag="VF058_02080"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6207123.1"
                     /translation="MVASRTPDVSVVIPTRDRGDVLRLTLASALRQLDVEVEVIVVDD
                     GSASAPSVPGLDDPRVRLLREERSMGVSAARNRGINEARGAWVAFLDDDDLWAPDKLV
                     RQLQAARVSGRAWVYGGDVNVDADLRVLTGSLPPDPEEVVAALPRYNPVPTGASNVVV
                     SMEALVHVGAFDTTLKRTEDWDMWIRLARVGPPAWVPRPLVAYRFHPSNVPLETASIV
                     REPDLLAERYGIPVDRAAMQRRAAWAWLRAGRRGRAAGHYAMAVSMGDLRSVGRAAVA
                     LLHPAAGSDRVFRLIRSRPEDEEWRREAQRWLDDLAREALQRGRG"
     gene            complement(18702..20195)
                     /locus_tag="VF058_02085"
     CDS             complement(18702..20195)
                     /locus_tag="VF058_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX6207124.1"
                     /translation="MNRVEAATIILPAYDVGPAIGPVLREVAVAAYALRARGIDLDVL
                     LFDDGACGATATEVAEELGITLITFPAPPAGPALAYLEGFRRVVEEGRSDLVVTLDAN
                     GRHDPSQIPNLVDVLVDRRLDVVIGSRWAPGSGTPGMAFSRWALGKAANAAFRLITST
                     PRVADATTSFRVARTDVVREFRFGEIPVNSYSLHTAFVATAIAKGYRVGEASIIYRRP
                     IAGGGGLRMRDLGEFVSHLVALRTDVLRFRRRRLSPAGRAFPVEHFAAEEDLECLAAS
                     KHFFDWVLDDFGPYLRGRVLEVGAGAGTITRKLVERFPDMSIVALEPAENMFAQLEPY
                     AALTRRVEARRETLVDYGRRAFESFDAALYVNVLEHIEEDEKEVRMAAEALRPGGALL
                     VFGPALERLYSLLDHKAGHYRRYSLGRLRRLADAAGLRVVSLYYFDVVGVLPYLVVYR
                     WLHRTSIGSSTVWGYDRLMVPLSRWIQRFLRHPPLGKNVVMVAVKER"
     gene            20340..21443
                     /locus_tag="VF058_02090"
     CDS             20340..21443
                     /locus_tag="VF058_02090"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6207125.1"
                     /translation="MVPREEQPWSGILTVIVHLTAALRRQGHEVEVWQLHRWNDRYAA
                     QRRVLEAAEVVEIPVAVDAPAWRVGRIAASLAEKRSIDVVHLHGAFNIWTALVGRALR
                     RPYVFSPHSGYDPVSLRRSRFRKTVYGLAFERTLLRRAALLVGLTEVEVAQLRARGAR
                     GPAVVIPNGVAPPPEGVDPAAFREELGISPHAPLALFVGRLDVHRKGLDVLVEGIADA
                     PGWHLALVGPRFRDVERLEAAIRHPDLRDRVHLVGVRYGDALSQALAGATVFALLSRW
                     EGLPMALLEALSLGTPAVVSPAVEGLVPVAASGAGWVADDGRLVDVLREAASGAAARA
                     EAARALASRYDWDAVAREYGAAYRRARDRVATA"
     gene            complement(21433..22941)
                     /locus_tag="VF058_02095"
     CDS             complement(21433..22941)
                     /locus_tag="VF058_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207126.1"
                     /translation="MGPSVGDAFPRLPARLTRWLRGALILTSAGVVASWLFLAVVHLG
                     DRYQVRHRQGVWIALAQHLDAEGLYPQIFDGAHYGGVRYMPGPFVLHGLVARVTGEYL
                     TSGKLVALLATAALLVLLYVTLVRLGSPRPVAFGLTAAVVAGGAGLLGGTSVGGDVLP
                     VLFQLAAVALVLRTRSDARAVGAGALCTLALLSRFNALWAPLALAVWLWPRDRRRLLV
                     FAGSFVGLTVVSLGLLHVASDGRMYDNLVRFAAGGILSVGDAATAPLRFLDAVFEGAV
                     AQWVLLPVAAFGLLTLRRVEPSVFAWGLLFAAAILLVVFADIGTGSNQLIDLVVLMAL
                     VTGQLTAAFGERGDEVFASVVAVALVWVTFTGVAVHMREDLKEAGKIVLGAESPFRPD
                     PLAGELPAGATLLSEDPGLPALRGQTPVVFDPFMLNRIDRREPELVDDLVDRIREQEF
                     DALVLVETLEGNDGWWRDYHFGTRVVAAMREAYRWDRTFGGYHLYRPAQVTP"
     gene            23022..23954
                     /locus_tag="VF058_02100"
     CDS             23022..23954
                     /locus_tag="VF058_02100"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HEX6207127.1"
                     /translation="MRRETETVDSARADEPSRASAGSVVRDLLVTVRPRQWIKNLLVF
                     GAPATGRVLFEPAALASTTLAFLAFCLVAGGTYIINDIVDAPADRTHPVKRRRPIAAE
                     TISSRLAAVVALILLTGGLAVAFAGGGLPLVAVTGGYVALTLAYTYWLRDIVLLDIAA
                     IAGGFLLRAVAGGVATGVPLSEWFLLVASFASLFLAAGKRTAEYVRLGDDRGKHRPSF
                     DEYSETYLRFIQYSAATITIAAYALWSFEGETGGTAWSGLSIIPFALGIFRYGLLLDK
                     GHGAAPEDAILSDPPLLGLGVAWVLLVAVGVYVG"
     gene            23964..24941
                     /locus_tag="VF058_02105"
     CDS             23964..24941
                     /locus_tag="VF058_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEX6207128.1"
                     /translation="MPRALVTGGGGFLGSHLCERLLAEGWEVLAVDSLLTGNRDNLTG
                     LLGHDGFRFEQRDVTELEEDDPVLEEALDWVLHLASPASPVDYLEFPLDTLRVGSLGT
                     LRLLEVARAKGASFFLASTSEVYGDPLVHPQPETYWGNVNPIGPRSVYDEAKRFAEDA
                     TMAYRRQHNLKIRIVRIFNTYGPRMRRGDGRAVPTFIEQALRGEPLTVHGTGTQSRSL
                     CYVDDLIEGFYRLILSDQTGPMNVGNPEEIAIADLATTIRDIVGSPSEVVMVDRPVDD
                     PEMRRPDIELARRVLGWEPTVPLRDGLTRTIDWARTAWRSEVGSADDRP"
     gene            24938..25882
                     /locus_tag="VF058_02110"
     CDS             24938..25882
                     /locus_tag="VF058_02110"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX6207129.1"
                     /translation="MTRRTGILRISREMKASSPAFIVGEARSGTSILYRTLMKHTSFR
                     THEPNLVETEIFAHLRRVFMFGPDYPAPLLRYMLDDRKAYGAFLASIRPLRLVSALNI
                     PVNLLSRGPMTWTLRTNMAHLVLRSFFFHARLARGCRRLVEKTPTNTPYIPALSRIFP
                     QARLLYIHRHPVDVYSSYRRRGQADPDAAWARSLTPDAFCEMYAHSAALALDWAAGHH
                     NLLLVSYERFTGSPEVELRRVCEFLGEPFEAAVLEEPNPDPGRWKGDPLLWGPIVPVT
                     KRWQDYMTGDEAALIEHRLHGTMRRLGYDAVGPPAASG"
     gene            26316..27770
                     /locus_tag="VF058_02115"
     CDS             26316..27770
                     /locus_tag="VF058_02115"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX6207130.1"
                     /translation="MPGKGVETGSAPAQRPTLRLERRRTARTAGTALPLRRRARFGLV
                     VADAVALLGALAVMGSIRPEIQGLSVHILAPMAVAVVAQIVVFAAVGLYRLWRMPAWD
                     EVRRMVGATAIWVIVVAIATPWWSGAVSRSFLLYTWLIALAFLFPMRGVVRWALSRAK
                     TKEEAALRTAVVGPNGEAGSMVSGLSGSGEIAPVGYIAMNPGDTHSNGLPVLGTLQDL
                     GSIIVDKEIECLLLPATGLSEDEILRVSRACRRASIELKVWSELPDILGSRLAIQPMD
                     GRVALALEPVHFTSTQAALKRSFDLVTASLSLLVLLPFLALIGLAIRLTSRGPVLFRQ
                     PRVTKDGRVFTMYKFRTMVSELQEALEIDLTQPFFKMREDPRLTKVGRMLRSLSLDEL
                     PQLLNVILGDMSMVGPRPLPVEQIEANAEFFAPRHEVRGGITGWWQISGRSALDSRRA
                     LRRDLWYIENWSLGLDVYILIRTLGAVIARRGAW"
     gene            27781..29160
                     /locus_tag="VF058_02120"
     CDS             27781..29160
                     /locus_tag="VF058_02120"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX6207131.1"
                     /translation="MKIGVIGTGHVGLVACVTYAEMGHQVVGVDVDEERVDQLGRGIA
                     PFFEPGLVELLQRHLAGGSLSFSTAIADAVRGAEVVFICVGTPSRATGEANLLAVERV
                     AREVAVHSDQAAGGRLVLVQKSTVPAGTATRVRRALRMANRDAGARVSVASNPEFLRE
                     GQAIRDSLEPDRILVGADSVYAFEAMRRLYKSLIDNGTEYIETDVPTAELAKHASNAF
                     LALKISYANALARLCERSGADVTRVADIMGSDPRIGRAFLDAGMGYGGFCFPKDLQAF
                     EHLSDSLGYDFPLLREVARLNNEALVATLTKIKEGVWNLEDKRVALLGLSFKPGTDDV
                     RFAPALELAQRLLDEGATVVGYDPQAMTNAKQEVPGLELAADPYEAASGAHCLVLCTQ
                     WPEFRDLDLHHLRRVMTDPLVVDGRNLFDARQMAAAGFLYFSVGRPPGGLEIDLRDVG
                     ETQQADASS"
     gene            29198..30202
                     /locus_tag="VF058_02125"
     CDS             29198..30202
                     /locus_tag="VF058_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207132.1"
                     /translation="MTRAPERVPDPTREWATLPTSRAPRWFLPRRRGTATRRSFMIYH
                     PVTLRSRVGWEAARRLAAVGALELLRPSNMLPREVWEPVAGVIPQGGALAVARANHPG
                     RFLALVVGADGDLLAFLKVARDSVGSDVLERERRALETYGPLLPPPLFTPRVLDGQAG
                     SLLLEAIEWRSRAIPWGLPTDVAHAMGRFYAASVSEGSQGVTQGDFTPWNLLRAPHGW
                     ALVDWESAESGVPPFFDLFHFFVQSSAELRRPSKRAVIEGLDLRGWVGACVEAYSAGA
                     GVDPRAAKDLFGEYLRFSAARVSAGAPPRARRIRRELAARLGAVRTGAPQPDAAGGGP
                     "
     gene            30199..30852
                     /locus_tag="VF058_02130"
     CDS             30199..30852
                     /locus_tag="VF058_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207133.1"
                     /translation="MRSWSGVYVVLAGPDGSGKSTLASRLAAAARRSGRPARHLHWRP
                     GLLPQAGSLVGRAPGDPSTPHAEEPQGRVLSLLRLVYYWADFLLGTWLRVQPITARGG
                     LVVMERGWWDIAVDPRRYRLRVGARVIRALGRLLPAPDLVLVLRASPEILAQRKEELP
                     TSELARQNDRWDEIRFPQRIAKIVLDATRSQDDLVADAAAHMATVSGGDPVGAGGVE"
     gene            30855..32105
                     /locus_tag="VF058_02135"
     CDS             30855..32105
                     /locus_tag="VF058_02135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6207134.1"
                     /translation="MPRFLAFAYGCEPDKGSEPGAGWIWSRMLARIGETWVITRENNR
                     PSIEAALPSTPERDNLRFVYVDLPERARFWKRGMRGARLYYVLWQAAAVSRARRLVRE
                     SEFDAVWHLTWANAWIGSMAALLPRPFIYGPVGGGVRMHWGFTSVVGFRGTMYEVFRA
                     LARGTARYFNPIARLAWRKARLILVLNEDTRNWLPRRHRDRAVVFPHAVLDRPPRPTA
                     DSRSPGRNVALFAGRLVPWKGGALAIQALSLLPEWRLLFCGGGPDEARLKLLAARSDV
                     EERVDFLGWMTRDEVTSWMRKADVFLFPSLHDDAPFAVAEALAEGLPVVCLARGGAAA
                     IAGRGVPTASVASTVAGLAEAVRESSNVEVPAFPDIDSRAQRLAELLRERFPSWGIQV
                     PEETNHPEAIHGPTGLRGDPPAES"
     gene            32137..33522
                     /locus_tag="VF058_02140"
     CDS             32137..33522
                     /locus_tag="VF058_02140"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX6207135.1"
                     /translation="MISGLALAAAAGAYSVGHPQRALVFGPLFVLGLILLDLNGRKGT
                     LVDRFGWIPWAWVALLLVTDLRLINADPLAVAAGAANVQHLIQIAAYALVTVLVVRAR
                     STIIGLFPRPVPKGLLLAFPLVAVASAAWSLIPIYTVVRALQLVVLALLALLVVRIWA
                     SDPDAGEEIFARMLRLFVQVVSILAVIGLATAENLGDRFTWLGMHPGVTATYTGAALL
                     ILILGGRSWLRFPGYTYWPRLLLFGVVIYLAQTRSVLAGIVIGIGVGLWFKGRDKPIA
                     RYLGVAYYLIGGLLFVLATSLQLTGYLTRGETAESIASLSGRIPLWEFAIGQLESVQE
                     WALGFGYGSSRLVLLASPGFQWAGSAHSTWVEVLLGIGVVGFVATAATIVALVFTLSR
                     SQAFGRPNVIALSILAFFLMASPISEGMALPGIGFGFLALLHVPALGKRALRLPRHRP
                     DRARADVRTRG"
     gene            33594..34841
                     /locus_tag="VF058_02145"
     CDS             33594..34841
                     /locus_tag="VF058_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207136.1"
                     /translation="MADQALSSLTNFVIGFLAARGLSQSEFGAFAVVFAVFLIGLGVT
                     RAINSEPFVIRYSAVSADRWRHGATWATGTALAVGALGGVACLVAGIIAGGALGSGLV
                     AVGVALPGLMLQDTWRFTFFAARRGRSAFTNDAAWVLALAAGVALLAVADSWSVGSLV
                     LAWGGAGTLAGVIGILQAGVVPTPNRSLDWLRDQRDLAPRFLGEFTVTTLVSQITVFG
                     LGILAGLADVGALRAGTLLLGPLNVLFLGVGIVAVPEGVRALVSAKEKLLRVCRRLSI
                     TLASFALAFGTAMWLLPDRIGVALIRENWHAGHSVIIPLTLVVAFSGILLGAGIGLRS
                     LAAAKLSFRARLLVAPISLVAGLGGAVLAGAVGAAWGMAFAFVVGAIIWWRYFLKGLR
                     AHVVGPPDERAATDVYPVIHGDA"
     gene            34834..35748
                     /locus_tag="VF058_02150"
     CDS             34834..35748
                     /locus_tag="VF058_02150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6207137.1"
                     /translation="MRRLSILTPSFNYAWCIEDALKSVAQAAGFVPGGWAVEHVVVDD
                     ASTDESGEILERWRDRVVLERQTENQGQSRTLNRALALSSGEWIGWLNSDDFFLPLSL
                     ADASRAFDDEDADVIYGDVALLDGSARLLRLMTEHPFSLWTLRWWGTYLAVGTVFLRR
                     DLVSRLRWREDLSLLLDWDLWLRAAESGARFRYLPRPLAAVRRHPGQESRQDRPDRLT
                     EKATVRREHRLPSRPWMWRAAQRVAALTHGVQKLAAGGYAREQRAGAVRGRPMRWFED
                     PEARSTVDSLYELVYGGRSDGTLPSREG"
     gene            35748..36908
                     /locus_tag="VF058_02155"
     CDS             35748..36908
                     /locus_tag="VF058_02155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6207138.1"
                     /translation="MRILILHSRYRSGPASGENRVVEDEARLLSESGHDVEVWDPSVG
                     TPSGIRLLQAGARVVWGRAAAAEVRTRVARFRPDVVHVHNLFPSLSPSVLRTVTGRAP
                     LVMTLHNYRLHCLPGVFLRDGRVCEDCLGRTPWPGVLHGCYQGSVPASFALASSLVLH
                     RRLRTFDGVDLYLAISEFVRRKNVEGGLRGDRIIVKPHFVWGGEVRQGPGDYFLYLGR
                     LSPEKGVATLIEAWRVVEARLLVVGEGPEASALRERAPSNVEFRSTVPPEKARALLGS
                     ARAILVPSLSHEGAGRVVLEAYAAGVPALASRSGGLPEVVSDGETGLLLPVGDPHAWA
                     EGVSRLLSDAESERMGRAAFGLWQRAYSPQSGRGRLEDAYRSVVGTRATRDG"
     gene            36925..38112
                     /locus_tag="VF058_02160"
     CDS             36925..38112
                     /locus_tag="VF058_02160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6207139.1"
                     /translation="MRIAHFLLGRSNPESSNGVDKTVFFLSKAQAELGHDVSVLSISD
                     LPPIPVPGVTVGTFAPRRLPSLRPFDKEGRARPRNPFRLPDGMWDDMVGRHPHIVHFH
                     SVHIPEALWLADRLRRSEIPYCVSPHGGLLTTAQRHRRRLRKQLFSTLLERRYLRRAS
                     LIHAVSQAEAEELRRQNLGPPVVVAHNCIDASAVPDDVDPGPVIERFPQLQGKRVLGY
                     LGRLDTGPKGLDLLLRGWARIADPSNEVLLLVGPDWRGGRMRLERLASGLGVNGGVVF
                     APAVSGREKWAYFAAMDVFVAPSRWEAAPFTVLEAMATRTAVVATPASDPDGLIRASE
                     AGWVVQAEVEALARALKTVTQTSGEQLRLMGTKARRLIEREYRWNDAAEILTEAYEDA
                     TRG"
     gene            complement(38134..39000)
                     /locus_tag="VF058_02165"
     CDS             complement(38134..39000)
                     /locus_tag="VF058_02165"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6207140.1"
                     /translation="MRWRRSVKRGAVRVGRLFERPHRRVVLVYHSIHPSSWDFSSPPE
                     LFESHLEWLRDECEVVPLRDLIASRSGEPPAKPLVALTFDDGYEDNHAHAMPLLARHG
                     LPATFFVTAGLIDGDRDVRERFGTIVRRDPGDLRFMSWSQLREMTSAGMEIGNHTYSH
                     RNLARLAPAEVDEEVGRARSLLSEHLGTDVDLFAYPFGRPRVHFTPSVERIVEQHGHA
                     IGLAAMYRQVRASDPLLRIPRFFVDGDDVERLRDKVLGAYDVVGWWQEHTPLWMVRLV
                     SPELGRRSPAGA"
     gene            complement(39026..39832)
                     /locus_tag="VF058_02170"
     CDS             complement(39026..39832)
                     /locus_tag="VF058_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207141.1"
                     /translation="MSGHELTMPSAEWLPLLRQLTGSSESWGIWKNTDRALAGSGDVD
                     SAAPTRDHAELATLFYDWAEGQGLQWVIECVHAEGVLALIAVSESTWAQLDVAAESSF
                     RGTRLFTAEDLVPLMVMDQRGFRRIREGAEGLLLFLYKGIRWGGRRMGQGLEKYGVLQ
                     KMAGDWEGTEEAITRLGLPRAPMVRAIRAAARGRWERFPLIHAEAVMMMRGLAHPRFL
                     LSRAASRGWGMRQCDVLKAIGAAHRISVPTWLEDVRANHTVTALPASRRS"
     gene            39980..>40627
                     /locus_tag="VF058_02175"
     CDS             39980..>40627
                     /locus_tag="VF058_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018799555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6207142.1"
                     /translation="MVTGSGGLVGSEACLYFGDLGLDVVGVDNDMRATFFGDEASTAW
                     NVDRLRQQLGDSYRHHDLDVRDRDGVLDLFARHGSSIELVIHAAAQPSHDWAAREPFT
                     DFDINAVGTLNVLEATRLHAPEAVFIFTSTNKVYGDAPNRLPLQELEKRYEIEEDHPY
                     VDGISEDMSIDRSLHSLFGASKVAADVLVQEYGRYFGMRTACFRGGTLTGQLHSAT"
BASE COUNT         5914 a        14402 c        14016 g         6295 t
ORIGIN      
        1 cccccggggg gtgagcaccg ctcggaaccg gggtgcctcg gaagcccgcg gcgactggct
       61 cgccttctgc gacgacgacg atctgtgggc gcccgataag ctggcccggc agctcgcggc
      121 ggcccgggac ggggaccggc cgtgggcgta cggcggtgcg gtccacgtga gcctggacct
      181 cagggtgctc agcgtcaagc tgcccccgcc gccctccgag ctcgtggcgg cgctccccgg
      241 gtggagcgtg atgcccggcg gatcctcgaa cgcgatcgtt cggagggacg ccttcgacga
      301 ggtcggcggc tgggacgact cgctggtcaa cctggccgat tgggacctgt gggcccgtct
      361 ggcgcgccgg gccccaccgg cctccagccg ccggccactg gtcggctatc gcatccatcc
      421 ggggaacgcc tcggccgata ccggcctgat tttgcgcgag gcgcgggccc tcgagaagcg
      481 ctacggcgcc ccggttgacc gagggaagct ccaccactac ctggcatggg tctacctgcg
      541 gtccggacgg cgacgccccg cggtgggaca cctcgcccgg gcggccacgt ccggcgagct
      601 caccggcgtg gccggcacgg tcgcggccct cgcccgccgc cgggtagccg ggtggatccc
      661 tgcgctcgag ccgcgggagt cccgggaggc gtccgcctgg aggctcgagg cggaggcgtg
      721 gatctcgcgt ctccgggaag cggagccggc ccccgcgtga cggaacggat atggccgccc
      781 tccggcgagg acccgggaga tccttccgga ccacggcgat ggtgggccgc catgatccgg
      841 gaccctctgc cctcaatagg catcgggctc tcgcttcttg ctgctcttct cgtggcctcg
      901 tggctcttcg tggccatcca tcacgtggac gatcgtttca acgtcgggtg ggtcgaggga
      961 acgcggatcg cacttgtgtg ggaggccgac gcgggacact tctacccgcc cctctatgac
     1021 ggtgagtcgt tcggggggac acgattcatg cctatcccca tcctcttgca tcagggtctt
     1081 gcccagctga ccggagagta cctggcttcc gggaaactcc tggcgtatct cacaaccctg
     1141 gtcctcctga ccctgaccta ccgtgtcatg cgtcgcctga ggtgcccgag accagcctca
     1201 ttcgccatca ctgccggggt gctcgtgacg gcaccgggtc tcctggcggc gacgacggcg
     1261 cagggagacg ccctcgccgt ggcgatgcag ctcggcgccg ttgccatcgt cgctcctcgc
     1321 cctcgcgatc cggcagccac gatcgcggcc ggggcactct gctcgttcgc cgtgctttcc
     1381 aaggtcagtg cgatctgggc gccggttgcc ctcggcgcgt ggctgctcgc ggaacgaaga
     1441 cgcctgacgc tcctgctctt cgtcgtgagt ttcgtgctgc tgctctccgc gcaggtgctc
     1501 atcctccaga ccctcaccga gggaaggatg ctcgagaacc tccgtacgac gctcctcgcg
     1561 ggggtcactg ggccagctgg aatcctcggg tccccgcttc gcttggtgaa cctgctcggc
     1621 ggtattcctc tcctttgggc cgtgctgccg gtcgcagtca tcggtggagc gatctgggga
     1681 tcggaagaca gtcgcccctt ctacctgagc ttgctcaccg cgttcctcgt cgtcctcgtt
     1741 gtgctggccg acgaaggcac cgtgcacaac caccttctcg acgtcacggt gttgtctctc
     1801 ctgttcatcg ggatcctctt cgggagaacc accgaggtgt ctccagctgt cgcactcgta
     1861 ctcgggtcgc ttcttctgtg gtccatcggc acgtcatacc tcctggagct ctggccagcg
     1921 acgcgggagg ccgggatcga agtggttcga gggatccgct cgagcaggta cgacccccgg
     1981 ccccttggtc gcgtcatccg tcccggagag tccttactct ccgaggatcc gtatgtcgcc
     2041 gtggctttgg atcggccgcc tgtcgtactc gacgcttgga cgttgctacg gctcgggcgc
     2101 gcgtcttccg taccggcgga ttccctggcg cggcggatcg aggcgaggga gttcgactgg
     2161 atcgtcctgg tgcgcccgat cgaagacgaa gagtggtact cggacgtcca cttcggaacg
     2221 atcgtggccg atgccatcag cgggagctac cggatccagc ggcaggcctc cggctaccac
     2281 gtttacgtgc cccggcggtg atgagcccgc tccctggtgg tcgatcagct cgcttcggct
     2341 ggggcataga tgtgatacgg acctctggag agctgatagt cgtacgagtc cctgatctcg
     2401 gcggcgatct ccggcccgag atggaaatcc tcccaccacc aggcgatgtc acgattctcg
     2461 acccgcctga gcagcacaac cagctcgaac tccttcgcgt cgatccgtcg gaggagctcc
     2521 gcctggatct ctcggtccac ctcgacgatc cgctcgacca tgaaggggtc gaggagcacc
     2581 ggtcgttgcc cgagtgacac cggcacgtac ggatcctcgg agaggatggg cgtctcggag
     2641 gaagccagcc cgcggagcgg ctgcggatct aactgagggt ccgtggtggg atcggcgagg
     2701 cgaaccacgt ggcgagcctc gggcaggacc acagtgacga ggccggtcac tcccacccac
     2761 aggaccatga gcacgaccgc cgtccccgcg accctctcat aaggaaggct ggggagcgtc
     2821 cgacccacca gacccccgac cacgatgagg acgagaaccg caatgtcgat gaggtggttc
     2881 caatccgccc cgacgtcggc gaggacgacc acgaggatcg cggaggcggc gagcagcgcc
     2941 aggagccatt cctcgtcacg tcgcccccga aacgcatacc agcccgccac agccgcggcc
     3001 ggcatcagaa accagatcgg ggtcgcgtgg tcgatcgcca ggagaaccaa ccggtagggt
     3061 gcccggagca gtccgccttc cgcgagcccc gcccccgcca gcccgagtac gttggagagc
     3121 atccgccccc cgctgagggc cgaaaacaga ccgaggaggc ctaccgccca aacgacatac
     3181 cctcccacga accaactcac cgaccgccga ttgcggcgaa acagccacag cacgaccgcg
     3241 atcggagccc agacggcgct gagcttcgcg gccagcgcga gggcggcgag aagcgcggcc
     3301 acgatcatgg ccacgggcgt cgcagagcgg tgtggtcgac tcgtcgccaa ccacaccgag
     3361 gacacttgga gcagcaccgg gagggcatca gccctgaggc tcatcgtcag cccgaatccg
     3421 gcctgtgtga gcagcaacaa cgaggccagc gccgcagcca acggccaggg gcaaccgagt
     3481 ctccggatga cgaggaaggt caccaagatg agggtggcca tcgtcatgta agcgaggacc
     3541 ttgccggaga cgagatactc gccggtgaca cgcgccgccc cggcgtggag gagcaccgga
     3601 aggggcatcc atcgggtccc gccgtagaag ccgtcgtcgc tgagctgcgg atagagcgtg
     3661 ccctcgttca cgtaccacgc catcgcgatc cgagagcccc ccacatggtc gacgcggtaa
     3721 cggtcgtccg cgtgcaccca tgccacgacg atggtcgctg tcaacgccaa gaccgtcagc
     3781 cccacgagcg ccgcagcaag cacccgctcc gcagggggac ctccggccac gggcatggga
     3841 cgattcccta cctgctcggg ttcggccgcc atgggcacac gagtgtagag gccggagatc
     3901 ggctccacct aggatgaccg gttccccgta ccatgaagcg ccatgttacg ccgctcgtgg
     3961 tggctgctct tcgtggtgtt cgccgtgacc cgggtcggac tgatctacgt gggcgtggat
     4021 ccctcgatct atggaggccg cgaccacgac atcgggagcg acgtgtcgat ctacgccgga
     4081 tgggcggacg ggatcatcga gggcgatgtc ccctaccggg atgagccgat cgagtacccg
     4141 cccgggtcgc ttcccttcat cgtggcgcct gcgctgggga aggccatcac cggactcccg
     4201 taccgcgcgt tcttcggtgc ggcgatgctc gcggtcgacg tcctcggact catcgccgtc
     4261 ctccgtctgg tggggcacca ggtgtcgaga gccgggccgt ggctctgggt gctcggtctg
     4321 gcggcgctcg gaccggtcgt ctggaccagg ctcgacctgg tcccggccgt cgccacgctc
     4381 tgggccctcg agcggatcgt cgcccgcgcc tggacctcgg gaggagtcct gctcgcgttc
     4441 ggcgcggtgg ccaaggtgtt cccggggttc ctgttccttc ctgcggcggc ggccgcccca
     4501 cggcggacgc cgttcctggg ggcaggggcc gccacggccc tcctacttct ggcgccgttc
     4561 ggggtggtgc tgcacccgat gctctcctcc gtcgcgggct atcaccttgg ccgggggatc
     4621 cagctcgaga gcgtatgggg tggagcgatg ctcctgaccg gcctggccgg ggctccggtc
     4681 tccgtcgaga agcgcttcgg cgcctgggag acgctgggcc cgggcaccga agccctcggc
     4741 atcgcctcca cggtcctcgc cgtcggggtg ctcgcgggat cggtagcggt ggcgtggcgg
     4801 cggcgcgacg agcctctgcg gctcgccggc gtctggttcg ccacgcttgc cctgctcctc
     4861 gcggtggggc cggtcctctc accccagttc atggtgtggc tcgtggcggt cggcgctggc
     4921 gcgctggcgg tcgggtccga taccgtccgg aaggccgcgc tgttcctcct gcccctcgcc
     4981 gtcctgaccc atctcgtcta ccccatctcc tacgacgggt tggtcgccat ggaacccgtc
     5041 agcgtctggc tgctcaccgc gaggaatgcg atgctggccg ccctggcggt gtggtcgacg
     5101 gtgggcgtcg tccgcgatcg gccgactgag acgcatcgcg cggaccgtgg atccaggcgg
     5161 ggctgggagg caccatgagc gagccaagac gccaccgggc ggcaccccgc cgcgggagtc
     5221 ctcgccacgg cgcttcgctg gtgggtcggt cgctccggtg gctctcctat ccgcacggga
     5281 aagcggacga tcgcaacgtc gcgcttagta gggtcttgga acgcgggagg tcgcggcctt
     5341 agcttcttcc cagacagatc gccggcggtg gtgggaggcg gctcgacccc ttcccccgat
     5401 cgtctccgac gggctcttcc tcgtcgggct ggtcgttgtc tcagccctcc cctacatcgg
     5461 ccgtctcggc ttctactcgg acgactgggc attcctggcg gtgttatcca ccgccgagga
     5521 tcaatcgctc acgagcctcg tccgtgacca gtacgcgacg aacccgaacc tggcgatgcg
     5581 gcccacgcag gtcgtctacc aggccttcct gttccgactg ttcggcctcg agccgctcgg
     5641 ctaccacctc gtcaacctcg gcgtcctcat cgcggtagca gtgctcctgt ccctggtact
     5701 ccgtgagctc cgcctgcctc ggggcctcgc ggtggccgtg ccggccgtct acgcgctcat
     5761 gcccaactac gctacggccc gattctggtt cgcctcgttt ggctacctgc tggggatggc
     5821 tctctacctg ctggcgttgt acgccgacct cagggcggcc tcgagtgaac atcggaggac
     5881 cgtgtggacg tggaaggccc tggccctggt gacgctcacc accgcggcgt tcgggtacga
     5941 ggtggtgata ccgctgttct tgctcaacat cggcctcgcg gaggtcgtgg ctcgacgact
     6001 gccgtcccgg gggttccggg cacggatggg gccggcaggg acggccctgc tgcacggcag
     6061 cacgctggcc gtcgccgtcg cggccggcct ctacaaggcc tccgtcgccg tgggcgtggg
     6121 ggtgggatcc tcaccccttc gttacacgct gtggctcggg tcgagctcgg tcgccacgaa
     6181 cttcggcacc cacggcgttg ggctcccgca caccgtgggg tgggccctcc cgcatgctgg
     6241 gatcggcggc gtggcgatcg cgatcctggt cgggctcggg atctaccgct accttgccgt
     6301 cgttcgagac cctagaagtg agggcgacgc gctgccgttc cgcggaggat ggctccggat
     6361 gggcgtggtg ggcctcgtgg tgttcgccct cgggtactcg atcttcctca ccaccggcag
     6421 gatcgcgttc tcgagcaccg ggatcgcgaa ccgggtggcc accgcagggg ccctcggcgc
     6481 ggcgatcgtg ctcgtcggag cggtggggtg gatcgcgagc cggctgcggt ccccgaggct
     6541 ccgggatcgc gccttccgtg cggggatcgc cgccctctgt ctctcgggga tcgtcgtggt
     6601 gaacggactg gcctccttct gggtccagag ctgggaggaa cagcgcgagg tcctggcgga
     6661 tctggacgcc gctttcccca gactcgcccc tggctccacc gtgctcctcg gcggggtctg
     6721 tccctacgtt ggcccggcca tcgtgttcga gtcgagctgg gacctccagg gggcactgcg
     6781 ggtccggcac ggggacaaat cggtcgacgg cgacgtcacg agcggcgtcg tctcggcgga
     6841 gccgcagggc gtcgtgaccc agatctacca ggtcgaggtg atgcacccgt acggcgctcg
     6901 gctgttcctg tacgaccgtc gagcgggaac cacgacggtg ctcgtggatc agtccgcagc
     6961 ccgacgagcg ttgtcgacgc aggtgtcggg gaccgactgc cccgagggtc gacccggcgc
     7021 cgggaccatc gacctcccct tcgacgtctt gttccattgg ttccggagcc gcattgggct
     7081 gtgagcggtt cgctgggata ctgctccaca tcgtgacgga cgaccagggc tcagaccgcg
     7141 gggacgatcg acccgccgga gcgtccaaac gcccgaccgt cgggatgctg cactggcccg
     7201 atcggttcga agacttccac gaccggctgg gcgtcacgtt cgagtccttc cgggacgagt
     7261 tcaccggcag ctgggtgttc aactacgtgg aggctctcca gcgggccggg gtccgcccgg
     7321 tcctctacta cgcgtccgcg cggatccggg agccgcttcg cttcacccac cgcccgaccg
     7381 gggcggcggt gcgggtcctc ccctcgcccc gcctgcacca gaagctccgc ggcgcgcagg
     7441 agcggttcgg gatccgatcg gcgtccctgc gagcgctagg gtcctacctc gccacccccc
     7501 tgcgcccgct ggtgcgggcg ctccgcgagg acggctgcgg cgcgatcctg tgccaggagt
     7561 acgagcaccc ccggttcgac gtggccgtgg ggctcggacg actgctgcgc ctccccgtgt
     7621 tcgccacgtt ccagggcggc gaggcccccg cgacgaggct cgaggtcccg ctgcggcggc
     7681 tggcggtccg gcgggccgcg ggcctgatcg tggcctcggc ggcacaggcc gagcgagccc
     7741 gccggcggta cgggctcccc tcggaccgga tcgcgaggat ccccaacccg atcgacgtgg
     7801 accggtggcg tccgatcccg cagacggccg gacgggcgga gctcggcatc ccggcggacg
     7861 cgccggtggt cacctggcag ggacgggtgg cgatcgacca gaaggggctc gacgtgctgg
     7921 tggaggcctg ggcccgggtc tgcgaatcgc acggggagga cgcccccctg ctcctgctgg
     7981 tgggacacgg gcaggacgcc gagacgctcc gagcgatgct ggccggcctc cccccacacg
     8041 ccgtccgctg ggtcgaccgg ttcgtccacg accgggaggc gctgtggcgg taccgggccg
     8101 cggccgacgt ggcggtcctc ccctcgaggc gggaggggtt cccggtggcc gcggtggagg
     8161 ccatggcctg cggtctcccc gtcgtggcgg ccgacgcgct cggcgtgcgc gatgccctcg
     8221 gggagggcga ggaggccgcc ggcgtcgtcg tcccccggga ggatcccggc gcgctggccg
     8281 ccgcgctgga gcgggtcctg gccgaacccg acctccgccg gacgctggga gagcgcgggc
     8341 ggcgccgggc ggaggagcgg ttctcgctcg acgtggtggg cgcccggctc cgcgacttcc
     8401 tgttcgggcg gcgcgcgtga cggaccgggt cgtccggacc ttcccctaga ctctacgcgt
     8461 gatgggtacg gggacgcggg aggagtggat cgagaaccgg ccgagccgcg gcctgcggct
     8521 gccggacctc cgggagctgt ggcagtaccg cgagctcgcc tacttcctgg ccctccgaga
     8581 cctcaaggtc cggtacaagc aggcggccct gggcgcgggg tgggcgatct tccagccggt
     8641 ggtgctgacg gtggtgttca cgctggtgtt cacgaggttc gcccgggtcg agaccgccat
     8701 cccgtacccg atcttcgccc tctccgggct gatcgcctgg acctacgtgg ccggcagcct
     8761 cacccgatcg acccagatcc tcgtggcgaa cacccccctg gtgaccaagg tgtacttccc
     8821 gcgcctgctg gccccggtgg cctccgcgct cccggggatg gtcgacctcc tgctttccct
     8881 gctggtgctg gcggtagtca tgcccttcta cgacgttcgg ccgacctggg cgatcctcac
     8941 cctgcccctg tggctggcgg cgatgctcgt cgtggtgctc ggggtcagcc ttctgctcgg
     9001 caccctcaac gtccggtacc gggacgtgac ccacgggatc acgctcctgg tccagctgtg
     9061 gatcttcgtg agccccgtgg cgtacccgag cgacgccgtg ccggaggcct ggcgggtcct
     9121 ctacttcctc aaccccgtgg ccggcgtgct cgaggggttc cgctgggcgc tggcgggggc
     9181 ggcgtggccc ggctcggtcg tcttcctctc gctcggcacc gccctggtcc tgctggccgc
     9241 gggcgtcctc tacttccagc acgcggagcg tcgcttcgcc gacgtgatct gagatgcacg
     9301 cgatcgagat cgaggggctg agcaagcggt acttgctcgg cgaggaccgt ccggggggga
     9361 ccctggtcga ggcgatgagc cggtcggccc ggcgtctgct gcggatgcgg tcccgtccca
     9421 ggcgggacga ggtgtgggcc ctccgggacg tcaccctggc ggtgccagag ggggaggccc
     9481 tcggggtgat cggccgcaac ggggcgggga agagcaccct gctcaagatc ctgagccgca
     9541 tcaccgagcc gaccgccggc gtggctcgga ccagggggag ggtgacgtcc ctcctcgagg
     9601 tggggaccgg gttccacccc gagctcaccg ggcgggagaa cgtgttcctc aacggggcca
     9661 tcctcgggat gcggcggcgc gagatcgagc ggcggttcga cgagatcgtg gccttcgccg
     9721 gggtggagcg gttcatcgac acgccggtga agcggtactc ctcggggatg tatctccgct
     9781 tggccttctc cgtggccgcc cacttggagc cggacatcat ggtggtggac gaggtcctgg
     9841 cggtcgggga cgcggccttc caggcgaagt gcctggggcg gatggagacg gccgagcgcg
     9901 aggggcgaac ggtggtgttc gtgagccaca acctggaggc catcgccaag ctgtgccccc
     9961 ggacgatctg gctggacggc ggagagatcc gggaggaggg ggactccccg gccgtgatcg
    10021 acgcgtacct ctccgaggag gtcacgcggg cctcggagga gaccgtggcc ggggaccccg
    10081 gggccgccgt ggtcctccgc tccgtgcggg tcctgggaga ggccggtgag ccgctcgaga
    10141 cgctcctgcg cgacgagccg ttccacatcg agctgcggtt ctccgtccgg acccaggtgc
    10201 cgggcctcga cgtcgccgtc tgggtggcga acgcgttcgg gacggaggtg ttccgcgagg
    10261 gatggttcga tcactcgccg gaccgtccga gcgagccggg cgaatatgtg gccacccttc
    10321 gggtcccgcc cgtcctcaac gtcggggagt acaccgtcgg ggtgtggatc ggctcgccgt
    10381 acgagacgct ggtggacgcg gttgtggccc ggttccacct ccagggtgac gtgaagaacc
    10441 gcccgtcccg gtcggtggtc ctgcacctgc cgtgggaggt gcggaggatc agcggcgccg
    10501 acgacccgac ccctgccccc caccccagac gatgaaggtc ctgctgtcgg cgctagcctg
    10561 cgagccgggg aagggatccg agcccgaggt ggggttccgg gcgatgctcg cggcggccag
    10621 ggagcacgag gtctgggtcc tgacggcgtc gcagtcgatc ccggcgctcg agcgggccct
    10681 ccggtcggat ccgcgcgcag agcggatcca tctcgagggg atcccgttcg gcgtcgacgc
    10741 cgagacgatc gtccacttga acgccgtcgg ctaccaccgt cggtacgagg agtggcagcg
    10801 gctggcagcc gaccgatctc tcgagctcga agcgagggtg ggattcgacg tggttcacca
    10861 cgtcaccctg gccagctact ggacaagagc cggcgtcgcc gcggtgcgga agccgctggt
    10921 gtgggggccc atcgggggcg gcgtggatcc cccccttcgg cttgtgccgg aactcgggct
    10981 tcgggggatg gtggaggccg ccgcccgagt cgtggggaga cctctgatcg cccgacttcc
    11041 gcctatccgg aggacccagc gggttgcctc gctgatcctg gcccagaacc ccgggacagg
    11101 acggagactc cgaagcaggg ggacggtgaa gctgttgtcg aacgctctcg ccgtcgagct
    11161 ggacggcgtc ccgatcccga agaagcgaac cacggacctc ttgttcgtgg gccgcctcct
    11221 tccctggaag gcgcccatct tggctcttcg tgccctccgg cacctccggc atccccgtgc
    11281 tgtgctgcgg ttccttggag acggtccaga gcagtcgcgc ctggagcaga cggccaggaa
    11341 gtgggggctg tcggagcgcg tccagtttga gggctggctt ccccgcgacg acgtcctcag
    11401 cgccatggct tcggcaggag ccctcctcca tccggcggtg cacgaggaag ctgggctctg
    11461 catcgcagag gctctcaccc tcggtacccc ggtcgtctgc ctggaccacg gcggacccgc
    11521 cgagatcgtc gggcagtggc gcggaagacc ttcggccctg gtaaccgcga ggggagccga
    11581 ggccaccgcc cggagcatcg ctgccgccgt cgatcgcttc ctcctgcatc cgcctgagcc
    11641 ggcgagcggc cccgtgacgg ccggtacgtc gttccaggac gagctcctgt ggagctatga
    11701 acgagtgacc gccgcgtccc cggggtaggg cacccgagac cgctaaaggc accgagtcgg
    11761 agccccgagt gaagggagta tgtctgtggg acaatccgct catgaaccga ctcggtcccg
    11821 gcaccctgac ggcatgagcg gggacaccgc ccccctggag cttcgggact acctcgccct
    11881 cctgtggcgc cgcaagtgga tcatcgccgc catgaccatc gtcgcggtgg gagccgccct
    11941 cttctactca taccgccaga tacccgtgta tacgtcgtcg gccgaggtgg tggtccgtcc
    12001 catccaactg gagccggctc agtccgcccg aggcacaacg gggttcgtga acatggagac
    12061 ggagacccgg gtcgcgaatt ccgggcccgt cgccgaacgc gcccttgatg agttggagga
    12121 ttccggtatc gagccggggg acgtctcagc cagccagatc gaggagtcgg agacgctcct
    12181 gttcaccgcc accggcctct cgccggaaag cgcccaggcc tcggcgcaga actgggccga
    12241 ggcatacctg gggctccgga aggagcaggc tctcgccgac cttgaagagg tccgccgacc
    12301 gctcgaggat cagatcaccg tcctcgggca acaagcggcg gtccagcgac gcatcatcca
    12361 ggagaaccag gagtccaaca tcgccgtcgc caccaccgcg cagcagcggt acgcggcgat
    12421 cctggcggag gtgtccctac tgcgacagga cctgaaccgg tacatctccg ccgggagcct
    12481 acacgtggga gaggtcctcc gtgcggcgcc gacccccacc gctcccgcca atcggaacca
    12541 cgtgacgacc gggggcctcg ccttgctggt ggggatgtct ctcggcgtgg gggtggccta
    12601 cctgcgggac cggctagacc cgcgggtacg ggggacgcga gagctggagg ttcacagcgg
    12661 aggctcggtc ctcgcgttga tcccctccgg caagccactg aaggacaact tgcccatcac
    12721 catggccgag ccggcctccg agctggccga cgcctacatc gcccttggct tccgcctcct
    12781 ccgcatggcg ggggaccggg agatcaggac actggtggtg acgagctccc tcccccagga
    12841 ggggaagtcc tccaccgtgg ccaacctggg cgtggccatg gcccgatccg ggaagcgcat
    12901 catcttggtc tccgccgacc tccgaaagcc cggcctcctg acctacttcc cccaatggaa
    12961 cggacgaggc ctcgtggaca tggtgcggga tgggaccggc atgagcgagg cgctctccac
    13021 cacatcggtc gacaacctgt gggtgctgca cggggggtcc tctctgcgag gatccagccc
    13081 gctggagatc gtctcgtcgg acgagatgcg gcgcctgctg atcgagctcg gcagctgggc
    13141 agacctcgtc ctgatcgaca cccccccact cctcggcgcg ttcgatgcgt ccgcactggc
    13201 cgccttcgcc gacggcgtcc tgttcgtggc cgacgggaag cgcgccagcg gcaccacggt
    13261 cgaacaggcc cggcgcgagc tggagctgag cggggcgaac gtcgttgggg tcgccgtgaa
    13321 ccgatacgac cctcgcgact acgcgggcta cgcctacagg gccccgtact accgccccga
    13381 gaacggcagg gtcctgccgc cccccgagct tgagcagccc caggtctcgc gggaagagcg
    13441 ctagcaggtc ccggtcgctc tcggagcgcc tcactcacct ccggcgtcgg gccccggcgc
    13501 cgtcagcctg agctcgcggg ggcccgcggg acacgatgcc cgcggttccc caaccccgcc
    13561 accaggcccg gcaggaggcc ggtcacctcc ccgaccccca tggcgaacag ctctcggttg
    13621 ccggtcgcca cgccacggag gaggagccac ggagcccgga ccaggtcgcg gaggacccgc
    13681 cgggcgatga acaggtcccc ccggcgaagg tgcttcccat agaagctccc ctgcccgcgc
    13741 gcgtagcgac ggtacagggc cgtgcgctgc gagggatcct gccaggcgag gtgacccacc
    13801 acgatcccgg gctcgtagat gatcggcacc cccgcccgga gggcccggta agcccagtcg
    13861 ttgtcctccc ccgccacgtt cagggaggga tgctcgtcca ggtacccgat ccgctccagg
    13921 agccggatcg ggaaccccat gttggctggg aacaggacgt cgccgtcgat cagcggctcc
    13981 ttgtacacgg ccggctccgc ccgggtgatc accgttagca cgagcccctc cccctccggc
    14041 tccacgcgcc cggtcacaat ggcgtccccc acgttacgaa cccgggcggc gagctgcccg
    14101 atccagtcct cccgcgcccg gcagtcgtcg tgggtgatgg ccgtgtacgg cgtgcgaacc
    14161 cgctccagcc cccgattcgt ggcggcggcg atcccatgct ggttggagcg cagatggacc
    14221 acctccagac cgtcggccgt gagggcgtcc acccacctct cgacctccgg gttctgggac
    14281 tggtccacca cgatgagctc cgccggccag gtcgcaccag accggatcga ctcgaggcag
    14341 ccgcggagga ggtcgcgccc cagggtgggg atcaccaccg aaacatcctc cgccacggcc
    14401 cgccccgcgc tcacgtggcc tcctccagca gctccgggaa cgacacgacc aagtccacct
    14461 cgtcgggccc cgggcacggc cggtcccaca gctccccggc caagaggaac gtggcccgac
    14521 gtcccgtgtg gtactccagg gcatcgcggg ccgcccactc gtagatcagg tcgtggacgg
    14581 cgtacgcgcc gcgaggcgcc cactcgacca cgctacggcg gaccccgcag acggccacca
    14641 cgtccccgtc ctcggtctcg gcggcgatcc tcgccgagga ggcctcgacg acgtctgcgg
    14701 agcgcagacg cacgctcacg ctcagggcca gggagagcac cgccccggcc gccaggaccc
    14761 cggccaccgc ccccacggcg gcgcgtcgac ggaagccaac gcgggcccca atcaccgcga
    14821 cgagggccga cagcaccagc cacgtcgggg tgaacagcct cggcgacccc tggcggggga
    14881 ccaggagcac caccggcacg ttgaccagca cgatcagccc cgcaccggcc aggatcgccc
    14941 gccggatgaa cccaccgttc cagaccgtcg cggtccccgc gaggatcagc gggcccgcgc
    15001 gggcgccgag gagccccccg acggccagga cggccaggct caccgggaag gcccaggcca
    15061 ccgccagcgg tacggagtgg agccccaacc ccaccgcggg gaactccagg tagaaggaag
    15121 gatcgcggaa caggctggcc acccgctccc ccagcgacac accgagccgc ggatcctccc
    15181 cgggccacag gacgaaggcg gcgaggacca tcacgaccgg cacggcggtg gagaccagcg
    15241 ccgcccgacg ccgatcccgc gcggccacga gccaggcagc cagcgggagg gccaggaggg
    15301 actgctccac cgagagaccg gcgccgagta ccgcgagggc cgcccacccg tgccggccgg
    15361 cgcggtggag ccgaagggcc cagagcgcca gcgccagtcc cagggggatg tgcagggcgg
    15421 caggccacag cgcggcctcg gtccccaatg gctggagcag ccacacggcc gcgccgactt
    15481 ccggccaggg ggcaggcgtc cccaggtccc ggaggaggcc ccgcagcagc agagccacgg
    15541 cgagggtgag cgccagaggg acggcgatgg ccaccccgaa cgggatcgtc tggcacacgc
    15601 cggtggtgac gaggatctcc aggaagtggg cgggacgcat cgcccccagc cgctccaccg
    15661 agtcgccgag cgcggaggcc agccccaccc gctgcggctc catgcacttg gagaagtcgt
    15721 cggccagcgg cgccccccgg agcaccacgg ggatcgacac ggccacgaag acgacggcca
    15781 ccagcagcag ccccagccgg gctccacggc cctccggcgc ggcctccggc accccttcct
    15841 ggcggcgcac cttcacggcc cgctgcctct gccactcgcc agcggacgga cccactcctc
    15901 cgcccccgcc gtccacgacg gggagccccc cctcgcccgg gcccgccgct ccgcgacgcc
    15961 aggccacgcc agggccacga cggcccggac gatcgagccc gggtcccccc ggagggcggc
    16021 cgcccagaag tatcgggccg cccgggcgcg tcgccgatcc cgaagcgcct cccacgcggc
    16081 ccaccggtag tggcgcacca ggtccaccgc gatgccgtgg cggcgtgcca ccacgtcgag
    16141 ctcctccagc atccggggca tggcactcga tctcccgcgg cggacggaca ccgccaccag
    16201 cggctccgga acgcaggccg gcgggccggc ccgggcgaga cggatccaca ggtcccagtc
    16261 ctcgctggtg gttaggtcgg ggtcgaatcc ccccacccgc cggagcagat ccgcggccac
    16321 caggacgctc gacgtccccc caggaacggc gtcgtgccgc gcgagagccg ccaccacctc
    16381 ctcgggcgga ggaggcgggg tcccgtcgac cacgcggagg cgctcgtcca cgatcacctc
    16441 gccaccgtag acccaccgcc tgccggtcgc cgccgccgcg tcgagctgat ggcggagctt
    16501 ctctggggcc cacaggtcgt cgtcgtcgag gaacgccacc caagcccccc gagccatctc
    16561 gatcccgcgg ttgcgcgccg cgctcacccc ggcgggggcc tcgttccgaa ccgcccggac
    16621 ccgcgggtcc gcgatgcctc cgaggaggtc gtgggtgccg tcggtggagg cgtcgtcgac
    16681 cacgatcgcc tccagatcga ccgcccgctg acccagcacg ctccgcagcg ccagcgacag
    16741 gggcccggcc cggtcccggg tggggatgat cgcgctgacc tcggggccgg tcaccggatc
    16801 cggtcggccc ggctcaacct gatgctcaga cgcaccagga gctcgtcggg cggcaggcgg
    16861 ccgggctccc accgccccag caggtggcga tcctccctcg gatgcatcgg ccgcggccgg
    16921 cccgtccatg cagccgcgta cccggcctcc cggaccttcc tcgcgacccg cccgtctgcc
    16981 ttcccgtgcg ggtaggcgaa caggtccagg gggcggccga ccacctcggc cagctccccc
    17041 cggccagccc ggacggcctc ctcgaggtcc tggtcgggaa gccggggcag caccgcgtgg
    17101 tcgacggtgt ggaacccgat cgtcatcccc gcgtcggcga gggcagcgat gtcgcctggc
    17161 tgcaggtggc ggacctcgtc ccgtccctcc tcctcggcga cccgccggag gcgctcgtcc
    17221 gcctcgcagg caaggatcac cgcctccagg tcggcgggca cctccagacg gacggccacc
    17281 tcggagaccc ctcgctcccg cacccaccgc tccagggctt cgaaccagta ggggccggcc
    17341 ccgtggagcg atcgcccgct gaggaagaac gtggccggca gtccgagctc gcggaggatc
    17401 ggcagggcag acacgacgtg cgacgggtaa tcatcgtcga aggtcagcgc gaacctgggc
    17461 gtcccccggt cggcgccggc cagcaggtcg gcgaggggca ccacccgccc gacctcggcc
    17521 agagcctcca cctgccggcg gaacagctcc gccgggatcg aggggatcac ctgcggcccg
    17581 gagatctccc cttccgtcag ccgatggtag aggagcacca ggctacgacc ccggagcccc
    17641 gcgatccggc gggacagggc tctgaccccc cggatcctca gcgcggcccg ggccagccgg
    17701 cgcgccgcat cgctgattga cgtagggagt ctcatccccg gccccgctga agggcctccc
    17761 gggccaggtc gtccagccac cgctgggcct cccgccgcca ctcctcatcc tccggacgac
    17821 tacggatcag ccggaacacc ctgtcggagc ccgcggcggg gtggagcagg gccacggccg
    17881 cccggcccac cgaccggagg tcgcccatcg agacggccat ggcgtagtgc cccgccgccc
    17941 tgccccgccg gcccgcccgg agccacgccc aggccgcccg gcgctgcatg gcagctcggt
    18001 cgacggggat cccgtatcgc tcggccagca ggtcgggctc gcggacgatc gacgcagtct
    18061 ccagcggcac gttcgaggga tggaacctgt atgcgaccag cggccggggc acccacgccg
    18121 gcggccccac ccgcgccaga cggatccaca tgtcccagtc ttccgtgcgc ttgagcgtgg
    18181 tgtcgaatgc cccgacgtgg acgagcgcct ccatgctgac caccacgttc gacgcccccg
    18241 tgggcacggg gttgtatctc gggagggcgg ccaccacctc ctccggatcg gggggcaggc
    18301 tgcccgtcag gacccgcagg tccgcgtcca cgttgacgtc ccccccgtac acccacgccc
    18361 gccccgagac cctggcggcc tggagctgcc ggaccagctt gtcgggcgcc cagaggtcgt
    18421 cgtcgtcgag gaaggcgacc caagcgccgc gggcctcgtt gatgccccga ttccgggcgg
    18481 cgctcactcc catggagcgc tcttctcgga ggagccgcac ccgcgggtcg tccaggcccg
    18541 gaaccgatgg cgcggaagcg gacccatcgt cgaccacgat gacctcgacc tccacgtcca
    18601 gctgccgcag ggcgctcgcc agggtaagtc tcaggacgtc gccgcggtcg cgggtgggga
    18661 tcaccacgga cacgtccggc gtcctcgagg ccaccacggc gtcagcgctc cttcaccgcc
    18721 accatcacca cgttcttccc caggggcggg tgccggagga aacgctggat ccaccggctc
    18781 aggggcacca tcagccggtc gtacccccac acggtggagc tcccgatgct ggtccggtgg
    18841 agccacctgt agaccacgag gtacgggagg accccgacca cgtcgaagta gtagagcgac
    18901 accacccgca ggccggcggc gtcggcgagc cgccggagcc gccccaacga gtagcggcgg
    18961 tagtgcccgg ccttgtggtc gaggaggctg tacaggcgct ccagggccgg cccgaacacc
    19021 agcagcgcgc cgcccggccg gagggcctcg gcggccatcc gcacctcctt ctcgtcctcc
    19081 tcgatgtgct caagaacgtt gacgtacagc gccgcgtcga aggattcgaa ggcccggcgc
    19141 ccgtagtcaa ccagggtctc cctgcgggcc tccacccgcc gggtaagggc ggcgtacggc
    19201 tcgagctggg cgaacatgtt ctcggcgggc tccagggcca cgatcgacat gtccggaaat
    19261 cgctccacca gcttccgagt gatcgtcccg gcgccggccc ccacctccag gacccggccc
    19321 cgaaggtacg ggccaaagtc gtccagcacc cagtcgaaga agtgcttgct ggccgccagg
    19381 cattcgaggt cctcctccgc ggcgaagtgt tcgaccggga aagcgcgccc cgcgggggac
    19441 aggcggcgcc ggcggaaccg cagcacgtcc gtccggagag ccaccagatg ggaaacgaac
    19501 tcccccaagt ccctcatccg gagcccgccc ccgccggcga tcggccgccg gtagatgatc
    19561 gaggcttccc ccacccggta gcccttggcg atggccgtgg ccacgaaggc ggtgtggagg
    19621 ctgtagctgt tcaccggtat ctccccgaac cggaactctc gcaccacgtc cgtgcgggcc
    19681 acccgaaagc tggtggtggc gtcggccaca cgaggcgtgg acgtgatcag gcggaacgcc
    19741 gcgttggccg ccttgccaag cgcccaccgg gagaaggcca tcccaggggt accgctccca
    19801 ggggcccatc gcgacccgat caccacgtcg agacggcgat cgacgaggac gtccacgagg
    19861 ttggggatct ggctgggatc gtgccggccg ttggcatcga gggtgaccac caggtcgctc
    19921 ctcccctcct cgaccacccg ccggaacccc tccaggtagg cgagcgcggg accggccggc
    19981 ggtgccggga aggtgatcag ggtgatcccc agctcctcgg ccacctcagt ggccgtggcc
    20041 ccgcaggcgc cgtcgtcgaa caggagaacg tcgaggtcga tcccccgggc ccggagggcg
    20101 tacgccgcca cggcgacctc gcggaggacc ggaccgatcg ccggcccgac gtcgtaggcg
    20161 ggcaggatga tggtggccgc ttccactcgg ttcataccgt atctgcctcg agccaggatc
    20221 ggcctcggga gcaccgacga ggtgcctcga tccggccgct ccagggtagc cgctctcggc
    20281 gagcttcagc atcaccatcg ggcatctgcg cgtactgtag ggcgatgcgg cttgctcacg
    20341 tggtcccgcg ggaggaacaa ccgtggtccg ggatcctcac cgtgatcgtg cacctcaccg
    20401 cggccctgcg ccggcagggc cacgaggtcg aggtgtggca gctccaccgc tggaacgatc
    20461 gctacgcggc gcagcgccgc gtccttgagg ccgccgaggt ggtggagatc cccgtggcgg
    20521 tggacgcccc cgcgtggcgg gtgggtcgga tcgccgcctc gctggccgag aagcggagca
    20581 tcgacgtcgt ccacctccac ggggcgttca acatctggac cgcgctcgtc ggccgggccc
    20641 tccgccgtcc ctacgtcttc tccccgcaca gcggctacga tcccgtgtcg ctgcgacgga
    20701 gccggttccg caagacggtc tacgggctcg ccttcgagcg gaccctcctc cgccgggccg
    20761 cgctcctcgt gggcctcacc gaagtcgagg tggcccagct ccgggctcgg ggggctcgcg
    20821 gccccgccgt ggtcatcccc aacggggtgg ccccgccccc ggagggggtg gaccctgcgg
    20881 ccttccgaga ggaattgggc atctcgcccc acgcgccttt ggccctgttc gtggggcgcc
    20941 tcgacgtcca ccggaagggg ctggacgtcc tggtcgaagg gatcgccgac gctccgggat
    21001 ggcacctggc cctggtcggt cctcggttcc gggacgtgga gcgcctggag gccgcgatcc
    21061 gccatccgga cctgagagac cgcgtccacc tcgtcggagt caggtacggg gacgctctga
    21121 gccaggccct ggcaggggcc acagtcttcg ccctcctgtc ccggtgggag ggcctgccga
    21181 tggccctgct ggaagccctg tcgctcggga ccccagccgt agtcagcccg gccgtggagg
    21241 gcttggtccc ggtggccgcg tcgggagcgg gctgggtcgc ggacgacggg cgccttgtgg
    21301 acgtcctccg cgaggcggcg tcgggcgccg cggcccgcgc cgaggcggcc cgagcactgg
    21361 ccagccggta cgactgggat gccgtggccc gcgagtacgg tgccgcctac cggcgggccc
    21421 gcgaccgggt cgctacggcg taacctgcgc cggccggtac aggtggtacc cgccgaaggt
    21481 ccggtcccag cggtaggcct cccgcatcgc cgctaccaca cgggtcccga agtggtagtc
    21541 gcgccaccat ccgtcgttcc cctccagggt ctccacgagc acgagggcgt cgaactcctg
    21601 ctctcggatc cggtccacca ggtcgtcgac gagttctggc tcccgccggt cgatccggtt
    21661 gagcatgaac gggtcgaaca ccaccggcgt ctgacctcgg agggccggga ggcccgggtc
    21721 ctccgagagc agggtcgctc cggcgggcag ctcccctgcc agcgggtcag ggcggaaggg
    21781 agactcggca ccgaggacga tcttcccggc ctccttcagg tcctcgcgca tgtgaacggc
    21841 aacgcccgtg aacgtgaccc acacgagggc cacggccacc accgacgcga acacctcgtc
    21901 cccacgctct ccgaacgccg ccgttagctg tccggtgacc agcgccatca gcacgacgag
    21961 gtcgatgagc tggttggagc cggtcccgat gtccgcgaac actaccagca ggatcgcggc
    22021 ggcgaacagg aggccccagg cgaagacgct cggctccacc cgccgcaggg tcagcagccc
    22081 gaacgccgcc acagggagga ggacccattg ggccaccgcc ccctcgaaca cggcgtcgag
    22141 gaaccggagc ggagcggtcg cggcgtcgcc cacgctcagg atcccgcccg ccgcgaaccg
    22201 gaccaggttg tcgtacatcc gcccgtccga ggcaacgtgg aggagcccca gagacaccac
    22261 agtcaggccc acgaacgacc ccgcgaatac caggagcctg cggcggtctc ggggccacag
    22321 ccacacggcg agcgcgaggg gggcccacag cgcattgaac cgcgacagaa gggccagggt
    22381 gcacagcgcc ccggccccca ccgcccgggc gtcgctccga gtccgcagga ccaacgccac
    22441 cgcggcgagc tggaagagga ccggcaggac gtcgccgccc acgctcgtgc caccaagcag
    22501 cccggcgccc ccggccacca ccgcggcggt gagaccgaaa gcgacgggac ggggggagcc
    22561 gagccggacc aaggtgacgt agaggaggac caggagcgcg gccgtggcca ggagcgccac
    22621 cagcttgccc gaggtcaggt actcgccggt gacccgcgcc accagcccgt ggaggacgaa
    22681 cggaccaggc atgtaccgga cccccccata gtgagccccg tcgaagatct gcgggtacag
    22741 gccctccgcg tcgagatgct gggccagggc gatccagacg ccctggcggt gccggacctg
    22801 gtaccgatcc cccaggtgca ccacggccag gaacagccat gacgccacga ccccggcgct
    22861 ggtcaggatg agcgcgcctc gtagccaccg ggtcagtcgg gcggggagcc gcggaaaggc
    22921 gtcaccgacc gatggtccca cgcggggatc gtcgcgaagc atccggagca caggatagcc
    22981 ggggcaccga gaggggcgga agactcgcta tcctgagcgc agtgaggagg gagacggaga
    23041 ccgtggactc cgcccgtgcc gacgagccga gccgcgcgtc ggccgggtcc gtcgtccgcg
    23101 acctgttggt cacggtacgc ccgcggcagt ggatcaagaa cctcctcgtg ttcggcgccc
    23161 cggccaccgg gcgcgtcctc ttcgagcccg cggccctcgc ctcgacgacc ctcgccttcc
    23221 tggccttctg cctggtggct ggcgggacct acatcatcaa cgacatcgtc gacgcccctg
    23281 ccgacaggac ccaccccgtc aagcgccgcc ggcccatcgc cgccgagacc atctcctccc
    23341 gcctggccgc cgtggtggcc ttaatcctcc tcaccggcgg cctggccgtg gccttcgccg
    23401 gcggcgggct gccactcgtg gccgtcacgg gcggctacgt ggccctcacc ctggcctaca
    23461 cctactggct gcgcgacatc gtcttgctcg acatcgcggc catcgccgga ggcttcctcc
    23521 ttcgcgccgt ggccggcggg gtggccaccg gcgtcccgct ctccgagtgg ttcctcctcg
    23581 tggcctcgtt cgcctcgctg ttcctggccg cggggaagcg caccgccgag tacgtccggc
    23641 tgggcgacga tcgggggaag caccggccga gcttcgacga gtactcggag acctacctcc
    23701 ggttcatcca gtacagcgcg gccaccatca cgatcgccgc gtacgcccta tggtcgttcg
    23761 agggggagac gggcgggacg gcgtggtcgg gactctccat catccccttc gcgctaggca
    23821 tcttccggta cggcctcctg ctcgacaagg ggcacggggc ggcgccggag gacgctatcc
    23881 tgtccgatcc acccctgctc gggctcgggg tggcctgggt cctgctggtg gccgtgggcg
    23941 tctacgtggg ctgagaggga gcgatgccgc gggcgttggt gacgggaggc ggggggttcc
    24001 tcggatccca cctgtgcgag cgtctgctgg ccgaggggtg ggaggtcctc gcggtcgact
    24061 cgctcctcac cgggaacagg gacaacctca cgggcctcct cgggcatgac gggttccgct
    24121 tcgaacagcg cgacgtcacc gagctggagg aggacgaccc ggtcctcgag gaggccctcg
    24181 attgggtcct ccacctagcg tccccggcgt cgccggtcga ctaccttgag ttccccctgg
    24241 acaccctccg ggtgggctcg ctcgggacgc tgagactgct ggaggtggcc cgggcgaagg
    24301 gagcgtcgtt cttcctggcc tccacctcgg aggtctacgg cgaccccctg gtccacccgc
    24361 agcccgagac gtactggggc aacgtcaacc cgatcggccc gaggagcgtg tacgacgagg
    24421 ccaagcggtt cgccgaggac gccaccatgg cataccgtcg acagcacaac ctgaagattc
    24481 ggatcgtccg gatcttcaac acctacggcc cccggatgcg acgcggagac ggacgggccg
    24541 tccccacgtt catcgagcag gctctccgcg gcgagccgct cacggtccat gggaccggta
    24601 cccagagccg ctcgctctgc tacgtggacg atctgatcga gggcttctac cgcctgatcc
    24661 tgtccgacca gaccggcccc atgaacgtcg gcaaccccga ggagatcgcc atcgcagacc
    24721 tggccacgac gatccgggac atcgtcggca gcccctcgga ggtcgtcatg gtcgaccggc
    24781 ccgtggacga tccggagatg cggcggcccg acatcgagct ggcccgacgg gtcctcggct
    24841 gggagccgac ggtcccactc cgggacggcc tcacccgcac catcgactgg gcccggaccg
    24901 cctggcgctc cgaggtcggg tccgcggacg accgtccgtg acccggcgca cgggcatcct
    24961 ccgcatctcc cgggagatga aggcgtcgag tcccgccttc atcgtggggg aagcacgcag
    25021 cgggacaagc atcctgtacc ggaccctgat gaagcacacc tcgttccgca cgcacgagcc
    25081 gaaccttgtg gagacggaga tcttcgccca cctccgccgg gtcttcatgt tcgggcccga
    25141 ctacccggcg cccctgctcc ggtacatgct ggacgaccgg aaggcctacg gcgccttcct
    25201 ggcctcgatc cgccccctcc ggctggtgag cgcgctgaac atccccgtca acctcctgag
    25261 ccgcgggccg atgacctgga ccctccgcac aaacatggcc cacctcgtcc tccgcagctt
    25321 cttcttccac gcgcgcctgg cacgaggctg tcggcggctc gtggagaaga ccccgaccaa
    25381 taccccctac atccccgcgc tctcccggat cttccctcag gcccgcctgt tgtacatcca
    25441 ccgccacccc gtggacgtct acagctccta ccggcggagg ggacaggccg accctgacgc
    25501 cgcgtgggcc agatcgctta cccccgatgc cttctgcgag atgtacgcgc actcggccgc
    25561 tctcgccctc gactgggccg cggggcacca caacctcctc ctcgtctcct acgagcggtt
    25621 caccggctcg cccgaggtcg agctccgccg ggtgtgcgag ttcctggggg agccgttcga
    25681 ggccgccgtc ctcgaggagc ccaaccccga cccgggccgg tggaagggcg accccctcct
    25741 gtggggcccg atcgtccccg tgacgaagcg gtggcaggac tacatgaccg gggatgaagc
    25801 ggccctgatc gagcatcgtc tccatgggac catgcgacgg ctcgggtacg acgcggtcgg
    25861 gcccccggcg gcatcaggat gatccggtgc aaggcaagga tcgcgtgctt cccacccctt
    25921 tacccgaatc gcgtatcgaa tagggggggt gtagaagagc cggcgcggcg tactctgggc
    25981 tcaggaggag cccgagaacg tgggcgccgc tcgctgttgc tcctcaagat gaagcggctc
    26041 cctcaggcac ttcgaccttc ctagttattt agggctatgc ccatatcacc cctacggggc
    26101 atgccgggac ggccgtctcg ctgtcacact attggaggac tgatgcgaaa tccgtatatc
    26161 tcagacgcag tcatggcctt ccggccgaaa ttgcgcggcg aggtttgctc accgggcgtc
    26221 ggtggagaac atcctggtgc gcagctgacc gcgaccgtcc cgtcctggga gcctatctcg
    26281 gagtctatgt gaacggatcg atcaaacagg aagccgtgcc cggtaagggc gtggagaccg
    26341 gttccgcgcc tgcccagcgc cctaccctga ggctcgagcg ccgccgcacc gcccgcacgg
    26401 ccggcacggc gctgcccctc cgtcggcggg cccgattcgg cctcgtggtc gccgacgccg
    26461 tcgccctgct cggggccctc gcggtgatgg gaagcatccg gccggagatc caagggctca
    26521 gcgtgcacat cctggcgccg atggccgtcg ccgtggtcgc tcagatcgtc gtgttcgccg
    26581 ccgtcggcct gtaccgcctg tggcggatgc cggcctggga cgaggtccgc cggatggtgg
    26641 gggccacggc catctgggtg atcgtcgtgg ccatcgccac tccatggtgg agcggcgcgg
    26701 tctcgagatc attcctcctg tacacctggc tgatcgccct ggccttcctg ttcccgatgc
    26761 ggggggtggt ccgctgggcc ctgagccggg ccaagaccaa ggaggaggcg gccctgcgga
    26821 ctgcggtggt cgggcccaac ggcgaggccg gctcgatggt cagcgggctc tccggatccg
    26881 gggagatcgc accggtcggg tacatcgcca tgaacccggg ggacacccac tcgaacgggc
    26941 tgccggtgct gggcaccctc caggacctcg ggtcgatcat cgtcgacaag gagatcgagt
    27001 gcctgctcct tccggcgacg gggctctccg aggacgagat cctgcgcgtc tcgagagcct
    27061 gtcggcgggc gagcatcgag ttgaaggtgt ggtccgagct gcccgacatc ctcggatcac
    27121 ggctggcgat ccagccgatg gacggccggg tggccctggc gcttgagccg gtccacttca
    27181 cctccaccca ggcggcgctg aagcggtcgt ttgatctcgt gaccgcctcc ctcagcctcc
    27241 tggtgctgct gcccttcctg gccctgatcg ggctggcgat ccggctcacc tcccggggcc
    27301 cggtgctgtt ccgacagccc cgggtcacca aggacggccg ggtgttcacg atgtacaagt
    27361 tccgcaccat ggtgagcgag ctccaggagg ccctggagat cgacctcacc cagccattct
    27421 tcaagatgag ggaggatccc cgtctcacca aggtgggacg gatgctccgc agcctcagtc
    27481 tcgatgagct cccccagctg ctgaacgtga tcctcgggga catgagcatg gtgggccctc
    27541 ggcccctccc ggtcgagcag atcgaggcca acgcggagtt cttcgccccg cgccacgagg
    27601 tccgtggagg catcacgggc tggtggcaga tctcgggacg cagcgccctc gactcccggc
    27661 gggccctccg tcgggacctc tggtacatcg agaactggtc gctgggcctc gacgtctaca
    27721 tcttgatccg cacgctcgga gctgtgatcg cacgacgagg ggcctggtaa ggagtagagg
    27781 atgaagattg gagtcatcgg aacgggacac gttggtctgg tggcgtgtgt cacctacgcc
    27841 gagatgggcc atcaggtggt cggggtcgac gtggacgagg agcgcgtcga ccagctgggg
    27901 cgtggcatcg cgcccttctt cgagccgggg ctggtcgagc tcctgcagcg gcacctggcc
    27961 ggcgggagcc tctccttcag cacggccatc gccgacgcgg tccggggcgc tgaggtcgtg
    28021 ttcatctgcg tgggcacgcc tagccgagcg accggcgagg ccaacctcct ggcggtggaa
    28081 cgggtcgccc gggaggtcgc cgtgcactcg gatcaggcgg ccggaggacg gctggtcctc
    28141 gtccagaagt cgaccgttcc cgcgggaacg gccacccggg tccgcagggc gctccgcatg
    28201 gcgaaccggg acgcgggggc ccgggtcagc gtggcctcga acccggagtt cctccgggag
    28261 gggcaggcga tccgggactc cctggagccc gaccggatcc ttgtgggggc cgactccgtg
    28321 tacgcattcg aggcgatgcg gcggctgtac aagtccctca tcgacaatgg caccgagtac
    28381 atcgagaccg acgtgcccac ggccgagctg gccaagcacg cgtccaacgc gttcctggcg
    28441 ctgaagatct cctacgcgaa cgcgctggcc cgcctgtgcg agcgctcggg agccgacgtc
    28501 acccgcgtgg ccgacatcat ggggtcggat ccgcggatcg gacgggcgtt cctggacgcc
    28561 gggatggggt acggcgggtt ctgcttcccc aaggacctcc aagccttcga gcacctctcc
    28621 gacagcctcg ggtacgactt cccgctcctg cgggaggtgg cccggctcaa caacgaggcc
    28681 ctggtggcca ccctgaccaa gatcaaggag ggggtgtgga acctcgaaga caagcgcgtc
    28741 gctctgctcg ggctgtcgtt caagcccggg accgacgacg tgcgattcgc tcccgccctc
    28801 gagctggccc agcgcctcct ggacgagggg gccaccgtgg tcggctacga cccgcaggcc
    28861 atgacgaacg ccaagcagga ggttccgggg ctggagctcg cggccgaccc ctacgaggcc
    28921 gcctcaggcg cgcattgcct cgtactatgc actcagtggc cggagttccg agacctcgac
    28981 ctgcatcatc tgcggcgcgt catgaccgat cccctggtcg tggacggccg gaacctcttc
    29041 gacgcgcgcc agatggccgc ggcggggttc ctgtacttct cggtggggcg gccgcccggc
    29101 ggcctggaga tcgacctgcg cgacgtgggg gagacccagc aggccgacgc ctcctcgtag
    29161 ccccctgccg atgcgccgac cgccggagga tgcgaggatg acgcgcgcgc cggaacgggt
    29221 ccccgatccc actcgggagt gggcgaccct gccgacctct cgcgcccccc ggtggttcct
    29281 cccacgacga cgggggacgg ccaccaggcg gagcttcatg atctaccacc cggtcaccct
    29341 ccggagccgc gtgggctggg aggcggcccg acgcctggcg gccgtgggcg cgctggagct
    29401 cctccggccc tcgaacatgc taccccgcga ggtctgggag cccgtcgccg gggtgatccc
    29461 gcagggcggc gccctggcgg tggccagagc caaccacccc ggccggttcc tggccttggt
    29521 ggtgggggcc gacggcgacc tgctcgcctt cctcaaggtg gcccgggaca gcgtggggtc
    29581 ggacgtcctc gagcgcgagc ggcgggccct ggagacctac gggccactcc tcccccctcc
    29641 cttgttcacc ccgcgggtcc tggacggcca ggcggggagc ctgctgctgg aggcgatcga
    29701 gtggaggagc cgggccatcc cctgggggct ccccaccgac gtcgcccacg cgatgggaag
    29761 attctacgcg gcctcggtct cggaagggtc gcagggggtc acgcaggggg acttcacccc
    29821 gtggaacctc ctgcgagccc cccacggctg ggcgctcgtc gattgggaga gcgcggagag
    29881 cggcgtgccg ccattctttg acttgttcca cttcttcgtg cagtccagcg ccgagctcag
    29941 gcggcccagc aagcgggccg tgatcgaggg gctggacctg cgggggtggg tcggtgcgtg
    30001 cgtggaggcc tactccgccg gggcgggggt cgatccgcgg gcggcgaagg atctcttcgg
    30061 cgagtacctg cggttcagcg ccgcgcgcgt gtccgcgggc gctcccccga gggcccggcg
    30121 catccggcgc gagctggccg cgcgcctcgg agcggtgagg acgggggcgc cccagcccga
    30181 tgcggcagga ggtgggccat gaggtcctgg agtggcgtct acgtcgtcct ggccggaccc
    30241 gacggctcag gcaagtccac cctagcctcg aggctcgcgg cggcggcccg gcggtcgggg
    30301 aggccggctc gccacctgca ctggaggccg gggctgctgc cccaggccgg gagcctggtc
    30361 ggcagggctc caggcgaccc gtccacgccg cacgcggagg agccgcaggg ccgggtgctc
    30421 tcgctcctcc ggcttgtgta ctactgggcg gacttcctgc tggggacgtg gctgcgggtg
    30481 cagccgatca cggcccgcgg agggctggtg gtgatggagc gagggtggtg ggacatcgcc
    30541 gtggatccgc ggcggtaccg cctccgggtc ggcgcccggg tgatccgggc gctgggacgg
    30601 ctgctgccgg ccccggacct ggtgctcgtg ctcagggcct ctcccgaaat tctcgcccag
    30661 cgcaaggagg agctcccgac ctcggagctg gcgcgccaga acgacagatg ggacgagatc
    30721 cggttcccac agcggatcgc caagatcgtc ctcgacgcca cccgtagcca ggacgacctc
    30781 gtcgccgacg cggcggccca catggccacg gtgtcggggg gcgaccccgt cggggccggg
    30841 ggggtggagt agccgtgccc cggttcctcg ccttcgcata cggctgtgag cccgacaagg
    30901 gctccgagcc cggcgcggga tggatctgga gccggatgct ggcccgcatc ggcgagacct
    30961 gggtgatcac gcgggagaac aaccggccct cgatcgaggc cgcactgccc tcgaccccgg
    31021 agcgggacaa cctccggttc gtgtacgtgg acctgccgga gcgggccagg ttctggaagc
    31081 ggggcatgcg tggcgccagg ctctactacg tcctgtggca ggcggcggcg gtgtccaggg
    31141 cccgccgact tgtccgggag tccgagttcg acgcggtatg gcatctcacc tgggcgaacg
    31201 cctggatcgg gtccatggcg gccctcctcc cccgcccgtt catctacggc ccggtcggcg
    31261 gcggcgtgag gatgcattgg ggcttcacgt ccgtggtggg ctttcgtggc acgatgtatg
    31321 aggtcttccg cgcgctggcc aggggcacgg cacgatactt caatcccatt gcacggctcg
    31381 cctggaggaa ggcgcggctg atcctcgtcc tgaacgagga cacccggaac tggcttccac
    31441 gacgacatcg cgacagggca gttgtcttcc cgcacgccgt cctggaccga ccgccacgcc
    31501 cgactgccga ctcacgaagt cccggccgca acgtcgcgct gttcgccgga cggctggtcc
    31561 cttggaaggg aggggctctc gcaattcaag cgcttagtct gcttcctgag tggcggctgc
    31621 tgttctgtgg tggaggcccg gacgaggctc gcctgaagct cctggcggca cgctcagacg
    31681 tcgaggaacg agtggacttc ctgggatgga tgacgagaga tgaagtgacc tcctggatgc
    31741 gaaaggcgga cgtcttcctg ttccccagcc ttcacgacga tgcaccgttc gctgtcgctg
    31801 aggcactggc ggaggggctt ccagtcgtct gcctcgctcg cggtggagcg gccgccattg
    31861 cgggacgcgg cgtgccaacc gcatccgtgg ccagcactgt tgcgggattg gccgaagctg
    31921 ttcgggagtc aagcaatgtg gaagtcccag ctttcccgga catcgattct cgcgctcaaa
    31981 ggctcgcgga gctccttcgc gagcggttcc cctcctgggg catacaggta ccggaagaga
    32041 cgaaccatcc agaggcgatc catggaccca ccggcctgag gggtgatcca cctgctgagt
    32101 cctgatctca ggcgtgcggg gagcgagacg ctccttgtga tctccggact cgctctcgca
    32161 gcggcggcag gagcctacag cgtcgggcat ccacagcgag cgcttgtgtt cggcccgctg
    32221 ttcgtcctcg ggctcatcct tctggacctc aatggacgca agggaacgct cgtcgaccgc
    32281 ttcgggtgga tcccctgggc gtgggtcgct ctgctcttgg tgaccgacct tcgcctcatc
    32341 aatgcggatc cgctggccgt cgcggccggt gctgcgaatg ttcagcacct catccagatc
    32401 gcggcctacg cgctcgtcac cgttctggtc gtccgagcac ggagcaccat catcggcttg
    32461 ttcccgcgac cggtccccaa gggcctcctg ctggcgttcc cgctggtcgc cgtggcgtcc
    32521 gcggcctggt cgctcatccc catctatacg gtcgtccggg cactgcagct ggtcgtgctg
    32581 gcgctgctgg cgctcctcgt ggtccggatc tgggcgagcg atccagacgc cggggaggag
    32641 atcttcgcca ggatgcttcg gctttttgtc caggtcgtgt cgatcctggc ggtcatcggt
    32701 ctggccaccg cggagaacct cggcgatcgg ttcacgtggc tgggcatgca ccccggagtc
    32761 acggcgacct acacgggggc cgccctgctc atcctcatcc tcggcggacg gtcctggttg
    32821 cgcttccccg gctacaccta ctggccacga ctgctcctgt tcggcgtggt gatctacctc
    32881 gcacagaccc ggagcgtcct ggccggcatc gtgatcggca taggcgtggg cctctggttc
    32941 aagggtcggg acaaacccat cgctcggtac ctaggggtcg cctactacct catcggaggg
    33001 cttctgttcg tcctggccac ctcccttcag ctcaccggct acctgacacg aggagagacg
    33061 gctgaatcga tcgcgagcct gagcggtcgg atcccgctct gggagttcgc catcggccag
    33121 ctggaatcgg tccaggaatg ggcactgggg ttcggctacg gatcgagccg gcttgtcctc
    33181 ctggcgtcgc ccgggttcca gtgggccggc tcggctcaca gcacatgggt ggaggtcctg
    33241 ctggggatcg gagtcgttgg tttcgtggca accgcggcca caattgtggc cctcgtcttc
    33301 acactttcca gaagtcaggc gttcggccgg cctaacgtca tcgccctctc gatcctcgcg
    33361 ttcttcctca tggcctcccc gatctcagaa gggatggctc tgccggggat cggcttcggc
    33421 ttcctggccc tgctccacgt ccccgccctc ggcaagcgag cgctccggct gccgcgacat
    33481 cgtcctgatc gcgcacgggc agacgtccgt acccgcggat gaaaccgttc ttttctggcg
    33541 tgaggaaact gacacgctcc gcacgatcga tcggcgggcg attcggctgg ggagtggcgg
    33601 accaggcgct gtcgagcctg accaacttcg tgatcggctt cctcgcagct cgaggcctct
    33661 ctcaatcgga gttcggtgcc ttcgccgtag tcttcgccgt cttcctgatc gggctggggg
    33721 tgaccagagc gatcaacagc gagcccttcg tgatcaggta ctcggccgta tccgctgaca
    33781 gatggagaca tggcgccaca tgggccaccg gcacggcact cgccgtcgga gcgctcggtg
    33841 gcgtggcgtg cctcgtcgcc ggcatcatcg ccgggggcgc tctcgggagc ggcctcgtag
    33901 ctgttggtgt cgccctcccc ggtctgatgc tccaggacac gtggcggttc accttcttcg
    33961 ccgcccgacg aggccgctcc gccttcacga acgacgcggc ctgggtactt gctctcgcgg
    34021 ctggtgtggc cctcctggcc gtcgccgatt cctggtcggt cggatcgctc gtcctggcgt
    34081 ggggcggggc cggcacactc gccggagtca tcgggatcct ccaagccgga gtggttccca
    34141 ccccgaaccg gagcctggac tggcttcgcg atcaacggga tctggccccc aggtttctcg
    34201 gcgagttcac cgtcacgacg ctcgtgagcc agatcaccgt gttcggactg ggaatccttg
    34261 cgggactcgc ggacgtcggc gcgctgcgag cagggacact cctgctgggg cccctgaacg
    34321 tcctcttcct gggggtcggc atcgtcgcgg ttccggaggg agtcagggcg ctcgtctcgg
    34381 cgaaggagaa actcctgcgt gtctgtcgaa ggctatcgat caccctcgcc tcgttcgcac
    34441 tagccttcgg cacagcgatg tggctgctcc cggatcggat cggcgttgcc ctcattcggg
    34501 agaactggca tgcggggcac tccgtgatca tccccctcac gctcgtcgtc gccttctcgg
    34561 gcatcctctt gggggcgggg atcggactac gatcgctggc ggcggcgaag ctgagcttcc
    34621 gggcgagact cctcgtcgcg ccgatctcgt tggtggccgg cctcggggga gcggtgctgg
    34681 ccggcgcggt aggagctgcc tgggggatgg ccttcgcttt cgtcgtggga gccatcatct
    34741 ggtggcgata cttcctgaag ggactccgag cgcacgtcgt cggcccaccc gacgaacgag
    34801 cggcgaccga cgtctatccc gtcatccacg gcgatgcgta ggctctcgat cctgaccccg
    34861 tccttcaatt acgcgtggtg tatcgaggat gccctgaaga gtgtggcgca agctgcgggc
    34921 tttgtccccg gcggatgggc tgtcgagcac gtcgtggtcg atgacgcgtc caccgacgag
    34981 agcggtgaga tccttgagcg atggcgagat cgggtcgtcc tggagcgcca gaccgagaat
    35041 cagggacagt cgcggacgct gaaccgagcc cttgccctgt cctcgggtga gtggatcggc
    35101 tggctgaact ccgatgactt cttcctcccc ctgtcgctgg ccgatgcgtc gcgtgccttc
    35161 gacgacgagg atgccgacgt catatacgga gatgtggccc tgctcgacgg gtcggcacgg
    35221 ttgctccgac tcatgaccga gcatccgttc tcgctgtgga cgctcaggtg gtggggtacc
    35281 tatctcgcgg ttggcacggt cttcctgcgc cgcgaccttg tctcgcgcct ccgttggcgc
    35341 gaggacctca gcctcctgct tgattgggac ctctggctga gggcggcgga gtcgggggct
    35401 cgtttccggt atctcccccg gccgctggcc gcggtccgcc gacaccctgg gcaggagagt
    35461 cgccaggatc ggcccgatcg actcaccgag aaggccaccg tccgccggga gcatcggctc
    35521 ccatccagac cctggatgtg gcgcgccgct cagcgtgtcg cggcgttgac ccatggcgtt
    35581 cagaagctgg cggcaggagg ctacgccagg gagcagcggg ccggtgctgt ccgaggacga
    35641 ccgatgcgct ggttcgaaga tccagaagcg aggtccacgg tcgacagtct ctacgagctt
    35701 gtgtacggag gccgctctga tggaaccctc ccttccaggg agggatgatg cggatcttga
    35761 tcctgcactc ccggtatcga tccgggcccg cctccgggga gaatcgtgtc gtggaggatg
    35821 aggcgaggct cctctcggag agtggtcacg acgtcgaggt ctgggaccca agcgtcggca
    35881 ccccttccgg catcaggctt ctgcaagcgg gtgctcgagt ggtgtggggg cgtgccgcgg
    35941 ctgcggaggt ccgcacgcgc gtggcccggt tccgccccga cgtcgtccac gtccacaacc
    36001 tcttcccctc gctctcgccc tccgtgctga ggacggtcac gggacgcgcc ccgctggtca
    36061 tgacgctgca caactaccgg ctccactgtc tccccggcgt cttcctccga gacggacggg
    36121 tctgcgagga ttgcctcggg cggaccccct ggcccggggt tctccacggg tgctatcaag
    36181 gctcggtgcc agccagcttc gcgctcgctt cctctctcgt cctgcaccga cgacttcgga
    36241 ccttcgatgg tgtcgatctc tacctggcga tcagcgagtt cgtccggagg aagaacgtcg
    36301 agggagggct ccgaggggat cgcatcatcg tgaagcccca tttcgtgtgg ggtggcgagg
    36361 tcaggcaggg accgggcgac tacttcctct atctgggacg cctgtcaccc gagaaggggg
    36421 tcgctacgct catcgaggca tggcgtgtcg ttgaggccag actcctcgtc gtcggggagg
    36481 ggccagaggc ctcggctctc cgcgagcggg cgccctcgaa cgtcgagttc cgctcgacgg
    36541 tcccgcctga gaaggcgcga gctctgttgg ggtcggccag ggccatcttg gttccctcgc
    36601 tctcccacga gggcgcgggg agagtggtgc tggaagccta cgcggcgggc gtccctgccc
    36661 tcgccagtcg ctcgggaggc ctgcctgagg tcgtgtccga cggtgagacc gggctcctcc
    36721 tccccgtggg agatccccac gcatgggcag agggtgtgag ccgtctcctg agcgatgccg
    36781 agtccgagcg gatgggccgg gcagccttcg ggctgtggca acgagcctac agtcctcaat
    36841 caggtcgggg gcgcctggag gacgcctatc ggtccgtggt ggggacgagg gccaccaggg
    36901 acgggtgaag cgaggcgcgt tgggatgcgg atagcgcatt tcctcctcgg ccggagcaac
    36961 ccggagagct ccaacggggt ggacaagacc gtcttcttcc tgagcaaggc ccaggcggaa
    37021 ctcgggcatg acgtgtccgt cttgtccatc agcgacctcc ctccgatccc cgttccgggc
    37081 gtcacggtgg gaaccttcgc tccgaggcgg ctcccgagct tgcggccctt cgacaaggag
    37141 gggcgagcac gaccccggaa tcccttccga ctcccggacg ggatgtggga cgacatggtg
    37201 gggcgccacc cccacatcgt tcacttccat tcggtccaca tcccggaggc actgtggctg
    37261 gcagacagac tgaggcgatc cgaaatccct tattgtgtct cgcctcacgg aggtctcctc
    37321 actaccgcgc agcgacatcg ccgacgcctc aggaagcagc tcttctctac gctgctcgag
    37381 cgtcggtatc tccgaagagc gagcctcatc catgccgtga gccaagccga ggctgaggag
    37441 ctccgccgac agaatctcgg acctccggtc gtcgtggccc acaattgcat cgacgccagc
    37501 gcggtcccgg atgacgtcga tccgggtccc gtcatcgagc gattcccaca gctccagggc
    37561 aagcgggtcc tcggctatct cggtcgtctc gacaccgggc cgaaggggct ggatctgctg
    37621 ctgcggggct gggcgaggat cgccgatccc tcgaacgagg tcctgctcct cgtcggaccc
    37681 gactggcgcg ggggtcggat gaggttggag cgcctggcat ccggactcgg ggtgaacggc
    37741 ggcgtggtct tcgcgcccgc ggtgtcggga cgagagaagt gggcctactt cgcggcgatg
    37801 gacgtcttcg tggcgccgtc ccgttgggaa gcagccccgt tcaccgtcct cgaggccatg
    37861 gcgacgagga ccgccgtcgt cgccaccccg gcgagcgatc cggacggtct gatccgagcg
    37921 agcgaggccg gatgggtcgt gcaggcggag gtggaggcgc tcgctcgagc gctgaagacc
    37981 gtcacgcaga cgtccggcga gcagctccga ctgatgggaa cgaaggcccg tcgcctcatc
    38041 gagcgggagt atcgctggaa cgacgccgcc gagatcctca ccgaggcgta cgaggatgcc
    38101 actcgggggt gaccggctcc tcacgagggc gcgctacgcg cccgctgggg atctccggcc
    38161 cagctcggga gaaaccaacc ggaccatcca gaggggtgtg tgctcctgcc accatcccac
    38221 cacgtcgtac gctcccagaa ccttgtcccg cagcctctcg acatcatccc cgtcgacgaa
    38281 gaatcggggg atccgcagca gggggtccga ggcccgaacc tgtcggtaca tcgccgccag
    38341 gccgatcgca tggccatgct gttccacgat cctctccacg ctcggcgtga agtggaccct
    38401 cggcctgccg aacggatagg cgaagagatc gacgtctgtt ccgaggtgct ccgagagcaa
    38461 cgatcgagcc cgacccacct cctcgtcgac ctcggcggga gcaaggcgag cgaggttccg
    38521 gtgcgagtag gtgtggttcc cgatctccat gcccgcggac gtcatctctc gcagctgtga
    38581 ccagctcatg aagcgaaggt cgccggggtc tcgacgaacg atcgtcccga accgctctct
    38641 cacgtcgcgg tctccatcga tcaggccggc ggtcacgaag aacgtggctg ggagcccgtg
    38701 ccgggccagc aggggcatcg catgcgcatg gttgtcctcg tacccatcgt cgaacgtgag
    38761 cgcgacgagg ggtttggcgg gcggctcacc ggagcgagag gcgatcaggt cccgcagggg
    38821 aaccacctcg cattcgtccc gcagccactc gaggtgcgat tcgaagagct cggggggcga
    38881 ggagaagtcc caggaggacg ggtggatcga atggtagacg aggacgaccc gccgatgggg
    38941 gcgctcgaac aggcggccca cccgtaccgc cccccgcttg accgatctcc tccacctcat
    39001 cgagaccgct cgacgtcgcc gcgcttcatg accgtcgtga ggccggcaag gctgtcacgg
    39061 tgtggttggc cctcacatcc tccagccagg tggggaccga gatccggtgc gccgcgccga
    39121 tggccttcag cacgtcgcac tgccgcatgc cccagcccct cgacgcggcc cgcgagagga
    39181 ggaaccgtgg gtgggccaga cccctcatca tcatgacggc ctccgcgtgg atgaggggga
    39241 accgttccca tcggccccga gcggctgctc ggatcgccct gaccatcggt gccctgggca
    39301 gcccgagtcg cgtgatcgcc tcctccgtcc cttcccagtc ccctgccatc ttctgcagga
    39361 ccccgtactt ctccagcccc tgccccatcc gacgaccacc ccatctgatc cccttgtaga
    39421 ggaacagcag gagaccttcc gccccctcac ggatccgtct gaacccgcgc tggtccatca
    39481 ccatgagcgg taccagatcc tccgccgtga acaggcgagt ccccctgaac gatgattcgg
    39541 cggcgacgtc gagctgcgcc cacgtcgact cgctcaccgc gatcagcgca aggacaccct
    39601 cggcatgcac gcactcgatc acccactgga ggccttgtcc ttcggcccag tcgtaaaaga
    39661 gggtggcgag ctcggcatgg tcccgagtcg gagccgccga gtcaacatcg ccactcccgg
    39721 ccagcgcccg gtccgtattc ttccagatcc cccacgactc actcgatccg gtcagctgtc
    39781 gcagcagggg gagccattcg gccgacggca tcgtcagttc gtggccgctc acgtctcgga
    39841 gcccagggag ccgcacgccg gaggaggctt caccacgagg ctagaatagc caggatttga
    39901 ggaaccggcc ggaccaacgc cgaaccaggt gtgaccacga gggacccccg ggacgaggtt
    39961 tgcggatgtg agcgttgtga tggtgacggg gtcggggggg ctggtcggct ccgaggcgtg
    40021 cctgtacttc ggcgacctgg ggctcgacgt cgtgggggtc gacaacgaca tgcgggccac
    40081 gttcttcggg gacgaggcgt cgacggcgtg gaacgtcgac cgcctccggc agcagctcgg
    40141 ggactcctac cgccaccacg acctcgacgt ccgcgaccgc gacggggtgc tcgatctctt
    40201 cgcccgtcac ggttcctcca tcgagctggt gatccacgcc gccgcccagc cgtcccacga
    40261 ctgggccgcc cgggagccgt tcacggactt cgacatcaac gcggtgggga cgctgaacgt
    40321 gctcgaggcc accaggctcc acgctcccga ggcggtgttc atcttcacct cgaccaacaa
    40381 ggtctacggg gacgcgccca accgactgcc gctgcaggag ctggagaagc ggtacgagat
    40441 cgaagaggac cacccctacg tcgacggcat ctccgaggac atgtcgatcg accgctccct
    40501 ccacagcctg ttcggcgcgt ccaaggtcgc cgccgacgtg ctggtccagg agtacgggcg
    40561 gtacttcggg atgcggacgg cctgcttccg cggcggaacc ctcaccggcc agctccactc
    40621 cgccacc
//
LOCUS       DASUTW010000061         6807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1832250, whole genome shotgun sequence.
ACCESSION   DASUTW010000061 DASUTW010000000
VERSION     DASUTW010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6807
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832250"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1680
                     /locus_tag="VF058_02180"
     CDS             <1..1680
                     /locus_tag="VF058_02180"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6207143.1"
                     /translation="LRRASVLGLGFEASLAEELSADPAGPEAWERLDEFVERENGTLR
                     FRHALIREAAYEGLPYRVRRSLHASAAESIERGTGDQPEEQAELLSLHFFNAGRYPEA
                     WRYSTVAGDQARSMYANVEAADLYQRALDAARRAGGIPARRIGEVLEALGDVKSRSGL
                     YEDAARAYREARRRGDGGAVRDAQLMLREAETLHWLGQFPRAMGWVTRALRSLEGLPA
                     EETAATVAKLSSLYALCRFRQGRFREAARWAQRAVEEAERAGDKAALGEAYRVLDMVE
                     WSSGGTAGDTYGRVALGLFEELGDRSMQANLLNNLGVRAVLEARWDEALELLRRAEES
                     FSRIGHSQGVIIAAFNRGDLLVRRGQVDEAERHLQEALRMSRAVGDREGVAFATRELG
                     RAAAKAGRRDDALRLLESARAAFTELGARNEALETDAAIAEARLLAGDWSGALELATA
                     SISRAATIEGEPLLPILHRVRGYALLVGGRPEEARAALEEAVEIGRAQGAEDEVAFAL
                     AALAQAARALGDPEAAGFDREARELKGRLGVAFMPPIRAGLTSSADPRRRG"
     gene            complement(1646..2584)
                     /locus_tag="VF058_02185"
     CDS             complement(1646..2584)
                     /locus_tag="VF058_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207144.1"
                     /translation="MLRIGSSRMHGLQRAWVALATAVVVTGVVAGGTAMGAPPGSTKL
                     VITAVTDQGTGLPVPIAGQPFSVAVELRDQSDNLVTTSKKIDVTLSVSEGMLQGTTTG
                     TILKDQSGTTISGVIYPDTANVTLFASAKQIETGSWFLSVQKNAVAFGTSPAAFTVST
                     CGPGDPSPANPKCIVAEFHNGAPSGYAAHGSCLGYPGVQEGTCPSAETAADVSLISLI
                     SDLSGLYTKTDPLKVSWFCDESACPRGTGGPTSGNPFTTLFVEVNGSFVASPACPSKG
                     TIGPDQKFCTDYVQSSGLPGGDLLLVILFDGDPRWM"
     gene            2726..3490
                     /locus_tag="VF058_02190"
     CDS             2726..3490
                     /locus_tag="VF058_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX6207145.1"
                     /translation="MLDAELGLANELIDRAASIAMPLFRRGPEVRVKDDATPVTQADL
                     EIEEMVREQIGKRFPSDAVLGEERGGSIRDGPRVWVVDPIDGTKNFAAGIQVWGTLLA
                     LFEGGEPVLGVVGAPALGERYEAVRGQGATLNGTLIRASDVGSLDPAAVCHYATEAWL
                     EGPYEAALRDLDRAAYRSVGFTDFWGHCLVARGSVEVALEPELRIWDWGALKVLVEEA
                     GGRMTTFQGEPVSDRSSVLSTNGMLHQTVLEILTTD"
     gene            3528..>6807
                     /locus_tag="VF058_02195"
     CDS             3528..>6807
                     /locus_tag="VF058_02195"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6207146.1"
                     /translation="MSDSPRFHPLVEGWFAETLGEPTAMQAEGWRRIAAGRDTLVAAP
                     TGSGKTLAAFLWAIDELVRRAEAGTLEDETSVVYVSPLKALGNDIQRNLRDPLEAIRE
                     RAAERGLELPEIRVLVRSGDTPQSERQAMIRKPPHILVTTPESLFIMLTAERSRGFLR
                     SARTVIVDEIHAVAGDKRGAHLALSLERLDRLAEQPLQRIGLSATQKPIEEVGRLLVG
                     NGRLRDDGSPDCEIVDAGHRREWDLSVEISDAPLGPIATHELRAEMYDRIAELSKQHR
                     TTLVFVDTRRLAERVTHALEERLGEEHVAPHHGSLSREIRLRAERGLKEGSLRVVVAT
                     ASLELGIDIGHVDLVCHVGAPRSIATLLQRIGRSGHFLGGTPKGAIFPMTRDELLQTA
                     AAIRAARAGELDRVIFPREPLDVLAQQIVAIAAAEDVGEDELFALARRAFHYRDLSRE
                     DFDEVVEMLSEGVAGRHGRRSAHLHRDRVNSVLKGRRGARLAAITSGGAIPDIGDYDV
                     VEEPAGTFVGTVNEDFAIESMAGDIFLLGNTSWRIRRVEAGKVRVENAHGLPPTIPFW
                     IGEAPGRTEELSTAVSDLRAEVASRLEDPEAAARWLVEETGIPEEGAEQIVEHLSEGL
                     ALLGAIPTKDTVVAERFFDESGGMQLIIHAPFGARINRAWGLSLRKKFCVQFDFELQA
                     AATDDAILLSLGEQHSFPLDSVAGYLKPPTLRNTLIQAMLPSPMFANRWRWNATRALA
                     LLRHSGGRKVPVPIQRMRAEDLLSAVFPEQVMCQDNRMGDIDPPDHPIVNETVKDCLH
                     EAMDLDGLAEIVERIGGGEIRFVVAETPAPSVFSHEVLNAGPFAFLDDAPLEERRARA
                     VSLRRVDPDLQDGLGALDAEAVAAVREEAMPEVRDADELHDLLLSLGVLPLEAGWVEM
                     AEELRAAGRATVAHWNGRSAWLAAERLAKVRAAIPDLRLEDDLPLVGDESPSHDEAVR
                     DVVHGWMLVAGPVTAAALSERIGLPEPDVDRALLGLEASGIVLRGRFTGAPELEWCER
                     RLLSRIHRRTIRRLRAEIEPVDPAALMRFLLRWQHIQPGTQLHGREGMLRVLEQLQGF
                     ELPAP"
BASE COUNT          934 a         2342 c         2597 g          934 t
ORIGIN      
        1 ctgcggcggg cctcggtcct gggactgggg ttcgaggcca gcctggccga ggagctctcg
       61 gccgatcccg cgggtcccga ggcctgggag cggctggacg agttcgtcga gcgggagaac
      121 ggcacgctcc ggttccggca cgccctcatc cgcgaggccg cctacgaggg cctgccctac
      181 cgcgtccggc gctcgctcca tgcgtcggcc gcggagagca tcgagcgggg gacgggcgac
      241 cagcccgagg agcaggccga gctcctgtcg ctccacttct tcaacgcggg gaggtacccg
      301 gaggcgtggc gctactcgac cgtggccggg gaccaggccc ggtcgatgta cgcgaacgtc
      361 gaggcggccg acctctatca gcgggcgctg gacgccgctc gccgggccgg ggggatcccc
      421 gctcggcgca tcggcgaggt gctggaggct ctgggcgacg tgaagagccg ctcggggctg
      481 tacgaggatg cggcccgggc ctaccgggag gcacggcggc gaggcgacgg gggagcggtc
      541 cgggatgccc agctgatgct gagggaggcg gagaccctcc actggctggg gcagttcccg
      601 cgggccatgg ggtgggtgac cagggccctc cgctcgcttg agggcctccc ggccgaggag
      661 accgcggcca cggtggccaa gctctcatcg ctctacgcgc tctgccggtt ccggcagggg
      721 cggttccgcg aggccgcgcg atgggcccag cgggcggtcg aggaggccga gcgggcgggc
      781 gacaaggcgg cgctgggcga ggcctaccgg gtcctggaca tggtcgagtg gtcgtccggc
      841 gggacggccg gggacaccta cgggcgggtg gcgctcggcc tcttcgagga gctcggcgac
      901 cgctccatgc aggccaacct gctgaacaac ctcggggtcc gggccgtcct ggaggcccgc
      961 tgggacgaag ccttggagct gctgcggcgc gcggaggaga gcttctcgcg gatcgggcac
     1021 tcacagggcg tgatcatcgc cgcgttcaac cggggggacc tgctggtgcg gcgggggcag
     1081 gtcgacgagg cggagcggca cctgcaggag gcccttcgga tgtcgcgcgc cgtgggagac
     1141 cgggagggcg tggccttcgc cacgagggag ctggggaggg ccgccgcgaa ggcggggcgc
     1201 cgcgacgacg ccctccgcct gctggagagc gcccgggcgg ccttcaccga gctcggggcg
     1261 aggaacgagg ccctggagac cgacgcggcc atcgcggagg cccgtctgct ggccggcgac
     1321 tggtccggcg cgctggagct cgccaccgca tcgatctccc gcgcggcgac gatcgagggg
     1381 gagcccctcc tccccatcct ccaccgggtg cgagggtacg ccctgctggt gggcggccgc
     1441 cccgaggagg ctcgggccgc gctcgaggaa gcggtcgaga tcggtcgggc tcagggagca
     1501 gaggacgagg tggccttcgc cctggccgcg ctggcccagg cggcccgagc tctcggcgac
     1561 ccagaggcgg ccgggttcga ccgagaggcc cgagagctca aggggcgcct gggcgtcgcg
     1621 ttcatgcccc cgatccgcgc ggggctcaca tccagcgcgg atccccgtcg aagaggatga
     1681 ccaggaggag gtctcccccc ggcaggcccg agctctgcac gtagtcggtg cagaacttct
     1741 gatcgggccc gatcgtcccc ttcgagggac aggccggcga ggccacgaac gagccgttca
     1801 cctcgacgaa cagcgtggtg aacgggttgc ccgaggtcgg gccgccggta cccctggggc
     1861 aggccgactc atcgcagaac cacgagacct tgagcgggtc ggtcttcgtg tagagaccac
     1921 tcaggtccga gatcagcgag atcagcgaga cgtccgccgc ggtctccgcg ctcgggcagg
     1981 tcccctcctg aacgcccgga tacccgaggc acgagccgtg cgccgcgtag ccggagggag
     2041 ccccgttgtg gaactccgcg acgatgcact tggggttggc cgggctggga tcgcccgggc
     2101 cgcaggtgga gacggtgaag gcggccggcg aggtcccgaa cgccaccgcg ttcttctgga
     2161 cgctgaggaa ccacgagccc gtctcgatct gcttggcgga ggcgaagagc gtgacgttcg
     2221 cggtgtccgg atagatcacg ccggagatcg tcgtgccgct ctgatccttg aggatcgttc
     2281 ccgtggtggt cccctgaagc atcccctcgc tcacgctgag cgtgacgtcg atcttcttcg
     2341 aggtcgtcac gaggttgtcc gactggtcgc gcagctcgac ggcgaccgag aagggctggc
     2401 cggcgatggg caccgggagg ccggtcccct ggtccgtgac cgcggtgatg acgagcttcg
     2461 tggatcccgg aggggcgccc atcgccgtcc cgccggccac gacaccggtc acgaccaccg
     2521 ccgtggccag cgcgacccag gcgcgctgga gcccgtgcat cctgctggat ccgatccgaa
     2581 gcatctccca tccctcccgt cgccgtgccc gtgacctgtc cgcgggtctc cccatacgag
     2641 tgtgggcggg atgggggtcc tgcgaaggtc ctaccctagc acgcgaggcc gtgagggggg
     2701 aaggccctcc cgctatgatg ccggggtgct cgacgccgag ctgggcctcg ccaacgagct
     2761 gatcgaccgc gccgcgtcga tcgcgatgcc gctcttccgc cgcggccccg aggttcgcgt
     2821 gaaggacgac gccacgcccg tcacccaggc ggatctcgag atcgaggaga tggtccggga
     2881 gcagatcggg aagcggttcc cgtcggacgc ggtgctgggc gaggagcgcg ggggctcgat
     2941 ccgggacggg ccccgggtgt gggtcgtgga cccgatcgac ggcacgaaga acttcgcggc
     3001 ggggatccag gtctggggga ccctgctcgc gctgttcgag gggggcgagc cggtgctcgg
     3061 ggtcgtgggg gcgcccgccc tcggcgagcg gtacgaggcg gtccgagggc agggggcgac
     3121 gctcaacgga accctgatca gggcgtcgga cgtcgggtcc ctggaccccg ccgccgtctg
     3181 ccactacgcg accgaggcct ggctggaggg cccgtacgag gcggcgctgc gcgacctcga
     3241 ccgagccgcg taccgctccg tgggcttcac ggacttctgg gggcactgcc tcgtggcccg
     3301 cggctcggtc gaggtcgcgc tcgagcccga gctgcggatc tgggactggg gggccctcaa
     3361 ggtgctcgtc gaggaggcgg gcgggcggat gaccacgttc cagggggagc cggtgagcga
     3421 ccgaagctcg gtgctctcca cgaacgggat gctgcaccag acggtgctcg agatcctgac
     3481 gaccgactga gggcttccgc gtcggtcccg ggtggtaggt tgtccgcgtg agcgactccc
     3541 cccgcttcca tccgctggtc gaaggctggt tcgccgagac cctgggcgag cccaccgcca
     3601 tgcaggccga gggctggcga cgcatcgccg caggccgcga cactttggtc gctgcgccga
     3661 ccggctcggg gaagacgctc gccgccttcc tctgggcgat cgacgagctc gtgcggcggg
     3721 ccgaggcggg gacgctcgag gacgagacct ccgtcgtcta cgtctcgccg ctgaaggcgc
     3781 tgggcaacga catccagagg aacctccggg acccactgga ggcgatccgg gagcgggccg
     3841 ccgagcgagg gctcgagctt cccgagatcc gggtcctcgt gcggtcgggc gacacgcccc
     3901 agtctgagcg gcaggcgatg atcaggaagc ccccgcacat cctggtcacc accccggagt
     3961 cgctcttcat catgctcacg gccgagcgct cccgcgggtt cctgcggtcg gcccgcaccg
     4021 tgatcgtgga cgagatccac gccgtggccg gcgacaagcg cggcgcccac ctggcgctct
     4081 cgctggagcg gttggatcgg ctggccgagc agcccctcca gcggatcggg ctttcggcca
     4141 cccagaagcc gatcgaggag gtcgggcggc tcctcgtcgg gaacgggcgc ctgcgcgacg
     4201 acggttctcc cgactgcgag atcgtggacg ccggccaccg ccgcgagtgg gacctctccg
     4261 tggagatctc cgacgcgccg ctcggtccca tcgcgacaca cgagctccgg gccgagatgt
     4321 acgaccggat cgcggagctt tcgaagcagc accgcacgac gctcgtcttc gtggacacgc
     4381 ggcgcctcgc ggagcgcgtc acgcacgccc tggaggagcg gctcggcgag gagcacgtcg
     4441 cgccccacca cggctcgctc tcgcgcgaga tccgcctgcg ggcggagcgg gggctgaagg
     4501 aggggtcgct gcgcgtggtc gtggccaccg cctcgctcga gctcgggatc gacatcgggc
     4561 acgtggacct cgtctgccac gtcggcgccc cgcgctcgat cgccaccctg ctccagcgga
     4621 tcgggcggtc gggccacttc ctgggcggca ccccgaaggg cgccatcttc cccatgaccc
     4681 gcgacgagct gctccagacg gcggcggcga tccgcgccgc gcgggccggt gagctcgacc
     4741 gcgtgatctt cccccgcgag ccgctcgacg tcctcgccca gcagatcgtg gcgatcgccg
     4801 ccgccgagga tgtgggggag gacgagctct tcgccctcgc ccgccgggcg ttccactacc
     4861 gggacctctc gcgcgaggac ttcgacgagg tggtcgagat gctgtccgag ggcgtcgccg
     4921 gccggcacgg ccgacgctcg gcgcacctcc accgcgaccg ggtcaacagc gtgctcaagg
     4981 gccgccgcgg ggcccggctg gcggccatca cgtccggcgg cgcgatcccc gacatcggcg
     5041 actacgacgt ggtcgaggag ccggccggca ccttcgtggg caccgtgaac gaggacttcg
     5101 ccatcgagtc gatggccggc gacatcttcc tcctcggcaa cacctcgtgg cggatccgcc
     5161 gggtggaggc ggggaaggtc cgggtggaga acgcccacgg gctccccccc acgatcccgt
     5221 tctggatcgg cgaggctccg gggaggaccg aggagctctc gaccgcggtc tcggacctgc
     5281 gcgccgaggt ggcctcacgc ctggaggacc cggaggcggc ggcccgatgg ctggtcgagg
     5341 agaccgggat tcccgaggag ggcgccgagc agatcgtgga gcacctctct gaggggctgg
     5401 cgctgctcgg cgcgatcccc acgaaggaca ccgtcgtggc cgagcggttc ttcgacgagt
     5461 cgggcgggat gcagctgatc atccacgcgc cgttcggagc gcgcatcaac cgagcctggg
     5521 ggctctcgct ccgcaagaag ttctgcgtac agttcgactt cgagctgcag gcggccgcca
     5581 ccgacgacgc gatcctgctc tcgctcgggg agcagcacag cttcccgctg gacagcgtgg
     5641 ccgggtacct caagcctccg acccttcgga acacgctgat ccaggcgatg ctcccctccc
     5701 ccatgttcgc caaccggtgg cgctggaacg cgacgcgggc gctggccctc ctgcgccaca
     5761 gcggcggccg caaggtgccg gtcccgatcc agcggatgcg ggccgaggac ctgctgtcgg
     5821 cggtcttccc cgagcaggtg atgtgccagg acaatcggat gggcgatatc gacccgcccg
     5881 accatccgat cgtgaacgag accgtgaagg actgcctgca cgaggcgatg gacctcgacg
     5941 gactggccga gatcgtggag cggatcggtg gaggcgagat ccggttcgtg gtggcggaga
     6001 cgccggcgcc ctcggtcttc tcgcacgagg tgctgaacgc ggggccgttc gccttcctgg
     6061 acgacgcgcc gctcgaggag cggagggcga gggccgtctc ccttcgccgc gtggaccccg
     6121 acctccagga cgggctcggc gcgctcgacg ccgaggcggt ggcggccgtg cgcgaggagg
     6181 ccatgcccga ggtgcgcgac gccgacgagc tgcacgacct cctgctctcg ctgggggtgc
     6241 tgccgctgga ggcggggtgg gtcgagatgg ccgaggagct ccgcgcggcc ggacgggcga
     6301 ccgtggctca ctggaacgga cgcagcgcgt ggctcgcggc ggagcggctg gccaaggtgc
     6361 gggccgcgat cccagatctc cgcttggagg acgacctgcc cctggtgggc gacgagtcgc
     6421 cgtcccacga cgaggccgtc cgggacgtcg tgcacgggtg gatgctggtg gcggggccgg
     6481 tgacggccgc ggcgttgtcc gagcggatcg ggctgcccga gcccgacgtc gatcgcgccc
     6541 tgctgggcct cgaagcgtcc gggatcgtcc tgcgcggccg cttcacgggg gcgcccgagc
     6601 tcgagtggtg cgagcggcgc ctgctctcac ggatccaccg ccggacgata cggcgcctgc
     6661 gagccgagat cgagccggtg gaccccgcgg cgctgatgcg gttcctcctc cggtggcagc
     6721 acattcagcc cgggacccag ctgcacgggc gggaggggat gctgcgggtc ctggagcagc
     6781 tccagggctt cgagctcccc gcgcccg
//
LOCUS       DASUTW010000062         4166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1054217, whole genome shotgun sequence.
ACCESSION   DASUTW010000062 DASUTW010000000
VERSION     DASUTW010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054217"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..426)
                     /locus_tag="VF058_02200"
     CDS             complement(34..426)
                     /locus_tag="VF058_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4260 domain-containing protein"
                     /protein_id="HEX6207147.1"
                     /translation="MTRPEAPSVVGLPKVLLRSEGLTVLALAILLYAKVGRSWWLFAI
                     LLLAPDLGMLGYVRGTRVGAVAYNAFHTYVAAGTLALVGVIAEHELTIALALIWFAHI
                     GFDRALGYGLKYPEGFGSTHLGRIGRRG"
     gene            complement(423..1778)
                     /locus_tag="VF058_02205"
     CDS             complement(423..1778)
                     /locus_tag="VF058_02205"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6207148.1"
                     /translation="MGGTASKAEAAGDHQATRSAGRPTGAVMGRFTLRFQDPYLEAAY
                     RAAHYRQNIVNIRVAHVLGALLMVAWGFAVHGHLPFWDRPFDLAIRYGVLIPILLIGF
                     VLTFMRWYPRFWEWELLGTLGLIMPLWVYYVSKLLVMPPDFGYVGLLLMAMFVYTLIR
                     PRFPLVLVIAALPILLYVPYAVVAVQVSGVKTVIAIFYLVSFGGLGVVAARRSEQNIR
                     LLFLRERQLDRERERSDSLLLNILPKVIVARLKREPEGGRLAEGFDEVSVLFADAVAF
                     TEQAAKTSPEDLVEALDELFRRFDELSDAYGLEKIKTVGDAYMAVAGAPVPNPDHAEA
                     AAEMALGILEESSRVRWPSGDPIVMRVGVATGPAVAGVIGQRKFAYDLWGDTVNLASR
                     LEEHGEPGCILISEELAERLGDRYRLGPPKVMDLKGKGPTPVRFLMGRVDAKVPSPAD
                     A"
     gene            complement(1863..2765)
                     /locus_tag="VF058_02210"
     CDS             complement(1863..2765)
                     /locus_tag="VF058_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013476669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX6207149.1"
                     /translation="MLRRALALAFTALLVAAPAAAQPFPDVIPLVDGSQPEGIATGRG
                     TTFYVGSIPTGSVYRGDLRTGEIELLVDRDDRAAIGMAVDHRSRLFVAGGPTGVAFVY
                     DARTGAEIATYDLADGETFINDVVVTREAAWLTDSFNPFLYRIPIGPGGRLAAGAEQV
                     PLGGDYQHLPGFNLNGIEATPDGRWLIAVHAAIGDLYRIDPATGEATIIDLGGDAVPN
                     GDGILLDGKRLYVVQNFFNQIAVVDLAPDLASGEIVDVITDSRFDIPTTIAEHGHSLY
                     AVNARFSTPPTPETEYDVVRVEKV"
     gene            2848..3768
                     /locus_tag="VF058_02215"
     CDS             2848..3768
                     /locus_tag="VF058_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018353004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX6207150.1"
                     /translation="MSPREERIALASFIAGSVLAGGNAVAVRFSNRELEPLWGAGLRF
                     ALAGLLGLGVMAAMRLAFPRGRALWWAMLYGTLNFAGSFSLAYYALLELHAGFGQTLL
                     SLVPLATLLVAVAWRQERLRASGVVGSLVALAGVAVMSGASVQEVHLPSVLASVGAAL
                     CFAQAAVLVRWMPRVHPVTMNAVGMATGAVLLILGSLIAGEAIELPQRAATWWSIAYV
                     VPIGSIVVFFLYLVVLRYWRASRAAYEFVIIPFFAVALSAWLDDEPIGWGLVLGGLLV
                     LAGVYVGALRPGIERDESAPAEPEVSSDLR"
     gene            3802..4137
                     /locus_tag="VF058_02220"
     CDS             3802..4137
                     /locus_tag="VF058_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207151.1"
                     /translation="MTSLARRTLPVLVLALVLVPVLGGAASAKVEKFTFTDVLLVDGD
                     GTEISGAVIEANVKQTNKRTTGYCGYFTEGYQESLGYYFNDFTVDPDQELGDQLLRFC
                     QRWFPDRFV"
BASE COUNT          618 a         1433 c         1461 g          654 t
ORIGIN      
        1 cgcgagagac gtcgttcgag cacggcaccc atcctatccg cggcggccga tgcggcccag
       61 gtgcgtgctt ccgaacccct ccgggtactt cagcccgtac ccgagcgcgc ggtcgaaccc
      121 gatgtgcgcg aaccagatca gggccagggc gatcgtcagc tcgtgctcgg cgatcacccc
      181 caccagggcg agggtcccgg ccgcgacgta ggtgtggaag gcgttgtacg cgacggcgcc
      241 cacccgggtc ccgcggacgt agcccagcat cccgagatcg ggggcgagca gcaggatggc
      301 gaacagccac caggagcgac ccaccttcgc gtagagcaaa atcgcgagcg cgagcacggt
      361 cagcccctcc gagcgaagga gcacctttgg gagcccgacg accgacggcg cctccggccg
      421 cgtcacgcgt ccgcgggcga ggggaccttc gcatccaccc gtcccatcag gaaccggacc
      481 ggcgtgggac ccttcccctt gagatccatc accttgggcg gccccaatcg gtagcgatcc
      541 cccagccgct ccgccagctc ctcggagatc aggatgcagc ctggctcgcc gtgctcctcg
      601 aggcggctgg ccaggttcac cgtgtcgccc cagaggtcgt aggcgaactt ccgctgcccg
      661 atcaccccgg ccacggccgg gccggtggcc acgccgacgc gcatcacgat cgggtcgccc
      721 gagggccacc ggacgcgcga ggactcctcg aggatcccga gcgccatctc agccgccgcc
      781 tcggcgtggt ccgggttcgg gaccggcgcg ccggccaccg ccatgtacgc gtcccccacg
      841 gtcttgatct tctccagacc gtacgcgtcg gacagctcgt cgaacctccg gaacagctcg
      901 tcgagcgcct cgaccagatc ctccggcgac gtcttcgcgg cctgctcggt gaacgccacg
      961 gcgtccgcga acaggacgct cacctcgtcg aagccctcgg cgagcctgcc cccttccggc
     1021 tcccgcttca gcctggccac gatgaccttg gggaggatgt tcagcagcag cgagtccgag
     1081 cgctcgcgct cgcgatcgag ctgccgctcc cggaggaaca ggaggcggat gttctgctcg
     1141 ctccgtcgcg cggcgaccac acccaggccc ccgaacgaca cgaggtagaa gatggcgatg
     1201 accgtcttca cgcccgacac ctggaccgcg acgaccgcgt acgggacgta caacaggatc
     1261 gggagcgcgg ctatcacgag gacgagcggg aaccgaggcc ggatcagcgt gtagacgaac
     1321 atcgccatca gcaggagccc cacgtacccg aagtcgggcg gcatcacgag gagcttcgag
     1381 acgtagtaga cccagagcgg catgatgaga ccgagcgtcc ccagcagctc ccactcccag
     1441 aagcgcgggt accaccgcat gaacgtcagc acgaacccga tcaggaggat cgggatcaac
     1501 acgccgtacc tgatggcgag atcgaagggg cggtcccaga acggcaggtg cccgtggacc
     1561 gcgaaccccc acgccaccat gagtagggcc ccgaggacgt gcgccaccct tatgttgacg
     1621 atgttctgcc ggtagtgggc ggcgcggtac gctgcctcga ggtaggggtc ctggaagcgc
     1681 agggtgaagc gtcccatgac cgcccccgtc gggcggcccg cggaccgggt cgcctggtgg
     1741 tcgccggccg cctcagcctt cgacgcggtg cctcccatga cctccatcat cctctctcgg
     1801 gagggcagcg tcgaggaaac gccggatctc gcggacgaac ctgttcgtga ggcaggccgg
     1861 gctcagacct tctccaccct caccacgtcg tactcggtct cgggggtcgg cggcgtcgag
     1921 aagcgcgcgt tgaccgcata cagcgagtgg ccgtgctccg cgatcgtcgt cgggatgtcg
     1981 aacctcgagt ccgtgatgac gtccacgatc tcgccgctcg cgaggtcggg cgcgaggtcg
     2041 accaccgcga tctggttgaa gaagttctgc acgacgtaga ggcgcttccc gtcgagcagg
     2101 atcccatcgc cgttcggaac ggcgtcgccc ccgaggtcga tgatcgtggc ctcgcccgtg
     2161 gcggggtcga tccggtagag gtcgccgatc gcggcgtgca ccgcgatcag ccaccggccg
     2221 tccggcgtgg cctcgatccc gttgaggttg aagcccggga ggtgctggta gtcgccgccc
     2281 agcggcacct gctcggcgcc cgcggccagg cggccgccgg ggccgatggg gatccggtac
     2341 aggaacgggt tgaacgagtc cgtgagccag gccgcctcgc gcgtgacgac cacgtcgttg
     2401 atgaacgtct cgccgtccgc gaggtcgtag gtcgcgatct cggctcccgt gcgggcgtcg
     2461 tagacgaagg cgacgccggt cggcccaccg gccacgaaca gcctgctgcg gtggtcgacg
     2521 gccatcccaa tcgcggctcg gtcgtcccgg tccacgagca gctcgatctc gccggtgcgg
     2581 agatcgcccc ggtagacgga tccggtcggg atcgatccca cgtagaaggt cgttcctcgc
     2641 ccggtcgcga tcccctcggg ctgggagccg tcgacgagcg ggatcacgtc ggggaagggc
     2701 tgggccgccg cgggggccgc gacgagcagg gcggtgaacg cgagtgcgag cgcgcgacga
     2761 agcattgggg tcacctccgg atgatcgtgc gtcgggcttc catgctatcg ggtgcgcgag
     2821 ataacgcacc gcgtaggatg gctctcgatg tccccacgag aggaacgcat cgcgctcgcc
     2881 tccttcatcg ccggctcggt gctggccgga ggcaacgccg tggccgtgcg cttcagcaac
     2941 cgcgagctgg agccgctgtg gggggcgggc ctgcggttcg cgctggccgg cctgctgggc
     3001 ctcggcgtca tggccgcgat gcggctcgcc ttccctcgag gtagggcgct gtggtgggcc
     3061 atgctctacg ggacgctcaa tttcgccggc tcgttctcgc tcgcctacta cgcgctcctg
     3121 gagctccacg cggggttcgg ccagaccctg ctctcgctcg tccccctcgc cacgctgctc
     3181 gtggccgtcg cgtggcgcca ggagcggctc cgggcgtcgg gcgtggtggg ctccctggtc
     3241 gcgctggcgg gggtggcggt gatgtcgggc gcctccgtgc aggaggtgca cctgccgtcg
     3301 gtgctcgctt cggtgggggc ggccctctgc ttcgctcagg cggcggtgct cgtgcggtgg
     3361 atgccccgcg tccacccggt gacgatgaac gcggtgggga tggccaccgg cgccgtgctc
     3421 ctcatcctgg ggtcgctgat cgcgggcgag gcgatcgagc tgccacagcg cgcggcgacg
     3481 tggtggtcga tcgcgtacgt cgtgccgatc ggatcgatcg tcgtcttctt cctctacctc
     3541 gtcgtgctcc gctactggcg ggcgtcgagg gccgcgtacg agttcgtcat catcccgttc
     3601 ttcgcggtgg cgctctcggc ctggctggac gacgagccga tcggctgggg gctcgtgctc
     3661 ggtgggctcc tggtgctggc tggggtgtac gtcggagcgc tgcgcccggg catcgagcgc
     3721 gacgagtctg ctccggcgga gcctgaagtt tcgagcgacc tgcgataggt tgagccgcgt
     3781 ccccgagatg aggaggtcgc catgacatcc ttggctcgac gcacactgcc cgtgctcgtc
     3841 ctggctctcg tcctggtacc cgtcctgggc ggcgccgcat cggcgaaggt cgagaagttc
     3901 accttcaccg acgtcttgct ggtcgatggc gacggcaccg agatcagcgg cgcggtgatc
     3961 gaggccaacg tgaagcagac gaacaagcgc acgacggggt actgcgggta cttcaccgag
     4021 ggctaccagg agtcgctcgg gtactacttc aacgacttca cggtggatcc ggatcaggag
     4081 ctgggggatc agctgctcag gttctgccag cgctggttcc cggaccggtt cgtctgagcc
     4141 tcagcggaac cttcagcggc gggggg
//
LOCUS       DASUTW010000063         2677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_667078, whole genome shotgun sequence.
ACCESSION   DASUTW010000063 DASUTW010000000
VERSION     DASUTW010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667078"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VF058_02225"
     CDS             <1..547
                     /locus_tag="VF058_02225"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6207152.1"
                     /translation="SELPGITGSFLRVHFGIILALLAAWGVWWLLFRTTKGFEFRAVG
                     SNPNAARYAGMSVGLTYVLAMSLAGSLAGLGGASQVLGLQKFLTPGFASGIGFTAIAL
                     ALLGKAHPLGVVAASLLFGMLQAGAVSMQAATRTPVDIIVVIQALIIVFIAAPALVRA
                     IYRISVGREVGAEVFTKGWSG"
     gene            544..1794
                     /locus_tag="VF058_02230"
     CDS             544..1794
                     /locus_tag="VF058_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6207153.1"
                     /translation="MSATAATVSGRVSVRRARVLGVLFLLMAAFTAGFFGPRAPGGET
                     ASFFLGANAPDLSLPVRATLYALSALMAFLGAVQLVRGFGARTYVILGIVLGVFIFSL
                     LVYAARGATLNLTAMLNSTLLRSVPITLGALSGVMCERAGVINIGIEGIFLAAAFTGA
                     FLGSVSGSLWVGLISGVLVGGLLGWVLAVLSIRYRVDQIVAGTVINIFALGITSFLSA
                     SILQENQALNNPGRFQVFEIPLLSEIPVAGPVLFRNNVFVYLMFGLLFLVHIGLFRTR
                     WGLRVRAVGEHPLAADTVGIRVLFERYRSVILGGMMAGLGGAFFTLGSVGRFDEGMTA
                     GRGFIALAAMIFGRWSPWGAFGAALVFGFADSLQTRLAILEVGLPSEFLLMAPYIATI
                     LVVAGLVGRARPPAADGKPYIKEG"
     gene            1988..>2677
                     /locus_tag="VF058_02235"
     CDS             1988..>2677
                     /locus_tag="VF058_02235"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017565083.1"
                     /note="catalyzes the formation of
                     3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate from
                     phosphoenolpyruvate and D-erythrose 4-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX6207154.1"
                     /translation="MVVVMKMGASDAEVEGVVQKVQESGGEAFVSRGRFWTIIGLVGD
                     TTGFLELPLESLPGVDRVVRVGRPYKMVARELHPEPSTVRIGGVPIGRQSVTVIAGPC
                     AVESEEQALASAEMARRAGAHLLRGGAYKPRTSPYAFQGLRERGLEILGVCREATGLP
                     VVTEVLEARDVERVAEVADGLQVGARNMHHFPLLQEVGQTGKPIVLKRGLSATVEEWL
                     MAAEYVAQQGNS"
BASE COUNT          321 a          890 c          994 g          472 t
ORIGIN      
        1 atcggagctg ccgggcatca cgggctcctt cctccgcgtc cacttcggca tcatcctggc
       61 cctgctggcg gcctggggcg tctggtggct gctcttccgg accaccaagg gcttcgagtt
      121 ccgagccgtc ggctcgaacc ctaacgcggc ccggtacgcg gggatgagcg tggggctgac
      181 ctacgtcctg gcgatgagcc tggccgggtc cctggccggg ctcgggggcg catcgcaggt
      241 gctcgggctc cagaagttcc tgacgcccgg cttcgccagc ggcatcgggt tcacggccat
      301 cgccctggcg ctgctgggca aggcgcatcc gctcggcgtc gtcgcggcct cactgctgtt
      361 cgggatgctc caggccggcg cggtctcgat gcaggccgcc acccgcacgc cggtggacat
      421 catcgtggtg atccaggccc tgatcatcgt cttcatcgcg gcgcccgcgc tcgtccgggc
      481 gatctaccgg atcagcgtgg gccgtgaggt gggggccgag gtcttcacga aggggtggag
      541 cggatgagcg cgacggccgc caccgtctcg ggtcgggtct ccgtccgaag agctcgcgtg
      601 ctgggggtcc tgttcctcct gatggcggcg ttcacggcgg ggttcttcgg tcccagggcc
      661 cccgggggag agaccgcctc cttcttcctg ggggcgaacg ccccggacct ctccctcccg
      721 gtccgggcca ccctgtacgc gctctcggcc ctgatggcct tcctgggggc cgtccagctc
      781 gtgcgcggct tcggcgctcg gacgtacgtg atcctgggga tcgtcctcgg ggtcttcatc
      841 ttctcgctcc tggtgtacgc ggcccgcggc gccaccctga acctcaccgc gatgctcaac
      901 tccacgctgc tccgctcggt ccccatcacc ctcggggcgc tgtcgggcgt catgtgcgag
      961 cgggccggcg tcatcaacat cgggatcgag gggatcttcc tggcggcggc gttcaccggg
     1021 gccttcctgg gaagcgtctc ggggagcctc tgggtgggac tgatctcggg cgtcctcgtc
     1081 ggggggctcc tgggctgggt gctggccgtc ctctcgatcc gctaccgcgt ggaccagatc
     1141 gtcgcgggga ccgtcatcaa catcttcgcg ctggggatca cgtcgttcct ctcggccagc
     1201 atcctccagg agaaccaggc cctgaacaac ccgggacggt tccaggtgtt cgagatcccc
     1261 ctgctctcgg agatccccgt ggccgggccg gtcctgttcc ggaacaacgt cttcgtgtac
     1321 ctgatgttcg ggctgctgtt cctggtgcac atcgggctgt tccgcacgag gtggggcctg
     1381 agggtgcggg ctgtcggcga gcacccgctg gcggccgaca cggtggggat ccgcgtgctg
     1441 ttcgagcgct accgaagcgt gatcctgggc gggatgatgg cgggcctggg cggcgccttc
     1501 ttcacgctcg gctccgtggg acgctttgac gaggggatga cggcgggccg ggggttcatc
     1561 gccctggccg cgatgatctt cgggcgctgg tcgccgtggg gcgcgttcgg cgcggcgctg
     1621 gtgttcgggt tcgccgactc cctccagacg cgactggcga tcctggaggt cgggctcccc
     1681 tccgagttcc tcctgatggc cccctacatc gccacgatcc tcgtcgtggc ggggctcgtg
     1741 gggagagcca ggcccccggc ggccgacggg aagccgtaca tcaaggaggg ctgacccctc
     1801 cggggttgtg cgatccgcgc actctctcca caatggcagc gacatgagga acggtcggca
     1861 cggcttcgcg tggtatcgga gcggcggagg gcgctgctcg gactgacctg cggagcgacc
     1921 ggcaaccagg accccgggca gcggcccggg gttccgtgtt tcccgggccc tctcacggag
     1981 gagcgccatg gtcgtcgtga tgaagatggg agcgagcgac gccgaggtcg agggcgtcgt
     2041 gcagaaggtc caggagtcgg gcggcgaggc cttcgtgtcg cgcggccgct tctggacgat
     2101 catcgggctg gtcggcgaca cgacggggtt cctcgagctg ccgctcgagt cccttccggg
     2161 ggtggaccgc gtcgtcaggg tgggccgccc gtacaagatg gtggcgcggg agctccaccc
     2221 tgaaccctcc acggttcgga tcggcggggt gccgatcggg aggcagtcgg tgaccgtcat
     2281 cgcgggtccc tgcgcggtgg agtccgagga gcaggcgctg gcgtcggctg agatggcccg
     2341 gcgggccggc gcccatctcc tccgcggtgg ggcgtacaag ccgaggacgt ccccctacgc
     2401 cttccagggg ctgcgtgagc ggggcctgga gatcctcggt gtttgccgcg aggcaaccgg
     2461 gctccccgtg gtcaccgagg tgctcgaggc ccgcgacgtg gagagggttg cggaggtggc
     2521 cgacggcctc caggttggcg cccggaacat gcatcacttc ccgctgctcc aggaggtggg
     2581 acagacgggc aagccgatcg tcctgaagcg cggcctgtcg gcgacggtcg aggagtggct
     2641 gatggccgcg gagtacgtcg cccagcaggg gaacagc
//
LOCUS       DASUTW010000064         6346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_278628, whole genome shotgun sequence.
ACCESSION   DASUTW010000064 DASUTW010000000
VERSION     DASUTW010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6346
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278628"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1041
                     /locus_tag="VF058_02240"
     CDS             94..1041
                     /locus_tag="VF058_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX6207155.1"
                     /translation="MYRTMIMARLCDEAQFRLNRQGKAPFVVPVSGHEAVQIGTAWPM
                     VKGKDIFVPYYRDLGICLVAGMTPKDVFLGVFGKADDPSSGGRQMPAHWGSRRLGIIT
                     GSSPIATQIPHASGIAYAMKYRGEDSVVASWFGDGATSEGDWHEALNFAGIHRLPVVF
                     VCENNQYAISVPLSKQMAVKDVAVRAEAYGFEGVVVDGNDALACYEAMSAAVDKARAG
                     GGPTLIEAKTYRYHPHTSDDDDRTYRTREEVEEWKKKDPLILFGEYLKDHGVLSGDDE
                     IERLRAETKEEVDRNVEEAWEAPDPEPESAMRHVWAEEA"
     gene            1043..2029
                     /locus_tag="VF058_02245"
     CDS             1043..2029
                     /locus_tag="VF058_02245"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017755705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX6207156.1"
                     /translation="MAEKNIVQTIHDTMFEEMKADDRVILLGEDVGARGGVFRASANF
                     LETFGEERVLDTPLAESSIVGVAIGMALHGLLPIAEIQFADFIHPAYDQIVSEAARIR
                     YRSNGDFGVPLVIRAPYGGGVHGALYHSQSIEATYGHVPGLKVVLPATPHDVKGMLRA
                     SLRDPDPVLFLEHKKTYRLIKGEVPDEDYEVPIGKADVKREGEDMTVIAYGLMLHHAL
                     EAAQKLQDEDGASVEVIDVRTISPLDKETILSSVGKTAKAMVVYEDNRTYGVGAEIVA
                     TIAEEAMFDLDGPLVRIGGPDIPAMPFSGPLEKFFMPNVEGIHQAMKELAEF"
     gene            2060..2506
                     /locus_tag="VF058_02250"
     CDS             2060..2506
                     /locus_tag="VF058_02250"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6207157.1"
                     /translation="MRIEASTTIDRPLADVWRWYAVEHVINHPRWDPDMELEQITEGP
                     MALGTRIRRRNVRWGEPVEGEMEVVEWAPERSVGFHIRDANMEMRGRAAFQDDGPGRT
                     LLTVATDVPELDESKRGFLSSMMERSLSNVKRLAESDIERASRAGG"
     gene            2526..3512
                     /gene="lipA"
                     /locus_tag="VF058_02255"
     CDS             2526..3512
                     /gene="lipA"
                     /locus_tag="VF058_02255"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX6207158.1"
                     /translation="MRFRDPSRDKNPRRKRLHLPIMEAEAADTHVAHSVGCAQQVVPQ
                     RKPPWMRAHLRTGPGYRELKGLMEGLSLNTVCEEAGCPNIFECWEQREATFLILGNTC
                     TRRCGFCDIQTGKPSAVDEEEPARVAEAVASMGLRHVVLTGVARDDLPDGGASIWAGS
                     IRAVREAVPSCGIEVLPPDFKAHREQIGIVITEEPDVFAHNLETVRRLHDRIRPGFDY
                     DRTLDALRLAKELRPGQVTKSNLILGMGEGPGEVEGALRDLRDAGVDLMTIGQYLQPT
                     AYHLPVDRWVTPEEFEEHARMGRELGFAHVEAGPLVRSSYRAGKQLAAARRS"
     gene            3526..4617
                     /locus_tag="VF058_02260"
     CDS             3526..4617
                     /locus_tag="VF058_02260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01186.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX6207159.1"
                     /translation="MISLRDVSKVYDGAAPAVDELSLEIDEGEIVVLVGPSGCGKTTT
                     LKMINRLIEPTSGEILVNGRNVLEQDPVELRRGIGYVIQSIGLLPHRTIRQNIATVPD
                     LLRWERPRTRARVDELAEMLRLDHGLLDRYPGELSGGQRQRVGVARGLAADPPVMLMD
                     EPFGAVDPIIRERLQDQFLEIQRELKKTIVFVTHDVDEAIKMADRIAILNIGAVVEQY
                     DAPEAILSEPASDFVKSFVGLERGLKRLSLIKVADIEVEEGPIVAPTDDVSTALAEME
                     RSGFGWASVVEEGRLLGWVDPGCLEGMASVGQYPPRRFSATVTDGSSLREALDTIVTS
                     RTNVAVVVTNGGRYRGVLTLEKLTRELIA"
     gene            4614..5288
                     /locus_tag="VF058_02265"
     CDS             4614..5288
                     /locus_tag="VF058_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6207160.1"
                     /translation="MTGRPFWDWDWVGRNGDLILDQAVQHLWLTFLAVVIGLAISFPL
                     AVLAYRRRRLYPPIAWLTGTLYTIPSLALFAFLTPFTGLSTLTAEIGLVSYTLLILIR
                     NIVTGLRAVPPEVREAAVGMGYTDRQLLWRIELPLALPAVVAGLRIATVTTIGLVTVT
                     ALIGQGGLGALMLGGFRRLYPELAVVLGVGLSVALAVAIDLLLFAGQRWITPWSRRAG
                     DRVVAT"
     gene            5237..6019
                     /locus_tag="VF058_02270"
     CDS             5237..6019
                     /locus_tag="VF058_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020572482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6207161.1"
                     /translation="MDHALEPAGRGSSGRDVTEPGFFSQVVDWFADAANWSGDFGIPS
                     RLLEHVVMSGVSVAIATALALPLAMYIGHRRRLERLVVSITNLGRAIPSFGLLFLFVL
                     WLGVGLSSPPALRPAIILALVLLAIPPILTNAYVGIQSVDDDTLEAARGMGMSEWSIL
                     FRVEVPLGMPLIVAGLRTAAVQVVATATLGAVVAGGGLGRYIIDGFAVRDLPQIFAGA
                     ILVAALALAVDVLIGGVGRLLSPRVATRPGAATERGITAGAA"
     gene            6089..>6346
                     /locus_tag="VF058_02275"
     CDS             6089..>6346
                     /locus_tag="VF058_02275"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX6207162.1"
                     /translation="MRRTFRTPVLGAALVALALIAAACDGSGEDGDGDGESRGQITVG
                     AVAFAENQILGEMYAQALEDAGYEVSRQLSLDSREILQPAIE"
BASE COUNT          993 a         2157 c         2261 g          935 t
ORIGIN      
        1 cgggtaaccg caaggaggtt gttccgcgtg gcgacgaaga cccaggaaga ccgccattcc
       61 gagctcggcc tctccgcaga cgacctgcag gccatgtacc ggacgatgat catggcccgc
      121 ctgtgcgacg aggcgcagtt caggctcaac cgtcagggca aggcgccgtt cgtcgtcccc
      181 gtctcggggc acgaggccgt ccagatcggg accgcctggc ccatggtcaa ggggaaggac
      241 atcttcgtgc cctactaccg cgacctcggc atctgcctcg tcgcggggat gaccccgaag
      301 gacgtcttcc tcggcgtctt cgggaaggct gacgacccgt cctcgggcgg gcgtcagatg
      361 ccggcccact ggggatcgag gcggctcggg atcatcaccg gctcctcccc catcgccacg
      421 caaatccccc acgcctcggg gatcgcctac gcgatgaagt accggggcga ggactccgtg
      481 gtcgcctcgt ggttcggcga cggcgccacc agcgaggggg actggcacga ggcgctgaac
      541 ttcgcgggga tccaccgcct gcccgtcgtc ttcgtctgcg agaacaacca gtacgcgatc
      601 tcggtgccgc tctccaagca gatggcggtc aaagacgtgg cggtacgcgc cgaggcctac
      661 ggcttcgagg gcgtggtggt ggacgggaac gacgccctcg cctgctacga ggccatgagc
      721 gcggcggtcg acaaggcccg cgccggcggc ggccccaccc tgatcgaggc caagacctac
      781 cgctaccatc cccacacctc cgacgacgac gaccggacct accggacgcg cgaagaggtc
      841 gaggagtgga agaagaagga ccccctgatc ctcttcgggg agtacctgaa ggaccatggg
      901 gtcctcagcg gcgacgacga gatcgagcgg ctccgcgcgg agacgaagga ggaggtcgac
      961 cgcaacgtcg aggaggcctg ggaggccccc gaccccgagc ccgagtcggc gatgcgccac
     1021 gtctgggcgg aggaggcctg agatggccga gaagaacatc gtccagacga tccacgacac
     1081 gatgttcgag gagatgaagg cggacgaccg cgtgatcctc ctcggcgagg acgtcggggc
     1141 ccgcggcggc gtgttccggg cctcggccaa cttcctggag acgttcggcg aggagcgggt
     1201 gctcgacacc ccgttggccg agtcctcgat cgtcggcgtg gcgatcggga tggcgctcca
     1261 cgggctgctg ccgatcgccg agatccagtt cgccgacttc atccatcccg cgtacgacca
     1321 gatcgtgtcg gaggccgcgc ggatccgcta ccggtcgaac ggggacttcg gggtgccgct
     1381 cgtcatccgc gccccctacg gaggcggggt ccacggcgcg ctctaccact cgcagtcgat
     1441 cgaggccacc tacgggcacg tccccgggct gaaggtcgtc ctgccggcca cgccgcacga
     1501 cgtgaagggg atgctgcgtg cctcgctccg ggaccccgac cccgtgctgt tcctggagca
     1561 caagaagacc taccggctga tcaagggcga ggtccccgac gaggactacg aggtcccgat
     1621 cgggaaggcc gacgtgaagc gcgagggcga ggacatgacg gtgatcgcct acggcctgat
     1681 gctccaccac gcgctcgagg ccgcccagaa gctccaggac gaggacggcg cctcggtcga
     1741 ggtgatcgac gtccgcacga tcagcccgct ggacaaggag acgatcctct cgtccgtggg
     1801 gaagacggcg aaggcgatgg tggtctacga ggacaatcgg acctacggcg tgggcgcgga
     1861 gatcgtggcc acgatcgccg aggaggcgat gttcgacctc gacgggccgc tcgtacgcat
     1921 cggcgggccg gacatccccg cgatgccgtt ctccggaccg ctcgagaagt tcttcatgcc
     1981 gaacgtcgag gggatccacc aggcgatgaa ggagctggcc gagttctgag cgtccgggcc
     2041 cgccgagcgg gaggatcgca tgcggatcga ggcatcgacg acgatcgacc gaccgctggc
     2101 cgacgtctgg cggtggtatg cggtggagca cgtgataaac catccccgct gggacccgga
     2161 catggagctc gagcagatca ccgaaggtcc gatggcgctg ggcacgagga tccggcgaag
     2221 gaacgtccgc tggggcgagc ccgtcgaggg cgagatggag gtcgtggagt gggcgccgga
     2281 gcggtcggtc gggttccaca tccgcgacgc gaacatggag atgcgcggac gagcagcgtt
     2341 ccaggacgac gggcccggca ggacgttgct gacggtcgcg accgacgttc ctgagctgga
     2401 cgagtccaag agagggttcc tctcgagcat gatggagcgg agcctctcga acgtgaagcg
     2461 cctcgccgag tcggacatcg agcgcgcctc tcgggcaggg ggatgagcgg atagactggc
     2521 ccccgatgcg gttccgcgac ccgagccgag acaagaatcc tcgccgcaaa cggctccacc
     2581 tcccgatcat ggaggcggag gcagccgaca cccacgtcgc gcactcggtg ggctgcgctc
     2641 agcaggtcgt cccgcagcgc aagccgccct ggatgcgggc ccacctccgg accggcccgg
     2701 gctaccgcga gctcaagggg ctcatggagg gcctctcgct caacaccgtg tgcgaggagg
     2761 ccggctgccc caacatcttc gagtgctggg agcagcgcga ggcgaccttc ctcatcctcg
     2821 gcaacacctg cacccgccgc tgcggcttct gcgacatcca gacggggaag ccctcggcgg
     2881 tcgacgagga ggagcccgcc cgcgtggccg aggccgtggc ctcgatgggc ctcaggcacg
     2941 tcgtgctcac cggcgtggcc cgcgacgacc tccccgacgg cggggcgagc atctgggccg
     3001 gctccatccg cgcggtccgc gaggcggtgc ccagctgcgg catcgaggtc ctcccgcccg
     3061 acttcaaggc ccaccgcgag cagatcggga tcgtgatcac cgaggagccg gacgtcttcg
     3121 cccacaacct cgagaccgtc cgccgcctcc acgaccggat ccgcccgggg ttcgactacg
     3181 accgcacgct cgacgccctc cggctggcca aggagctgcg tccgggccag gtcaccaagt
     3241 ccaacctgat cctggggatg ggcgagggcc ccggggaggt cgagggtgcg ctccgcgatc
     3301 tccgcgacgc gggcgtggac ctgatgacga tcggccagta cctccagccg acggcgtacc
     3361 acctccccgt ggatcgctgg gtgaccccgg aggagttcga ggagcacgcg aggatgggca
     3421 gggagctggg gttcgcccac gtggaggccg ggccgctcgt ccgctcctcc tatcgggccg
     3481 ggaagcaatt ggccgccgcc cggcgttcct gagaccgagg cctccatgat cagcctgcgc
     3541 gacgtgtcga aggtgtacga cggcgcggcc ccggccgtgg acgaactctc cctggagatc
     3601 gacgaagggg agatcgtcgt cctcgtcggc ccctcgggct gcggcaagac cacaacgctg
     3661 aagatgatca accggctgat cgagccgacg tccggcgaga tcctcgtgaa cgggcgcaac
     3721 gtcctcgagc aggacccggt ggagctgcgg cgagggatcg gctacgtgat ccagtcgatc
     3781 gggctcctcc cccatcgaac gatccgccag aacatcgcca cggtgccgga cctgctccgg
     3841 tgggagcggc cccgaacccg ggccagggtc gacgagctcg cggagatgct ccgcctcgac
     3901 cacggcctgc tcgaccgcta cccgggcgag ctctccggcg gccagcgcca gcgggtcggc
     3961 gtagcccggg gtctggccgc agaccccccc gtcatgctga tggacgagcc cttcggcgcc
     4021 gtggacccca tcatccgcga gcgactccag gaccagttcc tggagatcca gcgcgaactg
     4081 aagaagacca tcgtgttcgt cacccacgac gtggacgagg cgatcaagat ggccgaccgc
     4141 atcgccatcc tgaacatcgg ggcggtggtc gagcagtacg acgcccccga ggcgatcctg
     4201 tccgagccgg ccagcgactt cgtgaagagc tttgtgggcc tggagcgagg gctgaagcgg
     4261 ctctcgctga tcaaggtggc cgatatcgag gtggaggagg ggccgatcgt cgcccccacc
     4321 gacgacgtgt ccaccgcact ggccgagatg gagcgttcgg gcttcgggtg ggcctccgtg
     4381 gtcgaggagg ggcgactgct gggctgggtg gaccccggat gcctcgaggg gatggcctcg
     4441 gtgggccagt acccgccccg gcgcttcagc gccaccgtga ccgatgggag ctccctcaga
     4501 gaggccctgg acacgatcgt cacgtcccgg acgaacgtgg ccgtggtcgt cacgaacggc
     4561 ggccgctacc gaggcgtcct gacgctggag aagctcacca gggagctgat cgcgtgacgg
     4621 gccgtccctt ctgggactgg gactgggtgg gccggaacgg tgacctcatc ctggaccagg
     4681 ccgtacagca cctctggctc acgttcctgg ccgtggtgat cgggctggcg atctcgttcc
     4741 cgctcgcggt gctggcctat cgtcgccgaa ggctctatcc ccccatcgcg tggctcaccg
     4801 ggacgctcta cacgatcccg agcctcgctc tcttcgcctt cctgactccc ttcaccggcc
     4861 tcagcacgct cacggcggag atcggcctcg tctcgtacac cctcctcatc ctgatccgga
     4921 acatcgtgac cggcctccgg gccgtgccgc ccgaggtccg ggaggcggcc gtcgggatgg
     4981 ggtacacgga ccgccagctc ctgtggagga tcgaactgcc gctcgcgctg cccgcggtgg
     5041 tggccggcct ccgcatcgcc acggtgacca cgatcgggct ggtcaccgtc acggcgctga
     5101 tcggccaggg cggtctcgga gcgctgatgc tgggcgggtt ccgacgcctc tacccggagc
     5161 tggccgtggt gctcggcgtg gggctgtcgg tcgcgctcgc cgtcgcgatc gacctgctgc
     5221 tgttcgccgg ccagcgatgg atcacgccct ggagccggcg ggccggggat cgagtggtcg
     5281 cgacgtgacc gagcccgggt tcttcagcca ggtggtggac tggttcgccg acgcggccaa
     5341 ctggagcggg gacttcggga tcccaagcag gctcctcgaa cacgtcgtca tgtcgggtgt
     5401 ctcggtggcg atcgcgacgg cgctcgccct gccgctggcg atgtacatcg gacatcggcg
     5461 ccggctggag cggctcgtcg tctcgatcac caacctcggg agggcgatcc cctccttcgg
     5521 gctgctgttc ctcttcgtgc tgtggctggg cgtcgggctc tcgagccctc ccgccctgcg
     5581 gcccgcgatc atcctggcgc tcgtcctgct ggcgatcccg ccgatcctca cgaacgccta
     5641 cgtcggcatc cagagcgtgg acgacgacac gctggaggcg gcccgcggca tgggcatgtc
     5701 cgagtggtcg atcctcttcc gggtcgaggt tcccctcggg atgcccctga tcgtggcggg
     5761 gcttcggacg gcggccgtcc aggtcgtggc caccgccacg ctgggcgccg tggtggccgg
     5821 cggagggctg ggccgctaca tcatcgacgg cttcgcggtc cgggacctcc ctcagatctt
     5881 cgcgggggcg atcctcgtcg cggcgctggc gctcgcggtg gacgtgctga tcggaggggt
     5941 ggggcggctc ctctcgccgc gtgtggccac gcgtcccggc gccgcgacgg agcgcggaat
     6001 cacggccgga gccgcgtagt tactaagatt gctgtcgccc atgttccagg ggggcctccg
     6061 gctccccgcg aagagagagg gggtacccat gcggaggacc ttccgcaccc ccgtccttgg
     6121 ggcggccctg gtcgcgctgg cgctgatcgc ggcggcgtgc gacgggtcgg gtgaggacgg
     6181 ggacggcgac ggggagtcgc ggggacagat caccgtgggc gccgtcgcct tcgccgagaa
     6241 ccagatcctc ggagagatgt acgcgcaggc gctggaggac gcgggctacg aggtcagccg
     6301 ccagctgagc ctcgactcgc gggagatcct ccagccggcg atcgag
//
LOCUS       DASUTW010000065         8319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1185656, whole genome shotgun sequence.
ACCESSION   DASUTW010000065 DASUTW010000000
VERSION     DASUTW010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8319
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185656"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1969
                     /locus_tag="VF058_02280"
     CDS             <1..1969
                     /locus_tag="VF058_02280"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX6207163.1"
                     /translation="PARSDPRSFPTGWGMEVRVDRAGDRPLGSLVWVSGRGEAARVAA
                     GDRVRGAGRFRRVEGEEGFERYLADRGVAALVSASELGREGPPRNPLLRVANGVRDAL
                     AAGARRALPPRRAGLLLGLTIGDTRGMHPETVEDFRATGLGHLVAVSGSNVVMVLAPV
                     VGLAALLGMGPRGRLAAGGLAVVLFALITRWEPSVLRAGVMAGLALLGVAAGRPRETA
                     TVLSWAVVILLLVDPRLSRSLAFQLSTAATVGLVALAPGIEARLRWLPRPVALAAAAT
                     AGAQAAVTPLLLGAFGIVPGSTLIANVLAFPAVAAAFLGGSTAAAVALVWPGLGSALG
                     TLASMPIAYLEAVADRTARLAVPALVGADWVPPVALAVVAHLVLRRVVAPRRAALLLG
                     APFLVWIVFVRGSPPPGLEVRFLDVGQGDAALVRTPRATILVDAGPDEQTVATRLAAL
                     GIGRLDLAVASHGHQDHVEGFPAVLARHPVGLLLDPGCPTGFPSHERMLDAAAYEDVP
                     IRHPRGGERLRVGDLLLEVLGPDECAPDSPNDDSIVLRLVHGSATVLLPGDAEVPAQL
                     DLLSDADPVRATVLKVPHHGSSTSDPSFFETVAASVAVVSVGEDNDFGHPVPDTLRAL
                     REAGGRVVRTDLAGEVTVTFEGDRPVVERSR"
     gene            2036..2440
                     /locus_tag="VF058_02285"
     CDS             2036..2440
                     /locus_tag="VF058_02285"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEX6207164.1"
                     /translation="MSDQKLSIVLFSGTDDKILGAIHLVAGATALGREVDVFLQFWAV
                     DAFRHDHINKDHGVTMDAGPEGPEMLERYGEIRWVETLHSVKEAGTLRITACSRCMEA
                     MGLAPEDMDELVDEVEGVTAFLSRATGQLIHM"
     gene            2488..3435
                     /gene="holA"
                     /locus_tag="VF058_02290"
     CDS             2488..3435
                     /gene="holA"
                     /locus_tag="VF058_02290"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX6207165.1"
                     /translation="MPEPVVLVWGESDFLLREAATRLFEGARPTEVEGAAWRPGLTAD
                     LATPSLLGEERALLVTGLQAAPSDALDEIARYALEPNPEARLVLACVVSPRAKGPPAK
                     VMKLLQGRARIERIAIDRKDLARWVADRARKAGLKVTPAGAAALVETLGEDPSVLAQS
                     VSQIADAFPEEGLTPSTVAAQFRGFGERRIFELCDAAFGGNARDALRYLAAMLGAREQ
                     PLAILGGMAARVRDLLRVRSLPPSMPLQDVAREAGLRFDWQARRYREQAGRYDPEDLA
                     DIHRRVVEADAQLKQGGGGDVVLTMLVTRIANGRARVSR"
     gene            complement(3438..3707)
                     /gene="rpsT"
                     /locus_tag="VF058_02295"
     CDS             complement(3438..3707)
                     /gene="rpsT"
                     /locus_tag="VF058_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010468502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX6207166.1"
                     /translation="MANIQSQIKRNRQNERRRMRNKAVRTRLKTEAKRTLEVAEQGDL
                     EAARSAYRETARELDRAASKGVLHPRTAARRKSRLAKRLNAQQGE"
     gene            3766..5577
                     /gene="lepA"
                     /locus_tag="VF058_02300"
     CDS             3766..5577
                     /gene="lepA"
                     /locus_tag="VF058_02300"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003985019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX6207167.1"
                     /translation="MTHPIRNFAIISHIDHGKSTLADRFLELTHAVAERQMREQFLDR
                     MDLERERGITIKAQAVRLAYGLDGEPYVLNLIDTPGHVDFTYEVSRALAAAEGAILLV
                     DAAQGIEAQTLANYHLAEQAGLTILPAVNKIDLPQADVEGAAREIAELTGVPQDEVLH
                     ISGKTGEGVGELLRAVVERIPPPRGDPDAPTRALIFDSTYDQYRGVVAYVRVVDGTLP
                     SNARVRMVATGHETDAEEVGVFAPDPVAATALTAGEVGYLVTGVKDVRQAKVGDTITL
                     ARTEVEPLPGYREPKPMVWSGLFPEDGDEYPALRDALDRLQLNDAALVFEPETSRALG
                     FGFRTGFLGLLHMEIVRERLEREFGLQLIATAPNVELHVAREGGETLEVHNPSEMLVP
                     GTYERVEEPYVLATILTPSAYLGPVMELAQDRRGSMEGMRYLSPERVEIRYLLPLAEI
                     IFDFFDQLKSGTRGYASLDYEPFGYQEGDLVRVDLLLHGEPVDAFSAVVHRDKAQAYG
                     RRMVERLKELIPRQLFDVAVQAAIGGRIVARETVRAKRKDVTAKCYGGDITRKRKLLE
                     RQREGKRRLRRVGKVDVPHEAFIAALRVDTEAGGKGR"
     gene            5574..6695
                     /gene="hemW"
                     /locus_tag="VF058_02305"
     CDS             5574..6695
                     /gene="hemW"
                     /locus_tag="VF058_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX6207168.1"
                     /translation="MKRPEARAGDAGIYVHVPFCLTRCGYCDFNAHAGLDHLKSPFVE
                     ALLTEVELAAPSWEGVRVVSIFLGGGTPTTLSPEALGSIVDRLRERFDLAPDAEVTCE
                     ANPDTVDAPSLRRLRERGVTRLSMGAQSFDPAVLSALQRIHSVSSVRRAVDDARAAGF
                     DDVSLDLIYGARGETLASWEGTLDEALALGPDHLSCYALTIERGTPLGREVALGLAAP
                     PDPDLQADMYEAACTRLGGAGYEHYELSNWARPGAASLHNLGYWTGRPYLGLGPGAHS
                     FREGVRRWNLRSPERYVEELRGGRLPTGGEERPSEDERRMERLLLGLRLAEGVPASWL
                     PIDVAEDLFARGLARRDDGRVALTDPGMLLANEVVLSSS"
     gene            6732..7760
                     /gene="hrcA"
                     /locus_tag="VF058_02310"
     CDS             6732..7760
                     /gene="hrcA"
                     /locus_tag="VF058_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX6207169.1"
                     /translation="MSIEERKAAVLRAVVRDHIRTGEPVGSKTIVSRYGLGVSAATIR
                     NELAALEELGYLAQPHTSAGRIPTDLGYRFYVDSLPETVALPRTLQEQIETFFGPPAA
                     DVDEVLHGTASLLSRLTGYAAVAVTPRATTSRVLRTELVPVGSSVMLLIVSDTGRVDK
                     RMLEVGHEPDERIVERASRMLSERLAGLSYDEAARRVAALIREMADEERRLFRDVATA
                     IRHMGDASGEIYLGGVANIADEGAFESPDTLREMVGALEEGSAVLEILRAASGAIDDV
                     TVRIGRENTLRAMAEASVVMAWYGPRSRPVGAVAVVGPTRMEYPRAISSTRVVAGHLS
                     DLLRGLAG"
     gene            7764..>8319
                     /locus_tag="VF058_02315"
     CDS             7764..>8319
                     /locus_tag="VF058_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX6207170.1"
                     /translation="MAQTRDLYEVLGVSRGATQDEIRKAYRRLVREYHPDVSDDPAAE
                     HRIKEINLAYQTLSDPARRRQYDMYGGEGFTPDMFDFMGDVSDIFEMFFGSPWRGRRS
                     RRPERGEDARLAIALDFEEAAFGVEKEIEVETLAPCEVCDGSGREPGTEAVRCAHCGG
                     NGEVSDVRRSVFGTVMTSRICSACG"
BASE COUNT         1058 a         2908 c         3159 g         1194 t
ORIGIN      
        1 accggcgcgc tccgacccca ggagctttcc taccggctgg gggatggagg tccgcgtcga
       61 tcgggcaggg gatcgacccc tggggagcct agtgtgggtc tcgggtcgcg gggaggccgc
      121 ccgggtggcg gcgggggacc gggtgcgggg cgcgggccgc ttccgccgcg tcgaggggga
      181 ggaggggttc gagcgatacc tggccgatcg gggggtggcc gcgctcgtgt cggcgtcgga
      241 gctggggcgg gagggcccgc cgcgcaatcc gctcctccgc gtcgcgaacg gggtccgcga
      301 cgcgcttgcc gccggggcgc ggcgggccct cccgccccgc agggcggggc tcctgctcgg
      361 cctgacgatc ggggacacgc gcgggatgca ccccgagacc gtggaggact tccgggccac
      421 ggggctgggg cacctcgtcg ctgtctcggg atcgaacgtc gtgatggtcc tggcgccggt
      481 ggtgggcctg gccgcgctcc tggggatggg cccccggggg aggctcgcgg cggggggcct
      541 tgcggtcgtc ctgttcgccc tcatcacgag gtgggagccc tcggtcctgc gggccggcgt
      601 gatggcgggc ctggccctgc tcggggtggc ggcggggcgg ccccgggaga cggccaccgt
      661 cctgtcctgg gcggtggtga tcctgctgct ggtggacccg aggctctcgc gatcgctcgc
      721 gttccagctc tcgaccgccg cgacggtggg gctcgtggcg ctcgcgccgg ggatcgaggc
      781 gcggctgcga tggctccccc gcccggtggc cctcgcggcc gccgccacgg cgggggcgca
      841 ggcggccgtg acccccctcc tgctgggggc gttcgggatc gtgccgggct cgaccctgat
      901 cgccaacgtg ctggccttcc ccgccgtggc ggcggccttc ctggggggct cgactgccgc
      961 ggcggtcgcc ctcgtctggc ccggtctcgg ctccgccctc ggcacgctcg cctcgatgcc
     1021 gatcgcctac ctggaggcgg tcgcggaccg cacggcgcgg ctcgccgtcc ccgcgctggt
     1081 gggcgccgac tgggtgcccc ccgtggccct ggcagtcgtc gcgcacctcg tcctgcggcg
     1141 cgtggtggct ccgcgccgcg ccgccctgct cctgggagcg cccttcctcg tctggatcgt
     1201 gttcgtacgc ggctccccgc cgcccggcct cgaggtgcgg ttcctcgacg tggggcaggg
     1261 cgacgcggcg ctcgttcgga cgccccgggc cacgatcctg gtggacgccg ggcccgacga
     1321 gcagaccgtg gccacccgcc tggccgctct cgggatcgga cgcctcgacc tcgccgtggc
     1381 ctcgcacggc catcaggacc acgtcgaggg gttccctgcg gtgttggccc gtcatcccgt
     1441 cgggctcctc ctcgacccgg ggtgccccac gggattcccc tcccacgagc ggatgttgga
     1501 tgccgccgcg tacgaggacg ttcccatccg gcacccccgc ggcggggagc gcctgcgggt
     1561 gggggacctc ctgctcgagg tcctgggtcc cgacgagtgt gcgcccgact cgccgaacga
     1621 tgactcgatc gtcctgcgtc tcgtccacgg gtcggccacg gtcctcctgc cgggcgacgc
     1681 ggaggtcccg gcccagctcg acctgctctc cgacgccgat cccgtgcggg ccacggtcct
     1741 caaggtccct caccacggct cctccacctc ggatccatcg ttcttcgaga cggtcgcggc
     1801 ctcggtcgcc gtcgtcagcg tgggcgagga caacgacttc ggccaccccg tgccggacac
     1861 gctccgggcc ctgcgggagg cgggcggaag ggtcgtgcgg accgacctgg ccggcgaggt
     1921 gaccgtgacc ttcgagggcg accggccggt ggtcgagagg tcccggtgaa cgcgggcctc
     1981 acgacccggc ccggagaccc cgccgcctgc caggctgaac gctggaggtg gtggcatgtc
     2041 ggaccagaag ctctcgatcg tgctgttctc gggcaccgac gacaagatcc tcggggccat
     2101 acacctcgtg gcgggggcca cggccctcgg ccgggaggtc gacgtcttcc tccagttctg
     2161 ggccgtggac gcgttccgtc acgaccacat caacaaggac cacggcgtga cgatggacgc
     2221 cggacccgag ggacccgaga tgctcgagcg gtacggggag atccgctggg tggagaccct
     2281 ccacagcgtc aaggaggccg gcacgctgcg catcacggcg tgctcgcgct gcatggaggc
     2341 catggggctc gccccggagg acatggacga gctcgtggac gaggtcgagg gcgtgaccgc
     2401 gttcctctcc cgcgccacgg gccagctgat ccacatgtga cgcggccggg gccctccggg
     2461 cgaggtagca tccgcccgtg atgccgagtg cctgagcccg tggtcctcgt ctggggcgag
     2521 agcgacttcc tgctgcgcga ggccgcgacg aggctgttcg agggagcccg ccccaccgag
     2581 gtggagggcg ctgcctggag gcccggcctc acggccgacc tggccacgcc ctcgctcctc
     2641 ggggaggagc gggccctgct ggtgacgggc ctccaggcgg cgccctccga cgccctcgac
     2701 gagatcgcgc gctacgcgct ggagccgaac cccgaggcga ggctcgtcct ggcctgcgtc
     2761 gtctccccgc gggccaaggg gcccccggcc aaggtcatga agctcctcca gggccgcgct
     2821 cgcatcgagc gcatcgcgat cgaccgcaag gaccttgctc gctgggtcgc ggaccgggcc
     2881 aggaaggccg gcctcaaggt cacgccggcc ggggccgccg cgctcgtgga gaccctgggc
     2941 gaggacccct cggtgctcgc gcagtccgtg tcccagatcg ccgacgcctt ccccgaggag
     3001 ggtctcacgc cgtccaccgt cgccgcgcag ttccgcgggt tcggcgagcg acggatcttc
     3061 gagctgtgcg acgcggcgtt cgggggcaac gctcgcgacg ccctccgcta cctcgccgcg
     3121 atgctggggg cccgggagca gccgctggcg atcctggggg gtatggccgc ccgcgtccgg
     3181 gacctgctgc gggtgcggag cctccccccg agcatgccgc tgcaggacgt ggctcgcgag
     3241 gccggcctgc ggttcgattg gcaggcccgc cgctaccggg agcaggcggg gaggtacgac
     3301 cccgaagatc tcgcggacat ccatcggaga gtggtcgagg ccgacgctca gctgaagcag
     3361 ggaggcggcg gcgacgtggt cctgacgatg ctggtgacgc ggatcgcgaa cgggagggcg
     3421 cgggtctcgc gctgaggcta ctcgccctgc tgggcgttga gccgcttggc caggcgcgac
     3481 ttgcggcggg ccgccgttcg cgggtgaagg acgcccttcg aggcggcccg gtccagctcc
     3541 cgggcggtct cgcggtaggc ggagcgagcg gcctcgaggt cgccctgctc ggcaacctcc
     3601 agcgtccgct tggcctcggt cttcaggcgc gtgcgaacgg ccttgttgcg catgcgacgg
     3661 cgctcgttct ggcggttccg cttgatctgg gactggatgt tggccatcgg cgggcgattg
     3721 tagcaggtcg tcggggtcga gccccgggcg gctaccatgg cgagcgtgac ccacccgatc
     3781 cggaacttcg ccatcatcag ccacatcgac cacgggaagt ccacgctggc cgaccgcttc
     3841 ctggagctga cccacgccgt ggccgagcgc cagatgcgcg agcagttcct ggaccggatg
     3901 gacctcgagc gcgagcgggg gatcacgatc aaggctcagg cggtgcggct ggcctacggg
     3961 ctcgacgggg agccctacgt gctcaacctg atcgacacgc ccggccacgt ggactttacc
     4021 tacgaggtct cgcgggcgct ggcggcggcc gagggcgcca tcctgctcgt ggacgcggcg
     4081 caggggatcg aggcccagac cctcgccaac taccatctcg cggagcaggc cgggctcacg
     4141 atcctgccgg cggtgaacaa gatcgacctc ccgcaggcag atgtggaggg cgccgctcgg
     4201 gagatcgcgg agctcaccgg cgtcccccag gacgaggtgc ttcacatctc ggggaagacg
     4261 ggggaggggg tcggggagct gctgcgggcg gtggtcgagc ggatcccccc gcctcgcggg
     4321 gaccctgacg cccccacccg ggccctgatc ttcgactcca cctacgacca gtaccgaggc
     4381 gtggtggcct acgtacgcgt ggtggacggg acgctcccgt ccaacgcccg ggtccggatg
     4441 gtggccacgg gccacgagac cgacgccgag gaggtgggcg tcttcgcccc cgaccccgtg
     4501 gccgccacgg cgctgaccgc cggggaggtg ggctacctcg tgaccggggt gaaggatgtc
     4561 cgccaggcga aggtcggcga cacgatcacg ctcgcccgga ccgaggtgga gccgctccca
     4621 gggtaccggg agccgaagcc gatggtctgg tcggggctgt ttcccgaaga cggcgacgag
     4681 tacccggccc tgcgcgacgc gctcgaccgt ctccagctga acgacgcggc gctcgtgttc
     4741 gagccggaga cttcgcgggc gctcgggttc gggttccgta ccggcttcct cggactgctc
     4801 cacatggaga tcgtccggga gcgcctggag cgggagttcg ggctccagct gatcgccacg
     4861 gccccgaacg tggagctgca cgtggcccgc gagggcgggg agacgctgga ggtccacaac
     4921 ccctcggaga tgctcgtccc cggcacctac gagcgcgtcg aggagcccta cgtgctcgcc
     4981 acgatcctca cgccgagcgc ctacctgggc cccgtcatgg agctggccca ggaccggcgg
     5041 gggtcgatgg aggggatgcg ctacctctcg cccgagcggg tcgagatccg ctacctgctc
     5101 ccgctggccg agatcatctt cgacttcttc gaccagctga agtcggggac gcgcggctac
     5161 gcctcgctcg actacgagcc cttcggctac caggagggag acctcgtgcg ggtggacctc
     5221 ctcctccacg gggagccggt ggacgcgttc tcggcggtcg tgcatcggga caaggcgcaa
     5281 gcctacggcc gccggatggt cgagcggctg aaggagctga tcccgaggca gctcttcgac
     5341 gtcgcggtgc aggcggcgat cggaggtcgc atcgtggcgc gggagaccgt ccgcgccaag
     5401 cgcaaggacg tgacggcgaa gtgctacggg ggggacatca cccggaagcg caagctgctg
     5461 gagcgccaga gggaggggaa gcggcggctg cggcgcgtgg ggaaggtgga cgtgccgcac
     5521 gaggccttca tcgccgcgct ccgtgtggac accgaggcgg gggggaaggg tcgatgaagc
     5581 ggccggaggc gcgggccggg gacgccggga tctacgtcca cgtccccttc tgccttaccc
     5641 gctgcgggta ctgcgacttc aatgcccacg cggggctgga tcacctgaag tccccgttcg
     5701 tggaggccct gctgacggag gtcgagctgg cggcgccctc ctgggagggg gtccgcgtgg
     5761 tctcgatctt cctgggcggg gggacgccga ccaccctgtc tcccgaggcg ctgggatcga
     5821 tcgtggatcg gctccgcgag cgtttcgacc tggcgcccga cgccgaggtg acctgcgagg
     5881 cgaaccccga cacggtggac gctccgtcgc tgcgccggct ccgggagcgc ggcgtcaccc
     5941 gcctctcgat gggcgcgcag tccttcgacc ccgccgtgct gtcggcgctc cagcggatcc
     6001 actcggtctc gtcggtgcgc cgggcggtcg acgacgcacg ggccgccggg ttcgacgacg
     6061 tcagcctcga cctgatctac ggcgctcggg gggagacgct cgcgtcgtgg gagggcacgc
     6121 tggacgaggc gctggcgctg gggcccgacc acctgtcgtg ctacgcgctc acgatcgagc
     6181 gggggacccc gctgggacgc gaggtcgccc tggggctcgc cgcgccgccc gaccccgatc
     6241 tccaggctga catgtacgag gcggcctgca ccaggctcgg gggcgccggc tacgagcact
     6301 acgagctctc gaactgggcg cggcccggcg cggccagcct gcacaacctc ggctactgga
     6361 ccggtcgtcc gtacctgggg ctcgggccgg gagcccactc gttccgcgag ggcgtccgcc
     6421 ggtggaacct ccgctcgccc gagcggtacg tggaggagct ccgcggcggc aggctcccca
     6481 cggggggtga ggagcggccg agcgaggacg agcggcggat ggagcgtctc ctgctgggcc
     6541 tccgcctcgc ggagggagtt ccggcgtcgt ggctcccgat cgacgtggcc gaggatcttt
     6601 tcgctcgcgg cctggcgcgg cgcgacgacg gccgcgtggc cctgacggat cccgggatgc
     6661 tgctcgccaa cgaggtcgtc ctgtcgtcgt cctagcactc gggccgggtg agtgctaaaa
     6721 ctgtaggcag catgtccatc gaggagcgca aggcggcggt cctgcgagcc gtggtccgcg
     6781 accacatccg gacgggcgag cccgtgggga gcaagacgat cgtgagccgg tacgggctcg
     6841 gggtcagcgc cgccacgatc cgcaacgagc tggccgcact cgaggagctc ggctacctgg
     6901 cgcagcccca cacctcggcg gggcgcatcc ccacggacct cggctaccgg ttctacgtcg
     6961 actccctccc cgagacggtc gccctccctc gaacgctcca ggagcagatc gagaccttct
     7021 tcggcccgcc ggccgccgac gtggacgagg tcctgcacgg caccgcgtcc ctcctctcga
     7081 ggctcaccgg atacgccgcg gtggccgtca cgccccgggc gacgaccagc agggtcctgc
     7141 gcaccgagct cgtgccggtg ggctccagcg tgatgctgct gatcgtcagc gacacgggca
     7201 gggtcgacaa gcggatgctc gaggtgggcc acgagcccga cgagcggatc gtggagcgcg
     7261 cctcgcggat gctctccgag cggctggccg ggctctccta cgacgaggcg gcgcgacgcg
     7321 tggcggctct gatacgggag atggccgacg aggagcggcg gctcttccgc gacgtggcga
     7381 cggcgatccg gcacatgggt gatgcttccg gcgagatcta tctggggggc gtggccaaca
     7441 tcgccgatga gggggcgttc gagtcgcccg acaccctccg ggagatggtg ggcgcgctgg
     7501 aggagggctc ggcggtcctg gagatcctcc gggcggcctc aggcgcgatc gacgacgtca
     7561 cggtccggat cgggcgggag aacacgctcc gggcgatggc cgaggccagc gttgtgatgg
     7621 cctggtacgg gccccgatcc cgcccggtcg gcgcggtcgc ggtggtgggc cccacccgca
     7681 tggagtaccc gagggcgatc tcgtcgaccc gcgtggtggc cgggcacctc tcggacctgc
     7741 tccgcgggct cgccggatag gccatggccc agacgcgcga cctctacgag gtcctgggcg
     7801 tgtctcgggg cgccacccag gacgagatcc ggaaggccta ccggcggctc gtgcgcgagt
     7861 accaccccga cgtcagcgac gaccccgccg ccgagcaccg catcaaggag atcaacctcg
     7921 cctatcagac cctgtcggac ccggcgcgtc gacgtcagta cgacatgtac gggggcgagg
     7981 gcttcacgcc cgacatgttc gacttcatgg gcgacgtctc ggacatcttc gagatgttct
     8041 tcgggagccc gtggcgaggc cgtcggtccc ggcgcccgga gcgcggcgag gacgcccgcc
     8101 tggcgatcgc gctcgacttc gaggaggccg ccttcggcgt ggagaaggag atcgaggtcg
     8161 agacgctcgc cccgtgcgag gtgtgcgacg gctcgggtcg ggagccgggt accgaggcgg
     8221 tccgctgcgc ccactgcggg gggaacgggg aggtctccga cgtgcgtcgc agcgtcttcg
     8281 ggacggtgat gacgtcgagg atctgctcgg cgtgcgggg
//
LOCUS       DASUTW010000066         3627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_796405, whole genome shotgun sequence.
ACCESSION   DASUTW010000066 DASUTW010000000
VERSION     DASUTW010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796405"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1011)
                     /locus_tag="VF058_02320"
     CDS             complement(49..1011)
                     /locus_tag="VF058_02320"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX6207171.1"
                     /translation="MEITVGTFNLNNLFSRFNFRARVDAIPEEERDVTVSFEFTERGE
                     YWFRTFRGRLIDPMPEEQRRRLAARIKTMDLDVLAVQEVEDVEALRDFNRFHLEGMYP
                     HAVLVEGNDARFIDVAVLSKLPLGGATSWQHEVHPEDPSRPVFARDLLQVEVLNGPRT
                     RRLLTMFINHLTSKFISFGENPVAAAAAKTARRRRQAETVERIVERQLRPDSPFVILG
                     DMNDTPDSEALTPLAQGSLGLVDGLADPEETRASKPDDPPPATKAWTHRFKEGGQPAR
                     YELLDQIWLSPSLAPKQTGAFIERRTLHGGDGSDHDPAWVVLSI"
     gene            1128..1469
                     /locus_tag="VF058_02325"
     CDS             1128..1469
                     /locus_tag="VF058_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207172.1"
                     /translation="MGVREPLEPGMIPGLCERLDAILDERNVGLVLCAVVGPRKATAV
                     TVEALARLRIVARRRRRRLRVVHPWPELRGLVALMGLEELLLLQPEGEPEEREQPRGV
                     EEEGEAADPPS"
     gene            complement(1380..2375)
                     /locus_tag="VF058_02330"
     CDS             complement(1380..2375)
                     /locus_tag="VF058_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014057013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6207173.1"
                     /translation="MSDLASPRQKAPEDLETILEQHRRELIAHCYRMLGSPFEAEDAV
                     QETMLRAWRAFDRFEGRSSVRSWLYRIATNVCLDILEGRGRRALPMDLGPAGSARMPL
                     PEPLPEVTWVQPIPDGLAVASEGDPAELAEQRDSIRLALVAALQHLPPRQRAVLILRE
                     VLRWKAAEVAELLETSVASVNSALQRARATLASAGEDTARSVESMDEERRELLSRYVD
                     AFERYDMDALTSLLHDDAKWSMPPYELWLQTHEDVVAWCLGPGVGCRGSRLVPVEANG
                     VPAFGQYKPSESGEGYEPWSLQVPEISGGRISGLTFFLDTARLFPLFGLPLRLEQ"
     gene            2646..>3627
                     /locus_tag="VF058_02335"
     CDS             2646..>3627
                     /locus_tag="VF058_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6207174.1"
                     /translation="MTLKQAIRDLRDRFTGHVMGPEDEGYEQARTVVAGDVDKRPAVI
                     VRPTDAEDVTRVIALARETGLELAVRSGGHSGAGHSTTEGGIVLDVRDMKGLEIDARG
                     RTAWAEAGLTAGEYTTAAGAHGLATPFGDTGTVGIGGITLAGGIGLLVRRYGMTIDSL
                     LAAEIVTADGEVLRVDEEQHPDLFWAIRGGGGNFGVVTRFLLRLHPVDTVVGGMLLLP
                     ATPEVVAGFIEEAERAPEELSTIANVMPAPPMPFVPEDRHGELVVLALMVHAGDVGDG
                     ERAVAPFRSLAEPVADLLRPMPYSEMFPPEEEEYRPTAVAQTMLIQEIDAG"
BASE COUNT          529 a         1259 c         1318 g          521 t
ORIGIN      
        1 ggaccccgac ggcaacctct ggctcctcca ggaggtcccc agccgcgact agatcgagag
       61 cacgacccac gccgggtcgt ggtcgctccc gtcgccgccg tgcagcgtgc ggcgctcgat
      121 gaacgcgccg gtctgcttcg gggcgaggct ggggctcagc cagatctggt cgaggagctc
      181 gtagcgggcc ggctgcccgc cctccttgaa gcgatgggtc cacgccttcg tggccggcgg
      241 cgggtcgtcg ggctttgacg cacgggtctc ctccggatcg gccaggccgt ccacgagccc
      301 cagcgacccc tgcgccaggg gggtcagcgc ctcggagtcg ggtgtgtcgt tcatgtcgcc
      361 caggatcacg aacggggagt cggggcggag ctgcctctcg acgatgcgct ccaccgtctc
      421 ggcttgccgc cgccgccgcg cggtcttcgc ggcggcggcg gcgaccgggt tctcaccgaa
      481 cgagatgaac ttgctcgtga ggtggttgat gaacatcgtc agcagccgcc tggtgcgcgg
      541 cccgttcagg acctcgacct gcaggaggtc gcgggcgaag acggggcgcg agggatcctc
      601 cggatgcacc tcgtgctgcc acgacgtggc cccgcccagc ggcagcttcg agaggaccgc
      661 cacgtcgatg aagcgggcgt cgttcccctc gaccagcacg gcgtgcgggt acatgccctc
      721 gaggtggaac cggttgaagt cccggagggc ctccacgtcc tcgacctcct ggacggcgag
      781 cacgtcgagg tccatcgtct tgatgcgcgc ggcgaggcgc cggcgctgct cctccggcat
      841 cgggtcgatc agccgccccc ggaaggttcg gaaccagtac tccccccgct ccgtgaactc
      901 gaagctgacc gtgacgtcgc gctcctcctc cgggatcgcg tccacccgcg cccggaagtt
      961 gaagcgcgag aacaggttgt tgaggttgaa cgtcccgacc gtgatctcca tgattcctcc
     1021 tcacgggctc cacgagcgac ggtaggtcat cttccgcgat gagttccgcg cccggccccc
     1081 gtcatttgag aggaggaagg aggtcggcgc tgacccacgc gctggtcgtg ggggtccggg
     1141 agccgctcga gccggggatg atcccggggc tctgcgagcg cctcgacgcg atcctcgatg
     1201 agcgaaacgt cggcctcgtc ctgtgcgcgg tcgtgggccc gcggaaggcg accgccgtca
     1261 ccgtcgaggc cctggcgcgg ctccggatcg tggcccgcag acggaggcgc cgcctccgcg
     1321 tcgtgcaccc gtggcccgag ctccgcggcc tcgtcgctct catgggcctg gaggagctgc
     1381 tactgctcca gccggagggg gagcccgaag agcgggaaca gcctcgcggt gtcgaggaag
     1441 aaggtgaggc cgctgatcct ccctcctgag atctcgggca cctggagcga ccacggctcg
     1501 tagccctcgc cggactccga cggcttgtac tgcccgaacg ccgggacgcc gttggcctcg
     1561 accggtacga ggcgggagcc ccggcacccg acccctgggc cgaggcacca ggccacgacg
     1621 tcctcgtggg tctggagcca gagctcgtac gggggcatcg accacttcgc gtcgtcatgc
     1681 aggagcgagg tgagcgcgtc catgtcgtac cgctcgaaag cgtcgacgta gcgggagagc
     1741 agctcgcggc gctcctcgtc catcgactcc acggacctcg cggtgtcctc ccccgccgag
     1801 gcgagcgtcg cccgggcccg ctgcagggcg ctgttcaccg aggccaccga ggtctccagc
     1861 agctcggcca cctcggccgc cttccaccga aggacctcgc gcaggatcag gaccgcacgc
     1921 tggcggggcg ggaggtgttg gagggcggcc acgagcgcca ggcggatcga gtcccgctgc
     1981 tcggcgagct cggcggggtc gccctcggat gcgacggcga gcccgtcggg gatgggctgg
     2041 acccacgtca cctcggggag cggctcgggg agcggcatcc gggccgaccc cgctggcccc
     2101 aggtccatcg ggagggccct tcgtcccctc ccctccagta tgtccaggca cacgttggtg
     2161 gcgatgcggt acagccatga gcgcaccgag gagcggccct cgaaacggtc gaacgccctc
     2221 cacgccctga gcatggtctc ctgcactgcg tcctcggcct cgaacgggga gccgagcatc
     2281 cggtagcagt gcgcgatcag ctcgcggcgg tgctgctcca ggatcgtctc gaggtcctcg
     2341 ggggccttct gtcggggtga agccagatcg ctcatggggt tcgctcctcg tgggcgggtc
     2401 cccacatcat atcgacgacg acgcggggat cggggacctg ccgggggacg acgacgttct
     2461 tcatcaggga agaggaggga tcggcgctgg ggttcctgat caaccgatga gcttcgacgc
     2521 caccaaccgt cgtatccggg acgcgacgac gagcggtgaa agctcttcga agcctccgcg
     2581 gtcggctccg atgcgttccc gggcccgcga ccgtcataag agagagcacg agagaaggga
     2641 gcacgatgac gctgaagcag gcgatccgcg acctccgaga ccgcttcacc ggacacgtga
     2701 tggggcccga ggacgagggc tacgagcagg cccgcacggt cgtcgcgggc gacgtggaca
     2761 aacggcccgc cgtgatcgtc cggcccacgg acgccgagga cgtgacgcgc gtgatcgccc
     2821 tggcccggga gaccgggctg gagctggccg tgaggagcgg ggggcacagc ggcgcagggc
     2881 acagcacgac ggagggcggg atcgtcctcg acgtccgcga catgaaggga ctcgagatcg
     2941 acgcccgggg acgcaccgcc tgggccgagg cggggctgac cgccggcgag tacacgacgg
     3001 ccgcgggggc ccacggcctg gccacgccct tcggcgacac ggggacggtg ggcatcggcg
     3061 gcatcacgct ggcggggggc atcggcctcc tcgtccggcg gtacggcatg acgatcgaca
     3121 gcctcctcgc cgccgagatc gtcacggccg acggcgaggt gctccgcgtg gacgaggagc
     3181 agcatccgga tctcttctgg gcgatccggg gcgggggcgg caacttcggc gtcgtcacca
     3241 ggttcctgct ccggctgcac cccgtcgaca ccgtcgtggg cgggatgctg ttgctcccgg
     3301 ccaccccgga ggtcgtggcc gggttcatcg aggaggccga gcgcgccccc gaggagctgt
     3361 ccacgatcgc gaacgtgatg ccggctcccc cgatgccctt cgtccccgag gaccggcacg
     3421 gcgagctcgt ggtgctcgcc ctcatggtcc acgccgggga cgtgggagac ggcgagcggg
     3481 ccgtcgcccc gttccggtcg ctcgccgagc ccgtcgcgga cctgctgagg cccatgccgt
     3541 actcggagat gttccctccc gaggaggagg agtaccgccc cacggcggtc gctcagacga
     3601 tgctgatcca ggagatcgac gcaggcg
//
LOCUS       DASUTW010000067         4190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_408299, whole genome shotgun sequence.
ACCESSION   DASUTW010000067 DASUTW010000000
VERSION     DASUTW010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408299"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VF058_02340"
     CDS             <1..603
                     /locus_tag="VF058_02340"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823589.1"
                     /note="catalyzes the NADP-dependent rearrangement and
                     reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to
                     2-C-methyl-D-erythritol 4-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX6207175.1"
                     /translation="DELEKASVKEALAHPTWKMGPKITIDSATLMNKGLEIIEAHYLF
                     ALEYSRIDVVVHPESIVHSLAEFGDGSTMAQLSNPDMRLPIQLALGFPDRMPTGVEPL
                     DLPSLGRLTFEELDREAFPAVDLCYQAGRAGLTYPAVLNAANEVAVMAFLEGKIQLTG
                     ITDVVSQVIDEHEPAGVVSVVYLDRADTWARQRAAELIEQ"
     gene            604..1803
                     /locus_tag="VF058_02345"
     CDS             604..1803
                     /locus_tag="VF058_02345"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX6207176.1"
                     /translation="MRLLRGFLGFLVGLLALNLLLFLVVPEGWRGVVLFIEALLLMIL
                     IHEAGHFFTAKWFGIKVEEFFVGFGPRLWSTRRGETEYGIKAIPAGGYVRIAGMNPFQ
                     EPAPEDRDRIFGAKPAWQRAIVLAAGSFTHFVLAILLLASIVAFLGVPVEGPPLVGTV
                     EARLAGDVSPAASSGMEEGDQIVEVNGEPIDSNEELVEITSGSEGEEMAIVVLRDGER
                     VTLRATPVLDEIQGEQVARLGIRIEPPRETEPVHLALWRGVEGTGEIAVESFKALGRV
                     FRPSGLARIGELVTGQAERSVEDPLSIVGAGRLAGQAASTDNYDILLAFLAALNVFVG
                     IVNILPLPPLDGGHLAVLAWEKVTGRKVDMARLIPLTAAVAGLLILLTLALAYLDVAN
                     PVPDPFR"
     gene            1892..3163
                     /locus_tag="VF058_02350"
     CDS             1892..3163
                     /locus_tag="VF058_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207177.1"
                     /translation="MEVGIMRLIGSERRLLGPALVVAWTMALGPQASGAVPGDLDPTF
                     DGDGMRVVDLGGSEESYAVATRRNGKTLIAGTTNEGGDNDVVVIQLTSGGVPDSSFGG
                     GDGVVTTDFFGGYDSAYAVELLPKGKFLVAGQALDGQGKSRFAVARFRRSGTLDTSYG
                     SKGKRTVAFPGNAVALDMVRLPGGSVVLVGASYPNENFVLARLTKGGRVMKSFGKKGR
                     VITNLGGDDGAHAGALQDDRIVVAGGSGTSFATQNVALARYRLGGGLDRTFAGNGKKV
                     VSMVAARDWANDLVVLPNGRIVVVAYASGDIGLARFKPRGGLDRSFGGGDGKVLADFG
                     DDATGQALGRSGKKLVVAGSIGFPPDPAVFRFGPGGGIDGTFGSGGMTMADFGPDIAA
                     AWDVVVRGTKILIAGYTETPGGDIVAARFLG"
     gene            3213..>4190
                     /gene="ispG"
                     /locus_tag="VF058_02355"
                     /gene_synonym="gcpE"
     CDS             3213..>4190
                     /gene="ispG"
                     /locus_tag="VF058_02355"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017608683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX6207178.1"
                     /translation="MQTERRGSRKVKVGWVEVGGDAPVRVQSMTTTKTADVNATLQQM
                     ASLVAAGCEIVRVAVPHREDAEALKQIAEHATIPVIADIHFQWRYAMMALEAGIHGLR
                     INPGNIKYQDKVRLIAREAQARGVPIRIGVNAGSLEKDLLAKHGRPTPQALVESAMNE
                     VRILEDEGFADIKISVKHSDPREMIEAYRLLAERCDYPLHLGVTEAGPMPAGGVKSAV
                     GIGTLLAEGIGDTIRVSLTDDPVEEVKVGNWILRSLGLKELGLDLVACPSCGRAEVDV
                     LGLTKQVNEALEREGITEPMRVAVMGCVVNGPGEAREADLGIAAGKGYGQ"
BASE COUNT          638 a         1382 c         1557 g          613 t
ORIGIN      
        1 gacgagctgg agaaggcctc ggtgaaggag gccctggccc acccgacctg gaagatgggc
       61 cccaagatca cgatcgactc ggccacgctg atgaacaagg gcctcgagat catcgaggct
      121 cactacctct tcgccctgga gtactcgagg atcgacgtcg tcgtgcaccc cgagagcatc
      181 gtccacagcc tcgccgagtt cggcgacggc agcaccatgg cccagctctc gaaccccgac
      241 atgcgcctgc cgatccagct cgcgctggga ttcccggacc gcatgccgac cggcgtcgag
      301 ccgctggacc tcccctcgct ggggcgcctc accttcgagg agctcgatcg cgaggcgttc
      361 cccgccgtag acctctgcta ccaggcgggg cgggcgggcc tgacgtatcc ggcggtgctc
      421 aacgccgcca acgaggtcgc ggtcatggcc ttcctcgagg ggaagatcca gctgaccggc
      481 atcaccgacg tcgtgtcgca ggtcatcgac gagcacgagc ccgcgggggt cgtgtcggtc
      541 gtgtacctcg accgcgcgga cacctgggcg cgccagcgcg cggccgagct catcgagcag
      601 tagatgcgcc tgctccgcgg cttcctcggg ttcctcgtgg gcctgctcgc cctcaacctc
      661 ctgctcttcc tggtcgtgcc cgagggatgg cgcggggtgg tgctgttcat cgaggccctc
      721 ctcctgatga tcctgatcca cgaggccggc cacttcttca ccgcgaagtg gttcgggatc
      781 aaggtcgagg agttcttcgt cgggttcggc cccaggctgt ggtccaccag gagaggggag
      841 accgagtacg ggatcaaggc catccccgcc ggcggctacg tgcggatcgc gggcatgaac
      901 ccgttccagg agcccgcgcc cgaggaccgc gatcggatct tcggcgccaa gcccgcgtgg
      961 cagcgggcca tcgtcctggc ggcggggtcc ttcacccact tcgtcctggc gatcctcctg
     1021 ctggcctcga tcgtcgcctt cctcggggtg ccggtggagg gtccgccgct cgtggggacg
     1081 gtggaggccc ggctggccgg cgacgtgtcc ccggcagcga gctcggggat ggaggagggc
     1141 gaccagatcg tggaggtgaa cggggagccg atcgactcca acgaggagct cgtcgagatc
     1201 acctcgggga gcgagggcga ggagatggcg atcgtcgtgc tgcgggacgg cgagcgggtc
     1261 acgctccggg ccacgcccgt cctcgacgag atccaggggg agcaggtggc tcgcctcggg
     1321 atccgcatcg agccccctcg cgagaccgag cccgtccacc tggccctgtg gcgcggtgtc
     1381 gaggggacgg gggagatcgc ggtcgaatcc ttcaaggccc tggggcgggt gttcaggccc
     1441 agcggcctgg ccaggatcgg ggagctggtc acggggcagg ccgagcgctc cgtcgaggac
     1501 ccgctcagca tcgtcggagc ggggcgcctc gcgggccagg ccgcctccac cgacaactac
     1561 gacatcctgc tggccttcct cgcggcgctg aacgtgttcg tcgggatcgt caacatcctc
     1621 ccgctgccgc cgctcgacgg cggccacctg gccgtcctgg cctgggagaa ggtcacgggg
     1681 cggaaggtgg atatggcccg actgatcccg ctcaccgccg cggtggccgg cctcctgatc
     1741 ctccttacgt tggccctggc ctacctcgac gtggccaacc ccgtgcccga cccgttccgg
     1801 tagggctcgc ccccagacgg accggcctta aggccaccca aaggggccca cccgacaatg
     1861 ggtccggtcc ggcggctccg tgccgccgcc catggaggtc gggatcatga ggctgatcgg
     1921 cagcgagaga cgactgttgg ggccggctct ggtggtggcg tggacgatgg cgctcgggcc
     1981 ccaggcgtcc ggagccgtgc ccggcgacct ggaccccacg ttcgacggcg acggcatgcg
     2041 ggtcgtggat ctgggaggct cggaggagag ctacgccgtg gccacccgcc ggaacgggaa
     2101 gaccctgatc gccgggacga cgaacgaggg cggcgacaat gacgtcgtgg tcatccagct
     2161 gacgagcggc ggggtcccgg actcgtcgtt cggcggtggg gatggggtcg tcaccacgga
     2221 cttcttcgga ggctatgact cggcctacgc ggtggagctc cttccgaagg gcaagttcct
     2281 cgtggccggg caggctctcg acggccaggg gaagagtcgg tttgccgtgg cgcggttccg
     2341 gcggtcgggg accctcgaca cctcgtacgg cagcaagggg aagcgcacgg tcgccttccc
     2401 gggcaacgcc gtggccctgg acatggtgcg gctgcccggc ggatccgtcg tcctcgtggg
     2461 ggcgagctat ccgaacgaga acttcgtgct ggcacggctc acgaagggcg gcagggtgat
     2521 gaagtcgttc gggaagaagg ggagggtgat caccaacctc gggggcgacg atggggcaca
     2581 cgcgggggcg ctgcaggacg atcgcatcgt ggtggcaggc gggagcggga cgtcgtttgc
     2641 gacgcagaac gtggcgctgg ccaggtaccg gctcggcggc gggctcgaca ggacgttcgc
     2701 cggcaatggg aagaaggtcg tgtcgatggt ggcagcgcgg gactgggcca acgacctcgt
     2761 ggttctcccc aacggccgca tcgtcgtggt cgcgtacgcg agcggtgaca tcgggctggc
     2821 tcggttcaag ccgcgcggcg gcctggaccg aagcttcggc gggggcgacg gcaaggtgct
     2881 cgccgacttc ggggacgacg cgacggggca ggctctgggc cgctccggca agaagctcgt
     2941 cgtggctgga tcgatcgggt tccccccgga tccagcggtc ttccggttcg gccccggcgg
     3001 gggaatcgat gggacgttcg gctcaggggg gatgactatg gccgacttcg gccccgacat
     3061 tgccgccgcc tgggacgtag tcgtccgggg aacgaagatc ctgatcgccg ggtacacgga
     3121 gaccccggga ggcgacatcg tggcggcgcg gttcctcggc tgacgaggcc cctccggccc
     3181 ggctcggagg gggccgtcgg tacactggac ccgtgcagac cgaacgccga gggtcccgga
     3241 aggtcaaggt gggctgggtc gaggtcggag gcgacgctcc cgtccgcgtc cagtcgatga
     3301 cgaccacgaa gacggccgac gtcaacgcga cgctccagca gatggcatcg ctcgtggcgg
     3361 ccggctgcga gatcgtgcgc gtggccgtcc cccacaggga ggacgccgag gcgctgaagc
     3421 agatcgccga gcacgccacg atccccgtga tcgccgacat ccacttccag tggcgctacg
     3481 cgatgatggc cctcgaggcc gggatccacg gcctgcgcat caaccccggc aacatcaagt
     3541 accaggacaa ggtgcgcctg atcgcccgcg aggcccaggc ccgcggcgtc ccgatcagga
     3601 tcggggtcaa cgcggggtcg ctggagaagg acctcctggc gaagcacggt cgccccacgc
     3661 cccaggccct cgtggagtcg gccatgaacg aggtacgcat cctcgaggac gagggcttcg
     3721 ccgacatcaa gatctccgtg aagcactcgg acccgaggga gatgatcgag gcctacaggc
     3781 tgctggcgga gcggtgcgac tacccgctgc acctcggggt gaccgaggcc ggccccatgc
     3841 cggcgggagg cgtgaagtcg gccgtcggca tcgggaccct cctggccgag ggcatcggcg
     3901 acacgatccg cgtctccctc accgacgacc cggtcgagga ggtcaaggtc gggaactgga
     3961 tcctccgctc gctggggctg aaggagctcg gcctcgacct cgtggcctgc ccctcgtgcg
     4021 gacgggccga ggtggacgtc ctggggctga cgaagcaggt caacgaggcg ctggagcgcg
     4081 agggcatcac cgagccgatg cgggtggccg tgatgggctg cgtcgtgaac gggcccggcg
     4141 aggctcgcga ggccgacctg gggatcgcgg ccgggaaggg ctacggccag
//
LOCUS       DASUTW010000068         4158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_797000, whole genome shotgun sequence.
ACCESSION   DASUTW010000068 DASUTW010000000
VERSION     DASUTW010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797000"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VF058_02360"
     CDS             complement(<1..821)
                     /locus_tag="VF058_02360"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6207179.1"
                     /translation="MEVAGMGDVVLLTGGTGFLGTEIARRLLRRPEARIVALVLAPDE
                     DEGLLAAKRAWSYAPDLEEGLAAGRVEIVAGDVRSADLGLEPSVRSGLLRRVTHIVHA
                     AADLRIDVAPADLRPTNVEGVANVLAFARACHADHGLSRLSHVSTAYVAGGRTGEVPE
                     DALDDRFGFENAYELTKYEGERLVRDAMKHLPIFVFRPAMIVGDSRTGEVRTFNSFYV
                     PLRRYLTGHVRVVPASPRLRVNIVPVDHVADAVVRLTFEPAAEGSTFHLTAPAEHL"
     gene            complement(794..2074)
                     /locus_tag="VF058_02365"
     CDS             complement(794..2074)
                     /locus_tag="VF058_02365"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEX6207180.1"
                     /translation="MGRHDQQTLARGIRGNADPRVLLVGYNGANNTGAEALLLVDIAD
                     VQAVLGSGARLTIPALEPRNLARYVGGQPGVDVVPMPMIFPPTVRRLVREHDLIVLVE
                     GATYMDASASALLWYFMWATRCAAVAGKPCLAYAVDAGELKPLNRRLVRRIAPKTDLI
                     VVRSAAAAKRLRSWGVDTRIEVTADNAFRFEPRAEDAQWLEEAWPDAGPHPVGIAAVD
                     YNVWPVVVRPWGRRADCYRWPYYYSRSPKRRAASAALAEGYARLADRIIERHDRSVAL
                     ICMEQLDETLAQRIHERMVHSDRARMFSSRDHDASRMTVLLRSLGALVTSRYHASVLS
                     LPAGVPQVAVRQDLRLQTLYEEMGLADLCVAPGPGMFDTVRFAVDRLLADDGSLGARL
                     RREHQGHLEAARRNPELLAAFVAEHGWRSPAWAM"
     gene            2351..3709
                     /locus_tag="VF058_02370"
     CDS             2351..3709
                     /locus_tag="VF058_02370"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6207181.1"
                     /translation="MVALLDGLGVGIAIALLAWTALLFVPFRWRPVGLYLFMPKVAAG
                     AFTPFIAGAALLLALTGVLRGSWWMAVPAALAAVGASFVIVRLGLVRADLTPALGPGW
                     ADRIPSARRDRMVGRMWRGPLPRAPEPRLRQDVVFATVPGTDRDLLCDVWEPPAGVPP
                     SGLAVVFLHGGAYYILDKDAGTRPLFRHLASQGHVIVDVAYRLFPETDVPGMVGDAAR
                     AVAWTKEHAADLRIRSDRIVLAGGSSGGHLALLAAYAHGDPILTPAALSGSDLDVCGV
                     ASLYGQVDLAAMYEHTSQGKVCRPDDPQPDWDAPPPQWALRVFGEDSVRLRLHHMEAG
                     RCDWLVGGTPTEVPERYEPLSVLTHIHRDLPPTLLVHGLHDEMAPVAEARALHRRLVE
                     AGVPVTAVHLPHTDHMFDLIATRWSPQARAAIHALERFLAALAAGPARAPEPIGAREA
                     PR"
     gene            3730..>4158
                     /locus_tag="VF058_02375"
     CDS             3730..>4158
                     /locus_tag="VF058_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX6207182.1"
                     /translation="MTKSRTVEEMAAPLGSIKSGRLVFTRGASHLELRVDAQMEDLYR
                     ARFEGKVPEIRLTEGTVSVRYRPSIHPPRGEITLSGRIPWALEARWGMSDVTADLQEL
                     ELTSLEISGGASKVEVRMPHPKVAVPIRIGGGASDITVIRP"
BASE COUNT          580 a         1482 c         1488 g          608 t
ORIGIN      
        1 agatgctccg cgggagcggt gaggtggaag gtggaccctt cggcggccgg ctcgaacgtc
       61 agacgcacga cggcgtccgc cacgtggtcg accgggacga tgttgacgcg caggcggggg
      121 ctcgccggca ccacccgcac atgacccgtc aggtagcggc gcagggggac gtagaagctg
      181 ttgaacgtgc ggacctcccc cgttcgggaa tcgcccacga tcatggcggg cctgaagacg
      241 aagatcggca ggtgcttcat cgcgtctcgg accaggcgct ccccttcgta cttcgtcagc
      301 tcgtacgcgt tctcgaagcc gaagcggtcg tcgagcgcgt cctcggggac ctcgccggtc
      361 ctcccgccgg ccacgtaggc cgtcgaaacg tgcgagaggc gcgacagccc atggtccgcg
      421 tggcaggccc gggcgaaggc gagcacgttc gccacgccct cgacgttcgt ggggcggaga
      481 tcagcggggg cgacgtcgat ccgcaggtcg gctgcggcgt ggacgatgtg ggtgacccgt
      541 cggaggagtc cactgcggac gctgggctcg agccccagat cggcggagcg gacgtcgccc
      601 gccacgatct ccacccgacc cgccgccagc ccctcctcga gatccggggc gtaggaccag
      661 gccctcttcg cggccagcag cccctcgtcc tcgtcggggg ccaggaccag ggcgacgatc
      721 ctcgcctcgg gccgccgcag cagcctgcgg gcgatctcgg tccccagaaa ccccgtgccg
      781 ccagtcagca ggactacatc gcccatgccg gcgacctcca cccatgctcg gcgacgaacg
      841 ccgccagcag ctcggggttc cggcgggccg cctcgagatg accttggtgc tctcggcgca
      901 gacgagcacc gagcgacccg tcgtcggcta gcagccgatc gaccgcgaag cggaccgtat
      961 cgaacatgcc ggggccggga gcgacgcaca ggtccgccaa ccccatctcc tcgtacagcg
     1021 tctgcaaccg caggtcctgc cgcaccgcga cctggggcac gccggccggg agcgagagga
     1081 cggaggcatg gtaccgggag gtgacgagcg ctccgaggct ccggaggaga accgtcatcc
     1141 tcgaggcgtc gtggtcgcgg gaggagaaca tccgggcgcg atccgagtgg accatgcgct
     1201 catggatgcg ctgcgccagc gtctcgtcga gctgctccat gcagatcagc gcgaccgagc
     1261 gatcgtgccg ttcgatgatc ctgtcggcga gccgagcata gccctcggcg agggcagcgc
     1321 tcgccgcgcg gcgcttcggg gatcgcgagt agtagtaggg ccagcggtag cagtcggctc
     1381 gtcgacccca cgggcggacg acgacgggcc acacgttgta gtcgacggcc gcgatcccga
     1441 cgggatgggg tccggcgtcc ggccaggcct cctcgagcca ctgcgcgtcc tcggccctcg
     1501 gctcgaaccg gaacgcgttg tccgcggtga cctcgatccg tgtgtcgacg ccccagcttc
     1561 gcagccgctt cgcggccgcc gccgagcgca cgacgatgag gtccgtcttt ggtgcgatgc
     1621 gacgaacgag ccgccgattc agcggcttca gctccccggc atcgaccgcg taggcgaggc
     1681 atggcttccc ggccacggcc gcgcacctcg tcgcccacat gaagtaccag aggagcgccg
     1741 aagcgctcgc gtccatgtac gtggcgccct ccaccaggac gatgagatcg tgttcgcgca
     1801 cgagcctgcg gacggtcggc gggaagatca tcggcatcgg gacgacgtcg acgcccggct
     1861 gcccgccgac gtagcgagcg aggttccgag gctcgagcgc cgggatcgtc agccgggcgc
     1921 cggacccgag caccgcctgg acgtccgcga tgtccacgag gaggagcgcc tcggctcccg
     1981 tgttgttggc gccgttgtag ccgaccagca gcaccctcgg atcggcgttc ccgcggatcc
     2041 cccgcgccag cgtctgctga tcgtgccggc ccatcgctcc acctctcggg tgcagtctcg
     2101 ctcggccgtt cggcccagct tagaggcgat gggcccggca accgtgggtc gctcgggcgg
     2161 tggtcgcggc ctggcgcaga gcggccgtcg ggaggtccgg ctcggggaga aggagtcctc
     2221 cggcccgtga cgagccctgc gttcgaccct aggccgtgag gcccgggagg agcatgctgg
     2281 ggatcgcccg agagatgccg ttcgcctggg cgctggtgcg ccctcggggg gcggggagga
     2341 gggtgcgaga atggtcgccc tgttggacgg gctgggcgtc gggatcgcga tcgccctcct
     2401 ggcctggacc gctctgctct tcgtgccctt ccgctggcgg cctgtgggcc tgtacctctt
     2461 catgcccaag gtcgctgccg gcgccttcac cccgttcatc gccggcgccg cgctcctcct
     2521 cgccctgacg ggggtgctgc gaggatcctg gtggatggcg gtgccggccg cgctcgccgc
     2581 ggtcggggcc tccttcgtga tcgtgcgctt gggcttggtt cgcgccgacc tgacccctgc
     2641 cttgggtccc ggctgggccg atcggatccc ttccgctcgt cgggaccgga tggttggccg
     2701 gatgtggcgt ggaccgttgc cccgcgctcc cgagccccgc cttcggcagg acgtcgtctt
     2761 cgccacggtg cccggcacgg atcgggacct cctctgcgac gtgtgggagc cgccggccgg
     2821 cgtgcccccc tcgggactgg cggtcgtctt cctgcacggc ggcgcgtact acatcctcga
     2881 caaggacgcg ggcacccggc ccctcttccg ccacctggcc tcgcagggcc acgtaatcgt
     2941 ggacgtcgcc taccggctgt tccccgagac ggacgtcccc gggatggtgg gggacgctgc
     3001 gcgggcggtg gcatggacga aggagcacgc cgccgacctg cggatccggt cggaccggat
     3061 cgtgctcgcc ggcggatcgt cgggcggcca cctcgcgctg ttggccgcct acgcgcacgg
     3121 agacccgatc ctcacgccgg cggccctctc cggatccgat ctggacgtct gcggcgtcgc
     3181 gtctctctac gggcaggtgg atctggcggc gatgtacgag cacacctcgc aggggaaggt
     3241 ctgccgcccc gacgatccgc agcccgactg ggacgcaccg ccgccccagt gggcgctccg
     3301 cgtgttcgga gaggactccg tccgtctgcg cctccaccac atggaggccg gccgatgcga
     3361 ctggctggtc ggcgggacgc ccaccgaggt ccccgaacgc tacgagcccc tgtcggtgct
     3421 cacccacatc caccgagacc tcccgcccac gctgctggtg cacggcctcc acgacgagat
     3481 ggcgccggtc gccgaggccc gggcgctgca ccgacgactc gtggaggccg gggtgcccgt
     3541 gaccgccgtc cacctgccac acaccgatca catgttcgac ctgatcgcca cccgctggtc
     3601 cccccaggcc cgggcggcga tccacgcgct ggagcgcttc ctggcggccc tggccgccgg
     3661 cccggcgcgg gcgccggagc ccatcggggc ccgtgaggcc ccgaggtgac gtgaaaggag
     3721 atggagaaga tgacgaagag cagaaccgtc gaggagatgg ccgccccgct gggctcgatc
     3781 aaatcgggtc gcctcgtgtt cacgcggggc gcttcacacc tggagctgag ggtggacgct
     3841 cagatggagg atctctaccg agcccggttc gaggggaagg tgcccgagat ccggctcacc
     3901 gaggggaccg tgtccgttcg gtaccgtccc agcatccatc cgccccgcgg cgagatcacg
     3961 ctctccggga ggatcccgtg ggcgctcgag gccaggtggg ggatgtcgga cgtcaccgcc
     4021 gacctgcagg agctcgagct cacgagcctg gagatctcag gaggagcctc gaaggtcgag
     4081 gtgcggatgc ctcacccgaa ggtcgccgtc ccgatccgga tcggcggtgg cgccagcgac
     4141 atcacggtga tccggccg
//
LOCUS       DASUTW010000069         3154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_538830, whole genome shotgun sequence.
ACCESSION   DASUTW010000069 DASUTW010000000
VERSION     DASUTW010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538830"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /gene="ilvE"
                     /locus_tag="VF058_02380"
     CDS             complement(<1..561)
                     /gene="ilvE"
                     /locus_tag="VF058_02380"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEX6207183.1"
                     /translation="MPITPVEKVWMDGELIDWDDARIHVLTHALHYGTGVFEGIRAYS
                     TKQGPGIFRLTDHIRRLTHSASVYLMEVPYTPEELIEACKEVVRANDLDACYVRPVVY
                     RGYGEMGLNPLNAPVNTFIVTWRWGTYLGDEALQNGARVMISSWRRIDSNALPSGAKG
                     TGQYVNSSLAKIEALKAGYDDAIMLSP"
     gene            complement(623..2200)
                     /gene="serA"
                     /locus_tag="VF058_02385"
     CDS             complement(623..2200)
                     /gene="serA"
                     /locus_tag="VF058_02385"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX6207184.1"
                     /translation="MRVLVTETLAEQGLSLLRESFEVDVRIELASEGLASAIAPYDAL
                     IVRSQTKVDAAVLEAAERLKVVGRAGVGLDNVDVEAATRRGVMVVNAPQSNVLSAAEH
                     TIALLLAQARNIPQAHAALRAGDWDRRRFQGTELNGKTLGILGLGRVGTLVAQRALAF
                     GMRLIAFDPFVPKDRARQLGIELMPDLGALLVQADVVTIHLPRIPETEGLLGERELRM
                     MKAGARLVNTARGGIVDEAALAAVLESGHLAGAALDVFDEEPVTSHPLFRLENVVVTP
                     HLGAATKEAQDKAGTSIAEMVKLALEGEFVPYAVNVAAAEVSELTRPFLPLAERLGKV
                     LQGLAEEAVGAVHVEYQGRVAEHDTSVLTLSVLKGLLSGVVHEPVSFVNAPIIARDRG
                     IQISDTRRLEAEDYVSLVAVSAETEAGPVQVAGTLAGPRGAERIVRVYDFDDVEMAPA
                     EHMAFFLYEDRPGVIGTVGTILGDAGINIAGMEVGRKEAGGLALMGLAVDAPIPEDVL
                     ERIVREAKMQRARSIRL"
     gene            complement(2197..>3154)
                     /locus_tag="VF058_02390"
     CDS             complement(2197..>3154)
                     /locus_tag="VF058_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX6207185.1"
                     /translation="GGLESSVANLFSPGDRVVVPVTGYFGDRFARIAEAFHLDVRRVE
                     YDWGRAVDVADVEEALGEAPTKGLLFQHSETSTGVINDVEPIARAAKEAGALTALDVI
                     SSAGAVPLETDAWGIDVAIGGSQKALSATPGIAFASISEAAWAAHRESTNPRFYFDWT
                     ADRKAYERENPEAPWTPAISLMLGLREALRLYLEEGAEAVLRRHELLSTATKEGVRAL
                     GLDLFGEHPERAWAVTTVRVPDGVDGKAIPAKMRADHGIIIAGGQGPLAGRVFRIGHL
                     GYYDRFDIVRCLAALELTLMDLGYPAKPGSAVAAAEEVFARA"
BASE COUNT          439 a         1163 c         1098 g          454 t
ORIGIN      
        1 cggcgagagc atgatcgcgt cgtcgtaccc ggccttcagc gcctcgatct tggccagcga
       61 ggagttcacg tactgccccg tgcccttcgc gcccgacggg agggcgttcg agtcgatccg
      121 tcgccaggac gagatcatga cccgggcgcc gttctggagg gcctcgtccc ccaggtaggt
      181 cccccagcgc caggtgacga tgaaggtgtt gaccggagcg ttcagcgggt tgagccccat
      241 ctccccgtag ccgcggtaga cgaccgggcg gacgtagcag gcgtcgaggt cgttggcccg
      301 gacgacctct ttgcacgcct cgatcagctc ctcgggcgtg tacggcacct ccatcaggta
      361 gacggaggcc gagtgcgtga ggcggcggat gtggtcggtg aggcggaaga tgccgggtcc
      421 ctgcttcgtg gagtaagcgc ggatcccctc gaagacgccg gtgccgtagt ggagggcgtg
      481 ggtcagaacg tggatcctgg cgtcgtccca gtcgatgagc tccccgtcca tccagacctt
      541 ctcgaccggc gtgatgggca tccgcgtcct cctggctccg ggtgccctca gggtacatgg
      601 cgccccgggg gcccgaccac cgtcagaggc ggatcgatcg ggcccgctgc atcttcgcct
      661 cgcggacgat gcgctccagc acgtcctcgg ggatcggggc gtccacggcg agccccatca
      721 gcgccaggcc ccccgcctcc ttccgaccca cctccatccc ggcgatgttg atcccggcgt
      781 ccccgaggat cgtccccacc gtgccgatca cgcccgggcg gtcctcgtac aggaagaacg
      841 ccatgtgctc ggccggcgcc atctccacgt cgtcgaaatc gtagacgcgg acgatccgct
      901 cggcgccccg gggccccgcc agggtgccgg ccacctggac cgggcccgcc tcggtctcgg
      961 cggagacggc gacgaggctg acgtagtcct cggcctcgag gcggcgggtg tcgctgatct
     1021 ggatgccgcg atcccgagcg atgatcggcg cgttgacgaa cgagaccggc tcgtggacga
     1081 cgccggacag cagccccttc agcacggaga gcgtcagcac gctcgtgtcg tgctccgcca
     1141 cccgcccctg gtactcgacg tggacggcac cgaccgcctc ctcggccagg ccctggagga
     1201 ccttccccag ccgctcggcc agcgggagga aggggcgggt cagctcggag acctcggccg
     1261 cggccacgtt gaccgcgtag gggacgaact cgccctccag cgccagcttc accatctcgg
     1321 cgatcgaggt ccccgccttg tcctgggcct ccttggtcgc ggcgcccagg tgcggcgtca
     1381 ccacgacgtt ctccagccgg aagagcggat gggaggtgac cggctcctcg tcgaagacgt
     1441 cgagcgccgc gcccgccaga tggccgctct cgaggaccgc cgcgagcgcg gcctcgtcca
     1501 cgatcccgcc ccgggcagtg ttgacgagcc gggcgcccgc cttcatcatc cgcagctcgc
     1561 gctcccccag cagcccctcg gtctcgggga tccgcgggag gtggatcgtg acgacgtcgg
     1621 cctgcaccag gagcgcgccg aggtccggca tcagctcgat ccccagctgc cgagcccgat
     1681 ccttgggcac gaacggatcg aacgcgatca gccgcatgcc gaaggccagg gcgcgctggg
     1741 cgacgagggt ccccacgcgt ccgagcccca ggatccccag cgtcttgccg ttcagctccg
     1801 tcccctggaa ccgcctgcgg tcccagtcgc ccgcgcggag cgccgcgtgc gcctggggga
     1861 tgttccgggc ctgcgccagc agcagcgcga tcgtgtgctc ggcggccgag agcacgttcg
     1921 actgcggtgc gttgaccacc atgacgccgc gcctggtggc tgcctcgaca tccacgttgt
     1981 cgaggcccac gccggcgcgg cccacgacct tcaggcgctc ggcggcctcc aggacggccg
     2041 cgtcgacctt ggtctgggac cggacgatga gcgcgtcgta gggggcgatc gcggatgcga
     2101 gcccctcgga ggcgagctcg atgcggacgt cgacctcgaa cgactcgcgg aggagcgaca
     2161 gcccctgctc ggccagcgtc tcggtgacga ggaccctcac gcccgggcga agacctcctc
     2221 ggccgcggcc accgccgacc ccggcttggc cgggtacccg aggtccatga gcgtgagctc
     2281 cagtgcggcc aggcaccgca cgatgtcgaa gcggtcgtag tagccgaggt ggccgatccg
     2341 gaacaccctg ccggccagcg gcccctggcc gcccgcgatg atgatcccgt ggtcggcccg
     2401 catcttcgcg gggatcgcct tcccgtccac gccgtccggc acccggaccg tggtgaccgc
     2461 ccaggcccgc tccgggtgct cgccgaacag gtccaggccg agggcccgca cgccctcctt
     2521 cgtcgccgtc gagagcagct cgtggcggcg gaggaccgcc tcggccccct cctcgaggta
     2581 cagccgcagc gcctcgcgca ggccgagcat cagcgagatg gccggggtcc acggagcctc
     2641 ggggttctcc cgctcgtagg ccttccggtc cgcggtccag tcgaagtaga agcgcgggtt
     2701 cgtcgattcg cggtgggcgg cccaggcggc ttcggagatc gaggcgaagg cgatccccgg
     2761 cgtcgccgag agcgccttct gcgagccgcc gatcgccacg tcgatgcccc aggcgtcggt
     2821 ctcgagcggc acggccccgg cgctggagat cacgtcgagc gcggtcaggg ccccggcctc
     2881 cttcgcggcc ctggcgatcg gctccacgtc gttgatcacg cccgtcgagg tctcggagtg
     2941 ctggaacagc aggcccttcg tgggggcctc gcccagcgcc tcttccacgt cggcgacgtc
     3001 gacggcgcgg ccccagtcgt actcgacccg acggacgtcg aggtggaagg cctcggcgat
     3061 cctggcgaag cgatccccga agtaaccggt caccgggacg acgacgcggt cgcccggcga
     3121 gaagaggttg gccaccgagg actcgagccc tccc
//
LOCUS       DASUTW010000070         2884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1186936, whole genome shotgun sequence.
ACCESSION   DASUTW010000070 DASUTW010000000
VERSION     DASUTW010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186936"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1692)
                     /locus_tag="VF058_02395"
     CDS             complement(<1..1692)
                     /locus_tag="VF058_02395"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207186.1"
                     /translation="MRGSIAMAVAAVLILAAAVRAQQPVIPPGYTAYRISGPGISGVP
                     PLPFRIEGDRLVAPEGSLVSVTASALTADRVRLSLVTPDGPATFVGLRDGEAFAGFLL
                     QEGEQRWAMPMTFVPAGQETALAPDTGLLGSGEGVSRVEIVPANVTLRSNEQRAFVAR
                     VYDESGAEVADADVEWFAFGGTITEEGDFHALNAGERQVVALVNGTAIGTTTVIVEEP
                     AITSLTVWPEVPSRLAVGSRVVLEVDALTAIGRWDLDPAVVLESSAPSVVGVEGWTLA
                     ALSPGRAQVTLSAGEARESWSIEVVAASGALSITGVPTGAVRTGDVVRLGTTVADAVP
                     LWAVAEAGAEVWPDGAFVAERAGTYTVLATLGDEVAEARVAVESRGLSGRIRIQGHGT
                     NVGTYTSDLWPQSGYVYVGTHQANQLRTYDVSDPTSPVLTDSQAFDARVVNDVKVSED
                     GKWLVATREGASDRRNGILVFSLSDPSHPELVSEYTETLTSGVHNVFWVGSLVYAVND
                     GTGDLHIIDVSDPASPREVGRWGLPVEGRSLHDVWVQEGVAYLSYLRDGLVIVDVG"
     gene            1882..2706
                     /gene="mtgA"
                     /locus_tag="VF058_02400"
     CDS             1882..2706
                     /gene="mtgA"
                     /locus_tag="VF058_02400"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEX6207187.1"
                     /translation="MRPAARALAFVVVSAAALVSASVLMYFPVVVWHRYRPPEVTSVM
                     RYRAAEARASGEPWRLRYDWVPLDRISPHMERAVLAAEDSRFYEHRGFDLEQIQAAWE
                     RRERGGELRGASTITQQTVKNLYLTPRRSLLRKIREAALTAWMELWLPKRRILELYLN
                     IVELGPGVFGVEAAARGYFDRPAARLTPSQAALLAATLPAPLLRNPARPTPGLRRLQA
                     LVLRRMERWYGSGERIEPPSPAAAPARRPSVPLELPAEATEAPLAPADTPVEGEVP"
     gene            2703..>2884
                     /locus_tag="VF058_02405"
     CDS             2703..>2884
                     /locus_tag="VF058_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207188.1"
                     /translation="MRSDRLVGARVLILVSFVTLTLGFVAGYWMKGAGLERGRDAGAP
                     HAVGVEEYVRLGMSSLA"
BASE COUNT          442 a         1106 c          938 g          398 t
ORIGIN      
        1 ccccacgtcc acgatcacca gaccgtctct cagatacgac aggtacgcca ctccctcctg
       61 cacccacacg tcgtgcaggc tgcggccctc caccggaagg ccccagcgac ccacctcgcg
      121 ggggctcgcc gggtccgaga catcgatgat gtgcaggtcc cccgtcccgt cgttcaccgc
      181 gtacaccaac gagcccaccc agaacacgtt gtggacaccc gacgtcaacg tctccgtgta
      241 ctccgagacc agctcggggt gcgacggatc cgacaggctg aacaccagga tcccgttccg
      301 acggtcggat gctccctcgc gggtcgccac cagccacttc ccgtcttccg acaccttgac
      361 atcgttcacc acccgcgcgt cgaacgcctg cgagtccgtc aacaccggag acgtcgggtc
      421 gctcacgtcg tacgtccgca gctggttcgc ctggtgcgta cccacgtaca cgtaccccga
      481 ctgcggccac aggtccgacg tgtacgtacc cacgttcgta ccgtggccct ggatacggat
      541 ccggcccgac aaaccacgcg actccaccgc cacacgcgcc tccgccacct cgtcccccaa
      601 ggtcgccagc accgtgtacg tccccgcgcg ctcggccacg aacgcaccgt ccggccatac
      661 ctccgcaccc gcctccgcca ccgcccacag cggtaccgcg tccgccaccg tcgtccccag
      721 acgcaccacg tcacccgtcc ggaccgcgcc cgtcggcaca cccgtgatcg acagcgcccc
      781 cgacgccgcc accacctcga tcgaccagct ctcacgggcc tccccagccg acagcgtcac
      841 ctgcgcccga cccggcgaca gtgccgccag cgtccaaccc tccacaccca ccaccgaagg
      901 cgcgctcgac tccagcacca ccgccggatc cagatcccag cgaccgatcg ccgtcagcgc
      961 gtccacctcc aaaaccacgc ggctccccac cgccagacgc gacggtacct cgggccacac
     1021 cgtcagcgac gtgatcgccg gctcctcgac gatcaccgtc gtcgttccga tggcggttcc
     1081 gttcaccagc gccacgacct gacgctcgcc cgcgttaagg gcatggaaat cgccctcctc
     1141 cgtgatcgtc ccgccgaacg cgaaccactc gacgtcggcg tcggcgacct cggcgcccga
     1201 ttcgtcgtac acgcgcgcca cgaacgcccg ctgctcgttg gacctcaggg tcacgttcgc
     1261 ggggacgatc tcgacacggg acaccccctc gcccgagccc aggagcccgg tgtcgggcgc
     1321 gagcgccgtc tcctggcccg cggggacgaa ggtcatgggc atggcccagc gctgctcgcc
     1381 ctcctggagg aggaagccgg cgaacgcctc gccgtcgcgt agcccgacga aggtcgcggg
     1441 accgtccggg gtcacgaggg acaggcggac gcggtcggcg gtcagagcgc tcgcggtgac
     1501 ggacacgagg ctcccctcgg gggccaccag gcggtcgccc tcgatgcgga aggggagcgg
     1561 cgggacgcca ctgatgccgg ggcccgaaat ccggtaggcg gtgtagcccg gaggaatcac
     1621 cggttgctga gcccgaacgg cagcagctag gatgaggacg gcggcgacgg ccattgcgat
     1681 cgagccacgc atgcgcttga gctcctgtcg ttgcacgaag agggactcca gatggaagtg
     1741 gggatctgag tacaagagct gaacgtcggc ccgccgggcg aggtttcagg atgacggttc
     1801 ccgcggcggg gacgaggccc cgaagataca tgccccgtac cgcccgatca ccgtcgggcc
     1861 gcgcgcgggc tcggtgaggc tttgcggccc gctgcccgag cgctggcctt cgtcgtcgtg
     1921 tcggcggccg cgctcgtgtc cgcctccgtt ctcatgtact ttccggtcgt cgtctggcac
     1981 cgctaccggc ctcccgaggt cacgtcggtg atgcgctatc gcgcggccga ggcgcgcgct
     2041 tcgggagagc cgtggcgcct acgctacgac tgggtgcccc tcgaccggat ctcgccgcac
     2101 atggaacggg cggtgctggc agcggaggac tcccgcttct acgagcatcg cggcttcgac
     2161 ctcgagcaga tccaggccgc ctgggagaga cgcgagcggg gcggagagct gcggggcgct
     2221 tccacgatca cccagcagac ggtgaagaat ctgtatctga cgccgcgacg aagcctcctc
     2281 cggaagatcc gcgaggccgc cctgaccgcc tggatggagc tctggctccc gaagcgcagg
     2341 atcctggagc tctacctcaa catcgtcgag ctgggcccgg gggtcttcgg cgtcgaggcc
     2401 gccgcccggg gctacttcga ccggccggcg gcgcggctca cgccgtccca ggcggcgctc
     2461 ctggcggcca ccctgccggc gccgctcctt cgcaacccgg cgcgaccgac gccgggcctc
     2521 cggcgcctgc aggccctggt cctgcggagg atggagcgct ggtacggatc gggggagagg
     2581 atcgagccgc cctctccggc agccgcgccc gcgcgcaggc cgagcgtccc tctcgagctg
     2641 cccgcggagg ccaccgaggc accgctcgcc cctgccgata cgcctgtcga aggcgaggtc
     2701 ccgtgaggag cgaccggctg gtgggggcgc gggtcctgat cctcgtctcc ttcgtgacgc
     2761 tgaccctcgg gttcgtggcc ggctactgga tgaagggagc cgggctggag cggggccggg
     2821 acgcgggggc gccgcacgcc gtcggggtcg aggagtacgt gcggctgggg atgagctcgc
     2881 tggc
//
LOCUS       DASUTW010000071        11119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_280170, whole genome shotgun sequence.
ACCESSION   DASUTW010000071 DASUTW010000000
VERSION     DASUTW010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11119
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280170"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VF058_02410"
     CDS             complement(<1..228)
                     /locus_tag="VF058_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSLREA domain-containing protein"
                     /protein_id="HEX6207189.1"
                     /translation="MVAALAGAPPASAATTLVVDTTTDGYGNGPGCTLREAIVSSNVD
                     LPIDYCDGGSDPTTIRVPAGRFRLSVGPAGDN"
     gene            complement(375..926)
                     /locus_tag="VF058_02415"
     CDS             complement(375..926)
                     /locus_tag="VF058_02415"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX6207190.1"
                     /translation="MRIYTKAGDDGTTGLLYGGRIPKSDPAAEAYGTVDEAVACLGLA
                     RANATDPAAHRLILEIQRDLFIVGADLATNPEERGKLKPGVSLTTSEMIERLEGTIDA
                     LVEVSPIPEHFVVPGASPGSAALDIARAVVRRAERRAVALREAGREVNPEAIRYLNRL
                     SDLLFVLARSQAGGPEEPSRSEA"
     gene            1232..2110
                     /gene="dapA"
                     /locus_tag="VF058_02420"
     CDS             1232..2110
                     /gene="dapA"
                     /locus_tag="VF058_02420"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX6207191.1"
                     /translation="MAGSFGTLVTAMVTPFKDDGSLDLDRARDLAAWLFDHGTDALVV
                     TGSTGEAATLSDGEKVDLWRACAEVGGGTIIAGTGTYDTAHSVHLTHQAEEAGVDAML
                     VVTPYYNKPPQRGLIEHFTTVAGATSKPVMVYNIPGRTGTRIEHDTLLRLVEVENIVA
                     VKDSTGDLDGVSHLVAQAPTDFELYCGDDWATFAMMCAGARGLVSVAAHVVGERLKRM
                     MELIEGGDAAGARKIHEELLPVFDALFITSNPIPLKAAMEMIGQPVGSPRLPLVPATE
                     DERSRIRKALEDAGVL"
     gene            2094..3752
                     /locus_tag="VF058_02425"
     CDS             2094..3752
                     /locus_tag="VF058_02425"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX6207192.1"
                     /translation="MQASSSLVFLGGVGEVGRNMACLESNGRVLVLDAGLSFPTEEMP
                     GIDLVLPDWEWLRRRRDRIDGLILTHGHEDHMGAVPYLLREIPLRIHATALALALLRP
                     KLEEHGVEDRAELVEVHPGREARIGSFTARFHRVTHSIPDGVAVAIDTPQGTILHSGD
                     FKLDPTPIDERPTDLQGIADEARRGVHVFLSDSTNAEEKGTIPSERTVGPRLLEIFRR
                     GERLIVAACFASHIHRVQQICDAAREVGRKIAFLGRSMHQSVEQGRRLGYLDVPEEDV
                     IDIERLKDHDPREVAVVCTGSQGEPLSALALMAAREHKWVDLEEGDLVVIAASFIPGN
                     ETAIHRTIDGLYRTGADVFHSKIANVHVSGHAAADELQFMLSLVRPRWFIPIHGERRH
                     LAHHARLARAVGIPGDRILIVEDGDVVEVGAEAVKIVDRAPSGMIFVDGLGIGDVGDV
                     VLRDRRKLAGDGFVVVVVTADAHSGEVVAGPDVITRGFVHDETSAEMLEEAKARTLAS
                     LAEVAEEEVADPSILRQHIRRTLGRYFFEAIRRKPVIVPVIIEV"
     gene            3752..5908
                     /locus_tag="VF058_02430"
     CDS             3752..5908
                     /locus_tag="VF058_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX6207193.1"
                     /translation="MAKKRKKRRTGGAVSAAGQKVAEHLGPQLTDALGIGLLVLAGLS
                     VLGIWLDAAGPLGRFLTIVVVGMLGLAGYAFPVLALYWGLLLVRGTAEEDRGRMFIGL
                     IFLLMGGLGIVSLVRGNPSPAAGFSGFGDAGGLVGALTAWPLSKVMSVYGAAIVCIGL
                     VALGALVFTATPLRSVWEWLVRPKDEVLDLTEAEEPAPRRRRRKEPEVEGEILTPTLP
                     DPEPQEEPTPSVVPSPAGRGYKLPPVDMLRKAPASGGPGRDEKETMAILERTLRNFGV
                     AASVVGASRGPTVTLFEVEVSAGTKVNKVLNLGDDIAYALATPDVRIIAPIPGRSAIG
                     VEVPNKVRDFVTLGDVLGSKAARREKHRLAVALGKDVQGNPLLLRLNEMPHLLIAGAT
                     GAGKSSLINAFVTSILVRAHPDEVKLMLVDPKRVELSHFADLPHLISPVIVAPKRAAE
                     ALGWVVREMELRYESLATVGMRDIDSYNGAHADGSLRIPPGRDAEFERLPYILVVIDE
                     LSDLMMVAPRDVEDAIVRISQMARAVGIHLVVATQRPSVDVVTGLIKANIPSRIAFMT
                     ASAADSRVILDVGGAEKLVGHGDMLFLPSSASKAARIQGAWVTQHEIDAVADYVRQQR
                     EPDYVEGIEGLGKPPADDEDLDAEDDLLQQAMELVIHSQLGSTSMLQRKLKVGFARAG
                     RLMDMLEERGVVGPSVGSKPRDVLITWDEYAESRTG"
     gene            complement(5924..6247)
                     /locus_tag="VF058_02435"
     CDS             complement(5924..6247)
                     /locus_tag="VF058_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6207194.1"
                     /translation="MTTRLEARVEAGHRIRLAGELDISTVPVLEQSVDGLVDDPLILE
                     LTDLTFLDSTGLRAILRLARSHADRGRLVLRAPTPRVLEVLTICGVLGGPLNLELERK
                     AEAAG"
     gene            complement(6244..6681)
                     /locus_tag="VF058_02440"
     CDS             complement(6244..6681)
                     /locus_tag="VF058_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6207195.1"
                     /translation="MKAPRHRSIRPGSSAGRLGRSPPSIAAAIRCGARRSPDPRRLSI
                     EPLGRRALALIGEIDTSVRSKVASAIADVVSSPGGAVLDLAGLRFMDSAGIHVLTEAA
                     RELKGDDAITVRNPRPCVLRVLVAAATLGWEGRLRIELDRTDP"
     gene            complement(6678..7028)
                     /locus_tag="VF058_02445"
     CDS             complement(6678..7028)
                     /locus_tag="VF058_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207196.1"
                     /translation="MVTSWRDVEVSNGTVFRRMNEWTHDDTEHLGNRIEAYLCECGDT
                     SCTDPITIHRVKYERVRAYPARFVIALDHENPEIDYVVREAPGFAVVEMVGGSARIAR
                     ATDPRRSHPTGVDR"
     gene            complement(7028..7405)
                     /locus_tag="VF058_02450"
     CDS             complement(7028..7405)
                     /locus_tag="VF058_02450"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6207197.1"
                     /translation="MPEASPTAEPLGVRIQRRNGVVRVLLRGALDLAGARRLAEELES
                     VSSDGAEGVLLDARDVTFADPTGLRAMFDALLHMRSEGARTAVIGVTPAVRRLLELTG
                     TGSALDGAAGFGLLRRFSSEEGG"
     gene            7577..8200
                     /locus_tag="VF058_02455"
     CDS             7577..8200
                     /locus_tag="VF058_02455"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEX6207198.1"
                     /translation="MEPPKQATSLRILIADGDVAHLEAIAELVTALGHEVVARETSLE
                     ELGAHTVAEQPDVALVLVEEGPTALDRIDEIVQEAVCPVIAVLRGEDPAFINEAARRG
                     ISAYVAADDPEEMQSSIDIVLRRFGEYRGLQDAFVRRAVTERAKGILMERHGVDEHAA
                     FLMLRDEARSTRRKLVELAEAVVSGHRLLTADRRPDRTPSGPEEPGD"
     gene            8270..8485
                     /locus_tag="VF058_02460"
     CDS             8270..8485
                     /locus_tag="VF058_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207199.1"
                     /translation="MDELRDEDIRTEDQESGGAPAPERDADEQDADGVDQADGQDQGD
                     EVDDADQGDQGDHDDDATDEDADQEDA"
     gene            8611..9672
                     /locus_tag="VF058_02465"
     CDS             8611..9672
                     /locus_tag="VF058_02465"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6207200.1"
                     /translation="MDRLVTGGLMRLPGMRLVRDGATVPVGDYTRTIRIGSRRVPETV
                     RPERVVHHFARGATIVLQALHRQWPPIAAFCRRLELDLTHPVQANAYVTPATSRGFAV
                     HHDTHDVFVIQTHGRKGWRVYLPVIELAGKEQPWSSELGDPGAPILETELSPGDVLYI
                     PRGFPHDATAREEVSIHVTVGIQARTWLDVWRHVMEQAPEHAPFREALPIGFARDREA
                     LAGEVAARLPELRSWLEKAAGPEAAASFAEGFWDGRPPVLGGQLEQVAQLDAVDVTTP
                     LRVRAGAIFRPGVEGEEAVARLGTRILRMPGWVEPALRFVAEAVGPFTPRELPNLNPG
                     SALVLCRRLIVEGVLEADG"
     gene            9665..10546
                     /locus_tag="VF058_02470"
     CDS             9665..10546
                     /locus_tag="VF058_02470"
                     /inference="COORDINATES: protein motif:HMM:NF018677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HEX6207201.1"
                     /translation="MADAPTCSALALAMDEPLQATASRIRHWLLVEQPGRWGHHALLD
                     SELPREVGEHLAIEGHHHGVRVLLIKRRSRGPGGSRRCFAAFTGRRERRLITFQIDAP
                     RDLLDLDLGALARDRWSGLGEEIAGPLFAVCTHGKHDQCCARLGAPVLRALSHREDTW
                     EVTHVGGDRFAGNLLAFPHGLYFGRVTGEAAGRVADAYAGGRIALDHFRGRSAFTPAA
                     QAAEHHLRAALGVVGVDDVVLTGHRSLGPLHRVDFTVRGEARVVEVEEVLLEPRPLTC
                     KSGAPRAPRSFVLRGLS"
     gene            complement(10548..11036)
                     /locus_tag="VF058_02475"
     CDS             complement(10548..11036)
                     /locus_tag="VF058_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX6207202.1"
                     /translation="MGDETVLVVVEIPRGSRNKYEVDHETGHLVLDRMLFTSMVYPAD
                     YGFIEGTLGSDGDPLDALVFVGEPTFPGCRIRVRPVGLFRMWDEKGADEKILCVPLSD
                     PMWSHVQDLEDVREPLRNEIEHFFAVYKDLEDKPSGVEGFEDREAALRVIAEARERAR
                     GA"
BASE COUNT         1582 a         3874 c         4068 g         1595 t
ORIGIN      
        1 gttgtcgccg gcggggccca ccgacagcct gaagcgcccg gccgggacgc ggatcgtcgt
       61 ggggtcgctt cctccgtcgc agtagtcgat cgggaggtcc acgttcgacg acacgatcgc
      121 ctctcgcagc gtgcagccgg ggccgttccc gtagccgtcg gtggtggtgt cgacgacgag
      181 cgtggtcgcg gcgctcgcgg gcggcgcgcc cgcgagcgcc gcgaccaacg tcaggacgat
      241 caagatcggc gcgcggggaa gcccttcccc gacgcgccca cgtggtgccg acaagcgaac
      301 ggacatccgg gacctccctt cagcgtgccg ggagcatatc ccgcgtgtct tcgagtgcgg
      361 ttaagggtcc gccgtcaggc ctcgctccgg ctgggctctt ccggcccccc cgcctgggag
      421 cgggccagga cgaacagcag gtccgagagc cggttgaggt agcggatcgc ctcggggttg
      481 acctcacgtc ccgcttccct gagggccacg gctctcctct cggcccgtct caccacggcc
      541 cgcgcgatgt cgagcgccgc ggagccggga ctcgctccgg gcaccacgaa gtgctcgggg
      601 atcggcgaga cctcgaccag ggcgtcgatc gtcccctcca ggcgctcgat catctccgag
      661 gtcgtcagcg agacgccggg cttgagcttc ccccgctcct cggggttcgt ggccaggtcc
      721 gctcccacga tgaagaggtc ccgctggatc tcgaggatca gcctgtgggc ggccgggtcg
      781 gtggcgttcg ccctggcgag ccccaggcac gccaccgcct cgtccaccgt gccgtacgct
      841 tcggcggcgg ggtcggactt cgggatgcgg cccccgtaca gcagcccggt ggtgccgtcg
      901 tcgccggcct tcgtgtagat ccgcatcgcc catagcatac gggggtccag gcccgggagc
      961 cggtcggcgg gggtctcccg tggctccgga acccctcgag gccggggcat cactcgttcc
     1021 ccgtgcggca cgagtggcac atgaggctct tgttgttcag gtcaaccgct gttagcatga
     1081 ccccgcgtgg gggcgggccg accgggcaag gcgggacgcc cgactggcgc ccggtcggtc
     1141 cccgcgcaac cccgggtcgt gcgacgatgg gatcgccccg ccgcaccccg ccggaggggc
     1201 ggacgccgcc gcagcgggaa ggaggcagca gatggccggc agcttcggaa ccctcgtcac
     1261 cgccatggtg accccgttca aggacgacgg atcactcgac ctcgaccggg cccgcgacct
     1321 cgccgcctgg ctcttcgacc acggcacgga cgcgctggtc gtcacagggt ccacgggaga
     1381 ggcggcgacg ctctccgacg gggagaaggt ggacctctgg cgggcgtgcg ccgaggtcgg
     1441 cggcggcacg atcatcgccg ggaccggcac ctacgacacc gcgcactcgg tccacctgac
     1501 ccatcaggcc gaggaggcgg gcgtggacgc gatgctcgtc gtcaccccct actacaacaa
     1561 gcctcctcag cgcggcctga tcgagcactt cacgaccgtg gccggggcca cctcgaagcc
     1621 cgtcatggtc tacaacatcc ccggccgcac gggcacgcgc atcgagcacg acaccctgct
     1681 gcggttggtc gaggtggaga acatcgtcgc ggtgaaggac tcgaccggcg acctcgacgg
     1741 cgtctcccac ctcgtcgctc aggccccgac cgacttcgag ctctactgcg gcgacgactg
     1801 ggccaccttc gccatgatgt gcgccggcgc ccgcggcctc gtctcggtcg ccgcccacgt
     1861 cgtgggggag cggcttaagc ggatgatgga gctgatcgag ggcggggacg cggcaggagc
     1921 gcggaagatc cacgaggagc tgctgccggt gttcgacgcg ctgttcatca cgagcaaccc
     1981 gatcccgctg aaggccgcga tggagatgat cggccagccc gtcgggtcgc cgcgcctgcc
     2041 gctcgtcccg gccacggaag acgagcgctc ccggatccgc aaggcgttgg aggatgcagg
     2101 cgtcctctag cctcgtcttc ctcggcggcg tgggggaggt ggggcgcaac atggcctgcc
     2161 tcgagtcgaa cggccgcgtc ctcgtgctcg atgcgggcct ctcgttcccc accgaggaga
     2221 tgcccgggat cgacctcgtc ctccctgact gggagtggct gcggcggcgg cgggaccgga
     2281 tcgacggcct gatcctcacc catggccacg aggaccacat gggggcggtg ccctacctcc
     2341 ttcgggagat cccgctgagg atccacgcca cggcgctggc gctcgccctc ctccgcccca
     2401 agctggagga gcacggcgtc gaggaccggg ccgagctcgt cgaggtgcac ccgggtcggg
     2461 aggcgcggat cgggagcttc accgcgcggt tccaccgcgt cacgcactcg atccccgacg
     2521 gcgtggccgt ggcgatcgac acccctcagg ggacgatcct ccattcgggg gacttcaagc
     2581 tggatcccac gccgatcgac gagcgtccga ccgacctgca ggggatcgcg gacgaggcga
     2641 ggcgaggcgt ccacgtcttc ctctcggatt ccacgaacgc cgaggagaag gggacgatcc
     2701 ccagcgagcg aacggtcggc ccgcggctcc tcgagatctt ccggcggggc gagcggctga
     2761 tcgtggcggc gtgcttcgcc agccacatcc acagggtcca gcagatctgc gatgccgcgc
     2821 gcgaggtggg gcggaagatc gcctttctcg gacggtcgat gcaccagtcc gtggagcagg
     2881 gccgccgcct cggctacctc gacgttccgg aggaggacgt gatcgacatc gagcggctca
     2941 aggaccacga tccccgcgag gtggccgtgg tctgcacggg gagccagggc gagccgctgt
     3001 cggcgctcgc cctgatggcg gcccgcgagc acaagtgggt ggacctcgag gagggtgacc
     3061 tcgtggtgat cgcggcgtcg ttcatccccg ggaacgagac cgcgatccac cgcacgatcg
     3121 acgggctgta ccggacgggg gccgacgtct tccactcgaa gatcgcgaac gtccatgtgt
     3181 cgggccacgc ggccgcggac gagctgcagt tcatgctctc gctcgtccgc ccccgctggt
     3241 tcatcccgat ccacggggag cggcgccacc tggcccacca cgctcggctt gcgcgcgccg
     3301 tcgggatccc gggagaccgc atcctcatcg tggaggacgg cgacgtggtg gaggtggggg
     3361 ccgaggcggt gaagatcgtc gaccgggccc cctcggggat gatcttcgtg gatggcctcg
     3421 ggatcggcga cgtgggggac gtggtcctcc gcgaccgccg gaagctggcc ggagacggct
     3481 tcgtcgtcgt cgtcgtcacc gccgacgcgc acagcggcga ggtcgtggcc gggcccgacg
     3541 tcatcacccg cggcttcgtc catgacgaga cctctgccga gatgctcgag gaggcgaagg
     3601 cccggaccct ggcctcgctg gccgaggtgg ccgaggagga ggtggccgat ccgagcatcc
     3661 tccgccagca catccggcgc acgctgggcc gctacttctt cgaagcgatt cgccggaaac
     3721 cggtcatcgt tcccgtcatc attgaggtct gatggcgaag aagcggaaga agcggaggac
     3781 gggcggcgcg gtctcggcag ccggccagaa ggtcgcggag cacctcgggc cgcagctgac
     3841 cgacgcgctc ggcatcgggc tgctcgtgct ggccgggctc tcggtgctgg ggatctggct
     3901 ggacgcggcg ggccccctcg ggcgcttcct gacgatcgtg gtcgtcggca tgctcgggct
     3961 ggccgggtac gcattccccg tgctcgccct ctactgggga ctgctgctcg tccggggcac
     4021 ggccgaggag gaccggggcc gcatgttcat cggcctgatc ttcctgctga tgggaggcct
     4081 gggcatcgtc tcgctggtga gggggaatcc gagcccggcg gcaggcttct cggggttcgg
     4141 cgatgccggg ggcctggtcg gcgcgctgac ggcgtggccg ctctcgaagg tgatgtccgt
     4201 ctacggggcg gcgatcgtct gcatcgggct cgtggcgctc ggggctctcg tcttcaccgc
     4261 cacgccgctg cgctcggtgt gggagtggct ggtccggccc aaggacgagg ttctggacct
     4321 caccgaggcc gaggagccgg cgcccaggcg ccgccggcgg aaggagccgg aggtggaggg
     4381 ggagatcctg acgccgaccc tccccgaccc ggagccccag gaggagccga ccccctccgt
     4441 cgtcccgagc cccgcgggtc gcggctacaa gcttcccccg gtcgacatgc tgcggaaggc
     4501 gccggcgtcg ggcgggccgg ggagggacga gaaggagacg atggccatcc tggagcggac
     4561 gctccgcaac ttcggcgtcg cggccagcgt ggtgggcgcg agccggggtc ccacggtgac
     4621 gctcttcgag gtcgaggtct ccgcggggac caaggtgaac aaggtcctca acctcggaga
     4681 cgacatcgcc tatgccctcg ccacgccgga cgtccggatc atcgccccga tccccgggag
     4741 gtccgcgatc ggggtggagg tccccaacaa ggtccgcgac ttcgtgacgc tgggcgacgt
     4801 gctcgggtcg aaggctgcga ggagggagaa gcaccgattg gcggtggccc tggggaagga
     4861 cgtccagggg aaccccctcc tcctcaggct gaacgagatg ccccacctcc tcatcgccgg
     4921 ggccaccggc gccgggaagt cgtcgctgat caacgcgttc gtgacctcga tcctcgttcg
     4981 cgcgcacccc gacgaggtga agctgatgct cgtggacccc aagcgggtcg agctcagcca
     5041 cttcgcggac ctgccccacc tcatctcgcc ggtgatcgtg gccccgaagc gggcggccga
     5101 ggcgctcggt tgggtcgtgc gagagatgga gctccgctac gagtcgctgg ccacggtggg
     5161 gatgcgtgac atcgattcgt acaacggggc gcacgccgac ggctcgctcc gcatcccccc
     5221 ggggcgggac gcggagttcg agcgcctgcc ctacatcctc gtcgtgatcg acgagctgtc
     5281 cgacctcatg atggtggcgc ctcgcgacgt ggaggacgcg atcgtgcgga tctcgcagat
     5341 ggcgcgggcc gtggggatcc acctcgtcgt ggccacccag cggcccagcg tggacgtcgt
     5401 caccgggctg atcaaggcga acatcccgtc ccggatcgcg ttcatgacgg ccagcgcggc
     5461 cgactcccgg gtgatcctgg acgtgggggg cgccgagaag ctcgtcgggc acggcgacat
     5521 gctcttcctg ccctcatcgg cgtccaaggc cgcgcggatc cagggcgcct gggtgaccca
     5581 gcacgagatc gacgcggtcg cagactacgt gcggcagcag cgtgagccgg actatgtcga
     5641 ggggatcgag ggcctgggga agcctcccgc cgacgacgag gacctcgacg cggaggacga
     5701 tctcctccag caggcgatgg agctcgtgat ccactcccag ctcggctcga cctcgatgct
     5761 tcagcggaag ctgaaggtgg ggttcgcccg cgcgggccgc ctcatggaca tgctcgagga
     5821 gcggggagtg gtggggccgt cggtggggtc gaagccccga gacgtgctga tcacctggga
     5881 cgagtacgcg gagtccagga ccggctgaag ggcgggcgtc ggctcagccc gccgcctctg
     5941 ctttgcgctc gagctcgagg ttcaagggcc cgccgagcac cccgcagatc gtcagcacct
     6001 ccagcacgcg aggtgtgggg gcccgcagga cgagccgccc ccgatccgcg tgggaccgtg
     6061 cgagacgcag gatcgctcga agccccgtgg agtcgaggaa ggtcaggtcc gtcaactcga
     6121 ggatgagagg atcgtcgacg agcccgtcca cgctctgctc gagcacgggc acggtggaga
     6181 tgtcgagctc gcctgccagc cggatgcggt ggccggcctc gacgcgcgcc tcgagccgag
     6241 tcgtcatggg tcggtccgat cgagctcgat gcgcaggcgt ccctcccagc ccagggtcgc
     6301 tgcggcgacc aggacccgca gcacgcaggg gcgcgggttg cgcaccgtga tggcgtcgtc
     6361 gcccttcagc tccctcgccg cctcggtcag cacgtggatg cccgcgctgt ccatgaaccg
     6421 cagccccgcg agatcgagga ccgccccgcc ggggctgctc acgacgtccg cgatcgccga
     6481 ggcgaccttc gagcgaacgc tcgtgtcgat ctcgccgatc agcgcgagcg cccgtcgccc
     6541 gaggggctcg atcgagagtc tccttggatc tggggagcgc cgggccccgc accggatggc
     6601 ggcggcgatc gagggagggg accgccccag ccggccggcc gaggatcctg ggcgtatcga
     6661 gcgatggcgg ggggccttca tcgatcgacc cccgtgggat gcgacctccg cggatcggtg
     6721 gctctcgcga tcctggccga cccaccgacc atctcgacga cggcgaagcc gggcgcctcg
     6781 cgcaccacgt agtcgatctc cgggttctcg tgatcgaggg cgatgacgaa cctcgctggg
     6841 taggcgcgca cgcgttcgta cttcacgcga tggatcgtga tcggatcggt gcacgaggtg
     6901 tcgccgcatt cgcagaggta ggcctcgatc ctgttgccga ggtgctcggt gtcgtcgtgg
     6961 gtccactcgt tcatcctccg gaagaccgtc ccgttggaga cctcgacgtc gcgccagctc
     7021 gtcaccatca gccgccctcc tccgaggaga agcgcctcag cagcccgaac ccggccgctc
     7081 cgtcgagcgc ggaccccgtg cccgtgagct cgaggagtcg ccgcacggcc ggtgtgacgc
     7141 cgatcacggc ggtgcgtgcg ccctcgcttc tcatgtgaag gagcgcatcg aacatggctc
     7201 ggagacccgt gggatcggcg aacgtcacgt cgcgagcgtc gaggaggacg ccttccgcgc
     7261 catctgatga gacgctctcc agctcttctg ccaggcgtcg ggcgccggcg aggtccagtg
     7321 cgccgcgcag cagcacacga acgaccccgt tgcgacgctg gatccgaacc ccgagaggct
     7381 cggcggtggg agacgcttcc ggcacgtgac cgcctcgctg tttggtcgac gaagatcgcc
     7441 gaagctgcgg cggccggggg ttggggccct gtcgtgccga gccgttggct ggggaccggc
     7501 accccccacg ctacgactcg gaggcggaac cgtcaagggt gtgccccggg tcagcccgct
     7561 cgggtagggt gaccacatgg agccaccaaa gcaggcgacg tcgctgcgga tcctgatcgc
     7621 ggacggcgac gtggcgcacc tcgaggcgat cgccgagctc gtgaccgccc tgggccacga
     7681 ggtcgtggcc agagagacct cgctggagga gctcggggct cacacggtag ctgagcagcc
     7741 cgatgtcgcg ctcgtcctgg tggaggaggg gcccacggcc ctggatcgga tcgacgagat
     7801 cgtccaggag gccgtctgcc cggtcatcgc ggtgctccgg ggcgaggacc ccgcgttcat
     7861 caacgaggcg gctcgccggg ggatctcggc ctacgtggcc gccgacgatc ccgaggagat
     7921 gcagagctcg atcgacatcg tgcttcggcg cttcggcgag tatcggggac tccaggacgc
     7981 gttcgtccga cgagccgtca cggagcgggc caaggggatc ctgatggagc gccatggggt
     8041 ggatgagcac gcggccttcc tgatgctgcg cgacgaggcg cgcagcacga ggcggaagct
     8101 cgtcgagctg gccgaggcgg tcgtgtccgg ccaccggctg ctcacggcgg atcggcggcc
     8161 ggaccggacg ccctccgggc cggaggagcc cggcgactga cgcggccagc gtttgcgcct
     8221 cccgagggcc gggcatagcc tgccggagag caagcggaag gagggccgca tggacgagct
     8281 cagggacgaa gacatccgca ccgaggatca ggagtccggc ggcgccccgg cgcccgagcg
     8341 cgacgccgat gagcaggacg ccgacggcgt ggaccaggcc gacgggcagg accagggcga
     8401 tgaggtcgac gacgccgacc agggcgacca gggcgatcac gacgacgatg cgaccgacga
     8461 ggacgccgac caggaggacg cctgagccgg cacggagcgc tcggccggct cgtcggtgac
     8521 gaggagcgct tcgcggggga ccactgggga cgccgcccct tcctgaggca gggcgcgggc
     8581 gacttcgcct ctctcctgga tcttcccggc gtggatcggc tcgtcaccgg gggcctgatg
     8641 cggctccccg ggatgcggct cgtccgcgac ggagcgacgg tcccggtcgg cgactatacg
     8701 aggacgatcc ggatcgggtc ccggcgggtt cccgagacgg tccgacccga gcgggtcgtc
     8761 catcacttcg ccaggggggc cacgatcgtc ctgcaggccc tccaccggca gtggccgccc
     8821 atcgccgcgt tctgccgccg gctcgagctc gatctcaccc atccggtcca ggccaacgcc
     8881 tacgtgacgc cggccacctc gcgagggttc gccgtccatc acgacacgca cgacgtcttc
     8941 gtgatccaga cgcacggccg gaagggatgg agggtctacc tgccggtgat cgagctggcg
     9001 gggaaggagc agccctggtc cagtgagctc ggggaccccg gagcgccgat cctggagacg
     9061 gagctctcgc ccggagacgt gctctacatc ccgcgaggct tcccgcacga cgccaccgcc
     9121 cgggaggagg tctcgatcca cgtcacggtg gggatccagg ccaggacctg gctcgacgtc
     9181 tggcggcacg tcatggagca ggcccccgag cacgcgccgt tccgggaggc gctaccgatc
     9241 ggcttcgccc gcgaccggga ggccctggcc ggcgaggtcg cggcccgtct gcccgagctc
     9301 cggtcctggc tggagaaggc cgcgggtccg gaggccgctg cgtccttcgc ggaaggcttc
     9361 tgggacggaa ggcccccggt cctgggcggg cagctcgagc aggtggcaca gctggacgcc
     9421 gtggacgtga cgacgccgct ccgcgtgcgt gcgggcgcga tcttccggcc cggcgtggag
     9481 ggcgaggagg ccgtggcccg gctcgggacc aggatcctcc ggatgcccgg ctgggtggag
     9541 ccggccctcc ggttcgtcgc cgaggcggtg ggcccgttca ccccccggga gcttccgaac
     9601 ctgaatcccg gctcggccct ggtgctctgc cgccggctga tcgtggaggg cgtcctcgag
     9661 gccgatggct gacgcgccga cctgctcggc gctggcgctc gccatggacg agcccctcca
     9721 ggcaaccgcc tcgcgcatcc ggcactggct gctcgtggag cagccggggc gctggggcca
     9781 ccacgccctc ctcgacagcg agctcccgcg cgaggtgggg gagcatctgg cgatcgaggg
     9841 gcaccatcac ggcgtgcggg tgctgctgat caagcggcgc tcgcggggcc ccggcggctc
     9901 caggcggtgc ttcgccgcct tcacggggcg ccgcgagcgc cgcttgatca cgttccagat
     9961 cgacgctccc cgggacctcc tcgacctcga cctcggggcg ctggcccgcg atcgctggag
    10021 cgggctgggc gaggagatcg ccgggccgct cttcgccgtc tgcacgcacg ggaagcacga
    10081 ccagtgctgc gcgcggctgg gcgcgccggt cctcagggcc ctgagccacc gcgaggacac
    10141 atgggaggtc acgcacgtgg ggggcgatcg gttcgcgggc aatctcctgg cgttccccca
    10201 cgggctctac ttcggccggg tgacggggga ggcggccggg agggtggccg acgcctacgc
    10261 gggcggccgg atcgccctcg accacttccg gggccggtcg gccttcaccc ccgccgccca
    10321 ggccgccgag caccacctcc gcgcggcgct gggggtggtc ggggtggacg acgtcgtcct
    10381 gacgggccat cggtcgctgg ggcccctcca ccgcgtggac ttcacggtcc ggggggaggc
    10441 gagggtcgtg gaggtggagg aggtcctcct cgagccgcgg cccctcacct gcaagtcggg
    10501 ggcgccgcgg gcgccgcggt cgttcgtgct ccgggggctc tcctgagtca ggcccctcga
    10561 gcccgctccc gcgcctcggc gatgacccgc agggcggcct cccggtcctc gaacccctcg
    10621 acgcccgagg gcttgtcctc gaggtccttg tacacggcga agaagtgctc gatctcgttc
    10681 cgcagcggct cccggacgtc ctccaggtcc tgcacgtgcg accacatcgg gtcgctcagc
    10741 ggcacgcaga ggatcttctc gtcggcgcct ttctcgtccc acatgcggaa gagccccacg
    10801 ggccgcacgc ggatccggca gccggggaag gtgggctccc cgacgaagac gagcgcgtcg
    10861 agcggatccc cgtccgagcc gagggtcccc tcgatgaagc cgtagtcggc cgggtagacc
    10921 atcgaggtga agagcatccg gtccaggacc agatggccgg tctcgtggtc gacctcgtac
    10981 ttgttcctcg atccccgggg gatctcgacc accacgagca cggtctcgtc gcccacgccg
    11041 cgagtctaga cggggacggc cctcgccgcg gcccgaccct catctgccct acaatctgtg
    11101 cccgtgccga cgcgcgccg
//
LOCUS       DASUTW010000072         4879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_797838, whole genome shotgun sequence.
ACCESSION   DASUTW010000072 DASUTW010000000
VERSION     DASUTW010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797838"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2571)
                     /locus_tag="VF058_02480"
     CDS             complement(<1..2571)
                     /locus_tag="VF058_02480"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX6207203.1"
                     /translation="MSWEEAQPRVLDHAGGGMVVTGPPGSGKSSLLRERFARLVDGGA
                     DPERLVLFVLDRRAARRARDDLIHRLRRSLPDLPVFTVHGFAFRVIGAAFERLGYPEP
                     PQVLSAPEQYAFVRGILQEEDPADWPRLGAYLSEPDFAAQAADFVLRAQERLLDPAEL
                     AGMSSRDEDREMASFYRRYVDAIASAGQVDFASLLFQSAKVLDEEDPGYRHVLVDDYQ
                     DATHAAEGILRSLASRADSVVVAADPGGHVFSYRGGTLEPYRRLDETLGRPERVELPG
                     SRRLSDEGRSLEELERGEASGAQAPPWLSARLYAHPGEEAEAIAHELLRRRVDEDLPW
                     EAMAVVLRRYGGYLTAIRHALARHRIPFVVVAEAAEIAAEPANRPIVDLLRFVFRPER
                     RVELIEPVLTSPIGGLDPHETRRLRREARRAGTPLLHVVLEAAPDDLPGPLRGRVLAL
                     RELVDRMPAVEEERGADGLFHWLWTRLPHVRGLVAEEGPQRDLDALAALAGVLSRFVE
                     RRPGSGIEDYLDTLDAAGFGPDPWIPPEERHPQAVRIISAHRAQGLEFDVVLIAGCLE
                     GEFPSLGARSALVDLESIVDPKPPTERLRDRLAEERALFRLAVSRARLATVLFASTSS
                     GARRPRRPSRFAERLGIVWEPAAEVAPPAVSLRSMEAALRLRLADPEAPRQERLAALA
                     ALPAIGARPSAWWHGRDWTDPGRPIREGEFQTSFSRLEALEACGLLYLGRTELGLDPD
                     ATHYMWLGSVIHEIIERVQKGELPRDVDALVEALDVAWEPEQFPHRAVEHRRRLDAEA
                     MLRRWVEHDRREAIASERAFEFEIAGARVRGRIDAIFQMNNGGLHILDYKSGKNAPTQ
                     K"
     gene            2663..2884
                     /locus_tag="VF058_02485"
     CDS             2663..2884
                     /locus_tag="VF058_02485"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6207204.1"
                     /translation="MDSGRLLRRAQNKELPFSALPAIRELRDYLDALETKIVLLARDK
                     GATWDEIAEAMGITRQALYQRMRNRERRG"
     gene            complement(2895..3275)
                     /locus_tag="VF058_02490"
     CDS             complement(2895..3275)
                     /locus_tag="VF058_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207205.1"
                     /translation="MRAEFVRPEDPEAVVGTAEWDGVRVTLRADDESRATLERVFRVS
                     PVPIDDPSRRALGTSGPVLAEPGDLDWFLAAARVRGRQEGLSVRLVPGTAGGWDPAGA
                     YRRMGDWIGEREASPRETHPASGR"
     gene            complement(3293..4165)
                     /locus_tag="VF058_02495"
     CDS             complement(3293..4165)
                     /locus_tag="VF058_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6207206.1"
                     /translation="MIRIRRVLAPNPGPFTLEGTNTWIVGERPSLVIDPGPDDAGHLL
                     TVADEAEPLEAILLTHRHPDHAPGAARLSAATGAPVYALRPEGDERPIRHNQVLEVGG
                     LAVRAIHTPGHTSDHLVFLLESDGAMATGDTILGRGTSVVDPPDGDMAAYVRSLRELL
                     VLRPQVIYPGHGPVVFDATERIEAYLSHRQQREAQVLEALAEGRSSPEEMVPGIYGGD
                     VPEAMFPHAARSVLAHLLKLEREGRAARTGRLSDNRFVAVPEAPCVRCGRPARVGSSL
                     CRRCSLDALQEGPG"
     gene            complement(4162..4815)
                     /locus_tag="VF058_02500"
     CDS             complement(4162..4815)
                     /locus_tag="VF058_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX6207207.1"
                     /translation="MDPRPAATVVVARPAERGVEVLILRRSASSRFAPGFLVFPGGVV
                     EERDAALAVRLFGRAEEAPRACAVRELYEETSLLLTRGGLEPHPDRPPLEGGAFEPPA
                     LEDVVEIARWVAPESLAERFDAIFYAVAAPRGLDPVPDRVEADDAWWADPEEVVVEST
                     RGESPLMWPTLITLERLASVSSVEEVLSMRIEQLEPGLEERPARVRGAWRRPEGRRP"
BASE COUNT          659 a         1791 c         1827 g          602 t
ORIGIN      
        1 cttctgggtg ggcgcgttct tcccgctctt gtagtcgagg atgtggagcc ctccgttgtt
       61 catctggaag atcgcatcga tgcgcccccg gacccgggcc ccggcgatct cgaactcgaa
      121 ggctcgctcg gaggcgatcg cctcgcgccg gtcgtgctcg acccagcgcc gcagcatggc
      181 ctcggcgtcg aggcggcgcc ggtgctcgac ggcccggtgg gggaactgct cgggctccca
      241 ggccacatcc agcgcctcga ccagggcgtc gacgtcccgg ggaagctcgc ccttctggac
      301 ccgctcgatg atctcgtgga tgaccgagcc caaccacatg tagtgggtgg cgtcggggtc
      361 gaggccgagc tcggtcctcc ccaggtagag gaggccgcag gcctcgaggg cctccagccg
      421 cgagaagctc gtctggaact cgccctcccg gatcgggcgc cccggatcgg tccagtcgcg
      481 gccgtgccac caggcggagg ggcgagcccc gatcgcgggg agagcggcga gggccgcgag
      541 gcgctcctga cggggggcct cgggatcggc gagccggagg cgcagggccg cctccatcga
      601 gcgcagcgag acggcaggag gggcgacctc ggccgccggc tcccagacga tccccagacg
      661 ctccgcgaac cgggaggggc gcctggggcg gcgggctccg ctggaggtcg acgcgaagag
      721 gaccgtggcc aggcgggccc tcgagacggc gagccggaac agggcccgct cctcggccag
      781 gcggtcgcgg agccgctcgg tggggggctt cgggtccacg atcgactcca ggtccacgag
      841 ggccgagcgg gcccccaggc tggggaactc gccctccagg cacccggcga tcagcaccac
      901 gtcgaactcg aggccctgcg cacggtgggc cgagatgatc cggaccgcct gggggtgccg
      961 ctcctcgggc gggatccagg ggtcggggcc gaagccggcg gcgtcgagcg tgtccaggta
     1021 gtcctcgatc cccgagcccg ggcggcgctc gacgaaccgg gagaggaccc cggccagcgc
     1081 cgcgagcgcg tcgaggtcgc gctggggccc ctcttcggcc accaggccgc ggacgtgcgg
     1141 cagccgcgtc cacagccagt ggaagaggcc gtcggctcct cgctcctcct ccacagccgg
     1201 catccgatcg acgagctcgc ggagcgccag cacccgcccg cgcagtgggc cggggaggtc
     1261 gtcgggggcc gcctcgagga cgacgtggag gagcggggtc ccggcgcgcc gggcctctcg
     1321 gcgcaggcgc cgggtctcgt gggggtcgag gccgccgatg ggcgaggtca gcaccggctc
     1381 gatcagctcg acccgccgct cggggcggaa gacgaaccgc aggaggtcca cgatggggcg
     1441 gttggccggc tctgccgcga tctcggccgc ctcggccacg acgacgaagg ggatccggtg
     1501 gcgggccagc gcatggcgga tcgcggtgag gtacccgccg tagcggcgga gcacgacggc
     1561 catcgcctcc caggggaggt cctcgtccac ccgccgccgg agcagctcgt gggcgatcgc
     1621 ctcggcctcc tcgccggggt gcgcgtagag gcgggcgctg agccacggcg gggcctgggc
     1681 cccgctcgct tcccctcgct ccagctcctc gagcgaccgc ccctcgtcgg acagcctccg
     1741 gctcccgggc agctcgacgc gctccggccg gcccagcgtc tcgtccaggc gccggtacgg
     1801 ctccagcgtc cctccccggt aggagaagac gtggccgccg gggtcggccg ccacgaccac
     1861 gctgtcggct cgcgaagcca gcgagcgcag gatcccctcc gcggcgtgcg tggcgtcctg
     1921 gtagtcgtcc acgagcacgt ggcgataccc gggatcctcc tcgtccagca ccttggcgct
     1981 ctggaagagg aggcttgcga agtcgacctg gcccgccgag gcgatcgcgt cgacgtagcg
     2041 gcggtagaag gacgccatct cgcggtcctc gtctcgggag ctcatcccgg cgagctcggc
     2101 cgggtcgagc agacgctcct gggctcggag gacgaagtcg gcggcctggg cggcgaagtc
     2161 gggctcggag aggtacgcgc cgaggcgagg ccagtcggcg gggtcctcct cctggaggat
     2221 cccccggacg aacgcgtact gctcgggcgc ggagaggacc tgggggggct cggggtagcc
     2281 gaggcgctcg aacgcggccc cgatcacgcg gaacgcgaag ccgtggacgg tgaagacggg
     2341 gaggtcgggg agggagcgcc gcagacgatg gatcagatcg tcccgcgccc ggcgggccgc
     2401 gcgtcggtcg aggacgaaca ggacgaggcg ctcggggtcg gcccccccat cgacgagacg
     2461 ggcgaaccgc tcccggagca gggagctctt ccccgatccg ggggggccgg tcacgaccat
     2521 cccacccccg gcatgatcga gcaccctcgg ctgcgcttcc tcccaggaca tcagtcgcat
     2581 gctaccgcca gggtctgaca cgccggccgg gaccgtggcc gcgctgtgcc tccgcccctc
     2641 tcccttcgct agggtttggg acatggactc cggacgcctg cttcggcgag cccagaacaa
     2701 agagctgccc ttctcggcgc tccccgcgat ccgggagctg cgcgactatc tggacgccct
     2761 cgagacgaag atcgtgctcc tcgcccggga caagggtgcc acctgggacg agatcgccga
     2821 ggctatgggc atcacgcgcc aagcgctcta ccagaggatg cggaaccggg agcgccgggg
     2881 gtagcgccgc tgattcaccg cccggacgcg ggatgcgtct cccgcgggct cgcttccctc
     2941 tccccgatcc agtcgcccat ccgccggtac gcgccggccg ggtcccagcc gccggcggtg
     3001 cccggaacga gacggacgct cagcccttcc tgccgtcccc gcacccgggc cgcggccagg
     3061 aaccagtcga ggtccccggg ctcggccagg accgggccgc tcgtccccag ggcccggcgc
     3121 gacgggtcgt cgatcggcac cggcgagact cggaacaccc gctccagcgt cgcccgcgac
     3181 tcgtcgtcgg cccgcagggt gacgcggacc ccgtcccact cggccgtgcc gacgacggcc
     3241 tccggatcct cgggtcggac gaactcggca cgcacgctcg aaggataccc gctcagcccg
     3301 gtccctcctg cagggcgtcg aggctgcacc ttcggcagag cgaggagccc acgcgcgccg
     3361 gccgcccgca ccggacgcac ggggcctccg ggacggccac gaagcggttg tccgagagcc
     3421 tgcccgtccg cgccgcgcgg ccctcgcgct ccagcttgag caggtgcgcc aggaccgacc
     3481 gggcggcgtg cgggaacatc gcctccggga cgtcgccgcc gtagatgccg gggaccatct
     3541 cctcggggga ggacctcccc tcggccagcg cctcgagcac ctgcgcctcc cgctgctggc
     3601 ggtgcgagag gtaggcctcg atccgctcgg tggcgtcgaa gaccaccggg ccgtgtcccg
     3661 ggtagatcac ttggggacgg aggacgagga gctctcggag cgagcggacg taggccgcca
     3721 tgtcgccgtc gggcggatcc acgacgctgg tccctcgtcc caggatcgtg tcccccgtgg
     3781 ccatcgcccc gtcggactcc agcaggaaga cgaggtggtc cgaggtgtgc cccggagtgt
     3841 ggatggcccg aacggcgagt cctcccacct ccaggacctg gttgtggcgg atcggccgct
     3901 cgtccccctc gggtcggagg gcgtagacgg gcgcgccggt ggcggccgag agacgggccg
     3961 ctccgggcgc gtggtccgga tggcgatggg tgaggaggat cgcctccagg ggctcggcct
     4021 cgtcggccac ggtgagcagg tgcccggcgt cgtcggggcc cgggtcgatc acgaggctcg
     4081 gacgctcccc cacgatccac gtgttcgtac cttccagcgt gaaggggccc gggttcgggg
     4141 ccagcacgcg ccggatccgg atcacggacg cctcccctcg ggcctccgcc aggcgccgcg
     4201 caccctcgcg ggccgctcct cgagccccgg ctcgagctgc tcgatccgca tggacagcac
     4261 ctcctccacg ctcgagaccg aggccaggcg ctccagggtg atcagcgtgg gccacatcag
     4321 cgggctctcg ccgcgcgtgc tctccacgac gacctcctcc gggtccgccc accaggcgtc
     4381 gtcggcctcg acccgatcgg ggacggggtc gaggccccgg ggggctgcga ccgcgtagaa
     4441 gatcgcgtca aagcgctcgg cgaggctctc gggggccacc cagcgagcga tctccacgac
     4501 gtcctcgaga gccggaggtt cgaaggcccc gccctccagc ggaggccggt cggggtgggg
     4561 ctcgaggccc cctcgcgtca ggaggaggct cgtctcctcg tacagctccc ggacggcgca
     4621 ggcgcgcggg gcctcctcgg ctcggccgaa gagtcgcacg gcgagcgcgg cgtcgcgctc
     4681 ctcgacgacg ccccccggga acacgaggaa cccaggggcg aaccggctgg acgccgagcg
     4741 ccgaaggatc agcacctcca cgccccgctc ggcggggcgg gccacgacga cggtggcggc
     4801 gggacggggg tccacgcggc gattctggca gaccggacgc ccgggagggc ctgctaggct
     4861 cgccccggtg agcgatctc
//
LOCUS       DASUTW010000073         2759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_409438, whole genome shotgun sequence.
ACCESSION   DASUTW010000073 DASUTW010000000
VERSION     DASUTW010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409438"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VF058_02505"
     CDS             complement(<1..810)
                     /locus_tag="VF058_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207208.1"
                     /translation="MRLRYSRWDGSQDPLGPELSAADILEAISDDLLEGAGPEAALRG
                     LARRGIRGRITGTEALRARLRDARRREQERLDLEGPLREVAEELEEILELERSALSLD
                     SEDDARMRESFIESLPRDPAGRIRELREYRFTDAVAQRKFDELMAKLAEQVLGAYFRQ
                     LAGGMRDLTPEQLARIKDMLAELNAMIEARDRGEPYDFQGFMERYGDMFPGNPRTLDE
                     LLEAMAQRMAAMSRLMASLSPDQQRELRELAEQVMRDMDLAFEADRLGSHLA"
     gene            complement(815..2218)
                     /locus_tag="VF058_02510"
     CDS             complement(815..2218)
                     /locus_tag="VF058_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX6207209.1"
                     /translation="MSRPATIGALREIGYPDRGVKEELRDNLLARLRGGEELFPGVLG
                     FSDSVLPALERGILAGHDLILLGERGQAKTRLIRHLTGLLDEETPIVAGCEVNDHPFR
                     PICARCRALVAEHGDDTPVAWVGRDVRYAEKLATPDTSVADLIGDVDPIKVAEGRHLG
                     DELTIHYGLVPRTNRGIVAINELPDLPERIQVALFNILEERDVQIRGYKVRLPLDLLL
                     VATANPEDYTHRGRIVSPLKDRFGTQVRTHYPDDPEDEITIMRQEARAPASDVPVRVP
                     VFLEEVLAELTRELRDSPHVNQRSGVSVRFSIGNLETLVAAALRRAARTGEAEAVPRP
                     CDLPAVLDASLGRIEFDTIEEGREEEILSRALASAILKVFRRRLAGFDLNPLLDRFEE
                     GLVVSTSDLTPAADMLGQLDRVPGLAKLLSAVGAREESPGVAASALEFALEGLHLSRR
                     LNRDADGRGTYRYESAG"
     gene            2368..>2759
                     /locus_tag="VF058_02515"
     CDS             2368..>2759
                     /locus_tag="VF058_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207210.1"
                     /translation="MPSPATEAPTLAFSSDRGLGFNVFTVPAEGGDPELLVGGEGDDH
                     DPAWSPDGSMVAFARVTDEGRRSSVFLVEADGSGLRQLTSGPHLDRQPAWSPDGMQIA
                     FVRAVPGRGISGIHVLRLGGGRRRLTEPP"
BASE COUNT          347 a          996 c         1008 g          408 t
ORIGIN      
        1 ggccaggtgg ctccccaggc ggtcggcctc gaaggccagg tccatgtccc gcatcacctg
       61 ctcggctagc tcccggagct cccgctgctg gtcgggggag agcgaggcca tgagccggct
      121 catcgcggcc atgcgctggg ccatagcctc cagcagctcg tcgagcgtcc ggggattccc
      181 agggaacatg tccccgtacc gctccatgaa cccctggaag tcgtagggct cgccgcgatc
      241 gcgggcctcg atcatcgcgt tgagctcggc cagcatgtcc ttgatccgcg cgagctgctc
      301 gggggtgagg tcgcgcatcc cgccggccag ctgccggaag tacgcgccga gcacctgctc
      361 ggccagcttc gccatcagct cgtcgaactt ccgctgggcc acggcgtcgg tgaagcggta
      421 ctcgcggagc tcgcggatcc tcccggccgg gtcccgcggc agcgactcga tgaacgactc
      481 ccgcatccgc gcgtcgtcct ccgagtcgag ggagagcgcg ctgcgctcga gctccaggat
      541 ctcctcgagc tcctcggcca cctcgcgaag gggtccctcg aggtcgagac gctcctgctc
      601 ccggcgccgc gcgtcgcgca gccgggccct gagggcctcg gtgccggtga tgcggccccg
      661 gatccctcgg cgagccaggc cccggagggc cgcctcggga cccgcgccct ccaggaggtc
      721 gtcggagatc gcctccagga tgtcggccgc cgacagctcc ggcccgagcg ggtcctggct
      781 cccgtcccag cgcgagtacc ggaggcgcac ggcctcagcc cgccgactcg tagcggtagg
      841 tcccgcggcc gtcggcgtcc cggttcagcc gcctggacag gtggaggccc tcgagggcga
      901 actcgagggc cgacgcggcc acgcccgggc tctcctcccg ggctccgacc gccgagagca
      961 gcttggcgag tccggggact cgatcgagct ggcccagcat gtccgccgcc ggcgtcaggt
     1021 ccgaggtcga gaccacgagc ccctcctcga accggtccag gagcgggttc agatcgaacc
     1081 cggccagccg gcgccggaac accttgagga tcgccgaggc gagcgcgcgg gacaggatct
     1141 cctcctcgcg tccctcctcg atcgtgtcga actcgatcct tcccagcgag gcgtcgagga
     1201 cggccggcag gtcgcacgga cgggggaccg cctcggcctc gccggtccgg gccgcgcgcc
     1261 gcagcgcggc cgccacgagc gtctccaggt tcccgatcga gaatctcacg gagacccccg
     1321 agcgctggtt cacgtggggc gagtcccgga gctcgcgggt gagctcggcc agcacctcct
     1381 ccaggaacac gggcacccgg accgggacgt ccgaggccgg cgcccgggcc tcctgcctca
     1441 tgatcgtgat ctcgtcctcg gggtcgtcgg gatagtgggt gcggacctgg gtcccgaagc
     1501 ggtccttcag cggggagacg atccgccctc ggtgcgtgta gtcctcgggg ttggccgtcg
     1561 cgaccaggag caggtcgagc ggcaggcgga ccttgtaccc gcggatctgg acgtcgcgct
     1621 cctcgaggat gttgaagagg gccacctgga tccgctcggg gaggtccggc agctcattga
     1681 tggcgacgat cccgcggttc gtgcggggga cgagcccgta gtggatcgtg agctcgtcgc
     1741 cgaggtggcg gccctcggcc accttgatcg ggtccacgtc gccgatgagg tcggccacgc
     1801 tcgtgtcggg ggtggcgagc ttctccgcgt agcgcacgtc gcgtcccacc caggccaccg
     1861 gcgtgtcgtc gccgtgctcg gccacgagcg cgcggcatcg ggcgcagatc gggcggaagg
     1921 ggtggtcgtt gacctcgcag cccgcgacga tcggggtctc ctcgtcgagg agcccggtga
     1981 ggtggcggat cagcctggtc ttcgcttgcc cgcgctcccc gaggaggatc aggtcgtggc
     2041 cggcgaggat gcctcgctcc agggcgggga ggaccgagtc gctgaagccg aggacgccgg
     2101 ggaagagctc ctcccctccg cggagccggg ccaggaggtt gtcgcggagc tcctctttga
     2161 cgccgcggtc cgggtacccg atctcgcgga gggctccgat cgttgcgggt cgactcatgg
     2221 accccagcgt accgcgggcg gggaagagag ggttcccggc tggggcgtcc tccgctatcc
     2281 tgtcgcctcg cgtcgaaggg agacgtctcg agtcgccgcc gtgccgccct gctggtcccg
     2341 ttcgtcgccg ccaccctggc cgcccccgtg ccctcgccgg cgaccgaggc cccgacgctc
     2401 gccttcagca gcgatcgggg actcgggttc aacgtcttca ccgtccccgc ggagggcggc
     2461 gaccccgagc tgctggtggg cggggagggc gacgatcacg atcccgcgtg gtcccccgac
     2521 gggagcatgg tggccttcgc ccgggtcacg gatgagggcc gccggtcttc ggtcttcctg
     2581 gtcgaggccg atggaagcgg cctgcgacag ctcacctccg ggccccacct cgatcgtcag
     2641 ccggcctggt cgcccgacgg gatgcagatc gccttcgtcc gggccgtgcc gggccggggg
     2701 atctccggga tccacgtgct gaggctgggg ggcggccggc ggcggctcac cgagccgcc
//
LOCUS       DASUTW010000074         2682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1835720, whole genome shotgun sequence.
ACCESSION   DASUTW010000074 DASUTW010000000
VERSION     DASUTW010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1835720"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VF058_02520"
     CDS             <1..292
                     /locus_tag="VF058_02520"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX6207211.1"
                     /translation="LTGYELSYLVFGSEHSGKGYATEAVRLMTAYLFARQRIERVQLA
                     IHPDNEASRRVAQKAGYTLESLMRRCWFHQGRFHDLEIWSMIRDELPPAPGA"
     gene            350..919
                     /locus_tag="VF058_02525"
     CDS             350..919
                     /locus_tag="VF058_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207212.1"
                     /translation="MDRGRTRPVVSLLVATVVLGGFTLAPVSAHFTQDTRHLGKHAWQ
                     QVIKEKVTALAPRAYGSAAVNGVSFSEPTFVNILRRPVTAPRKGYLHIVAHVMSNDNG
                     SVTGPGLLHYRLTVDGVTVGTEYFMAYGGSPGTIEIGGASAIVKVGKGDHMVALQGKE
                     VGSGSVIDDRDLSVLFVPFGSTTLPVSAP"
     gene            complement(938..2110)
                     /locus_tag="VF058_02530"
     CDS             complement(938..2110)
                     /locus_tag="VF058_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207213.1"
                     /translation="MRRWLAPLVIAATVGCTQTDTVPVIRSADVERPLPEAEAPEPGW
                     YRLPTSPLSPRQNANAFWTGTEVLIVGGRRPFPCFEECAFEPPPFRDGAAFDPAAGEW
                     RRIAKAPIRLGYAQGAVLGETLYLLVPEYYFGWLVSDDEARREPPAFLAYDISTDEWR
                     DLPQPPGRRWPSYLIATEDRLIGYAESHDRGRRPDLVFDPSTEAWTRLPADPLGRAYD
                     RSMVWTGSELVLIGVPYPGEPWIARAAAFDPAADRWRTLPDPGISGLDPVWFWSSGRV
                     VNPSLDLSEEERDGGSRVGAMFDPATSSWEPLPDPPSDYGPYQDLLVGGDEVVVSTSG
                     WALHVPSGTWERLTRPFEGLYANEASVWAGDRLVVWGGHRDGRFVNQGWAWVPPWA"
     gene            complement(2178..2549)
                     /locus_tag="VF058_02535"
     CDS             complement(2178..2549)
                     /locus_tag="VF058_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008737940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6207214.1"
                     /translation="MFGDTEAFSGFAVDDLEKARDFYAETLGLKASVLEEGQLMTLHI
                     AGGRDVFVYAKPDHTPATYTILNFPVDDIDAAVDGLAARGVVFERYEGFEQDENGVAR
                     GQPDIAWFKDPAGNILSVLHR"
BASE COUNT          431 a          936 c          919 g          396 t
ORIGIN      
        1 cctgaccggc tacgagctct cctacctcgt gttcggctcg gagcacagcg gcaagggcta
       61 cgccaccgag gccgtgaggc tcatgaccgc gtacctgttc gcccgtcaga ggatcgagcg
      121 cgtccagctg gccatccatc cagacaacga ggcgtcgcgg cgggtggcgc agaaggccgg
      181 ctacacgctc gagagcctca tgcgtcgctg ctggttccac cagggtcggt tccacgacct
      241 cgagatctgg tcgatgatcc gggacgagct tccaccagcg cccggggcct aaaggctccc
      301 cacagacgtg cgctcatact caaggggtca ttcgaccagg aggtccagca tggatcgcgg
      361 acggacgcgg ccggtcgtca gcctgctcgt ggcgacggtg gtcctggggg ggttcacgct
      421 cgcccccgtc tcggctcact tcacccagga cacgcgacac ctggggaagc acgcatggca
      481 gcaggtgatc aaggagaagg tcacggccct cgctccgcgc gcgtacggaa gcgccgcggt
      541 caacggggtg tccttctcgg agccgacctt cgtgaacatc cttcgacggc cggtcacggc
      601 tccccgtaag gggtacctcc acatcgtggc ccacgtgatg tccaacgaca acggttcggt
      661 gacgggcccc ggtctgctcc actaccgatt gaccgtcgac ggcgtgaccg tagggaccga
      721 gtacttcatg gcgtacggcg gatcgccggg gaccatcgag atcggtgggg cgagcgcgat
      781 cgtgaaggtg gggaagggcg accacatggt ggcgctccag gggaaggagg tcgggtcggg
      841 cagcgtcatc gacgaccgcg atctcagcgt gctcttcgtg cccttcggct ccaccaccct
      901 cccggtctcc gcgccgtagg cggccggcag agctcggtca ggcccacggg ggaacccagg
      961 cccacccttg gttcacgaat cgtccgtcgc ggtgcccgcc ccatacgacg aggcggtcgc
     1021 ccgcccagac ggaggcctcg ttcgcgtaga gcccctcgaa gggacgcgtg aggcgctccc
     1081 acgtgccgga gggcacgtgg agggcccatc ccgaggtgga caccacgacc tcgtcacccc
     1141 ccacgagcag atcctggtag ggcccgtagt ccgagggcgg gtccggcagc ggctcccaag
     1201 aagacgtcgc agggtcgaac atcgccccga cacggctgcc gccgtcgcgc tcctcctcgg
     1261 agagatcgag cgaggggttg accacgcgac cggacgacca gaaccagacc gggtccaggc
     1321 cgctgatccc cggatcgggc aacgtcctcc agcgatccgc ggccggatcg aatgccgccg
     1381 ctcgcgcgat ccaaggctcc ccgggatagg gcacgccgat caggaccagc tcgctccccg
     1441 tccacaccat gctgcggtcg taggcacggc cgagcggatc cgcaggaagc ctggtccacg
     1501 cctcggtcga gggatcgaag acgagatcgg gacgccgccc acggtcgtgt gactccgcat
     1561 acccgatgag gcggtcctcg gtggcgatga ggtagctggg ccagcgccgt cccgggggct
     1621 gagggaggtc ccgccactcg tccgtggaga tgtcgtaggc gaggaaggcc ggcggctccc
     1681 tccgcgcctc gtcgtcgctc accagccagc cgaagtagta ctcgggcacc aggaggtaca
     1741 gggtctcccc gaggactgct ccctgcgcgt acccgagccg gatgggagcc ttcgcgatcc
     1801 tcctccactc gcccgcggcc gggtcgaagg cggcgccgtc gcggaacggc ggcggctcga
     1861 acgcgcactc ctcgaagcac gggaaggggc ggcggccgcc cacgatcagc acctcggtgc
     1921 cggtccagaa cgcgttggcg ttctggcggg ggctgagggg cgatgtgggg agccggtacc
     1981 agcccggctc gggggcctcg gcctccggga gggggcgctc cacgtccgcc gacctgatca
     2041 cggggacggt gtccgtctgc gtgcatccca ccgtcgccgc gatcacgagc ggggcgagcc
     2101 accttcgcat cgtggcctca tgatgaaccc tttcgactgg aagcgcgcga ggaaccgacc
     2161 cggccgggaa gggccgatta cctgtggagc acggagagga tgttgcccgc cgggtccttg
     2221 aaccacgcga tgtcgggctg gccgcgcgcg acgccattct cgtcctgctc gaagccttcg
     2281 tagcgctcga agacgacccc gcgcgcggcc agcccgtcca ccgcggcgtc gatgtcgtcg
     2341 acggggaagt tgaggatcgt gtaggtggcc ggcgtgtggt cgggcttcgc gtacacgaag
     2401 acgtcccgac cgcccgcgat gtggagcgtc atcagctgac cctcctccag aaccgacgct
     2461 ttcaggccga gcgtctcggc atagaagtcc ctggccttct ccagatcgtc cacggcgaag
     2521 ccgctgaacg cctcggtgtc cccgaacatg ctgagctcct ctcgatcgcc ttcgtccata
     2581 gtgacgatcc ggagcccggc tcctcatcgc cggtcggggc gggcggattc gaaccgccga
     2641 cctcgtgctc ccaaagcacg cgcgctgcca agctgcgcca cg
//
LOCUS       DASUTW010000075         6257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_540159, whole genome shotgun sequence.
ACCESSION   DASUTW010000075 DASUTW010000000
VERSION     DASUTW010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540159"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VF058_02540"
     CDS             <1..670
                     /locus_tag="VF058_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX6207215.1"
                     /translation="ATLTAQQPENNIVRTALEALAAVFGGTQSLHTNSFDEALALPSE
                     RAARIALRTQQVVAHEAGVIDTVDPLGGSYFVESLTSEIETRAWEYLEKIDGMGGSVQ
                     AIEAGWIQDEIHDAAYRIQQGVESGERVVVGVNRFVEDEEDPVELERLDEEAVARQVQ
                     RLRDLRERRDGAAVERALDAVEAAARETDNLLYPMREALKARATLGEVSDRLRAVFGE
                     HGPR"
     gene            667..1362
                     /gene="npdG"
                     /locus_tag="VF058_02545"
     CDS             667..1362
                     /gene="npdG"
                     /locus_tag="VF058_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEX6207216.1"
                     /translation="MSDRVAIVGGTGDLGFGIALRLGRVGQPVVIGSRSAEKAADAVA
                     RAKEVIGPDAPVEGAENPTAVADAPIAFVTVPYAGQAATYKGLREHWARGAIVCDTTT
                     PLATAVGAAATQVLRPWQGSAAEQAASYLPDHVAFVSGFHTVSAEDLEDLDDDPSSDV
                     LLCGGDAEPKAAIGGLVERMAMRWVDCGPLTMARILEPITAVLIRVNRMYGIRHAGVR
                     MTGRDDWGAADRA"
     gene            complement(1400..2659)
                     /locus_tag="VF058_02550"
     CDS             complement(1400..2659)
                     /locus_tag="VF058_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX6207217.1"
                     /translation="MGSGLYAALTEANLLVPHEETDEPGVSDQAYRVIEPRRVPFISY
                     PYEWCFGQLKDAAMATLRIQRIALRHGMSLRDASAYNVQFLDGRPVLIDTLSFERLPE
                     NRPWIAYRQFCQHFLAPLALMAFRDVRLAQLLRVHIDGIPLDLTARLLPRRARLRPAL
                     FLHIVAHARSQRRHAGEVVTAERGRRFTPRAFVGLIDSLWSAVRRLRWRPGRSVWSQY
                     YEESGSYSPEAFEHKREIVQKLVAETEPADVWDLGGNVGTFARIASERGVPTVCFDAD
                     IAAVELNYRREVEARERNLLPLVLDLTNPSPPIGWENRERRSLAERGPADLVLALALI
                     HHLAIGNNVPLPRLATFLEGLCRRLVVEFVPKEDPKVQILLATREDVFPGYTREGFEQ
                     AFGKRFEIEARIGIRDSSRIIYLMRRR"
     gene            complement(2772..2957)
                     /locus_tag="VF058_02555"
     CDS             complement(2772..2957)
                     /locus_tag="VF058_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016812228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207218.1"
                     /translation="MTPAAQAYLDPGSGSFIFQVIIGGLLGAAVAVKAFWQRIWGFLA
                     QRKADREVEDKVSEEIG"
     gene            3121..5268
                     /locus_tag="VF058_02560"
     CDS             3121..5268
                     /locus_tag="VF058_02560"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX6207219.1"
                     /translation="MTDPSAPSDRGSAAPTVLRQELDYLLQVLGLNAFAVAQPVLDVM
                     GKSPETFIFRGADARQIILFSIAVTVIPVLALSALAASTRVFGRQARRFAHLVVLGLL
                     ASVIAMQVLKRATPLRDLALLLPALAIGGVAAWLVSRARAAQTYIRLAAIAPIPFIAI
                     FLFASPVSRLLETAETGGVAEDARAVPVVMVMFDEFPLGSLLDRRGRINETLYPNIAE
                     LAATSTWYRNYTVTERRTGQSIATKLTGNMLEDRSKRPLAADHPDNLFTLLSESHRLA
                     VFEPVTAFCPEDLCQGSHEESEQSLDVRSSRGGVPALLSDAALVWSELSLPTESTRDV
                     TTQFEELAEEAAAEEGAAPAGEADQGASLEVAFVESLRPGRPALHYVHLMLPHAPWRR
                     FPDGDTYQILEGREDLPLGRGDTNFRPWVEEGWAVQLARQRHILQVQYTDRFIGRVME
                     RLRRVGMFDETLLIITADHGVSLQPGRERRVPTPDNMHEIYWVPLFIRAPGQQEGIED
                     DRNLMAPDLLPTIADLLGTEVPWETDGRSAMDPVYDRGPVKEFVRREDEETPLPEVTM
                     TIRSREAQRRLRREAYAPEAGCSGTVDLCPYLVGPGASLIGQSVDTFTLGPPSGLSAD
                     LVLPHTLRVDGEGSLPGLVLGTLTGISEGYEGQILVAVNGSVAGISETWTEDGMPGWF
                     GVLAPERLLRPTGNELWLFELEGSVLRPIPIAG"
     gene            complement(5349..>6257)
                     /locus_tag="VF058_02565"
     CDS             complement(5349..>6257)
                     /locus_tag="VF058_02565"
                     /inference="COORDINATES: protein motif:HMM:NF014804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX6207220.1"
                     /translation="GTVEFLVEDDGSFYFLEMNTRLQVEHPVTEMVTGLDLVRMQIEV
                     ALGEKVDPGTPDLRGHAIECRINAEDPAADFLPGPGLVRRLVLPSGPFVRVDAGVTEG
                     RTIPGDYDSLFAKLIVWGEDREAARRRMLRALDELQVEGVPTTVPFHRWVLNTIAFRE
                     GSHTTRWVERALEEGMFRPEEAEPPSPAPPTAPGLLVVEVGGRRVPVRVWGDDRAAPP
                     PAPESAARAHHAGAHGAVVAPMQGTILKVLVEEGQEIEAGDVVCILEAMKMENHIAAT
                     QDGTISSVAVSVGDVVQSGQAIATIG"
BASE COUNT          960 a         2173 c         2182 g          942 t
ORIGIN      
        1 ggccacgctc acggcccagc agcccgagaa caacatcgtg cggacggccc tcgaggcgct
       61 ggccgccgtg ttcggcggca cgcagtcgct gcacacgaac tcgttcgacg aggcgctggc
      121 gctgccgtcg gagcgcgcgg ctaggatcgc cctccgcacc cagcaggtgg tcgcccacga
      181 ggccggggtg atcgacaccg tggacccgct gggtggctcc tacttcgtgg agtcgctgac
      241 gagcgagatc gagacgcggg cctgggagta cctggagaag atcgacggca tgggcggctc
      301 ggtgcaggcg atcgaggcgg ggtggatcca ggacgagatc cacgacgccg cctaccggat
      361 ccaacagggg gtggagtcgg gggagcgtgt agtggtcggg gtgaaccggt tcgtcgagga
      421 cgaggaggac ccggtggagc tggagcgcct cgacgaggag gcggtggcgc ggcaggtgca
      481 gcgcctccgt gatctgcggg agcgtcgtga cggcgcggcg gtggagcggg ccctcgacgc
      541 agtggaggcg gcggcgcggg agaccgacaa cctcctctac ccgatgcggg aggccctgaa
      601 ggcccgggcc acgctcggcg aggtctccga ccgtctccgg gccgtcttcg gggagcacgg
      661 ccctcggtga gcgaccgcgt cgcgatcgtg gggggcacgg gcgacctcgg cttcggcatc
      721 gccctccgct tggggagggt gggccagccc gtcgtgatcg gatcccggtc ggccgagaag
      781 gccgccgacg cggtggcccg ggccaaggag gtcatcggcc ccgacgcccc ggtggaggga
      841 gcggagaacc cgacggcggt ggccgacgcc ccgatcgcgt tcgtaacggt gccgtacgcg
      901 gggcaggcgg ccacgtacaa ggggctgagg gagcactggg cccggggggc gatcgtctgc
      961 gacacgacca cgccgttggc caccgcggtt ggcgccgccg caacgcaggt actgcggccg
     1021 tggcaggggt cggccgccga gcaggcggcc tcgtaccttc ccgaccacgt ggcgttcgtc
     1081 tccgggttcc ataccgtcag cgccgaggat ctcgaggacc tggatgacga cccctcctcg
     1141 gacgtcctgc tctgcggcgg cgatgcggag cccaaggccg cgatcggcgg gctcgtggag
     1201 cgcatggcga tgcggtgggt cgactgcggg ccgctgacga tggccaggat cctggagccg
     1261 atcacggccg tgctgatccg cgtgaacagg atgtacggca tccgccacgc cggcgtccgc
     1321 atgaccggcc gcgacgactg gggggccgcc gaccgggcct gaggccgatc tctggcgcgc
     1381 gtccttgcgt cgctacccct caccgacgcc gcatcaggta gatgatgcgg gacgagtccc
     1441 ggatcccgat acgcgcctcg atctcgaagc gcttcccgaa cgcctgctcg aacccctcgc
     1501 gcgtgtagcc ggggaacaca tcttccctcg tggccagaag gatctgcacc ttcggatcct
     1561 ccttcggaac gaactcgacg accaacctgc gacagaggcc ctccaggaac gtcgccaacc
     1621 gcggcagcgg gacgttgttg ccgatggcga ggtggtggat gagcgctagg gccagcacga
     1681 ggtccgcggg acctcgctcc gccagggaac ggcgctcccg attctcccat ccgatcggcg
     1741 ggctggggtt cgtgagatcg aggaccaggg gtagcaggtt gcgctcgcgc gcctccacct
     1801 cgcggcggta gttcagctcc accgccgcga tgtcggcgtc gaagcacacc gtcggtaccc
     1861 cacgctcgga cgcgatgcgg gcgaaggtcc ccacgttccc cccgaggtcc cagacgtccg
     1921 ccggctcggt ctccgccacg agcttctgca cgatctccct cttgtgctcg aaggcctcgg
     1981 gggagtagct ccccgactcc tcgtagtact gggaccagac ggagcggccg ggccgccatc
     2041 ggaggcgacg aacggcggac cagaggctgt caatgaggcc cacgaatgcc cggggcgtga
     2101 agcggcgacc tcgctccgcg gtcacgacct cgcccgcgtg gcggcgctgg ctccgtgcgt
     2161 gggcgacgat gtgcaggaag agggccggtc gcagcctggc tcgacgcgga agcagccgcg
     2221 cggtcagatc cagggggatc ccatcgatgt ggactcggag cagctgggcc agccggacgt
     2281 cgcggaacgc catcagggcg agcggggcca ggaagtgttg gcagaactgt cggtaagcga
     2341 tccacggtcg gttctcgggg agccgctcga acgagagcgt atcgatgagc accggacgcc
     2401 cgtccaggaa ctgcacgttg taggcgctcg cgtccctgag gctcatcccg tgccgcaggg
     2461 cgatccgctg gatccgaagc gttgccatcg cggcgtcctt gagctgcccg aagcaccact
     2521 cgtacgggta ggagatgaag ggcaccctgc ggggctcgat gacccggtag gcctggtccg
     2581 atactccggg ctcgtcggtc tcctcgtggg ggacgagcaa gttcgcctct gtcagagccg
     2641 cgtacagccc agatcccatc aggctgtcgt aatgctctcg gtggactcga ttgacctgcc
     2701 ggaagaggcg cccccctctc tcgaagagga cgccgctcgg gtcccggaac gattccggga
     2761 cgacctcgtc gtcagccaat ctcctcggaa accttgtctt cgacctcccg atccgccttc
     2821 cgctgggcca ggaagcccca tatccgctgc cagaacgcct tcacggctac tgccgcgccg
     2881 agcagccccc cgatgatcac ctggaagatg aagctgcccg agcccggatc caggtaggcc
     2941 tgggcggcag gcgtcacgat caggacgaag tacgctgcga agatcgcaac ccacaggatc
     3001 cttcggctca tcgaacctcc tccacctgtc ggatcgagcg cgtcgagccc caagccgacg
     3061 ccggggatag tcttgtccac ctcgggcccg ttgtctaccc tcagctataa tatcggatcg
     3121 atgacggacc ccagcgctcc ctcggaccgg gggtccgctg cacccaccgt cctgcggcag
     3181 gagctcgact accttctcca ggtcctggga ctcaacgcgt tcgcggtggc gcagccggtc
     3241 ctcgacgtga tgggcaagag ccccgagacg ttcatcttcc ggggcgcgga cgcgcggcag
     3301 atcatcctgt tctcgatcgc ggtgactgtg atcccagtcc tcgctctctc cgcgctcgcg
     3361 gccagcacca gggtgttcgg gcgccaggcc cggcggttcg cccacctcgt cgtcctcggc
     3421 ctcctcgcat cggtgatcgc gatgcaggtc ctgaagaggg ccacaccact gcgggatctc
     3481 gcactcctcc tcccggcgct ggcgatcggc ggggtcgcgg catggctggt gtcgagggcc
     3541 cgtgcggccc agacctacat ccggctggcc gcgatcgcgc cgatcccctt catcgccatc
     3601 ttcctcttcg cctcacccgt gtcccggctc ctggagacgg cggagacagg gggcgtcgcc
     3661 gaggatgctc gggctgtccc cgtcgtgatg gtcatgttcg acgagttccc tctcggctcc
     3721 ctcctcgatc ggaggggacg gatcaacgaa accctctatc cgaacatcgc cgagttggcc
     3781 gcgacctcga cctggtaccg gaactacacg gtgaccgagc gacgcacggg gcagtccatc
     3841 gcgacgaagc tcaccggcaa catgctcgag gatcgctcga agcgacccct cgccgccgat
     3901 cacccggaca atctcttcac gctcctctct gagagccacc gcctcgccgt cttcgagccc
     3961 gtcacggcgt tctgtcccga ggacctctgt caaggctccc acgaggagag cgagcagagc
     4021 ctcgacgtcc ggtcgtcgcg tggaggggtg cctgctctgc tgtccgacgc cgcgctggtc
     4081 tggagcgagc tctcccttcc cacggagagc accagggatg tgaccacgca gttcgaggag
     4141 ctggccgaag aggcggcagc ggaggagggg gcggcgcccg cgggcgaggc ggatcagggg
     4201 gcctccctgg aggtcgcgtt cgtggaatcc ctccgtccgg gcagacccgc gctccactac
     4261 gtccatctga tgctcccgca cgcgccctgg aggcggtttc cggacgggga cacctatcaa
     4321 atcctggagg ggcgggagga cctccccctc ggccggggtg acacgaactt ccggccgtgg
     4381 gtcgaagagg gctgggcggt ccagctcgcc cgccagcggc acatcctcca ggtccagtac
     4441 acggatcgct tcatcggccg ggtgatggag cggctccggc gggtcggcat gttcgacgag
     4501 actttgctca tcatcacggc ggaccacggc gtcagcctcc agccgggacg ggagcgacgc
     4561 gtcccgaccc ccgacaacat gcacgagatc tactgggtcc ccctgttcat tagggcaccc
     4621 ggccagcagg aaggcatcga ggacgaccgg aacctgatgg cgccggacct gctcccaacc
     4681 atcgcggacc tgctgggaac cgaggtgccg tgggagacgg acggtcggtc ggcgatggat
     4741 cccgtatacg accgggggcc ggtcaaggag ttcgttcgcc gggaggacga agagacccct
     4801 ctacctgagg tcaccatgac gatcagatcg agagaggccc agcgacgact ccggcgggag
     4861 gcgtacgcgc cggaggccgg ctgctcgggg acggttgacc tctgccccta cctcgtcggc
     4921 ccaggagcct ccctgatcgg ccagtcggtg gacaccttca ccctcgggcc tccctccggg
     4981 ctctccgccg acctcgtgct gccacacacc ctccgtgtcg acggagaggg atccctcccc
     5041 ggcctggtcc tcggcaccct gacgggtatc tcagaaggct acgaaggcca gatcctcgtt
     5101 gccgtgaatg ggtccgtcgc gggaatatcg gagacatgga ccgaggacgg tatgcctggg
     5161 tggttcggcg tcctggcgcc ggaacgcctg ctcaggccca ccggaaacga gctgtggctc
     5221 ttcgagctcg agggctccgt ccttcggccg atccccatcg ccggatagcg atcgtcggcc
     5281 aacccgtccc cgggggcgta gggttgcgcg tgctcccgaa cggaggacgc cgctcgagga
     5341 gcctccggtc agccgatcgt ggcgatcgcc tgcccgctct gcacgacgtc gccgacggag
     5401 acggccaccg agctgatcgt cccgtcctgg gttgccgcga tgtggttctc catcttcatc
     5461 gcctcgagga tgcagacgac gtcgcccgcc tcgatctcct gaccctcctc cacgaggacc
     5521 ttcaggatcg tgccctgcat cggggcgacg accgcgccgt gggcgccggc gtggtgcgcg
     5581 cgggcggcgg actcgggcgc gggcgggggg gcggcgcggt cgtcacccca cacccggacc
     5641 ggcacccgac gccccccgac ctcgaccacg aggaggccgg gagcggtggg gggcgcgggc
     5701 gaggggggct cggcctcctc cggccggaac atcccctcct ccagggcccg ctccacccac
     5761 ctcgtggtgt ggctcccctc gcggaacgcg atcgtgttca gcacccaccg gtggaagggg
     5821 accgtggtgg ggacgccctc cacctggagc tcgtccagcg ctcggagcat gcggcggcga
     5881 gcggcctcgc gatcctcgcc ccagacgatg agcttggcga agagcgagtc gtagtcgccg
     5941 gggatcgtcc gcccctcggt gacccccgcg tccacgcgga cgaaaggccc cgacgggagg
     6001 acgagccggc gcaccaagcc cgggccgggg aggaagtcgg cggcggggtc ctcggcgttg
     6061 atccggcatt cgatcgcgtg gccccgcagg tcgggggtgc ccgggtccac cttctcgccc
     6121 agggccacct cgatctgcat ccgcacgagg tcgaggccgg tcaccatctc ggtcaccggg
     6181 tgctcgacct ggagccgggt gttcatctcc aggaagtaga aggagccgtc gtcctcgacc
     6241 aggaactcga ccgtgcc
//
LOCUS       DASUTW010000076         3114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_281481, whole genome shotgun sequence.
ACCESSION   DASUTW010000076 DASUTW010000000
VERSION     DASUTW010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281481"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1315
                     /locus_tag="VF058_02570"
     CDS             <1..1315
                     /locus_tag="VF058_02570"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX6207221.1"
                     /translation="AVMAGVGGSSEDVAVAAQDSAFRIEGEGTGEIVEVELDAIEVEQ
                     EIADGVTYHAWTFGGTAPGPVIRAKVGDTIRFTLNNKTNLQLSHSIDFHAAQTPWSVN
                     YQPVLPGDSLTFDWVARFPGVFMYHCGVPPVLHHIANGMYGAIIVTPADPLPELEPAR
                     EYVLVSSEFYPGDDPVQGVYEGDAAKMEAAEPEYVVFDGKANRYLDEPLVARPDELIR
                     LWVMNAGPTIDNAFHVIGALIDHVYPDGNPTNALNGMQTWNIPPGGGAMFELRIPDEG
                     QYPFVNHAFAYTGLGALGILDISEDAPPAPESYPVYGNPWDGGVTETVGTVDGEAPAQ
                     PEEDGEAAGGGGGGDGLVLTMADLAYEQTSLMVQPGDTITLVNEDPMVHDVTVDELDL
                     QATAGPQETVEFTIPDDASGTFTFYCSIPGHREAGMEGSMMVGAH"
     gene            1380..1934
                     /locus_tag="VF058_02575"
     CDS             1380..1934
                     /locus_tag="VF058_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX6207222.1"
                     /translation="MLALWIALGVVGVLLLAFVLVYNRLVRNRNRVDNAWAGIDVQLR
                     RRYDLIPNLVETVKGYASHERKLFEEVTRARAHGIEAEGIDQQAAAENEITRGLRQLF
                     AVAENYPQLRANENFLALQEELTATESKIAFARQFYNDQVLRYNTLIQQFPSLIVART
                     TNHTTRDFFEIEEGSRAPVEVDLS"
     gene            1941..2846
                     /locus_tag="VF058_02580"
     CDS             1941..2846
                     /locus_tag="VF058_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX6207223.1"
                     /translation="MYEQIAKNRRRSVLLVVLVLALAVALGYVLGLLFQYGIAGAIVA
                     LGIAIVMTWSSYFYGDRVVLRVSGARPAPREEYPQYHNVVEGLAIAAGIPKPDLYVVP
                     EEAPNAFATGRNPERASIGVTEGLLETLNRVELEGVMAHELAHVRNRDILFGTLVATL
                     VGVVVLLAIFARRSIFWGGMRGGRRSGRSGGPAMLVIIVVGLVLAILAPLGAQMIRLA
                     VSRRREYLADADGALLSRYPPGLASALRKIAQNTKPMRVANSATAHLWLSEPSVTPGH
                     GLTRWERLFATHPPIEDRIRILEEM"
BASE COUNT          502 a         1064 c         1095 g          453 t
ORIGIN      
        1 cgcggtgatg gcgggcgtgg gcggctcgtc cgaggacgtc gccgtcgccg cgcaggactc
       61 ggcgttccgg atcgaggggg aggggaccgg tgagatcgtc gaggtcgagc tcgacgcgat
      121 cgaggtcgag caggagatcg ccgacggggt cacctaccac gcctggacct tcggagggac
      181 cgctccgggg ccggtgatca gggcgaaggt cggcgacacc atccgcttca cgctcaacaa
      241 caagacgaac ctccagctgt cgcactcgat cgacttccac gcggcccaga ccccgtggag
      301 cgtcaactac cagccggtcc tgccgggaga cagcctcacc ttcgactggg tggcccggtt
      361 ccccggcgta ttcatgtacc actgcggggt cccgccggtg ctgcaccaca tcgccaacgg
      421 gatgtacgga gcgatcatcg tgacgccggc cgaccccctg cccgagctcg agcccgcgcg
      481 cgaatacgtc ctggtctcga gcgagttcta cccgggcgac gatcccgtgc agggcgtata
      541 tgagggcgat gcggccaaga tggaggccgc cgagcccgag tacgtcgtgt tcgacggcaa
      601 ggcgaaccgc tacctcgacg agccgctggt cgctcgcccc gacgagctga tccggctctg
      661 ggtgatgaac gccggcccca cgatcgacaa cgccttccac gtgatcggag cgctgatcga
      721 ccacgtctac ccggacggca accccacgaa cgcgctgaac ggaatgcaga cgtggaacat
      781 ccctccgggc ggcggcgcca tgttcgagct tcggatcccc gacgagggcc agtacccgtt
      841 cgttaaccac gcgttcgcct acacggggct cggggcgctc ggcatcctcg acatcagcga
      901 ggacgcgccg cccgcgccgg agtcctaccc cgtgtacggc aacccgtggg acggcggcgt
      961 cacggagacg gtcggcacgg tggacggcga ggcgcccgcc cagcccgagg aggacgggga
     1021 ggccgccggc ggcggcggag gcggcgacgg cctggtgctg acgatggccg acctcgccta
     1081 cgagcagacg agcctcatgg tccagccggg cgacacgatc actctggtga acgaggaccc
     1141 gatggtgcac gacgtcacgg tggacgagct cgacctccag gccaccgcgg ggccacagga
     1201 gaccgtcgag ttcacgatcc cggacgacgc ctccggcacc ttcaccttct actgctcgat
     1261 cccgggccac cgggaggcgg gcatggaggg cagcatgatg gtcggggccc actgaccgga
     1321 gcggggaggg gggtcggcgt gcaggcgtcg acccccccgc tcgctggtac gatacgaacg
     1381 tgcttgccct ctggatcgcg ctgggcgtcg tcggggtgct cctcctggcc ttcgtcctcg
     1441 tctacaacag gctcgtccgc aacaggaacc gcgtggacaa tgcctgggcc ggcatcgacg
     1501 tccagctccg ccgtcgctac gacctcatcc cgaacctcgt ggagacggtc aagggatacg
     1561 cttcgcacga gcggaagctg ttcgaggagg tcaccagggc ccgggcccac gggatcgagg
     1621 ccgaggggat cgatcagcag gcggcggccg agaacgagat cacgcgaggg ctccgccagc
     1681 tcttcgccgt ggccgagaac tacccccagc tccgggcgaa cgagaacttc ctcgcgctgc
     1741 aggaggagct gaccgcgacg gagtcgaaga tcgccttcgc ccggcagttc tacaatgacc
     1801 aggtcctgcg ctacaacacg ctgatccagc agttcccatc actcatcgtg gcgcggacga
     1861 cgaaccacac gacgcgcgac ttcttcgaga tcgaggaggg atcccgggcg cccgtcgagg
     1921 tggacctctc ctaggcgtcc gtgtacgagc agatcgccaa gaacaggcgc cggtcggtcc
     1981 tgctcgtcgt gctcgtcttg gcgctggcgg tggcgctggg gtacgtgctg ggcctgctgt
     2041 tccagtacgg gatcgccggc gcgatcgtcg cgctcgggat cgcgatcgtc atgacgtggt
     2101 cctcgtactt ctacggagat cgcgtcgtgc tgcgcgtcag cggagcgcgg cccgccccgc
     2161 gcgaggagta cccccagtac cacaacgtcg tggaggggct ggcgatcgcg gctgggatcc
     2221 ccaagcccga tctctacgtg gtgccggagg aggccccgaa cgcgttcgcc acgggaagga
     2281 accccgagcg cgcctcgatc ggggtcaccg aggggctcct cgagaccctg aaccgcgtgg
     2341 agctcgaggg cgtgatggcc cacgagctcg cccacgtgcg gaaccgagac atcctcttcg
     2401 ggacgctcgt ggcgaccctg gtgggcgtcg tcgtcctgct cgcgatcttc gcccgacgct
     2461 cgatcttctg gggagggatg cggggcggtc gccgctcggg gcggagcggg ggcccggcga
     2521 tgctcgtcat catcgtggtg gggctggtgc tggcgatcct ggcccccctc ggcgcccaga
     2581 tgatccgcct cgcggtctct cgccggcggg agtacctggc cgacgccgac ggggcgctcc
     2641 tcagccggta cccgccgggc ctggcctccg cgctccggaa gatcgcccag aacacgaagc
     2701 cgatgcgcgt cgccaacagc gccacggcgc atctctggct gtcggagccg tccgtgacgc
     2761 ccgggcacgg gctcacgagg tgggagcggc tcttcgccac gcatccgccc atcgaggacc
     2821 gcatccgcat cctcgaggag atgtgatcgg atctccccca cctcccctgg ggcggcggtc
     2881 aaactgagac gagaggagta gcatctcccg ccgtgcccgc agccggagag atcgaccagg
     2941 ggaagatcga ggaggcttca cgttgagcac cagagggaag gccgtgctgg cgagcgtctc
     3001 ggccgtgatc gtcgtcgcgg cggccgggat cgggtacctg atgttcacgg ggcagagcga
     3061 cgccatcccg ttcctggggg gaacgcggga gccggagcgc tgcccgctga cggg
//
LOCUS       DASUTW010000077         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1188841, whole genome shotgun sequence.
ACCESSION   DASUTW010000077 DASUTW010000000
VERSION     DASUTW010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188841"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="VF058_02585"
     CDS             complement(<1..140)
                     /locus_tag="VF058_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HEX6207224.1"
                     /translation="MAEALGMIECRSFPAMVEAADAMVKAAKVELVSFEETGGGYVTA
                     VVR"
     gene            complement(152..784)
                     /locus_tag="VF058_02590"
     CDS             complement(152..784)
                     /locus_tag="VF058_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017720546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207225.1"
                     /translation="MAKEIELRAYCFLDSLQPQYAAFLGTVAQGFLPLAGDASVYVEV
                     APGIEINRLTDVALKSTTVKPGMQIVERYFGMLEVHSPEQAEARAAGAAILDAIELKE
                     EDRIKPRVLSSQIIRRIDDHQAQLINRVRHGEMLIPGQTLYVLECEPAAYAALAANEA
                     EKAAEINILEVRSFGSMGRIYLGGEERDIDVGWQAAVAALEGLEGREATR"
     gene            complement(800..1651)
                     /gene="deoC"
                     /locus_tag="VF058_02595"
     CDS             complement(800..1651)
                     /gene="deoC"
                     /locus_tag="VF058_02595"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX6207226.1"
                     /translation="MSVREDLIEAVTSEVLAALAPEERCPDCRGSCAAHVPDKVRAIV
                     DNGAHRISYTGNGSSVPSDLARYIDHTLLKPEATASDIDTLCDEAREYGFAAVCVNPF
                     WVKRAAARLKGTDVRVASVVGFPFGATPGEIKALEARRAIRDGAREIDMVMNVGALKS
                     GLHDVVRQDVAKVADACHEAGAALKVIIEAALLTDEEKIVAAHLAKEARADFVKTSTG
                     FGPGGATVEDVLLIRETVGPTLGVKAAGGVRTAEDVRDMIAAGATRIGASAGVAIVSG
                     GTGDEPY"
     gene            complement(1648..2241)
                     /gene="rpiB"
                     /locus_tag="VF058_02600"
     CDS             complement(1648..2241)
                     /gene="rpiB"
                     /locus_tag="VF058_02600"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX6207227.1"
                     /translation="MSERRSPEEVRAVVATVVDAVAGEVYEEPRAAEAAAPSPEGTPS
                     LADVVVGADHGGFHLKQRIAAELRDHGFSVQDCGTEGSDSVDYPDFAHEVARLVAKGA
                     CRWGIVVDGAGIGSAITANKVPGVRAATCWDVSSARNSREHNHANVLALGAGLVGEAL
                     ALQIVQAWLSTGWGGGRHARRVSKIDKIERTYARATA"
BASE COUNT          310 a          934 c          763 g          376 t
ORIGIN      
        1 cgcacgacgg cggtcacgta cccgccgccc gtctcctcga aggagaccag ctcgaccttc
       61 gccgccttca ccatcgcgtc ggcggcctcg accatcgccg gaaagctccg gcactcgatc
      121 atcccaagag cctctgccat gtctcctcct cctacctcgt cgcctctcgt ccctcgagcc
      181 cctcgagcgc ggcgacggcc gcctgccatc ccacgtcgat gtctcgctcc tcgcccccga
      241 ggtagatgcg gcccatggag ccgaacgacc gcacctcgag gatgttgatc tccgccgcct
      301 tctcagcctc gttcgccgcg agcgccgcgt aggcggccgg ctcgcactcc agcacgtaca
      361 gcgtctgccc ggggatcagc atctcgccgt ggcggacccg gttgatcagc tgggcctggt
      421 ggtcgtcgat ccggcggatg atctggctgg agaggacccg cggcttgatg cggtcctcct
      481 ccttcagctc gatcgcgtcg aggatcgcgg cgccagcggc ccgggcctcg gcctgctcgg
      541 gcgagtgcac ctcgagcatc ccgaagtacc gctccacgat ctgcatcccc ggcttcaccg
      601 tcgtggactt caacgccacg tcggtgagcc ggttgatctc gatgcccggg gccacctcga
      661 cgtacacgga ggcgtcgccg gccagcggca ggaagccctg cgccaccgtc cccaggaacg
      721 ctgcgtactg cggctggagg ctgtccagga agcagtacgc ccggagctcg atctccttgg
      781 ccatcattcg tcccttcgct cagtacggtt cgtcaccggt gcctccgctc acgatcgcca
      841 cgccggccga cgcgccgatg cgggtggccc ccgccgcgat catgtcgcgc acatcctcgg
      901 ccgtccggac gccccccgcg gccttcacgc cgagggtcgg cccgacggtc tctcggatca
      961 gcaggacgtc ctcgaccgtg gccccgccgg gaccgaaccc cgtcgaggtc ttcacgaagt
     1021 cggccctggc ctccttcgcc aggtgcgcgg cgacgatctt ctcctcgtcg gtgagcaggg
     1081 cggcctcgat gatcaccttg agggccgctc ctgcctcgtg gcaggcgtcg gcgaccttcg
     1141 ccacatcctg ccggacgacg tcgtggaggc cgctcttcag cgcccccacg ttcatcacca
     1201 tgtcgatctc gcgggccccg tcgcggatcg ctcggcgcgc ctccagggcc ttgatctctc
     1261 ccggcgtggc cccgaagggg aaccccacca ccgaggccac gcgcacgtcc gtgcccttca
     1321 ggcgcgcggc cgcccgcttc acccagaacg ggttcacaca cacggcggcg aacccgtact
     1381 cgcgcgcctc gtcgcagagc gtgtcgatgt cggacgccgt ggcctcgggc ttcaggagcg
     1441 tgtggtcgat gtagcgagcc agatccgagg ggacgctgga gccgttcccc gtgtagctga
     1501 tgcggtgggc gccgttgtcg acgatggccc ggaccttgtc cgggacgtgg gccgcgcagg
     1561 agccgcggca gtcggggcag cgctcctccg gggccagcgc ggccagcact tcgctggtga
     1621 cggcctcgat caggtcctca cgcacgctca tgccgtcgcc ctcgcgtagg tccgctcgat
     1681 cttgtcgatc ttcgacacgc gccgcgcgtg ccggccgccg ccccagcccg tggagagcca
     1741 ggcctgcacg atctgcaggg ccagcgcctc gcccacgagc ccggcgccga gcgccaagac
     1801 gttcgcgtgg ttgtgctcgc ggctgttccg ggcggacgag acgtcccagc aggtggcggc
     1861 gcgcacgccg gggaccttgt tggccgtgat cgcggagccg atcccggcgc cgtccacaac
     1921 gatgccccac cggcacgcgc ccttggccac cagccgcgcc acctcgtgcg cgaagtcggg
     1981 gtagtccacc gagtcggatc cctccgtccc gcagtcctgg acgctgaacc catggtccct
     2041 cagctcggcc gcgatccgct gcttcaggtg gaagcccccg tggtcggcgc cgacgaccac
     2101 gtcggccaag gacggggtcc cctcgggcga cggagcggcc gcctcggccg cgcgcggctc
     2161 ctcgtagacc tcgcccgcca ccgcgtccac caccgtggcc acgaccgccc ggacctcctc
     2221 ggggcttcgc cgctcgctca cgacgtccct cccggacggt gcatctcgac ccggtactcg
     2281 tacctgcctc cggcgaacag aaggcgcgtc cactcgatcg gccggtcctg gggcgtgaac
     2341 gtgatccggt ggatcacgag gagcgggctc ccctcctcgc acc
//
LOCUS       DASUTW010000078         3104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1707316, whole genome shotgun sequence.
ACCESSION   DASUTW010000078 DASUTW010000000
VERSION     DASUTW010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707316"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1538
                     /locus_tag="VF058_02605"
     CDS             <1..1538
                     /locus_tag="VF058_02605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6207228.1"
                     /translation="IPPGAALGFPRMRILVWHGWILEGSGSNVYTARIVEAWRAEGHD
                     VLLVAQERHPERFAFLDAWADGSLAEARALDAEPSAGRATLLRPAIGELLPVFVWDEY
                     EGFRVKTFLDLTDDELEGYLDRNVTALREAAEAHGTDVAIAGHAIPGPVVVRRALGSI
                     PFAAKIHGSDVEYAMRGQARYVELAREGLEGAAVIAGASRDVLDRLVELVPSASGRTR
                     VVPPGVEVERFHPAPRAEALRFAADLLERDPALARGRPRTAASDVAEALRRRDGEALD
                     RLANAYEQTSPDRDAPQRLRALAGYDGPLVGYLGKLIPQKGVHHLLAALALQPSRPRA
                     LVVGFGTFREWLEALAAALDAGDPEAAAFLEERMPLGLSPGEVAAAAGPAERVVFTGR
                     LDHRYAGPAAAALDVLVVPSILEEAFGMVALEGAAAGALPLVARHSGLAEVAEALETE
                     IGRPGLLSYEPGPSPPRALARALEELLALPDRRELAARLSSHVRERWSWERTADLLLE
                     PLR"
     gene            complement(1535..2581)
                     /locus_tag="VF058_02610"
     CDS             complement(1535..2581)
                     /locus_tag="VF058_02610"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX6207229.1"
                     /translation="MLERLRAWTYVRQRLDRPARGALEALRAVVAVYATHPTAPLALR
                     ARARSLTGAAYRRLDRERRGLRIPGMRGTVFLVPREAAARIFTAVRPSRSRVVARLKR
                     HDMSEEEYEAIARRVVAVAGEPTRADDLREATGLEGPVLATIVRGLRLEGRLLALAGR
                     PLTSSPHRYVATEAWAEEVLDAGDRESSLAWLGGAYLEAYGPARIEDFAWWAGVGKRA
                     AATAVGEHDTIDVGDGLLLPARHEAAFERSRPLNGTLALLPRWDAYTMGHAPDGRARF
                     VHPDVQPRVYEPIGVGLPGDGNPVVLVDGEVVATWTYSLKDGADVQPFDTLGPGTRKK
                     VEEELDAIAAFLAT"
     gene            complement(2582..2854)
                     /locus_tag="VF058_02615"
     CDS             complement(2582..2854)
                     /locus_tag="VF058_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019158379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HEX6207230.1"
                     /translation="MNETALGVVAASWGVVMALSPVLQIRRILAYRSSRDVSIGYLTV
                     IAIGFALWIVYGLAIENVALIVPNTLAFIVGVIVIGVALRFRSPED"
BASE COUNT          411 a         1166 c         1126 g          401 t
ORIGIN      
        1 ccatcccgcc gggcgccgcg ctaggcttcc cccgcatgcg catcctcgtc tggcacggct
       61 ggatcctcga gggatcgggc tccaacgtct acacggcgag gatcgtggag gcgtggcgag
      121 cggaggggca cgacgtcctc ctcgttgccc aggagcggca cccggagcga ttcgccttcc
      181 tcgacgcgtg ggccgacggc agcctggccg aggctcgggc cctggacgca gagccctcag
      241 ccggacgcgc caccctcctc cgcccggcga tcggcgagct cctcccggtg ttcgtctggg
      301 acgagtacga ggggttccgc gtgaagacct tcctggacct gaccgatgac gagctcgagg
      361 gctacctcga ccggaacgtg acggcccttc gcgaggcggc ggaggcccac ggcacggacg
      421 tggcgatcgc cgggcatgcg atcccgggcc cggtcgtggt ccggcgcgcc ctcggctcga
      481 tcccgttcgc cgccaagatc cacgggagcg acgtggagta cgcgatgcgc ggccaggccc
      541 ggtacgtgga gctcgctcgc gagggcctgg agggcgcggc ggtgatcgcc ggcgcctcac
      601 gcgacgtgct cgaccgcctc gtcgagctcg tcccctcggc gagcggccgg accagagtcg
      661 tcccccccgg cgtggaggtc gagcggttcc atcccgcgcc gcgggcggag gcgctgcgct
      721 tcgcggccga tctgctggag cgagacccgg ccctggcacg gggtcggccg aggaccgccg
      781 cgagcgacgt cgccgaggcg ctccgacgac gagacggaga ggccctggac cggctggcga
      841 acgcctacga gcagacctcc cccgaccggg acgcccccca gcggctccgg gccctcgccg
      901 ggtacgacgg cccgctcgtc ggctacctgg ggaagctgat cccacagaag ggcgtacacc
      961 acctgctcgc cgccctggcg ctccagccct cgaggccgag ggcgctcgtc gtcgggttcg
     1021 ggacgttccg ggagtggctc gaggctctcg ccgctgcgct cgacgcgggc gacccggagg
     1081 cggcggcgtt cctggaggag cggatgcccc tcggcctctc gcccggggag gtggccgcgg
     1141 cggccggccc ggccgagcgg gtcgtgttca cgggacgcct cgaccaccgc tacgcggggc
     1201 cggccgccgc ggccctcgac gtcctcgtcg tcccgtcgat cctggaggag gcgttcggga
     1261 tggtggccct ggagggggcg gcggccggcg ccctcccgct cgtggcccgg cacagcgggc
     1321 tggccgaggt ggccgaggcc ctagagaccg agatcggccg gccgggcctc ctctcctatg
     1381 agccgggacc ctcgcccccc cgagccctgg cccgggctct ggaggagctc ctggccctcc
     1441 ccgaccggcg tgagctggcc gctcgcctct cgtcccacgt ccgggagcgg tggagctggg
     1501 agcgcaccgc cgacctcctg ctggagcccc tccgctaggt ggcgaggaag gccgcgatcg
     1561 cgtcgagctc ctcctcgacc ttcttccggg tcccgggacc aagcgtgtcg aacggctgga
     1621 catcggcccc gtccttcagc gagtaggtcc aggtggccac gacctcgccg tccacgagca
     1681 cgaccgggtt gccgtcgccg ggcaggccca ccccgatcgg ctcgtacacc cgcggctgaa
     1741 cgtccggatg cacgaaccgg gcccggccgt cgggcgcgtg ccccatcgtg tacgcgtccc
     1801 acctcgggag cagggcgagg gtcccgttca ggggccggct gcgctcgaag gccgcctcgt
     1861 ggcgagccgg cagcagcaga ccgtcgccga cgtcgatcgt gtcgtgctct ccgaccgcgg
     1921 tggctgcggc ccgcttcccc acgcccgccc accacgcgaa gtcctcgatc cgggcggggc
     1981 cgtaggcctc gaggtaggcg ccgcccagcc aggccagcga cgactcccgg tcccccgcgt
     2041 cgagcacctc ctcggcccag gcctcggtcg cgacgtaccg gtggggactc gaggtcaggg
     2101 gccggccggc cagcgcgagc agccgcccct cgaggcgcag gccgcggacg atcgtcgcca
     2161 ggaccgggcc ctccagcccc gtcgcctcac ggaggtcgtc cgccctcgtc ggctcgccgg
     2221 ccacggccac cacccgtcgg gcgatcgcct cgtattcctc ctccgacatg tcgtgccgct
     2281 tcagcctggc cacgaccctc gaccgggaag gtcggacggc ggtgaagatg cgagccgcgg
     2341 cctcgcgcgg gacgaggaag acggtgcccc gcatcccggg gatccgcagg cctcgccgct
     2401 cgcggtccag ccggcggtac gcggcgcccg tgagggaccg cgcccgggcc cggagggcca
     2461 gaggggcggt ggggtgcgtg gcatagacgg cgaccacggc cctcagggcc tcgagggccc
     2521 ctcgagcggg gcggtccagg cgctggcgca cgtaggtcca cgctcgcagg cgctccagca
     2581 ctcagtcctc cggagagcgg aaccgcaggg ccaccccgat cacgatcacc ccaacgatga
     2641 acgcgagcgt gttcggcacg atcagggcca cgttctcgat ggcgagcccg tagacgatcc
     2701 agagggcgaa gccgatcgcg atgacggtca ggtacccgat ggagacgtcc ctcgacgagc
     2761 ggtaggcgag gatccggcga atctgcagaa cgggggagag cgccatcacg accccccacg
     2821 acgcagcgac gaccccgagc gcggtctcgt tcacgatgaa cctcctgtgc aaacgacggt
     2881 gcgctcggta cgagggctgc ggtcgctgcc gtgggcagga ggagcgcacc cgcagccgtt
     2941 tcgagtctac caacgtgagc gctgctcgag aaagagtgac aagacgagcg tcccgggttg
     3001 cgcccgcacg tccgggcggt gtacaaacgc ttcggaaccg ctggggtctt cgggccccgg
     3061 cggttccgtc atctccgccc gactttcctc caccggtcct gctg
//
LOCUS       DASUTW010000079        12511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1837820, whole genome shotgun sequence.
ACCESSION   DASUTW010000079 DASUTW010000000
VERSION     DASUTW010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12511
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1837820"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VF058_02620"
     CDS             complement(<1..563)
                     /locus_tag="VF058_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207231.1"
                     /translation="MKHPRKTQGLGLVLFGLLLGMFLFTPATAHYTTSTKHLGQHAWN
                     QVIKAKVFTKKQANDRFALKKAEAYRLVGTTGQPPFQNGWGNFGFGWSATGFYKDSEN
                     LVHLKGTFEGPSGFTAFTLPVGYRPPENLLLPIAGIGNDGLRIFVDGTVVPVCATSSC
                     IAGIDGLTFRVGPGAGAAAAASDSTGTG"
     gene            complement(1050..1589)
                     /locus_tag="VF058_02625"
     CDS             complement(1050..1589)
                     /locus_tag="VF058_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207232.1"
                     /translation="MALVLIGVLTGMLLITPSTAHFSQNTKHLGSHAWKQVIKQKVYT
                     KQQANAIAPRVTRASRSTTLSLTTADQIYISQDVTAPTRGYVLLNATVTFQAESACTG
                     ACGMAGFIQHAQTFATSIGATTNLHTAGDRASIALTTVMAVSKGSNTFNVLVARGANG
                     AVLAVRGEMTALFSRFGGA"
     gene            2458..3018
                     /locus_tag="VF058_02630"
     CDS             2458..3018
                     /locus_tag="VF058_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207233.1"
                     /translation="MSGSDHGRGRPLGRLLLSGVAVVALTAAACADGSSGSQQGGTGD
                     AGTGGESGDLTIVMDEYAFIPDTLTVSAGDEIEVTIVNDGNEPHELMIGMPTGGGPEW
                     GTDLFGMMNPEVMSGEGYHMEGVGHMEGEEGEHGMGHGAEVELEPGGQVTLRMHVPED
                     ATGEWEMGCYLGGHYEAGMHGTMVVQ"
     gene            3537..3824
                     /locus_tag="VF058_02635"
     CDS             3537..3824
                     /locus_tag="VF058_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207234.1"
                     /translation="MHELRVGQTLLPGGGYEEDLLAMMEPQVLSGTGYRLLGPEEDAA
                     GGPGGLSVEVEPGASVTLRLSVPANAAGTWEMGCFITGHYEAGMKAELRLE"
     gene            complement(4653..5384)
                     /locus_tag="VF058_02640"
     CDS             complement(4653..5384)
                     /locus_tag="VF058_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207235.1"
                     /translation="MMRSGFSRVGVVLALLIGVLVTPGAQSVSAHRTQLAVEVLSGQV
                     ALFPDGRSVLVHLKTQCDPKWTIVDARVRVEQGEAWGERSFTPRCQRLSYGLQVTVPT
                     IVGTFRTGPAQSSAVLVVQQGKAKEARASATLDLRPRVSIQLGGTAALEDGGGAVRID
                     VTVTCPEVSTGLGGQVRVYDGQVAGTGTFGATPCDTTPHTVSVRVETTEGTFRVGSAE
                     AFAFVGIEERGDIFQDADLRTVEVI"
     gene            5667..7331
                     /locus_tag="VF058_02645"
     CDS             5667..7331
                     /locus_tag="VF058_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6207236.1"
                     /translation="MALVAVIVMAAVSVGGSGSLAKRKAKKPLYTKKQTNTRYYTKDK
                     ADARFADTVVRDELVVAVPSGPPLQSITIDDLPNLRASSAVTLILLVAATRLVSAWGR
                     WEGTGMRPETRYAKSSGGYVAYQVFGDGPRDILFVPSWSQNLDAMWDEPTLARYFQRL
                     SRAGRVICFDKRGSGVSDPVPLANIPTLEEWMDDAVVAMDAAGSEQAAVIGDAEGGPM
                     AMLLAATVPDRVIALALVNTFARWSRAPDYPIGMPESAFEHLMAIYEQTWGQNADMLL
                     LTAPSVADDPRIRDWFVRYQRLSMPPGAATATYRWVSSLDVRAVLPTISAPTLVVHRT
                     ENRHYRLAFGRYLAEHIPEARLVELPGADCYPFHVGDSEAVLDEIHAFLTGVREGPPA
                     ERELATVLFTDIVGSTDLAAELGDARWLDLRDDHDTVVRRIIAAYRGQEVTTTGDGFL
                     VTFDGPARAVQCAVRAGEAVRSLGLEIRAGLHTGEIERRDGDVGGLAVHLAARVMGEA
                     GAGDVLVSGTVRDLVVGSGIDFEDRGEHSLKGVPGTWRLLRVTGVP"
     gene            complement(7395..7706)
                     /locus_tag="VF058_02650"
     CDS             complement(7395..7706)
                     /locus_tag="VF058_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207237.1"
                     /translation="MGPGGPELANGGGTIRPEFVWAALDCPGAFATGFPGAPMVVGRL
                     AARVVRSARVGERCVVMGWSEGSEGRKRFAGTAVLGEDGEPLATAMATWIELQPEARF
                     S"
     gene            8394..9290
                     /locus_tag="VF058_02655"
     CDS             8394..9290
                     /locus_tag="VF058_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207238.1"
                     /translation="MHALTRSRRVATIVGAAAALMVLGQAAQAVPVYLEGNDDTLLVG
                     GKLSRWDVYNNTSACESDSPGYTPADDGELGTEGSSDAFDGGLLFMVGPANGVVDDAA
                     TFFDGNSIGSKTGEQIVVGPKLTGGLKVKRWDRALPKSPTMRSLIALRNTSNSPRTRD
                     VYLDTNLGSDGLTDIRRTSNGSANLLNASRWVVTSDSATDPGDPVVTHVLWGKRARSK
                     TAEVVNNPGAGAPADCLTVRMRVRVPKKSVRYLLFFAEMNPTNAGGISSAAKFNKRTL
                     NKRLLAGIGPGVRNKILNWRLG"
     gene            complement(9371..9976)
                     /locus_tag="VF058_02660"
     CDS             complement(9371..9976)
                     /locus_tag="VF058_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207239.1"
                     /translation="MVRRAGAAVLLGILVAIPVAAVGWTWLATPDHVAEVRALEDRAI
                     AYMERFRAPELEFRGTYDRVPESDVRLVTALRRLPGGELLAFAMWGDSGPPWEPGYGV
                     WVRARYRSSLSERETCHLLASTAGVPRSECVAGEGDPLVPDERPYTLSLRGEGLEGSA
                     EVYRRRLRDANGDLEPEFSVVADIVMRACGTERFPGIGSCP"
     gene            complement(9976..10776)
                     /locus_tag="VF058_02665"
     CDS             complement(9976..10776)
                     /locus_tag="VF058_02665"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEX6207240.1"
                     /translation="MRARRASIAFVLLLVLGACGDEASRPRQGPSPSPTVGVEAEGSW
                     QLKSGTLRGDPVPIVPDHRIMLTIEGREFGGRAACNLYGGTIRIEGDAVALTEIAQTE
                     MGCPPPIMASEKAYMAALPLVDTVRREGSILTLSGPETILRFVELPDVDDDLFGTLWV
                     LDALIEGDIVTSVGGERATLRLDSGRLEGTTGCRRLTGRYVERGDVIFATALSAHGRC
                     PADLSDQDGYVVGVLGDGFTATVEGDRLTLTSMGGIGLIYRAASAGEE"
     gene            10943..11380
                     /locus_tag="VF058_02670"
     CDS             10943..11380
                     /locus_tag="VF058_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207241.1"
                     /translation="MAKRLLLLGAALTVLLAPAGVARAGVTGPAFYVDGQLYRTVGTP
                     TDLSQTGAPDHSFDTIYEFFGVQTNVATAAPGDREYNGGRWRVIGVEFSSYEDAVAGA
                     DTNGSGDLDTTAEVEAALAGPHAESIGVVKQFVCPVIPLPRSG"
     gene            complement(11449..11706)
                     /locus_tag="VF058_02675"
     CDS             complement(11449..11706)
                     /locus_tag="VF058_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEX6207242.1"
                     /translation="MQMRPLDWLAGILLVIGGLNWGLVAIAEFDLVAWIVGLEFGAVN
                     VASRLIYGVVALAAVYFAVRLPMILTGRQTIATRHELRPAA"
     gene            complement(11903..12277)
                     /locus_tag="VF058_02680"
     CDS             complement(11903..12277)
                     /locus_tag="VF058_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207243.1"
                     /translation="MTRSFTRWLAVLGLSLALLAGAGSAVARPLMDATECRLPGQLLR
                     LETELGESCRGAVTTYRGELREAEMPPTSSGSSAWIVWTGASALLLLAGSGLGVALFR
                     IRARAASSEGLDDGLDRLALGR"
BASE COUNT         2013 a         4294 c         4345 g         1859 t
ORIGIN      
        1 ccagtgcccg tcgaatctga ggctgcggca gcagcgccag ctcccggacc aaccctgaat
       61 gtgaggccgt cgatgcccgc gatacaggac gacgtcgcac agacgggaac cacggtaccg
      121 tctacaaata tgcgcagtcc atcgtttcca attcccgcga tcggcagtag aagattctcc
      181 ggcggacggt atcccactgg gagcgtgaag gccgtgaaac cgcttggccc ttcaaacgtc
      241 cctttaagat gaaccaggtt ctccgagtct ttgtagaagc cggtggcgct ccagccgaag
      301 ccgaagttcc cccaaccgtt ctggaagggc ggctgccctg tagtcccgac cagccggtat
      361 gcttccgcct tcttcaaggc gaaccgatcg ttggcctgct tcttcgtgaa tactttcgcc
      421 ttgatgacct ggttccaggc gtgctggcca aggtgcttcg tgctcgtcgt gtagtgggcg
      481 gtggcggggg tgaacaggaa catccccagc agcaacccga agaggacgag gcctagcccc
      541 tgtgttttcc gtggatgttt catcctgtgc tccctccgag tcgttttccg aggcgagatc
      601 cgaggtcgac cgcgtcggct gatccttgca ggatgcctcc cctccgcctc agagaagcgt
      661 tcggagggag aaggatgctc gaagcgtgtt ggtatgagac agggtcgaac ggccccaatc
      721 gggcgggacc tcagggcgac cgacgggctc cccgctggaa ctagatgagg atatggaggc
      781 gggcgagccc ttcgttgcag gtagcagggg cccttatccc cttgctcagc cagtccacta
      841 ccgccaggat cgacatccac acgccgaacc ccagaagcat cgcgatcggt cgcggcggat
      901 cgcaagcgga cgatcgcaac ctcctgtggg ggcgaactga ccggactgcg gaccggacgg
      961 cgtcgagctc cgtcccaggg agcacgaagg cctgaacccg ccgggcctgg atcttgcggt
     1021 ggtccgcctg cggccgggcc cggtgagctc taggccccgc cgaaccgcga gaagagcgcg
     1081 gtcatctccc cacgcacggc gaggaccgcg ccgttggcgc ctcgggccac gaggacgttg
     1141 aacgtgttcg atcccttgct caccgccatg acggtggtga gggcgatgct cgcgcgatcg
     1201 ccagccgtgt gaaggttcgt cgtggcaccg atcgacgtgg cgaaggtctg agcgtgctgg
     1261 atgaagccag ccatcccgca ggctccggtg cacgccgatt cagcctgaaa cgtgacggtc
     1321 gcattgagca agacgtagcc gcgcgtcggc gctgtgacgt cctggctgat gtagatctgg
     1381 tccgctgtcg tcagagacag ggtggtgctc ctcgacgccc gtgtaacccg cggggcgatc
     1441 gcattggcct gctgcttcgt gtagaccttc tgcttgatga cctgtttcca agcatggctg
     1501 ccgagatgct tggtgttctg gctgaagtgg gccgttgacg gggtgatgag cagcatcccg
     1561 gtcagtaccc cgatcagtac cagcgccatc cctgaagcct tgggtgcaga gcccgagcgc
     1621 atgtctgcct ccttcgttcg tgccgcgcgc cagtcgcctc caccggcgac gacggccaac
     1681 ccccggcacc cgggcaagcg gcccttcggc tagagcatgg ggacggcgcg tgccggcgac
     1741 ctagggccga tcggcctccc gtggatgggt cgcccgtcct ccgctcgtgt cctgatccgg
     1801 gtatggaggg caagcgctcc ggtgctctcc gaggacctac gcgcccttcc gccaccacta
     1861 ggtgttgcca gagcagggag ctaccatccc tctgaggggg gaatggggct tcgagggagg
     1921 cagcaagtga cggacaaggg gaagaggctc gcgatctacg tagggctccc ctcgcccgat
     1981 gaagtgcaaa gggaggccga actcgccgca ctggacggtg tggagggagg aggcttgagg
     2041 ccgcacccac ttgctcgacc ggacttccca tggcccgatt tcgcggacgt ccgatggcgg
     2101 ggcatgtaca cgggtgacgc cctattgcgc tgatcccgcc gcgaggaggt caatccactc
     2161 ttgggcagcg tgccctcaaa ggcttcacgg gttccgccgc caagggacga cgtcgaccgg
     2221 atcccggagg cacggaggac gggaagcccc aggaggggta tctgttccgc ggacactcct
     2281 cgatctgcga actctcgtct tcgcgggcgt cttcttgctg ctggagcggg gacgtttcgg
     2341 cccgtaggtg ggcgacggac gagtcgaacg gcccgatggc ggcgggccct ccggccctcc
     2401 tccgtgatcc gggcgggacc catcctcgtg aattgaaccg tccgaaagga gagcgagatg
     2461 tccggatccg accatggacg gggccgcccg ctggggcgcc tcctgctgag cggggtcgcc
     2521 gtcgtcgcgc tgacggccgc cgcctgtgcc gacggatcct ccggctcaca gcagggcgga
     2581 acgggtgatg cgggcacggg tggggagtcg ggtgacctga cgatcgtcat ggacgagtac
     2641 gcgttcatac ccgacacgct gaccgtctcc gccggcgacg agatcgaggt cacgatcgtg
     2701 aacgacggca acgagcccca cgagctgatg atcgggatgc ccaccggcgg cggtcccgag
     2761 tgggggaccg acctgttcgg catgatgaac ccggaggtca tgagcggcga gggctaccac
     2821 atggagggcg tcggccacat ggagggcgag gagggcgagc acggcatggg ccacggcgcc
     2881 gaggtcgagc tggagcccgg cggccaggtg accctgcgga tgcacgtccc cgaggacgcc
     2941 accggcgagt gggagatggg ctgctacctg ggcgggcact acgaggcggg catgcacggc
     3001 acgatggtcg tgcagtgacg aaccggagtc agcgcggcgg agccctcgcc gcggggtcgg
     3061 atccggtcgg catcggtttc gccgcgttct cggccgcggc ggatccggtg gggtcgcggt
     3121 ggcatcgagg tcgaagtcgg gaccggtctc agagtcgggt caggtcgggg gggtaccgag
     3181 tcattccgct ctacggactt cggccttcga ttggctcccc tgatggatgc taccctggtg
     3241 cggtgccggg gggatgggcg agagggtgga ccagacctgc ctccctgatc ggggtggtgg
     3301 cgttgttggc cgggctggtc acaagctgcg actctgccgg tcccaccccg acggcaagct
     3361 cgactgaaga gcctcagcag acggaatcgc cggcggcaca gccgctggtg cttctgatgg
     3421 acgaattcag cttcaccgtc gagggccctt ctcagcccgc aggctacccg cgaccgaccg
     3481 ccgctttacc gaccgatagc tcgggcgtcg ttacgctgca aaacgatggg cgcatcgtgc
     3541 acgagctgag ggttggccaa accctcctcc caggcggggg gtacgaggag gacctgttgg
     3601 cgatgatgga gccgcaggtc ctgagcggca cagggtaccg gctactgggg cccgaagaag
     3661 acgccgcagg ggggccgggc ggcctcagcg tcgaggtaga accgggcgcc tccgtaaccc
     3721 ttcggctgtc cgtcccggcc aacgcggccg gtacgtggga gatgggctgt ttcatcactg
     3781 gacactacga ggccggcatg aaggcagagc tgcgccttga gtagtacggt tctgaccctg
     3841 ccggaggcca ctcaggcgat ccacgggcca atcgccgagc cggggtcgga gccgaccgac
     3901 gagccggagc cgaccggcgc cgcggaggcg ggtcgggatc ggggaccgag acgggagtcc
     3961 ggatcggggg gctaagaggg gatcgccgtc accgcccggg aagatgttct tgacgaaggt
     4021 ggtcccggcc ggcctcccgg tcgtgcgata gagcttccgg ttggtggtcc gtcgtcggcg
     4081 ccgaagtaga ggtagtcgcc aaggcgctcg tagaagcccg ggtgggcgtc acccactccc
     4141 ggattgatgt ccttcacgcg gcgggtgccc ttgcgggtcc cgttcgtccg ccacagctcg
     4201 cgacccatcg aggcgttcgt ctccgtgaat accacccgct tcttcagagg cgtgaactcg
     4261 gcgggaaagg agtcggcggc gccgggttga tgtccttcac ccgccgggtg cccttacggg
     4321 tgcccttcgt ccgccagagt tcgaaccccg aggatccgtt gtcggcggag aagaggatcc
     4381 gcttgccgac gcgggcgagc ggaagcgcgg agggaggctt cactcgggaa acctcatccc
     4441 ctatccgatc aaccgcgatc agggtagggg gctcggcgat ccaaccaacg accggcagcg
     4501 agtccggatc ggctcgcctc gactctggct agccggtcgg gtcggatccg gtcgcgcccc
     4561 cgaccccgac tccgcgaccg acccccaacg ctgaccccgc gcccgagttc gagtcgaggt
     4621 cggtggcagc gacttagctc ggggtcaatg catcagatca cctcgacggt tcggagatcg
     4681 gcgtcctgga agatgtcgcc ccgttcctcg atgcccacga aggcgaacgc ctcggcgctc
     4741 cccacccgga acgttccctc ggttgtctcc accctgacgc tcacggtatg gggggtcgtg
     4801 tcgcacggag tggccccgaa ggttcccgtg ccggcgacct gcccgtcgta gaccctgacc
     4861 tgtcctccca gccctgtgct gacctcgggg cacgtcaccg tcacgtcgat gcgcacggcc
     4921 ccgccgccgt cctccagcgc ggccgtcccg ccgagttgga tcgagacgcg cggcctgagg
     4981 tcgagggtgg cggaagcccg ggcctccttc gcctttccct gctgcacgac gagtaccgcg
     5041 ctcgactggg cggggcccgt ccgaaacgtc cccacgatcg tcggcacggt cacctggagg
     5101 ccatagctga gacgctggca gcgcggcgtg aacgatctct ccccccaggc ctccccctgc
     5161 tccacgcgaa cccgggcgtc cacgatggtc catttcggat cacactgcgt cttcagatgg
     5221 accagaaccg accgaccgtc cgggaagagt gcgacctgac ccgacagcac ctcgaccgcc
     5281 agctgggttc ggtgcgcgct caccgactgc gctcccgggg tgacgagtac cccgatgagc
     5341 agggcgagga cgacacctac cctgctgaac ccgcttcgca tcacgaacca cctcctcgtg
     5401 tcgtcgggag aactactggt ccaccgcccg ggacggattg cctgccgatc ggcggtctct
     5461 gcggtcgacg catcggacag ctctgaggcc cctcaccagg caccctcagc cgtggattgg
     5521 gaggcgaagc gtggcacggc tggcagcgcg catgccttgg gagcacgagg ccggcggttc
     5581 gaatccgctc gccccgacgg cttcgtcgga aaggagcgcg ccatgagtcg cagccgtcag
     5641 ctgctccgtc tctctccgtt ctcgccatgg ctctcgtcgc ggtgatcgtc atggctgcgg
     5701 tctccgtggg cgggtccgga tccctcgcca agcggaaggc gaagaagccc ctctacacga
     5761 agaagcagac caacacccgc tactacacga aggacaaagc ggatgcccgc ttcgcggaca
     5821 cggtggtgcg cgacgagctg gtggtggccg tcccctcggg cccaccgctc cagtcgatca
     5881 cgatcgacga cctcccgaat ctcagagcgt catcggcggt gacgctgatc ctgttggtag
     5941 cggccacgcg gctcgtgtca gcatggggac gctgggaagg gaccgggatg cgtccggaga
     6001 cgaggtacgc gaagtcgagt ggaggctacg tcgcctacca ggtcttcggc gacgggccac
     6061 gggacatcct cttcgtcccg agctggagcc agaacctcga cgcgatgtgg gacgagccca
     6121 cgctcgcccg ctacttccag cggctgagcc gggccgggcg cgtgatctgt ttcgacaagc
     6181 gcggctccgg ggtctccgac ccggtccccc tcgcgaacat ccccacgctg gaggagtgga
     6241 tggacgacgc ggtcgtggcc atggacgccg ccgggtccga gcaggcggcg gtcatcggcg
     6301 acgcagaggg tgggccgatg gccatgctgc tcgccgcgac ggtccccgac cgcgtcatcg
     6361 cgctcgcgct cgtgaacacc ttcgcccgct ggtcccgcgc ccccgactac cccatcggga
     6421 tgcccgagag cgccttcgag cacctcatgg ccatctacga acagacctgg gggcagaacg
     6481 cggacatgct cctgctcacc gcgccgagcg tcgccgacga cccccgcatc cgggactggt
     6541 tcgtcaggta ccagcggctg tcgatgcccc cgggagccgc gactgccacg taccgctggg
     6601 tgtcctcgct cgacgtacgg gcggtcctcc ccaccatctc ggcccccacc ctcgtggtgc
     6661 accggacgga gaatcgccac tatcggctcg ccttcgggag gtacctggcc gagcacatcc
     6721 cggaggcccg gctcgtggag ctgccgggcg cggactgcta cccgttccac gtcggagaca
     6781 gcgaggcggt cctcgacgag atccacgcgt tcctcaccgg ggttcgggag ggtccgcccg
     6841 ccgagcggga gctggccacg gtcctgttca ccgacatcgt gggctcgacc gacctggccg
     6901 cggagctcgg ggacgcccga tggctcgacc tgcgggacga tcacgacacg gtcgtccggc
     6961 ggatcatcgc ggcctaccga ggccaggagg tcacgaccac cggcgacggc ttcctcgtga
     7021 ccttcgacgg gcccgcgcga gccgtccagt gcgccgtgcg agccggggaa gccgttcgct
     7081 ccctgggact cgagatcagg gcgggcctgc acacgggcga gatcgagcgt cgggacggcg
     7141 acgtcggggg actcgcggtc cacctcgcgg cccgggtgat gggggaggca ggcgcgggcg
     7201 acgtcctcgt ctcggggacg gtgcgtgacc tcgtggtggg gtcggggatc gacttcgaag
     7261 accgaggcga gcacagcctg aagggggtcc cgggaacgtg gcggctcctt cgcgtcacgg
     7321 gcgtcccctg atcagcgagt ggggggccac taggagggtc ttcgagaggc tcaggctgga
     7381 cgagccgacg cggctcagct gaaacgagcc tccggttgga gctcgatcca ggtggccatc
     7441 gccgtcgcta ggggctcgcc gtcctcgccg agcacggcgg taccggcgaa gcgcttccgg
     7501 ccttccgagc cctcggacca tcccatcacg acgcaccgct cgccgacgcg agcggaacgg
     7561 acgaccctcg cggccagccg cccgaccacc atcggcgcgc ccgggaaccc ggtcgcgaac
     7621 gccccgggac agtccagggc cgcccacacg aactccggcc ggatcgtccc tcctccgttg
     7681 gcgagctccg gccccccggg acccacggcg ccgcgacgag gtcgcggccg gggacggggc
     7741 cggggaagat ccgcagcccg tccccttcct cgcgctcggg gccgcagacg aagcagctcg
     7801 ggaagacgtg acgatcgaac cccgcgtagc cgcggctggc gtcctgggcc gtgcgccgat
     7861 cgacgggatc ggggacgtcg acccccagcc gcgtagggag gccgtcggcc accagttccc
     7921 cgtcgtggac gagctcgact ccctcctggg cgggtcgcac atgaaggggc cggcagagtg
     7981 gcgtcggccg tcggagggtc acccgggcgg cgtcgccgag cagcccggcg atgagcccgc
     8041 tgacgtagcc gccgttcccg gacgcgggcg gtccacggaa gcgcgcatcg acgatgaagg
     8101 tctgttcgcc catgaggttg gtccgcttca gagtacgcgg cgcggcggcc accgcccagg
     8161 atccaagcgg tcctccactc cccgcggcga acgcagtccc cgctcaacgt ggatccaatc
     8221 cacacatcgg gacagatctc ctctatggtc tctcacgagg gtggcccgcc cccctcggtc
     8281 gcgatcggcc gtaccggccc cgagccgacg ggaagggagg tgccaagccg cgggacggtt
     8341 tgttcatcgg atccgtcgag gagctgttcg ctgctcggaa ggagagacga agcatgcacg
     8401 cactcacccg aagccgcagg gttgccacga tcgtgggggc cgcggccgcg ttgatggtgc
     8461 tgggccaggc ggcccaagcg gtaccggtgt acctggaggg caacgatgac accctcctcg
     8521 tgggcgggaa gctgtcgcgc tgggacgtct acaacaatac cagcgcgtgc gagtcggatt
     8581 cccccggcta cacgccggcg gacgatggag agctagggac cgagggcagc tctgatgcct
     8641 tcgatggagg ccttctcttc atggtgggtc ccgccaacgg cgtcgtggac gacgccgcca
     8701 ccttcttcga cgggaacagc atcggcagca agacgggcga acagatcgtg gtgggtccga
     8761 agctcacggg gggcctgaaa gtcaagcgct gggatcgggc gctcccgaag agccccacga
     8821 tgcgttcctt gatcgccctg cgcaacacga gcaactcgcc gcggacccgc gacgtctacc
     8881 tggacacgaa cctcggatcc gacgggttga ccgacatccg gaggaccagc aacgggtcgg
     8941 cgaacctcct caacgcctcc cggtgggtgg tcaccagcga cagcgccacc gacccaggcg
     9001 accccgtggt gacacacgtg ctgtggggga agagggcgag gtccaagacc gcggaggtcg
     9061 tgaacaaccc cggcgccgga gcgcccgccg actgcttgac cgttcggatg cgagtgcggg
     9121 tgccgaagaa gagcgtccgg tacctgctct tcttcgctga gatgaacccg acgaacgcgg
     9181 gagggatcag ctccgccgcg aagttcaaca agcggacgct gaacaagcgg ctgctggcgg
     9241 ggatcggtcc cggggtcagg aacaagatcc tcaactggag gctcggctga gtatcgtcct
     9301 cggctagtcg agagggcctc cctccgggga ggccctctcg tcatctctgg accgctcggg
     9361 tccgccgggg ctacgggcag gagccgatgc ccgggaaccg ctctgtgccg catgccctca
     9421 tcacgatgtc ggcgacgacc gagaactcgg gctccaggtc gccgttcgcg tcccgtagcc
     9481 tccgccggta cacctccgcg gacccttcga gcccctcgcc acggagcgac agcgtgtacg
     9541 ggcgctcatc cgggacgagc gggtccccct cgccggccac gcattcggac cgaggcacgc
     9601 ccgcggtgct cgcaaggagg tggcacgtct cccgctcgga gagcgaggaa cggtagcgcg
     9661 ccctgaccca gacgccgtac cccggctccc agggaggccc ggaatccccc cacatcgcga
     9721 aggccaggag ctcgccaccg gggaggcgcc tgagcgctgt gaccagccgg acgtcgctct
     9781 cggggacgcg gtcgtaggtc cctcggaact ccagctcggg ggcccggaat ctctccatgt
     9841 aggcgatggc gcgatcctcg agggctctga cctccgcaac gtggtcgggg gtggccaacc
     9901 aggtccaccc caccgcggca acggggatcg ccacgaggat ccccaggagg accgcagccc
     9961 ccgccctccg caccatcact cctcacccgc cgaggccgcg cggtagatga ggccgatccc
    10021 tcccatcgag gtcagggtga gccgatcacc ctcgaccgtc gccgtgaacc cgtcgcccag
    10081 caccccgacc acatacccgt cttgatccga cagatcggcg gggcatcgcc cgtgcgccga
    10141 cagggcggtg gcgaagatca catcgccccg ctcgacgtac ctcccggtga gccggcggca
    10201 gccggtcgtg ccctcgagac ggcccgagtc cagccgcagg gtcgcccgct cgccgccgac
    10261 ggaggtgacg atgtcgccct cgatcaaggc atcgaggacc cacagcgtgc cgaagaggtc
    10321 atcgtccacg tccggaagct cgacgaaccg caggatcgtc tcgggcccgg acagggtcag
    10381 gatcgatccc tccctccgga cggtgtccac cagcggcagg gcggccatgt acgccttctc
    10441 ggaagccatg atgggcggcg ggcagcccat ctcggtctgt gcgatctccg tgagcgcgac
    10501 cgcatcgccc tcgatcctga tcgtgccgcc gtagaggttg caggcggccc tcccgccgaa
    10561 ctcccggccc tcgatcgtca gcatgatgcg gtgatccggg acgatcggca cgggatcgcc
    10621 tcgcagcgtt cccgacttga gctgccagct gccctcggcc tccacgccca cggtcgggga
    10681 ggggctcggg ccctgccgcg gccgcgaggc ctcgtcgccg caagccccca ggacgaggag
    10741 gaggacgaac gcgatggaag cccgtcgagc cctcacgatc gatgagacgc tcctcgcccg
    10801 tggcgggttc cagcatcggc cgcccgcccg aacgggtcaa gggtcggctc gaccaggcgt
    10861 aagcgtttgg aaaggggccg ggcgccagag tggcctccgg gacgcgtccc ggggtgggac
    10921 gcggcgaacg aaggaggaac ccatggcgaa gagactgctg ctcctcgggg cggcgctcac
    10981 cgtgctcctg gcgccggccg gggtggcgag ggcgggagtg accggccccg cgttctacgt
    11041 ggacggccag ctctatcgaa cggtagggac gcccacggac ctgtcgcaga ccggggcccc
    11101 ggaccacagc ttcgacacga tctacgagtt cttcggggtc cagacgaacg tggcgaccgc
    11161 cgctcccggc gaccgcgagt acaacggcgg gcgctggcgc gtgatcgggg tggagttcag
    11221 ctcctacgag gacgcggtgg cgggggccga cacgaacggc agcggggacc tggacacgac
    11281 cgcggaggtc gaggccgcgc tggccgggcc tcacgccgaa tcgatcggcg tggtgaagca
    11341 gttcgtgtgc ccggtgatcc cgctccctcg ctccggctga gggaagcggg caaggttgga
    11401 agcgcccgcg cgggggatac atcccccgcg cgggcgcccc acgtccccct aagccgccgg
    11461 ccggagctcg tgccgcgtgg cgatcgtctg cctccccgtg aggatcatgg ggagacgcac
    11521 cgcgaagtac accgcggcaa gcgccacgac gccgtagatg aggcgcgaag ccacgttcac
    11581 cgcgccgaac tcgagtccca cgatccaagc aacgaggtcg aactcggcga tggccacgag
    11641 gccccagttg agcccgccga tgacgagaag gatccccgcc agccagtcga gcggacgcat
    11701 ctgcatccct ccttcccgta cgtcctcgat cacagcatgc ccggcgcccg tcgcccggac
    11761 aggggtcaca cggcccccat gtcgagggac ctccggccga agggtgtggg cgcccccctc
    11821 ggccgacgag gtccgatgct cgaaggctgg tccaccgcca ctcaactccc gagggagccc
    11881 cgtcgcggac gagtcggctc gttcatcgtc cgagcgccag acggtcgagg ccgtcgtcga
    11941 gcccctcgct cgaggcggcg cgagcccgga tccggaagag cgccacgccc agacccgagc
    12001 cggcgagcag cagcagcgcg ctggccccgg tccagacgat ccacgcggac gagccggacg
    12061 aggtcggagg catctccgcc tcccggagct caccgcggta cgtggtcacg gccccacggc
    12121 agctctcgcc cagctcggtc tcgagcctca gcagctgtcc cgggagcctg cactccgtcg
    12181 cgtccatcag ggggcgcgcc acggcgctcc ccgcgccggc caagagcgcc aggctgagcc
    12241 cgagcacggc cagccaccgg gtgaaggatc gagtcatctc gtgcctccct tcggttcttc
    12301 cgcgtgcgcc ctgcacgcac ggtcgaacct agggacgcgc cggcgggggc acaccgggag
    12361 ggatgcagat gttgggagcc ggcgatacct atcttgggga ggccgaggcc tcgctggagg
    12421 gctgctcgtc cggagcggcc gtctcgtccg agagcaggtc ctgctcgatc acccaccggg
    12481 cgacctgcgt gcgggacgtg aagccgagct t
//
LOCUS       DASUTW010000080         3112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1449150, whole genome shotgun sequence.
ACCESSION   DASUTW010000080 DASUTW010000000
VERSION     DASUTW010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449150"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1029)
                     /locus_tag="VF058_02685"
     CDS             complement(1..1029)
                     /locus_tag="VF058_02685"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HEX6207244.1"
                     /translation="MALVAGGLAYVQLEKKVTLVVDGAERRVSTFASSVGDLLEGTGI
                     EIGEHDEVSPPLDAPVADDMRVEVLLAKEITLVLNGKPRTLYVTGRTVEDVLSHVNLR
                     SANKAYVRPSRGAAISDGDRIVLREAVEVRVKVDGADRQVITNAPDVGHLLVSLGVIL
                     RKNDEVAPGLDRRLTNGLTIRVTRVTFEQATVREQIPFGTEDRYSDEFLKGVTHVERE
                     GAPGIREKVFRVRLEDGKEAARTLVSERVVEQPVSRIVIHGTRDPNVQTGLASWYHRS
                     GMVAAHRTLPFGTRVKVTNLGNGRSVWVTIDDRGPYIDGRIIDLSDDAFLQLAPLGAG
                     TINVRITW"
     gene            complement(1223..1978)
                     /locus_tag="VF058_02690"
     CDS             complement(1223..1978)
                     /locus_tag="VF058_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX6207245.1"
                     /translation="MIDTHCHLFLMEGEPKELVREAEEAGVGTLVCVGVDRESSHRSR
                     ELADSIRGVFATAGVHPHDAKDFDDATGVEIEELLSDPRVVAVGETGLDHYRMLSPAE
                     DQDRVFRAHCSLAREHDKPVVVHSRDAWPDVLRILEEERLERVILHCFSGGEEEAREA
                     ARRGYHCSFAGPITYPKNERLRAAAAVVPEELLLVETDSPYLPPQPLRGRPNAPGNLR
                     LTLQAVAEVRGQGLEELEEITDRNARAAFALPG"
     gene            complement(1982..>3112)
                     /gene="metG"
                     /locus_tag="VF058_02695"
     CDS             complement(1982..>3112)
                     /gene="metG"
                     /locus_tag="VF058_02695"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX6207246.1"
                     /translation="YTEDELVSGNCPIHGRPVEAMSEENYFFRLSAYADRLLEHYERH
                     PEAVEPETRRNEVLSTIRQGLRDFSISRTSFAWGVPLPWDPSHVCYVWFDALTNYITA
                     AGFADDPERFERMWPADVHFIGKDILRFHAIYWPAMLMAAGVEPPKQVWAHGFLLVGG
                     EKMSKTKLTGIHPFELVDHFGVDAYRWYFLREVAFGQDGSFSWESMVARTNADLANGL
                     GNLASRVLAMIVSSFEGGVPEPASEGLDGGLPDTIDEVVARYDRLVNDMELTAALGAV
                     WEIVGRANEYLVEVEPWKLAKDPDRRDQTATILYTAAETLRILAVLISPVMPSAAGRL
                     WAQLGITEPLEEQRLPEAARWGGLVPGTRVTKGEALFPRLEG"
BASE COUNT          462 a         1107 c         1077 g          466 t
ORIGIN      
        1 ctaccaggtg atccggacgt tgatcgtccc cgcgccgagg ggggcgagct ggaggaaggc
       61 gtcgtcggag aggtcgatga tccgtccgtc gatgtacggt cctcggtcat cgatcgtcac
      121 ccagacgctt ctcccgttgc cgagattggt caccttcacc cgcgtcccga acgggagcgt
      181 ccggtgcgcg gccaccatcc ccgaacggtg gtaccacgag gcgagccccg tctgcacgtt
      241 ggggtcgcgg gtcccgtgga tcacgatccg ggagacgggc tgctccacga cccgctcgga
      301 gacgagcgtg cgagcagcct ccttcccgtc ctccaagcgg acccggaaca ccttctcccg
      361 gatccccggc gctccctcgc gctccacgtg ggtcacgccc ttcaggaact cgtcggagta
      421 gcggtcctcg gtcccgaacg ggatctgctc gcggacggtc gcctgctcga acgtcacccg
      481 cgtcacccgg atcgtcaagc cgttcgtgag gcgccggtcg aggcccggcg ccacctcgtc
      541 gttcttccgg aggatcacgc ccaggctgac gagcagatgg ccgacgtccg gggcgttcgt
      601 gatgacctga cgatcggcgc cgtccacctt gacacgcacc tccacggcct cgcgcagcac
      661 gatgcgatcg ccgtccgaga tcgcggcgcc acgcgacggc cggacgtagg ccttgttcgc
      721 cgagcggagg ttcacgtggg acagcacgtc ctcgacggtt cgaccggtca cgtagagggt
      781 ccgcggcttc ccattgagca cgagggtgat ctccttggcc aggaggacct ccaccctcat
      841 gtcgtcggcc accggcgcat cgaggggcgg ggagacctcg tcgtgctccc cgatctcgat
      901 gcccgtgccc tcgagaagat cgcccaccga cgaggcgaag gtgctgacgc ggcgctccgc
      961 tccgtccacc acgagcgtca ccttcttctc gagctggacg taggccaggc caccggccac
     1021 gagggccacg acgaggagca ggcgcccgaa tcccctggga cggggagcac gccgacgtac
     1081 gcgcgcggtg cgcctgctcg cccaacggtt cgcggcgcgc ggtggggaag ggagccgcgc
     1141 gctccgtgat gctcctccct caggcaaacc gcgaccgagg ctagcaaagg accccggccg
     1201 ttgccagtta cccggatgcg cgctacccgg ggagggcgaa ggccgcgcgg gcgttgcggt
     1261 cggtgatctc ctcgagctcc tcgagcccct gacctctgac ttcggccacg gcctggagtg
     1321 tcaggcgcag gttgccgggc gcgttggggc gcccccggag cggctgcggc ggcagatagg
     1381 ggctgtcggt ctccacgagg aggagctcct cgggcacgac ggcggccgcc gcccgcaggc
     1441 gctcgttctt gggataggtg atcgggccgg cgaacgagca gtggtagccg cgtctcgccg
     1501 cctccctcgc ctcctcctcg ccgccagaga agcagtggag aatcacgcgc tcgagccgct
     1561 cctcctccag gatccgcagg acgtcgggcc acgcgtcccg cgagtgcacg accacaggct
     1621 tgtcgtgctc ccgggccagc gagcagtggg cccggaagac gcgatcctgg tcctccgccg
     1681 gcgagagcat ccggtagtgg tcgaggcccg tctcgcccac ggccacgacg cggggatcgc
     1741 tcaggagctc ctcgatctcg acgccggtgg cgtcgtcgaa gtccttcgcg tcgtgcgggt
     1801 gaacccccgc ggtggcaaag accccgcgga tcgagtcggc gagctcacgg gaccggtgcg
     1861 acgactcgcg atccacgccc acgcagacga gcgtccccac gccggcctcc tcggcctcac
     1921 gcaccagctc cttcggctcc ccctccatca ggaacaggtg gcagtgggtg tcgatcacgg
     1981 ctcatccctc gaggcggggg aagagggcct cccccttcgt cacgcgggtg cctgggacga
     2041 ggccgcccca gcgggccgcc tcggggagtc gctgctcctc gagcggctcg gtgatcccga
     2101 gctgggccca cagacgcccg gcggcgctcg gcatgaccgg cgagatcagc accgcgagga
     2161 tccgcagcgt ctcggcggcc gtgtagagga tcgtcgcggt ctggtcgcgg cggtcggggt
     2221 ccttcgccag cttccagggc tcgacctcga ccaggtactc gttggcccgt cccacgatct
     2281 cccagaccgc gcccagggcc gcggtcagct ccatgtcgtt cacgaggcgg tcgtagcgag
     2341 cgaccacctc atcgatcgtg tccggcagcc ccccgtcgag cccctcggac gcgggctcgg
     2401 ggacgcctcc ctcgaacgag ctgacgatca tggccagcac gcggctggcg aggttgccca
     2461 ggccgttggc gaggtcggcg ttcgtccgcg ccaccatcga ctcccacgag aacgagccgt
     2521 cctggccgaa ggccacctcg cgcaggaagt accagcggta ggcgtccacg ccgaagtggt
     2581 ccacgagctc gaaggggtgg atgccggtga gcttcgtctt cgacatcttc tccccgccca
     2641 cgaggaggaa gccgtgggcc cagacctgct tcgggggctc gacgcccgcc gccatcagca
     2701 tcgccggcca gtagatcgcg tggaaccgga ggatgtcctt cccgatgaag tggacgtccg
     2761 ccggccacat ccgctcgaag cgctcgggat cgtcggcgaa gccggccgcc gtgatgtagt
     2821 tcgtcagcgc atcgaaccac acgtagcaga cgtggctcgg gtcccagggg aggggcacgc
     2881 cccaggcgaa cgaggtgcgc gagatcgaga agtcgcggag gccctggcgg atcgtggaga
     2941 gcacctcgtt gcgccgcgtc tcgggctcca ccgcctcggg atggcgctcg tagtgctcga
     3001 ggaggcggtc ggcgtaggcg gagagacgga agaagtagtt ctcctcggac atggcctcga
     3061 cggggcggcc gtggatcggg cagttcccgc tcacgagctc gtcctcggtg ta
//
LOCUS       DASUTW010000081         5059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_283112, whole genome shotgun sequence.
ACCESSION   DASUTW010000081 DASUTW010000000
VERSION     DASUTW010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283112"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1931)
                     /locus_tag="VF058_02700"
     CDS             complement(<1..1931)
                     /locus_tag="VF058_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207247.1"
                     /translation="MKTERAREGAPASERWGSLGGAMAWAGRVAAHPGTALAAVAAIA
                     LLMAWQFIVDASRAVPALDTAWYQWRAEYIQANDPGSLIEIEGADGSLAGGYRVAQPV
                     LAALLRIVGGVGAEVPTVVLSVFFRVAAAAALAAFAWRHRRSRLLYYLTLAVVPALFL
                     LQRFFGFLDNFFALALTGAALLLLERMRTSWVARLAVIAMLFLTGLTHPTTLALFLLA
                     LGAVAGYHFLREGFSIRAALRSDVAPMLWAGAVSVVLMFAAWLGGMWGPSAGLNEAAV
                     PPPQPVSYFVDRSINVFKGMQPVTLVPLFLIGFVALAVDAWRRRERLAEITVAWDLPL
                     LGMLGFLIGAAYPYFRFFNATLAPLLTIAVGFAVIIGAAARLRDRRLSLVASVVAAAA
                     TVGILATWWVGGISSWNDGSTWLKPDVRTSLAAISGYLEAEPEGRKAVVVVDAQPDGV
                     VPYGRYKEFANALYAGVEGGRIDDTAPFFGRIEDLEAGTATSIGDEQYDTMASDSAAE
                     ALPLLEDEAVVLMPLVFNEPSDNGAFLEGCAECRELGDSEVYAIPELSTAAVSEEAVA
                     AGAEAADQARAFAESPPGPFEGLGSSVLSLLGLLLILAVPGWLLLRGIPGRDPIEAIA
                     LVPMLSVTLVTTVGVVALG"
     gene            complement(1944..3590)
                     /locus_tag="VF058_02705"
     CDS             complement(1944..3590)
                     /locus_tag="VF058_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6207248.1"
                     /translation="MLKSRLLSIALVLAAACSGGAARTPPADPSPTGRSGGAPEARET
                     PRPPSGPFEEVACRLPEPHLRRIWAGYHPGRSGHLQIVPKPPDVVGAWLSHSGPWRFV
                     QHVPLLFYGPGHVPEGVRVSRTATLADVAPTQAALLDFDFDAPDGEPLAEAVAGTGRP
                     PKLIVTMIWDSAGMNVLEEHPDAWPTLRRLIERGTFFEETFVGSSPSVTPAVHATIGT
                     GAFPRNHGIVDLRFRVDGVPTAARGKAAANLLTPSLSDLFDVAHDNEPVVGLIGSEGT
                     LGMLGHGAFWEGGDSDVAVTRTRTEWTLEGLNAEAFAFPGYAGGFPGLEEAIRRLDLE
                     DGRADGRWLGEPMDEGVEGVSATPAFGDYQRSMVREVIEREGFGRDEIPDLLYVNFRE
                     IDAVGHRWGMHGPQMEAVVRSTDEALGDLVRTLDREVGAGEWVLTMTADHGATPDPEP
                     LEGFVIGPQEVRKDIETEFGPIVGDLRVTQVWLELEEMAERGVSLQEVATFVADYRRS
                     ENASDPASLSAEQGEERVFGTVFPSAVLDDPPCIITARSG"
     gene            complement(3622..>5059)
                     /locus_tag="VF058_02710"
     CDS             complement(3622..>5059)
                     /locus_tag="VF058_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6207249.1"
                     /translation="RIRRGYHPERSGEIQIVPRLPAVVASWLSHAGPWDHVQRVPLFL
                     YGPGHVPAVGRVDRPVTLADLAPTLARILGTDFQAPDGRPLREAVAVADPPRLILTMI
                     WDSAGRNVLDEHPEAWPTLRALIPGGAWFEEATVGTSPSTTPAVHATIGTGAFPSRHG
                     LVDLHFRWGGRMAQILDERPAFLSMPTLADRYAASTGQAAKVGLIASLGTLGLIGHGA
                     SIEGGGRHLVAMHNEGVWGIGEAYAEDFRFPAYLHDVRGLTASIRRLDLEDDAVDGRW
                     LGEPVLTPGEDLSFSPAWAEHQTRILEEVVEREGFGRDAVPDLLFTNYKQVDAVGHRW
                     GMHGPQMEAVVRSSDTALRTLMAILDREVGEGEWVLALTADHGSIPDPAGAPIIDLQE
                     LRRDLAEAFGAAVEQVRPTQVWVDERALASRGATLEDVAELLGGYRRSDNHELPPGEP
                     DTRLFAAAFPSEVLLTASCVPSQRGAFG"
BASE COUNT          760 a         1902 c         1770 g          627 t
ORIGIN      
        1 atccccagcg ccacgacgcc gaccgtcgtg accagcgtca ccgacagcat cgggacgagc
       61 gcgatcgcct cgatcgggtc ccgacccggg atcccgcgca gcagcagcca cccggggacg
      121 gccaggatga gtagcaaccc cagcagcgac agcacgctcg acccgaggcc ctcgaagggc
      181 cccggcgggc tctcggcgaa cgccctcgcc tggtcggccg cctccgcccc cgccgccacg
      241 gcctcctcgg agaccgccgc cgtcgagagc tcggggatcg cgtagacctc gctgtcgcct
      301 agctcccggc actctgcgca cccctcgagg aacgcgccgt tgtccgaagg ctcgttgaag
      361 acgagcggca tgaggaccac cgcctcgtcc tccaagaggg gcagggcctc ggccgcgctg
      421 tcggacgcca tcgtgtcgta ctgctcgtcg ccgatcgacg tggccgtccc cgcctccaag
      481 tcctcgatcc gcccgaagaa cggcgcggtg tcgtcgatgc gacccccctc cacccccgcg
      541 tagagcgcat tggcgaactc cttgtaccgg ccgtagggca cgacgccgtc gggctgcgcg
      601 tccaccacca cgacggcctt ccgcccctcg ggctcggcct cgaggtagcc ggagatggcg
      661 gccaggctcg tccgcacgtc gggcttcagc caggtgctcc cgtcgttcca cgacgagata
      721 cccccgaccc accaggtggc gaggatcccc acggtggccg ccgcggccac caccgatgcg
      781 acgagggaga ggcgacggtc gcgcagacgg gcggcggcgc cgatgatcac cgcgaagccc
      841 accgcgatcg tgagcagggg ggccagcgtg gcgttgaaga accggaagta ggggtaggcg
      901 gccccgatca ggaagcccag catcccgagc agcgggaggt cccaggccac ggtgatctcc
      961 gcgagccgct cccgcctccg ccaggcgtcc accgccaggg ccacgaaccc gatcaggaac
     1021 agcggcacga gcgtgacggg ctgcatcccc ttgaagacgt tgatcgaccg gtccacgaag
     1081 tacgagaccg gctgcggcgg gggcaccgcg gcctcgttga ggccggcgct cggaccccac
     1141 atcccgccga gccaggccgc gaacatcagc acgaccgaca cggccccggc ccacagcatc
     1201 ggggccacgt cggagcggag agccgcccgg atcgagaacc cctcccgcag gaagtggtag
     1261 ccggccaccg cgcccagcgc cagcaggaac agcgcgagcg tcgtggggtg agtgaggccg
     1321 gtcaggaaca gcatcgcgat gaccgcgagc cgcgccaccc agctcgtccg catccgctcc
     1381 aacaggagga gggcggcgcc cgtcagcgcc agcgcgaaga agttgtccag gaacccgaag
     1441 aaccgctgca gcaggaagag agccggcacg acggccagcg tgaggtagta gaggagccgg
     1501 ctgcggcggt gccgccaggc gaaggcggcc agcgccgcgg cggcggccac gcggaagaac
     1561 acggagagga cgacggtggg gacctcggcc cccaccccgc ccacgatcct gagcagggcg
     1621 gccagcaccg gctgggccac tcgatagcct ccggccagcg acccgtcggc cccctcgatc
     1681 tcgatcaggg accccgggtc gttcgcctgg atgtactcgg cccgccactg gtaccaggcc
     1741 gtgtcgagcg cggggaccgc gcgggacgcg tccacgatga actgccaggc catcagcagg
     1801 gcgatcgcgg ccaccgcggc cagcgccgtc cccgggtggg cggcaacacg gccggcccac
     1861 gccatcgctc cccccagaga cccccagcgc tccgaggcgg gggcgccctc gcgcgcccgt
     1921 tccgttttca tcgcactcct tcgtcacccg ctccgtgccg tgatgatgca cggaggatcg
     1981 tccaggactg cgctggggaa gaccgtcccg aacacgcgct cctctccctg ctccgcgctc
     2041 agcgacgcgg gatcggaggc gttctcggac ctccggtagt ctgccacaaa ggtggccacc
     2101 tcctgcaggc tgacccctcg ctcggccatc tcctcgagct ccagccagac ctgggtgacg
     2161 cgcaggtccc cgacgatggg cccgaactcc gtctcgatgt ccttccggac ctcctggggc
     2221 ccgatgacga acccttccag tggctcgggg tcgggggtcg ccccgtggtc cgcggtcatc
     2281 gtgaggaccc actcccccgc ccccacctct cgatcgaggg tccgcacgag gtcgcccagc
     2341 gcctcgtcgg tggaacggac cacggcctcc atctggggcc cgtgcatccc ccaccggtgc
     2401 cccaccgcgt cgatctcgcg gaagttgacg tagaggaggt cggggatctc gtctcggccg
     2461 aagccttcgc gctcgatcac ctcgcggacc atcgaccgct gatagtcgcc gaacgccggc
     2521 gtggccgaga ccccctccac gccctcgtcc atcggctccc ccagccatcg cccgtcggcc
     2581 cgcccgtcct cgaggtcgag cctccggatc gcctcctcga gccccgggaa gccgccggcg
     2641 taccccggga aggcgaaggc ctcggcgttc agcccctcga gggtccactc ggtccgggtg
     2701 cgggtcacgg caacgtccga gtccccgccc tcccagaatg cgccgtggcc gagcatgccc
     2761 agggtcccct ccgacccgat caggcccacc accggctcgt tgtcgtgggc gacgtcgaag
     2821 aggtcggaga gggagggggt caagaggttc gcagcggcct tgcctcgggc ggccgtgggc
     2881 acgccgtcca cgcggaagcg caggtccacg atcccgtggt tccgggggaa ggcccccgtg
     2941 ccgatcgtgg cgtggacggc cggcgtcacc gagggtgacg acccgacgaa cgtctcctcg
     3001 aagaacgttc cccgctcgat caggcggcgc agggtgggcc aggcgtcggg atgctcctcc
     3061 agcacgttca tgccggccga gtcccagatc atcgtcacga tgagcttcgg aggacgcccg
     3121 gtgccggcca ccgcctcggc gagcggctct ccgtccggcg catcgaagtc gaagtcgagg
     3181 agcgccgcct gcgtcggcgc cacgtcggcg agcgtcgccg tccggctcac ccgaaccccc
     3241 tcggggacgt gccccggccc gtagaagagg agcggcacgt gctggacgaa cctccaggga
     3301 ccggagtggg acagccacgc cccgacgacg tcggggggct tcgggacgat ctggaggtgg
     3361 ccggagcgcc cgggatggta ccccgcccag atccggcgga ggtgcggctc cggcagtcgg
     3421 caggcgacct cctcgaaggg cccgctggga ggacgcggtg tctcccgcgc ctcgggcgcg
     3481 cctcctgatc gtcccgtcgg cgacgggtcc gcaggcggcg tcctggccgc gcctccggag
     3541 caggcagcgg ccaggacgag cgcgatgctc agcagtcgag atttcagcac ggatgatcct
     3601 gggaccgttc ggcgcgacag tctacccgaa ggcccctcgt tggctgggga cgcaggacgc
     3661 ggtcaggagg acttccgagg ggaaggccgc ggcgaacagc cgcgtgtcgg gctctcccgg
     3721 gggcagctcg tggttgtcgg agcgccgata gcctccgagc agctccgcca cgtcctccag
     3781 cgtggcccct cgggaggcga gcgcacgctc gtcgacccag acctgggtgg gtcgcacctg
     3841 ctccaccgcg gctccgaagg cctccgccag gtcgcgccgc agctcctgga ggtcgatgat
     3901 cggcgccccg gccgggtccg ggatcgagcc gtggtcggcg gtgagcgcca gcacccactc
     3961 gccctccccc acctcgcggt cgaggatggc catgagggtc ctgagcgcgg tgtcggagga
     4021 gcggaccacg gcctccatct ggggtccgtg catcccccac cggtgcccca ccgcgtcgac
     4081 ctgcttgtag ttcgtgaaga ggaggtccgg caccgcgtcc cggccgaagc cctcgcgctc
     4141 gacgacctcc tccaggatcc gggtctggtg ctcggcccag gcgggcgaga aggagaggtc
     4201 ctcgcccggc gtcaggacgg gctcccccag ccagcggccg tcgacggcgt cgtcctcgag
     4261 atcgaggcgc cggatcgacg ccgtgaggcc gcgcacgtcg tgcaggtacg cggggaagcg
     4321 gaagtcctcc gcgtacgcct ccccgatccc ccacacgccc tcgttgtgca tggccacgag
     4381 gtgccggccc ccgccctcga tcgaggcccc gtggccgatg agccccagcg tccccagcga
     4441 ggcgatcagc cccaccttcg ccgcctgtcc cgtgctcgcc gcgtaccggt cggcgagcgt
     4501 aggcatcgag aggaaggcgg gacgctcgtc gaggatctgg gccatcctgc cgccccagcg
     4561 gaagtggagg tccacgagcc cgtgccggga cgggaaagcg ccggtcccga tcgtggcgtg
     4621 cacggccggc gtggtcgagg gcgacgtccc gaccgtcgcc tcctcgaacc acgccccgcc
     4681 cgggatcagg gcgcgcagcg tgggccaggc ctcggggtgc tcgtcgagga cgttgcgtcc
     4741 cgccgagtcc cagatcatcg tgaggatcag gcgaggcgga tccgcgacgg ccaccgcctc
     4801 gcgcaggggc cggccgtcgg gagcctggaa gtcggtgccg aggatccgcg ccagcgtcgg
     4861 ggcgagatcc gccagcgtca ccggacggtc cacgcgtccg accgccggca cgtgcccggg
     4921 gccgtagagg aagagcggca cgcgctggac gtgatcccag ggccccgcgt gcgagagcca
     4981 cgacgcgacg accgccggga gccgagggac gatctggatc tcgcccgagc gctcgggatg
     5041 gtagccgcgt cggatgcgc
//
LOCUS       DASUTW010000082         4376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1189658, whole genome shotgun sequence.
ACCESSION   DASUTW010000082 DASUTW010000000
VERSION     DASUTW010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189658"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            384..557
                     /locus_tag="VF058_02715"
     CDS             384..557
                     /locus_tag="VF058_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207250.1"
                     /translation="MALFGARGWIVHPEEGVRRVGHKHVATTQAIYQHAVPALEEEAI
                     SRFAELLERAKSA"
     gene            complement(657..730)
                     /locus_tag="VF058_02720"
     tRNA            complement(657..730)
                     /locus_tag="VF058_02720"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(694..696),aa:Met,seq:cat)
     gene            complement(762..2282)
                     /locus_tag="VF058_02725"
     CDS             complement(762..2282)
                     /locus_tag="VF058_02725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6207251.1"
                     /translation="MSLRSRVLIGTLCLVAAALLLTDFAVYRFLSSFLHGRVDDQLES
                     GMWAAPRILELTGDRSGPSPPVLERGRPETVYPSGTYFAHLDETGRLIRDFMITLESR
                     REGSRPDPDPAAPALPEGLPGTQDTESGSPSSRFLAESEDGTPYRVLADALTGGGTIL
                     VAIPLTGTEATLQRLLLIEAVVTAGVLLAVGVLGLFVVRRGLRPLDRIADSAGAIAAG
                     DLSSRLAPANDRTEVGRLGLALNTMLAQIETAFREREESEERLRRFVADASHELRTPL
                     TSIQGYAELFRRGAAERPEDLAKAMRQIESESRRVSELVDELLLLARLDQRDTVESPI
                     RLTRVDLTTIVAEAVEAARDLDPDRRIGLVASGPVIVMGDDVRLRQVLDNLLSNARRY
                     TPTGTHVRVHVGVEEGLAVAEVADDGPGIDAEHAERIFERFYRVDRSRSRQNGGVGLG
                     LSIVAAVAEAHGGRATCEPVMPHGVRFRIELPLAERHEDGVVEPPPDEARSAPSRR"
     gene            complement(2287..3018)
                     /locus_tag="VF058_02730"
     CDS             complement(2287..3018)
                     /locus_tag="VF058_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007072772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6207252.1"
                     /translation="MRERSAVAKRHAEGPVPGLARILVIDDDPGITDLVATALRYEGF
                     SVQVAATGREGLQATTSFRPHLLVLDVMLPDGDGFEIQQRLAAEGMDVPILFLTARDT
                     TEEKVRGLLEGADDYVTKPFSLEELVARIHSVLRRNADQVTRNLVVADLEMDEDTHEV
                     WRGGERIELSPTEFNLLRFLMLNPRRVVSKSQILSHVWHYDFQGDPSVVETYVSYLRK
                     KIDRREPRLLHTVRGVGYTLRPPGA"
     gene            3150..3452
                     /locus_tag="VF058_02735"
     CDS             3150..3452
                     /locus_tag="VF058_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207253.1"
                     /translation="MRILLLGALLVASACASSPAGGEAPVPRELTGAITEIARGDGGE
                     IEVFTVEGTHRIRIDQGRDYGFNLEHLAEHQTTGDPVRVTTESRYGVAYAVEILDA"
     gene            complement(3449..>4376)
                     /locus_tag="VF058_02740"
     CDS             complement(3449..>4376)
                     /locus_tag="VF058_02740"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX6207254.1"
                     /translation="PLINAWGEIFPDLLTPLEEASPELREHFRYPEDIFLVQARQFSS
                     YHVTNPGQFYFGEDFWSLPIVAQGDPGIEGEQTNEPVQLEPYYVLLPLPGEEAQGERF
                     MLFIPFTPADRPNMVAWMAAVSDPDQYGRMVSFEFPLERAVPGPQNVAAFISQETEIA
                     REISLLDQLGSNVIYGDLLAIPVGRSFLYVQPLYLESAQAGSAIPELQRVVVVNGDTV
                     VMEPTLEEALARAFALEPEEPEQPPEPGEEPEEPEQPVPGDVARLLAEAQRHFELAQA
                     ALERGDLGEYQRQIGLAEEAIAAAVEAAGAAP"
BASE COUNT          673 a         1552 c         1497 g          654 t
ORIGIN      
        1 gctatgtcga agactggtgt tgaatccccg gcgggggcaa acaggtgggt cctggcgtcg
       61 acagtcccgc tctctcgggc agaagtatcc cgacccaatc gaaggggccc cgcgtgtgca
      121 gctcccgcgg ctccgttgac gatctccggc caaggtcggg cgcgcgaagg atgccagagg
      181 acgccgcgtc ggcgcctaag aatgtaggca atccgctcct cgtccgtcct cgccattcct
      241 ttcagaggtt ctccaccgag tcgacaactc tggctgcgat ggcggccagc gctgcattct
      301 caaagagacg gcggtgggcc ttgggcttag aagtccacag cggtagatag gtacgcgggc
      361 catcaggtca gccgcgtaag gccatggctc tcttcggagc ccggggatgg atcgttcatc
      421 cggaggaggg tgtccgccgc gtggggcaca agcacgtcgc caccacccag gcgatctacc
      481 agcacgcggt gccggcgctc gaggaggaag ccatctcgcg cttcgccgag ctgctggaga
      541 gggcgaagag cgcctagctc ccggtatcag acgggtatca gagagcccca aaacaacgct
      601 cacacgaaag gggcgccgat cgctcggcac cccttctgac ctgctctttc gcctcgttgc
      661 gcgggctgga tttgaaccag cgacctctgg gttatgagcc cagcgagcta ccagactgct
      721 ccaccgcgca ccccgatgct accaagggcc gctgaaggcg tctaccggcg cgaaggcgcg
      781 gagcgcgcct cgtcgggtgg cggctccacg acgccgtcct cgtggcgttc ggccagcggc
      841 agctcgatcc ggaaccgcac cccgtgcggc atcaccggct cgcacgtggc tcgccccccg
      901 tgcgcctcag cgacggcggc cacgatcgag agcccgagcc ccaccccgcc gttctgcctc
      961 gacctcgagc ggtccacccg gtagaagcgc tcgaagatcc gctccgcatg ctccgcgtcg
     1021 atcccgggcc cgtcgtcggc cacctccgcg acggcgagac cctcctcgac cccgacgtgg
     1081 acccgaacgt gcgtgcccgt cggcgtgtac cgccgcgcgt tcgacagcag gttgtcgagg
     1141 acctggcgca gccggacgtc gtcgcccatc acgatcacgg gtccgctggc aacgagaccg
     1201 atgcgtcgat cggggtcgag gtctcgggcc gcctccaccg cctctgccac gatcgtcgtg
     1261 aggtcgacgc gcgtcagccg gatcgggctc tccaccgtgt cccgctggtc gagccgagcg
     1321 agcagcagca gctcgtccac gagctcgctc acgcggcggc tctcgctctc gatctgtcgc
     1381 atcgccttgg ccaggtcctc cggacgctcc gcggcccccc gccggaagag ctccgcgtag
     1441 ccctggatgg aggtgagcgg ggtcctcagc tcgtgcgacg cgtccgccac gaatcgacgc
     1501 aggcgctcct cggactcctc ccgctctcgg aacgcggtct cgatctgggc gagcatggtg
     1561 ttgagggcca ggcccaggcg ccccacctcg gtgcgatcgt tcgccggcgc cagccgcgat
     1621 gagagatcgc cggccgcgat cgcccccgcg gaatccgcga tccggtcgag cggccgcagc
     1681 ccccgccgca cgacgaaaag cccgagcacc cccaccgcca agaggacccc cgcggtgacc
     1741 acggcctcga tcaggaggag ccgctgcagg gtcgcctcgg tgccggtcag cgggatcgcc
     1801 accaggatcg tcccccctcc cgtgagggca tcggcgagca cacggtaggg cgtgccgtcc
     1861 tccgactcgg cgaggaaccg gctggagggc gacccgctct ccgtatcctg cgtgccgggc
     1921 agcccctcgg ggagggcggg agcggccggg tccgggtcgg gacgagaccc ttctcgccgc
     1981 gactccagcg tgatcatgaa gtcccggatc agcctgccgg tctcgtcgag gtgagcgaag
     2041 tacgtgcccg acgggtacac ggtctcaggc ctcccccgct cgagcacggg aggcgatgga
     2101 ccggatcggt ctcccgtgag ctcgaggatg cgaggcgccg cccacatccc gctctcgagc
     2161 tgatcgtcca cccggccgtg gaggaacgac gagaggaacc gatacacggc gaagtcggtc
     2221 agcagcagcg ccgcggccac gaggcagagc gtcccgatca gcacacgact gcggagggac
     2281 acgcgatcac gctcccgggg ggcgcagcgt gtacccgacg ccccgcacgg tgtgcaggag
     2341 cctcggctcc cgccggtcga tcttcttccg caggtagctc acgtacgtct ccacgacgct
     2401 ggggtcgccc tggaagtcgt aatgccagac gtggctcagg atctgggact tcgacaccac
     2461 gcggcgaggg ttgagcatca ggaaccggag gaggttgaac tccgtgggcg agagctcgat
     2521 ccgctcgcct cctcgccaga cctcgtgcgt gtcctcgtcc atctcgaggt cggccacgac
     2581 caggttgcgg gtgacctggt cggcgttgcg gcggagcacg gagtggatcc tcgccaccag
     2641 ctcctccagg ctgaacggct tggtcacgta gtcgtcggct ccctcgagca ggccccgcac
     2701 cttctcctcg gtcgtgtccc gggcggtcag gaagaggatc ggcacgtcca tcccctcggc
     2761 cgccaggcgc tgctggatct cgaacccgtc gccgtcgggg agcatcacgt cgagaaccag
     2821 gagatgaggg cggaacgagg tcgtggcctg gaggccctcg cgacccgtgg cggccacctg
     2881 cacgctgaac ccctcgtagc ggagggcggt agcgaccaga tcggtgatcc cggggtcgtc
     2941 gtcgatcacg aggatccggg cgagccccgg cacgggtccc tcggcgtgac gtttcgcgac
     3001 ggcagaccgc tccctcatcg ccacgatcct ctcggctgca ggctcctcag gcggggccgc
     3061 gaagtatatc gagctggagc gctcgccgca tgctcggacg gccgaggccg tgtcgctccc
     3121 gaccgagtcc tccacgctac agtgctcgca tgcgcatcct cctgctcggg gccctcctcg
     3181 tggcctccgc gtgcgcctcg tcccccgccg ggggggaggc gcccgtccct cgggagctga
     3241 ccggcgcgat caccgagatc gcacgggggg acgggggcga gatcgaggtc ttcaccgtcg
     3301 aggggacgca ccggatccgc atcgatcagg gtcgggacta cgggttcaac ctggagcacc
     3361 tcgcggagca tcagaccacg ggggaccctg tgagggtcac gaccgaatcg cgatacggcg
     3421 tcgcgtacgc cgtggagatc ctcgacgcct agggcgcggc gccggcggcc tcgaccgccg
     3481 cggcgatcgc ctcctccgcc agcccgatct gccgctggta ctcgccgagg tccccgcgct
     3541 ccagcgccgc ctgggccagc tcgaagtgac gctgggcctc ggccagcagc cgggccacgt
     3601 cccccggcac gggctgctcc ggctcctccg gctcctcgcc cggctccggt ggctgctccg
     3661 gctcctccgg ctcgagcgcg aaggccctgg ccagcgcctc ctccagcgtg ggctccatca
     3721 ccaccgtgtc gccgttgacc accaccactc gctgaagctc ggggatggcg gagcccgcct
     3781 gcgcggactc gaggtagagg ggctggacgt agaggaacga tcgacccacg gggatggcca
     3841 ggaggtcgcc gtagatcacg ttggacccga gctggtcgag gaggctgatc tcgcgggcga
     3901 tctcggtctc ctgggagatg aacgcggcga cgttctgagg tcctgggacg gcccgttcca
     3961 gggggaactc gaaggacacc atgcggccgt actgatccgg gtcggagacc gcggccatcc
     4021 aggccaccat gttcgggcga tcggcgggcg tgaacgggat gaagagcatg aaccgctccc
     4081 cctgagcctc ctcgccgggg agcggcagga ggacgtagta gggctcgagc tggaccggct
     4141 cgttcgtctg ctcgccttcg atgcccgggt cgccctgcgc cacgatcggc agagaccaga
     4201 agtcctctcc gaagtagaac tgcccggggt tggtcacgtg gtagctggag aactgcctgg
     4261 cctgcaccag gaagatgtcc tccgggtagc ggaagtgctc ccggagctcg ggtgaggctt
     4321 cctccagcgg cgtgaggagg tcggggaaga tctcccccca cgcattgatc agcggg
//
LOCUS       DASUTW010000083         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_671940, whole genome shotgun sequence.
ACCESSION   DASUTW010000083 DASUTW010000000
VERSION     DASUTW010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671940"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="VF058_02745"
     CDS             <1..1206
                     /locus_tag="VF058_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX6207255.1"
                     /translation="LTAAQPENNVHRILLEMLSVTLSKDARARAVQLPAWNEALGLPR
                     AWDQQWSLRAQQILAEETDLLEHADLFEGSKVVAERTDEIATAAWEELGRVLDMGGSV
                     EALGYMKERLVAKHAERLRAIEEGELTLVGVNRYTETEVSPLVEGLGEGFASFHEVNP
                     ELEAEQAESVERWRAERDGAAAARALDGLRAAAEGGENLVPVSIEAARAGVTTGEWGA
                     ALREVFGEFRAPTGVSGASGVGADAGSLREARARVEEAARRAGVARLRMLVAKPGLDG
                     HSNAAEQVAVRARDAGFEVVYQGIRLTPEQIARAAAEEDVHVVGLSVLSGAHNLLVPK
                     VLERLREEGVDPGRVPVVVGGIIPDDDAAKLSELGVARVYTPRDYDLTRQVAEIADLI
                     AASGDAGGR"
     gene            1203..1985
                     /locus_tag="VF058_02750"
     CDS             1203..1985
                     /locus_tag="VF058_02750"
                     /inference="COORDINATES: protein motif:HMM:NF004869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HEX6207256.1"
                     /translation="MSDERLPEKPWRTAIGRVSPNEILVRGYDLIELIGTRSFGDVTY
                     LLLSGELPEGNEGRMVEAMLVSAAEHSIVAPSVAAARYIASTGVPLQAAVSVGVAGMG
                     DFHGGAVEAGAAMLREAGELSADPSEAADGIVRAIRERGERIPGYGHVVHDPDPRARR
                     LFEVAEDLGLRGRWCAIAQEIEPATERHLGRRLAMNVDGAMAALLLELGLDPGLGKAF
                     YVIGRTPGLVAHVHEERTRERPYRDPGWENVEYDGPPPRDLP"
     gene            2038..>2178
                     /locus_tag="VF058_02755"
     CDS             2038..>2178
                     /locus_tag="VF058_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX6207257.1"
                     /translation="MKPEDVKKVAVIGTGIMGSGIVEVCAKADCEVVTVEVDDQAMDR
                     GRQ"
BASE COUNT          296 a          721 c          896 g          265 t
ORIGIN      
        1 ctgacggcgg cccagcccga gaacaacgtc caccggatcc tgctcgagat gctctcggtg
       61 acgctgtcca aggacgcccg ggcgcgggcc gtccagctcc cggcgtggaa cgaggccctc
      121 ggcctgccga gggcgtggga ccagcagtgg tcgctccggg cccagcagat cctggccgag
      181 gagaccgacc tgctcgagca cgccgacctc ttcgagggat cgaaggtggt ggccgagcgg
      241 accgacgaga tcgccacggc cgcgtgggag gagctgggcc gcgtgctgga catgggcggg
      301 tcggtcgagg cgctcggcta tatgaaggag cggctcgtgg cgaagcacgc cgagcgcctt
      361 cgggccatcg aggaggggga gctgactctg gtgggcgtga accgctacac cgagacggag
      421 gtctcgccgc tcgtggaggg gctgggcgag gggttcgcct cgttccacga ggtgaacccc
      481 gagctggagg cggagcaggc cgagagcgtg gagcgctggc gggccgagcg ggacggggcg
      541 gcggccgcgc gggcgctcga cgggctccgc gcggccgccg agggcggcga gaacctcgtg
      601 ccggtctcga tcgaggccgc ccgggccggg gtgacgacgg gggagtgggg ggccgccctg
      661 cgcgaggtgt tcggcgagtt ccgggccccc accggggtga gcggggcctc cggggtagga
      721 gccgacgcgg ggtccctgcg ggaggcgcgg gcccgcgtgg aggaggcggc ccgtcgagcc
      781 ggcgtcgcgc gcctccggat gctggtggcc aagccggggc tggacggcca ctccaacgcg
      841 gcggaacagg tggccgtccg ggcgcgcgac gccggcttcg aggtggtcta ccaggggatc
      901 cggctcaccc ccgagcagat cgcccgggcc gcggccgagg aggacgtgca cgtcgtcggg
      961 ctgtcggtgc tcagcggcgc gcacaacctg ctcgtgccaa aggtgctgga gcgtctccgc
     1021 gaggagggcg tggacccggg gcgggtgccg gtggtcgtgg gcgggatcat ccccgacgac
     1081 gacgcggcga agctctccga gctcggcgtg gcgcgcgtct acacgccccg ggactacgac
     1141 ctgacgcgtc aggtggcgga gatcgccgac ctcatcgccg cgagcggcga cgcaggcgga
     1201 cgatgagcga cgagcgcctc ccggagaagc cctggcgcac ggcgatcgga cgggtttcgc
     1261 cgaacgagat cctcgtgcgg ggctacgacc tgatcgagct gatcgggacg cgctcgttcg
     1321 gcgacgtcac ctacctgctg ctttccggcg agctccccga ggggaacgag ggccggatgg
     1381 tggaggccat gctcgtctcg gcggccgagc actcgatcgt cgcgccctcg gtggcggccg
     1441 cccggtacat cgcctcgacc ggcgtcccgc tccaggccgc ggtctccgtg ggcgtggccg
     1501 ggatggggga cttccacggg ggagcggtcg aggcgggggc ggcgatgctg cgggaggcgg
     1561 gggagctgag cgccgatccc tcggaggccg ccgacgggat cgtccgggcc atccgcgagc
     1621 ggggcgagcg catccccggc tacgggcacg tggtccacga ccccgatccc cgcgcccgtc
     1681 ggctgttcga ggtggccgag gacctcggcc tccggggccg ctggtgcgcg atcgcccagg
     1741 agatcgagcc ggccaccgag cggcacctcg gccgccgcct ggcgatgaac gtggacggag
     1801 cgatggccgc gctcctgctc gagctgggcc tggaccccgg cctggggaag gccttctacg
     1861 tgatcggccg cacgcccggg ctcgtggccc acgttcacga ggagcggacg cgggagcgcc
     1921 cctaccgcga cccgggctgg gagaacgtcg agtacgacgg cccgcccccc cgggacctgc
     1981 cctgatggca cgccccgcgg cgcggggcta ggctgtggcc tgacccaagg aggacggatg
     2041 aagcctgagg acgtcaagaa ggtggcggtg atcggcaccg ggatcatggg gtcgggcatc
     2101 gtcgaggtct gcgccaaggc cgactgcgag gtcgtgacgg tggaggtcga cgaccaggcg
     2161 atggaccggg gccgtcag
//
LOCUS       DASUTW010000084         3030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_542348, whole genome shotgun sequence.
ACCESSION   DASUTW010000084 DASUTW010000000
VERSION     DASUTW010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542348"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..558
                     /locus_tag="VF058_02760"
     CDS             61..558
                     /locus_tag="VF058_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX6207258.1"
                     /translation="MADKSSFDVVSEVDAAEVTNAVDQARREVATRFDFKGTDTSIEQ
                     DEDLVEVRSSTEERLKAAVEVFKDKCVKRKISLKALSEGPVLPGAKATYKQSVHINRG
                     ISQDKAREIVKFIKDLKLRKVQTAIQGEQLRVSAPKKDDLQAVIGALREADFGIPLQF
                     RNYRG"
     gene            complement(555..1796)
                     /locus_tag="VF058_02765"
     CDS             complement(555..1796)
                     /locus_tag="VF058_02765"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX6207259.1"
                     /translation="MTTRRLAILTGLLVSVQALIPLSFAGAQVDPCSPVVIPGLCNPE
                     PSPKPEPNPKPKPKPDDDRRDDRRGDGIGRGAGGSGGGGGTRVIPRRGGGQPGSPGFV
                     LGGPENTADLIETLTRLTRFGVPLRRSVASVVGPFPVAGLAWWADDWHACRDGCTRLH
                     EGLDIFAESGTPLVAAADGVVTQKGEGTLSGLHVEIEDAEGTQYFYAHLSGWADGLLI
                     GQRVRVGQVLGYVGNTGNAVNTPPHVHFEYQPNGVPQPPKPVVDRWLRIAEERAADLV
                     RQYAGEEPIELASLDTFRLTRLFDLAGGGLDLSPGSARLLVLAGLQPAASSLEVTRRT
                     IDEMAWEIDWGEHVRARLDELIGEYEEYAGLDGLGGPPFLTVLNGERLDEASLDEQDL
                     DEQDLDEHDLDERVQPAEVGD"
     gene            complement(1858..2706)
                     /locus_tag="VF058_02770"
     CDS             complement(1858..2706)
                     /locus_tag="VF058_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX6207260.1"
                     /translation="MLGINHVKSWLLIGGLAGLFVALGAMLGGGRGATIALVLAVVFN
                     GIMYWSSDKIAIAATRSKPVTRDQAPELYAIVEELAMGAKAPMPRIYVSEMAQPNAFA
                     TGRSPEHAAVSVTRGILQILDRRELRGVLAHELSHVLNRDILVSSIAGAIASAFTYLA
                     YLVFFLGGDDDSPLSGIGFLLLWLVGPLAAGLIQMAVSRSREYQADESGAMLSQDPEA
                     LAGALQKLEQASRVVPARVNPAHAHVFIVNPLRGRRGGGIASLFSTHPPTEERVRRLE
                     ELGSRI"
     gene            complement(2760..>3030)
                     /locus_tag="VF058_02775"
     CDS             complement(2760..>3030)
                     /locus_tag="VF058_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207261.1"
                     /translation="FWAKFQIFAVAIVADQAYLAVAVVVNSVISLYYYFSIVRQMFFV
                     EGLEERPFRASALVTGVVALAAVAIVVVGIFPDLFAGFPDRLTVP"
BASE COUNT          485 a         1094 c         1042 g          409 t
ORIGIN      
        1 cgacgaccgt cgccggcggc gcccaccccg cacggcctcc tctgtcgtag actcgcggcc
       61 atggccgaca agagctcgtt cgacgtcgtc tccgaggtgg acgcggccga ggtcaccaac
      121 gccgtggacc aggcccgccg cgaggtggcg acgcgcttcg acttcaaggg gaccgacacc
      181 tcgatcgagc aggacgagga cctggtcgag gtccgcagct ccaccgagga gcgcctcaag
      241 gccgcggtgg aggtcttcaa ggacaagtgc gtgaagcgga agatctcgct gaaggcgctg
      301 tccgaggggc ccgtgctccc cggggcgaag gcgacgtaca agcagagcgt ccacatcaac
      361 cggggcatct ctcaggacaa ggcccgcgag atcgtcaagt tcatcaagga cctgaagctg
      421 aggaaggtcc agaccgcgat ccagggggag cagctccggg tctcggcccc caagaaggac
      481 gacctccaag cggtgatcgg cgccctgcgg gaggccgact tcgggatccc gctgcagttc
      541 cggaactacc gaggctagtc ccccacctcc gcaggctgga cccgctcgtc gaggtcgtgc
      601 tcgtcgaggt cctgctcgtc gaggtcctgc tcgtccaggc tcgcctcgtc cagacgctcc
      661 ccgttcagga ccgtgaggaa gggcggcccc cccaggccgt ccaggccggc gtactcctcg
      721 tactcgccga tcagctcgtc gagccgagct cggacatgct cgccccagtc gatctcccag
      781 gccatctcgt cgatcgtccg ccgcgtgacc tcgagcgacg aggccgccgg ctgcagcccg
      841 gccaggacga ggagcctcgc gctccccggg ctcaggtcga gcccgccccc ggccaggtcg
      901 aagagccggg tgaggcggaa cgtgtcgagg ctcgccagct cgatcggctc ctcccccgcg
      961 tactggcgga cgaggtcggc ggcccgctcc tccgcgatcc ggagccagcg gtccaccacg
     1021 ggcttcgggg gctgcggcac cccgttcggc tgatactcga agtggacgtg ggggggcgtg
     1081 ttcacggcgt tccccgtgtt ccccacgtag ccgaggacct gtcccacacg gacccgctgg
     1141 ccgatgagca ggccgtcggc ccagccggag aggtgggcgt agaagtactg ggtcccctcc
     1201 gcgtcctcga tctcgacgtg gaggcccgag agcgtcccct cgcccttctg cgtgacgacc
     1261 ccgtccgcgg cggcgacgag cggcgtcccg gactccgcga agatgtcgag gccctcgtgg
     1321 agccgcgtgc agccgtcccg gcaggcgtgc cagtcgtcgg cccaccaggc aagccccgcc
     1381 acggggaagg gccccacgac cgaagcgacc gacctccgca gcgggacccc gaacctcgtg
     1441 agtcgggtga gggtctcgat gagatcggcg gtgttctcgg ggccgccgag gacgaacccg
     1501 gggcttcccg gctgcccgcc gccgcgacgc gggatgacgc gggtgccccc tccgccaccg
     1561 cttccgccgg cgccgcggcc gatcccgtcg ccccgccgat cgtctcgccg atcgtcgtcg
     1621 ggcttcggct tgggcttcgg attgggctcc ggcttcggcg agggctcggg gttgcagagg
     1681 ccggggatca cgacgggcga gcacggatcg acctgggcgc cggcgaacga caaggggatg
     1741 agggcctgca cggacaccag cagacccgtg aggatggcga gcctcctcgt cgtcacgctc
     1801 gtgctcctcc tcccctctcc cccgcatcct gcccgacggg gcgcgatagg tacaacatca
     1861 gatccgcgag ccgagctcct cgagacgccg gacgcgctcc tcggtgggcg ggtgggtcga
     1921 gaacagggag gcgatgcctc caccgcgccg cccccgcagc gggttcacga tgaacacatg
     1981 ggcgtgcgcc gggttcaccc gggcggggac gacgcgggat gcctgctcca gcttctggag
     2041 ggcgccggcc agggcctcgg ggtcctgcga cagcatcgcc cccgactcgt cggcctggta
     2101 ctcgcgcgac ctcgagaccg ccatctggat caggcccgcc gcaagcggcc ccacgagcca
     2161 cagcaggagg aagccgatcc cggaaagagg gctgtcgtcg tccccgccga ggaagaacac
     2221 gaggtacgca aggtaggtga acgccgaggc gatcgccccg gcgatggacg agacgaggat
     2281 gtcccgattc aggacgtgcg agagctcgtg ggccagcacc ccgcgcagct cccggcgatc
     2341 gaggatctgg aggatgccac gggtcacgct cacggccgcg tgctcggggc tccgaccggt
     2401 cgcgaacgcg ttgggctgcg ccatctcgct cacgtagatg cggggcatcg gggccttcgc
     2461 ccccatcgcc agctcctcga cgatggcgta gagctccggc gcctgatcgc gcgtcaccgg
     2521 cttcgacctg gtggcggcga tcgcgatctt atcgctcgac cagtacatga tcccgttgaa
     2581 gacgacggcc agcaccagcg cgatcgtcgc cccgcggcct cccccgagca tcgcccccag
     2641 ggcgacgaac agcccggcga ggcctccgat caggagccag ctcttgacgt ggttgatccc
     2701 cagcatctcc gaacctcctg gcacgattgt agggactccc ccggcctgcg cggggcccgt
     2761 tacgggacgg tgagccggtc ggggaagccg gcgaagagat cggggaagat ccccacgacc
     2821 acgatcgcca cggcggccag ggccaccacg cccgtcacca gggcggaggc ccggaagggc
     2881 cgctcctcga ggccctccac gaagaacatc tgcctgacga tcgagaagta gtagtacagg
     2941 ctgatcaccg agttcacgac cacggccacg gccaggtagg cctggtccgc cacgatcgcc
     3001 accgcgaaga tctggaactt ggcccagaac
//
LOCUS       DASUTW010000085         2855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1060699, whole genome shotgun sequence.
ACCESSION   DASUTW010000085 DASUTW010000000
VERSION     DASUTW010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060699"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VF058_02780"
     CDS             complement(<1..324)
                     /locus_tag="VF058_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX6207262.1"
                     /translation="MEFREVVRRRFMCRSFEDREVPEEKVEAILDAARRFPSAGHTQP
                     QEFILVRDPGVKDALGRAALGQMFLARAPIVIAVVSDVDRSAAVYGERGVRFYSILDG
                     GFASML"
     gene            463..1290
                     /locus_tag="VF058_02785"
     CDS             463..1290
                     /locus_tag="VF058_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6207263.1"
                     /translation="MRRSMPYAANPIDGVRTFFEDAGGRGTPVLVYPGFSDPLEYAKS
                     SPLAKALSDDFRLIFADHRGQGRSDKPHEVSAYALPTRVADATAILDALGIDRAHYLG
                     FSWGARLGFAIGEHAPQRVLSLVLCGNQPYEWPVDGPMMRAVTEAVAAGREHGMVAFV
                     ETWESSIGERFPEPARSWMLENDPLALDAAFRSVSVEGSISRDLGKWSTPCLVYAGAD
                     DEMHQNAARAAAEIPTASFLSLPGETHFSAERVAGELLPRVLELFRSASADDVRRSP"
     gene            1404..>2855
                     /locus_tag="VF058_02790"
     CDS             1404..>2855
                     /locus_tag="VF058_02790"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX6207264.1"
                     /translation="MLSRTATWPRPLVFMLLTAAGLFAAAAPAWAEEPFRLEQQVEDR
                     VGALEGREGEVRDALERLQARDGIQLWVTYVDTFSGLPAQEWTDETATLSSLGLNDVL
                     LAVAIEDRAYAYSVDQQFPLSDAELAEVATSSIEPSLSDDDWVGAAVAAAEGISRVMN
                     EQPAADPGGETERPEGTSSPVWTLLALLLIGGIAYLAFVWGKARLRPRERPRAAAQDP
                     YERLSIDELRQRAAAQLVETDDAVKTSEQELAFAIAEFGEEQTEPFSAALEAAREDMA
                     VAFRLRQQLDDAEPEDETTERSMLVDICRRTDAASDRLDSEAEKFDELRDLRKNVPEV
                     LARLEGDAEALARRIPQAAAVLTELADTYAPEAMAAVAENVQQVEERLELAREEIASG
                     RGHLESGAPGLAAVAARAAEEATGQATRLLDAVDRLKQDLSESGRKIEAAVADTETGL
                     AEARRLKAEQLGDEIALAEAALAEARSAAAPEGG"
BASE COUNT          452 a          972 c         1037 g          394 t
ORIGIN      
        1 gagcatcgag gcgaaacccc cgtcgaggat gctgtagaag cgcaccccgc gctccccgta
       61 gacggcggcc gagcgatcga cgtccgacac gaccgcgatc acgatgggcg cccgggccag
      121 gaacatctgg cccagcgcgg cccggcccag ggcgtccttg acgcctgggt cgcggacgag
      181 gatgaactcc tggggttgag tgtgaccggc cgacgggaac cgccgggccg cgtcgaggat
      241 cgcctcgacc ttctcctccg ggacctcgcg gtcctcgaac gatcgacaca tgaaccgcct
      301 gcgaacgacc tcgcggaact ccatcagcgc tcccggtggt cgttgggctt cggaccaccc
      361 gaccctatcg cgcctgcgag gttcggaggg ggcgccgagg aacgggagcg cccttgagac
      421 gcgacctgga cggccggcga ctagtaggct cagcgaccgg gagtgaggcg atcgatgccc
      481 tacgccgcca accccataga cggcgttcgc acgttcttcg aggatgccgg aggtaggggg
      541 acgccggtgc tcgtctaccc ggggttctcg gatcccctcg agtacgcgaa gtcctcccct
      601 ctcgcaaagg cactcagcga cgacttccgt ctcatcttcg ccgaccaccg agggcagggg
      661 cgcagcgaca agcctcacga ggtctcggcc tacgcgctcc ctacacgcgt agccgatgcc
      721 accgcgatcc tcgacgcgct cggcatcgac agggcccact accttgggtt ctcgtggggc
      781 gcccggctcg gcttcgcgat cggagagcat gcgccacagc gcgtgctctc cctagtcctc
      841 tgcggcaacc agccctacga atggcccgtc gacgggccga tgatgcgtgc cgtcaccgaa
      901 gcggtggctg cagggagaga acacggcatg gtcgccttcg tcgagacgtg ggagtcctcg
      961 atcggggagc ggttcccgga accggcgcga tcctggatgc tggagaacga ccccctggcg
     1021 ttggacgccg cgttccgctc cgtctccgtc gaaggatcga tatctcgaga cctggggaag
     1081 tggagcaccc cctgcctcgt ctacgcagga gctgacgacg agatgcacca gaacgccgcg
     1141 cgggccgctg cggagatccc cacggcaagc ttcctctctc tgcccgggga gacgcacttc
     1201 tcggcggagc gcgtggcggg cgagctgctg ccgcgtgtcc tggagctgtt ccgttccgcc
     1261 tcggccgacg acgttcggcg gtccccctga ccccggcgcc ccggccgtag gctcgccatg
     1321 gaggatggtc ccgcccgggt ctatggtccg actcgatgaa ggcggatctc ggatcgagca
     1381 gcggaggcta gagtggacgg gcgatgctga gtcgtaccgc gacgtggccc cggccgctgg
     1441 tgttcatgct gctcacggcg gctgggctgt tcgcggcggc ggcccccgcc tgggccgagg
     1501 agccgttccg gctcgagcag caggtcgagg atcgggtcgg cgccctggaa gggcgagaag
     1561 gggaggtccg cgacgcgctg gaacgtctgc aggcgcgcga cggcatccag ctctgggtga
     1621 cctatgtgga caccttttca ggcctccccg cgcaggagtg gacggacgag acggcgacgc
     1681 tcagcagcct gggactgaac gacgtgctgc tcgcggtggc catcgaagac cgagcctacg
     1741 cgtactcggt cgaccagcag ttccccctct ccgatgcgga gttggccgag gtggcgacct
     1801 cgtccatcga gccatcgctg tcggacgacg actgggtcgg cgccgcggtc gcggcggcgg
     1861 agggcatcag ccgcgtgatg aacgagcagc ccgcagcgga cccgggcggt gagaccgagc
     1921 ggccggaggg gacctcctcg cccgtgtgga ccctcctggc cctgctgctc atcggcggga
     1981 tcgcgtacct cgccttcgtc tggggaaagg cacggctgcg ccctcgcgaa cgtccgcggg
     2041 ccgccgcgca agacccgtac gagcggctct ccatcgacga gctccggcag cgagccgccg
     2101 ctcagctcgt ggagacggac gacgccgtga agaccagcga gcaggagctg gccttcgcca
     2161 tcgccgagtt cggagaggag cagacggagc cgttctcggc cgccctcgag gcggcgcggg
     2221 aggacatggc ggtcgcgttc cgactgcgac agcagctcga cgacgccgag cccgaggacg
     2281 agacgaccga gcgatcgatg ctcgtcgaca tctgtcgaag gacggatgcg gccagcgatc
     2341 gcctggactc ggaggccgag aagttcgacg agctacgcga tctccggaag aacgtcccgg
     2401 aggtcctcgc gcgactggaa ggggacgcgg aggccctcgc tcgccggatc cctcaggcgg
     2461 ccgctgtcct gaccgagctc gccgacacgt acgccccgga ggccatggcg gcggtcgccg
     2521 agaacgtgca gcaggtggag gagcgactgg agctcgcgcg ggaggagatc gcctccgggc
     2581 ggggccacct cgagtcgggg gccccgggac tcgccgccgt cgccgcgcgg gctgccgagg
     2641 aggcgacggg tcaggcaacg cggctgctgg acgcggtcga tcgactcaaa caggatctgt
     2701 cggagtcggg ccgtaagatc gaggcggcgg tcgccgacac cgagaccggt ctcgccgagg
     2761 cgagacgcct gaaggccgag cagctgggag acgagatcgc cctggcggag gccgccctcg
     2821 cggaggcgag gagcgccgcc gcgcccgagg ggggc
//
LOCUS       DASUTW010000086         3463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_543446, whole genome shotgun sequence.
ACCESSION   DASUTW010000086 DASUTW010000000
VERSION     DASUTW010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543446"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1005)
                     /locus_tag="VF058_02795"
     CDS             complement(97..1005)
                     /locus_tag="VF058_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6207265.1"
                     /translation="MSIRRGRDAGLVLLLTPLIWGLTFPAGKEALERLPLLPFTAWSR
                     LLGVATVAVALPFLLRRSRFEARRLVVPGVVLGGLMFVAFLLQSAGLERTTATNAGFV
                     TGLYVVFTPVLGLALFRRPVGRAAWLAVGISVVGLALLSVPGLDDLAPRQGDLLVLAS
                     AVVWAGHVVAVGHFAARHPAVPLALAQLVVAAGLHLLATTGEGLHLAAAIEVWPLLVI
                     TGVLGSGVAFTIQVLAQGTVSATRAAIILASESVVAAIAAAIWLGERLDAHQWVGAAL
                     AVAAMVLSEVGARRRPEVRLDPATAV"
     gene            complement(1002..1931)
                     /locus_tag="VF058_02800"
     CDS             complement(1002..1931)
                     /locus_tag="VF058_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207266.1"
                     /translation="MEATPTGRRFGALPVLVAIGIAATTVAQTVTIVRHEPPPPVLLR
                     LNVQKGLAVSYRMEIGMEGRVSAGLFGQPIELTVVMEQDWRVREVGEDGSASIEVDTR
                     LIEASSPQGDVPRSELQEIEGTSTMRLAADGRVLEATLGVTSSLTGAAPIPGLEGGFG
                     PTFPPDPVAPGDRWDLSTEVPLLPGGPSLEVVGEATLDTYEEVGGIRSAVIDSEATMP
                     FDFELDLAALAAQLGAEIPEDEAQGMIAYEGEMTMSSRQWIDPKTGLQVRLEGGGEGD
                     ISFSLGGFPQNGDMPSVPRTNVEFDLTMTLERL"
     gene            complement(1957..>3463)
                     /locus_tag="VF058_02805"
     CDS             complement(1957..>3463)
                     /locus_tag="VF058_02805"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6207267.1"
                     /translation="RTLYRASRVHTLSPYGGGSWLLVDERHVERAGSGEPPQADRVVD
                     LPGTTIIPGFVDAHVHLSATGMKGEGLDLSPAGSKDELLSIVRDYAGDRDGPVFGAGF
                     DEFSWEDPKLPTIAELDGVSLHPIVLVRTDGYLSLANERALELSGALQHEGAERDPAG
                     RPTGLLRKAANSQAQRWYHASLEPAAVQDAQLRAAGLAASRGVTTVQEMAMPDKQGRR
                     DVEVLLGHLQRLPVDCIVYIADMDLPWVMEHGFPRAGGDLTLDGSIGARTAAVLEPYE
                     DGEGSGALQYDDDTLVEFLHNAHLGGLQVGLHVIGDAAIEQALRVWERVYHALDSRGR
                     RHFRARRHRLEHFEMASMQQVERAAGLGLAISIQPVFDAFWGNPGGMYEQRLGSERAW
                     PMNPFRELMMRGLEVGAGSDTPVTPLDPMFGIWALENHHDPAQRMGRDDAIRLFTVGA
                     ARIGHLEKKGRLEPGSSADFAAYEVDPFDVPDPRELRPVLTVSRGREVSAR"
BASE COUNT          505 a         1274 c         1229 g          455 t
ORIGIN      
        1 ggcggcgtga tgggcgcgcc gggcttcaac gcggcgaggg tgatccgccg cgacctccgc
       61 ctccgccgcc gctgaccgaa cgcccggacg cggagctcag accgccgtcg ccgggtcgag
      121 cctgacctcg ggtcggcgcc gcgctccgac ctcggacagg accatggccg cgaccgcgag
      181 cgcggcgccg acccactggt gggcgtccaa ccgctccccc agccagatcg ccgccgcgat
      241 ggccgccacc accgactcgc tcgcgaggat gatcgcggcg cgggtggcgc tgaccgtccc
      301 ctgcgccagc acctggatgg tgaacgccac ccccgatccc agcacgccgg tgattacgag
      361 caggggccac acctcaatcg cggccgccag gtgcaggcct tcccccgtcg tggccaggag
      421 gtgcagccct gcggcgacca cgagctgggc cagcgccagc ggcaccgcgg ggtggcgagc
      481 cgcgaaatgc cccaccgcga ccacgtgccc ggcccacacc acggcggacg ccagcacgag
      541 cagatcgccc tgccgcggcg cgaggtcgtc gagccccggc acgctcagca gcgccaggcc
      601 cacgaccgag atcccgaccg cgagccaggc cgctcgtccc accggacgcc ggaacagcgc
      661 gaggcccagc accggcgtga agaccacgta gagcccggtg acgaagccgg cgttggtcgc
      721 ggtggtccgt tcgaggcccg ccgactggag gaggaaggcg acgaacatca accctccgag
      781 cacgacgcca gggaccacca gtcggcgggc ctcgaacctc gacctccgca ggaggaacgg
      841 cagcgcgacc gcgaccgttg ccacgccgag tagtcggctc cacgccgtga acggcagcag
      901 gggcagcctc tcgagggcct ccttccccgc ggggaacgtc agcccccaga tcaggggcgt
      961 cagcaggagg acgagccccg cgtcccgtcc gcgccggatg ctcacagacg ctcgagcgtc
     1021 atcgtgaggt cgaactccac gttcgtcctg gggacggagg gcatgtcgcc gttctgcggg
     1081 aacccgccga gcgagaagga gatgtcgccc tctccccctc cctccaggcg gacctggagg
     1141 ccggtcttgg ggtcgatcca ctgacgggag ctcatcgtca tctcgccctc gtaggcgatc
     1201 atcccctggg cctcgtcctc ggggatctcc gcgcccagct gggccgccag cgcggccaga
     1261 tcgagctcga agtcgaaggg catcgtggcc tcgctgtcga tcaccgcgga ccggatgcct
     1321 cccacctctt cgtacgtgtc gagcgtggcc tcgccgacga cctccaggga cgggcccccc
     1381 gggaggagcg gcacctccgt gctcagatcc caccgatctc cgggagctac gggatcggga
     1441 gggaaggtgg ggccgaagcc gccctccagg ccggggatgg gggcggcacc cgtgagcgag
     1501 ctcgtgaccc ccagggtggc ctccaggacg cgcccgtccg ccgccaggcg catcgtgctg
     1561 gtgccctcga tctcctggag ctcggaccgc ggcacgtccc cctgcgggct cgaggcctcg
     1621 atcagccgtg tgtcgacctc gatcgaggcc gagccgtcct cgcccacctc ccggacccgc
     1681 caatcctgct ccatgacgac ggtcagctcg atgggctggc cgaacagccc ggccgagacc
     1741 cggccctcca tgccgatctc catccggtag gagacggcca agcccttctg gacgttcagc
     1801 cggagaagca ccggtggggg cggctcgtgg cggacgatgg tgacggtctg ggcgacggtc
     1861 gtggccgcga tgccgatcgc cacgaggacc ggcagcgcgc cgaagcgtct gccggtcggg
     1921 gtcgcctcca tcctcgctca gcctagcatc ggggtctcag cgggcggaca cctcccggcc
     1981 gcgcgagacg gtcaggacgg gacggagctc gcgcgggtcg gggacgtcga agggatccac
     2041 ctcgtaggcc gcgaagtccg ccgaggaccc cggctcgagc ctccccttct tctccaggtg
     2101 gccgatgcgc gcggcaccga cggtgaagag ccggatcgcg tcgtcgcgtc ccatgcgctg
     2161 ggccgggtcg tggtggttct ccagggccca gatcccgaac atcgggtcga gcggcgtcac
     2221 cggcgtgtcg gatcccgcgc ccacctcgag cccgcgcatc atgagctcgc ggaaggggtt
     2281 catcggccag gcccgctcgg aacccaggcg ctgctcgtac atccctcccg ggttccccca
     2341 gaacgcgtcg aagaccggct ggatcgagat ggcgagcccg agcccggccg ccctctccac
     2401 ctgctgcatc gaggccatct cgaagtgctc caggcggtgt cgtcgggcgc ggaagtggcg
     2461 gcgtccgcgc gagtccagcg cgtggtagac gcgctcccag acccggagcg cctgctcgat
     2521 ggcggcgtcg ccgatcacgt ggaggccgac ctggaggccg cccaggtgcg cgttgtggag
     2581 gaactcgacc agggtgtcgt cgtcgtactg gagcgcgccg ctcccctccc cgtcctcgta
     2641 gggctcgagc acggcggcgg tcctggcccc gatcgaaccg tcgagggtga ggtcgccgcc
     2701 ggctcgtggg aagccgtgct ccatcaccca ggggagatcc atgtcggcga tgtagacgat
     2761 gcagtcgacg gggaggcgct ggaggtggcc caggaggacc tcgacgtcgc ggcgtccctg
     2821 cttgtcgggc atcgccatct cctggacggt ggtgacgccc cgcgacgccg ccagccccgc
     2881 cgcccgcagc tgtgcgtcct ggacggcggc gggctcgagc gaggcgtggt accagcgctg
     2941 ggcctgagag ttcgcagcct tccggaggag gccggtcggg cgccccgccg gatcgcgctc
     3001 ggcgccctcg tgctggaggg cgcccgacag ctccaacgcg cgctcgttgg ccaggctgag
     3061 gtagccgtcg gtccgcacca gaacgatggg gtgcagggag acgccgtcca actcggcgat
     3121 cgtcggaagc ttcggatcct cccaggagaa ctcgtcgaag cctgcgccga agacggggcc
     3181 gtcccggtcg cccgcgtagt cgcggacgat gctcagcagc tcgtccttcg agcccgccgg
     3241 cgagaggtcg agcccctcgc ccttcatccc cgtggccgag aggtgaacgt gggcgtccac
     3301 gaagccgggg atgattgtcg tcccggggag atcgacgacg cggtcggcct ggggcggttc
     3361 gcccgatccg gcccgctcca cgtggcgctc gtccacgagc agccacgagc ccccgccgta
     3421 gggcgacagt gtgtggacgc gtgacgctcg atagagggtt cgc
//
LOCUS       DASUTW010000087         2262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1581013, whole genome shotgun sequence.
ACCESSION   DASUTW010000087 DASUTW010000000
VERSION     DASUTW010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581013"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="VF058_02810"
     CDS             complement(<1..988)
                     /locus_tag="VF058_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase small subunit domain-containing
                     protein"
                     /protein_id="HEX6207268.1"
                     /translation="MSEVEILEVAGKDVRVTSTGKVLFPQRGITKLDLVRYYLSVGEG
                     AVRGARDRPVILHRFPHGIDDEDFYQKRVQKGSAGWLVTARIAFPSGRTAEELVIADE
                     AHLAWMINLGCIDINPWPVRAGDLDHPDELRVDLDPTPGIPWGHVREVALLAREVLEE
                     HGLAGYVKTSGKRGIHVLVRIEPRWTFTAVRRAALALAREVERRDARATTAWWKEERT
                     GVFVDYNQNARDRTVASAYSVRPVPDARVSAPLTWDEVPDVEPEDLTIDSVPGRYAEI
                     GDPHEPIDDRPGSLDPLLELAERDRRGGLGDAPWPPHFPKGDDEPVRAAPSRR"
     gene            1053..1214
                     /locus_tag="VF058_02815"
     CDS             1053..1214
                     /locus_tag="VF058_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207269.1"
                     /translation="MPLAIVAHAGWDELLVAVVAVGIVLILRTRSERKRRLEEGGEER
                     APEPAEEPR"
     gene            1211..1753
                     /locus_tag="VF058_02820"
     CDS             1211..1753
                     /locus_tag="VF058_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207270.1"
                     /translation="MTSRRALLGLTAAGVVVGHSIAYVLAAADHAPGAHRYWPVAVAV
                     AVVAGITASVRLTLPHLREASLAPTPVLPFAARLATLQVLLFVVMEITERLAGGQSAH
                     GFLASDLAWIGVAIQVLVALAVALLTRLLGQVAGAIGRALRRVWDQPIPLPLPRAVPV
                     LRPQVLAGSGSMRGPPPSLD"
     gene            1787..>2262
                     /locus_tag="VF058_02825"
     CDS             1787..>2262
                     /locus_tag="VF058_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207271.1"
                     /translation="MSRIRTGAALAVAGAISMLLLLAPAPAMAHEGREVGDVVMVVGF
                     GSEPAMAYEPNWASLTLTDHDMEEPILNGVDLEVEITYGEESTTMAMEPAFGDTGEYR
                     AVFVPSRPGDYSFRFFGTVRGQRINETFSSGPDTFSPANDPAELSFPAQDPTVAEVA"
BASE COUNT          278 a          827 c          818 g          339 t
ORIGIN      
        1 ggcgacgcga cggggccgcc cggacgggct cgtcgtctcc cttggggaag tggggcggcc
       61 acggcgcgtc gccgaggccc ccgcgccggt ctcgttcagc gagctcgagg agcggatcga
      121 gcgagccggg tcggtcgtcg atcggctcgt gggggtcacc gatctccgcg tagcggccgg
      181 ggaccgagtc gatcgtcagg tcctcgggct ccacgtcggg cacctcgtcc caggtcaggg
      241 gggccgagac gcgggcgtcc ggcacgggcc gcaccgagta ggcggaggcc accgtccggt
      301 ccctcgcgtt ctggttgtag tcgacgaaga cgccggtccg ctcctccttc caccaagcgg
      361 tcgtggcccg ggcgtcgcgg cgctcgacct cgcgagcgag ggcgagcgcc gcgcgccgca
      421 ccgccgtgaa cgtccacctc ggctcgatcc ggaccaggac gtggatccct cgcttccccg
      481 aggtcttgac gtagccggca agcccgtgct cctcgagcac ctcgcgagcc aggagcgcca
      541 cctcgcggac gtgcccccag gggatccccg gcgtcgggtc gaggtccacg cgcagctcgt
      601 ctgggtggtc gaggtcgccg gcccgcacgg gccaggggtt gatgtcgata caccccaggt
      661 tgatcatcca ggccaggtgc gcctcgtcgg cgatcaccag ctcctcggcc gtcctgccgc
      721 tggggaaggc gatgcgggcc gtgacgagcc agccggccga ccccttctgc acccgcttct
      781 ggtagaagtc ctcgtcgtcg atcccgtggg ggaaccggtg aaggatcacc gggcggtcac
      841 gggccccgcg gaccgccccc tctcccaccg agaggtagta gcggacgagg tcgagcttcg
      901 tgatcccccg ctgggggaag agcaccttgc ccgtgctggt gacgcgcacg tccttgccgg
      961 cgacctcgag gatctcgacc tcgctcaccg gacattccta ccacgccggg ccgcctctcc
     1021 cttggtcggc gactgacgct aggatggctg ccgtgcctct tgcgatcgtg gcccacgcgg
     1081 gctgggacga gctgctcgtc gccgtggttg cggtggggat cgtgctgatc ctccgcaccc
     1141 ggtcggagcg gaagcgccgc ctggaggagg gcggcgagga gcgggcgccc gagcccgcgg
     1201 aggaacctcg atgacctccc ggcgcgccct gctcggcctg acggcggccg gcgtcgtggt
     1261 ggggcactcg atcgcctacg tcctggccgc ggccgaccac gcccccggag ctcaccgcta
     1321 ctggccggtc gcggtggcgg tggccgtggt ggcggggatc acggcctcgg tccgcctgac
     1381 cctcccccac ctgcgcgagg cctcgctcgc gcccaccccc gtgctgccct tcgccgctcg
     1441 cctggccacc ctccaggtcc tgctcttcgt ggtgatggag atcaccgagc gcctggccgg
     1501 cggccagagc gcccacgggt tcctggccag cgacctggcg tggatcggcg tcgccatcca
     1561 ggtcctggtg gccctggccg tcgcgctgct gacccgcctt ctgggccagg tggccggcgc
     1621 gatcggccgg gcgctgcggc gcgtctggga ccagccgatc ccgctgcccc tcccccgggc
     1681 cgtgcccgtc ctccgccccc aggtgctggc gggatcgggc tcgatgcgag gcccccctcc
     1741 ttcgttggac tgacgcatcg tccaacgaca cgaagggagg gatcctatgt cacgcatccg
     1801 aacgggtgcg gcgctggcgg tggccggcgc gatctcgatg ctgctcctgc tcgcccccgc
     1861 gccggcgatg gcgcacgagg gccgcgaggt gggcgacgtc gtgatggtgg tggggttcgg
     1921 gagcgagccg gccatggcct acgagcccaa ctgggcctcg ctgacgctga ccgaccacga
     1981 catggaggag ccgatcctaa acggcgtgga cctcgaggtg gagatcacct acggggagga
     2041 gtccacgacg atggccatgg agcctgcgtt cggggacacc ggggagtacc gggccgtctt
     2101 cgtgccgtcg cggcccggag actactcgtt ccggttcttc ggcacggtcc gtggccagcg
     2161 gatcaacgag acgttcagct ccggccccga caccttcagc ccggcgaacg acccggcgga
     2221 gctctcgttc cccgcacagg acccgaccgt ggccgaggtg gc
//
LOCUS       DASUTW010000088         6711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_414787, whole genome shotgun sequence.
ACCESSION   DASUTW010000088 DASUTW010000000
VERSION     DASUTW010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6711
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414787"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1779)
                     /locus_tag="VF058_02830"
     CDS             complement(<1..1779)
                     /locus_tag="VF058_02830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6207272.1"
                     /translation="MSEYPAHREADVVLRDGSTVRIRPVRPEDREAILVFLKGLSDEA
                     RWLRFFSGFPDLDRAAESAAKVDYHDRFGLLALVGDTVVGHSTYLADGDRAEVGFAVA
                     DAYQGRGLGTIMLGQLAQAAAENDIHTFEAEVMPENRRMLEVFRDSGFPIETRTEPGL
                     IKAEFPTSLTGEALERFDRREQVAATNAMRSFLSPSAVAVIGASRTRGTIGAETFHNL
                     LVTGFEGAVYPVNPNAPVVQSVAAYPSIKDVPDQVELAVVVVPASAVVPVAKECAVAG
                     VRSLVVISSGFAEVGEEGSELQRELLAVCRESGMRLIGPNCMGIMNTDPEVRLDASFA
                     PTFPPHGRVGFLSQSGALGLAVIGRAVELGLGLSSFVSVGNKADISGNDLLNYWEADE
                     GTDVILLYLESFGNPRRFSRVARRVGRSKPIVAVKSGRTAAGARATSSHTGALIAASD
                     VTVEALFHQTGVIRTDTLEELFDVATLLSSQPLPAGKRVGIITNAGGLGILCADACEA
                     GGLEVPTLSDEAVRKLREFLPAEASVQNPVDMIASASAEDYRKTIEIVAGEGGVDAII
                     VIFIPPLLTRAEDVAHCIRGAADRIDE"
     gene            1864..2238
                     /locus_tag="VF058_02835"
     CDS             1864..2238
                     /locus_tag="VF058_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HEX6207273.1"
                     /translation="MDLDEMLKDARDAITVGRVYGEPFEKNGVTILPAASVRGGGGGG
                     SGEGGGPEGEGKGTGGGFGVTARPVGAYVIRGEDVRWEPAVDVTRMALMGQAVALIAL
                     LVIRSIVKSRAKAKARIAKAES"
     gene            complement(2235..3041)
                     /locus_tag="VF058_02840"
     CDS             complement(2235..3041)
                     /locus_tag="VF058_02840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX6207274.1"
                     /translation="MLSFDQGFFGPGSVSWQVHGELTVLFGGARSLLMQSAHPLVIAG
                     ARETGFYRRSPWKRLQRTLMLNYAITFGTKKDARAAAEHINEVHRRVHGIDPVTGQAY
                     HGLDPDLLLWVHATLVHSALLFEHLTVGKLDPAGRQRFHEEQMMVAEMLELPREKIPP
                     TVEELSEYMRDVMAGDTLIVTDAAFEVANLFREFPPEAEWKPVLRAISFWSFATLPPK
                     LRQAYGVRWSPARDMVARASLAALKRLRPAIPRRYRRILIAEQARIRTSG"
     gene            3082..3963
                     /locus_tag="VF058_02845"
     CDS             3082..3963
                     /locus_tag="VF058_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207275.1"
                     /translation="MELLAATDEGVISLPGGERRGAEPVTFLHRAGGVTVEVRGGHEV
                     VVRSNGEAPGLRSDLRANCALALAGGGALVGTAEARLFLARNGSLEAVEDFDRAEGRE
                     RWHTPWGGPPDVRSLSAGPDGMIYANVHVGGILRSDDGGETWAPTAIPIEEDVHQVLA
                     HPERPGLVLAATAYGLARSEDGGASWETIDEGLERRYSRAVAVAGETVLVSASAGPGG
                     GQAALYRLAGDRFVKAGLPDPFGSNLDTHRLVAAGTTAAAALPDGEAHVSEDAGGAWE
                     RVAASLPSVHCLLLEER"
     gene            3960..4943
                     /locus_tag="VF058_02850"
     CDS             3960..4943
                     /locus_tag="VF058_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX6207276.1"
                     /translation="MNVPKPRDPLIRRLLWPAALLIGVTTYGVGGYMLLEGWSFIDAL
                     YMTVITLSTVGFREVQPLDATGRAFTLSLVVLGVTAVLVGLTLIGIWISEGNLGERRR
                     RKRMKHVIDRLEGHFVVCAFGRVGRTVARRFLDAGVPFVVVDRDEALEARMVDMNVPH
                     LIGDPSDEEVLHQAGIDRARGLVCAVDSDATNVFITLTARALKPDLFIVARAATSESI
                     PRLEKAGADRVVSPYVTSGEAMALMSLRQDVVGMLDVADLGDRDVRVEELVVSAGSHL
                     EGLRVKEAAGQALALALRHADGSTTVGPSADRPLRAGDRLLLMADERTLQA"
     gene            complement(5005..5622)
                     /locus_tag="VF058_02855"
     CDS             complement(5005..5622)
                     /locus_tag="VF058_02855"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6207277.1"
                     /translation="MRDVMTVGVVTAAPDTPVKELAALMVGARISGIPVVEDGKVVGI
                     VSESDLLPLHEGDPGHRVQVAGDVMTRKLISLVEGMTVTEAARVLQRHGVKRAPVMRG
                     ERLVGIVTRANLLRPYLRTDLEIQAEVEEAVLSGALGISPRGVRALVIDGVVRLEGRV
                     TSARTRAILLRLVRAVDGVVDLEEHLELEEEPNGHSVQEEAERAL"
     gene            complement(5646..6383)
                     /locus_tag="VF058_02860"
     CDS             complement(5646..6383)
                     /locus_tag="VF058_02860"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6207278.1"
                     /translation="MKKLKVEDVMTRTVVVVREGTPFKDVVRMMLEHRVSGVPVLDGE
                     GRLTGIVTEADLLTLEEGKVEPKHRRSFLEWLVQPGRLAEIEAEAAEVRAADIMTPSV
                     VTARPELTVPEAAKLMLDAQVKRLPVIDEDGKVLGIVSRRDLLQPFLRTDEEIEREIR
                     EEVIFRTMWIDPDTIGVEVERGCVRLRGTVERRSVAEILVELVHRVEGVVGVEDELGY
                     RWDDRKVKPRGPFSAPRWSENWARRGA"
     gene            complement(6386..>6711)
                     /locus_tag="VF058_02865"
     CDS             complement(6386..>6711)
                     /locus_tag="VF058_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6207279.1"
                     /translation="DAAGRMQYQEIGSLAVLEHGKLVGILTERDLARALADGVDPAET
                     PVGAYMTEEPVTVTPEADAASVAATMLDLGVRHIPVMEGGQVIGMVSSRDLLSLEAWN
                     AIGVT"
BASE COUNT          961 a         2438 c         2337 g          975 t
ORIGIN      
        1 ctcgtcgatc ctgtccgcgg ccccacggat gcagtgggcc acgtcctcgg cccgggtgag
       61 gagcggaggg atgaagatca cgatgatcgc gtcgacgccg ccctcccccg ccacgatctc
      121 gatcgtcttg cgatagtcct cggcgctggc cgaggcgatc atgtccaccg ggttctgcac
      181 cgacgcctcg gccgggagga actccctcag cttcctcacg gcctcatcgc tgagggtcgg
      241 aacctccagg ccccccgcct cgcacgcgtc ggcgcagagg atccccagcc cgcccgcgtt
      301 cgtgatgatc cccacccgct tcccggccgg cagcggctgg ctcgacagga gcgtggcgac
      361 gtcgaacagc tcctcgagcg tgtcggtccg gatcaccccc gtctggtgga agagcgcctc
      421 gaccgtcacg tccgacgccg cgatcagcgc gccggtgtgt gacgaggtgg ctcgggcccc
      481 cgccgcggtc cgcccgctct tcaccgccac gatcggcttc gagcggccga ccctccgagc
      541 cacccgagag aaccgtcgag ggttcccgaa cgactcgagg tacagcagga tcacgtcggt
      601 gccctcgtcg gcctcccagt agttgaggag gtcgttcccg gagatgtcgg ccttgttgcc
      661 caccgagacg aaggacgaga ggcccagccc gagctcgacg gcgcgcccga tcaccgccag
      721 tcccagggcg ccgctctggg agaggaatcc cacgcgcccg tgcggcggga acgtgggggc
      781 gaaggacgcg tccagcctca cctcggggtc ggtgttcatg atccccatgc agttcgggcc
      841 gatcagccgc atccccgact cccgacagac cgccagcagc tcccgctgca gctcgctgcc
      901 ctcctccccc acctcggcga agccggacga gatcaccacc agcgagcgca cccctgccac
      961 ggcgcactcc ttcgccaccg gaacgaccgc cgacgccggg acgaccacca cggccagctc
     1021 gacctggtcg ggcacgtcct tgatcgatgg gtaagccgcc accgactgca cgacgggcgc
     1081 gttggggttc accgggtaga cggcgccctc gaagccggtc acgaggaggt tgtggaacgt
     1141 ctcggccccg atcgtcccgc gggtccgcga ggcaccgatc accgccacgg ccgaggggga
     1201 caggaacgac cgcatcgcgt tcgtcgccgc cacctgctcc cgacggtcga agcgctcgag
     1261 cgcctctccg gtcagcgagg tcgggaactc ggccttgatc agccccggct cggtgcgggt
     1321 ctcgatcggg aagccgctgt cgcggaagac ctcgagcatc cggcggttct cgggcatgac
     1381 ctcggcctcg aaggtgtgga tgtcgttctc ggccgcggcc tgggccagct ggcccagcat
     1441 gatggtgccc aggcccctcc cctggtaggc gtcggccacg gcgaagccga cctcggccct
     1501 gtcgccgtcg gccaggtacg tggagtggcc gaccacggtg tcgcccacca gggccagcag
     1561 gccgaagcga tcgtggtagt cgaccttcgc ggcgctttcg gcggcgcggt cgaggtcggg
     1621 gaagccggag aagaagcgca gccagcgcgc ctcgtcgctg aggcccttga ggaagacgag
     1681 gatcgcctcg cggtcctcgg gacggaccgg acggatgcgg accgtggacc cgtcccgcag
     1741 gacgacgtcg gcctcgcggt gcgcggggta ctcgctcatc ccggggtcca tcctacccgc
     1801 ggcgccgctc gaccctgacc ccttccggga caggggggta cggtggaggc aggaggtgat
     1861 cccatggatc tggacgagat gctgaaggat gcacgcgacg ccatcacggt gggtcgcgtc
     1921 tacggcgagc ccttcgagaa gaacggcgtg acgatcctgc ccgctgcctc ggtgcgcggc
     1981 ggcggaggcg ggggcagcgg tgagggcggc ggtcccgagg gcgagggcaa gggcaccggc
     2041 ggggggttcg gcgtgacagc gcgcccggtc ggcgcctacg tgatccgcgg cgaggatgtg
     2101 cgctgggagc ccgccgtgga cgtgacgcgc atggcgctca tgggccaagc ggtggcgctg
     2161 atcgccctgc tcgtgatccg gtcgatcgtg aagtcgaggg cgaaggccaa ggcccggatc
     2221 gcgaaggccg agagctagcc ggaggtccgg atgcgtgctt gctcggcgat caggatgcgc
     2281 cggtagcgcc gggggatcgc cgggcggagg cgcttcaacg ccgccaggct ggcccgggcc
     2341 accatgtccc gggcggggct ccatcggacc ccgtaggcct ggcgcagctt cgggggcagc
     2401 gtggcgaacg accagaagga gatggcgcgc aggaccggct tccactccgc ctcgggcggg
     2461 aactcgcgga acaggttcgc cacctcgaac gcggcgtcgg tcacgatcag cgtgtcgccg
     2521 gccatgacgt cgcgcatgta ctccgagagc tcctccaccg tgggcgggat cttctcgcgg
     2581 ggcagctcca gcatctccgc aaccatcatc tgctcctcgt ggaagcgctg acgccccgcc
     2641 gggtcgagct tccccaccgt gaggtgctcg aagagcagcg cgctgtggac gagcgtggca
     2701 tggacccaca ggaggaggtc gggatcgagg ccgtggtagg cctggccggt caccggatcg
     2761 atcccgtgga cgcgacggtg gacctcgttg atgtgctcgg cggcggccct ggcgtccttc
     2821 ttcgtcccga acgtgatggc gtagttgagc atcagcgtgc gctggaggcg cttccagggg
     2881 ctgcggcggt agaagccggt ctcgcgggcc cccgcgatca cgagcgggtg ggcagactgc
     2941 atcaggaggc tgcgagcgcc accgaagagc acggtcagct ccccgtggac ctgccacgag
     3001 acgctgcccg gtccgaagaa cccctggtcg aacgagagca cgagggcatg gtacgccccg
     3061 cgcggcgcta ggctgacggc catggagctg ctggcggcca ccgacgaggg cgtgatctcg
     3121 ctgcccggcg gcgagcggcg gggcgccgag cccgtcacct tcctccaccg cgccggcggg
     3181 gtcacggtcg aggtgcgagg gggccacgag gtggtcgtgc ggtcgaacgg cgaggccccc
     3241 gggctccgct cggatctccg cgccaactgc gcgctggccc tggccggcgg cggcgcgctc
     3301 gtgggaacgg ctgaggcccg tctgttcctg gcgcggaacg ggagcctcga ggccgtggag
     3361 gacttcgatc gagccgaggg gcgcgagcga tggcacacgc cctggggcgg gccgcccgac
     3421 gtccgctcgc tcagcgccgg gcccgacggc atgatctacg ccaacgtcca cgtgggcggg
     3481 atcctccgct ccgacgacgg gggcgagacg tgggcgccca ccgcgatccc gatcgaggag
     3541 gacgtccacc aggtgctggc ccaccccgag cgtccgggcc tggtgctggc ggccaccgcc
     3601 tacgggctcg ccaggagcga agacggaggc gcgagctggg agacgatcga cgaggggctg
     3661 gagcgtcggt acagccgagc ggtggcggtc gccggcgaga cggtgctcgt gagcgcctcc
     3721 gccggacccg gcgggggaca ggccgccctc taccgtctcg ccggcgaccg cttcgtcaag
     3781 gcgggcctcc ccgacccctt cgggagcaac ctcgacaccc accggctggt cgcggcgggc
     3841 acgaccgcgg cggcggccct ccccgatggg gaggcccacg tctccgagga cgcgggagga
     3901 gcctgggagc gggtcgcggc atcgctgccc tccgtccact gtctcctgct ggaggagcgg
     3961 tgaacgtccc caagccgcgc gaccctctga tccgccgtct cctctggccg gccgccctcc
     4021 tgatcggcgt caccacctac ggcgtcggcg gctacatgct cctggagggc tggtcgttca
     4081 tcgacgccct ctacatgacc gtcatcacgc tgagcaccgt ggggttccgg gaggtccagc
     4141 ccctcgacgc gaccggtcgg gccttcaccc tcagcctggt ggtcctcggt gtcaccgcgg
     4201 tgctggtggg cctcaccctc atcgggatct ggatctccga ggggaacctc ggcgagcgac
     4261 ggaggaggaa gcggatgaag cacgtgatcg atcgccttga gggccacttc gtcgtctgcg
     4321 ccttcggtcg agtgggcagg accgtggccc gacgcttcct ggacgcgggg gtcccgttcg
     4381 tcgtcgtgga tcgggacgag gccctggagg cccggatggt ggacatgaac gtcccccacc
     4441 tgatcggaga tccttccgac gaagaggtcc tccatcaagc ggggatcgac cgcgctcgcg
     4501 gcctcgtctg cgccgtggac tcggacgcga cgaacgtctt catcacgctg acggcccggg
     4561 ccctcaagcc ggatctcttc atcgtggcca gggcagcgac ctcggagtcg atcccacgat
     4621 tggagaaggc gggggccgat cgagtcgtct ccccgtacgt gaccagcggc gaggccatgg
     4681 ccttgatgtc cctccgccag gacgtcgtcg ggatgctcga cgtggccgac ctcggggacc
     4741 gcgacgtgcg ggtggaggag ctcgtggtga gcgcggggtc gcatctcgag ggcctccggg
     4801 tgaaggaggc ggccggccag gcgctcgccc tggcgttgag acacgccgac ggctccacca
     4861 ccgtggggcc gtcggccgac cggccgctga gggcgggcga ccgcctcctg ctcatggccg
     4921 acgagcggac gctccaagcc taggaacacg gagcgagggc ggcccttggt ggccgccctc
     4981 gctggtgcac catctcctcg ggcctcacag ggcccgctct gcctcctcct gaacggaatg
     5041 gccgttcggc tcctcctcga gctccaggtg ctcctcgagg tccaccacac catccacagc
     5101 ccgcacgagc cgaaggagga tggcgcgggt ccgggcgctc gtcacacgac cttcgagcct
     5161 cacgaccccg tcgatgacga gcgcccggac ccccctcggg ctgatcccga gcgcccccga
     5221 caggaccgcc tcctcgacct ccgcctggat ctcgaggtcg gtccgcaggt acgggcgcag
     5281 caggttcgcc ctcgtgacga tcccgaccag acgctccccc cgcattaccg gcgcgcgctt
     5341 cacgccgtgg cgctggagga cgcgcgccgc ctcggtcacc gtcatcccct cgacgagcga
     5401 gatgagcttc ctcgtcatca cgtcgccggc cacctgcacg cgatggcccg ggtcgccctc
     5461 gtggaggggc agcaggtccg actcggagac tatgccgacg accttcccgt cctccacgac
     5521 cgggatgccg ctgatccgcg cgcccaccat gagcgctgcc agctccttga cgggggtgtc
     5581 cggggcggcc gtcaccaccc ccacagtcat cacgtccctc acgagcatct ggtcggtccc
     5641 ccctctcagg ctcctcgtcg agcccagttc tcgctccagc gaggagccga gaagggtccc
     5701 cgtggcttga ccttgcggtc gtcccaccgg tacccgagct cgtcctcgac gcccaccacg
     5761 ccctcgacgc ggtggaccag ctccacgagg atctcggcca cgctcctccg ctccaccgtc
     5821 ccgcggagtc gcacgcatcc ccgctcgacc tcgaccccga tcgtgtccgg gtcgatccac
     5881 atcgtgcgga agatgacctc ctcgcggatc tcgcgctcga tctcctcgtc ggtccggagg
     5941 aacggctgga gcaggtcgcg gcggctgacg atgccgagga ccttcccgtc ctcgtcgatg
     6001 acggggagcc gcttcacctg ggcgtcgagc atcagcttcg ccgcctccgg caccgtcagc
     6061 tccgggcgcg cggtcacgac gctcggcgtc atgatgtcgg cggcccggac ctccgcggcc
     6121 tccgcctcga tctcggcgag acggccgggc tgcaccagcc actccaggaa cgagcggcgg
     6181 tgcttcggct cgaccttccc ctcctcgagg gtcagcaggt cggcctcggt gacgataccc
     6241 gtcaggcgcc cctctccatc gagcaccggc acaccgctca cgcggtgctc cagcatcatc
     6301 cggacgacgt ccttgaacgg cgtgccttct cggacgacga cgaccgtccg cgtcatcacg
     6361 tcctcgacct tcagcttctt catcgtcacg tcactccgat cgcgttccag gcctcgaggg
     6421 acaggaggtc ccgcgaggac accatcccga tcacctggcc cccctccatc accgggatgt
     6481 gacggacccc gaggtcaagc atcgtcgcag ccaccgaggc ggcgtctgcc tcgggagtca
     6541 ccgtcaccgg ctcctcggtc atgtaggccc cgaccggcgt ctccgcgggg tccacgccgt
     6601 cggcgagggc gcgcgcgagg tcacgctcgg tgaggatgcc cacgagcttc ccgtgctcga
     6661 gcacggccag ggacccgatc tcctggtact gcatgcggcc cgcggcgtcg a
//
LOCUS       DASUTW010000089         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_545007, whole genome shotgun sequence.
ACCESSION   DASUTW010000089 DASUTW010000000
VERSION     DASUTW010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545007"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VF058_02870"
     CDS             <1..534
                     /locus_tag="VF058_02870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEX6207280.1"
                     /translation="LRRLLVGGWAMMQTRALSVTETGRLGELYERHAPDAVRLGYLIT
                     GDASLAEDLVHEAFVKLLGRFRDLRNPDAFHWYLHKTVVNLARSHFRHARVEREYVER
                     EGRRPVAAADPDPMERDEMWAALGRLSERQRAAIVLRYYEDLTEAQTAEVLGVRLGTV
                     KSLVSRGIERLREEMGR"
     gene            531..1310
                     /locus_tag="VF058_02875"
     CDS             531..1310
                     /locus_tag="VF058_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207281.1"
                     /translation="MTDFHDELREALSRKAAEAPRPGGMPEDVLRRAKRRRVANGALA
                     GGLALALAFGGFIGVRAAMDAAAPVREEVPLGPGDETPSPEPTAAPSPEPTESPEPDP
                     TGSPWPAPVTSCARTEAGSDAVFPDFVDLSIQEPDVVPGRASITFHFEPMAGQTEAPW
                     HIIDFTDQLVTDGEGAPVDVQGETFVAVTFMARGVSLDGEEIVEIYTGPKEFRLDGPI
                     MLEVEQTGDFEGMVSWGIGLSEKTCYAVDAGPDHLTITFVR"
     gene            1321..1944
                     /gene="pdxH"
                     /locus_tag="VF058_02880"
     CDS             1321..1944
                     /gene="pdxH"
                     /locus_tag="VF058_02880"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX6207282.1"
                     /translation="MDEVRPDPPEAFDGGSAPSNPMERFRRWWEEARTLLGEEADIMV
                     VATAGADGRPSARNVLLRGLGPKSFVFYTNLRSRKARDLAATSYAALTLYWRDIGRQV
                     RVEGPVERTSRDEDEAYWRTRPIGAQVAAWVSAQSEPLTSREELTRAYDEALRRFGQD
                     VPLPDHWGGYRVIPEAMEFWQGREHRLHDRIRYERRGNAWTAHRLAP"
BASE COUNT          316 a          709 c          801 g          261 t
ORIGIN      
        1 ctgaggcgtc ttctggtcgg aggatgggcc atgatgcaga cgagggcgtt gagcgtgacc
       61 gagacgggga ggctggggga gctgtacgag cggcacgccc ccgacgccgt ccggctcggg
      121 tacctgatca ccggcgacgc ctcgctggcc gaggatctcg tccacgaggc gttcgtcaag
      181 ctgctgggtc ggttccgcga cctgcggaac ccggacgcgt tccactggta cctccacaag
      241 acggtggtga acctcgcgcg gtcgcacttc cgccacgcga gggtggagcg cgagtacgtc
      301 gagcgggagg ggcggcggcc ggtcgcggcc gcggacccgg acccgatgga gcgggacgag
      361 atgtgggcgg cgctcggaag gttgtccgag cgccagcgcg ccgcgatcgt gctccgctac
      421 tacgaggatc tgaccgaggc gcagacggcc gaggtgctgg gcgtccgtct gggcacggtg
      481 aagtcgctgg tctcgcgagg gatcgagcgg ctgcgggagg agatgggacg atgaccgact
      541 tccacgacga gctgcgcgag gcgctgagcc gcaaggccgc ggaggcgccg cggcccggcg
      601 ggatgcccga ggacgtgctg cggagggcga agcggcgccg ggtggccaac ggggcgctgg
      661 cgggcgggct ggccctggcc ctcgccttcg gcgggttcat cggggtgcgc gccgcgatgg
      721 acgcggcggc gccggtgcgg gaggaggttc cgttggggcc gggcgacgag acgccgagcc
      781 ccgagcccac ggccgcgccg tcccccgaac ccacggagag cccggagcca gacccgacag
      841 gcagcccctg gccggcaccg gtgacctcct gcgcgcggac ggaggcgggc agcgacgcgg
      901 tgtttcccga cttcgtggac ctctcgatcc aggagcccga cgtggttccg ggccgggcga
      961 gcatcacgtt ccatttcgag cccatggcgg ggcagacgga ggccccgtgg cacatcatcg
     1021 acttcaccga ccagctggtg accgatggtg agggcgcgcc ggtggacgtc cagggggaaa
     1081 cgttcgtcgc ggtgaccttc atggcgcggg gcgtgagcct cgatggcgag gagatcgtgg
     1141 agatctacac cgggcccaag gagttccggc tcgacggccc gatcatgctc gaggtcgagc
     1201 agaccgggga cttcgagggg atggtctcct gggggatcgg gctctccgag aagacctgct
     1261 acgcggtcga cgcgggaccg gaccacctga cgatcacctt cgtccggtag cctccctggg
     1321 gtggacgagg ttcgccccga tccccccgag gccttcgacg gcggaagcgc tccatcgaac
     1381 ccgatggagc gcttccgccg ctggtgggag gaggcccgca ccctgctggg cgaggaggcc
     1441 gacatcatgg tcgtggccac cgccggggcc gacggccggc cctcggcccg caacgtgctg
     1501 ctccggggcc tcggccccaa gagcttcgtc ttctacacga acctccgcag ccggaaggcc
     1561 cgcgacctgg ccgccacctc gtacgccgcg ctgacgctct actggcggga catcggcagg
     1621 caggtgcggg tggagggccc ggtcgagcga acctcccgcg acgaggacga ggcctactgg
     1681 cggactcggc ccatcggggc ccaggtcgcg gcatgggtct cggcccagag cgagccgctc
     1741 accagccgcg aggagctcac ccgagcctac gacgaggccc tccgccggtt cggccaggac
     1801 gtgccactcc ccgaccactg gggcggctac cgagtgatcc ccgaggcgat ggagttctgg
     1861 caggggcggg agcaccgcct ccacgaccgg atccgctacg agcgccgcgg caacgcctgg
     1921 accgcccacc gcctggcgcc gtagcggccg gagccgcgaa ggaccgggcc gggggggtgc
     1981 cccgcaccaa ccggaccaac ggccgagcat gacagaccca cgtgatacag gacgccgatg
     2041 gacctcaccc cagagcagtg gcgcgagctc gaggcgatag gcctgcc
//
LOCUS       DASUTW010000090         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1192630, whole genome shotgun sequence.
ACCESSION   DASUTW010000090 DASUTW010000000
VERSION     DASUTW010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192630"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VF058_02885"
     CDS             <1..682
                     /locus_tag="VF058_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX6207283.1"
                     /translation="AVPASEWQSKKARDLLKLLVARRGRPAPREFLMEVLWPEEDPDK
                     VANRLSVALTTVRTVLDPERGRPQDHFVDADREAVALEIDRLDVDVERFLADGTAALE
                     LVQGGSTEEALPLLREAEAAYAGDFLEENLYDDWAVALREEARATYLSVCRELARLAV
                     AENDHDAAIRYLLRIIERDPFEERAHLGLVTVLTSAGRHGEARRAYRIYRSRMEELAV
                     EPAPFPSA"
     gene            725..916
                     /locus_tag="VF058_02890"
     CDS             725..916
                     /locus_tag="VF058_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207284.1"
                     /translation="METFVVRVWPHEEADGLHGVVRHVRSGREVRFSNATQLADLLRL
                     GFPPEAPEPTGPPEEPAAR"
     gene            complement(927..1373)
                     /locus_tag="VF058_02895"
     CDS             complement(927..1373)
                     /locus_tag="VF058_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207285.1"
                     /translation="MRLLRGTLYAHALAWIVLGTLLAIWPGLLFSLLGQPRYPDYAWV
                     RLVGVQAVGLALFMVLVGHRLEELWWWAWGFVLTTGAVAAVLTLNAAFGLPRGADAVL
                     WWALAIGHWVLGSVTLIGMSRAARERPTDRYEPAPARDPHPRRRRG"
     gene            1510..>2190
                     /locus_tag="VF058_02900"
     CDS             1510..>2190
                     /locus_tag="VF058_02900"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6207286.1"
                     /translation="MTGPRIAVIGLDCATPLLLFDRLRSETPNIQALLGRSMHGELES
                     ITPPITVPAWACSMTGKTPGQLGIYGFRNRKDTSYDGLSIATSLAVKEPAVWDQLGEA
                     GMRSLLIGVPPAYPIKPVEGWRVSCFLTPPSADQYTHPADLKAEVEEVVGDYIFDIPN
                     FRQQGLDFVLSQSHEMTKKRFALARHMVRTKPWDFFMMVEMGTDRLHHVFWQYCDPDH
                     PKYEPGNPY"
BASE COUNT          351 a          790 c          761 g          288 t
ORIGIN      
        1 ggccgtcccc gcctccgagt ggcagtcgaa gaaggccagg gacctcctga agctcctggt
       61 ggcgaggcgg ggacggcccg ccccccgcga gttcctcatg gaggtcctgt ggcccgagga
      121 ggatcccgac aaggtggcca accgcctctc ggtggccctc accaccgttc ggaccgtgct
      181 cgatccggag cgggggagac cccaggacca cttcgtggac gccgaccgcg aggccgtagc
      241 gctcgagatc gaccgcctgg acgtcgacgt cgagcgcttc ctggccgacg ggacggccgc
      301 cctcgagctc gtccagggag ggtcgaccga ggaggccctc cccctcctgc gcgaggccga
      361 ggcggcctac gcgggcgact tcctcgagga gaacctctac gacgactggg ccgtggcgct
      421 gcgggaggag gcgagggcca cctacctgag cgtgtgccgg gagctggcgc gcctcgcggt
      481 cgcggagaac gatcacgacg cggcgatccg gtacctcctc cgcatcatcg agcgggatcc
      541 gttcgaggag cgggcgcacc tcggcctcgt gaccgtcctg acgagtgcgg ggaggcacgg
      601 ggaggccagg cgggcctacc gcatctaccg gagccggatg gaggagctcg cggtggagcc
      661 ggcgcccttc ccttcggcgt aggaccttca gggaacccga aggatcccgt gtgagcatcc
      721 gcgcgtggag acgttcgtgg ttcgcgtgtg gccgcacgag gaggccgatg ggctgcacgg
      781 tgtggtgcgg cacgtccgga gcggccggga ggtcaggttc tcgaacgcga cgcagctcgc
      841 cgacctcctg aggctgggat tccctcccga ggcccctgag ccgaccgggc cgcccgagga
      901 gccggccgca cgatgagcgg tccccctcag ccccgccgtc tccgcgggtg cgggtcgcgg
      961 gccggcgcgg gctcgtagcg gtcggtggga cgctcccggg cggcccgcga catcccgatc
     1021 agcgtgacgg accccaggac ccagtggccg atcgccagcg cccaccacag caccgcgtcc
     1081 gcaccccgag gcagcccgaa ggccgcgttc agggtgagca ccgcggccac ggccccggtc
     1141 gtcagcacga agccccacgc ccaccaccag agctcctcga gacggtgccc cacgaggacc
     1201 atgaagagcg ccaggccgac ggcctggacc cccacgagcc gcacccaggc gtagtccgga
     1261 tacctcggct gtcccagcag cgagaacagc agtccgggcc agatcgcgag gagcgtgccc
     1321 agcacgatcc aggcgagcgc gtgcgcgtag agggtcccgc ggagcagccg catgcctcca
     1381 tcttggaggg gaccgaccgg gatcgctacc ccactccggt cgtgaggggc ggagcccggt
     1441 acaatcactc gggagcgcgg ccgtgccgcg ctcgacgctt gccaaaccac cccgaaagga
     1501 cgctgaggca tgaccggacc gaggatcgcg gtgatcgggc tcgactgcgc cacgccgctg
     1561 ctgctgttcg atcggctgcg atcggagacc cccaacatcc aggccctcct cgggcggtcg
     1621 atgcacggcg agctggagag catcacgccc ccgatcacgg tgccggcctg ggcctgctcc
     1681 atgaccggga agacccccgg ccagctcggg atctacgggt tccgcaaccg caaggacacc
     1741 tcctacgacg gcctgtcgat cgccacctcg ctggcggtga aggagccggc ggtgtgggac
     1801 cagctgggcg aggccgggat gcgctccctg ctgatcggcg tcccgcccgc ctaccccatc
     1861 aagccggtcg aagggtggcg cgtctcgtgc ttcctcacgc ctccgtcggc cgatcagtac
     1921 acgcatcccg ccgacctcaa ggccgaggtc gaggaggtgg tgggggacta catcttcgac
     1981 atccccaact tccgtcagca ggggctcgac ttcgtgctct cccaatccca cgagatgacg
     2041 aagaagcgct tcgccctggc ccgccacatg gtgaggacca agccctggga cttcttcatg
     2101 atggtcgaga tggggaccga ccggctccac cacgtcttct ggcagtactg cgatccggac
     2161 cacccgaagt acgagccggg caacccctac
//
LOCUS       DASUTW010000091         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_27311, whole genome shotgun sequence.
ACCESSION   DASUTW010000091 DASUTW010000000
VERSION     DASUTW010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27311"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VF058_02905"
     CDS             complement(<1..336)
                     /locus_tag="VF058_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX6207287.1"
                     /translation="MSRLSGTLAASVTPLEDGGARPDEDAVGPIVDFLHEGGLDGILA
                     LGTTGEGIMLSGHERRRVAERFVEASAGRLRVAVHCGAQTTSETAALAAHAAEAGADA
                     VAVISPPYSP"
     gene            complement(333..>2211)
                     /gene="ftsH"
                     /locus_tag="VF058_02910"
     CDS             complement(333..>2211)
                     /gene="ftsH"
                     /locus_tag="VF058_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX6207288.1"
                     /translation="FVRTSDEISYSRFLSFVEQDRVEGLVRISGTSIEGTFRNQDGDQ
                     VQFRVIRPPDIQDEQLIGVLQENGVEFTGEQPSGFVSFLAVWGPVILIFGLMFLFFRR
                     MAGGGAGGALNLGKKGVKIYDRKDLKTTFDDVAGLEEAIEELREVVDFLANPKKSQRL
                     GGRIPQGVLLMGPPGCGKTLLARAVAGEAGVPFFYMSGSDFVEMFVGLGAARVRELFQ
                     QAKEKAPVLIFIDEIDTLGKSRSGAMGGNLGSHDEREQTLTQLLSEMDGFDTSKGVII
                     MAATNRPDVLDPALLRPGRFDRQVVVDRPDLKGREEILKVHAKEVVMDPEVDLHVLAA
                     RTPMLTGADLANIINEAALLAARREKEAVGMAELEEAIDRVTVGVERRSKVMSDRERE
                     LSAVHELGHALVALDLPGTNVLHRVTIIPRGRAGGVTYFRPTEDRTYHTDAELNDQLA
                     IALGGRVAEGIAFGDVSTGAHNDLANATQIARAMVIEYGMSEKVGPISFGNDGFRDAQ
                     GRPLFPGADRPNISDDTMRLIDEEVTRLLTQARERARAILEPKKDLMLRLAKVLMARE
                     TIEGSELKAWVDGDEPIPEPDEEPRAEEATRRLEPPEAAADGEAGGDGARETEEIHRP
                     T"
BASE COUNT          290 a          795 c          770 g          356 t
ORIGIN      
        1 gggggagtag ggcggcgaga tcaccgccac ggcgtcggcg ccggcctcgg ccgcgtgcgc
       61 cgccagcgca gccgtctcgc tcgtggtctg cgcgccgcag tggacggcga cccgcagccg
      121 tcccgcggag gcctccacga accgctcggc gacgcgccgc cgctcgtggc cggagagcat
      181 gatgccctcg ccggtcgtgc cgagcgcgag gatgccgtcc agccctccct cgtggaggaa
      241 gtcgacgatg ggtccgaccg cgtcctcgtc cggccgagcc ccgccgtcct ccagcggggt
      301 caccgaggcg gcgagggtgc cggagagcct gctcacgtgg gccggtggat ctcctcggtc
      361 tcccgcgccc cgtcgccccc ggcctcgccg tcggccgcgg cctccggagg ctcgagccgc
      421 cgtgtggcct cctcggcccg gggctcctcg tcgggctcgg ggatcggctc gtcaccgtcg
      481 acccatgcct tcagctccga gccctcgatc gtctcccggg ccatcaggac cttggccagc
      541 cgcagcatga ggtccttctt cggctcgagg atcgcgcggg cccgctcccg ggcctgggtg
      601 aggagccgcg tgacctcctc gtcgatcagc cgcatcgtgt cgtcggagat gttggggcgg
      661 tcggcgccgg ggaacagcgg gcggccctgc gcgtcgcgga agccgtcgtt cccgaaggag
      721 atcggcccca ccttctcgct catcccgtac tcgatgacca tggcccgggc gatctgggtg
      781 gcgttggcga ggtcgttgtg cgccccggtg gagacgtcgc cgaacgcgat cccctcggcg
      841 acccgtcctc cgagcgcgat ggccagctgg tcgttcagct cggcgtcggt gtgataggtg
      901 cggtcctccg tgggccggaa gtaggtgacg ccgcccgccc ggccccgagg gatgatcgtg
      961 acccggtgga ggacgttcgt ccccggcagg tcgagcgcga cgagcgcgtg accgagctcg
     1021 tggaccgcgc tcagctcgcg ctcccggtcg ctcatgacct tgctccggcg ctccaccccg
     1081 accgtgacgc ggtcgatcgc ctcctcgagc tcggccatgc cgacggcttc cttctcacga
     1141 cgggccgcca gcagggcggc ctcgttgatg atgttggcca ggtcggcgcc ggtgagcatc
     1201 ggcgtgcggg cggcgagcac gtggaggtcc acctcggggt ccatgacgac ctccttcgcg
     1261 tggaccttca ggatctcctc gcgtcccttg aggtcggggc ggtccacgac gacctggcgg
     1321 tcgaagcgcc ccggccgcag cagcgcgggg tcgagcacgt cggggcggtt cgtggcggcc
     1381 atgatgatca cgcccttgga cgtgtcgaag ccgtccatct cggagaggag ctgggtcagc
     1441 gtctgctcgc gctcgtcgtg ggagccgagg ttcccgccca tcgcgccgga gcggctcttc
     1501 cccagggtgt cgatctcgtc gatgaagatc agcaccgggg ccttctcctt cgcttgctgg
     1561 aacagctcac gcacgcgggc tgctccgagg cctacgaaca tctccacgaa gtctgacccc
     1621 gacatgtaga agaacgggac gcccgcctct ccggccacgg cccgcgcgag cagggtcttg
     1681 ccgcagcccg gcgggcccat caggagcacg ccctggggga tgcggccgcc gagtcgctgg
     1741 gacttcttgg ggttggcgag gaagtcgacg acctcgcgga gctcctcgat cgcctcctcg
     1801 agtccggcca cgtcatcgaa cgtggtcttg aggtccttgc ggtcgtagat cttcacgccc
     1861 ttcttcccga ggttgagcgc gcctccggcg cctcccccgg ccatgcgccg gaagaagagg
     1921 aacatcagcc cgaagatcag gatgaccggt ccccagaccg cgaggaagct cacgaacccg
     1981 ctcggttgct ctcccgtgaa ctccaccccg ttctcctgga gcacgccgat cagctgctcg
     2041 tcctggatgt cggggggacg gatcacgcgg aactggacct gatcgccgtc ctggttgcgg
     2101 aacgtcccct cgatcgaggt cccggagatc cgcacgagac cctcgacacg gtcctgctcc
     2161 acgaacgaga ggaagcggga gtaggagatc tcgtcgctcg tccgcacgaa g
//
LOCUS       DASUTW010000092         3346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1193385, whole genome shotgun sequence.
ACCESSION   DASUTW010000092 DASUTW010000000
VERSION     DASUTW010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193385"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VF058_02915"
     CDS             complement(<1..510)
                     /locus_tag="VF058_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX6207289.1"
                     /translation="MADLMSTITSMAKRRGIVFQSSEIYGGLRSFWDYGPLGVELKRN
                     VRAAWWKHMVQERDNVVGLESSIMMSPKVWEASGHLESFTDPLVECTSCHQRFRADHL
                     PGFHAPESGHAEGEEGTVEFEGDKPPACPNCGAREWTDPRNFNLMFKSFMGPVEADEN
                     VVYLRPETAQ"
     gene            599..1267
                     /locus_tag="VF058_02920"
     CDS             599..1267
                     /locus_tag="VF058_02920"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX6207290.1"
                     /translation="MEDGTTTLHGPGCIQEAMRMAGNARGPSAGLPRRAWAIAALLTL
                     LSCGGAEEATPREPSPTAAAASPTESPAITVADVLSTDERFTTLSQALVKDPLYLEVM
                     SRTGWNHTVFAPTDEAFGQLPAGRLASLLEDEERIPQLLDNHIVPELVPTDEMEDGDL
                     QTIRGVHEVDVGGDPIMIDGARIVEADLVTSNGIIHVIDGVFTVLCLQIGSGDPQCSD
                     LLEG"
     gene            1311..1892
                     /locus_tag="VF058_02925"
     CDS             1311..1892
                     /locus_tag="VF058_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX6207291.1"
                     /translation="MRSSTAALLIVAASLAACGGESETVTPTSPSSPSPTGPTPTPTE
                     IPGPDDGVLAILDDDGRFTIFLRILLVEGPERVAGFFTSPVWNNTLFAPTDEAFETLP
                     EGALEYLFGDDPQAEFDLLRVIEHHVVDQVRSIDDFEPGEVTSIAGPLRITVEGDRVM
                     IDGATVIEPDVEASNGIIHVIDGVLIPEQVDLG"
     gene            1961..2329
                     /locus_tag="VF058_02930"
     CDS             1961..2329
                     /locus_tag="VF058_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX6207292.1"
                     /translation="MKDLMVELEDRPGTLAELGEALGAAGVNISGIAGFRAGSAGVVH
                     VLVEDGDAAKGALEGAGANVVTERDVLMLDLENRPGSVGEVARKLADADINIDLIYVA
                     SDNRIVLSTEDLDAARRAIG"
     gene            2365..2688
                     /locus_tag="VF058_02935"
     CDS             2365..2688
                     /locus_tag="VF058_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018511719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX6207293.1"
                     /translation="MERRLRVRRAELEAELARLTEPPEAGSAVSFGKRIGDGTTEAVE
                     RIATTAQARSFSASMAEIDRALEKLSEGTYGRCDRCGEPIPKERLEVKPAATLCVRCS
                     EGASA"
     gene            2699..>3346
                     /locus_tag="VF058_02940"
     CDS             2699..>3346
                     /locus_tag="VF058_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6207294.1"
                     /translation="MTERTEYAPGAFCWVDLSTDDLEGAARFYGQLLGWQAEIEPGAG
                     GGSYAVFLIAGRRAAGGFHGKRIEWLSYVSVEDVDASAARADDLGADVIAGPRDVDGL
                     GRVAYLADPMGARFGVWQPGSTHGAEVVNVPGALTWNDLVCEGLETAQGFYGELFGWT
                     FQDAPGDPVAYSVILNGERSNGGIMVVPETPAHWRPYFVVGGADAAAARIGELGGR"
BASE COUNT          523 a         1098 c         1246 g          479 t
ORIGIN      
        1 ctgcgccgtc tcggggcgga ggtacacgac gttctcgtcg gcctccaccg gccccatgaa
       61 cgacttgaac atcaggttga agttgcgggg gtcggtccac tcgcgtgcgc cgcagttggg
      121 gcaggcgggg ggcttgtcgc cctcgaactc cacggtcccc tcctcgccct cggcgtggcc
      181 cgactcgggg gcgtggaacc cggggaggtg gtcggcgcgg aaccgctggt ggcagctcgt
      241 gcactcgacg agagggtcgg tgaacgactc gaggtgcccg ctggcctccc acaccttcgg
      301 cgacatcatg atcgaggact cgagccctac gacgttgtcg cgctcctgga ccatgtgctt
      361 ccaccacgcg gcgcggacgt tgcgcttcag ctcgaccccc aggggtccgt agtcccagaa
      421 cgagcggagc ccgccgtaga tctccgagga ctggaagacg atcccacggc gcttggccat
      481 ggaggtgatg gtgctcatga ggtcggccat cggggcctcc tttcccggtc gctggctgcg
      541 gccgctggcg tgggggtcag gatagcggac cgaggcgttg cgccggtctc cgcgagcgat
      601 ggaagatggc accaccacgc tgcacgggcc cgggtgcatc caggaggcga tgcggatggc
      661 gggaaacgcc cgagggccct cagcgggcct cccgcggagg gcgtgggcca tcgcggccct
      721 gctgaccctc ctctcgtgcg gaggggccga ggaggcgacc ccccgggagc cgtctcccac
      781 cgcagccgct gcctccccca ccgagtcccc cgcgatcacg gtcgcggacg tgctttcgac
      841 agacgagcgg ttcacgacgc tgtcacaagc cctcgtcaag gacccgctct acctggaggt
      901 catgagcagg accggctgga accacaccgt gttcgccccg accgacgaag cgttcgggca
      961 actgcccgcc ggtcgactgg cctcgctcct cgaggacgag gaacggatcc cacagctgct
     1021 cgacaaccac atcgtcccgg agctcgtgcc tacagacgag atggaagatg gggacctgca
     1081 gacgatccgt ggcgtgcacg aggtggacgt cggcggcgat ccgataatga tcgacggcgc
     1141 gcgcatcgtc gaggcggacc tagtgacctc gaacgggatc atccacgtga tcgacggcgt
     1201 cttcaccgtg ctctgcctgc agatcggcag cggcgatccg cagtgcagcg acctcctgga
     1261 gggatgagat ggttcgctcg cgcgcccggc ctcgctccct gaagagctcc ttgcggagtt
     1321 cgaccgcggc cctgctgatc gtcgccgcat ctctcgcagc gtgcggcggt gagagcgaga
     1381 cggtcacgcc gacgagcccc tcgagtccca gcccgacggg accgacgccc acgccgaccg
     1441 agatccccgg accggatgac ggcgtcctcg cgatcctcga cgacgacggg cggttcacca
     1501 tcttcctacg gatcctcctc gtggagggcc cggagcgggt cgccgggttc ttcacctcac
     1561 cggtgtggaa caacacgctc ttcgcgccga ccgacgaagc cttcgagacg cttcccgaag
     1621 gcgccctgga gtatctgttc ggcgacgacc cgcaggcgga gttcgatctc ctccgcgtga
     1681 tcgagcacca cgtggtggac caggtgagat cgatcgacga cttcgagccg ggcgaggtga
     1741 cgtcgatcgc cgggccgctc cggatcaccg tcgagggcga ccgggtcatg atcgacggcg
     1801 ccacggtgat cgagcccgac gtcgaggcct cgaacgggat catccacgtg atcgacgggg
     1861 tcctcatccc ggagcaggtg gacctcgggt agctccggca cgagttgtcg agagcgtcga
     1921 ggccgacgag actggtcgga agcggacgaa ggaggcgtgg atgaaggacc tcatggtgga
     1981 gttggaggat cggcccggga cgctggcgga gcttggggag gcgctgggcg cggcgggagt
     2041 gaacatcagc gggatcgcgg ggttccgggc gggcagcgct ggggtcgttc acgtgctcgt
     2101 cgaggacggc gacgcggcaa aaggggcgtt ggagggcgcg ggggcgaacg tcgtcaccga
     2161 gcgggacgtc ctgatgctcg atctggagaa ccgtcccggg agcgtgggcg aggtcgcgcg
     2221 gaagctcgcg gacgccgaca tcaacatcga cctgatctac gtcgcgtccg acaaccgcat
     2281 cgtgctctcc accgaggacc tcgacgcggc ccgtcgggcg atcggatagg gcctgcgcca
     2341 ctcggagcga tgaaggccga agcggtggag cgacgactcc gcgtccggcg cgcggagctc
     2401 gaggccgagc tagcgcgcct gaccgagccg cccgaggcgg ggtccgccgt gtcgttcggg
     2461 aagcggatcg gggacgggac cacggaggcc gtggagcgga tcgcgacgac ggcgcaggcg
     2521 cggtcgttct cggcctcgat ggccgagatc gaccgcgccc tggagaagct ctccgaaggc
     2581 acctacgggc gatgcgaccg ctgcggggag ccgatcccga aggagcgcct cgaggtgaag
     2641 ccggccgcga ccctctgcgt ccggtgcagc gagggagcct cggcgtgagg agggacccat
     2701 gacggagcgg acggagtacg cgcccggcgc gttctgctgg gtggacctca gcaccgacga
     2761 cctcgaggga gccgcgaggt tctacggcca gctgctgggc tggcaggcag agatcgagcc
     2821 cggggccggc ggcggctcgt acgccgtctt cctgatcgcc ggccgccgcg ccgcgggcgg
     2881 cttccacggg aagcggatcg agtggctctc gtacgtctcg gtcgaggacg tggacgcgtc
     2941 ggcggctcgg gcggacgacc tgggcgcgga cgtcatcgcg ggcccccgcg acgtggacgg
     3001 actcgggagg gtggcgtacc tcgccgaccc gatgggggcg aggttcgggg tgtggcagcc
     3061 ggggagcacc cacggggccg aagtcgtgaa cgtgccgggg gccctgacgt ggaacgacct
     3121 cgtctgcgag ggcctcgaga cggcccaggg cttctacggc gagctgtttg gatggacgtt
     3181 ccaggacgcc cccggggacc ccgtggccta cagcgtcatc ctcaacggcg aaaggagcaa
     3241 cggcggcatc atggtcgtgc ccgagacgcc ggcccactgg cgtccctact tcgtggtggg
     3301 cggcgcagat gcggcggccg cccggatcgg agagctgggc ggcagg
//
LOCUS       DASUTW010000093         5710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1582453, whole genome shotgun sequence.
ACCESSION   DASUTW010000093 DASUTW010000000
VERSION     DASUTW010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582453"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VF058_02945"
     CDS             <1..712
                     /locus_tag="VF058_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX6207295.1"
                     /translation="NINVGCGATHPETVAEAVRRFKADAGVAHDGDADRAILCDAEGS
                     IVDGDHVLGACALAMRDEGTLARNTVVTTVMANLGFRRAMEEAGISVVETQVGDRYVL
                     EEMRRTGATLGGEQSGHVIFLDAATTGDGLLTAARFLALARSKGVPVAELASVVRKLP
                     QVLVAVRVADREGLEGSEEVRRAIGSVEEALGGRGRVLVRPSGTEPLVRVMVEAEDEG
                     EARRHAEAIATAVREGLA"
     gene            780..2603
                     /gene="glmS"
                     /locus_tag="VF058_02950"
     CDS             780..2603
                     /gene="glmS"
                     /locus_tag="VF058_02950"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX6207296.1"
                     /translation="MCGIVGYVGRGEALPILMDGLRRLEYRGYDSAGVAVLGDDLRVV
                     KRAGKLDELEKALAEEGQPPGRIGMGHTRWATHGPPTDRNAHPQTDCSGAIAVIHNGI
                     IENFFEHRERLEAEGHEFRSETDTEVVAHLLEERYQGDLPAAVRSTVKELEGAYALVV
                     MAADRPDLVVGVRVSSPMIVGVGEGENLLASGAPAFLARTRLQAPLEDGEMVELRADG
                     YRITDLEGNERSAEPEEIDWDIEQAEKGGFEDFMLKEIHEQPQAILDTARGRIREGKL
                     LLDEVRMSEEDIRAVDKVFVVACGTAFHAGLVAKYAIEHWTRLPVEIEIASEFRYRDP
                     VLDRNTLTLAISQSGETIDTLEAVRHAKHQHSWVTAITNTVGSSVAREADAVVYTRAG
                     PEIGVAATKTFTTQMVALHLVALYLAQVRGTMYPEEIQQVLDELEGLPALVERTLELN
                     PGVEELARRYADARDVLFVGRHTGYPVALEGALKLKEISYIHAEGYPAGELKHGPIAL
                     LEPGVPVVAVATACHVYPKMLSNIQEIKARGAEVIALATEGDEEIGKLADHVLYVPRT
                     AELLSPVVVSVPLQLLAYHIAKIRGCDVDQPRNLAKSVTVE"
     gene            2637..3014
                     /locus_tag="VF058_02955"
     CDS             2637..3014
                     /locus_tag="VF058_02955"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF000832.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX6207297.1"
                     /translation="MRIVGIGVDIVDVTRVERMLDRYPRFPERVFTPTEVAYCESRAN
                     RATSYAGRWAAREATRKALGGIRRMRWHDVRVERAPSGAPRIVLEGTAKERADALGVS
                     EVLISFTHERTMATAFAIAVGEG"
     gene            3014..4552
                     /locus_tag="VF058_02960"
     CDS             3014..4552
                     /locus_tag="VF058_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX6207298.1"
                     /translation="MRPVLQAAQMGAADRGAEERGVPVEALMERAGHAVARAAAALLG
                     APYGRRVAAVCGKGNNAGDAFVAARLLAAWGAAPAVVAVAGLDELREAAAVNAGRLRD
                     VGVPVLTWGTREADRAIARADVLVDGLFGSGFRGLAEGPWAAAIEAMNASPAPVVAAD
                     IPSGVEGDTGAVRGPAVRAEVTVTFAAPKVGVVLYPGAELAGRVEVADIGIPADLLES
                     DLALVEPGDVAAILPRRAPDEHKHRSGDVLVVAGSRRMTGAPRLVAEGAARAGAGLVH
                     VAVPEGIFPIVQAGLTEAVFLPLPEGPEGSVAASAWGELEKRMDRFDAVAIGPGLTTE
                     GEAPDLVRALVRSSPVPIVADADALNAFADGSESLKDRSSELVITPHAGEFGRLFRVP
                     AADLAEDRVGLVRKAAADTGAVTVLKGPRTLVALPGGEVRINPTGSAALATGGTGDVL
                     AGAIAAYLARGLPPVEAATAAVYVHGLAGQVVGRRMGEGGTAEDVAIAIPAAVEAVLG
                     SVAS"
     gene            4569..5675
                     /gene="alr"
                     /locus_tag="VF058_02965"
     CDS             4569..5675
                     /gene="alr"
                     /locus_tag="VF058_02965"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX6207299.1"
                     /translation="MRYRPTVAVIDLDAVRHNVRVLSREGSAYMAVVKANGYGHGAVP
                     IARAALDAGAEWLGVALVEEGIALRDAGITAPVLVLTEFPRGSEKEALAADLTPVLYT
                     EEGIEGLAEAAETLGRRARVHVKVDTGMHRVGLLPDLAVGFLERAAERGLDIEGILTH
                     FAKSEAPDDPATRQQLATFGKLLTTLSARGSRPPICHAANTAASMVIPESRLDLIRIG
                     IGTYGIAPDPSLAGFADLRPVMSFRSRVSLVKRVERGHAVSYGFTYRLSRDATIATVP
                     VGYADGFARSLSNRARVLIRGQRYPVVGTVTMDQLLVDCGDDPVEAGDEVVLWGRQDD
                     EEILAEDVAAWWGTTGYEVVCAVSERVPREYLHG"
BASE COUNT          767 a         1949 c         2241 g          753 t
ORIGIN      
        1 gaacatcaac gtggggtgcg gggccaccca tcccgagacc gtggccgagg ccgtgcgccg
       61 gttcaaggcc gatgccggcg tggcgcacga cggcgacgcg gaccgggcca tcctctgcga
      121 cgcggagggg tcgatcgtgg acggcgacca cgtgctcggg gcgtgcgcgc tcgccatgcg
      181 cgacgagggg acgctggccc gcaacacggt cgtcaccacc gtgatggcca acctcggctt
      241 caggagggcg atggaggagg ccgggatctc cgtcgttgag acgcaggtcg gagaccggta
      301 cgtcctcgag gagatgcggc gcacgggggc cactctaggg ggggagcagt ccggccacgt
      361 gatcttcctc gacgcggcga ccaccggcga cggcctgctg actgcggcac gcttcctggc
      421 cctggcccgc tccaagggcg tgcccgtggc cgagctggcc tcggtcgtgc gcaagctgcc
      481 ccaggtcctc gtggcggtga gggtggccga tcgagagggc ctggaggggt cggaggaggt
      541 ccggcgcgcg atcggcagcg tcgaggaggc gctagggggt cgaggccggg tgctggttcg
      601 tccctcgggg acggagccgc tggtgcgcgt gatggtggag gccgaggacg agggcgaggc
      661 gcggcgtcac gcggaggcga tcgccaccgc cgtgcgcgag ggcctggcct gacggccggt
      721 tcgcccggct cgtcccggct ggtccgccga cccgaccccc cctggtacca tggggcgtca
      781 tgtgcggcat cgtgggctac gtgggtcggg gcgaggcgct cccgatcctc atggacgggc
      841 tccgccgcct cgagtaccga ggctacgact ccgcgggcgt cgcggtgctg ggcgacgacc
      901 tccgcgtcgt gaagcgagcc gggaagctgg acgagctcga gaaggcgctg gccgaggagg
      961 ggcagccgcc cggccggatc gggatgggac acacgcggtg ggccacccac ggccctccca
     1021 ccgaccggaa cgcccacccc cagacggact gctcgggcgc gatcgcggtc atccacaacg
     1081 gcatcatcga gaacttcttc gagcatcggg agcggctgga ggccgagggc cacgagttcc
     1141 gctccgagac cgacaccgag gtcgtggccc acctcctcga ggagcgctac cagggcgacc
     1201 ttcccgccgc cgtccgttcc accgtgaagg agcttgaggg cgcgtacgcc ctcgtggtga
     1261 tggccgccga ccggcccgac ctcgtcgtcg gggtgcgggt ctcctcgccg atgatcgtgg
     1321 gggtgggcga gggagagaac ctcctggcct cgggggcgcc cgccttcctg gccaggacca
     1381 ggctccaggc gccgctggag gacggcgaga tggtggagct gcgggccgac gggtaccgca
     1441 tcaccgacct cgaggggaac gagcgatcgg cggagccgga ggagatcgac tgggacatcg
     1501 agcaggcgga gaagggcggc ttcgaggact tcatgctcaa ggagatccac gagcagcccc
     1561 aggcgatcct cgatacggcc cggggccgga tccgcgaggg gaagctcctc ctcgacgagg
     1621 tccggatgag cgaggaggac atccgggcgg tggacaaggt gttcgtggtg gcctgcggga
     1681 ccgccttcca cgcgggactg gtcgccaagt acgcgatcga gcactggacc cgcctgccgg
     1741 tcgagatcga gatcgccagc gagttccgct accgggaccc cgtgctcgat cggaacacgc
     1801 tgacgctcgc catcagccag tccggggaga cgatcgacac gctcgaggcg gtccggcacg
     1861 cgaagcacca gcactcctgg gtgaccgcga tcacgaacac cgtggggtcc tcggtggccc
     1921 gcgaggcgga cgccgtcgtc tacacgcggg ccggccccga gatcggcgtc gccgccacga
     1981 agaccttcac cacgcagatg gtggccctcc atctcgtcgc gctctacctg gcgcaggtcc
     2041 gggggacgat gtaccccgag gagatccagc aggtgctgga cgagctggag ggcctcccgg
     2101 ccctcgtgga gcggacgctc gagctcaacc cgggggtgga ggagctggcc cgccggtacg
     2161 cggacgcgcg cgacgtgctc ttcgtggggc gccacacggg ctacccggtg gcgctggagg
     2221 gcgccctgaa gctgaaggag atctcctaca tccacgccga gggctacccg gccggggagc
     2281 tgaagcacgg gccgatcgcc ctgctcgagc ccggggttcc cgtggtggcg gtggccaccg
     2341 cgtgccacgt ctatcccaag atgctctcga acatccagga gatcaaggcc cgcggcgccg
     2401 aggtgatcgc cctggccacc gagggggacg aggagatcgg gaagctggcc gatcacgtcc
     2461 tctacgtgcc tcgaaccgcg gagctcctct cgccggtcgt ggtgtcggtg ccgctccagc
     2521 tcctcgcgta ccacatcgcc aagatccggg gctgcgacgt ggaccagccg cgcaacctgg
     2581 cgaagtccgt caccgtggag tgagggcgcg gggcgcgccg tgaggcggag cgcgtgatgc
     2641 ggatcgtcgg gatcggcgtc gacatcgttg acgtcacgcg cgtggagcgg atgctcgacc
     2701 ggtacccgcg gttccccgag cgcgtcttca cgcccaccga ggtggcctac tgcgagtcga
     2761 gggccaaccg agcgacgtcg tacgcgggcc ggtgggcggc ccgcgaggcg acgcgcaagg
     2821 cgctgggggg gatccggcgg atgcggtggc acgacgtcag ggtggagcgc gcgccgagcg
     2881 gggcgcccag gatcgtgctc gaggggacgg cgaaggagcg ggccgacgcc ctcggggtct
     2941 ccgaggtcct gatctcgttc acgcatgagc ggaccatggc caccgcgttc gccatcgcgg
     3001 tgggggaggg ctgatgcgcc cggtcctcca ggcggcgcag atgggagccg cggatcgggg
     3061 cgccgaggag cgcggcgtcc cggtggaggc gctgatggag cgggccgggc acgcggtggc
     3121 ccgggccgcc gccgcgctcc tcggcgcccc ctacggccgc cgcgtcgcgg cggtctgtgg
     3181 gaagggcaac aacgccggcg acgccttcgt ggccgcccgg ctcctggcgg cgtggggcgc
     3241 ggccccggcc gtcgtggcgg tggccggcct cgacgagctc cgggaggcgg cggccgtgaa
     3301 cgcggggagg ctgagggatg tcggcgtccc cgtgctcacg tgggggactc gggaagccga
     3361 tcgcgcgatc gcgcgggccg acgtgctggt ggacgggctg ttcggctccg ggttccgggg
     3421 gctcgcggag gggccctggg cggcggcgat cgaggcgatg aacgcctccc cggcccccgt
     3481 cgtggccgcg gacatcccgt cgggcgtgga gggggacacc ggcgcggtgc gggggccggc
     3541 cgtgcgggcc gaggtgacgg tgaccttcgc cgcgccgaag gtcggggtgg tcctctaccc
     3601 gggggccgag ctcgccgggc gggtcgaggt ggccgatatc ggcatcccgg ccgacctgct
     3661 ggagagcgac ctggccctgg tggagccggg tgacgtggcc gcgatccttc ctcgccgggc
     3721 ccccgacgag cacaagcacc ggtcggggga cgtcctcgtg gtcgccggct cgcgccgcat
     3781 gacgggagcc ccccgcctcg tggccgaggg tgcggcgcga gccggcgccg gcctcgtcca
     3841 cgtcgcggtg cccgagggca tcttcccgat cgtgcaggca gggctcacgg aggcggtctt
     3901 cctgcctctg cccgagggac cggagggttc ggtcgcagca tccgcctggg gggagctgga
     3961 gaagcggatg gaccgattcg acgcggtggc gatcgggccg gggctgacga ccgaggggga
     4021 ggccccggac ctcgtccgcg cgctcgtgcg ctcgtcaccc gtcccgatcg tggcggacgc
     4081 cgacgccctc aacgcgttcg ccgacggctc cgagagcttg aaggaccggt cttcggagct
     4141 ggtgatcacg ccgcatgcgg gcgagttcgg ccggctgttc cgcgtacctg ccgccgacct
     4201 cgctgaggat cgcgtcggcc tggtccgcaa ggccgcggcc gatacgggcg ccgtcacggt
     4261 cctcaagggc ccccggacgc tcgtggccct ccccgggggg gaggtgcgga tcaacccgac
     4321 cgggtcggcg gccctggcca ccggcgggac cggcgacgtg ctggccggcg cgatcgccgc
     4381 gtacctggcc cggggcctcc cccccgtcga ggccgccacc gccgccgtct acgtgcacgg
     4441 gctcgccggc caggtggtgg gccgccgcat gggcgagggg ggcacggccg aggacgtcgc
     4501 catcgcgatc cccgcggctg tcgaggccgt cctcggatcg gtggcatcat gacccggggg
     4561 gtgagcgcgt gaggtaccgg cccacggtcg cggtgatcga cctggacgcc gtccggcaca
     4621 acgtccgcgt cctgtccagg gagggctcag cgtacatggc cgtagtgaag gcgaacggct
     4681 acggccacgg cgcggtcccg atcgcccggg cggccctgga tgcgggggcc gagtggctcg
     4741 gcgtcgccct ggtcgaggag gggatcgcgc tccgggatgc cggcatcacg gccccggtgc
     4801 tcgtcctcac ggagttccca cgaggctccg agaaggaggc cctggcggcc gacctcaccc
     4861 cggtcctcta caccgaggag gggatcgagg ggctggccga ggcggccgag acgttgggtc
     4921 gccgggcgcg cgtgcacgtc aaggtcgaca ccgggatgca tcgggtgggg ctgctgcccg
     4981 acctcgcggt ggggttcctg gagcgggccg ccgagcgggg cctcgacatc gagggcatcc
     5041 tcacgcactt cgcgaagtcg gaggcccccg acgacccggc cacccgacag cagctcgcca
     5101 cgttcggcaa gcttctcacg acgctctctg cgcgcggctc gcgccctccc atctgccacg
     5161 cggcgaacac cgccgcctcg atggtcatcc ccgagtcccg gctggacctg atccgcatcg
     5221 gcatcgggac ctacggcatc gcgcccgatc cgtcgctcgc ggggttcgcc gatctgcgcc
     5281 ccgtgatgtc gttccgctcc cgcgtctcgc tcgtgaagcg ggtggaacga gggcacgcgg
     5341 tgtcgtacgg cttcacgtac cggctcagcc gggacgccac catcgccacg gtccccgtcg
     5401 ggtacgcgga cgggttcgcg cggtcgctct ccaaccgggc cagggtcctc atccggggcc
     5461 agcgctaccc ggtggtgggc acggtgacga tggaccagct gctcgtggac tgcggggacg
     5521 acccggtcga ggcgggggac gaggtcgtcc tgtggggccg gcaggacgac gaggagatcc
     5581 tcgccgagga cgtcgcggcg tggtggggga ccaccggata cgaggtcgtg tgcgccgtca
     5641 gcgagcgcgt cccgcgggag tacctccacg gatgaacacg gccaggaagg cggcggtcgg
     5701 ggcgctcgcg
//
LOCUS       DASUTW010000094         4852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1063846, whole genome shotgun sequence.
ACCESSION   DASUTW010000094 DASUTW010000000
VERSION     DASUTW010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063846"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..979)
                     /locus_tag="VF058_02970"
     CDS             complement(23..979)
                     /locus_tag="VF058_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6207300.1"
                     /translation="MSDRPGTVLVTGAAGFIGSHLSDRLLAQGYRVIGIDDLSVGRIA
                     NLAEARGYGKDFQFYNLDIRGEAIPAVFEKHQPELVMHLAAQSGVRPSLEDPRRDADI
                     NVLGTLNLVEAAARAGTRKLVYAASGGTIYGEPRRLPAKETAMSGSHPQSPYGISKRV
                     VHDYLLYFQRYEGLDFTSLALANVYGPRQDPHGEAGVVAIFGRRMLGGERPQINGDGN
                     QTRDYVYVEDVVDAFARAVDAGSGRLINIGTGLETSVNHLFRMLAELTGYAGEPSFGP
                     LPKGDQRRSSLDPSLAERDLGWKPWTQLEEGLRQTVEWLKAG"
     gene            complement(976..1980)
                     /gene="tsaD"
                     /locus_tag="VF058_02975"
     CDS             complement(976..1980)
                     /gene="tsaD"
                     /locus_tag="VF058_02975"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX6207301.1"
                     /translation="MKVFGIETSCDETAVAVVEDGVKIHSNLLASQHDVHARFGGVVP
                     ELASRAHVERLGPLMDRALDEAGIGWRDVEAVAVTRGPGLVGALLVGLATAKAAALAL
                     RVPFLGVNHLEGHVYANFLEHGPPEPPYVALIVSGGHTLLVHMPEEHRYEVVGQTLDD
                     AAGEAFDKIARFLGLGFPGGPALDNLAQEGDPEAIRFPRAMAGSGDYDFSLSGLKTAV
                     IRHVRREREAGREIPMADLAASFQEAIVDVQVQKTFAAAQERAVETVLLGGGVVANTR
                     LRARMSEEARRRGVRLHYPPPELCTDNAAMIASAGYFRIRRGERDGWDVAADPGLALA
                     "
     gene            complement(1977..2471)
                     /gene="rimI"
                     /locus_tag="VF058_02980"
     CDS             complement(1977..2471)
                     /gene="rimI"
                     /locus_tag="VF058_02980"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX6207302.1"
                     /translation="MRRRHLKGVLAIERQVYPRPWSSNLFLAELRNHKDRAYYVALID
                     REVVGYGGLLSYGDEAHVTTIAVHPDHQRHKIGTRLLIELIDQARRMDGVAVSLEVRV
                     TNHGAQRMYGRFGFRPVGVRKNYYQEIGEDALVMWTDDIRTEEYTRLLEGLRASLPEG
                     LRRP"
     gene            complement(2488..3192)
                     /gene="tsaB"
                     /locus_tag="VF058_02985"
     CDS             complement(2488..3192)
                     /gene="tsaB"
                     /locus_tag="VF058_02985"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX6207303.1"
                     /translation="MLVLALETSTPQTSVAIGSEQGVVASAVVSWGRGHSEVTVPAIE
                     RLLAWSEIDLDQVGGVAVGVGPGLFTGLRVGISTAKGLAQVLGLPMVGLGSLDVLAYS
                     VRYARERICAVLDAKRGEVFWSFYRTVPGGVTRETEPSVGPPERLAAELEASPDETLL
                     VGGGALMHRRVLERDASGIDFASTANAFPLAVWLLELAVPRFHREETSSPHEILPLYL
                     RKSDAEINWDRRARSA"
     gene            complement(3192..3683)
                     /gene="tsaE"
                     /locus_tag="VF058_02990"
     CDS             complement(3192..3683)
                     /gene="tsaE"
                     /locus_tag="VF058_02990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX6207304.1"
                     /translation="MGSEIRLRTRSADESREVGAAIARALIPGDVVSLTGDLGAGKTT
                     LVQGAARELGVTDPVASPTFVLVREYRGPLPIYHVDVYRLERIQEVLDLGFEDLLDPH
                     GVVFIEWGDAIEGLLPDSHLEVELHALEGDDEREVVVRARGPRWTVRWIDLSGSLGSW
                     RAA"
     gene            complement(3683..4279)
                     /locus_tag="VF058_02995"
     CDS             complement(3683..4279)
                     /locus_tag="VF058_02995"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX6207305.1"
                     /translation="MSQLEEIAQAASTCTRCGLSAGRTQVVFGDGNPNAGIMFIGEGP
                     GFHEDRQGVPFVGAAGQLLNRLLEEVGLSRGDVYIANVVKCRPPGNRDPMPDEIEACS
                     PYLMQQVDLVDPRVIATLGNFATRVILQRQVSISRVRGQKEVWRGRTVIPTFHPAAAL
                     HGGGEGGPQMRALREDFAAIKAALEEGAPAVEEQLGLF"
     gene            complement(4276..>4852)
                     /locus_tag="VF058_03000"
     CDS             complement(4276..>4852)
                     /locus_tag="VF058_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX6207306.1"
                     /translation="PPAVRPGPDQGYAACEAASEEIPEGSVGAGTGATVGKLRGREHA
                     VKGGVGTSSAAEDGVTVGALVAVNAVGAVVDEEGAPIAGRLLEEGEEPSPPFGNTTLA
                     VVATDARLSRERAHLLAIAAHDGLASAIRPAHTMWDGDTVFTLATGAVDAPQPLLERL
                     AEDALADAVRRAVRLARGLAGIPPAGEEGGR"
BASE COUNT          659 a         1844 c         1674 g          675 t
ORIGIN      
        1 gcgggtggcg ccccgcgccc cctcatcccg ccttgagcca ctccacggtc tggcggaggc
       61 cctcctcgag ctgggtccac ggcttccacc ccaggtcccg ctccgccagc gaggggtcca
      121 gcgacgaccg acgctggtcg cccttgggga gcgggccgaa ggagggctcg cccgcgtatc
      181 cggtgagctc cgccagcatc cggaacaggt ggttgacgct cgtctccagc ccggtcccga
      241 tgttgatcag ccgtcccgac cctgcgtcca cggccctggc gaaggcgtcc accacgtcct
      301 cgacgtagac gtagtctcgc gtctggttcc cgtccccgtt gatctggggg cgttccccgc
      361 ccaacatccg ccgcccgaag atcgccacga ccccggcctc cccgtgcggg tcctgccgcg
      421 gcccgtagac gttggccagg gccagcgagg tgaagtcgag gccctcgtac cgctggaagt
      481 acagcaggta gtcgtgcacg acccgcttgc tgatcccgta cggggactgg ggatggctgc
      541 cggacatcgc cgtctccttc gcgggcagcc tgcggggctc cccgtagatc gtcccccccg
      601 aggcggcgta gacgagcttc cgcgtccccg cacgggcggc ggcctccacg aggttcagcg
      661 tccccagcac gttgatgtcg gcgtccctac gcgggtcctc gagcgagggc cgcacgccgg
      721 actgcgcggc gaggtgcatc acgagctcgg gctgatgctt ctcgaacacg gcggggatgg
      781 cctccccccg gatgtcgagg ttgtagaact ggaagtcctt gccgtagccc cgggcctcgg
      841 ccaggttcgc gatgcggccg accgagaggt cgtcgatccc gatcacccgg tacccctgcg
      901 ccagcaggcg gtccgagagg tgggacccga tgaacccggc cgcccccgtg acgaggacgg
      961 tgcccggtcg gtcgctcatg ccagcgccag ccccgggtcg gcggccacgt cccaaccgtc
     1021 gcgctcgccc cggcggatcc ggaagtaccc ggccgaggcg atcatcgccg cgttgtccgt
     1081 gcagagctcc gggggcgggt agtggaggcg cacgccgcgg cggcgcgcct cctcgctcat
     1141 ccgggctcgg aggcgcgtgt tcgccaccac gcccccgccc agcagcacgg tctccacggc
     1201 ccgctcctgg gccgccgcga acgtcttctg cacctgcacg tccacgatgg cttcctggaa
     1261 cgaggcggcg aggtccgcca tcgggatctc gcgtccggcc tcccgctcgc gccggacgtg
     1321 gcggatcacc gccgtcttca ggcccgagag ggagaagtcg tagtcgcccg atcccgccat
     1381 ggcccggggg aagcggatcg cctcggggtc gccttcctgc gccaggttgt cgagcgccgg
     1441 gccgcccggg aagccgaggc cgaggaacct cgcgatcttg tcgaaggcct cccccgcggc
     1501 gtcatccagg gtctgcccca cgacctcgta ccggtgctcc tcgggcatgt ggacgagcag
     1561 cgtgtgcccg ccggagacga tcagggccac gtacggaggc tcgggtggcc cgtgctccag
     1621 gaagttcgcg tagacgtgcc cctcgaggtg gttcacgccc aggaagggga ccctcagggc
     1681 cagcgcggcg gccttggccg tggcgaggcc gacgaggagg gcccccacga ggcccgggcc
     1741 tcgcgtcacc gccacggcct cgacgtcccg ccacccgatc ccggcctcgt ccagggctcg
     1801 atccatcagc gggcccagcc gctcgacgtg ggcccgggag gcgagctccg gcacgacccc
     1861 gccgaaccgc gcgtggacgt cgtgctggct ggccaggagg ttcgagtgga tcttcacccc
     1921 gtcctccacg acggccaccg cggtctcgtc gcaggaggtc tcaatcccga agactttcat
     1981 ggcctgcgca gcccctcggg cagggacgcc cggagcccct cgaggaggcg cgtgtactcc
     2041 tcggtccgga tgtcgtcggt ccacatcacg agcgcgtcct cgccgatctc ctggtagtag
     2101 ttcttccgca cgcccacggg gcggaacccg aaccgcccgt acatccgctg cgccccgtgg
     2161 ttcgtcaccc gcacctcgag ggagacggcc accccgtcca tcctcctggc ctgatcgatg
     2221 agctcgatca ggagacgggt cccgatcttg tgccgctggt ggtcggggtg gacggcgatc
     2281 gtggtgacat gcgcctcgtc accgtaggag aggaggcctc cgtagccgac gacctcgcgg
     2341 tcgatcagcg ccacgtagta cgcccgatcc ttgtggttgc ggagctccgc gaggaacagg
     2401 ttcgagctcc acggccgagg gtacacctgc cgctcgatcg ccagcacccc cttgaggtgc
     2461 cggcgacgca tgcgcgtcac ctcgacctca ggcgctccgc gctcgtcgat cccagttgat
     2521 ctccgcatcc gacttcctca ggtagagggg gaggatctcg tgcgggctgg acgtctcctc
     2581 tcgatggaac cggggcaccg ccagctcgag gagccacacc gccagcggga aggcgttggc
     2641 cgtggaggcg aagtcgatcc ccgacgcgtc gcgctccagg accctgcggt gcatcagggc
     2701 cccgccaccc acgaggagcg tctcgtccgg cgaggcctcg agctccgcgg ccagccgctc
     2761 gggagggccg acggatggct cggtctcgcg ggtcacgccc cccggcacgg tccggtagaa
     2821 ggaccagaac acctctcctc gcttcgcgtc gaggacggcg cagatccgct ccctcgcgta
     2881 ccgcaccgag taggccagga cgtcgagcga ccccaggccc accatgggga gcccgaggac
     2941 ctgggccagc cctttggccg tggagatccc gacgcggagg ccggtgaaga ggcccgggcc
     3001 gaccccgacg gcgacgccgc ccacctggtc gaggtcgatc tcggaccagg ccagcaggcg
     3061 ctcgatcgcc gggacggtga cctcggagtg gccgcgcccc cacgagacca cggccgaggc
     3121 cacgacgccc tgctcgctcc cgatggccac gctcgtctgg ggggtcgagg tctccagcgc
     3181 cagtaccagc atcaggcggc cctccaggag ccgagcgagc cggagaggtc gatccagcgg
     3241 acggtccacc gcgggccccg agcgcgcacc acgacctcgc gctcatcgtc cccctcgagc
     3301 gcgtggagct cgacctcgag gtgggagtcc gggaggagcc cctcgatcgc gtctccccac
     3361 tcgatgaaga cgaccccgtg ggggtcgagc aggtcctcga agccgaggtc cagcacctcc
     3421 tggatccgct ccagccggta gacgtccacg tggtagatcg ggagcggccc acggtactcg
     3481 cggacgagca cgaaggtggg ggatgccacc gggtccgtca ccccgagctc ccgggccgcg
     3541 ccctgcacca gcgtcgtctt cccggccccg aggtcgccgg tgaggctcac cacgtcgccc
     3601 gggatcagcg cgcgggcgat cgccgcgccc acctcccgcg actcatccgc cgagcgggtc
     3661 cgaagccgga tctcgctccc catcagaaga gcccgagctg ctcctcgacg gcgggagccc
     3721 cctcctcgag ggccgccttg atcgcggcga agtcctcacg gagcgcccgc atctgggggc
     3781 cgccctcgcc gccgccgtgc agcgcggcgg ccgggtggaa cgtagggatc acggtgcggc
     3841 cgcgccacac ctccttctgt ccccggacgc gggagatcga cacttgccgc tgcaggatca
     3901 cccgcgtggc gaagttcccc agcgtcgcga tgacccgggg gtcgaccagg tcgacctgct
     3961 gcatgaggta gggcgagcag gcctcgatct cgtccggcat gggatcgcgg ttccccgggg
     4021 ggcggcactt caccacgttg gcgatgtaga cgtcccccct ggagagcccg acctcctcca
     4081 ggaggcgatt caggagctgt ccggcggccc cgacgaacgg gacgccctga cggtcctcgt
     4141 ggaagcccgg gccctccccg atgaacatga tcccggcgtt cgggttcccg tctccgaaca
     4201 cgacctgcgt ccgtcccgcc gacaggccgc acctggtgca ggtggatgcg gcctgggcga
     4261 tctcctcgag ctgactcatc ggcccccttc ctcgccggcc ggggggatgc cggcgaggcc
     4321 ccgggccagc cgcaccgccc gccgaacggc gtccgcgagc gcgtcctcgg cgaggcgctc
     4381 gaggagcggc tgaggtgcgt ccacggcgcc cgtcgccagc gtgaagacgg tgtctccgtc
     4441 ccacattgta tgcgccggcc ggatcgcgct cgcaaggccg tcgtgggcgg cgatcgccag
     4501 gaggtgggcc cgctcacgcg agagccgggc gtcggtggcc acgacggcca gggtggtgtt
     4561 cccgaacggc ggggagggct cctccccctc ctcgaggagc cgcccggcga tcggcgcgcc
     4621 ctcctcgtcc acgacggccc cgacggcgtt caccgcgacg agcgcgccca cggtcacccc
     4681 gtcctcggcg gccgacgagg tccccacgcc gcccttcacc gcgtgctccc ggccgcgcag
     4741 cttccccacg gtggcccccg tgccggcccc cacgctgccc tcggggatct cctccgaggc
     4801 ggcctcgcag gcggcgtacc cctggtccgg gcccggccgg acggcggggg gg
//
LOCUS       DASUTW010000095         6761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1064036, whole genome shotgun sequence.
ACCESSION   DASUTW010000095 DASUTW010000000
VERSION     DASUTW010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064036"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..1371
                     /locus_tag="VF058_03005"
     CDS             79..1371
                     /locus_tag="VF058_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207307.1"
                     /translation="MRGRTIVSASLVILLLAVAVPSLAAPKTRRMSVRSNGTDHVGDS
                     DVPDITPSGRYVVFASSAELIPSDMNTNVDIYLRDQRKKKTRLLSMRSDGSIEPSGNS
                     EHPSISASGRFVVFESSGELIPADGYPGSDIYLRDRKKRKTILVSRRSGGSQGDQGSY
                     TPAISADGRFVAFQTSSKLVGKDTNGAADIYVRNLVKKKTRLVSASASGQVGDGESFD
                     AAISADGRFVAFASNSSDLVPTDGNGAADIFVKDMKTRRIRRVSVRSNGAEGTDPSHN
                     PQISANGRWVVFASESLLAGQDVGAFRNVFVHDRKTRKTRLVSKSSAGATATCASPGS
                     ITGLDISPDGRWVAFDSCADNLGNPMGNYSVYLHDRVKGRTRLVSRRNGGGISPDTNE
                     QPAVSKGGLFVAWTSDDEMIVGGDSNTSDDVFRWGPRK"
     gene            1416..2816
                     /gene="glmU"
                     /locus_tag="VF058_03010"
     CDS             1416..2816
                     /gene="glmU"
                     /locus_tag="VF058_03010"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX6207308.1"
                     /translation="MAPKPSRRLAAVVMAAGKGKRLRSSLPKVLHPVAGKPVLWHVLR
                     AARRLRPWRMIVVVSHGRDHVEETVRSWGLDVTFVDQGEPLGTGHAVMAAEGAVGRAD
                     DVLVLPGDNPLVTTEMLRDLMRLHRRRLPAATVQTTVLDDATGYGRVIRDGDRFERIA
                     EERDASSTERRIHEIATSVYAFRREDLFGALPLVGRENVQGEHYLPDVLGILAGKGED
                     IRAIEADFEGALDVNSRVALARATAVMRRRINEELMASGVTMVDPDRTYVDAGVKIGA
                     DTTLLPGVHLEGDTRIGAGCTIGPDARIVDSRIDDEVEITSSTVRGARIGQGSTVGPY
                     AHLRPGTVMGRRTKAGSFVEIKASRIGDGSKVPHLAYVGDATIGKDVNVGAGTITCNY
                     DGYEKHRTLIGDGAFIGSDTMLVAPVRIGRNAVTGAGSTITRDVAEGELAVERSEQRN
                     VRGYTERRAKRRRGRT"
     gene            2813..3805
                     /locus_tag="VF058_03015"
     CDS             2813..3805
                     /locus_tag="VF058_03015"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEX6207309.1"
                     /translation="MSTIREIVTKKRMMIFAGTSHPELALEIATHLGVELAPAKLFRF
                     ASSEIYMRSTESARGSDAFVIQTHADPVNESIMEQLIMIDALKRASAKRITAVVPYYG
                     YARQDKKSLAREPISARLVADLISVAGADRVVSVDLHSGQIQGYFDFPFDHLTALPLL
                     SDFLKDDLGLGGEEMVVVAPDAGRVKTAERLREHLDSELAFIYKRRSRHEAHKIEEMV
                     VEGDVDGRACILIDDMIDTAGTMVEGAVALQREGASRIYAAATHPVFSGKATQRLEES
                     AIQKVVVTNTLPVPSEKQLEKLTVLSIAPIIASALQAVFEDESVSEIFHGQNMP"
     gene            complement(3808..5100)
                     /locus_tag="VF058_03020"
     CDS             complement(3808..5100)
                     /locus_tag="VF058_03020"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6207310.1"
                     /translation="MSDREDVPPIASRRARAVARARGIAVDLTPLRVSRDYRLLWLGE
                     MASHTGRHITVVALPFQIYQLTGSPLAIGMMGLAQVVPLVILSQIGGVIADRVDRRRL
                     LAITQVGLAAASLGLAIAASLGSPPLWFLYLVAAVIGGLSGIESPTRAATVPRLVGTT
                     HVPSAIALEQTLFQLSDVIGPAIGGLVLASLGLSWAYALDASSSVVAFGMVLAMRPLP
                     PDPSQRQVKGTTAFREGFRFLRGRRVLQAAFQVDLIAMVFGMPRALFPVLAVEVFHVG
                     PRGLGLLYAAPGAGALLGALTSGWVRHVRRQGQAVLLAVVVWGLAIAAFGLTDVLWLA
                     LVLLGIAGAADMLSAIFRATILQLSIPDRLRGRLSAIHIAVVVGGPRLGDMEAGVVAA
                     VVSPTFSVVSGGVATVIGVGVLAALVPAFVRYRSGDPA"
     gene            5186..5884
                     /locus_tag="VF058_03025"
     CDS             5186..5884
                     /locus_tag="VF058_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX6207311.1"
                     /translation="MEVKLAAEKREESGKGPARRARAAGRVPGVLYGPQLDPVNFTVD
                     LMDLWHALHTAAGVNVLVDLKVDGEEYLTMPREVQRDILKGTLLHVDFIQIDRNKPIQ
                     VHVPIVLTGEAEGQKAGGVVEHHLWELHVEVLPLEVPESVEADITELEMNDTLHVSDL
                     TVPEGATVLSSEEEPVVSIVPPPVIELPEPEVEEEELLEGEEGELVEGEEAGEAAEGE
                     GGEAPAEEGESEEG"
     gene            5885..6463
                     /gene="pth"
                     /locus_tag="VF058_03030"
     CDS             5885..6463
                     /gene="pth"
                     /locus_tag="VF058_03030"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX6207312.1"
                     /translation="MSAWLVVGLGNPGDRYAETRHNIGAWVVERLSDRLNARLRKVRF
                     MPLLVGEATVDGERVYLLRSQTFMNVTGPPVASFIRKRKLDLDRVVVVHDEIDLPFGS
                     LKVKQGGSTAGHNGLRSLVGAIGSPDFLRVRLGVGRPPGRQDPADHVLRPFAKAERED
                     AEILAEEAADAVLTLIRDGLSVAQDRHNRSVR"
     gene            6471..6611
                     /locus_tag="VF058_03035"
     CDS             6471..6611
                     /locus_tag="VF058_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207313.1"
                     /translation="MSDPKPRRRLIERAILGTFMGLAAFIIERRVRKALRRAEAERGG
                     DR"
     gene            6608..>6761
                     /locus_tag="VF058_03040"
     CDS             6608..>6761
                     /locus_tag="VF058_03040"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF013780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HEX6207314.1"
                     /translation="MSKRTVEKTDEEWRRELTPERYAVLRQQATEAPFTSELLQVKED
                     GTFRCAA"
BASE COUNT         1132 a         2356 c         2396 g          877 t
ORIGIN      
        1 cccccccgcc gcccccttgt cagcccgacg cgcagcgcgt agagtcccgg agccgtcgct
       61 cgtccaggga ggtcacccat gcgcggtcgc acgatcgtgt ccgcatcgct cgtcatcctg
      121 ctgctcgcgg tcgccgtccc ctcgctcgcc gcgccgaaga cccggcggat gagcgtccgt
      181 tcgaacggaa cggatcacgt cggggacagc gacgtccccg acatcacgcc ttcgggtcgc
      241 tacgtggtct tcgcctcgtc cgccgagctg atcccgtccg acatgaacac caacgtggac
      301 atctacctcc gcgaccagcg gaagaagaag acgcgcctgc tctcgatgcg gtccgatggc
      361 agcatcgagc cctccgggaa cagcgagcac ccgtcgatct cggccagcgg gaggttcgtg
      421 gtgttcgagt cgagcggcga gctgatcccc gccgatggct atcccgggtc cgacatctac
      481 ctccgggacc ggaagaagcg gaagacgatc ctggtgagcc gacgatcggg cggctcgcag
      541 ggtgaccagg ggagctacac gcccgccatc tcggccgacg gccgcttcgt cgccttccag
      601 accagctcga agctcgtggg gaaggacacc aacggcgccg ccgatatcta cgtgcggaac
      661 ctggtcaaga agaagacgag gctggtcagc gcgagcgcct ccgggcaggt cggggacggc
      721 gagagcttcg acgcggcgat ctcggccgac ggccgcttcg tcgccttcgc ctccaactcc
      781 tcggatctgg ttcccaccga cggcaacggc gccgccgaca tcttcgtgaa ggatatgaag
      841 acgaggagga tccgccgggt gagcgtgcgc tcgaacggag ccgaggggac tgacccgagc
      901 cacaacccgc agatctcggc gaacggccgc tgggtggtct tcgcctccga atcgctgctc
      961 gcggggcagg acgtcggcgc gttccgcaac gtcttcgtcc atgatcggaa gacgcggaag
     1021 acgcgcctcg tcagcaagag ctccgcgggg gccaccgcga cctgcgccag ccccggctcg
     1081 atcaccggcc tggacatctc gcccgacgga cgctgggtcg ccttcgactc gtgcgcggac
     1141 aacctgggca acccgatggg caactacagc gtctacctcc acgaccgcgt caagggcagg
     1201 acgcggctcg tcagccggag gaacgggggc gggatctcgc ccgatacgaa cgagcagccg
     1261 gccgtatcga agggcgggct cttcgtagcc tggacctccg acgacgagat gatcgtcgga
     1321 ggggactcga acacgtccga tgacgtcttc cgatgggggc ccaggaagta gccccgtcgg
     1381 atgctccccg ggccctcggc gagtacccta ccggcgtggc tccgaagccc tcccgtcgtc
     1441 tcgccgcggt cgtgatggcc gcgggcaagg ggaagcgcct ccggtcctcc ctgcccaagg
     1501 tcctgcatcc ggtcgcgggg aagcccgtgc tgtggcacgt cctccgggcg gcccgccgac
     1561 tccgtccctg gcgcatgatc gtggtggtca gccacgggcg ggatcacgtc gaggagacgg
     1621 tgcgctcgtg gggcctcgac gtgaccttcg tggatcaggg cgagccgctg ggcacgggcc
     1681 acgccgtgat ggcggccgag ggggcggtgg ggcgggccga cgacgtgctc gtcctccccg
     1741 gcgacaaccc gctcgtcacc accgagatgc tgcgagacct gatgaggctc caccgtcgac
     1801 gcctccccgc cgccaccgtg cagacgacgg tgctcgacga cgccaccggg tacgggcggg
     1861 tgatccggga cggcgatcgc ttcgagcgga tcgccgagga gcgcgacgcc tcgtcgaccg
     1921 agcgccgcat ccacgagatc gccacgagcg tctacgcctt ccgccgggag gacctcttcg
     1981 gcgccctccc cctggtgggc cgggagaacg tccagggcga gcactacctc cccgacgtcc
     2041 tgggtatcct cgcgggcaag ggcgaggaca tccgggccat cgaggccgac ttcgagggcg
     2101 ccctcgacgt gaactcgcgg gtggcgctgg cgcgcgccac cgccgtgatg cgccgccgca
     2161 tcaatgagga gctgatggcc tcgggcgtga cgatggtcga ccctgaccgc acgtacgtgg
     2221 atgcgggggt gaagatcggg gccgacacca ccctgcttcc gggagtgcac ctcgagggcg
     2281 acaccaggat cggcgcgggc tgcacgatcg gtcccgacgc ccggatcgtg gactcgagga
     2341 tcgacgacga ggtcgagatc acctcctcca ccgtccgggg cgcgaggatc ggccagggct
     2401 cgacggtcgg gccctacgcc cacctcaggc cggggaccgt gatgggccgc cgcacgaagg
     2461 cggggtcctt cgtcgagatc aaggcctcga ggatcgggga cggctcgaag gtcccccacc
     2521 tcgcctacgt tggcgacgcc acgatcggga aggacgtcaa cgtgggcgca gggacgatca
     2581 cctgcaacta cgacggatac gagaaacacc ggaccctgat cggggacggg gccttcatcg
     2641 gttccgatac gatgttggtc gcaccggtga ggatcggccg gaacgcggtc acgggcgcgg
     2701 ggtcgacgat cacgcgcgac gtggccgagg gggagctggc ggtggagcga tccgagcagc
     2761 ggaacgtccg ggggtacacg gagcggcgcg cgaagcgccg gagggggagg acgtgagcac
     2821 catccgggag atcgtcacga agaagcggat gatgatcttc gccgggacct cccatcccga
     2881 gctggcgctg gagatcgcca cgcatctcgg ggtggagctg gcgccggcga agctcttcag
     2941 gttcgccagc agcgagatct acatgcggtc cacggagagc gcgcggggct ccgacgcgtt
     3001 cgtgatccag acgcacgccg acccggtgaa cgagtcgatc atggagcaac tcatcatgat
     3061 cgacgcgctc aagcgggcct ccgccaagcg catcaccgcg gtggtcccgt actacggcta
     3121 cgcgcggcag gacaagaagt cgctggcccg cgagccgatc tcggcccggc tcgtggccga
     3181 cctcatctcg gtggccgggg ccgaccgcgt ggtctcggtg gacctccatt cgggccagat
     3241 ccagggctac ttcgacttcc ccttcgatca cctgacggcg ctcccgctgc tctcggactt
     3301 cctcaaggac gacctcgggc tcggaggcga ggagatggtg gtcgtcgcgc cggacgcagg
     3361 ccgcgtcaag acggccgagc gtctccgcga gcacctcgac tccgagctgg ccttcatcta
     3421 caagcgccgc tcccggcacg aggcgcacaa gatcgaggag atggtggtcg agggcgacgt
     3481 ggacggacgg gcctgcatcc tgatcgacga catgatcgac acggcgggga cgatggtcga
     3541 gggggcggtg gccctgcagc gcgagggcgc ctcgcggatc tacgccgcgg ccacgcaccc
     3601 cgtcttcagc gggaaggcga cccagcgcct cgaggagtcg gcgatccaga aggtggtggt
     3661 gacgaacacg ctgccggtgc cctccgagaa gcagctcgag aagctcaccg tgctctcgat
     3721 cgcgccgatc atcgcgtcgg cgctccaggc cgtgttcgag gacgagtcgg tctccgagat
     3781 ctttcacggc cagaacatgc cgtagcctca ggccgggtcg cccgagcggt agcggacgaa
     3841 ggcggggacg agggcggcca gcacaccgac accgatcacg gtggcgacgc ctccggagac
     3901 caccgagaac gtgggcgaga ccacggcggc caccacgccc gcctccatgt ccccgagccg
     3961 ggggcctccc accaccacgg cgatgtggat ggccgacagc ctcccccgga gacgatcggg
     4021 gatcgagagc tggagaatcg tggcgcgaaa gatcgcgctc agcatgtcgg cggcgccggc
     4081 gatccccagc agcacgagcg ccagccacag aacgtcggtc agcccgaacg ccgcgatcgc
     4141 cagcccccac accacgaccg cgaggagcac cgcctgtccc tgtcggcgca cgtgccgcac
     4201 ccagccgctc gtgagcgcgc ccagcagggc gccggcgccg ggcgccgcgt acaggagccc
     4261 cagcccccgg ggcccgacgt ggaagacctc gacggccagc accgggaaca gcgcccgagg
     4321 catcccgaag accatcgcga tcaggtccac ctggaacgcg gcctgcagca cgcgccgccc
     4381 gcgcaggaac cggaacccct cgcggaacgc cgtcgtcccc ttcacttggc gttgagaagg
     4441 gtccgggggc agcggccgca tcgccagcac catcccgaac gccacgaccg aggaggacgc
     4501 gtcgagcgcg taggcccagg acaaccccag gcttgccagc acgagccccc cgatcgccgg
     4561 gccgatcacg tcgctgagct ggaagagggt ctgctccagc gcgatcgccg aggggacgtg
     4621 cgtcgtcccc acgagccgag gcaccgtggc cgccctcgtc ggggactcga tccccgacag
     4681 ccccccgatg accgcggcca cgaggtacag gaaccagagc gggggcgacc cgagggacgc
     4741 cgcgatcgcc aggccgagcg acgccgccgc cagccccacc tgggtgatcg cgaggagccg
     4801 ccgccggtcc acccggtccg cgatcacccc tccgatctgg gagaggatca cgagcggcac
     4861 gacctgggcc agccccatca tcccgatggc gagcggcgat cccgtgagct ggtagatctg
     4921 gaaggggagg gcgacgaccg tgatgtgccg gcccgtgtgc gaggccatct cccccagcca
     4981 cagcagccgg tagtcgcgcg acacccggag gggcgtgagg tccacggcga tcccgcgcgc
     5041 cctggccacg gcccgggcgc ggcgcgatgc gatgggcggg acgtcctccc gatccgacat
     5101 agcgaaggga tgctagcggg tcggtgaggg ctccgaccct ccgctacaat gttggctccg
     5161 ccacaagcag accgcgaggg aatccatgga agtcaagctc gccgcagaga agcgtgagga
     5221 gtccgggaag ggtcccgccc gcagggcgcg ggccgcgggc cgcgtgcccg gggtcctcta
     5281 cggacctcag ctggaccccg tgaacttcac cgtcgacttg atggacctct ggcacgccct
     5341 ccacaccgcc gccggggtca acgtcctcgt cgacctgaag gtggacgggg aggagtacct
     5401 cacgatgccc cgcgaggtcc agcgcgatat cctcaaggga accctcctcc acgtggactt
     5461 catccagatc gatcgcaaca agccgatcca ggtccacgtc ccgatcgtcc tcaccggcga
     5521 ggccgaaggc cagaaggcgg gcggcgtcgt cgagcaccac ctgtgggagc tccacgtcga
     5581 ggtcctcccg ctggaggtgc cggagtcggt cgaggccgac atcaccgagc tcgagatgaa
     5641 cgacacgctc cacgtctcgg acctcacggt gcccgagggc gccacggtgc tcagctccga
     5701 ggaggagccg gtggtctcga tcgtgccgcc gccggtcatc gagctgcccg agcccgaggt
     5761 cgaggaggag gagctcctcg agggcgagga gggcgagctc gtggagggcg aggaggccgg
     5821 ggaggccgcc gagggcgagg gcggggaggc ccccgccgag gaaggcgagt ccgaggaagg
     5881 ctaggtgtcc gcctggctcg tcgtcgggct gggcaacccg ggcgaccgct acgcggagac
     5941 ccgtcacaac atcggagctt gggtcgtcga gcgcctctcc gatcgcctga acgcccgcct
     6001 ccggaaggtg cggttcatgc ccctgctggt cggcgaggcg accgtggacg gcgagcgcgt
     6061 ctatctcctg cggtcccaga ccttcatgaa cgtcaccggc ccgcccgtgg cgagcttcat
     6121 ccgcaagcgg aagctcgacc tggaccgcgt ggtcgtggtc cacgacgaga tcgacctgcc
     6181 cttcgggtcg ctcaaggtga agcagggcgg gtccacggcc ggccacaacg gcctgcgctc
     6241 cctcgtgggc gcgatcggct cgcccgactt cctgcgggtc cggctcggcg tggggaggcc
     6301 tccgggccgc caggaccccg ccgaccacgt cctgcgcccg ttcgccaagg ccgagcggga
     6361 ggatgcggag atcctggccg aggaggcggc cgacgcggtc ctcaccctga tccgggacgg
     6421 gctgagtgtc gcgcaggacc gccacaaccg ctccgtgcga tagtgccggg gtgagcgatc
     6481 ccaagcccag gcgccgtctg atcgagcgag ccatcctggg aacgttcatg ggcctcgccg
     6541 cgttcatcat cgagcgacgg gtgcggaagg cgcttcgccg ggccgaggcc gagcggggag
     6601 gagaccgatg agcaaacgaa cggtggagaa gacggacgag gagtggcgcc gggagctgac
     6661 cccggagcgg tacgcggtcc tgcgccagca ggccaccgag gcgccgttca cgagtgagct
     6721 gctgcaggtg aaggaggacg gcacgttccg ctgcgcggcg t
//
LOCUS       DASUTW010000096         3239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_28150, whole genome shotgun sequence.
ACCESSION   DASUTW010000096 DASUTW010000000
VERSION     DASUTW010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28150"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..507
                     /locus_tag="VF058_03045"
                     /pseudo
     CDS             1..507
                     /locus_tag="VF058_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020101463.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
     gene            514..861
                     /gene="rplQ"
                     /locus_tag="VF058_03050"
                     /pseudo
     CDS             514..861
                     /gene="rplQ"
                     /locus_tag="VF058_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            889..1617
                     /gene="truA"
                     /locus_tag="VF058_03055"
     CDS             889..1617
                     /gene="truA"
                     /locus_tag="VF058_03055"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX6207315.1"
                     /translation="MSVLKLVLAYDGTGFRGWARQPGKRTVQGVLERALERMLGEFPR
                     LSVAGRTDAGVHARGQVVSLEWEDPERLQRGLNGMLAPEVVVRRATRAPDGFDARRSA
                     RARVYRYRVSLAPIPDPFTARYVWHRPAPLALAPMRRAARLLEGEHDFASFCRAPRRG
                     GTVRRLERLAIRREGDLLELGVRANALCHQMVRSLVGTLLEVGEGRKEAGSMTEVLEA
                     RSRRAAGHVAPPHGLTLERVLYRP"
     gene            1634..2098
                     /gene="rplM"
                     /locus_tag="VF058_03060"
     CDS             1634..2098
                     /gene="rplM"
                     /locus_tag="VF058_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX6207316.1"
                     /translation="MKTYSPKPSDIERKWWLVDADGATLGRLASEVAQLLRGKHKPTW
                     APHVDMGDHVVVINASRIHLSGAKREQKVWYRHSQHPGGLRTIPYDRLLRERPDRAVE
                     KAVRGMLPKNRLGRQMIKKLHVYADAEHRHQGQSPEPLDLGAAVRRPTEEES"
     gene            2101..2490
                     /gene="rpsI"
                     /locus_tag="VF058_03065"
     CDS             2101..2490
                     /gene="rpsI"
                     /locus_tag="VF058_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX6207317.1"
                     /translation="MAEATRATGRRKESIARVRLTQGSGRFELNGRPLEEYFTSRVQR
                     MIVTAPLRAVEKEKELDVVASLEGGGASGQAGALQHGIARALVELDASLRGILKKEGF
                     LSRDAREKERKKYGLKKARKAPQYSKR"
     gene            complement(2491..2769)
                     /locus_tag="VF058_03070"
     CDS             complement(2491..2769)
                     /locus_tag="VF058_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207318.1"
                     /translation="MEPLVLIHTAANPTAGLLVKSRLESEDIPVFAKGELEGPYRVGP
                     VYLWVPRDLEVQARLVLAEILGGGLELPEGEDVVIEDERLETGPDQEA"
     gene            2825..>3239
                     /locus_tag="VF058_03075"
     CDS             2825..>3239
                     /locus_tag="VF058_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX6207319.1"
                     /translation="MARLFGTDGIRGVAGRELTTELAHGLGRALVATVRSKRPRVAIG
                     RDTRASGEMLEAALAAGICSAGGNVLLLGVVPTPAVAFLTVELGAHTGAVISASHNPP
                     QDNGIKLFSSDGMKLPDMVEDEIERLVREGGGEVAE"
BASE COUNT          492 a         1071 c         1256 g          420 t
ORIGIN      
        1 gagcggggcg tcggctggcg tcccgccgag cagaacaaga aggcgcggga cccgatcggc
       61 gtgatcccgg tcgactcgat cttctccccg gttcgccggg tgacgttcca gatcgagaac
      121 acgcgggtcg agcagatgac cgaccgcgag aagctgatcc tcgacgtcga gtccgacgga
      181 tcggtgagcc cccgcgaggc ggtggcctcg gcggggatca cgctgggcga gctcgtctcg
      241 ctcttcagcg acctcaccga ggcccccggc ctctccctcg ggcctcccga gggagaggag
      301 gagcccgtgg gcgagctggc gctccccatc gaggagctgc agctctcggt ccgctcctac
      361 aactgcctga agcgggaggg cgtcgatacg gtcggggagc tcgtccagag gaccgaggtc
      421 gagctgatgg acatccgcaa cttcggccag aagtcgatcg acgaggtcaa gtcgaagctc
      481 gacgagatgg gcctcgggct caaggaggag taggtgccga agccgaagaa gggaccgcgg
      541 ctcgggtcgg ggccggcgca ccaggggctg atgctcggcg gcctggcggc cgatctcttc
      601 acccacgagc ggctccggac cacggtggcg aaggcgaagg ccctccgccc ggtggccgag
      661 cgcctgatca cgttcgccaa gcggggcgac gtccacgcgc ggcgccaggt gctgaaggtg
      721 gtccccgatc gcgacgtcgt gcaccggctc ttcgacgaga tcgccccccg gttcgccgac
      781 cgggagggcg gctacacacg gatcctcaag ctcggccctc ggaagggcga cggggccgag
      841 atggcccaga tcgagctggt cgagcgcgag ggggagtaac ccgggccggt gtcggtcctg
      901 aagctcgtcc tggcctacga cgggacgggg ttccggggct gggcccgtca gcccgggaag
      961 cgcacggtcc agggggtcct ggagcgagcg ctggagcgga tgctggggga gttcccccgg
     1021 ctctccgtgg cgggacggac cgacgcgggc gtgcacgccc gggggcaggt cgtctcgctg
     1081 gaatgggagg atcccgagcg gctccagcgg gggctgaacg ggatgctcgc cccggaggtg
     1141 gtggtgcgca gggcgacgcg ggcgcccgac gggttcgacg cccggcggag cgccagggcc
     1201 cgggtgtatc ggtaccgggt gtcgctggcg ccgatcccgg acccgttcac ggcccggtac
     1261 gtgtggcacc ggccggcgcc gctggcgctg gcgccgatgc ggcgggcggc ccggctgctc
     1321 gagggggagc acgacttcgc ctcgttctgc cgggcgccga ggcggggcgg gacggtgcgc
     1381 cgcctggagc gtctggcgat ccgaagggag ggcgacctcc tggagctggg ggtcagggcg
     1441 aacgcgctct gccaccagat ggtgcgcagc ctcgtgggga cgctcctcga ggtgggagag
     1501 ggaaggaagg aggcgggctc gatgacggag gtgctggagg cgaggtcgcg gcgcgcggcc
     1561 ggccacgtgg cgcccccgca cgggctcacg ctggagcggg tactctatag gccgtgacct
     1621 ggggatacga gagatgaaga catacagccc gaagccgagc gacatcgagc ggaaatggtg
     1681 gctcgtcgat gccgacgggg ccacgctggg ccgtctggcc agcgaggtgg cccagctgct
     1741 ccgcgggaag cacaagccga cgtgggcccc tcacgtggac atgggcgacc acgtcgtggt
     1801 gatcaacgcc tcgaggatcc acctctcggg cgcgaagcgg gagcagaagg tctggtaccg
     1861 gcactcccag caccccggag gcctccgcac gatcccctac gaccggctcc tccgggagcg
     1921 ccccgaccgg gccgtggaga aggccgtgcg agggatgctg cccaagaacc ggctgggccg
     1981 ccagatgatc aagaagctgc acgtgtacgc ggacgcggag caccggcacc agggccagag
     2041 cccggagccg ctcgacctcg gcgccgcggt ccgccgtccg accgaggagg agtcctgagc
     2101 atggccgagg ccacgcgcgc cacggggcgc aggaaggagt cgatcgccag ggtccgtctg
     2161 acccagggca gcggccggtt cgagctcaac ggccgcccgc tcgaggagta cttcacctcg
     2221 agggtccagc ggatgatcgt cacggccccg ctccgggccg tggagaagga gaaggagctg
     2281 gacgtggtcg catcgctcga gggcgggggg gcgagcgggc aggccggcgc cctccagcac
     2341 gggatcgccc gggccctcgt cgagctggac gcttcgctcc gcgggatcct gaagaaggag
     2401 ggcttcctca gccgcgacgc ccgggagaag gagcggaaga agtacgggct gaagaaggcc
     2461 cggaaggccc ctcagtacag caagcggtag tcacgcctcc tggtcggggc ccgtctccag
     2521 gcgctcgtcc tcgatcacga cgtcttcccc ctcgggaagc tccaggcctc cccccaggat
     2581 ctcggccagc accagtctgg cctggacctc gaggtcgcgc ggcacccaga ggtacacggg
     2641 cccaacccgg taggggccct cgagctcgcc cttggcgaag acggggatgt cctcgctctc
     2701 gagcctgctc ttcaccagca gccccgcggt cgggttcgcc gccgtgtgga tgaggacgag
     2761 tggctccatg catccattct ggcatcgacg cggcgccggc ggcggggccg gtacgatacg
     2821 cggcgtggcg aggctcttcg gcaccgacgg gatccgcggc gtggcggggc gcgagctcac
     2881 gaccgagttg gcgcacgggc tggggcgggc gctcgtggcc accgtgcgga gcaagcggcc
     2941 ccgcgtggcg attgggcgcg acacgcgcgc ctctggcgag atgctcgagg ccgcgctggc
     3001 cgcgggcatc tgctccgcgg gcgggaacgt cctgctcctt ggggtggtcc ccacgccggc
     3061 cgtggcgttc ctcacggtgg agctcggggc ccatacgggc gcggtcatct ccgcgtccca
     3121 caaccctccc caggacaacg gcatcaagct cttctcctcc gacggcatga agctgcccga
     3181 catggtcgag gacgagatcg agcgcttggt gcgtgagggg ggcggcgagg tggccgagg
//
LOCUS       DASUTW010000097         3936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1453975, whole genome shotgun sequence.
ACCESSION   DASUTW010000097 DASUTW010000000
VERSION     DASUTW010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453975"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VF058_03080"
     CDS             <1..474
                     /locus_tag="VF058_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6207320.1"
                     /translation="LRVLVGGHQQVALDRAVAHGQGWIPGWRPFDELQEWIRLLRQKA
                     ETAGRDPSSSLIVAPQFSCLVAPTHEEATRRYMASGMVQHRKSLAYTGRDPGLAMDNN
                     LIGSPEAVLEKLARLERAGVDHLACINVLCGIPGGVRGAGSLLRPRGDGPVPWCY"
     gene            complement(639..711)
                     /locus_tag="VF058_03085"
     tRNA            complement(639..711)
                     /locus_tag="VF058_03085"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(676..678),aa:Arg,seq:cct)
     gene            952..3675
                     /locus_tag="VF058_03090"
     CDS             952..3675
                     /locus_tag="VF058_03090"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX6207321.1"
                     /translation="MKTLIDALAAVVRRAPWVVIVVTLAVTAFLGYQNQNFQPAEDQN
                     EAFAPEAPELVAAERIDELFGDESITSPMQVVIKGGDVITADGLAAVQAVTETMVTGS
                     LSEFLVEATPEQPNSVSFLSPVLAAVEAGAPAPTTDEEVKALYRQGLDALPPEQAGFI
                     EQLLPTAVDASEASSPSGLVITFYTGAASNDEFTEYAEASAAAAREIRGIPMPPGVTA
                     EPFSFELLFEGDDEFQAEIGRLFATAAFIILFVLSLVFLMRPRSGRDSVLRAVGIVGM
                     LLALVVLIVPGLALVLPDLFPDSFQDIETGPMLGIAALVYLVIFALWVFLSPRALRRT
                     TANTFLTMLTILVAITWMNGYGGLRFGSASPMAQILPILLIGLGVDYAIHMNSRYQEE
                     LASGASVDRGVMTAIRTVGIALVLATITTAVGFLTNVTNEIPALREFGELAAFGIVTS
                     FLLMLTFVPAIRELLDRRGERRETLDRAALQDGGERALPRLIGRAAALPLRAAVAVVI
                     VSIVLAGVGAFGVTRLEAKFSIVDFVPTTSPVRGTFETLLEDFGGGFGETTQILVEGD
                     VATPEAYNAMVQTTANLGDTENVLTFGDMAVVESPVSLVTSLSIPNTPSFDPGVAALA
                     AEAGISEEDPTVDPGADVAALYDAASAARPDLAATVLGDGGSYEAALFTIQTQAGEVG
                     ATQLREDLVEDVTPLASAGVDATATSDEIIGDVIINTLRDSQVSSLLITLAAALLLLV
                     VNFWVTSRRPMLGVITTVPVILVVLLSFTLMVVFGIAFGPVTATISALAIGIGIPYMI
                     HITHRYLEDRARHPSDGEAIGKTLVHTGGALAGSALTTIAGFGILVISTTIPFRQFGF
                     VTAYTIGLALLAAVLVLPSYLVLWDRWHRRRGEDPVDREAVEAALRMEVGT"
     gene            3695..>3936
                     /locus_tag="VF058_03095"
     CDS             3695..>3936
                     /locus_tag="VF058_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017310565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX6207322.1"
                     /translation="MRDAPAPRRLWRPPARFEERAPDRRVTFLELFFDLVFVVIIAQL
                     AHRLAEHPSWSGVGWFVFLFYAVWSSWINGTLYHDL"
BASE COUNT          570 a         1309 c         1384 g          673 t
ORIGIN      
        1 ttgcgggtgc tggtcggcgg acaccagcag gtggccctcg accgggctgt ggcccatggc
       61 caggggtgga tccctggatg gcggccgttc gacgaactcc aggagtggat tcggctcctt
      121 cggcagaagg ctgagaccgc cgggagagat ccctcctcgt cgttgatcgt ggctccgcag
      181 ttctcatgcc tggtcgcccc gacgcacgag gaggcaacga ggcggtacat ggccagtggg
      241 atggttcagc accggaagtc acttgcctat acgggccggg atcccgggct cgccatggac
      301 aacaacctga tcgggagccc cgaggccgtc ctggagaagc tcgcccgcct ggaacgggcg
      361 ggagtggacc atctggcgtg catcaacgtt ctgtgtggaa tcccgggagg agtacgagga
      421 gcaggttcgc ttctacgccc acgaggtgat ggacccgtac cgtggtgcta ctgacagcga
      481 atcggcgatg tcccggaggt gagtgggacg gggcgccact tgatggaggc gccaccgtca
      541 aggatcaggc gaaacacctg tcacgcatca gccggagcgg tgtcaggcct caggttacgc
      601 cggaacgtcg agcatcaccc gaaacacgac aacattcgcg cccccgggag gaatcgaacc
      661 tccgacaccc ggtttaggaa accggtgctc tatccactga gctacggggg cgatcctccc
      721 caagtctagc gggtgggcaa gcgctcccag gcggggcggc atccctcggc ctgcgctgcc
      781 acgccggtcg tctcctgggc aagcgatgag ttcggcgcgc ctcctgggtc gaagttcggg
      841 gaggaacggc gggggaatcg aaagcgcccc tggtctgttc gagatatgag aggcttggcg
      901 gccggcacgt cgcgtgtcgt cgggccgtac gtcacagaga gagggaggct cgtgaagacg
      961 ctcatcgacg ccctggctgc cgtcgtgcgc cgggccccgt gggtcgtgat cgtggtgacg
     1021 ctggccgtca ccgcgttcct tggctaccag aaccagaact tccagccggc ggaggatcag
     1081 aacgaggcct tcgcgccgga ggcgccggag ctcgtcgccg ccgagcggat cgacgagctc
     1141 ttcggtgacg agtccatcac gagccccatg caggtcgtga tcaagggcgg ggacgtgatc
     1201 accgcggacg gcctggccgc cgtccaggcc gtcacggaga cgatggtcac gggctcgctc
     1261 tcggagttcc tcgtcgaagc cacgcccgag cagccgaact ccgtcagctt cctctcgccg
     1321 gtgcttgccg cggtcgaggc aggggcgccc gcgccgacca ccgacgagga ggtcaaggcg
     1381 ctgtaccggc aggggctgga cgccctgcct ccggagcagg ccggtttcat cgagcagctg
     1441 ctgccgacgg cggtcgacgc atcggaagcg agctcgccca gcggcctggt catcaccttc
     1501 tacacggggg ccgcctcgaa cgacgagttc accgagtacg cggaggcctc tgcggcggcg
     1561 gcccgcgaga tccgcgggat cccgatgcct ccgggggtga ccgccgagcc cttcagcttc
     1621 gagctgctgt tcgagggcga cgacgagttc caggccgaga tcgggcggct gttcgccacc
     1681 gccgcattca tcatcctgtt cgtgctctcg ctggtgttcc tgatgaggcc ccgctcgggt
     1741 cgcgactcgg tccttcgcgc ggtcggcatc gtggggatgc tcctcgcgct cgtggtgctg
     1801 atcgtcccgg ggctggcgct cgtgctcccc gacctcttcc cggactcgtt ccaggacatc
     1861 gagaccggtc cgatgctggg gatcgcggca ctcgtgtacc tggtgatctt cgcgctgtgg
     1921 gtcttcctgt caccgcgggc gctgcgccga acgacggcga acaccttcct cacgatgctc
     1981 acgatcctgg tcgcgatcac ctggatgaac ggctacgggg gccttcgctt cgggtccgcc
     2041 agcccgatgg cgcagatcct cccgatcctg ctgatcggct tgggcgtgga ctacgcgatc
     2101 cacatgaaca gccggtacca ggaggagctc gcgagcggcg cgtcggtcga ccgaggggtc
     2161 atgactgcga tccgcacggt cggcatcgcg ctcgtgctcg ccacgatcac cacggcggtc
     2221 ggcttcctga cgaacgtaac gaacgagatc ccggccctcc gcgagttcgg cgagctggcg
     2281 gcgttcggga tcgtgacctc cttcctgctg atgctcacct tcgtgcccgc catccgtgag
     2341 ctcctcgacc gccggggaga gcgccgggag acgctcgatc gggccgcgct gcaggacggt
     2401 ggagagcggg ccctgcccag gctgatcggg cgggccgcgg ccctccctct gcgtgcggcg
     2461 gtggcggtgg tcatcgtctc gatcgtgttg gctggcgtcg gcgccttcgg ggtcactcgt
     2521 ctggaggcca agttcagcat cgtggacttc gtgcccacca cgtctcccgt ccgcggcacg
     2581 ttcgagaccc tgctggagga tttcggcgga ggatttggcg agaccaccca gatcctggtc
     2641 gagggggacg tggcgacgcc cgaggcctac aacgcgatgg tccagacgac cgcgaacctc
     2701 ggcgacacgg agaacgtcct gacgttcggc gacatggccg tggtcgagtc tcccgtgtcc
     2761 ctcgtgacca gcctctcgat cccgaacacg ccgtcgttcg atccgggggt ggcggccctg
     2821 gccgcggagg ccggcatctc cgaggaggat cccacggtgg atccgggagc cgacgtcgcc
     2881 gcgctgtacg acgcggcgtc cgcggcccgc cccgacctgg cggccacggt gctcggtgac
     2941 ggggggtcct acgaggccgc tctgttcacg atccagaccc aggcgggcga ggtcggagcg
     3001 acgcagctcc gcgaggatct ggtcgaggac gtgacgccgc tggcgtccgc cggtgtggac
     3061 gcgacggcca cgtccgacga gatcatcggc gacgtgatca tcaacacgct gcgggactcg
     3121 caggtctcgt cgctcctgat cacgctcgcc gcggcgctgc tgctcctggt cgtgaacttc
     3181 tgggtcacct ctcgcaggcc catgctgggc gtgatcacca cggtccccgt gatcctggtg
     3241 gtgctgctct cgttcacact gatggtcgtg ttcgggatcg ccttcgggcc cgtgacggcc
     3301 acgatctcgg ccctggcgat cggcatcggg atcccgtaca tgatccacat tacgcaccgc
     3361 tacctcgagg atcgggccag gcacccgagc gacggtgagg cgattggcaa gaccctcgtc
     3421 cacaccgggg gcgcgctggc ggggtcggcg ctcaccacga tcgcggggtt cgggatcctg
     3481 gtgatctcga ccacgatccc gttccggcag ttcgggttcg tgacggccta cacgatcggg
     3541 ctcgcgctgc tggcggcggt gctggtcctg cccagctacc tggtgctgtg ggaccggtgg
     3601 caccgccggc gcggcgagga cccggtggat cgagaggccg tcgaggcggc gctgaggatg
     3661 gaggtcggga cctgagggag ccgggggcct ccgcgtgagg gatgcgcccg ccccccggcg
     3721 tctgtggcgg cccccggcac ggttcgagga gcgggcgccg gaccggcgcg tgaccttcct
     3781 cgagctgttc ttcgacctcg tcttcgtggt catcatcgcg cagctggcgc accgactcgc
     3841 cgagcatccc tcgtggtcgg gcgtcggttg gttcgtcttc ctcttctacg cggtgtggtc
     3901 ctcgtggatc aacgggaccc tgtaccacga cctcca
//
LOCUS       DASUTW010000098         8123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_157029, whole genome shotgun sequence.
ACCESSION   DASUTW010000098 DASUTW010000000
VERSION     DASUTW010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8123
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157029"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VF058_03100"
     CDS             complement(<1..453)
                     /locus_tag="VF058_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207323.1"
                     /translation="MGEADRGGRAQEQAATNDVAAVLGQSTLIKAGRADVSRIRERTR
                     RRRLIRLALVIGLIDGYLWYRYATGNPIGLPSLPQDAWIWLPGLLLVALFAIVILLPM
                     GSGRSPAMVVRPEHIEVGLEELKGLDAQVDDVIRTLNVFLGYSTFRSRL"
     gene            627..995
                     /locus_tag="VF058_03105"
     CDS             627..995
                     /locus_tag="VF058_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207324.1"
                     /translation="MARKKREPVPTDAARHIGGLLRGVRKACGYRAVREAAQAKGCPA
                     ALQTIYAYERGGLLPSLPQFLELVEFYVGGDGVSDEARLRAVAAIHAALSSPAYHVPQ
                     AKELAARLHPAVDMGRRRTR"
     gene            995..1396
                     /gene="mce"
                     /locus_tag="VF058_03110"
     CDS             995..1396
                     /gene="mce"
                     /locus_tag="VF058_03110"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX6207325.1"
                     /translation="MLTDIDHVGIAVQDLEEAVERYRAAFGVEPTHRETVEDQGVNEV
                     MFKVGTSYIQLLGSRGPDTPVGKYLEARGEGIHHIAYRVDDVEEVLAHLREQGHRLVD
                     EHGRPGSRGTTVAFVHPKALHGTLIELVQEA"
     gene            complement(1393..1965)
                     /locus_tag="VF058_03115"
     CDS             complement(1393..1965)
                     /locus_tag="VF058_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207326.1"
                     /translation="MRRALVLTLALVVAACGGPEGAGDVATDVWVRDVCTTLTDWNDR
                     LQSRTGTLQTDLQGLPQGDFQALKDLMVSYIDGVIEDTDQAVTSLEGAGAPAVQDGEE
                     ASRLMVRGIGEVRTIFEEAREDIAALDVSRPRRFAMALQEIGSRIQQGGQRVQATFQE
                     VDQRGIGGEELDRAFSEEPTCAPLAGGAGP"
     gene            complement(1962..3140)
                     /gene="ccrA"
                     /locus_tag="VF058_03120"
     CDS             complement(1962..3140)
                     /gene="ccrA"
                     /locus_tag="VF058_03120"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HEX6207327.1"
                     /translation="MYAQVIRPERFGEPKDAFQIEEISVPEVGPDEALVLVMAAGINY
                     NNVWAAQGVPIDVTKVHGKLGEPDDFHIGGSDASGIVYAVGENVSNVKVGDHVVVHCG
                     AWDREADGLPDPMLHPSFRIWGYESNWGSFAQFCKAQGHQLLPKAPQLTWEEAAAPTL
                     VGATAYRMLFGWPPHVVEEGDVVLVWGAAGGLGSMAIQLVAQAGGKPIGVVSSEDKFE
                     FCTKLGAVGMINRKDFDHWGMLPHWTDDEAYATWAEGAKAFGKAMWDALGEKRSPKIV
                     FEHPGESTIPTSIFVADTGGMVVICAGTTGYNAVVDLRYLWTRQKRFQGSHFANDEQS
                     RAFNQMVIDGTVDPALSRTFSFADIGTAHQLMSENAHPDGNMAALVSAPSEGLSDVPA
                     "
     gene            3408..3692
                     /locus_tag="VF058_03125"
     CDS             3408..3692
                     /locus_tag="VF058_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207328.1"
                     /translation="MGELGSRGVTREELRHELRGLESRLVGIDSRMDGFESKLELLEA
                     RTDTKLANLESRMLERMTDQTRTFVRTFYVSNAAMVLAVATLAFGAARLA"
     gene            complement(3704..4627)
                     /locus_tag="VF058_03130"
     CDS             complement(3704..4627)
                     /locus_tag="VF058_03130"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX6207329.1"
                     /translation="MELTPVQQRTLDELMRMQPLPVFRRELVEGLRADLERRLRPLTD
                     NLRKGEGVWLSKSKLSDLHGRCEGLFLANHLGEGEFELGWQLARGALVHRAIAVSVNL
                     PDRSDAELVEAALAQLRRDDAAFDEFCLGLSDPDLAALESESVERVLLFRATFPPLQK
                     GWSPAVEPSLKATFAGDRVVLSARPDLLLGSDDREEPMRARRLVLELKTGSDRPEHDD
                     DLRFYALLATLRLGVPPFRVATVFLDDGTWRAHDVSEDLLEAAVRRIGDGAVRAGAII
                     AGEDPRLRPGPWCRWCPRTETCPESAFRADD"
     gene            4692..5114
                     /locus_tag="VF058_03135"
     CDS             4692..5114
                     /locus_tag="VF058_03135"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HEX6207330.1"
                     /translation="MRMYRSEDLTLLRLPPGTDIYEGVTEAAKELGIQGAQITAIGAL
                     RSLEYGYYDQVIQEYTHLTHEGELEIASANGNVSIRDGEPFLHMHIVAGKPDGTTIGG
                     HLFGGELFVAEVAFHLFRGEAPVREHDEEIGLPMWPTR"
     gene            5151..6728
                     /locus_tag="VF058_03140"
     CDS             5151..6728
                     /locus_tag="VF058_03140"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX6207331.1"
                     /translation="MAKRTEPTAEIEAKKLKELRRLHKKAEVAGGEEAIERQHARGKL
                     TARERIDLLLDPGSFVETDKLVKHRATDLGMARNRPYTDGVVTGWGTIDGRRVFVYSQ
                     DFTVFGGSLGEVHAEKICKVMDLAASVGAPVIGLNDSGGARIQEGASSLAGFGTIFDR
                     NVRFSGVIPQISAIMGPCAGGAVYSPAVTDFTYMVKETSHMFITGPDVIKTVTGEDVT
                     FEELGGAMTHASRSGLSSFVAEDEEECLQQIRYLLSFLPSNNMEDPPAYAPTDDPERR
                     VEELNAIVPDSAKEPYDMKDVVRGVVDGGEFYEVFPLWAMNVVVGFARLDGRSVGIVA
                     NQPKVLAGTLDIESSEKAARFIRFCDAFNIPLVTFVDVPGFLPGAAQEYGGIIRRGAK
                     LLYAFTEATVPRMTVITRKAYGGAYVVMNSKHIRADVSFAWPTAEIAVMGSGGAVNII
                     HRREIKEADDPEGTRAKLIERYEKSFLNPYRAAERGYVDDVIEPAETRIKLIQSLRML
                     HTKREQVPARKHGNIPL"
     gene            6731..6982
                     /locus_tag="VF058_03145"
     CDS             6731..6982
                     /locus_tag="VF058_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207332.1"
                     /translation="MPRYELPPDLSPEEERIVLAAIERMLELERPTLSPWVVAGRAEG
                     LRIGGLHIRHQTADPWTSHAPLPFAPGGTQRLPGRGDAR"
     gene            6979..7995
                     /locus_tag="VF058_03150"
     CDS             6979..7995
                     /locus_tag="VF058_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX6207333.1"
                     /translation="MTRARVALFSRKPTPGVFSIEQVFDLIAERLPDRFEPVKRVASY
                     RSKGVLPRALAVLEARRRQGDLNHVVGDVTFLALLLRRDRTIVTIHDCEFMERAGAVK
                     RWIYRWVWLELPARRAALVVVPTHGVREDLHRYTSVDPAKVRVISMPVDPRFRPSAMR
                     EDLSEPIILQVGTRPNKNLERVIRALEGVPCRLIVLGELTPEQRGLLGRCGVRFEERV
                     SLPRDEVHGLYRQSDLVVFASTKEGFGLPIAEAQASGRPVVAGDRPPLPEVAGEGACL
                     VDPYDVGSIRAGIERVLGDAPYRERLLAEGLRNVERFEAGKVAEAHAAVYDEVLASLP
                     ARAE"
BASE COUNT         1245 a         2817 c         2882 g         1179 t
ORIGIN      
        1 gagccgcgag cggaacgtcg agtacccgag gaacacgttg agcgtgcgga tcacgtcgtc
       61 gacctgggcg tcgaggccct tcagctcctc aagccccacc tcgatgtgct cgggccgcac
      121 gaccatggcc ggcgagcgtc cactccccat cgggaggagg atcacgatgg cgaagagggc
      181 gacgagcagg agccccggca gccagatcca cgcgtcctga ggtagcgagg gaaggccgat
      241 ggggttcccc gtcgcatacc ggtaccagag gtagccgtcg atcaggccga tcaccagcgc
      301 gagccggatc agtcggcggc gtcgagtccg ctccctgatc ctcgagacgt cggcccgtcc
      361 cgccttgatc agggtcgact gtccgaggac cgccgcgacg tcgttggtcg ccgcctgctc
      421 ctgcgccctg ccaccgcgat cggcctcgcc cacgtgccct ccatccgaga gacgacgatg
      481 cccgggccgc gcgcgccgcg ctcggcccgg gtcccacggg tagttgtacc tggaatccgc
      541 ggattccacc acctcctcga tttggcgatc gccgacccgg gcgggcagaa tcgcgccact
      601 ggagccctac tcgggaagga ccgccgatgg cacgaaagaa gcgcgaaccg gtccccacgg
      661 acgccgcccg ccacatcgga ggcctgctcc gaggagtacg gaaggcctgc ggctaccggg
      721 ccgtgcgaga agccgcccag gccaagggct gcccggccgc cctccagacg atctacgcgt
      781 acgagcgggg cgggctcttg cccagcctgc cccagttcct cgagctggtc gagttctacg
      841 tcgggggcga cggggtctcc gacgaagcca ggctccgcgc ggtcgccgcg atccacgccg
      901 ccctctcctc ccccgcgtac catgtcccgc aggccaagga gctggccgcg cgcctccacc
      961 cggcggtgga catgggacgg cggaggacga ggtagtgttg accgacatcg atcacgtggg
     1021 gatcgccgtc caggatctcg aggaggcggt ggagcggtac cgcgccgcct tcggcgtgga
     1081 gcccacccac cgcgagacgg tcgaggacca gggggtgaac gaggtgatgt tcaaggtggg
     1141 cacctcgtac atccagctgc tcgggtcccg aggcccggac accccggtcg ggaagtacct
     1201 cgaggcgcgc ggggagggga tccaccacat cgcctaccgc gtggacgacg tggaagaggt
     1261 cctcgcccat ctcagggagc aggggcaccg gctcgtcgac gagcacggtc gcccgggatc
     1321 acgcggcacg acggtggcct tcgtccaccc gaaggccctc cacggcacgc tgatcgagct
     1381 ggtgcaggag gcctaggggc cggcccctcc ggcgaggggc gcgcacgtgg gctcctcgct
     1441 gaaggctcgg tcgagctcct ctcccccgat cccgcgctga tcgacctcct ggaacgtggc
     1501 ctggacgcgc tgcccgccct gctggatgcg cgacccgatc tcctggaggg ccatcgcgaa
     1561 tcggcgaggg cgcgacacgt cgagcgccgc gatgtcctcc cgggcctcct cgaagatcgt
     1621 gcggacctcg ccgatcccgc ggaccatcag acgggaggcc tcctcgccgt cctgcaccgc
     1681 cggggcgccc gccccttcga gcgaggtcac ggcttgatcg gtgtcctcga tcacgccatc
     1741 gatgtacgaa accatgaggt ccttcagggc ctggaagtca ccctggggca gaccctggag
     1801 atccgtctgg agggtgccgg tcctgctctg cagtcggtcg ttccagtcgg tgagggtcgt
     1861 gcagacgtcg cggacccaga cgtcggtcgc gacgtccccc gccccttccg gaccgccgca
     1921 ggccgcgacg acgagggcga gcgtgaggac gagggcgcgc ctcacgcggg cacgtccgag
     1981 agcccctcgc tcggggcgga gacgagcgcg gccatgttgc cgtcggggtg ggcgttctcg
     2041 gacatcagct ggtgggccgt cccgatgtcg gcgaacgaga aggtgcgcga gagcgccggg
     2101 tccacggtgc cgtcgatcac catctggttg aaggcgcgcg actgctcgtc gttggcgaag
     2161 tgggagccct ggaaccgctt ctgccgggtc cagaggtagc ggaggtccac cacggcgttg
     2221 tagccggtgg tgccggcgca gatcacgacc atgccgccgg tgtcagccac gaagatcgag
     2281 gtggggatgg tggactcgcc ggggtgctcg aagacgatct tcgggcttcg cttctccccc
     2341 agggcatccc acatcgcctt gccgaacgct ttggccccct ccgcccaggt cgcgtaggcc
     2401 tcgtcgtcgg tccagtgagg cagcatcccc cagtggtcga agtccttgcg gttgatcatc
     2461 ccgacagcgc cgagcttcgt gcagaactcg aacttgtcct cgctggagac gaccccgatc
     2521 ggcttgccgc cggcctgggc cacgagctgg atcgccatcg acccgaggcc cccggccgcg
     2581 ccccagacaa ggacgacgtc gccctcctcg accacgtggg gcggccagcc gaagagcatc
     2641 cggtacgcgg tggcgcccac gagcgtcggg gctgccgcct cctcccatgt gagctggggc
     2701 gccttgggga ggagctggtg gccctgcgcc ttgcagaact gcgcgaacga cccccagttc
     2761 gactcgtagc cccagatccg gaacgagggg tggagcatcg ggtcgggcag gccgtcggcc
     2821 tcacggtccc atgcgccgca gtggacgaca acgtggtcgc ccaccttcac gttggagacg
     2881 ttctcgccca cggcgtagac gatgcccgag gcgtccgagc cgccgatgtg gaagtcgtcg
     2941 ggctctccca gcttgccgtg gaccttggtc acgtcgatcg ggacgccctg agcggcccag
     3001 acgttgttgt agttgatgcc ggcggccatc accaggacga gggcctcgtc gggcccgacc
     3061 tcggggacgg agatctcctc gatctggaac gcgtccttcg gctcgccgaa tcgctcgggc
     3121 cggatcacct gggcgtacat acgctggggg atctcgccca gcgacgggat ctcgccgatc
     3181 tcgtagatgt ccttggtcac gctcgctcct ccgtgggatg ggtgccctgg atgctaccca
     3241 aaggtaggtt cgctcatcca cccgggtcgt gtcggtgccg ggtgctacgg tcctgtccct
     3301 ccccgcttgt cgcttgccgg gaggaggcac agaatggccg tggatgagcg cgagcgcttg
     3361 cttcgcaagc tcgaggaggt tctcggtctc gaggaggcag gacgttgatg ggcgagctgg
     3421 gatcccgggg cgtcactcgc gaggagctcc gtcacgagct tcgtggcctg gagagtcgcc
     3481 tcgtcgggat cgattcgcgg atggacgggt tcgagtcgaa gctcgagctc ctcgaggcga
     3541 ggacggacac gaagctggcc aacctcgagt cgcggatgct cgagaggatg accgatcaga
     3601 cgcggacgtt cgtacggacc ttctacgtct cgaacgccgc gatggtgcta gccgtcgcca
     3661 cgctcgcctt cggcgccgcc cgcctcgcct gatccgagca gcctcagtcg tccgcgcgga
     3721 acgcggactc tgggcacgtc tcggtccggg ggcaccagcg gcaccacggg ccgggcctca
     3781 gccgcgggtc ctcgcccgcg atgatcgccc ccgcccgcac cgcaccgtcg ccgatgcggc
     3841 ggaccgccgc ctccagcagg tcctcgctca cgtcgtgcgc ccgccaggtg ccgtcgtcga
     3901 ggaacaccgt ggccacgcgg aagggcggca cgcccaaccg aagcgtcgcc agcagcgcgt
     3961 agaagcggag gtcgtcgtcg tgctccggcc ggtcgctccc cgtcttcagc tcgagcacca
     4021 gtcggcgggc ccgcatcggc tcctcgcgat cgtcgctccc cagcaggaga tccgggcggg
     4081 ccgacagcac cacgcggtcc cccgcgaacg tcgccttcag cgagggctcg acggcgggcg
     4141 accacccctt ctggagcggc gggaacgtgg cgcggaaaag cagcacccgc tccactgact
     4201 cggattccag cgcggcgagg tccggatcgg acaacccgag acagaactcg tcgaacgcgg
     4261 cgtcgtcgcg gcggagctgt gccagcgcag cctccacgag ctcggcatcc gaccggtcgg
     4321 gcaggttcac cgagaccgcg atcgcccggt gcacgagcgc cccccgggcc agctgccacc
     4381 cgagctcgaa ctccccctcc cccaggtggt tcgcgaggaa cagcccctcg caccggccgt
     4441 gcaggtccga gagcttcgac ttgctgagcc agacgccctc gcctttccgc aggttgtcgg
     4501 tgagcggccg gaggcggcgc tcgagatcgg cccgcagccc ctcgaccagc tcgcgacgga
     4561 acacggggag gggctgcatc cgcatcagct cgtcgagcgt gcgctgctgg accggcgtca
     4621 gctccatggg ctcagggtac ggcccgccac cgacatagga tgccccggaa ggccgaccga
     4681 aaggagcatc catgcgcatg taccggagcg aggaccttac cctgctgcgg ctccccccgg
     4741 gcaccgacat ctacgagggc gtcaccgagg cggccaagga gctggggatc cagggggccc
     4801 agatcacggc gatcggggcg ctccgcagcc tcgagtacgg ctactacgac caggtgatac
     4861 aggagtacac gcacctcacg cacgagggcg agctcgagat cgcctcggcc aacgggaacg
     4921 tttcgatccg ggacggcgag ccgttcctcc acatgcacat cgtggccggg aagcccgacg
     4981 ggaccacgat cgggggccac ctgttcgggg gcgagctgtt cgtggccgag gtcgcgttcc
     5041 acctcttccg tggggaggcc ccggtccggg agcacgacga ggagatcggc ctcccgatgt
     5101 ggcccacccg gtagactccg tcggtgcggc ggctggagat gggagcaggc atggcgaagc
     5161 gcacggaacc gacggcggag atcgaggcca agaagctgaa ggagctgcgg cgcctccaca
     5221 agaaggcgga ggtggccggc ggcgaggagg ccatcgagcg tcagcacgcc cgcgggaagc
     5281 tcaccgcgcg ggagcggatc gatctcctcc tcgatccggg ctccttcgtg gagaccgaca
     5341 agctcgtcaa gcaccgggcc accgacctgg ggatggcccg caaccgcccc tacaccgacg
     5401 gcgtcgtcac cggctggggc acgatcgacg gccggagggt cttcgtctac tcgcaggact
     5461 tcacggtctt cgggggctcg ctgggcgagg tccacgccga gaagatctgc aaggtgatgg
     5521 acctcgcggc gtcggtgggc gcgccggtga tcgggctcaa cgactcgggc ggggcccgga
     5581 tccaggaggg tgcgtcctcg ctggcggggt tcgggacgat cttcgatcgc aacgtgcggt
     5641 tcagtggcgt gatcccgcag atcagcgcga tcatggggcc ctgcgcggga ggcgccgtct
     5701 actcccccgc cgtcaccgac ttcacctaca tggtgaagga gacctcgcac atgttcatca
     5761 ctgggcccga tgtcatcaag accgtcacgg gcgaggacgt cacgttcgag gagctgggcg
     5821 gcgccatgac ccacgccagc cgctcgggcc tgtccagctt cgtggcggag gacgaggagg
     5881 aatgcctcca gcagatccgc tacctgctgt cgttcctccc ttcgaacaac atggaggacc
     5941 cgccggccta cgcgcccacg gacgaccccg agcgacgggt cgaggagctg aacgcgatcg
     6001 tccccgactc ggccaaggag ccctacgaca tgaaggacgt cgtgcgcggc gtcgtggacg
     6061 ggggtgagtt ctacgaggtc ttcccgctgt gggcgatgaa cgtggtcgtg ggcttcgccc
     6121 ggctcgacgg tcggagcgtg gggatcgtgg ccaaccagcc caaggtcctg gcgggcacgc
     6181 tcgacatcga gtcgagcgag aaggcggcgc gcttcatccg gttctgcgac gcgttcaaca
     6241 tcccgctggt gaccttcgtg gacgtccccg ggttcctgcc gggggcggcg caggagtacg
     6301 gcgggatcat ccggcgcggg gcgaagctcc tgtacgcctt caccgaggcc accgtgccgc
     6361 ggatgaccgt gatcacgcgg aaggcttacg ggggcgcgta cgtcgtgatg aactcgaagc
     6421 acatccgggc cgacgtctcg ttcgcctggc ccaccgccga gatcgcggtg atgggctcgg
     6481 gcggcgcggt gaacatcatc caccggcgcg agatcaagga ggccgacgac cccgagggca
     6541 cgcgggccaa gctgatcgag cggtacgaga agagcttcct caacccctac cgtgccgcgg
     6601 agcgcgggta cgttgacgac gtgatcgagc cggcagagac gcggatcaag ctgatccagt
     6661 cgctccgcat gctgcacacg aagcgcgagc aggtgcccgc tcgcaagcac ggcaacatcc
     6721 cgctctgacc atgccccgct acgagctgcc gcccgatctc tcgcccgagg aggagcggat
     6781 cgtcctcgcg gcgatcgagc gcatgctgga gctcgagcgc cccacgctct cgccgtgggt
     6841 cgtggccggg cgagccgagg gccttcgcat cggagggctc cacatccgcc accagaccgc
     6901 ggacccgtgg acctcgcacg cccccctgcc gttcgcgccc ggcgggaccc agcggctccc
     6961 ggggcgcggc gacgcacgat gacccgggcc cgggtcgccc tcttctcccg gaagcccacc
     7021 cccggcgtct tcagcatcga gcaggtcttc gacctgatcg cggagcggct gcccgaccgg
     7081 ttcgagcccg tgaagcgggt ggcctcctac cggagcaagg gggtgctgcc gcgagcgctg
     7141 gccgtgctgg aggctcggag gcggcagggc gacctcaacc acgtcgtggg cgacgttacc
     7201 ttcctcgccc tcctcctgcg acgcgatcga acgatcgtga cgatccacga ctgcgagttc
     7261 atggagcggg cgggcgccgt gaaacggtgg atctaccggt gggtgtggct ggagctcccg
     7321 gcgcggcggg cggcactggt ggtggtgccc acgcacggcg tgcgggagga cctccaccgg
     7381 tacacgagcg tggacccggc gaaggtccgg gtgatctcga tgccggtgga cccgcggttc
     7441 aggccgtcgg cgatgcggga ggatctgagc gagcccatca tcctccaggt ggggacgagg
     7501 cccaacaaga acctggagcg ggtgatccgg gcgctcgaag gggtgccgtg ccggctgatc
     7561 gtcctcgggg agctgacccc ggagcagcgg gggctgctgg gacgctgcgg ggtgcgcttc
     7621 gaggagcggg tctcgctccc tcgcgacgag gtccacgggc tgtaccggca gagcgacctc
     7681 gtcgtgttcg cctccacgaa ggaggggttc gggctgccga tcgccgaggc gcaggccagc
     7741 gggaggccgg tggtggccgg ggatcggcct ccgcttccgg aggtggccgg tgagggggcc
     7801 tgtctggtgg atccctacga cgtcgggtcg atccgcgcgg ggatcgagcg ggtgctcggg
     7861 gacgctccct accgggagcg gctgctcgcg gagggcctcc ggaacgtcga gcggttcgag
     7921 gccgggaagg tggccgaagc gcacgccgcg gtctacgacg aggtcctggc ctcgctcccg
     7981 gccagggccg agtaggtctc gcgcaggccc ctcgccgtcc ggtcccacgt gaagctcgcc
     8041 gcccgctccc tgccccgccg gatcaggctc tcgcgcagat cctcgtcgcc cagcacggcc
     8101 cggaggccct ccgcgatcgc gtc
//
LOCUS       DASUTW010000099         6118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1843736, whole genome shotgun sequence.
ACCESSION   DASUTW010000099 DASUTW010000000
VERSION     DASUTW010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1843736"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VF058_03155"
     CDS             <1..256
                     /locus_tag="VF058_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX6207334.1"
                     /translation="LTIYEDRSFTFVTKQPPAAELLKQAARVEKGSGEPNREKVGQVS
                     RSQVRQIAETKMKDLNARDLENAMRIIEGTARSMGIEVVG"
     gene            256..966
                     /gene="rplA"
                     /locus_tag="VF058_03160"
     CDS             256..966
                     /gene="rplA"
                     /locus_tag="VF058_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX6207335.1"
                     /translation="MGGKRYTDAMRSINREQLYTPADALRILHELPKAKFDETVELSF
                     RLGVDPRKAEEMVRGTVSLPHGTGQSVRVAAFAVGDKAREAQEAGADVVGGEDLLETV
                     LGGTIEFEAAVATPDMMPIVGKAGRILGPRGLMPNPKAGTVTEDIGKAVQDIKGGKLE
                     YRVDKAGNLHLIIGKRSFELQALSENYLTVVDEVLRAKPAGAKGRYIRSVTLSSTMGP
                     GIRIDPSKIKDVAESVPA"
     gene            1111..1815
                     /gene="rplJ"
                     /locus_tag="VF058_03165"
     CDS             1111..1815
                     /gene="rplJ"
                     /locus_tag="VF058_03165"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX6207336.1"
                     /translation="MRTTMPNQQKVEKVEEIKERLRSAQAAYFADYRGLSVPDIGEVR
                     TALLEAEAAFSVLKNTLTRLAVRDLEMSELESFLEGPTAVAFVQGDIVAGAKALVDAT
                     KRFPVLEVKGGLAEGRVLGADEIRALAALETREEMLAKVAGLLSMHMRKAAYLMQALQ
                     SRFASLLSAYREKLEEEAPAEEPPSEEAPAEEPPSEEPPAEEAPSEDEGGDDQASAEQ
                     AEEETEEAQDEEQEEG"
     gene            1816..2205
                     /gene="rplL"
                     /locus_tag="VF058_03170"
     CDS             1816..2205
                     /gene="rplL"
                     /locus_tag="VF058_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX6207337.1"
                     /translation="MAKLSSDDLLEAFKEMTLLELSEFIKQFEETFDVEAAAMAAPVA
                     VAGAPGGDAGAAAEEKDEFDVILSSAGDKKIQVIKEVRGLTNLGLKEAKELVDGAPKP
                     VLEKVNKETAEQAKEKLEAAGGTVELQ"
     gene            2362..5811
                     /locus_tag="VF058_03175"
     CDS             2362..5811
                     /locus_tag="VF058_03175"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX6207338.1"
                     /translation="MSLPNAVTGRERVSFAKLLEPQELPDLIAIQRESFDWLITDGLR
                     EVFDEISPIEDYTETYELRFGDFWFEDVKYSVDECKDKDMTYSAGLHVQAEFINKQTG
                     EIKSAPVFMGEFPMMTERGTFIINGTERVVVSQLVRSPGVYFDKQLDKTADKDIFIAK
                     IIPARGAWLEFEVDKKELVGVRIDRKRRQNVTVLLKALGWSSDEIMELFDNAPTIAAT
                     LEKDGLETPEEALEDIYRKLRPGEPPTAESARNLLENLFFNPKRYDLAKVGRYKVDKK
                     LGEVDDLLKTQLKRRYETLREQDKPERKEFEQPRWRVFSHPYGNERGAAPFKSVLTYE
                     DMVKTVRYLVRLHAGQEGFDTDDIDHFGNRRLRSVGELIQNQIRIGLSRMERVVRERM
                     TTQDVEAITPTTLVNIRPVVGSIKEFFGTSQLSQFLDQTNPLAGLTHKRRLSALGPGG
                     LSRERAGFEVRDVHPSHYGRMCPIETPEGPNIGLIGSLSSFGRMNDFGFVETPYRKVE
                     KGKVTDQIDYLTADEEDLYVIAQANAELDKNGRFVSDRVLVRRKGGEVDYVPSSEVDY
                     MDVSPKQIVSVGAAMIPFLEHDDANRALMGANMQRQAVPLLRAEAPLIGTGVELRAAV
                     DAGDVVMAEKAGTVEEVSADQIVVKERDGNLRPYKLQKYRRSNQGTCINQRPVVTEGD
                     RVAARQIIADGPSTDQGEMALGKNLLVAFMSWEGHNYEDAIVISERLAKEDVLTSIHI
                     EEHEVDARDTKLGPEEITRDIPNVSEEILKDLDERGIVRVGAEVTTGDYLVGKVTPKG
                     ETELTPEERLLRAIFGEKAREVRDTSLKVPHGESGKVIGVRVFTREDGDELSPGVNQL
                     VRVYVAQKRKISDGDKLAGRHGNKGVIAKVLPEADMPFLPDGTPVDIILNPLGVPSRM
                     NLGQVLETHLGWAAHQGFKSNGREHEGPVFVSSPVFDGAREHEIKEALATAKPNKDGD
                     QLVDEHGKAVLYDGRTGRPFEQPVTVGYIYILKLLHLVDDKIHARSTGPYSMITQQPL
                     GGKAQFGGQRFGEMEVWALEAYGAAYALQELLTIKSDDILGRVKVYEAIVKGENIPEP
                     GIPESFKVLIKEMQSLALNVEVLSAEGQEIEMRETDDDVFRAAEELGIDLSRREPAGA
                     DFE"
     gene            5831..>6118
                     /locus_tag="VF058_03180"
     CDS             5831..>6118
                     /locus_tag="VF058_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010986334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207339.1"
                     /translation="MLDVNYFDELRIGLATGDQIRAWSNGEVKKPETINYRTLKPEKD
                     GLFCERIFGPTRDWECHCGKYKRVRFKGIICERCGVEITRAKVRRERMGHIE"
BASE COUNT         1125 a         1947 c         2201 g          845 t
ORIGIN      
        1 gctgacgatc tacgaggacc gctcgttcac gttcgtcacg aagcagcccc ccgcggccga
       61 gctgctgaag caggccgcgc gggtggagaa gggctcgggc gagcccaacc gcgagaaggt
      121 ggggcaggtc tccaggagcc aggtccgcca gatcgccgag acgaagatga aggacctcaa
      181 cgcgcgggac ctcgagaacg cgatgcggat catcgagggc acggcgcgct cgatggggat
      241 cgaggtggtg ggctgatggg cggcaagcga tacacggacg cgatgaggtc catcaaccgc
      301 gagcagctct acaccccggc cgacgccctg cggatcctgc acgagctgcc gaaggcgaag
      361 ttcgacgaga ccgtcgagct ctcgttccgg ctcggcgtgg acccccggaa ggcggaggag
      421 atggtgcgcg gcaccgtctc cctgccccac ggaacgggcc agagcgtgcg cgttgccgcg
      481 ttcgcggtgg gcgacaaggc ccgcgaggcg caggaggccg gcgccgacgt cgtgggcggc
      541 gaggaccttc tggagacggt cctcggcggc acgatcgagt tcgaggcggc ggtagccact
      601 cccgacatga tgccgatcgt ggggaaggcc ggccgcatcc tcggcccccg cggcctgatg
      661 ccgaacccca aggccggcac ggtgaccgag gacatcggca aggcggtcca ggacatcaag
      721 ggtgggaagc tcgagtaccg agtggacaag gcggggaacc tccacctcat catcgggaag
      781 cgctcgttcg agctccaggc cctgtccgag aactatctga cggtggtgga cgaagtgctc
      841 cgggccaagc cggcaggcgc gaagggccgc tacatccgct cggtcacgct ttcctcgacg
      901 atgggcccgg ggatccgcat cgacccgtcc aagatcaagg acgtggccga gtcggtgccg
      961 gcctgaccgt tgtcggtccc ggtcgccgtg tgtatccttg gggccgccgc agacagcagg
     1021 ggcgggccga caaccgcgta atcccgacgg ggcacctgcc gaggcagcga agcgccttcc
     1081 cgggctgtgc tgcggaggcg cttctcgcat gtgaggacca cgatgccgaa ccagcagaag
     1141 gtcgagaagg tcgaggagat caaggagcgg ctgcgctccg cgcaggcggc gtacttcgcc
     1201 gactaccgcg gcctctccgt ccccgatatc ggggaggtcc gcacggccct cctcgaggcc
     1261 gaggccgcct tctcggtgct gaagaacacg ctgactcgcc tcgcggtccg ggacctcgag
     1321 atgtccgagc tggaatcctt cctcgagggg cccaccgcgg tggcgttcgt ccagggcgac
     1381 atcgtggccg gggcgaaggc cctcgtggac gccacgaagc gcttcccggt gctcgaggtg
     1441 aaggggggcc tggccgaagg tcgcgtcctt ggggccgacg agatccgggc gctcgcggcg
     1501 cttgagaccc gggaggagat gctggccaag gtggccggcc tgctctcgat gcacatgcgg
     1561 aaggccgcgt acctgatgca ggccctgcag tcgcggttcg cctcgctgct gagcgcctac
     1621 agggagaagc tggaggagga ggcccccgcc gaggagcccc cgagcgagga ggcccccgcc
     1681 gaggagcccc cgagcgaaga gccccccgcc gaggaggccc cgagcgagga cgagggggga
     1741 gacgatcagg cttccgccga gcaggcggag gaggagaccg aggaagcgca ggacgaggag
     1801 caggaggaag ggtagatggc caagctctcc agcgatgatc tcctcgaggc cttcaaggag
     1861 atgacgctcc ttgagctctc ggagttcatc aagcagttcg aggagacgtt cgatgtggag
     1921 gccgcggcga tggcggcccc cgtggcggtg gccggggcgc cgggagggga cgcaggcgcc
     1981 gcagccgagg agaaggacga gttcgacgtg atcctctcct ccgccgggga caagaagatc
     2041 caggtcatca aggaggtccg cggtctcacg aacctcggcc tgaaggaggc caaggagctc
     2101 gtggacggcg ctccgaagcc ggttctggag aaggtcaaca aggagacggc cgagcaggcc
     2161 aaggagaagc tcgaggccgc cggcgggacc gtcgagctcc agtaggtggc ggctcaccga
     2221 ccccgcccag cgggtcgccc cacggggcgg cctgcttcga ggcgcctccg ttgacactcc
     2281 gcgggcaggg gcctaaacta gcggtttgcc gtgccgtctc ccgcgtacgc gagttacacg
     2341 catgtcgttc cctagggggg gatctctttg ccgaacgccg tgaccggtcg tgagcgcgtg
     2401 tccttcgcca agcttctgga gccccaggag ctgcccgacc tgatcgcgat ccagcgcgag
     2461 tccttcgact ggctcatcac cgacggactg cgagaggtct tcgacgagat cagcccgatc
     2521 gaggactaca ccgagaccta cgagctccgg ttcggggact tctggttcga ggacgtcaag
     2581 tactccgtgg acgagtgcaa ggacaaggac atgacgtact cggcgggcct ccacgtccag
     2641 gccgagttca tcaacaagca gacgggcgag atcaagtccg ccccggtctt catgggggag
     2701 ttcccgatga tgaccgagcg cgggacgttc atcatcaacg ggaccgagcg ggtcgtcgtg
     2761 tcccagctcg tgcgctcgcc cggggtctac ttcgacaagc agctcgacaa gaccgcggac
     2821 aaggacatct tcatcgccaa gatcatcccc gcgcgcgggg cctggctcga gttcgaggtg
     2881 gacaagaagg agctcgtcgg cgtccgcatc gaccggaagc gccgtcagaa cgtcacggtg
     2941 ctcctcaagg ccctgggctg gtcgagcgac gagatcatgg agcttttcga caacgccccg
     3001 accatcgctg ccaccctgga gaaggacggg ctggagacgc ccgaggaggc tctggaggac
     3061 atctaccgga agctgcgccc gggcgagccg cccacggccg agtctgcccg caacctcctt
     3121 gagaacctct tcttcaaccc gaagcgctac gacctggcca aggtgggccg ctacaaggtg
     3181 gacaagaagc tgggcgaggt cgacgatctc ctgaagaccc agctgaagcg acgctacgag
     3241 acgctgcggg agcaggacaa gcccgagcgg aaggagttcg agcagcccag gtggagggtc
     3301 ttctcccacc cctacggcaa cgagcggggg gcggccccct tcaagtcggt cctcacctac
     3361 gaggacatgg tgaagacggt tcgctacctc gtgcgcctgc acgcgggcca ggagggcttc
     3421 gacaccgacg acatcgacca cttcgggaac cgccggctcc ggtcggtggg cgagctgatc
     3481 cagaaccaga tccggatcgg cctctcgcgg atggagcgcg tggtccgcga gcggatgacg
     3541 acgcaggacg tcgaggcgat cacgccgacg acgctcgtca acatccgccc ggtcgtgggc
     3601 tcgatcaagg agttcttcgg gacctcccag ctctcccagt tcctggacca gacgaacccg
     3661 ctggcggggc tgacccacaa gcgccgcctc tccgcgctgg ggccgggcgg gctctcgcgg
     3721 gagcgggcgg gcttcgaggt ccgggacgtg cacccgtctc actacggccg gatgtgcccg
     3781 atcgagacgc cggaggggcc gaacatcggc ctgatcggat cgctctcgtc cttcgggcgc
     3841 atgaacgact tcggcttcgt ggagaccccc taccggaagg tggagaaggg caaggtcacc
     3901 gaccagatcg actacctgac ggcggacgag gaggacctgt acgtcatcgc ccaggccaac
     3961 gccgagctcg acaagaacgg gcgcttcgtg agcgaccgcg tgctcgtccg gaggaagggc
     4021 ggcgaggtgg actacgtgcc gtcctcggaa gtcgactaca tggacgtttc gccgaagcag
     4081 atcgtctcgg tgggggcggc catgatcccc ttcctcgagc acgacgacgc gaaccgcgcc
     4141 ctgatgggcg cgaacatgca gaggcaggcc gtaccgctcc tgcgggcgga ggccccgctg
     4201 atcggcacgg gcgttgagct ccgcgcggcc gtggacgcgg gcgacgtcgt gatggccgag
     4261 aaggccggga ccgtcgagga ggtctccgcc gaccagatcg tggtcaagga gcgggacggc
     4321 aacctgcggc cgtacaagct gcagaagtac cgccgctcga accaggggac ctgcatcaac
     4381 cagcggcccg tggtgaccga gggcgaccgg gtcgcggcgc ggcagatcat cgccgacggc
     4441 ccctccaccg accagggcga gatggcgctg ggaaagaatc tcttggtcgc cttcatgtcc
     4501 tgggagggcc acaactacga agacgcgatc gtgatctccg agcgtctggc gaaggaggat
     4561 gtgctcacct cgatccacat cgaggagcac gaggtggacg cccgggacac gaagctcggg
     4621 cccgaggaga tcacgcgcga catccccaac gtctccgagg agatcctgaa ggacctcgac
     4681 gagcggggga tcgtgcgcgt gggggccgag gtcaccaccg gcgactacct cgtgggcaag
     4741 gtcacgccga agggcgagac cgagctgacc cccgaggagc ggctgctccg tgcgatcttc
     4801 ggcgagaagg cgcgggaggt ccgcgacacg tcgctgaagg tccctcacgg cgagtccggg
     4861 aaggtgatcg gggttcgcgt cttcactcgc gaggacggcg acgagctctc cccgggtgtc
     4921 aaccagctcg tgcgtgtcta cgtggcccag aagcggaaga tctccgacgg ggacaagctg
     4981 gccgggcggc acgggaacaa gggcgtgatc gccaaggtgc tgcccgaggc cgacatgccg
     5041 ttcctgcccg atgggacgcc ggtggacatc atcctgaacc cgctcggggt gccctcccgg
     5101 atgaacctgg gacaggtgct cgagacccac ctcggctggg cggcccacca ggggttcaag
     5161 tccaacggca gggagcacga gggccccgtg ttcgtctcgt ccccggtctt cgacggggcc
     5221 cgcgagcacg agatcaagga ggccctggcc acggccaagc ccaacaagga cggcgaccag
     5281 ctcgtggacg agcacggcaa ggcggtgctc tacgacgggc ggacggggcg gccgttcgag
     5341 cagccggtga cggtgggcta catctacatc ctgaagctgc tccacctggt ggacgacaag
     5401 atccacgccc ggtccaccgg cccgtactcg atgatcacgc agcagccgct gggcgggaag
     5461 gcgcagttcg gcggccagcg gttcggcgag atggaggtgt gggccctcga ggcctacggc
     5521 gcggcctacg ccctccagga gctgctgacg atcaagtcgg acgacatcct gggccgggtg
     5581 aaggtgtacg aggcgatcgt gaagggcgag aacatccccg agccggggat ccccgagtcc
     5641 ttcaaggtcc tcatcaagga gatgcagtcc ctcgcgctga acgtcgaggt gctctccgcg
     5701 gagggccagg agatcgagat gcgggagacc gacgacgacg tcttccgcgc ggcggaggag
     5761 ctgggcatcg acctctcgcg gcgtgagccg gcgggggcgg atttcgagtg agtcgagagg
     5821 agctgagcgc gtgctcgacg tgaactactt cgacgagctg aggatcggtc tcgccacggg
     5881 tgatcagatc cgagcctggt cgaacggcga ggtcaagaag ccggagacga tcaactaccg
     5941 caccctgaag cccgagaagg atgggctgtt ctgcgagcgc atcttcggcc ccacccgcga
     6001 ctgggagtgc cactgcggga agtacaagcg ggtccggttc aagggcatca tctgcgagcg
     6061 gtgcggcgtg gagatcaccc gcgccaaggt ccgtcgcgag cggatggggc acatcgag
//
LOCUS       DASUTW010000100        10175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1584079, whole genome shotgun sequence.
ACCESSION   DASUTW010000100 DASUTW010000000
VERSION     DASUTW010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10175
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584079"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VF058_03185"
     CDS             complement(<1..372)
                     /locus_tag="VF058_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEX6207340.1"
                     /translation="MLPYVGVLLATALTTLAATPVVRWLASRFGAIDRPSDRKVHPRP
                     TPTLGGVAIWLGILAGLWAASAIPSFRRVFSLSSEPLGVAIAGTVIVALGAFDDVRGM
                     AAPAKVAGQILAGGILILFGVQ"
     gene            complement(372..1628)
                     /gene="glyA"
                     /locus_tag="VF058_03190"
     CDS             complement(372..1628)
                     /gene="glyA"
                     /locus_tag="VF058_03190"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007464531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX6207341.1"
                     /translation="MSDWIVDWEALRATDPEVAEAILGELERERTTLRLIASENYTSP
                     AVLAALANPMNDKYAEGYPGRRYYGGCRWVDVTETLAIERAKELFGAEHANVQPHAGA
                     QANMAAYFAFMEPGDTLLGMSLPHGGHLTHGSPVNFSGKWFEVIGYEVDEDTELIDLD
                     KVRDLAKQYRPKVIVAGATAYPRVIDFAAFREIADEVGAILMVDAAHFIGLVAGGAYP
                     SPVPHADVVTFTTHKTLRGPRGAMILCREEHAKEIDRAVFPMMQGGPLEHTIAAKAVA
                     LKEAARPDFRDYAARVVRTARALAAGLQDEGLRLVSGGTDSHLALVDLQPIGVTGADA
                     EAACDEVGIALNKNAIPFDPLPPRTASGIRVGTPGPATLGMDEPEMKEVAEIIGEVLK
                     DPDSEEVKKGVRARVRELTARFPVYG"
     gene            complement(1625..2077)
                     /gene="rpiB"
                     /locus_tag="VF058_03195"
     CDS             complement(1625..2077)
                     /gene="rpiB"
                     /locus_tag="VF058_03195"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX6207342.1"
                     /translation="MRIAVGSDHAGFPLKEDLKGYLKELGHEVEDVGTDSEDPADYPA
                     FCAEVGRMVRDERVDRGIVLGGSGQGEQIAANKVRGVRAALCNDLFTAILSREHNDTN
                     VLAIGARIVAPTLAREILRVWLDTGFDGGRHQRRIEQIAEIEEEEARR"
     gene            complement(2074..2661)
                     /locus_tag="VF058_03200"
     CDS             complement(2074..2661)
                     /locus_tag="VF058_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HEX6207343.1"
                     /translation="MLRVLFVCTGNICRSPLAEAFLLDRARRLPEGLVSVRSAGTWGR
                     EGHPAMPETIVIGVEHGLDVGDHAATPLTADLIEGQDLLVGMARDHVAEIARMVPGSA
                     SRAFTLKELAHLLSALEEPPPRPDERAARKRIAAADELRRARGGPADADVADPIGLGH
                     DVYRTVAGEIEAAVDRVVEGLFGVRVSAGARGEGP"
     gene            2734..3369
                     /locus_tag="VF058_03205"
     CDS             2734..3369
                     /locus_tag="VF058_03205"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX6207344.1"
                     /translation="MASPKPRLRGRIHQVAFFVSFPAGASLVALADTPAARVVTFVYA
                     ASLTLLYGTSALLHRLDWRPRVLPRMRKLDHSAIFLLIAGTYTAFAFLALQGTWRIAI
                     LSIVWGGAAAGIVLKSISVKRFQVATAVLYIATGWVGIAAVPVFLPLLGAFRATLLVA
                     GGVLYTAGALVLLLRRPDPIPHVFGYHEVWHAMVVLASLCHYAVILLLVLG"
     gene            complement(3371..4540)
                     /locus_tag="VF058_03210"
     CDS             complement(3371..4540)
                     /locus_tag="VF058_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6207345.1"
                     /translation="MKVMPVTFDVGIVGAGIHGAAAAYHLSAKGLRTVLFERDAPAGG
                     PTGESSAICRAYYTNPFLAGCARDSIEMLRGFEGITGRDAEFRRTGLLFLHPPEDEER
                     VRESAGQLSDLGIPVEVLSREAVGERFPALDLTGIGIAAWEPEAGYADPVAATTGLVR
                     AAAEGGAEVRTGEAVASLERADRGGAIVETVGGDEVRCARLLIAAGPWTRSLAAQLGV
                     DLPLTVERHIVGAYRRVEGVDVPAHGDLLGGYYLRPEGDDLLLVGSLHAAPQVDPDDY
                     DRKAAREELERLAVPVIERVPGLEQASIHSGWASLYDVSPDWQPVIGEVAPEVFVDAG
                     TSGHGFKLAPALGRHVAGLVAGEEVDRGLAAFHPSRFAEGGAALVAGYGEARILG"
     gene            complement(4578..5186)
                     /locus_tag="VF058_03215"
     CDS             complement(4578..5186)
                     /locus_tag="VF058_03215"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX6207346.1"
                     /translation="MSEVVGPDEAGIEAAIRALDAGEAIVLPTDTVHGLGARPDHAAR
                     LFEIKGRPRDLTLPVLVATVEDAREVAVLDERAETLAASFWPGALTMVLGRATRSAAW
                     DLGIHEATVGVRIPAERVARTLLERTGPLAVTSANRSGEPTPEACEGVREMLGDDVAV
                     YLCTGTAPGGTPSTVVDLSGEPRVIRAGAIPIDDLLAALEAG"
     gene            complement(5183..6019)
                     /gene="prmC"
                     /locus_tag="VF058_03220"
     CDS             complement(5183..6019)
                     /gene="prmC"
                     /locus_tag="VF058_03220"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX6207347.1"
                     /translation="MDGAQVVADTARFLADHDVETPEAEAEILLAHLLRTDRAGLYAR
                     REDLTDEERDLLSRMLLVRASGVPLQHLTGDQPFFGLKLRVRPGVFIPRPETEVLVEQ
                     ALQCLRTESSPVVVDVGVGTGAVALAIKKERRDARVLATDASEDAVALAAENARQLSL
                     GIELLRGDLLSPLPGELRGMVDLIVSNPPYVTEEEYAQLPEVVRAEPKEALVGGTELH
                     ARLAREALAWLRPGAWLAMEIGAGQGDEVAGLLSERYERVEIVPDLAGLDRVARGRTP
                     EA"
     gene            6170..6913
                     /locus_tag="VF058_03225"
     CDS             6170..6913
                     /locus_tag="VF058_03225"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEX6207348.1"
                     /translation="MATTLYRTTERPRPEISADRPGDVLTKVLLACGVAYSVLYVVLN
                     DVIAATLYEGYSRTSQAVSELSATGAPTKGFLTAVLPLSTAVMTAFGVGVWRSARGRR
                     ALRVTGALLVAHALTFPLWLFAPMTSREEMVQGTMPANDLGHIVLTAATILFILGEIG
                     FGAAAFGRRFRIYSIATAATVLIFGALTGVESAKVPTGDPTPWMGLFERISIGPWLLW
                     LAVFAVALLRARSGRPSVPDTPAELVETA"
     gene            complement(6957..8024)
                     /gene="prfA"
                     /locus_tag="VF058_03230"
     CDS             complement(6957..8024)
                     /gene="prfA"
                     /locus_tag="VF058_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX6207349.1"
                     /translation="MRERLEEIERQYEELGRELSDPAVSSDPSKLRDLGRRHSELREI
                     VRAYREWRRADEDAEEARALAREEPDPEAAATWREEATRASARAKELEAEIEVLLLPK
                     DPNDERNVVLEIRAGTGGDEATLFARDLFEMYRRYAEARRWKTEVLSSSPSAIGGFKE
                     VVTEIRGQGAYSRLKHESGVHRVQRVPETESSGRIHTSTATVAVLPEAEDVDVDIRDE
                     DLHIDVYRSSGPGGQSVNTTDSAVRITHAPTGIVVSCQEERSQRQNREKAMRYLRARL
                     LKMAQEEQAAEEAAARKAQVGSGERSEKIRTYNFPQSRVTDHRIGLTSHRLEGILAGE
                     IDEFVDALLAEERARQLQSTG"
     gene            complement(8024..8950)
                     /locus_tag="VF058_03235"
     CDS             complement(8024..8950)
                     /locus_tag="VF058_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006554928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX6207350.1"
                     /translation="MSEKQHFYGGQAVLEGVMMRGAEVWAIAVRRPDGGIHRESHEID
                     SVAKRYPILRKPGLRGVIALGQALSIGVRALTISANHSVDEEEKLTPRQMAFSMIIAF
                     VFFIGLFIVGPAVLFGFARDQIPSGILLNVGEGIFRVALFLGYLLLIGRMKDIRRVFE
                     YHGAEHKTIAAYEHDEPLEPEAVDQYSTLHVRCGTNFLLIVMVLTIFVFTLFGTPGIW
                     WRIGSRILAIPLIAGVAFEALRIGARYPRSRVVRALMAPGLWLQKITTKPPSEDQIEI
                     AIASFEEVLRRERDVGEAPAAEGPVPIPEPED"
     gene            complement(9068..9283)
                     /locus_tag="VF058_03240"
     CDS             complement(9068..9283)
                     /locus_tag="VF058_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016875994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX6207351.1"
                     /translation="MKHYHINVFYSEEDDGYIADIPDLEACSAFGRTAEEAVAQVERA
                     KEAWLTTAREQGRPIPQPRYRPVGGSR"
     gene            complement(9280..9537)
                     /locus_tag="VF058_03245"
     CDS             complement(9280..9537)
                     /locus_tag="VF058_03245"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX6207352.1"
                     /translation="MGPRRLLERLRRGAVSNVRFRDLVRLVEALGFELQRTSGSHHIF
                     AHPAVPELLNLQDVAGEAKPYQIRQLLRLMERYSLSLEVEW"
     gene            complement(9621..9995)
                     /locus_tag="VF058_03250"
     CDS             complement(9621..9995)
                     /locus_tag="VF058_03250"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6207353.1"
                     /translation="MTPEALRSWLDAYGRAWETRDPEAAVELFSPDATYRETPFDEIM
                     EGREVIRQYWQQVPDYQRDIEFGYEIVAVEPVVVRWWSAYTKISSGDRVKLDGVFLLE
                     FDEAGFCTSLREWWHADETPAS"
BASE COUNT         1379 a         3843 c         3433 g         1520 t
ORIGIN      
        1 ctggacgccg aagaggatca ggatcccccc ggccaggatc tgcccggcga ccttggccgg
       61 ggccgccatc ccccgcacgt cgtcgaaggc gcccagcgcc acgatcacgg tgccggcgat
      121 cgccacgccg aggggctcgg aggagagcga gaagaccctg cggaacgagg ggatcgccga
      181 ggcggcccag agcccggcga ggatccccag ccagatcgcc acgcccccga gggtcggcgt
      241 gggcctcggg tgcaccttcc gatccgaggg acggtcgatc gcgccgaacc ggctcgccag
      301 ccaccggacc accggcgtcg ccgcgagggt ggtcagggcc gtcgccagga ggacgcccac
      361 gtagggcagc actacccgta cacggggaag cgcgcggtca gctcccgcac ccgcgcgcga
      421 acccccttct tcacctcctc ggaatccggg tccttcagga cctcgccgat gatctcggcc
      481 acctccttca tctccggctc gtccatcccc agcgtggccg gccccggcgt ccccacccgg
      541 atccccgagg cggtcctggg gggcagcgga tcgaagggga tggcgttctt gttgagggcg
      601 atgccgacct cgtcgcaggc cgcctccgcg tcggcccccg tcaccccgat cggctggagg
      661 tccacgagcg cgaggtgcga gtcggtcccg cccgagacga gccgcaggcc ttcgtcctgg
      721 agacccgccg ccagcgcgcg ggccgtgcgg acgacgcgcg ccgcgtagtc gcggaagtcc
      781 ggccgggccg cctccttcag ggcgacggcc ttggccgcga tcgtgtgctc gagcgggccg
      841 ccctgcatca tcgggaagac cgcccggtcg atctccttgg cgtgctcctc acggcacagg
      901 atcatcgcgc cgcggggtcc gcgcagggtc ttgtgggtag tgaaggtcac gacgtcggcg
      961 tgcggcaccg ggctggggta ggcgccgccg gccacgaggc cgatgaagtg cgccgcgtcc
     1021 accatgagga tcgcccccac ctcgtcggcg atctcgcgga aggccgcgaa gtcgatcacg
     1081 cgcgggtacg cggtggcccc ggccacgatc accttgggtc ggtactgctt ggccaggtcc
     1141 cggaccttgt ccaggtcgat cagctcggtg tcctcgtcca cctcgtagcc gatcacctcg
     1201 aaccacttcc ccgagaagtt caccggcgac ccgtgcgtga ggtggccgcc gtggggcagg
     1261 ctcatgccga gcagggtgtc gcccggctcc atgaacgcga agtaggccgc catgttcgcc
     1321 tgggcccccg cgtggggctg gacgttggcg tgctcggccc cgaacagctc cttggcccgc
     1381 tcgatggcca gcgtctcggt gacgtccacc cagcgacagc cgccgtagta gcggcgaccc
     1441 gggtagccct cggcgtactt gtcgttcatc gggttggcca gggccgccag cacggccggg
     1501 ctcgtgtagt tctcggaggc gatcaggcga agggtggtgc gctcgcgctc cagctcgccg
     1561 aggatcgcct cggccacctc ggggtcggtc gcccggaggg cctcccagtc gacgatccag
     1621 tcgctcatcg cctcgcctcc tcctcttcga tctccgcgat ctgctcgatg cgccgctggt
     1681 gacgaccgcc gtcgaacccc gtgtcgagcc agacccgcag gatctcgcgg gccagcgtcg
     1741 gggcgacgat ccgggcgccg atcgccagca cgttcgtgtc gttgtgctcc cgggagagga
     1801 tcgccgtgaa gaggtcgttg cagagggcgg cgcggacgcc gcgcaccttg ttcgcggcga
     1861 tctgctcgcc ctggcccgat cctcccagga cgatgccgcg gtcgacgcgc tcgtcccgca
     1921 ccatgcggcc gacctccgcg cagaacgccg gatagtcggc ggggtcctcc gagtcggtcc
     1981 ccacgtcctc cacctcgtgg cccagctcct tcaggtaccc cttcaggtcc tccttgagcg
     2041 ggaaccccgc gtggtccgag ccgaccgcga tcctcacggt ccctcccccc gcgccccggc
     2101 cgagacccgg accccgaaca gcccctcgac gacccggtcc accgcggcct cgatctcgcc
     2161 ggccaccgtc cgatagacgt cgtgccccag cccgatcgga tcggccacgt cggcgtcggc
     2221 gggtccccct cgggcccggc ggagctcgtc ggccgcggcg atcctctttc gggcggcacg
     2281 ctcgtccggc ctcgggggag gctcctccag agcgctcagc aggtgcgcca gctccttcag
     2341 cgtgaaggcc cgcgaggccg atcccgggac catccgggcg atctcggcca cgtggtctcg
     2401 ggccatcccc acgaggaggt cctgcccctc gatcaggtcg gcggtcaggg gggtcgccgc
     2461 gtggtctccg acgtcgaggc cgtgctccac gccgatcacg atcgtctcgg gcatcgcggg
     2521 gtgaccctcc cgcccccagg tcccggcgga gcggaccgac accagccctt cggggaggcg
     2581 gcgggcccgg tcgagcagga acgcctcggc caacggggag cgacagatgt ttccggtgca
     2641 gacgaagagg acccggagca cgaaaggagt gtagagcatc ggccgcggga gccggccgga
     2701 gcatcgagcc cgtcctgctg gtagcatcgc aagatggcct cgccgaagcc gcggctccgt
     2761 ggccggatcc atcaggtcgc gttcttcgtg tcgttccccg ccggagcctc gctcgttgct
     2821 ttggccgata cgccggccgc cagggtcgtc acctttgtct acgcggcgag cctcaccctc
     2881 ctctacggga cgagcgcgct cctgcaccgg ctcgactggc gaccccgcgt gctcccccgg
     2941 atgaggaagc tcgaccactc ggcgatcttc ctgctgatcg ccgggaccta caccgcgttc
     3001 gccttcctcg cgctccaggg gacctggagg atcgcgatcc tctcgatcgt ctggggcggc
     3061 gcggccgccg ggatcgtcct caagagcatc agcgtgaagc ggttccaggt cgccaccgcc
     3121 gtcctgtaca tcgcgaccgg gtgggtgggc atcgcggccg tcccggtctt cctccctctg
     3181 ctgggagcgt tccgagccac cctgctggtg gcggggggcg tcctctacac cgccggcgcc
     3241 ctggtgctcc tcctgcggcg ccccgacccc atcccccacg tgttcgggta ccacgaggtc
     3301 tggcacgcca tggtggtgct ggcgagcctg tgccactacg ccgtgattct gctgctcgtc
     3361 ctgggctgag tcaccccagg atccgggcct cgccgtagcc ggccacgagc gctgctcctc
     3421 cctccgcgaa gcgagaggga tggaaggccg ccagcccgcg gtcgacctcc tctccggcca
     3481 ccaggcccgc gacgtggcgg ccgagcgccg gggcgagctt gaagccgtgg ccgctcgtcc
     3541 ccgcgtccac gaagacctca ggagcgactt ccccgatcac gggctgccag tcggggctga
     3601 cgtcgtagag gctggcccag cccgagtgga tcgaggcctg ctccagcccg gggacccgct
     3661 cgatcaccgg gacggccagg cgctcgagct cctcgcgcgc ggccttccgg tcgtagtcgt
     3721 ccggatcgac ctgcggcgcc gcgtggagcg agccgacgag gaggagatca tcgccctcgg
     3781 ggcgcaggta gtagccgccg agcaggtcac cgtgcgccgg cacgtccacg ccctcgaccc
     3841 gccggtaggc gcccacgatg tgccgctcga ccgtgagggg gaggtccacg ccgagctgcg
     3901 cggcgagcga gcgcgtccag gggccggccg cgatcaggag acgggcgcat cggacctcgt
     3961 cgccgccgac cgtctcgacg atcgcgccgc cacggtccgc ccgctcgagc gaggccaccg
     4021 cctctcccgt tcgcacctct gctccgcctt ccgccgcggc ccggacgagg cccgtcgtgg
     4081 cggccacggg atcggcgtac ccggcctcag gctcccaggc ggcgatcccg atccccgtca
     4141 ggtcgagcgc ggggaaccgc tcccccaccg cctcccggct cagcacctcg acggggatcc
     4201 cgagatcgga cagctggccg gcgctctccc gcacccgctc ctcgtcctcg ggagggtgga
     4261 ggaagaggag gccggtcctt cggaactccg cgtcccgccc cgtgatcccc tcgaagccgc
     4321 ggagcatctc gatgctgtcc ctggcacagc cggccaggaa cgggttcgtg tagtaggccc
     4381 gacagatcgc gctggactcg ccggtgggac caccggccgg cgcatcgcgc tcgaagagga
     4441 cggtccgaag gcccttcgcc gagaggtggt aggcggcggc agcgccgtgg atgccggccc
     4501 cgacgatccc cacgtcgaac gtgacgggca tgaccttcat cgtggaggct ccggccccgc
     4561 ggggcaaccc ccgggcgtca gcccgcttcc agcgcggcga ggaggtcgtc gatcgggatc
     4621 gccccggccc ggatcacgcg gggctccccg ctgaggtcga ccaccgtgga gggcgtgccc
     4681 ccgggcgcgg tccccgtgca gaggtagacg gcgacgtcgt ccccgagcat ctcgcggacc
     4741 ccctcgcacg cctcgggcgt gggctccccg gagcggttgg cgctcgtcac ggcgagggga
     4801 ccggtgcgct cgaggagggt gcgcgcgacg cgctcggcgg ggatgcggac gccgacggtc
     4861 gcctcgtgga tgccgaggtc ccacgccgcc gagcgcgtcg cgcgccccag gaccatcgtc
     4921 agcgcccccg gccagaacga ggccgccagc gtctcggccc gctcgtcgag cacggccacc
     4981 tcccgcgcgt cctccaccgt cgccaccagc accgggagcg tcagatcccg gggccgccct
     5041 ttgatctcga agagccgcgc cgcgtggtcg ggcctggccc ccagcccatg caccgtgtcg
     5101 gtggggagca cgatcgcctc gccggcgtcc agcgctcgga tcgcggcctc gatccccgcc
     5161 tcgtcgggcc ccacgacctc gctcatgcct ccggcgttcg cccccgcgcc acccggtcca
     5221 gcccggccag gtcggggacg atctccacgc gctcgtaccg ctccgacagc aggccggcca
     5281 cctcgtcgcc ctggcccgcc ccgatctcca tcgccagcca ggccccgggg cggagccagg
     5341 cgagcgcctc ccgggccagc ctggcgtgga gctcagtacc ccccaccagc gcctccttgg
     5401 gctcggcgcg gaccacctcc ggcagctgcg cgtactcctc ctcggtgacg tacggggggt
     5461 tgctcacgat gagatccacc atccctcgga gctcgcccgg cagcggcgag agcagatcgc
     5521 cccggagcag ctcgatcccg aggctcagct gcctcgcgtt ctccgccgcc agcgccactg
     5581 cgtcctccga cgcgtcggtg gccagcaccc gcgcgtccct ccgctccttc ttgatcgcca
     5641 gcgccaccgc ccccgtgccc acccccacgt ccacgaccac cggcgacgac tcggttcgca
     5701 ggcactggag ggcctgctcc acgaggacct cggtctcggg cctggggatg aacacgccgg
     5761 gccgaacccg gagcttgagc ccgaagaagg gctgatcacc ggtgaggtgc tgcaagggga
     5821 cccccgacgc gcgaaccagc agcatgcgag agaggaggtc gcgctcctcg tccgtcaggt
     5881 cctcgcgccg agcgtagagc ccggcgcgat ccgtcctcag gaggtgcgcc aggaggatct
     5941 ccgcctcggc ctccggcgtt tccacgtcgt ggtcggccag gaaccgggcg gtatcggcga
     6001 cgacctgggc cccatccatg aaggcagtct atcgacgcgc cttcgggtcc ccttgagggc
     6061 ggggcccaca ggacctcccc ttcggggagc ggtcggccct acccacgccc cgctcgcagg
     6121 atgagactga gcatggttgc gcgtcgatcc acgcgcggag gaggcagcga tggctaccac
     6181 actgtacagg acgacggagc gaccgcggcc cgagatctca gccgaccgcc cgggagacgt
     6241 cctgacgaag gtccttctcg cctgcggcgt cgcgtactcg gtgctctacg tcgtcctcaa
     6301 tgacgtgatc gcggcgaccc tctacgaggg gtacagccgc acgtcgcagg ccgtgagcga
     6361 gctctccgcg acgggggcgc cgacgaaggg cttcctcacc gcggtgctcc ccctgtccac
     6421 cgcggtcatg accgcgttcg gggtgggggt gtggaggtcg gctcggggga ggcgcgcgct
     6481 gcgcgtgacc ggagccctgc tggtcgccca cgcgctcacc ttcccgctct ggctgttcgc
     6541 cccgatgacc tcccgtgagg agatggtgca ggggacgatg cccgcgaacg acctcgggca
     6601 catcgtcctc accgccgcca cgatcctgtt catcctggga gagatcgggt tcggggccgc
     6661 ggccttcggc cgtcggttcc gcatctactc gatcgccacg gcggcgacgg tgctgatctt
     6721 cggagcgctc acgggcgtgg agtcggcgaa ggtcccgacc ggcgacccca ccccgtggat
     6781 ggggctcttc gagcggatca gcatcgggcc gtggctcctg tggctcgccg tcttcgccgt
     6841 cgccctcctg agggctcgga gcggcaggcc gtcggttccg gacaccccgg cggagctcgt
     6901 cgagacggcg tgacatcccc cggtcggggg tcgcttccct ccccccggcc ggggactcag
     6961 ccggtcgact gcagctggcg ggcccgctcc tcggccagca gggcgtccac gaactcgtcg
     7021 atctcgccgg ccaggatccc ctcgagccgg tgcgaggtca gcccgatccg gtggtcggtg
     7081 acgcggctct gggggaagtt gtaggtccgg atcttctccg agcgctcccc cgaacccacc
     7141 tgagccttcc gcgccgcggc ctcctcggcc gcctgctcct cctgcgccat cttcagcagt
     7201 cgcgcccgca ggtaccgcat cgccttctcc cggttctggc gctgggaccg ctcctcctgg
     7261 cacgacacca cgatccccgt gggcgcgtgc gtgatccgga ccgccgagtc ggtcgtgttg
     7321 accgactgcc cacccggccc cgaggaccgg tacacatcga tgtgcaggtc ctcgtcgcgg
     7381 atgtcgacgt ccacgtcctc ggcctcgggc aggaccgcca cggtggccgt cgacgtgtgg
     7441 atgcggccgc tggactcggt ctcggggacc cgctggaccc ggtgcacgcc ggactcgtgc
     7501 ttcaggcgcg agtacgcgcc ctgcccccgg atctccgtga cgacctcctt gaagcctccg
     7561 atcgccgagg ggctcgacga gagcacctcg gtcttccagc gccgcgcctc cgcgtagcgg
     7621 cggtacatct cgaagaggtc gcgggcgaag agggtcgctt cgtcgccgcc ggtccccgcg
     7681 cggatctcca ggacgacgtt gcgctcgtcg ttggggtcct tcgggagcag gaggacctcg
     7741 atctcggcct cgagctcctt ggcgcgcgcg ctcgcccgcg tggcctcctc acgccaggtc
     7801 gcagcggcct cggggtcggg ctcctcgcgg gcgagcgcgc gcgcctcctc ggcgtcctcg
     7861 tccgcccgcc gccactcgcg gtaggctcgc acgatctcgc gcagctccga gtgccgcctc
     7921 cccaggtcgc ggagcttgct cgggtcggag gagacggccg ggtccgagag ctcccgaccc
     7981 agctcctcgt actgccgctc gatctcctcg aggcgctcgc gcatcagtcc tcgggctcgg
     8041 ggatcgggac cggcccctcc gcggccggag cctctcccac gtcccgctcc cgccgcagga
     8101 cctcctcgaa ggacgcgatc gcgatctcga tctggtcctc ggagggaggc ttcgtcgtga
     8161 tcttctgcag ccacaggccc ggggccatca gcgcccgcac cacccgcgac ctcggatacc
     8221 gcgccccgat gcgcagggcc tcgaacgcca ctccagcgat gagcgggatg gccaggatcc
     8281 tcgacccgat cctccaccag atccccggcg tcccgaagag cgtgaagacg aagatcgtga
     8341 gcaccatcac gatcagcagg aagttcgtgc cgcaccgcac gtggagggtc gagtactggt
     8401 ccaccgcttc gggctccagc ggctcgtcgt gctcgtaggc cgcgatcgtc ttgtgctcgg
     8461 ccccgtggta ctcgaacacc ctccggatgt ccttcatccg cccgatcagc aggaggtacc
     8521 cgaggaacag cgccacccgg aagatcccct ccccgacgtt caggaggatc ccgctcggga
     8581 tctggtcccg ggcaaacccg aacagcaccg ccggccccac gatgaacagc ccgatgaaga
     8641 agacgaaggc gatgatcatc gagaacgcca tctgtcgagg ggtgagcttc tcctcctcat
     8701 ccaccgagtg gttggcggag atcgtgaggg ctctcacccc gatcgagagg gcctggccca
     8761 gcgcgatcac cccgcgcagg ccgggcttgc gcaggatcgg gtagcgcttc gccaccgaat
     8821 cgatctcgtg cgactcgcgg tggatccccc cgtcgggcct gcggacggcg atcgcccaga
     8881 cctcggctcc ccgcatcatc acgccctcga ggacggcctg gccgccgtag aagtgctgct
     8941 tctcgctcac cggcccattg tacgggcgac ctcgtccgac atcgctcgct acccttcgag
     9001 gagatgcggt aggcctcggt tcccatccac gaggatcagc ctccttcggc cgcggggcca
     9061 gatcccgcta gcgggatcca cccacgggtc ggtagcgcgg ctggggaatg ggccgaccct
     9121 gctccctcgc cgtggtcagc cacgcctcct tcgcccgctc gacctgggcg accgcttcct
     9181 ccgccgtcct gccgaaggcc gagcaagcct ccaggtcagg gatatcggcg atgtagccat
     9241 cgtcttcctc gctgtagaac acgttgatgt gatagtgctt caccactcga cctccaggct
     9301 cagagaatag cgctccatca gccgaaggag ctgccggatc tggtagggct tcgcctcgcc
     9361 cgccacgtct tgcaggttca gaagctccgg cacagccgga tgagcgaaga tgtggtgact
     9421 tccactcgtc cgttggagct cgaaacccaa tgcctctacc agcctcacaa ggtcccggaa
     9481 tctgacgttg ctgacggcac ctctgcgaag tcgttcgagc agcctgcgcg ggcccatcgt
     9541 cggaccttat ccaataggtc tgacatccag cccagaacgg cggtgctccg acgaagctgg
     9601 acgggcgata ctcgagggct tcaggacgct ggggtctcgt cggcgtgcca ccactcgcgg
     9661 agcgaggtgc agaacccggc ctcgtcgaac tccaggagga agacgccgtc gagcttcacg
     9721 cggtcgccgc tgctgatctt cgtgtaggcg gaccaccagc ggacgactac gggctcgacg
     9781 gccacgatct cgtagccgaa ctcgatgtcg cgctggtagt cggggacctg ctgccagtac
     9841 tgacggatca cctcgcgccc ctccatgatc tcgtcgaaag gcgtctcgcg gtaggtggcg
     9901 tcgggcgaga acagctcgac cgcggcctcc gggtctcgcg tctcccaggc gcgtccgtag
     9961 gcgtccagcc aggacctgag ggcctcgggg gtcacgcggc ggctactcgg cggtcggctc
    10021 ggaggcttcg gctacggccg gggattctgc cggggcctcg gccccggcgt cgaccggagc
    10081 ctccgcctcc tcctgcggcc ggcgccgcag agcgctgggg gccggacgcc cctcatagcg
    10141 gcggcggaag cgctcgaccc ggccgcccga gtcca
//
LOCUS       DASUTW010000101         4328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_806154, whole genome shotgun sequence.
ACCESSION   DASUTW010000101 DASUTW010000000
VERSION     DASUTW010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806154"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VF058_03255"
     CDS             <1..1015
                     /locus_tag="VF058_03255"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207354.1"
                     /translation="PATPTVVKQIPRDPGTHSHKVVVGDGLMLANCERDLVWEGPPFV
                     EPTGGADEWNPGLRVYDASDPADPKEIAFYQTEGTGVHRMTFEPPYVYMSGSDEGFTE
                     QFLIILDLSDPAKPVEAGRWWYPGMWTGGGERPRFPPDRRYALHHGLLDGDLVFCGWW
                     DAGLVILDVADRSTPQLVANLNWGPSESGATHTALPLHGRDLVVVTDEAIVDECRDVT
                     KQVRVLDVSDPYTPKEVSRLPIPDGNFCERGGRFGPHNLAEPRPFTKVETDRVYVAYF
                     NAGLRVIDLEDPERPREIAHLIPDPPPGKPTSMVDDVAVADDGTIYLTDRAGGGLHVV
                     GWA"
     gene            1012..2181
                     /locus_tag="VF058_03260"
     CDS             1012..2181
                     /locus_tag="VF058_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX6207355.1"
                     /translation="MSLDDDLVASLLATWRDLHQHPELAFQEARTAGIVAERLRAIGL
                     EDVREGVAETGVTATLRGGGPGPTLLLRAEMDALPISEDTGLPYASRNDGVMHACGHD
                     AHTAILLAVAEALARDRGELPGTVRFAFQPAEEIGAGAARMVEAGVMNDPPVAGVLAL
                     HMHAHIPTGVIGIPPRAAAASAAEIEIEIRGRGGHSAFPNELVDPVLTSAHVITALDA
                     LVAREVSPLRRAVLTIATVQAEGAFNIVPDTARLTGTIRAEDLELHERLAVRAEEITK
                     GIASAFRCEAELTHRPFCPPVMNHPDMVAIARAAAAEVAGADRVVDAPPVMAADDLAF
                     LTERAPGCYVFVGIAPTDGRPQVPHHNRRFAVDESSVQLGARALLVAARRFLERG"
     gene            2187..2876
                     /locus_tag="VF058_03265"
     CDS             2187..2876
                     /locus_tag="VF058_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207356.1"
                     /translation="MGPDRVPPRWTLAVVLTAGMMFGTFGAPAISVLAPFLVDELELT
                     RTQLGSLFAISAALGAVFAPLAGRRPAHAGAVASALDLTAVGSRILWSRVGEGLERFA
                     LSLGLISALSVMVVAAVWAAPAIGVWLLWAGAIGAGASPAAWGGFTMLAVIGGTEARH
                     AGRASGLIQLGFLAGLAWGPAAFGLIVDVTGSYAIGWAGVVLLLTAGGFVTLVERRSG
                     PVRLDVRQSRR"
     gene            complement(2861..3970)
                     /locus_tag="VF058_03270"
     CDS             complement(2861..3970)
                     /locus_tag="VF058_03270"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX6207357.1"
                     /translation="MIFFPEFHAFAFQTQRLMAEIPGGGADVMEIARAVERIQDADFE
                     SWRAGWEWIADHVRDLAERNGAEGHRVSAREGFFRAANYYRSSEFFLPQDDPRKLETW
                     KRMVTCFERAAALSDPPFEWFRSPYEEGDGLPGYLARPLAAEGPLPTVLYLNGADGTK
                     EESWYLAGKAFVDRGVNFVAIDGPGQGEVLRVQEVYSRPDYEAAISPLYDVLAARDDV
                     DAERMALVGISMGGYYAGRIACYEHRFRAYALHGACHSVLEDLYEPYEPIRRQVQWFT
                     GTFDDAKAREVLRDFDLGPHLGNVTRPTYICHGTEDVLVQPAAAQKTYDGITGAEKEL
                     RWWDREETGSLHANVDNPSEAYPALADWVVDHLRD"
     gene            complement(4004..>4328)
                     /locus_tag="VF058_03275"
     CDS             complement(4004..>4328)
                     /locus_tag="VF058_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX6207358.1"
                     /translation="DQAEFLNADETGPHTPGVDVECAKWLAEEAPIVGMGVETVGTDA
                     GAAHSFDPAFPCHHFLLGAGKYGLTQLANLDQLPPVGSVLLVAPLKLERGSGSPTRAL
                     ALVER"
BASE COUNT          590 a         1579 c         1517 g          642 t
ORIGIN      
        1 ccccgcgacc ccgacggtcg tcaagcagat cccccgcgac cccggcaccc actcgcacaa
       61 ggtggtcgtg ggcgacggcc tcatgctcgc caactgcgaa cgcgacctgg tgtgggaggg
      121 gccacccttc gtggagccca ccgggggcgc cgatgagtgg aacccgggac tccgcgttta
      181 cgacgcctcc gaccccgcgg atccgaagga gatcgccttc tatcaaaccg aggggaccgg
      241 cgtccaccgc atgaccttcg agccgcccta cgtctacatg agcgggagcg acgagggctt
      301 cacggagcag ttcctcatca tcctggacct ctcggacccc gcgaaacccg tcgaggccgg
      361 acggtggtgg tacccgggga tgtggacggg gggcggcgag cggcctcgat tcccccccga
      421 ccgccgctac gccctccacc acggcctgct cgacggcgac ctcgtcttct gcgggtggtg
      481 ggacgcgggg ctcgtgatcc tcgacgtggc cgatcggtcg acccctcagc tcgtggccaa
      541 cctcaactgg gggccgagcg agagcggggc cacgcacact gcgctccccc tgcacgggcg
      601 cgacctcgtg gtggtcacgg acgaggcgat cgtagacgag tgccgggacg tcacgaagca
      661 ggtgcgggtc ctcgacgtgt cggatcccta cacgccgaag gaggtgtcgc gcctacccat
      721 ccccgacggg aatttctgcg agcgaggcgg gcggttcggg ccgcataacc tcgccgagcc
      781 ccgccccttc accaaggtcg agaccgaccg ggtctacgtc gcgtacttca acgcggggct
      841 ccgcgtgatc gacctcgagg accccgagcg tccacgcgag atcgcccatc tcatcccgga
      901 tcccccaccg gggaagccca cctcgatggt ggacgacgtc gcagtcgccg acgacgggac
      961 gatctacctc accgatcggg cgggcggcgg gctgcacgtc gtcgggtggg cgtgagcctc
     1021 gacgacgacc tcgtcgcgtc gctgctcgcc acctggcgcg acctccacca gcatccggag
     1081 ctcgcgttcc aggaggcgcg cacggccggc atcgtcgcgg agcgactccg tgccatcgga
     1141 ctcgaggacg tccgggaggg cgtcgccgag accggcgtga cggccaccct ccgcgggggc
     1201 ggacccggcc ccaccctcct gctccgtgcg gagatggacg ccctcccgat cagcgaggac
     1261 acggggctgc cgtacgcgtc ccgcaacgac ggggttatgc acgcatgcgg ccacgacgcg
     1321 cacacggcga tcctcctggc ggtcgccgag gccctggccc gggatcgagg cgagctcccc
     1381 gggacggtgc gcttcgcctt ccagcccgcg gaagagatag gtgccggcgc ggctcggatg
     1441 gtggaggcgg gggtcatgaa tgacccaccc gtggccggag tgctcgcgct ccatatgcac
     1501 gcgcacatcc cgaccggcgt gatcgggatc cccccccgag cggccgcggc tagcgcggcg
     1561 gagatcgaga tcgagatccg tgggcgggga ggtcactcgg cgttccccaa cgagctggtg
     1621 gatcccgtcc tgacctcggc ccatgtgatc acggcactgg acgccctcgt cgcgcgcgag
     1681 gtctccccgc ttcggcgcgc ggtgctgacg atcgccaccg tgcaggccga gggcgcgttc
     1741 aacatcgtcc cggacaccgc ccgcctgacg gggacgatcc gcgccgagga cctggagctc
     1801 cacgagcgcc tcgcggtacg tgccgaggag atcaccaagg ggatcgcctc ggccttccgg
     1861 tgcgaggcgg agctgaccca ccgcccgttc tgtcccccgg tgatgaacca ccccgacatg
     1921 gtcgcgatcg cccgggccgc ggcggcggag gtggcggggg cggatcgggt ggtggacgct
     1981 ccccccgtga tggccgcgga cgatctcgcc ttcctcaccg agcgagcccc cggctgctac
     2041 gtgttcgtgg ggatcgcgcc caccgacggc cgtccccagg tgccgcacca caaccgccgc
     2101 ttcgccgtcg acgagtcctc ggtgcagctg ggagcccggg ccctcctggt ggccgcgcgg
     2161 cgcttcctcg agcgaggctg agcgcggtgg gccccgatcg cgtgcctccc cgctggacgc
     2221 tcgccgtcgt cctgacggcc ggcatgatgt tcggcacgtt cggcgcgccc gcgatctcgg
     2281 tcctcgcgcc gttcctcgtc gacgaactcg agctcacccg gacccagctg ggatccctct
     2341 tcgccatctc cgccgcgctc ggcgccgtct tcgccccgct cgcggggcgc cggccggccc
     2401 acgcgggggc cgtggcctcg gcgctggacc tcaccgcggt gggctcgagg atcctgtgga
     2461 gcagggtcgg cgaggggctg gagcggttcg cgctgtcgct cggtctgatc tcggcgctct
     2521 cggtgatggt ggtggccgcc gtgtgggcgg cccccgcgat cggggtgtgg ctgctctggg
     2581 cgggggcgat cggggccggc gcgtcgcccg ccgcctgggg cgggtttacg atgctggcgg
     2641 tgatcggggg aactgaggca cgacacgccg gccgggcctc ggggctgatc cagctcggct
     2701 tcctggcggg gctcgcgtgg ggccccgccg ccttcggcct catcgtggac gtcaccggga
     2761 gctacgcgat cggctgggcc ggcgtggtcc tcctgctgac ggccgggggc ttcgtaaccc
     2821 tcgtcgagcg gcggtcggga ccggtccgcc tcgatgtccg tcagtcgcgc aggtgatcca
     2881 ccacccagtc cgccagggcc gggtacgcct cgctcgggtt atcgacgttg gcgtggaggc
     2941 tccccgtctc ctcccgatcc caccaccgca gctccttctc ggcccccgtg atgccgtcgt
     3001 acgtcttctg tgcggcggcg ggctgcacga gcacgtcctc ggtcccgtgg cagatgtacg
     3061 tggggcgcgt cacgttcccc aggtgcggcc ccaggtcgaa gtccctcagc acctcgcgcg
     3121 ccttcgcgtc gtcgaaggtc ccggtgaacc actggacctg gcgtcggatc ggctcgtagg
     3181 gctcgtagag gtcctcgagg acgctgtggc acgctccatg gagggcgtag gcgcggaagc
     3241 ggtgctcgta gcaggcgatc cggcccgcgt agtacccgcc catgctgatc cccacaagcg
     3301 ccatgcgctc ggcgtccacg tcgtcacgtg ccgcaaggac gtcgtacagc ggtgagatcg
     3361 ccgcctcgta gtccggcctg ctgtacacct cctgcacgcg gaggacctcg ccctggccgg
     3421 gcccgtcgat cgccacgaag ttgacgcccc ggtccacgaa ggccttgccc gcgaggtacc
     3481 aggactcctc cttggtccca tcagcgccgt tgaggtagag gacggtcggg agcggcccct
     3541 cggctgccag aggacgggcc aggtacccgg gcaggccatc gccctcctcg tagggggagc
     3601 ggaaccactc gaagggcggg tcggagaggg ccgcggcccg ctcgaagcag gtcaccatcc
     3661 gcttccatgt ctcgagcttg cgagggtcgt cctggggcag gaagaactcc gaggaccgat
     3721 agtagttggc ggcgcggaag aacccctccc gggcggagac tcgatggccc tcggcgccgt
     3781 tccgctcggc gaggtcgcgc acgtgatcag cgatccactc ccacccggct cgccagctct
     3841 cgaaatcggc gtcctggatc cgctccacgg cgcgggcgat ctccatgacg tcggcgccac
     3901 cgccggggat ctcggccatc agtcgctggg tctggaaggc gaacgcgtgg aactccggga
     3961 agaagatcac gaggaacctc ccatccgcat catggcgtcg cgatcaccgc tccaccagcg
     4021 ccagcgcgcg ggtcgggctc cccgaccccc gctccagctt cagcggcgcc acgaggagca
     4081 cggaccccac gggcggaagc tggtcgaggt tggcgagctg ggtaagcccg tacttccccg
     4141 ccccgagcag gaagtggtga catgggaatg ccggatcgaa cgagtgggcc gcgccggcgt
     4201 cggtgcccac cgtctccacc cccatgccca cgatcggggc ctcctccgcc agccacttcg
     4261 cgcactcgac atccacgccg ggcgtgtggg gacccgtctc gtcggcgttc aggaactcgg
     4321 cttggtcg
//
LOCUS       DASUTW010000102         6730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_418143, whole genome shotgun sequence.
ACCESSION   DASUTW010000102 DASUTW010000000
VERSION     DASUTW010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418143"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1992)
                     /gene="topA"
                     /locus_tag="VF058_03280"
     CDS             complement(<1..1992)
                     /gene="topA"
                     /locus_tag="VF058_03280"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX6207359.1"
                     /translation="MATKLVIVESPTKAKTLERYLGPGYRVKASYGHVRDLPKSKMAV
                     DTERNFEPEYEVPDAKKKVVAELKRAHKQADELWLATDFDREGEAIAFHVAQLLGVSP
                     EEAKRVTFTEITESAVREAFAEPRTIDLQLVEAQQARRILDRLVGYSISPLLWKKVRP
                     GLSAGRVQSPALRLIVEREREIAAFQPKEYWSVDARLAPNGDEDAFLAGLWQIGEEKL
                     AANPDKKGLVLESEEVAREHAGNLDRAAYRVAEVRRSERKRNPSPPFTTSTLQQEAAR
                     KLGFTSRKTMRLAQQLYEGVELPGEGQVGLITYMRTDSVAIAAQALAEIAEVVRLQYG
                     KEYALDKPRRYKKKQRGAQEAHEAIRPTSAARLPDVVQAHLDRDQARLYRLIWQRTMA
                     SQMAPAVFDQVSVDIEAVADDAPRYLLRATGQTMKFDGFRRAYFEGRDDDVDEDAESR
                     LPELTAEQALRLLEVLPEQHFTQPPPRYTEASLIKKLEELGIGRPSTYSSIVSTLLDR
                     EYVRLEERRFYPEDVGEVTTDVLVDRFGDHFLDYDFTARMEGELDDIAEGKMRWNQVL
                     DEFHAPFERKVEKAEEELERPEEELDELCPRCPEEGREPGKLVIKLGRRGKFVGCKNY
                     PECRYTRDVDGQERPEPELLEETCPECGKQLVKRWGRYGP"
     gene            2100..2759
                     /locus_tag="VF058_03285"
     CDS             2100..2759
                     /locus_tag="VF058_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX6207360.1"
                     /translation="MLEWLEHLIVDVIGTYGYFAIFVLMMLESALIPIPSEVTMVFGG
                     FLVARGDLDFIAVGTIGAVANVVGSWLAYYVGRVGGRPLAERWGRYVFLREKELDRAD
                     EWFANRGELTVFVTRLLPVIRTFISFPAGVARMPFWRFTLYTFLGCLPWSFALAWAGV
                     LLGENWEVFLRHSRPVVIAVGVATAALIGWWLWRRAAARRQEREERDEPEPAEEDELT
                     R"
     gene            complement(2749..3300)
                     /locus_tag="VF058_03290"
     CDS             complement(2749..3300)
                     /locus_tag="VF058_03290"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX6207361.1"
                     /translation="MERADAVALVREKTEKETTVRHLISVEGVMRALARRFGEDEETW
                     GLVGLFHDLDQDQTHDDLERHAYRAAEWLREAGVDERIVTGVLAHAHEEHRTDRMTRA
                     IVHADALAGLLVASALVRPQRAEGMKVSSVRKKLKEKAFAPGVNREEITSVEESIGLP
                     LDDFIAAGIEGLQEVAPQIELTG"
     gene            complement(3287..5623)
                     /locus_tag="VF058_03295"
     CDS             complement(3287..5623)
                     /locus_tag="VF058_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX6207362.1"
                     /translation="MPRIGEVVKRVLVGRAHATHMAEHQLLPKSLALPVFSSDPLSSV
                     AYATEEIMLVLVLAGAMALRFTVPIALGITLVLAIVITSYRQTVRAYPRGGGSYIVAR
                     ENLGTIPGLIAASAILIGYVVTAAVSVTAGSIAVTSAVPELVEQRVPIAIGFLVVIAL
                     ANLRGVRESGMVFAVPTYGFVLTIYVTLLVGFARCLGGCPVAATADLAIEPHEALTAL
                     VLLRAFAQGSAALTGVEAIADGVQAFRRPKGRNAAATMAIMGSMGISMFVGITVLARL
                     VDIRVSEDVVVSSSVLSQIGETVFGRGGMFFVLQAFTAMILIVAANTAYQDFPRLSAI
                     LARDRFMPRQFMNRGDRLVYSNGIVVLTVAASLLVWAFDATLTRLIPLYLVGVFTAFT
                     LSQAGMVRRWLRLKEGPWLRNAIINGSGASATGLVLAVAVTTRFVEGSWMVVAAIPVI
                     IAGLLSVHRHYERVRRELRSRDLSPSMEAENTMLVLVPRVDRATREMVSWLLAVRPSR
                     ITPLYIGSDDFEEAAGAWAVAAPRLPPLQPLEVRNGQVTKGLRRYIRRLPRGENSFVT
                     VVLPEEVTTGFWLYVARRALTFRIKTALLFEPGVVVTDVPLLPEEAEKEETWAAGERA
                     LEPERNVCLVPVSQVHDGTIRAIVWARSIRPAHLEAVFLAEEPEEADELLAAWREAGI
                     DVPLSIVEAPFRDYGPPLLEEVRRHSKRGDTVVTVVFPEFVVDRWWQQLLHNQNSFFM
                     KRLLLFEPWVVGVSVPFHLGRPPIEAEKPEGAKTDGAS"
     gene            5681..6334
                     /locus_tag="VF058_03300"
     CDS             5681..6334
                     /locus_tag="VF058_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX6207363.1"
                     /translation="MHMVIMGCGRVGAELTVQLSKMGHSVSVIDKRPEAFDRLPPGFD
                     AKKIVGIGFDQEVLEEAGIKEADAFIAVSNGDNSNILSARIAREHYKVPRVIARIYDP
                     RRAEIYERLNIPTVATVRWAAKQIQFLLFHGKEELRDTIAGGSLLVVQVPIPDHLVGQ
                     KIEEIRVEGKIEVIAVDRGGAGFIPVPGSTFQEGDVAHAVVHRESLDEFEALLEVTE"
     gene            6334..>6730
                     /locus_tag="VF058_03305"
     CDS             6334..>6730
                     /locus_tag="VF058_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX6207364.1"
                     /translation="MRVTIAGAGNVGRHLAYDLAQRGHDVTIIEQETSILERAKDALP
                     AGTMFVLGDACEPWVLEKAELSSADVIVAATGDDEDNLVISLLAKQEHGVPRVVARVN
                     HPKNEWLFTEQWGVDVSMSPPHVLTALVEE"
BASE COUNT         1014 a         2318 c         2361 g         1037 t
ORIGIN      
        1 cggcccgtac cgcccccacc gcttcacgag ctgcttgccg cactcggggc aggtctcctc
       61 aagcagctcg ggctcgggtc gctcctggcc gtccacgtct cgcgtgtagc ggcactcggg
      121 gtagttcttg cagcccacga acttgccccg ccgccccagc ttgatcacga gcttcccggg
      181 ctcacgcccc tcctcgggac agcgggggca gagctcgtcg agctcctcct ccggccgctc
      241 cagctcctcc tcggccttct ccaccttccg ctcgaacggg gcgtggaact cgtcgagcac
      301 ctggttccag cgcatcttgc cctcggcgat gtcgtcgagc tcgccctcca tcctggcggt
      361 gaagtcgtag tcgaggaagt gatccccgaa ccggtccacg agcacgtcgg tcgtgacctc
      421 gcccacgtcc tcggggtaga agcggcgctc ctcgagccgc acgtactcgc ggtcgagcag
      481 ggtggagacg atcgaggagt aggtggacgg gcggccgatc cccagctcct cgagcttctt
      541 gatcagcgag gcctccgtgt agcggggcgg cggctgggtg aagtgctgct cggggaggac
      601 ctccaggagg cggagggcct gctcggcggt gagctcgggg aggcgcgact ccgcgtcctc
      661 gtccacgtcg tcgtcgcggc cctcgaagta ggcccggcgg aagccgtcga acttcatcgt
      721 ctgcccggtg gcgcggagga ggtaccgcgg cgcgtcgtcg gcaaccgcct cgatgtccac
      781 cgacacctga tcgaagacgg ccggggccat ctggctggcc atcgtccgct gccagatcag
      841 ccggtagaga cgggcctggt cccggtcgag gtgggcctgg acgacgtcgg ggaggcgggc
      901 ggcagaggtg gggcggatcg cctcgtgcgc ctcctgcgcc ccgcgctgct tcttcttgta
      961 gcggcggggc ttgtccagcg cgtactcctt cccgtactgg aggcggacga cctcggcgat
     1021 ctcggcaagg gcctgggcgg cgatggccac cgagtcggtc cgcatgtagg tgatgagccc
     1081 gacctgcccc tcgccgggga gctcgacgcc ctcgtacagc tgctgggcca ggcgcatcgt
     1141 cttcctcgac gtgaacccga gcttgcgggc ggcctcctgc tggagggtcg aggtggtgaa
     1201 ggggggcgag gggttcctct tccgctcgga gcgccgaacc tcggccacgc ggtaggccgc
     1261 gcggtcgagg ttgcccgcgt gctcccgggc cacctcctcg ctctcgagga ccagcccctt
     1321 cttgtcgggg ttggccgcga gcttctcctc gccgatctgc cacaggccgg cgaggaacgc
     1381 gtcctcgtcg ccgttcggcg cgagccgggc gtccacgctc cagtactcct tgggctggaa
     1441 ggccgcgatc tcacgctcgc gctccacgat gagccgcagc gccggcgact gcacccggcc
     1501 agccgacagg cccggccgca ccttcttcca caggagcggg gagatcgagt agccgacgag
     1561 ccggtcgaga atgcgacggg cctgctgagc ctcgacgagc tggaggtcga tcgtccgtgg
     1621 ctcggcgaag gcctcccgca cggccgactc ggtgatctcg gtgaaggtga cgcgcttcgc
     1681 ctcctcgggg ctgaccccga gcagctgggc cacgtggaag gcgatcgcct cgccctcgcg
     1741 gtcgaagtcg gtggccagcc agagctcgtc ggcctgcttg tgggcgcgct tcagctccgc
     1801 gaccaccttc ttcttggcgt cgggcacctc gtactccggc tcgaagttgc gctcggtgtc
     1861 gacggccatc ttgctcttgg ggaggtcgcg gacgtgcccg taggaggcct tgacccggta
     1921 gcccgggccg aggtaccgct ccagggtctt ggccttggtg ggcgattcga cgatgacgag
     1981 tttggttgcc acgagcccgc tttcatacac gagggaagga tcggcgtcaa cgagacgcca
     2041 cgcgcgagga tagcgcgtcg gaccgtccag ataacgcaac tgctaggctg gccattccca
     2101 tgctggagtg gctcgaacac ctgatcgtcg acgtgatcgg cacgtacggc tacttcgcca
     2161 tcttcgtcct gatgatgctg gagtcggccc tgatcccgat cccctccgag gtcacgatgg
     2221 tgttcggcgg gttcctcgtg gcccgagggg acctcgactt catcgcggtg gggacgatcg
     2281 gcgcagtggc caacgtggtg gggtcctggc tcgcctacta cgtggggcgc gtcggggggc
     2341 ggcccctggc cgagcgctgg ggtcgctacg tcttccttcg ggagaaggag ctcgatcgag
     2401 cggacgagtg gttcgcgaac cgcggggagc tcacggtctt cgtcacgcgc ctgctccccg
     2461 tgatccggac gttcatcagc ttcccggccg gcgtggcccg gatgccgttc tggcggttca
     2521 cgctctacac cttcctgggc tgcctcccgt ggtcgttcgc tctcgcgtgg gccggcgtgc
     2581 tgctgggcga gaactgggag gtgttcctgc gccactcgag gccggtggtg atcgccgtgg
     2641 gcgtggcgac ggcggctctg atcgggtggt ggctgtggcg ccgggccgcg gcccggagac
     2701 aggagcgcga ggagcgcgac gagccggagc ccgcggagga ggacgagctc acccggtgag
     2761 ctcgatctgg ggggccacct cctggagccc ctcgatcccg gcggcgatga agtcatccag
     2821 cgggagaccg atgctctcct ccaccgaggt gatctcctcg cggttcacgc cgggggcgaa
     2881 ggccttctcc ttcagcttct tcctcaccga ggagaccttc atgccctcgg ccctctgggg
     2941 tcggacgagc gccgaggcca cgagcagccc cgctaaggcg tccgcgtgca cgatggcccg
     3001 ggtcatccgg tcggtgcggt gctcctcgtg ggcgtgggcc agcacgccgg tcacgatccg
     3061 ctcgtccacc ccggcctccc ggagccactc ggccgcccgg taggcgtggc gctccaggtc
     3121 gtcgtgggtc tggtcttggt cgaggtcgtg gaagagcccg acgagccccc aggtctcctc
     3181 gtcctcgccg aagcgccggg ccagggctcg catcacccct tccacgctga tcaggtgccg
     3241 gacggtcgtc tccttctctg tcttctcccg caccagcgcg accgcgtcag ctcgctccat
     3301 ccgtcttcgc tccctccggc ttctcggcct cgatcggcgg cctccccagg tggaagggca
     3361 cgctgacgcc gaccacccac ggctcgaaca gaagcagccg cttcatgaag aaggagttct
     3421 ggttgtggag cagctgctgc caccatcgat ccaccacgaa ctcggggaag acgaccgtca
     3481 cgacggtgtc gcctcgtttg ctgtggcggc ggacctcctc caggagcggc gggccgtagt
     3541 cgcggaacgg ggcctccacg atcgagagcg gcacgtcgat ccccgcctcc cgccaggccg
     3601 ccagcagctc gtccgcctcc tcgggctcct cggccaggaa caccgcctcc aggtgtgcgg
     3661 ggcggatcga gcgggcccag acgatggcgc ggatcgtccc gtcgtggacc tgggacacag
     3721 ggacaaggca gacgttgcgc tcgggctcga gggcccgctc gccggccgcc cacgtctcct
     3781 ccttctcggc ctcctcgggg agcaggggca cgtcggtcac gacgaccccc ggctcgaaca
     3841 gcagcgcggt cttgatccgg aaggtcaggg cccggcgcgc gacgtacagc cagaagcccg
     3901 tcgtcacctc ctcgggcagg accaccgtca cgaagctgtt ctcgccccgg gggaggcggc
     3961 ggatgtaccg gcgcaggccc ttcgtgacct gcccgttacg gacctccaga ggttggagcg
     4021 gagggaggcg aggggcggcc acggcccacg ctcccgccgc ctcctcgaag tcgtccgatc
     4081 cgatgtagag gggagtgatg cgggagggcc ggacggcgag cagccaggag accatctccc
     4141 gcgtggcccg gtccacccgg ggaaccagga ccagcatggt gttctcggcc tccatcgagg
     4201 gcgagaggtc ccgcgagcgc agctcacgcc gcacccgctc gtagtggcga tgcacggaca
     4261 ggagccccgc gatgatgacg gggatcgcgg ccacgaccat ccacgagccc tcgacgaagc
     4321 gggtcgtgac ggccaccgcg agcaccagtc ctgtggccga cgcgccggac ccgttgatga
     4381 tcgcgttccg gagccagggg ccctccttca ggcgcagcca ccgccgcacc atgcccgcct
     4441 gcgacagggt gaaggccgtg aagacgccga cgaggtagag cgggatcagg cgggtcagag
     4501 tcgcgtcgaa cgcccacacg agcagggacg cggcgaccgt gagcaccacg atcccgttcg
     4561 aatacacgag gcggtcgccg cggttcatga actggcgggg catgaagcgg tcccgagcca
     4621 ggatcgccga caggcgcggg aagtcctggt aggcggtgtt ggccgccacg atgaggatca
     4681 tggccgtgaa ggcctgcagg acgaagaaca tccccccgcg tccgaagacc gtctccccga
     4741 tctgggacag caccgagctc gacaccacga catcctcgga caccctgatg tccacgagcc
     4801 gggccagcac cgtgatgccc acgaacatcg agatccccat cgagcccatg atcgccatgg
     4861 tggcggccgc gttcctcccc ttgggccggc ggaaggcctg caccccgtcc gcgatcgcct
     4921 cgacgccggt cagggccgcg gagccctggg cgaaggcgcg gagcaggacg agcgcggtga
     4981 gcgcctcgtg gggctcgatc gcgaggtcgg ccgtggcggc gaccgggcac cccccgagac
     5041 agcgagcgaa ccccaccagg agcgtgacgt agatcgtcag cacgaagccg tacgtgggga
     5101 cggcgaagac catccccgac tcacgaacgc cccgcaggtt cgccagagcg atgaccacca
     5161 ggaacccgat cgcgatcggg acccgctgct cgaccagctc gggcacggcg gaggtcaccg
     5221 cgatcgaccc ggcggtgaca ctgaccgcgg cggtcacgac gtagccgatc aggatggccg
     5281 aggcggcgat caggccgggg atcgtcccca agttctcgcg ggccacgatg taggagccgc
     5341 cccccctcgg ataggcccgc acggtctggc gatagctcgt gatgacgatc gccagcacga
     5401 gcgtgatccc gagggcgatg gggaccgtga accgcagcgc catcgccccc gccaggacga
     5461 ggacgagcat gatctcctcg gtggcgtagg cgaccgacga gagggggtcc gacgagaaga
     5521 ccggcagggc caggctcttg ggaaggagct gatgctcggc catgtgcgtg gcgtgggccc
     5581 ggcccacgag cacccgcttg acgacctctc cgatccgtgg cacgagccgg attctacgcc
     5641 cgcgtttcca gccggagcgg accgcggtat cgttcgcgtc atgcacatgg tcatcatggg
     5701 atgcgggcgc gtgggagccg agctcacggt ccagctctcg aagatggggc actcggtctc
     5761 cgtcatcgac aagcgtccgg aggcgttcga ccgactgccg cccggcttcg acgcgaagaa
     5821 gatcgtgggc atcggcttcg accaggaggt cctggaggag gcgggcatca aggaggcaga
     5881 cgccttcatc gctgtctcca acggcgacaa ctcgaacatc ctctcggccc gcatcgcccg
     5941 cgagcactac aaggtcccgc gggtcatcgc ccgcatctac gacccgcgtc gagccgagat
     6001 ctacgagcgg ctgaacatcc ccacggtggc caccgtgcgc tgggcggcga agcagatcca
     6061 gttcctcctc ttccacggga aggaggagct gcgcgacacg atcgccgggg gctcgctgct
     6121 ggtggtccag gtcccgatcc cggaccacct cgtggggcag aagatcgagg agatcagggt
     6181 cgaggggaag atcgaggtga tcgccgtgga tcgcggcggg gccggcttca tccccgtgcc
     6241 cggctcgacc ttccaggagg gagacgtggc gcacgcggtc gtgcatcggg agtctctcga
     6301 cgagttcgag gccctcctgg aggtgacgga gtgatgcgcg tgacgatcgc gggcgccggg
     6361 aacgtggggc gccacctcgc ctacgacctg gcccagcgcg gccacgacgt gacgatcatc
     6421 gagcaggaga cctcgatcct cgagcgggcc aaggacgccc tccccgcggg gacgatgttc
     6481 gtcctgggag acgcctgcga gccctgggtg ctggagaagg ccgagctctc gtcggccgac
     6541 gtcatcgtgg ccgccaccgg ggacgacgag gacaacctcg tcatctccct gctggccaag
     6601 caggagcacg gtgtgccgag ggtggtggcc cgggtcaacc atcccaagaa cgagtggctg
     6661 ttcaccgagc agtggggcgt ggacgtctcg atgagccccc ctcacgtcct gacagccctg
     6721 gtcgaggagg
//
LOCUS       DASUTW010000103         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_418258, whole genome shotgun sequence.
ACCESSION   DASUTW010000103 DASUTW010000000
VERSION     DASUTW010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418258"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1335)
                     /gene="aroA"
                     /locus_tag="VF058_03310"
     CDS             complement(25..1335)
                     /gene="aroA"
                     /locus_tag="VF058_03310"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX6207365.1"
                     /translation="MRLVVRPSGPLRGTVAVPGDKSIAHRWLLLAATAEGGSELDGLP
                     QADDVRSTAACVLRLRGNQPGLEAWLRGEPGPVRLEGRGWDGLAPPAWPLDCGNSGTT
                     MRLLAGVLAGRDFSCVLEGDESLAARPVERVAEPLRLMGAEIHSEDGRPPLQVLGGGL
                     KGIEYRLPVPSAQVQGAVLLAGVQAVGETVVDGSVFDPGSIRDHSERALAALGAPVET
                     EGGRVAVRAFQHPGLRGAVPGDPSAAAFLLAGAAILPGSEVEIRDVGINPTRLAFASL
                     IGATVDEGRREELGEPVGTLARQGADPAPIEVPAAGLPAVVDEVPALAAVAVHAGGES
                     WFRGAGELRVKESDRLRGLALGLRGLGGEAEVQGDDLVVGGDGLRGGEADSQGDHRLA
                     MALTVAALAAERESVIHDVQWAAVSFPGFVDALRALGADVEVEG"
     gene            complement(1335..>2084)
                     /locus_tag="VF058_03315"
     CDS             complement(1335..>2084)
                     /locus_tag="VF058_03315"
                     /inference="COORDINATES: protein motif:HMM:NF042869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX6207366.1"
                     /translation="GRFVGGHPMTGSERSGPAGAAAGLIDGAAWALTPTDRTDPAALA
                     SVEDAVRRMGAVPVTLSPDRHDRLVGLVSHLPQVASIALMSTVASSADETALLLAAGG
                     FRDLTRLAASSPELWVDILLSNREAVAEAIDGYGERLRSLAGMLQAGDAVGLTETFAR
                     AKEARLGLGARPQSRVGLAILAVPIPDRPGSLAEITGALADRGVNIEDLEIVHRAEGA
                     RGTAHVTVGEADADAAVVALTSSGHEAARLA"
BASE COUNT          270 a          808 c          781 g          225 t
ORIGIN      
        1 cgtcgatcgc caccacgggg ccgctcaccc ttccacctcc acgtcggccc cgagcgcgcg
       61 gagggcgtcc acgaagcccg ggaacgagac ggcggcccac tggacgtcgt ggatcacgct
      121 ctcgcgctcg gcggcgaggg ccgccacggt cagggccatc gccagccggt ggtcgccctg
      181 ggagtcggcc tccccgccgc ggagcccatc accacccacc acgaggtcgt cgccctggac
      241 ctcggcctcg ccgcccaggc cgcggaggcc cagggccagg cctcggaggc ggtcggactc
      301 cttcactcgg agctcgccgg cgccccggaa ccacgactcc cccccggcgt gcacggcgac
      361 agccgccagg gcagggacct cgtccacgac ggccgggagt ccggcggccg ggacctcgat
      421 cggcgccgga tcggcgccct gccgggccag cgtgccgacc ggctcgccga gctcctcgcg
      481 ccgcccctcg tcgacggtcg ctccgatgag tgaggcgaac gcgagccggg tggggttgat
      541 cccgacgtcc cggatctcga cctcggagcc gggcaggatc gcggcgccgg ccagcaggaa
      601 ggcggccgcg gagggatcgc ccgggacggc gccccgcagg cccggatgct ggaacgcgcg
      661 gaccgcgacc cgtcctcctt ccgtctcgac cggggcgccc agcgccgcca gggcccgctc
      721 ggagtggtcg cggatcgagc cgggatcgaa gacggagccg tccaccaccg tctcgcccac
      781 ggcctggacc ccggccagca ggaccgcgcc ctgcacctgc gcgctcggaa cgggaagccg
      841 gtactcgatc cctttgagac cgccccccag cacctggagc ggcgggcggc cgtcctcgct
      901 gtggatctcg gctcccatga ggcgcagcgg ctcggccacg cgctccacgg ggcgggcggc
      961 gaggctctcg tcgccttcga ggacgcacga gaagtcgcgg ccggcgagga cccctgccag
     1021 gagccgcatc gtcgtcccgg agttgccgca gtcgagcggc caggcgggcg gagcgaggcc
     1081 gtcccagccc cggccctcga gccgcacggg accgggctcg ccgcggagcc aggcctcgag
     1141 cccgggctgg ttccccctca gccggaggac gcaggcggcg gtcgagcgga cgtcgtcggc
     1201 ctggggcagg ccgtcgagct cggagccgcc ctccgcggtc gcggccagca ggagccagcg
     1261 gtgggcgatc gacttgtctc cggggacggc gacggtccct cgcaggggcc ccgagggtcg
     1321 gacgacgagg cgcatcaggc caggcgggcg gcctcgtggc cgctcgaggt cagcgccacg
     1381 acggcggcgt ccgcgtcggc ctcccccacg gtgacgtgcg ctgtgccccg ggcgccctcg
     1441 gccctgtgca cgatctcgag gtcctcgatg ttgacccccc gatcggcgag agcgccggtg
     1501 atctcggcca gcgagcccgg ccggtccggg atggggacgg ccaggatcgc cagccccacc
     1561 cgggactggg gtcgagcccc gaggcccaga cgggcctcct tggcccgagc gaacgtttcc
     1621 gtgagcccca cggcgtcgcc cgcctgcagc atgcccgcca ggctccgcag gcgctccccg
     1681 tagccgtcga tcgcctcggc caccgcctcg cggttggaca ggaggatgtc cacccacagc
     1741 tccgggctgc tcgcggccag acgagtgagg tctcggaagc cgccggcggc gagcaggagg
     1801 gcggtctcgt cggccgagga agccaccgtg gacatcagcg cgatcgaggc cacctgagga
     1861 aggtggctca cgaggccgac gagacgatcg tgacgatcgg ggctgagcgt cacggggacg
     1921 gcccccatcc ggcggacggc gtcctccacg gaggcgaggg ccgcgggatc cgtccgatcc
     1981 gtcggcgtga gggcccaggc ggcgccgtcg atcaggccgg cggcggcgcc ggcgggcccc
     2041 gaccgctccg agccggtcat cgggtggccg cccacgaacc ggcc
//
LOCUS       DASUTW010000104         7482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_30013, whole genome shotgun sequence.
ACCESSION   DASUTW010000104 DASUTW010000000
VERSION     DASUTW010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30013"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..508)
                     /locus_tag="VF058_03320"
     CDS             complement(26..508)
                     /locus_tag="VF058_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019813420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6207367.1"
                     /translation="MTTITSSIEVDVPLSTAYNQWTQFEEFPQFMEGVERVEQLDDTM
                     LRWTASIAGQERTWEAKILTQEPDRQVSWAAVGDVVHAGTVTFHELSPVRTRVDVEME
                     YEPQDWLEKAGDALNMIERRVDGDLERFKEFIESRGVESGAWRGRVEGGEETSGGPTV
                     "
     gene            604..1392
                     /locus_tag="VF058_03325"
     CDS             604..1392
                     /locus_tag="VF058_03325"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX6207368.1"
                     /translation="MLGAIPYRSLPQIPLGPISLKTFGLMVALGVLAGVALAARHARS
                     RGHSGEEVTRLAVVMVVTGFVGARVAWVLSHLDQIESPLDVVAVWEGGLAFTGGFVAA
                     TAVAVLALRRWPRTKRWEVADGMALGLTIGLAIGRLGCLAVGEHLGGPTDFFLGMRYE
                     GGATIEGPLEVGVVYHSTAIYEALHLLVLAVLLWWLLRPGRARPGTAITVFALWYGTA
                     RFLTDFLRAYDREVLGLTGAQWACLALLVAGAWAALRLRRPAPA"
     gene            1553..2572
                     /locus_tag="VF058_03330"
     CDS             1553..2572
                     /locus_tag="VF058_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX6207369.1"
                     /translation="MRIRTNLAVAAVALALLAAACGGEADAEGSGGLTGEIFVSGSST
                     VEPISGLNAEKFAEENPGVAISVEGPGTGDGFELFCGGETDISDASRPIAEDEAELCA
                     QNGIEYTELEVAIDGIAVLTSPQNEAVSCVGFGDVYALVGPESEGFDSWADAQGLAAE
                     ISSGEMPTPAADAGLPDAPLEITAPGEESGTYDSFIELTFEGFAEDERGQDPVTRPDY
                     VASADDNVIIEGIAGSPSSFGWVGFAFYRNNQDAVKALEVDGGSGCVAPSEETIADGS
                     YPVSRSLYIYVNDARAAESAAPRAFVDFYVSDEGLASVAEVGYVELPPERIEATRAAW
                     EEGRP"
     gene            complement(2569..3549)
                     /locus_tag="VF058_03335"
     CDS             complement(2569..3549)
                     /locus_tag="VF058_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX6207370.1"
                     /translation="MPLALVIFLTLLFQFANGWTDAPNAIATVVSTRVLRPYAAVVMA
                     AILNLVGALVTGTLVAETIAQGIVSPDVVTLETIGAAVFAAALWAMGAQVFGMPSSET
                     HALVASLLGAGFAAGGLNALEWSGTQKVVYSLFTSPVIAFFGGLLLMVGIYWAFRRAR
                     PRTVRRTFGKGQILSAAFMAFSHGSNDGQKGAGIIALALFLNGDLPELEIPLWVLFVV
                     ATTIGVGTAVGGWRVIRTIGLRMTKLEPVNGFAAETSAALIILGGSRLGIPLSTTHAI
                     GASIMGVGATRRLSAVRWGLAGEVVGTWILTWPACMLLSFAMAKLFQAIF"
     gene            complement(3550..4167)
                     /locus_tag="VF058_03340"
     CDS             complement(3550..4167)
                     /locus_tag="VF058_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX6207371.1"
                     /translation="MKFRLIPREESFYDLFIKQAEILVQATEVFRGIVTEFTDVKRRA
                     RRVHDLEHEGDEVTHEVMRRLNTTFVTPIDREDVHELASNMDDVLDHMDAAIDFLVLH
                     NIDRPIQEMKAQIDLLSEAAKATLEAMQTLPAFDRLSRYWIEINRLENEADRVYRQGV
                     ADLFSGEFEAMEVLRWKDIIDQLEKAMDKLEDVANTLEGIALKQA"
     gene            4313..5263
                     /gene="pstC"
                     /locus_tag="VF058_03345"
     CDS             4313..5263
                     /gene="pstC"
                     /locus_tag="VF058_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX6207372.1"
                     /translation="MRLPGGSGAAERIEGLRGDPRRRRRERSVGSVFFAAAALSVVVS
                     AAIVLSLAGEAASFLSKVDPSSLWSEGWFPRRSLYDLRTIVGGTIMVAGVAMVVAAPL
                     GLFTAMYLSELASPRARRWLKPVVEVLAGIPSVVLGYVALAFINPELVQRFLGGTSPF
                     SMLGAGVAVGILVTPLVASVAEDALHAVPQALREASSGLGAKRTTTTVRVVFPAAVSG
                     IVAALILGLSRAIGETMIVAIASGSTGGSLLSFDIVGPGQTMTGAMTALAVGSDRVAV
                     GGGGATVNPVDALFFVGLLLFVMTFALNVVSERLVRRMRV"
     gene            5263..6204
                     /gene="pstA"
                     /locus_tag="VF058_03350"
     CDS             5263..6204
                     /gene="pstA"
                     /locus_tag="VF058_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005580195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX6207373.1"
                     /translation="MGAARTLGATPARVATQDALRGTRMDLGGLLLHGLLLGSLLLTL
                     GVLATLLLQVGTTAWPVFADRGLDVVTSNLSSIASRAGVWQGIQGSMWLMAFVVLFSF
                     PLGIGAAIYIEEYARGTRLTRFINANIRNLAGVPSVVYGLLGLAIFVKLLGGVTGGRS
                     LISGGMTLAVLVLPIVIITASEALRAVPGGIREAAYGVGATRWETIRSHVLPYAAPGI
                     LTGTILALARAFGETAPLLMVGVATGFLSLGGGGLVDQLQGPYTALPAIIFRWSKLPQ
                     ADFREGLAAAAIVVLLVVLLAVNALAIILRNRYERRW"
     gene            6245..7036
                     /gene="pstB"
                     /locus_tag="VF058_03355"
     CDS             6245..7036
                     /gene="pstB"
                     /locus_tag="VF058_03355"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017615596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX6207374.1"
                     /translation="MEVRSAGEAILDLRNVSVSYGDVRAVEDITLSIREHEITALIGP
                     SGCGKTTVLRSLNRMNDLIEGARVDGRILYHGVDLYDERVDPVEVRRRIGMVFQKPNP
                     FPKSVFDNVAFGPRISGFEGDLEERVRWALTKAALWDEVKDTLRGPAVALSGGQQQRL
                     CIARAIAVEPDVILMDEPCSSLDPIATLKIEDLMRELVSDYTIVIVTHNMQQAARVSD
                     RTAFLTIDLEGGSRAGRLVEFDDTEVIFTNPSDPRTEDYVTGRFG"
     gene            7033..>7482
                     /locus_tag="VF058_03360"
     CDS             7033..>7482
                     /locus_tag="VF058_03360"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HEX6207375.1"
                     /translation="MSAEPRRRFHDELAGLEAAIGEMGERAERAVGGAMAALATGGVA
                     DAVVEADDAIDDRYLDVERRILDLLARQTPVASDLRLVSAILHINLHLERVGDIAVNI
                     AKIANATRDLPRSEPILAALVEMGDVVRPMLRASMEAFARRDLDLARR"
BASE COUNT         1011 a         2565 c         2725 g         1181 t
ORIGIN      
        1 ggacggcggc gcctcgcccg cgggctcaga cggtgggccc gccgctcgtc tcctcgccgc
       61 cctcgacgcg gcctcgccac gcgccggact ccacgccgcg gctctcgatg aactccttga
      121 accgctcgag gtcgccgtcc acgcgacgct cgatcatgtt cagcgcgtcg ccggccttct
      181 ccagccagtc ctggggctcg tactccatct ccacgtcgac ccgcgtccgt acgggcgaga
      241 gctcgtggaa ggtcaccgtc ccggcgtgga cgacgtcgcc cacggcggcc caggacacct
      301 ggcggtcggg ctcctgggtg aggatcttcg cctcccacgt gcgctcctga cccgcgatgc
      361 tggccgtcca gcgcagcatc gtgtcgtcga gctgctcgac gcgctcgacc ccctccatga
      421 actgcgggaa ctcctcgaac tgggtccact ggttgtaggc ggtcgacaga gggacgtcga
      481 cctcgatcga gctcgtgatg gtcgtcatcg gcacccacct ccttgtcctt ccctggatac
      541 ccgaggccgg tggtcggaaa ccggagcagg ggcggctacc ctggaggccg aaagggggga
      601 gatgtgctgg gggcgatccc gtaccggtct ctgccgcaga tcccgttggg ccccatctcg
      661 ctgaagacgt tcgggctgat ggtcgccctg ggcgtgctcg ccggagtggc gctggccgcc
      721 cgccacgccc gcagccgcgg ccactcgggc gaggaggtga ccaggctggc cgtggtgatg
      781 gtcgtcacgg gcttcgtggg ggccagggtg gcctgggtgc tctcccatct cgatcagatc
      841 gagtccccgc ttgacgtcgt ggcggtctgg gagggaggcc tcgccttcac cggtgggttc
      901 gtggcggcca ccgccgtggc cgtgctcgcc cttcgccggt ggccccggac gaagcgctgg
      961 gaggtcgccg acgggatggc gctcggcctg acgatcgggc tggccatcgg ccgcctgggg
     1021 tgcctggccg tgggcgagca cctgggcggc ccgacggact tcttcctggg gatgcgttac
     1081 gagggaggcg cgacgatcga ggggccgctg gaggtggggg tcgtctacca cagcaccgcc
     1141 atatacgaag cgctccatct tctggtgctg gccgtcctcc tctggtggct gctgcgtccg
     1201 gggcgggctc gccccgggac ggcgatcacg gtcttcgcgc tctggtacgg gaccgcgcgg
     1261 ttcctcaccg acttcctccg ggcctacgac cgggaggtgc tcgggctcac cggcgcccag
     1321 tgggcctgtc tggcgctcct cgtcgcggga gcgtgggccg ccctccgcct gcgccggccc
     1381 gcccccgcct gacctttcac ctcgcgttca tcccttcgtc gaggtcctgt cgacggcggc
     1441 tcctaggctc ggtcgcgtgg agagaggatg gcgagcggcc ggcggccgcc cggacagcgc
     1501 gcggaggaag cggacggcct ctccacccgc gcgcgcttga ggagggatct gcgtgcgcat
     1561 tcggacgaac ctggcggtcg cggcggtggc cctggcgctc cttgcggccg cgtgcggcgg
     1621 ggaggcggac gccgagggga gcggcggcct gaccggggag atcttcgtct cgggctcctc
     1681 caccgtggag ccgatctcgg ggctgaacgc cgagaagttc gccgaagaga acccgggcgt
     1741 ggcgatcagc gtggaggggc ccgggaccgg ggacggcttc gagctgttct gcggcggcga
     1801 gaccgacatc tccgacgcct cgaggccgat cgcggaggac gaggccgagc tctgcgcgca
     1861 gaacgggatc gagtacaccg agctcgaggt ggcgatcgac ggcatcgccg tcctgacctc
     1921 ccctcagaac gaggccgtct cctgcgtggg cttcggcgat gtctacgcgc tcgtcgggcc
     1981 ggagtccgag gggttcgact cgtgggccga cgcccagggg ctcgccgcgg agatctcctc
     2041 gggcgagatg ccgaccccgg ccgccgatgc tgggctcccg gacgcgccgc tggagatcac
     2101 ggcacccggt gaggagtccg ggacctacga ctcgttcatc gagctgacct tcgagggctt
     2161 cgccgaggac gagcgaggcc aggaccccgt gacccgcccc gactacgtcg cctcggccga
     2221 cgacaacgtc atcatcgagg gcatcgcggg ctcgccctcc tcgttcggct gggtgggctt
     2281 cgccttctac cggaacaacc aggacgcggt gaaggcgctc gaggtcgacg gggggagcgg
     2341 ctgcgtcgcg ccctcggagg agacgatcgc cgacggctcc tatccggtct cccgctcgct
     2401 gtacatctac gtgaacgacg cgagggcggc cgagagcgcg gcaccccggg cgttcgtcga
     2461 cttctacgtc tccgatgagg ggctcgcctc cgtggcggag gtcggctacg tggagctgcc
     2521 tccggagcgg atcgaggcca cccgggcggc ctgggaggag ggccggccct agaagatggc
     2581 ctggaagagc ttggccatgg cgaagctgag cagcatgcag gcgggccagg tcaggatcca
     2641 ggtccccacg acctcgccgg ccaggcccca ccgcaccgcg gacagcctcc gcgtggcccc
     2701 cacgcccatg atcgaggccc cgatcgcatg ggtggtggag agggggatcc cgagacgcga
     2761 cccgcccaag atgatgaggg ccgcggaggt ctccgcggcg aagccgttca ccggctccag
     2821 cttcgtcatc cgcagcccga tcgtgcggat cacccgccac ccgccgacgg ccgtccccac
     2881 cccgatcgtc gtggccacga cgaagaggac ccagagcggg atctcgagct cgggcaggtc
     2941 gccgttgagg aagagcgcca gggcgatgat ccccgcgccc ttctgtccat cgttcgaccc
     3001 gtggctgaac gccatgaagg cggcggagag gatctgcccc ttaccgaagg ttcggcggac
     3061 ggtcctgggc cgggcccggc ggaacgccca gtagatgccg accatgagga gcaggccccc
     3121 gaagaacgcg atcacggggc tggtgaagag gctgtagacg accttctggg tcccggacca
     3181 ctccagcgcg ttcaggcctc ccgccgcgaa gcccgcgccg aggagtgagg ccacgagggc
     3241 gtgggtctcg ctggaaggca tcccgaagac ctgcgccccc atcgcccaca gggccgcggc
     3301 gaagacggcc gcgccgatcg tctcgagcgt cacgacatcg gggctgacga tgccctgggc
     3361 gatcgtctcc gccacgagcg tgccggtgac gagcgcgccc accaggttga ggatggcggc
     3421 catgacgacc gccgcgtacg gacggagcac gcgcgtggac acgaccgtgg cgatcgcgtt
     3481 cggggcgtcc gtccacccgt tggcgaactg gaagagcagc gtcaggaaga tgaccagcgc
     3541 gagcggcatc taggcctgct tcagcgcgat cccctcgagg gtgttcgcca cgtcctcgag
     3601 cttgtccatg gccttctcga gctggtcgat gatgtccttc cagcgcagga cctccatcgc
     3661 ctcgaactcc ccggagaaca ggtccgcgac gccctgccgg tacactcggt ccgcctcgtt
     3721 ctccagccgg ttgatctcga tccagtaccg ggagaggcgg tcgaaggcgg ggagcgtctg
     3781 catcgcctcc agggtggcct tggccgcctc ggagagcagg tcgatctgcg ccttcatctc
     3841 ctggatcggc cgatcgatgt tgtggaggac caggaagtcg atcgccgcgt ccatgtggtc
     3901 gaggacgtcg tccatgttgc tggccagctc gtggacgtcc tcgcggtcga tcggggtgac
     3961 gaaggtcgtg ttgaggcggc gcatcacctc gtgcgtgacc tcgtccccct cgtgctcgag
     4021 gtcgtgcacc cggcgggccc tccgcttcac gtccgtgaac tcggtgacga tgccgcggaa
     4081 cacctcggtg gcctggacca agatctcggc ctgcttgatg aagaggtcgt agaaggactc
     4141 ctcgcgcggg atcagacgga acttcacgca gcctccctcc gcatgcgaat cggcgccgcg
     4201 cgcatggtac ccggatgcag ggtcgactcc caacatgccg gcccggccat gttcacccac
     4261 cgttcacccc gcgtccatgt gcgagcctcg cggcctccct accgtgaggc ccgtgaggtt
     4321 gccgggaggg tcgggggccg ccgagcggat cgagggcttg aggggggacc cgcgtcgacg
     4381 ccgccgcgag cggtcggtcg gctcggtgtt cttcgccgcg gccgccctgt cggtcgtggt
     4441 gagcgcggcg atcgtcctct cgctggcggg cgaggccgcc tcgttcctgt cgaaggtcga
     4501 tccgagctcg ctgtggtctg aggggtggtt cccgcgcagg agcctgtacg acctccggac
     4561 gatcgtcggc gggacgatca tggtcgcggg ggtggcgatg gtcgtcgcgg cgcccctggg
     4621 gctcttcacg gccatgtacc tgtccgagct cgcgagccct cgggcccgcc gctggctgaa
     4681 gccggtcgtg gaggtcctgg cggggatccc cagcgtggtg ctcggctatg tcgccctcgc
     4741 cttcatcaac cccgagctcg tccagcggtt cctcggcggg acctccccct tcagcatgct
     4801 gggagcgggg gtcgcggtgg ggatcctggt gaccccgctc gtggcgtcgg tggccgagga
     4861 cgcgctccac gcggtcccgc aggcgctgcg ggaggcctcc tccgggctcg gggcgaagcg
     4921 gacgacgacc acggttcggg tcgtgttccc cgcggcggtc tccgggatcg tggcggcgct
     4981 gatcctgggc ctctcccgcg ccatcgggga gaccatgatc gtggccatcg cctccggctc
     5041 caccggaggc tcgctcctct ccttcgacat cgtggggccg gggcagacga tgacgggggc
     5101 gatgaccgcc ctggccgtcg ggtccgaccg cgtggccgtg ggcgggggag gggccacggt
     5161 caaccccgtg gacgcgctct tcttcgtggg cctcctgctc ttcgtcatga ccttcgcgct
     5221 gaacgtggtc agcgagcggc tcgtgcggag gatgagggtc tgatgggagc cgcccggacg
     5281 cttggggcca cgccggcccg ggtcgcgacc caggacgcgc tccgggggac gcggatggat
     5341 ctcggcggcc tgctcctcca cgggctgctg ctcggttcgc tcctcctgac gctcggggtg
     5401 ctggccacgc tgctcctgca ggtggggacg accgcgtggc cggtgttcgc ggaccgaggc
     5461 ctcgacgtcg tgacgtcgaa cctctcctcg atcgcctcgc gggccggggt ctggcagggg
     5521 atccagggct cgatgtggct gatggccttc gtcgtcctct tctccttccc gctggggatc
     5581 ggggccgcga tctacatcga ggagtacgcg aggggtacgc ggctcacgcg gttcatcaac
     5641 gcgaacatcc gcaacctggc gggggtgccc tcggtcgtct acgggctgct cgggctggcc
     5701 atcttcgtga agctgctggg aggggtcacc ggaggccgga gcctcatctc cggggggatg
     5761 accctcgccg tcctcgtcct cccgatcgtc atcatcaccg cgtcggaggc gctgcgtgcg
     5821 gtgccgggcg ggatccggga ggcggcctac ggcgtgggcg ccacccgctg ggagacgatc
     5881 cggagccacg tcctccccta cgccgccccc ggcatactca ccggcacgat cctggcgctg
     5941 gcccgggcct tcggggagac cgcgcccctc ctaatggtcg gggtggccac ggggttcctc
     6001 tcgctgggtg ggggcgggct cgtcgatcag ctccaggggc cctacacggc cctcccggcg
     6061 atcatcttcc gctggtcgaa gctcccccag gccgacttca gggaggggct ggccgcggcg
     6121 gcgatcgtgg tgctcctggt cgtgctgctg gccgtcaacg cgctggcgat catcctgagg
     6181 aaccgatatg agcggaggtg gtgaggtccc ggtggagacg cggctggagc atcctgagtc
     6241 ccgagtggag gtccggtccg cgggggaggc gatcctggac ctgcggaacg tctcggtctc
     6301 ctacggggac gtgcgcgcgg tcgaggacat cacgctctcg atccgggagc acgagatcac
     6361 ggcactgatc gggccctcgg ggtgcgggaa gaccaccgtg ctccggagcc tgaaccggat
     6421 gaacgacctg atcgagggcg cccgcgtgga cgggaggatc ctctaccacg gggtcgactt
     6481 gtacgacgag cgcgtcgacc ccgtcgaggt ccggaggcgc atcggcatgg tgttccagaa
     6541 gccgaatccc ttcccgaagt cggtcttcga caacgtggcg ttcggacccc ggatctcggg
     6601 cttcgaggga gacctcgagg agcgggttcg gtgggctctg acgaaggcgg cgctctggga
     6661 cgaggtgaag gacacgctgc gggggcccgc ggtggcgctc tccggcgggc agcagcagcg
     6721 cctgtgcatc gcccgggcga tcgccgtgga gcccgacgtg atcctgatgg acgagccgtg
     6781 ctcgtcactc gaccccatcg ccaccctgaa gatcgaggac ctgatgcgcg agctggtgag
     6841 cgactacacg atcgtgatcg tcacccacaa catgcagcag gccgcccgcg tgagcgaccg
     6901 aaccgccttc ctcacgatcg acctcgaggg cggcagccgc gcggggcggc tggtcgagtt
     6961 cgacgacacc gaggtcatct tcaccaaccc ctccgatccg aggaccgagg actacgtcac
     7021 gggacggttc ggatgagcgc ggagccgagg cgccgcttcc acgacgagct ggcgggtctc
     7081 gaggcggcga tcggtgagat gggcgagcgc gcggagcggg cggtcggcgg ggcgatggcg
     7141 gcgctggcca cgggcggggt ggccgacgcg gtggtcgagg cggacgatgc gatcgacgac
     7201 cgatacctgg acgtcgagcg gcggatcctc gacctcctgg cgcggcagac gcccgtggcc
     7261 tccgacctcc gcctcgtctc cgcgatcctc cacatcaacc tccacctgga gagggtgggg
     7321 gacatcgcgg tcaacatcgc caagatcgcg aacgccacgc gcgacctgcc gcgcagcgag
     7381 ccgatcctcg ccgccctggt cgagatgggg gacgtggtcc ggcccatgct ccgagcgtcg
     7441 atggaggcct tcgcccggcg cgacctggac ctggcccgcc ga
//
LOCUS       DASUTW010000105         4626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_937130, whole genome shotgun sequence.
ACCESSION   DASUTW010000105 DASUTW010000000
VERSION     DASUTW010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937130"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VF058_03365"
     CDS             complement(<1..335)
                     /locus_tag="VF058_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6207376.1"
                     /translation="MRFAIHAPNFGAPGDLLDLALAAEVSGWHGFFLWDHVGSPAEAP
                     MEVLDPWVMLGAIAARTERIRLGTAITPLARRRPHVVAREAATLDVLSGGRAVLGVGL
                     GNPPVAEFAA"
     gene            complement(340..849)
                     /locus_tag="VF058_03370"
     CDS             complement(340..849)
                     /locus_tag="VF058_03370"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX6207377.1"
                     /translation="MARITLAQGDITQEEVDAIVNAANSKLMGGGGVDGAIHRKGGPA
                     ILEECGQIRAERYPEGLPTGEAVATTAGDLPARWVIHTVGPVYAKGEDRSGLLASCHT
                     ESLRVADELGARTVAFPAISTGVYGYPVEEAAPVAIAAVREAHTQVDEVRFVLFDEAT
                     YRAFERALG"
     gene            873..2021
                     /locus_tag="VF058_03375"
     CDS             873..2021
                     /locus_tag="VF058_03375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6207378.1"
                     /translation="MPDVIVVGGGVIGAACAREAARRGMSVTLVERSELAAGASGRNH
                     GLLLTPPDPALVPMFHASREIYAEAASGAVVPFDLDPEPLGFLIVATEDEAELAAGRE
                     EAEASAASGVARAELDADGVRSLEPGITDEVVEGWLLEDGWRLNPSALTVSLALSPGV
                     DVRRHLNVRALLAGDGRARGVVTDEGRIEADEVVVAAGPWTPGLLRPLGIELPVGGAR
                     GWLVHLGCEDPPVVRLVERAGWHTVGGEEAMSRLIAEGLIEALPRPDVGTIVQPNPDG
                     TVLVGGSRQRVVTPEPEDPSVPRELTRRAVDLVPPLGDARILGSWWGIRPVTPDGPPI
                     VARVADGLLVATGHGSQGVILGGGTGRLVGSILAGEEPPFDPAPFALR"
     gene            complement(2023..2364)
                     /locus_tag="VF058_03380"
     CDS             complement(2023..2364)
                     /locus_tag="VF058_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX6207379.1"
                     /translation="MDRRRFHELVDEAMGGLPAWVRERIENVAVIVEDEPPEDDPDLL
                     GLYQGVPLTERGADYFGVLPDRIRLFRRVIEEEAQSEEDVTRVIRETVIHEVAHFFGI
                     SDERLGELDRD"
     gene            complement(2364..3842)
                     /locus_tag="VF058_03385"
     CDS             complement(2364..3842)
                     /locus_tag="VF058_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX6207380.1"
                     /translation="MSDPWTDRGPTPVYRAEPFAAQAEPDRLPSPSAGPERRRAFRRA
                     LVIVLVITAAAGIARFWSLGRPAEKVFDEVYYASDACLYAGHPFEDCGLESAAERSWV
                     HPPLGKWLIALGIDAFGNDPFGWRFTGALVGTASVALAGAFAFLLWGRPLWAGVAALL
                     LATEHLHLVQSRVAMLDVFLAFFVLLGFTLLLWDRVRQKRLDALAAANEAAPYLAPSG
                     PRWIRVAAGASFAAATASKWSGIFAWLGGLVLAVMWERGRRARAGVERPLLAALRQEW
                     ASLALAFVAAPLLVYFLSWVRWLGDHGWSLPRLWSNHLSMADYHLDLEPFSESGEPIH
                     PYMSEAWTWLLLLRPVAYYYESMGDRAAEILGIGNPLLFWGALAFLPYLAYVWIRRRD
                     WRAGAILVPVLVLYAPWLFVTRPLFLFYMVPVTPFLALGATYALRDVSRVRGAGRPSL
                     RLAAGVAVAVAVGLFVFFWPVLIGDPISKGAWDARIWFSGWI"
     gene            3911..>4626
                     /gene="rsmI"
                     /locus_tag="VF058_03390"
     CDS             3911..>4626
                     /gene="rsmI"
                     /locus_tag="VF058_03390"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020512908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX6207381.1"
                     /translation="MAGTLILVGTPIGNLKDASERARETLARVDLIACEDTRRTGRLL
                     QSLRIQRRARLVSYFEGNERRQTPYLIEQLQRGSDVALVTDGGMPAVSDPGFRLVRAA
                     IAAGIEVDVVPGPAAAIAALVVSGLPTDRFAFEGFLPRKARVARLQELAGDPRTLLFF
                     ESPQRLRDMLRDAARALGPDRPAAVVRELTKLHQEIRRGTLGELADATPDEVKGEVTV
                     VVGGAEPAGPDLTALASQVN"
BASE COUNT          668 a         1645 c         1741 g          572 t
ORIGIN      
        1 gcggcgaact cggcgacggg cgggttcccc agcccgacgc ccagcaccgc gcgccccccg
       61 ctcagcacgt cgagcgtcgc cgcctcccgc gccacgacgt ggggtcgtct cctggcgaga
      121 ggcgtgatgg cggtccccag gcggatccgc tcggtccggg ccgcgatcgc cccgagcatc
      181 acccaggggt cgaggacctc catcggcgcc tccgctggcg accccacgtg gtcccagagg
      241 aagaacccgt gccagccgct cacctcggcg gcgagggcga ggtcgaggag gtcacccggg
      301 gccccgaagt tcggggcgtg gatcgcgaag cgcacgggct cagcccagcg cccgctcgaa
      361 ggcccggtag gtggcctcgt cgaacagcac gaagcggacc tcgtcgacct gggtgtgcgc
      421 ctcccggacg gcggcgatgg ccacgggggc ggcctcttcc accggatacc cgtagacgcc
      481 cgtggagatc gcggggaagg ccacggtccg tgccccgagc tcgtcggcga cgcggagcga
      541 ctcggtgtgg cacgaggcga ggagtcccga tcggtcctcg cccttcgcat agaccggccc
      601 gaccgtgtgg atcacccagc gagccggcag gtcgcccgcc gtcgtggcca cggcttcgcc
      661 ggtggggagg ccttccgggt agcgctcggc gcgaatctgc ccgcactcct cgaggatcgc
      721 cgggccgccc ttccgatgga tcgcgccgtc cacgcctccg ccgcccatca gcttggagtt
      781 cgccgcgttg acgatcgcgt cgacctcctc ctgggtgatg tccccctgcg ccagcgtgat
      841 gcgcgccatg catccagtct aggctcggtg cgatgccgga tgtgatcgtg gtgggaggag
      901 gcgtgatcgg cgcggcgtgc gcgcgcgagg ccgctcgccg cggcatgtcg gtgacgctcg
      961 tggagcggtc cgagctcgcg gccggcgcct cggggaggaa ccacgggctc ctcctgacgc
     1021 cgcccgaccc ggccctggtc ccgatgttcc atgcctctcg agagatctac gcggaggccg
     1081 cctcgggcgc agtcgtgccg ttcgacctcg atcccgagcc gctcggcttt ctcatcgtgg
     1141 cgaccgagga cgaggcagag ctcgcggccg ggcgagagga ggctgaggcg agcgcagcct
     1201 cgggtgtggc ccgcgccgag ctcgatgcgg acggcgtccg gtccctggag ccggggatca
     1261 ccgacgaggt ggtggagggc tggttgctcg aggacgggtg gcgcctcaac ccctcggcgc
     1321 tcacggtgtc gctcgcgctg tcgcccggcg tggacgtgcg tcggcatctg aacgtccgag
     1381 ccctcctggc cggggacggg agggcccgag gagtcgtgac cgacgagggg aggatcgagg
     1441 ccgatgaggt cgtcgtggcg gcgggcccct ggaccccggg cctgctccgc cctctgggga
     1501 tcgagcttcc ggtgggcggg gccagagggt ggctggtcca cctcgggtgt gaggaccctc
     1561 ccgtggtgag gctggtcgag cgcgcgggct ggcacacggt cgggggcgag gaggcgatgt
     1621 cgagactcat cgccgagggg ttgatcgagg ccttgccgcg gcccgacgtc ggcacgatcg
     1681 tccagccgaa ccccgacggc acggtcctcg tgggcggctc ccgccagcgg gtcgtgaccc
     1741 cggagcccga ggacccctcg gtcccccgcg agctcacgcg ccgcgcggtg gacctcgtgc
     1801 ccccgctcgg cgacgcgcgg atcctcgggt cctggtgggg gatccgtccg gtcacgccgg
     1861 acgggccgcc gatcgtggcg cgggtcgcgg acggcctcct ggtggcgacc ggccacgggt
     1921 cccagggggt gatcctaggg ggtgggaccg ggcggctcgt gggctcgatc ctcgcgggag
     1981 aggagcctcc cttcgacccg gcgcccttcg cgctccgctg actcagtccc ggtcgagctc
     2041 accgagccgc tcgtcggaga tcccgaagaa gtgcgcgacc tcgtggatca ccgtctcccg
     2101 gatcacgcgc gtcacgtcct cctcggactg ggcctcctcc tcgatcaccc ggcggaacag
     2161 ccggatccgg tccgggagga ccccgaagta gtcggcgccg cgctccgtga gcggcacccc
     2221 ctggtagagg ccgaggaggt cggggtcgtc ctcgggaggc tcgtcctcca cgatcacggc
     2281 cacgttctcg atccgctcgc ggacccaggc cggcagcccg cccatggcct cgtccacgag
     2341 ctcgtggaag cgtctccggt ccatcagatc caccccgaga accagatgcg ggcgtcccag
     2401 gcccccttcg agatcgggtc gccgatcagg acgggccaga agaagacgaa gagccccacc
     2461 gccacggcga ccgcgacgcc cgcagcgagc cgcagcgagg ggcgaccggc tccgcggacg
     2521 cgtgagacgt cccgcagagc gtacgtggcg cccagcgcca ggaacggcgt gaccggaacc
     2581 atgtagaaga ggaagagtgg gcgggtgacg aagagccacg gcgcgtagag gacgagcacc
     2641 gggacgagga tggccccggc ccgccagtcg cgacggcgga tccacacgta cgccaggtag
     2701 gggaggaacg ccagcgcccc ccagaagagg agcgggttgc cgatccccaa gatctcggcg
     2761 gctcgatcgc ccatgctctc gtagtagtag gcgacgggac ggagcaggag gagccaggtc
     2821 caggcctcgg acatgtaggg gtggatgggc tctccgctct cgctgaacgg ctccaggtcg
     2881 aggtggtagt cggccatgga gaggtggttg gaccagaggc gcggaaggct ccagccgtgg
     2941 tccccgagcc agcggaccca gctcaggaag tagacgagca gcggcgccgc gacgaaggca
     3001 agggcgagcg aggcccactc ctggcggagg gcggcgagga gagggcgctc gacgcccgct
     3061 cgagccctgc ggccccgctc ccacatgacc gccaggacca gcccgcccag ccaggcgaag
     3121 atccccgacc acttcgaggc ggtggccgcg gcgaacgagg cgccggctgc cacccggatc
     3181 cagcgaggac ccgacggggc gagatagggc gccgcctcgt tcgccgcggc caacgcatcc
     3241 aggcgcttct gccgcacccg gtcccagagg agcagcgtga accccagcag gacgaagaac
     3301 gcgaggaaga cgtcgagcat cgccaccctc gactgcacga ggtggaggtg ctcggtggcc
     3361 agcagcagcg ccgctacgcc ggcccagagc gggcgccccc acagcaggaa ggcgaaggcg
     3421 ccggccagcg ccaccgaggc cgtgccgacc agggccccgg tgaaccgcca cccgaacggg
     3481 tcgttgccga acgcgtcgat ccccagggcg atcagccact tccccagcgg ggggtggacc
     3541 caggaccgct ccgcggcgct ctcgagcccg cagtcctcga acgggtgccc cgcgtagagg
     3601 cacgcgtcgg aggcgtagta gacctcgtcg aagaccttct cggcgggccg gcccagggac
     3661 cagaaccggg cgatccccgc ggcggcggtg atgacgagga cgatcaccag ggctcgacgg
     3721 aaggcccggc ggcgctccgg gccggccgag ggagacggga gccggtcggg ctcggcctgc
     3781 gccgcgaagg gctcggcgcg gtagacgggg gtggggcccc ggtccgtcca ggggtcgctc
     3841 acgatgggag catgccacgg ctccgagccc tcgtcgcgga tgctggcgcg ctcccgccta
     3901 tcatcgatgg atggccggca cgctgatcct cgtggggacg ccgatcggga acctcaagga
     3961 cgcgtccgag cgggcccggg agacgctggc ccgcgtggac ctgatcgcct gcgaggacac
     4021 gcggaggacc ggacggctcc tccagtcgct gaggatccag cgccgggccc gcctcgtctc
     4081 ctacttcgag gggaacgagc gcaggcagac cccctacttg atcgagcagc tccagcgggg
     4141 gagcgacgtg gcgctggtca ccgacggggg gatgcccgcg gtctcggacc ccgggttccg
     4201 gctggtgagg gcggcgatcg cggccggcat cgaggtggac gtggtgcccg ggccggcggc
     4261 cgcgatcgcc gccctcgtgg tcagcggcct ccccacggac cggttcgcct tcgagggctt
     4321 cctccccagg aaggcgcggg tcgcccgcct acaggagctg gcgggcgacc cgcgcaccct
     4381 cctcttcttc gagtcgcccc agcgtctccg ggacatgctc cgggacgccg cccgggccct
     4441 gggacccgac cgaccggccg ccgtcgtccg cgagctcacg aagctccacc aggagatccg
     4501 gcgggggacc ctcggggagc tggccgacgc cactccggac gaggtcaagg gcgaggtgac
     4561 ggtcgtcgtg gggggcgcgg agcctgcggg cccggacctg acggcgctcg catcgcaggt
     4621 caacga
//
LOCUS       DASUTW010000106         2152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1066700, whole genome shotgun sequence.
ACCESSION   DASUTW010000106 DASUTW010000000
VERSION     DASUTW010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066700"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VF058_03395"
     CDS             <1..540
                     /locus_tag="VF058_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207382.1"
                     /translation="PPPAPAGRRYFAQIRTDLPEPHTETVDVVVDEAASIVRVHIATG
                     TTDLLLAPEGDILAGTSNGEPIELPWGPDRHLDYLSPVFNAISAARLDGTAEIDVLYL
                     DPVTCEPRDMVQRYEWLGTERIATPVGAFEAGAWRYTAVASGFSRRLWVAGDLVVAYE
                     TVFELIEYEPGQTGPFPSA"
     gene            574..2010
                     /locus_tag="VF058_03400"
     CDS             574..2010
                     /locus_tag="VF058_03400"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6207383.1"
                     /translation="MAERRFLERLARLAVPALIGMVGAWLALLTAGRHDYDLGPFRVE
                     FFGRPGPSATELSLPPFGRLQADTHLAPLKLSATLQEVAAVELGDIVQHKGIEGLTLE
                     VEEEAPGVLLSYAIRSLAVVLAGAALAALLVFRERWRPAVVAIVSSGLVFGGMAGISW
                     LTFRPEAFLSPTFSGSLALAPRLVGPIREAGGRIEDFRLELERLVGGAVSAYTTIAAA
                     PPTTGADIVALHISDVHLSPLGMDFAQRLASAFEVDLVLDTGDLTSFGSSLEQGIASR
                     IREFGVPYVFVRGNHDSALTVNEVLAQPNAVVLGHEVQDVAGLRIYGAPHPLFTPTLE
                     RDYPGEEIEDTLLAVGEVVSEEVAATRPDLLVVHDDRMAEASTGLVPVVASGHFHETT
                     ARLVDGTILLRVGTTGAGGLDTFTAPQPIPLSAQLLYFDGDPVELLAWDTVLLDPITR
                     DLTVRRHLPTEIGEEEEPAPQGEPAPTP"
BASE COUNT          273 a          813 c          750 g          316 t
ORIGIN      
        1 cccccccccg cccccgccgg ccgccgctac ttcgctcaga tccggaccga cctccccgag
       61 ccgcacaccg agacggtgga cgtcgtcgtg gacgaggcag cctcgatcgt ccgggttcac
      121 atcgccacgg gaacgacgga cctcctgctg gcgccggaag gcgacatcct cgccgggacg
      181 agcaacggcg agccgatcga gctcccgtgg ggccccgacc gccacctcga ctacctctcc
      241 cccgtcttca acgcgatcag cgcggcgcgg ctcgacggca ccgccgagat cgacgtgctc
      301 tacctcgacc ccgtcacctg cgagccccga gacatggtcc agcgctacga gtggctgggg
      361 accgagcgca tcgccacgcc ggtgggggcg ttcgaggccg gcgcctggcg ctacacggcc
      421 gtggccagcg gcttctcgcg ccgcctctgg gtcgccggcg acctcgtggt cgcctacgag
      481 acggttttcg agctgatcga gtacgagccc ggccagaccg ggcccttccc ctccgcgtag
      541 ggggcggccc gccctccggg tacagtgggg cccgtggccg agcgtcgctt cctggagcgg
      601 ctcgcgcggc tcgcggtgcc cgccctcatc ggcatggtgg gagcgtggct cgcgctgctc
      661 acggcgggcc ggcacgacta cgacctgggg ccgttccggg tcgagttctt cggtcgcccg
      721 ggtcccagcg ccaccgagct ctcccttccg ccgttcggca ggctgcaggc cgacacgcat
      781 ctcgcgccgc tgaagctctc ggccaccctc caggaggtcg ccgccgtcga gctcggcgac
      841 atcgtccagc acaaggggat cgaggggctc acgctggagg tcgaggaaga ggccccgggg
      901 gtgctcctct cgtacgccat caggtcgctc gccgtcgtcc tggccggggc ggcgctcgcc
      961 gcactgctcg tcttccggga gcggtggcgg ccggcggtcg tggcgatcgt ctcctcgggc
     1021 ctcgtcttcg gaggcatggc cggcatctcc tggctcacgt tccggccgga ggccttcctc
     1081 agcccgacct tctcgggctc cctcgccctg gccccgcgcc tggtggggcc tatccgggag
     1141 gcgggggggc ggatcgagga cttccgcctc gagctcgagc gcctggtcgg cggcgccgtc
     1201 tccgcgtaca cgacgatcgc ggccgcgccc cccacgaccg gggccgacat cgtggcgctc
     1261 cacatctccg acgtgcacct ctccccgctc ggaatggact tcgcgcagcg cctcgcctcc
     1321 gcgttcgagg tggacctcgt cctggacacg ggagacctca cctcgttcgg gagctcgctg
     1381 gagcagggga tcgcctcgcg catccgggag ttcggcgtcc cgtacgtgtt cgtgcggggg
     1441 aaccacgaca gcgcgctcac ggtgaacgag gtgctggccc agcccaacgc ggtcgtcctc
     1501 gggcacgagg tccaagacgt ggcgggtctg cggatctacg gcgccccgca ccccctcttc
     1561 acgcccacgc tcgagcgcga ctacccgggc gaggagatcg aggacaccct gctggcggtg
     1621 ggagaggtag tgtccgagga ggtcgcggcg acgaggccgg atctgctcgt ggtgcacgac
     1681 gaccgcatgg ccgaggcttc gaccgggctg gtccccgtgg tggccagcgg ccacttccac
     1741 gagacgacgg cgcggctggt cgacgggacg atcctgctgc gggtggggac gacgggggcc
     1801 ggggggctcg acaccttcac ggcgccccag ccgatcccgc tctcggcgca gctgctctac
     1861 ttcgacggcg accccgtgga gctgctggcc tgggacacgg tcctgctcga cccgatcacc
     1921 cgtgacctca cggtgcggcg ccaccttccc acggagatcg gggaagagga ggagccggcc
     1981 ccccaggggg agccggctcc cacgccttga tgatcagctc ccgtcggccg gagggagctc
     2041 ctgccactcg ctctcgtcga cgctctggga ccggcggcgc ctgaagaccg ccgcgacggc
     2101 gccgatcccg ccgaggatcg cggcccagag gaagaacctc ttcttcccgc tc
//
LOCUS       DASUTW010000107         4261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1067184, whole genome shotgun sequence.
ACCESSION   DASUTW010000107 DASUTW010000000
VERSION     DASUTW010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067184"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VF058_03405"
     CDS             complement(<1..531)
                     /locus_tag="VF058_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6207384.1"
                     /translation="MKILPPARLGPLVARPRLERRLEDALSSRLTVVLAGAGFGKTTL
                     LCAWAAAARSAWYTLDATDRSLPVLVRGLADALRLRVPGLHAEVAMALEGAAGPNADD
                     PSRAQALASLVAEGLARELDEDLLLVLDDVHEADGSPASVRLIDELSRQAPQPLHVVL
                     ASRTEVPFQIERLRGRG"
     gene            complement(645..2267)
                     /gene="groL"
                     /locus_tag="VF058_03410"
     CDS             complement(645..2267)
                     /gene="groL"
                     /locus_tag="VF058_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797913.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX6207385.1"
                     /translation="MPGKELKFHEDARRALEAGVDKLADTVSITLGPKGRNVVLDKKW
                     GAPTVTNDGVTIAKEIELEDPWENMGAQLAKEVATKTNDVAGDGTTTATVLARAMVKQ
                     GMKNVAAGANPMALKRGIEQAVTAAVEAIEKQSREVEGKDEIAHVAAISAADPKIGEI
                     IAEALEKVGKDGVVTVEESQTFGMELDFTEGMQFDKGYISPYFVTDQDRMEAILDEAY
                     LLLVGKKISSVQELLPVLEKVSQTGKPLVIIAEDVEGEALATLVVNKIRGTFKSAAVK
                     APGFGDRRKAMLEDMAILTGGQVISEEVGLKLESVSLDIMGTARRVVITKDDTTIVEG
                     AGEEPAIKGRIEQIRRQIDETDSDWDREKLQERLAKLSGGVALIKVGAATEVELKEKK
                     HRIEDALSATRAAVEEGIVPGGGVALIRAEEALDGLKLEGDEATGARIVRAALSEPSR
                     LIAGNAGFEGSVIVERLRGEKGSSGFDAAKGEWVDMIKAGILDPAKVTRSALQNAASI
                     AALVLTTESAVVEMPEEEDEAAAAAAGAGHMH"
     gene            complement(2278..2577)
                     /gene="groES"
                     /locus_tag="VF058_03415"
     CDS             complement(2278..2577)
                     /gene="groES"
                     /locus_tag="VF058_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002857590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX6207386.1"
                     /translation="MAKFGLKPLEDRVVVKPGEEEETTASGIVIPDTAKEKPQEGEVV
                     AVGPGRFSDQGERIALDVKEGDVVVYSKYGGTEIKVKGEEYLILSARDILAIVKK"
     gene            2874..3341
                     /locus_tag="VF058_03420"
     CDS             2874..3341
                     /locus_tag="VF058_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207387.1"
                     /translation="MDPSRFRAVAGLVAAAVVLGVFTLSPVSAHFTQNTKHLGKHAWQ
                     QFIKKRVTKSFVQEGKILSATVTGAGTENAEIVRGRGAVQAEGDVATDVEFTRKVHNC
                     TWTATYGEANLGVMAMVHPTGGNPKGLRVWLADDEGGPIVGNQRTFHLIVVCP"
     gene            3786..4241
                     /locus_tag="VF058_03425"
     CDS             3786..4241
                     /locus_tag="VF058_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207388.1"
                     /translation="MASPRFRAIAGLVAAAVVLGVFTLSPVSAHFTQNTKHLGNHAWK
                     QVIKKKADKRYLRKTKVVVVEDSITATSNKQTDALCPGGWQALGGGINFQSTDPAAEV
                     TWNEPLIDGDNLNAAGEGTHPAADGWRVRVDNDSGVTYTYAVGVICARA"
BASE COUNT          629 a         1541 c         1405 g          686 t
ORIGIN      
        1 cccgcggccg cgcagacgct cgatctggaa cggcacctcg gtcctcgatg ccagcaccac
       61 gtggagcggc tggggggcct gccgcgacag ctcgtcgata agcctgaccg aggccggcga
      121 cccatcggcc tcgtggacgt cgtccagcac gaggagaagg tcctcgtcca gctcccgagc
      181 cagcccctcc gccacgaggg acgccagcgc ctgcgcccgc gacgggtcgt ccgcgttcgg
      241 accggccgcc ccctccagcg ccatcgccac ctccgcgtgc agcccgggga ccctcagcct
      301 gagcgcgtcg gccaggcccc tgaccagcac cggcagggac cgatccgtgg cgtcgagcgt
      361 gtaccaggcc gatctcgccg cagcggccca ggcgcagagc agggtggtct tgccgaagcc
      421 ggcccccgcc aggaccacgg tcaggcggga cgagagcgca tcctccagtc ggcgctccag
      481 ccgcgggcgg gccacgagag gaccgagccg ggcgggcggg aggatcttca cggaaggcgt
      541 tgatctcacg ggcctcgtcc ctgctcgttc cggtgggccc atcctacggg aacgcgagag
      601 ggaccgggcg tgcgcccggt ccctctcgcg aacgaaaccg ccggtcagtg catgtggccg
      661 gcgcccgcgg cggcggccgc ggcctcgtcc tcctcctcgg gcatctccac cacggcgctc
      721 tcggtggtga gcaccagcgc cgcgatcgag gccgcgttct ggagcgcgga gcgcgtgacc
      781 ttggccggat ccaggatccc ggccttgatc atgtcgaccc actccccctt ggccgcgtcg
      841 aacccgctgg agcccttctc gccccgcagc cgctcgacga tcaccgagcc ctcgaagccg
      901 gcgttcccgg cgatcagacg cgagggctcc gagagcgccg cgcgcacgat ccgcgcgccc
      961 gtcgcctcgt cgccctcgag cttcagcccg tcgagcgctt cctccgctcg gatgagcgcc
     1021 acgccgcctc cggggacgat cccctcctcg acggccgccc gggtcgcgga gagcgcatcc
     1081 tcgatgcggt gcttcttctc cttcagctcg acctcggtcg cggccccgac cttgatcagc
     1141 gccacgcctc cggagagctt ggccagccgc tcctggagct tctcgcggtc ccagtcggag
     1201 tcggtctcgt cgatctggcg gcggatctgc tcgatccggc ccttgatcgc gggctcctct
     1261 ccggcgccct cgacgatcgt ggtgtcgtcc ttggtgatga cgaccctgcg ggcggtcccc
     1321 atgatgtcga ggctcacgct ctcgagcttc aggccgacct cctcggagat cacctggccc
     1381 ccggtcagga tcgccatgtc ctcgagcatc gccttccggc ggtcgccgaa gccgggcgcc
     1441 ttcaccgctg cggacttgaa ggtgccgcgg atcttgttga cgaccagggt ggcgagggcc
     1501 tcgccctcca cgtcctcggc gatgatcacc aggggtttcc cggtctggga gaccttctcc
     1561 agcaccggga gcagctcctg cacgctcgag atcttcttgc cgacgagcag gagatacgcc
     1621 tcgtcgagga tcgcctccat gcggtcctga tcggtgacga agtacggcga gatgtagccc
     1681 ttgtcgaact gcatcccctc ggtgaagtcg agctccatcc cgaaggtctg ggactcctcg
     1741 accgtcacca cgccgtcctt gcccaccttc tccagggcct cggcgatgat ctcaccgatc
     1801 ttgggatcgg ccgccgagat cgcggcgacg tgcgcgatct cgtccttgcc ctccacctcg
     1861 cgggactgct tctcgatcgc ctcgacggcg gccgtcacgg cctgctcgat accgcgcttc
     1921 agcgccatcg ggttggcgcc ggcggcgacg ttcttcatcc cctgcttcac catggcccgc
     1981 gccaggacgg tcgcggtggt ggtgccgtcc ccggcgacgt cgttcgtctt ggtggcgacc
     2041 tccttggcga gctgggcccc catgttctcc cacgggtcct ccagctcgat ctccttggct
     2101 atcgtcaccc cgtcgttcgt gacggtgggt gccccccact tcttgtcgag gacgacgttg
     2161 cgccccttcg gcccgagggt gatcgagacc gtgtcggcca gcttgtcgac cccggcctcg
     2221 agggccctcc gggcatcctc gtggaacttc agctctttcc ccggcatgaa ccctccctca
     2281 cttcttgacg atggccagga tgtcgcgggc cgagaggatc aggtactcct cgcccttcac
     2341 cttgatctcg gtacccccgt acttcgaata gacgacaacg tcgccctcct tgacgtcgag
     2401 cgcgatgcgc tcgccctggt ccgagaaccg gccggggccg acggcgacga cctcgccctc
     2461 ctggggcttc tccttcgcgg tgtcggggat cacgatcccc gaggccgtgg tctcctcttc
     2521 ctcccccggc ttcaccacga cgcggtcctc gaggggcttc agcccgaact tggccatacg
     2581 ttccctcctt gggtcgctgt tagcactcga tactgccgag tgccaagtct actgaccccc
     2641 tccgcaagcg gcaaccccag cccctttcat cccaccctaa ggtccgtcgc tacggtcctg
     2701 cccatggcgg aagggaggga cgcgggaagg agcggcgtcg ctggcaccgt tggcgcctcg
     2761 cagggccggc cgatcgcatc gagaggcgcc gccccgggct ccgatcgcgc agcccgaccc
     2821 agctctcatc cgcccgtgat cggcgaagca tccgacgaga gagggaggga gcaatggatc
     2881 catcgcgctt ccgcgccgtc gcgggtctgg tggcggcggc cgtcgtcctc ggggtcttca
     2941 cgctgagccc cgtgtcggcg cacttcacac agaacaccaa gcatctgggc aagcacgcct
     3001 ggcaacagtt catcaagaag agggtgacca agagcttcgt ccaggagggg aagattctct
     3061 ccgcgacggt cacgggtgcg ggaacggaga acgccgagat cgtgcgcggt cgcggcgcag
     3121 tccaggccga aggggacgtc gcgaccgatg tggagttcac tcgaaaggtg cacaactgca
     3181 cgtggacggc cacctatggg gaggcaaacc tcggcgtgat ggccatggtg caccccacgg
     3241 gaggcaaccc gaaggggctc agggtctggc tggcggacga tgaagggggt ccgatcgtcg
     3301 ggaaccagcg gaccttccac ctcatcgtcg tctgtccgta gcgccatcgg gccgagcggc
     3361 gcttggcggg cagcctgcgg ggctccccgt agatcgtccc ccccgaggcg gcgtagacga
     3421 tcttcctcgt ccccgatcgg gcggcggtca gcgtgcccag cacgttgatg tcggcgtcct
     3481 tgcgcggatc ctcgacgagg gccgcacgcc ggaccgcgcg gcgactccgc ccccttcatc
     3541 ccgccctaag gggccgctgc tacgatcctc ccacggcaga aggagggatg agacacggcg
     3601 ggaggagaga agggacggtg gaggagcggc ggcttcgctg gtaccctatg cgcctccgca
     3661 gggtcggtcg atcgcatcga gaggctaccg ccccgggctc cgatcgagga gtccacctca
     3721 cctctcttcc cgcccgtgat cgccgagttc cggagcgaag catccgacga gagagggagg
     3781 gagcaatggc ttcaccgcgc ttccgcgcca tcgcgggtct ggtggcggcg gccgtcgttc
     3841 tcggggtctt cacgctgagc cccgtgtcgg cgcacttcac gcagaacacc aagcatctgg
     3901 gcaaccacgc ctggaagcag gtgatcaaga agaaggccga caagaggtac ctgcgcaaga
     3961 cgaaggtcgt ggtggttgag gactcgatca cggctacgtc caacaaacag acggacgcgc
     4021 tctgtcctgg cggatggcag gcgctgggtg gcgggatcaa cttccagagc accgaccccg
     4081 cggccgaggt cacgtggaac gagcccctga tcgacgggga caacctcaac gccgcgggcg
     4141 agggcaccca ccccgccgcc gacgggtggc gcgtgcgcgt ggacaacgac agcggggtca
     4201 cctacaccta cgcggtcggc gtcatctgcg cgcgggcctg agcgagcgag gggggggggg
     4261 g
//
LOCUS       DASUTW010000108         3877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1067379, whole genome shotgun sequence.
ACCESSION   DASUTW010000108 DASUTW010000000
VERSION     DASUTW010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067379"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..586)
                     /locus_tag="VF058_03430"
     CDS             complement(35..586)
                     /locus_tag="VF058_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207389.1"
                     /translation="MTRLFRFGGYFASIVLILFGIGAAVLGTLGFLEVRDTIAQENIT
                     ATPDAAEIEGVDLEPGQEIRTGGEAKEFAEIIRTHALEATGGRAFAEIPRYLTETGDE
                     TDDEAAAATTEEGRPVENPVRGIWNTATAFTTALNTAYLAEQIALFTIAMGVALVLVG
                     IGFLVLTIAALRGPVFERHPDTT"
     gene            743..1042
                     /locus_tag="VF058_03435"
     CDS             743..1042
                     /locus_tag="VF058_03435"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX6207390.1"
                     /translation="MVVTVVAAHVAEERGSEFRHSFAGATADLPPGIVQTFLLRAPES
                     HEWRILTVWRSREELEAYRAAVEVPEAIRMFRAVGSEPVMGEFDVLEQAVETAGA"
     gene            complement(1039..2610)
                     /locus_tag="VF058_03440"
     CDS             complement(1039..2610)
                     /locus_tag="VF058_03440"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX6207391.1"
                     /translation="MGLKAHPRWERAVRALRRGLRGARGRGAVVLASLLLFPLGITLI
                     LRTVPTLDPIFESPRFHIVVVSAIALCALIVALLTAISAAKAREAAPVLLAMGCLAIG
                     LLMFAHGMTTPGIGGRGFSFWVIRFPVLALAGFAACLALAAAPERATKRLVGRFPRGS
                     LLGAAALLSAFSVVLIARPHALSGLRPIPGEEAARWVLIAGTTVTLLGVGAVHWHRWR
                     LGRDRVQLGLMSAAWLSANAAVALEVGHLWRVSWWDYHVYLLAGFAAAAWSVTTQSRQ
                     TRSVREALATVSIRDPIQLVSRGHPEALDALVGAVEAKDSHMRGHAARVAEVAMRIGL
                     RMDMGADELRGLAQGAFLHDIGKIGVPDDVLHKRDRLTREDWTRIEQHPVIGGEMAGR
                     APSLHDSLAVIRHHHERWDGSGYPDRLTGDEIPIAARIATVADVWDALTSDRSYRPAW
                     PADRALAYIAGAAGHLFDPVAVEAFLDLLAERGLWPDRLRPDVDALASMAEACHPRTN
                     GHAASVAVGKPERSA"
     gene            complement(2652..3554)
                     /locus_tag="VF058_03445"
     CDS             complement(2652..3554)
                     /locus_tag="VF058_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX6207392.1"
                     /translation="MSEDPHWPRASEWLAGSHAPHPRRRLAVLGVPLHLGSISPSRAD
                     LAPAAIREALGRYSTWDGETRGDIEVVAAEDHGDLPLADVAPDDALDSVVAAMQGLDA
                     HALVLLGGDNSVTRPGVHGLGVPLDRLGLLTLDAHFDLRETDGGMRNGNPVRALLEDG
                     LPGENVVQIGIQPFANSPMHAEVAQEAGITFVLAGEVRSRGIGAVLTEALEHLDARAD
                     SIYLDVDVDVLDRGFAPATPGSRPGGLAPWEIRRAARLAGRHPKVRAVDLVEIDPTVD
                     VADVTTLAAAATLLAFAAGLAARS"
     gene            complement(3551..>3877)
                     /locus_tag="VF058_03450"
     CDS             complement(3551..>3877)
                     /locus_tag="VF058_03450"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006236845.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX6207393.1"
                     /translation="SPNRETEGMADGSDAIADWPILNALVNTAAGAHWVSVHHGGGVG
                     IGSSIHAGMVVVADGSDEAALRLERVLTTDPGTGVVRHADAGYERAIEVARERGIRIP
                     MDEEGS"
BASE COUNT          544 a         1417 c         1404 g          512 t
ORIGIN      
        1 cccccccccc cccccccccc gccccaggga agggtcaggt cgtgtcgggg tgccgctcga
       61 acaccgggcc cctcagcgcc gcgatcgtga gcacgaggaa cccgatgccc accagcacca
      121 gagcgacgcc catcgcgatc gtgaacagag cgatctgctc cgcgaggtag gcggtgttca
      181 gcgccgtcgt gaacgcggtg gccgtgttcc agatgccgcg gacggggttc tcgaccggcc
      241 gtccctcctc cgtggtcgcg gccgcggcct cgtcatccgt ctcatcaccc gtctccgtga
      301 ggtagcgggg gatctcggcg aacgcccgcc cgccggtcgc ctccagcgcg tgggtcctga
      361 tgatctcggc gaactccttc gcctcgcctc ccgtcctgat ctcctggccc ggctcgaggt
      421 ccactccctc gatctcagcc gcgtcgggcg tggcggtgat gttctcctgg gcgatcgtgt
      481 cgcgcacctc gaggaagccc aaggttccga gcaccgcggc cccgatcccg aagaggatca
      541 gcacgatgct ggcgaagtag cccccgaacc gaaagagcct ggtcatggtc actcccttcc
      601 gtttgcaggc aacgtagata ctgccagccg atcgggtcag ggtcgaacgg ccccccgagg
      661 caggggagga agccccgggt ccttgacccg ctgcccgttc cggcgcagag tcggcgatgg
      721 tgccgtcaga agagggggag ccatggtggt caccgtggtc gccgcgcacg tggccgaaga
      781 gcgcggctcg gagttccggc attcgttcgc gggcgcgacc gcggacctgc ctccgggcat
      841 cgtccagacc ttcctcctgc gcgccccgga gagtcacgag tggcggatcc tcaccgtctg
      901 gaggagccgc gaggagctgg aggcctaccg ggccgccgtg gaggtcccgg aggcgatccg
      961 gatgttccgg gccgtgggat cggagcccgt gatgggggag ttcgacgtgc tcgagcaggc
     1021 cgtggagacg gcgggggcct aggccgagcg ctcgggcttg cccaccgcca cgctcgcagc
     1081 gtgcccgttg gtccgcggat ggcaggcctc cgccatcgag gccagcgcgt ccacgtctgg
     1141 ccggaggcgg tccggccaga gcccccgctc ggcgaggaga tcgaggaacg cctcgaccgc
     1201 gacggggtcg aagaggtggc ctgcggcccc ggcgatgtac gcgagcgccc tgtccgccgg
     1261 ccacgccgga cggtaggagc gatcggacgt cagcgcgtcc cagacgtccg ccaccgtggc
     1321 gatgcgagcg gcgatcggga tctcgtcccc cgtcagccgg tcagggtagc cgctgccgtc
     1381 ccaccgctcg tggtggtggc ggatcacggc gagcgaatcg tggagggagg gcgcgcggcc
     1441 ggccatctcg cccccgatga cggggtgctg ctcgatccgg gtccagtcct cgcgggtcag
     1501 ccggtcccgc ttgtgcagca cgtcgtccgg cacgccgatc ttcccgatgt cgtggaggaa
     1561 cgctccctgg gccaggccgc ggagctcgtc ggcgcccatg tccatccgga gcccgatccg
     1621 catcgcgacc tcggccacgc gcgcggcgtg gcctcgcatg tggctgtcct tcgcctcgac
     1681 cgcgccgacc agcgcgtcca gggcctcggg gtggccccgg gagacgagct ggatcgggtc
     1741 cctgatcgag accgtggcca gcgcctcgcg cacgctccgc gtctgccgtg actgcgtcgt
     1801 cacggaccag gccgcggccg cgaaccccgc cagcagatac acgtggtagt cccaccagga
     1861 gacgcgccag aggtgcccca cctccagtgc gaccgcggcg ttggccgaga gccacgccgc
     1921 cgacatcagc ccgagctgga cccggtccct ccccagacgc cagcgatgcc agtggacggc
     1981 ccccacgccg agcagcgtca cggtggtgcc cgcgatgagg acccacctgg cggcctcctc
     2041 gccggggatc ggacgcaggc ccgagagcgc gtgggggcga gcgatgagga cgaccgagaa
     2101 ggcggagagc agggccgcgg cgcccaggag cgagcccctg gggaagcgtc cgacgagtcg
     2161 cttggtggcc cgctccggag cggcggccag ggcgaggcag gcggcgaagc cggccagggc
     2221 caggaccggg aaacggatca cccagaagct gaagccgcgc cccccgatcc cgggagtggt
     2281 catcccgtgg gcgaacatca ggaggccgat cgccaggcag cccatggcca ggagcaccgg
     2341 cgcggcctcc ctcgccttgg cggcggagat cgccgtgagg agcgccacga tcagggcgca
     2401 gagggcgatc gcgctgacga ccacgatgtg gaagcggggc gactcgaaga tgggatcgag
     2461 ggtggggacg gtccggagga tcagggtgat ccccagggga aagaggagga ggctcgcgag
     2521 cacgacggcg ccccgtcccc gagccccacg caggccacga cggagcgcgc gcacggcgcg
     2581 ctcccacctg gggtgcgcct tgaggcccat gccatcgaac gtcggcccct gggctcccgg
     2641 acttgagctc gtcagctgcg cgcggccagc cccgcggcga acgccagcag cgtcgcggcg
     2701 gccgcaaggg tcgtgacgtc cgccacgtca accgtcgggt cgatctcgac gaggtccacg
     2761 gccctgacct ttgggtggcg tcccgcgagg cgagccgcgc ggcggatctc ccacggggcc
     2821 agcccgcccg gcctcgatcc cggggtcgcc ggggcgaacc cccggtcgag gacgtccacg
     2881 tccacgtcca ggtagatcga gtcggcgcgg gcgtcgagat gctccagcgc ctcggtcagc
     2941 acggccccga tcccacgact gcgcacctcc cccgcgagca cgaaggtgat gcccgcctcc
     3001 tgggcgacct cggcgtgcat gggcgagttg gcgaagggct ggatcccgat ctgcacgacg
     3061 ttctcgcccg ggagcccgtc ctcgaggagg gcccgcacgg gattgccgtt gcgcatcccc
     3121 ccgtcggttt ccctgaggtc gaagtgggcg tcgagggtca agagccccaa ccggtcgagg
     3181 gggacgccca gcccgtggac cccgggcctg gtgacgctgt tgtcgccccc cagcaggacg
     3241 agggcgtgcg cgtcgagccc ctgcatcgcg gcgaccaccg agtccagagc gtcgtccggc
     3301 gccacgtcgg ccagcgggag gtcgccgtgg tcctcggccg cgaccacctc gatgtcaccc
     3361 ctcgtctcgc catcccaggt ggagtaccgg ccgagcgcct cgcggatcgc ggcgggggcc
     3421 aggtcggcgc ggctgggcga gatcgagccg aggtgcagcg ggacgccgag gacggccagc
     3481 ctccgccgtg gatgcggcgc gtgggagcct gccagccatt cgctcgctcg aggccagtgc
     3541 ggatcctcgc tcatgagccc tcctcgtcca tggggatcct gatgccgcgc tcccgggcca
     3601 cctcgatggc ccgctcgtag cccgcgtcgg cgtgccggac cacgcccgtg cccgggtccg
     3661 tggtgagaac tcgttccagg cgcagcgccg cctcgtcgct gccgtcggcc accaccacca
     3721 tccccgcgtg gatcgacgag ccgatcccca cgccaccgcc gtgatggacg ctcacccagt
     3781 gcgcgccggc ggccgtgttg acgagggcgt tgaggatcgg ccagtcggcg atcgcgtcgg
     3841 agccgtcggc catcccctcc gtctcgcggt tcggcga
//
LOCUS       DASUTW010000109         6153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_679295, whole genome shotgun sequence.
ACCESSION   DASUTW010000109 DASUTW010000000
VERSION     DASUTW010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679295"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1070
                     /locus_tag="VF058_03455"
     CDS             90..1070
                     /locus_tag="VF058_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6207394.1"
                     /translation="MRIFVVGATGALGRRLVPLLVEGGHAVIGTTRTPDKVEGLMAMG
                     AAPVVLDLLERDAVREALFRTEPEVVVHQATALAGFTDFRKFDQGFAATNRLRTEGTD
                     NLIAAMRGLGIRRLVAQSYAGWPHERVGGPVKTEEDPLDEDPPEAVRRTLGAILHLER
                     AVLDTEGIDGIALRYGGFYGPGTSLGEGGFQLEAVRRRRFPIVGRGTGITSFIHIDDA
                     ATGTLAAIESHRTGLYNIVDDDPAPVAEWLPILADAIDAKPPRRVPVWMARLFAGEHG
                     VVMMTEARGASNAKAKRELGWRPGYPAWRDGFRRGLGGLPRAAAAEAGEC"
     gene            1227..1469
                     /locus_tag="VF058_03460"
     CDS             1227..1469
                     /locus_tag="VF058_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207395.1"
                     /translation="MKKFLIGAAMLVTVVAALRRFGPGLGRLAMRKCEEMLARMPEDF
                     PPKRMMHGIEEIRGQNARILHHLEEGTSLTAARGRR"
     gene            1475..1720
                     /locus_tag="VF058_03465"
     CDS             1475..1720
                     /locus_tag="VF058_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX6207396.1"
                     /translation="MMMGDGWGGWIWGALMMLLFWGGLIALAVFLVRGSGSRPSQGEQ
                     RRREPEARDILAERFARGEISEDEFEERRRVLDRSFS"
     gene            1725..2951
                     /locus_tag="VF058_03470"
     CDS             1725..2951
                     /locus_tag="VF058_03470"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6207397.1"
                     /translation="MTSSEVARVPGLLSLEELATQAGVDIGFAGRLIELGALERRTGY
                     GPSDVRRVQLLRSWEAAGLSIDAVVGLVRSGELPVSWLDPPVATQVERLDLTVGELCD
                     KEDVPIGLVQALQEALGFAPPGARDHVRESDRELVSLVRMFLAEGAAQAPTLRLVRVY
                     ADSLRRIAKAESELYESEIERRSRRSGRSEQQLLDRAARLGDRVIPSLERVLVHIYRR
                     QREHVWMEHCVTHAEAAMERAGLHHGIRRPPAICFVDLTGYTRITEERGDEVAAELAA
                     SLATLVEDISRRHGGRPIRWLGDGGMFLFKEPEAAVWSGLDMVESAPRAGLPPMHIGI
                     HTGPVVFQDGDVYGRTVNLASRIASYAEAGQVLASDETLGHSSDRGLRFASLGPVRLK
                     GISRPVTLHRAARALE"
     gene            complement(2969..3973)
                     /locus_tag="VF058_03475"
     CDS             complement(2969..3973)
                     /locus_tag="VF058_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX6207398.1"
                     /translation="MAGRRTNLALLLLLALALATGGLAFAIGAAWNRWIVLAHGAAGL
                     ALLTLAPWKSVVARRGFRRARRDSWTSVLLTVLVALAILFGVLHAVGVVTVGPLTSMQ
                     AHVGAALASVPFALWHVVARSVRPRRTDLSRRTLLRAGAVAGAGGFAWAAVEGGMRLA
                     SLPGGDRRFTGSHETGSFDPEEMPVTQWLNDSVPEIDARSWRLVVRAQDREEAWGYEE
                     LAAFGDRLRATLDCTGGWFAVQNWEGVRLSQLIPQGQEGRSIHVASATGYGRRFPLAD
                     ADRLLLAIRVGGEPLSAGHGFPARIVAPGRRGFWWVKWVTRIEVSAVPWWWQPPFPLA
                     "
     gene            4070..5284
                     /locus_tag="VF058_03480"
     CDS             4070..5284
                     /locus_tag="VF058_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HEX6207399.1"
                     /translation="MKLEERFQDEAGALAERTTAFLSDIIAIPSLSRREEDVARRIGD
                     EMTDLGYDEVMIDGFGSVIGRIGDGPLGIVYDSHIDTVDVGERSSWKADPFTPRIEDG
                     VIYGRGASDNKAATATMVYGGALIKRLGIDTSRFTVYVVGTVQEEDCDGLALEYVLTE
                     SIPRPDVVVLGEATELDVYRGHRGRVELAVHTRGRAAHASAPERGINAIYRMAPIIRQ
                     IEELNERLADDPFLGKGTIVLSKIDCRTPSLNAVPDGCSVYMDRRLTVGETLETARKE
                     LEELPAVQEAGAEVEVLRYEGESYTGLTLSTEKYFPTWVVPEDDPAVRAGVEAGERAL
                     GRTPAVGRWIFSTNGVSSMGKLGITTIGFGPAEEKYAHSADDQCPLDHLAPAVAWYGA
                     FPESYLAATGSS"
     gene            5301..>6153
                     /locus_tag="VF058_03485"
     CDS             5301..>6153
                     /locus_tag="VF058_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX6207400.1"
                     /translation="MTSVSPNEAAPLEFVLNGRPVSVEVDEDTMLLSVLREDLGLTSM
                     KDGCAPEGSCGACSVIVDGKAVVSCAQRATRAAGKEVITLEGLPQERRDEWAECFWAS
                     GASQCGFCSPGLVMKSEAMLAKNPEPSREEISRAIAGNLCRCTGYVKIIDAIEHAAGT
                     RRGEPVPEVDRSGRVGSRTGRYEGRELALGEKPFVNDMRVPGMLHGAIRFSDHPRARV
                     LRIDTSRAEAHPGVVAVATAGDVPGERRQGLITKDWRQLVAEGEEVSCVGDVLAVVAA
                     ASRAEARE"
BASE COUNT          949 a         2036 c         2302 g          866 t
ORIGIN      
        1 ccaccgtgac atttcgggtc gggtggtgtc acggtggggg acgccgcctc gtcctggatc
       61 ccgatggcac acgacgagag gagcgtgaca tgaggatctt cgtggtgggt gcgacggggg
      121 ccctcgggcg gcggctcgtg ccgctgctcg tcgagggggg ccacgcggtg atcggtacca
      181 cccgaacgcc cgacaaggtc gaggggctca tggccatggg ggccgcccct gtcgttctcg
      241 acctgctcga gcgggacgcc gtccgggagg cgctcttccg gaccgagccc gaggtggtgg
      301 tccaccaggc gaccgcgctc gccggcttca ccgacttccg gaagttcgac caggggttcg
      361 cggccaccaa ccggctgcgc accgagggga ccgacaacct catcgcggca atgagggggc
      421 tcgggatccg tcgactcgtc gcccagagct acgcggggtg gcctcacgag cgggtcggtg
      481 ggcccgtgaa gaccgaggaa gatccgctcg acgaggatcc tccggaagcc gtccggcgga
      541 cgctgggggc gatcctccac ctggagaggg ccgtgctcga cacggagggc atcgacggga
      601 tcgccctcag gtacgggggg ttctatggac cgggaacgtc gctcggggag ggtggcttcc
      661 agctggaggc ggttcgccgc cgacgcttcc ccatcgtcgg cagggggacg ggcatcacgt
      721 cgttcatcca catcgacgat gcggccacgg ggacgctcgc cgcgatcgag agccaccgga
      781 cggggctcta caacatcgtc gacgacgatc cggcgccggt cgccgaatgg ctgccgatcc
      841 tggccgacgc gatcgacgcc aagccgccca ggcgcgtacc ggtctggatg gcccggctct
      901 tcgccgggga gcacggggtc gtcatgatga ccgaggcgcg cggggcctcc aacgcgaagg
      961 ccaagaggga gctcgggtgg aggcctgggt accccgcctg gcgcgacggg ttccgccggg
     1021 gcctgggagg ccttcctcgg gctgcggccg ctgaggccgg cgagtgctga tcgtcgtcgc
     1081 caccgaggcg tcgcttcctc ccataccgca aggtcccgcg tgccacggga ccttcggccc
     1141 tttgcgaccg aaggcgacag gcacacgttg acgtggacgc gagccaccgg tggacgggtg
     1201 ccacgggtga cggtgaggag gaaccgatga agaagttcct gatcggtgcc gcgatgctcg
     1261 tgacggtcgt ggcggcgcta cgacgcttcg ggccggggct gggcaggctc gccatgcgga
     1321 agtgcgagga gatgctcgct cgcatgccgg aggacttccc accgaagcgc atgatgcacg
     1381 gcatcgagga gatccgcggg cagaacgctc gcatcctgca tcacctcgag gaaggaacgt
     1441 cgttgaccgc agctcgtgga cggaggtgag gacgatgatg atgggagacg gttggggagg
     1501 ctggatctgg ggcgccttga tgatgctgct cttctggggt ggcctcatcg ccctggcggt
     1561 cttcctcgtg cgcggctccg gctcccgccc ctctcaggga gagcagaggc ggagagagcc
     1621 cgaggcccgg gacatcctgg cggagcgctt cgcgcgaggg gagatctccg aggacgagtt
     1681 cgaggaacgg aggcgggtcc tcgatcgcag cttctcgtag gacgatgacc tcgtcggagg
     1741 tcgcccgtgt tccgggcctc ctctcgctgg aggagctggc gacccaggca ggggtcgata
     1801 tcgggttcgc ggggcggctg atcgagttgg gggccctgga gcggaggacc ggctacggcc
     1861 cctcagacgt ccgtcgcgtt caactccttc ggagctggga ggccgccggg ctctcgatcg
     1921 acgcggtcgt ggggctcgtt cgttccgggg aactccccgt ttcgtggctc gatcccccgg
     1981 tcgccacaca ggtcgagcgg ctggacctta ccgtaggaga gctctgcgac aaggaagacg
     2041 tgccgatagg cctcgtacag gctcttcagg aggccctggg gttcgcgccg ccaggtgccc
     2101 gcgaccacgt ccgtgagagc gaccgcgagc tggtgagctt ggtccggatg ttcctggcgg
     2161 aaggagctgc ccaggcgccc actctgcgtc tggtgcgcgt gtacgcggac agcctgcgac
     2221 ggatcgcgaa ggccgagtcc gagctctacg aatcggagat cgagcgaagg tcgcgccgat
     2281 cggggcggag cgagcagcag ctcctcgacc gcgccgcccg gttgggtgat cgagtgatcc
     2341 cctccctcga gcgagttctc gtccacatct atcgtcggca acgggagcac gtttggatgg
     2401 agcactgcgt cacccatgcc gaggccgcca tggagcgggc gggtctgcat cacgggatcc
     2461 gccggccgcc ggccatctgc ttcgtggacc tgaccggcta cacgcgcatc accgaggagc
     2521 gcggtgatga ggtcgccgcg gagctggccg ccagcctggc cactctcgtg gaggacatct
     2581 cccgccgcca cggtggccgg ccgatccgct ggctgggcga cggcggcatg ttcctcttca
     2641 aggaaccgga ggcggccgtc tggtccggcc tcgatatggt ggagagtgcg ccgcgcgccg
     2701 ggcttcctcc tatgcacatc ggcatccaca ccggaccggt cgtcttccag gacggcgacg
     2761 tctacgggag aaccgtgaac ctggcctcca ggatcgcgtc ctacgcggag gccgggcagg
     2821 tgctcgcgag cgatgagacc ctgggacatt catcggatcg ggggttgcgt ttcgcgtctc
     2881 tcggccccgt gagactcaag gggatctcga ggccggttac gctccaccga gcggcgcggg
     2941 ccctggaatg agctcggcac gggcgagctc aggcgagcgg gaagggaggc tgccaccacc
     3001 atggcaccgc gctcacctcg atgcgtgtca cccacttcac ccaccagaag ccgcgccgcc
     3061 ccggcgcgac gatccgagcg gggaacccgt ggccggcgct gaggggctcc cctccgaccc
     3121 gtatcgcgag cagcaggcgg tcggcgtcag cgagagggaa ccggcgaccg tagcccgtcg
     3181 cggacgccac gtggatgctg cgcccctcct gcccttgggg gatgagctgc gagagccgga
     3241 ccccctccca gttctgcacc gcgaaccacc caccggtaca gtccagggtc gcccgcagtc
     3301 gatctccgaa ggcggccagc tcctcgtagc cccaggcttc ctcccggtcc tgtgcccgca
     3361 cgaccaggcg ccagctccgg gcgtcgatct cagggaccga gtcgttgagc cactgggtca
     3421 cgggcatctc ctccgggtcg aaggagccgg tctcgtgtga gccggtgaag cgccgatccc
     3481 ccccgggcag ggaggcgagc cgcatgcctc cctccaccgc cgcccaggcg aagcccccgg
     3541 cgcccgccac tgccccggcc cgaagcaggg tcctgcggct gaggtccgtc ctccgcgggc
     3601 gcacgctccg ggccacgacg tgccagagag cgaaggggac cgaggcgagc gccgccccca
     3661 cgtgcgcctg catcgaggtg aggggtccca ccgtgacgac gcccacggcg tggaggacgc
     3721 cgaacaggat ggcgagggcc acgagcaccg tcaggagcac ggaggtccag gaatcgcggc
     3781 gggcccgccg gaagcctcgg cgggcgacga cggacttcca cggtgcgagt gtcaggagcg
     3841 ccaaccccgc ggccccgtgc gccaagacga tccatcggtt ccacgcggcc ccgatggcga
     3901 aggcgaggcc cccggtggcg agtgcgagtg ccagaaggag gaggagggcg agattcgtgc
     3961 gccgtcccgc catcgctcga gtctacccgg gacccccgga ctccggagtt gtgcttgact
     4021 tcgaatcgac cctgggtcga tccgcggccc gggaggggag aggagagaca tgaagctcga
     4081 ggaacgattc caggacgagg cgggggcgct cgccgagcga acgaccgcgt tcctctcgga
     4141 catcatcgcg atccccagcc tctctcgtcg cgaggaggac gtcgcacgac gcatcggcga
     4201 cgagatgacc gacctgggct acgacgaagt catgatcgac ggcttcggaa gcgtcatcgg
     4261 gaggatcgga gacgggcccc tggggatcgt ctacgactcc cacatcgaca cggtggacgt
     4321 gggggagcgg agctcgtgga aggcggaccc gttcaccccg cgcatcgagg acggcgtgat
     4381 ctacgggcgc ggcgcctccg acaacaaggc cgccacggcg acgatggtgt acgggggcgc
     4441 gctcatcaag cgtctcggga tcgacacgtc ccgcttcacc gtctacgtcg tggggacggt
     4501 gcaggaggag gactgcgacg ggctggccct cgagtacgtg ctgaccgaga gtatcccgag
     4561 gcccgacgtc gtcgtgctgg gcgaggccac cgagctggac gtgtaccggg ggcaccgggg
     4621 ccgcgtcgag ctcgccgtcc acacgcgcgg gcgggcggcg cacgcctccg cccccgagcg
     4681 ggggatcaac gcgatctacc ggatggcccc gatcatccgg cagatcgagg agctgaacga
     4741 gcgcctggcc gacgacccgt tcctggggaa ggggaccatc gtcctctcca agatcgactg
     4801 ccggacgccc tcgctcaacg cggtgcccga cggctgcagc gtgtatatgg accgccggct
     4861 caccgtgggg gagaccctgg agaccgcccg gaaggagctg gaggagctcc ccgccgtgca
     4921 ggaggccgga gcggaggtcg aggtccttcg gtacgagggc gagagctaca cgggcctcac
     4981 gctcagcacc gagaagtact tccccacgtg ggtggtgccc gaggacgacc ctgccgtccg
     5041 ggccggcgtc gaggcgggcg agcgcgccct ggggaggacg ccggcggtgg gtcggtggat
     5101 cttctccacg aacggggtct cgagcatggg gaagctcggg atcacgacga tcggcttcgg
     5161 ccccgccgag gagaagtacg cccacagcgc ggacgaccag tgcccgctgg atcatctcgc
     5221 cccggccgtc gcctggtacg gggccttccc ggagagctat ctggccgcga cggggtcctc
     5281 ctgagaggat gggaccggag gtgacgtccg tgtccccgaa cgaagcggcc cccctcgagt
     5341 tcgtcctcaa cggccggccg gtgtccgtgg aggtcgacga ggacacgatg ctgctctcgg
     5401 tgctccggga ggatctgggg ctcacctcga tgaaggacgg gtgcgcgccc gaggggtcct
     5461 gcggcgcgtg cagcgtgatc gtggacggga aggccgtcgt ctcctgcgct cagcgggcca
     5521 cccgggcggc cggcaaggag gtcatcaccc tcgagggcct cccgcaggag cggcgcgacg
     5581 agtgggccga gtgcttctgg gcctccgggg cgtcgcagtg cgggttctgc tccccgggcc
     5641 tcgtcatgaa gtccgaggcg atgctggcga agaaccccga gccctcgagg gaggagatct
     5701 ccagagcgat cgcggggaac ctctgccggt gcaccggcta cgtgaagatc atcgacgcga
     5761 tcgagcacgc ggccgggacg cggaggggcg agccggtccc ggaggtggac cggagcggcc
     5821 gggtgggctc ccgcacaggc cggtacgagg gccgcgagct ggcgctcggc gagaagccct
     5881 tcgtcaacga catgcgcgtg cccgggatgc tccacggggc gatccggttc tcggaccacc
     5941 ccagggcccg tgtgcttcgg atcgacacct cgagggccga ggcccacccg ggggtggtgg
     6001 cggtggccac ggccggggac gtgcccgggg agcggcgaca ggggctcatc acgaaggact
     6061 ggcgccagct cgtggccgag ggcgaggagg tgagctgcgt cggcgacgtc ctggcggtgg
     6121 tggcggccgc ctctcgggcc gaggcccgag agg
//
LOCUS       DASUTW010000110         9532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_420310, whole genome shotgun sequence.
ACCESSION   DASUTW010000110 DASUTW010000000
VERSION     DASUTW010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9532
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420310"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..703)
                     /locus_tag="VF058_03490"
     CDS             complement(122..703)
                     /locus_tag="VF058_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX6207401.1"
                     /translation="MGRLGWLIVAAAASVAVPAAPASAGGGGGGGCPEPLTSGTGTVV
                     EMRDVCFSPTVIRVRPGTRVNFVNRDPFEHNIIGANGRWGEYDDMQLDETFSRTFREP
                     GVFPYACAFHPGMMGSVVVGRGVVVETAFVPPSPEPVETPAAELPLVVPDPPMRVSSL
                     EPAQGWGPAALAPWAVAAAAVGFGVAQRRRRRA"
     gene            782..1174
                     /locus_tag="VF058_03495"
     CDS             782..1174
                     /locus_tag="VF058_03495"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX6207402.1"
                     /translation="MEREPGHWLIDGMNLIGSKPNRWWKDPGRAIRELIDELEDYAER
                     SGEPLTVVFDFRPKGLRVGTSGGIKVRFAEGGSQAADRMIADLVRDHPHPQTLTVVTS
                     DRPLRQIVEAEGARVVGAGTFRGRLEGR"
     gene            1274..4411
                     /locus_tag="VF058_03500"
     CDS             1274..4411
                     /locus_tag="VF058_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX6207403.1"
                     /translation="MRSPRNTLLTVLAASLLVGAIVTPTVAQQGSGGGGPRLQLYEAT
                     VTAEQWTEIEGLGLDIAHVETTPDGVHFQVVMYPNERQALRRRGISLRPVRNEDGLTQ
                     IQAAQEQSIFGFDVWRSYDESGGIEDQLRAIASAPQNRGFAALHKLGESHQGRDILAV
                     RLTQGARGLPVGKRPAVLYQGTTHAREWISTEVDMRLLKWFVAERRAGNPEIQELLET
                     TELWFVPVVNPDGYEYTFTDDRLWRKNLRDNNGDGEITPGDGVDLNRNYPEHWNYDEE
                     GSSSQFASETYRGPTPASEPEVQANIRLFDMLPKLRFAISYHSFGQLLLYTQGWQVQT
                     PSADDPIYVALTGTDDDPAVEDFDPGVGADLYTTNGEFTDWAHGERDVLAWTPELSEG
                     CPGCGFVFPDDEALVQEEFERNLEFALNVARSALDPDDPASHAGIDTEDFYLDVSEID
                     PWKTNHPSSDLAVDISYAGGSSQPVDVLAKRSLGDVTLNYSVNGGATQTAPTSAAPDG
                     EVFGGNNAYNAYYHFLRGEIPGLSVGDSVEYWFAAGSSSSDHQSFDVVEDADADVLIV
                     ANEDRTGAVNIPGYASTDPNVPNYLSSYTDALDAAGHTYDVWDVDAMGREAPDHLGVL
                     SHYDAAIWYMGNNFVTRTAGRGPGNIDRLANDLILESRAYLNEGGKLLYTGQWAGAAE
                     NGLAGTQLYDPVAGETCVVGGALVLDRCQVFSDKNDFLQYYLGAYIYNSDAGTDEAGN
                     PFSVLGVDDPYTGTNWEFNGADSAQNQVHTASFITTSSLLPPGEYPQFASTGPAVWDT
                     GAAGAFEPFDGDWYVYSNMGDISYKRLMRTIDLTGVTAADNPTLTFRFSYDTEPDWDF
                     VFVEAHTVGADDWTTLPDQNGNTNNSTGSSCASGWFELHPWLERYQGADCSGTGTTGE
                     WNAHSGRSAGWEEWEIDLSDYAGSEIEVSISYASDWAVQGLGAWVDHVEVSTEPGVES
                     FETGLGAWNISGPAEGSGPNPNNWERTQSVGFEEGAVAATADTLFFGFGFEGITGASS
                     RAAVMEKSMGYLLGP"
     gene            complement(4525..4992)
                     /locus_tag="VF058_03505"
     CDS             complement(4525..4992)
                     /locus_tag="VF058_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6207404.1"
                     /translation="MAEHVSLRDVTHEDLDVFYQHQLDPEAAGMAAATPRGRGAFRAH
                     WRRILADPTVVTQAVLVDGELAGNVVSFLREGKREVGYWIGKRYWGRGVATAALARFL
                     ELIDERPLHAYVAKHNLASVRVLEKCGFEVVGEDLAFTTIAGEPVEGFILQLS"
     gene            5189..6043
                     /locus_tag="VF058_03510"
     CDS             5189..6043
                     /locus_tag="VF058_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207405.1"
                     /translation="MFLNQNEKATDAQLLNGIDSAGFLRVDATAADSDLLDGMDSGAF
                     QRSYVRTVVVDTAAELIAAVGSASPNTMIQLEPGTFNVGATMLSIPSGVTVQGAGIRT
                     LIVGAGSADPNHAVVAAGGSTEIRSLTILNTGNQPFAVAARIQGNARLFDVNVQAQNG
                     TTESVAIDAVTGGGSAVPLLRQVGAFTLGAPNAESVAMRAGGVNVHVIDSILQAFQGS
                     GGTGTAFRGLSGLHKIERSILFGDTAAVNVTGGGQANVALTRLEGTTFGAVCFDVYDD
                     DFTPVTCA"
     gene            6186..9044
                     /locus_tag="VF058_03515"
     CDS             6186..9044
                     /locus_tag="VF058_03515"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX6207406.1"
                     /translation="MRRAATLLALLALGAATIAVPPLAAAAQPEEEIQPLYKHPPGLG
                     AHEAIASVSAPTLAAFRAEREAARGAAVGGGVPAGPNVRTTNSPGTQAEISMAATGGG
                     GQNIVLAYNGGPNGLGIVSSQNGGQTFQAEQSAPTPAGSNPCCDPSVVADAVSSFFLI
                     QLFRDDGANAPMAGNCTNSLHVSTDGGQTFSNIVSSPFSYPPGGPDFPDQPHIGIDRG
                     NPNNLYVFTRHFTSGINCPQTGGSGTVQGEVVCSNDGGATWSNPFVFPQFTDTAHLDV
                     APDGTAYVVGNGVGTNMGTTRVILWRATATTCPGAGNTPNLAGPTVVADNLTFGNVGI
                     DREFPQPTVLADPTNSNRAYVNFSADSTQGSGDREAFLSRCDFPAATCTALLTVNDNP
                     ADGSDQYFPMACIDPATNNVFFSWNDDRSGQEEIRATTVTNNGTTTGASRRVSEVTWP
                     IINPGGTPDYGDYNENNGACRANHHYAAWSSQVSPPGITPASTDIDVFFTVVNEPPEA
                     DAGGPYMTVEGTDVGLSGSATDPENDTPFTFEWDLDGDGEFDDATGQTPTFTDVGQDG
                     VFPVCLRVTDSIGDAGEDCTTVTVVNVAPSLVGLSQDGPKPEGDAVTVSGTVTDPGWE
                     EMLSVTIDWGDGTPVETVGGILENERPDATLAFSAPHVYGDNGIFSVEVCGHDDDTQT
                     CQTIDVTFTNVPPTAGFDLTGTEIVNGVPTVIGEAGEPVELTGRATDPGSDDLFLRWD
                     FGDGSPIVLVTSLVNPPLPDPPNSPSVQPRDVTDTQSHTYGDACIYEATFSATDDDGG
                     ASGPATLAVLIAGNADRTRPTGWWHRQYMRGGARADFAVATLECYLAIARFGSSVFDE
                     ATALASLAHAADVLKGGGKPQHLSRFDRELLTAWLNLANGAVGFTELVHDADGDGTLD
                     TTFGAAVAAAESVRLGASTPAQVRAQQRILHRINVPAS"
     gene            complement(9095..9451)
                     /locus_tag="VF058_03520"
     CDS             complement(9095..9451)
                     /locus_tag="VF058_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX6207407.1"
                     /translation="MAIEDLAAEQFCYVTSIGRVTGRPHTIEIWFALDGRTLYLLSGG
                     GRASDWVRTLERTPDVTVRLGDRIFPGRARVVTDAEEDERARRLVHDKYQPGYGGDLA
                     GWRRRSLPVALDLDED"
BASE COUNT         1566 a         3395 c         3287 g         1284 t
ORIGIN      
        1 gctcctcgaa cgcgtcgagg tccccgcgcc gcgcgcgggc gaccacctcc ggctcgggct
       61 cgcgcaccgc cgccctccgc tgtcggcctg agtctgacga cgggatggcc tcccgacaag
      121 gtcaggcccg gcggcgtcgc cgctgggcga ccccgaaccc caccgccgcg gcggcgacgg
      181 cccagggagc gagggccgcc gggccccatc cctgcgcggg ctcgaggctg gagacgcgca
      241 tcggggggtc gggaaccacc agaggcaact cggcggcggg cgtctccacg ggctccggcg
      301 agggcggcac gaaggcggtc tccaccacga ccccgcggcc gaccaccacg cttcccatca
      361 tcccgggatg gaacgcgcag gcgtagggga acacgcccgg ctccctgaag gtgcgggaga
      421 aggtctcgtc cagctgcatg tcgtcgtact cgccccagcg accgttcgcc ccgatgatgt
      481 tgtgctcgaa cggatcgcgg ttcacgaagt tcaccctcgt cccgggccgg accctgatca
      541 cagtcgggga gaagcagacg tcgcgcatct cgaccacggt tccggtaccg ctcgtcagcg
      601 gctcgggaca gcctccgccc ccacccccgc cggccgacgc cggcgccgcc ggcacggcca
      661 cgctcgccgc cgcagcgacg atgagccatc cgagccttcc catcgatcgc ctcctcgttc
      721 gctcatcccc tcagacgcac gcgggcccat cccggttcgc cgggacggct acggtgaacg
      781 catggagcgc gagccgggac actggctgat cgacgggatg aacctgatcg gctccaagcc
      841 gaaccgctgg tggaaggacc cggggcgggc gatccgcgag ctgatcgatg agctcgagga
      901 ctacgccgaa cgatcgggcg agccgctcac ggtcgtcttc gacttccggc cgaaggggct
      961 gagggtgggg acctcggggg ggatcaaggt gcgattcgca gagggcggat cccaggccgc
     1021 cgaccggatg atcgccgacc tggtccgcga ccacccccat ccgcagacct tgaccgtggt
     1081 gacgtcggac cggccgctcc ggcagatcgt cgaggcggaa ggcgccaggg tggtgggggc
     1141 gggaaccttc cgcggccgcc tggagggtcg ctgaggagcc ccgaggatgc gatttgacct
     1201 gccgatcaga ttgggtctat ggtgcgaccc cgccaacagg gattcgtccg tctgcagaca
     1261 agggagattc cgtatgcgga gcccacgcaa caccctcctc accgtcctcg ccgcgtcgct
     1321 gctcgtcggc gcgatcgtca ccccgaccgt cgcgcagcag ggctcgggcg ggggcgggcc
     1381 gcgcctccag ctgtacgagg ccaccgtgac cgccgagcag tggaccgaga tcgagggcct
     1441 cgggctcgac atcgcgcacg tcgagaccac cccggacggc gtccacttcc aggtggtgat
     1501 gtatccgaac gagcggcagg cactccggcg gcggggcatc agcctgcgcc ccgtccgcaa
     1561 cgaggacggc ctgacgcaga tccaggccgc gcaggagcag tccattttcg gcttcgacgt
     1621 gtggcgctcc tacgacgagt caggcggcat cgaggaccag ctccgagcga tcgcctcggc
     1681 gcctcagaac cgcgggttcg ccgccctcca caagctcggc gagtcccatc aggggcggga
     1741 catcctcgcc gtgcgcctca cccagggcgc ccgtggcctc cccgtgggga agcggcccgc
     1801 ggtcctctac cagggcacca cccacgcccg tgagtggatc agcaccgagg tggacatgcg
     1861 cctcctcaag tggttcgtgg ccgagaggag ggccgggaac cccgagatcc aggagctgct
     1921 ggagacgacc gagctctggt tcgtcccggt ggtcaacccg gacggctacg agtacacgtt
     1981 cacggacgac cgtctgtggc ggaaaaacct ccgcgacaac aacggggacg gcgagatcac
     2041 gccgggcgac ggcgtcgacc tcaatcggaa ctatcccgag cactggaact acgacgagga
     2101 gggctcctcc tcgcagttcg ccagcgagac ctaccgcggc ccgaccccgg cgtcggagcc
     2161 cgaggtccag gcgaacatcc ggctgttcga catgctgccg aagctgcggt tcgcgatcag
     2221 ctatcactcg ttcggccagc tcctgctgta cacgcagggc tggcaggtgc agactccgtc
     2281 ggccgacgac ccgatctacg tggccctgac ggggaccgac gacgacccgg cggtcgagga
     2341 cttcgacccc ggcgtcggcg cggacctcta cacgacgaac ggggagttca ccgactgggc
     2401 ccacggcgag cgcgacgtgc tcgcctggac cccggagctg tccgaggggt gcccgggctg
     2461 tgggttcgtc ttcccggacg acgaggccct cgtgcaggag gagttcgagc ggaacctcga
     2521 gttcgcgctg aacgtggcgc gctcggcgct cgaccccgac gacccggcct cgcacgcagg
     2581 gatcgatacc gaggacttct acctcgacgt gtcggagatc gacccgtgga agacgaacca
     2641 cccgtcgtcc gacctggcgg tggacatctc ctacgcgggc gggtcctcgc agccggtgga
     2701 cgtcctggcg aagcggtcgc tgggcgacgt gacgctcaac tacagcgtca acggaggcgc
     2761 cacgcagacg gcgccgacga gcgcggcgcc cgacggtgag gtgtttggag gcaacaacgc
     2821 ctacaacgcc tactaccact tcctccgtgg cgagataccc ggcctgtcgg tcggcgactc
     2881 ggtggagtac tggttcgccg ccgggtcgtc atcgagcgac caccagtcgt tcgacgtggt
     2941 cgaggacgcc gacgccgacg tcctgatcgt cgccaacgag gaccggacgg gggccgtgaa
     3001 catccccgga tacgcgagca ccgacccgaa cgtcccgaac tacctctcgt cgtacacaga
     3061 cgcgctggac gcggccggcc acacgtacga cgtgtgggac gtggacgcga tgggccggga
     3121 ggccccggat cacctcggcg tgctgagcca ctacgacgcg gcgatctggt acatggggaa
     3181 caacttcgtg acccggaccg ccggccgcgg ccccggcaac atcgaccgac tggccaacga
     3241 cctgatcctc gagtcgaggg cctacctcaa cgagggcggg aagctcctgt acacgggcca
     3301 gtgggcaggc gcggcggaga acggcctcgc cggcacccag ctctacgacc cggtggccgg
     3361 cgagacctgc gtggtcggcg gggcgctggt gctggaccgg tgccaggtgt tctcggacaa
     3421 gaacgacttc ctccagtact acctcggcgc ctacatctac aacagcgatg cgggcaccga
     3481 cgaggccggg aacccgttct cggtgctggg ggtggacgat ccgtacaccg gcacgaactg
     3541 ggagttcaac ggggcggaca gcgcccagaa ccaggtccac acggcatcgt tcatcacgac
     3601 gagcagcctc ctgccgcccg gcgagtaccc gcagttcgcc agcacggggc cggcggtgtg
     3661 ggacacggga gcagcgggcg cgttcgagcc cttcgacgga gactggtacg tctactcgaa
     3721 catgggggac atcagctaca agcgcctgat gaggacgatc gacctcacgg gcgtgacggc
     3781 ggcggacaac cccacgctga cgttccggtt ctcctacgac acggagccgg attgggactt
     3841 cgtcttcgtg gaggcgcaca cggtgggggc ggacgactgg accaccctgc cggaccagaa
     3901 cggcaacacc aacaacagca ccgggtcgag ctgcgcgagc ggctggttcg agctgcaccc
     3961 ctggctcgag cggtaccagg gggcggactg ctccggcacc gggaccaccg gtgagtggaa
     4021 cgcgcactcc ggccgctcgg ccggctggga ggagtgggag atcgacctct ccgactacgc
     4081 cggcagcgag atcgaggtct cgatctccta cgcgagcgac tgggccgtcc aaggactcgg
     4141 cgcgtgggtg gaccacgtgg aggtctcgac cgagcccggt gtggagtcct tcgagaccgg
     4201 cctcggggcc tggaacatct cgggcccggc cgagggcagc gggcccaacc ccaacaactg
     4261 ggagcggacc cagtccgtcg gattcgagga gggcgccgtc gcggcgaccg ccgacaccct
     4321 gttcttcggg ttcgggttcg agggcatcac gggtgcctcg tccagggccg ccgtgatgga
     4381 gaagagcatg gggtacctgc tcgggccgta gagcccagcc ggtacgaggt gaggggccgg
     4441 cggcccgttc gggccgccgg cccctcaccc tttgtcagcc aagcgtcgtc gcctggcacg
     4501 gaacgcctct cgcgggggct tcgcctagct cagctggagg atgaacccct ccacgggctc
     4561 cccggcgatc gtggtgaagg ccagatcctc gcccacgacc tcgaagccgc acttctcgag
     4621 caccctgacc gaggcgaggt tgtgcttggc cacgtaagcg tgcagagggc gctcgtcgat
     4681 cagctccagg aaccgggcca gggcagccgt ggccaccccc ctcccccagt agcgcttccc
     4741 gatccagtag ccgacctcgc gcttcccctc ccgcaggaag ctgacgacgt tccccgcgag
     4801 ctccccgtcc acgagcacgg cctgcgtgac cacggtgggg tcggcgagga tccgtcgcca
     4861 gtgggccctg aacgcgccgc gcccccgtgg cgtggccgcc gccatcccgg ccgcctccgg
     4921 atccagctgg tgctggtaga agacgtccag gtcctcgtgg gtcacgtcgc gcagggacac
     4981 gtgctcggcc acggcgcata ccctaccgac cttcagccct cccgaagcct ctggaggatg
     5041 atgagcgcca ccgatccaag ggaggcggag atgcggaggc acacccagac gatcctcgtg
     5101 gccgtgctcg cggtcctcgt ggtagccgtc gccccggccg gcgccacctc aacaagaagc
     5161 tcaagcacct gatcaagcac ctcgacccgt gttcctgaac cagaacgaga aggcgacgga
     5221 cgcgcagctg ctgaacggga tcgactccgc ggggttcctc cgggtcgatg ccaccgccgc
     5281 ggactcggac ctgctagacg ggatggactc cggcgcgttc cagcgctcgt acgtcaggac
     5341 ggtcgtcgtg gacacggccg ccgagctgat cgccgccgtg gggtcggcga gcccaaacac
     5401 gatgatccag ctcgagcccg gcacgttcaa cgtgggcgcg acgatgctct ccatcccgag
     5461 cggcgtgacc gtccagggcg ccggcatccg gacgctgatc gtgggggccg gttccgcgga
     5521 tccgaaccat gccgtggtgg cggccggcgg cagcaccgag atccgctctc tgacgatcct
     5581 gaacaccggc aatcagccgt tcgccgtggc cgctcgcatc cagggcaacg ctcggctctt
     5641 cgacgtcaac gtgcaggccc agaacgggac gaccgagtcg gtggcgatcg acgccgtcac
     5701 cggaggaggt tccgcggtgc cgctgcttcg ccaggtgggc gctttcacgc tcggcgctcc
     5761 caacgccgag tcggtcgcca tgcgggcggg cggcgtgaac gtccatgtga tcgactcgat
     5821 cctccaggcc ttccaggggt ccgggggcac ggggacggcc ttccgcgggc tgagcggcct
     5881 ccacaagatc gagcgatcga tccttttcgg agacaccgcc gcggtgaacg tgacgggcgg
     5941 cggtcaggcg aacgtcgcgc tcacgcgcct ggaggggacc accttcggag cggtctgctt
     6001 cgacgtctac gacgacgact tcacgccggt cacctgcgcg tgacgcggga ggggcgcgtc
     6061 ccccctccca ccgacggccg aacggccgtc ttgaccggtc cgcggagcaa gcgtaggacg
     6121 ggacccgggc cggcgagcct cgtgcggacg aggccggcgg ctcgtggacg cggagggagg
     6181 accccatgcg acgtgccgcc accctgctcg ctctcctcgc gctgggagct gcgacgatcg
     6241 ccgtgccgcc ccttgcggcg gcggcccagc ccgaagagga gatccaaccg ctctacaagc
     6301 atccccccgg cctcggcgcc cacgaggcga tcgccagcgt gagcgccccg accctggccg
     6361 ccttccgggc agagcgcgag gccgctcgcg gcgcagcggt cggcggcggc gtgccggcgg
     6421 ggcccaacgt ccgcacgacg aactcgcccg ggacgcaggc cgagatctcg atggccgcca
     6481 cgggaggcgg cggccagaac atcgttctgg cctacaacgg cgggccgaac ggcctgggga
     6541 tcgtctcgtc gcagaacggc gggcaaacct tccaggccga gcagagcgca ccgacgccgg
     6601 cgggaagcaa tccgtgctgc gacccctcgg tggtggccga cgctgtcagc agcttcttcc
     6661 tgatccagct gttccgggac gacggagcga acgcgcccat ggcgggcaac tgcacgaact
     6721 cgctgcacgt gtccacggac ggcgggcaga cgttctcgaa catcgtcagc tctccgttct
     6781 cctacccgcc cggaggcccc gacttcccgg atcagcccca catcgggatc gaccgaggga
     6841 accccaacaa cctctacgtg ttcacgcgcc acttcacctc ggggatcaac tgcccccaga
     6901 cgggcgggag cgggaccgtg cagggcgagg tggtctgctc gaacgacggc ggagcgacct
     6961 ggagcaaccc gttcgtcttc ccgcagttca ccgacacggc ccacctcgac gtggcgcccg
     7021 acggcaccgc ctacgtcgtg ggcaacggcg tcgggacgaa catgggaacg acgagagtga
     7081 tcctgtggcg agcgacggca acgacgtgcc cgggagccgg gaacacgccg aacctcgccg
     7141 gccccacggt ggtcgccgac aacctcacct tcggcaacgt ggggatcgat cgggagttcc
     7201 cgcagccgac ggtgctcgcc gacccgacga acagcaaccg cgcgtacgtg aacttctcgg
     7261 ccgactcgac gcagggtagc ggcgaccgcg aggccttcct ctcgcggtgc gacttccccg
     7321 cggcgacctg cacggccctg ctcacggtga acgacaaccc ggccgacggc tccgatcagt
     7381 acttccccat ggcctgcatc gaccccgcga cgaacaacgt gttcttctcg tggaacgacg
     7441 accgctcggg ccaagaagag atccgggcca ccaccgtcac caacaacggg acgacgacgg
     7501 gggccagccg ccgggtctcc gaggtgacct ggcccatcat caatcctggg ggaacgccgg
     7561 actacggcga ctacaacgag aacaacggcg cgtgtcgagc gaaccaccac tacgcggcgt
     7621 ggagctcgca ggtcagcccg ccgggcatca caccggcctc cacggacatc gacgtgttct
     7681 tcaccgtcgt caacgagccg ccggaggcgg acgcgggcgg tccctacatg acggtcgagg
     7741 ggacggacgt cggcctctcc gggagcgcca cggaccccga gaacgacacg cctttcacgt
     7801 tcgagtggga cctcgacgga gacggggagt tcgacgacgc gaccgggcag acgccgacct
     7861 tcacggacgt gggccaggac ggggtgttcc ccgtctgcct gcgggtgacc gactcgatcg
     7921 gggatgcggg ggaggactgc acgaccgtca cggtggtgaa cgtggctccc tcgctggtgg
     7981 ggctctcaca ggacgggccg aagcccgagg gcgacgcggt cacggtctcg gggacggtga
     8041 ccgaccccgg gtgggaggag atgctcagcg tcacgatcga ctggggggac ggcaccccgg
     8101 tggagaccgt cggggggatc ctggagaacg agcgacccga cgcgacgctg gccttctcgg
     8161 cgccgcacgt gtacggggac aacggcatct tctcggtgga ggtctgcggt cacgacgacg
     8221 acacgcagac gtgccagacg atcgacgtga ccttcaccaa cgtgccgccg acggccggct
     8281 tcgacctcac ggggaccgag atcgtgaacg gcgtccccac ggtgatcggt gaggcgggcg
     8341 agcctgtgga gctcaccgga cgagccaccg acccggggtc ggacgatctc ttcctgcggt
     8401 gggacttcgg ggacggatcg ccgatcgtcc tcgtcacctc gctggtgaac ccgcccctcc
     8461 ccgacccgcc gaacagcccg tcggtccaac cccgcgacgt caccgacacc cagagccaca
     8521 cgtacgggga cgcgtgcatc tacgaggcga cgttctccgc gacggacgat gacggggggg
     8581 cgagcgggcc ggccacgctc gccgtcctca tcgcggggaa cgcggatcgg acccggccca
     8641 cgggctggtg gcaccgccag tacatgcggg gcggggcgcg ggcggacttc gccgtggcca
     8701 cgctcgagtg ctacctcgcc atcgcgcggt tcggcagctc cgtcttcgac gaggccacgg
     8761 cgctggcgag cctggcccac gccgcggacg tcctcaaggg tgggggcaag cctcagcacc
     8821 tcagccgatt cgaccgagag ctgctcaccg cctggctgaa cctggcgaac ggcgccgtgg
     8881 gcttcaccga gctggtccac gacgcggacg gtgacgggac cctcgacacg acgttcgggg
     8941 cggcggtggc cgccgccgag agcgtccgcc tcggcgcctc gacccccgct caggtcaggg
     9001 cccagcagcg gatcctgcac cgcatcaacg tgccggcttc gtgacgaggt gccggtgctc
     9061 ccgccccctc cggggggcgg gagcaccggc acgatcagtc ctcatcgaga tcgagcgcca
     9121 ccggcaatga gcgtcgccgc cacccggcca ggtccccccc gtagcccggc tggtacttgt
     9181 cgtggacgag gcggcgagct cgctcgtcct cctcggcgtc cgtgaccacc cgggcgcgac
     9241 cggggaagat cctgtccccc aaccgaaccg tcacgtcggg cgttcgctcc agggtccgca
     9301 cccaatcgga ggcccgcccg ccccccgaca ggaggtagag cgtcctcccg tcgagcgcga
     9361 accagatctc gatggtgtgc gggcggcccg tgacgcgtcc gatggaggtg acgtagcaga
     9421 actgttcggc cgcgagatcc tcgatcgcca tctcgagccc accttagccg cgtgaacctc
     9481 cccgcaccgt ggagcgtcta cctctcgggg gacggccatg gcgtgtgacg ac
//
LOCUS       DASUTW010000111        30856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_551205, whole genome shotgun sequence.
ACCESSION   DASUTW010000111 DASUTW010000000
VERSION     DASUTW010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 30856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30856
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551205"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VF058_03525"
     CDS             <1..668
                     /locus_tag="VF058_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207408.1"
                     /translation="EARIASMEAQLAGTRADLEQQARAAETRATQAEAELAAARAELA
                     ARPETPTPVDASEQISAIIQASERAATRIIDVAGQAHQEQVEQLDRMRAQVREEADRM
                     VSWREQLGPIVHSIRSSIDRARARIDDIPDRIREALNPLGEAIDSVNDQLTSLVEEVA
                     EPATSEDERGPGGAEDDDRSEPERPDDSSAPIRAIRLDEPEAARTGDATDDPGTHPGW
                     HRR"
     gene            complement(672..1646)
                     /locus_tag="VF058_03530"
     CDS             complement(672..1646)
                     /locus_tag="VF058_03530"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207409.1"
                     /translation="MTFLVALIGGIVLTPLARAVGLLTGVVDRPDGGELKIHRRPIPL
                     TGGLAVVASMAASLWILGDLPSGWILGAVALALVVGVVDDAFPLPAAVRFLAQVGSGL
                     LLMGAGLRLEPLGIFAAIGVVLLVPATTNAVNLIDGQDGLAGSLGAVAALGLALLLAR
                     PGGDPGAALALATAGALIGFLVWNRPPARIFLGNGGAYAVGVLLAVPAATLSTDGWRG
                     LLAAALCLGVFAFELLFTVARRMVFRRPMAGGDRAHSYDLVAARSGDRLRTTIAFGAL
                     AILAGGGALVVGLTPLSFGVAVFTLACAAAALLGRGLWRDLRGDPRVA"
     gene            complement(1643..3415)
                     /locus_tag="VF058_03535"
     CDS             complement(1643..3415)
                     /locus_tag="VF058_03535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6207410.1"
                     /translation="MSRIAHLTTVDLTLRFLLLGQLRRLRDEGHEVVGISAPGPWVAD
                     LEREGIRHVPWPHATRSWDPRGDLRALRELVGILRRESFDLVHTHNPKPGILGRLAAR
                     AAGVPCVANTVHGLYATPQDRLMKRAAVLSLEWLAAWFSDLELYQSEEDLLWARRRRL
                     VDPPRSVLLGNGTDLSRFDPSKVDRGQIDELRRDLGIRPDEIVVGTIGRMVAEKGYRE
                     LFRAAEVVRLARPKARFLVVGERDPEKADSLSEEEIARAKEHVAFAGWRTDVPELLAA
                     MDMFILPSWREGMPRSAIEAAAMGRPLVLTDIRGCREVVRHDEEGLLVPPRAPGRLAE
                     AIVRLIDDPGLRERMGRDARARAIERFDERKVVDRVAAETTRLLVRKTRGDGRTWLRP
                     ATSGDAGAMARLHRESMPTAFLPTLGDGFLRQLYEALISDPQGVALVVEREGRVLGFA
                     TGVPSVPRFFRRFALRRGVRAFLSAAPRLASGQVRRRMRETAAYPEASVSLPPAELLS
                     IAVAPGARAKGVGRALAGGVLTALGRRGATEVKVVVGADDEDANRFYERVGFRLAARL
                     EVHAGTASNVWVRDVGAERRPEAT"
     gene            complement(3412..4176)
                     /locus_tag="VF058_03540"
     CDS             complement(3412..4176)
                     /locus_tag="VF058_03540"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6207411.1"
                     /translation="MELLRKGIKTAVLPAGVLRPRRDGDLVVLLYHRVGVGSSEIDLP
                     LELFERQMRTLAEEDRPTGLDEAVSDGSRGGVVVTFDDGTPDFHSHVVPVLVRCRIPA
                     VLYLATGPVEGGNGSLSWAQLEEAVATGLVTVGSHTHSHRALVRVSEREAEDEMRRSK
                     ELIEDRLGVECRHFAYPFAAGSPAADRAARRVFRTAALHAWRTNRKGHTDPHALGRTP
                     VLRSDGRRFFRAKVRGMLDGEALIYRALRRGPWRWS"
     gene            complement(4182..5234)
                     /locus_tag="VF058_03545"
     CDS             complement(4182..5234)
                     /locus_tag="VF058_03545"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX6207412.1"
                     /translation="MATRTEIDRSVGEGGNATPKLRGAVLDVAGVALFAALVGWTILA
                     GTVSGDDPALVSGLVAVSGMGVLVGRVAGIRSGFIIPAALGAAALVLIVRSPDDLVGS
                     GPLGGPFGYANATGAFLAQAAIAGVIIGVSGRWVALRVVGWAAAIGFGTVVFGSGSMA
                     AFALVVALPLVALAVSRWLSPRVAVGLMAGLFATALAATIVVGATYPSDGSAEPGIIE
                     RTVGERRAVLWRDAVDIMAEEPLTGAGIGSFQRLSPTAVDDADARWAHNEFLEVGAET
                     GFPGLILLVLAFAWVFARLWMGPPDSVAALGAAAAAAIGIHASIDYVLHFPAIPIAAA
                     ALIGSTTAWNRRSGGR"
     gene            5390..7474
                     /locus_tag="VF058_03550"
     CDS             5390..7474
                     /locus_tag="VF058_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207413.1"
                     /translation="MPTEDLSRIPEWITVREASFLTDRSLDGLLRLIWDGRLRSTSVT
                     GSPDPRLLLVPTADLVDLGLLEAESPGVGPISIDERRRRAERRIASPFASWGKVAAIL
                     VLFVGAALTGPVRSSIVELFGAHAAKTWQVKRTPPCHSNSHKRKQCQSPSPTPSPSPA
                     PNPSPSPSPEPSPEPTEPVPSPTLEPSPEPSSSRSAPPEGVHTVSGFVSGGFTVPAGE
                     VWSVDSLVETNGSVVVHGTLVMRPGDTLRFVGVDESAFVGGDTAPVATDVGLWVEGHG
                     VLDARGTPKVAWNRTGADPSWSPSDELVVAPTSTGDYGGAGFASFTMGSAVPQVNGHP
                     AEVLNLTRDVNIEGTSGGRAHIRFRSSRPQVIEYVGIRHMGPRQPGEDGYTDDVPGRN
                     PLHFHMMGDASRGSVIRGVVVRDAGAHAFVPHMSHGVTFVDTIAYDVFEAAYWWPPVA
                     EANATGPETHDTLYLRSVAALVKDDPPFRGHRLSGFFLAAGEGNAIRGSVAVGVQGTK
                     NASAFHWPERSTGSTTSGVWDFDDNVAHNNKVDGVFVWQNNTRAHVVDHFITQGSGQA
                     GIEHGAYGNRYVYSDFTLREDRLGVLLHAAGATFRDAQISAPVGVVFGKHRFDSPETL
                     FSRVAFQVSGKAVVFNETVNDGTVPSTSVFADCTVNGRAMRPTDFDLSSAVKGSKVTV
                     RNADGTSFTLTA"
     gene            complement(7458..8798)
                     /locus_tag="VF058_03555"
     CDS             complement(7458..8798)
                     /locus_tag="VF058_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207414.1"
                     /translation="MESRRAIVVGLVVVVAFTVFLVLLAEGAYDTWAAVLLAPLLVGV
                     SLPALSRQALREGDRTVFWILVTALILKLGGAIARHFVAFDVYEGGADAAAYDEWGVQ
                     ISDRFRDGDFTTGLESLSGTNFIRFLTGVVYTVIGPSTLGGFLVYSWLGYWGLYFFYR
                     AFVVAVPTGRSRSYAKVMFFLPSLLYWPSSVGKEAWMMFTLGIAAFGIARMLSGEVGA
                     GARWAIPGLWLASLVRPHVAGLMGIGLLVAFLVRRQDRSLREVAPIAKGVSLIVLALA
                     AAVLVVKADAFLKDQGIDTSGGVTSTIGQVTERTGQGDSEFSPSIVQSPQRAPIAVAT
                     VLFRPLPFEAHNAQALMASLESVFLLGFCLWRFRWGLAALRGFRDSPYLAFAMVYVGL
                     FIFAFSGIANFGLLARERVQLLPLFLALFFVPPRDRSIGGRRREEATQSLRPSA"
     gene            complement(8799..9827)
                     /locus_tag="VF058_03560"
     CDS             complement(8799..9827)
                     /locus_tag="VF058_03560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6207415.1"
                     /translation="MRATREYAIRAYEDADQPAVLELLGAALGPGPGGRRSPEFFGWK
                     HQENPFGRSLMLVAEEEGRIIGLRALMRWRFRAGDGTVSAARPVDTATHPDAQGRGVF
                     SRLTKEALSALKGSASLLFNTPNEKSLPGYLKMGWRVVGSIPISVKVRRPLRVAAGIR
                     SLRRADAAPSEGRPRILAETAEAVLSDDAAISSLLEDPGMASSGRLMTLRTLEYLRWR
                     FGAAPLLDYRAVRRDEDGAPAGMAIFRVRPRGRLWESTIAEILVRDGDPRLTAMLLRD
                     VAQAAPVDVLTCHVSGSPARGPLRRRGFLRAPLGMTFVTHPLDDGVRPDPTELSSWAL
                     SLGDLEVF"
     gene            complement(9824..10930)
                     /locus_tag="VF058_03565"
     CDS             complement(9824..10930)
                     /locus_tag="VF058_03565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6207416.1"
                     /translation="MKKKRILILIKGLGRGGAEQLLVSAAPYFDRDRFEYEVAYLLPW
                     KDAFVKELERLGLRVSCLEGGSGSVGWMWRLRRFIRERRVDLVHSHSPVAAVGARLVG
                     GPGLGRVYTEHNVWERYHPVTYWLNALSFPRNDQVFAVSDRVRLSIGYPRSLRFLPMP
                     KLETLYHGLDPAAVARWAGGDGVREELGIPDDAPIVGTVANFKHHKGYEDLLRAASLV
                     RRAIPDVRFVLVGRGPLEEEVRRQALDLGLSGTVVFAGYREDAPRLAQAFDVFALASV
                     HEGLSIALIEAMAMGTPAVVTNVGGIPEVVEQGKQGILVPPRDPGAMAHGITTVLRDA
                     PLRERLGAEARLRAATFDIRKAVTRMEQVYEELG"
     gene            complement(10927..12066)
                     /locus_tag="VF058_03570"
     CDS             complement(10927..12066)
                     /locus_tag="VF058_03570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6207417.1"
                     /translation="MKVLHVISTGRRRGGEIFAADLVRALAQAGFEQRVAVISSDGVA
                     AVPFDVPSHVFRLTGARVPGLRVRWGTVRALRALVREWRPDVVQAHGGQPHKYMVLAS
                     PRGTAPVVYRRIGSASYGNDRGLRRLVHARLMRRADAIVAVAEAVREETLRIFGLPED
                     LVVTIPNAVDPSRLVPSRSRGATRRSLGIPPGAPVVVSLGALSWEKDPLAHLAVADGI
                     RRVRDDVVHLLVGDGILRGSVVRTIGELGVERVRLLGVRADVPELLAAGDVLLLTSRT
                     EGMPAVVIEAGMLGLPVAGFDIAGVPEVVEHGATGLLAAPGDVEGLTRAVLDLLEDPV
                     ARREMGEAARERSGRFDIRSVAGQYVDLYRRLASDRSLSRSGEPA"
     gene            complement(12063..13226)
                     /locus_tag="VF058_03575"
     CDS             complement(12063..13226)
                     /locus_tag="VF058_03575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6207418.1"
                     /translation="MRFVINGLGTGGAERSLAELLPRLVAEGIEPSVVCLYRRDEGVQ
                     EDVRAAGFDVRFLSAGGRVGRVRELRGLLAEDRPDLVHTTIFEADLVGRLASASLRIP
                     LVTSLVNTSYDDVRLLDPNVRRWRLATARLVDGWTARRLTTWFHAITRTVAESAVQSL
                     GIPGDRITVIPRGRDAERLGAPSPERKERARRALALEPRDQVLLSVARQEFQKGQGTL
                     VSAMSRLPATAVLLIAGRDGNATATLRAAASSLGDRVRFLGHRSDVPELLAAADVFVF
                     PSIYEGLGGAVIEAMALGLPVVASDLPALHEVVEPGRSAVLVPAGNADRLAEAVGGLL
                     RDRPELGRLGRRGRELFLEKFTIEGVARRMSEMFRSVADVGRRPGREAGRGIG"
     gene            complement(13240..14130)
                     /locus_tag="VF058_03580"
     CDS             complement(13240..14130)
                     /locus_tag="VF058_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX6207419.1"
                     /translation="MRQQVLTKATTVARRAGSIVSPASRRWRRRLRSLTLDPDRYDAL
                     SAPGPDDFLICGSPRSGTSLVCAALFQPPRCVVAMEPWDGLRMTPRDLFASLRDEIEL
                     TGRLRRGRLDVDALYAEGAARWVREGEADVAVPTEGRFRLGVKWPAYWRYLDRLPDTR
                     FIVCLRHPVEVIRSFRSKGGRLGEGFDYDIPFNRSMNDELARATDSPEVRRVLMYEHI
                     NARLLPHLSRPNVLALRYERWFAEADAVLQELSAFLSVDLSEARIRIEQPRSGRDATD
                     EERELIRTHAPSAAALGYEL"
     gene            complement(14127..15047)
                     /locus_tag="VF058_03585"
     CDS             complement(14127..15047)
                     /locus_tag="VF058_03585"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014139183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6207420.1"
                     /translation="MKAHLRTTIDAVLRRSPAQPLFRLRNSDRLAVLAYHAIEDGGRF
                     EEHLGYLRRVAHPVSLSEVVDAARSRAMLPMHAVLVTFDDGDRSVLEVAAPLLERHGI
                     PGVAFLISGLIDSGRPTWWAEVEALVRSGGRAPALTASGPDEAVRALKRMPEERRRAT
                     IEDLRSSARLEAPPAGQLRAEELATLEAAGVEIGSHTATHEILPGCTDESVEAEIADA
                     HRALTESLGHAPRAFAYPNGDGDPRAENVLRRLGYEAAFLFDHRLARLPIEDPLSISR
                     VRIDAAASVDRLATVVTGLHPAVHHVRGQR"
     gene            complement(15056..16366)
                     /locus_tag="VF058_03590"
     CDS             complement(15056..16366)
                     /locus_tag="VF058_03590"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012992281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX6207421.1"
                     /translation="MRVTVVGTGHVGLVTCASLAALGHEVVGTDADGEKIAQLQRGLS
                     PFFEPGLDELVREGVASGRLNFTSEMAEAVPGAEVIFISVGTPPKVDGEANLIAVESV
                     AREVARHATVEGVLVEKSTVPAGTGARLRRAMRHESGAHLLEVASNPEFLSEGTAVRD
                     AMEPQRILVGADSERAFQAMRRLYAPLVERGTPLIETDVETAELAKYVCNAFLALKVS
                     FINAVARVCERTGADVVDVADVMGADPRIGRSFLNAGLGFGGSCFPKDVRAFQRLVGR
                     LGYEFPLLDEVVRVNEESLEATLEKVREALWNVEGKRVAVLGLSYKPGTDDVRFSPAM
                     VLVQRLLSEGATVVAYDPHALSNAKAEVPEIEVATDPYDAAEGAHCLVICTEWDEFHR
                     LDVERIARAMAHPLIVDGRNVLDPAVVGAAGLSYFPTGRPMVVR"
     gene            16524..17411
                     /locus_tag="VF058_03595"
     CDS             16524..17411
                     /locus_tag="VF058_03595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6207422.1"
                     /translation="MAGPQTIATVAVVVPTHNRPKLLVEAVESVLAQTFSDLQLVVVD
                     EASDPPAERVVGPTDPRLRVIRNDAPRGPAAARNQGVDAVSSRFVAFLDDDDRWLPTK
                     LEECLAGFRAHPQAGMVFHRMGSFRSPADRGRPIYRLVQEPVKRMLTRQPPHVDAVLI
                     RRAVHDAVRFDEAFPAAADLDYMLRVAMEADVLELNRLLAVHGPISERVSAIALERRI
                     AGRERFREKHSELFEDPEVRAFHELRLGHLHSRAGERMRAASAFVRALRIRPTWSLPW
                     KGLLGLPVPRGLLARLLER"
     gene            complement(17424..18515)
                     /locus_tag="VF058_03600"
     CDS             complement(17424..18515)
                     /locus_tag="VF058_03600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6207423.1"
                     /translation="MGETRSRPRPRPPSPLVETGASGGGLMSSGAEAQAPKEGPRPPT
                     AHATIVVTTCDRPEVATRAVASALRQSEPRVEVLVVDDGVRDRVPMDMGDDRVRVIRT
                     EGRIGVSRARNVGLAEAKAPWVSFLDDDDELLPTMVEEALAAARGSSLPPPVAVVAGM
                     EELDPEGFVLLTRIPVSMPRGRHYFLEDVAEKDRRGLAAYNTLFAPVDVVRGIGGFDE
                     QMVASMHLDLMLRLNAACSIQAVPRVGYRMYHHAEFRLSKQYRHRAESARRTHAKHRE
                     AFRQHPRMEGMYLARTGTFYLRAGEWLRPLGLTVRAFLRAPRRPRAMKQVLLAAAGPR
                     FFAWYEARKTRRAGGIWAPTSDDVSGRAR"
     gene            complement(18437..19417)
                     /locus_tag="VF058_03605"
     CDS             complement(18437..19417)
                     /locus_tag="VF058_03605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX6207424.1"
                     /translation="MSRSIVTTRSIGRDGREKGHPGRETPRIAVIIPTRDRAELLPRS
                     IESLLGQEFEDWEAVVVDDGSEEDIRSVVERYEEERIRYVRQAPSGAATARNTGVAVS
                     RAPYVTFLDSDDEAFPSWLGLLVEDFTRRDSDVVCCGAQFVFPPDHHRDPKAILPRDL
                     GRLFGNRRGLFTQSGTYALRRAVFDAIGGFTDSLPASEHTELSFRLLPAAEAHEWRID
                     VIDRPLIRYYLGRADGLRKNDRAVYEAATYVARTHRQLLKKDPRRLWNYLSVAGVRAA
                     RLGEYQAAARHFLEAVRVRPRSARGWARLALALVPGLRRRWWRRVHQEAG"
     gene            complement(19414..26328)
                     /locus_tag="VF058_03610"
     CDS             complement(19414..26328)
                     /locus_tag="VF058_03610"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX6207425.1"
                     /translation="MGGLLLGLALTMTSLAPASQAQSAYQLLVSVAPDRSGGSALHGS
                     TVEDSIYVFLAPESGVMQVRFWLDNPSMSGKPRTTERRAPWDFDGTVTSDGTAKPFDT
                     NGVGDGLHTISAQVDLSGGGTDVLHATFTVQNADAQLSFSPTSVDLAVDPGGTASAST
                     AVSSSEATSAAFQVTSSAPWLSATPASGNVPATLTVSVDATGLANGVYGGTVTASADG
                     HTPATFDVTLTVGEVGPADQIHLSWVDDPSTTMTVVWRTLAPDASSVVEYRSQGASAW
                     QQASGGTRTSGTNGTLREATITGLEPATTYEYRVNGGSEGWSPVHTTRTAPAPGPADF
                     SAIYFADTGLIGRGDGLATGTEQAIEEMAALDPTLFLPGGDYIYYDTDKRYGSLEDSI
                     DAWFTQNEPFATGAPMMPTYGNHEVLLGESYTAWSARFATPQGFDGGRFYSFDVGDVH
                     FVSILAVAETQGLSSQALDWIRQDIEAAKAAGQRWIVPYMHVPSFSDGKNHPSNLNLR
                     GQLGPLFEQLGVQVVLTSHDQSYERTYPLTDVPATNTPTSSSLSCYTMDDGVTYVKVS
                     PAGKLSNKNGDFSQFATNPPPHSTAFRDNTLHHFTRLAVSDEGALRVETYGFTGDGGS
                     PTVIDAFEYRDGTCPGELKTSPGVLNFTAVEGEVAGATVALTVSDGTAASFEAGTDAP
                     WLTAVPASGSTPASLSVQADASGLAPGTHQATLTATAPGYEPVDVPVTFKVTAVGGYR
                     LLVSGSADRSSPAHLEGRTLQGDAYVFTGPDEGVARVVFWLDDPSMSTKAFKTENNPP
                     FDLAGTAGSGAALPWDTTKMADGSHTITARLELSGGAVEVVHGTFVVSNLEPQLRFQP
                     GALSLSGEQGESPSAIVDLSVTDGSAATYALSTNRPWLTALPASGSTPRSITVTADTT
                     GLALGTHTGEVTAGAPGLDSLTLPVSVSVGLMGSSYRLLVSPSPDRSGFADLEGQTLQ
                     GNAYVFTGPEQGVSKVSFWLDNPAMTGTPTKVEKGAPYDFAGTAQDGRAFPWNTSSVS
                     DGSHTISALVELSGGGSEVVHATFTVANTAPQLRFQPTSLGLSGEQGDAPSATAALEV
                     TDGTQTAFTLSTDRPWLSAQPTSGTTPQAITVTGDTAGLAAGTYNGTVTATTAGFGSV
                     VLPVTLVVQAPGTGYRLLVSPTADRSTFADLEGQTLRGDAYVFTGPDQGVSKVSFWLD
                     NPQMTGTPTKVENAAPYDFAGTTSSGTAFPWDTTAVPDGQHTITALLELSGGGSQVVT
                     ATFLVANDVPSLAFTTDRLVFDVEEVGGTADPQTSGLITTDGVPAGFVVQDDADWLSV
                     SPISGTTPATLTLSLNTTGLPEGNHQAAVTATSPDHEPAVLLVTLAIGTGGCVPLACS
                     EIKVSLPYRLDWGVDHGKILAGNGVGTGFTWADFGGNGYLPEKLSVDTSQGVYRIQTT
                     SGGATGTENAQDNALGVGIAAPNQAFVMETEVVNPPAGTGKFEQAGLWFGNDQDNYVK
                     LIVLSTPGGTVIEYLIEVNGARPVNPKRTGALNLGASTATLILRANPFTRKIEGSYRV
                     DGGPEQVVATFSAPPEFFSFDAAGIDPRIGTRSFGGVFATHRTGSPLTYTFDSFSVVQ
                     DTSVANPTTPEGFSFDKKTFPVPNPTSMAWGPDGRLYVTELFGKIHAITLDTNKQVVS
                     DEVITTLGSRLTLGLTVDPASTASNVVLWVSHSSPSVNNGEVNSSTVTRLSGSGFTTR
                     QDVITGLPRAKANHAINSIHFGPDGRLYMAHGGNTGAGAPNLANTEFGDRSEQPLSAA
                     LLVADINAAGFEGQCATPLGQFGVPATCDVSVYASGLRNSYDFVHHSNGSIYAPDNGL
                     GVTGTYPPKPTPDCTGLADASQHNPGEQPDIILRLEQGKYYGHPNPYRNECVFKDGSK
                     QGVAPLPNYVPPIHTIGNNTSSNGTIEYTGDAFCGDLRGDLLIANYSVGDDLVRYKLA
                     PDGNSIAEVTRLATGFTDPLPVTQGPDGTIYVGELGAGQVAALVPQDTGCWSDRAPLP
                     AQLLDVGGTGLSGKFYVAAGKTSSGPRTNMYVYDPATDSWATGPNLPGPGVENPAMEA
                     LNGKLYVFGGSTDPFSGAVANAAVYDPGTNAWTTLTPMPTARGGANAKAINGLIYVVG
                     GMDANGASVAKVEVYNPSTNSWSAGPSMGTRRDNPGAAVLDGRLYVFGGRTREANGTT
                     VNGTLASVEMLDPATGTWVAKAPMPTGRRTMVVGTLNGRAQVMGGEASGIGDGTFPQN
                     EEYDPVTDTWRPLRPMKSPRHGAAAATISGVIYVAGGGTASGSSFSNVHEAFAFQR"
     gene            complement(26453..28282)
                     /locus_tag="VF058_03615"
     CDS             complement(26453..28282)
                     /locus_tag="VF058_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6207426.1"
                     /translation="MTDTIRKLIHLLRPEGLRQWAGVIALAVVASGVEAAAAILIFLL
                     IGLITAPGGRIELPVIGDISSRFSHVPQETLMSWVAGVVVVFFLLRGAITLLQLYVQS
                     RISQNVGARLAERLFTGYLRMPYPFHLERSSAELVRNTEETVRAITLDVLGPIVRLLS
                     GSVLAVAMLGVLVVTAPLATGFAVATLAPVVLLLLKVVQPRLRRWGGRRQHLAKANLS
                     VVQQALHGIRDVKLLDAVPFFSRVYLRQRRDMARVTYLGRVGMEFPSILIETVLVVFI
                     AVFFIVFLALEGGVGQLLAVLGMFAYVALRMQPALQRIVQSVTSLRFASRAVEHAYED
                     ILLTEARAEEEKEDQPSLEFRRELSLVDVSFWFPGRPEAALAEVNLTIAAGEAVGIVG
                     PTGGGKSTLLDIIAGLLRPVSGEVLVDGVPLEGRERAWQRELGVVSQAVFMIDGSIRQ
                     NIALGVADSEIDPSRLEAAVEVAQLSEFVHSLPDGLDTLVGERGVRVSGGQRQRVAIA
                     RALYRDAPVLILDEGTSALDNRTEAAFMEALHRSRAGRTIIMVAHRLSTVRGCDRIVL
                     IGDGRVVDAGTFEQVAARNDGLVQVGPEPSGPSQMQGRSTIPR"
     gene            complement(28279..29454)
                     /locus_tag="VF058_03620"
     CDS             complement(28279..29454)
                     /locus_tag="VF058_03620"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX6207427.1"
                     /translation="MADWRPVEPFLRELASFGVPGGPSDPLAVPDGGWPRALSGLVRE
                     RLTGIAAAALKEGALKLSEEEEAELLDRQQEAMCHALALERELLRLARAFADAGVELI
                     VLKGPAVAHTIYPDPSWRPFGDLDVLVRTADWRRACSLLPELGYRRRFPEPRPGFVER
                     FGHTAAHQGPAGLEVDLHRTLVGGPFGLWMDPDELFDSTETFELGGRTLRRLDDTALL
                     MHAFVHAALGHRPPLLLPLRDVAQAASSGVDRARLADWGRRWRLSVVYRHASRVLSES
                     VGVRLPEGLAPGTEERPSRRERRALDAYTTARRDRGGKSLGSLRAIRGLRAKAAYLIA
                     LLFPSREFLQSRSSEGGPTSYRRRWATPLRWMSQGRSRGHRSDDRDATAPQEMVGNP"
     gene            complement(29447..29734)
                     /locus_tag="VF058_03625"
     CDS             complement(29447..29734)
                     /locus_tag="VF058_03625"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX6207428.1"
                     /translation="MSDAPRLQHSPTVLARSVGERVILTAGDREGFEVLDGAGAMIWD
                     LLEEAVTPGEITATLAAWFEGPRDVIAQDVEGLLADLRQRGLIEEVDASRG"
     gene            complement(29731..>30856)
                     /locus_tag="VF058_03630"
     CDS             complement(29731..>30856)
                     /locus_tag="VF058_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX6207429.1"
                     /translation="AGVSEEMFRAAMATGRLSFSTEHAQLADHGVILICVPTPVRDNT
                     PDVSFIENACEKVAAELGPGCLVVLESTTYPGTTEELVRPILETSGFFADRDFLLAYS
                     PERIDPGNEEYGMRNIPRVVGGVTPEAAGLATLFYEQLVDKVFAVSSTRAAELAKLLE
                     NTFRHVNIALVNEMATLCHEMEIDVWEVIEATATKPFGFMPFSPGPGVGGHCIPLDPT
                     YLAWQVRRDAGHQFRILEQAEDVNAQMPRYVARRIGEALNEEGKPLRQSSILVLGVAY
                     KPDVGDVRESPAVNVMKHLVRTKAKLSFHDPYVESFTLNGEVLRRTELTLRSVTAADC
                     VALLTPHSAYDLEWIAEHARLIFDAQNAFGASVPTNVVRL"
BASE COUNT         4734 a        11234 c        10515 g         4373 t
ORIGIN      
        1 ctgaggcccg gatcgcctcg atggaggccc agctcgccgg cacccgcgcc gacctggagc
       61 agcaggcccg ggccgccgag acccgagcga ctcaggccga ggcggagctg gccgcggccc
      121 gcgccgagct cgccgcgaga ccggagaccc ccacccccgt ggacgcctcc gagcagatct
      181 cggcgatcat ccaggcctca gagcgggctg ccacccgcat catcgacgtg gcgggacagg
      241 ctcaccagga gcaggtcgag cagctcgacc ggatgcgggc tcaggtccgc gaggaggccg
      301 accgtatggt ctcctggcgg gagcagctcg gcccgatcgt ccactcgatc cggtccagca
      361 tcgaccgcgc tcgggccagg atcgacgaca tccccgaccg gatccgggag gcgctgaacc
      421 cgctcggcga ggcgatcgac tcggtcaacg accagctcac cagcctcgtg gaggaggtgg
      481 ccgagccggc gacgtccgag gacgagcgag gccccggggg cgcagaggac gatgatcgca
      541 gcgagccgga gcggccggac gattcctccg cacccatcag ggcgatccgc ctcgatgagc
      601 ccgaggccgc ccggaccggc gacgccacgg acgacccggg gacgcacccc ggctggcacc
      661 gccggtaggg atcaggcgac gcggggatcc ccgcggaggt ccctccacag gcctcggccc
      721 aagagcgctg cggccgcgca ggcgagggtg aagacggcaa cgccgaagga aagaggcgtc
      781 agcccgacca cgagagcccc tcctcccgcc aggatcgcca gggccccgaa ggcgatcgtg
      841 gtgcgaaggc ggtcgccgct gcgcgcagcg acgagatcgt aggagtgagc cctgtccccc
      901 ccggccatcg gacgccggaa gaccatcctc ctggccaccg tgaacaggag ctcgaaggcg
      961 aagacgccca ggcagagcgc cgcggccagg agccctcgcc atccgtcggt gctcagcgtc
     1021 gcagcgggga cggccagcag gacgcccacc gcgtaggctc ccccgttccc caggaagatc
     1081 ctcgccggag gcctgttcca gacgaggaag ccgatgagag cgcccgcggt ggcgagggcc
     1141 agcgccgcgc ccgggtcgcc ccctggccga gccagcagca gggccaggcc caacgccgcc
     1201 accgcgccca ggctccccgc gagcccatcc tgcccgtcga tgaggttcac cgcgttggtc
     1261 gttgccggca cgagcaggac cacaccgatg gcagcgaaga tgcccagcgg ctcgagccgg
     1321 agcccggcgc ccatcaggag caggcccgac cccacctgtg cgaggaaacg caccgccgcg
     1381 ggcagtggga acgcgtcgtc caccaccccc accacgaggg cgagggcgac cgcgcccagg
     1441 atccacccgg acgggagatc gcccaggatc cagagcgagg cggccatgga ggcgaccacc
     1501 gcgagcccgc ccgtgagggg gatgggtcgc cgatgaatct tgagctcacc tccgtcgggg
     1561 cgatccacca cacccgtgag gagcccgacc gcccgagcga gcggggtgag gacgatcccg
     1621 ccgatcagag cgacgaggaa ggtcacgtcg cctcgggacg ccgctcggcc ccgacgtccc
     1681 tcacccacac gttgctggcc gtccctgcgt gcacctcgag gcgggccgcg aggcggaacc
     1741 cgacccgctc gtagaaccgg ttcgcgtcct cgtcgtcggc tccgaccacg accttgacct
     1801 ccgtcgctcc tcgtcgcccc agcgccgtga gcacaccccc cgcgagcgcc ctgcccacac
     1861 ccttcgcccg cgcgcccggg gcaaccgcga tcgagaggag ttcggccggg ggaagggaga
     1921 ccgaggcctc cggatacgcg gccgtctctc gcatgcggcg acgcacctgt ccgctcgcca
     1981 gtcggggcgc cgcgctcagg aaggctcgaa ctcctcgccg cagggcgaac cggcggaaga
     2041 agcgcgggac cgacgggacc ccggtggcga agccgaggac ccggccctcc cgctccacca
     2101 cgagcgccac cccctgcggg tccgagatca gcgcctcgta gagctggcgg aggaagccgt
     2161 caccgagcgt ggggaggaag gcggtcggca tgctctcgcg atgcagcctg gccatcgctc
     2221 cggcatcccc cgaggtcgcg ggtctgagcc aggttcgccc atcgccgcgt gtcttgcgaa
     2281 ccagaaggcg agtggtctcc gcggcgaccc ggtccacgac cttccgctcg tcgaaccgct
     2341 cgatcgcccg ggccctcgcg tcccggccca tccgttcccg gagccccggg tcatcgatca
     2401 ggcgcacgat cgcctccgcg agacgccccg gggcgcgggg cggcacgagc agcccctcct
     2461 cgtcgtggcg cacgacctca cgacagccgc ggatgtcagt tagcaccagc ggccgcccca
     2521 tcgcggcggc ctcgatcgcc gaacgtggca tcccctctcg ccacgaaggc aggatgaaca
     2581 tgtccatcgc cgccaggagc tcgggcacgt cggtacgcca gccggcgaac gcgacgtgct
     2641 ccttggcccg ggcgatctcc tcctcggaga gcgagtcggc cttctcgggg tcccgctcgc
     2701 ccacgaccag gaaccgtgcc ttggggcggg ccagcctgac cacctccgcg gcccggaaga
     2761 gctcgcggta tcccttctcg gcaaccatcc tcccgatcgt cccgacgacg atctcgtcgg
     2821 gtctgatgcc gaggtcgcgg cggagctcat cgatctgtcc ccggtcgacc ttcgaggggt
     2881 cgaagcgcga gaggtccgtg ccgttgccga gcagcaccga ccggggaggg tcgaccagcc
     2941 gccggcgcct ggcccagagc aggtcctcct cgctctggta cagctccagg tcgctgaacc
     3001 acgccgccag ccactccagg ctcaggaccg ccgcgcgctt catcagccgg tcctggggcg
     3061 tggcgtagag gccgtgcacc gtgttcgcca cgcatgggac gcccgcggcc ctggcagcca
     3121 gcctccccag gatgcccggc ttcgggttgt gcgtgtgcac caggtcgaag ctctctcgcc
     3181 ggaggatccc caccagctca cggagcgctc ggaggtctcc cctcggatcc cacgagcggg
     3241 tcgcgtgcgg ccacggtacg tggcggatgc cctcccgttc caggtcggcc acccacggcc
     3301 ctggggcgct gatgcccacg acctcgtgcc cctcatccct gagcctgcgc agctgcccga
     3361 ggaggaggaa ccggagcgtc aagtccaccg tcgtgaggtg ggcgatgcgg ctcacgacca
     3421 cctccagggg ccccgccgca gggctcggta gatcagagcc tccccgtcga gcatgccccg
     3481 caccttggcc cggaaaaagc ggcgcccgtc gctcctcagg acgggggtcc tccccagggc
     3541 gtgcggatcg gtgtggccct tccggttcgt cctccacgca tggagcgcgg ccgtccggaa
     3601 cacgcgcctg gccgcgcggt cggcggcggg cgagcccgcc gcgaacgggt acgcgaagtg
     3661 gcggcactcg accccgagcc ggtcctcgat cagctccttc gagcgccgca tctcgtcctc
     3721 ggcctcccgc tcggagaccc ggaccaacgc ccggtgggag tgtgtgtggc ttcccaccgt
     3781 gacgagcccc gtcgcgaccg cctcttcgag ctgcgcccag ctcagcgagc cgttcccgcc
     3841 ttcgaccggg cccgtcgcga ggtagagcac ggccgggatg cggcaccgca ccagcacagg
     3901 cacgacgtgg gagtggaagt ccggtgtgcc gtcgtcgaac gtcaccacga cgccccctcg
     3961 cgagccgtcc gagaccgctt cgtccagccc cgtcggccga tcctcctcgg cgagcgtacg
     4021 catctggcgc tcgaagagct ccagcgggag gtcgatctcg ctcgatccca ccccgacccg
     4081 gtggtacagg agcacgacga ggtcgccgtc gcgccgcgga cgcagcacgc cggcgggcag
     4141 gaccgccgtc ttgatgccct ttcggaggag ctccatcgcg ttcaacgccc tccgctcctc
     4201 cggttccagg cggtcgtcga tccgatcagg gcggcggcgg cgatcgggat cgcagggaag
     4261 tgcagcacgt agtcgatcga ggcgtggatc ccgatcgccg ccgccgccgc cgccccgagc
     4321 gccgccaccg aatccggcgg gcccatccac agcctggcga agacccaggc gaaggcgagc
     4381 acgagcagga tcagacccgg gaagcccgtc tccgccccca cctcgaggaa ctcgttatgc
     4441 gcccaccgag cgtcggcgtc gtccaccgcg gtggggctga ggcgctggaa gctcccgatc
     4501 cccgcgccgg tgagcggctc ctccgccatg atgtccacgg catcacgcca caggacggcc
     4561 cgccgctccc ccaccgtcct ctcgatgatc ccgggctcgg cggagccgtc gctcggatac
     4621 gtggctccga ccacgatcgt cgccgcgagc gccgtcgcga acagccctgc catcagcccg
     4681 acggcgacgc gggggctcag ccaacgcgag actgcgagcg ccacgagggg cagcgccacg
     4741 acgagggcga acgccgccat ggaaccgctc ccgaagacca cggtgccgaa cccgatcgcg
     4801 gcggcccatc ccaccacgcg cagggccacc cagcggccgc tgacaccgat gatcactccc
     4861 gcaatcgcgg cctgggcgag gaacgccccc gtggcgttcg cgtacccgaa ggggcccccg
     4921 agtggaccgc tgcccacaag atcgtccggc gatcgcacga tcagcaccag cgctgcggca
     4981 cccagggccg ccgggatgat gaaaccgctc ctgatgcctg ctaccctgcc gaccaagacg
     5041 cccatgccgg aaaccgccac cagcccggag acgagggcgg gatcgtcgcc ggacacggtc
     5101 cccgccagga tcgtccaccc caccagggcc gcgaacagcg cgacgcccgc gacgtcgagg
     5161 acggctcccc ggagcttcgg cgtcgcgttc ccgccctctc cgacggatcg atcgatctca
     5221 gtcctcgtcg ccacaccgct cccatccgta ccgtcggtcg ccgtaggtcg cccctgcgct
     5281 cggcccccct cgatgtccga gggacgagcg ggggacgtca agagtcgccc cccgggcgac
     5341 cgataccaac agtctgatcc gcgtcgtcga tcgccacgag gtgggccgcg tgccaaccga
     5401 ggacctcagc cgaatccctg agtggatcac ggtccgtgag gcgtcgttcc tcaccgaccg
     5461 gtcgctcgat ggactcctgc gcctgatctg ggatggccga ctccgctcca cgtccgtcac
     5521 cgggagcccg gatcccagac tgctcctcgt cccgaccgcg gacctcgtcg acctcggtct
     5581 cctggaggcg gagagcccgg gcgtgggccc gatctcgatc gacgaacgac gacggcgagc
     5641 cgagcgcagg atcgcgtcgc cgtttgcgag ctgggggaag gtggccgcca tcctcgtcct
     5701 gttcgtcggt gcggcgctga ccgggccggt tcgatcctcc atcgtcgagc tcttcggggc
     5761 ccacgccgcg aagacctggc aggtcaagcg cacgccaccc tgccacagca actcgcacaa
     5821 gcggaagcag tgccagtcgc cgtcccccac cccctcgcct agcccggcgc cgaacccgag
     5881 ccccagcccc tctccggagc ccagtcccga gccgaccgag cccgttcctt cgccgacgct
     5941 cgagccgtcc cccgagccga gctcctcccg gagcgctccg ccggaggggg tgcatacggt
     6001 gtccggcttc gtcagcggcg gcttcacggt tccggccggc gaggtctggt cggtggacag
     6061 cctcgtggag accaacggga gcgtggtcgt gcacggcacc cttgtgatgc gaccgggcga
     6121 cacgcttcgg ttcgtgggcg tcgatgagtc ggcgttcgtg gggggcgaca cggcgccggt
     6181 cgcgaccgac gtcggcctgt gggtggaggg tcacggggtc ctcgacgctc gagggacgcc
     6241 gaaggtcgcc tggaaccgaa ccggcgccga cccgagctgg agcccgtccg acgagctcgt
     6301 tgtggccccg acatcgaccg gggactacgg gggagccggc ttcgcgtcct tcacgatggg
     6361 ctcggcggtc ccccaggtga acggtcaccc ggctgaggtc ctcaacctca cccgggacgt
     6421 gaacatcgag ggaaccagcg gcggcagggc ccacatccgc ttcaggtcct cccggcccca
     6481 ggtcatcgag tacgtcggca tccgccacat gggtccgcga cagcccggcg aggacggcta
     6541 cacggacgac gtcccggggc gaaaccccct ccacttccac atgatgggtg acgcgtcccg
     6601 ggggtcggtc atccggggtg tggtggtccg cgatgccgga gcgcacgcgt tcgtccccca
     6661 catgtcgcac ggcgtgacct tcgtggacac gatcgcctat gacgtcttcg aggccgcgta
     6721 ctggtggccg ccggtcgccg aggcgaacgc gacggggccc gagacgcacg acacgctcta
     6781 cctccgaagc gtcgcggcgc tggtcaagga cgatccgccg ttccgaggcc atcgcctctc
     6841 cggcttcttc ctcgcggccg gcgagggcaa cgccatccgg ggctccgtcg cggtgggtgt
     6901 ccagggcacc aagaacgcca gcgccttcca ctggcccgag cggagcacgg gttcgacgac
     6961 ctccggcgtg tgggacttcg acgacaacgt cgcccacaac aacaaggtcg acggagtctt
     7021 cgtgtggcag aacaacacac gtgcccacgt ggtcgaccac ttcatcacgc agggcagcgg
     7081 gcaggccggg atcgagcacg gcgcctacgg gaaccgctac gtgtacagtg acttcacgct
     7141 ccgtgaggac cgcctcgggg tgctgctcca cgctgccggg gcgaccttcc gggacgctca
     7201 gatcagcgct cccgtgggcg tcgtcttcgg gaagcaccgc ttcgacagcc ccgagacgct
     7261 gttcagtcgc gtcgcgttcc aggtctcggg gaaggcggtg gtcttcaacg agacggtgaa
     7321 cgacggcact gtgccctcga cctcggtctt cgccgactgc acggtgaacg gccgggcgat
     7381 gcgccccacg gacttcgacc tctcctccgc ggtcaagggg tcgaaggtca ccgtgcgcaa
     7441 cgccgatggg acgagcttca cgctgacggc ctgaggctct gggtcgcctc ctccctcctc
     7501 ctgcccccga tcgagcgatc gcggggcggc acgaagaaga gggccaggaa caaggggagc
     7561 agctgcaccc tctcccgcgc caggagaccg aagttggcga tgcccgagaa ggcgaagatg
     7621 aacagcccca cgtacaccat ggcgaaggcc aggtacggcg agtcccgaaa gccccgaaga
     7681 gcggcaaggc cccatcggaa cctccacagg cagaagccca agaggaacac ggattcgagg
     7741 ctggccatga gggcctgggc gttgtgggcc tcgaagggca acggacggaa caggacggtg
     7801 gccacggcga tcggcgcgcg ctgaggcgac tgcacgatcg atggggagaa ctccgagtcc
     7861 ccttgaccgg ttcgctcggt cacctgcccg atcgtcgagg tcacgccgcc gctcgtatcg
     7921 atcccctgat ccttcaggaa ggcatcggcc ttcaccacga ggaccgccgc cgcgagggcc
     7981 aggacgatga gcgagacgcc cttcgcgatg ggagccacct cgcggaggga tcgatcctgg
     8041 cgtcggacga ggaaggcgac gagcaggccg atgcccatca gcccggcgac atggggcctt
     8101 accagagagg ccagccacag ccccgggatc gcccatcgcg cgccggcccc cacctcgccg
     8161 ctcagcatcc gagcgatccc gaacgccgcg atcccgagcg tgaacatcat ccaggcttcc
     8221 ttcccgacgc tcgagggcca gtaaaggaga gaggggagga agaacatgac cttggcgtac
     8281 gatctcgagc ggccggtcgg cacggcgacc acgaaggccc ggtagaagaa gtagagcccc
     8341 cagtacccga gccacgagta aacgaggaag cccccgagcg tgctcggtcc gatcaccgtg
     8401 tagaccacgc cagtgaggaa ccggatgaag ttggtaccgc tcagcgactc cagccccgtc
     8461 gtgaaatcgc cgtcgcggaa gcgatccgag atctgcaccc cccattcgtc gtaggcggcg
     8521 gcatccgctc cgccctcgta gacgtcgaac gccacgaagt ggcgcgcgat cgccccaccg
     8581 agcttcagga tgagggcggt gaccaggatc cagaagacgg tccggtcccc ctccctgagg
     8641 gcctgacggg agagggccgg gagggacacc ccgacgagca ggggcgcgag gagcaccgcg
     8701 gcccacgtgt cgtaggcgcc ctccgcgagg agcacgagga agacggtgaa cgcgaccacc
     8761 accacgaggc cgacgacgat cgctctgcgg ctctccactc agaacacctc gaggtcgccg
     8821 agcgacaggg cccacgagct cagctcggtg gggtccggcc gcacgccgtc gtccaggggg
     8881 tgcgtcacga aggtcatgcc caggggcgcg cgaaggaacc ccctccgcct gagggggccg
     8941 cgagcgggag agcccgagac gtggcaagtg agcacgtcca cgggggcggc ttgggccaca
     9001 tctcggagca gcatcgccgt gaggcgagga tccccgtccc gaaccaggat ctcggcgatc
     9061 gtcgactccc acaggcggcc cctgggcctc acgcggaaga tcgccatgcc cgccggcgcc
     9121 ccgtcctcgt cccgacgaac cgcccgatag tccagcagcg gcgccgcccc gaaccgccat
     9181 cgcaggtact cgagcgttcg gagggtcatc agcctgccgc tcgaggccat gccggggtcc
     9241 tcgaggaggg acgagatcgc cgcgtcgtcc gagaggacgg cctcggccgt ctcggcgagg
     9301 atccgcggcc gtccctcgga cggcgcggca tcggcccgcc gcagcgaccg gatcccggcc
     9361 gcgacgcgca gcggccgacg gaccttcacc gagatcggga tcgagccgac gacccgccat
     9421 cccatcttca ggtatccggg caggctcttc tcgttggggg tgttgaagag caggctcgcg
     9481 gagcccttga gagccgacag cgcctccttc gtgagccgag agaagacccc gcgcccctgg
     9541 gcgtcggggt gcgtcgcagt gtccacgggc ctcgccgcgg agaccgttcc gtcaccggcc
     9601 cggaaccgcc accgcatgag ggcccgcagc ccgatgatcc gaccttcctc ctcggcgacg
     9661 agcatcaggg agcggccgaa gggattctcc tggtgcttcc acccgaagaa ctcgggcgac
     9721 cggcgcccac cgggacccgg tcccagagct gcccccagga gctcgaggac ggccggctgg
     9781 tccgcgtcct cgtaggccct gatcgcgtac tcgcgggtgg ccctcacccg agctcctcgt
     9841 agacctgctc catgcgcgtg accgccttcc ggatgtcgaa ggtggccgct cgcaggcggg
     9901 cctcggcccc gagccgctcc ctgagcggcg catcccggag gaccgtcgtg atgccgtggg
     9961 ccatcgcccc cggatccctg gggggcacga ggatcccctg cttgccctgc tccacaacct
    10021 cggggatgcc tccaacgttc gtgacgacgg ccggcgtccc catggccatt gcctcgatga
    10081 gggcgatcga cagtccctcg tgaaccgacg cgagggcgaa cacgtcgaac gcctgggcca
    10141 ggcgcggggc gtcctcccgg tagccggcga acacgaccgt cccgctcagc ccgagatcga
    10201 gggcctgtcg ccggacctcc tcctcgagcg gtccccggcc gacgagcacg aaccggacgt
    10261 ccgggatcgc tcgccggacc agcgaagccg cacgcagcag gtcctcgtag cccttgtggt
    10321 gcttgaagtt tgccaccgtc cccacgatcg gggcgtcgtc ggggatgccc agctcctccc
    10381 gcaccccgtc ccctccggcc cagcgagcca cggccgccgg atccaggccg tggtacaggg
    10441 tctccaactt tggcatcggg aggaagcgga gcgaacgtgg gtacccgatc gagaggcgca
    10501 cccgatcgga gacggcgaac acctgatcgt tccgggggaa cgacagggcg ttcagccagt
    10561 aggtcacagg atggtatcgc tcccagacgt tgtgctcggt gtagacgcgg ccgagcccgg
    10621 gccctccgac cagccgcgcc ccgaccgccg ccaccggcga gtgcgagtgc acgagatcga
    10681 cgcgtcgctc ccggatgaag cgccgcaggc gccacatcca gccgacgctg cctgagccgc
    10741 cctccagaca gctcaccctg aggcccagcc tctccagctc cttgacgaag gcgtccttcc
    10801 acggcaagag gtaggccacc tcgtactcga atcggtcgcg atcgaagtag ggcgccgcgc
    10861 tgaccagcag ttgctcggcg ccgcctcgac caaggccctt gatgaggatg aggatccgct
    10921 tcttcttcat gcaggctctc cgcttctcga gagggaacga tcgctggcga ggcgccggta
    10981 gagatccacg tactgcccgg ccacgctgcg gatgtcgaag cggccggagc gctccctggc
    11041 cgcctccccc atctcgcgtc gggccaccgg gtcctccaac aggtcgagca cggcgcgcgt
    11101 tagcccctcg acgtccccgg gggcggccag caggcctgtc gctccgtgct ccacgacctc
    11161 gggcacgccg gcgatgtcga acccggcgac cggcaggccc agcatcccgg cctcgatcac
    11221 gacggccggc atcccctccg tcctgctcgt caggagaagc acgtcgccgg ccgcgaggag
    11281 ctcggggacg tcagcccgga ccccgagcag ccgcactcgc tcgaccccca gctccccgat
    11341 cgtccggacc accgatcctc ggaggatccc gtcccccacg aggagatgga ccacatcgtc
    11401 ccggacccgt cggatgccgt ccgcaaccgc caggtgggcc agcgggtcct tctcccaact
    11461 gagcgcgccc agcgagacca cgacgggggc cccgggcggg atccccagcg agcgtcgggt
    11521 cgccccccgg ctcctcgagg ggacgaggcg gctcggatcc accgcgttgg ggatcgtcac
    11581 gacgaggtcc tcgggtagcc cgaagatgcg cagggtctcc tcgcgaacag cctcggccac
    11641 cgccacgatc gcgtcggcgc gccgcatcag gcgtgcgtgc acaaggcgac ggagcccgcg
    11701 gtcgttgccg tacgaggccg acccgatccg ccgatacacc accggggcgg tcccccgagg
    11761 gctcgccagg accatgtact tgtggggctg gcccccgtgg gcctggacga cgtcaggccg
    11821 ccactcgcgg acgagcgcac gcagcgcccg gacggtcccc caccgcaccc gcagtcccgg
    11881 gacgcgggcc ccggtgagtc tgaagacatg gctcggcacg tcgaacggta ccgcggccac
    11941 gccgtccgag ctgatcacgg cgacccgctg ctcgaacccg gcctgggcga gggcccgcac
    12001 gagatcggca gcgaagatct cgccgccgcg gcggcggccg gtggagatga cgtgcaggac
    12061 cttcatccga tgccccgtcc cgcttcgcgg ccgggtcggc gccccacgtc ggccaccgac
    12121 cggaacatct ccgacatcct cctcgccacc ccctcgatcg tgaacttctc caggaagagc
    12181 tccctccccc gccgcccgag acggccgagc tcggggcgat cccgcaggag gccgcccacg
    12241 gcctccgcca gccggtcggc gttcccggcc ggcacgagca cggccgagcg gccgggctcg
    12301 accacctcgt ggagggccgg caggtcgctc gccacgaccg ggaggccgag cgccatggcc
    12361 tcgatcaccg ccccgcccag cccctcgtag atcgacggga agacgaagac atccgccgcc
    12421 gcgagcagct cggggacgtc agatcggtgc cccaggaacc gaacgcgatc cccgagcgag
    12481 ctggccgcgg cccgcagcgt cgctgtcgcg ttgccgtcgc ggcccgcaat cagcaggacg
    12541 gccgtcgccg ggagccggga catcgcagag acgagcgtgc cctgtccttt ctggaattcc
    12601 tgtcgtgcca cgctgagcag cacctgatcg cggggctcga gggcgagcgc gcgccgggcc
    12661 cgctccttgc gctcggggct gggcgctccg agccgttcgg cgtcgcgccc ccgggggatc
    12721 accgtgatcc gatctccggg gatcccgagg gattggaccg cggactcggc caccgtccgc
    12781 gtgatggcgt ggaaccaggt cgtgaggcgc cgcgcggtcc agccgtcgac caggcgggcc
    12841 gtcgccaggc gccagcgccg aacgttcggg tccagcaggc ggacgtcgtc gtacgaggtg
    12901 ttgacgaggc tcgtgaccag cgggatgcgg aggctcgcgg aggccagccg ccccacaagg
    12961 tcggcctcga agatcgtcgt gtggacgaga tccggccgat cttctgccag cagcccacgg
    13021 agctcccgga cccgtcccac ccgaccgccc gccgagagga accgcacgtc gaagcctgcc
    13081 gcgcggacgt cctcctggac cccctcgtcg cgccggtaaa ggcagacgac cgagggctcg
    13141 atcccctcgg ccaccagccg ggggagcaac tcggccaggg accgctccgc cccgcccgtg
    13201 cccaggccgt tgatgacgaa ccgcaccctc accggctgct cacagctcgt acccgagggc
    13261 cgcggcgctc ggtgcgtggg tccggatcag ctcgcgctcc tcgtcggtcg cgtcgcgccc
    13321 cgaccgcggc tgctcgatcc ggatcctcgc ctcggacaga tcaacgctca ggaacgcgct
    13381 caactcttga aggaccgcgt ccgcctccgc gaaccagcgc tcgtagcgga gggccaggac
    13441 gttgggccga gagaggtgcg ggaggagccg ggcgttgatg tgctcgtaca tgaggacgcg
    13501 ccggacttct ggggagtcgg tggcgcgggc cagctcgtcg ttcatcgacc ggttgaaggg
    13561 gatgtcgtag tcgaagccct cgcccagccg gccccccttg cttcggaagg atcggatcac
    13621 ctcgaccggg tgcctcaggc agacaatgaa cctcgtgtca ggcagccgat ccaggtagcg
    13681 ccagtacgcg ggccacttca cgccgaggcg gaaccggccc tccgtcggca cggcgacgtc
    13741 ggcctcgcct tcgcggaccc accgggcggc gccctccgcg tacagggcgt cgacgtcgag
    13801 gcgccctcgg cgcaggcggc cggtcagctc gatctcgtcg cgaagcgacg caaagagatc
    13861 cctgggcgtc atgcggaggc cgtcccacgg ctccatggcc acgacgcatc ggggcggctg
    13921 gaagagggcg gcgcacacga ggctcgtccc gctccggggc gacccgcaga tcaggaagtc
    13981 gtccggcccc ggtgcggaca gggcatcgta gcgatccggg tcgagcgtca gcgaccggag
    14041 gcgccgacgc caccggcggg aggccggaga gacgatgctg cccgcgcgac gcgcgacggt
    14101 cgtggccttg gtcaggacct gttgtctcat cgttgccccc tcacgtgatg gacggccggg
    14161 tggaggcccg tcacgacggt cgccaaccgg tccacggagg cggccgcgtc gatccgaacc
    14221 ctggagatgc tcaaggggtc ctcgatcggg aggcgggcca gccgatgatc gaagaggaag
    14281 gccgcctcgt agccgaggcg ccgcaggacg ttctcggccc gagggtcccc gtccccgttg
    14341 gggtacgcga aggccctcgg ggcgtgtccc agcgactcgg tgagcgccct atgagcgtcg
    14401 gcgatctcgg cctcgacgct ctcgtcggtg cagccgggga ggatctcgtg ggtcgcggtg
    14461 tggctcccga tctcgacccc cgcggcctcg agcgtcgcga gctcctcggc ccggagctgc
    14521 ccggcgggcg gggcctcgag ccgtgccgac gagcgcaggt cctcgatcgt cgcccgacgt
    14581 cgctcctccg gcatccgctt cagggcgcgg actgcctcgt cgggcccgga cgccgtgagc
    14641 gcgggtgccc ggccgcctga ccgaaccagg gcctcgacct cggcccacca tgtgggccgc
    14701 ccgctgtcga tcaggcccga gatgaggaag gccacgcccg ggatgccgtg tcgctcgagg
    14761 agcggggccg ccacctcgag gacgctgcga tccccgtcgt cgaacgtgac taggaccgcg
    14821 tgcatcggaa gcatcgcccg gctccgcgcg gcgtcgacga cttccgaaag gctcaccggg
    14881 tgcgccaccc gccgcaggta cccgaggtgc tcttcgaagc ggccgccgtc ctcgatcgcg
    14941 tgataggcga gcacggccag ccggtccgag ttccgcaggc ggaacagggg ctgggccggg
    15001 ctccgccgga gtaccgcgtc gatcgtcgtt cgcaggtgcg ccttcatcgg tctgcctacc
    15061 ggaccaccat cggacggccc gtcgggaagt agctcagccc ggccgccccc accaccgcgg
    15121 ggtcgagcac gttccgcccg tccacgatca gcgggtgggc catggcccgg gcgattctct
    15181 cgacgtcgag ccggtggaac tcgtcccact cggtgcagat cacgaggcag tgggcgccct
    15241 ccgccgcgtc gtaggggtcg gtggcgacct cgatctcggg cacctcggcc ttcgcgttgg
    15301 agagcgcatg cgggtcgtac gccacgaccg tggccccctc cgacagcagc cgttggacca
    15361 ggaccatcgc gggcgagaac cgcacgtcgt ccgtgcccgg cttgtacgag agccccagca
    15421 cggcgacgcg cttgccctcg acgttccaga gggcctcccg caccttctcc agggtcgcct
    15481 cgagcgactc ctcgttgacg cgaacgacct cgtcgagcag ggggaactcg taccccagcc
    15541 ggccaacgag gcgctggaac gctcgcacgt ccttcgggaa gcacgagcct ccgaagccca
    15601 ggccggcgtt gaggaacgat ctgccgatcc gcggatcggc gcccatcacg tcggccacgt
    15661 ccacgacgtc ggcgccggtg cgctcgcaga ctcgggccac cgcgttgatg aacgacacct
    15721 tcaacgctag gaaggcgttg cagacgtact tggccagctc ggcggtctcg acgtcggtct
    15781 cgatgagcgg agtcccgcgc tcgacgagcg gcgcgtagag gcgtcgcatc gcctggaagg
    15841 cgcgctcgga gtccgctccc acgaggatgc gctgcggctc catcgcgtcg cggacggccg
    15901 tcccctcgga caggaactcc ggattggacg cgacctcgag gagatgcgcg cccgactcgt
    15961 gccgcatcgc gcgccgcagg cgagctccgg tgccggccgg cacggtggac ttctccacga
    16021 ggacaccttc gaccgtggcg tgacgcgcga cctccctggc cacgctctcc accgcgatca
    16081 ggttcgcctc cccgtcgacc ttgggcggcg tccccaccga gatgaagatg acctcggcgc
    16141 ccggcacggc ctcggccatc tcggaggtga agttcagacg gcccgacgcc acgccctcgc
    16201 ggacgagctc gtcgagcccc ggctcgaaga acggcgagag gccgcgctgg agctgcgcga
    16261 tcttctcccc gtccgcgtcc gtcccgacca cctcgtggcc cagcgcggcc agggacgcgc
    16321 aggtcaccaa ccccacgtgc cccgttccca ccacggtcac tcgcatgttc agcctccttg
    16381 atcgctctga tagacggctc ccccgacggt tcgcgggggc gctcgatccc gcacgggcgg
    16441 acggtgttcg cgctagattc gggggccgcg gcaccggaag gagttcaccc gatggcccga
    16501 gtcccgaacc ggtcggatcc ctgatggcag gccctcagac catcgccacc gtcgccgtcg
    16561 tggttccgac ccacaaccga cccaagctac tggtcgaggc ggtggagagc gtcctggccc
    16621 agacgttctc cgatctccag ctcgtcgtgg tggacgaggc ctccgatcct ccggccgagc
    16681 gcgtggtcgg accgaccgat ccccgcctcc gtgtgatccg gaacgacgca cctcgcggcc
    16741 cggccgcagc gaggaaccag ggcgtggacg ccgtgtcctc aaggttcgtc gccttcctcg
    16801 acgacgacga ccgctggctc ccgaccaagc tcgaggagtg cctggcgggc ttccgggctc
    16861 atccccaggc ggggatggtg ttccaccgca tggggtcgtt ccgctcacct gcggaccgcg
    16921 gccgccccat ataccggctg gtccaggagc ccgtcaagcg gatgctgacg agacagcccc
    16981 cccacgtcga cgccgtcctg attcgccgcg ccgtccacga cgccgtcagg ttcgacgagg
    17041 cgttcccggc ggccgcggac ctcgactaca tgctccgcgt ggcgatggaa gcagacgtgc
    17101 tcgagctcaa ccgactgctc gcggtccacg gacccatctc ggagcgggtc tccgccatcg
    17161 ccctcgagag gaggatcgct gggcgagagc gattccggga gaagcactcc gagctcttcg
    17221 aggatccgga ggtccgcgcc ttccacgagc tccgtctcgg tcatctccac agcagggccg
    17281 gggagcggat gagggccgcg tccgccttcg tcagagccct gcgcatccgg ccgacgtgga
    17341 gcctgccctg gaaggggctg ctcgggctcc cggtcccacg cggtctgctc gctcgcctgc
    17401 tcgagagata agcgcccgga ccgtcagcgg gcccggccgc tcacgtcgtc cgaggtgggg
    17461 gcccagatcc cccccgcgcg ccgcgtcttc ctcgcctcgt accaggcgaa gaacctggga
    17521 cccgcggcgg cgaggagcac ctgcttcatc gccctgggtc gacggggcgc ccgcaggaac
    17581 gcacggacgg tgaggccgag cgggcggagc cactcgccgg cccgcaggta gaacgtgccc
    17641 gtgcgtgcga ggtacatccc ctccatgcgg ggatgctgcc tgaacgcctc ccgatgcttg
    17701 gcgtgggtcc tcctggccga ctcggcccgg tgccggtact gcttggagag acggaactcg
    17761 gcgtggtggt acatccggta tccgacccgg gggacggcct ggatcgagca cgcggcgttg
    17821 aggcggagca tgagatccag atgcatggac gcaaccatct gctcgtcgaa gcccccgatc
    17881 ccccgcacca cgtctacggg cgcgaagaga gtgttgtaag cggcgagccc gcgccgatcc
    17941 ttctccgcca cgtcctcgag gaagtagtga cggcctcggg gcatgctcac cgggatgcgg
    18001 gtcagcagga cgaagccctc gggatccagc tcctccatgc cggccacgac ggcgacgggc
    18061 gggggaagcg aggaaccccg ggctgccgcg agcgcctcct ccaccatggt cggcaggagc
    18121 tcatcgtcgt cgtccaggaa ggagacccac ggggccttcg cctccgccag gccgacgttg
    18181 cgggccctcg acacccctat gcgcccctcg gtgcggatca cccggacgcg gtcgtctccc
    18241 atgtccatcg gaacacgatc tcgcacgccg tcgtcgacca cgaggacctc gacccgcggc
    18301 tccgactggc gcagcgcgct ggccacggcc cgtgtcgcta cctccggccg atcgcaggtg
    18361 gtaaccacga tcgtcgcatg cgccgtgggc gggcgtgggc cttccttggg cgcctgggcc
    18421 tccgctcccg aggacatcag cccgcctcct gatgcacccg tctccaccag cggcgacgga
    18481 ggcctgggac gagggcgaga gcgagtctcg cccatccccg cgccgatcgc gggcggacgc
    18541 ggaccgcctc gaggaagtgg cgggccgccg cctggtattc cccgagcctg gccgctcgca
    18601 cccccgccac cgacaggtag ttccacagcc gtcgaggatc cttctttagg agctgccgat
    18661 gggtcctcgc gacatacgtc gccgcctcgt agaccgctcg atcgttcttc cgaagtccgt
    18721 ccgcacgccc caggtagtag cggatcagag gccggtcgat gacgtcgatg cgccactcgt
    18781 gggcctcggc cgcggggagc aggcggaacg agagctccgt atgctccgac gcgggaaggg
    18841 agtcggtgaa tcctccgatc gcgtcgaaga cggcgcgcct cagcgcgtag gttcccgact
    18901 gggtgaagag cccccgacgg ttgccgaaga gacgccccag gtctctcggg aggatggcct
    18961 tcggatcgcg atggtgatcc ggcgggaaca cgaactgcgc cccgcaacac accacgtcgc
    19021 tgtccctgcg cgtgaagtcc tcgacgagca agcccagcca cgaggggaag gcctcgtcgt
    19081 cgctgtccaa gaaggtcacg tatggagctc ggctgacggc tacccccgtg ttccgagcgg
    19141 tcgcggcccc cgacggagcc tgcctcacgt accggatccg ttcttcctcg tatcgctcga
    19201 cgacggatct gatgtcctcc tcagagccgt cgtcgacgac gaccgcctcc caatcctcga
    19261 attcctgtcc gaggaggctc tcgatggacc tggggagcaa ctccgcacga tcccgggtgg
    19321 ggatgatcac cgcgatgcgc ggtgtctcgc gcccggggtg acccttctct cgcccatcac
    19381 ggccgatgct cctcgtagtc acgatcgatc tgctcaccgt tggaaggcga acgcctcgtg
    19441 cacgttcgag aacgaggatc ccgaggccgt cccgccgccc gcgacgtaga tcacgccgct
    19501 gatggtcgca gcggccgcgc cgtgccgcgg tgacttcatc gggcggagcg gccgccacgt
    19561 gtctgtcacc gggtcgtact cctcgttctg cgggaacgtt ccgtccccga tgccggacgc
    19621 ctcgccgccc atcacctgcg cccgcccgtt cagcgtcccc acgaccatcg tacggcgccc
    19681 ggtcggcatc ggagccttcg caacccacgt gcccgtggcc gggtcgagca tctccacaga
    19741 ggccagcgtg ccgttgacgg tcgttccgtt ggcctcacgc gttcggccgc cgaacacgta
    19801 gagccgcccg tcgagcacgg ccgctccggg gttgtcgcgc cgggtcccca tcgagggacc
    19861 ggccgaccag ctgttcgtcg aggggttgta gacctcgacc ttggccacgg acgccccgtt
    19921 cgcgtccatg ccgcccacca cgtagatcag cccgttgatc gccttcgcgt tcgcaccgcc
    19981 cctcgcggtg ggcatagggg tcagcgtcgt ccacgcgttc gtccccgggt cgtagaccgc
    20041 cgcgttcgca acggccccag agaatggatc cgtggatccg ccgaacacgt acagcttgcc
    20101 gttcagagcc tccatcgcgg ggttctcgac gcccggtccg ggcaagttgg ggccggtcgc
    20161 ccacgagtcg gtcgccgggt cgtagacgta catgttcgtc ctcggcccgc tgctcgtttt
    20221 cccggccgcc acgtagaact tgccgctcag ccccgtgcct ccgacgtcga ggagctgcgc
    20281 cggcagcgga gcgcgatcgc tccagcaccc ggtgtcctgc ggcacgaggg cggccacctg
    20341 ccccgcgccc agctcgccga cgtagatggt gccgtcgggc ccctgggtga ccgggagcgg
    20401 atccgtgaac cccgtagcga ggcgcgtcac ttccgcgatg gagtttccgt ccggcgccag
    20461 cttgtaccgg acgagatcgt cccccaccga gtagttcgcg atcaggaggt ccccccggag
    20521 gtcgccgcag aacgcgtcgc ccgtgtactc gatcgtcccg ttggacgacg tgttgttgcc
    20581 gatcgtgtgg atcgggggga cgtagttggg cagcggcgcg acgccctgct tgctcccgtc
    20641 cttgaacacg cactcgttcc ggtaggggtt ggggtgaccg tagtacttcc cctgctccag
    20701 gcgcaggatg atatccggct gctcccccgg gttgtgctgg ctcgcgtctg cgaggcccgt
    20761 gcagtccggc gtcggcttcg gcgggtaggt gcccgtcacc ccaagcccgt tgtcgggcgc
    20821 gtagatgctg ccgttcgagt gatgcacgaa gtcgtaggag ttgcgaagcc cgctcgcgta
    20881 cacgctcacg tcgcaggtgg ccgggacccc gaactggccc agcggcgtgg cgcactgtcc
    20941 ctcgaagccg gccgcattga tgtcggccac gagcagcgca gcggagagcg gctgctcgga
    21001 acgatcaccg aactcggtgt tggcgaggtt cggtgcgccc gctcccgtgt tgcccccgtg
    21061 cgccatgtag aggcggccgt cggggccgaa gtggatcgag ttgatggcgt ggttcgcctt
    21121 cgccctcggc aggcccgtga tcacgtcctg tctcgtggtg aacccggagc cggagagccg
    21181 cgtgacggtg ctggagttca cctcgccgtt gttgacggag ggactcgagt gcgaaaccca
    21241 gagcaccacg ttgctcgccg tcgaggccgg atccaccgtg agccccaggg tcagcctgct
    21301 cccgagggtc gtgatgacct cgtcgctgac gacctgcttg ttcgtgtcca gggtgatcgc
    21361 gtggatcttc ccgaacagct ccgtcacgta gagcctgccg tcgggccccc acgccatgga
    21421 ggtcgggttg ggaaccggga aggtcttctt gtcgaaggag aagccttcgg gggtcgttgg
    21481 gttcgccacg gacgtgtcct ggaccacgga gaagctgtcg aacgtgtacg tgagcggcga
    21541 ccccgtgcga tgcgtggcga agaccccacc gaagctccgg gtcccgatcc gtgggtcgat
    21601 gcctgcggcg tcgaagctga agaactccgg tggtgcagag aaggtggcga cgacctgctc
    21661 cggccccccg tcgacacggt acgagccctc gatcttcctg gtgaacgggt tggcccggag
    21721 gatgagcgtc gcggtcgacg cgcccaggtt cagagcaccg gtccgcttcg ggttgaccgg
    21781 acgggcgccg ttcacctcga tcagatactc gatcactgtc ccgcccgggg tggagaggac
    21841 gatgagcttc acgtagttgt cctggtcgtt cccgaaccag agacccgcct gctcgaactt
    21901 ccccgtgccc gcgggagggt tgaccacctc ggtctccatg acgaacgcct ggttgggcgc
    21961 ggcgatcccc actcccaggg cgttgtcctg agcgttctcc gtcccggtag cgcctcccga
    22021 cgtggtttgg atccgataga cgccctggga ggtgtccacg ctgagcttct cgggcaggta
    22081 gccgttcccg ccgaaatccg cccacgtgaa gccggtcccc acgccgttgc cggcgaggat
    22141 cttcccgtgg tcgacacccc agtcaagccg gtacgggagc gagaccttga tctccgagca
    22201 cgccagcggg acgcacccac cggtcccgat ggccagggtc accagcagga cggccggctc
    22261 gtggtccggg gacgtggccg tcaccgcggc ctggtggttc ccttcgggga ggccggtcgt
    22321 gttcaagctg agcgtcaggg tggcgggcgt cgttcccgag atcggactca ccgacagcca
    22381 gtcggcgtcg tcctgcacca cgaagccggc ggggacgccg tcggtcgtga tcaggccgga
    22441 ggtctggggg tcggccgtcc ctcccacctc ctccacgtcg aagacgagtc tgtccgtggt
    22501 gaaggccagg gatgggacgt cgttcgcgac gaggaaggtc gccgtcacga cctggctccc
    22561 gccccccgag agctcgagca gcgccgtgat cgtgtgctgg ccgtcgggga cggccgtcgt
    22621 gtcccagggg aaagcggttc ctgagctcgt cgtcccggcg aagtcgtacg gcgcggcgtt
    22681 ctccaccttc gtgggcgtgc cggtcatctg cgggttgtcg agccagaacg agaccttcga
    22741 gaccccctgg tccggtccgg tgaacacgta ggcgtccccc cggagggtct ggccctcgag
    22801 gtcggcgaac gtgctgcggt cggcggtggg gctcaccagc aggcggtaac cggtccccgg
    22861 cgcctggacg accagggtca cggggagcac gaccgatccg aagcccgccg tggtcgccgt
    22921 gacggtgccg ttgtacgtgc cggccgccag cccggccgtg tctccggtca ccgtgatggc
    22981 ctggggtgtg gtccccgagg tcggctgagc gctcagccag ggccgatcgg tggacagcgt
    23041 gaacgccgtc tgcgtgccgt cggtgacctc gagggccgcc gtggcgctcg gcgcgtcgcc
    23101 ctgctcgccg gagaggccga ggctcgtcgg ctggaagcgg agctgggggg cggtgttcgc
    23161 gaccgtgaac gtcgcgtgga ccacctcgct ccctccgccg gaaagctcga cgagcgcgct
    23221 gatcgtgtgc gagccgtcgg acacgctcga tgtgttccat gggaaggccc taccgtcctg
    23281 cgccgtccct gcgaagtcgt agggcgcacc cttctccacc ttcgtcgggg tgcccgtcat
    23341 ggcggggtta tcgagccaga aggagacctt cgagaccccc tgctccggtc cggtgaacac
    23401 gtacgcgttc ccttggaggg tctggccctc gaggtcggcg aagccgctgc ggtcgggcga
    23461 cgggctcacc agcagccggt agctgctccc catgagaccg acgctcacgc tgacaggtag
    23521 cgtgagcgag tcgaggcctg gagcgcccgc cgtcacctct ccggtgtgcg tgccgagcgc
    23581 caggccggtc gtgtccgcgg tcaccgtgat ggatcgtggc gtcgacccgc tggctggcag
    23641 cgccgtcagc cagggccggt tcgtcgagag cgcataggtc gccgccgagc cgtcggtcac
    23701 gctcaggtcg acgatggcgc tcggagattc cccctgctcc cccgacaggc tcagcgcccc
    23761 gggttggaac cgcagctgcg gctcgaggtt cgagaccacg aacgtcccgt gcaccacctc
    23821 gacggctccc ccggagagct cgagccgcgc ggtgatcgtg tgggacccat ccgccatctt
    23881 cgtcgtgtcc catgggagcg ccgctccgga tccggctgtg ccggccaagt cgaagggggg
    23941 gttgttctcc gtcttgaacg ccttcgtgct catgctcggg tcgtcgagcc agaacacgac
    24001 cctggccacg ccctcatccg gccccgtgaa cacgtacgcg tccccctgga gggtccgtcc
    24061 ctcgaggtgc gcggggctgc ttcggtcggc cgatccgctg acgaggaggc ggtagccgcc
    24121 caccgccgtc accttgaacg tcaccggcac gtctacgggc tcgtaacccg gtgccgtcgc
    24181 cgtcagcgtc gcctggtgcg tgcccggtgc gaggccggat gcatcggcct gaaccgacag
    24241 ggacgcgggc gtggatccgg aggcggggac ggcggtgagc cagggagcgt cggtccctgc
    24301 ctcgaacgac gccgccgtgc cgtcgctcac ggtgagagcg accgtcgcac cggccacctc
    24361 gccctcgacc gcggtgaaat tgagaacgcc ggggctcgtc ttcagctcgc cggggcaggt
    24421 gccgtcccga tactcgaagg cgtcgatgac cgtcggggag ccgccatccc ccgtgaagcc
    24481 gtaggtctcg acgcggagag cgccctcgtc ggacaccgcc agcctcgtga agtggtgcag
    24541 cgtgttgtct cggaaggccg tcgagtgcgg gggcgggttc gtcgcgaact gcgagaagtc
    24601 gccgttcttg ttgctcagct tcccggcggg gctgaccttc acgtacgtca cgccatcgtc
    24661 catcgtgtag caggacaagg acgacgaggt gggcgtgttc gtggcgggga cgtcggtgag
    24721 cgggtacgtc cgctcgtagg actgatcgtg ggaggtcagc acgacctgca ccccgagctg
    24781 ctcgaagagc ggaccgagct gtccccgaag gttgaggttc gacgggtggt tcttcccgtc
    24841 ggagaacgac ggcacgtgca tgtacggaac gatccagcgc tggccagccg ccttcgccgc
    24901 ctcgatgtcc tgacggatcc agtcgagcgc ctgtgacgag agtccctgcg tctccgcgac
    24961 cgccaggatc gagacgaagt ggacgtcccc gacgtcaaag gagtagaacc ggccgccgtc
    25021 gaacccctgg ggggtcgcga atctcgcgga ccacgccgtg tagctctcgc ccaggagcac
    25081 ctcgtggttc ccgtacgtgg gcatcatcgg agcccccgtg gcgaagggct cgttctgcgt
    25141 gaaccacgcg tcgatcgagt cctcgagcga cccgtaccgc ttgtcggtgt cgtagtagat
    25201 gtagtcgccg ccggggagga acagcgtcgg atccagggcg gccatctcct cgatcgcctg
    25261 ctcggtcccc gtggccaggc cgtccccacg accgataagc ccggtgtcgg cgaagtagat
    25321 cgccgagaag tccgcggggc ccggcgcggg agccgtacgc gtcgtgtgca cggggctcca
    25381 gccctcgctg ccgccgttca cccggtactc gtacgtcgtg gccggctcga ggccggtgat
    25441 cgtggcctcg cggagcgtcc cgttcgtccc cgaggtccgc gtcccgccgg aggcctgctg
    25501 ccacgcggag gcgccctggc tccgatactc gacgaccgag gaggcgtcag gggccaaggt
    25561 ccgccacacc accgtcatcg tggtggatgg gtcatcgacc cacgacaggt ggatctggtc
    25621 ggccgggccg acctccccga ccgtcagggt cacgtcgaag gtggccggag tgtgaccgtc
    25681 ggcggaagcg gtgaccgtcc ccccgtacac cccgttcgcg agaccggtgg cgtccaccga
    25741 caccgtgagc gtagccggca cgtttcccga agccggggtc gcggagagcc acggcgcgct
    25801 gctcgtcacc tggaaggccg cggaggtcgc ctcggacgaa gacacagccg tactggccga
    25861 agccgtacct cccgggtcga cggcgagatc caccgacgtg ggggagaagg agagctgggc
    25921 gtccgcgttc tgcaccgtga acgtggcgtg cagaacgtcc gtccctcccc cgctcagatc
    25981 cacctgcgcg gagatcgtgt ggaggccgtc gccgacgccg ttcgtgtcga acggcttagc
    26041 ggtgccgtcg ctcgttacgg tgccgtcgaa gtcccacggg gctctccgct cggtcgtcct
    26101 cggcttccca ctcatcgagg ggttgtccag ccagaagcgg acctgcatca cgccggactc
    26161 cggggccagg aagacgtaga tcgagtcctc gacggtggac ccgtgcaggg cgctgccccc
    26221 cgagcgatcc ggggcgacgc tcacgagcag ctggtaagcg gactgcgcct gcgaggcagg
    26281 ggccagcgag gtcatggtga gggccagacc gagcaggagc ccgcccaccc tccccagtga
    26341 tcggtggccc cgataccgag tgcctgccgg ttgctgtttc atcgtcaatc tggccccctg
    26401 ggacatcgtt cggcgtcgac acactctcac gggcaacatc ggtggggaag ggctatctcg
    26461 ggatcgtcga tcgtccctgc atctgacttg gtcctgacgg ctccggaccg acctggacca
    26521 ggccgtcgtt ccgagccgcc acctgttcga aggtgccggc gtcgacgacg cggccgtccc
    26581 cgatcaggac gatccgatcg cagccccgca cggtcgagag ccggtgggcg accatgatga
    26641 tcgtccgacc cgccctcgac ctatgcaggg cctccatgaa tgcggcctcc gtgcggttgt
    26701 cgagcgcgga ggttccctcg tcgaggatca gcaccggcgc gtcccggtac agggctcggg
    26761 cgatcgccac gcgctgccgc tggccgcccg agacccgaac ccctcgctcc cccaccagag
    26821 tgtccaggcc gtccggcaga ctgtggacga actccgagag ctgagccacc tccaccgctg
    26881 cctccagtct cgaggggtcg atctcggagt cggccacgcc gagggcgatg ttctggcgga
    26941 tgcttccgtc gatcatgaac accgcctgag agaccacccc gagctccctc tgccaggcgc
    27001 gctcccggcc ctccaatggc acaccgtcga ccaggacctc cccgctgacg ggccggagga
    27061 gcccggcgat gatgtccagg agcgtgctct tccctccccc tgtcggcccg acgatcccga
    27121 cggcctcgcc ggcagcgatc gtaaggttca cctcggcgag cgccgcttcg gggcggcccg
    27181 ggaaccagaa gctgacgtcc acgagcgaca gctcccggcg gaactccagc gaaggctgat
    27241 cctccttctc ctcctccgcg cgggcctcgg tcagcaggat gtcttcgtag gcgtgctcca
    27301 ccgccctgct ggcgaagcga agggaggtca cgctctgcac gatccgctgg agcgccggct
    27361 gcatccgcag cgccacgtag gcgaacatcc cgaggaccgc cagcagctgc cccacccctc
    27421 cctccagggc caggaacacg atgaagaaca ccgcgatgaa aaccacgagc accgtctcga
    27481 tcaggatcga ggggaactcc atcccgacgc ggcccaggta cgtgaccctg gccatgtccc
    27541 ggcgctggcg caggtacacc cgggagaaga agggcaccgc gtcgagcagc ttcacgtccc
    27601 ggatcccgtg gagcgcctgc tgcacgaccg agaggttcgc cttggccagg tgctgacgcc
    27661 gcccacccca ccggcggagc cgaggctgaa ccaccttcag cagcagaagg accaccggag
    27721 cgagggtggc cacggcgaag ccggtcgcca gcggcgcggt gacgaccagc acgccgagca
    27781 tcgccacggc caggacggaa ccggacaaga gccggacgat ggggcccagg acgtcgagcg
    27841 tgatcgccct caccgtctcc tcggtgttcc ggacgagctc ggccgagctg cgctcgaggt
    27901 ggaacggata gggcatccgc aggtacccgg tgaagagccg ctcggccagc ctggctccca
    27961 cgttctggga gatccggctc tggacgtaca gctggagcag cgtgatggcg cctcggagga
    28021 ggaagaagac gaccaccacg cccgcgaccc aggacatcag cgtctcctgc ggaacgtggg
    28081 agaacctcga cgagatgtcg ccgatcactg gaagctcgat ccggccgccc ggggcggtga
    28141 tgaggccgat caacaggaag atcaggatgg cggcggcggc ctcgactccg ctcgcgacga
    28201 ccgccagcgc gatcacgccg gcccactgtc gcaggccctc tggccgaaga aggtggatca
    28261 gcttccgaat ggtgtccgtc atgggttccc caccatctct tgcggtgcgg tcgcatcccg
    28321 gtcgtcggag cggtgtcccc gggagcggcc ctgggacatc cagcggagcg gcgtcgccca
    28381 ccgcctgcgg tacgaggtgg gaccgccttc gctcgatcgg ctttggagga actcccggct
    28441 ggggaagagc agggcgatca ggtacgccgc cttggcccga aggcctcgga tcgcccggag
    28501 cgatcccagc gacttccctc ctcggtcccg tcgtgccgtc gtgtacgcgt ccagcgcccg
    28561 gcgctcgcgg cgcgaggggc gttcctccgt gccgggagca agaccctcgg gcagtcgcac
    28621 gcccaccgac tccgacagca cccgcgaggc gtgccggtag acgacagaca gccgccagcg
    28681 ccgaccccag tccgcgagcc gggcccgatc gaccccactc gacgccgcct gggccacgtc
    28741 cctcagcgga aggaggaggg gcgggcggtg accgagggcc gcgtgcacga aggcgtgcat
    28801 gagcagcgcc gtgtcgtcga gccgccggag cgtgcggccc cccagctcga acgtctccgt
    28861 gctgtcgaac agctcgtcgg ggtccatcca gaggccgaac ggccccccaa ccagcgtccg
    28921 gtgaaggtcg acctcgaggc ccgccggccc ttggtgtgcg gcggtgtgcc cgaaccgctc
    28981 cacgaatccg ggacgcggct cggggaaccg tcgtcggtag ccgagctcgg gcaggagcga
    29041 gcaggcccgc cgccaatcag ccgtccgcac cagaacgtcc aggtcgccga aggggcgcca
    29101 cgagggatca ggatagatgg tgtgggcgac ggccggcccc ttgagaacga tgagctcgac
    29161 gccggcgtcc gcgaaggcgc gggccagccg cagcagctcc cgctcgaggg ccagcgcgtg
    29221 acacatcgcc tcctgctgcc ggtcgaggag ctccgcctcc tcctcctccg agagcttcag
    29281 cgctccctcc ttcaaagcgg ccgccgcgat gccggtgagt cgctccctca cgagcccgct
    29341 gagggccctg ggccacccgc cgtccggaac ggccaaggga tcggagggcc cgcccgggac
    29401 accgaacgac gccagctcac ggaggaaggg ctctaccggc cgccagtcag ccacggctcg
    29461 cgtcgacctc ctcgatgaga cctcgctgcc tcagatcggc caacagcccc tcgacgtcct
    29521 gcgcgatcac gtcgcgcgga ccctcgaacc atgccgcgag cgtcgccgtg atctcgcccg
    29581 gcgtgaccgc ttcctcaagg aggtcccaga tcatggcgcc cgccccgtcg agcacctcga
    29641 agccttctcg gtcccccgcc gtgaggatca ccctctcgcc aaccgatcga gcgagcacgg
    29701 tgggcgagtg ctgcagccgg ggagcgtcgc tcacagccgg accacgttcg tcggcacgct
    29761 cgccccgaag gcgttctgcg cgtcgaagat gagccgagcg tgctccgcga tccattccag
    29821 gtcgtacgcg ctgtggggtg tgagcagcgc cacgcagtcc gccgcggtga cgctccgaag
    29881 ggtcagctcc gtccggcgga gcacctcacc gttcagggtg aacgactcca cgtagggatc
    29941 gtggaacgag agcttggcct tcgtcctgac gagatgcttc atgacgttga cggccgggga
    30001 ctcccggacg tcccccacgt cgggcttgta ggccacgccc aggaccagga tgctcgactg
    30061 acgcaggggc ttgccctctt cgttgagggc ctcgccgatc cggcgcgcga cgtacctggg
    30121 catctgggcg ttgacgtcct cggcctgctc gagtatccgg aactggtggc cggcgtcgcg
    30181 gcgcacctgc cacgccaggt aggtcgggtc gagcgggatg cagtgcccgc cgacgccggg
    30241 tccgggcgag aagggcatga atccgaaggg cttcgtggcc gtggcctcga tcacctccca
    30301 gacgtcgatc tccatctcgt ggcagagcgt cgccatctcg ttgaccaggg cgatgtttac
    30361 gtgccggaag gtgttctcca ggagcttcgc cagctcggcc gcccgcgtgg aagagaccgc
    30421 gaagaccttg tccacgagct gctcgtagaa gagcgtggcc agtcccgccg cctccggggt
    30481 cacgcccccc acgacgcgcg ggatgttccg catcccgtac tcctcgttcc ccgggtcgat
    30541 ccgctccggc gagtacgcga ggaggaagtc ccggtccgcg aagaaccccg aggtctccag
    30601 gatgggtctc acgagctcct cggtggtccc cggatacgtc gtggactcga gcacaacgag
    30661 gcagcccggg ccgagctctg ccgccacctt ctcgcaggcg ttctcgatga acgacacgtc
    30721 cggggtgttg tcccgcacag gggtcggcac gcagatcagg atcacgccgt ggtcggcgag
    30781 ctgcgcgtgc tccgtcgaga agctgaggcg gccggtggcc atcgccgcgc ggaacatctc
    30841 ctcggacacc ccagcg
//
LOCUS       DASUTW010000112         4342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_551842, whole genome shotgun sequence.
ACCESSION   DASUTW010000112 DASUTW010000000
VERSION     DASUTW010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551842"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VF058_03635"
     CDS             complement(<1..372)
                     /locus_tag="VF058_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207430.1"
                     /translation="MDDLRTARDRSDRRDPPSPRSMAVPWALLVIWGVVASVLATWLY
                     LDREAATEELASARVEVTELRRLVVEAEGRIDELREGFQDQQDEVTRRGESVEACREA
                     LRRISVAWSGVAQAVRAAGALD"
     gene            523..1680
                     /locus_tag="VF058_03640"
     CDS             523..1680
                     /locus_tag="VF058_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX6207431.1"
                     /translation="MEEDAQQVEEQGPEAAAPESIVDEFNTWLGHLVGSDGSDLHIKV
                     GSAPMIRLRGILRRVEREPLTAGHTQALVAAIVPPDRRRHLDDEGEVDFAHSLPGVGR
                     FRVNAFRQRGSISIVLRKLRFGGPEFAEIGLPEVVRRLSDEHRGLILVTGPTGSGKTT
                     TLSAMIEHINKTRPVHIVTIEDPIEVMFEDKVASINQREVGLDTQSFLGALRVALRQD
                     PDVILIGEMRDSETVRTALQAAETGHLVLSTLHTLDATETVNRVVDFFPPHQQQQIRF
                     TLAGSLRGILSQRLLPAIDSDRVPAVEVLVNTGRVQERIIDASMTAEIREVIRDGEYY
                     GMQTFEQSLLQLVQQGKVAVEEAMVATTQPHDFRLMLEQAGITEVGALKTV"
     gene            complement(1803..2156)
                     /locus_tag="VF058_03645"
     CDS             complement(1803..2156)
                     /locus_tag="VF058_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207432.1"
                     /translation="MTSRSIVEWTTNDFPAAVDLAHRISDYVEANEPETLVLEWFGNE
                     NTGKVVWYQVYSNDEAFLTHAQNMIEAGFRDEARQLFTQARLLLLTPPTHPRAQEMAR
                     QLGAEQVMPIGGVVR"
     gene            complement(2214..2286)
                     /locus_tag="VF058_03650"
     tRNA            complement(2214..2286)
                     /locus_tag="VF058_03650"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2251..2253),aa:Asn,seq:gtt)
     gene            complement(2340..3695)
                     /gene="rpoD"
                     /locus_tag="VF058_03655"
     CDS             complement(2340..3695)
                     /gene="rpoD"
                     /locus_tag="VF058_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002563567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX6207433.1"
                     /translation="MADPARDPVLDEAKERIAQRGRERGFVTSEDLLEDVPVEDLRPE
                     QVEEFLAGIEEHLREQGIEVIEVPGEEVSAEAQASLAREEDVLKAPTNDPVRMYLKEI
                     GKVPLLTAPEEVDLARRIEVGEFATELHRLAVEIELNGTELAPADARRSKAEVDKQFR
                     RVVQLVCEIREHQEEKFGRVHAIGRESVQRSWKPPSAQVRDEFIRRVERDGQLAKKKL
                     IEANLRLVVSIAKRYVGRGMLFLDLIQEGNLGLIRAVEKFDYSKGFKFSTYATWWIRQ
                     AITRAIADQARTIRIPVHMVETINKLIRIQRQLLQDLGREPSPEEIGKEMGIPAEKVR
                     EILKVSQEPVSLETPIGEEEDSHLGDFIEDAEAIAPVDAASFILLQEQLESVLHTLSE
                     REKKVIELRFGLMDGHPRTLEEVGREFGVTRERIRQIESKTLSKLRHPSRSQKLRDYL
                     E"
     gene            complement(3698..>4342)
                     /locus_tag="VF058_03660"
     CDS             complement(3698..>4342)
                     /locus_tag="VF058_03660"
                     /inference="COORDINATES: protein motif:HMM:NF019883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207434.1"
                     /translation="ALPIVAGLDDLVRREQYAGMLADLTGVTTSTVLLELERRGDRPE
                     RPQWSERSERSEPRVTRRPGTGQPRTPTQELEKEALKLLAQAPEAARPVAAPLDPDHF
                     ETDRYRRVFAILRDAGGDAARLVERGGEEGLGELVAQLSVEPLTVEPTREYAERVFSR
                     LEEVFLGRKIATMKRRLEKLNPTDDPSAYDSLFEELIALESQRRRIRTRAGEGV"
BASE COUNT          679 a         1506 c         1446 g          711 t
ORIGIN      
        1 gtcgagcgct ccggccgccc ggacggcctg cgccacgccg ctccacgcga ccgagatccg
       61 ccgcagggcc tcccggcacg cctcgacgct ctcccctcgc ctggtcacct cgtcctgctg
      121 gtcctggaag ccttcccgaa gctcgtcgat ccttccctcg gcctcgacga ccaggcggcg
      181 gagctccgtt acctcgaccc tcgccgacgc aagctcttcg gtcgcggcct cccggtccag
      241 gtagagccag gtggccagga ccgaggccac gaccccccag atcacgagca gcgcccacgg
      301 gacggccatc gatcggggac tcggcgggtc gcggcgatcg gaacggtccc gggcggtgcg
      361 gagatcgtcc atggcacgcc gaagcatagg ggcgggggtc gaaacgctca agaaggccgc
      421 cccacggtcc gatgggatgg gagtgaggcg ggcgcacgac gtcccgccgt ccgaggctcg
      481 cgagagcagg gggctcgatc cgtcatgaca tccgatatgg gaatggaaga ggacgcgcag
      541 caggtcgagg agcagggacc cgaggccgcc gcgcccgaga gcatcgtcga cgagttcaac
      601 acctggctgg gccacctcgt aggcagcgac gggtccgacc tccacatcaa ggtggggtcc
      661 gccccgatga tccgcctgcg cggcatcctc cgtcgcgtcg agcgcgagcc gctcacggcg
      721 ggccacacgc aggcgctcgt ggcagcgatc gtcccccccg accggcggcg ccacctcgac
      781 gacgaaggtg aggtcgactt cgcgcactcc ctccccggcg tgggacggtt ccgcgtcaac
      841 gccttccgcc agcgcggctc gatcagcatc gtgctgagga agctgcggtt cggggggccc
      901 gagttcgccg agatcgggct gccggaggtg gtccggcgcc tctccgacga gcaccgcggc
      961 ctgatcctcg tcaccggccc gaccggctcg gggaagacca cgacgctctc ggcgatgatc
     1021 gagcacatca acaagacccg gccggtccac atcgtcacga tcgaggaccc gatcgaggtc
     1081 atgttcgagg acaaggtagc ctcgatcaac cagcgggagg tcgggctgga cacccagtcg
     1141 ttcctcggcg ccctccgtgt ggcgctgcgt caggaccccg acgtgatcct gatcggcgag
     1201 atgcgggact ccgagaccgt gcggacggcg ctgcaggccg cagagaccgg gcacctcgtg
     1261 ctctcgaccc tgcacaccct cgacgccacc gagacggtca accgcgtcgt ggacttcttc
     1321 cccccgcacc aacagcagca gatccggttc acgctcgcgg gatcgctgcg cggcatcctc
     1381 tcccagcggc tcctccccgc gatcgacagc gaccgggtcc ccgcggtcga ggtgctcgtc
     1441 aacaccgggc gggtccagga gcggatcatc gacgccagca tgacggcgga gatccgcgag
     1501 gtgatccggg atggcgagta ctacggcatg cagaccttcg agcagtcgct cctccagctc
     1561 gtgcagcagg ggaaggtcgc ggtcgaggag gcgatggtcg cgacgaccca gccgcacgac
     1621 ttcaggctga tgctggagca ggcgggcatc accgaggtgg gcgctctgaa gacggtctga
     1681 gcctgagagc tccgggggcg agactcgaac tcgcaaccca ccgggcaacg gagcgactgc
     1741 tcgccgcttc tctgttccct ggaacgcagg ggcgagggaa cctcgttgtg ttcagacgcg
     1801 agctatctga ccacaccgcc gatcggcatt acctgctctg ctcccagctg acgtgccatc
     1861 tcctgagccc gcggatgtgt ggggggagtc agtaagagaa gccgggcctg ggtgaacaat
     1921 tgccgggctt catctcggaa tccagcttcg atcatgttct gggcatgggt caggaaagct
     1981 tcatcgtttg aataaacctg gtaccagacc accttgcctg tgttctcgtt tccgaaccat
     2041 tccaagacga gcgtttcagg ttcgttggct tccacatagt cagagatacg gtgagcaagg
     2101 tcgacagctg caggaaagtc attggtggtc cattcgacta tcgatcgaga tgtcaccgtt
     2161 gcttctcctt actacctcta ggtcaacccg ggccagggta acctgctctt tcgctccggg
     2221 ggggagactc gaactcccaa cctaccggtt aacagccggc cgctctaccg attgagctac
     2281 cccggaagac gagcgcgtga agcgcccgaa agcaaagtgt accagcggcc gccgtgccct
     2341 tactcgaggt agtcgcggag cttctgggag cggctggggt gccggagctt ggagagggtc
     2401 ttcgactcga tctgccggat gcgctcgcgc gtgacgccga actcgcggcc cacctcctcg
     2461 agcgtgcgcg gatggccgtc catcagcccg aaccgcagct cgatcacctt cttctcgcgc
     2521 tcggagagcg tgtggaggac ggactcgagc tgttcctgga ggaggatgaa gctggccgca
     2581 tccaccgggg cgatcgcctc ggcgtcctcg atgaagtccc cgaggtgaga gtcctcctcc
     2641 tcgccgatcg gggtctccag ggacacgggc tcctgggaga ccttcaggat ctcgcgcacc
     2701 ttctcggcag ggatgcccat ctccttgccg atctcctcgg gggagggctc gcggcccagg
     2761 tcctggagca gctggcgctg gatccggatc agcttgttga tcgtctccac catgtggacg
     2821 gggatgcgga tggtgcgggc ctggtcggcg atggcccggg tgatggcctg gcggatccac
     2881 cacgtggcgt aggtggagaa cttgaacccc ttcgagtagt cgaacttctc cacggcccgg
     2941 atcaggccga ggttcccctc ttggatcagg tcgaggaaca gcatcccccg gcccacgtac
     3001 cgcttggcga tggagacgac gagacggagg ttggcctcga tcagcttctt cttggccagc
     3061 tggccgtcgc gctccacccg ccggatgaac tcgtcccgca cctgggcgct gggcggcttc
     3121 cacgaccgct ggaccgactc ccggccgatc gcgtgcacgc ggccgaactt ctcctcctgg
     3181 tgctcgcgga tctcgcagac gagctgcacc acgcggcgga actgcttgtc cacctcggcc
     3241 ttggacctcc tcgcgtcggc cggcgccagc tcggtcccgt tgagctcgat ctcgacggcg
     3301 agtcggtgca gctccgtggc gaactccccc acctcgatgc ggcgggccag gtcgacctcc
     3361 tcgggagcgg tcaggagcgg gaccttgccg atctccttga ggtacatccg gacggggtcg
     3421 ttggtgggag ccttgaggac gtcctcctcg cgagccagcg aggcctgggc ctcggcggag
     3481 acctcctcgc cggggacctc gatcacctcg atcccctgct cgcggaggtg ctcctcgatc
     3541 cccgcgagga actcctcgac ctgctcgggg cggaggtctt cgactgggac gtcctccagg
     3601 aggtcctcgg aggtcacgaa gcctcgctcc cggcctcgct gagcgatgcg ctctttcgcc
     3661 tcgtcgagaa cgggatcgcg cgcaggatcg gccacgttca gactccctcc ccggctcgtg
     3721 tccggatccg ccgccgctga gactccagtg cgatcaactc ctcgaacagg gagtcgtacg
     3781 cgctggggtc gtcggtcggg ttgagcttct ccagacgcct cttcatggtc gcgatcttcc
     3841 gcccaaggaa cacctcttcc aggcgggaga agacccgttc agcgtactct ctggtcggct
     3901 cgaccgtcag cggctcgacc gagagctgcg cgacgagctc tccgagcccc tcctcgcctc
     3961 cacgctccac gagacgggcg gcatcgcccc cagcgtcccg caggatggcg aacactcggc
     4021 ggtagcggtc ggtctcgaag tggtcggggt cgagcggcgc ggcgaccggc cgggccgcct
     4081 cgggggcctg ggccaggagc ttcagcgcct ccttctccag ctcctgggtg ggcgtgcgcg
     4141 gctggcccgt cccggggcgg cgggtcaccc ggggctcgga ccgctcggac cgctcggacc
     4201 actggggccg ctccggccgg tcgccgcgcc gctccagctc gagcaggacg gtcgaggtcg
     4261 tgacccccgt gaggtccgcc agcatccccg cgtactgctc ccgacggacg aggtcgtcca
     4321 ggccggccac gatcgggagg gc
//
LOCUS       DASUTW010000113         2076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_551882, whole genome shotgun sequence.
ACCESSION   DASUTW010000113 DASUTW010000000
VERSION     DASUTW010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551882"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VF058_03665"
     CDS             <1..636
                     /locus_tag="VF058_03665"
                     /inference="COORDINATES: protein motif:HMM:NF019883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207435.1"
                     /translation="ALPIVAGLDDLVRREQYAGMLADLTGVTTSTVLLELERRGDRPE
                     RPQRSERSEARVTRRPGTGQPRTPTQELEKEALKLLAQAPEAARPVAAPLDPDHFETD
                     RYRRVFAILRDAGGDAARLVERGGEEGLGELIAQLSVEPLTVEPTREYAERVFSRLEE
                     VFLGRKIATMKRRLEKLNPTDDPRAYDSLFEELIALESQRRRIRTRAGEGV"
     gene            639..1994
                     /gene="rpoD"
                     /locus_tag="VF058_03670"
     CDS             639..1994
                     /gene="rpoD"
                     /locus_tag="VF058_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX6207436.1"
                     /translation="MADPARDPVLDEAKERIAQRGRERGFVTSEDLLEDVPVEDLRPE
                     QVEEFLTGIEEHLREQGIEVIEVPGEEVSAEAQASLAREEDVLKAPTNDPVRMYLKEI
                     GKVPLLTAPEEVDLARRIEAGEFATDLHRLAAEIELNGTEVAPADARRSKAEVDKQLR
                     RVVQLVCEIREHQEEKFGRVHAIGRESVQRSWKPPSAQMRDEFIRRVERDGQLAKKKL
                     IEANLRLVVSIAKRYVGRGMLFLDLIQEGNLGLIRAVEKFDYSKGFKFSTYATWWIRQ
                     AITRAIADQARTIRIPVHMVETINKLIRIQRQLLQDLGREPTPEEIGKEMGIPAEKVR
                     EILKVSQEPVSLETPIGEEEDSHLGDFIEDAEAIVPVDAASFILLQEQLESVLHTLSE
                     REKKVIELRFGLMDGHPRTLEEVGREFGVTRERIRQIESKTLSKLRHPSRSQKLRDYL
                     E"
BASE COUNT          355 a          704 c          740 g          277 t
ORIGIN      
        1 gccctcccga tcgtggccgg cctggacgac ctcgtccgtc gggagcagta cgcggggatg
       61 ctggcggacc tcacgggggt cacgacctcg accgtcctgc tcgagctgga gcggcgcggc
      121 gaccggccgg agcggcccca gcggtccgag cggtccgagg cccgggtgac ccgccgcccc
      181 gggacgggcc agccgcgcac gcccacccag gagctggaga aggaggcgct gaagctcctg
      241 gcccaggccc ccgaggcggc ccggccggtc gccgcaccgc tcgaccccga ccacttcgag
      301 accgaccgct accgccgggt gttcgccatc ctgcgggacg ccgggggcga cgccgcccgt
      361 ctcgtggagc gtggaggcga ggagggcctc ggagagctca tcgcccagct ctcggtcgag
      421 ccgctgacgg tcgagccgac cagggagtac gctgaacggg tcttctcccg cctggaagag
      481 gtgttccttg ggcggaagat cgcgaccatg aagaggcgtc tggagaagct caacccgacc
      541 gacgaccccc gcgcgtacga ctccctgttc gaggagttga tcgcgctgga gtctcagcgg
      601 cggcggatcc ggacacgagc cggggaggga gtctgaacgt ggccgatcct gcacgcgacc
      661 ccgttctcga cgaggcgaag gagcgcatcg cccagcgagg ccgggagcga ggcttcgtga
      721 cctccgagga cctcctggag gacgtccccg tcgaggacct ccgccccgag caggtcgagg
      781 agttcctcac cgggatcgag gagcacctcc gagagcaggg gatcgaggtg atcgaggtcc
      841 ccggcgagga ggtctccgcc gaggcccagg cctcgctggc ccgagaggag gacgtcctca
      901 aggcccccac caacgacccg gtccggatgt atctcaagga gatcgggaag gtcccgctcc
      961 tgaccgctcc cgaggaggtg gacctggccc gccgcatcga ggcgggggag ttcgccacgg
     1021 acctgcaccg gctcgccgcc gagatcgagc tcaacgggac cgaggtcgcg ccggccgatg
     1081 cgaggaggtc caaggccgag gtggacaagc agctccgtcg cgtggtgcag ctcgtctgcg
     1141 agatccgcga gcaccaggag gagaagttcg gtcgcgtgca cgcgatcggg cgggaatcgg
     1201 tccagcggtc gtggaagccg cccagcgcgc agatgcggga cgagttcatc cggcgggtgg
     1261 agcgcgacgg ccagctggcc aagaagaagc tgatcgaggc caacctgcgg ctcgtcgtct
     1321 ccatcgccaa gcggtacgtg ggccggggga tgctcttcct cgacctgatc caggagggga
     1381 acctcgggct gattcgggcc gtggagaagt tcgactactc gaagggcttc aagttctcca
     1441 cctacgccac gtggtggatc cgccaggcca tcacccgggc catcgccgac caggcccgca
     1501 cgatccggat ccccgtccac atggtggaga cgatcaacaa gctgatccgg atccagcgcc
     1561 agctcctcca ggacctgggc cgcgagccca cccccgagga gatcggcaag gagatgggga
     1621 tccccgcgga gaaggtccgc gagatcctga aggtctccca ggagccggtt tcgctggaga
     1681 ccccgatcgg cgaggaggag gactcccacc tcggggactt catcgaggac gccgaggcga
     1741 tcgtcccggt ggatgcggcg agcttcatcc tcctccagga gcagctcgag tccgtgctcc
     1801 acaccctgtc cgagcgggag aagaaggtga tcgagctgcg gttcgggctg atggacggcc
     1861 atccgcgcac gctcgaggag gtgggccgcg agttcggcgt cacccgagag cggatccgcc
     1921 agatcgagtc gaagaccctc tccaagctgc gacacccatc gcggagccag aagctccgcg
     1981 actacctcga gtaggctgag gaccgccgct ggtacactct cctttcgggc gcccgtcgcg
     2041 cccgtcttcc ggggtagctc aatcggtaga gcggcc
//
LOCUS       DASUTW010000114        14771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1070493, whole genome shotgun sequence.
ACCESSION   DASUTW010000114 DASUTW010000000
VERSION     DASUTW010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14771
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070493"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VF058_03675"
     CDS             complement(<1..889)
                     /locus_tag="VF058_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207437.1"
                     /translation="MRSSIRWSVLVISAASLVLLGLPATAGHETDPRTNNLHPMGHIV
                     EPASLLNPAVGNPDIHTDIAFWGKHAFQGTWLGFNIRDISAPGNPKQVSHTVCAGDQG
                     DVVVWDDVLVRSWNSPASGSQQCDGQPVPAGFEGLHVFDISDLSDPVLVASVETECGS
                     HTASGVPDVANERLLIYNSSSSGACPGIDIVEVPLDAPQDAAFLRFEDAGRSCHDTGV
                     ILGDAMMAACAGGNGFTVWELDPADLEDPDFLYSKNVNDEAEIDGVTIGHSAAFSFDG
                     ETIVFGHEPGGGVAPRCRES"
     gene            complement(1174..2514)
                     /locus_tag="VF058_03680"
     CDS             complement(1174..2514)
                     /locus_tag="VF058_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207438.1"
                     /translation="MRPRFRWSVIVFATLALAATALPAGAEHKTYPQTNSIHALGHSE
                     NPGFFSLDPGERDNVNSDLAFWGKTAIQGRYNGFRIIDISAPGNPKLVGEAFCDGDQG
                     DVIVWENWVVRSWNAPARPDVECGGEPVPEGFEGFHVFDISDVSNPVLVDSVELSARP
                     EADAFGCGSHTATGVPDLENGRLVIYNSTSGGPCPFIGVLELDLATGDLSYIRDEPLT
                     DAGASHDCGAILGDTMMLACASQAAANVFSIAGSLEDPDFQYTITEPGVGVGGNWHSA
                     AFTWDGEVIVLGWEPGGGGAAECEATDADVKKSMFFYDSETGAKLGQWTLERPQGADE
                     NCTIHNYNIVPLRSGRYVAVGGHYQAGTWVVDFTDPANAEAIAWADPESLGPGPFCGG
                     MCQIGGAWSSYWYSGAIYESDITRGLAVYKVSHRSLAGSIKVDHLNPQTQEFTL"
     gene            complement(2684..4693)
                     /gene="uvrB"
                     /locus_tag="VF058_03685"
     CDS             complement(2684..4693)
                     /gene="uvrB"
                     /locus_tag="VF058_03685"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX6207439.1"
                     /translation="MSSFRVQTDLRPAGDQPKAIDELTEGLAAGEDRVTLLGITGSGK
                     TFTAANVIERLGKPTLVISPNKSLAAQLANEFREFFPDSAVEYFVSYYDYYQPEAYVP
                     QTDLYIEKDATVNDEIDRLRHAATAALLTRKDVIIVASVSCIYGLGSPEEYEQQILHV
                     VRGRPVGHTAAMQRLVDMQYQRNQANLARGTFRVQGDTLEIHPSYQETALRVELWGDE
                     VERILEMDLITGEVVRELDHVTIFPATHYVASRDRLDRAIAGIEEELGVRLEQLERKS
                     KLLEAQRLRMRTNHDLEMLREVGFCSGIENYARHIDGRAPGTAPYTLIDYFPDDWLCI
                     IDESHVTVPQLHAMYEGDMSRKRTLVEHGFRLPSAVDNRPLKFDEFVERVNQVMFMSA
                     TPSDYELEVSKRVVEQIVRPTGLVDPEVEIRPTRGQVDDLMEKVRRRAEADQRVLVTT
                     LTKKMAEDLTDYLIEMGLRVRYLHSEIDTIERIQIIRDLRLGEYDVLVGVNLLREGLD
                     LPEVSLVAILDADKEGFLRSETSLIQTIGRAARNVEGKVLMYADEVTDSMRTAISETQ
                     RRRRIQLEHNREHGIDPQTIRKKVSDILLTMVEERPETAPKRRRRAPERPELPREELT
                     RLIESLQDEMHLAAKELKFEYAARLRDEVNELRKELRALDEIGVG"
     gene            complement(4718..5086)
                     /locus_tag="VF058_03690"
     CDS             complement(4718..5086)
                     /locus_tag="VF058_03690"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEX6207440.1"
                     /translation="MQERRFTLEEAEAELPRLRELLPRLREARRRVLASGERVRGVAR
                     RNGGGREGTDYWRAIEALRRGVEDLAGRGIVLRDAEGGLVDFPSEREGRTVYLCWRLG
                     EDRIGHWHDVDAGFSGRRPL"
     gene            complement(5086..5799)
                     /locus_tag="VF058_03695"
     CDS             complement(5086..5799)
                     /locus_tag="VF058_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX6207441.1"
                     /translation="METKAVFFDAGETLVHPHPSFPELFAEVLRDHGHEVDPEMVREL
                     AVGASQRFTETLRSDEHALWSTSADRSRRFWFSMYLGLLGDTGIEDPDGRLTDALYAR
                     FSDLESYRLHPDALPLLERLEAGGYPMGVISNFEEWLEGLLEKLAVTRFFPVRVVSGI
                     EGVEKPDPAIFRIALDRMGVAAEESVYVGDHPYFDVEAAGEVGMRPVLIDRRGLYPEA
                     DGVRITSLEDLPEAVGLRV"
     gene            complement(5820..6419)
                     /gene="coaE"
                     /locus_tag="VF058_03700"
     CDS             complement(5820..6419)
                     /gene="coaE"
                     /locus_tag="VF058_03700"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX6207442.1"
                     /translation="MLLVGLTGGLGAGKSTVARMLAERGAVIVDADELARRSIEPGTR
                     GHRKVLEVFGDEVEAPDGSIDRRALADVVFGDPEARRALESITHPEVFRMLAEEVERR
                     RDTGDVVVFDAPLIVETGVDTAMDLLVVVAVAPETQVERVVARGMDRVEAEARIAAQA
                     SQEEREAKADVILRNEGDLRDLERQVDALWDRLRRESRG"
     gene            complement(6430..7779)
                     /gene="rpsA"
                     /locus_tag="VF058_03705"
     CDS             complement(6430..7779)
                     /gene="rpsA"
                     /locus_tag="VF058_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX6207443.1"
                     /translation="MTPEELAKAIEESVKDLNDGDLVSGQVVRIDPDEVLLDIGYKSE
                     GVIPTKELSIRHDVDPSEEVSIGDELEALVLQKEDKEGRLILSKKRAQYERAWGRIEE
                     VMESGGTIEGPVIEVVKGGLILDIGLRGFLPASLVDLRRVRDLHPFVGQVLECKIIEL
                     DRNRNNVVLSRRAFLEESQSESRKKVLESLQPGERRTGKVSSIVNFGAFVDLGGVDGL
                     VHVSELSWRHVEHPGEVVSVGEEVEVEVLDVNLERERVSLSLKATQEDPWKEFERKYN
                     AGEVIEGEVTKLVPFGAFVRVADDIEGLVHISELSDAHVESADEVVNVGDRVRVKVVD
                     VDVSRRRISLSMRRVEGDAIITPEFEPEPDEVVPEAPAEAEPEPEEPVEEALQPEPEA
                     EPRAEAAPQAEAEPEEPEAEPEITPEEPPAAEDAGQDEEDVSLEDIVADLRRREGRQ"
     gene            complement(7964..10594)
                     /gene="polA"
                     /locus_tag="VF058_03710"
     CDS             complement(7964..10594)
                     /gene="polA"
                     /locus_tag="VF058_03710"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX6207444.1"
                     /translation="MGTERLFLLDGHSLSYRAFFALPPDLATTTGQVTNAVYGFTSML
                     IKLLAEEHPEYVAVAFDKGRPTVRLEQYAEYKAGRAETPDEFRQQLPLIREVLETLRI
                     PIIEVEGHEADDAIATIAERAAEKGLEVVIVTADRDFFQLVRPGIRVKFNRKGISDIV
                     DYDEKAVTERFGVPPDKYVELVALKGDTSDNIPGVPGVGDKTAAKLITEHGTVEEVLE
                     HAADLTPKLRAGIEEAGDDLLRNRELVRLVTDLDLDIEPQECRVGEWDQDQIRTLFNA
                     LEFRSLLERLQEVGEPRPAAADVELRIEEGDADAVATVLERPRPAAVALRATEGGPSA
                     IAVSAGDGRAVHAPLDGSLPEPLRAWLEAPDRPIWVHDAKELQAALLPIGVEAAGIAV
                     DTMLAGYLLDPAEASYPIGALSERYLGVDLVGEATETPEEDGQLALQEDDRGPLAAEA
                     AAVAILAPLMAERLERQGAADLLRDVELPLATVLARMQAAGVKIDVPYLQEMSESLGD
                     RMRTLESELYEHAGEEFNLNSPPQLRVILYEKLGLTPGKRTKTGYSTDAATLEKLRHQ
                     HPIVEALLSYRELAKLKSTYLDALPPLVSPATGRVHTTFSQVAAATGRLSSLNPNLQN
                     IPVRGEQGREIRKAFIAEEGRVLLVADYSQIELRVLAHLSGDEALGAAFAEDADIHSA
                     TAAKVFGLPPEQVDSELRRRAKVVNYGLAYGMNAYGLATRLGIPPDEAKEFIDAYFEG
                     FPRVREFLDHQVTMAAAEGFTQTILGRRRYLPELQSPNPRVRDLGRRMALNAPIQGSA
                     ADIIKLAMIRVDQRVVDLPATMVLTVHDELVLEVEEASVEEVAAAVREEMSSAYELSV
                     PLRVDVGSGPTWADATPEGH"
     gene            10620..11285
                     /locus_tag="VF058_03715"
     CDS             10620..11285
                     /locus_tag="VF058_03715"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX6207445.1"
                     /translation="MTAGGSHQEDYLEAIYEMAEEGIPTQQARIAEWLGITPASVSEA
                     VKRLVQRELVEVGADRRLVFTADGRALAETLVRRHRLAERFLVEVVGLPWYLSHEEAT
                     VWGRAISSAVEERFVAMLGDPATCVHGNPIPGTRRPVDQRDLRPLPALPEGASGRLER
                     LTEDLELDLDVMRFLQDSGLMPGAGLTVASVGPDGTRTLEVDGDRVALGPEIADNLWV
                     RTS"
     gene            11316..11759
                     /locus_tag="VF058_03720"
     CDS             11316..11759
                     /locus_tag="VF058_03720"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX6207446.1"
                     /translation="MSENRTPPGFTREQALATAGEIGRGTFLERIELEWLEVGLDRLV
                     ARIPVAGNTQPYGILHGGATAALCETVGSFGTSIHVGLDRRPVGVQLSVNHLRAVREG
                     HVTATGVPLHVGRTIALWEIRVEDDGGRLVAVGTLTLVIRDAVPG"
     gene            11756..12472
                     /locus_tag="VF058_03725"
     CDS             11756..12472
                     /locus_tag="VF058_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019009953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEX6207447.1"
                     /translation="MIGTLINVATVLAGTLAGTLFGDRLPERIRETVLHSLGLITLLV
                     GLDAGLHTFRAPLSETTRASAILVMGAVLVGGILGELLHIERGLNGVGDRLKARFGGG
                     QARFTEGFVVASLVFCVGPLTILGAIQDGTTGDFQLLAIKATLDGFAAVAFASALGWG
                     VGFSTITVLVYQGALSLTAATAASIFTDVVVEAMTAAGGVIIVGIGLRLLEIREVRVG
                     NLLPALVLAPVAFAVVERVG"
     gene            complement(12896..13621)
                     /locus_tag="VF058_03730"
     CDS             complement(12896..13621)
                     /locus_tag="VF058_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009439596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX6207448.1"
                     /translation="MVRLEGINKWFGENHVLRDVDLAVNPQDVLVLIGPSGSGKSTLL
                     RTINLLEEPQEGSVIWDGKDITDVRADVDDVRTEIGIVFQSFNLFPHMTALRNVTIAL
                     RKVLRLDDSAAEERAREQLDHVGLAEKADSYPSQLSGGQQQRVAIARALAMRPKLMLF
                     DEVTSALDPELVKEVLDVMQRLASEGMTMVIVSHEMGFAREVGNRLVFIDEGRILEEG
                     RPQEMFAEPQHERTRSFLSRIIA"
     gene            complement(13636..>14771)
                     /locus_tag="VF058_03735"
     CDS             complement(13636..>14771)
                     /locus_tag="VF058_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX6207449.1"
                     /translation="PPPPPRTREGAMKSRGERLRGWFAHRRRHAAHGAAEQNTLSLAV
                     LIVGATSLVIVLLGIPLVITARGWIPDATILESIQSGPATTLLFAAVAAGLVASAVGF
                     GIYRRMPTKPARKAAISGAALGVQAVVAAGLLLLVRSGENFAFLVRQNLRFEVLEGFL
                     PDFITAIKNTILLAFFGEVGGIVIGLILAMMALSQHAVVRAPARAYINFFRGTPLLWQ
                     LSFFYLGFVLGLGLAQTVTVFQAAVFIFALNAGAYTAEVFRAGIQSIERGQMEAARSL
                     GMTYFQAMRYAILPQAFRRVIPPLTNEFIILIKDTSLVTLLGLTADQRELLTWARDLY
                     AETFNATPWLAAAAGYMIVTLPMIRAVSWLERKLRSGLVGVGA"
BASE COUNT         2099 a         5352 c         5004 g         2316 t
ORIGIN      
        1 cggactcccg gcaccgcggc gccaccccac cgcccggctc gtggccgaag acgatggttt
       61 cgccgtcgaa gctgaacgcg gccgagtggc cgatcgtcac gccgtcgatc tccgcctcgt
      121 cgttcacgtt cttcgagtac aagaagtcgg gatcctcgag gtcggccggg tccagctccc
      181 acacggtgaa cccgttgccg cccgcgcacg ccgccatcat cgcgtccccg aggatcacac
      241 cggtgtcgtg gcaagaccgt ccggcatcct cgaagcgcag gaacgccgcg tcctgcggcg
      301 cgtccagggg cacctcgacg atatcgatcc ccgggcaggc cccgctcgag gagctgttgt
      361 agatgagcag ccgctcgttc gcgacgtcgg ggacgcctga ggccgtgtga gagccgcact
      421 cggtctccac cgacgcgacc agcacgggat cggagaggtc gctgatgtcg aagacgtgca
      481 gcccctcgaa gcccgccggc accggctggc catcacactg ttgcgaaccg gaggccggcg
      541 agttccacga ccggacgagg acgtcgtccc agacgaccac gtcgccctgg tccccggcgc
      601 agacggtgtg cgagacctgc ttcgggttcc ctggcgcgga gatgtctcgg atgttgaacc
      661 cgagccacgt cccctggaac gcgtgcttcc cccagaaggc gatgtcggtg tggatgtccg
      721 ggttcccgac ggccgggttg agcaagctcg ccggttccac gatgtgcccc atcggatgca
      781 gattgttcgt acgcggatcc gtctcgtgcc ctgcggtcgc cggcagcccc aagaggacca
      841 gcgatgccgc cgagatcacg agcacggacc atcggatgct tgaacgcacg ttgcctcctc
      901 tctcgatcgt gaacatggcc acgtttgcga ggcgcagggg gggtcccgtc cgcgctgcag
      961 ggatgcggag cagagatcgg gtcggatgcc tgccgggctc gcgtatgtcg gtgggctgag
     1021 agagcccatg gcctcgctcc ccccgaagcg atcgagaaga gccgtactct atcggcagta
     1081 cggggaaaac cacaaatccg tgttgaactg atggagagaa gcgggggcgg cttctgccgc
     1141 ccccgcttcg tccgccgctg tgggtcacgt gacctagagc gtgaactcct gggtctgcgg
     1201 gttcaggtgg tccaccttga tcgatcccgc aagcgacctg tggctgacct tgtacacggc
     1261 cagccccctg gtgatgtcgc tctcgtagat cgcgccgctg taccagtacg aggaccacgc
     1321 cccgccgatc tggcacatgc ctccgcagaa cgggcccggc ccgagcgact cgggatcggc
     1381 ccatgcgatc gcctcggcgt tcgccgggtc ggtgaagtcg accacccagg tgccggcctg
     1441 gtagtgacca cccaccgcca cgtagcggcc gctccggagc ggcacgatgt tgtagttgtg
     1501 gatggtgcag ttctcgtcgg cgccctgcgg ccgctccagc gtccactggc cgagcttcgc
     1561 cccggtctcc gaatcgtaga agaacatgct cttcttcacg tcggcgtccg ttgcctcaca
     1621 ctcggcggcg ccgccgccgc cgggctccca gcccagcacg atgacctcgc cgtcccacgt
     1681 gaaggccgcc gagtgccagt tgccgcccac accgacgccg ggctccgtga tcgtgtactg
     1741 gaagtccggg tcctccagcg agccggcgat gctgaacacg ttcgcggcgg cctgggaagc
     1801 gcacgccagc atcatcgtgt cccccaggat ggccccgcag tcgtgcgagg cgcccgcgtc
     1861 ggtcagcggc tcatcccgga tgtacgagag gtcgcccgtc gccaggtcga gctccagcac
     1921 gccgatgaag gggcacggcc ctccggacgt cgagttgtag atgaccagcc ggccgttctc
     1981 gaggtccggc acgccggtcg ccgtgtgcga cccgcatccg aacgcgtcgg cctccggcct
     2041 ggcgctgagc tccacggagt cgaccagcac ggggttgctc acgtcgctga tgtcgaagac
     2101 gtggaagccc tcgaagccct ctggcaccgg ctcaccgcca cactcaacgt cggggcgcgc
     2161 aggggcgttc caggagcgca cgacccagtt ctcccagacg atgacatccc cctggtcgcc
     2221 gtcgcagaag gcctcgccga cgagcttcgg gttacccggg gcggagatgt cgatgatccg
     2281 gaagccgttg taccggccct ggatcgccgt cttgccccag aacgcgagat ccgagttgac
     2341 gttgtcccgc tcccctgggt ccaggctgaa gaacccgggg ttctcgctgt gccccagcgc
     2401 gtggatggag ttggtctgcg gatacgtctt atgctcggcg cccgcgggga gcgccgtagc
     2461 tgccagagcc agcgtcgcga acacgatcac cgaccatcgg aaccttgggc gcatgcttcc
     2521 tccttcgtcg tcgtcgtgcc gaacgtccga tcgacatggg aatgcccgga gcagcatcac
     2581 ctccttcggc ttcgcaggag gacctcctga gccccgaacg taccaacggt gcgctcgtca
     2641 cgcaaacccg ggcaggggga aggcactcgg gggcgcgagc gggtcagccg accccgatct
     2701 cgtcgagcgc ccggagctcc ttccggagct cgttgacctc gtcgcggagc cgggccgcgt
     2761 actcgaactt cagctccttg gccgccaggt gcatctcgtc ctggaggctc tcgatcaggc
     2821 gcgtgagctc ctcgcgaggg agctccggcc gctcgggcgc ccgccggcgg cgcttggggg
     2881 ccgtctcggg ccgctcctcc accatcgtga ggaggatgtc cgagaccttc ttgcggatcg
     2941 tctgggggtc gatcccgtgc tcccggttgt gctccagctg gatcctgcgg cggcgctgcg
     3001 tctccgagat cgcggtgcgc atggagtcgg tgacctcgtc cgcgtacatc agcaccttgc
     3061 cctcgacgtt ccgcgccgcc cgcccgatcg tctggatcag tgaggtctcg ctgcggagga
     3121 acccctcctt gtcggcgtcg aggatcgcca cgagcgagac ctcgggcagg tcgaggccct
     3181 cgcgcaggag gttcaccccc acgagcacgt cgtactcccc caggcgaagg tcccggatga
     3241 tctggatccg ctcgatcgtg tcgatctcgg agtggaggta gcggacgcgg aggcccatct
     3301 cgatcaggta gtcggtgagg tcctcggcca tcttcttggt cagggtggtg acgaggacgc
     3361 gctggtcggc ctctgcccgg cgccgcacct tctccatcag gtcgtcgacc tgaccccgcg
     3421 tcggccggat ctcgacctcc gggtccacga gccccgtcgg ccgcacgatc tgctccacga
     3481 cccgcttcga gacctcgagc tcgtagtcgc tcggcgtggc cgacatgaac atcacctggt
     3541 tgacgcgctc cacgaactcg tcgaacttca gcggccggtt gtccacggcc gacgggaggc
     3601 ggaacccgtg ctccacgagg gtgcgcttgc ggctcatgtc gccctcgtac atcgcgtgga
     3661 gctggggcac ggtgacgtgc gactcgtcga tgatgcacag ccagtcgtcg gggaagtaat
     3721 cgatcagcgt gtacggggcc gtcccgggcg cccggccgtc gatgtgccgc gcgtagttct
     3781 cgatcccgga gcagaagccg acctcgcgca gcatctcgag atcgtggttg gtccgcatcc
     3841 gcagtctttg agcctcgagc agcttgctct tccgctccag ctgctcgagc cgaaccccga
     3901 gctcctcctc gatcccggcg atcgcccggt cgagccggtc gcgcgaggcc acgtagtggg
     3961 tcgcgggaaa gatcgtcacg tggtcgagct cgcggaccac ctcgcccgtg atgaggtcca
     4021 tctccaggat gcgctccacc tcgtcgcccc acagctcgac gcgcagcgcc gtctcctggt
     4081 acgaggggtg gatctcgagc gtgtcgccct gcacgcggaa cgtcccgcgg gccaggttcg
     4141 cctggttgcg ctggtactgc atgtccacga ggcgctgcat cgcggccgtg tggcccaccg
     4201 gccgcccccg caccacatgg aggatctgct gctcgtactc ctcgggcgag cccaggccgt
     4261 agatgcacga gacgctggcc acgatgatga cgtccttccg cgtgagcaga gccgcggtgg
     4321 ccgcgtgccg cagccggtcg atctcgtcgt tcaccgtggc gtccttctcg atgtagaggt
     4381 cggtctgcgg gacgtaggcc tccggctggt agtagtcgta gtaggagacg aagtactcca
     4441 cagcgctgtc ggggaagaac tcgcggaact cgttggcgag ctgggcggcg agcgacttgt
     4501 tcggcgagat cacgagggtg ggcttgccca gccgttcgat cacgttggca gcggtgaagg
     4561 tcttgccgga gcccgtgatg ccgagcagcg tgacgcggtc ctctccggcc gccagcccct
     4621 cggtcagttc gtcgatcgcc ttcggctggt ccccggccgg gcgcaggtcg gtctggacgc
     4681 ggaacgatga catggatgcg attctacggc ccgacgctca gagcgggcgc cgccccgaga
     4741 agcccgcgtc cacgtcgtgc cagtgcccga tccggtcctc gcccagtcgc cagcagaggt
     4801 agaccgtccg gccctcacgc tccgagggga agtccaccaa gccgccctcc gcgtcgcgga
     4861 gcacgatccc ccgcccggcc agatcctcca ccccccggcg gagcgcctcg atcgcccgcc
     4921 agtagtcggt gccctcccgc ccaccgccgt tccgccgggc cacgccgcgc acccgctccc
     4981 cggacgccag cacccgtcgg cgggcctcgc gcagccgggg cagcagctcc cgcagccggg
     5041 gcagctccgc ctcggcctcc tcgagcgtga accgccgctc ctgcatcaga cccgcagccc
     5101 gacggcctcc gggaggtcct ccagcgaggt gatgcgcacg ccgtcggcct ccgggtagag
     5161 gccgcgccgg tcgatcagca cggggcgcat ccccacctcc ccggccgcct cgacgtcgaa
     5221 gtaggggtgg tccccgacgt agacggactc ctcggccgcc acccccatcc ggtccagggc
     5281 gatccggaag atggccgggt ccggcttctc caccccctcg atgcccgaca cgacccgcac
     5341 ggggaagaag cgcgtgaccg ccagcttctc caggaggccc tccagccact cctcgaagtt
     5401 ggagatgacc cccatcggat agccgccggc ctcgaggcgc tccagcagcg ggagggcgtc
     5461 ggggtggagc cggtagctct cgaggtcgct gaaccgcgcg tacagcgcgt cggtgagccg
     5521 tccgtcaggg tcctcgatgc ccgtgtcgcc cagcagcccc aggtacatcg agaaccagaa
     5581 gcgtcgggac cggtcggccg aggtggacca gagcgcgtgc tcgtcgctcc ggagcgtctc
     5641 cgtgaagcgc tgggaagccc cgacggccag ctcccgcacc atctcggggt cgacctcgtg
     5701 cccgtggtcc ctcagcacct cggcgaacag ctctgggaac gaggggtggg ggtggacgag
     5761 ggtctccccc gcatcgaaga acacggcttt ggtctccatc ggcgcgatga tagccgccct
     5821 cagccccgag actcgcgccg caggcgatcc cacagggcat ccacctgccg ctccagatcc
     5881 cgcaggtcgc cttcgttgcg gaggatcacg tcggccttgg cctcgcgctc ctcctgcgag
     5941 gcctgggccg cgatccgggc ctccgcctcg acccggtcca tcccccgggc cacgacgcgc
     6001 tccacctgcg tctccggggc caccgccacc accacgagca ggtccatggc ggtgtccacg
     6061 ccggtctcca cgatgagcgg cgcgtcgaac accacgacgt cgcccgtgtc ccgccggcgc
     6121 tccacctctt cggccagcat ccggaagacc tcggggtggg tgatggactc gagcgcgcgc
     6181 ctggcctccg ggtctccgaa caccacgtcg gccagggcgc ggcggtcgat cgagccgtcg
     6241 ggggcctcga cctcgtcccc gaagacctcc agcaccttgc ggtggccccg ggtccccggc
     6301 tcgatcgacc gccgcgccag ctcgtccgcg tccacgatca cggccccccg ctccgccagc
     6361 atgcgggcca cggtggactt gccggctccg aggccgcccg tgaggccgac caggagcatg
     6421 gggaggggcc tactggcggc cttcgcggcg gcggaggtcg gccacgatgt cctcgaggga
     6481 gacgtcctcc tcgtcctggc ccgcgtcctc cgcggccggg ggctcctcgg gggtgatctc
     6541 cggctcggcc tccggctcct ccggctcggc ctcggcctgg ggagcggcct cggcccgggg
     6601 ctcagcctcg ggctcgggct gcagtgcctc ctcgaccggc tcctcgggct ccggctcggc
     6661 ctccgccggg gcctcgggca cgacctcgtc gggctccggc tcgaactccg gcgtgatgat
     6721 ggcgtcgccc tcgacgcgcc gcatcgagag cgagatccgc cgccgggaga cgtccacgtc
     6781 cacgaccttc acccggacgc ggtcgccgac gttcacgacc tcgtcggccg actccacgtg
     6841 cgcgtcggac agctcgctga tgtggaccag cccctcgatg tcgtcggcca cacgcacgaa
     6901 ggcgccgaac ggcaccagct tcgtgacctc gccctcgatc acctcgccgg cgttgtactt
     6961 ccgctcgaac tccttccagg ggtcttcctg ggtggccttc agcgagagcg agacccgctc
     7021 ccgctccagg ttgacgtcga ggacctccac ctcgacctcc tcgcccacgg agacgacctc
     7081 gccgggatgc tcgacgtggc gccaggagag ctcggagacg tgcaccaggc cgtccacccc
     7141 gcccaggtcc acgaaggcgc cgaagttcac gatcgaggag accttccccg tccgccgctc
     7201 ccccggctgg agcgactcga gcaccttctt gcggctctcg ctctgcgact cctccaggaa
     7261 cgcccgccgc gacaggacca cgttgttccg gttgcggtcg agctcgatga tcttgcactc
     7321 gagaacctgc cccacgaacg gatgcaggtc gcgtacccga cggagatcca cgagcgaggc
     7381 cggcaggaac ccgcggaggc cgatgtcgag gatgaggccg cccttgacca cctcgatcac
     7441 ggggccctcg atcgtgccgc cggactccat gacctcctcg atgcggcccc aggcccgctc
     7501 gtactgcgca cgcttcttgg agaggatgag gcgcccctcc ttgtcctcct tctggaggac
     7561 gagggcctcg agctcgtcgc cgatcgagac ctcctcggag ggatccacgt cgtgccggat
     7621 cgagagctcc ttggtgggga tcaccccctc ggacttgtag ccgatgtcca ggagcacctc
     7681 gtcggggtcg atccggacca cctgcccgga tacgaggtcg ccgtcgttca ggtccttgac
     7741 ggattcctcg atggccttgg ccagctcctc gggcgtcagg tcgtcctggt ccggggcgac
     7801 gccatccctc tggttcgtct cttcgatctc cgatcagctc ctctctgccc ctgcgcagcg
     7861 gcgctcacgc ggaaccggat cgaggtgagg gatccgggca cgcagactgc gccccgaagg
     7921 gcccgaacat cttaggggac ctgcgaagat gccgtcaatc gcgtcagtgg ccctccgggg
     7981 tcgcgtcggc ccacgtgggc ccgctcccga cgtccacccg cagcggcacc gagagctcgt
     8041 aggccgagga catctcctcg cggacggctg cggccacctc ctccaccgac gcctcctcca
     8101 cctccaggac cagctcgtcg tggacggtga gtaccatcgt tgccggcagg tcgacgaccc
     8161 gctggtccac gcggatcatc gccaacttga tgatgtcggc ggcggagccc tggatcgggg
     8221 cgttgagcgc catgcggcgc cccaggtcgc gcacccgcgg gttcggcgac tgcagctcgg
     8281 ggaggtagcg gcgccggccc aggatcgtct gggtgaatcc ctccgccgcc gccatcgtca
     8341 cctggtgatc gaggaactcc cgcacccgcg ggaacccctc gaagtaggcg tcgatgaact
     8401 ccttggcctc gtcggggggg atccccaggc gggtggccag cccgtaggcg ttcatgccgt
     8461 aggccagccc gtagttgacg accttggctc ggcggcgcag ctccgagtcg acctgctcgg
     8521 gcgggaggcc gaacaccttc gcggcggtgg ccgagtggat gtcggcgtcc tcggcgaacg
     8581 ccgccccgag cgcctcgtcg cccgagaggt gcgccaggac cctcagctcg atctgggagt
     8641 agtcggccac gagcaggacc cggccctcct cggcgatgaa ggccttgcgg atctcccgcc
     8701 cctgctcccc gcgcacgggg atgttctgga ggttcgggtt gagcgaggac agccggccgg
     8761 tcgccgccgc cacctggctg aaggtggtgt ggacgcgacc cgtggccggg ctcacgagcg
     8821 gcgggagcgc gtcgaggtag gtggacttca gcttggccag ctcccgatac gagagcagcg
     8881 cctccacgat cgggtgctgg tgccgcagct tctccagcgt ggcggcgtcg gtcgagtagc
     8941 cggtcttcgt ccgtttcccc ggggtcagcc cgagcttctc gtagaggatc acccggagct
     9001 ggggcggcga gttgaggttg aactcctcgc cggcgtgctc gtagagctcg gactcgagcg
     9061 tccgcatccg gtcgccgagg gactcggaca tctcctggag gtacgggacg tcgatcttca
     9121 ccccggcggc ctgcatccgg gccagcaccg tggccagggg caactcgacg tcgcggagca
     9181 ggtcggcggc gccctgccgc tcgagccgct cggccatcag gggggccagg atcgccaccg
     9241 ccgcggcctc cgccgccagc ggcccccggt catcctcctg cagggcgagc tggccgtcct
     9301 cctcgggggt ctcggtcgcc tcccccacca ggtcgacgcc gaggtaccgc tcggagaggg
     9361 ccccgatcgg gtaggaggcc tcggcgggat ccaggaggta gccggccagc atcgtgtcga
     9421 cggcgatgcc ggccgcctcc acgccgatcg gcagcagcgc cgcctggagc tccttggcgt
     9481 cgtgcaccca gatcggtcgg tcgggggcct cgagccacgc gcggaggggc tcggggagcg
     9541 agccgtcgag gggtgcgtgc accgcccgtc catcccccgc cgagaccgcg atcgccgacg
     9601 ggccgccttc cgtcgcgcgg agcgccaccg cggccggccg cggccgctcc agcacggtgg
     9661 ccaccgcgtc cgcgtcgccc tcctcgatcc tgagctcgac gtcggccgcg gccggccgcg
     9721 gctcgcccac ctcctggagg cgctccagga gggagcggaa ctccagggcg ttaaagagcg
     9781 ttcggatctg gtcctggtcc cactccccca cccggcactc ctgaggctcg atgtcgaggt
     9841 ccagatccgt gacgagccgc acgagctccc ggttccggag gaggtcgtcg cccgcctcct
     9901 cgatccccgc ccggagcttc ggggtgaggt ccgcggcgtg ctccaggacc tcctcgaccg
     9961 tcccgtgctc ggtgatcagc ttcgcggccg tcttgtcccc cacccccggc acgccgggga
    10021 tgttgtcgga cgtatcgccc ttcaacgcca cgagctccac gtacttgtcc gggggcacgc
    10081 cgaaccgctc ggtcaccgcc ttctcgtcgt agtcgacgat gtccgagatc cccttgcggt
    10141 tgaacttcac gcggatcccg ggccgcacga gctggaagaa gtcgcggtcg gccgtgacga
    10201 tcacgacctc gagccccttc tccgcggccc gctcggcgat cgtggcgatc gcgtcgtcgg
    10261 cctcgtggcc ctcgacctcg atgatcggga tccgcagggt ctccagcacc tcgcggatca
    10321 gggggagctg ctggcggaac tcgtcggggg tctccgctcg ccccgccttg tactcggcgt
    10381 actgctcgag gcggaccgtc ggcctcccct tgtcgaacgc caccgccacg tactcgggat
    10441 gctcctccgc caggagcttg atcagcatcg aggtgaagcc gtagacggcg ttcgtcacct
    10501 gccccgtggt ggtggccagg tcgggtggga gcgcgaagaa ggctcggtag gagaggctgt
    10561 gcccgtccag caggaagagt cgctcggtcc ccacgcgtgc cgattgtaga ctctcctcca
    10621 tgacggccgg gggctcccat caggaggact acctcgaggc gatctacgag atggccgagg
    10681 aggggatccc cacccagcag gcccggatcg ccgaatggct gggcatcacg ccggcctcgg
    10741 tctcggaggc cgtgaagcgt ctcgtccagc gggagctggt ggaggtcggc gccgaccgca
    10801 ggctcgtctt cacggccgac gggcgggccc tggccgagac cctggtgcgc cgtcaccggc
    10861 tcgccgaacg gttcctcgtc gaggtcgtcg gtctcccctg gtacctgtcc cacgaggagg
    10921 cgaccgtctg gggccgggcg atctcgtccg ccgtcgagga gcggttcgtg gccatgctcg
    10981 gcgaccccgc cacgtgcgtc cacggcaacc cgatccccgg gacgaggcgg ccggtggacc
    11041 agcgggacct ccgtccgctc ccggcgctgc ccgaaggggc ctcggggcgg ctggagcggc
    11101 tcaccgagga cctggagctc gacctggacg tgatgcggtt cctgcaggac agcggcctga
    11161 tgcccggggc cggcctcacc gtggcctcgg tgggccccga cggcacccgg accctcgagg
    11221 tggacggaga tcgcgtcgcc ctcgggccgg agatcgccga caacctctgg gtccggacct
    11281 cctgagcccg agcgggctcg gttagactcc cgccgatgag cgagaaccgc acgcctcccg
    11341 ggttcacgcg ggagcaggcc ctggccacgg ccggggagat cgggaggggc accttcctgg
    11401 agcgcatcga gctggagtgg ctcgaggtcg ggctggaccg gctcgtggca cggatccccg
    11461 tggccggcaa cacccagccc tacggcatcc tccacggagg ggccacggcg gcgctctgcg
    11521 agacggtggg atccttcggg acttcgatcc acgtcgggct cgatcggcgc cccgtggggg
    11581 tccagctgtc cgtgaaccat ctccgcgcgg tccgcgaggg gcacgtcacc gcgacggggg
    11641 tgcccctcca cgtcggccgc acgatcgctc tgtgggagat acgggtcgag gacgacgggg
    11701 ggcggctcgt cgccgtgggc acgctgacgc tggtgatccg ggacgccgtt cccggatgat
    11761 cggcacgctc atcaacgtgg ccacggtcct ggccgggacc ctggccggga ccctcttcgg
    11821 agaccgcctc cccgagcgga tccgcgagac ggtcctccac tcgctcggcc tgatcaccct
    11881 gctcgtcggc ctggatgccg ggctgcacac cttccgggcc cccctatcgg agacgacgcg
    11941 cgccagcgcg atcctcgtca tgggagccgt cctcgtcggg gggatcctgg gcgagctcct
    12001 ccacatcgag cggggcctca acggggtcgg cgaccgcctc aaggccaggt tcggcggggg
    12061 ccaggccagg ttcaccgagg gcttcgtcgt ggcctcgctc gtgttctgcg tgggacccct
    12121 gacgatcctg ggggccatcc aggacgggac gacgggcgac tttcagctcc tggcgatcaa
    12181 ggccaccctc gacgggttcg cggccgtcgc gttcgcctcg gccctgggct ggggcgtggg
    12241 gttctcgacc atcaccgtcc tcgtctacca gggcgcgctg tcgctcacgg ccgcgaccgc
    12301 ggcctcgatc ttcaccgacg tggtcgtgga ggcgatgacc gcggccgggg gcgtcatcat
    12361 cgtcggcatc ggcctccgcc tcctcgagat ccgcgaggtg cgggtgggga acctcctgcc
    12421 ggcgctcgtc ctggcccccg tcgcgttcgc cgtcgtggag cgcgtcggct gaccaccccg
    12481 ttcctagggt tttgtcactc ggatccgctc ggcggcccag atttttccgg cggggcgacc
    12541 cgcccgcgac ctgggccgtt gcccgagagc cgttgcggga cagcctatct ccgttccttg
    12601 actccgcctt cgccgccctg ctagtttccc cctcggtccc gagcggccgg agggacgacc
    12661 gactcggggg ctccggagga gcggagcagg ccaccggacg ggccccggcc cggacggcga
    12721 gcccagaccg accttccgga cgcccgccga gacgcccggc cccggccggg gagtgccgag
    12781 ggttgctcgg gaccttcgtt ttggcgggct cgaggtgccg agtagacaac gctcgcgtat
    12841 ggggaaggcg tgagcggctc caaaggcacc tcgagcccgc cgagcgtacg gcccctcagg
    12901 cgatgatccg cgagaggaac gagcgggtcc gctcgtgctg gggctcggcg aacatctcct
    12961 ggggtcgccc ctcctccagg atccggccct cgtcgatgaa gaccaaccgg ttgcccacct
    13021 ctcgggcgaa ccccatctcg tgcgacacga tcaccatcgt catgccctcg gaggcaagcc
    13081 gctgcatcac gtcgaggacc tccttgacga gctcggggtc gagcgccgac gtgacctcgt
    13141 cgaacagcat cagcttggga cgcatcgcca gcgcccgcgc gatcgccacc cgctgctgct
    13201 gacccccgga gagctgcgat gggtaggagt cggccttctc cgccaacccc acgtggtcga
    13261 gctgctcccg ggcgcgctcc tcggccgccg agtcgtccag cctcaggacc ttccgcaggg
    13321 cgatcgtcac gttcctcagg gccgtcatgt gcgggaagag gttgaacgac tggaacacga
    13381 tcccgatctc ggtgcgaacg tcgtcgacgt cggcacggac gtcggtgatg tccttcccgt
    13441 cccagatcac gctgccctcc tggggctcct ccagcaggtt gatcgtgcgc aggagggtgg
    13501 acttccccga gcccgagggg ccgatcagca cgagcacgtc ctgagggttc accgcgaggt
    13561 cgacgtcgcg caggacgtgg ttctcgccga accacttgtt gatgccctcg aggcgcacca
    13621 gcgtgccgtc ggggttcacg cccccacccc cacgagcccg ctgcggagct tccgctccag
    13681 ccagctcacg gcgcggatca tgggcagcgt cacgatcatg tagccggccg cagccgcgag
    13741 ccacggcgtg gcgttgaagg tctccgcgta gaggtcgcgg gcccacgtca ggagctcacg
    13801 ctggtccgcg gtaagcccga gcagggtcac gagcgaggtg tccttgatca ggatgatgaa
    13861 ctcgttcgtg agcggcggga tcacccgtcg gaacgcctgc ggtaagatcg cgtagcgcat
    13921 cgcctggaag tacgtcatgc ccagcgatcg ggctgcctcc atctgccccc gctcgatcga
    13981 ctggattccg gcgcggaaca cctcggccgt gtacgcgccc gcgttgaggg cgaagatgaa
    14041 cacggcggcc tggaacacgg tgaccgtctg agccagcccg aggcccagga cgaagcccag
    14101 gtagaagaac gagagctgcc agaggagggg cgtcccccgg aagaagttga tgtaggcccg
    14161 ggccggggcc cggaccacgg cgtgctgtga gagcgccatc atcgccagga tcaagccgat
    14221 cacgatcccc cccacctctc cgaagaacgc cagcaggatc gtgttcttga tcgccgtgat
    14281 gaagtcgggc aggaacccct cgagcacctc gaagcgcagg ttctggcgga cgaggaaggc
    14341 gaagttctcc cccgagcgga ccaggagcag gaggccggcg gccaccaccg cctggacgcc
    14401 cagcgcggcc cccgagatcg ccgccttcct ggcgggcttg gtcggcatcc ggcggtagat
    14461 gccgaagccc accgccgacg ccacaagccc cgcggccacg gccgcgaaca gcagcgtggt
    14521 cgcggggccg ctctggatgc tctccaggat cgtcgcgtcg gggatccatc cccgggccgt
    14581 gatgacgagc gggatgccga gcagcacgat gacgagggac gtggccccga cgatcagcac
    14641 ggccagcgag agcgtgttct gctccgcagc tccatgtgcc gcgtgacgtc gccgatgagc
    14701 gaaccatccg cgcaaccgct ctccccggga cttcatcgcg ccctccctcg tcctcggagg
    14761 cggtgggggg g
//
LOCUS       DASUTW010000115         2224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1329691, whole genome shotgun sequence.
ACCESSION   DASUTW010000115 DASUTW010000000
VERSION     DASUTW010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329691"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..238)
                     /locus_tag="VF058_03740"
     CDS             complement(5..238)
                     /locus_tag="VF058_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207450.1"
                     /translation="MGEDRHSHEATVHAHEHVHMTHYARPDEEATHLEATHEHEHNHP
                     ALEHVHEAHEDAEKEHLREAHIHDHASPAQSPA"
     gene            367..633
                     /locus_tag="VF058_03745"
     CDS             367..633
                     /locus_tag="VF058_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207451.1"
                     /translation="MGFKTGMIIGFGVGYVLGTKAGRERYEELRQSWQDLMGNPRVQE
                     AVERGKGLVGTATREGLTAVQGGVEKAAGAVKERLDKDEDLGTG"
     gene            855..>2224
                     /locus_tag="VF058_03750"
     CDS             855..>2224
                     /locus_tag="VF058_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX6207452.1"
                     /translation="MFERFTDRARRVVVLAQEEARLLNHNYIGTEHILLGLIHEGEGV
                     AAKALESLGISLEAVRQQVEDIIGQGQSAPTGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAKVLQKLGADLNRVRQQVIQLLSGYTGKEATAGAPGEPSP
                     QGSMVLDQFGRNLTQLAREVKLDPVIGREKEIERVMQVLSRRTKNNPVLIGDPGVGKT
                     AVVEGLAQAITKGEVPETIKDKQIYTLDLGALVAGSRYRGDFEERLKKVLKEIKTRGD
                     IILFIDELHTLVGAGAAEGAIDAASILKPMLARGELQTIGATTRDEYRKHLEKDAALE
                     RRFQPIDVSEPSVAHTIDILKGLRDRYEAHHRVSFTDDALVAAANLSDRYISDRFLPD
                     KAIDLIDEAGSRMRIRRMTAPPDVREFDEKIADVRMRKESSIDAQDFETAARLRDEER
                     QLAEQRS"
BASE COUNT          376 a          714 c          824 g          310 t
ORIGIN      
        1 gccatcacgc cggggactgt gccggggagg cgtggtcgtg gatgtgcgcc tcccgcaggt
       61 gctccttctc cgcgtcctcg tgcgcctcgt ggacgtgctc gagcgcgggg tggttgtgct
      121 cgtgctcgtg ggtggcctcg aggtgcgtgg cctcctcgtc cggccgagcg tagtgggtca
      181 tgtgcacgtg ctcgtgggcg tgcaccgtgg cctcgtggct gtggcgatcc tcgcccatct
      241 cgactcctcc ttcctggccg gagtcctccc ccgcatgagc ctaaccgaac ctgctagggt
      301 cccgtgaggt cggctcgttt gtggccatga tcgcgaggga atggtgcggg cgacaaggag
      361 ggcgcgatgg gattcaagac cggcatgatc atcgggttcg gcgtcggcta cgtgctcggc
      421 accaaggccg ggcgcgagcg atacgaggag ctccgccagt cctggcagga cctcatgggg
      481 aacccccggg tccaggaggc cgtcgaacgg ggaaaagggc tggtggggac cgcgactcgt
      541 gaggggctca ccgcggtcca ggggggcgtc gagaaggccg ccggcgcggt gaaggagcgc
      601 ctggacaagg acgaggacct gggcaccggc tgagggaaca gcccgcgatc ggcccgcgtt
      661 gacctacggt gtcggcctcg gcgtgccgat ggaacgtcca ggcaacgggg catgccggag
      721 gccgggggta taatcggcac catcgggcgc tccgaagggt gggagcgccc gagacgcctc
      781 gggggtagga cggagtccac ggatccccga ggggacccga cagggccggg atgaccggcc
      841 cagagggaga acgaatgttt gagcggttca ccgacagggc ccgcagggtc gtcgtcctgg
      901 cccaggagga agcgcggctc ctgaaccaca actacatcgg gaccgagcac atcctcctcg
      961 gcctgatcca cgagggtgag ggcgtggccg ccaaggcgct cgagtcgctg gggatctccc
     1021 tggaggccgt ccgccagcag gtcgaggaca tcatcggcca ggggcagtcg gcgccgacgg
     1081 gccacatccc gttcacgccg cgggccaaga aggtgctgga gctctcgctg cgcgaggcgc
     1141 tccagctggg ccacaactac atcgggaccg agcacatcct cctgggcctg atccgggagg
     1201 gagagggcgt ggccgccaag gtgctccaga agctcggggc ggacctgaac cgcgtccgcc
     1261 agcaggtgat ccagctcctg tccggctaca cgggcaagga ggccaccgcc ggcgcgcccg
     1321 gggagccgtc gccgcagggg tcgatggtcc tcgaccagtt cggccggaac ctcacccagc
     1381 tggctcgcga ggtcaagctc gacccggtca tcgggcgcga gaaggagatc gagcgcgtga
     1441 tgcaggtcct gtcccggcgc accaagaaca acccggtgct gatcggggac ccgggcgtcg
     1501 gcaagacggc cgtggtcgaa ggactggccc aggcgatcac gaagggcgag gtgcccgaga
     1561 cgatcaagga caagcagatc tacacgctcg acctcggggc gctcgtggcg gggtcgcggt
     1621 accgtggcga cttcgaggag cgcctgaaga aggtgctgaa ggagatcaag acccgcggcg
     1681 acatcatctt gttcatcgat gagctgcaca cgctggtggg cgccggcgcg gccgagggcg
     1741 cgatcgacgc ggcctcgatc ctcaagccga tgctggcccg cggcgagctc cagacgatcg
     1801 gcgccacgac ccgggacgag taccgcaagc acttggagaa ggacgccgcc ctcgagcgcc
     1861 ggttccagcc gatcgacgtg agcgagccga gcgtcgctca cacgatcgac atcctcaagg
     1921 ggctgcgtga ccgctacgag gcgcaccacc gcgtgtcgtt caccgacgac gccctcgtgg
     1981 ccgcggcgaa cctctccgac cgctacatct cggaccggtt cctgccggac aaggcgatcg
     2041 atctgatcga cgaggccggc tcgaggatgc ggatccgtcg gatgaccgcg cctcccgacg
     2101 tcagggagtt cgacgagaag atcgccgacg tgcggatgcg caaggagtcc tcgatcgacg
     2161 cgcaggactt cgagacggcg gcgaggctcc gagacgagga gcgtcagctc gccgagcagc
     2221 gctc
//
LOCUS       DASUTW010000116         5942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_424248, whole genome shotgun sequence.
ACCESSION   DASUTW010000116 DASUTW010000000
VERSION     DASUTW010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424248"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VF058_03755"
     CDS             complement(<1..372)
                     /locus_tag="VF058_03755"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HEX6207453.1"
                     /translation="MSRLIVAADVPSVAAAEELARSLAGTVGMVKVGLELYAAEGPEA
                     VRRLRGHLPVFLDLKLHDIPTTVGRASRVVGRLGAAMLTVHALGGPEMIAAAVRGTAE
                     GAAEEGMEPPEVIAVTVLSSLA"
     gene            complement(347..1312)
                     /locus_tag="VF058_03760"
     CDS             complement(347..1312)
                     /locus_tag="VF058_03760"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX6207454.1"
                     /translation="MARARVSLGVDLGGVQLPTPVLGASGTFGSGRELAPVLDLSKVG
                     GIVTKSVTLRPVKGLPTPRMVETPSGMLNAIGLQNPGIDEFLEKEGPFIRGVGVPVIL
                     SIAGKSVEEFVQVAMRAQHVPGVVALEANISCPNVERRNQVFACHPDAAAEVVGAVAR
                     LSRLPVFAKLTPDVTDIVEVADACVRAGAHGISLINTLLAMAIDVETFRPRLGGVTGG
                     LSGPAIRPVAVRAVYQVARALPEVPIIGMGGIRSARDAVEFLLAGAWAVAVGTASFWN
                     PESVVDVGRGIGELLVRRGLRSPADLRARVVVEEEEAGEPAHRGG"
     gene            complement(1294..2190)
                     /locus_tag="VF058_03765"
     CDS             complement(1294..2190)
                     /locus_tag="VF058_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEX6207455.1"
                     /translation="MIRKKCEVLSSRRAGVYHTLTLVAPEIAEVARPGQFVQIAVPEG
                     REFLLRRPFSIHEASRRGGWAGTLELAFLGTGPGTTWLTQVQPHDFLDVIGPLGKPFP
                     YPRQLTNCLLVGGGYGAAPLYFLAKELNARQKRVDMIIGARDQDRVFKQVEAKRLSVS
                     IGITTEDGSLGDRGRVTDILPGMVSRTGAEVVYACGPNAMLRAVAAYCADHGIPCQVA
                     VEELMACGLGVCWTCTVPVIRRDGSGWDNLRTCVEGPVFNGARVWWEKWLREPSVPTQ
                     TPPHGFRMEEAGVGGEPWPEHE"
     gene            2436..2642
                     /locus_tag="VF058_03770"
     CDS             2436..2642
                     /locus_tag="VF058_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207456.1"
                     /translation="MLPALPAWVALDDVDPAGNPGFEIDASMTASHLTVTVEQAAGQE
                     DPTNAPPIGRPGPGNDVILGCERP"
     gene            complement(2696..3994)
                     /locus_tag="VF058_03775"
     CDS             complement(2696..3994)
                     /locus_tag="VF058_03775"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003959743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX6207457.1"
                     /translation="MAKILFRGGRVVDPASGLDGPADVLVADTLVEEVGPELSPSGAE
                     VVDCDGLVVAPGLVDLHTHLREPGREDAETIETGSRAAALGGYTAVVAMPNTDPVADQ
                     LAVVLEVAARGEKAGMVEVIPASAITKGLEGESLVEMGELAAAGVRLFTDDHRSVQSA
                     RTMRLALEYAKAFDVVICQHAQDDTLAEEAQMHEGHYSALLGLRGFPREGEEVTVARD
                     ILLARLTGGRLHVTHTSSAGSVELIRRARAEGVRVTADATPHHLALTDEALVTYDTRF
                     KVNPPIRSEEDRDGLRQGLADGTIDAVATDHAPHAVEDKEVEFDHARPGTTGLETALA
                     VVVTELVEPGLLDLPSAIRAMSTEPARMLGLHEQGGPLEPGQAANLVVFDPAEAWTVG
                     ERPFATKGRNSAFLGHSLRGRVRHTLLRGAFTVREGEAAG"
     gene            complement(3994..4923)
                     /locus_tag="VF058_03780"
     CDS             complement(3994..4923)
                     /locus_tag="VF058_03780"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014446324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX6207458.1"
                     /translation="MRDLLSITQLSAEDVTRILDTADSFREVGTRVIKKVPALRGRTV
                     VNLFYENSTRTRISFELAAKRLSADVINFSTSGSSVSKGESLKDTALTLQAMGADAIV
                     IRHSSSGAPYTLSRWVDARVLNAGDGTHEHPTQALLDLYTIREHFPSFEGLKVAIVGD
                     VRHSRVARSGVLALTKMGADVTLVGPPTLIPPEAPSWGAEVSYELDPVLPKLDVCYML
                     RVQRERQRQQFFPTVREYSQRYGLSAERVAALPDVALIMHPGPMNRGVEIASDVADLP
                     RSLIVDQVTNGIAVRMALLYLIIGVEGEQKEAS"
     gene            complement(4923..5498)
                     /gene="pyrR"
                     /locus_tag="VF058_03785"
     CDS             complement(4923..5498)
                     /gene="pyrR"
                     /locus_tag="VF058_03785"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX6207459.1"
                     /translation="MGRSRVRERVVAFTASGVVLDADGVRRALTRIAHEILERNAGAA
                     DLVLVGIAARGDDLARRIAEELRRIEDVDVPVGVLDISFYRDDIGLRAEAPEVHETRI
                     DFDVTGRTVVLVDDVLFTGRTIRAAMDALVDFGRPRAIQLAVLVDRGHRELPIRADFV
                     GKNIPTRTEDQVRVLTSETDGADAVEVGEET"
     gene            5643..5867
                     /locus_tag="VF058_03790"
     CDS             5643..5867
                     /locus_tag="VF058_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006340664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX6207460.1"
                     /translation="METVAVWTPGLGELIIILVVVLLLFGAKRLPEMARSLGRSTKEF
                     KKGLDEEAEEEELREADESRPAGDPPTPSR"
BASE COUNT          862 a         2197 c         2076 g          807 t
ORIGIN      
        1 cgccaggctg gacagcaccg tgaccgcgat cacctcgggg ggctccatcc cctcctcggc
       61 cgccccctcg gcggtcccgc gcacggccgc ggcaatcatc tcggggccgc ccagcgcgtg
      121 caccgtcagc atcgcggcgc cgagccgtcc cacgacgcgg gaggcccgcc ccacggtcgt
      181 ggggatgtcg tgcagcttca ggtcgaggaa gacggggagg tgcccccgga ggcggcggac
      241 ggcctcgggg ccctccgccg cgtagagctc gaggcccacc ttgaccatgc ccacggtgcc
      301 ggccagggag cgggccagct cctccgcggc ggccaccgat gggacgtcag ccgccacgat
      361 gagccggctc accggcctcc tcttcctcca ccaccacccg agcccgcaga tccgccgggc
      421 tccgcagccc gcggcggacc aggagctccc cgatcccgcg gcccacatcg accacggact
      481 cggggttcca gaagctcgcc gtccccaccg ccacggccca ggcgcccgcg agcaggaact
      541 cgaccgcgtc ccgggcgctg cggatccctc ccatcccgat gatggggacc tcggggagcg
      601 cccgcgccac ctgatagacg gcgcgcacgg ccaccggccg gatcgcgggg cccgacagcc
      661 cgcccgtcac cccgcccagc ctggggcgga acgtctccac gtcgatcgcc atcgccagca
      721 gcgtgttgat gagcgagatc ccgtgggcgc cggcgcggac gcaggcgtcg gccacctcga
      781 cgatgtcggt gacgtcgggc gtgagcttgg cgaagaccgg cagccgagag agcctggcca
      841 ccgcccccac gacctcggcc gccgcgtccg ggtggcaggc gaagacctgg ttgcgccgct
      901 cgacgttggg gcaggagatg ttggcctcca gcgccacgac cccggggacg tgctgggcgc
      961 gcatcgccac ctggacgaac tcctccaccg acttccccgc gatcgagagg atgaccggga
     1021 cgcccacccc acggatgaag ggtccctcct tctcgaggaa ctcgtcgatg ccggggttct
     1081 ggaggccgat cgcgttcagc atgcccgacg gggtctcgac catgcggggg gtggggagcc
     1141 ccttcaccgg gcggagcgtg acgctcttcg tgacgatccc cccgaccttg gagagatcga
     1201 ggacgggcgc gagctcgcgg ccggacccga acgtcccgga ggccccgagc acgggggtgg
     1261 ggagctgcac gccccccaga tcgaccccga ggctcactcg tgctcgggcc acggctcacc
     1321 tcccactccc gcctcctcca tgcggaaccc gtggggaggg gtctgcgtgg ggacagaggg
     1381 ctcccggagc cacttctccc accagacgcg cgcaccgttg aagaccgggc cctccacgca
     1441 cgtccggagg ttgtcccagc cggacccatc ccggcggatc accgggaccg tgcaggtcca
     1501 gcagacgccc aggccgcacg ccatcagctc ctccacggcc acctggcacg ggatcccgtg
     1561 gtcggcgcag tacgccgcca ccgcccggag catcgcgttc gggccgcacg cgtacaccac
     1621 ctcggcgccc gttcgcgaca ccatcccggg gaggatgtcg gtgacgcggc ctcggtcgcc
     1681 gaggctgccg tcctcggtcg tgatcccgat cgacaccgag agacgcttcg cctcgacctg
     1741 cttgaagacg cggtcctggt cgcgggcccc gatgatcatg tccacgcgct tctgacgggc
     1801 gttcagctcc ttcgccagga agtagagggg ggccgccccg tagcccccac cgaccaggag
     1861 gcagttcgtc agctgtcgcg ggtacgggaa gggcttcccg agcggcccga tcacgtcgag
     1921 gaagtcgtgc ggctggacct gcgtcagcca ggtggtgccc gggcccgtcc ccaggaaggc
     1981 gagctccagg gtgcctgccc aacctccacg cctggacgcc tcgtggatcg agaagggacg
     2041 gcgcagcagg aactcgcggc cctcggggac ggcgatctgc acgaactggc cggggcgtgc
     2101 cacctcggcg atctccggcg ccaccagggt cagcgtgtgg tacacgccag cacgccgcga
     2161 gctgaggact tcgcacttct tgcggatcac ggcaccgagc ctactccccg tccgaggtgg
     2221 gcatggtact cctgctcgct cgggacctcc tgcgcaagcc cgatcgagac gtcgccgtcc
     2281 gccccgagga gcgatgagct gctgcgtcga gctgaatcga cgcgacatgg atacgttgag
     2341 gccggtcggg gacggagagg cggaccacgc gcgggatctc gaggagagga cgggccctca
     2401 gggccctgag ggcggcgtgc ggaacgatcg ggctcatgct gcccgccctg ccggcctggg
     2461 tcgcgctcga cgacgtcgac ccggcgggca atccagggtt cgagatcgac gcctcgatga
     2521 cggcgtccca cctcaccgtg acggtggagc aggccgcggg gcaggaagac cccacgaacg
     2581 cccctcccat cggccggccg gggccgggca acgacgtgat cctgggctgc gagcggccct
     2641 agctctcaat ggcgcgagga cggcggtagg cctcgggagg ccgccggctg aggcttcagc
     2701 cggcggcctc cccctcgcgc accgtgaacg cgccccgcag caaggtgtgt cggacgcgcc
     2761 cgcggaggct gtggcccagg aaggccgagt tgcggccctt cgtcgcgaac ggccgctccc
     2821 ccacggtcca ggcctcggcc gggtcgaaga cgacgaggtt ggccgcctgc cccggctcga
     2881 ggggcccgcc ctgctcgtgg aggcccagca tccgcgccgg ctccgtggac atcgcccgga
     2941 tcgcgctcgg caggtcgagc aggccgggct ccacgagctc ggtaaccacg accgccagcg
     3001 ccgtctccag gccggtcgtc ccggggcgag cgtggtcgaa ctcgacctcc ttgtcctcga
     3061 cggcgtgggg ggcgtgatcg gtggccacgg cgtcgatcgt cccgtcggcc agcccctgcc
     3121 ggagaccgtc gcgatcctcc tcggaccgga tcggggggtt caccttgaac cttgtgtcgt
     3181 acgtgaccag ggcctcgtcg gtgagggcga ggtggtgggg ggtggcgtcg gccgtgaccc
     3241 gcaccccctc ggctcgggcc cgcctgatca gctccaccga tcccgccgac gaggtgtggg
     3301 tgacgtggag ccggcccccg gtgagccgag cgaggaggat gtcccgggcc accgtgacct
     3361 cctcgccctc gcgggggaag cctcggaggc cgagcagcgc cgagtagtgg ccctcgtgca
     3421 tctgggcctc ctcggccagc gtgtcgtcct gggcgtgctg gcagatcacc acgtcgaagg
     3481 ccttcgcgta ctcgagcgcc agccgcatcg tgcgggccga ctgcaccgac cggtggtcgt
     3541 cggtgaacag acggacgccg gccgcggcca gctcgcccat ctcgacgagc gactccccct
     3601 ccagcccctt cgtgatcgcc gacgcgggga tcacctcgac catccccgcc ttctcgccgc
     3661 gcgcggccac ctccaggacg acggcgagct ggtcggccac cgggtcggtg ttcggcatcg
     3721 ccaccaccgc cgtgtacccg cccagcgccg ccgcgcggct gcccgtctcg atcgtctcgg
     3781 cgtcctcgcg gccgggctcg cgcaggtgcg tgtggaggtc cacgagcccc ggcgccacga
     3841 cgaggccgtc gcagtccacc acctcggcgc ccgacgggga cagctcagga ccgacctcct
     3901 cgacgagggt gtccgccacg agcacgtcgg ccgggccgtc caggcccgac gccggatcga
     3961 cgaccctccc tccgcggaag aggatcttcg ccatcagctc gcctccttct gctcaccctc
     4021 caccccgatg atcaggtaga ggagcgccat ccgcaccgcg atcccgttcg tgacctgatc
     4081 cacgatcagc gagcgcggga ggtcggccac gtcggaggcg atctcgacgc cgcggttcat
     4141 ggggccgggg tgcatgatca gcgccacgtc gggtagggcg gccacgcgct ccgcggagag
     4201 cccgtaccgc tgcgagtact cgcgcaccgt ggggaagaac tgctggcgct gccgctcccg
     4261 ctggaccctc agcatgtagc agacgtcgag cttggggagg acggggtcga gctcgtagga
     4321 gacctcggcg ccccaggacg gtgcctcggg cgggatcagc gtgggcgggc ccacgagcgt
     4381 cacatcggcc cccatcttcg tcagggccag cacgcccgat cgagccaccc tcgagtgtcg
     4441 gacgtcgccc acgatcgcca ccttcagccc ctcgaacgac gggaagtgct cccgaatcgt
     4501 gtagaggtcg agcagggcct gcgtggggtg ctcgtgggtc ccgtcgcccg cgttgaggac
     4561 tcgcgcgtcg acccaacgac tcagcgtgta cggcgcgccc gaggaggagt gccggatcac
     4621 gatggcgtcg gcccccatcg cctggagcgt aagcgccgtg tccttcaggc tctcgccctt
     4681 cgagaccgac gacccagagg tcgagaagtt gatcacgtcc gcggacagcc gcttcgcggc
     4741 gagctcgaac gagatgcggg tccgggtgga gttctcgtag aagaggttga cgacggtccg
     4801 ccctcgcagg gccggcacct tcttgatcac gcgggtcccc acctcgcgga acgaatcggc
     4861 cgtgtcgagg atccgggtga cgtcctcggc cgaaagctgc gtgatcgaca agaggtccct
     4921 gatcatgtct cctcccccac ctcgacggcg tcggcgccgt cggtctcgga cgtcaggacc
     4981 cggacctgat cctccgtccg ggtcgggatg ttcttgccca cgaagtcggc tcggatcggg
     5041 agctcgcggt ggccccggtc cacgagcacg gcgagctgga tcgcgcgggg ccgtccgaag
     5101 tccacgaggg cgtccatcgc ggcgcggatc gtccggccgg tgaacagcac gtcgtccacg
     5161 agcacgaccg tgcgcccggt gacgtcgaag tcgatgcggg tctcgtgcac ctccggcgcc
     5221 tcggcccgga gcccgatgtc gtcgcggtag aagctgatgt cgaggacgcc cacggggacg
     5281 tccacgtcct cgatccggcg gagctcctcg gcgatgcggc gggccagatc gtcgcccctg
     5341 gcggcgatcc ccacgaggac gaggtcggcc gccccggcgt tccgctccag gatctcatgg
     5401 gcgatgcgcg tcagggcgcg gcgcacgccg tcggcgtcga gcacgacgcc gctagccgtg
     5461 aaggccacga cccgctcccg gacgcgagaa cgccccaccg cgacacggcg gggcgccctt
     5521 cctctccacg ctgcacgctc cttcccggcc tcacgggacc ggacttaaag ggaagcttct
     5581 tgcgggagcc taccacagag atgcccggtc gcatgacggg cgggggggtg tatcctggct
     5641 gcatggaaac cgtcgcggtg tggacgccgg gtctcggcga gctcatcatc atcctcgtcg
     5701 tcgtcctcct gctcttcggc gcgaagcgcc tccccgagat ggctcggtcg ctggggcgct
     5761 ccaccaagga gttcaaaaag ggcctggacg aggaggccga ggaggaagaa ctccgggagg
     5821 ccgacgagag ccggccggcc ggcgacccgc ccacgccgtc caggtagcgc cttccggcgc
     5881 cgaagcgaga tcccaggggg gcacgtgagg aaggtcatcg tcctcgtcgt cgccaccgca
     5941 gc
//
LOCUS       DASUTW010000117         3870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1461435, whole genome shotgun sequence.
ACCESSION   DASUTW010000117 DASUTW010000000
VERSION     DASUTW010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461435"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2688
                     /locus_tag="VF058_03795"
     CDS             <1..2688
                     /locus_tag="VF058_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX6207461.1"
                     /translation="RCQTAISSFPTAPSLAAVQGRTIEQILPPEILSRFGRRCQEATE
                     RKGPLRYQEHLRVGDEDVVLDAAITPILDAEGASTHLFWSARNVTDEWTTRESLRRTE
                     ERLRTLEREHAKALAGRSEAGEAEAANEERERELEERTRKAEELERQLEERAAEVEAR
                     ERAMEERREGLEGQAGLIEQREQTLEERIRTLDAERRSILENEEAHDEQRKALEDLRK
                     ALEEERASIEAHRAELEHDRSELDEAKAELETRRAEMEAERDTLGGRVVELEHSATEQ
                     EAAAADVEAQRELVETERETLEVLKDSLREERASLEAEKEGLEAEKEALEVRLAQVES
                     RRQAAAGHADHLFRVSTTGVAWSDGDGKLHEVNRALAAMVRRSPEDLIGISLQDLLHA
                     DEQDAIERLLAEARESDEGVAQTETRFAHEDGEISHVHVTLTAVRGEDDEEDRFLWQV
                     ADYTERRGLQERLAHQALHDSLTGLPNRALFLDRLSRALQRLDRNPGTVAVMVMDLDH
                     FAHVNEQNGHEVGDRVLAHVAERLGESIRGSDTVGRLESDTYGILCEELSDAEDAQGV
                     ADRLRRAVEGRAMIDELGVDVGVSIGVALTDETTDRPETLLRDATLAVREAKEGGRGR
                     TEIFDRERREETAQRFRTESDLARAIEEDEFRVFYQPQIDLETGTIVGVEVLVRWQQP
                     DSGLVTPSEFIPLAEETGLIVPLGAWVLEQACLQSKRWTSVAGTELATWVNMSPRQLA
                     QPDLPKMTEKVLAQTGADPATLCVEVTEGIFLEETGGAIANLRALKELGLRVGIDDFG
                     KGFSSLAYLKRLPVDMLKIDRTFVHGLGRSKEDTAIAEAVISLARAMDLTSIAEGVET
                     PEQLARLREMGCDLAQGYLFASPQPAEVIEQLLTQDLRW"
     gene            complement(2691..>3870)
                     /gene="clpX"
                     /locus_tag="VF058_03800"
     CDS             complement(2691..>3870)
                     /gene="clpX"
                     /locus_tag="VF058_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX6207462.1"
                     /translation="GVYICDECIDLCNEIIEEELSETSEIKLEELPKPREIYEFLNDY
                     VIGQDQAKKVLSVAVYNHYKRIQVEGGGGADDVELQKSNILLLGPSGCGKTLLAQTLA
                     RMLNVPFAIADATALTEAGYVGEDVENILLKLIQAADYDVKRAETGIIYIDEIDKIAR
                     KAENPSITRDVSGEGVQQALLKILEGTTASVPPQGGRKHPHQEFIQIDTTKILFICGG
                     SFAGLEKIIESRIGRKGIGFGADVRSSEEKDVGEALALSLPEDLLKYGLIPEFVGRVP
                     VITYVHQLDKGALIDILTKPKNALVRQYHKFFEFDGVELDFTPEALEAIAEQSIRRQT
                     GARGLRAIMEEVLLNVMYDLPGRSDVDRCVIDDQVVMEGVNPTLVPMDEEPERKERSA
                     "
BASE COUNT          639 a         1263 c         1427 g          541 t
ORIGIN      
        1 cgctgtcaga cggccatcag ctccttcccg accgccccga gcctcgcggc cgtgcagggg
       61 cgcacgatcg agcagatcct cccccccgag atcctctcgc ggttcgggcg ccggtgccag
      121 gaggcgaccg aacggaaggg accgctccgg taccaggagc atctccgcgt gggggacgag
      181 gacgtggtcc tcgacgcggc gatcacgccg atcctcgacg ccgaaggagc ctccacgcac
      241 ctcttctgga gcgcgcggaa cgtcaccgac gagtggacca cccgcgagtc gctccggagg
      301 acagaagagc ggctccggac gctcgagcgc gagcacgcca aggccctggc cggccgcagc
      361 gaggccggcg aggcggaggc cgcgaacgag gagcgcgaga gggagctcga ggagcgcacg
      421 cggaaggcgg aggagctcga gcggcagctc gaggagcgcg cggcggaggt cgaggcacgc
      481 gagcgggcga tggaggagcg gcgcgagggt ctcgagggcc aggccggcct gatcgagcag
      541 cgcgagcaga ccctcgagga gcggatccgc accctggacg ccgagcgacg gtcgatcctc
      601 gagaacgagg aagcccacga cgagcagcgg aaggcgctgg aggacctgcg gaaggcgctg
      661 gaggaggagc gggcttcgat cgaggcccat cgcgccgagc tcgagcacga ccgatccgag
      721 ctggacgagg cgaaggccga gctcgagacg cggcgagcgg agatggaggc ggagcgggac
      781 acgctggggg ggcgcgtcgt ggagctggag cactccgcca ccgagcagga ggcggcggcc
      841 gcggacgtgg aggcacagcg agagctcgtg gagaccgaac gggagaccct cgaggtcctc
      901 aaggactcgc tgcgcgagga gcgagcctcg ctcgaggcgg agaaggaggg gctcgaggcc
      961 gagaaggaag ccctcgaggt ccgcctagcc caggtcgagt cgcgtcgaca ggccgcggcc
     1021 gggcacgccg accacctctt ccgggtgtcg acgaccgggg tcgcctggag cgacggggac
     1081 gggaagctcc acgaggtcaa ccgggcgctc gctgcgatgg tgaggcgctc gcccgaggac
     1141 ctcatcggca tctccctgca ggacttgctg cacgccgacg agcaggacgc gatcgagcga
     1201 ctgctggcgg aggcccgaga gagcgatgag ggcgtcgcac agaccgagac gcggttcgcc
     1261 cacgaggacg gggagatctc gcacgtgcac gtgacgctga ccgcggtgcg cggggaggat
     1321 gacgaggagg accggttcct ctggcaggtc gcggactaca cggagcgccg cgggctccag
     1381 gagcggctcg cgcaccaggc cctccacgac tccctgacgg ggctccccaa ccgggcgctg
     1441 ttcctcgacc ggctctccag agccctgcag cgcctcgacc ggaaccccgg gacggtggcc
     1501 gtgatggtca tggatctcga tcacttcgct cacgtgaacg agcagaacgg gcacgaggtg
     1561 ggcgaccgcg tgctggccca cgtcgccgag cggctcgggg agtcgatccg cggctccgac
     1621 acggtggggc gactcgagag cgacacctac gggatcctct gcgaggagct cagcgacgcc
     1681 gaggacgcgc agggggtcgc cgaccgcctc cgccgggccg tcgagggcag ggcgatgatc
     1741 gacgagctgg gcgtcgacgt cggggtcagc atcggcgtcg cgctcaccga cgagaccacc
     1801 gaccgtccgg agaccctcct ccgagacgcc accctggccg tccgcgaggc caaggagggg
     1861 ggacgcggcc gcaccgagat cttcgacagg gaacgccggg aggagactgc gcagcgcttc
     1921 cggaccgaga gcgacctggc ccgcgcgatc gaggaagacg agttcagggt cttctatcag
     1981 ccgcagatcg atctcgagac cggcacgatc gtcggggtcg aggtcctggt tcgctggcag
     2041 cagccggaca gcggcctcgt caccccctcg gagttcatcc cgctggccga ggagaccggg
     2101 ctcatcgtgc cgctcggggc ctgggtcctg gagcaggcgt gcctccagtc caagcgctgg
     2161 acctcggtcg ccgggacgga gctcgcgacc tgggtcaaca tgtcgccccg ccagctggcc
     2221 cagcccgacc tgccgaagat gaccgagaag gtgctcgctc agaccggcgc cgacccggcc
     2281 acgctctgcg tcgaggtgac ggaagggatc ttcctggagg agaccggcgg ggcgatcgcc
     2341 aacctccgcg cgctgaagga gctgggcctg cgcgtgggga tcgacgactt cgggaagggg
     2401 ttctcgtcgc tggcctacct caagcggctc ccggtcgaca tgctgaagat cgaccgaacc
     2461 ttcgtccatg gcctggggcg gagcaaggag gacaccgcga tcgcggaggc cgtgatcagc
     2521 ctggcccggg cgatggacct cacctcgatc gccgagggcg tggagacgcc cgagcagcta
     2581 gcccggttgc gggagatggg gtgcgacctt gcccaaggct atctcttcgc gtccccgcag
     2641 cccgccgagg tgatcgagca gctgctgacc caggacctgc gctggtagcg ctaggcggag
     2701 cgctccttgc gctccggctc ctcgtccatg gggacaaggg tggggttgac gccctccatg
     2761 acgacctgat cgtcgatcac gcaccggtcg acgtcgctgc gccccgggag gtcgtacatc
     2821 acgttcagga ggacctcctc catgatggcc cggaggccgc gggcgcccgt ctgccgccgg
     2881 atcgactgct cggcgatggc ctcgagagcc tcgggggtga agtcgagctc gacgccgtcg
     2941 aactcgaaga acttgtggta ctggcggacg agggcgttct tgggcttggt gaggatgtcg
     3001 atcagggcgc ccttgtcgag ctggtgcacg taggtgatga ccgggacccg ccccacgaac
     3061 tcggggatca ggccgtactt caagagatcc tcggggaggc tgagggcgag cgcctcgccc
     3121 acgtccttct cctcgctgga gcggacgtcg gccccgaacc cgatcccctt ccgaccgatc
     3181 ctcgactcga tgatcttctc cagcccggcg aacgagccgc cgcagatgaa gaggatcttg
     3241 gtggtgtcga tctggatgaa ctcctggtgg ggatgcttcc ggccgccctg gggcgggacg
     3301 ctggcggtgg tcccctcgag gatcttcagc agcgcttgct ggacgccctc ccccgagacg
     3361 tcgcgcgtga tcgaggggtt ctcggccttg cgcgcgatct tgtcgatctc gtcgatgtag
     3421 atgatgcccg tctccgcgcg cttcacgtcg tagtcggcgg cctggatcag cttcaggagg
     3481 atgttctcca cgtcctcacc gacgtagccg gcctcggtga gcgccgtggc gtcggcgatg
     3541 gcgaacggga cgttgagcat gcgggccagg gtctgggcca ggagggtctt gccgcacccg
     3601 gaggggccca gcaggaggat gttggacttc tggagctcga cgtcgtccgc gccccctccc
     3661 ccctcgacct ggatccgctt gtagtggttg taaacggcga cggacaggac cttcttggcc
     3721 tggtcctgtc cgatcacgta gtcgttgagg aactcgtaga tctcacgcgg cttggggagc
     3781 tcctcgagct tgatctcgga ggtctccgac agctcctcct cgatgatctc gttgcagaga
     3841 tcgatgcact cgtcgcagat gtagacgccg
//
LOCUS       DASUTW010000118         7632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1851197, whole genome shotgun sequence.
ACCESSION   DASUTW010000118 DASUTW010000000
VERSION     DASUTW010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7632
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1851197"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VF058_03805"
     CDS             <1..490
                     /locus_tag="VF058_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HEX6207463.1"
                     /translation="EADVVVVGDGPGKVGTATRWGATEVSAGLALQAAAILGARAIAA
                     VRINFADPGYRHHGLSPHSLTVLTRVASPGAHVAVPVLEGRRRDVVWEAIREAKLEER
                     HQVVEVTGQPALDLLAEHSVIAETMGRKLEDEPEFFLAAGAAGVLAGRMAARTERWKA
                     RT"
     gene            490..1161
                     /locus_tag="VF058_03810"
     CDS             490..1161
                     /locus_tag="VF058_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207464.1"
                     /translation="MARTLEVTFNASGAAEAGARGRVVCVVDVLDSSTSAEAALSAGA
                     AAGLGAAPADADPPVTVRPDAVGMRAVALAGQHDASVLVVAEPRVGSQEERQQRAAAV
                     LNGIQGAAIDWELVPNQGAELPALASCEGRIVVVVSTTGGTAFDAAIAAGAPAACFAT
                     TARVGGMPGRDVAEAGVSRAVALCEEHAAGLTIVAASANSLDDVLAAQELARRVIERG
                     FLTFR"
     gene            1223..2935
                     /locus_tag="VF058_03815"
     CDS             1223..2935
                     /locus_tag="VF058_03815"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX6207465.1"
                     /translation="MLTRRDFLKLSAMTGGALALPWRLSGTAFAQTTVPPGSLARYVD
                     ALPIPPVWTAGQLAERGLTMASSMHRFHRDLGMTPTFGYGGASYLGPTIEARRGEPVT
                     YVARNRLGPHVLGIDNELHGPDDYLQDHAAPRVSLHLHGGYTEEGSDGYPEDVFVPGE
                     DHVYHYDMDQQAGTTWYHDHALGITRLNVYAGLAGFFPIRDGAAEAGLPPRPFEVPIA
                     LQDKTFLDPGDGTSPMDYPNPWAPEFFGDVPVVNGKVWPNLAVRRGLYRLRLLDGSSS
                     RFYNLRLDPPVPWYLIGTDVGFVDAPVPIMGNVVLAPGERADVLVDFSSLSPDDHVML
                     MNDQPLPEPVVSPVDDEGPFISELMRFTAVRQRGFPARVPSSLASHPGLDAGAVVAER
                     NLLLVEIADPVTGEPVMALLNNRRWDTADVEQPTVHTLEQWNLINLTEDTHPIHLHLV
                     QFRLRNRQPIEAGPYLEHVFGTEELNPAHVGTGVRPFPSVEEVGVTAGPAVGPPLLED
                     GWKDTIQAHPGQVTRILVPFGSGAAASIPFGRSVTHTGEYVWHCHILDHEDHEMMLPY
                     EVVH"
     gene            complement(2958..4316)
                     /gene="pafA"
                     /locus_tag="VF058_03820"
     CDS             complement(2958..4316)
                     /gene="pafA"
                     /locus_tag="VF058_03820"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HEX6207466.1"
                     /translation="MERRIFGLENEYGVTCTFRGQRRLSPDEVARYLFRRVVHWGRSS
                     NVFLENGARLYLDVGSHPEYATPECDSVEDLVAHDKAGERILERLLEAAELRLHEEGI
                     SGEIFLFKNNTDSAGNSYGCHENYLVARQGEFARMAEVLIPFFVTRQVFAGAGKVLHG
                     PRGATYCLSQRAEHIWEGVSSATTRSRPIINTRDEPHADAERYRRLHVIVGDSNMSEF
                     TTHLKIGVTDLVLRMVEDNTVMRDLTMENPIRAIREISHDITCRKRVRLTNGRELSAL
                     EIQAEYLERAMRYADHRGLDARGMAVLKDWEDVVTALQDRDLDRLRDRIDWVAKWHLI
                     EAYREKHDLPLSSPRVALMDLSYHDVNQDRGLYYLLERKGRMRRAVSEKAVQQAMSDP
                     PQTTRARLRGEFIRQAKLRRRDYTVDWVHLKLNDQAQRTVLCKDPFRAHDDRVQQLIA
                     HM"
     gene            complement(4352..4729)
                     /locus_tag="VF058_03825"
     CDS             complement(4352..4729)
                     /locus_tag="VF058_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX6207467.1"
                     /translation="MRTSTRLGALIAASVLLLAACGGDDDPTVEGGDDAGGAGGTQVA
                     MVDNNFAPSSLELTAGEEVTISTPNNGQNPHSFTIDDLGVDTGELAAGESSEVTFTVP
                     EGETQYYCTVHGAEVMSGTITAS"
     gene            complement(4780..5994)
                     /locus_tag="VF058_03830"
     CDS             complement(4780..5994)
                     /locus_tag="VF058_03830"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6207468.1"
                     /translation="MSVEVRGIRPEEFDDLLRAVATGFGGHINDRDVALERRVMELER
                     TVAAAEGEVFVGGATASSMTLSVPGAIVPAAGITGVSVIPTHRRRGILTRMMRRILDD
                     VREREPLSVLWAAEGGIYGRFGYGMATRLMSLKVERHHSAFRSEYTPQGSMRLVSREE
                     ALKLIPPIYDRVADGQPGFIGQSEAWTDHRFTIYDFLDDGFGKEFFFAIHEGSGGADG
                     YVVYRIKADWAGDEARTLKVEELVAAAPGAYADVWRYVLDVDLVETSIASGRPIHEPL
                     LHVFADPNAAKVDLHDGLWVRLVRVGEALAARRYTTEGRLVLEVDDSFCPWNRGRYEL
                     VGGPEGAECRPTDADPELGVSAELLGAAYLGGVSFRGLARAGLVQASPGVLARADGMF
                     GWDPPPWCPIVF"
     gene            complement(5999..6685)
                     /gene="prcA"
                     /locus_tag="VF058_03835"
     CDS             complement(5999..6685)
                     /gene="prcA"
                     /locus_tag="VF058_03835"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HEX6207469.1"
                     /translation="MTLPFYVAPEQLMKDRSDYARKGIARGKPVVSLEYADGILFIAD
                     NPSTHLHKVSEIYDRIAFAGVGKYSEFENLRIAGVRLADIRGYSYGREDVTAKSLANA
                     YSQALSNVFTQEMKPYEVEILVAQLGDTNDENEMFHILYDGSVTDREGFVAMGGEAES
                     LTERLETSYRDGMDLREALRGGVAALSEANEGGLESSRIEAAVLDRTRKQRRKFRRLT
                     GEQVADHLQG"
     gene            complement(6685..7476)
                     /gene="prcB"
                     /locus_tag="VF058_03840"
     CDS             complement(6685..7476)
                     /gene="prcB"
                     /locus_tag="VF058_03840"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HEX6207470.1"
                     /translation="MTEGIPGSFGANPSFFDLVRAQGMGAPELPGPSEGPGLAPPHGT
                     TVLALRFADGIVMAGDRRAVEGTSVADRRMDKVFPADDYSTVAIAGVAAQAIELVRLF
                     QTELEHYEKVEGEGLSLDGKANRLAQMIRSWMPMVMQGLIVVPVFAGYDLVKREGRIY
                     RYDVIGGRYEGIDFHATGSGGRDAKGSLKKRFRDGLDRAEAIRVALEALLDASEEDTA
                     TGGADVRRGIYPTVYVVTAEGADRVPEDEVRAAHEDVLERRTETG"
     gene            complement(7473..>7632)
                     /locus_tag="VF058_03845"
     CDS             complement(7473..>7632)
                     /locus_tag="VF058_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HEX6207471.1"
                     /translation="AEREAQAEGQTETAAKGEELKEEMDEILDEIDEVLEENAEEFIK
                     SYVQKGGE"
BASE COUNT         1088 a         2720 c         2647 g         1177 t
ORIGIN      
        1 ggaggcggac gtcgtcgtcg tgggcgacgg ccccgggaag gtgggcaccg cgacccgctg
       61 gggagccacc gaggtctccg ccggactggc cctccaggcc gcggcgatcc tcggcgcacg
      121 ggccatcgcc gccgtacgga tcaacttcgc ggatcccggc taccgccacc acgggctctc
      181 cccccactcg ctcacggtcc tgacccgcgt ggcgagcccg ggagcgcacg tggccgtccc
      241 cgtcctcgag ggccggcggc gcgacgtggt ctgggaggcg atccgggagg cgaagctcga
      301 ggagcggcac caggtggtcg aggtcaccgg ccagcctgcg ctcgacctcc tggccgagca
      361 cagcgtgatc gcggagacga tgggccggaa gctcgaggac gagccggagt tcttcctggc
      421 cgccggcgcg gccggcgtcc tggcggggcg gatggcggcc cgcacggagc gctggaaggc
      481 gcgcacctga tggccaggac gctcgaggtg accttcaacg ccagcggggc cgccgaggcc
      541 ggggcccgcg gtcgggtcgt gtgcgtggtg gacgtcctgg actcgtcgac cagcgccgag
      601 gcggcgctgt cggccggcgc ggcggccggt ctcggcgccg cgccggccga cgccgacccg
      661 ccggtcaccg tgcggccaga cgccgtgggg atgcgagcgg tggcgctggc cggccagcac
      721 gacgcctcgg tgctggtcgt cgcggagccc cgcgtgggca gccaggagga gcgtcagcag
      781 cgggccgccg ccgtcctgaa cgggatccag ggtgcggcga tcgactggga gctggtcccg
      841 aaccagggtg cggagctgcc ggccctcgcc tcgtgcgagg ggaggatcgt cgtggtcgtg
      901 tccacgaccg gcgggacggc cttcgacgcg gccatcgcgg ccggggctcc ggcggcatgc
      961 ttcgccacga ccgcccgcgt ggggggcatg cccggccggg acgtcgcgga ggcgggcgtg
     1021 tcacgggcgg tggcgctctg cgaggagcac gccgccgggc tcaccatcgt ggcggcgtcg
     1081 gccaactcgc tcgacgacgt cctcgcggcg caggagctgg cccgccgggt gatcgagcgt
     1141 gggttcctca cgttcaggtg acacgacggt atcgcctagt ctgtgatagg agtccgctga
     1201 ggccgacccg aggaggcgat cgatgctcac ccgccgagac ttcctgaaac tgagcgccat
     1261 gacgggaggg gccctcgcgc tcccctggag gctctccggg acggccttcg cgcagacgac
     1321 cgtgcccccg ggttccctcg cgcggtacgt cgacgcgctc cccatcccac ccgtctggac
     1381 cgcgggccag ctcgccgaac ggggactcac gatggcgtcc tcgatgcatc ggttccaccg
     1441 agatctcggg atgaccccga cgttcgggta cggcggggcc agctacctcg gccccacgat
     1501 cgaggcgcgc cggggagagc cggtcacgta cgtcgctcgg aacaggctgg ggccacacgt
     1561 gctcggcatc gacaacgagc tccatgggcc ggacgactac ctccaggatc acgccgcccc
     1621 tcgagtgtct ctgcacctgc acggcggcta cacggaggaa ggctccgacg gctacccgga
     1681 ggacgtcttc gtccccgggg aggaccacgt ctaccactac gacatggacc agcaggcggg
     1741 gaccacctgg taccacgacc acgccctggg catcactcgc ctgaacgtgt acgcgggact
     1801 cgccgggttc ttcccgatcc gcgacggcgc cgctgaggcc gggctgcccc cgcgcccctt
     1861 cgaggtgccg atcgcgctcc aggacaagac cttcctcgac cccggggacg ggacgagccc
     1921 tatggactac ccgaacccgt gggcaccgga gttcttcggc gacgtgccgg tggtcaacgg
     1981 caaggtctgg cccaacctcg cggttcgccg cggcctctat cggctccgac tcctggacgg
     2041 ctccagctct cgcttctaca acctgaggct ggatccgccc gtgccctggt acctgatcgg
     2101 caccgacgtg gggttcgtgg acgcgccggt ccccatcatg gggaacgtgg tgttggcgcc
     2161 gggcgagcga gcggacgtgc tcgtcgactt cagcagcctc tcccccgacg accacgtgat
     2221 gctcatgaac gaccagcccc tgccggaacc cgtcgtcagc ccggtggacg acgaggggcc
     2281 gttcatctcc gagctcatgc ggttcacggc cgtccgtcag cgcgggttcc cggcgagggt
     2341 cccatcctcg ctcgcgtcgc accccgggct ggatgcgggg gccgtcgtgg ccgagcgcaa
     2401 cctcctgctg gtggagatcg ccgacccggt aaccggcgag ccggtgatgg cgctactgaa
     2461 caaccgtcgc tgggacaccg cggacgtgga gcagccaacg gtgcacacgc tggagcagtg
     2521 gaacctcatc aacctgacgg aggacacgca cccgatccac ctgcacctgg ttcagttccg
     2581 gctgcggaac cgccagccga tcgaggcggg gccgtacctg gagcacgtct tcgggacgga
     2641 ggagctgaat ccggcgcatg tggggacggg cgtgaggccc ttcccgagcg tcgaggaggt
     2701 cggcgtgacc gcgggcccgg cggtgggccc gccgctcctg gaggacggat ggaaggatac
     2761 gatccaagct cacccgggcc aggtcacgcg gatcctcgtg cccttcggca gcggggccgc
     2821 tgcgagcatc ccgttcggcc ggagcgtcac ccacaccggg gagtacgtgt ggcactgcca
     2881 catcctcgac cacgaggatc acgagatgat gctgccctac gaggtggtgc attgagacga
     2941 tcccgtcggg accgcgctca catgtgggcg atcagctgct ggacgcgatc gtcgtgcgcg
     3001 cggaacgggt ccttgcagag cacggtgcgc tgagcctgat cgttgagctt caggtggacc
     3061 cagtccaccg tgtagtcgcg tcgccggagc ttcgcctgtc ggatgaactc cccccggagc
     3121 ctggcccgcg tggtctgcgg cgggtcgctc atcgcctgct ggaccgcctt ctcgctcacc
     3181 gcgcggcgca tcctcccctt ccgctcgagg aggtagtaga ggccgcgatc ctggttcacg
     3241 tcgtggtagg agaggtccat cagcgccacc cgggggctgg acagcggcag atcgtgcttc
     3301 tcgcggtagg cctcgatcag gtgccacttc gcgacccaat cgatgcggtc ccgaagccgg
     3361 tcgaggtcgc ggtcctggag cgccgtcacg acgtcctccc agtccttgag cacggccatg
     3421 ccccgcgcat cgagcccgcg gtggtccgcg tagcgcatgg cgcgctccag gtactcggcc
     3481 tggatctcca gcgcggagag ctcgcggccg ttggtgaggc gcacgcgctt ccggcaggtg
     3541 atgtcgtggc tgatctcgcg gatggcccgg atcgggttct ccatcgtgag gtcccgcatc
     3601 acggtgttgt cctcgaccat gcgcaggacg aggtcggtca ccccgatctt gaggtgggtg
     3661 gtgaactcgc tcatgttcga gtcgcccacg atcacgtgga ggcgacggta ccgctcggcg
     3721 tcggcgtggg gctcgtcgcg cgtgttgatg atgggccggg acctcgtcgt cgccgaggag
     3781 accccctccc agatgtgctc ggctcgctgg gagaggcagt aggtggcgcc ccgcggcccg
     3841 tgcagcacct tccccgcccc ggcgaagacc tgccgcgtga cgaagaacgg gatcagcacc
     3901 tcggccatcc gcgcgaactc gccctgcctg gccacgaggt agttctcgtg gcacccgtag
     3961 gagttcccgg ccgaatcggt gttgttcttg aagaggaaga tctcgcccga gatcccctcc
     4021 tcgtggaggc ggagctcggc ggcctcgagg aggcgctcca ggatccgctc tcccgccttg
     4081 tcgtgcgcca cgaggtcttc caccgagtcg cactcgggcg tcgcgtactc gggatgcgag
     4141 cccacgtcga ggtagaggcg ggcaccgttc tcgaggaaca cgttcgagga gcggccccag
     4201 tggaccacgc gacggaagag gtaccgtgcg acctcgtccg ggctcagccg tcgctggccg
     4261 cggaaggtgc aggtgacgcc gtactcgttc tcgagcccga agattcgccg ctccacccgt
     4321 ccagtgtagg cgcaggcctc ccggatgggc gtcagctcgc cgtgatggtc cccgacatca
     4381 cctcggcgcc gtgaaccgtg cagtagtact gcgtctcacc ctcgggcacg gtgaacgtga
     4441 cctcgctgct ctccccggcc gccagttcgc cggtgtccac acccaggtcg tcgatcgtga
     4501 acgagtgcgg gttctgaccg ttgttgggcg tcgagatggt gacctcctcg cccgcggtca
     4561 gctcgaggga gctgggcgcg aagttgttgt cgaccatcgc cacctgggtg ccgcccgcgc
     4621 cgccggcgtc gtctcctccc tcgaccgtcg gatcgtcgtc gccgccgcac gctgccagga
     4681 ggaggaccga ggcggcgatc agggcgccca aacgcgtgct ggttcgcatc gcttgccttt
     4741 ctcctcgggg acgcccgagt ctaacaacga agctgatttt tagaagacga tcgggcacca
     4801 gggaggagga tcccagccga acatcccgtc cgcccgggcc aggacgcccg gggaagcctg
     4861 aacgagcccg gcgcgggcca gcccacggaa cgagaccccg ccgaggtacg ccgcgccgag
     4921 cagctcggcg gagacgccaa gctccgggtc ggcgtcggtg ggccggcact cggcgccctc
     4981 cgggccgccc acgagctcgt agcggccccg gttccacgga cagaacgagt cgtccacctc
     5041 gagcacgagg cggccttccg tcgtgtatcg acgcgccgcc agcgcctcgc ccacacggac
     5101 gagccgcacc cacagcccgt cgtgcagatc gaccttggcc gcgttcggat ccgcgaagac
     5161 gtgcagcagc ggctcgtgga tcggccggcc cgaggcgatc gacgtctcga cgaggtcgac
     5221 gtcgagcacg tagcgccaca cgtcggcgta ggcgccgggc gccgcggcga cgagctcctc
     5281 caccttcagc gtgcgagcct cgtctcccgc ccagtcggcc ttgatgcggt agaccacgta
     5341 tccgtcggca ccacccgacc cctcgtgaat cgcgaagaag aactccttcc cgaagccgtc
     5401 gtcgaggaag tcgtagatcg tgaagcgatg gtcggtccag gcctcgctct gcccgatgaa
     5461 gcccggctgg ccgtcggcga cgcggtcgta gatcgggggg atgagcttca acgcctcctc
     5521 tcgcgacacc aggcgcatcg atccctgggg ggtgtactcg gagcggaagg cgctgtggtg
     5581 tcgctcgacc ttgaggctca tcagcctcgt ggccatcccg tagccgaacc ggccgtagat
     5641 cccgccctcc gcggcccaca ggaccgagag cggctctcgc tcccggacgt cgtccaggat
     5701 gcgccgcatc atccgagtca ggatcccccg gcgccgatgg gtggggatca cggacacgcc
     5761 cgtgatgcct gccgcgggca cgatcgcccc ggggaccgac agggtcatcg aggaggcggt
     5821 ggcgcctccg acgaagactt ccccttcggc ggcggccacc gtccgctcga gctccatcac
     5881 ccggcgctcg agcgccacgt cccggtcgtt gatgtgcccg ccgaaccccg tcgccacagc
     5941 gcggagcagg tcgtcgaact cctcggggcg gatcccccga acctcgacgc tcatcccctc
     6001 agccctggag gtggtcggcc acctgctcgc cggtcaggcg acggaacttc cggcgctgct
     6061 tgcgggtccg gtcgaggacg gcggcctcga tccgcgagga ctcgagtccg ccctcgttcg
     6121 cctccgagag cgccgccacg cccccgcgga gtgcctcacg gaggtccatg ccgtctcggt
     6181 aggacgtctc gagccgctcg gtcagggact cggcctcgcc gcccatcgcc acgaagccct
     6241 cccggtcggt gacgctgccg tcgtagagga tgtggaacat ctcgttctcg tcgttcgtgt
     6301 cgccgagctg ggcaacgagg atctcgacct cgtagggctt catctcctgg gtgaagacgt
     6361 tcgagagcgc ctgggagtag gcattggcca gcgacttcgc ggtgacgtcc tcccggccgt
     6421 aggagtaccc gcggatgtcg gcgaggcgca cgcccgcgat gcggaggttc tcgaactcgg
     6481 agtacttccc cacgccggcg aaggcgatcc ggtcgtagat ctcgctgacc ttgtggagat
     6541 gggtggaggg gttgtcggcg atgaagagga tcccgtcggc gtactccagc gagaccaccg
     6601 gcttgccgcg cgcgatcccc ttgcgcgcgt agtcagagcg gtccttcatc agctgctcgg
     6661 gggcgacgta gaagggcagc gtcatcagcc cgtctccgtc ctgcgctcca ggacgtcctc
     6721 gtgggccgcc cgaacctcgt cctcggggac gcggtcggcc ccctcggcgg tcacgacgta
     6781 cacggtggga tagatcccgc gccgcacgtc ggctccgccg gtggcggtgt cctcctcgga
     6841 ggcgtcgagg agcgcctcga gcgccacccg aatcgcctcg gcccggtcga ggccgtctcg
     6901 gaaccgcttc ttcaggctgc ccttggcgtc gcggccgccc gatcccgtcg cgtggaagtc
     6961 gatcccctcg tagcggccgc cgatcacgtc gtagcggtag atccggccct cccgcttgac
     7021 caggtcgtag ccagcgaaga ccggcaccac gatgaggccc tgcatcacca tcggcatcca
     7081 cgagcggatc atctgcgcca ggcggttggc cttcccgtcc aacgagaggc cctccccctc
     7141 gaccttctcg tagtgctcca gctcggtctg gaagaggcgg accagctcga tcgcctgcgc
     7201 ggcgaccccc gcgatggcga ccgtggagta gtcgtcggcg gggaacacct tgtccatccg
     7261 gcggtcggcc accgacgtcc cctccaccgc gcggcggtcg ccggccatca cgatcccgtc
     7321 cgcgaaccgc agggcgagga cggtcgttcc gtgaggcggc gcgagcccag gcccctcgct
     7381 cgggcctggg agctcgggcg cgcccatccc ctgggcccgg acgagatcga agaacgacgg
     7441 gttggcgccg aacgagccgg gaatcccctc cgtcattcgc cgcccttctg cacgtaggac
     7501 ttgatgaact cctcggcgtt ctcctcgagg acctcgtcga tctcgtcgag gatctcgtcc
     7561 atctcctcct tgagctcctc gcccttcgcc gcggtctcgg tctgcccctc ggcctgggcc
     7621 tcgcgctcgg ct
//
LOCUS       DASUTW010000119         8073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1851733, whole genome shotgun sequence.
ACCESSION   DASUTW010000119 DASUTW010000000
VERSION     DASUTW010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8073
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1851733"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VF058_03850"
     CDS             complement(<1..261)
                     /locus_tag="VF058_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6207472.1"
                     /translation="MVVDELADEVVDELFRALCDTTRRDILRRSAEDAPSVSRLAEAY
                     PMSFAAVQKHVAVLERAGLVTKERRGREHLVHTVPEAVRRARR"
     gene            complement(317..1549)
                     /locus_tag="VF058_03855"
     CDS             complement(317..1549)
                     /locus_tag="VF058_03855"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX6207473.1"
                     /translation="MPTIEGGLTGPVGATSRIAGVDGFRAVAVLAVLVYHNWLYTAPQ
                     PEDLGYLSRFVFPHLLVGVTMFFVLSGFLLYRPFVARAQTGRRPPSLRHYLRNRALRI
                     FPAYWVILLATGVILPASIVRISTTEVELGRLEPTGLLRNAALVQNYFAGSMDTGILP
                     AWSLAVEWIFYLVLPFLGMLAILVAARTVGGGRTMATLVPPLLLFLMGLVTPWIVDRL
                     EPGTMKDALGRSFLTHGDLFAFGMVLAIVMASIEHGGLRMPRWWRVPAFGLIAALALI
                     AMLLADRELIYIYQGAVIYELLTGPAAMLLLALVVVPSADGSLSLLTRILDSRAFVAL
                     GLISYSLFLWHEPLQRLAFKEGLTLPGVSGFWANLVILGVVATALSVLTYRFIERPAL
                     ALKARPGSGEPADRPSSR"
     gene            complement(1655..2806)
                     /locus_tag="VF058_03860"
     CDS             complement(1655..2806)
                     /locus_tag="VF058_03860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6207474.1"
                     /translation="MRILSTLTYYHPHWTGLTVVAQRLAEGLARLGHEVTVLTSQHDP
                     QLPARERLNGVRVVRVPTIGRVSRTAIMPSFPLALVREVRGSDLVHIHTPMPETPLIA
                     AVARVMRKAVLITHHGDVEMPAGLGNALIKRIMDTVIRTGMRLSDRTVVHVADYRDHS
                     VFLAPVARKIDTIYPPIEVPSPQPERVEAWRQELGLDGRPLVGFAGRFVEEKGFDFLL
                     RAMPLVRARLPEVRFVFAGEKDISYERFFERCRPLYESQREAIVELGLLPDHQQMANY
                     YAMCDVFTLPSRTDCFAIVQVEALLSGTPLVTSDIPGAREVVRVTEAGRLVRMGDPSS
                     IAEGIVQVLQDPDRYRPKQEVVRSVFDPERSLREYEALMRELLPVRGGS"
     gene            complement(2819..4072)
                     /locus_tag="VF058_03865"
     CDS             complement(2819..4072)
                     /locus_tag="VF058_03865"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX6207475.1"
                     /translation="MTTSRTVRRGLDGRLLVVGVVALYLVAVAVADAVWGVDLWRVLG
                     VKSGPTLFFDARNVGAAADCWAQGYDPLYENPCDPWERVIVYPRVWVLFHFLGFTQDR
                     TLLFGWIFVALFLLSLLWLVGRLSLGQGAVVAAAVASPAVMLAIERGNVDILLFALFV
                     VAVFAWQARDRVTPFLSPALIVTAAVAKFYALFALPAFLFTGDRRARWAVATGIGVFG
                     VYVLLTLDDVRQTLQSPEGGLLYSYGARILIGDLYHRFVPGEWGFSSLLAQAIAVVPL
                     AALGVVLWVWARRRITPVTALERLSPRLLAFHLGALTYLGTFAARKNGDYRLVFVLLT
                     LPLLLEWAWGDRGSQRALLGRVGLGVILVGLWVGALSPFIGPWDEVGSWAMAGMFVVL
                     LAATVPPFPLPRALRSRASVEARAG"
     gene            4390..5697
                     /locus_tag="VF058_03870"
     CDS             4390..5697
                     /locus_tag="VF058_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207476.1"
                     /translation="MEPQPQQAPGKWTRIVSGVTRGGTQWLLVGSVLGGIGAYLFQVI
                     GTRALGEEAYAPIGTLWTIQYLFWSIFLFAVETYVTRETVVGRVGREFRGAAAARTWA
                     WIGIVAAALTVASWLARDRLFFGADDLALVAGLTVLSFGAFAIVRGRLAGVGRFKAYG
                     LVSASESLIRLAVAAVVVLVAATTRSLAWTLPSGAAAAACWWFLLRRRRPDAFVDLPR
                     TLEPPRTGRFLTLTTLANGAGQVLLAGGPLALVALAAAPAELSVFFVTTTAARVPVVV
                     ALGGVLSRLLPTFTRVLGERGPEALPGLAGRLAAGTAAVAAVGAIGGAAVGSQLIGFF
                     FGAGFAPPWWLAAVAGGGVLLATGGMLLNQLLIAAGLEHRLPVAWLVALAAGVVVVVV
                     ASGSATARVSAGFIAGELVALCGLIVATRVPSRRPRDAAGRNG"
     gene            5790..6758
                     /locus_tag="VF058_03875"
     CDS             5790..6758
                     /locus_tag="VF058_03875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX6207477.1"
                     /translation="MVVPTRNRSRLLAITLQSILWQRNVELEVIVVEDASTDDTAEMV
                     VGMGDPRITLLRRESPQGPSTARNAGASRASGEWLAFCDDDDLWAPDKLARQVDAATS
                     AGRAWAYVGSVNVGSGFEVIAGRPPPAPEEAVEALRGYNAVPGGGSNVIVRSAIFKEI
                     GGFDDRFPPCEDWELWIRLARAGPPASEARPLLAYRIHGGNSSLDTERILRATRLIEQ
                     VHHTHVDRGLLHRWLAESALRVGRREEALRHMATAAARGEVVGVAGDLLTIARRRAAR
                     LIGKQPGRSSRAESAWIAEASGWLQNLQRLVGASGDGGQRDPQSDP"
     gene            6792..7742
                     /locus_tag="VF058_03880"
     CDS             6792..7742
                     /locus_tag="VF058_03880"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX6207478.1"
                     /translation="MVEIERAGPIFIQGILPRSGTNFLWDLLLLHPDCAAAVEPVRED
                     LFLDHSDHLISFIEAIQDAWDPRWGAPGPELTRRFHASIGEGLISFLWSDPDRRLLTK
                     SPSVRHLRRFFAFFPTARLLILVRDGRAVTQSCMDTFGWGFERAARAWAEGADEIERF
                     GRTNAERSDRWRLVRYEDLVDDLETELPGILDFVGLDPGAYDMEAARNLPVRGSSHFF
                     GPGHSSVHWEPVRKEASFAPNERWRSWSPRQLERFEWIAGHGLRALGYGGGPPGRPLS
                     TAPRHVLLDAWWRTKRAAVHAKDGLAAASRPLRRRLGLAR"
     gene            7739..>8073
                     /locus_tag="VF058_03885"
     CDS             7739..>8073
                     /locus_tag="VF058_03885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6207479.1"
                     /translation="MTPEISAIVPTHNRAGLLTTTLRTVLWQRDVSLEVVVVDDGSSD
                     ATADLVEGLGDPRVRLVRHDAPRGVSTARNRGASEARGDWLAFCDDDDLWAPDKLARQ
                     LAAARDGDRP"
BASE COUNT         1260 a         2839 c         2837 g         1137 t
ORIGIN      
        1 gcggcgggct cggcgcaccg cctcggggac cgtgtgcacg aggtgctcgc gcccgcgccg
       61 ctccttcgtg acgaggccgg cccgctccag cacggcgacg tgcttctgca ccgcggcgaa
      121 gctcatgggg taggcctcgg cgagccgaga caccgaggga gcgtcttccg cggagcgacg
      181 gaggatgtcg cgccgcgtgg tgtcgcaaag agcgcggaag agctcgtcca ccacctcgtc
      241 ggccagctca tctacaacca tttggttgta ggttagcaga atctgagtgt ccatgtgggg
      301 tcgagggcgg ggagccctat cgggaggagg gtcgatcggc cggctcgcca cttcccggcc
      361 gtgccttgag ggcaagtgcc ggccgttcga tgaaccgata ggtcagcacg gagagggccg
      421 tggccaccac gccgaggatg acgaggttcg cccagaagcc agagacgccg ggcagggtga
      481 ggccctcctt gaaggccagg cgctgaagcg gctcgtgcca caggaagagg ctgtaggaga
      541 tcaggcctag ggccacgaag gcccgagagt cgaggatccg ggtcaggagc gagagcgagc
      601 catcggccga gggcaccacc accagggcca gcagcaacat cgcggccggg ccggtcaaga
      661 gctcgtagat caccgccccc tggtagatgt agatcagctc gcggtccgcg agcagcatgg
      721 cgatgagggc gagcgccgcg atgagcccga aggcggggac tcgccaccac cttggcatcc
      781 gcagcccgcc gtgctcgatg ctggccatca cgatggcgag gaccatgccg aacgcgaaga
      841 ggtcgccgtg ggtcaggaag ctgcgcccca gggcgtcctt catggttccc ggctcgagcc
      901 tgtccacgat ccagggcgtc accaggccca tcaggaacag caggaggggc ggtaccaacg
      961 tggccatggt cctgccccct ccaaccgttc gagcggcgac gaggatagcg agcatgccca
     1021 ggaatgggag cacgaggtag aagatccatt cgaccgcgag cgaccacgcc ggcaggatgc
     1081 ccgtatccat ggacccagcg aagtagttct gcacaagcgc tgcgttgcga agtagccccg
     1141 taggctccag ccgcccgagc tcgacctccg tggtcgagat gcggacgatg ctggccggga
     1201 ggatgacccc cgtcgctagc aggatcaccc agtacgccgg gaagatgcgc agggccctgt
     1261 tccgcaggta atggcggagg ctcggaggcc ttcgtccggt ctgcgcacga gccacgaacg
     1321 gccggtagag gaggaacccg gacagcacga agaacatcgt gacgcccacc agaagatgcg
     1381 gaaacacgaa acgactcaga tagcccaggt cttcgggttg cggggccgtg tacagccagt
     1441 tgtggtagac gagaaccgcc agcaccgcca ccgcccggaa cccatcgaca ccggcgatcc
     1501 tggatgtcgc ccctaccggc ccggtgaggc cgccttcgat cgtcggcacc tcggtcgacg
     1561 agcgcagacg tcggcgcttc gctcctctgg caggacgtga tcgtttcctc atgccccgag
     1621 actatgccgc gcctccgtaa gctgactcac ggtatcagga cccgccgcga acgggcagca
     1681 gctccctcat cagcgcttcg tattcgcgca gcgatcgttc cgggtcgaag accgagcgaa
     1741 caacctcctg cttcggccgg tagcgatcgg gatcctggag gacctggacg atcccctcag
     1801 cgatcgacga tggatcgccc atacggacga gccgtccggc ctctgtcacc cgtaccacct
     1861 ctcgggctcc cgggatgtcg gaggtgacca gcggcgtgcc gctgaggagc gcctcgacct
     1921 ggacgatggc gaagcagtcc gtccgagagg ggagcgtgaa cacatcgcac atcgcgtagt
     1981 agttcgccat ctgctgatga tcggggagca gcccgagctc gacgattgcc tctcgctggc
     2041 tctcgtagag cggacgacac cgctcgaaga accgctcgta gctgatgtcc ttctccccgg
     2101 cgaagacgaa ccgcacctcg gggagacgag cccgcaccag gggcatcgcc cggaggagga
     2161 aatcgaaccc cttctcctcg acgaagcgcc ccgcgaatcc gacaagcggt cgcccgtcga
     2221 gccccagctc ctggcgccag gcctcgaccc gttcgggctg gggcgagggc acctctatcg
     2281 gcgggtagat cgtgtcgatc ttccgggcga ccggcgccag gaacacggag tggtcccggt
     2341 aatccgcgac atgcaccacg gtccgatcgg agagccgcat ccccgtccgg atgaccgtgt
     2401 ccatgatgcg cttgatcagc gcgttgccaa gcccggccgg catctccacg tcgccgtgat
     2461 gagtgatcag gaccgccttg cgcatcaccc gggccaccgc cgcgatcagg ggagtctccg
     2521 gcatcggggt gtggatgtgg accaggtcgc tcccgcgaac ctcgcggacc agcgccagcg
     2581 ggaaggacgg catgatcgcc gttcggctga cccgcccgat cgtggggacc cggacaaccc
     2641 ggaccccgtt gaggcgctcc cgcgccggga gctggggatc gtgttgagag gtcagcacgg
     2701 tgacctcgtg cccgagccgc gccaaccctt ccgccagccg ctgggccacc accgtcagtc
     2761 cggtccagtg cgggtggtag taggtgagcg tggagaggat gcgcatccgt cggtccggtc
     2821 agccagccct ggcctcgacg gaggctcgcg agcgcagcgc cctgggcagc gggaaggggg
     2881 gcaccgtggc ggcgagcagg accacgaaca tcccggccat cgcccagctc cccacctcgt
     2941 cccaggggcc gatgaacggc gagagcgccc ccacccacag ccccaccagg atgacgccca
     3001 gaccgacccg gccgagcagc gcccgttggc tgccccggtc cccccaggcc cactcgagca
     3061 ggagggggag ggtgaggagc acgaagacca ggcggtagtc tccgttcttg cgagccgcga
     3121 acgtgccgag ataggtcagg gcgccgaggt ggaaggccaa gagccggggg ctcaggcgct
     3181 ccagggcagt cacgggggtg atccgccggc gggcccacac ccagaggacc acgccgagcg
     3241 ccgccagcgg cacgaccgcg atcgcctggg ccagcaggct gctgaacccc cactcccccg
     3301 gcacgaaccg atggtagaga tcgccgatca gaatccgggc gccgtaggag tagaggagcc
     3361 cgccctccgg cgactgcagg gtctgccgga cgtcgtcgag cgtcagcagg acgtagacac
     3421 cgaagacccc gatcccggtc gccacggccc accgggcccg gcggtccccc gtgaacagga
     3481 aggcgggcag ggcgaacagc gcgtagaact tggccacggc ggcggtcacg atcagcgcgg
     3541 ggctcaggaa cggcgtcacg cgatctctgg cctgccaggc gaacaccgcc accacgaaca
     3601 gcgcgaacag gaggatgtcc acgttgcccc gctcgatcgc gagcatcacg gccggggagg
     3661 cgacggcggc ggccaccacg gccccctggc cgagcgacag ccgtcccacc agccagagca
     3721 gcgacaagag gaacagggcc acgaagatcc acccgaacag gagggttcgg tcctgcgtga
     3781 accccaggaa gtggaacagg acccacaccc gcggatagac gatgacccgt tcccacgggt
     3841 cacaggggtt ctcgtacagg gggtcgtagc cctgcgccca gcagtccgcc gcggctccaa
     3901 cgttgcgggc gtcgaagaag agcgtggggc ccgacttcac gccgaggacc cgccacaggt
     3961 ccacgcccca gacggcgtcg gcgacggcca cggcgacgag gtacagggct acgaccccga
     4021 ccacgagcag ccgcccatca aggcctcgcc gaaccgtcct gctcgtggtc atctcgcgac
     4081 cccctgccgc ctgctcgctg tgagccgcga gcgctcgccc gggaaacacg aggatatatc
     4141 gtcatccccg tggagcggcc ggagcgccca tcgacccccg cccctcgggg gacgggccac
     4201 gcgccgccag ctgctcaacg tggacgctgg gcatcgggct gcgctgttcg gggcggcggg
     4261 ccgccccttc cctacgatgc gcctgcggca ccggacagcg gaggccacgg ctgggatgat
     4321 ccggaagtag cctgataacc ctgcgcctcc cgggtggagg ccaagatggg gaaggggagg
     4381 cagcgaacgg tggagccaca accccagcag gcacccggga agtggacccg catcgtgagc
     4441 ggggtcacca ggggcgggac ccagtggctc ctcgtcggat cggtgctcgg cggcatcggc
     4501 gcgtacctct tccaggtcat cggcacccgc gcgctgggcg aggaagccta cgcgccgatc
     4561 gggaccctgt ggacgatcca gtacctgttc tggagcatct tcctcttcgc ggtagagacc
     4621 tacgtgaccc gggagaccgt ggtcgggcgg gtcgggcggg agttccgggg cgccgccgcc
     4681 gcgcggacct gggcctggat cgggatcgtg gccgcggccc tcaccgtggc ctcgtggctc
     4741 gctcgcgatc gcctgttctt cggcgccgac gacctcgccc tcgtggccgg gctcaccgtg
     4801 ctgtccttcg gggccttcgc gatcgtgcgc ggccggctgg cgggtgtcgg ccgcttcaag
     4861 gcgtacggcc tcgtgagcgc ttcggagtcg ctgatccgcc tggcggtcgc cgcggtcgtc
     4921 gtgctggtgg ccgcgaccac tcgctcgctg gcgtggaccc tcccgtcggg ggccgcggcg
     4981 gcggcctgct ggtggttcct cctccgacgc cgccggcccg acgcgttcgt cgacctcccc
     5041 cgaacgctgg agccgccccg gacgggccgg ttcctgacgc tgaccacgct ggccaacggc
     5101 gccggtcagg tcctgctcgc cggcgggccc ctcgccctgg tggctctcgc cgccgccccc
     5161 gcggagctct cggtgttctt cgtcaccacc accgccgccc gcgtccccgt ggtcgtggcc
     5221 ctgggcggcg tgctatcccg gctccttccc acgttcacgc gggttctcgg cgagcgtggc
     5281 ccggaggccc tccccggcct ggcgggccgg ctcgccgcgg ggaccgcggc cgtggccgcc
     5341 gtcggcgcca tcggcggcgc cgcggtcggc agccagctga tcggattctt cttcggcgcc
     5401 gggttcgctc cgccctggtg gctcgcggcc gtcgcgggtg gcggcgtcct cctcgccacg
     5461 ggagggatgc tcctcaacca actgctgatc gctgcggggc tggagcaccg cctcccggtg
     5521 gcctggctcg tggccctggc cgccggggtg gtggtcgtgg tggtggcctc ggggtccgct
     5581 accgcacgtg tctcggctgg gttcatcgct ggcgagctgg tggcgctctg cgggctgatc
     5641 gtggccaccc gcgttccgag ccgtcgcccg cgggacgcgg ccgggcgcaa tggttgaatg
     5701 ggtgacgaga tgaacgctcc actcttcttt cgccgccacg acgccgacac gcgggcgcac
     5761 acgggctgag cgcgatgacg agcgtctcgg tggtcgtccc gacgcgcaac cgaagccggc
     5821 tcctcgccat cacgctccaa agcatcctgt ggcagcgcaa cgtcgagctc gaggtaatcg
     5881 tcgtggagga tgcctccacc gacgacacgg cagagatggt cgtaggcatg ggcgatcccc
     5941 gtatcacact gctccgtcgg gagtccccgc aggggccgag tacggctcgg aacgccgggg
     6001 cctcccgagc gagcggcgaa tggctggcat tctgcgacga cgacgatctg tgggcgccgg
     6061 acaagctggc ccgccaggtc gacgccgcca cgagcgccgg tcgagcgtgg gcgtacgttg
     6121 gtagcgtcaa cgttgggagc ggcttcgagg tcatcgcggg gcgccccccg cccgcgccgg
     6181 aggaggccgt cgaagcgctt cgtggataca acgctgtgcc ggggggcggc tccaacgtga
     6241 tcgtgcggtc agcgatcttc aaagagatcg gcggcttcga tgaccggttc ccgccatgcg
     6301 aggactggga actctggatc cggctggccc gagccggacc ccccgcctcc gaggcccggc
     6361 ccctgctcgc ctacaggatc cacggcggga attcctcact cgacaccgag cggatccttc
     6421 gagcgacgcg tctcatcgag caggtccacc acacccacgt ggaccggggt ctccttcaca
     6481 ggtggctcgc ggagtctgcc ctcagagtgg gccgacggga ggaggcgctg cgacacatgg
     6541 caaccgcggc cgctcggggc gaggtcgtgg gagtggctgg agacctcctg acgatcgctc
     6601 ggcgaagggc ggcgcgcctc atcgggaagc agccgggccg ctcgtcacgg gcagagagtg
     6661 catggatcgc cgaggcctcg ggctggctcc agaaccttca gaggctggtc ggggcctctg
     6721 gagacggggg ccagcgtgac ccccagagtg atccatgagc tagcatgctc gtcggaagcg
     6781 gagggggcga aatggtcgag atcgagcggg cgggcccgat cttcatccag gggatcctgc
     6841 cccgtagcgg gacgaacttt ctctgggacc tcctcctgct ccaccccgac tgcgctgccg
     6901 ccgtcgagcc ggtccgcgag gacctcttcc tggaccactc ggatcacctc atctccttca
     6961 tcgaggcgat ccaggacgcg tgggatccgc gatggggcgc gcccggcccg gagctcaccc
     7021 ggcggttcca cgcctcgatc ggcgagggac tcatctcgtt cctgtggtcc gatcccgacc
     7081 gccggctcct cacgaagtcc cccagcgtcc gccacctccg gcgcttcttc gccttcttcc
     7141 ccaccgctcg cctcctgatc ctggtccggg acgggcgggc cgtcacgcag tcctgcatgg
     7201 acactttcgg atgggggttc gagcgggcgg cgcgggcctg ggcagagggt gccgacgaga
     7261 tcgagcgatt cggtcggacg aacgcggaac ggtccgatcg atggcgcctg gtgcggtacg
     7321 aggacctcgt cgacgacctc gagaccgagc tccccggcat cctcgacttc gtcgggctcg
     7381 accccggcgc gtacgacatg gaggcggcgc ggaacctccc cgtccgcggc tcctcgcact
     7441 tcttcggccc cggccactcc tcggtccatt gggagcccgt tcggaaggag gcgtcgttcg
     7501 ccccgaacga gcggtggcgc tcgtggagcc cccggcaact ggagcggttc gagtggatcg
     7561 ccggccacgg ccttcgggcg ctcgggtacg gtggcggtcc accgggccga cccctcagca
     7621 ccgccccccg gcacgtgctc ctggacgcct ggtggaggac caagcgcgcc gccgtccacg
     7681 cgaaggacgg gctcgccgcg gcgtcgcggc cgctccgtcg ccggctcggg ctggctcgat
     7741 gaccccagag atctccgcga tcgtccccac gcacaaccgt gcaggcctgc tcaccacgac
     7801 cctgcggaca gtcttgtggc agcgggacgt ctcgctcgag gtggtggtgg tggacgacgg
     7861 ctcctcggac gcgaccgccg acctcgtcga ggggctcggc gacccgcgcg tccggctggt
     7921 ccggcacgac gccccccggg gggtgagcac cgctcggaac cggggtgcct cggaagcccg
     7981 cggcgactgg ctcgccttct gcgacgacga cgatctgtgg gcgcccgata agctggcccg
     8041 gcagctcgcg gcggcccggg acggggaccg gcc
//
LOCUS       DASUTW010000120        17761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_944676, whole genome shotgun sequence.
ACCESSION   DASUTW010000120 DASUTW010000000
VERSION     DASUTW010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 17761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17761
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944676"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..413
                     /locus_tag="VF058_03890"
     CDS             168..413
                     /locus_tag="VF058_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017620305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HEX6207480.1"
                     /translation="MEPLDERSRAILDFERSWWQLPGRKEAAIRRRFGFSAPRYYRLL
                     NRLIDTPEALRYDPMLVRRLRRLRAERRRSRFERTMG"
     gene            complement(972..1793)
                     /locus_tag="VF058_03895"
     CDS             complement(972..1793)
                     /locus_tag="VF058_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207481.1"
                     /translation="MRRLASLLLLASILLPACRDPHVSLGYRLEDGAHLRYSLRLLAD
                     VERTLDDETRLERVEAVFRASQEVLETFPDGGGRMGVTLDPVSLTVNGEPQSAGSAQD
                     FVVVVSPGGEIEEIEETVGAGPAPLAPVGVDRILLRLRPVLPEGPVIPGDAWTSGAVL
                     TDEDGSFSLEANSRLAALGQLDGRRAALVRTTYSSPVDRREVFANAIADLRGRDVGTQ
                     EAWFSLEGFLVRARGDSLGRYAVTFRPPGGQDDIEPVQGRLRVRLRTEMRLLPWP"
     gene            complement(1804..3237)
                     /locus_tag="VF058_03900"
     CDS             complement(1804..3237)
                     /locus_tag="VF058_03900"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX6207482.1"
                     /translation="MNPGILVASNRGPVSFVEEDGRLRATRGGGGLVTALTGVVHETE
                     GMWIAAAMSDEDRKRIEGVPQGRIEVVLEDEKYRLRLLSFDPETYERFYNTVSNRLLW
                     FLHHYLWDVPRWPRFGTPSRVSWDAYRTVNLAFAEALAEEAGESGATALIQDYHLSLV
                     PAQLRARAPETKVAYFHHCPFAGPGYMRLVPTWLREEMLDGLLGADLVGFQSARWAED
                     FLFSARMLPDAEIDLERRTVTWRGRETKVGVYPITVDPEPMREHATSPAVEAKRRQLT
                     EWRGDRKLLLRVDRAELSKNILRGFLAFEDLLQRHPEWRERVVFLAHITPSRTALPEY
                     QEYTQQCIDVASRINDEMGTPGWIPIHIDLSDDLDQAYAAYSLYDVLLVNPVFDGMNL
                     VAKEGPLLNEGHGVLVLSRNAGAIAELDRWALPVDPVDIAGTADALHAALGMPDEERR
                     WRARGLRAAAEARTPADWIGEQLRDLG"
     gene            complement(3234..4481)
                     /locus_tag="VF058_03905"
     CDS             complement(3234..4481)
                     /locus_tag="VF058_03905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6207483.1"
                     /translation="MLISVPVQETRSLDDYVPSAGEDAVRRLREAAEPLRGARLVHIS
                     STAFGGGVAELLPTQIAIMRDLGMEVDWQLIEGSDDFFAVTKLVHNALQGADMPWNGT
                     MASTYLERVDANAQRWVDDADFVIVHDPQPLAIASFVEERERKGGRWVWRCHIDLSKP
                     MEEVWAFYSRFLGSYDASIFTLEDYVHEGAYSNAHLIPPSIDPLSPKNHDLEDEVVEE
                     VLTSYEVDPARPIVTQVSRFDPWKDPLGVMDAFRIAREQVPGIQLVMVGSMAHDDPEG
                     WHYLELAEEHRDGDPDIHLLTNLQDVGALAVNAFQRASTVVMQKSIREGFGLTCTEGM
                     WKERAVIAGRVGGLRLQIEDGHSGFLVDTIEEAADRLIELLRDREAREGMGKAARERV
                     RQRFLSTREVYDHIRLLASLGTR"
     gene            complement(4504..4932)
                     /locus_tag="VF058_03910"
     CDS             complement(4504..4932)
                     /locus_tag="VF058_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6207484.1"
                     /translation="MRVLIIDDEPDVILLCRVNLEFEGHEVLEAGSGEEGLEIAEKEL
                     PDLIVLDLMLPRQDGFSVLRRLHSHEPTKHIPVVLLTAKSREDDQMRGWQTGADEYVT
                     KPFSPVALTEAVARVAGMTAEEREARRRERLEQLSLLHRL"
     gene            5040..6353
                     /locus_tag="VF058_03915"
     CDS             5040..6353
                     /locus_tag="VF058_03915"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX6207485.1"
                     /translation="MAEERKGPLWTPGVSRAARWGIIAWSAIGVLLILYWAFRYVIHP
                     VRVIFPPLVLALIVVYLLNPIVSWLEGRGIPRVIGALLTYLVLLGALGVGLRYLIPVL
                     VEQAQDFSRSLPSLLGRAQEAISDVLQRFGAEGDPGGLLDSGQVRAVTERLFNITIGL
                     LNGAIILVIGLILGVYLLIDIPKIQRGIVALVPERRRPEVLTIGQRVGRAIGGFFRGQ
                     LLVALFVGVLSTLALYIVGLPYWALVGMITGLFNIIPLIGPFIGGAIAVFVAFATDAP
                     GEGLLQLEAGWPLAIGSAIALVAVQQLDNHIVSPNVVARTVKMHPVTVMLALLAGGTL
                     LGLWGMLLAVPVFASIKILALHAWDTQMTWPPRPPPTSAEVESSAPPPEPDAPPPSPP
                     PRPTSRLSSWVDRWRSGRRRTAGSGRQEEAPSDEEQASKGAEERL"
     gene            complement(6350..7186)
                     /locus_tag="VF058_03920"
     CDS             complement(6350..7186)
                     /locus_tag="VF058_03920"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX6207486.1"
                     /translation="MRRAGDVLRGGRRPRVIYTDLDGTLLGPGGSLFAGPEGGVSNAA
                     ANALVALYEAGIDVVPVSGRTAEQVWEVARAIGASDFVAEMGGILSLDRDAERIRQDG
                     AYRGAATPHEAMVEAGAPGMLLEAFPRRLEPHAPWAFLPRVTSMLFRGYLETREAEAA
                     LEEAGYGWLTLLDNGVIPRSFPRLDVEEVHAYHLLPRGVTKATGVGAHVDRRGLDPEE
                     CLAVGDSLSDAALTETVGNVVIVANGWEAVEASGRAPGGLLATEASYGEGFAEVVAEV
                     LS"
     gene            complement(7183..8142)
                     /locus_tag="VF058_03925"
     CDS             complement(7183..8142)
                     /locus_tag="VF058_03925"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HEX6207487.1"
                     /translation="MTAERWFARHTFHHSRFDDVEGLVEAKERQEVSISACIPTLNEA
                     ETIGPIVSGIHRELIDRLPLLDELVVMDSASEDGTARVAEGAGAAVVQDAEVLTGLEP
                     ASGKGEALWKSLFVLKGDLIVWLDADIENFHPRFVYGPLGPLLTDPEIAYTKAFYDRP
                     IKVGDQLQWAEGGRVTELAARPLLNMFWPELAAVVQPLSGEYAGRRSILEQLPFFTGY
                     GVEIGLLIDIAARYGVDSIAQVDLDVRVHRNQDIAALSRMAFAVLQAAMRRLEAAGRI
                     TLAEEPGTSYLQPTGTTERKLERRELRLAERPPAVTIPGYASR"
     gene            8216..8689
                     /locus_tag="VF058_03930"
     CDS             8216..8689
                     /locus_tag="VF058_03930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX6207488.1"
                     /translation="MLKISQKTEYAIRAVVELALRAGQGPVPAREIAGAQGIPLRFLE
                     QQLSALAKAGLIESLRGAGGGAQLAKDPSQIRVADIVDAIEGPFFPMYCLDPSDHTCF
                     QDSRCGIQGLWGEVQAAVRVVFERMTVADLALRHRHIAPESAMWSPEDLLKPSRS"
     gene            complement(8702..8854)
                     /locus_tag="VF058_03935"
     CDS             complement(8702..8854)
                     /locus_tag="VF058_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020525294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEX6207489.1"
                     /translation="MATYEGYCVKDKKKVEFEGEVVTLKNGRRAAKGKCPLCGTTVMR
                     ILGKND"
     gene            9045..10667
                     /gene="groL"
                     /locus_tag="VF058_03940"
     CDS             9045..10667
                     /gene="groL"
                     /locus_tag="VF058_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006542645.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX6207490.1"
                     /translation="MAAKIISFDEHARRALERGMDQLANAVRITLGPKGRNVVLEKKW
                     GAPTITNDGVSIAKEIELDDPEESVGAELVKEVAKKTNDVAGDGTTTATVLAHAMVKE
                     GLRNVAAGANPMGLKRGIETAVERAVEAIKNQAREIEAKEEIAHVAAISAGDPEIGEI
                     IAEAMDKVGKDGVVTVEESQTFGMELDLVEGMRFDKGYISAYFVTDPERMEVVLEEPY
                     VLLANQKISAVKDLLPILEKVMQSGKPLVIIAEDVEGEALATLVVNKIRGTFKSAAVK
                     APGFGDRRKAMLQDIAILTGGQVVSEEVGLKLENAQLDILGQARKIVITKDETTIVEG
                     AGSEDEIKGRINQIKNEIDATDSDYDREKLQERLAKLSGGVAVIKVGAATEVELKEKK
                     HRIEDAVSATKAAVEEGVVPGGGVALLAAQDALDKVDLEGDEATGARLVRKALEEPLK
                     QIAENAGLEGGVVVEKVRALDAGEGLNAATGEYEDMMKAGVIDPAKVTRSALQNAASI
                     AALFLTTEALVAEKPEKQPPMPAMPDAGGMDF"
     gene            10766..10996
                     /locus_tag="VF058_03945"
     CDS             10766..10996
                     /locus_tag="VF058_03945"
                     /inference="COORDINATES: protein motif:HMM:NF020940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HEX6207491.1"
                     /translation="MRRRFHLTFPGHLAGEPVLFLVGKEAGVTPNIRRASVEEGGAWM
                     IVELDGDEASISDAVARLTDRGVVVRRVDEDE"
     gene            10996..11364
                     /locus_tag="VF058_03950"
     CDS             10996..11364
                     /locus_tag="VF058_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6207492.1"
                     /translation="MAKKRILLIDHRPEHMRQPVLRLQLEGYEVDEASDGKTGIEKLR
                     ARRFDLLLLDAELPGSDGWETLRAIREDEELADLKVIIFMAGKGETGKLHLLPVDAEL
                     RRPFPIGELLEKVHRVLGDA"
     gene            complement(11369..12367)
                     /gene="galT"
                     /locus_tag="VF058_03955"
     CDS             complement(11369..12367)
                     /gene="galT"
                     /locus_tag="VF058_03955"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEX6207493.1"
                     /translation="MSTLRQDPTTAEWVIIAPHRAARPRGAASVERPAGLLFDADCPF
                     CPGNEERTPPEIARTERDGSWDQRVFANLYPALQGNGSTERRGNALFREMPGVGQHEV
                     IVESPRHDERLDDMETERLARALGVWRDRYTALRERPWVKSVIVFRNFGARAGTSLPH
                     PHSQILGTPVFAPDALRRYSVATRYFDDTGHCVYDDLREAELGARDRVVFENETFVAL
                     APFASRNPYETWILPRRHRPSFGDLPDDELEGLAQALSRVLRALREGAGDPDYNLIVQ
                     SAPAHEEGKPFFLWHLQLFPRTTTPAGFELGSGMSINPMAPEQAAATLREAIAVRP"
     gene            12444..13262
                     /locus_tag="VF058_03960"
     CDS             12444..13262
                     /locus_tag="VF058_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6207494.1"
                     /translation="MVIDVSERDFEERVLKASREVPVIVDFWAGWCRPCLVLGPILEG
                     LAHEHEGRFVLAKLDVDANQELAARYGIQGIPAVKAFRDGEVVSEFVGAQPEDAVRLW
                     LREVLPSEAEPIVAEAAGLEETGDPERAEGAYRRALEIDPDHEEATVGLARVLLARGE
                     AAEAGRVASRLPGSVEARAIAARAALVERIPGDGDLDDLRARAETDPEAALAVAAADA
                     ERGQTSEALDRLLGLVAGEMRDRARDLMVTIFESLGDDHPLVREYRPRLASALY"
     gene            13262..13600
                     /locus_tag="VF058_03965"
     CDS             13262..13600
                     /locus_tag="VF058_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEX6207495.1"
                     /translation="MPEVTVEELQRIDIRAGTVVRCEAFPEARNPAYKLWIDLGPEIG
                     ERRSSARITDHYEPEGLVGRQVAAVVNLPTRRIGPFSSEVLVLGFYDDDGTVVLVRPD
                     RSVPNGARLG"
     gene            13603..14007
                     /locus_tag="VF058_03970"
     CDS             13603..14007
                     /locus_tag="VF058_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HEX6207496.1"
                     /translation="MESSRFSVDTTGRLVTDLTGRVRDFARDRGGDGLLNLFLPHATC
                     GLAMMETGSGSEGDLQDAIDGFLPPEDRYSHRHGSTGHGRDHLLPLFISPSLTLPVEG
                     GEVVLGTWQSIVLVDTNRENNRRDVVLSLLRG"
     gene            14031..15275
                     /locus_tag="VF058_03975"
     CDS             14031..15275
                     /locus_tag="VF058_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207497.1"
                     /translation="MTERDGTQGLLDRGRAFFTWASRPIRRMWSSDDPLEDYALVHMG
                     SVAGDTLLAIALADSIFFALPVGEARTQVALYLGLTMAPLAVAGPLLVPLLDRSRFRR
                     AISFGASVGRAVAVLLAARWLDSLLLFPAVFTVLVLTRIHLIAKNGLTVAYAPTGAAL
                     MRANAFLARVAIAGALLAAVPGVVMLQLGRTRGVLVLAALVYAATALLNLRLAPVERH
                     PPRAGEVPRRGRVPALAEAALGAGGLRGAQGFLMALLAFALRDAGAPTYWFGVLLVTG
                     TTGAAIADFLVPRLPTTVREEVVVVGALLAAGLGAFLASQASGLPSLALFVGVAGMST
                     EAGRLAFQSLMQANAPGGSQGRVFVRYEVAFQLAWVMGAFVPAMLSISFRGGFIALAP
                     FYLALGAVYVVRLVRARRLRAG"
     gene            15337..15771
                     /locus_tag="VF058_03980"
     CDS             15337..15771
                     /locus_tag="VF058_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX6207498.1"
                     /translation="MGRTLICVVLLAAGCGGTEPPEAGPTGAPPTEQIQRESHLVAGH
                     DRPELPCLEAGRDDPAPLLEMGDTFFAPTCVVLRGRHDLRLTNAGALEHSFRVRDAGV
                     SVDLEPGASAEVSVEELRGSGSTEFFCRFHASAGMTGTITVT"
     gene            15817..16476
                     /locus_tag="VF058_03985"
     CDS             15817..16476
                     /locus_tag="VF058_03985"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX6207499.1"
                     /translation="MSEPRIEVTENGNYRVLGRLPIYPGRILRSADGSSIDWELDEPY
                     PIRATATLCRCGRSGTKPFCDDTHETIEWDGEETADRGPTSARRKAVPGEGVTLTDDV
                     SLCTTAAFCHDRVTDVWRLTFHRSGEPEVRRRIEGIVARCPSGRLVLETQDGGEVEPE
                     YEPSIVVQEGGPYWVRGRVPIAAVDGRTWEVRNRVALCRCGASGNKPFCDSTHLQIGF
                     A"
     gene            16558..16836
                     /locus_tag="VF058_03990"
     CDS             16558..16836
                     /locus_tag="VF058_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207500.1"
                     /translation="MEVHTYIGKGDVVIWKNPRPKIVSKRRVHDVTSIQPRRWKKRLG
                     FKLSKVLQPGSKVKRKFRRKGTYRYRCARHSAMVDGKCQGMCGFIHVL"
     gene            17124..17555
                     /locus_tag="VF058_03995"
     CDS             17124..17555
                     /locus_tag="VF058_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010059393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEX6207501.1"
                     /translation="MATEKRADVTWRGDLATGRGTIERVGSGVIEGLGVSWPARTGES
                     DDLTSPEELIAAAHASCFSMALSKILADQGHAPDRLLVTATVSFVPGTGITRSALDVT
                     GVVPGMEEAEFGKAAEAAKENCPVSQALKGNVELTVASRLS"
BASE COUNT         2587 a         6052 c         6454 g         2668 t
ORIGIN      
        1 gaggagaaga aggccacgat gcggcaggcg gcgacgatga tcggcgtgca gagggtggcc
       61 gaggcgatcg agacccgcgg cctcttcccg tgacatgtcc ttgaacggcc tatatctcgt
      121 gcctatgctc ggacgcgaca caagatgtag cgatcgagag gaggggcatg gagccgctgg
      181 acgagcggtc ccgggccatc ctggacttcg agcggtcctg gtggcagctc ccgggccgga
      241 aggaggccgc gatccggcgc cgattcggat tctcggcccc gcggtattat cgtctgctga
      301 atcggctgat cgacacaccg gaggcgcttc ggtacgaccc gatgctggtg aggcgtctgc
      361 ggcggctccg agcggagcgc cgtcgctcac ggttcgagcg caccatgggg tgaggagcgt
      421 gtggccggca agcatgaacc cgacgcgaaa aggagcttct acctctcctt ggcgaccgcc
      481 acgctgagag ccgggctcgt catcgccgcg gtcgtcctcg gcgtcttcgt cctgtccagg
      541 gcgttcccgg gggccggaga cgagtcccag ggaggccccg gcccggtcac caccgacggg
      601 gccgaggaga gccccgcccc cgaggagtcc ccgcaggagg acggcggcgg gggtggcggc
      661 ggcgggcagg cgccccaaga ggtcaatctc cgaggcgtga ccgtcaacgt gcagaacggc
      721 acgggcgtca cggggctcgc cgcggacacc gccgaggagc tgcgaggcct cggcctgcgc
      781 atccgggaga tcggggacgc cgcccgccac ttcgacgtga cgaccctcta cttctcgcag
      841 ggctcgcggc ccgaggccga ggaactgaac gcgacgttct tcaacggcga ggcgaacgtc
      901 cagcgcatgc cggcggagct ggaggacgac gtccagatca gcgttgtgct cggtcaggac
      961 tacgccgacg ctcagggcca gggcaggagg cgcatctccg tccgcaggcg cacacggagc
     1021 cggccctgca ccggttcgat gtcgtcctgc ccccccggcg gccggaacgt caccgcatac
     1081 cgacccagcg agtcgccccg tgctcggacc aagaacccct ccagggagaa ccacgcctcc
     1141 tgggtcccga cgtcgcggcc ccggaggtcc gcgatcgcgt tggcgaagac ctcccgtcgg
     1201 tcgaccggcg acgagtaggt ggtccgcacg agcgcggccc tccgcccatc cagctgtccc
     1261 agagcggcca gcctcgagtt ggcctcgagt gagaagctgc cgtcctcgtc ggtgaggacc
     1321 gcgcccgacg tccaggcgtc acccgggatc accggcccct cggggagcac ggggcgcagg
     1381 cgcagcagga tccgatcgac ccccacgggg gccaggggcg cgggcccggc cccgaccgtc
     1441 tcctcgatct cctcgatctc gccgcccggg ctcaccacga cgacgaagtc ctgtgccgag
     1501 ccggcgctct gaggctcgcc gttcaccgtg agcgagaccg gatcgagcgt cacgcccatc
     1561 cggcccccgc cgtcggggaa ggtctcgagg acctcctggc tggcccggaa caccgcctcc
     1621 acccgctcca gcctcgtctc gtcatcgagc gtccgctcga cgtcggcgag gaggcggagc
     1681 gagtagcgca gatgcgcgcc gtcctcgagt cggtacccga gcgagacgtg ggggtcccgg
     1741 caggcgggga ggaggatcga ggcgaggagg agcagggaag cgaggcgacg cacgaccgcc
     1801 atcctagccg aggtcgcgga gctgctcgcc gatccagtcg gcgggcgtgc gggcctcggc
     1861 cgccgcgcgc aggccccggg cccgccaccg gcgctcctca tccggcatcc cgagcgccgc
     1921 gtgcagggcg tcggccgtgc ccgcgatgtc cacgggatcc accgggaggg cccagcgatc
     1981 cagctccgcg atcgctcccg cattgcgcga gagcacgagc accccgtggc cctcgttcag
     2041 cagcggcccc tccttagcca cgaggttcat gccgtcgaag accgggttca cgaggaggac
     2101 gtcgtagagg ctgtaggcgg cgtaggcctg gtcgaggtcg tcgcttagat cgatgtggat
     2161 ggggatccag ccgggcgtcc ccatctcgtc gttgatgcgg gacgccacgt cgatgcactg
     2221 ctgcgtgtac tcctggtact cggggagggc cgtgcgggac ggcgtgatgt gggccaggaa
     2281 caccacgcgc tcgcgccact cggggtgccg ctggaggagg tcctcgaacg ccaggaaccc
     2341 gcgcaggatg ttcttggaga gctcggcccg gtccacgcga aggaggagct tccggtctcc
     2401 tcgccactcc gtgagctgcc gccgcttcgc ctcgacggcc ggggaggtcg cgtgctcgcg
     2461 catcggctcg gggtccacgg tgatcgggta cacgccgacc ttcgtctccc gccctctcca
     2521 ggtcacggtg cgccgctcga ggtcgatctc ggcatccggc agcatgcggg ccgagaagag
     2581 gaagtcctcg gcccaccgcg cgctctggaa ccccacgagg tccgcgccca ggaggccgtc
     2641 gagcatctcc tcgcggagcc aggtcggcac gaggcgcatg tagccgggcc cagcgaatgg
     2701 gcagtggtgg aagtaggcga ccttcgtctc aggcgccctg gcccggagct gggccgggac
     2761 gagcgacaga tggtagtcct ggatcagcgc ggtggcgccg ctctccccgg cctcctcggc
     2821 cagcgcctcg gcgaaggcga ggttgaccgt ccggtaagcg tcccaggaga cccgagaggg
     2881 agtgccgaac cgaggccacc gtggaacgtc ccacaggtag tggtggagga accacaggag
     2941 ccggttgctg accgtgttgt agaagcgctc gtaggtctcc gggtcgaacg agaggagccg
     3001 aaggcggtac ttctcgtcct ccagcaccac ctcgatccgg ccctggggga cgccctcgat
     3061 cctcttgcgg tcctcgtcgc tcatagccgc ggcgatccac atcccctcgg tctcgtggac
     3121 caccccggtg agcgccgtga cgagcccgcc ccctcctcgc gtggcccgaa gccggccgtc
     3181 ctcctcgacg aagctgaccg gaccgcggtt cgaggcgacg aggatgccgg ggttcaccgg
     3241 gtccccagcg aggcgagcag gcggatgtga tcgtacacct ctcgcgtgga gaggaagcgc
     3301 tgacgcaccc gctcccgcgc cgccttcccc atgccctcgc gggcctcccg gtccctgagc
     3361 agctcgatga gccgatcggc cgcctcctcg atcgtgtcca ccaggaagcc gctgtggccg
     3421 tcctcgatct ggaggcggag gccgcccacc cggccggcga tcaccgcccg ctccttccac
     3481 atgccctcgg tgcaggtgag gccgaagccc tcgcggatcg acttctgcat caccacggtc
     3541 gaggcgcgct ggaaggcgtt gacggccaag gcacccacgt cctgcaggtt cgtgagcagg
     3601 tggatgtcgg ggtccccgtc gcggtgctcc tcggcgagct cgaggtagtg ccagccctcg
     3661 ggatcgtcgt gcgccatcga gcccaccatc accagttgga tgcccgggac ctgctcgcgg
     3721 gcgatccgga acgcgtccat cacccccagg gggtccttcc acggatcgaa gcgggagacc
     3781 tgcgtgacga tgggccgggc ggggtcgacc tcgtagctcg tgaggacctc ctcgaccacc
     3841 tcgtcctcga ggtcgtggtt cttgggagag agggggtcga tcgacggggg gatcaggtgc
     3901 gcgttggagt aggctccttc gtgcacgtag tcctcgagcg tgaagatgct cgcgtcgtag
     3961 ctgcccagga accgggagta gaaggcccac acctcctcca tcggcttgga caggtcgatg
     4021 tgacagcgcc acacccaccg cccgcccttc ctctcccgct cctcgacgaa ggaggcgatc
     4081 gcgagcggct gagggtcgtg gacgatcacg aagtccgcgt cgtcgaccca ccgttgggcg
     4141 ttggcgtcca cgcgctcgag gtacgtcgac gccatggtcc cgttccaggg catgtcggcc
     4201 ccctgcagcg cgttgtggac gagcttggtg accgcgaaga agtcgtcgga gccctcgatg
     4261 agctgccagt ccacctccat gccgaggtcc ctcatgatcg cgatctgggt gggcaggagc
     4321 tcggccaccc caccgccgaa ggcggtgctg ctgatgtgga ccagtcgggc cccgcggagc
     4381 ggctcggcgg cctcccggag gcgccgcacg gcgtcttctc cggccgaggg gacgtagtcg
     4441 tcgagggagc gtgtttcctg aacggggacg gagatcaaca tcgcggccca gtctaacggg
     4501 cggtcagaga cggtggagca ggctcagctg ctcgaggcgc tcgcggcgtc gagcctctcg
     4561 ttcctcggcg gtcatcccgg ccaccctggc cacggcctcg gtgagggcga cggggctgaa
     4621 cggcttcgtc acgtactcgt cggcgcccgt ctgccaccct cgcatctggt cgtcctcgcg
     4681 ggacttggcc gtgaggagga cgacggggat gtgcttcgtc ggctcgtgcg agtgcaggcg
     4741 tcgcagcacg gagaacccgt cctgccgggg cagcatgagg tcgaggacga tgaggtcggg
     4801 gagctccttc tccgcgatct cgaggccctc ctcgccgctc ccggcctcga gcacctcgtg
     4861 gccctcgaac tcgaggttca cgcggcacag gaggataacg tccggctcgt cgtcgatgat
     4921 gagcacgcgc accgcgtcac ctcctttccc atccatcggc gcccgagggg gtgccccttg
     4981 agcgcgcggc gcggcgctcg ggccgttcgg gggggagccg acggtatgat gacgcccgga
     5041 tggccgagga gcggaagggc ccgttgtgga cgcccggagt ctcccgcgca gcacgctggg
     5101 ggatcatcgc ctggtcggcg atcggcgtcc tcctgatcct ctactgggcg ttccgatacg
     5161 tgatccatcc cgtgcgggtg atcttcccgc ctctcgtcct cgccctgatc gtggtctacc
     5221 ttctcaaccc gatcgtctcc tggctggagg ggcgcggcat cccgcgggtg atcggggctc
     5281 tcctcaccta cctcgtgctg ctcggggcgt tgggggtcgg tcttcgatat ctgatcccgg
     5341 tgctcgtgga gcaggctcag gacttctctc gatcgctgcc cagcctgctg ggccgggctc
     5401 aggaggcgat cagcgacgtc ctccagcgct tcggggcgga gggggatccc ggcggcctgc
     5461 tggacagcgg gcaggtccgg gcggtgacgg agcgtctctt caacatcact atcggtctgc
     5521 tcaacggggc gatcatcctc gtgatcgggc tgatcctcgg cgtgtacctc ttgatcgaca
     5581 tccccaagat ccagcgtggg atcgtggcgt tggtgccgga gcgccgccgt ccggaggtgc
     5641 tcacgatcgg ccagagggtg ggtcgcgcga tcggggggtt cttccgaggc cagcttctcg
     5701 tggcgctctt cgtcggcgtg ctctcgacgc tcgccctcta catcgtcgga ctgccgtact
     5761 gggcgctcgt ggggatgatc accggcctct tcaacatcat cccgctgatc gggcccttca
     5821 tcggaggcgc gatcgccgtc ttcgtggcct tcgccaccga cgccccgggt gagggtctcc
     5881 tgcagctcga ggcgggctgg ccgctggcga tcggatcggc gatcgcgctg gtcgccgtcc
     5941 agcagctcga caaccacatc gtcagcccga acgtcgtggc ccgcacggtg aagatgcacc
     6001 cggtgacggt gatgctggcc ctgctcgccg ggggcactct cctggggctc tgggggatgc
     6061 tgctcgcggt gccggtcttc gcgtcgatca agatcctggc cctccacgca tgggacacgc
     6121 agatgacctg gccaccgagg ccccccccga cgagcgccga ggtggagtcg tcggcgccgc
     6181 ccccggagcc ggacgcgccg cccccctcgc cgcctccgcg cccaacctcg cggctctcct
     6241 cgtgggtcga ccgatggcgc tcggggcgcc ggaggaccgc gggctccggg cgacaggagg
     6301 aggccccgag tgacgaggag caggcctcga agggcgcgga ggagcgtctc taggagagga
     6361 cctccgccac gacctcggcg aacccctccc cgtaggaagc ctccgtggcc agcaggccgc
     6421 cgggggcccg gcccgaagcc tcgacggcct cccagccgtt ggccacgatg accacgttcc
     6481 cgaccgtctc cgtgagcgcc gcgtcgctga gggagtcgcc cacggccagg cactcctccg
     6541 ggtcgaggcc tcgccggtcg acgtgggccc ccacgcccgt ggccttcgtc acgccccggg
     6601 gaaggaggtg gtaggcgtgg acctcctcca cgtccagcct ggggaatgac cgggggatga
     6661 ccccgttgtc gaggagggtg agccatccgt acccggcctc ctcgagcgcc gcctccgctt
     6721 cgcgggtttc caggtatccc cggaagagca tggaggtcac gcgcggcagg aaggcccagg
     6781 gcgcgtgggg ctcgagccgc cgggggaagg cctcgaggag catcccgggt gcgcccgcct
     6841 cgaccatcgc ctcatgcggc gtcgcggcgc cacgatacgc gccgtcctgc cggatccgct
     6901 cggcgtcccg atccagcgag aggatccccc ccatctcggc cacgaagtcg ctcgcgccga
     6961 tcgctcgggc gacttcccac acctgctcgg cggtacggcc ggagacgggg acgacgtcga
     7021 tgccggcctc atagagggcg acgagggcgt tcgcggccgc gttcgagacg ccgccctcgg
     7081 ggccggcgaa gagggagcct cccgggccga gcagggtccc gtcgaggtcg gtatagatca
     7141 cccgggggcg gcgccccccc cggaggacgt ctcccgccct cctcatctcg aggcgtagcc
     7201 ggggatcgtg acggccggcg gacgctcggc gagccgcagc tctctccgct ccagctttcg
     7261 ctccgtggtc cccgtcggct ggaggtagga ggtgccgggc tcctcggcca gggtgatcct
     7321 gcccgcggcc tcgagccgcc gcatcgcggc ctggagcacg gcgaaggcca tcctggagag
     7381 cgcggcgatg tcctggttgc gatggacgcg gacgtcgagg tccacctggg cgatcgagtc
     7441 caccccgtag cgagccgcga tgtcgatcag caagccgatc tcgacaccgt agccggtgaa
     7501 gaagggcagc tgctccagga tgctgcgccg gcccgcgtac tcaccggaca ggggctggac
     7561 caccgcggcg agctcgggcc agaacatgtt gaggaggggg cgggctgcga gctcggtgac
     7621 ccgacccccc tcggcccact ggagctggtc gcccaccttg atcggtcggt cgtagaaggc
     7681 cttcgtgtag gcgatctcgg ggtcggtcag gagcgggccc agcggcccgt acacgaagcg
     7741 cggatggaag ttctcgatgt cggcgtccag ccagacgatg aggtctccct tgaggacgaa
     7801 gaggctcttc cagagggcct cgcccttgcc gctcgccggc tcgagcccgg tgaggacctc
     7861 cgcatcctgc accacggcgg cgcctgcccc ttccgcgacc ctggccgttc cgtcctcgga
     7921 tgcggaatcc atcacgacca gctcgtccag gagcgggagg cgatcgatga gctcgcggtg
     7981 gatcccggac acgatcggtc cgatcgtctc ggcctcgttc agggtgggga tgcaggccga
     8041 gatcgagacc tcctgccgct ccttcgcctc caccaggccc tcgacgtcgt cgaaccgcga
     8101 gtggtggaag gtgtggcgag cgaaccatcg ctcagcggtc accgctcgat gatatcgggc
     8161 agcgagggcc cgtcgcctcc atcgaatccg acccctgcga tatgattcat gacggatgct
     8221 caagatctcc cagaagaccg agtacgcgat ccgggccgtg gtggagctcg cgctgcgggc
     8281 cggccagggc cccgtcccgg cccgggagat cgccggcgcg cagggcatcc ccctccgttt
     8341 cctcgagcag cagctctcgg cgctggccaa ggccggcctg atcgagtccc tgagaggcgc
     8401 cgggggcggc gcgcagctgg ccaaggaccc ttcgcagatc cgagtggccg acatcgtgga
     8461 cgcgatcgag gggcccttct tcccgatgta ctgcctcgac ccgtccgatc acacgtgctt
     8521 ccaggactct cgctgcggga tccaggggct gtggggagag gtgcaagcgg cggttcgcgt
     8581 ggtgttcgag cggatgacgg tcgcggacct tgcgctgaga caccggcaca tcgcgcccga
     8641 gagcgcgatg tggtcgccgg aggacctgct caagccgagc aggagctagg gggttcgggc
     8701 gtcagtcgtt cttgcccagg atgcgcatca ccgtcgtgcc gcagaggggg cacttgccct
     8761 tggcggcccg acgtccgttc ttgagggtca cgacctcccc ctcgaactcg accttcttct
     8821 tgtccttgac gcagtacccc tcgtaggtcg ccaccggcgc ctccttccga acagtcttca
     8881 gggccgctcc gtgcggcccc tgcgtggaat cgtggcccct cggggggcgg gttgcaagca
     8941 tctccgcgct tgtgctcccc gggtgcggcg cgtgtagaat acggcttagc actcgccccc
     9001 ggagagtgcc agcgttcgga acggaccgac tcaggaggct acgcatggcc gcgaagatca
     9061 tctcgttcga cgagcacgca cgccgcgccc tggagcgcgg catggaccag ctcgcgaacg
     9121 ccgtacgcat caccctgggg cccaagggcc gcaacgtggt cctggagaag aagtgggggg
     9181 cccccacgat cacgaacgac ggcgtctcca tcgccaagga gatcgagctc gacgaccccg
     9241 aggagagcgt cggcgccgag ctcgtgaagg aggtcgccaa gaagaccaac gacgtggccg
     9301 gggacggcac gacgaccgcc accgtgctcg cccacgccat ggtcaaggag ggcctgcgca
     9361 acgtggccgc cggagccaac ccgatgggcc tcaagcgcgg gatcgagacg gcggtggagc
     9421 gcgccgtcga ggcgatcaag aaccaggcac gcgagatcga ggccaaggag gagatcgcgc
     9481 acgtcgccgc gatctctgcc ggagacccgg agatcggcga gatcatcgcc gaggccatgg
     9541 acaaggtggg gaaggacggc gtggtgacgg tcgaggagtc ccagaccttc gggatggagc
     9601 tcgacctcgt cgaggggatg cggttcgaca agggctacat ctccgcctac ttcgtcaccg
     9661 acccggagcg gatggaggtc gtcctcgagg agccgtacgt cctgctggcg aaccagaaga
     9721 tctccgccgt gaaggacctg ctcccgatcc tggagaaagt catgcagtcc gggaagcccc
     9781 tggtgatcat cgccgaggac gtggagggtg aggccctggc caccctcgtg gtgaacaaga
     9841 tcaggggcac gttcaagtcc gcagcggtga aggcgcccgg gttcggcgac cgccggaagg
     9901 cgatgctgca ggacatcgcg atcctcaccg gcgggcaggt cgtctccgag gaggtcgggc
     9961 tgaagctcga gaacgcccag ctcgacatcc tgggccaggc ccggaagatc gtcatcacga
    10021 aggacgagac gacgatcgtc gagggagccg gctccgagga cgagatcaag ggccggatca
    10081 accagatcaa gaacgagatc gacgcgaccg actccgacta cgaccgcgag aagctccagg
    10141 agcggctggc caagctctcc gggggcgtcg ccgtgatcaa ggtcggggcg gccacggagg
    10201 tcgagctgaa ggagaagaag caccgcatcg aggacgcggt ctcggccacg aaggcggcgg
    10261 tagaggaggg cgtcgtcccc ggcggcgggg tcgccctgct ggctgcgcag gacgccctgg
    10321 acaaggtgga cctcgagggc gatgaggcga cgggcgccag gctcgtcagg aaggcgctcg
    10381 aggagccgct gaagcagatc gcggagaacg ccggcctcga gggcggcgtg gtcgtcgaga
    10441 aggtgcgggc gctcgatgcc ggggagggtc tgaacgcggc gaccggagag tacgaggaca
    10501 tgatgaaggc cggggtcatc gatcccgcca aggtcacgcg ctcggccctc cagaacgcgg
    10561 cgtcgatcgc ggcgctgttc ctcaccaccg aggccctcgt ggcggagaag cccgagaagc
    10621 agcctccgat gccggccatg ccggacgcgg gcggcatgga cttctgaggc gaacgcacgg
    10681 agggaggggc cccgcgcgcg gagcgcgcgg ggcccctccc gctccgggac gtgtgcgctc
    10741 ccccagcatg ggagacaatc cgggagtgag gcggcgcttc cacctgacgt tccctggcca
    10801 cctggccggc gagccggtgc tcttcctggt ggggaaggag gccggggtca cccccaacat
    10861 ccgccgggcc agcgtggagg agggaggcgc ctggatgatc gtcgagctcg acggggacga
    10921 ggcgtcgatc tcggatgccg tggcccgcct gaccgaccgg ggcgtcgtcg tgcgtcgtgt
    10981 ggacgaggac gagtgatggc gaagaagcgc atcctgctga tcgatcatcg gcccgagcac
    11041 atgcgccagc cggtgctgcg cctgcagctc gagggctacg aggtcgacga ggcatccgac
    11101 ggcaagaccg gcatcgagaa gctccgggcc cgccgcttcg acctcctcct cctggacgcc
    11161 gagctcccgg gcagcgacgg ctgggagacg ctgcgcgcga tccgcgagga cgaggagctg
    11221 gccgacctca aggtcatcat cttcatggcg ggcaaggggg agacggggaa gctccacctg
    11281 ctgccggtcg acgccgagct gcgcaggccg ttccccatcg gggagctcct ggagaaggtg
    11341 cacagggtcc tgggggacgc ctgagccgtc aggggcggac ggcgatcgcc tcgcggaggg
    11401 tggccgccgc ctgctccgga gccatcgggt tgatgctcat ccccgatccg agctcgaacc
    11461 ccgctggggt ggtggtgcgg gggaagagct ggaggtgcca gaggaagaac ggcttcccct
    11521 cctcgtgggc gggggccgac tgcacgatga ggttgtagtc ggggtcgccc gcgccctccc
    11581 gcagggcccg cagcaccctg ctgagcgcct gggccaggcc ctcgagctcg tcgtccggca
    11641 ggtccccgaa cgaggggcgg tggcgcctgg ggaggatcca ggtctcgtag gggttccgag
    11701 aggcgaacgg cgccagcgcc acgaaggtct cgttctcgaa cacgacccgg tcgcgggccc
    11761 caagctcagc ctcgcggagg tcgtcgtaca cgcagtgccc cgtgtcgtcg aagtaccggg
    11821 tggccaccga gtagcgccgc agggcatcgg gggcgaacac gggggtcccc aggatctgcg
    11881 agtgggggtg tgggagcgag gtgccggcgc gggccccgaa gttgcggaac acgatcacgg
    11941 acttcaccca gggcctctct cgcagcgcgg tgtagcggtc ccgccagacc cccagcgctc
    12001 gggccaggcg ctcggtctcc atgtcgtcga ggcgctcgtc atgccttggc gactccacga
    12061 tgacctcgtg ctggcccacc cccggcatct ctcggaacag ggcgttcccc cggcgctccg
    12121 tgctcccgtt cccctggagc gccgggtaga ggttggcgaa gacgcgctgg tcccacgatc
    12181 cgtcacgctc ggttcgggcg atctcggggg gcgtgcgctc ctcgttgccc gggcagaacg
    12241 ggcagtccgc gtcgaagagg agccccgccg ggcgctccac gctcgccgcg ccccggggcc
    12301 tcgccgcgcg gtgaggggcg atgatcaccc actccgcggt cgtcggatcc tgtctcagcg
    12361 tcgacacgat cgccttaccg taccagcctc tgtgcaggca tctcgctaga tggtatcggg
    12421 agccagcggc tacgcttgat ctcatggtga tcgacgtctc ggagcgggat ttcgaggagc
    12481 gggtgctgaa ggcctcgcgc gaggtcccgg tcatcgtgga cttctgggcg gggtggtgcc
    12541 gcccgtgcct ggtcctgggc ccgatcctgg aggggctggc tcacgagcac gaagggcgct
    12601 tcgtgctggc caagctcgac gtagacgcca accaggagct ggcggcgcgg tacgggatcc
    12661 aagggatccc cgccgtgaag gccttcaggg acggcgaggt cgtcagcgag ttcgtcgggg
    12721 ctcagcccga ggacgcggtg cgtctctggc tgcgcgaggt gcttccctcc gaggcggagc
    12781 cgatcgtcgc cgaggccgcc gggctcgagg agacggggga tccggagcgg gccgagggcg
    12841 cataccgtcg agccctggag atcgatcccg atcacgagga ggccacggtg ggtctggccc
    12901 gagtgctcct ggctcgtggg gaggccgcgg aggcgggtcg cgtcgcatcg cgtctgcccg
    12961 ggagcgtgga ggcgcgggcc atcgccgctc gagccgccct cgtcgagcgg atccccgggg
    13021 acggtgacct cgacgacctc cgggcccgag ccgagaccga tccggaggcc gcgctcgccg
    13081 tggcggcggc cgacgcggag cggggccaga cctcggaggc cctggatcgc ctgctggggc
    13141 tggtcgcggg cgagatgcgc gaccgggctc gggacctcat ggtcacgatc ttcgaatccc
    13201 tcggcgacga ccacccgctc gtccgcgagt accggcccag gctcgccagc gcgctgtact
    13261 gatgccagag gtcaccgtcg aggagctcca gcggatcgac atccgggcgg ggaccgtcgt
    13321 tcggtgcgag gccttccccg aggcccgcaa ccccgcgtac aagctgtgga tcgacctggg
    13381 ccccgagatc ggggagcgtc gatcgagcgc caggatcacc gaccactacg agcccgaggg
    13441 gctcgtcggc aggcaggtcg cggccgtggt gaacctcccg acgcggcgga tcggcccctt
    13501 cagctcggag gtgctcgtgc tcggcttcta cgacgacgac gggacggtcg tgctcgtgcg
    13561 gcccgaccgc tcggttccga acggggccag gctggggtga gcgtggagtc ctcgcgcttc
    13621 tccgtggaca cgaccgggcg gctcgtcacc gacctcacgg ggcgtgtccg cgacttcgcc
    13681 cgggatcggg gcggagacgg gctgctcaac ctcttcctcc cgcacgccac gtgcggcctg
    13741 gccatgatgg agacgggctc gggctccgag ggcgacctcc aggacgcgat cgacggcttc
    13801 ctccctcccg aggaccggta ctcgcaccgt cacggctcga cggggcacgg ccgcgatcac
    13861 ctcctcccgc tgttcatctc gccgtcgctg accctgcccg tggagggggg cgaggtcgtg
    13921 ctgggcacct ggcagtcgat cgtgctcgtg gacacgaacc gcgagaacaa ccggcgggac
    13981 gtggtgctct cgctcctgcg cgggtgaccg cccggcgcta gcatccgccc gtgacggagc
    14041 gcgacgggac tcagggcctc ctcgatcgcg gccgggcctt cttcacgtgg gccagccgtc
    14101 ccatccggcg gatgtggtcc tcggacgatc cgctcgagga ctacgcgctc gtccatatgg
    14161 ggagcgtggc cggcgacacc ctgctggcga tcgcgctggc ggactcgatc ttcttcgccc
    14221 tgcccgtcgg ggaggcccgc acccaggtcg cgctctacct ggggctgacc atggccccgc
    14281 tggccgtggc gggccccctc ctcgtcccgc tgctggaccg gagccgcttc cggcgggcga
    14341 tctcgttcgg ggccagcgtg gggagggcgg tggccgtcct gctggccgct cggtggctgg
    14401 actcgctcct cctgttcccg gccgtcttca ccgtcctggt gctgacgagg atccatctca
    14461 tcgccaagaa cggcctgacg gtggcatacg cgcctaccgg cgccgccctg atgagggcga
    14521 acgccttcct ggcccgagta gcgatcgccg gcgccctgct ggccgcggtc ccgggcgtcg
    14581 tgatgctgca gctggggagg acccggggtg tgctcgtttt ggccgcgctc gtgtatgcgg
    14641 cgaccgcgct gctcaacctg cgcctggcgc ccgtggagcg gcacccgcct cgggccgggg
    14701 aggtcccgcg ccgcgggagg gtccctgcgc tcgcggaggc cgccctcgga gcgggcggcc
    14761 tccgcggagc gcagggattc ctcatggcgc tcctggcctt cgcgctccgg gacgcggggg
    14821 cgccgaccta ctggttcggc gtcctcctgg tcaccggcac gaccggcgcg gcgatcgccg
    14881 acttcctggt gccgcgtctt ccgacgacgg tccgggagga ggtggtcgtg gtgggggcgc
    14941 tcctggcggc gggcttgggg gcgttcctcg cgtcgcaggc ctccgggctc ccttccctcg
    15001 cgctgttcgt cggggttgcg gggatgtcca ccgaggccgg ccggctggcg ttccagagcc
    15061 tgatgcaggc caacgcgccg ggaggatcgc agggccgggt cttcgtccgg tacgaggtgg
    15121 cgttccagct ggcgtgggtg atgggggcct tcgtcccggc gatgctgtcg atctcgttcc
    15181 gcggggggtt catcgcgctg gcccccttct acctcgcgct gggcgcggtc tacgtggtgc
    15241 gtctcgtgcg ggcccgacgg ctccgggcgg gctgagggac gtcgggggga catccgagac
    15301 atggccccgg tggctcctgg gccgtaccct cgagccgtgg gccgcacgct gatctgcgtc
    15361 gtccttctcg cggccggctg tggggggacc gagcctccgg aggccgggcc gacgggcgcg
    15421 cctcccaccg agcagatcca gcgcgagtcc cacctcgtgg ccgggcacga ccgacccgag
    15481 ctcccgtgcc tggaggccgg ccgggacgac ccggcgcctc tgctcgagat gggcgacacg
    15541 ttcttcgctc ccacgtgcgt cgtcctgcga ggccgccacg accttcggct gaccaacgcc
    15601 ggcgcgctgg agcactcctt ccgggtccgg gacgccggcg tgtcggtgga cctcgagccg
    15661 ggtgcgtccg ccgaggtatc cgtcgaggaa ctgcggggat ccggctcgac ggagttcttc
    15721 tgccgcttcc acgcctcggc ggggatgacc ggcacgatca cggtgacctg actccccgcc
    15781 ttccccctcg ggcgtgtggc ggctacactc cccgccgtga gcgagccgcg catcgaggtc
    15841 accgagaacg ggaactaccg ggtcctcgga cgcctcccca tctacccggg acgcatcctg
    15901 cggtccgctg atggcagctc gatcgactgg gagctggacg agccctaccc gatccgcgcc
    15961 accgccaccc tctgtcgctg cggccgctcc gggacgaagc ccttctgcga cgatacccac
    16021 gagacgatcg agtgggacgg cgaggagact gccgaccggg gcccgacgag cgctcggcgg
    16081 aaggccgtgc ccggggaggg ggtgacgctc accgacgacg tctcgttgtg caccaccgcg
    16141 gcgttctgcc acgaccgcgt gaccgacgtg tggcggctca ccttccatcg ctccggcgaa
    16201 ccggaggtcc gccggcggat cgaggggatc gtggcgaggt gtccctcggg tcggctcgtc
    16261 ctcgagaccc aggacggggg cgaggtggag cctgagtacg agccgtcgat cgtcgtgcag
    16321 gagggcgggc cctactgggt gcggggccgg gttccgatcg cggccgtgga cggccgcacg
    16381 tgggaggtgc gcaaccgtgt cgccctctgc cggtgcgggg cctcgggcaa taagcccttc
    16441 tgcgacagca cccatctcca gatcgggttc gcgtgacccc ccttgtcgta gccgggggcg
    16501 accaagactg aaggcactcg atcgaggtca aggccaaggt cggcgcgaac aagtggggtg
    16561 gaggtccaca cctacatcgg caagggcgac gtggtcatct ggaagaaccc ccgaccgaag
    16621 atagtgagca agcggagggt ccacgacgtc acctcgatcc agcccaggcg ctggaagaag
    16681 cggctggggt tcaagctcag caaggtgctc cagccgggga gcaaggtgaa gaggaagttc
    16741 cgcaggaagg gcacgtaccg gtaccgctgc gctcgacact cggcgatggt cgacgggaag
    16801 tgtcagggca tgtgcggctt catccacgtg ctctgagcgg gctccggccg cgcgcgtgac
    16861 ggggagggac ggtctgaccg ttcctcccca tcctgatgtg gaacccgcgg cgggtgctct
    16921 ccaaggggag gatcctggag cacgtccggc ggcccgaatt cgacggcccg agggatcgtg
    16981 gagacctaaa tgggggcgcg tcgccgcaag gtcgacatcg cgggacctcg tctgatccat
    17041 accatccgcg gcgttgggta tagtctgcga ctatccagga tgcagcccga agcggatcag
    17101 ctcgtctcga gcgaggaggc atgatggcga cggagaagcg cgcggacgtg acgtggcggg
    17161 gggatctggc cacgggacgg ggaacgatcg agcgggtcgg cagcggggtc atcgaggggc
    17221 tgggtgtctc gtggcctgct cgcaccggcg agagcgacga cctcacgagc cccgaggagc
    17281 tgatcgccgc ggcgcacgcc tcgtgcttct cgatggcgct ctcgaagatc ctggccgatc
    17341 agggtcacgc ccccgaccga ctgctcgtta cggccacggt gagcttcgtg ccggggaccg
    17401 gcatcacgag gtcggcgctc gacgtcaccg gtgtggtgcc cgggatggag gaagcggagt
    17461 tcgggaaggc ggcggaggcg gcgaaggaga actgccccgt ctcgcaggcg ctcaagggga
    17521 acgtcgagct gacggtggca tcccgcctct cctgaggttc ccgggcgacg atccgtcttc
    17581 gggatgggtc tcttgggtag ttcgagcgca accagaagag gaggtaagcc tcatgcgcac
    17641 gcgacttatc gcgttcatga ccgtggcggt cgtggccctg ggtgttccga gccttgcgtc
    17701 gggcgctccg ggcggggaca tccgcgaggc gtgcggcggt tcgttcggac agctcgtgtc
    17761 g
//
LOCUS       DASUTW010000121         2675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_815000, whole genome shotgun sequence.
ACCESSION   DASUTW010000121 DASUTW010000000
VERSION     DASUTW010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815000"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..468
                     /locus_tag="VF058_04000"
     CDS             136..468
                     /locus_tag="VF058_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010080477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6207502.1"
                     /translation="MGRKPYKSAEEKLQVVLSVLRGEMTQVEAARRLEMSQTTIAKWQ
                     KQFLEGARESLARGDNAAAPASRREQDLQGQVEELTQALGEAYVALRVWRKGGALYPL
                     SRTSKRSG"
     gene            477..1382
                     /locus_tag="VF058_04005"
     CDS             477..1382
                     /locus_tag="VF058_04005"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEX6207503.1"
                     /translation="MPVRRFCRRLGIPHSTWYYWRQATRGGRGVRRWPSPVVDAIEEP
                     AAERAHRWSAWGHRKVHAMLTADGYHVSPSSVERALRRRGLLLPARYLAQRRENARRR
                     RACFLEPPTRRNRVWQMDFTQFETTSAGTWYICGVVDYATKLCLGAPVSGTQTARDAT
                     EALRAAVEAVEARLGRTLLKDCVDRETGELRPVVVVTDNGPAYKSSTFLRFIMSRPEL
                     EHVRTRHHAPETNGVVERFFRTLKYDHLYRLEIPNAAELAEEVDRYLAVYNEIRPHET
                     LGQVPPTSVYLTDPNLFQAGSVQET"
     gene            complement(1355..>2675)
                     /locus_tag="VF058_04010"
     CDS             complement(1355..>2675)
                     /locus_tag="VF058_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6207504.1"
                     /translation="DHLADKELLLLLDNFEQVAQAGPVLEDLLGSASGLTALATSRRP
                     LSVRGEQEYLVPPLDPPDTERLPDLPMLREIDGVRLFLERAASVAPRFEMTEENAPAI
                     AAIVARLDGLPLAVELAATQTKLLSPEEIAARLDERLTTVGTGARAAPQRQRTLRDAI
                     AWSYQLLGEPERRLFARLSVFSGGWTVEAAEAVCEPDSLGPDLLELLGSLVDHSLVRR
                     LETPEAESRYSMLETIREFGREQLRAGGEEEAIARRHGEHFLGLAVEAEPHFESDPAW
                     LARCEAEHDNIRAALRWAIAAGEPDLSQVAAGALWRFWRTRGYLMEARLWFDEVLGLP
                     QGDRPTAARAKALTGAGGIAWWRGEVPESRAAYEEALAIERELGDRRRIAEALYDLAH
                     PLWIQEGLEAAVAVAEESLELFREVGDESGVARALGVPRQVSWTLPA"
BASE COUNT          415 a          947 c          936 g          377 t
ORIGIN      
        1 ccacagaagg ctgcccgtgg tccgggtctc cgggcgccct gcatcgtcgt aggtgtaggt
       61 gctgtgtctt gcgtcaagat ttcttggaca ccttagagtg tgatcgtcat cgagccttcc
      121 ccagggggtc tgaggatggg aaggaagccg tacaagagcg ccgaggagaa gctccaggtc
      181 gtgctctcgg tgctgcgcgg tgagatgacc caggtggagg cggctcggcg cctggagatg
      241 agccagacca cgatcgccaa gtggcagaag cagttcctgg agggcgcgcg ggagtcgctg
      301 gctcgcggcg acaacgcggc ggcgccggcg tcgcggcggg agcaggacct ccagggccag
      361 gtcgaggagc tgacccaggc cctcggcgag gcctacgtgg cgctgcgggt gtggcggaaa
      421 gggggggctc tttaccccct ttcgaggacc tcgaagagat ccggttagag gcctcgatgc
      481 ccgtccggcg gttctgccgc cggctgggga tcccccactc cacgtggtac tactggcgtc
      541 aggccacccg cggcggtcgg ggcgtgcgca ggtggccctc tccggtggtc gacgccatcg
      601 aggagcccgc ggccgagcgg gcgcaccgct ggtcagcatg gggtcatcgc aaggtccacg
      661 ccatgctcac cgccgatgga taccacgtct ccccgagctc ggtcgagcgc gccctcaggc
      721 gccgcgggtt gctgctcccg gctcgctacc tggcccagcg ccgggagaac gcccgccgaa
      781 ggcgtgcctg cttcctggag ccgcccacca ggcgcaaccg ggtgtggcag atggacttca
      841 cccagttcga gacgacgtcg gcggggacct ggtacatctg cggggtcgtc gactacgcca
      901 ccaagctgtg cctgggggcg ccggtctcgg ggacccagac cgcccgggac gccaccgagg
      961 ccctccgggc cgccgtggag gccgtggagg ctcgtctggg ccgaacgctg ctcaaggact
     1021 gcgtggaccg agagaccggc gagctccgcc cggtcgtggt ggtgaccgac aacgggccag
     1081 cctacaagtc ctccacgttc ctgcggttca tcatgagccg ccccgagctc gagcacgtgc
     1141 ggacccgtca ccacgccccc gagaccaacg gggtggtcga gcgattcttc cggaccctca
     1201 agtacgacca cctgtaccgc ctggagatcc ccaacgccgc cgagttggcc gaggaggtcg
     1261 accgctacct cgccgtctac aacgagatcc ggcctcacga gaccctgggc caggttccgc
     1321 cgacgtccgt ctacctcacc gaccccaacc tatttcaggc aggaagtgtc caggaaactt
     1381 gacgcggaac accaagggcg cgggcgaccc ccgactcgtc cccgacctcc cggaagagct
     1441 cgaggctctc ctccgccaca gccacggcgg cctccagacc ctcctggatc cacagggggt
     1501 gcgcgaggtc gtagagggcc tccgcgatgc gtcgccgatc gccgagctcg cgctcgatgg
     1561 ccagggcctc ctcgtaggcg gcacggctct ccggcacctc tccacgccac caagcgatgc
     1621 cccccgcccc ggtgagcgcc ttcgcccggg ccgcggtcgg acggtcgccc tgagggagac
     1681 cgagaacctc gtcgaaccag aggcgggcct ccatgaggta tccgcgggtc ctccagaagc
     1741 gccacagcgc gcccgccgcg acctgggaga ggtccggctc gcccgcagcg atcgcccacc
     1801 gaagggccgc tcggatgttg tcgtgctccg cctcgcaccg ggcgagccag gcgggatcgc
     1861 tctcgaagtg gggctcggcc tcgacggcca gccccaggaa gtgctctccg tgacggcgag
     1921 cgatcgcctc ctcctcgccc cctgcgcgga gctgctcccg gccgaactcg cggatcgtct
     1981 ccagcatcga gtagcgcgat tcagcctcgg gcgtctcgag gcggcggacc agtgaatgat
     2041 cgacgaggga gcccaagagc tcgagcagat ccggcccgag ggagtcgggc tcgcacaccg
     2101 cctcggccgc ctccaccgtc cacccgccgg agaagaccga tagacgagcg aagagtcgac
     2161 gctccggctc cccgaggagc tgatagctcc acgcgatcgc atcccggagg gtccgctgcc
     2221 gctgaggggc ggcccgggcg ccggtcccca ccgtggtgag gcgctcgtcc agccgggcag
     2281 cgatctcctc aggcgagaga agcttggtct gggtggccgc cagctcgacg gccaggggaa
     2341 gcccgtccag ccgcgccacg atcgcggcga tcgcgggggc gttctcctcg gtcatctcga
     2401 accgaggcgc cacggaggcc gcccgctcca ggaacaggcg gacaccgtcg atctcgcgga
     2461 gcatcggcag gtcggggagg cgctcggtgt cgggcgggtc gaggggcggc acgaggtact
     2521 cctgttctcc tctgaccgag aggggcctcc gggacgtcgc cagcgcggtg agccccgagg
     2581 ccgatcccag gagatcttcg agcacgggac ccgcctgggc cacctgctcg aagttgtcga
     2641 gcaggaggag cagctccttg tcggccagat ggtcc
//
LOCUS       DASUTW010000122         5596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_945246, whole genome shotgun sequence.
ACCESSION   DASUTW010000122 DASUTW010000000
VERSION     DASUTW010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945246"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VF058_04015"
     CDS             complement(<1..795)
                     /locus_tag="VF058_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX6207505.1"
                     /translation="MGAIDRLRDSGAWHDVIEGEAGRRVRALTRRTPLVRAPGMLVKA
                     EGLQRTGSYKARGAVAKLATIPAGGEVVAASAGNHGLGVAYAATALGHRATVVVSEAA
                     PEVKRRGIEALGARLLVHGSGYDEAEAHARSLAGSEGALFVGYADDHVIVGNGGTIAQ
                     ELAEQVEGLGEGDAVVIPVGGGGLASGIGAMLRGSGVRLVGVEPETNRAMTISFHEGA
                     ARTSYPEGAPTLAEGLEGAVAENTYELCRESLDEIVLVSEEEIRRAI"
     gene            complement(800..1195)
                     /locus_tag="VF058_04020"
     CDS             complement(800..1195)
                     /locus_tag="VF058_04020"
                     /inference="COORDINATES: protein motif:HMM:NF015917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207506.1"
                     /translation="MGWEDLGGQLTSVAAVAAWGDGEVQVFAVHDDGEVWNRYWDGRR
                     WHDWESLGGSFTGHPAAAARDASQIDVFAVGADGVLRHRWWDGVRWVDWEEVEGAPHD
                     GSAVSCAWVGHRLYLLVTGPDGRLWQTTL"
     gene            complement(1242..2039)
                     /locus_tag="VF058_04025"
     CDS             complement(1242..2039)
                     /locus_tag="VF058_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX6207507.1"
                     /translation="MDRLRFGTYLAPALLPVYEAITEHVGERLGIDTELVVETSYDSC
                     ENDENEVCFVCSLPYVMFERRGVDVALPVAAPVVTDPRYEGRPIYYSDVIVSADAPFR
                     SFDDLRGRSWAYNEPLSHSGYGITRFHLLTMGETNGFFGEVVEAGFHEEAIRMVARGE
                     VDGSAIDSHLLAVELRNDPALAEGLRIVASLGPSTIQPVAVSRRVPPDLRDAIRETLI
                     TMHWDLGMRRRLGKSLIERLVPVDAASYDDIRMMVDVCERAGFLQLR"
     gene            complement(2070..3395)
                     /locus_tag="VF058_04030"
     CDS             complement(2070..3395)
                     /locus_tag="VF058_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207508.1"
                     /translation="MAPAQLSGSFTLIGHEPLFDRGMNAGLAVWGDHAYVGNRTDGSS
                     GHPHPGILVVSVADPAAPEVVGEIGGPHAGGPGETSRELRIWPGGELLIALTFGCDPF
                     LHGCSPDPVRPSFSFYDIGADRAARPRFVSEYQPQGQPHEFYLWQDPRDRGRALLFVS
                     TPTPVGENLLVLDLSEARSGRVRELATWSAEFPDRGPDDALHSLSVSVDGRRAYLAHL
                     TAGFLVLDTSELARGAEEPEIRAITRLEQRARWPGVGPHSAVKIPGRDLILTTDEVYG
                     RSTGGGCPWGWGRLISIAEETAPTVASEFGVPPYNDPSYCEEVPSERDADASFSSHNP
                     TVTEHLALITWHGAGLQAFSTEDPAAPERVAEFLPEPLPTVATEDPALSSGPDKVVMW
                     SYPIVVNGLIHVVDVRNGLYVLEYRGPFEEEVAALSFLEGNSNLGDASP"
     gene            complement(3502..3702)
                     /locus_tag="VF058_04035"
     CDS             complement(3502..3702)
                     /locus_tag="VF058_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207509.1"
                     /translation="MPETYGKRQRKAVKARKASAREERRLARARRREERAAQGESAGD
                     EAWLGEPNPSGLDEPAGDDTEE"
     gene            4061..4636
                     /locus_tag="VF058_04040"
     CDS             4061..4636
                     /locus_tag="VF058_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207510.1"
                     /translation="MQGRAHTSPGGRQRPPRALALVAAAALGAALFGGVAAATHDPDT
                     IHACVHKQTGQVRVVDDASDCKPQETPLEWNVEGPAGPQGPEGPPGPQGEPGGLGGHE
                     VVQSDLILIGPGEKLGEIALCPRGKVVTGGGHTVPGQFDPNGIPPQGVPAVVVESYPV
                     TVPSHGWVVVMRSDGPTASFTMRVRAVCVDG"
     gene            complement(4982..5356)
                     /locus_tag="VF058_04045"
     CDS             complement(4982..5356)
                     /locus_tag="VF058_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207511.1"
                     /translation="MRRKEVRMRRLAIVLALSALFLGALAGTSGARGVTTTQLQHAGW
                     ICFPTGTGPHCFTPGALASSASIQVLVFDDWGSEGILRGSELLIRADLYAGQPCPQSE
                     VEFLPAAETGLSSDYFACHHYG"
     gene            complement(5438..>5596)
                     /locus_tag="VF058_04050"
     CDS             complement(5438..>5596)
                     /locus_tag="VF058_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX6207512.1"
                     /translation="EALSNRQIAERLYLSERTVESHVQSILAKLGMTSRTQVAVWALQ
                     EGLNGAGA"
BASE COUNT          835 a         2061 c         1909 g          791 t
ORIGIN      
        1 gatcgcccgg cggatctcct cctcgctcac cagcacgatc tcgtccagcg actcgcggca
       61 cagctcgtag gtgttctcgg ccacggctcc ctccagcccc tcggccagcg tcggagcccc
      121 ctccgggtag gaggtcctgg cagccccctc gtggaacgag atggtcatgg cgcggttcgt
      181 ctcgggctcg acgccgacga gcctgacccc gctaccgcgc agcatcgccc cgatccccga
      241 ggccagccct cccccgccca cggggatcac gaccgcgtcg ccctcgccca gcccctcgac
      301 ctgctccgcg agctcctgcg cgatcgtccc cccgttgccg acgatcacgt ggtcgtccgc
      361 gtacccgacg aagagtgcgc cctcgcttcc ggccagcgac cgcgcgtgcg cctccgcctc
      421 gtcgtagccg gagccgtgga ccaggagccg cgctccgagc gcctcgatcc cacgtcgctt
      481 cacctcggga gcggcctccg agaccacgac cgtggcccgg tggcccagcg ccgtcgccgc
      541 ataggcgacg cccaggccgt ggttgccggc cgaggcagcc acgacctccc cgcccgcggg
      601 gatcgtggcg agcttggcga cggctccccg ggccttgtag gaccctgtgc gctgcaggcc
      661 ctccgccttc acgagcatcc cgggggcccg aaccaggggg gtgcggcgcg tgagcgcccg
      721 gacgcggcgc ccggcctccc cctcgatcac gtcgtgccag gcgccgctgt cgcggagccg
      781 gtcgatcgct cccatgcggt cagagggtcg tctgccagag acgcccgtcc ggaccggtca
      841 ccaggaggta gaggcgatgg ccgacccacg cgcacgagac ggccgagccg tcgtgaggcg
      901 ccccctcgac ctcctcccag tcgacccagc gcacgccgtc ccaccagcga tgacggagca
      961 cgccgtcggc ccccacagcg aagacgtcga tctgcgacgc atcgcgtgcc gcggcggccg
     1021 ggtgacctgt gaagctccct cccagcgact cccagtcgtg ccagcggcgg ccgtcccagt
     1081 accggttcca gacctcgccg tcgtcgtgga cggcgaagac ctgcacctcg ccgtcgcccc
     1141 acgcggccac cgcggcgacc gaggtgagct gccctcccag atcctcccaa cccatcgcgc
     1201 ctcccgaagg aaccctaccg cccccccgaa ccagacgcag gtcagcgaag ctggaggaac
     1261 ccggcccgct cgcagacgtc caccatcatg cggatgtcgt cgtaggaggc ggcgtccacc
     1321 ggcacgagcc gctcgatcag gctcttcccc agcctgcgcc gcatcccgag atcccagtgc
     1381 atcgtgatca gcgtctcccg gatcgcgtcc cggagatccg gcggcacccg cctcgacacg
     1441 gccacgggct ggatcgtcga gggccccagc gacgccacga tcctcagccc ctccgccagc
     1501 gcgggatcgt tgcgcagctc gaccgcgagc aggtgcgagt cgatcgccga cccgtccacc
     1561 tcgccccggg ccaccatgcg gatcgcctcc tcgtggaacc cggcctccac gacctcaccg
     1621 aagaagccgt tcgtttcgcc catggtcagc aggtggaagc gggtgatgcc gtacccggag
     1681 tgcgagagcg gctcgttgta cgcccacgat cgcccgcgca ggtcgtcgaa cgatcggaag
     1741 ggcgcgtccg cggaaacgat cacgtccgag tagtagatcg gccgcccctc gtagcggggg
     1801 tcggtcacca cgggcgcggc gaccgggagc gcgacgtcga ccccccgccg ctcgaacatc
     1861 acgtagggca ggctgcagac gaagcagacc tcgttctcgt cgttctcgca ggagtcgtag
     1921 gacgtctcca ccacgagctc ggtgtcgatg cccagtcgct cgcccacgtg ctccgtgatc
     1981 gcctcgtaga cggggagcag ggccggcgcc aggtaggtgc cgaaccgcag gcgatccatg
     2041 ccccgaagtc tatgcgcgga tccgggacac tagggcgagg cgtctcccag gttggagttg
     2101 ccctccagga acgagagcgc ggccacctcc tcctcgaacg gtccccggta ctcgagcacg
     2161 tagagcccgt tgcgcacgtc gacgacgtgg atcaggccgt tcacgacgat cgggtagctc
     2221 cacatcacga ccttgtcggg gcccgagctg agggccgggt cctccgtcgc cacggtgggc
     2281 aagggctcgg ggaggaactc cgccaccctc tccggcgcgg ccggatcctc cgttgagaac
     2341 gcctgcagcc ctgccccgtg ccacgtgatc agcgcgaggt gctcggtgac ggtcgggttg
     2401 tgcgacgaga aggacgcatc ggcatcgcgc tccgacggga cctcttcgca gtacgaggga
     2461 tcgttgtacg gcgggacccc gaactccgag gccaccgtgg gggcggtctc ctcggcgatg
     2521 ctgatgaggc gaccccaccc ccacgggcat cctccgccag tcgaccgccc gtagacctcg
     2581 tcggtcgtga ggatcaggtc gcggcccggg atcttcaccg cgctgtgcgg gcccactccg
     2641 ggccatcggg cccgctgctc caggcgtgtg atcgctctga tctccggctc ctcggcgcct
     2701 cgagccagct ccgaggtgtc cagcaccagg aagccggccg tcagatgcgc cagataggcg
     2761 cgccggccgt cgaccgagac cgaaagcgag tgcagggcat cgtcggggcc acgatccggg
     2821 aactcggcgg accacgtggc gagctctcgg acccggccac tcctcgcctc ggagaggtcc
     2881 agcacgagca ggttctcccc gacgggggtg ggggtggaga cgaagagcag cgcgcgtccg
     2941 cggtccctcg ggtcctgcca caggtagaac tcgtggggct gaccctgggg ttgatactcg
     3001 gagacgaagc gaggacgggc cgctcgatcg gctccgatgt cgtagaagct gaaggacggc
     3061 cggaccgggt cgggcgagca tccgtggagg aaggggtcgc atccgaaggt cagcgcgatc
     3121 agcagctcgc cgccgggcca gatccgcagc tcccgcgagg tctccccggg accgcccgcg
     3181 tgcgggcccc cgatctcccc cacgacctcg ggggcggcgg gatccgccac gctcaccacg
     3241 aggatccccg ggtgcggatg cccggaggag ccgtccgtcc ggttgcccac gtaggcgtgg
     3301 tcgccccaca cggcgagccc ggcgttcatc ccgcgatcga agaggggctc gtgcccgatc
     3361 agcgtgaagg agcccgaaag ctgggcgggc gccacccggg tcgcggacgg ggtccggcga
     3421 ggggcgttcg ccgcctcccc gctgcaggcg aaggagagga gggcgaccga gagcgtcagg
     3481 ccgagcgcgc gcccgaggcg ctcactcctc ggtgtcgtcc cccgcaggct cgtccaaccc
     3541 cgaggggttg ggctcgccga gccaggcttc atcccccgcg ctctccccct gggccgctcg
     3601 ctcctcgcgc ctccgggcgc gcgcgagacg gcgttcctct cgcgccgagg ccttccgcgc
     3661 cttcacggcc ttccgctgtc gtttcccgta cgtctccggc atgcccccac cgtatcgcac
     3721 ccgggtcgcc ctggaggcca agggcccctc acgagcgggt ttcgtctgct ctgggccgga
     3781 acgtgcttgg cgccgtcggc ccccttagcc aggcacgcga cgcccgcaag cgcgggaccc
     3841 gactatccga tatgcaatga atcgcgatgg atgcgccttc accactgtcc cggcggacgc
     3901 gctctgctgt gcgctcgcct gcggaccgcg cgtgcctggg tcgggagtcc tcccgtcccc
     3961 cgccgagccc atatggtcgc cctcttctcc cgataccgga gcaacccggc ggcggggttc
     4021 aatcggtgca acccgtcggc gatccgagga gggatggcac atgcaaggac gtgcgcacac
     4081 gagccccgga gggcggcagc ggccacctcg agccctggct cttgtcgccg ccgcggccct
     4141 gggagcggcg ctcttcgggg gcgtggcagc agccacgcac gaccccgaca cgatccatgc
     4201 ctgcgtgcac aagcagaccg gccaggtccg ggtggtggac gatgcgtcgg actgcaagcc
     4261 gcaggagaca cccctcgagt ggaacgtgga gggtccggcg ggaccccagg gacccgaagg
     4321 tccgcccggg ccgcagggcg agcccggcgg gctgggcggc cacgaggtcg tgcagtccga
     4381 tctcatcctg atcgggcccg gcgagaagct cggagagatc gctctctgcc cgcgcggaaa
     4441 ggtggtcacg gggggcgggc acacggtgcc ggggcagttc gacccgaacg ggatcccgcc
     4501 ccagggggtc ccggccgtcg tggtcgagtc ctatccggtc accgttccga gccacggatg
     4561 ggtcgtggtg atgcggagcg acggcccgac cgcgagcttc accatgaggg tccgggccgt
     4621 gtgcgtggac ggctgaggtg cagggggtcg gtggaggggc cagatccgaa caggcgacct
     4681 cgccctcccg acgggatcgt gtcggagggg agagtcgaac tcccacgggg tggttagccc
     4741 cactaggccc tcaacgcagc gtgctcgatt cgccgatgtc tgtcagaggt cgcgctgcag
     4801 gtcagtgagg tcttctcgtg gcagcgaacg ctggcggagg gctctgaatc tgtgtcgatg
     4861 tgcccgagtg tgaccaagac tgtgactacc tcgtcggagc gggcacctgc gccctcagcc
     4921 tcccgggaag ccggaagagc ccaccgccgt ggcgggctct tccggatccg cgcgtggtcg
     4981 gtcagccgta gtggtggcag gcgaagtagt cgctgctcag accggtctcg gctgccggca
     5041 ggaactcgac ctcactctgg gggcagggct gccccgcgta caggtccgcc ctgatcagca
     5101 gctccgaccc ccgcaggatg ccctccgaac cccagtcgtc gaacaccaat acctggatgc
     5161 tcgcgctgga ggcgagcgca ccgggggtga agcagtgcgg cccggtcccc gtcgggaagc
     5221 agatccaccc cgcgtgctgg agctgggtgg tggtcacccc acgagcccca ctcgttccgg
     5281 caagggcccc gaggaacagc gccgacagcg ccagcacgat cgctagtcgc ctcatccgaa
     5341 cctccttccg tcgcatcagg tcggaaggct aggaccaaga gggggcgacg cacatcggtg
     5401 gaagtacggg gatgggtacg gaattccgcg tctcccccta cgcacccgct ccattgagcc
     5461 cttcctggag ggcccagacc gccacctggg tgcgcgaggt catccccagc ttggccagga
     5521 tgctctgaac atggctctcg acggttcgct cggaaaggta gaggcgctca gcgatctgtc
     5581 ggttcgatag ggcctc
//
LOCUS       DASUTW010000123         4050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1721778, whole genome shotgun sequence.
ACCESSION   DASUTW010000123 DASUTW010000000
VERSION     DASUTW010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721778"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VF058_04055"
     CDS             complement(<1..251)
                     /locus_tag="VF058_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HEX6207513.1"
                     /translation="MPNRLQGATSPYLLQHADNPVDWYEWGPEALERARAEDRPILLS
                     VGYAACHWCHVMERESFEDEDTAALMNEHFVNVKVDREER"
     gene            317..565
                     /locus_tag="VF058_04060"
     CDS             317..565
                     /locus_tag="VF058_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207514.1"
                     /translation="MAADEFDERFHEAVTALDEIAGEIKPPEAWDGFDPVSLEEFWQS
                     WPAVRAWGEWLWQLVEAERGEKAAPAEDSPYEEIGGSG"
     gene            complement(538..1614)
                     /locus_tag="VF058_04065"
     CDS             complement(538..1614)
                     /locus_tag="VF058_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX6207515.1"
                     /translation="MTPDSLRVLRSGRRPSGAVLASPTFPVYRYSWLRDGAFIAEAFA
                     ASREREAAKAFHRWATGTVERYGHKVEALERGIPDAITDADVLHPRYTEEGEEVQDPL
                     WSNFQLDGHGFWLAALARHVEAGIAKPREVLDAVGLTTRYLRLLWDRPCADCWEEHDG
                     LIHPTTLCAVAAGLRAAAEILDDPEPAETGKAALARVRERGLTSGGSLAKHEGVEDVD
                     ASALLVLGPLGPFEPDDPIVGATLARIEADLVADCGGVHRYLDDGYYGGGLWVVLSGS
                     LARVHALAGNTDRAREVLAWVEATADRDGALPEQVSTHLRKPEMLSVWEAKWGPVARP
                     LLWSHAMHVLAWSALDQPEPPISS"
     gene            complement(1611..3110)
                     /locus_tag="VF058_04070"
     CDS             complement(1611..3110)
                     /locus_tag="VF058_04070"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX6207516.1"
                     /translation="MTRRLLIATIVVVLASGGLIAARSGGLRIEVPPGAGAASVLERA
                     PKGSTLVLLAGRHAPFEIDRRATVEAERGAVVSGPIVVRADGTRLEGLTIEGGETGVV
                     VRGADDIVLRGVLVEGAADHGIEVVDASARIEGCAIRGLTGAMAQGIEVRNANGRPRT
                     VISGCAVRGGMEGIVSHVSRLEAVDNVVTGTTVRGISITEMSEGLVEGNVVRDALGSG
                     VFCGDMSHCEVRRNTISGIRGDGSGITSRSGYGVVALYYATMRVHQNELVDAPNGVGT
                     LVGSVTVDRFPLSIWAQGWQGLLPGIVVVVALALAVLALARWAVTPWARRWRRRAGPV
                     DPGKALPILVGGFAVQSFHMLEHLVQVYQVHVIDAEIRSGLAGQKVDTEWVHFVYNLA
                     VLAFIVWAWRLVRPGGALAGGVGKGAGFLLAAFVVQGYHMAEHTAKLVQHLSLGIDPA
                     PGIIGGAAGLVWFHFGINLTVYVGMAIPVVSAIRTAFGGGRDLVVEPGR"
     gene            complement(3107..3946)
                     /locus_tag="VF058_04075"
     CDS             complement(3107..3946)
                     /locus_tag="VF058_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019359660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX6207517.1"
                     /translation="MGRIMHAVSRGPVAIVLAIVGLLWLVPTFGLLITSLRSNAANLE
                     SGWWNVFTVPSQLTLESYRGLLDDPTMLSGLWNTVLITVPTSLLLVMIAAGAAYAFAW
                     SRFPGRDVLFLVVVGLLVVPIQIGLIPISSLFGRLNLFGTIPGVVLYHIAFGLPFAIF
                     LLRNFFIGIPKELLEAARMDGASEWRVFTRVVLPLGIPAIASLLIFQFLWTWNDLLVA
                     LVFAGTDNAPLTVIIREQTRQFGSNIGIIAPAAFLQMIVPLAVFFAFQRYFVQGLLAG
                     STK"
BASE COUNT          637 a         1490 c         1398 g          525 t
ORIGIN      
        1 cgctcctcgc ggtccacctt cacgttgacg aagtgctcgt tcatgagcgc ggccgtgtcc
       61 tcgtcctcga acgactcccg ctccatcacg tggcaccagt ggcaggcggc gtagcccacg
      121 gagagcagga tgggccggtc ctcggcgcga gcgcgctcga gggcctccgg gccccactcg
      181 taccagtcca ccgggttgtc ggcgtgctgg agcaggtagg ggctggtggc gccctggagt
      241 cggttgggca tcgaggcgat gctacccggt ggagcatccg gcgacgcccc gctagactgc
      301 ccccgaggga ggcagcatgg ccgcggacga gttcgacgag aggttccacg aagccgtcac
      361 ggcgctcgac gagatcgcgg gcgagatcaa gccgcccgag gcctgggacg gcttcgatcc
      421 ggtgtcgctg gaggagttct ggcagtcgtg gcccgccgtc cgcgcctggg gcgagtggct
      481 gtggcagctc gtcgaagcgg agcggggcga gaaggcggcg cccgccgagg actcccccta
      541 cgaggagatc ggcggctcgg gctgatccag cgcgctccac gccaagacgt gcatcgcgtg
      601 ggaccagagg aggggcctgg ccaccggacc ccactttgcc tcccagacgg acagcatctc
      661 gggcttccgc aggtgcgtgg agacctgctc gggcagcgcg ccgtcacggt cggcggtggc
      721 ctccacccag gccaggacct cccgggcacg gtccgtgttc cccgccagcg cgtggacgcg
      781 ggccagcgac cccgacagca cgacccagag cccgccccca tagtacccgt cgtctaggta
      841 ccggtggact cctccgcagt ccgcgacgag gtcggcctcg atccgggcga gggtggcgcc
      901 gacgatcggg tcgtcgggct cgaagggccc gagcgggccc agcacgagga gcgcgctggc
      961 gtccacgtcc tccaccccct cgtgcttggc cagggagccg ccggaggtga gtccccgctc
     1021 ccgcaccctg gccagcgccg ccttccccgt ctcggcgggc tccggatcgt cgaggatctc
     1081 ggccgcggcg cgcagccccg cggccacggc gcagagagtg gtggggtgga tcaggccgtc
     1141 gtgctcctcc cagcagtcgg cgcagggccg gtcccacagg aggcggaggt agcgggtcgt
     1201 gaggccgacc gcgtccagaa cctcccgcgg cttcgcgatc ccggcctcga cgtgtcgggc
     1261 gagcgccgcg agccagaagc cgtgcccgtc gagctggaag ttcgaccaga gggggtcttg
     1321 cacctcctcc ccctcctcgg tgtagcgagg atggaggacg tccgcgtcgg tgatggcatc
     1381 ggggatgccg cgctccagcg cctcgacctt gtgcccgtac cgctcgacgg tccccgttgc
     1441 ccagcggtgg aaggccttcg ccgcctcgcg ctcgcgcgag gcggcgaagg cctccgcgat
     1501 gaaggccccg tccctcaacc acgagtaccg gtagacgggg aaggtgggcg aggcgaggac
     1561 cgcgccggac ggccggcgcc ccgatcgcag gacccggagg ctgtccggcg tcaccgaccg
     1621 ggctcgacca cgaggtcgcg cccgccgccg aaggcggtgc ggatcgccga gaccacgggg
     1681 atggccatgc ccacgtacac cgtgaggttg atcccgaagt ggaaccagac caggcccgcc
     1741 gcacccccga tgatgccggg cgcggggtcg atcccgagcg agaggtgctg gaccagcttc
     1801 gccgtgtgct ccgccatgtg gtagccctgc accacgaacg ccgccagcag gaagccggcg
     1861 cccttcccca cgccgcccgc cagcgcgccc ccgggccgca cgagcctcca cgcccagacg
     1921 atgaaggcga gcaccgccag gttgtagacg aagtgcaccc attcggtgtc gaccttctga
     1981 cccgcgagcc cgctccggat ctcggcgtcg atcacgtgca cctggtagac ctgcacgagg
     2041 tgctcgagca tgtggaagct ctgcaccgcg aagcccccga ccaggatggg cagggccttc
     2101 cccggatcga ccggcccggc ccgcctccgc cacctccgag cccacggcgt gaccgcccag
     2161 cgggccagcg cgagcaccgc gagcgccagc gccacgacca cgacgatgcc gggcagcagc
     2221 ccctgccatc cctgcgccca gatcgagagc gggaagcggt ccacggtcac cgatcccacg
     2281 agcgtcccca ccccgttcgg ggcgtcgacc agctcgttct ggtggaccct catcgtggcg
     2341 tagtagagcg ccacgacccc gtagccggag cggctcgtga tcccggaccc gtcgccccgg
     2401 atgccgctga tcgtgttccg acgcacctcg cagtgcgaca tgtcgccgca gaagaccccc
     2461 gacccaaggg cgtcgcggac cacgttcccc tccacgaggc cctcgctcat ctcggtgatc
     2521 gagatgccgc gaacggtggt ccccgtcacg acgttgtcca cggcctccag cctgctcacg
     2581 tgggagacga tgccctccat gcccccgcgg acggcgcacc ccgagatcac ggtccggggc
     2641 cgcccgttcg cgttccgaac ctcgatgccc tgggccatcg ctcccgtgag ccctcggatc
     2701 gcgcagccct cgatccgagc cgaggcgtcc accacctcga tcccgtggtc ggcggcgccc
     2761 tcgacgagga cgcccctcag cacgatgtca tcggcgcccc gcacgacgac gcccgtctcg
     2821 ccgccctcga tcgtgaggcc ctcgaggcgc gtgccgtccg cccgcaccac gatcggcccc
     2881 gacaccacgg cgcctctctc cgcctccacg gtggctcgcc ggtcgatctc gaacggggcg
     2941 tgtcgcccgg cgagcaggac gagcgtcgac cccttcgggg cgcgctcgag caccgaggcg
     3001 gcgccggcgc cgggcggcac ctcgatccgc agccccccgg agcgggccgc gatcaggccg
     3061 ccgctggcca ggacgacgac gatcgtggcg atcagcaggc gcctggtcac ttggtcgagc
     3121 cggccagcag gccctggacg aagtaccgct ggaacgcgaa gaacacggcc aacggcacga
     3181 tcatctggag gaacgcggcg ggggcgatga tcccgatgtt cgatccgaac tgccgcgtct
     3241 gctccctgat gatcaccgtc agtggggcgt tgtcggtccc cgcgaacacg agcgcgacca
     3301 gcaggtcgtt ccacgtccac aggaactgga agatcagcag ggaggcgatc gccgggatcc
     3361 cgaggggcag caccacgcgc gtgaagaccc gccactcgct ggccccgtcc atccgcgccg
     3421 cctcgagcag ctccttgggg atcccgatga agaagttgcg tagcaggaag atggcgaagg
     3481 gcaacccgaa ggcgatgtgg tagaggacca ccccggggat cgtcccgaag aggttcagcc
     3541 gcccgaagag gctggagatg gggatcagcc cgatctggat cgggaccacc agcagcccga
     3601 cgaccacgag gaagagcacg tcgcgcccgg ggaagcgcga ccacgcgaac gcgtacgcgg
     3661 cgcccgctgc gatcatcacg agcagaagcg acgtggggac ggtgatcagg accgtgttcc
     3721 acaggccgct gagcattgtg gggtcgtcga ggagcccgcg gtaggactcc agcgtgagct
     3781 ggctcggcac tgtgaacacg ttccaccagc cgctctccag gttcgccgcg ttggagcgga
     3841 gcgaggtgat cagcagcccg aacgtgggca ccagccacag gagccccacg atcgcgagca
     3901 cgatcgccac cggcccgcgc gagaccgcgt gcatgatcct tcccatcggg gtcgagggcc
     3961 gggagggcgc ccctaccgtc acgtccgtcg ccatgtcact gctccgactt gaatcgccgt
     4021 aggttgaaga gcatcgccgg gaccaccagc
//
LOCUS       DASUTW010000124        12813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1464256, whole genome shotgun sequence.
ACCESSION   DASUTW010000124 DASUTW010000000
VERSION     DASUTW010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12813
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464256"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VF058_04080"
     CDS             complement(<1..609)
                     /locus_tag="VF058_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6207518.1"
                     /translation="MRVDHQEGGDHMRGKFTLLALVTVVALTAAACAEEAEEPEAAPT
                     GEEALPGEGMLACQVTDTGGIDDRSFNQTAWAGMQRAEEELGVEIDFLESQSQADYEP
                     NIQAFIDRDCDIIVTVGFLLGDATAAAAEANPEQSFAIVDFAYDPPLDNVLGLVFSTK
                     EAAFMAGYAAASWTKTGTMGTYGGINIGPPVTDFMDGFFAGAM"
     gene            701..1324
                     /gene="upp"
                     /locus_tag="VF058_04085"
     CDS             701..1324
                     /gene="upp"
                     /locus_tag="VF058_04085"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEX6207519.1"
                     /translation="MLTVVDHPLAGHLLTELRSRDTESPVFRTLTRRLTTALVLEATR
                     DLPSEPREVVTPLEEMEGSRLGARLVAVPVLRAGLGMLDSVTELFPGISVGYIGLERD
                     ERTLRPTSYYEKLPAMDGRWAVLLDPMLATGGTAAHACAAIKMRRPAEVRMVSIVAAP
                     EGVETMEEAHPDVHIFTAALDRQLNDVGYILPGLGDFGDRLYDTPPD"
     gene            complement(1303..1809)
                     /locus_tag="VF058_04090"
     CDS             complement(1303..1809)
                     /locus_tag="VF058_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX6207520.1"
                     /translation="MDVEVVRSARRRKTISAEQRGSTIVVRVPAGLPAEEERAWVSRM
                     VHRLTARERTRRLNAAGALARTADRLNRRHFDGRLRWSSIRYVPNQRSRFGSCTPQDG
                     TIRISDRVASLPAWVRDYVVMHELTHLVEADHSPGFWKLVERYPKAERARGYLMALGP
                     EDQSGGVS"
     gene            complement(1826..2209)
                     /locus_tag="VF058_04095"
     CDS             complement(1826..2209)
                     /locus_tag="VF058_04095"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX6207521.1"
                     /translation="MALKFLSEEWAQQVTEKLNASEQFSQAAGGQNAKLQQVVSTPDG
                     ETKYYFKIEDGRAEVALGELDGAETTITQDYDTAVAMSKNELSPTAAYMSGKIKVSGD
                     LMRLMQLQGVIGALPGVLAELDVEY"
     gene            complement(2278..2700)
                     /locus_tag="VF058_04100"
     CDS             complement(2278..2700)
                     /locus_tag="VF058_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX6207522.1"
                     /translation="MSEAHPTHVDVLRRVPLFADLDDEALGDVAGKAAEVEVPRGHVL
                     LERGQEGAGLFVVLSGTAEVLVGESRIEAGEGEILGELSLLVPGLRHTARVQAGEGFR
                     ALAVRRDDFESLLEDHPRMAVAMLRVVAERLAETLSYL"
     gene            complement(2731..3186)
                     /locus_tag="VF058_04105"
     CDS             complement(2731..3186)
                     /locus_tag="VF058_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207523.1"
                     /translation="MLRSERGSGSVLTIATILLGAALAIGFPVWMLIRGTQMQAESAA
                     VGVQQAEDLRADALLTGAAGVAHSYLATQGSFQGFSPQAAAALDPSFRWNADPVASSG
                     TVSIRGVTATSVALVTVGSGRPLCLAIDSGQTRRGTTDARSAADCTGGS"
     gene            complement(3226..3597)
                     /locus_tag="VF058_04110"
     CDS             complement(3226..3597)
                     /locus_tag="VF058_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207524.1"
                     /translation="MEIAVALRRENGGWTALASDPPVSVRAESREDCVRRVRDAVLAR
                     RPNGSDPVTVVVEVAPDLVGVAEAASILGWDKRRVITYVDRGSFPEPLARLAGGRVWA
                     RADVEAFRLAFLERQARRRRR"
     gene            3666..4724
                     /locus_tag="VF058_04115"
     CDS             3666..4724
                     /locus_tag="VF058_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2578c family radical SAM protein"
                     /protein_id="HEX6207525.1"
                     /translation="MRWDNLRLETGPPDDRLTAPLFEQGAVARTFDTPEFRGMTFYEV
                     HARSIINRVPEASRVPFRYTINPYRGCSHACVYCFARKTHTYLDLDHGADFDTRIVVK
                     VNAPQLLRKELSTRRWSGEHIAMGTNVDPYQRAEGRYRLMPGIIEALRDFANPFSILT
                     KGTLILRDLDLLREASEVTEVGTNFSVGFVDDELSRLVEPGTPRPASRLEAVRKLNEA
                     GIPCGVLMAPILPFLTDDDEHLEATVAAVAGAGATHVSPIVLHLRPGAREWWLAWIER
                     ERPDLLPRYRDLYGRGAYAPKEYQAEISAMVRELARRHGVGRGNPREAGNPRRIPEEP
                     PQPEQLTLEAQLESRSAR"
     gene            complement(4697..5725)
                     /locus_tag="VF058_04120"
     CDS             complement(4697..5725)
                     /locus_tag="VF058_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013016236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX6207526.1"
                     /translation="MRPWSEEPVGRFEERTIESEALKGNPLGDPHERPIWVYLPPGYD
                     DEPQRRYPTIYEIQGYTGQLDMWRNRSPWRRTFPELLDRMYAGADAPPPAIVVFVDCW
                     TSLGGSQFLDSPGTGRYHTYLCEEVVPWVDAEYRTLAGRDHRGIAGKSSGGYGAMVTP
                     MLRPEVFGGFATHAGDALFEVCYCPEFREAARYLRDHYAGSYEAFLEDFRSRPAFSKK
                     QDGTLIELYGYAACYSADEDGTVRLPFDTDTGRLIPEVWARWLAWDPVRMVSERADAL
                     RSMRAIYVDAGKRDEPYLDLAAEAFRRELEAIGVTEYFFEIFDAGHMGIEYRYPLSVR
                     YLAERLSS"
     gene            complement(5722..5985)
                     /locus_tag="VF058_04125"
     CDS             complement(5722..5985)
                     /locus_tag="VF058_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014144921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207527.1"
                     /translation="MPRRHRRDRTGPEPLRPIGAVTPPGEHVEGWSVRFSTKGGATCP
                     YCNRPIPDGQQHVVAWPEDQEDYRRHWHRGCWNREKRRGGPTL"
     gene            complement(5985..6623)
                     /locus_tag="VF058_04130"
     CDS             complement(5985..6623)
                     /locus_tag="VF058_04130"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX6207528.1"
                     /translation="MTRTIETPKGPVSALLEGPEGGPLLVLAHGAGNDMRTPLFDGLA
                     QGLASRGVATLRFNFPYKEQGRRAPDPERTLRDAWLAAFEHAAALSDVVWVGGKSLGG
                     RIASLVVTEGLAAAGIVFVGYPLHPPGKPERIRDAHLPGIEVPMLFLQGTKDPFARWE
                     LLEPLVERLPDATLHPVDGGDHSFRVRGQPKDDAATGRALGEVAGDFIRERS"
     gene            6672..6926
                     /locus_tag="VF058_04135"
     CDS             6672..6926
                     /locus_tag="VF058_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002787429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207529.1"
                     /translation="MDTLTATVNALFAAAVVVILGWIVRGGFRDVHRRIDDLERRFTD
                     RFEQVDKRFEVVEERLHQIRSDISALAFRLVGPPRAEGER"
     gene            complement(6927..9389)
                     /locus_tag="VF058_04140"
     CDS             complement(6927..9389)
                     /locus_tag="VF058_04140"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX6207530.1"
                     /translation="MAPTRSDRAAGPIDRLQRGLRSLEEAYSSGGHGAWSARRRTELV
                     DRAVRELAEGLEGRVAVVALGGYGRGELTPGSDVDLMLLHEGDGELEEAASRLFYPFW
                     DAGISLGHSVRTVAECEKLVGDLDVATSLLDARLVAGDGSVLEALTEGLLARMGLGRR
                     AFLASIAESVEARRERLGSCAWLLEPDLKASGGGAWDVAAVGWMLKGLRVEDAVRAGL
                     LRAREVEGLDEAREFLIRLRSALHLETGRRGDRLLLEHQPALAEAFGFGPEPGMTPAD
                     VLMRSLFEHARSVEHILRLLLDRAARWLEGAEEEEEAGPGSPEEVLDQLALAAERERL
                     PPPATLDRLERTVPEGATWTPAMREAFVRLLAAPGAVEALEAMDRGGILVRLLPEWAA
                     VRCRPQRDPYHRFTVDLHLIHTAAGAAALLRGGAEDPMTAEAVFSIRDREALLLGALL
                     HDIGKRGEGPHVPAGVRVLDPILERLGVAPETADRVRFLVGEHLLLSDTATRRDLTDE
                     NLVLDVAGRVLDGERLASLFLLTVADAEATGPHAATPWRRTLVRDLVGKVQRALDRPD
                     EQPDELATIAERLERTAALLAGEHPDAVGAYVDRLPRPYVLAVPPETAARHFRLLEPP
                     PAAGEIRTDVLEGERPGVHDLAVAAADRRGLLADIAGALALQGLTILSARAFTSADGI
                     ALDLFAVQGVFDPEITEERWRRFRGDLRRALEGRVSLERRVEEKRRHYPPPAPVEPEV
                     RVEDRVSEFSTVVEVSAGDRLGLLFDLSLAFRDLGLTVHVAKVATYGPRVVDVFYVRD
                     LEGRKVGERAEDIREAVLARLG"
     gene            complement(9370..9708)
                     /locus_tag="VF058_04145"
     CDS             complement(9370..9708)
                     /locus_tag="VF058_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016654695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX6207531.1"
                     /translation="MKLVTAIIKPHKLDEVKDALREIGVEGMTATEAQGFGRQRGHTE
                     IYRGAEYQVDFVPKVKIEIVTEDARAPEVVETIERSARTGKIGDGKIFVTEAEQVVRI
                     RTGERGPDAL"
     gene            complement(9736..10578)
                     /locus_tag="VF058_04150"
     CDS             complement(9736..10578)
                     /locus_tag="VF058_04150"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX6207532.1"
                     /translation="MRWDLLPDISAFDSARRKEAYRRLARSVRGGREAPTLLDLDEVR
                     TRLGVFEESYEGIRAIPVSRIVGTAGRSRDFDGDFLPRDERIRERWRKVEQTFPRGDF
                     PPIVAYEVDGRYFVVDGHHRVAVAKQRGVDYLDAEVTRLRTRRPLPEGADVGALIIAE
                     QERRFLEEAGLDDLDPPVHIPMSSPVGYPQLLELVKVHGFHLSGERGEVVSLPEAGRD
                     WYERVCLPAMEAAGETGLRDALPGAREGDVFLWVYDRHRALRPEHPEAGLAEAARRIL
                     EERR"
     gene            complement(10590..11411)
                     /locus_tag="VF058_04155"
     CDS             complement(10590..11411)
                     /locus_tag="VF058_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX6207533.1"
                     /translation="MPRTMWKGAISFGLVSIPVRVFPATEEKTLRFNQLHDEDHGRIR
                     YQRVCAKCGKEVPYENIVKGYEYEKDRYVVLTDDEIDQIPVKSTRAIDITQFVDIDEI
                     DPVYFKKTYYLVPEETGIKAYQLMREALREDDKVGIAKVSFRDKEHLAALRLMGDVIV
                     LDTMYWPDELRAQDFEELDAKSEVRPKEVQMARSLIENLTEPWNPEQYGDEYREALLE
                     IVEKKVAGEEIEVITEEEAPEKVVDLMEALQKSVEATKTEKKPARKRAAKKQAAS"
     gene            11494..>12813
                     /gene="ligD"
                     /locus_tag="VF058_04160"
     CDS             11494..>12813
                     /gene="ligD"
                     /locus_tag="VF058_04160"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX6207534.1"
                     /translation="MARGEPFTVETPQRLEAEREGDHWWVEAAGRRVRLSNLPKVYWP
                     DDGYTKGDLLAYYWNVADTILPYLRGRPLTMRRMPDGIEGGEFYEKNAPSHTPEWMPR
                     CPVLDEEGSKTIEYLRVEDEAGLLFVANLGAIEFHPLHSRCDSVDRPDYLFFDLDPFE
                     PITFDDVRAVARHVGAALDALGLPSYPKTSGATGMQIYVPIEPGPSYQQVRDFVGAVG
                     RLILKADADRVTMEWEVSKRAGKVFIDHNMNRQGANIAAAYSVRPEPGATVSAPVTWQ
                     EVEEGIEPGDFTIITMHERLAEVGDLFRGVLEEPVDLRPALEALGMDANVEEAEAPTA
                     AESEPVSEELTADPKADDGDAAARLAKYGEMRDFAETPEPPPVPAGGDGRSVVIQKHE
                     ATRLHYDLRLEEGGVLISWAVPRGLPLTPGDRRLAVHTEDHPLDYGGF"
BASE COUNT         1832 a         4765 c         4391 g         1825 t
ORIGIN      
        1 catcgccccg gcgaagaacc cgtccatgaa gtcggtgacg ggcgggccga tgttgatccc
       61 gccgtaggtg cccatcgtgc ccgtcttggt ccacgaggcc gcggcgtagc cggccatgaa
      121 cgccgcctcc ttcgtggaga agaccagccc caggacgttg tccagcggcg ggtcgtaggc
      181 gaagtcgacg atcgcgaagc tctgctccgg gttggcctcg gcggcagccg ccgtggcgtc
      241 cccgagcagg aagccgaccg tcacgatgat gtcgcagtca cggtcgatga acgcctggat
      301 gttcggctcg tagtcggcct gcgactgtga ctccaggaag tcgatctcga cccccagctc
      361 ctcctcggcc cgctgcatgc ccgcccaggc ggtctggttg aacgacctgt cgtcgatgcc
      421 gcccgtgtcg gtcacctgac aggcgagcat gccctcgccc ggaagggcct cttccccggt
      481 cggggcggct tcgggctcct cggcttcctc ggcacacgcc gcggcggtga gcgctacgac
      541 ggtgacgaga gcgagaagcg tgaacttccc acgcatgtga tctcccccct cctggtggtc
      601 tacccgcaca gcggttcgga ttgtacgcca gtgccacccg gcgggtacag gaccgagggc
      661 ggtgcgcgga agggccgtca gcccctagga tgtgatcgcc atgctcacgg tcgtcgatca
      721 cccgctggcc ggccacctgc tgaccgagct ccgcagccgc gacaccgagt cgccggtctt
      781 tcggacgctg acccgtcgcc tgaccaccgc gctcgtcctg gaggccacgc gcgacctccc
      841 cagcgagccc agggaggtcg tgaccccgct ggaggagatg gaggggagcc ggctcggggc
      901 ccggctcgtg gccgtgccgg tcctgcgggc gggcctgggc atgctcgact cggtcacgga
      961 gctcttcccg gggatctcgg tcggctacat cggcctcgag cgcgacgagc ggaccctccg
     1021 gccgacctcc tactacgaga agctgccggc catggacggg cggtgggcgg tcctcctcga
     1081 tccgatgctg gccacgggcg gcaccgccgc ccacgcgtgc gcggcgatca agatgcgtcg
     1141 cccggcggag gtccggatgg tctccatcgt cgccgccccc gagggggtgg agacgatgga
     1201 ggaggcccac cccgacgtgc acatcttcac cgcagcgctg gatcgccagc tgaacgacgt
     1261 gggctacatc ctgccgggtc tcggggactt cggcgaccgg ctctacgaca ccccgcccga
     1321 ctgatcctcc ggtcccaggg ccatcaggta cccacgggcc cgctcggcct tcgggtaccg
     1381 ctccacgagc ttccagaacc cgggcgagtg gtcggcctcg acgagatgcg tgagctcatg
     1441 catcacgacg tagtcgcgga cccacgcggg cagcgacgcg acgcgatcgg agatgcggat
     1501 cgtgccgtcc tggggggtgc acgacccgaa gcggctgcgc tgattcggca cgtagcggat
     1561 cgaggaccac cggaggcggc cgtcgaagtg gcgccggttg aggcggtcgg cggtgcgcgc
     1621 gagcgcaccc gccgcgttga ggcggcgggt gcgctcgcgc gccgtgagcc ggtggaccat
     1681 ccggctcacc cacgcccgct cctcctcggc cggcaggccc gcggggacac gcacgacgat
     1741 ggtgcttccc cgctgctcgg ccgagatcgt ctttcgccgc ctcgcgctgc gaacgacctc
     1801 gacgtccacg cggacctggg cgggatcagt actcgacgtc gagctccgcg agcacgccgg
     1861 gcagggcgcc gatgacccct tggagctgca tcagcctcat caggtccccg gagaccttga
     1921 tcttgccgct catgtaggcg gccgtggggc tgagctcgtt cttcgacatg gccacggcgg
     1981 tgtcgtagtc ctgcgtgatg gtggtctccg cgccgtcgag ctccccgagg gcgacctcgg
     2041 ccctcccgtc ctcgatcttg aagtagtact tcgtctcgcc gtccggcgtg ctcaccacct
     2101 gctgcagctt cgcgttctga ccgcccgcgg cctggctgaa ctgctcgctg gcgttgagct
     2161 tctccgtgac ctgctgggcc cactcctccg agaggaactt caaagccacg cttgcctcct
     2221 tgttcctacc gctgggtagc catcatccca aagggccgga ccctcggtca atcgagatca
     2281 caggtacgag agggtctccg ccaggcgctc ggccactacc cggagcatcg ccacggccat
     2341 ccgcgggtgg tcctcgagca gcgactcgaa gtcgtctcgg cgcaccgcga gggcccgaaa
     2401 gccctcgccc gcctgcaccc gggccgtgtg gcggagaccg gggacgagga gcgacagctc
     2461 cccgaggatc tcgccctccc ccgcctcgat ccgggattcg cccacgagga cctcggccgt
     2521 cccgctcagc acgacgaaga gccccgcacc ctcctgtccc cgctccagca ggacgtgccc
     2581 ccgcggcacc tcgacctcgg cggccttccc cgcgacgtcg ccgagcgcct cgtcgtcgag
     2641 atcggcgaac agcggaacgc ggcgaaggac gtccacgtgc gtgggatggg cctcgctcat
     2701 gcgcgaaaga ctacacggcg gacgcggcgg tcaggacccg ccggtgcagt cggcggccga
     2761 gcgggcgtcg gtcgtccctc tgcgggtctg acccgagtcg atcgcgaggc agagcggccg
     2821 acccgaaccc acggtcacca gcgcgaccga ggtggcggtc acgccccgga tgctgaccgt
     2881 cccggacgac gcgaccgggt ccgcgttcca gcggaacgag ggatcgagcg ccgccgccgc
     2941 ctgcggtgaa aaaccctgga acgagccctg cgtcgcgaga tacgagtggg ccaccccggc
     3001 ggcgccggtc aggagggcgt ccgcccgcag atcctcggcc tgctgcaccc cgacggccgc
     3061 gctctcggcc tgcatctggg tcccccggat cagcatccac acggggaacc cgatcgccag
     3121 cgccgccccc aggaggatcg tggcgatcgt caggacgctg ccgctcccgc gctccgaccg
     3181 aagcatgccc caggtatcga ccctctgggc ccggggctga agggatcagc ggcgccggcg
     3241 ccgggcctgc cgctcgagga aggcaaggcg gaacgcctcg acgtcggccc gggcccaaac
     3301 gcggcctcca gcgaggcggg ccaggggctc cgggaaggag ccccggtcga cgtaggtgat
     3361 cacccgacgc ttgtcccagc ccaggatcga ggcggcctcg gccacgccca ccaggtcggg
     3421 ggccacctcg acgaccaccg tgaccgggtc gctgccgttg gggcggcggg ccagcaccgc
     3481 gtcccggacc cgacgcacgc agtcctcgcg ggactcggcc cggacggaga ccggcgggtc
     3541 ggaggccagg gccgtccagc ctccgttttc cctgcggagc gccaccgcga tctccacgac
     3601 gacggcatca tacgatcgtc gggacgcttg cccgaaccga acacacgttc tatcctggcc
     3661 gtcccatgcg gtgggacaac ctccgactcg agaccggtcc cccggacgac cgcctcacgg
     3721 cgccgctctt cgagcagggg gcggtggcca ggaccttcga cacgcccgag ttccgcggga
     3781 tgaccttcta cgaggtgcac gcccgctcga tcatcaaccg ggtgcccgag gcctcccgcg
     3841 tcccattccg ctacacgatc aacccgtaca ggggatgcag ccatgcgtgc gtttactgct
     3901 tcgctcggaa gacgcacacg tacctggacc tcgaccacgg cgcggacttc gacacgagga
     3961 tcgtggtgaa ggtcaacgcg ccccagctcc ttcgcaagga gctgtcgacc cggcgctggt
     4021 cgggcgagca catcgcgatg gggacgaacg tggatcccta tcagagggca gaggggcggt
     4081 atcggctgat gccgggaatc atcgaggcgc tgcgggactt cgccaacccg ttctcgatcc
     4141 tcacgaaggg gacgctgatc ctgcgcgacc tcgacctgct gcgggaggca tccgaggtca
     4201 ccgaggtggg gacgaacttc tcggtgggct tcgtggacga tgagctgtcg aggctggtcg
     4261 agccggggac gccgcgcccc gcgtcgcgcc tggaggcggt gcggaagctg aacgaggccg
     4321 ggatcccctg cggggtgctg atggcgccga tcctcccgtt cctcaccgac gacgacgagc
     4381 atctggaggc cacggttgcc gcggtcgcgg gcgccggggc gacgcacgtg tcaccgatcg
     4441 tcctccatct gcgccccggc gcccgcgaat ggtggctggc ctggatcgag cgcgagcgtc
     4501 ccgacctcct cccccgctac cgggacctgt acggacgggg cgcctacgcc cccaaggagt
     4561 accaggcgga gatctcggcc atggtgcggg agctcgcccg gcgccacggc gtcgggaggg
     4621 gcaacccacg ggaggcgggg aacccgagga ggatccccga ggagccgccg cagccggagc
     4681 agctcacact cgaggctcag ctcgagagcc gctcggccag atagcgcacc gacagggggt
     4741 agcggtactc gatccccatg tggccggcgt cgaagatctc aaagaagtac tcggtcacgc
     4801 cgatcgcctc cagctcccgc cggaacgcct cggcggcgag gtccaggtac ggctcgtctc
     4861 gcttccccgc gtcgacgtag atggcgcgca tcgaccgcag cgcgtcggcc cgctcggaga
     4921 ccatccggac cgggtcccag gccagccacc gggcccagac ctcggggatc aggcgccccg
     4981 tgtcggtgtc gaacggcagc cgcacggtgc cgtcctcgtc cgccgagtag caggccgcgt
     5041 acccgtacag ctcgatcagc gtcccgtcct gcttcttgga gaaggccgga cgggagcgga
     5101 agtcctcgag gaaggcctcg taggagccgg cgtagtggtc gcgcaggtat cgggcggcct
     5161 cccggaactc cgggcagtag cagacctcga acagcgcgtc gccggcatgg gtggcgaagc
     5221 ccccgaacac ctccggcctg agcatggggg tcaccatcgc cccgtatccg ccgctcgact
     5281 tccccgcgat gccccggtgg tcgcggcccg ccagggtccg gtactccgcg tccacccacg
     5341 ggacgacctc ctcgcagagg taggtgtggt agcggccggt ccccggcgag tccaggaact
     5401 ggctcccccc gagcgacgtc cagcagtcca cgaacacgac gatcgcgggc ggaggcgcgt
     5461 cggcccccgc gtacatccgg tcgagcagct cggggaaggt ccggcgccac ggggagcggt
     5521 tccgccacat gtccagctgg cccgtgtagc cctggatctc gtagatcgtc gggtagcggc
     5581 gctgcggttc gtcatcgtag ccgggcggga ggtacaccca gatcgggcgc tcgtgcggat
     5641 cgccgagcgg gttgcccttc agcgcctcgc tctcgatcgt tcgctcctcg aaccgcccca
     5701 cgggctcctc cgaccacggc ctcacagcgt cgggcctcct ctccgcttct cccggttcca
     5761 gcacccccgg tgccagtgcc gtctgtagtc ctcctggtcc tcgggccagg ccacgacgtg
     5821 ctgctgcccg tccgggatcg gtcggttgca gtaggggcag gtggcgccgc ccttggtcga
     5881 gaaccggacg ctccacccct cgacgtgctc ccccggcggc gtgacggcgc cgatcggtcg
     5941 aagcggctcg ggacccgtcc gatcgcgccg gtggcggcgc ggcatcagct ccgctccctg
     6001 atgaagtccc cggcgacctc accgagggct cgtccggtcg ccgcgtcgtc cttgggctgc
     6061 ccccggacgc ggaaggagtg gtctccgccg tccaccgggt gcagcgtcgc gtcggggagc
     6121 cgctccacca gcggctccag cagctcccac cgcgcgaagg ggtccttcgt cccctggagg
     6181 aacagcatcg ggacctcgat cccggggagg tgggcgtcgc ggatccgctc cggcttgccc
     6241 ggagggtgca gggggtagcc gacgaacacg ataccggcgg cggcgagccc ttcggtcacc
     6301 acgagcgagg cgatccggcc cccgagcgac ttgcctccca cccagacgac gtcgctgagc
     6361 gcggccgcgt gctcgaaggc ggccagccac gcgtcccgga gcgtgcgctc cgggtccggg
     6421 gcccggcggc cctgctcctt gtaggggaag ttgaagcgga gcgtcgccac cccgcgggag
     6481 gcgagcccct gggccaggcc gtcgaagagc ggggtgcgca tgtcgttccc ggcgccgtgg
     6541 gccaggacga ggagcgggcc cccctcgggc ccctcgagca gggccgagac cggccccttc
     6601 ggcgtctcga tcgttcgcgt caccccgcga tggtagcctg agcccggcat cctcccccgg
     6661 gaaggagctg catggacacg ctgacggcca ccgtgaatgc gctcttcgcc gcggccgtcg
     6721 tcgtcatcct ggggtggatc gtccggggcg ggtttcggga cgtccaccgt cgcatcgacg
     6781 acctcgagag gcgcttcacc gaccggttcg agcaggtgga caagcggttc gaggtggtcg
     6841 aggaacggct ccaccagatc cggtccgaca tctcggcgtt ggcattccgc ctcgtgggcc
     6901 ccccgagggc cgaaggagag cgctagctag ccgagccggg cgaggacggc ctcgcggata
     6961 tcttccgccc gctcccccac cttccgcccc tcgaggtccc gcacgtagaa gacgtccacg
     7021 acgcgcggac cgtaggtggc caccttcgcc acgtgcacgg tgagccccag gtcccggaag
     7081 gcgagcgaga ggtcgaagag gaggccgagg cggtcgccgg ccgacacctc gaccacggtg
     7141 gagaactccg agactcgatc ctccacccgg acctccggct ccaccggcgc cggcggtggg
     7201 tagtggcgcc gcttctcctc caccctccgc tccagcgaga cccggccctc gagggcgcga
     7261 cgcagatcgc ctcggaaccg ccgccaccgc tcctcggtga tctcgggatc gaagacgccc
     7321 tgcacggcga agaggtcgag ggcgatcccg tccgcgctcg tgaaggcccg ggccgagagg
     7381 atggtcaggc cctggagggc gagcgccccg gcgatgtctg ccaacagccc ccgtcggtcc
     7441 gccgccgcga cggccaggtc gtggacgccg gggcgctcgc cctccaggac gtcggtccgg
     7501 atctcgcccg ccgccggcgg aggctccagg aggcggaagt ggcgggccgc cgtctcggga
     7561 ggcaccgcga ggacataggg gcgggggagg cggtcgacgt aggcgcccac cgcatcggga
     7621 tgctcgcccg ccagcagggc cgccgtccgc tccagccgct ccgcgatcgt cgccagctcg
     7681 tccggctgct cgtcgggacg gtcgagcgcc cgctggacct tccccacgag atcgcgcacg
     7741 agggtccggc gccagggcgt cgcggcgtgg ggaccggtgg cctccgcgtc cgcgacggtc
     7801 aagaggaaga gcgacgccag gcgctccccg tccaggaccc tgccggccac gtcgaggacg
     7861 aggttctcgt cggtgaggtc gcgccgcgtc gcggtgtccg agaggaggag gtgctccccg
     7921 accaggaagc gcacccggtc cgcggtctcc ggcgcgacgc cgagccgctc caggatcgga
     7981 tccaacacac ggaccccggc cgggacgtgc ggcccctctc cccgcttgcc gatgtcgtgc
     8041 agcagcgcgc ccagcaacag ggcctcgcga tcccggatgg aaaagaccgc ctcggccgtc
     8101 atcggatcct cggccccgcc ccgcagcagc gccgcggcgc ccgcagccgt atggatcagg
     8161 tggaggtcca cggtgaaccg gtggtaggga tcgcgctgcg ggcggcaccg gaccgccgcc
     8221 cactccggca ggagccgcac caggatcccg ccgcggtcca tcgcctccag ggcttcgacc
     8281 gcaccgggcg ccgccagcag gcggacgaag gcctcgcgca tcgccggcgt ccacgtcgcc
     8341 ccttcgggga cggtccgctc cagccggtcg agcgtggccg gcggcgggag cctctctcgc
     8401 tccgcggcca gcgccagctg gtcaagcacc tcctcggggg agccaggccc ggcctcctcc
     8461 tcttcctcgg ccccctccag ccagcgggcg gcccggtcga gcaggagccg gaggatgtgc
     8521 tccacggatc gggcgtgctc gaacagggat cgcatcagca cgtctgcggg cgtcatcccc
     8581 ggctcgggcc cgaagccgaa cgcctcggcg agcgccggct ggtgctcgag gagcagacgg
     8641 tcgccgcgcc gccccgtctc caggtgcagg gcgctccgga ggcggatcag gaactcgcgg
     8701 gcctcgtcca ggccctcgac ctcgcgggcc cgcagcaggc ccgcccggac cgcgtcctcc
     8761 acccggagcc ccttcagcat ccaccccacc gccgccacgt cccacgcgcc gcccccgctc
     8821 gccttgaggt ccggctcgag cagccatgcg cacgagccca gccgctcgcg acgggcctcc
     8881 acgctctcgg cgatcgaggc caggaaggcc ctccggccca gccccatcct cgccagcagc
     8941 ccttcggtga gggcctcgag caccgaccca tcccccgcca cgagcctcgc gtcgaggagc
     9001 gacgtggcga cgtcgaggtc cccgaccagc ttctcgcact ccgccacggt ccggaccgag
     9061 tgccccagcg agatccccgc gtcccagaac ggatagaaga ggcgagacgc cgcctcctcg
     9121 agctccccgt cgccctcgtg cagcagcatg aggtcgacat ccgagcccgg ggtcaactcc
     9181 cctcgaccgt agcccccgag cgccaccacg gccacgcgcc cctcgaggcc ctccgccagc
     9241 tcacggacgg cgcggtccac cagctcggtc cggcggcgcg ccgaccaggc gccgtggccc
     9301 ccgctcgaat acgcctcctc cagcgagcgc aaccccctct ggagccggtc gatcgggccg
     9361 gccgcccgat cagagcgcgt cggggccacg ctccccggtg cggatgcgga ccacctgctc
     9421 ggcctcggtg acgaagatct tcccgtcgcc gatcttcccg gtccgcgccg accgctcgat
     9481 cgtctccacg acctcggggg cccgggcgtc ctcggtcacg atctcgatct tgaccttggg
     9541 gacgaagtcg acctggtact cggccccgcg atagatctcg gtgtggccgc gctggcggcc
     9601 gaagccctgg gcctcggtgg ccgtcatgcc ctcgaccccg atctcgcgca aggcgtcctt
     9661 cacctcgtcg agcttgtgcg gcttgatgat cgccgtcacc agcttcatgc gtccctcctc
     9721 gcgggcccga gcatcctacc gccgctcctc gaggatccgg cgtgccgcct ccgcgagccc
     9781 ggcctccgga tgctcgggcc gcagcgcccg gtgacgatcg tagacccaca ggaagacgtc
     9841 gccctcccgg gccccgggca gggcgtctcg cagtcccgtc tcccccgcgg cctccatcgc
     9901 cgggaggcag acgcgctcgt accagtcgcg accggcctcc ggcagcgaca cgacctcccc
     9961 tcgctcgccg gagaggtgga agccgtggac cttgaccagc tcgagcagct gcgggtaccc
    10021 gaccgggctc gacatcggga tgtgcacggg cggatcgagg tcgtcgagcc ccgcctcctc
    10081 caggaaccgc cgctcctgct ccgcgatgat cagcgcgccc acgtccgcgc cctccggcag
    10141 cgggcggcgg gtccggaggc gcgtcacctc cgcgtccagg tagtccacgc cgcgctgttt
    10201 cgccacggcc acacggtggt gaccgtccac gacgaagtac cgcccgtcca cctcgtaggc
    10261 gacgatcggg gggaagtcgc cccgcgggaa cgtctgctcg accttccgcc accgctcgcg
    10321 gatgcgctcg tcgcgcggga ggaagtcgcc gtcgaagtca cgggaccgtc cggcggtgcc
    10381 gacgatgcgc gacaccggga tggctcggat cccctcgtac gactcctcga agactccgag
    10441 ccgcgtgcgg acctcgtcca ggtcgagcag cgtgggggcc tcacgccccc cgcggacgct
    10501 cctcgccaga cgccggtagg cctccttgcg cctcgcgctg tcgaaggcgc tgatgtcggg
    10561 caggaggtcc caccgcacgg ccgggcctct caggaggcgg cttgcttctt cgccgcgcgc
    10621 ttcctcgccg gcttcttctc ggtcttcgtg gcttccacgc tcttctggag cgcctccatg
    10681 agatcgacga ccttctccgg cgcctcctcc tcggtgatga cctcgatctc ctcgccggcc
    10741 accttcttct ccacgatctc gagaagcgcc tcgcggtact cgtcgccgta ctgctcgggg
    10801 ttccagggct cggtgaggtt ctcgatcagg gagcgggcca tctggacctc cttgggccgc
    10861 acctcgctct tggcgtcgag ctcctcgaag tcctgcgccc ggagctcgtc gggccagtac
    10921 atcgtgtcga gcacgatcac gtcgcccatt agccggaggg cggccaggtg ctccttgtcg
    10981 cggaacgaga ccttggcgat ccccaccttg tcgtcctcgc ggagcgcctc ccgcatcagc
    11041 tggtaggcct tgatgccggt ctcctcgggc acgaggtagt aggtcttctt gaagtagacc
    11101 gggtcgatct cgtcgatgtc cacgaactgc gtgatgtcga tggcgcgcgt ggacttcacc
    11161 gggatctggt cgatctcgtc gtcggtgagc acgacgtagc ggtccttctc gtactcgtag
    11221 cccttgacga tgttctcgta ggggacctcc ttgccgcact tggcgcagac ccgctggtag
    11281 cggatgcgcc cgtggtcctc gtcgtgcagc tggttgaagc ggagggtctt ctcctcggtc
    11341 gccgggaaca cccgcacggg gatcgacacg aggccgaagg agatggcccc cttccacatg
    11401 gtcctcggca tcacgacctc cctgttctcg gcttcgccgg cccaggatac ccggcgagcg
    11461 gccgggatgg cggccaggcc ctaccatcgg ggcatggcgc gcggcgagcc cttcaccgta
    11521 gagactcccc agaggctcga agctgagcgg gagggagacc actggtgggt ggaggcggcc
    11581 ggccgccgcg tgcgcctctc gaacctcccc aaggtctact ggcccgacga cggctacacg
    11641 aagggcgacc tgctcgcgta ctactggaac gtcgccgaca cgatccttcc gtatctgcgg
    11701 gggaggccgc tcacgatgcg gcggatgccc gacgggatcg agggcggcga gttctacgag
    11761 aagaacgccc cctcccacac ccccgaatgg atgccgcgct gccccgtgct cgacgaggag
    11821 ggctcgaaga cgatcgagta tctccgggtc gaggacgagg ccggcctgct gttcgtggcc
    11881 aacctggggg cgatcgagtt ccacccgctc cactccaggt gcgacagcgt cgaccggccc
    11941 gactacctct tcttcgacct cgatccgttc gagccgatca cgttcgacga cgtccgggcg
    12001 gtggcccggc acgtcggcgc ggccctcgac gcgctcgggc tcccctcgta cccgaagacc
    12061 tcgggcgcca ccgggatgca gatctacgtg ccgatcgagc ccgggccgag ctaccagcag
    12121 gtcagggact tcgtcggcgc ggtggggagg ctgatcctga aggccgacgc cgaccgcgtg
    12181 acgatggagt gggaggtctc gaagcgcgcg ggcaaggtct tcatcgacca caacatgaac
    12241 cggcaggggg cgaacatcgc ggcggcctac tcggtgcggc ccgagccggg ggccacggtg
    12301 tcggccccgg tgacgtggca ggaggtcgag gagggaatcg agcccggcga cttcacgatc
    12361 atcacgatgc acgagcggct ggccgaggtg ggcgacctgt tccgcggcgt cctggaggag
    12421 cccgtggacc tgcggccggc gctcgaggcc ctgggcatgg acgcgaacgt tgaggaagcg
    12481 gaggctccga ccgctgccga gagtgagccc gtgtcggagg agctcaccgc cgatccgaaa
    12541 gccgacgacg gggacgccgc ggcccgcctc gccaagtacg gcgagatgcg cgacttcgcg
    12601 gagacgcccg agcccccgcc cgtccccgcc ggcggcgacg ggcgctccgt cgtcatccag
    12661 aagcacgaag ccacccggct ccactacgac ctgcggctgg aggagggcgg ggtgctgatc
    12721 tcgtgggccg tcccgcgcgg gcttcccctg acgcccggcg accgtcgcct cgccgtccac
    12781 accgaggacc accccctcga ctacggagga ttc
//
LOCUS       DASUTW010000125         2213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_298408, whole genome shotgun sequence.
ACCESSION   DASUTW010000125 DASUTW010000000
VERSION     DASUTW010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298408"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VF058_04165"
     CDS             <1..880
                     /locus_tag="VF058_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX6207535.1"
                     /translation="LDVDRFSQGLSFFFNAHIDFFEEIAKFRAARRIWARWMKERYGA
                     EDPQSWRLRFHTQTAGCSLTAQQPENNIVRTAIEALAGVLGGTQSLHTNALDEVLALP
                     TEKAAQIALRTQQVIAHETGVANVIDPLGGSYFVEALTNRSEEQAEEYFRKIEEMGSG
                     SILEGMLVGIERGYFQSEIADAAFREQERYEKGRLIKVGVNEFVDEGDEVIDTLRIGP
                     EVEGEQIASVQRLRGERDDEEAHRALERLKAAAIDEGENLMPPLIDCARAYCTEGEIV
                     GALREIFGSYTETPRF"
     gene            983..1504
                     /locus_tag="VF058_04170"
     CDS             983..1504
                     /locus_tag="VF058_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6207536.1"
                     /translation="MPVADEQASEVASEASADAFEGLEDHYASLRRFAYLITANAAIA
                     EDLAHEAVERSVRAGLDLTGPTAPSYLRTAVVNLWRRTLRRRALEARSLMRVGRVSSV
                     VGDDGVDDRDRVWRALRRVRYEARVCLVLRYYEDLPLAQIAEELGLPLDTVKSRINRG
                     LKDLASHLGERHA"
     gene            1497..>2213
                     /locus_tag="VF058_04175"
     CDS             1497..>2213
                     /locus_tag="VF058_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207537.1"
                     /translation="MHDLEDRLRAALTKHAADVDTSEGEGQVRSQEGGPRMRRAVALL
                     VALGLAGGLLAAGLWVAGRGDQEPAPAEKPEREVIREDPGALVCEEAPRNPQIAANEA
                     WPRTLAGYWIDESEGIVHVAFTEEAEEKVARLTECFPERTFTPVTFEHSLAELEGVLD
                     RMRRDRELIRAGQLTLPGVRDERYSFGLLVMDNVIHVVLEGVTPETIATFEARYGDVV
                     TVEEGYPGGPGGPDPAVNTEA"
BASE COUNT          375 a          674 c          853 g          311 t
ORIGIN      
        1 cctcgacgtg gatcggttct cgcaggggct gtcgttcttc ttcaacgcgc acatcgactt
       61 cttcgaggag atcgccaagt tccgggcggc gcggcgcatc tgggcccggt ggatgaagga
      121 gcgctacggg gcggaggacc cccagtcgtg gcggctgcgc ttccacacgc agacggcggg
      181 gtgctcgctc acggctcagc agcccgagaa caacatcgtc cggacggcga tcgaggcgct
      241 ggccggcgtg ctcggtggga cgcagtcgct ccacacgaac gcgctggacg aggtcctggc
      301 gctgccgacc gagaaggcgg cgcagatcgc gctccgcacc cagcaggtga tcgcccacga
      361 gacgggcgtg gccaacgtga tcgaccctct gggcggctcg tactttgtcg aggcgctcac
      421 gaaccggagc gaggagcagg ccgaggagta cttccgaaag atcgaagaga tgggctcggg
      481 ctcgatcctc gaggggatgc tcgtggggat cgagcggggc tacttccagt ccgagatcgc
      541 cgacgcggcg ttccgggagc aggagcgcta cgagaagggc cgcctgatca aggtcggggt
      601 gaacgagttc gtggacgagg gcgacgaggt gatcgacacg ctgcggatcg gacccgaagt
      661 ggagggggag cagatcgctt cggtgcagag gctgcgaggc gagcgcgacg acgaggaggc
      721 acaccgtgcc ctggaacgcc tgaaggccgc cgcgatcgac gagggcgaga acctgatgcc
      781 gccgctgatc gactgcgccc gcgcctactg caccgagggc gagatcgtgg gcgccctccg
      841 cgagatcttc ggctcctaca ccgagacgcc gcggttctga cgcgcgcgcg cgagccggcc
      901 cggtgggaac cttcgccctg gctcgtgact catctagggt aggaacccga gaaggggacg
      961 actgcgccga ggggatcgag cggtgccagt agcggacgaa caggcctcgg aggtcgcttc
     1021 agaggcgagt gccgacgcgt tcgagggtct tgaggaccac tacgcctccc tccggcggtt
     1081 cgcgtacctg atcacggcga acgcggccat cgccgaggat ctggcgcacg aggccgtgga
     1141 gcgatccgtc cgagcggggc tcgatctcac ggggcccaca gcaccctcgt accttcggac
     1201 ggccgtcgtg aacctgtggc ggcgcaccct ccgcaggcga gcgctggagg cgcggtcctt
     1261 gatgcgggtc ggtcgggtgt cgtccgtggt cggcgatgac ggagtggacg accgggatcg
     1321 ggtctggcgg gcgctgcgaa gggtgcggta cgaggcccgc gtctgcctgg tgctccggta
     1381 ctacgaggat ctgccgctcg cgcagatcgc cgaggagctc gggctcccgc tcgacacggt
     1441 gaagagcagg atcaacaggg ggctgaaaga cctcgcgagt cacctgggag agcgccatgc
     1501 atgacctcga agatcggctg cgggcggcgc tgaccaagca tgcggccgac gtcgacacgt
     1561 cggagggaga gggccaggtt cggtcccaag aggggggacc gaggatgcgg cgggccgtcg
     1621 cgctgctggt ggccctcggc cttgcgggag gtcttctggc cgcggggctg tgggttgccg
     1681 ggcgtgggga ccaggagccc gcgcctgcgg agaagccgga gagagaggtc atccgcgagg
     1741 atccgggggc gctcgtctgc gaggaggcgc cgcgcaaccc tcagatcgcg gcgaacgagg
     1801 catggccgcg caccctcgca ggctactgga tcgatgagag cgaggggatc gtccacgtgg
     1861 cgttcacgga agaagcggag gagaaggtgg ccaggctgac cgaatgcttc cctgagcgca
     1921 cgttcacgcc ggtgaccttc gagcactcgc tggcggagct cgagggcgtc ctcgacagga
     1981 tgcgcaggga ccgagagctg atccgagccg gacagctgac gctgccgggg gttcgcgacg
     2041 agcgctacag cttcgggctg ctggtgatgg acaacgtgat ccacgtggtc ctcgaggggg
     2101 tcacacccga gacgatcgcc acgttcgagg cccgctacgg tgacgtggtc accgtggagg
     2161 aagggtatcc aggaggcccg ggaggccccg acccggccgt gaacacggag gcg
//
LOCUS       DASUTW010000126         2356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_558596, whole genome shotgun sequence.
ACCESSION   DASUTW010000126 DASUTW010000000
VERSION     DASUTW010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558596"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2356)
                     /locus_tag="VF058_04180"
     CDS             complement(<1..>2356)
                     /locus_tag="VF058_04180"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6207538.1"
                     /translation="DREAELAVFDLFLDSIEAGPAAFVVEGEPGIGKSLLWRTCLETA
                     RAARAEVLSARPAESEAKMAFGALDDLLENVLDDALPALAEPKRRALEVALLRREPEE
                     SAPERRLISRAVLDALRLLSTEGSLVVAVDDVQWLDLPSMRVLEFVARRLDEQPIGIM
                     LTRRVEGADERSPPLALDRALPHERLHVLRLDPLGLAAIHELVRSRLDARFPRHVLVR
                     LYEQSGGNPFFALEIARVLITRGLHPGPEEDLPVPDDLRQILAERLAALPPAVREVLL
                     FAAISSEPTIELVEAAHEGAARELLASAAEAGVIELDGEEIRFTHPLLRSVLRGSAED
                     DALTEVNTRLAGVVDIPEERARHLALAATGPDAAVASEVERAARTAAVRGAPTMAAVL
                     LEEAHRLTPQEDEEVRTRRQVEAADYHFRAGDTSRARELLEELTRTIPAGADRAWVLY
                     RLGAVRSQEESWADAERHLRAAMDEAEEDAVLRAQVHQQLIMTRLVAGDLPDAAVHGR
                     ASIALAEEAGDTRTLAYSQAAVASAEFLLGRGIDWKMLKRSEALEQELGEESSTPLPL
                     TRPGVSRGIILKWSDDLPGARPILEESYRHAVETGDDSSIPFILYHLSELECWAGNWA
                     AAARHAEEAHKVSLQSEQEGVRPAALYARALVGAHRGLIEAARADAQESLTLCERSAN
                     VPVMTLALQALGFIDVSLGDPEKAHARLGPMGEMLRVVGLGEPSVVKFLPDEVESLIA
                     LGELDQAKMLVDQFEERAGALDRVWALATGARCRAMLDAATGDME"
BASE COUNT          397 a          831 c          769 g          359 t
ORIGIN      
        1 actccatgtc gccggtcgcc gcatcgagca tcgcccggca ccgggccccg gtagctagag
       61 cccacactcg gtcgagggcc ccggcccgct cctcgaactg gtcgacgagc atcttggctt
      121 ggtcgagctc gccgagcgcg atcagagact ccacctcgtc ggggaggaac ttcacaacgc
      181 tcggctcccc gagtccaacg acacgcaaca tctctcccat ggggcccaaa cgcgcgtggg
      241 ccttctcggg atccccgagc gacacgtcga tgaacccaag ggcctggagc gcgagcgtca
      301 tgacgggcac gttggcgctg cgctcgcaga gcgtgaggga ctcctgagcg tccgcccgcg
      361 cagcttcgat gagtcctctg tgggccccca cgagggccct cgcatagagc gccgcaggac
      421 ggacaccttc ctgctcgctc tgaagcgaca ccttatgagc ttcctcagca tgccgcgccg
      481 ccgccgccca gttcccggcc cagcactcca gctcgctcag gtggtacagg atgaacggga
      541 tcgagctatc gtccccggtc tcgacagcat gacgatagct ttcctccagg atgggacggg
      601 ccccggggag atcgtcgctc cacttgagga tgatccctcg agagactccc ggccgagtca
      661 gtggcagagg cgtcgaggac tcctcgccga gttcctgctc cagcgcctcg gatcgcttga
      721 gcatcttcca gtcgatccct cggccgagga ggaactcagc gctggccacg gcagcctgtg
      781 agtacgcaag cgttcgcgtg tctcctgcct cctcagcgag agcgatcgac gcacgaccat
      841 gcaccgccgc gtccgggagg tcgcccgcca ccaagcgggt catgatcaac tgctgatgca
      901 cctgagctcg aaggactgca tcttcctccg cctcatccat cgcggcgcgt aagtgtcgct
      961 ccgcgtcagc ccagctctct tcctgagagc ggacggcgcc caggcggtac agaacccagg
     1021 cccgatctgc acctgctggg atggttcgcg tcaactcctc cagcaactcg cgggcccgcg
     1081 aggtgtcccc agctcggaag tggtagtcgg ccgcctccac ctgtcgccgc gtgcggactt
     1141 cctcatcctc ctgaggcgta aggcggtgag cctcctccag gaggaccgcc gccatcgtgg
     1201 gggctcctcg caccgcagcg gtgcgcgcgg cgcgctccac ctccgaggcc acggcggcgt
     1261 cgggcccagt ggcggcgaga gccaggtggc gagcacgctc ctcgggaatg tccaccactc
     1321 cggccagtcg tgtgttcacc tcggtcagcg cgtcgtcttc cgcggaaccc ctgaggacgg
     1381 atctcagaag ggggtgggtg aaccggatct cctcgccgtc cagctcgatg actcccgcct
     1441 ccgccgccga cgcaagcagc tctcgcgcgg ccccctcgtg agctgcctcc acgagctcga
     1501 tcgtgggctc ggacgagata gcggcgaaga gcagaacctc ccgcactgca ggcggcaggg
     1561 cggccagccg ctcggctagg atctgccgaa gatcgtcggg gacgggcagg tcctcctcag
     1621 ggccgggatg aaggccgcgc gtgatcaaga ctcgagcgat ctcgagtgcg aagaacgggt
     1681 tgccccccga ctgctcgtac aggcgaacga ggacatgtct ggggaaccgt gcgtcgaggc
     1741 gcgacctgac gagctcatgg atggctgcca ggccgagggg atcgagtcgc aacacgtgca
     1801 ggcgctcgtg gggcagcgct cgatccaggg ccagcggtgg ggaccgctcg tccgctcctt
     1861 cgacacgacg cgtcagcatg ataccgatcg gctgttcatc caatcgccgt gcgacgaact
     1921 ccaggacgcg catcgagggc agatcgagcc actgaacgtc gtcgaccgcg acgacgagcg
     1981 agccctccgt cgacaggaga cggagagcat cgaggacggc ccgcgagatc aaacgccgct
     2041 cgggggccga ttcctccggc tccctcctga ggagcgcgac ctccagggct cggcgcttcg
     2101 gctcagcgag cgccgggagc gcatcgtcga gcacgttctc gaggagatcg tccagggcac
     2161 cgaacgccat cttggcttcg gactcggccg gacgggcgga gaggacctcg gcgcgggcag
     2221 ctcgagcggt ctccaggcag gtccgccaga ggagagactt gccgatgccg ggctcccctt
     2281 cgaccacgaa cgccgcgggc ccggcctcga tgctgtcaag aaacaggtcg aagaccgcga
     2341 gctccgcttc acggtc
//
LOCUS       DASUTW010000127        25023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1075836, whole genome shotgun sequence.
ACCESSION   DASUTW010000127 DASUTW010000000
VERSION     DASUTW010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 25023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25023
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075836"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..961)
                     /locus_tag="VF058_04185"
     CDS             complement(137..961)
                     /locus_tag="VF058_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6207539.1"
                     /translation="MSTSVARTDTSPAAPRRGPWWRRVSPRWIRLASIVVVLIAWEVV
                     GRLNPLFTSYPTAIARAAVDLVQDGRLQPALLTTIQGLGAGYFLAIGIGVPLGFAMAR
                     IRTLNIALDPYVAALYATPRITLIPLLVLMVGIGFQLRLVIVVLSAVFPVIINVRDGA
                     LEVDRNYIDVATSFAASRWQVLRTVVLPGSVPYLFVALRIGAQRAIIGVIVAEMTSAL
                     AGTGILLLDLAQFFRTDAVMVIILIIGFFSILVTAGLRWIQLRVSPWLRSTSEGER"
     gene            complement(965..1762)
                     /locus_tag="VF058_04190"
     CDS             complement(965..1762)
                     /locus_tag="VF058_04190"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6207540.1"
                     /translation="MLKVEGLSHRFPGSEDLVLDDVSLEVPNGGFVSLIGPSGCGKTT
                     LLRIVAGLLEPGGAGQVYVEGKLLLKPSPEKAMIFQHFNLLPWRTALANAAYGLELQR
                     VPKAERRERALAKLKMVGLEAFVDHYPWQLSGGMQQRVGIARALAVEPKVLLMDEPFG
                     SLDALTREYLQTELQKLCEETRVTVLFVTHSIDEAIYLSDRVYVMGTHPGRILEEIVV
                     PLRRPRWTYNARGQREFGQLRDVLWGYLHHELAAHVEAGSEPIGYQE"
     gene            complement(1894..2985)
                     /locus_tag="VF058_04195"
     CDS             complement(1894..2985)
                     /locus_tag="VF058_04195"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6207541.1"
                     /translation="MAFEPKGKRWLRVFAAVFGLLLLAAACGDNGGDNGGNGDNGGGN
                     GEGTEEPGGGGGDPSAPEQTTLRIVVAPPEFDSLVDAIWFQKLEEAGITVETVDFEFG
                     PDTTRALASGAGAIASTSPTPLMRYIQEAGGGMKIIAAALLNTDYVLLTTPDIESAAD
                     LVGKRFGISEPGDISDSTTRLLLDGHPDLDISEVEIVQIGGTSDRIAALVAGEISGGP
                     VHAADGLAAAEEAGLVPLINYWEYIPIYAQRFIVADEQWLGENPNLAQLAVDSLIEAQ
                     REAQNDKEAFISLSQELIPDMPDEIRDDVYDFFADGGFFAVNGGIDAIMPTIEADLSI
                     GNLEGELPDQSVWLDTSFVENYLEREGEV"
     gene            3239..4414
                     /locus_tag="VF058_04200"
     CDS             3239..4414
                     /locus_tag="VF058_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6207542.1"
                     /translation="MLEELVSIESHSFRRDGVHRVGEVVVGLLKEAGVRIERVPRPPL
                     DPELRWLAEVLSPEVPDEELADSFVGRRPGSDSGRVLLLGDLDTAFPPGALEDFPFAT
                     RGDRAYGPGVADMKGGLVVLVAAVHALHALDLEAPPLSVVLCGDEQAGSMGSRSLVER
                     EAGQSRWCLCVECARDGGRLMSSRAHIGVGLVEAKGRGAHAGTARSAGANAIDALVAA
                     LTAASLVDDPAGASLVTATIVGGGTRRSMVPELARAVLDLRCPDAAAWEALTMSLDLA
                     VRAAVVPTGTEVRLRTAAHRPGFGPEQGAELLATVRRVGLDIGLDLQAVPSHAAGSSS
                     FAAASGVAVVDGMGPSGGGLMTPEESIDLRSLTQRAAVLAGVMHHLARTDGAEAEDG"
     gene            4411..5919
                     /gene="argH"
                     /locus_tag="VF058_04205"
     CDS             4411..5919
                     /gene="argH"
                     /locus_tag="VF058_04205"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX6207543.1"
                     /translation="MSDDATMQHGRLGGPPSAVNLEHSGAPHLRYEVRMLRAFAAVDM
                     AHTVMLAEQGILTEAQAGAILSALREIDELGPEGFPTDPRFGSILLQVERYLAERIGE
                     DTAGRMHTGRSRNDQSTAASRLHSRDLLLEVLRMLQRLQGLILDLAEQHVDTYIPGYT
                     HLQHAQPSTLAHYLLRHWFTLERDQQRIEGAFLRTNLSALGGAAMTGTSWPLDRQRAS
                     DLLGHERPVENAYDAGIFTRDYPSEIAAVLSILVNDVGRIAGDLYLWSTWEFAFVEID
                     DSLAGTSSIMPQKKNPHALERVRGLAGRSIGWLPATLGMLRSVSSSDLDLLFGEELTN
                     EAARDTISVLALTHASLETIVFKTDVMRERAGVYWSTAANLADEVVRATGLPFRSVHG
                     MVGRLVRLAIERSLAPEDVNAALLDEAAEQVLGRPLGVPDDLVRGALDPVGAVQGLVT
                     TGSAHPDAALAQIATARGYEVEHQAWLEAKDRQLSDAAAELALAADRLIGSA"
     gene            complement(5940..7340)
                     /locus_tag="VF058_04210"
     CDS             complement(5940..7340)
                     /locus_tag="VF058_04210"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HEX6207544.1"
                     /translation="MGGTGLVRIGGGTIQTPDGSLPADLLIADGRVQAVLDRDADVAG
                     AETVDADGLWVLPGLVDLHAHTRVPGYEYKEDYATASRAAAAGGVTTFVDMPNVEPPT
                     TTLELFEEKRAVAARDSVIDYGHFVGPTDLEQIPLFAEAGVTGFKIFQVTGGYPHDPR
                     LAIDDPGRLYATFKAVARTGLTCVVHPFAQTLFETLSEEAFAEGAPRDITTFSRIYTM
                     DVVWRTAVAILIELQRETGVHLHVVHTHAPGSLRLLREAKRQGMSVTAACDPKYTHLT
                     DKELEEQGARGIPGGFVTGDEDRMREVWRSIDDGTIDVMDSDHAPHTLEDLERMEADP
                     WTGPFGAPHYDHILALLLTDVREGRMRLERLVDMVCATPARIIGQYPEKGALLPGSSA
                     DVVLVDPEAEVRPEDGKMYSKSGWSPYVGWVLWGRPVLTMLRGEIIARDGEVVTEPGS
                     ARYVPGVPRPPGPLPR"
     gene            7440..8213
                     /locus_tag="VF058_04215"
     CDS             7440..8213
                     /locus_tag="VF058_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX6207545.1"
                     /translation="MDPDQIAFILAAMGASFASGVLSTVSGLGGGVAIIALLAIFVPP
                     HLVVVFSSPVLLVGNTSRFLLFRRHVDRKLLGWFLVGGIPFAVAGSMLLPHIPVRTLE
                     LALAGFMLIFVLESLLTRNWREEARSRPVRQGMAWLVRPATSFAVVGAGAGFLSSTVG
                     ASGPFIAPFLATKGLSKQRFVGTISGGTTALNVAKISTFASVGLLGVENVPALVGAAV
                     AIVGGNLVGRRMLDRASHRTFAILLITVIAVSGVQLLIG"
     gene            8336..9406
                     /locus_tag="VF058_04220"
     CDS             8336..9406
                     /locus_tag="VF058_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX6207546.1"
                     /translation="MVEAGVEHLVVAGSNRFGPAVEWLSGWPTTTESMLLVGPEEPDV
                     LLIQLRNHVPNARSLAAACDVRWGGHVTVDAAVGELRRRGGGRVGVMGPVSHRAHRTL
                     EEEFDEPVDLDGAYVRMRLVKSEEEMAWVRYGVELTDRAIDALLAHGGPGLTVEELVD
                     VVERAYVPLGGGTHIHFFAVTPMDNPSQCVPAQWPSRRRLRPGDAVTTELSASYRGYP
                     GQALRTLSVAAEPNALHRRLYAVAEEAFEAIIAAIRPGAHPRDLVEASSPIEDAGMTI
                     YDDLVHGFVGGYLPPVLGSRSRTLGAIPDLPLEEGMTVVVQPNVITPDERAGVQIGEL
                     VRVGPAGAERLHRAPRGFLRMG"
     gene            9406..10632
                     /locus_tag="VF058_04225"
     CDS             9406..10632
                     /locus_tag="VF058_04225"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6207547.1"
                     /translation="MGGRGEPSPQSEVSIGRVLFQRNFFPYFLGNLISNSGTWFQSVA
                     QVLFVYRLTGSLFMVGVVNFAQFAGMLIVTPVGGSYADRFDRRKLLIRVNLATAALAG
                     LLALLTAMDLITLPILFTLVLLIGTAVALITPTMQALISALVPRHQLPKAIAMNGVTF
                     QTARVLGPILGALVVDRFGFAWAFSVNALSFVALAASVWAVHPITPQARAKGRPPMWE
                     GLRVAREDRALAAVLSVGIAVSFAMDPLITTGAEFATEEFGRADTLVGLLIAVYGAGA
                     IVAAFTRSWEESTSPRRVGASVSMIAAGMVAFSLAPTLAVALPVLFIAGYGHLSSTAA
                     SLATVQLAVPDEMRGRIMALWTITFLGMRPLASLLDGTLASVAGVRWAAAAMSVPAVL
                     VAMLLFSTNRGTREDG"
     gene            10733..12097
                     /locus_tag="VF058_04230"
     CDS             10733..12097
                     /locus_tag="VF058_04230"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HEX6207548.1"
                     /translation="MKVDLVIRGGSVWQEGQGFINADVAVDGERIVAIGAPDAGFPEG
                     RRVVDAGGLTVIPGLIDTHIHLRDPGFTYKEDYETGTRASAKGGVTMVVDMPNTNPVP
                     NTIERFMEHREIASGKSLVDFNHWAAPTILDQIAPIVAEGAAGFKVFMISHHYPYDNP
                     DQFIPLDDPLRWHDTMTEIARTRRPLIVHPHNQNMWMTLADKYEVEGRTTPEDREEAY
                     IFADNFIQTSSVASLLLLARSTGVDLRILHNNWMPLLEMVRMFKASGYRAIFEQNPWA
                     VFGFGVKDQVKDADEVWRTLNDGTIDIIASDHAPHSPEEVATAQINAFRSVIVSITLE
                     EHMLAMYLTEINDQGRITLDRLVHLFSTNVAKHLGVYPKKGTLRVGSDADITLIDMEK
                     EDVIDQARLESKCGITPYEGWKLKGLPVGTIVRGQFVMEDGTVTGDPGYGRFTAPLGP
                     GTLR"
     gene            12100..13074
                     /locus_tag="VF058_04235"
     CDS             12100..13074
                     /locus_tag="VF058_04235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6207549.1"
                     /translation="MGRYGVLLPHFGPHASRDTVAREAVRLEELGFDSVWARDHVVYE
                     PHEYEHPDTTFLDPIVVLSGVAAVTSHIGIGTGTLIPHRHPVAAALALSSLDALAGPG
                     RLTVGIGLGSFGHEFDAVGMKGWDRREVVEEQVGIFRRLWTGEPVTHHGRFYSFEEVA
                     ISPVPKARDLPIWYSGTSKAAVRRAVEYGDGWLPGSLPRAEYGDRRQRMEALAEAAGR
                     AVPDSGLIPFVSPGRTVEEAVAAVPMEAILAMANKRYRRPDGFATLEDLDGTLMAGPP
                     ERLSEEIRRYEELGAGTFVLDLRFRFDDWRECTELLASELLPQVRSGD"
     gene            complement(13071..13553)
                     /locus_tag="VF058_04240"
     CDS             complement(13071..13553)
                     /locus_tag="VF058_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018505815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6207550.1"
                     /translation="MRLTVNGSLAEASVEPRDTLADLLRERLGLTGTKVGCEHGVCGA
                     CTVLLDGSSVRSCLVLALQADGRVVDTVEGLAGDEQLHPIQRAFNVAHAVQCGFCTPG
                     MLMVVAELLAEEAAPDERRIRERVGGNLCRCTGYENIVRAVQLAVGDTPTRGGDAGRA
                     "
     gene            complement(13565..14449)
                     /locus_tag="VF058_04245"
     CDS             complement(13565..14449)
                     /locus_tag="VF058_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX6207551.1"
                     /translation="MKLPAFSYVAPTSLGEAVEALAAGAPDAQPLAGGQSLLPAMALG
                     MVRPTMLVDLGLVPGLDALEAAGHGTVVIGAMARQRAVERHPELLRRCPAIGEAAGHV
                     GSVPVRNRGTVGGSIANADPGAEWPCLLLALDGEVDATGPEGSRTIRGVDLYPAPFAA
                     ALAPGELLTEVRLRLPAGPAGAAFRELALRSGASAIVGVAAVLGTDPVGTVHHARVAV
                     LNGGSTPMRADAAERVMLGRAPTDDAVDEAIAAVRDEVDPPGDRSFSAGYRRHLAGTL
                     AGRALRAARDRIGGRAAW"
     gene            complement(14499..15014)
                     /locus_tag="VF058_04250"
     CDS             complement(14499..15014)
                     /locus_tag="VF058_04250"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6207552.1"
                     /translation="MSTNPEALRLQVGGKVEYDWGTLWPAYHKEKMNWATGFRTRGDV
                     DILFLSGTTGRDPFNDAPARTPEEERAGRGNVVQGGMRAQTRQCLQEIKDNLEIMGAS
                     LKSIVMFRYYVKNREQVFDMRDEMYAFFEEHEPDLREHPRPATLLRGVGIDLPDMLIE
                     IEAWAVVPRSG"
     gene            15051..17204
                     /locus_tag="VF058_04255"
     CDS             15051..17204
                     /locus_tag="VF058_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005150523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX6207553.1"
                     /translation="MLSCATRPEFRRPAGKETHISPRATTRPGFRIAVDIGGTFTDLV
                     AVGTGRVVAAAKVLTTPADPSEAVERGVGEILDGIDPAGVGEVVHGTTLVSNSLIERK
                     GARTALLTTKGFRDVLTIRRELRYDLYDLFLEMPEPLVPRRLRWEVDERILADGSVDR
                     PVDPEDVRRIARRLEREGVQAAAISLLHSYRDPCHEQTVAAALREVLPDLPVSLSSEV
                     APELGEFHRTSTVVANAYVMPLVARYLERLRERLAAMGLRSPLHIMLSTGGGGLAGVD
                     TAKRFPVRLLESGPAAGALSAGFWGIRTGHRDVLGFDMGGTTAKAVLIEDGLPLVTRE
                     HEAARVYRFTKGSGLPLKVPVIDLIEIGAGGGSIARVGPFGLPKVGPESASSDPGPVC
                     YGLGGKEPTVTDADLVLGFLNPAFFLGGDMALDLDAARDSLSRLGDELGLPVEGTAAA
                     VHRVVNENMASAARMHAIERGRDIRRFAIVATGGAGPVHAWGVARGIGIRTLVFPPSA
                     GVASAFGMLTANPGFEFARSLPCALGDVRWGEVRAAIATMAREGTRQLRQAGVRRDDV
                     AITLAVDVRHRGQGDPVTVELGDALHRKDPAVQVEEAFEATYVRSYGRRPPGVEPEIM
                     TWRVRVMGPEPQLDVRARRRADTARPALKGRRRVWFLESGRFVSANVYDRYALGPGDV
                     VKGPAVVEERESTAVIGPGSTGRVDEVGTLVVTVR"
     gene            17249..18931
                     /locus_tag="VF058_04260"
     CDS             17249..18931
                     /locus_tag="VF058_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX6207554.1"
                     /translation="MDPVTLEILWTRLVTVTNEQAAALQRTSFTPIVRESGDLSAAVF
                     DRRGRMLAQAVTGTPGHINSLATSMTHFLAAFPPETLQPGDVLITNDPWMTSGQLNDL
                     SVVTPVFHRGRLVGFFGNTCHAMDIGGRGLSADAGEVFEEGLSIPVTKLFDAGRPNEE
                     LLRILQANVRAPFEVLGDVHAQVAGNQVGAERLLEYLEDFGLDDLEDLAEEILGRSEQ
                     AMRDAIRTLPDGDYRHTIQTDGLDEPVTIACRVSVQGDRIHVDYSGSSPQIDRGMNVV
                     LNYTAAYTSYALKCAISPEVPHNDGSFRPVSTSAPEGSILNALFPAAVAARHIVGHFL
                     PHAVFGALVQIVPDRVLAEGAGNIWLTTVRGAGADRFITVFFAAGGMGARPTADGLST
                     TSFPSGIATAPVEVIESTSPLVFRRKELRRDSGGPGRFRGGLGQRIEVEVRTGEPFIV
                     SSLADRMRIRAEGYLGGLPGGAGGFSTSLGTKPNPKLSLPLQAGTRFTLDLPGGGGFH
                     PPVQRNPAAVARDVAEGLVSPTSAKRDYGVSVTPSGRVKGTVSRRGRGRRGR"
     gene            complement(18897..19913)
                     /locus_tag="VF058_04265"
     CDS             complement(18897..19913)
                     /locus_tag="VF058_04265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6207555.1"
                     /translation="MERTGFAAGYDPAMGLRDMARWMRAAEEQGFEIGFFSETIELMR
                     DSVSALATIGLATDRLVIGCTQIVRLRSPLVMAQTLASLDEVTGGRILLAPGACTSTH
                     AKRHGLEHLDPGVTLREWVEAIRAILTGETVSYRGKVVHFDDVRLGWTPPRQDIPIFI
                     PATSRKGLRIAGEIGDGVVLNAVCSPEYSENAIAIVREAATEAGRDPDAIAIYQLVNC
                     SIEDDRVAALDAIRWEVASKLDPIQLPFIMGPKKLVGEPYMHEADVPIFQEAWARGGK
                     EELIRVVPDSYIEGMTASGKPDEVVARVQRYRDVGVQVPILRPAAPHQARRLIDLFAP
                     VRAG"
     gene            19981..20583
                     /locus_tag="VF058_04270"
     CDS             19981..20583
                     /locus_tag="VF058_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX6207556.1"
                     /translation="MGASGAADHVDQILAEWRRERPDLDFSAMAVIARISRLSRILER
                     RIETVLSRYGINESGFGVLAALRRAGPPYRLTPTGLYNSLLISSGAMTNRLDRLTAAD
                     LVERIPDLDDRRSVLVRLTTRGLRVVEEALDAHIENEIALLGALSARDKQELAGILRR
                     LLVGFESDAGPHAVLDEDDAPGRDGLRRRSSLHPPGDAAP"
     gene            complement(20537..21292)
                     /locus_tag="VF058_04275"
     CDS             complement(20537..21292)
                     /locus_tag="VF058_04275"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX6207557.1"
                     /translation="MWSVALSGRTALVTGAGRGIGRAIALELARAGSDIIAVARTGGQ
                     LEEVARDVRALGPEALALPADIAHVDRLPEVVAEAWRWRGRVDVLVNAAGVIDRTQPP
                     DIRPSHWDPVFATNVRGTYFLSQAVGARMFERGEGAVVSIASLAGERVTGASVTYQAS
                     KAALIQITRALAVRFAPAVRVNAVGPGYIRTSLNEEWLDRPENRSYVEERTPLRRVGT
                     PDDVAGAVVFLASPAASYITGQHLRVDGGWSVA"
     gene            complement(21292..21582)
                     /locus_tag="VF058_04280"
     CDS             complement(21292..21582)
                     /locus_tag="VF058_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207558.1"
                     /translation="MMDESDVLVARAMWVAALPAKAELVDAAAAPVWGDGALLRTTTP
                     LSLAEISEVPPAGGADGDGIPEGIQLAGSAVRDDRLLEVARSLQRFIRGGGR"
     gene            complement(21848..22243)
                     /locus_tag="VF058_04285"
     CDS             complement(21848..22243)
                     /locus_tag="VF058_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207559.1"
                     /translation="MGPVEPRAAPAAAQPPAVEAGAVPAEFCVDTGWSIRSLSALCGL
                     AGPHLNPDQVMPSDVVSLASSTDDAGSIAMWVKACRVLFDAFGVRDGSSALRPDRAAE
                     PITRGGRRLRVGRAPFAGLAETAHEAERT"
     gene            22751..23755
                     /locus_tag="VF058_04290"
     CDS             22751..23755
                     /locus_tag="VF058_04290"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6207560.1"
                     /translation="MSAVIDAHSHLFPPGFIERLRARPTAPRITGGPDGDRLIVFASE
                     ERTGGRPVGRSFIDPAEKLRFMDRLGIDRAAVQMANPLLEPFDGQEAVDAAAELNAEM
                     AGLLRASGGRLAGLGALPQHDLAAAAAAAEQVAATDTLFGIASGPLLCRRQLDDPELD
                     PLWGLLERLDVPVTIHPQRGLPVPELEGHGLALSLGLSLPFETTVAVARLVLGGVLQR
                     FPGLRILAAHGGGALPFLADRLDAVWRTDPRAREQMPLPPSTDLGRLWLDGLVYGPAP
                     LRAALALAGPERVLFGTDHPYVVADAERNLAAIDEATDGGADALAVRGGVAERFFGLT
                     "
     gene            23826..24554
                     /locus_tag="VF058_04295"
     CDS             23826..24554
                     /locus_tag="VF058_04295"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HEX6207561.1"
                     /translation="MSTADLPATMRRHPVPGAVVREWVIPAKGYDAFMVSARDVVRFV
                     DVEGKQVPDVICYDPSDPTDELSMGNSLLLNKRRELRRGDVLYSVTCHKMMTIVGYSN
                     EESFAYGPMCSEEVNQLRYRVPGTANCRDNFAMALAAAGMSVDRRRIPDAFVPFMRVE
                     VDEDGTLEIREPTSEPGDHYDLRAEMNLLLAVSNCPQKRNACNGFNPTPMGVIVYRPD
                     AAAPEPPGAGRGIRTDSSAGLPRR"
     gene            complement(24738..>25023)
                     /locus_tag="VF058_04300"
     CDS             complement(24738..>25023)
                     /locus_tag="VF058_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010309280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX6207562.1"
                     /translation="NQSGGVIDACTEAEVVDGIRLLAETEGVFTETAGGVTIAVLKRL
                     AESGAIRPDEETVAYVTGNGYKTIEALEGHVAPSYHVAPDLDEFLTAIGA"
BASE COUNT         3667 a         8749 c         8774 g         3833 t
ORIGIN      
        1 accgcggtga ccaggcggat cgtccagacc ccgatcggtc ggtgggtgaa ggcacgccga
       61 tccaccgcgg actggtccga tgggatgcga cgctcttccg tcctctcgga cggccggacc
      121 cgctcctctg ccctgctcat cgctccccct cgctggtgct ccgcagccac ggactgaccc
      181 ggagctggat ccagcgcagg ccggctgtga cgaggatgct gaagaagccg atgatcagga
      241 tgatcaccat cacggcgtcg gtccggaaga actgggccag gtcgagcaga agaatccctg
      301 tcccggcgag ggcggaggtc atctccgcaa cgatcactcc gatgatagcc cgctgggccc
      361 cgatccgcag cgccacaaac aggtacggca ccgacccggg cagaaccacg gtccgcagca
      421 cctgccagcg agacgcggcg aaggacgtgg ccacgtcgat gtaatttcgg tccacctcga
      481 gcgccccgtc acgcacgttg atgatgaccg ggaacacggc gctcagcacc acgatgacca
      541 gtcggagctg gaacccgatc cccaccatca gcacgaggag cgggatcagc gtgatacggg
      601 gcgtcgcgta cagagcggcg acgtaggggt ccagggcaat gttcagtgtc cggatccgag
      661 ccatggcgaa cccgagcggc accccgatac cgatggccag gaagtacccg gcgccgaggc
      721 cctggatcgt cgtgaggagc gctggttgaa ggcggccgtc ttgcaccaga tcgacggcgg
      781 ccctggcgat cgcggtgggg taggacgtga acagggggtt cagccggcct acgacctccc
      841 aggcgatcag cacgaccacg atcgaggcca gccggatcca ccgtggggac acccgcctcc
      901 accacgggcc gcggcggggc gcggccggtg aggtatcggt gcgagcgacg gacgttgaca
      961 cggcctactc ctgatatccg atcggctcgc tccccgcttc gacgtgcgcg gcgagctcgt
     1021 ggtgcaggta cccccaaagg acgtcccgga gctggccgaa ctcgcgctgc ccacgggcgt
     1081 tgtaggtcca cctaggccgc cggaggggca ctacgatctc ctcgaggatc cgccccgggt
     1141 gcgtccccat cacgtacacg cggtccgaaa ggtagatggc ctcgtcgatc gagtgggtca
     1201 cgaacagcac cgtgacccgg gtctcctcgc agagcttctg taactccgtc tggaggtact
     1261 cacgggtgag tgcgtcgagc gagccgaacg gctcgtccat gaggagcacc ttcggctcca
     1321 ccgcgagagc ccgcgcgatg ccgacccgct gctgcatccc ccccgagagc tgccaggggt
     1381 agtggtcgac gaacgcctcc aggccgacca tcttgagctt cgccagggcc cgctcccggc
     1441 gctctgcctt ggggacgcgc tggagctcca ggccgtaggc ggcgttggcc agcgctgtcc
     1501 gccacggcag gaggttgaag tgctggaaga tcattgcctt ctctggcgag ggcttcagca
     1561 acagcttgcc ctcgacgtac acttggccgg cgccacccgg ctcgaggagg ccggcgacga
     1621 tgcgcagcag cgttgtcttg ccgcaaccgc tcggcccgat cagtgagaca aacccgccgt
     1681 tgggcacctc gaggctcacg tcgtcgagca cgaggtcttc cgacccgggg aaccggtggg
     1741 acagtccctc gaccttcagc atgggatcga tcatgcctcc ttgtggttgc ggggagtcgc
     1801 ccttggtgca gacctcagtc cggcacccaa cgcgaccccc cgctcccggt actcggcgcc
     1861 gcggcgccgt gctacgcggg tcgtttcccg aggttagacc tctccctcgc gttcgaggta
     1921 gttctccacg aacgaggtgt ccagccacac cgactggtcg ggaagctcgc cctcgaggtt
     1981 cccgatggac aggtccgcct cgatcgtcgg catgatcgcg tcgatcccgc cgttcacggc
     2041 gaagaacccg ccatccgcga agaagtcgta gacgtcatcg cggatctcat ctggcatgtc
     2101 ggggatgagc tcctgcgaca gcgagatgaa cgcctccttg tcgttctgtg cctcgcgctg
     2161 cgcttcgatc agactgtcga ccgccagctg ggccaggttc gggttctccc ccagccactg
     2221 ctcgtcggcc acgatgaacc gctgagcgta gattgggatg tactcccagt agttgatcag
     2281 ggggacgagg ccggcctcct cagccgccgc gagtccgtcg gcggcgtgaa ccgggccacc
     2341 cgagatctca cccgcgacga gcgcggcgat ccggtcgctc gttcctccga tctgtacgat
     2401 ctccacttcg gagatgtcga ggtcgggatg accgtcgagc agcaaccggg ttgttgagtc
     2461 gctgatgtcg cccggctccg agatgccgaa cctcttcccc acgaggtcgg ctgcggactc
     2521 gatgtcgggc gtggtgagca agacgtagtc ggtgttcagc agcgcggccg cgatgatctt
     2581 catcccgccg cccgcctcct ggatgtaccg catcaggggc gtcggggacg tgctcgcgat
     2641 cgcgccggct ccggatgcga gcgcccgggt ggtgtcggga ccgaactcga agtccacggt
     2701 ctcgacggtg atcccggcct cctccagctt ctggaaccag atcgcgtcca ccagcgagtc
     2761 gaactcaggt ggcgcgacga cgattctcag cgtggtttgc tccggagcgg acgggtcacc
     2821 accgccgcca ccaggctcct cggttccctc gccgttgccg ccaccgttgt caccattgcc
     2881 accgttgtcg ccgccgttgt ctccgcaggc cgccgccaac aggagcagtc cgaagacggc
     2941 cgcgaagacg cgcaaccagc gcttcccctt cggttcgaac gccatgctcc cctaccccct
     3001 ctcgacgagt gccgtgcccg aaccgccgtc tccctcctcg agtcatcccc tccttccgat
     3061 ttaatatatg gtccgccagg ctagcgacct ccgaaatcgc ggtcaagcgg ggtgggtgcc
     3121 gcgttggagg tcgaccgagt cggaggacat cgccatcagt ccgagcacca cagccgagga
     3181 cgacccgacc gggcgggctt tgcgttggct cgacgcctcg gcaccgaggt ggctcgcctt
     3241 gctcgaggag ctcgtctcga tcgaaagcca ctccttccgc cgcgacgggg tccaccgggt
     3301 gggcgaagtc gtggtcggac tgctcaagga agccggcgtc cgcatcgagc gcgtcccccg
     3361 cccccccctc gaccctgagc tccgatggct cgccgaggtc ctgtcgcccg aggtccccga
     3421 tgaggagcta gcggacagct tcgtggggcg tcgtcccggc tcggactccg gccgggtcct
     3481 gctcctcggc gacctcgaca ctgctttccc tcccggcgcg ctggaggact tcccgttcgc
     3541 gacccgcggc gaccgcgcgt acgggccggg tgtagcggac atgaagggag ggctggtcgt
     3601 cctggtggcc gcggtccacg cgctgcacgc cctggacctg gaggccccgc cgctgtcggt
     3661 ggtgctgtgc ggggacgagc aggccgggag catggggtcg cgatcgctgg tggagcggga
     3721 ggcagggcag agcaggtggt gcctgtgcgt cgagtgcgcc cgtgacggcg gcaggctcat
     3781 gtcgtcccgg gcgcacatcg gtgtcggcct ggtcgaggcg aagggtcgag gcgcccacgc
     3841 cggcaccgcg cgctcggccg gggcaaacgc gatcgacgcc ctcgtcgcgg cgctcacggc
     3901 ggcgtcgctc gtcgacgacc cggccggggc cagcctggtc actgcgacga tcgtcggcgg
     3961 cggcaccagg cggagcatgg tgccggagct ggcccgggcg gtgctcgacc tgcggtgccc
     4021 cgacgccgcg gcgtgggagg ccttgacgat gtccctggac ctggcggtcc gggcagcggt
     4081 tgtcccaacg ggcaccgagg tgcggctgcg gacggcggcc caccggccgg ggttcggccc
     4141 ggagcagggc gccgagctgc tcgccacggt gcgacgggtg gggctcgaca tcggcctgga
     4201 cctccaggcg gtgccgtcgc acgctgcggg gagctcctcg ttcgcggcgg cctcgggggt
     4261 cgccgtggtg gacggaatgg ggccgagcgg cggtgggctg atgaccccag aggagtcgat
     4321 cgacctgcgc agcctgaccc agcgggcggc ggtgctggcc ggcgtgatgc accacctggc
     4381 acggacggac ggagcggagg cggaggacgg atgagcgacg acgccacgat gcagcacgga
     4441 cgcctgggag ggccgccgag cgcggtgaac ttggagcaca gtggcgcgcc gcacctacgc
     4501 tacgaggttc gcatgctgcg ggccttcgcg gccgtggaca tggcccacac ggtgatgctc
     4561 gccgagcaag ggatcctcac ggaggcccag gccggggcga tcctcagcgc cctccgggag
     4621 atcgacgagc tggggccgga ggggttcccc accgaccccc gattcggctc gatcctgctc
     4681 caagtggagc gctacctagc cgagcggatc ggcgaagaca ccgctgggcg gatgcacacc
     4741 ggacgcagcc ggaacgacca gagcacagcg gcgagccgct tgcactcgcg ggacctcctt
     4801 ctcgaagtgc tccggatgct gcagcggctc caaggcctga tcctggacct ggccgagcag
     4861 cacgtcgata cgtacatacc gggctacacg cacctccagc atgcgcagcc gagcactctc
     4921 gcccactacc tcctccgcca ctggttcacg ctggagcggg accagcagcg catcgagggc
     4981 gccttcctgc ggacaaacct cagcgccctt ggaggcgcgg cgatgaccgg cacctcgtgg
     5041 ccgctcgacc ggcaaagggc gtccgacctc ctcggccacg agcggccggt tgagaacgcg
     5101 tacgacgcgg ggatcttcac ccgcgactac ccgtccgaga tcgccgccgt actgtcgatc
     5161 ctggtaaacg acgtggggcg gatcgccggc gacctatacc tgtggtccac ttgggagttc
     5221 gcgttcgtcg agatcgacga ctccttagcc gggacgagca gcatcatgcc ccagaagaag
     5281 aatccccatg ccctggagag ggtgcggggc ctggccggcc gctcgatcgg gtggttgccg
     5341 gcgacgcttg gcatgttgcg ctcggtgtcg tcatccgacc tcgacctgct gttcggggag
     5401 gagctcacca acgaggccgc ccgggacacg atcagcgtgc tggcgctcac ccacgcgtca
     5461 ctggagacga tcgtcttcaa gaccgacgtg atgcgggagc gagccggcgt gtactggagc
     5521 accgccgcga acctcgccga cgaggtggtg cgggccaccg ggctcccctt ccggtccgtg
     5581 cacggcatgg tcggccggct agtccggctg gccatcgagc gaagcctcgc tcccgaggac
     5641 gtgaacgcgg ccctgctcga cgaggcggct gagcaggtcc tgggtcggcc gctcggggtc
     5701 cccgacgacc tggtacgcgg ggcgctcgac cctgtgggcg cggtgcaggg gctggtgacc
     5761 accgggtccg cccaccccga cgccgccctg gcccagatcg cgaccgcgcg cggctacgag
     5821 gtggagcacc aggcctggtt ggaggcgaag gaccggcagc tgtcggacgc ggccgccgag
     5881 ctcgccctgg ccgcggaccg gctgatcggc tccgcctgag ccggcccgcg gtggggacgt
     5941 cagcgcggga ggggccccgg cgggcggggc acgccaggca cgtaccgagc gctgcctggc
     6001 tccgtcacga cctcgccgtc gcgggcgatg atctccccac ggagcatcgt gagcaccggc
     6061 cggccccaga ggacccaacc cacgtaggga ctccaacccg acttggagta catcttgcca
     6121 tcctccgggc gcacctcggc ctcggggtcc accagcacga cgtccgcgga ggaccccggc
     6181 aggagcgccc ccttctccgg gtactggccg atgattcggg caggggtggc gcagaccatg
     6241 tccaccagcc gctccaaccg cattcgcccc tcccgcacgt cggtgaggag cagcgccagg
     6301 atgtggtcgt agtgcggcgc gccgaacgga ccggtccacg ggtcggcctc catccgctcc
     6361 aagtcctcga gggtgtgcgg ggcgtggtcg gagtccatga catctatcgt gccgtcgtcg
     6421 atcgacctcc agacctcccg cattcggtcc tcgtcccccg tgacgaaccc gccagggata
     6481 ccccgggccc cctgctcctc gagctctttg tcggtgagat gggtgtactt cgggtcgcag
     6541 gcggcggtga cgctcatccc ttggcgcttg gcctctcgga gcagacggag cgacccgggc
     6601 gcgtgagtgt gcacgacgtg gaggtgcacg cccgtctccc gttggagctc gatcaagatc
     6661 gccacggcgg tccgccaaac gacgtccatc gtgtagatcc ggctgaaggt cgtaatgtcg
     6721 cgcggggcgc cctctgcgaa cgcctcctcg ctcagcgtct cgaagagggt ctgggcgaag
     6781 ggatggacca cgcaggtcag gccggtccgg gcgaccgcct tgaaggtcgc gtacagccgg
     6841 ccggggtcgt cgatcgccag ccgcgggtcg tgcgggtagc ccccggtgac ctggaagatc
     6901 ttgaacccgg tcacccccgc ctcggcgaat agtgggatct gctccaggtc cgtcggcccg
     6961 acgaagtggc cgtagtcgat cacggagtcg cgcgcggcga cggcgcgctt ctcctcgaac
     7021 agctcgaggg tggtcgtcgg gggctccacg ttgggcatgt ccacgaacgt ggtcacccca
     7081 cctgcggccg cggcgcggga cgccgtggcg tagtcctcct tgtactcgta gcccgggacc
     7141 cgcgtgtgcg cgtggaggtc gactaggcca ggcagcaccc acaggccgtc ggcgtcgacg
     7201 gtctccgcgc cggccacgtc ggcgtcacgg tcgagcaccg cctgcacgcg gccgtcggcg
     7261 attagcaggt cggcggggag cgagccatcc ggcgtctgta tggtgccccc cccgatccgc
     7321 accagacccg tcccacccat ccgcgacccc ctttgtccgg aacgatgcga ggcctccccg
     7381 ccactgcgtg accgggatac ccggatcgcg gacgagaggg atgtactata tcgagcctcg
     7441 tggaccccga tcagatcgcg ttcatcttgg ccgcgatggg ggcgtccttc gcgtcgggcg
     7501 tcctcagcac ggtgtccggg ctcggagggg gcgttgccat cattgcgttg ctggccatct
     7561 ttgtgccgcc gcacctggtg gtggtcttct cctcgcccgt gctgctggtg gggaacacct
     7621 cccggttcct gctgtttcgc cggcacgtgg accgcaagct cctcgggtgg ttcctcgtgg
     7681 gaggaatccc gttcgccgtc gccggctcga tgctcctccc ccacatcccg gtccgcacct
     7741 tagagctcgc cctggccggc ttcatgctga tcttcgtgct ggagagcctg ctcacccgga
     7801 actggcggga ggaggcgcgg tccaggcccg tccgccaggg gatggcctgg ctggtccgac
     7861 cggcgacctc gttcgccgtg gtgggggccg gggcggggtt cctgtcctca acggtcgggg
     7921 cgtccggtcc attcatagca ccgttcctgg ccaccaaggg cctgtccaag cagcggttcg
     7981 tgggcacgat cagcgggggg accaccgcgc tgaacgtcgc caagatctcc acgttcgcct
     8041 ccgtggggct cctcggcgtg gagaacgtcc ccgcgctggt cggcgcggcc gtggccattg
     8101 tgggggggaa cctggtcggc cggagaatgc tcgaccgggc cagccaccgg accttcgcga
     8161 tcctgctcat caccgtgatc gccgtgtccg gcgtccagct tctgatcgga tgagggaccg
     8221 tgtgccccac aacacagctc cgccgcctcc ggcccgaccg ggtccggacg gtgaggggta
     8281 cgcaggcttc tcggccgccg agatgcgccg ccgccgggag cttacgctgt ccgcgatggt
     8341 cgaggcgggt gtcgaacacc tcgtcgtggc ggggtccaac cggttcggtc ccgcagtgga
     8401 atggctgagc ggctggccca ccacgaccga gtcgatgctg ctcgtcgggc cggaggagcc
     8461 cgacgtgctg ctcatccaac tgcgcaacca cgtgccgaac gcccggtcgc tggccgcggc
     8521 gtgcgacgtg cgatggggcg gccatgtcac agtcgacgcc gccgtagggg agctccggcg
     8581 ccgggggggc ggccgggtcg gggtgatggg gccggtgtcc caccgagccc accggacact
     8641 ggaggaggag ttcgacgagc cggtggacct cgacggcgca tacgtccgga tgcggctggt
     8701 gaagtccgag gaggagatgg cgtgggtccg gtacggcgtg gagctcaccg atcgcgctat
     8761 cgacgcgctc ctcgctcacg gcggacccgg gctcactgtg gaggagctgg tcgacgtcgt
     8821 cgagcgcgcc tacgtcccgc tgggcggcgg cacgcacatt cacttcttcg ccgtcacccc
     8881 gatggacaac ccctcgcaat gcgtgcccgc ccagtggccc tcccgtcgac ggctccgccc
     8941 gggtgacgcg gtgaccacgg aactgagcgc gtcctaccgg ggctacccgg gacaggccct
     9001 ccggacgctc tcggtggctg ccgagccgaa cgcgctccat cgccggctct acgcggtggc
     9061 cgaggaggcg ttcgaggcca tcattgcggc catccggccg ggcgcccatc cccgagatct
     9121 ggtcgaggcg tcctccccga tcgaggacgc cggcatgacg atctacgacg acctcgtgca
     9181 cgggttcgtg ggcggctacc tcccgccggt gctggggagc cggagccgta ccctcggagc
     9241 gatccccgac ctgccgctcg aggaggggat gacggtcgtc gtgcaaccga acgtgatcac
     9301 gccggacgag cgggccggcg tgcagatcgg ggagctggtc agggtcggcc cggcgggggc
     9361 ggagcggctg caccgggccc ctcgcgggtt cctgcggatg ggctgatggg cggccgaggc
     9421 gagccatccc cccagagcga ggtctccatc gggcgggtgt tgttccagcg gaacttcttc
     9481 ccgtacttcc tgggcaacct catctcgaac agcgggacct ggttccagag cgtcgcgcag
     9541 gtgctcttcg tctaccggct gaccggttcc ctgttcatgg tcggagtggt gaacttcgcc
     9601 cagtttgccg ggatgctgat cgtcacgccc gtcggaggga gctacgccga ccggttcgac
     9661 cgccgcaagc tgctgatccg tgtgaacctc gcaaccgcag cccttgccgg gctgctcgcg
     9721 ctcctcaccg cgatggacct gatcacgctc cctatcctct tcacgctcgt cctgctgatc
     9781 ggcacggctg tcgcgctaat caccccaacg atgcaggcgc tgatctccgc gctggtcccc
     9841 cggcaccagc tgcccaaggc tatcgccatg aacggggtga ccttccagac cgcccgggtg
     9901 ctggggccga tcctgggagc cctggtggtc gaccggttcg gcttcgcgtg ggcgttttca
     9961 gtaaacgcct tgtcgttcgt cgccctggcg gcgagcgtat gggcggtgca ccccatcacc
    10021 ccgcaagccc gcgcgaaggg acggccgccg atgtgggagg gcctccgggt ggcccgcgag
    10081 gatcgggcac tcgcggcggt gctaagcgtg ggcatcgcgg tgtcgttcgc catggacccg
    10141 ctgatcacca ccggggcgga gttcgccacg gaggagttcg gccgcgccga caccctggtc
    10201 gggctactca tcgcggtcta cggggccggg gccatcgtgg cggcattcac ccgatcgtgg
    10261 gaagagtcca cctcgccccg acgggtgggg gcgagcgtct cgatgatcgc ggcggggatg
    10321 gtcgccttct ccctggcccc cacactcgcg gtcgcactcc ccgtgctgtt catagccggg
    10381 tacgggcacc tatcgtcgac ggccgcatct ctggcgaccg tacaactggc cgtcccggac
    10441 gagatgcggg gtcggatcat ggcgctgtgg acgatcacgt tcctcgggat gcggcccctg
    10501 gccagcctgc ttgatgggac actcgcctcg gtagccgggg tccggtgggc ggcggcggcg
    10561 atgtcggtcc ccgccgtgct ggtggccatg ctccttttct ccacgaaccg tggcacgcgg
    10621 gaggacggct gatcgggggc cggccgctca ctgctggtag ggggcttgta ggtattcgat
    10681 atcatatcgc gacgctgctg cctgggaacg tcgctctacg aggggagagc tgatgaaggt
    10741 cgacctggtc ataaggggcg gttcggtgtg gcaagagggc cagggattca tcaacgccga
    10801 cgtcgccgtg gacggcgagc ggatcgtcgc gatcggcgcc cccgatgccg ggttcccgga
    10861 gggccgccgg gtggtagacg ctggagggct cacggtgatc cccggcctca tcgataccca
    10921 tattcacctg cgtgaccccg ggttcactta caaggaggac tacgagaccg ggacccgggc
    10981 gtccgccaag ggtggcgtga ccatggtcgt ggacatgccc aacacgaacc ccgtgccgaa
    11041 cacgatcgag cggttcatgg agcaccggga gatcgcctcg ggcaagtcgc tcgtggactt
    11101 caaccactgg gcggcgccga ccatcctcga ccagatcgcg cccatcgtgg ccgagggcgc
    11161 cgcgggattc aaggtgttca tgatcagcca ccactacccg tacgacaacc ccgaccagtt
    11221 catcccgctc gacgaccccc tgcgctggca cgacaccatg acggagatcg cgcggacgcg
    11281 tcggccgctg atagtccacc cccacaacca gaacatgtgg atgaccctgg ccgacaagta
    11341 cgaggtcgag ggacggacca cccccgagga ccgcgaagag gcctacatct tcgccgacaa
    11401 cttcattcaa acgtcgtccg tggcttccct gctgttgctg gccaggagca cgggcgtgga
    11461 cctccggatc ctgcataaca actggatgcc gctgctcgag atggtccgga tgttcaaggc
    11521 cagcggctat cgagcgatct tcgagcagaa tccgtgggcg gtgttcggct tcggcgtgaa
    11581 ggaccaggtg aaggacgcgg acgaggtctg gagaaccctg aacgacggca cgatcgacat
    11641 catcgcgagc gaccacgctc cgcattcccc cgaggaggtc gcaaccgctc agatcaacgc
    11701 gttccggtcg gtgatcgtct cgatcacgct cgaggagcac atgctcgcga tgtacctgac
    11761 ggagatcaac gaccaagggc gcatcacgct cgaccggctc gtgcatctgt tctccaccaa
    11821 cgtcgccaag cacctcggcg tgtacccgaa gaaggggacc ctccgggtcg gctcggacgc
    11881 cgacatcacg ctcatcgaca tggagaagga agacgtgatc gaccaggccc ggttggagtc
    11941 caagtgcggc atcaccccct acgagggctg gaagctgaag ggtctcccgg tcgggacgat
    12001 cgtgcggggc cagttcgtga tggaggatgg gacggtgacc ggcgatccgg gctacgggcg
    12061 gttcacggca cccctcgggc cggggaccct caggtagccg tgggccggta cggggtcctg
    12121 ctgccgcact tcggaccgca cgcctcccgt gacacggtcg cacgcgaggc tgtccggttg
    12181 gaggagctcg ggttcgactc ggtgtgggcg cgagaccacg tggtctacga accgcacgaa
    12241 tacgagcacc cggacacgac tttccttgac cccatcgtgg tgctgtcggg ggtggccgcg
    12301 gtcacctccc acatcggcat cgggaccggg accctgatcc cccaccgcca cccggtggcg
    12361 gccgccctgg ccctgtcgtc gctcgacgcc ctagcggggc ccggccggct cacggtcggg
    12421 atcgggctcg ggtcgttcgg ccacgagttc gacgccgtgg ggatgaaggg ctgggaccgg
    12481 cgggaggtcg tggaggagca agtcggcatc ttccgacgct tgtggacggg cgagccggtt
    12541 acccaccacg gcaggttcta ctccttcgag gaggtggcca tcagcccggt cccgaaggcg
    12601 cgggacctgc ccatctggta ctcgggcacg agcaaggcgg ccgtccgccg ggccgtcgag
    12661 tacggcgacg gatggctccc cggttccctc cctcgggccg agtacgggga ccggcggcaa
    12721 cggatggaag ccctggcgga ggcggccgga cgggcggttc cggacagcgg gctgatcccc
    12781 ttcgtgagtc caggacggac ggtggaggag gccgtggcgg cggtaccgat ggaggcgatc
    12841 ttggcgatgg cgaacaagcg ttaccggcgg cccgatgggt tcgcaacgct ggaggacctc
    12901 gacgggaccc tcatggcggg gccgcccgag cgtctgtccg aggagatccg gcggtacgag
    12961 gagctcgggg cgggcacgtt cgtcctggac ctccgattcc ggttcgacga ctggcgggag
    13021 tgcacggagc tgctggcctc cgagctcctc cctcaggtcc ggtcggggga ttaggcgcgc
    13081 ccggcgtccc cgccccgggt cggcgtgtcc ccgaccgcca actgcaccgc ccggacgatg
    13141 ttctcataac cggtgcaccg gcagaggttg ccgcctaccc gctcccggat ccgccgctca
    13201 tcgggcgcgg cctcctccgc caggagctcc gcgacgacca tcagcatgcc cggggtgcag
    13261 aagccacatt gcacggcgtg ggcgacgttg aaggcgcgct ggatcggatg gagctgctcg
    13321 tccccggcca ggccctcgac cgtgtccacg acacggccat ccgcctgcaa cgcgaggacc
    13381 aggcacgacc gcaccgaaga gccgtccagc agcactgtgc aggctccgca gaccccgtgc
    13441 tcgcagccta ccttcgtacc ggtcaagccg agccgctcgc ggagcaggtc ggcaagggtg
    13501 tctctcggct ccaccgacgc ctccgccagg gaaccgttca cggtgaggcg catggcgacg
    13561 ccggtcacca ggccgccctc ccgccgatcc ggtcccgggc cgcgcgtagg gcccgtccgg
    13621 cgagggtccc cgccaggtgg cggcggtagc cagcgctgaa gctccggtct ccgggcgggt
    13681 ccacctcgtc cctgaccgcg gcgatggcct cgtcgacggc gtcgtcggtc ggcgccctgc
    13741 cgagcatgac ccgctcggcc gcatcggcgc gcatcggggt ggacccgccg ttcagcacgg
    13801 cgacccgggc gtggtggacg gtgccgacgg ggtcggtccc cagtacggcg gcgacgccca
    13861 cgatcgcgga ggccccggaa cgaagagcga gctcgcggaa ggctgcgccc gccggccctg
    13921 cgggcagacg gagcctgacc tcggtgagca gctcgccggg agcgagcgct gcggcgaacg
    13981 gcgcgggata gaggtccacg ccccggatcg tccgggagcc ctccggcccg gtggcgtcca
    14041 cctccccgtc gagggcgagc agcaggcatg gccactccgc cccggggtcg gcgttcgcga
    14101 tgctgccgcc gaccgtgccc cggtttcgca cggggacgct gcccacgtgg cccgctgcct
    14161 cgccgatcgc ggggcaccgg cgcaacagct cggggtggcg ttccacggcc cgctggcggg
    14221 ccatagcgcc gatgacgacg gtgccatgcc cggccgcctc gagggcgtcg aggcccggga
    14281 ccagcccgag gtcgaccagc atggtgggcc gcaccatccc caaggccatg gcgggcagga
    14341 ggctctgccc gccggcgagg ggttgtgcgt cgggagcgcc agccgcgagg gcctccacgg
    14401 cctcgccgag gctggtcggg gcgacgtagg agaaggcggg gagcttcatc gtggacccgg
    14461 cgccggcgtg gtcgcccgtt gaccagaacg actgggggct agccggaccg gggcaccacc
    14521 gcccaggcct cgatctctat cagcatatcc ggcaggtcga tgccgacccc ccgaagcagc
    14581 gtggccggcc tgggatgctc gcggagatcg ggctcgtgct cctcgaagaa ggcgtacatt
    14641 tcgtctcgca tgtcgaacac ctgctctcgg ttcttcacgt agtaccggaa catcacgatg
    14701 cttttgaggg acgcccccat gatctcgagg ttgtccttga tctcctgcag gcactgccgg
    14761 gtctgagccc gcatcccacc ctggaccacg ttcccccgcc cggcgcgttc ctcctcgggg
    14821 gtccgggccg gcgcgtcgtt gaacgggtcg cggccggtag tccccgacag gaacaggatg
    14881 tccacgtcgc ccctcgtgcg gaagccggtc gcccagttca tcttctcctt gtggtaggcc
    14941 ggccatagtg tgccccagtc gtactcgacc ttgcccccga cctgcaggcg gagcgcctcc
    15001 gggttcgtcg acacggatca ccctcccggg aaaagtctct tgaagtcaag atgctatcat
    15061 gcgcgacccg gcccgagttc cgccggcccg ccgggaagga gacgcacatc tcgccgcgtg
    15121 cgaccacacg accggggttc cgcatcgccg tcgacatcgg agggacgttc accgacctcg
    15181 tcgctgtcgg caccggacgg gtggtcgcgg cggcgaaggt cctgaccacc cccgccgacc
    15241 cctccgaggc cgtcgaaagg ggggtgggcg agatcctgga cggcatcgac ccggccgggg
    15301 tcggcgaggt ggtgcacggg acaaccctgg tatcgaactc gctgatcgag cggaagggag
    15361 cccggacggc gctcctcacc actaaggggt tccgcgacgt cctcacgatt cgccgcgagc
    15421 tccgttacga cctgtatgac ctgttcctcg agatgcctga gccgctcgtg ccccgccgcc
    15481 tgcgttggga ggtcgacgag cggatcctgg cggacggctc cgtagaccgg ccggtcgacc
    15541 ccgaggacgt ccggaggatc gcccgacggc tggagcgcga gggggtgcag gccgccgcga
    15601 tctctctgct gcacagctac cgggatccgt gtcacgagca gacggttgcg gcagcgctcc
    15661 gcgaggtcct cccggacctc cccgtctccc tgtccagcga ggttgcgccg gagctgggcg
    15721 agtttcaccg gacaagcacc gtcgttgcga acgcgtacgt gatgcccctg gtggcgaggt
    15781 accttgagag gctgcgggag cggctggccg cgatgggact tcgctcgccg ctgcacatca
    15841 tgctgtcgac cggcggcggc gggctcgccg gggtcgatac ggccaagcgc ttcccggtgc
    15901 ggttgctcga gtccggaccc gcggcgggcg ccctctcggc cgggttctgg gggatccgga
    15961 ccggtcaccg cgacgtcctg gggtttgaca tgggcggaac caccgccaag gccgtgctca
    16021 tcgaggacgg tctcccgcta gtgacccggg agcacgaggc cgcacgggtg taccgcttca
    16081 ccaagggcag cggcctgccg ttgaaggtgc ccgtgatcga tctgatcgag atcggagccg
    16141 gcggggggtc gatcgcccgg gtcggcccgt tcgggctccc caaggtcggg ccggagagcg
    16201 cctcgtccga ccccgggccg gtctgctacg gcctcggcgg gaaagaaccg actgtgaccg
    16261 acgccgacct cgtgctgggc ttcctgaatc ccgcgttctt tctgggcggc gacatggcgc
    16321 tcgacctcga cgccgcccga gactcgctca gtcgccttgg cgacgagctc ggactcccgg
    16381 tcgaggggac cgccgcggcg gtgcaccggg ttgtgaacga gaacatggcc agcgccgcgc
    16441 ggatgcacgc gatcgagcgc gggcgcgaca ttcgccgctt cgccatcgtg gcgacgggcg
    16501 gcgcgggacc ggtccacgcc tggggggtcg cccgggggat cggcattcgg accctggtgt
    16561 tcccgccgag cgccggtgtg gcgtcggcgt tcggcatgct gaccgcgaac cccggtttcg
    16621 agttcgctcg gagcctgccc tgcgccctgg gcgatgtccg gtggggggaa gtccgggcgg
    16681 cgatcgccac gatggcacgc gaggggaccc gccagcttcg ccaggcgggc gtccggcgcg
    16741 atgacgttgc gatcaccctg gccgtcgacg tccgccaccg cggccagggc gacccggtca
    16801 ccgtcgagct cggcgacgcc ctgcaccgga aggaccccgc ggtgcaggtc gaggaagcct
    16861 tcgaggcaac gtacgtccgc agctacggcc gccggccgcc aggcgtcgag cccgagatca
    16921 tgacgtggcg ggtccgggtc atgggaccgg agccgcagct cgacgtccgt gctcgccgac
    16981 gggccgacac ggcgcgtccg gcgctgaagg gccggcgccg cgtctggttc ctggagtcgg
    17041 ggcgcttcgt ctcggcgaac gtgtacgacc gctacgccct aggtccgggc gatgtcgtga
    17101 aggggcccgc ggtcgtcgag gagcgcgagt cgaccgcggt gatcggcccg gggtcgactg
    17161 ggcgggtgga cgaggtcggt accctggtgg tgacggtgcg atgaccactg gccgtgaagg
    17221 ctctgacggc gcccgacggc gcccggcgat ggacccagtg accctggaga tcttgtggac
    17281 ccgcctggtc accgtgacga acgagcaggc tgcagcgctc cagcggacgt cgttcacgcc
    17341 gatcgtgcgg gagtccggcg acctgtctgc cgccgtgttc gaccgccggg gtcggatgct
    17401 ggcccaggcc gtgaccggca ccccgggtca catcaattcg ctggcgacct cgatgaccca
    17461 cttcctggcg gcatttccgc cggagacgct gcagcccggc gacgtgctga tcaccaacga
    17521 cccctggatg accagcggcc aactgaacga cctttcggtg gtaacgccgg tcttccaccg
    17581 cggacggctg gtcggcttct tcgggaacac ctgtcacgcc atggacatcg gcggccgtgg
    17641 cctgtcggcc gacgcggggg aggtcttcga ggagggcctg tcgatccccg tgacgaagct
    17701 cttcgacgcg ggccggccga atgaggagct gctccggatt ctccaggcca acgtccgggc
    17761 gccgttcgag gtcctcggtg acgtgcacgc acaggtggct ggcaaccagg tcggcgcgga
    17821 acgcctcctc gagtatctgg aggactttgg gctcgacgac ctcgaggatc tagccgagga
    17881 gatcttgggc cggagcgagc aggccatgag ggatgcgatc cggaccctgc ccgacggcga
    17941 ctaccggcac acgatccaaa ccgacggcct ggacgagccg gtgacgatcg cgtgccgggt
    18001 gagcgtccag ggcgaccgca tccacgtcga ctacagtggc agctcgccgc agatcgaccg
    18061 gggcatgaac gtcgtcctga actacacggc ggcctacacg agctatgcct tgaagtgcgc
    18121 gatctcgccg gaggtcccgc acaacgatgg ctcgttccgg ccggtctcca ccagcgcccc
    18181 cgaggggtcg attctgaacg ctctgttccc cgctgcggtc gccgcgcggc acatcgtggg
    18241 gcacttcctt ccccacgcgg tcttcggcgc gctggtccag atcgtcccag accgtgtgct
    18301 ggcggagggc gccggcaaca tctggctgac gaccgtacgg ggcgcgggcg ccgaccgctt
    18361 catcactgtg ttcttcgccg cgggtggcat gggcgcccgt cccaccgccg acgggctgtc
    18421 cacgacgtcg ttcccgagcg gcatcgccac cgcgcccgtc gaggtcatcg agtcgacgtc
    18481 gccactggtg ttccggagga aggagctccg ccgggactcg ggagggccgg gtcggttccg
    18541 gggaggcttg ggccagcgga tcgaggtgga ggtccggacg ggcgagccgt tcatcgtctc
    18601 gtcgctggcc gaccggatgc gcatccgggc ggaggggtac ctcggcgggc ttcccggcgg
    18661 cgcgggcggg ttctcgacct ccctcgggac caagccgaac ccgaagctct cgctgccgct
    18721 tcaggcagga acccgcttca cgctcgacct ccccggcggc gggggcttcc accccccggt
    18781 ccagcgcaac cccgccgcag tcgcccggga tgtcgccgag gggctggtca gtccgacctc
    18841 cgccaagcgg gactacggcg tgagcgtgac gccgtcgggg cgggtgaagg ggaccgtcag
    18901 ccggcgcgga cgggggcgaa gaggtcgatg agccgcctcg cctggtgggg ggcagccgga
    18961 cggaggatgg ggacctgcac gccgacgtcg cggtaccgct ggaccctcgc cacgacctca
    19021 tcaggcttcc cgcttgcggt catcccctcg atgtaggagt cggggaccac gcggatcagc
    19081 tcctccttgc cccctcgtgc ccaggcctcc tggaatatcg ggacgtcggc ctcgtgcatg
    19141 tacggctcgc ccaccagctt cttcggcccc atgatgaacg gcagctggat ggggtcgagc
    19201 ttgctggcaa cctcccagcg gatcgcgtcg agcgcggcca cgcggtcgtc ctcgatcgag
    19261 cagttgacga gctggtagat cgcgatcgcg tcagggtccc ggccggcctc agtcgccgcc
    19321 tcgcggacga tcgcgatcgc attctcggag tactccggcg agcagaccgc gttcagcacg
    19381 acgccgtcac cgatctcccc cgcgatgcgc aggcccttcc ggctggttgc cgggatgaag
    19441 atggggatgt cctggcgggg cggggtccat ccaagccgga cgtcgtcgaa gtgcaccacc
    19501 tttccgcggt acgagaccgt ctccccggtg aggatcgccc ggatcgcttc gacccactcc
    19561 cggagggtca ccccgggatc gaggtgctcc agcccgtggc gtttggcgtg cgtggaggtg
    19621 cacgccccgg gggccaggag aatccgtcct cccgtcacct cgtcgaggga cgcgagcgtt
    19681 tgggccatca ccagggggct gcgcaggcgc acgatctgcg tgcatccgat cactaggcgg
    19741 tcggtggcca aaccgatagt ggcaagggcc gagaccgagt cgcgcatcag ttcgatcgtc
    19801 tcggagaaga agccgatctc gaagccctgc tcctccgccg cgcgcatcca ccgtgccatg
    19861 tcgcgcagac ccatcgcggg gtcgtaccct gcggcgaacc ccgtccgttc catcggccgg
    19921 atgaggttat catgtatctt aacgcctaga tatctcgggc ggccccgggg gagggatcgg
    19981 atgggggcct cgggagcggc ggaccatgtt gaccagatcc tcgcggagtg gcggcgggag
    20041 cgtccggatc tcgacttctc ggccatggcg gtgatcgcgc ggatttcccg cctgtcccgc
    20101 atcctcgagc ggcggatcga gacggtcctc tcccggtacg gcatcaacga gtccgggttc
    20161 ggcgtcctcg cggcgctgcg acgggcgggc cccccgtacc ggctcacccc caccggcctc
    20221 tacaactcgc tgctgatctc gtccggggcg atgacgaacc ggctcgaccg gctgaccgcg
    20281 gcggacctgg tcgagcggat ccccgacctc gacgaccggc gcagcgtgct ggtgcggctc
    20341 accactcgcg ggctgcgtgt ggtggaggag gccctcgacg cgcacatcga gaacgaaatc
    20401 gcactccttg gcgcgctgtc agcccgggac aagcaggagc tggccggcat cctccgcagg
    20461 ctgctcgttg ggttcgagtc cgacgcggga ccccacgcgg tgctcgacga ggacgacgcg
    20521 ccggggcggg acggcctcag gcgacgctcc agcctccatc cacccggaga tgctgccccg
    20581 tgatgtacga cgccgctggc gaggccagga acacgaccgc gcccgcgacg tcatccggcg
    20641 tgcccactcg ccgcagaggg gtgcgctcct cgacatagga gcggttttcg gggcggtcga
    20701 gccactcctc gttcagcgag gtccggatgt agcccgggcc gacggcgttc acccggaccg
    20761 cgggcgcgaa ccgcacggcg agtgcccggg tgatctggat cagcgcggcc ttgctggcct
    20821 ggtaggtgac ggacgcgccg gtgacccgtt cgcccgcgag cgacgcgatg ctaacgacgg
    20881 cgccctcccc ccgctcgaac atccgcgcgc ccaccgcctg gctgaggaag tacgtccccc
    20941 ggacgttcgt cgcgaaaacc gggtcccagt gggatggccg aatgtcggga ggctgcgtgc
    21001 ggtcgatcac cccggccgcg ttcacgagaa cgtcgacccg tcctcgccag cgccaggcct
    21061 ctgccacgac ctccggcagg cggtccacat gagcgatgtc agcgggcagg gccaaagcct
    21121 ccggccccag agcgcgcacg tctctggcca cctcctcgag ctgacctccc gtgcgggcca
    21181 ccgcgatgat gtccgagccg gcccgggcca gttcgagcgc gatggcacgt ccgatcccac
    21241 gccccgcccc tgtgaccagc gccgtgcggc cggacaaggc aacgctccac atcaccgccc
    21301 tcctccccgt atgaaccgct gcagcgagcg ggccacttcc aacagccggt cgtcccgcac
    21361 agccgacccg gcaagctgga taccctccgg gatgccatcg ccgtccgccc cgccggcggg
    21421 cgggacctcg gaaatctcgg cgagggagag cggcgtggtg gtccggagca gcgcgccgtc
    21481 gccccatacc ggagcggctg ctgcgtcgac cagctcggcc ttcgcgggca gcgccgccac
    21541 ccacatggcc cgggcaacca ggacatcgga ctcgtccatc atcccgcaca ttgctgcggg
    21601 cacatcgcgt cgaagccgct cggccgactc ggctttcccg gcatgatgtc gtcgccatcg
    21661 gcgcagggcc gcttgcgtcg atgacgcata ccggagcgtc ccatccttcc tcgtgacaga
    21721 cccgggccgc ctccaggacg tcgagggcga cgaacgcgct tctgcgccgc cgcggcgccg
    21781 ggcgagcgcc tgaccacggc ggcgcccacc tcggccagcg cctccaaggc ggcggcggag
    21841 caccgggtca cgtccgctcg gcctcgtgcg cagtctcggc caggccggcg aacggggcgc
    21901 gaccgaccct gaggcggcga ccgccccgcg tgatcggttc ggcggcccga tctggacgca
    21961 gcgcggaaga gccgtcgcgg accccgaacg catcgaacag gaccctgcat gccttgaccc
    22021 acatggcgat ggagcccgcg tcgtccgtgg acgaagcgag ggacacaacg tcgctgggca
    22081 tgacctggtc ggggttcaga tgcggacccg ccagaccgca gagcgcggac agcgagcgga
    22141 tggaccagcc cgtgtcgacg cagaactcgg ccgggaccgc gcccgcctcg accgcgggcg
    22201 gctgcgccgc cgctggagcc gcccggggtt cgaccgggcc caccgggttc aagcgtgctc
    22261 cacgtcaccg aaccgttggg aactcgccca tgcgggtctt agccgtcacg ctcgcgtccg
    22321 cgcaacggag cactgcaacc gcccgcgccc cgccggacgc ccggcccggc ggcctggcga
    22381 cggagccagc ccgggtgggt atctcggcca tgtcgatctt cctccacgcg gagggcacgc
    22441 cctgcagcgg tcccgcgggc cgaacgaagc ggctgcggcc cgggctcgct cgttggcgcc
    22501 ctcggcgact gccacaatgt cgactttcgg gtcgaggtcc tcgatccgag ggtcgggacc
    22561 tcatccatca ccctggccgc actgaggacc ccggcgcgga ggtctccgac ccagcccccc
    22621 ggcccggtgc gcacgaacga gccgaccagg tgcttcaccg gaatccgtcc tccccgccct
    22681 gcattccctt ctagccgtcg cagcgctggg cgccgtcagg gtaccgcgac gcggctatcg
    22741 tacgtcaccc atgtcagcgg tcatcgacgc ccactcgcac ctgttcccgc ccggcttcat
    22801 tgagcggctg cgggcccggc cgacggcgcc tcgtatcacg gggggaccgg acggggaccg
    22861 gctgatcgtc ttcgcgtccg aggaacgcac cggcgggcgg ccggtcggcc ggtccttcat
    22921 cgatccggcg gagaagttgc ggttcatgga ccggttgggg atcgaccgtg cggcggtcca
    22981 gatggccaac cccctgctgg agccgttcga tgggcaggag gcggtcgacg cggccgcgga
    23041 gctgaacgcc gagatggccg gcctcctcag ggcgagcggc ggccgactgg cgggcctcgg
    23101 cgcgctcccc cagcatgacc tggccgcggc ggcggctgcc gccgagcagg tggcggcgac
    23161 ggacaccctg ttcgggatcg cgtcaggccc cctgctctgc cgccggcaac tcgacgaccc
    23221 cgagctggac ccgctgtggg gcctgctgga acggctcgac gtgccggtaa cgatccaccc
    23281 ccagcggggt ctcccggtcc cagagctgga ggggcacggg ctggccttgt ccctgggcct
    23341 gtccctcccg ttcgagacga cggtggccgt ggcccggctg gttctcgggg gagtcctgca
    23401 gcggttcccg ggcctgcgga tcctcgccgc gcacgggggc ggcgccctgc cgttcctggc
    23461 cgaccgtctc gacgccgtct ggcgcaccga cccgcgtgct cgggagcaga tgcccctccc
    23521 gccatcgacc gaccttggcc ggctatggct ggacggcctc gtctacgggc ccgcgccgct
    23581 gcgtgcggcc ctggctctag cgggaccgga gcgcgtgcta ttcggcacgg accatcccta
    23641 cgtcgtcgcc gacgctgagc ggaacctggc ggcgatcgac gaggcgacgg acggaggcgc
    23701 cgacgcgctg gcggtccggg gcggcgtggc ggagcggttc ttcgggctga cctaaccgcc
    23761 agggcccccg ccctgcgcgc gctggcgcgg ggcctgcata ggtatctcga cgctaagatg
    23821 ggggcgtgag cacggccgac ctccccgcca cgatgcgtcg tcacccggtc cccggagccg
    23881 tggttcggga gtgggtcatc ccggccaagg ggtacgacgc cttcatggtc tccgcgcgtg
    23941 acgtcgtccg gttcgtggac gttgagggca agcaggtccc cgacgtgatc tgctacgacc
    24001 cctccgatcc cacagatgag ctcagcatgg ggaactccct cttgctcaac aagcggcgcg
    24061 aactccgtcg aggcgacgtt ctgtactcag tcacctgcca caagatgatg acgatcgtcg
    24121 gctactccaa cgaggagagc ttcgcgtacg gcccgatgtg tagcgaagag gtgaaccagc
    24181 tccgctacag ggtcccgggc acggcgaact gccgggacaa cttcgcgatg gccctggccg
    24241 cggctggcat gtcagtggac cgccgccgaa tccctgacgc gttcgtcccg tttatgcggg
    24301 tggaagtgga cgaggatggg accttggaga tccgggaacc cacgagcgag ccgggcgacc
    24361 actatgacct ccgggcggag atgaacctgc tccttgcagt ctcgaactgc ccgcagaagc
    24421 ggaacgcgtg caacggtttc aaccccacgc cgatgggtgt catcgtctac cggccggacg
    24481 ccgccgcgcc agaacctccc ggggccggca gggggattcg cacggacagc tcggcgggct
    24541 taccacggcg atagggaccg ccggcggcga tatcagcggc gtcgacctcg tcgagcacgg
    24601 ggcgcgagtc ttgtcacaga tcgcggtgac tgcggggatg aggcgcacgg tgccgtgatc
    24661 gtcgcgcaag cgctagtgtc caccgggcag atcaacatca tggtcgacga cggcgtccat
    24721 gtgtgtcctc gcgacggtca ggcgccgatc gctgtgagga actcgtcgag gtccggggcc
    24781 acgtggtaac tcggggcaac gtggccttcc aacgcctcga tcgtcttgta gccgttgccg
    24841 gtcacgtagg cgacggtctc ctcgtcggga cggatggctc cagactcggc cagtcgtttg
    24901 aggaccgcga tcgtcacgcc tcccgccgtt tcggtgaaca caccttcggt ctcggccagc
    24961 aggcggatcc cgtccacgac ctcggcctcc gtgcaggcgt cgatcacccc gccgctctgg
    25021 ttg
//
LOCUS       DASUTW010000128         4318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_168592, whole genome shotgun sequence.
ACCESSION   DASUTW010000128 DASUTW010000000
VERSION     DASUTW010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168592"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1252)
                     /gene="dprA"
                     /locus_tag="VF058_04305"
     CDS             complement(71..1252)
                     /gene="dprA"
                     /locus_tag="VF058_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX6207563.1"
                     /translation="MPPDRASPAPDEEVEARPGRAGAGDARSLPRGWPEGFAASEGDR
                     EALLRLSTMNVVPAELYARAWMEGSAAACLAATREPRSRGPRPTPGEILARLEACGGR
                     FLVVGDPGFPERLLDLPDPPAWLFVRGSVPLDVLAVAIIGARRCSSYGRETAEAIGRG
                     LAALGVAVVSGAALGIDGAGHRGALEGEGHTIAVLGSGVDVPYPPSNRDLIERIAVEG
                     ALISEYPPGTPANGFRFPARNRLIAALARSVVVVEGAAGSGSMITAEIATDVGREVYA
                     VPGNVTSRLAEVPNALIRDGAPMVRHAQDVLDCLRGEVSHALRALRDGDRGPPPDEEG
                     LNAEERRVLGALAAPATAEGVAGDAGLPLHRVLPVLAALELRGLVRDVGGRFERRSGE
                     A"
     gene            complement(1242..2774)
                     /locus_tag="VF058_04310"
     CDS             complement(1242..2774)
                     /locus_tag="VF058_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX6207564.1"
                     /translation="MYARAVGVAVVGVHGHPVSVEAHIGRGLPALQLTGLPGAAVQDA
                     RERVRPAVENSGLEWPLRRVTVNLSPADLRKEGPGFDLPIALAVLAATAQLDQERLPG
                     YAAAGELSLKGSLVPTPGILAVAWAARETGLRGVIVPEANAAEAALVEGIEVVGAPSL
                     ARAVEFLRGRWEPGPPSEPPPGSVDGVGVDFSEVRGQAEARRALEIAAAGGHNVLLFG
                     PPGGGKTMLARRLPTILPAMSRDEALQVTRLHSIAGLVPPGRGLIEARPFRAPHHSVS
                     TAGLLGGGSHVIRPGELSLAHHGVLFLDEVTEFRRDVLEGLRQPLEDGRVVVVRASGA
                     VAFPARCTLVAAANPCPCGYQGDPRRQCTCSEARAAQYKHRLSGPLLDRLDIRLTVPR
                     LSKAELMGAAAGEPSATIRDRVEEARERQRARLRGTPFGANAAMPGSVARRHARMTSS
                     AERVLGSAVDRLGLSGRGFDRAMKVARTIADLEGSERVTDAHVYEALRFRGADREGVG
                     AA"
     gene            complement(2809..3654)
                     /locus_tag="VF058_04315"
     CDS             complement(2809..3654)
                     /locus_tag="VF058_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX6207565.1"
                     /translation="MSYGATSATAPLKRVLVRPPMETAGWEAYGWRSEPDIDRMVREH
                     EAYVAELEAAGAEVVAGTTRVPGDPDAIYPCDVAIVTERGAIVLRPGKAARRGEQDAI
                     AADFEAAGVPIVGRLEEPATAEGGDLLWLDERTLVVGRSYRTNDEGIAALRELLPEVE
                     VVSVDLPHHHGAGVILHLLSLISMLDEDLAVAHPPLLPVRLMEMLGERRVRLIEVPEE
                     ELDSQGPNVLALGPRVALALEGSPVTRRRMEAAGVDVRVYAGEDLCRKGDGGPTCLAQ
                     PILRG"
     gene            3727..>4318
                     /locus_tag="VF058_04320"
     CDS             3727..>4318
                     /locus_tag="VF058_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207566.1"
                     /translation="MSIAQGPSWGGNRMRNVLAGTAILELVLGVGFLIAGRSASAGAA
                     GLTVTGAILAATGLLLLLWARSVGRSQRRARQIVAAGLPGTATIESFRQTGVVLNEIN
                     PQVEMDLEVRVEGRDPYMVKKKEFIPGAMVGILTSGAALPVRVDPSDLSRVVILWGAQ
                     DASAAQGMDPAELQHQIDERRAKVRENPLPATARFRK"
BASE COUNT          564 a         1750 c         1498 g          506 t
ORIGIN      
        1 cgcgtccacc cgcgcctcac gtcggggacc ttcgcgaccg cggggacgac gtctggaggg
       61 cgctccgctt ctaggcctct cccgaccgcc gctcgaaccg gcctcccacg tcgcgcacga
      121 gcccccgaag ctccagggcg gccagcacgg ggagcacgcg gtgcaggggg agcccggcgt
      181 cgccggcgac cccctcggcc gtggcagggg cggccagcgc ccccaggacg cgtcgctcct
      241 cggcgttcag cccctcctcg tccggcggcg gcccccggtc gccgtcccgg agcgctcgga
      301 gggcgtgcga gacctccccc cggaggcagt ccaggacgtc ctgggcgtgc cgcaccatgg
      361 gcgcgccgtc ccggatcagg gcgttgggga cctccgccag ccgcgaggtg acgttcccgg
      421 gcacggcgta gacctccctc cccacgtcgg tcgcgatctc ggcggtgatc atcgagccgc
      481 tgccggccgc cccctccacc acgacgacag acctggccag ggcggcgatc aggcggttcc
      541 gcgccgggaa gcggaacccg ttggccggcg tgccgggcgg gtactcgctg atcagcgcgc
      601 cctcgacggc gatgcgctcg atcaggtccc ggttcgaggg cgggtagggg acgtccaccc
      661 ccgagcccag caccgcgatc gtgtggccct cgccctccag ggctcctcgg tgcccggcgc
      721 cgtcgatccc cagggcggcg ccgctcacca ccgccacgcc gagggcggcg agcccccgcc
      781 cgatcgcctc ggcggtctcc ctcccgtagg aggagcagcg ccgagccccg atgatcgcca
      841 ccgcgaggac gtccagcggg acggagcccc ggacgaacag ccacgccggc gggtcgggca
      901 gatcgaggag ccgctcgggg aacccggggt cgccgacgac gaggaatcgc ccgccgcacg
      961 cctcgagccg ggccaggatc tcccccgggg tcgggcgcgg cccccggctc cgcggctccc
     1021 gggtcgcggc gagacacgcg gccgcgctcc cctccatcca ggccctggcg tacagctcag
     1081 cggggacgac gttcatcgtc gagaggcgaa ggagcgcctc ccggtctccc tccgacgcgg
     1141 cgaagccctc cggccacccc cgagggaggg agcgcgcgtc gcccgcgccc gcccgccccg
     1201 ggcgggcctc gacctcctcg tcgggcgcgg gcgacgcgcg atcaggcggc accaacgccc
     1261 tcccgatcgg ccccccggaa ccgaagcgcc tcgtagacgt gcgcgtcggt cacgcgctcg
     1321 gatccctcga ggtcggcgat cgtccgggcc accttcatcg cccggtcgaa gccccgcccc
     1381 gacagcccca ggcggtccac cgcgctcccc agcacccgct cggcggacga ggtcatccgg
     1441 gcatgccgcc gggccaccga gccgggcatc gccgcgttcg ccccgaacgg cgtcccgcgc
     1501 agccgggccc gctgccgctc gcgcgcctcc tcgacccgat cgcggatcgt cgcggagggc
     1561 tctcccgccg ccgctcccat cagctcggcc ttcgagaggc gcggaaccgt gaggcggatg
     1621 tccagacggt cgagcagcgg tcccgacagg cgatgcttgt actgagccgc cctcgcctcc
     1681 gagcacgtgc actgtctccg ggggtcgccc tggtacccgc aggggcacgg gttcgccgcc
     1741 gcgacgagcg tgcagcgcgc agggaacgcc accgcgcccg acgccctgac caccacaacc
     1801 cgcccgtcct ccagcggctg cctgaggccc tccagcacgt ctcgtcggaa ctcggtcacc
     1861 tcgtccagga acaggactcc gtgatgggcg aggctgagct ctccggggcg gatcacgtgc
     1921 gatccgcccc ccagcagccc cgcggtcgag accgaatggt gcggcgcccg gaacgggcgt
     1981 gcctcgatca gcccgcggcc cggcgggacc agtccggcga tcgagtggag gcgcgtcacc
     2041 tggagggcct cgtcgcgact catcgcgggc aggatcgtgg ggaggcgccg ggccagcatc
     2101 gtcttccccc caccgggcgg gccgaacagc agaacgttgt gcccgccggc cgcggcgatc
     2161 tccagggccc gccgggcctc cgcctggcct cgcacctccg agaagtccac cccgaccccg
     2221 tccacggagc cgggaggggg ctcggatggc ggccccggct cccacctccc ccgcaggaac
     2281 tcgacggccc gcgccagcga cggcgccccc acgacctcga tcccctccac gagcgcggcc
     2341 tcggctgcgt tggcctcggg gacgatcacg ccccgaagcc cagtctcccg agccgcccag
     2401 gccaccgcca ggatgcccgg cgtcgggacg agcgacccct tcagcgagag ctcgcccgcc
     2461 gccgcgtacc ccggcaggcg ctcctggtcg agctgggccg tcgcggccag gaccgccagg
     2521 gcgatgggaa ggtcgaaccc cggcccctcc ttccggaggt cggcggggct gaggttcacg
     2581 gtcacccggc ggagcggcca ctcgaggccg gagttctcca ccgcggggcg cacccgctcc
     2641 cgggcgtcct ggaccgcggc cccgggcagg ccggtcagct ggagcgcggg gagccctcgc
     2701 ccgatgtggg cctcgaccga gaccggatgg ccgtggacgc cgaccaccgc caccccgacc
     2761 gcgcgggcgt acatgcacgg accgtagcgc gggggtccga cacgaacgtc agccccggag
     2821 gatgggctgg gccaggcagg tagggccgcc gtcccccttc ctgcagaggt cctcgccggc
     2881 gtagacgcga acgtcgaccc ccgccgcctc catccgtcgc cgcgtcaccg ggctcccctc
     2941 cagggccagc gccacccggg gccccagtgc cagcacgttg gggccctggg agtcgagctc
     3001 ctcctcgggc acctcgatca gccgtaccct tcgctccccc agcatctcca tcagccgcac
     3061 gggcagcagg ggcgggtggg ccaccgccag gtcctcgtcc agcatgctga tcagcgacag
     3121 gaggtgcagg atcacacccg cgccatggtg gtggggcagg tcgaccgaga ccacctcgac
     3181 ctcgggcagc agctcgcgca gggccgcgat cccctcgtcg ttcgtccggt aggagcgccc
     3241 cacgaccagc gtgcgctcgt ccagccacag gaggtcccct ccctccgcgg tggccggctc
     3301 ctcgagccgg ccgacgatcg gaacgccggc cgcctcgaag tcggccgcga tcgcgtcctg
     3361 ctccccccgc cgcgccgcct tccccggacg caggacgatc gccccgcgct cggtcacgat
     3421 cgccacgtcg cacgggtaga tcgcgtcggg gtcgccgggc acccgcgtcg tcccggccac
     3481 gacctcggcg cccgccgcct ccagctccgc cacgtacgcc tcgtgctccc ggaccatccg
     3541 gtcgatgtcg ggctccgacc tccagccgta cgcttcccag ccggcggtct ccatgggcgg
     3601 ccgcaccagc acccgtttca gcggggccgt ggccgaggtc gctccgtagc tcacgccgcg
     3661 atgatagggc ttgacccggg agcggggcgc ccccacactc ccgacgagca gagggaggga
     3721 gatccgatgt ccatcgcgca gggaccgagc tggggcggca accgcatgcg gaacgtcctc
     3781 gccggcacgg cgatcctcga gctcgtgctc ggcgtggggt tcctgatcgc gggccgctca
     3841 gcgagcgccg gggccgccgg gctgaccgtg acgggcgcca tcctcgccgc caccggtctc
     3901 ctgctcctcc tctgggcccg ctcggtgggc agatcccaga ggcgggcccg acagatcgtc
     3961 gccgccgggc ttcccgggac ggccacgatc gagtccttcc gccagacggg cgtcgtcctc
     4021 aacgagatca acccgcaggt cgagatggac ctcgaggtcc gcgtcgaggg ccgtgacccc
     4081 tacatggtga agaagaagga gttcatcccc ggggcgatgg tggggatcct cacctccggg
     4141 gccgccctgc ccgtgcgggt ggatcccagc gacctctccc gggtggtgat cctgtgggga
     4201 gcccaggacg cctcggccgc ccagggcatg gaccccgccg agctccagca tcagatcgac
     4261 gagcgccggg ccaaggtccg cgagaacccc ctccccgcca cggccaggtt ccgcaagg
//
LOCUS       DASUTW010000129         4366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1206209, whole genome shotgun sequence.
ACCESSION   DASUTW010000129 DASUTW010000000
VERSION     DASUTW010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206209"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /gene="dnaN"
                     /locus_tag="VF058_04325"
     CDS             <1..924
                     /gene="dnaN"
                     /locus_tag="VF058_04325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX6207567.1"
                     /translation="VSEEGSALVPARLVAEMVKSLPPDDVDISADGSIARVTCRSFEG
                     TLRCLPAEDFPQLRHPEGIRLTLEAPAFGEAVAQVVKAASRDEARPILTGVLFEANRE
                     GLTMAATDSYRLAVRELQATGDGEGKALVPERAISEVGRAAAAEEKGEVEVVIGQAQA
                     SFRVGELRLISRLIEGEFPNFRQLIPEPGGNKLVAPRQELLEAVRRVGLLARDNAPVR
                     LELNALGVRLTSSSPDLGDAVEAVEAGYEGEELTVAFNPTYLGDGLAAVTDERVHVEL
                     RDGLKPAILRGEGGDAYTYLVMPVRLPAPVG"
     gene            925..2001
                     /gene="recF"
                     /locus_tag="VF058_04330"
     CDS             925..2001
                     /gene="recF"
                     /locus_tag="VF058_04330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HEX6207568.1"
                     /translation="MQLAWIEVRDFRNHARTRLEVPAGLTAVVGANAQGKTNLLEAAH
                     YLLTLSSPRTAADQPLIREGAGSAFLRGEVETASGRVLVEVEIRPSGANRIQVNRSSV
                     RRKRDLRSRVRSVFFIPEDLAIVGGGPEERRRFLDEAAYGLWPMREGERRAYDRALRQ
                     RNRLLKEHEGPGPPAELEAWDAELIASGALVTAARAAAAERVAPRASERYEGLSGDRL
                     LVAYAPSVAGEDLEEAFREGLAARRGDELIRRSTLVGPHRDDLELAVRGRTARRFASH
                     GEAWAAALCLRLGLTGALEAEVGESPVLLLDDPFSGIDPERRRRLAASLSAGPQTMLA
                     LPDEAQIPDGAVVWEVRDGAATPR"
     gene            2024..2320
                     /locus_tag="VF058_04335"
     CDS             2024..2320
                     /locus_tag="VF058_04335"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEX6207569.1"
                     /translation="MGEILRGLLSEPPLRRGLRLGRLGRVWPRVVGERLAPVTAPVSL
                     DEGGLVVSASTQAWAAQVRFMADEIGRRAGEVLGEDPPPPVRVVVGPERPKRDG"
     gene            2434..>4366
                     /locus_tag="VF058_04340"
     CDS             2434..>4366
                     /locus_tag="VF058_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HEX6207570.1"
                     /translation="MAQKTSKTTKRTRKRGYTAADITVLEGLEAVRKRPGMYIGSTGP
                     RGLHHLVYEVVDNAVDEALAGQCTKIKVVLRKDGSVLVEDNGRGIPVTKIPGQKDRRP
                     AVEVVLTTLHAGGKFDGKSYAVSGGLHGVGVSVVNALSRRLEVEVMRDGHTWTQAYER
                     GKRLSPLKKGKATKKTGTTVVFWPDPDVFEELDFKRELLAERLQELSFLTGGVEIALV
                     DERERKPWEAVFKAKGGLADFVKHINQAKEPIHARVIHFDAKGKDAEVEVAMQWNGSY
                     SESLHSFANTINTHEGGTHEEGFKKALTNVMNRYARAKGLLKEKEENLIGEDIREGLA
                     AILSVKIKDPQFEGQTKTKLGNTPMRSLVETTVNEHFMNWLQENPGPAKRIYQKSEQA
                     AKARLAARQARDLTRRKGLLDSSLPGKLADCQLTDPEVCELFIVEGDSAAGPAIQARD
                     PAYQAILPIRGKILNVEKARLHKILENKEIQALITAIGTGVHDEFNLEDARYHKIVMA
                     TDADVDGAHIRTLVLTFLYREMPELIDAGYVYIAKPPLYKIKSGKQDVYIEKESELEE
                     VLLRDKLEKLEVTDREGEQFSLTHARWQKYGRLLKQYEGWAAALRAEWGHDPITFLEE
                     SAVLDEGVTDADQALAMIKG"
BASE COUNT          714 a         1478 c         1594 g          580 t
ORIGIN      
        1 gtctccgagg aggggagcgc cctcgtgccg gcgaggctcg tcgcggagat ggtgaagtcc
       61 ctgcccccgg acgacgtcga catctcggcg gacggctcga tcgcccgggt gacctgccgc
      121 agcttcgagg gcaccctgcg ctgcctgccg gccgaggact tcccacagct ccgtcacccc
      181 gaggggatcc gcctgaccct agaggccccg gccttcgggg aggccgtggc ccaggtggtg
      241 aaggcggcct cgcgcgacga ggcccgtccg atcctgacgg gcgtgctgtt cgaggcgaac
      301 cgagagggac tcaccatggc tgccacggac tcctaccggc tggctgtccg ggagctccag
      361 gccaccggcg acggcgaggg gaaggcgctc gtccccgagc gggccatcag cgaggtgggc
      421 cgggccgcgg ccgcggagga gaagggcgag gtggaggtcg tgatcgggca ggcgcaggcc
      481 tcgttccggg tcggcgagct ccgcttgatc tcgaggctga tcgagggcga gttcccgaac
      541 ttccgccagc tcatccccga gcccggcgga aacaagctcg tcgccccgag gcaggagctg
      601 ctcgaggcgg tgcgtcgtgt cggcctcctg gcccgcgaca acgcgcccgt gcgcctcgag
      661 ctgaacgcgc tcggcgtgcg cctgacctcc tcctcgcccg acctcggcga tgcggtcgag
      721 gcggtggagg cgggttacga gggcgaggag ctgacggtgg ccttcaaccc gacctacctg
      781 ggggacgggc tcgccgcggt gaccgacgag cgcgtgcacg tggagctgcg ggacgggctg
      841 aagccggcga tccttcgcgg ggagggcggc gacgcctaca cctacctcgt gatgccggtg
      901 cgcctgcccg cccccgtggg ctaggtgcag ctcgcgtgga tcgaggtccg ggacttccgg
      961 aaccacgccc gtacccggct cgaggtgccc gccgggctga ccgccgtggt cggcgcgaac
     1021 gcccagggga agacgaacct cctcgaggcc gcgcactacc tgctgacgct gtcctccccc
     1081 cggacggcag ccgatcagcc cctgatccgg gagggagcgg gatccgcgtt cctgcggggc
     1141 gaggtcgaaa ccgcgagcgg acgtgtgctc gtcgaggtcg agatccgtcc ctccggggcc
     1201 aaccggatcc aggtgaaccg ctcgtccgtc aggaggaagc gcgacctccg gagccgcgtc
     1261 cggagcgtgt tcttcatccc cgaggatctg gcgatcgtgg gcggcgggcc cgaggagcgc
     1321 cggcgcttcc tggacgaggc ggcgtacggg ctctggccga tgcgcgaggg agagcggcgg
     1381 gcctacgacc gggcgctgag gcagcggaac cgcctcctca aggagcacga ggggccgggg
     1441 cccccggccg agctcgaggc ctgggacgcc gagctgatcg cgagcggggc gctggtgacg
     1501 gcggcccggg ccgcggccgc cgagcgcgtc gcccccaggg cgtccgagcg gtacgagggg
     1561 ctctcggggg atcggctgct cgtggcgtac gctccctcgg tagcgggaga ggacctggag
     1621 gaggccttcc gggaggggtt ggctgcccgt cgcggggacg agctgatcag gcgcagcacg
     1681 ctcgtggggc cccatcgaga cgacctcgaa ctggccgtcc gaggtcggac ggcgcggagg
     1741 ttcgcctcgc acggggaggc ctgggcggcg gccctgtgtc tgcggctggg gctgaccggg
     1801 gcgctcgagg ccgaggtggg ggagtccccg gtgctgctgc tggacgaccc cttctccggg
     1861 atcgacccgg agcgccggcg gcgtctcgcg gcctcgctca gcgccggtcc ccagaccatg
     1921 ctcgccctac ccgacgaggc ccagatcccg gatggagccg tcgtgtggga ggtgcgtgat
     1981 ggagcggcca cgccgaggtg acccgccatc cgagcccact ggggtggggg agatcctgcg
     2041 aggcctgctg agcgagccgc cccttcgacg agggctgcgc ctggggcgcc tgggacgggt
     2101 ctggccccgg gtggtggggg agcggctggc cccggtcacg gcgcccgtct ccctggacga
     2161 ggggggcctg gtggtgtcgg cctcgaccca ggcgtgggct gctcaggtgc gcttcatggc
     2221 cgacgagatc ggccggcggg cgggcgaggt tctgggggag gatccgcccc cgccggtgag
     2281 ggtcgtggtc ggcccggagc gacccaagcg agacggctga cgggccaggg gcgacggtcc
     2341 tcgatggtat aatcacatcg acgaaacccg cgcgatgcag cccctttcgg tactgccgcc
     2401 ttcccctgtg cgcggggcca tcacggaggg tacgtggcgc agaagacctc gaagacgacg
     2461 aaacggaccc gcaagagggg ctacacggcc gcagacatca ccgtcctcga gggactcgaa
     2521 gcggttcgca agcgacccgg gatgtacatc gggtcgaccg gcccccgcgg cctccatcat
     2581 ctcgtctacg aggtcgtgga caacgccgtg gacgaggccc tcgccggcca atgcaccaag
     2641 atcaaggtcg tgctgcggaa ggacggcagc gtcctcgtgg aggacaacgg gcgcgggatc
     2701 ccggtcacca agatccccgg gcagaaggac cggcgtcccg ccgtggaggt ggtcctcacg
     2761 accctccacg ccggcgggaa gttcgacggg aagtcctacg cagtctcggg cggcctccac
     2821 ggcgtgggcg tctccgtggt gaacgcgctc tcccgtcgtc tcgaggtcga ggtgatgcgc
     2881 gacggccaca cgtggaccca ggcctacgag cgcggcaagc gcctctctcc gctgaagaag
     2941 ggaaaggcca cgaagaagac cgggaccacg gtggtcttct ggcccgaccc cgacgtcttc
     3001 gaggagctcg acttcaagcg agagctcctc gccgagcgtc tccaggagct ctccttcctc
     3061 accggcggcg tggagatcgc cctcgtcgac gagcgcgaga ggaagccatg ggaggccgtc
     3121 ttcaaggcca aggggggcct ggccgacttc gtcaagcaca tcaaccaggc caaggagccg
     3181 atccacgccc gcgtgatcca cttcgacgcc aagggcaagg atgccgaggt cgaggttgcg
     3241 atgcagtgga acggctccta ctccgagtcg ctccactcct tcgcgaacac gatcaacacc
     3301 cacgagggcg gcacccacga ggagggcttc aagaaggccc tcaccaacgt gatgaaccgg
     3361 tacgcgcgcg ccaagggcct cctcaaggag aaggaggaga acctcatcgg cgaggacatc
     3421 cgcgaggggc tcgccgcgat cctctcggtc aagatcaagg acccgcagtt cgaaggacag
     3481 acgaagacga agctcggcaa caccccgatg cgctccctcg tcgagaccac ggtcaacgag
     3541 cacttcatga actggctcca ggagaacccg ggcccggcga agcgcatcta ccagaagtcc
     3601 gagcaggcgg cgaaggcgcg gctcgcagct cgacaggcgc gggacctgac caggcggaag
     3661 gggctcctcg actcctccct gcccgggaag ctcgccgact gtcagctcac cgaccccgag
     3721 gtgtgcgagc tgtttatcgt ggagggcgac tccgccgccg ggcccgcgat ccaggcccgc
     3781 gacccggcgt accaggcgat cctcccgatc cgcgggaaga tcctcaacgt cgagaaggct
     3841 cgcctgcaca agatccttga gaacaaggag atccaggccc tgatcaccgc gatcggaacc
     3901 ggggtgcacg acgagttcaa cctcgaggac gcccgctacc acaaaatcgt catggccacc
     3961 gacgccgacg tggacggggc gcacatccga accctcgtcc tcaccttcct ctaccgcgag
     4021 atgcccgagc tgatcgacgc cggctacgtg tacatcgcca agccgccgct ctacaagatc
     4081 aagtccggca agcaggacgt ctacatcgag aaggagtccg agctcgagga ggtcctgctg
     4141 cgcgacaagc tcgagaagct cgaggtcacg gaccgcgaag gcgagcagtt cagcctcacg
     4201 cacgcgcgtt ggcagaagta cggccgcctg ctgaagcagt acgagggctg ggcggcggcg
     4261 ctgcgtgccg agtggggcca cgacccgatc accttcctgg aggagtcggc tgtgctcgac
     4321 gagggcgtca ccgacgccga tcaggcgctc gcgatgatca agggcg
//
LOCUS       DASUTW010000130         5278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1595541, whole genome shotgun sequence.
ACCESSION   DASUTW010000130 DASUTW010000000
VERSION     DASUTW010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595541"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VF058_04345"
     CDS             <1..532
                     /locus_tag="VF058_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207571.1"
                     /translation="AYEPNWASLTLTDHDMEEPILNGVDLDVEITYGEESTTMAMEPA
                     FGDTGEYRAVFVPSRPGDYSFRFFGTVRGQRINETFSSGPDTFSPANDPAELSFPAQD
                     PTVAEVAERLEQETTRLNERVTSLEQSIAGQETTEEEDPAAGARTLALIALIVGGVGL
                     VAGLVVGGMALRRAGA"
     gene            537..2492
                     /locus_tag="VF058_04350"
     CDS             537..2492
                     /locus_tag="VF058_04350"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEX6207572.1"
                     /translation="MRRALLLAGMVALGVVVAAPGASGHALLESSAPGDGELLEAAPE
                     EILLSFSEPLDLGLSSVSVVDSQGAEVDAGELERLGSEREVRLALPQDLSDGVYSVSW
                     RVLSRVDGHTTAGAFTFGVGVAASEVTPSGDATQVEYPSPTPLSVGGRWGFYWGLVLL
                     LGAAMGGLFLFPARVRWAAWLLAIGWAVGALGLVAMVVAEASAADIGLGSLLGSDRGT
                     ILLVRAAALVAAGLVGAAALARPGRVAWGALGATTLAAMLVHAYAGHAGAGRNPVLAV
                     GVQWIHIVAVGVWVGGLAWLLVGLVGADSEVRAPAAARFSSIATWAIVFVALTGFLRA
                     WSEVGSLGDLFDTSFGLAAVGKSALFGGMLALGAYNRFRLIPRGRLPTLRRTMSGELA
                     MAVGVFGLTGLMAGLVPPSQLQEEEEVAAAPERVVVEGSDFATTVRMRLTATPGAPGP
                     NAFQARISDYDTGEPVEATAVSLRFSMPDRGDVGTTELELDEVEPGLWRGEGTNLSVA
                     GTWRVGVLIQQPDDAVRLELELETSGAEPEIEVIEGGPGQPDIYVIPLGEGRTVQAYV
                     DPGAAGSNEVHYTFFTPEGGELPMEDIAIRAALEGGEPIELEPRRLTEGHFVASAELE
                     TGAWSFTAEATTEDGTVLTATFEEEIE"
     gene            2489..2929
                     /locus_tag="VF058_04355"
     CDS             2489..2929
                     /locus_tag="VF058_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207573.1"
                     /translation="MRMRLAALAAALVLAACTGNGTEETPTPDTGETTAERPSSPAEL
                     TIVEPEPGAVYPPDDAPVRLELENAEIIEEVSTDLRGDQGHVHLVLDGETVTLLGGLD
                     ENLAELAEGGQLEPGMHILEAEFVAADHGFFLPRVITTVTFQVE"
     gene            2926..3465
                     /locus_tag="VF058_04360"
     CDS             2926..3465
                     /locus_tag="VF058_04360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HEX6207574.1"
                     /translation="MIAEPRTSTRAPRWAAFVAGAGLAALALRAPGVEALVVVAALGV
                     AGLTLPVPARDRPARARAWLAAVAIGALAMALVARAATVPLAFAPLPVGAAVLAAVAE
                     EAFFRRFLYGWLAARGPAVAISLTAAAFALVHLPVYGEASLPLNVAAGALLGWQRWAT
                     GTWSAPAATHALANVLAYA"
     gene            3477..4712
                     /locus_tag="VF058_04365"
     CDS             3477..4712
                     /locus_tag="VF058_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6207575.1"
                     /translation="MHRIVALLLLAAACAPASGSDPLVVGAVYPTAGSQGIGGIEEFR
                     GVRLAVERVNAEGGVEGRLVRLSVQPAESAEGAPLAVRAAAADGAEVILGSYGSTIST
                     KAAKVASDLDLVFWETGAVGEIEDGVRAGERFFRLVSMGGSLGDEGVRFLDEQLLPMM
                     GEDPEALDWGVLYVDDVYGRSVGLGAAATIEEAGLPLAGTFPYDLATLDGERLVRRIG
                     RAGVDVLVVSAYLEDGVELRREMVRQDLPLVASVGTSSSYCMREFGATLGRDAVGLFA
                     SDKPDGEVMGEAELRPEAAEALAWANREYEERWDMPMPAPALSGFAGAWALLHHVLPD
                     AGSANAQEVAEAALAADLPVGSLPNGSGLRFAAEGDPRAGVNLRATSVIWEWVDAQER
                     EVVWPPDVATSPIVPIPIS"
     gene            4709..>5278
                     /locus_tag="VF058_04370"
     CDS             4709..>5278
                     /locus_tag="VF058_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207576.1"
                     /translation="MTRLRWGVAAAGVYLLVAAALLGAGRPVLPLFDGLAPVEYRWVD
                     PPPGFEEGNKQPLDGRSRAPLEEGGAITVITADGQAQISILTEDVVVPEGQRAVVGTL
                     TPVDPGSLGPPPAGLRFDSNAYEAEVTYVTSEDPLGFAEGDPAEDPPSATILLRYAAH
                     ATQLARWDEATSSWTNLEATLLRGTLQIYG"
BASE COUNT          641 a         1840 c         2062 g          735 t
ORIGIN      
        1 ggcctacgag cccaactggg cctcgctgac gctgaccgac cacgacatgg aggagccgat
       61 cctaaacggc gtggacctcg acgtggagat cacctacggg gaggagtcca cgacgatggc
      121 catggagcct gcgttcgggg acaccgggga gtaccgggcc gtcttcgtgc cgtcgcggcc
      181 gggagactac tcgttccggt tcttcggcac ggtccgcggc cagcggatca acgagacgtt
      241 cagctccggc cccgacacct tcagcccggc gaacgacccg gcggagctct cgttccccgc
      301 gcaggacccg accgtggccg aggtggcgga gcgcctggag caggagacga cgaggctgaa
      361 cgagagggtc acctcgctcg agcagtcgat cgccgggcag gagacgaccg aggaggagga
      421 cccggcggcg ggggcgcgca cgctggcgtt gatcgcgctg atcgtcgggg gcgtggggct
      481 cgtggccggc ctcgtcgtcg ggggcatggc gctccggagg gccggggcct agacggatgc
      541 gcagagcgct cctgctcgcc ggcatggtcg ccctgggggt cgtggtcgcg gcccccgggg
      601 cgagcggcca tgcgctgctc gagtcgtcgg ctcccgggga cggggagctg ctcgaggcgg
      661 ccccggagga gatcctcctc tcgttcagcg agccgctgga cctggggctc tcctcggtct
      721 cggtggtgga ctcccagggt gccgaggtcg atgcgggcga gctggagcgc ctgggttccg
      781 agcgggaggt ccgcctggcc ctcccgcagg atctatcgga cggcgtgtac tccgtctcgt
      841 ggcgcgtgct ctcccgcgtg gatgggcaca cgacggcggg ggcgttcacg ttcggcgtgg
      901 gggtggccgc gtcggaggtg acgccgtcgg gagacgcgac gcaggtcgag tacccgagcc
      961 cgacgccgct ctcggtgggc gggcgctggg gcttctactg ggggctcgtc ctcctgctcg
     1021 gcgccgccat ggggggcctc ttcctgttcc cggcccgcgt ccgctgggcg gcctggctgc
     1081 tcgcgatcgg ctgggcggtg ggagcgctcg ggctcgtggc catggtggtc gcggaggcga
     1141 gcgcggccga catcggcctc ggctcgctcc tgggctccga tcgagggacg atcctgctgg
     1201 tccgagccgc ggccctggtc gccgcggggc tcgtgggggc ggcggcgctg gcgaggcccg
     1261 gccgcgtcgc gtggggggcg ctcggcgcca cgaccctggc cgcgatgctc gtgcacgcct
     1321 atgccgggca cgccggcgcg gggaggaacc ccgtcctggc cgtcggcgtg cagtggatcc
     1381 acatcgtggc cgtgggcgtc tgggtggggg gcctggcctg gctgctggta gggctcgtgg
     1441 gggccgactc ggaggtccgg gccccggcgg ccgctcgctt ctcgagcatc gcgacctggg
     1501 ccatcgtgtt cgtcgcgctc acgggcttcc tgcgcgcttg gagcgaggtc gggtcgctcg
     1561 gcgacctctt cgacacgagc ttcgggctcg cggccgtggg aaagagcgcg ctcttcgggg
     1621 gcatgctggc tctgggcgcc tacaaccgct tccgcctcat cccccgaggg cggctgccca
     1681 cactgcggcg gacgatgagc ggcgagctcg ccatggccgt cggcgtcttc gggctcacgg
     1741 ggctgatggc cgggctcgtg cccccctccc agctgcaaga ggaagaggag gtcgcggctg
     1801 cgccggagcg cgtcgtggtg gagggatccg acttcgccac gaccgtgcgg atgcggctca
     1861 ccgccacgcc gggcgcgccg ggccccaacg cgttccaggc tcggatctcc gactacgaca
     1921 ccggcgagcc cgtcgaggcg accgccgtct cgctccggtt ctcgatgccc gaccgcggcg
     1981 acgtggggac caccgagctc gagctggacg aggtggagcc gggcctgtgg cggggcgagg
     2041 gcacgaacct ctcggtcgcc gggacctggc gcgtcggggt cctgatccag cagccggacg
     2101 atgccgtgcg cctggagctg gagctggaga cgagcggtgc cgagcccgag atcgaggtga
     2161 tcgagggcgg gcccggacag cccgacatct acgtgatccc gctcggcgag ggacggaccg
     2221 ttcaggcgta cgtggacccc ggcgcggccg ggtccaacga ggtccactac acgttcttta
     2281 cgcccgaagg cggcgagctg ccgatggagg atatcgccat ccgggccgcg ctcgagggcg
     2341 gagagccgat cgagctcgag ccccggcgcc tgaccgaagg gcacttcgtc gcgtcggcgg
     2401 agctcgagac gggagcctgg tccttcaccg ccgaggccac cacggaagac ggtaccgtgt
     2461 tgaccgcgac cttcgaggag gagatcgagt gaggatgcga ctcgcggcgc tggccgccgc
     2521 cctggtgctc gccgcctgca cgggcaacgg gaccgaggag acgccgacgc cggacacggg
     2581 cgagacgacg gcggagcggc ccagctcgcc cgcggagctc acgatcgtgg agccggagcc
     2641 gggggccgtc tacccgccgg acgacgcgcc cgtgcggctc gagctggaga acgcggagat
     2701 catcgaggag gtctccaccg acctgcgcgg cgaccagggg cacgtccacc tcgtgctgga
     2761 cggcgagacc gtgacgctcc tgggcggcct ggacgagaac ctggcagagc tcgcggaggg
     2821 aggccagctg gagccgggga tgcacatcct ggaggcggag ttcgtcgccg ccgaccacgg
     2881 gttcttcctc ccgcgcgtca tcacaacggt aaccttccag gtcgagtgat cgccgaaccc
     2941 cgcacctcga cccgcgcgcc caggtgggct gcgttcgtgg ccggcgccgg cctcgcggcg
     3001 ctcgccctgc gggcgccggg cgtggaggcg ctcgtcgtgg tcgccgccct gggcgtggcg
     3061 gggctgacgc tccccgtccc ggctcgggac cggcccgctc gagcccgggc ctggctcgca
     3121 gcggtggcga tcggggcgct ggccatggcc ctggtggctc gtgccgccac cgtgcccctg
     3181 gccttcgccc ccctgcccgt cggcgccgcc gtcctggccg cggtggcgga ggaagcgttc
     3241 ttccggaggt tcctgtacgg atggctggcg gcgcgggggc ccgccgtggc catctcgctg
     3301 accgccgcgg cattcgccct cgtccacctg ccggtctacg gggaggcgtc cctgccgctc
     3361 aacgtcgcgg ccggcgcgct cctcggctgg cagcggtggg cgaccgggac ctggagcgcg
     3421 ccggccgcga cccacgcgct cgccaacgtg ctcgcctacg catgaggagg atgaagatgc
     3481 accgaatcgt cgccctgctg ctgctcgcgg cggcgtgcgc ccccgcctcg gggtcggacc
     3541 cgctcgttgt gggtgccgtc taccccacgg ccggcagcca gggcatcggg gggatcgagg
     3601 agttccgcgg ggtccggctc gcggtggagc gggtcaacgc cgaggggggc gtggaaggcc
     3661 gtctcgtccg gctcagcgtc cagccggctg agtcggccga gggagcgccc ctcgcggtgc
     3721 gggccgcggc ggccgacggg gccgaggtga tcctgggcag ctacggcagc acgatctcga
     3781 cgaaggcggc gaaggtggcc tccgacctcg acctcgtctt ctgggagacc ggggccgtgg
     3841 gcgagatcga ggacggggtc cgcgccgggg agcgcttctt ccggctcgtc tccatggggg
     3901 gctcgttggg cgacgagggg gtgcggttcc tcgacgagca gctgctcccc atgatgggcg
     3961 aggacccgga ggcgctcgac tggggggtcc tgtacgtgga cgacgtctac ggccgctcgg
     4021 tggggctcgg ggccgcggcc acgatcgagg aggccgggct gccgcttgcc ggcaccttcc
     4081 cgtacgacct ggccacgctc gacggcgagc gcctcgtccg gaggatcggt cgggccggcg
     4141 tggacgtgct cgtcgtctcc gcctacctcg aggacggcgt ggagctccgc agggagatgg
     4201 tgcgccagga tctgccgctc gtggccagcg tgggcacctc ctcctcgtac tgcatgcggg
     4261 agttcggggc gacgctcggc cgcgacgcgg tagggctctt cgcctccgac aaacccgacg
     4321 gagaggtgat gggggaggcc gaactgcggc cggaggcggc tgaggccctg gcctgggcca
     4381 accgcgagta cgaggagcgg tgggacatgc cgatgccggc gccggccctc tccgggttcg
     4441 ccggggcgtg ggcactcctc caccacgtgc tgccggacgc ggggtcggcc aacgcccagg
     4501 aggtcgccga ggcggcgctg gcggccgacc tgcccgtggg gtccctgccc aacggcagcg
     4561 gcctccggtt cgcggcggag ggcgatccgc gcgccggggt caacctccgc gccacgagcg
     4621 tgatctggga gtgggtcgac gcgcaggagc gcgaggtggt ctggcccccc gacgtggcca
     4681 cgagccccat cgtgcccatc cccatctcat gacgaggctg cgctggggcg tcgccgcggc
     4741 cggcgtctac ctcctggtgg ccgccgcgct cctcggggcg gggcggccgg tactgccgct
     4801 cttcgacggg ctcgcccccg tcgagtaccg ctgggtcgac cccccgcccg gcttcgagga
     4861 gggcaacaag cagcccctcg acggccgttc ccgtgccccg ctcgaggagg gaggagcgat
     4921 cacggtcatc accgccgacg gccaggcgca gatctcgatc ctcaccgagg acgtggtcgt
     4981 ccccgagggt cagcgcgcgg tggtcgggac gctgacgccc gtggatccgg gcagcctcgg
     5041 gccgccgccg gccgggctcc ggttcgactc gaacgcgtac gaggccgagg tgacctacgt
     5101 cacctcggag gacccgctcg gattcgcgga gggcgatccc gccgaggacc cgcccagcgc
     5161 cacgatcctg ctccgctacg cggcccacgc cacccagctg gcgcggtggg acgaagccac
     5221 gagctcgtgg accaacctgg aggccaccct gctgcgagga accctccaga tctacggg
//
LOCUS       DASUTW010000131         5798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1076593, whole genome shotgun sequence.
ACCESSION   DASUTW010000131 DASUTW010000000
VERSION     DASUTW010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076593"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1657
                     /locus_tag="VF058_04375"
     CDS             98..1657
                     /locus_tag="VF058_04375"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6207577.1"
                     /translation="MYRGRIFGKSVLEAQFRAFYGTDLFLGELLAAEDEDRGRRFLFR
                     VIDVTYGSDSSDPRWAERAAGAYMAGDAAGTPYELRDPEQRLYKVARCAPLGYIDGAG
                     EFRKPKSLPTQFSKVTSPTVEDFEFLRGRMGDLRVGLLRSGEDTIDLEVGIRGETLTS
                     HVGVFATTGMGKSNLMKVLSGTILESQGRYAVLLFDPHGEYLDGGGGVRRGLRDHPWA
                     GDRLKVYATREGGGHTALRLALSELTLGDLQTAYEWSHAQEDAIWRLQRLYGEDWIAA
                     VADEALDPQELAEQVGAAASTMQVIQRRARRILELPCISRDPSQPSLSERIVRELQEG
                     YSVLVETSGLSSMEEILAASVITRRLLDTYAGAYLDDREGFDRLPPTLVALEEAQRVL
                     SRAKRSDENVFPRVAREGRKFKVGLCAVSQQPKLIDDELLSQFNTFFVLGLADEKDRN
                     ILRASSKQDVSDLGPEIQTLMPGEAIVTNLDAPFALPARVHLYEEYLKSAPGPAPLPS
                     RTEGASRGFAE"
     gene            1654..2805
                     /locus_tag="VF058_04380"
     CDS             1654..2805
                     /locus_tag="VF058_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011693612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX6207578.1"
                     /translation="MSVRRLAEIPGFNIDRVAAAAGDDPEVLRLENLDTDLSVPAGVI
                     EATRAAVGEDDANSWLPFDGKLEMKEAVADFIERRSGQRYDPARQVVITGSEGDAMLN
                     SLFVTTDPGDEVILTDPTYAGMLNRVRLAGAVPRLVPLVPGSEGWRLDVVALREAVGP
                     STRAVFLMNPSLPTGSVLTEDEWAAVAEVCLERDAWLIYVSMYEGIVFDGLPVFSPAS
                     LPDLRDRLIVAGAVTLEHRMIGWRIGWTVGPKALHEDLSKVQIYNTLCPSGILQVGAR
                     LALGESPGDLAGVVAEYERRRDETMRQLEGFEPVPSSGGWSLLVDTAALGIDPSDASR
                     RLLDEKVAATPMRGWGGEVADRYLRFVYSNESIDRLALLGERARRALGG"
     gene            2848..3984
                     /locus_tag="VF058_04385"
     CDS             2848..3984
                     /locus_tag="VF058_04385"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEX6207579.1"
                     /translation="MARLVALGDAHLGRTHHAHLRDEEGRNQREEDFLRSFDWAVEAT
                     IEREPDGFLWLGDVFDHARPSYRVFTKVLSALKRLEEAGIPGVAISGNHDTPRLRGTG
                     SPYAALEEVFPGVTFAWAMEARSADLAGVRVHAVPQTLTVEDFKAELARASEGLSSDA
                     TNLLLAHVALTSLPLREWRDINELEVEEATFDRRFDLVVLGHYHVFQKASKRTWYAGA
                     TDSFSFADRPKGAGPKGIVVVDTDAGKVEHVPNPGERPLVTFGVDAGGMGPGELVAAA
                     ERHAGEVPRGAIVRLFLNEVDPAAYRQVSQAEFQEAMPAALHVQIEPDYGAAALAVQG
                     GVEMGTLEAEWGGYVERQDLAGLDRDRVRAKGEELLVQAQGDSV"
     gene            3981..>5798
                     /locus_tag="VF058_04390"
     CDS             3981..>5798
                     /locus_tag="VF058_04390"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6207580.1"
                     /translation="MRILELSLRNYRVFEQVDLELPARVIGIFGANGAGKSTLVESIQ
                     FALYGAGRTKKDQIRTHGVLTEAWVRLLFEHGGRQYEVRRVIKGKNHAIEAELLMGDA
                     SLAVGVREVDEEVRKLLRMDQQVFRSSVFAEQKQVDAFSDLTKSKRQEMVLRLLGIRP
                     VDEARTAARKEARDLKKRAEDLAGALPDLTEREAALASAREALAKAAARAEEAAEALG
                     SARAAAEAAREAFEESDRARQEVEQVAGLLAAAEEERERLSARRDELSSRIEASRKDL
                     EELPGLEREALDLAGAREALEAARRWAEVAVELREAEAALASSADVDPAPLLAATEEA
                     RATLKEAEAAAHRAVAGREGAERDLDRAESELAKAGELDASKPCPTCGQPLGDAFQDV
                     VRHREEEARELRAALDAAAAAAEKAEKARTKAETVAEKAVAEAEDARKASEQRSSLEV
                     AADKLRAKAAKAAEPFDGEPPAVDELEARAARAEEVAEHLASLRAQQAGLDQAQADLD
                     SASADLDACLAKITDLTERRDALSFSPERHAELRKRDAEARAAAEQAVRDEREASDAK
                     KDAEREVASLASAIDQVKEAKARAGELQDEARYADRVSLL"
BASE COUNT          838 a         2014 c         2197 g          749 t
ORIGIN      
        1 gagctccggg gggcgctgag ccgggagggc atgagcgagg acgacgtcga ggtcctcttc
       61 caggacttcc acgaggtgct gaacaggagc tgaggcgatg tatcggggca ggatcttcgg
      121 caagagcgtc ctggaggcgc agttccgcgc gttctacggc accgacctgt tcctgggcga
      181 gctgctggcc gccgaggacg aggatcgcgg tcggcgcttc ctcttccggg tgatcgacgt
      241 gacctacggg tccgactctt cggatcctcg ctgggccgag cgcgcggccg gagcgtacat
      301 ggccggggac gccgcgggca cgccctacga gctccgcgat cccgagcagc gcctgtacaa
      361 ggtggcgcgg tgcgcgccgc tcggctacat cgacggcgcg ggcgagttcc gcaagccgaa
      421 gtccctcccc acgcagttct ccaaggtcac ctcccccacc gtggaggact tcgagttcct
      481 ccgcggacgg atgggcgacc tccgcgtggg gctgctgcgg tcgggcgagg acacgatcga
      541 cctcgaggtg gggatccgcg gcgagacgct cacctcgcac gtgggcgtct tcgccacgac
      601 cggcatgggg aagtcgaacc tgatgaaggt gctgtcgggg acgatcctcg agtcgcaggg
      661 ccggtacgcg gtcctgctgt tcgaccccca cggggagtac ctggacgggg gcggcggggt
      721 ccggcggggc ctgcgcgacc acccctgggc cggcgaccgc ctcaaggtct acgccacgcg
      781 cgagggcggc ggccacaccg ccctgcggct ggcgctgtcg gagctcaccc tgggcgacct
      841 ccagaccgcg tacgagtgga gccacgccca ggaggacgcg atctggcgcc tccagcggct
      901 ctacggcgag gactggatcg ccgcggtggc cgacgaggcc ctggatccgc aggagctggc
      961 cgagcaggtg ggcgccgccg ccagcacgat gcaggtgatc cagcggcggg cgcgccggat
     1021 cctcgagctg ccctgcatca gccgcgaccc gtcccagccc tcgctctcgg agcggatcgt
     1081 gcgcgagctc caggaggggt actcggtgct cgtggagacc tcggggctgt cctcgatgga
     1141 ggagatcctc gcggcctcgg tgatcacccg gcggctgctc gacacgtacg cgggcgcgta
     1201 cctcgacgac cgagagggct tcgaccgcct cccccccacc ctcgtggcgc tggaggaggc
     1261 ccagcgggtg ctgtcccggg ccaagcgctc cgacgagaac gtcttccccc gcgtggcgcg
     1321 ggaggggcgg aagttcaagg tggggctctg cgcggtgagc cagcagccga agctgatcga
     1381 cgacgagctg ctcagccagt tcaacacgtt cttcgtgctg gggctcgccg acgagaagga
     1441 ccgcaacatc ctgcgggcct cgtccaagca ggacgtctcc gacctcggtc ccgagatcca
     1501 gaccctgatg ccgggcgagg cgatcgtcac gaacctcgac gcgccgttcg ccctccccgc
     1561 ccgcgtgcac ctgtacgagg agtacctgaa gtcggccccg ggccccgccc cgctcccctc
     1621 gcggaccgag ggcgcctcca gggggttcgc ggaatgagcg tccggcggct ggccgagatc
     1681 ccgggattca acatcgaccg cgtcgcggcc gcggccgggg acgatcccga ggtcctgcgg
     1741 ctggagaacc tcgacaccga cctctccgtg ccggccggcg tgatcgaggc cacgcgggcg
     1801 gccgtgggcg aggacgacgc caacagctgg ctgcccttcg acgggaagct tgagatgaag
     1861 gaggcggtgg ccgacttcat cgagcgtcgc agcgggcaaa ggtacgaccc ggctcgccag
     1921 gtcgtcatca ccggcagcga gggcgacgcc atgctcaact cgctcttcgt caccaccgac
     1981 cccggcgacg aggtgatcct cacggacccc acctacgcgg gcatgctgaa ccgcgtccga
     2041 ctggcggggg ccgtcccccg cctcgtgccg ctcgtgcccg ggagcgaggg ctggcggctg
     2101 gacgtggtgg ccctccggga ggcggtcggc ccctcgacca gggccgtgtt cctgatgaac
     2161 ccctcgctcc ccacggggtc cgtcctcacc gaggacgagt gggcggccgt ggccgaggtc
     2221 tgcctcgagc gcgacgcctg gctcatctac gtctcgatgt acgaggggat cgtcttcgac
     2281 gggctgccgg tgttcagccc cgcgtcgctc cccgacctgc gcgaccggct catcgtcgcc
     2341 ggcgctgtca cgctcgagca ccgcatgatc ggctggcgga tcggatggac ggtcgggccc
     2401 aaggcgctcc acgaggacct gtccaaggtg cagatctaca acacgctctg tcccagcggg
     2461 atcctccagg tgggggctcg cctggcgctc ggcgagtctc ccggcgacct ggccggcgtg
     2521 gtggccgagt acgagcgaag gcgagacgag accatgcggc agctggaggg gttcgagccg
     2581 gtcccctcgt cgggcggctg gtccctcctc gtggacacgg cggccctggg catcgacccg
     2641 agcgacgcct cccgccgact gttggacgag aaagtggcgg cgacccccat gcggggctgg
     2701 ggcggcgagg tggccgatcg ataccttcgg ttcgtctaca gcaacgagtc gatcgaccgg
     2761 ctcgcgctgc tcggcgagcg cgcccggcga gcgctcgggg gctaggatgg taccctcgaa
     2821 tcacatgcgt gtgattcggg agaggtgatg gcgaggctcg tcgcgctcgg agacgcgcac
     2881 ctggggcgga cccaccacgc ccacctccgg gacgaggagg gccgcaacca gcgcgaggag
     2941 gacttcctcc gctccttcga ctgggcggtg gaggccacga tcgagcggga gccggacggg
     3001 ttcctgtggc tgggcgacgt tttcgaccac gcaaggccgt cgtaccgggt gttcacgaag
     3061 gtgctctccg ccctgaagcg gctggaggaa gcggggatcc cgggcgtggc gatctcgggc
     3121 aaccacgaca ccccgaggct ccgcggcacc gggtcgccct acgccgcgct ggaggaggtc
     3181 ttcccgggcg tgaccttcgc gtgggccatg gaggcgcggt cggcggacct ggccggcgtg
     3241 cgcgtccacg ccgtgcccca gacgctgacc gtcgaggact tcaaggcgga gctggcgcgg
     3301 gcctcggagg ggctgtcgtc ggacgccacg aacctcctgc tggcccacgt ggccctcacc
     3361 tcgctccccc tccgcgagtg gcgggacatc aacgagctcg aggtcgagga ggccaccttc
     3421 gatcggcggt tcgacctcgt ggtgctgggc cactaccacg tcttccagaa ggcctccaag
     3481 cgcacgtggt acgcgggggc caccgactcg ttctccttcg ccgaccggcc caagggcgcg
     3541 gggcccaagg ggatcgtcgt cgtggacacc gacgccggca aggtcgagca cgtgcccaac
     3601 ccgggcgagc ggccgctcgt gaccttcggc gtggacgccg gcgggatggg gccgggcgag
     3661 ctggtcgccg cggcggagcg gcacgcgggc gaggtgcccc gcggcgccat cgtccgcctg
     3721 ttcctcaacg aggtggatcc tgccgcgtac cggcaggtct cgcaggcgga gttccaggag
     3781 gcgatgcccg cggccctcca tgtccagatc gagcccgact acggagcggc ggcgctggcg
     3841 gtccagggcg gcgtggagat ggggaccctc gaggccgagt ggggcgggta cgtcgagcgg
     3901 caggacctgg cgggactcga ccgggaccgc gtccgggcca agggcgagga gctgctggtc
     3961 caggcccagg gggactcggt gtgaggatcc tcgagctctc gctgcgcaac taccgggtgt
     4021 tcgagcaggt cgacctggag ctccccgcgc gggtgatcgg catcttcggg gccaacggcg
     4081 cgggcaagag cacgctcgtg gagtcgatcc agttcgcgct gtacggggcg gggcggacga
     4141 agaaggacca gatccggacg cacggggtgc tgaccgaggc gtgggtgcgg ctgctgttcg
     4201 agcacggcgg ccggcagtac gaggtccgcc gggtgatcaa gggcaagaac cacgcgatcg
     4261 aggccgagct gctgatgggc gacgcctcgc tggcggtcgg ggtgcgcgag gtggacgagg
     4321 aggtccggaa gctgctgcgg atggaccagc aggtgttccg gtcgtcggtg ttcgcggagc
     4381 agaagcaggt cgacgcgttc tcggacctca cgaagtcgaa gcggcaggag atggttctgc
     4441 ggctcctcgg gatccggccg gtggacgagg cgcggacggc tgcgcggaag gaggcccggg
     4501 acctgaagaa gcgggccgag gacctggcgg gggccctgcc ggacctgacg gagcgggagg
     4561 cggcgctggc atctgcgagg gaggcgttgg cgaaggcggc ggcgcgggcc gaggaggccg
     4621 ccgaagcgct ggggtcggcc cgcgccgccg ccgaggccgc tcgcgaggcc ttcgaggagt
     4681 ccgaccgggc ccgccaggag gtggagcagg tggcggggct gctcgcggcg gccgaggagg
     4741 agcgagagcg tttgtcggcc cgccgagacg agctctcctc ccggatcgag gcctcccgga
     4801 aggacctcga ggagctcccc gggctggagc gggaggcgct ggacctggcg ggggcccggg
     4861 aggcgctgga ggccgcgcgc cgctgggccg aggtcgccgt ggagcttcgg gaggcggagg
     4921 cggcgctcgc ctcctccgcc gacgtggacc ccgctcccct gctcgccgcg acggaggagg
     4981 ctcgcgccac cctcaaggag gccgaggccg ccgcccaccg cgccgtcgcc gggcgtgagg
     5041 gcgcggaacg cgatctcgac cgggccgagt ccgagctggc caaggccggc gagctcgacg
     5101 cctcgaagcc ctgccccacc tgcggccagc ccctcggcga cgccttccag gacgtcgtcc
     5161 gccaccgcga ggaggaggcc agagagctgc gcgccgccct cgacgcggcc gcggccgccg
     5221 cggaaaaggc ggagaaggcc cggacgaagg cggaaacggt tgccgagaag gctgtggctg
     5281 aggctgagga cgccaggaag gcctccgagc agcgctcctc gctggaggtc gccgccgaca
     5341 agctccgggc caaggccgcc aaagccgcgg agcccttcga cggcgagcca ccggccgtcg
     5401 acgagctcga ggcccgcgcc gcccgcgccg aggaggtcgc cgagcacctc gcctcgctcc
     5461 gcgcccagca ggccggcctc gaccaggccc aggcagacct cgactcggcc tcggccgacc
     5521 tcgacgcctg cctcgccaag atcaccgacc tcaccgagcg ccgcgacgcc ctctcgttct
     5581 cgcccgagcg ccacgccgag ctccgcaagc gcgacgccga ggcccgcgcc gccgcggagc
     5641 aggccgtccg cgacgagcgc gaggcctccg acgccaagaa ggacgccgag cgcgaggtgg
     5701 cctcgctcgc ctccgcgatc gaccaggtca aggaggccaa ggcgcgggcc ggcgagctcc
     5761 aggacgaggc ccgctacgcc gaccgcgtct cgctcctc
//
LOCUS       DASUTW010000132         3806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1466832, whole genome shotgun sequence.
ACCESSION   DASUTW010000132 DASUTW010000000
VERSION     DASUTW010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466832"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VF058_04395"
     CDS             <1..184
                     /locus_tag="VF058_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEX6207581.1"
                     /translation="TEAADARGHRVFPGKLVKKGLSFGEKAIVMALRAPEGDFRDWDE
                     IRGWASEIAEALEGAR"
     gene            315..878
                     /locus_tag="VF058_04400"
     CDS             315..878
                     /locus_tag="VF058_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207582.1"
                     /translation="MAAAFLLLVVPDYRPLLGIAYAPIVLLGAPFHWPPEVGIADVLP
                     WAVVNQLICMAGGVAWAAAAIVHRRRRLGACVRCGRSDEPPRAGDSRWGRWAPYVAVI
                     VPLLYAAMRFAWALGIPLGITEESLREGQETGLRRSGRRLRLRRGRGIGGAGAGDAVA
                     GLGVALAVASLDHHRRRRGRCPRCGRE"
     gene            961..2178
                     /locus_tag="VF058_04405"
     CDS             961..2178
                     /locus_tag="VF058_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207583.1"
                     /translation="MVAAGVLVGTTSAVGAAKPKTRLVSKTSQGQAGDDTSVDARLSN
                     DGRYVVFQSQADNLPGPDDVQGIYVHDRKTKKTRLVSRRSSGQPALGQNRNPDISGNG
                     RFVVFESDASNLPGDGFQVYVHDLKTKKTRLVSKNSAGQPADASGLAGRISDSGRFIT
                     LSSMAGNLPAGGGPLSKLYIHDRKTKKTRLLSRTSSGAPVSAGLHALSASGRFAAFTS
                     SDPALPGPSTFRVYVHDRKTKKTRLVSKRSSGVPFDDFASGVAISGSGRVVAFSLNVD
                     YNEICDRVDIHAHDRKTKKTRLMSRTTAGAAADGCNLRPAISGNGRYVAFQSDAPNLP
                     GASPPIARSYVHDRKRRTTKVLSKTTGGTVVLGATPALSWTGEWATFASNEDALPGPN
                     TGVFQVYVRGPLR"
     gene            complement(2175..3278)
                     /locus_tag="VF058_04410"
     CDS             complement(2175..3278)
                     /locus_tag="VF058_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX6207584.1"
                     /translation="MPVLTKRTLNRALLGRQLLLERASLSVPRAVEHLVGLQAQEPPN
                     PYVALWSRLESFRHQDLADLLVRRRAVRLTLMRGTIHLVTARDALAMWPVMRPQLERS
                     FHGHLWGRSLTDLDLEELLAAGRELVEEQPRTAAQIREAIAERWPDHDETSLVSAVRY
                     LLPMVQVPPRGVWGASGLPTLTTAERWLGRPLGKGARPDRMILRYLAAFGPASIADLR
                     TWSGLTGLREVVERLRPRLRTFRSEEGRELFDVEDGLLPEPDRDAPPRFLPTYDNVTL
                     SHDDRRRIVEDEDRRRMQRGMLRGNVGSVLVDGFVRGMWKVSRSRDHPTLEIVPLGRL
                     SKVESTAVGTEGERLLAFVAGDAPDQDVRIARG"
     gene            3363..>3806
                     /locus_tag="VF058_04415"
     CDS             3363..>3806
                     /locus_tag="VF058_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX6207585.1"
                     /translation="MDDRWYKNAIIYEIDLRSFADSDGDGIGDLRGLIDRLDYLAALG
                     ITCIWLLPFSPSPWRDGGYDVTDHYGVHPELGTPGDFVELVHRAAARGIRVIIDLVLN
                     HTSDEHPWFRAARDPDSPYRDHYVWAETPPDDAESGVVFPGVQDST"
BASE COUNT          550 a         1350 c         1339 g          567 t
ORIGIN      
        1 cacggaagcc gccgacgccc gcggccaccg cgtcttcccc gggaagctcg tcaagaaggg
       61 cctcagcttc ggcgagaagg cgatcgtgat ggccctccgc gcgccggagg gtgacttccg
      121 ggactgggat gagatccggg ggtgggcgtc ggagatcgcc gaggcgctgg agggcgcgcg
      181 gtaggcctcg ctccgtacgc ggcggccgtc tggtcgttcg cctacggggc ctgggtctcc
      241 tttgggccct gggcgctccg gggttcccct acggccgcgg gatcgccgcc ggcgtcctcc
      301 tcggcttcgc ctgggtggcg gccgccttcc tgcttctcgt cgtccccgac taccggccgc
      361 tcctggggat cgcctacgcc ccgatcgtcc tcctcggcgc tcccttccac tggcctcccg
      421 aggtcgggat cgccgacgtt ctgccttggg cggtcgtgaa ccagctcatc tgcatggcgg
      481 gcggcgtggc atgggcggcc gcggcgatcg tgcatcggcg cagacgcttg ggagcgtgcg
      541 tccgctgcgg ccgctccgac gagcctccgc gcgccggcga ctctcgctgg ggccggtggg
      601 cgccctacgt ggccgtgatc gttccgctgc tctacgcggc gatgcgcttc gcctgggcgc
      661 tggggatccc cctggggatc accgaggagt ccctgcgaga ggggcaggaa accggtctcc
      721 ggcgctctgg gcggcgcctt cgccttcgcc gaggacgtgg gatagggggc gctggggccg
      781 gagatgctgt ggccggcctg ggggtcgccc tggccgtggc ttcgctcgac caccaccggc
      841 gacgacgggg ccgttgtcct cggtgcggga gggaatgacc caccccaagg atcgcggaag
      901 aacccgtggt acggtgccgc ccccgaggag agggagggtc atgcgaaggt cggcatcctc
      961 gtggtcgccg ccggcgtcct ggtcggcacg acgtcggcgg tcggcgcggc caagcccaag
     1021 acgcggctgg tcagcaagac gtcccagggc caggccggcg acgacacgag cgtggacgcg
     1081 cggctctcga acgacgggcg ctacgtcgtg ttccagtcgc aggcggacaa cctgcccggc
     1141 cccgacgacg tccagggcat ctacgtccac gaccgcaaga cgaaaaagac gcgcctcgtg
     1201 agcaggagat cgagcggcca gcccgccctc ggtcagaacc ggaaccccga catctcgggg
     1261 aacgggcgct tcgttgtctt cgagtccgac gcctccaacc tgccggggga cggcttccag
     1321 gtgtacgtcc acgatctgaa gacgaagaag accaggttgg tcagcaagaa ctcagccggc
     1381 cagcccgccg acgcctccgg tctcgccggc cggatctccg actccgggcg gttcatcacc
     1441 ctctcctcca tggcggggaa ccttcccgcg ggcggaggtc ctctgtcgaa gctctacatc
     1501 cacgaccgga agacgaagaa gacgcggctg ttgagcagga cctcgagcgg cgctcccgtg
     1561 agcgcaggtc tccatgcgct ctccgcgagc ggacggttcg cggcgttcac ctcgagcgac
     1621 ccggccctcc ccggacccag cacgttcagg gtctacgtcc acgatcggaa gacgaagaag
     1681 acccgtctcg tgagcaagag gagctccggg gttcccttcg acgacttcgc gagcggggtc
     1741 gccatctccg ggagcgggag ggtggtcgcc ttctccctca acgtggacta caatgaaatc
     1801 tgcgacagag tggacatcca cgcccacgat cggaagacga agaagacgcg gctgatgagc
     1861 aggaccaccg cgggggccgc ggccgacggc tgcaacctgc gcccggccat ctcggggaac
     1921 ggcaggtacg tggccttcca gtccgacgcc ccgaacctcc ccggagcttc gccgccgatc
     1981 gcccgctcct acgttcacga tcggaagcgg cggacgacga aggtgctgag caagacgacg
     2041 ggggggacgg tcgtgctggg cgccactccc gcgctctcgt ggacggggga gtgggcgacc
     2101 ttcgcgtcga acgaggacgc gctgcccggc ccgaacaccg gcgtcttcca ggtctacgtc
     2161 cgcgggccgc tgcgctagcc gcgggcgatc cggacgtctt ggtcgggggc gtccccggcg
     2221 acgaaggcga ggaggcgctc gccttccgtc cccacggcag tggactccac cttgctgagc
     2281 ctcccgagcg ggacgatctc gagcgtcgga tgatcccgtg atcgcgagac cttccacatc
     2341 cctcgaacga atccgtccac gagcacgctg cccacgttcc cccggagcat ccctcgctgc
     2401 atccggcgcc gatcctcgtc ctccacgatg cggcggcgat cgtcgtggga cagcgtcacg
     2461 ttgtcgtagg tgggcaggaa ccgtgggggc gcgtcgcggt ccggttcggg gaggagcccg
     2521 tcctccacgt cgaacagctc tcggccctcc tcgctccgga aggtgcggag ccgggggcgg
     2581 agacgctcga cgacctcccg cagccccgtc aggcccgacc aggtccgcaa gtcggcgatc
     2641 gaggccggcc cgaacgcggc gagatatctg aggatcatgc ggtccggtcg cgcgcccttc
     2701 cccaggggac gtcccagcca tcgttccgcc gtggtcaacg tggggaggcc gctcgcgccc
     2761 cacacgcctc ggggcggcac ctgcaccatc ggcaggaggt agcgaacggc cgagacgagc
     2821 gaggtctcgt cgtggtcggg ccagcgctcc gcgatggcct cccggatctg ggcggcggtt
     2881 ctcggctgct cttcgacgag ctctcgcccc gcagccagga gctcctcgag atcgagatcc
     2941 gtgaggctcc gtccccagag gtgtccgtgg aagctgcgct cgagctgggg cctcatcacc
     3001 ggccacatcg cgagcgcgtc ccgggccgtg acgaggtgga tcgtgccccg catcagcgtc
     3061 aggcgcaccg cccggcgtcg gaccaggagg tcggcgagat cctggtgacg gaagctctcc
     3121 agccggctcc atagcgcgac gtaggggttg ggcggctcct gagcctggag gccgacgagg
     3181 tgctccactg cccggggcac ggacagggag gctcgctcga gcaggagctg ccgtcctagc
     3241 agggctcggt tcagcgttcg cttcgtcagc acgggcatcc gggaagcatg ccgcgccggc
     3301 gcgccgcgcg cacgccgcgt gccgtcggtt gcggcgctcg ctccgcgggt aactccccgg
     3361 gcatggacga ccgctggtac aagaacgcga tcatctacga gatcgacctc cgctcgttcg
     3421 cggattccga cggcgacggg atcggcgacc tccgtgggct gatcgatcgg ctcgactacc
     3481 tcgccgctct cgggatcacc tgcatctggc tgctgccctt ctcgccctcc ccctggaggg
     3541 acggcggcta cgacgtgacc gaccactacg gggtccatcc cgagctcggg acgcccggcg
     3601 acttcgtgga gctcgtgcac cgtgccgccg cccggggcat ccgggtgatc atcgacctcg
     3661 tcctaaacca cacatccgac gagcacccct ggttccgggc cgcgcgggac ccggactcgc
     3721 cctacaggga ccactacgtc tgggcggaga ctcccccgga cgacgccgag agcggcgtgg
     3781 tgttcccggg cgtccaggac tcgacg
//
LOCUS       DASUTW010000133        10279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_689670, whole genome shotgun sequence.
ACCESSION   DASUTW010000133 DASUTW010000000
VERSION     DASUTW010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10279
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689670"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..817
                     /locus_tag="VF058_04420"
     CDS             86..817
                     /locus_tag="VF058_04420"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6207586.1"
                     /translation="MTLVSDVMTTDVVSVEEDTPFKEIARLMHEHRISGVPVVDEGGH
                     LQGIVSEADVLAMEEDKVEPKHRRSFLEWFIDPRRLSEIEARAEDARAKDIMTSAVVT
                     VHSDTPIKRAITRLLDAGVKRLPVVDEDGKLVGIVSRTDLMSPLLRPDEEIEREVKED
                     VILRSMWIDPTMMVVEVEEGVVRLEGRVESRSVKEILLDMVRRVDGVVGVDADLLEYE
                     VDDRDVEPPPSQAGLRWGENWARRE"
     gene            complement(819..1601)
                     /locus_tag="VF058_04425"
     CDS             complement(819..1601)
                     /locus_tag="VF058_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005520452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6207587.1"
                     /translation="MELGLAGKKAVVTGGSKGLGRAIAEELASEGMDVAICSRTEDEV
                     RAAGEELRRRGVTVHAEAADVTNPAQVERFIAAAAETLGGIDVLVNNAGRAHPGTFAT
                     LTDEDWREDLDVKLFSMIRCSREALPHLRARGGGRIININAVYAKYPDPTFFATSTNR
                     AASVAFTKTLSMELANDGILVNSVNIGFVVTPQWENIHRKRAPELSREEFFARMAAAE
                     VPLGRFGTPDEVSGIVAFLASARASYITGASIDVAGGMGRYL"
     gene            complement(1601..1996)
                     /locus_tag="VF058_04430"
     CDS             complement(1601..1996)
                     /locus_tag="VF058_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6207588.1"
                     /translation="MTDADRYFTLDEDLQRAGEGHFLDLDGIEPAEFVPGLVFRPVLG
                     DRVMVNWVHFDPNTEAPKHAHQEEQITFVVEGEFEFDLDGDVRMMKPGMVAVIPAGVP
                     HGARTHDSSCLEIDVFVPPRKALLELTQD"
     gene            complement(1993..3186)
                     /locus_tag="VF058_04435"
     CDS             complement(1993..3186)
                     /locus_tag="VF058_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX6207589.1"
                     /translation="MRATTDWGFARRTRVDVGEGLASILALSGATDLISFAGGFPDPE
                     TFPAAELAGLFERVAQDPAALQYAPTPGLPGPRGFMADRLEAREGLRPADEELLITSG
                     GIEALELLGKSLIDPGDLVLVEGPTYLGAIMAFRSFEADVVAVPMDDDGLDVGRLEET
                     VLERGRPKLLYTIPDHQNPAGVSLAEERRGALVEVARRHGFLVVEDVAYRELGFEDAR
                     PPSLWSLGPDVVVQVGTFSKTFFPGVRLGWAAGPVEVVTQMVNAKQNTDQCAGALGQR
                     LLEEYGRAGGLEAQAERARELYGRRCRLMLEALEAHLPEGIRWTRPRGGFFTWLTYLE
                     GVDTAALAKEAMERKVAFVPGKPFFPDGTGANTLRLSFSRVRDEEIDEGIRRLAEVSA
                     EGGTG"
     gene            3233..3547
                     /locus_tag="VF058_04440"
     CDS             3233..3547
                     /locus_tag="VF058_04440"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEX6207590.1"
                     /translation="MRAQHVVLFKFPRPLSEDEEREMFDQVRAWPRTIPGFTGLRLGR
                     DVSGRNEGYDYLLLTEFESEEAHQAYYAQPAHRSFSEWVFSRDCEVIRVDYPLDERNL
                     IV"
     gene            complement(3544..4314)
                     /locus_tag="VF058_04445"
     CDS             complement(3544..4314)
                     /locus_tag="VF058_04445"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX6207591.1"
                     /translation="MQRLAETIVLTEEDFKTLGEADFGVPGLIAHEWIGPFVEVQAVG
                     PLIMVHDGIFDPRKGITHHPHRLNERLFYILEGTVDHDDALNDITGHMGTGDLGRLTE
                     GRRGMFHQEWNHTDGRARAFILVYGTDPVPPTASFQALRDADAPRYEASPGVISKEIV
                     GPKADLPLHGDIRLWTDDRMERGSDCALDLDEGEGGLVFPIEGAVVVDGSELRPEAMA
                     ILPPSGSSRSVTVSSPEGARVLRVVFGPGHGLALRSAG"
     gene            4358..5422
                     /locus_tag="VF058_04450"
     CDS             4358..5422
                     /locus_tag="VF058_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX6207592.1"
                     /translation="MSVPTTMRAARLHRVRSEDQPELISVDEVPIPEPGPGEVLVRIA
                     ACGICASDLHVADGTTPGGPTPLILGHEAAGVVVTVGGGVTSWSVGDRVALLMARACG
                     DCPACEAGRESLCARLAIPGIDGDGSQAEYAVARDRDLLPVPEGILLEQAAILTDAVA
                     TPFHAMRRSGLEPGMSCAIFGLGGLGMHALLLARALGASWVAGVDLDPANLERALAWG
                     ADEAIDAGDGKPAREVRGRSGGGVDRAFEFVGSPASQSQTVKSLRPGGRATIVGLTPA
                     PLDLLPAALLVSQELEVVGSFGSSRADLRELLEMVERGDLDLARSVTHRWPLDEFPEG
                     LRALETRESHPIRSVVTHEA"
     gene            complement(5488..5925)
                     /locus_tag="VF058_04455"
     CDS             complement(5488..5925)
                     /locus_tag="VF058_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207593.1"
                     /translation="MMRNRVPALALLVALVAAGCGGGEETVEVTLQEFAILPAEDTVS
                     AGSVTFDATNEGPDDPHELVVIQTDLAPEALPTTDEGGVDEEGEGIEVIGEIEEFPVG
                     ETESATFDLDAGDYVLVCNIVEEEDGELESHYALGMRIGFVVE"
     gene            complement(5941..6660)
                     /locus_tag="VF058_04460"
     CDS             complement(5941..6660)
                     /locus_tag="VF058_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009882543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEX6207594.1"
                     /translation="MDARAAAQELERRLGAVARAERAEHERRYLKSSLRHLGVSVPAI
                     RRTALGFLEEHELDRSELLALARALWKEPVHERRMAAVEILAERADLLGPDDLPLVEE
                     LLRDSRTWALVDPLAIQVAGSLVDRWPELGSTLDRWAGDEDFWVRRSAMLALLSGLRA
                     GAGDLDRFLSYADAMLEEREFFIRKAIGWVLRETGKRRPGVVARWLEPRAGRASGVTL
                     REAVKHLSEGQQERILRAAGH"
     gene            complement(6665..7123)
                     /locus_tag="VF058_04465"
     CDS             complement(6665..7123)
                     /locus_tag="VF058_04465"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEX6207595.1"
                     /translation="MPTLYEWLKFAHVLLAIIAVGFNASYAIWLARAARHPEREPFAL
                     RTIKLLDDRFANPAYGLLLVTGLLMIWNADIPISTFWISTSIGIYVAVLLLGLGVYTP
                     TLRKQIRLLEEDGPDASDYRRLSNRGTVVGILLGIMVVVIVFLMVTKPML"
     gene            7268..8272
                     /locus_tag="VF058_04470"
     CDS             7268..8272
                     /locus_tag="VF058_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6207596.1"
                     /translation="MPDIGIVVGGHMSLIADLAKAAEDGGYESVWVAETSHTPFVQAA
                     VAAQATSRVTIGTAIALAFPRSPTVVASTARDLQELSGGRFVLGLGSQVKRVNVQRFG
                     VPFEHPAPKMAEYAEVVRGVLATFDGAPIDHRGRFYEITMPPFPGASSVPRIPLYLAG
                     VNEKMIETAGRVYDGLLGHPMTSPAYVADVVRPAVERGAAAAGRDPSEVDLTTSVIMQ
                     VHDDVETARRQAAYQVAFYATTRTYKPVLALHGFEELSAPLREAHAARDLERMADLAM
                     PMVDTYAICGPPEACREQLAAYDGLVDRIVLGGAWVGPDRSAIVENYRRLLSDFTPRG
                     "
     gene            8269..9159
                     /locus_tag="VF058_04475"
     CDS             8269..9159
                     /locus_tag="VF058_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207597.1"
                     /translation="MSEPPDEVRRLAEERAGARAARDFDRADALRERLTALGWEVRDE
                     PGGYELSPAGPAPRVPPEAVPSRLEEPPTHDVTVHWLAERWWDDVLRGVASFRRHEGD
                     RRVEHLVVEADPAPPATWPRDLRVLPLDRDPGFGSARAAGLRQTMGRIAIVADASVEA
                     VGDPYAPIERALADETIGVCGPVGAVTRDLRSFEESSGPDVDAIQGYLMALRRDLLAR
                     VSFDPGYRYYRWADVDLSFQVKALGLRAVRVDVPMRRHEHRGWAALDPEERDRRSRRN
                     HARFLRRFGGRSDLLVSPPA"
     gene            complement(9143..10012)
                     /locus_tag="VF058_04480"
     CDS             complement(9143..10012)
                     /locus_tag="VF058_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX6207598.1"
                     /translation="MSRLSGTLAASVTPLEDGGARPDEDAVGPVVDFLHEGGLDGILA
                     LGTTGEGLMLSGHERRRVAERFVEASARRLRVAVHCGAQTTSETAALAAHAAEAGADA
                     VAVISPPYYPFDPAGLLRHFREAAAAAAPLPFYVYEFADRSGYAVPVEVLSGLRGVAD
                     NFLGLKVSDAPLERFAPYLLEGLDVLVGPEALILEGMGRGAVGAVSGLASAFPELVAE
                     LVRSRDPALGERVAALRARLQRLPVPAVFKAALRLRGVPVREDVRPPLRTLTDAERAE
                     VAAAVEGAATPAG"
     gene            complement(10009..>10279)
                     /locus_tag="VF058_04485"
     CDS             complement(10009..>10279)
                     /locus_tag="VF058_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207599.1"
                     /translation="LTQARERARAILEPKKDLMLRLAKVLMARETIEGSELKAWVDGD
                     EPVPEPDEEPRAEEATRRLEPPEAAADGEAGGDGARATEEIHRPT"
BASE COUNT         1437 a         3686 c         3705 g         1451 t
ORIGIN      
        1 cggaccaagg aagaggccga cgcggccgag cactgagcgc acgcattgct ctaggcttgt
       61 cccgtccttc gatctggggg tgacgatgac gcttgtgtcc gacgtgatga cgaccgacgt
      121 ggtgtccgtg gaggaggaca cgcccttcaa ggagatcgcc cgactcatgc atgagcaccg
      181 gatcagcggc gtgccggtcg tcgacgaggg gggacacctg caggggatcg tgagcgaggc
      241 cgacgtgctc gccatggagg aggacaaggt cgagcccaag caccggcgga gcttcctgga
      301 gtggttcatc gacccgaggc gcctctcgga gatcgaggcc cgagcggagg acgcccgagc
      361 gaaggacatc atgaccagcg ccgtggtgac cgtgcactcc gacacgccga tcaagcgagc
      421 catcacgcga ctgctcgatg cgggcgtcaa gcgcctcccg gtggtagatg aggacgggaa
      481 gctcgtgggc atcgtcagcc gaacagacct catgtcgccg ctgctccgcc ccgacgaaga
      541 gatcgagcgc gaggtgaagg aggacgtgat cctgcggtcg atgtggatcg acccgacgat
      601 gatggtcgtc gaggtggagg agggggtcgt ccgactcgag ggacgcgtcg agagccgcag
      661 cgtgaaggag atcctgctcg atatggtgcg cagggtcgac ggggtcgtcg gcgtggacgc
      721 cgacctgctc gagtacgagg tggatgatcg ggacgtcgag cccccgccct cccaggccgg
      781 cctccggtgg ggcgagaact gggcgcgtcg ggagtgacct agaggtagcg gcccatgccg
      841 ccggcgacgt cgatgctcgc cccggtgatg tagctcgcgc gggcactggc caggaaggcc
      901 acgatccccg agacctcgtc gggcgtcccg aacctcccca gcggcacctc ggccgccgcc
      961 atccgggcga agaactcctc ccgggagagc tccggcgcgc gcttgcgatg gatgttctcc
     1021 cactgcggcg tcacgacgaa cccgatgttc acgctgttga cgaggatccc gtcattggcc
     1081 agctccatcg acagggtctt ggtgaacgcg acgctggccg cgcggttggt ggaggtggcg
     1141 aagaaggtcg ggtcggggta cttggcgtag accgcgttga tgttgatgat tcggccgccg
     1201 cctcgggctc gcaggtgggg gagggcctcg cgtgagcagc ggatcatcga gaagagcttg
     1261 acgtcgaggt cctcccgcca gtcctcgtcg gtcagggtgg cgaaggtgcc ggggtgcgcg
     1321 cggccggcgt tgttgaccag gacgtcgatc ccgccgagcg tctccgccgc cgcggcgatg
     1381 aaccgctcga cctgcgcggg gttcgtgacg tcggcggcct cggcgtggac cgtgacgccg
     1441 cggcggcgga gctcctcgcc ggccgcgcgc acctcgtcct cggtccgcga gcagatcgcg
     1501 acgtccatcc cctcggaggc gagctcctcg gcgatggcgc ggcccagccc cttcgagccg
     1561 ccggtgacga cggccttctt tcccgcgagt ccgagctcca tcagtcctgg gtcagctcga
     1621 ggagggcctt ccggggcggg acgaagacgt cgatctcgag acaggagctg tcgtgggtcc
     1681 gtgcgccatg ggggacgccg gcggggatca ccgccaccat ccccggcttc atcatcctga
     1741 cgtcgccgtc gagatcgaac tcgaactcac cctccacgac gaaggtgatc tgctcctcct
     1801 ggtgcgcgtg cttcggggcc tcggtgttcg ggtcgaagtg gacccagttg accatcaccc
     1861 ggtcgcccag cacgggccgg aagacgagcc cgggtacgaa ctccgccggc tcgatcccat
     1921 ccaggtcgag gaagtgcccc tcgcccgcgc gctgcaggtc ctcgtccagc gtgaagtagc
     1981 ggtcggcgtc cgtcatcccg tccctccctc ggccgaaacc tcggccagcc tgcggatccc
     2041 ctcgtcgatc tcctcgtccc gaacccggct gaaggagagc cggagcgtgt tggcgccggt
     2101 cccgtcgggg aagaagggtt tccccggcac gaaggcaacc ttccgctcca tcgcctcctt
     2161 cgccagcgcg gcggtgtcca cgccctcgag gtacgtgagc caggtgaaga agccgccccg
     2221 ggggcgcgtc cagcggatgc cctcggggag gtgagcctcc agggcctcca gcatgaggcg
     2281 gcagcgcctc ccgtagagct cgcgggcgcg ctcggcctgc gcctccaggc cgccggcgcg
     2341 cccgtactcc tcgaggaggc gctggccgag cgcgcccgca cactgatcgg tgttctgctt
     2401 cgcgttcacc atctgcgtca cgacctcgac cggcccggcc gcccagccca gccgaactcc
     2461 cgggaagaac gtcttggaga acgtcccgac ctggaccacg acgtccggcc ccaggctcca
     2521 gaggctcggg ggacgggcgt cctcgaaccc cagctcacgg taggcgacgt cctccacgac
     2581 gaggaacccg tggcggcgcg ccacctccac cagcgccccc cgtcgctcct cggccaggct
     2641 gacccccgcc gggttctggt ggtcggggat cgtgtacagg agcttcggcc ggccccgctc
     2701 gaggaccgtc tcctcgagcc gccccacgtc gagcccgtcg tcgtccatcg gaaccgccac
     2761 cacgtccgcc tcgaagctgc ggaaggccat gatcgcgccg aggtaggtgg gcccctccac
     2821 cagcacgagg tcgcccgggt cgatcaggct ctttcccagc agctcgaggg cctcgatccc
     2881 gccgctcgtg atcaatagct cctcgtcggc cgggcgcaag ccctcccggg cctccagacg
     2941 gtcggccatg aagccgcgtg gtcccggcag cccgggcgtc ggtgcgtact ggagcgcggc
     3001 ggggtcctgg gccacccgct cgaacagccc ggcgagctca gcggcgggga aggtctccgg
     3061 gtcggggaag ccccccgcga acgagataag gtcggtggcg cccgacagcg cgaggatcga
     3121 cgccaggccc tcccccacgt cgacgcgcgt cctgcgcgcg aagccccagt cggtggtcgc
     3181 tctcatgctc tcctccaggc cgcacgctac catgcgcccg ggaggtggag ccgtgcgagc
     3241 gcagcacgtg gtgctgttca agttcccgcg gcccttgtcg gaggacgagg agcgggagat
     3301 gttcgaccag gtccgtgcct ggccccggac gatcccgggg ttcacgggcc tgcggctggg
     3361 cagggacgtc agcggccgca acgagggcta cgactacctg ctgctcacgg agttcgagag
     3421 cgaggaggcc caccaggcct actacgcgca gcccgcgcac aggtcgttca gcgagtgggt
     3481 gttctcgcgc gactgcgagg tgatccgggt ggactatcct ctcgacgaac ggaacctgat
     3541 cgtctagccc gccgagcgca gggccagccc gtggcccggc ccgaacacga cgcgcaggac
     3601 gcgggctccc tccggcgagg agacggtcac gctgcgggag gacccgctcg gggggaggat
     3661 cgccatcgcc tcgggccgga gctccgaccc gtccacgacc acggccccct cgatcgggaa
     3721 cactaggccg ccttcgccct cgtcgagatc cagcgcgcaa tctgaccccc gctccatccg
     3781 gtcgtcggtc cacaatcgga tgtcgccgtg gagcggcagg tcggccttcg gccccacgat
     3841 ctccttggag atgacgcccg gcgacgcctc gtagcggggg gcgtcggcgt cgcgaagggc
     3901 ctggaaggag gcggtgggcg gcacggggtc ggtcccgtag acgaggatga aggctcgggc
     3961 ccggccgtcg gtgtggttcc actcctggtg gaacatcccc cggcgtccct cggtgagccg
     4021 ccccaggtcg ccggtgccca tgtggccggt gatgtcgttg agcgcgtcgt cgtggtccac
     4081 cgtgccctcg aggatgtaga acagccgctc gttcagccgg tgggggtggt gcgtgatccc
     4141 cttcctcgga tcgaagatcc cgtcgtggac catgatcagg gggcccacgg cctgcacctc
     4201 gacgaagggg ccgatccact cgtgggcgat caggcccggg acgccgaagt cggcctcgcc
     4261 cagggtcttg aagtcctcct cggtcaggac gatggtctcg gcgagtcgct gcatgcctcc
     4321 actgtacccg tcacgggagg cgggtaggct ggcggcgatg agcgtaccga ccacgatgcg
     4381 cgcggcccgt ttgcaccgtg tccggtccga ggaccagccc gagctgatct cggtggacga
     4441 ggtccccatc cccgagcccg gaccggggga ggtcctggtg cggatcgcgg cctgcggcat
     4501 ctgcgccagc gacctccacg ttgcggatgg gaccacgccg ggcggcccga cgcccctcat
     4561 cctcggccac gaggccgccg gtgtggtggt gacggtcggt gggggcgtga cgtcgtggtc
     4621 ggtcggggac cgggtggcgc tgctgatggc gagggcctgc ggcgactgcc cggcctgcga
     4681 ggccgggcgc gagtcgctct gcgcgcggct ggcgatcccg gggatcgatg gcgacggctc
     4741 gcaggccgag tacgcggtgg cgcgcgatcg ggacctcctg ccggtgccgg aggggatcct
     4801 cctcgagcag gcggccatcc tgaccgatgc cgtggccacc ccgttccacg ccatgcggcg
     4861 ctcggggctc gagccgggca tgtcgtgcgc gatcttcggt ctggggggtc tggggatgca
     4921 cgcgctcctc ctggcgcggg cgctgggggc ctcgtgggtg gcgggcgtcg acctcgaccc
     4981 ggccaacctg gagcgagcgc tggcgtgggg agcggacgag gcgatcgacg ccggcgacgg
     5041 caagccggcc cgcgaggtcc ggggccgatc ggggggcggg gtcgatcgag cgttcgagtt
     5101 cgtggggagc cccgcctcgc agagccagac ggtgaagtcc ctccgcccgg ggggacgggc
     5161 cacgatcgtg gggctcacgc cggccccgct cgacctcctg cccgcggccc tgctcgtctc
     5221 ccaggagctg gaggtcgtgg gctcgttcgg atcgtcgcga gccgacctcc gggagctgct
     5281 cgagatggtc gagcggggcg acctggacct ggcgcgctcg gtcacccacc gctggccgct
     5341 cgacgagttc cccgaggggc tccgcgcgct cgagacccgc gaaagtcacc cgatccggtc
     5401 cgtcgtcacc cacgaggcct gaggcgggct cttccgttca cccctcccga cagacgttcc
     5461 cttccggtgc agggccttcg gaacccccta ctccacgacg aagccgatcc tcatcccgag
     5521 ggcgtagtgg gactcgagct cgccgtcctc ctcctcgacg atgttgcaga cgaggacgta
     5581 atcgccggcg tcgaggtcga aggtggcgga ctccgtctcg cccacgggga actcctcgat
     5641 ctccccgatg acctcgatcc cctcgccctc ctcgtcgacc ccgccctcat cggtggtggg
     5701 gagcgcctcg ggggcgaggt cggtctggat gaccacgagc tcgtgcgggt cgtcggggcc
     5761 ctcgttcgtg gcatcgaacg tcacggatcc cgccgaaacc gtgtcctcgg cgggtaggat
     5821 ggcgaactcc tggagggtga cctccaccgt ctcttcgccg cctccgcacc ccgccgccac
     5881 cagagcgacg agcagggcga gagctggcac ccggttcctc atcatggcgc ctcctcctcc
     5941 tcagtgtccg gcggctcgca ggatgcgctc ctgctgcccc tcggacagat gcttgaccgc
     6001 ctcgcgcagg gtcacccccg atgctcggcc cgctcgcggc tcgagccagc gggccacgac
     6061 gcccgggcga cgcttgcccg tctcgcgcag cacccacccg atcgccttcc ggatgaagaa
     6121 ctcgcgctcc tcgagcatgg cgtcagcgta gctcaggaag cgatcaaggt cccccgcgcc
     6181 ggcccgcagg ccggacagca gcgcgagcat ggccgaccgg cggacccaga agtcctcgtc
     6241 gccggcccac cggtccaggg tcgagccgag ctcgggccac cgatcgacga gcgagccggc
     6301 cacctggatc gccagcggat ccacgagggc ccacgtacgg gagtcacgga gcaactcctc
     6361 gacaagggga agatcgtcgg gccccagcag gtcggcccgc tcggcgagga tctcgaccgc
     6421 cgccatcctc cgctcgtgca ccggctcctt ccacagggcc cgggcaagcg ccaggagctc
     6481 ggaccgatcg agctcgtgtt cctcgaggaa gccaagggcc gtccggcgga tcgccgggac
     6541 gctgacgccg aggtggcgca gagagctctt caggtagcgt cgttcgtgct cggctcgctc
     6601 ggcccgggcc accgctccga ggcgccgctc gagctcctga gccgccgccc gcgcgtccac
     6661 gcgctcagag catcggcttc gtgaccatga ggaagacgat caccacgacc atgatcccca
     6721 ggaggatccc gaccaccgtc ccccggttcg acagacgtcg gtagtccgag gcatccggac
     6781 cgtcctcctc cagcaggcgg atctgcttgc ggagggtcgg ggtgtacacg cccaggccga
     6841 ggaggaggac cgcgacgtag atcccgatcg aggtcgagat ccagaaggtc gagatcggga
     6901 tgtcggcgtt ccagatcatc aggagcccgg tgaccagcag cagcccgtag gcagggttgg
     6961 cgaagcgatc gtcgagcagc ttgatcgttc ggagcgcgaa cggctcccgc tcggggtgcc
     7021 tcgccgcgcg ggccagccag atcgcgtacg aggcgttgaa gccgacggcg atgatggcca
     7081 gcagcacgtg cgcgaacttc agccactcgt agagcgtcgg cacggtcgca ggctaccggg
     7141 gcgtgcgagc cgggtgcaag gcttcgacac ctcgaggagc acgaccgtga gctcggggag
     7201 gagctcccgc cactcgacga gctgagctga ggggcggctc ccccctgcgg cgggtagact
     7261 gccgcccgtg cccgacatcg gcatcgtcgt cggggggcac atgtccctca tcgcggatct
     7321 ggcgaaggcg gccgaggacg gcggctacga gagcgtctgg gtggcggaga cctcgcacac
     7381 gccgttcgtc caggcggcgg tggccgcgca agcgacctcg agggtcacga tcggcaccgc
     7441 gatcgcgctg gccttccccc ggagccccac cgtcgtggcc tccacggccc gcgaccttca
     7501 ggagctgtcg gggggccgct tcgtgctcgg cctcggctcg caagtgaagc gggtgaacgt
     7561 ccagcgattc ggggtgccgt tcgagcatcc cgcgcccaag atggccgagt acgcggaggt
     7621 cgtgcgcggg gtgctcgcca ccttcgacgg ggcgccgatc gatcaccgcg gccgcttcta
     7681 cgagatcacg atgcccccct tccccggcgc ctcgtccgtg cctcgcatcc ccctatacct
     7741 ggcgggcgtg aacgagaaga tgatcgagac cgccggccgc gtctacgacg gcctcctggg
     7801 gcacccgatg acgtcccccg cgtacgtggc cgacgtggtc aggcccgctg tggagcgggg
     7861 tgccgccgcc gccgggcgcg atccgagcga ggtcgacctc accacgagcg tgatcatgca
     7921 ggtccacgac gacgtcgaga cggcccgccg tcaggccgcg taccaggtcg ccttctacgc
     7981 cacgacccgg acctacaagc cggtgctggc cctgcacggg ttcgaggagc tgtcggcgcc
     8041 cctccgggag gcccacgccg ctcgcgacct cgagcggatg gccgacctcg ccatgccgat
     8101 ggtggacacg tacgcgatct gcgggccgcc cgaggcctgc cgcgagcagc tcgccgccta
     8161 cgacggcctc gtggaccgga tcgtcctggg cggggcctgg gtgggcccgg atcgctcggc
     8221 gatcgtggag aactaccgcc ggctcctctc ggacttcacc ccacgcgggt gagcgagcct
     8281 ccggacgagg tccgccggct ggcggaggag cgggccgggg cacgcgcggc gcgggacttc
     8341 gaccgtgccg acgcgctccg cgagcgcctg acggcgctgg gctgggaggt ccgcgacgag
     8401 ccggggggct acgagctgtc gccggcgggc cccgcgccgc gcgtgccccc cgaggccgtc
     8461 ccgtcgcgct tggaggagcc ccccacccac gacgtgaccg tccactggct ggccgagcgc
     8521 tggtgggacg acgtgctccg gggcgtggcc tccttccgcc gccacgaggg cgatcggcgg
     8581 gtcgagcacc tcgtcgtgga ggccgatccc gccccgccgg ccacctggcc ccgggacctc
     8641 cgggtcctgc cgctcgaccg cgatcccggg ttcgggtcgg cgagggcggc cggcctccgg
     8701 cagacgatgg gacggatcgc gatcgtggcc gacgcctcgg tggaggcggt gggcgatccc
     8761 tacgccccga tcgaacgggc gctcgccgac gagacgatcg gggtctgcgg gcccgtgggc
     8821 gcggtcacgc gggacctccg ctcgttcgag gagtcctccg gcccggacgt cgacgcgatc
     8881 caaggctacc tgatggccct gcggcgcgac ctcctggccc gcgtgtcctt cgatcccggc
     8941 taccgctact accggtgggc ggacgtcgat ctaagcttcc aggtgaaggc actggggctc
     9001 cgagccgtcc gcgtcgacgt tccgatgcgg cggcacgagc accgagggtg ggccgccctc
     9061 gatcccgagg agcgtgatcg gcgctccagg cgcaaccacg ccaggttcct ccggcggttc
     9121 ggcgggcgga gcgacctcct cgtcagcccg ccggcgtagc cgcgccttcc acggcggcgg
     9181 caacctcggc ccgctccgcg tcggtcaggg tccgcagcgg ggggcggacg tcctcccgca
     9241 cggggacgcc ccggagcctc agcgcggcct tgaacactgc agggaccggg aggcgctgca
     9301 gccgggcccg gagggccgcg acccgctccc cgagggcggg gtcccgggag cggaccagct
     9361 cggccacgag ctcggggaag gcgctggcca gcccggagac cgccccaacc gctccgcgcc
     9421 ccatcccctc gaggatcagc gcctcgggcc cgaccagcac gtcgagcccc tcgagcaggt
     9481 agggagcgaa ccgctccagc ggggcgtcgg acaccttcag gcccaggaag ttgtcggcca
     9541 cgccccggag cccgctcagg acctcgaccg gcaccgcgta cccgctgcgg tcggcgaact
     9601 cgtagacgta gaaggggagg ggcgccgcgg cggcggccgc ctcgcggaag tgacggagga
     9661 ggcccgcggg gtcgaagggg tagtaggggg gcgagatcac cgccacggcg tcggcgccgg
     9721 cctcggccgc gtgcgccgcc agcgcagccg tctcgctcgt ggtctgcgcg ccgcagtgga
     9781 cggcgacccg cagccgccgt gcggaggcct ccacgaaccg ctcggcgacg cgtcgccgct
     9841 cgtggccgga gagcatgagg ccctcgccag tcgtgccgag cgcgaggatg ccgtccagcc
     9901 ctccctcgtg gaggaagtcg acgacgggtc cgaccgcgtc ctcgtccggc cgagccccgc
     9961 cgtcctccag tggggtcacc gaggcggcga gggtgccgga gagcctgctc acgtgggccg
    10021 gtggatctcc tcggtcgccc gcgccccgtc gcccccggcc tcgccgtcgg ccgcggcctc
    10081 cggaggctcg agccgccgcg tggcctcctc ggcccggggc tcctcgtcgg gctcggggac
    10141 cggctcgtca ccgtcgaccc aggccttcag ctccgagccc tcgatcgtct cccgggccat
    10201 gaggaccttg gccagccgca gcatgaggtc cttcttcggc tcgaggatcg cgcgggcccg
    10261 ctcccgggcc tgggtgagg
//
LOCUS       DASUTW010000134         4415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_689986, whole genome shotgun sequence.
ACCESSION   DASUTW010000134 DASUTW010000000
VERSION     DASUTW010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689986"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VF058_04490"
     CDS             complement(<1..540)
                     /locus_tag="VF058_04490"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX6207600.1"
                     /translation="MPVPLARVIRSGIEESIHLGSVAVADAQGRVVASAGEPDRLTYL
                     RSAWKPLQAAASLARIGDLSDREIAVMAASHNAEEVHLEAVRSLLARAGLDEAALGCP
                     PSRPIDPEPRSEPPAPILHDCSGKHAGMLLACVRAGWETDSYLDPGHPLQREIVELTG
                     AAAGAPVSALGVDGCGAPAA"
     gene            complement(557..1273)
                     /locus_tag="VF058_04495"
     CDS             complement(557..1273)
                     /locus_tag="VF058_04495"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX6207601.1"
                     /translation="MDEQQVRDQLVVFAREIREIYRKERERSAELAHALDQLNDSYLS
                     TMKTLAFLVEAKDVGTRMHLDRTSQYGMALARLVAPELAERPDLEWGFLLHDIGKIGI
                     SERILNKAGPLTTSEWSVMKTHPLIGAQVVAPMAFLGRSSDVIRCHHERWDGRGYPRG
                     LKGEEIPLSARIFSVVDAFDAMTSDRPYRRALSVRDAVAEIARCSGAQFDPDVVEAFL
                     ILMSGMAEPVEARPLLAPTG"
     gene            complement(1452..1991)
                     /locus_tag="VF058_04500"
     CDS             complement(1452..1991)
                     /locus_tag="VF058_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aerial mycelium formation protein"
                     /protein_id="HEX6207602.1"
                     /translation="MSDAPMLGGKRRIDRILADDFVRDLESLETDDLRARRDLSKAER
                     EYLSFLRRLLQGRRDILRDELRRRETGGEAGPLVERITAVLGEGPRGRSRGEAVSVPL
                     PEEEVTLARRRIERLLSDASLSDLEGLSDDDLRETIERVEREERTVSEARGRVMGVHD
                     RLQGEMKRRLRAQLKGLRA"
     gene            complement(2055..3104)
                     /locus_tag="VF058_04505"
     CDS             complement(2055..3104)
                     /locus_tag="VF058_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX6207603.1"
                     /translation="MRGRTSTHIWAVALAGALLASLLTGPVGAQSTKEQLEDARARLA
                     KVESEAQAATAEYEAARGRLIQTEDEIQSTRQRLEAVAKRVNRLQTRLGERASEAYKM
                     GASGMLDLLFVSESLSEFSDRMVFLDRIAQEDADLVLRADVLAERLARYEADLDALMK
                     RQAEATKILREKRELIYEKLEEAQALQAKLEKKLAAEEAAARAAAQAASRSAPGSTDV
                     VVGGALQACPVPGSSFVDSWGAPRSGGRSHQGVDMRAPYGTPVYAATAGSMTQSSSSL
                     GGNQAYVRAPNGDVTFYAHLQGYEGGPRGVSAGELIGYVGDTGNATGTPHLHFEYHPG
                     GGAAVNPYPYVAAVC"
     gene            3271..3963
                     /locus_tag="VF058_04510"
     CDS             3271..3963
                     /locus_tag="VF058_04510"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEX6207604.1"
                     /translation="MGGISPARWLFGLFLVALGVLFLLDTTDLIEATGFWSLAWPTLL
                     VFVGVSILMRERWRSIVGLIVLLLGLGFFAQNAGWLEEGWIGRYWPIVLILAGIGVIV
                     EAMGFVRQSPRTAETGEASDEGSYTAILGGRKSRVTSAAWRGGRATAILGGVELDLRD
                     AVPVAGGAVLDVTAFMGGVDVTVPRGWRVEIDGMPLLGGFDDKTDGGEGPEAPLIRIT
                     GTAIMGGVDVKH"
     gene            3969..4364
                     /locus_tag="VF058_04515"
     CDS             3969..4364
                     /locus_tag="VF058_04515"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX6207605.1"
                     /translation="MMDGGTERPPLRRSRTDRIVGGVAGGLARYFGIDPILVRLGFIL
                     LTIAGGSGVLIYLIAWVIIPEEEAEGADRERAGPAMDAATTRLVLGGALIAIGGILLA
                     SRFIPYFSRVFWPLVLIVAGAAVIMGARR"
BASE COUNT          590 a         1578 c         1592 g          655 t
ORIGIN      
        1 ggcggccggc gcgccgcacc cgtccacgcc cagcgcggag accggcgcgc cggccgccgc
       61 cccggtcagc tccacgatct cccgctggag ggggtgcccg gggtcgaggt acgagtccgt
      121 ctcccatccg gcgcgaacgc acgccagaag catccccgcg tgcttgcccg agcagtcgtg
      181 caggatcggc gccggcggct ccgaacgggg ctcggggtcg atgggccggc tcggcgggca
      241 tcccagggcg gcctcgtcca gcccggccct cgccaacagg gaccggaccg cctccaggtg
      301 cacctcctcg gcgttgtggg aggcggccat gaccgcgatc tcccgatcgg agaggtcacc
      361 gatcctggcc aggctcgccg cggcctggag cggcttccat gccgagcgga ggtaggtgag
      421 gcggtcgggc tcgcccgcgg aggcgaccac ccgaccctgg gcatcggcca ccgcgaccga
      481 cccgagatgg atcgactctt cgatgcccga acggatcacc cgcgcgagag ggaccggcac
      541 ggggacagcg tagcggtcag ccggtggggg cgagcagggg acgggcctcg accggctcgg
      601 ccatgccgct catgaggatc aggaacgcct cgaccacgtc ggggtcgaac tgcgcccccg
      661 agcagcgggc gatctccgcc accgcatctc ggacggagag ggcgcgccgg tacgggcgat
      721 cgctcgtcat cgcgtcgaac gcgtccacca ccgagaagat ccgggccgag agggggatct
      781 cctcgccctt caggcctcga gggtatcccc ggccgtccca ccgctcgtgg tgacagcgga
      841 tcacgtcgct ggatcgcccc aggaaggcca tcggggcgac cacctgcgcg ccgatcagcg
      901 ggtgggtctt catcacggac cactccgagg tcgtgagggg gcccgccttg ttgaggatcc
      961 gctcggagat cccgatcttg ccgatgtcgt ggaggaggaa cccccactcc aggtcggggc
     1021 gctccgccag ctcgggcgcc acgagcctgg ccagcgccat gccgtactgg ctggtgcggt
     1081 cgaggtgcat gcgggttccg acgtccttcg cctccaccag gaaggccagc gtcttcatcg
     1141 tggagaggta cgagtcgttc agctgatcga gggcgtgcgc cagctcggcg gagcgctctc
     1201 gctccttccg gtagatctcc ctgatctccc gggcgaacac caccagctga tccctgacct
     1261 gctgctcgtc cacggaaccc cccggtctcc cgcccgcgcc cggcggcgcg tcggggccgc
     1321 agtataagcc cgcgatcccc cgtctcctcg gagccccgga ggatcgcctg atcgaatggt
     1381 cccatcaccc aggaaggcgg cccatcccgc ggtacgccga tttgcgcgca tcccccgaac
     1441 gggggacggt gtcaggcccg gagccccttg agctgagcgc ggagccgccg cttcatctcc
     1501 ccctgcaggc ggtcgtggac gcccatgacc ctccccctgg cctcggacac ggtgcgctcc
     1561 tcccgctcca cccgctcgat cgtctcgcgc agatcgtcgt cggagaggcc ctcgaggtcc
     1621 gacaggctgg cgtccgagag gagccgctcg atgcgccgcc tggcgagcgt gacctcctcc
     1681 tcggggaggg ggacgctcac ggcctcgcct cgggagcgcc cccgggggcc ctctcccagc
     1741 acggcagtga tccgctcgac cagcgggccg gcctcgcccc ccgtctcacg ccggcggagc
     1801 tcgtcgcgga ggatgtcgcg ccgcccctgc aggagtcgtc ggaggaacga gaggtattcg
     1861 cgctccgcct tgctcaggtc acgccgggcg cggaggtcgt ccgtctccag cgactcgagg
     1921 tcccggacga agtcgtccgc caggatccga tcgatgcggc gcttgccgcc cagcatcggg
     1981 gcatcgctca cgagggctcc ttccaggggg ccacggctcg cattatcgct cggatcgggg
     2041 cgcggggaca gacctcagca gacggcggcc acgtacgggt aggggttgac ggcggccccg
     2101 ccgcccgggt ggtactcgaa gtggaggtgg ggcgtgccgg tcgcgttccc cgtgtcgccg
     2161 acgtagccga tcagctcgcc cgcggagacg cccctcggcc cgccctcgta gccctgcagg
     2221 tgggcgtaga aggtcacgtc gccgttcggc gcccgcacgt acgcctggtt cccgcccagg
     2281 ctgctcgagg actgggtcat gctcccggcc gtcgccgcat acacgggcgt gccgtagggg
     2341 gccctcatgt ccacgccctg gtggctgcgg ccgcccgagc ggggcgcgcc ccagctgtcc
     2401 acgaaggacg agcccgggac cggacaggcc tggagggcgc cgcccacgac cacgtcggtc
     2461 gagcccggcg ccgagcggct ggcggcctgc gccgccgctc gggcggccgc ctcctcggcg
     2521 gccagcttct tctccagctt ggcctggagc gcctgggcct cctcgagctt ctcgtagatc
     2581 agctcccgct tctcgcgaag gatcttcgtg gcctcggcct gccgcttcat cagggcgtcg
     2641 agatcggcct cgtacctcgc gagccgctcg gcgaggacgt cggcccgcag gacgaggtcg
     2701 gcgtcctcct gggcgatgcg gtcgaggaac accatccgat cggagaactc tgagagcgac
     2761 tcggagacga agaggaggtc gagcatcccc gaggcgccca tcttgtaggc ctcgctggcg
     2821 cgctcgccga ggcgcgtctg caggcgattc acccgcttgg cgacggcctc caggcgctgg
     2881 cgcgtggact ggatctcgtc ctcggtctgg atcagccggc cccgagccgc ctcgtactcc
     2941 gccgtcgcgg cctgggcctc gctctcgacc ttcgccaggc gggccctcgc gtcctcgagc
     3001 tgctccttcg tgctctgcgc gccgaccggc ccggtcagga gggaggcgag gagggcgccg
     3061 gcgagcgcca cggcccagat atgggtgctg gtccttcctc gcaaacgact cctcccccct
     3121 ggtcgccgcg tcggccaagg ccacgcgata ccctaccagc cgacccgagg ggcttcaaac
     3181 agcgagtcag gcacgggccg cgaccccggc tcgatgaact ggaacccgct cccgacctgc
     3241 gattatgatg cacgcggagg aggggacccc atgggaggca tctcaccggc tcgctggctc
     3301 ttcggcctgt tcctggtcgc cctgggcgtc ctctttctcc tcgacacgac cgacctgatc
     3361 gaggcgacgg gcttctggag cctggcctgg cccacgctcc tcgtcttcgt gggggtctcg
     3421 atcctcatgc gcgagcggtg gcggagcatc gtgggactca tcgtgctcct cctgggactg
     3481 ggattcttcg ctcagaacgc gggctggctc gaggaagggt ggatcggccg ctactggccg
     3541 atcgtgctga tcctcgccgg gatcggggtc atcgtggagg ccatggggtt cgtgagacag
     3601 agcccgcgga cggcggagac gggcgaggcg agcgacgagg ggtcctacac cgccatcctg
     3661 ggcgggcgga agtcgcgggt cacgagcgcg gcatggcgcg gggggagggc gacggccatc
     3721 ctgggcggcg tggagctcga cctgcgcgat gccgtccccg tggccggagg cgcggtgctg
     3781 gacgtcaccg cgttcatggg gggcgtggat gtgaccgtcc cccgtggatg gcgggtggag
     3841 atcgacggca tgccgctgct gggaggcttc gacgacaaga cggacggagg cgagggtccg
     3901 gaggcgcccc tgatcaggat caccggcacc gccatcatgg gcggggtcga cgtcaagcac
     3961 tgagggggat gatggacgga ggaaccgagc ggccgccgct ccgcaggagc cgcaccgacc
     4021 ggatcgtcgg gggcgttgcc ggcggcctcg cacgctactt cgggatcgac ccgatcctgg
     4081 tcaggctcgg cttcatcctg ctcacgatcg cgggcggctc gggggtcctg atctacctca
     4141 tcgcctgggt gatcatcccc gaggaggagg cggagggggc cgaccgggag cgcgcggggc
     4201 cggccatgga cgcggccacc acccggctcg tgctgggcgg ggcgctgatc gcgatagggg
     4261 ggatcctgct ggcttcgcgc ttcatcccct acttctcgag ggtgttctgg ccgctcgtcc
     4321 tcatcgtggc cggcgcggcc gtgatcatgg gagcgaggcg atgaccgagg agcgcgggat
     4381 ccccgtcggc cggctcgtct tcggagcgat cctgc
//
LOCUS       DASUTW010000135         4689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1597107, whole genome shotgun sequence.
ACCESSION   DASUTW010000135 DASUTW010000000
VERSION     DASUTW010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597107"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VF058_04520"
     CDS             complement(<1..279)
                     /locus_tag="VF058_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010596602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6207606.1"
                     /translation="MRINLTSVYVDDQEKALRFYTEVLGFQKKTDMPLGEGNRWLTVV
                     SPEDPDGPELLLEPSEHPAATPFKEALVSDGIPFTSFAVKDVRAEAERL"
     gene            complement(276..557)
                     /locus_tag="VF058_04525"
     CDS             complement(276..557)
                     /locus_tag="VF058_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6207607.1"
                     /translation="MSDVYRAIADPSRRAILDELVDRDGQTLFEICARLAMKHGLTSS
                     RQAVSQHLAVLEEAGLVRTRREGRYKFHFLDTRPLGEVIERWPIERRGP"
     gene            complement(612..1700)
                     /locus_tag="VF058_04530"
     CDS             complement(612..1700)
                     /locus_tag="VF058_04530"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX6207608.1"
                     /translation="MIIDEPVRGRYAYLEHPWLFGLPPHEQMRPFIERTVPLPPIHHL
                     TGLAPLESSPASATWSMPASPWWRSAAGLFPGGAIAMVVDAALGMAIYGSLPPGTALA
                     TSELTLNFVRPATPASERLLATAGLIHVGSRQGLSDARVTDDHGRLIAHASSRCVRRS
                     LPFDPPEPPATLPSFEEPDQDPPDPYLRPAEGEVIPQEEWDALPGLELVSSWMSGKRE
                     TPPVCRLSGWRLIEVDRGEASWAMPASEWYCTAFGTFYGGAVAHLLDGAINTALTSTL
                     PAGSSFGTLDLKVNFLRPVTPDGRDLVARAKVVHRGRTVAVTRAEMDDADGKRIAMAT
                     GSAMILPGVPWNAAKPQETLDEAPDEES"
     gene            complement(1772..2671)
                     /locus_tag="VF058_04535"
     CDS             complement(1772..2671)
                     /locus_tag="VF058_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207609.1"
                     /translation="MLPALARGRRVATIVAITAALMMLGQTAQTVPVSLDGSNSEVWL
                     FGAGHTRWDVDNNAFACEADLPGYAPVDDGEIGLGTGRTDAFDGGLLLMVGPPTGQPD
                     DATTFHDSDSIVDKVGEQVKAGPELLEGLRVTRLDRVLPKTPTMRTLVALRNTTGSAK
                     ARDIYLDSDLGSDSNTEIRGSSNGSATLLATTRWAVTSDSATDPGDPVVTHVLWGKKA
                     PRRTTEVLESPSGANTCMVIRMRIRVPRKSVRYLLFFAEMTPTNIAGVNGAGKFNKRK
                     LNKALLAGIGKKVRGRILNWKLT"
     gene            complement(2881..>4689)
                     /locus_tag="VF058_04540"
     CDS             complement(2881..>4689)
                     /locus_tag="VF058_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX6207610.1"
                     /translation="GTAVFLYGGLVFLQAAVRELRARLPGMMVLIALAITVAFVFSWV
                     VQLRLIDAEALWWELATLVTIMLLGHWIEMRSITQAQGALQELAKLLPDTATRVTDAG
                     EETVPVNDLRDGDIVLVRPGESIPADGVVRKGESDLNEAMITGESRPVKKEEGDEVIA
                     GTINGEGSLRVEVTGTGEETKLSGIMRLVADAQTSKSRAQHLADRAAQILTGVAIAAG
                     VITLIVWQLLGAAIDFSIVRTVTVLVIACPHALGLAVPLVVAISTTLGAQNGLLVRDR
                     RGLEEARNVDTVIFDKTGTLTLGEFRVVRMDVANETSEEDALRVAAAVESESEHPIAR
                     GIVRTAEDRRLDVPSAEGFRALTGRGVAASVDETEYHLGGPALLQAEDARVPEHLEKA
                     ASAAADRGQAAIYLLGDGEALAVFAVADGIREESREAIRALHDRGIEVAMLTGDAQAV
                     ADAVAEELGIDRVFAEVLPEDKASKVKELQGQGKRVAMVGDGVNDAPALATAEVGIAI
                     GAGTDVAVEAGDIVLVRSDPRDIPRIVTLSRGTYRKMLQNLWWAAGYNIFAIPLAAGA
                     LAAWGILLTPALGAVFMSASTVIVAINAQFLRRARL"
BASE COUNT          675 a         1673 c         1595 g          746 t
ORIGIN      
        1 cagccgctcc gcctcggccc gaacgtcctt cacggcgaag gacgtgaacg ggatgccgtc
       61 cgagacgagc gcctccttga acggcgtcgc cgcaggatgt tcgctcggct cgagcagcag
      121 ctcggggccg tcgggatcct ctggcgagac gacggtgagc caccggttac cctcgccgag
      181 cggcatgtcg gtcttcttct ggaacccgag cacctccgtg tagaagcgca gggccttctc
      241 ctggtcgtcc acgtagacgg acgtcaggtt gatcctcatg gccccctcct ctcgatcggc
      301 caccgctcga tcacctcgcc gagcgggcgt gtgtcgagga agtggaactt gtaccggccc
      361 tcccgcctgg tcctcacgag ccccgcctcc tccagcacgg cgaggtgctg ggagacggcc
      421 tggcgagacg aggtgagccc gtgcttcatg gccagacggg cgcagatctc gaacagcgtc
      481 tgcccgtccc tgtcgaccag ctcgtcgagg atggctctcc tcgaggggtc ggcgatcgcc
      541 ctgtacacat ccgacacacg tgcggcctac gataggcaag tagtgacttg cctgtcaagc
      601 ggaggctcgg gtcagctctc ctcgtccggc gcctcgtcga gggtctcctg gggcttcgcg
      661 gcgttccagg ggacgcccgg caggatcatg gcgctgccgg tggccatcgc gatccgcttc
      721 ccatcggcgt cgtccatctc cgccctggtc acggccacgg tccggcctcg atgcacgacc
      781 ttggcccggg cgaccaggtc ccttccatcg ggggtgaccg gccggaggaa gttcaccttg
      841 aggtcgagcg ttccgaagga cgagcccgcg ggcagcgtcg aggtcagcgc cgtgttgatc
      901 gctccgtcga gcaggtgggc gaccgctccc ccgtagaagg tcccgaaggc cgtgcagtac
      961 cactcgctgg cgggcatggc ccagctcgcc tcgccgcggt cgacctcgat cagccgccac
     1021 ccggagagcc ggcagacggg cggggtctcg cgcttcccgc tcatccacga actgacgagc
     1081 tcgaggcccg ggagcgcatc ccactcctcc tgggggatga cctccccctc ggccggacgg
     1141 aggtaggggt cgggcgggtc ctggtccggc tcttcgaagg aaggaagggt ggccggcggc
     1201 tcgggagggt cgaagggcag cgagcggcgg acgcatcgcg agctggcgtg ggcgatcagt
     1261 cggccgtgat cgtcggtgac ccgagcgtca gagagcccct gacgcgaccc gacgtggatc
     1321 aaccccgccg tggccagcag ccgctcgctg gccggcgtgg cggggcgcac gaagttcagc
     1381 gtcagctcgg acgtggccag ggcggtcccc gggggaaggc ttccgtagat cgccatcccc
     1441 agcgcggcgt ccacgaccat ggcgatcgcc ccgcccggga acaggccggc cgccgaccgc
     1501 caccaggggc tcgcgggcat cgaccaggtg gcggaggcgg gcgaggactc gagcggcgcg
     1561 aggcccgtca gatgatggat cggggggagc ggcaccgtgc gctcgatgaa ggggcgcatc
     1621 tgctcgtggg gcgggaggcc gaagagccag ggatgctcga ggtacgcgta gcgtcctcgc
     1681 accggctcgt cgatgatcac cccgagagga tagacgcgcg ccgatcgggt cgccgccgct
     1741 ccaggctcca gccggccccc ctcccctccc tttacgtcag cttccagttg aggatcctgc
     1801 ccctcacctt cttgccgatg ccggccagga gcgccttgtt cagcttcctc ttgttgaact
     1861 tccccgcgcc gttcaccccg gcgatgttcg tgggggtcat ctcggcgaag aacagcaggt
     1921 agcggacgct cttccggggc acccgaatcc gcatccggat caccatgcag gtgttggccc
     1981 cggaggggct ctcgagaacc tccgtcgtcc tgcgtggggc cttcttcccc cacagcacgt
     2041 gggtgaccac cgggtcgccc gggtcggtcg cgctgtcgct ggtgaccgcc caccgagtgg
     2101 tggcgaggag ggtcgccgac ccgttgctcg agccgcggat ctccgtgttc gagtccgagc
     2161 cgaggtccga gtccaggtag atatctcggg ccttcgccga gccggtggtg ttgcgcaggg
     2221 cgacgagggt tcgcatcgtg ggcgtcttcg ggaggacgcg atccagtcga gtcacccgaa
     2281 ggccctccag gagctccgga ccggccttca cctgctcgcc caccttgtcg acgatgctgt
     2341 cgctgtcgtg gaacgtcgtt gcgtcgtccg gttgtcccgt gggcggaccg accatgagca
     2401 ggaggccccc gtcgaaagcg tccgtccgac ccgtgccgag cccgatctcc ccatcgtcaa
     2461 ccggtgcgta tccggggagg tcggcctcgc atgcaaaggc gttgttgtcc acgtcccatc
     2521 tggtgtggcc ggctccgaag agccacacct ccgagttact tccgtccagg gacacgggga
     2581 ccgtctgggc cgtctggccc aacatcatga gcgcggccgt gatcgccacg atcgtggcga
     2641 ccctgcggcc gcgggcgagc gcgggaagca tagtctctcc ttccttgctc cggttccgga
     2701 cagcatgaac gacggacgcc cccgtgtgaa gagggagcat cggcacgtcg ggtaacggct
     2761 cacgatctcg ggcggtgggt gcccggtacc ggccggccct gccagcaccc gggcggggcc
     2821 ggtgggggtg cctggttggc ttcttgtctc ctggctaggt gaaaggtctc tgggaagcag
     2881 tcacaggcgg gcccttctca ggaactgtgc gttgatcgcc acgatcacgg tgcttgcgga
     2941 catgaacacg gcacccaggg caggtgtcag caagatcccc cacgcggcaa gcgccccggc
     3001 cgccaggggg atggcgaaga tgttgtagcc ggccgcccac cacaggttct ggagcatctt
     3061 gcggtacgtc ccccgggaga gagtgacgat ccgggggatg tcgcgagggt ccgagcgcac
     3121 cagcacgatg tccccggcct ccaccgccac gtccgtcccc gccccgatgg cgatgccgac
     3181 ttcggcggtg gcgagggccg gcgcatcgtt caccccgtcc cccaccatgg ccacccgctt
     3241 gccctgcccc tggagctcct tcaccttgga cgccttgtcc tcgggaagca cctccgcgaa
     3301 cacccgatcg atcccgagct cctctgcgac cgcgtcggcc accgcctgcg cgtcgccggt
     3361 gagcatcgca acctcgatgc cccggtcgtg gagggcgcgg atcgcctcgc ggctctcctc
     3421 ccggatcccg tcggccacgg cgaagaccgc cagggcctcg ccgtcgccga gcaggtagat
     3481 cgcggcctgc ccgcggtccg ccgcggccga ggccgccttc tcgaggtgct cgggcacccg
     3541 agcgtcctcc gcctggagga gcgccgggcc gccgaggtgg tactcggtct cgtcgacgga
     3601 ggcggcgacg cctctcccgg tcagcgcccg gaacccctcc gccgagggca cgtccagcct
     3661 ccggtcctcc gccgtcctca cgatcccccg ggcgatggga tgttcggact cggactccac
     3721 cgcggcggcg acccggagag catcctcctc cgacgtctcg ttggccacgt ccatccgcac
     3781 cacgcggaat tccccaaggg tgagcgtccc cgttttgtcg aagatcacgg tgtccacgtt
     3841 ccgggcttcc tccagccccc gccgatcccg caccaggagg ccgttctgcg cccccagggt
     3901 ggtggagatg gccacaacaa gggggacggc cagccccagg gcgtgtgggc aggcgatgac
     3961 cagcacggtc accgtcctga cgatggagaa gtcgatcgcc gcgccgagga gctgccacac
     4021 gatgagggtg atcacccccg ccgcgatggc caccccggtg aggatctggg ccgcccggtc
     4081 ggccaggtgc tgggcccgag acttggacgt ctgggcgtct gccacgaggc gcatgatccc
     4141 ggagagcttc gtctcttccc cggtccccgt gacctccacc cggagggagc cctcgccgtt
     4201 gatggttccc gcgatgacct cgtcgccttc ctccttcttc accggcctgg actcgccggt
     4261 gatcatggcc tcgttgaggt cgctctcccc cttgcgaacc acgccatccg ccgggatgct
     4321 ctcgccgggg cggaccagca cgatgtcccc gtccctgagg tcgttgacgg gaaccgtctc
     4381 ctctcccgcg tccgtgaccc gggtggcggt gtcgggaagg agcttcgcca gctcctggag
     4441 cgcgccctgc gcctgggtga tcgagcgcat ctcgatccag tgccccagga gcatgatggt
     4501 cacgagcgtg gccagctccc accagagggc ctccgcgtcg atgagcctca gctgcaccac
     4561 ccaggagaag acgaaggcca cggtgatggc cagggcgatg aggaccatca tccccggcag
     4621 gcgagcccgg agctcgcgca cggccgcctg gaggaagacc aggcccccat agaggaagac
     4681 cgccgtccc
//
LOCUS       DASUTW010000136         3456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_561147, whole genome shotgun sequence.
ACCESSION   DASUTW010000136 DASUTW010000000
VERSION     DASUTW010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561147"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..718
                     /locus_tag="VF058_04545"
     CDS             50..718
                     /locus_tag="VF058_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207611.1"
                     /translation="MRGSRLRWLIPTVVLISIVLIPLLAWGQTPPPGEEPDVVRPWTW
                     WLAIPLALAVLGLVAFIALRYVRFSPRFFGKEEAPKIPERPRAYAGVNAPAYPAPALA
                     AAPAAAPAPAATATAEAPAPAQAPAAEPEPEPQPEPEPEPEPEPEPEAEPQPEAEPQP
                     EAEAPTPEPAADAQAPEEKAEPAGDADDVYERVLKEQLDKGVSPKVAEGRAKAAAHRA
                     RRSG"
     gene            721..1761
                     /locus_tag="VF058_04550"
     CDS             721..1761
                     /locus_tag="VF058_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX6207612.1"
                     /translation="MGAAAKLADWGTARRIGVTFSGEGPIIAPVDRARLSEDFAEAVS
                     RADALVADLTGLAIDGPATRPWVMTRDEWVRQNVRGFETLLDPVADRLTSGGDGPMAA
                     VRRKFLAAQVGGLLGYLSRRVLGQYDLFLPPDDRDLLYFVGPNVISVERRHRLQPEEF
                     RLWLSLHEVTHRFQFDGVPWLRGYLRGLVDEYVESLDLDPRRLLEAFRRAREEAARSP
                     AWRGVGILFLLMTPEQRDTFRRMQALMSLLEGHGNHVMHVLSEGRIRGAPRFERVLRE
                     RRRSAGAGRAFQRAIGFDAKVRQYGEGERFIAAVVERAGKEGFNRVWDGPERLPTLEE
                     VTDPEAWVRRVV"
     gene            1763..2797
                     /gene="tilS"
                     /locus_tag="VF058_04555"
     CDS             1763..2797
                     /gene="tilS"
                     /locus_tag="VF058_04555"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX6207613.1"
                     /translation="MAGRPPAVARVLERATRTIRRHEMLPPGRAVLVACSGGPDSICL
                     LHALHRLRRLLRIRLEVFHFDHRLHRTSGEHAAYVRRQARRLGLPFHLRAAGGGPARG
                     ESPEAWARLARYAALTEVATERGIERAAVAHTRDDQAETVLLGLVRGGGLEAVAGMDP
                     VATVPPLALTVVRPLLDVTREEVEAFCRSLRLRPREDPTNLDPRFLRNRVRHRTLPRL
                     ERDLDRNLRATLARVAANVREDAAYLDEVASVAAREVVEVDEGEVRMRVARLRALVPA
                     VASRVARDALRVAAAMAGSWTADVEAVHVEALLDLARGRPGRRIDLPGALMGRRDREY
                     VRLRPAGEAI"
     gene            2797..3396
                     /gene="hpt"
                     /locus_tag="VF058_04560"
     CDS             2797..3396
                     /gene="hpt"
                     /locus_tag="VF058_04560"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX6207614.1"
                     /translation="MGDGIGRASTLTGHVAEYEQDIERVLITEDEIQAKLKELGERIT
                     DDYRGHDLVLVGVLKGAFVLMADLSRHIRLPIQFDFMAISSYGAATKSSGVVRILKDL
                     DHEIEGRDVLVVEDIVDSGLTLQYLLRNLQARKPATLEVAALLLKTEVDRVPVTIRYT
                     GFEIPPEFVVGYGLDYAERYRNLPFVGTLKPQVYETLVE"
BASE COUNT          452 a         1263 c         1287 g          454 t
ORIGIN      
        1 acacgatcgc gggaggcccg tgaacgggga tttgttagaa tccgcgccga tgcgcgggtc
       61 ccgtctgcgg tggctgatcc ccacggtggt cctcatctcc atcgtgctga tccccctgct
      121 cgcctggggg cagacgccgc cccccggcga ggagcccgac gtggtccgcc cctggacctg
      181 gtggctcgcg atccccctcg ccctggcggt cctgggcctc gtggccttca tcgccctgcg
      241 gtacgttcgg ttctcccctc ggttcttcgg gaaggaggag gcgccgaaga tcccggagcg
      301 cccccgcgcc tacgccggcg tgaacgcgcc cgcctacccc gcccccgccc tcgccgccgc
      361 gccggcggcc gccccggccc cggcggcgac ggccactgcc gaggcccccg ccccggcgca
      421 ggcccctgcg gcggagcccg agcccgagcc acagcccgaa cccgaaccgg agccggagcc
      481 ggagcctgaa ccggaggccg agccacagcc cgaggccgag ccacagcccg aggccgaggc
      541 gccgaccccc gagcccgccg cggatgccca ggcccccgag gagaaggccg agcccgcggg
      601 cgacgccgac gacgtgtacg agcgtgtcct gaaggagcag ctcgacaagg gcgtctcccc
      661 caaggtggcc gagggacggg ccaaagccgc cgcccaccgc gcccgtcggt cgggctgagg
      721 atgggggccg cggcgaagct ggccgactgg gggacggccc gcaggatcgg cgtcacgttc
      781 tcgggagagg gcccgatcat cgcgcccgtc gaccgggcgc gcctgagcga ggacttcgcg
      841 gaggccgtgt cgcgggcgga cgcgctcgtc gccgacctca cggggctcgc gatcgacggg
      901 ccggccacgc gcccctgggt gatgacccgg gacgagtggg tccggcagaa cgtccgaggc
      961 ttcgagacgc tcctggaccc ggtcgccgac cgcctcacga gcggcgggga cggcccgatg
     1021 gccgccgtcc gccggaagtt cctggcggcc caggtggggg gcctgctcgg ctacctgagc
     1081 aggcgggtcc tgggccagta cgatctcttc ctaccccccg acgaccggga cctcctctac
     1141 ttcgtggggc cgaacgtgat ctcggtggag cggcgccatc ggctccagcc ggaggagttc
     1201 cggctgtggc tctcgctgca cgaggtgacg caccgcttcc agttcgacgg cgtgccgtgg
     1261 ctccggggct acctccgggg actcgtggac gagtacgtgg agtcgctgga cctggacccg
     1321 cggcgcctgc tggaggcgtt ccggagggcc cgcgaggagg cggctcggag ccccgcctgg
     1381 cgcggggtgg ggatcctctt cctcctgatg acgccggagc agcgcgacac cttccggagg
     1441 atgcaagcgc tgatgtcgct cctcgagggg cacggcaacc acgtgatgca cgtcctctcg
     1501 gagggccgga tccggggggc ccccaggttc gagcgagtgc tgcgggagcg gcgccggtcc
     1561 gcgggcgccg ggcgggcctt ccagcgagcg atcggcttcg acgccaaggt gcgccagtac
     1621 ggcgagggcg agcggttcat cgcggcggtc gtggagcggg ccgggaagga gggcttcaac
     1681 cgggtgtggg atgggcccga gcgcctcccc acgctggagg aggttaccga ccccgaggcc
     1741 tgggtccgcc gggtcgtctg acatggccgg gcgcccgccc gccgtcgcgc gggtgttgga
     1801 gcgagcgacc cggacgatcc gaaggcacga gatgctgccg cccggacggg cggtgctcgt
     1861 ggcctgctcc ggcggcccgg actcgatctg cctgctccac gcccttcacc gtctccggcg
     1921 tctgctcagg atccgcctgg aggtcttcca cttcgatcac cggctccacc ggacctccgg
     1981 cgagcacgcg gcctacgtgc ggcgccaggc ccggaggctc ggcctgccgt tccacctccg
     2041 ggcagcgggc ggcggaccgg ctcgcggaga gtccccggag gcctgggccc ggctcgctcg
     2101 atacgcggcc ctgaccgagg tcgccaccga gcgggggatc gagcgggcgg ccgtggccca
     2161 cacgcgggac gaccaggccg agaccgtcct gctgggcctg gtccgagggg gcggtctgga
     2221 ggcggtggcc ggcatggatc cggtggccac ggtgccgccg ctcgcgctga ccgtggtccg
     2281 gccgctgctc gatgtcactc gggaggaggt cgaggccttc tgccggtcgc tgcgcctccg
     2341 gccgcgcgag gaccccacga acctcgaccc gcgcttcctc cggaaccggg tccgacaccg
     2401 cacgctgcca cgtctcgagc gggacctgga ccggaacctg cgggccacgc tcgcccgggt
     2461 cgcggccaac gtccgagagg acgccgccta cctcgacgag gtggcgtctg tggccgcgcg
     2521 cgaggtcgtg gaggtggacg agggggaggt ccggatgcgc gtcgcgcgcc tccgagcgct
     2581 ggtgccggcg gtggcctcgc gggtggcgcg cgacgcgctc cgtgtggccg cggccatggc
     2641 gggctcctgg accgccgacg tcgaggcggt ccacgtcgag gcgctcctcg acctcgcgcg
     2701 cggccgcccc ggccgccgga tcgatctgcc cggcgccttg atggggcggc gggacaggga
     2761 gtatgttcgg ctcaggccgg cgggggaggc gatctgatgg gcgacggcat cggccgggcg
     2821 tctacactga cgggccacgt ggccgagtac gagcaggaca ttgagcgggt cctcatcacc
     2881 gaggacgaga tccaggccaa gctgaaggag ctcggcgagc gcatcaccga tgactaccgg
     2941 ggtcacgacc tcgtgctcgt gggcgtgctg aagggcgcgt tcgtgctgat ggccgacctc
     3001 tcccgacaca tccgcctccc gatccagttc gacttcatgg cgatctcgtc ctacggcgcc
     3061 gccacgaagt cgtccggggt ggtccgcatc ctgaaggacc tcgaccacga gatcgagggg
     3121 cgggacgtcc tcgtcgtcga ggacatcgtg gactcggggc tgaccctcca gtacctcctg
     3181 cggaacctcc aggcgaggaa gcccgccacc ctcgaggtcg ccgcgctcct cctcaagacc
     3241 gaggtggacc gggtcccggt cacgatcagg tacacggggt tcgagatccc ccccgagttc
     3301 gtcgtgggct acggcctcga ctacgcggag cgctaccgca acctcccctt cgtggggacg
     3361 ctgaagccgc aggtctacga gacgctcgtc gagtagccgg cgcgggccac cgccgcccgg
     3421 gcccgcagca ggaagaagaa ccccgccgcc cccacc
//
LOCUS       DASUTW010000137         3849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1337752, whole genome shotgun sequence.
ACCESSION   DASUTW010000137 DASUTW010000000
VERSION     DASUTW010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337752"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="VF058_04565"
     CDS             <1..1197
                     /locus_tag="VF058_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6207615.1"
                     /translation="LASGRLPGMRGFTTRALGGRREDLGDAVPVAPPIHPTATYAFED
                     PGVLADLIRRGKDVGYVYTRWHNPTRDLLERLMAELEGGERAVSFSSGMAAIVTTFAA
                     LAGRGDHVVSSPSLYGGSFSTLSKLLPRWGVEVTFAESHRTEEMLAVIRPETKLVYTE
                     TIGNPTLQVPDLEALGSGCRERGVPLVVDNTFASPYLCRPLELGAAVVVHSTTKYLGG
                     HSDLTGGIAVGDDELLHEVREMSVDLGGTANAWESWLTMRGIETLALRMDRHVENARA
                     LADLLEGHPAVARVWYPSLPSHPDREVAERILRAPGGMISFELTGGLEAGRRFQQSLE
                     VALVGPSLGSTKTLVVHPPTVTHTQMTKEQREALGITDGFVRVSTGIEDAEDILADFR
                     QALEKA"
     gene            1194..2261
                     /locus_tag="VF058_04570"
     CDS             1194..2261
                     /locus_tag="VF058_04570"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEX6207616.1"
                     /translation="MIRVAVVFGGRSAEHEVSCVSAASVIGAIDREGFEVVPVGIDKD
                     GRWMLLEGPPPRDPDADRLPAIEPGSGEPVTLAGAGEPALVRSSGERIPVDVVFPVLH
                     GPYGEDGTIQGLLEFAGIPYVGAGVLASAVAMDKSVARVLFRAAGLPLTPWLVTSRVE
                     WGEDAEAVEARAEALGVPLFVKPAALGSSVGVSKVKSLADLPGALEEAFRHGRRAVVE
                     AAVEDAREIECAILGNDEPVASVPGEIVPAGEFYDYRSKYLDDRTRLIVPADLSPETV
                     EEVQRMAVAAFQAVDAAGMARVDFLLGPRGLYLNEVNTIPGFTEVSMYPKMWEASGLP
                     YPDLIDRLIHLGLERHGGADV"
     gene            2254..3678
                     /locus_tag="VF058_04575"
     CDS             2254..3678
                     /locus_tag="VF058_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014987609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX6207617.1"
                     /translation="MSDPDPREQKEILDLAREEAERYLDALPDEPVRTQRADEAALAF
                     DGPLPEEGVGGPAAIRELLEGTDALIRSSGPRFFHWVIGGTTPAAMGADWLTSAYDQN
                     AGAWDSSPRSARLERVAVGWLRDLFGLPQSWGGVLTTGATMANFAGLACARSWWAERH
                     GVDVDVEGLGELPPVPVFSSGYVHPSATKALAMMGIGRERVKRLARDDAGRLDLGALE
                     RELVGLGGQPAIVIGNAGEVNTGDFDPIAAMADLCEEHGAWLHVDGAFGLFAAVSPRS
                     AHLVSGIERAHSVISDGHKWLNVPHDTGFAFLKDPSRLGRVFSVAAAYLPGPDEERPV
                     FFNLGPESSRRARSLAVWATLRAYGREGYRRIVERHLDLAQRVARYVDEAPDFELLAD
                     VPLNIVCFRYRPQDAPEDELNELNERIGRDVMADGRLYVGTTTFEGKAAFRPALVNWR
                     TAEDDVDLIANVIRELGERARASA"
BASE COUNT          510 a         1357 c         1436 g          546 t
ORIGIN      
        1 ctggcctcgg gtaggcttcc cgggatgcga ggcttcacca cgcgagcgct gggcgggcgg
       61 cgcgaggacc tgggggacgc ggtccccgtc gccccgccga tccaccccac cgccacctat
      121 gccttcgagg accccggggt cctggccgac ctgatccgcc gggggaagga cgtcggctac
      181 gtctacacgc ggtggcacaa ccccacccgc gacctgctcg agcgcctgat ggccgagctg
      241 gagggcgggg agcgggccgt ctccttctca tccggcatgg cggccatcgt cacgaccttc
      301 gccgccctcg cggggagggg agaccacgtc gtgtcgagcc cctcgctgta cggcgggagc
      361 ttctccaccc tctccaagct gctcccccgg tggggggtgg aggtgacgtt cgcggagagc
      421 caccggaccg aggagatgct tgccgtcatc cggcccgaga cgaagctcgt ctacaccgag
      481 acgatcggga acccgacgct gcaggtgccc gacctcgagg ccctcggctc ggggtgccgg
      541 gagcgcgggg tgccgctcgt cgtggacaac acgttcgcca gcccctacct ctgccgcccg
      601 ttggagctcg gcgccgcggt cgtcgttcac tcgaccacga agtacctcgg gggtcactcc
      661 gacctgacgg gggggatcgc ggtgggcgac gacgagctcc tgcacgaggt ccgcgagatg
      721 tcggtcgacc tcgggggcac cgcgaacgcc tgggagtcgt ggctcacgat gcgcggcatc
      781 gagacgctgg ccctccggat ggatcgccac gtagagaacg cccgggcgct ggccgacctc
      841 ctggaggggc acccggccgt ggcgcgcgtc tggtacccga gcctgccgtc gcacccggac
      901 cgcgaggtcg ccgagcggat cctccgggcg ccggggggga tgatctcgtt cgagctgacc
      961 ggcggcctgg aggcgggccg gaggttccag cagtccctgg aggtcgcgct cgtcgggccc
     1021 agcctgggga gcacgaagac gctcgtcgtc cacccgccga ccgtcaccca cacccagatg
     1081 acgaaggagc agcgcgaggc cctgggcatc accgacggct tcgtccgggt gtccacaggg
     1141 atcgaggacg ccgaggacat cctggcggac ttccgccaag cgctggagaa ggcgtgatcc
     1201 gcgtcgccgt cgtcttcggc ggccggtccg cggagcacga ggtctcgtgc gtctcggccg
     1261 cctccgtgat cggcgcgatc gaccgcgagg gcttcgaggt cgtgcccgtc ggcatcgaca
     1321 aggacggccg ctggatgctg ctcgagggcc cgccgccccg agatccggac gccgaccggc
     1381 tgcctgcgat cgagccgggg agcggggagc ccgtcacgct cgccggcgcg ggcgagcccg
     1441 ccctcgtccg ctccagtggc gaacggatcc cagtggacgt cgtcttcccc gtgctccacg
     1501 gcccctatgg cgaggacggc acgatccagg ggctcctcga gtttgccggg atcccgtacg
     1561 tgggggcggg ggtgctggcc tcggcggtgg cgatggacaa gtcggtggcc agggtgctgt
     1621 tccgcgccgc cggcctcccc ctgacgccgt ggctcgtgac ctcgcgcgtg gagtggggag
     1681 aggacgccga ggccgtggag gcccgggccg aggcgctcgg cgtgccgctg ttcgtcaagc
     1741 cggccgccct cgggtcgtcg gtgggggtgt cgaaggtgaa gtcgctggcg gacctccccg
     1801 gcgcgctcga ggaggcgttc cgccacgggc gacgcgccgt cgtggaggcg gcggtcgagg
     1861 acgcccggga gatcgagtgc gcgatcctgg ggaacgacga gccggtggcc tccgtccccg
     1921 gggagatcgt gcccgccggg gaattctacg actaccgttc caagtacctg gacgaccgca
     1981 cgcgcctgat cgtgccggct gacctctcgc ccgagacggt ggaggaggtc cagcggatgg
     2041 cggtcgccgc gttccaggcc gtggacgccg cggggatggc ccgcgtggac tttctcctgg
     2101 gccctcgcgg tttgtacctg aacgaggtga acacgatccc agggttcacc gaggtgtcga
     2161 tgtacccgaa gatgtgggag gcctcgggcc tcccgtaccc ggatctcatc gaccgcttga
     2221 tccacctggg cctggagcgc cacggaggtg ccgatgtctg accccgatcc gcgagagcag
     2281 aaggagatcc tcgacctggc ccgggaggag gccgagcgct atctcgacgc gcttcccgac
     2341 gagccggtgc ggacccagcg agcggacgag gccgccctgg ccttcgacgg gcccctgccc
     2401 gaggagggcg tgggcgggcc ggccgcgatc cgcgagctcc tcgaggggac cgacgccctg
     2461 atccggtcgt ccggcccccg cttcttccac tgggtgatcg gcgggacgac gcccgcggcg
     2521 atgggggccg actggctgac ctccgcctac gaccagaacg ccggggcctg ggactcctca
     2581 ccacggtccg cccggctgga gagggtggcg gtgggatggc tccgcgacct cttcggtctg
     2641 ccgcagagct ggggcggggt gctcacgacg ggcgcgacga tggccaactt cgccggcctg
     2701 gcgtgcgcca ggagctggtg ggccgagcgg cacggggtcg acgtggacgt cgaagggctg
     2761 ggcgagctgc cccccgtccc cgtgttctcc agcgggtacg tgcaccccag cgccacgaag
     2821 gcgctggcga tgatggggat cggccgggag cgggtgaagc gcctggcgcg cgacgacgcg
     2881 gggcgcctcg acctcggtgc gctggagcgc gagctggtcg gcctcggggg ccagccggcg
     2941 atcgtgatcg ggaacgcggg ggaggtcaac acgggcgact tcgatccgat cgccgccatg
     3001 gccgacctct gcgaggagca tggggcctgg ctgcacgtgg acggcgcgtt cgggctcttc
     3061 gcggcggtct cgccgcggtc ggcgcacctc gtgagcggga tcgagcgcgc gcactccgtg
     3121 atctcggacg gccacaagtg gctcaacgtc ccgcacgaca ccggcttcgc gttcctcaag
     3181 gacccctcgc ggctgggccg cgtgttctcg gtcgcggccg cctacctccc gggccccgac
     3241 gaggagcggc ctgtgttctt caacctcggg ccggagtcgt cgcgccgggc tcgctcgctg
     3301 gccgtctggg ccaccctgcg cgcgtacggc cgcgagggct accggcgcat cgtcgagcgc
     3361 cacctggacc tcgcccagcg tgtcgcccgc tacgtggacg aggcgcccga cttcgagctc
     3421 ctggcggacg tcccgctgaa catcgtctgc ttccgctacc ggccacagga cgcccccgag
     3481 gacgagctca acgagctgaa cgagcggatc ggacgcgacg tgatggcgga cggccgcctc
     3541 tacgtgggga cgacgacctt cgaggggaag gcggccttcc gtccggcgct cgtcaactgg
     3601 cggaccgccg aggacgacgt ggacctgatc gcgaacgtga tccgcgagct gggggagcgg
     3661 gctcgggcgt cggcctagtc aggcgacccg acgctccgtc tcggggaccc ggtcggccga
     3721 ctcgcgctcc gagccgcacc tcgcgcactc ccatcgcacg acctcgagct cctgagtctc
     3781 ctcgagggcc aggtaggaat ccgcgacgcg cagctcggcc tggcagctct cgcagctctc
     3841 ccgcatccg
//
LOCUS       DASUTW010000138         2817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_431350, whole genome shotgun sequence.
ACCESSION   DASUTW010000138 DASUTW010000000
VERSION     DASUTW010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431350"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1309
                     /locus_tag="VF058_04580"
     CDS             170..1309
                     /locus_tag="VF058_04580"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6207618.1"
                     /translation="MSPLLFAYLAFGSFWGTWVVVFADFLRHHGLTPGRVSIWWTLLS
                     LVAILVMTFLAPRMEPLPRHWTVGWAMAAHAVGSALMVLTSAGWLPIAFMALGFGTGL
                     VDVFVNAAAYEIEERSRSPVLQWVHAAYGAGGVVGSLGAGIAVTAGASFRAVVLGSVV
                     FQIGAAITALRSRGLRAAHTPREPGRVSLAVFRKARFLLVPALVVAAAFFIEGSMDVW
                     SVIFLRETLGASTLAGAAGFAAFAGAITIGRVVGAGSFRLGYARTILLSGLGSLAAGL
                     IAVATQSPVVASLAFLGLGLALASAAPAAYGMAEEAGPASGLAVGAITTVGYTGFVVG
                     PPIMGWLADAVSLRATMVALVATTLGILAGGLLALRRERAATPPG"
     gene            complement(1293..2732)
                     /locus_tag="VF058_04585"
     CDS             complement(1293..2732)
                     /locus_tag="VF058_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6207619.1"
                     /translation="MADELRNYVDGEWVDARDGGRFDVVDPATEEVIATAPDSQRADA
                     EAAISAARRAFDAGPWPRSTERERERVLLRAAEIVRREAERLAELETRDSGKPLAEAR
                     EDIEEAAFMFEYYGGWATKVMGEIPPVGPDALSLVVKEPVGVAGLITPWNYPLLMAVQ
                     KVAPALAAGCTFVLKPAEQTPLTAFELPGILEEAGVPPGVVNVIAGFGPSAGAPLVEH
                     PDVDKVGFTGSVEVGKEIMRTGADTLKRVTLELGGKSPNIIFADASFEEALEGTAAGI
                     FGNQGEICSAGSRIFVERSIYDETLDAMRERARAVRLGSGLDEGTTMGPLVSEEQQER
                     VLSYIESGKAEGAKLATDEPPAFDSGYFVPPTVFYDVTNDMTIAREEIFGPVAAVLPF
                     DDPEEVVRLSNDTTYGLAAAVWTRDITKALRTARALRAGVVWINDSQPAPTEAPWGGY
                     KQSGIGRELGPQGLEDYLETKHIYVNLAE"
BASE COUNT          326 a         1016 c         1011 g          464 t
ORIGIN      
        1 gagggccctg gaggcgggct gacggtcgag gaagatccgc tcctgctcct cgatcagctg
       61 atcgagacga tccatgctcg ctccttcatc tttgaacggc cattcaatat catacggacc
      121 gatcgaggag ggggcaatgg ccaggacggg agcgaggagc cgagcgggga tgagcccgct
      181 gctcttcgcc tacctcgcct tcgggtcgtt ctgggggaca tgggtggtgg tgttcgccga
      241 cttcctccgg caccacggcc tcacgccggg cagggtgagc atctggtgga cgctgctctc
      301 gctcgtggcg atcctcgtga tgacgttcct ggcgccgcgc atggagccgc tcccccgcca
      361 ctggacggtc ggctgggcga tggcggcgca cgcggtcgga tcggcgctca tggtcctgac
      421 ctccgcggga tggctgccca tcgccttcat ggcgctgggc ttcgggacgg gcctcgtcga
      481 cgtcttcgtg aacgccgccg cctacgagat cgaggagcgc tcgcgttcgc ccgtcctcca
      541 gtgggtccac gccgcctacg gggccggcgg ggtcgtgggg tccctcggtg ccgggatcgc
      601 cgtcacggcg ggcgcttcgt tccgggccgt cgtgctcggc tcggtggtgt tccagatcgg
      661 tgccgcgatc accgcgcttc ggtcccgagg gctgcgcgcc gcccacaccc cgcgggagcc
      721 cggccgcgtc tccctggccg tgttccggaa ggctcgcttc ctcctcgtcc ccgcgctcgt
      781 cgtggccgcc gccttcttca tcgagggctc gatggacgtg tggtcggtga tcttcctccg
      841 cgagacgctc ggggcgagca cgctggcagg cgcggcgggg ttcgccgcct tcgccggggc
      901 gatcacgatc gggagggtcg tgggggcggg ctcgttccgg ctcgggtacg cccgcacgat
      961 cctgctgtcg ggcctggggt cgctggcggc cgggctgatc gcggtggcca cgcagagccc
     1021 cgtggtggca agcctcgcgt tcctcgggct ggggctggcc ctggcctcgg cggccccggc
     1081 cgcctacggg atggccgagg aggcgggacc cgcgtcgggg ctcgcggtgg gagcgatcac
     1141 cacggtgggc tacacggggt tcgtggtggg gccgccgatc atgggctggc tcgccgacgc
     1201 ggtgagcctg cgcgcgacga tggtcgccct cgtcgccacc acgcttggga tcctggccgg
     1261 ggggctgctc gccctccgcc gggagcgcgc cgctactccg ccaggttgac gtagatgtgc
     1321 ttcgtctcga ggtagtcctc gaggccctgc ggtccgagct cgcgcccgat ccccgactgc
     1381 ttgtagccgc cccacggggc ctcggtcggc gcgggctgtg agtcgttgat ccataccacg
     1441 cccgcccgca gggctcgcgc ggtccgaagg gccttcgtga tgtcacgggt ccagaccgcc
     1501 gcggccagcc cgtaggtcgt atcgttggag agccgcacga cctcctccgg gtcgtcgaac
     1561 ggcaggacgg cggccaccgg cccgaagatc tcctctcggg cgatcgtcat gtcgttcgtc
     1621 acgtcgtaga agaccgtggg tgggacgaag tagccgctgt cgaaggcggg cggttcgtcg
     1681 gtggcgagct tcgcgccctc cgccttcccc gactcgatgt acgagagcac ccgctcctgc
     1741 tgctcctcgc tcaccagcgg gcccatcgtg gtgccctcgt cgagcccgct ccccaaccgg
     1801 acggcccggg cccgctcgcg catcgcatcc agtgtctcgt cgtagatcga acgctccacg
     1861 aagatgcggc tgccggccga gcagatctca ccctggttgc cgaagatccc cgcggccgtc
     1921 ccctccagcg cctcctcgaa cgaggcgtcg gcgaagatga tgttcggcga cttcccgccg
     1981 agctcgagcg tgacgcgctt cagcgtgtcg gcgccggtcc gcatgatctc cttccccacc
     2041 tcgaccgagc cggtgaagcc caccttgtcc acgtcggggt gctcgacgag gggcgcgccg
     2101 gcgctgggcc cgaaccccgc gatcacgttg accacgccgg gcgggacgcc ggcctcctcc
     2161 aggatccctg ggagctcgaa ggcggtcagc ggcgtctgct ccgcgggctt caggacgaac
     2221 gtgcacccgg cggccagcgc gggcgccacc ttctggacgg ccatcagcag cggatagttc
     2281 cacggggtga tgaggcccgc cacgccgacg ggctccttga cgacgaggct gagggcgtcg
     2341 gggccgaccg gggggatctc gcccatgacc ttcgtggccc agccgccgta gtactcgaac
     2401 atgaaggcgg cctcctcgat gtcctcgcgg gcctcggcga ggggcttgcc ggagtcgcgc
     2461 gtctcgagct cggcgaggcg ctcggcctcg cggcggacga tctcggccgc ccgcaggagc
     2521 acgcgctcgc gctcgcgctc ggtggaccgg ggccaggggc ccgcgtcgaa ggccctgcgg
     2581 gcggccgaga tcgcggcctc ggcgtcggcc cgctgagagt ccggggccgt ggcgatgacc
     2641 tcctcggtgg cggggtccac cacgtcgaac ctcccgccgt cccgtgcgtc cacccactcg
     2701 ccgtcgacgt agttgcgaag ctcgtccgcc atcacgctcc ctccgtcagc cccatcgcct
     2761 tcgccggcag ggtgggcacc gggcccgtgg ccttccccgc caggtatccg tgcgcca
//
LOCUS       DASUTW010000139         2616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_302054, whole genome shotgun sequence.
ACCESSION   DASUTW010000139 DASUTW010000000
VERSION     DASUTW010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302054"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1192)
                     /locus_tag="VF058_04590"
     CDS             complement(161..1192)
                     /locus_tag="VF058_04590"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX6207620.1"
                     /translation="MSRVAVIGAGSWGTAFARIPAEKDVETVLWARRPELAVEIGSRH
                     ENPDYLPGTTLPPSLTSTSDLEEAAARAEVLVMAVPSHAFREVFREVGPFLGSEAPVV
                     SLTKGIEQGSLRRMTEVMAEEAELDPRRVAVVSGPNLAKEVVAGMPSATVVACVDEER
                     AGHLQELFMAPYFRVYTNPDVIGVEMAGALKNVVAIAAGIADGMGFGDNSRASLITRG
                     LAEITRLGVKLGGAPLTFAGLAGMGDLVATCISRLSRNRTLGEALGRGRSLEEIVGEM
                     RMVAEGVKSSRPVLALADREGVEMPIAEHVVKVLYEGVSPADMAASLMTRAARPELHG
                     IDPGPSGLG"
     gene            complement(1189..1863)
                     /locus_tag="VF058_04595"
     CDS             complement(1189..1863)
                     /locus_tag="VF058_04595"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX6207621.1"
                     /translation="MKRVEPWFRFAETVLIPPIALWFRWRFEGLERIPREGPVLVAAN
                     HISYLDPLAHGYFIEKARRRPRFLAKTELYGNPIVGAVLRGARQIPVRRGSGEHAPVE
                     SALKALRAGEVVVVYPESTVTSRADFSPMQGKTGIARLALAARVPVLPIAVWGSQRVW
                     QRGGARSLRFGRPIWVKAGAPLDLSAHEEGADDPTVLRSATQQVMDQLTVLVEDLRAR
                     YPKKWA"
     gene            1929..2573
                     /gene="cofC"
                     /locus_tag="VF058_04600"
     CDS             1929..2573
                     /gene="cofC"
                     /locus_tag="VF058_04600"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEX6207622.1"
                     /translation="MRVIAVPIKDLSRAKGRLSGILSPVERAALTLAMLEDVLAACVE
                     MPGWQTWVISPDPSALQQAARMRARPVEERERGLVSAIRQVEEEAANADALAVVLADL
                     PLLTPAALMRALQTLGSVVAAPADSDSGTNLLLRRPPDAIPPRFGTDSFRKHREAAEL
                     HDIPFAEVRLPQLAFDLDRPADLTALLASSSRSRTKATCLEMGLPERLAVRKQA"
BASE COUNT          370 a         1035 c          865 g          346 t
ORIGIN      
        1 gacgtccttc ccccggcgga tcaggtcggc caggacctcg gggtcctcga acgcgtaggt
       61 ggcggtgggg tggatcggcg gggcgacggg ggccgcgtcc cccaggtcct cgcgccgccc
      121 gcccagcgct cgcgtggtga agcctcgcat cccgggaagc ctacccgagg ccagagggtc
      181 ccgggtcgat gccgtggagc tcggggcgcg ccgcccgcgt catcagcgag gcggccatgt
      241 ccgcgggcga gaccccctcg tacaggacct tcaccacgtg ctcggcgatc ggcatctcca
      301 ccccctcccg atcggccagc gccagcaccg gccgactcga cttcacgccc tcggccacca
      361 tccgcatctc ccccacgatc tcctccaggc tccggccgcg gcccagggcc tcacccagcg
      421 tgcggttccg ggatagccgg gagatgcacg tggccaccag gtcgcccatc ccggcgaggc
      481 cggcgaacgt gagcggggct ccgcccagct tcacgcccag ccgggtgatc tctgccaggc
      541 cgcgggtgat cagggaggcg cgggagttgt cgccgaaccc catcccgtcg gcgatccccg
      601 cggcgatcgc gacgacgttc ttgagcgcgc cggccatctc caccccgatc acgtccgggt
      661 tcgtgtacac ccggaagtac ggggccatga acagctcctg gagatggccg gcgcgctcct
      721 cgtcgacgca cgccaccacg gtggccgagg gcatcccggc cacgacctcc ttggccaggt
      781 tcgggcccga gaccaccgcc acccggcggg gatcgagctc ggcctcttcg gccatcacct
      841 cggtcatccg ccgcagcgac ccctgctcga tccccttcgt cagcgagacc acgggggcct
      901 ccgacccgag gaacgggccc acctcgcgga acacctcccg gaaggcgtgg gacgggacgg
      961 ccatcaccag gacctcggcc cgagccgccg cctcctcgag atccgaggtc gacgtcagcg
     1021 acggaggcag cgtcgtcccc ggcaggtagt cggggttctc gtggcgagac ccgatctcga
     1081 cggcgagctc ggggcggcgg gcccacagca ccgtctcgac gtccttctcc gccgggatgc
     1141 gcgcgaaggc cgtcccccag gatccggccc cgatgaccgc gacccggctc acgcccactt
     1201 cttcgggtat cgggcccgga ggtcctccac gagcaccgtc aactggtcca tcacctgctg
     1261 cgtggcgctc ctgagcacgg tcgggtcgtc ggccccctct tcgtgagccg agaggtcgag
     1321 cggggccccg gccttcaccc agatcggtcg gccgaagcgg aggctccgcg cgccgcctcg
     1381 ctgccagacc cgctgggagc cccagacggc gatcggcagg acggggacgc gggcggccag
     1441 cgccagccgg gcgatgcccg tcttcccctg catcggcgag aagtcggccc tgctggtcac
     1501 ggtcgactcc gggtagacga ccacgacctc gccggcccgc agcgccttca gcgccgactc
     1561 caccggcgcg tgctcccccg acccgcgccg cacggggatc tggcgggcac cccggagcac
     1621 cgcccccacg atcgggttcc cgtagagctc ggtcttcgcc aggaagcggg gacgccgcct
     1681 cgccttctcg atgaagtagc cgtgcgccag ggggtcgagg taggagatgt ggttggccgc
     1741 cacgaggacc ggcccctcgc gcgggatccg ctccagcccc tcgaagcgcc accggaacca
     1801 cagcgcgatg ggcgggatca gcaccgtctc cgcgaaccgg aaccagggct cgacccgctt
     1861 catgggcgcc atcctaccgg cgggggatgg ttccatggaa cacccggacc cgctagcatg
     1921 actcccccat gcgcgtcatc gccgttccca tcaaggacct ctccagagcg aagggacgac
     1981 tctccgggat cctctccccg gtggagcgcg ccgcgctcac cctggccatg ctcgaggacg
     2041 tcctcgccgc ctgcgtcgag atgcccggct ggcagacctg ggtgatctcg cccgacccct
     2101 cggccctcca gcaggcggcg cggatgcggg cacgcccggt cgaggagcga gagcgcggcc
     2161 tggtctcggc gatccgtcag gtcgaggagg aggccgcgaa cgccgacgcc ctggccgtcg
     2221 tgctggccga cctccccctc ctgacgcccg ccgccctgat gcgcgcgctc cagacgctgg
     2281 gcagcgtggt ggccgctccc gccgattcgg actccggcac gaacctcctc ctccggcgtc
     2341 cccccgacgc catcccccct cgattcggca ccgactcgtt caggaagcac cgcgaggcgg
     2401 ccgagctcca cgacatcccg ttcgccgagg tccggctccc ccagctcgcc ttcgacctcg
     2461 accggcccgc ggacctcacc gccctgctcg cctcctcctc gcgcagccgg acgaaggcga
     2521 cttgcctcga gatgggcctc ccggagagac ttgccgtccg gaagcaagcc tgaggagcgc
     2581 gcgtggctca aggaacgatc aagacgtacg accgcg
//
LOCUS       DASUTW010000140         3330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1727588, whole genome shotgun sequence.
ACCESSION   DASUTW010000140 DASUTW010000000
VERSION     DASUTW010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727588"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..2158
                     /gene="infB"
                     /locus_tag="VF058_04605"
     CDS             2..2158
                     /gene="infB"
                     /locus_tag="VF058_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX6207623.1"
                     /translation="MRIHELAKELDVKSKELIGVLEEMGYSGLTASSSVPDEAVPRLR
                     ASGGKAVPAGKPKVVTEEPVAPTRRKAPSKKAPTTKAARTKATTTKAKPAQKTEAAPT
                     EAEAPAEPRAEPAPAEPAAEPAAAAPPAPEPEVERVKIARGSSVKEVADKLGTEPTSL
                     IRKLLELGQMVTITQSLPDDAIELVAEDLGRPVEIVAPEEDLEPEDDDLDPERMTLRP
                     PVITVMGHVDHGKTLLLDAIRQTDVVSGEAGGITQAIGAYQVRQDGSEITFIDTPGHE
                     AFTAMRARGAQVTDIAVLVVAADDGVMPQTIEAIDHAAAAAVPIVVAVNKIDKPEADP
                     MRVRQQLAEVGIQPSEWGGDYEFVDVSAKSGEGIDKLLETLLLVSELQELKADSGAAS
                     RGIAIEAHLDRGRGPVATVLVQQGTLRVGDAIVSGAAHGRVRAMFDEHAAPVEEAGPG
                     KPVQVLGWSSPPEAGDDFRVAEDEREARRIAQEREARQRAAEIVAKRPAQTLEEILAT
                     AAREGEIADLNLVLKADTRGSLEALADALEKLSGDEVRVRILHQSVGAIAESDVRLAQ
                     ASSAVVVGFNVVPDPNARAAAEQEGVDIRTYRVIYEAVDQIKQAMSGLLTPGEEEVAL
                     GRAEVRAIFQVPRAGRAAGCYVTQGTITRNARARLVRDGVVVYDGRIASLRRFKDDVR
                     EVQEGFECGIGLEDFQDVKEGDVIEAYEVREVARSL"
     gene            2170..2463
                     /locus_tag="VF058_04610"
     CDS             2170..2463
                     /locus_tag="VF058_04610"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX6207624.1"
                     /translation="MLVALTAFDLRIPLAGSLKEKRRVVKSLTAGLRQRFNVAVAEVD
                     HQDLWRRATLAVSAVGGEGYQIKKVMHEVERYLDRQPEIEVISSHTSLHGHDD"
     gene            2456..2806
                     /gene="rbfA"
                     /locus_tag="VF058_04615"
     CDS             2456..2806
                     /gene="rbfA"
                     /locus_tag="VF058_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX6207625.1"
                     /translation="MTDGARPVRVAEEFRKVLAEEVQRLKDPRVGFVTITGVDVTPDL
                     RQAVVYYTVLGEERDRRATAAGLRSAAPRLRAELGRQVRLKFLPELRFEEDQAVVEGD
                     RVDELLRRIHEEET"
     gene            2803..>3330
                     /locus_tag="VF058_04620"
     CDS             2803..>3330
                     /locus_tag="VF058_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX6207626.1"
                     /translation="MTLDEKTMARAAQVLDEAEDVALVCHVNPDPDAMGSMLGLAGFL
                     ARRGKKVAATWPNDPAERPRWVDALGNAELIVTPKEMPKEPSVLVTLDAAAANRLDGM
                     IHLLERAKVSICIDHHRSNEGFGTINLIDPDASSTAEIVFRLIERMGGGMSDEDAKSL
                     YAGVVTDTGRFQYEAT"
BASE COUNT          536 a         1116 c         1263 g          415 t
ORIGIN      
        1 agtgaggatc cacgagctgg cgaaggagct ggacgtcaag agcaaggagc tcatcggcgt
       61 cctcgaggag atggggtact cgggcctgac ggcctcctcc agcgtgcccg acgaggccgt
      121 cccccgcctg cgggcctccg gggggaaggc ggtccccgcg gggaagccca aggtcgtcac
      181 cgaggagcct gtcgccccga cccggaggaa agctccctcc aagaaggccc cgaccacgaa
      241 ggctgctcgc acgaaggcca cgaccacgaa ggccaagccg gcccagaaga ccgaggccgc
      301 acccaccgag gcggaggcgc cggcggagcc gcgggccgag cccgctcccg ccgagccggc
      361 ggccgagccc gcggccgcgg ctccgccggc gccggagccc gaggtcgagc gggtcaagat
      421 cgcccgcggc tccagcgtga aggaggtcgc cgacaagctg gggacggagc ccacctcgct
      481 catcaggaag ctcctggagc tcggccagat ggtgacgatc acccagtcgc tccccgacga
      541 tgcgatcgag ctcgtggcgg aggacctggg tcggcccgtg gagatcgtgg cccccgagga
      601 ggacctcgag cccgaggacg acgacctcga ccccgagcgc atgaccctca ggccccccgt
      661 gatcaccgtc atgggccacg tcgaccacgg gaagaccctc ctgctcgacg cgatccgtca
      721 gactgacgtg gtctcgggcg aggcgggcgg catcacccag gcgatcgggg cctaccaggt
      781 gcggcaggac ggcagcgaga tcacgttcat cgacacaccg ggccacgagg ccttcaccgc
      841 gatgcgggcc cggggcgccc aggtcaccga catcgccgtc ctggtcgtgg cggccgacga
      901 cggcgtcatg cctcagacga tcgaggcgat cgaccacgcg gcggccgccg cggtccccat
      961 cgtcgtggcc gtgaacaaga tcgacaagcc cgaggccgat ccgatgcgcg tgcgccagca
     1021 gctcgccgag gtcggcatcc agccctcgga gtggggcggc gactacgagt tcgtggacgt
     1081 ctccgccaag agtggcgagg ggatcgacaa gctgctcgag acgctcctcc tcgtctcgga
     1141 gctccaggag ctgaaggccg actcgggggc ggcctccagg gggatcgcca tcgaggctca
     1201 cctggacagg gggcgtggac cggtggccac cgtgctggta cagcagggga ccctgcgggt
     1261 cggcgacgcg atcgtctccg gggccgccca cgggcgcgtt cgggcgatgt tcgacgagca
     1321 cgccgccccg gtggaggagg ccgggccggg gaagccggtc caggtgctcg gctggtcgtc
     1381 cccccccgag gcgggcgacg acttccgggt ggccgaggac gagcgagagg cgaggcggat
     1441 cgcccaggag cgggaggcgc gtcagcgcgc cgccgagatc gtggccaagc ggccggctca
     1501 gaccctcgag gagatcctgg cgacggcggc tcgggagggc gagatcgcgg acctcaacct
     1561 cgtgctgaag gcggacacgc gcggctcgct ggaggcgctc gccgacgccc tcgagaagct
     1621 ctcgggcgac gaggtccgcg tccgcatcct ccaccagagc gtcggggcga tcgccgagag
     1681 cgacgtccgg ctggcccagg cttcgagcgc ggtcgtggtc gggttcaacg tcgtgcccga
     1741 cccgaacgcg cgcgcggcgg ccgagcagga gggtgtggac atccgcacct accgggtgat
     1801 ctacgaggcc gtggaccaga tcaagcaggc gatgtcgggc ctgctcaccc ccggcgagga
     1861 ggaggtggcg ctgggccggg ccgaggtccg ggcgatcttc caggtgccgc gagccggccg
     1921 ggcagccggc tgctacgtca cccagggcac gatcacgcgg aacgctcggg cccgactggt
     1981 tcgggacggt gtcgtcgtct acgacgggcg gatcgcgagc ctgcgccgct tcaaggacga
     2041 cgtccgcgag gtccaggagg ggttcgagtg cggcatcggc ctcgaggact tccaggacgt
     2101 gaaggagggg gacgtcatcg aggcctacga ggtccgcgag gtcgcgcgct ccctctaggg
     2161 gtggtcgcga tgctcgtggc gctcacggcc ttcgatctcc ggatccccct cgccggctcg
     2221 ctcaaggaga agcgacgggt ggtgaagtcc ctcacggccg gcctccgcca gcgcttcaac
     2281 gtggcggtgg ccgaggtcga ccaccaggac ctgtggcggc gggcgacgct cgccgtgtcg
     2341 gcggtgggcg gggaggggta ccagatcaag aaggtgatgc acgaggtgga gcgatacctt
     2401 gaccgtcagc cggagatcga ggtgatctcg agccatacga gcctccacgg ccacgatgac
     2461 tgacggcgct cgccccgtgc gcgtggccga ggagttccgg aaggtcctgg ccgaggaggt
     2521 ccagcgcctg aaggatcccc gggtggggtt cgtcacgatc accggcgtcg acgtcacgcc
     2581 cgatctccgc caggcggtcg tctactacac ggtgctgggg gaggagcggg accggagagc
     2641 gaccgccgcg gggctccggt cggcggcccc gcggctgcgg gcggagctgg ggcggcaggt
     2701 ccggctgaag ttcctcccgg agctgcggtt cgaggaggac caggccgtcg tcgagggtga
     2761 tcgcgtcgac gagctgctgc ggaggatcca cgaggaggag acgtgacgct ggacgagaag
     2821 acgatggctc gggccgcgca ggtcctcgac gaggctgagg acgtggcgct cgtgtgtcac
     2881 gtcaaccccg accccgacgc catgggctcg atgctcgggc tggccggctt cctggcgagg
     2941 cgggggaaga aggtggccgc gacctggccc aacgacccgg ccgagcggcc gcgatgggtg
     3001 gacgcgctgg gcaacgccga gctcatcgtc accccgaagg agatgccgaa ggagccctcg
     3061 gtcctcgtca ccctggacgc cgccgccgcc aaccggctcg acgggatgat ccacctgctg
     3121 gagcgggcga aggtatcgat ctgcatcgac catcaccgct cgaacgaggg cttcgggacg
     3181 atcaacctca tcgatcccga cgcctcctcg acggcggaga tcgtcttccg gctgatcgag
     3241 cgcatgggag gcgggatgtc ggacgaggac gcgaagtcgc tgtacgcggg cgtcgtgacc
     3301 gacaccgggc ggttccagta cgaggcgacc
//
LOCUS       DASUTW010000141         7739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1858808, whole genome shotgun sequence.
ACCESSION   DASUTW010000141 DASUTW010000000
VERSION     DASUTW010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7739
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1858808"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VF058_04625"
     CDS             <1..510
                     /locus_tag="VF058_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX6207627.1"
                     /translation="RGLVYDRDEHYDVTSAFIKSMRGSDADASLFWLARMLEAGEDPR
                     FIARRMVIFASEDVGLADPVALQVAVAAAQAVEFVGLPEVRLNLAHAAVYLARAPKSN
                     AVIRAIGKADEAQRRAGRVPTYLRDAHYAGARELGHGEGYAYPPSDPDGAEGQRHLPE
                     GVEGGFLDE"
     gene            568..921
                     /locus_tag="VF058_04630"
     CDS             568..921
                     /locus_tag="VF058_04630"
                     /inference="COORDINATES: protein motif:HMM:NF017876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEX6207628.1"
                     /translation="MSAGDWALIVLAVFWAVLVVFLALVLVQLVRVLESTKILIDGIR
                     SETVPLLGEVTTSVKGVNRELDRVDDIVVSAASITKSAERLTSVVERAISSPLVKAIA
                     FGAGASRAFRRLRGS"
     gene            921..1151
                     /locus_tag="VF058_04635"
     CDS             921..1151
                     /locus_tag="VF058_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207629.1"
                     /translation="MKRIFWLAMGLGAGVTAAVMLSRWMRRQQERLSPAAIGEQVGET
                     ARDLGRLLRAALDEGREEMRRVEAEIRSSIPE"
     gene            1171..3786
                     /gene="alaS"
                     /locus_tag="VF058_04640"
     CDS             1171..3786
                     /gene="alaS"
                     /locus_tag="VF058_04640"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX6207630.1"
                     /translation="MEGAEIRRRFLRHFEERDHTIVPSSSLIPNDPTLLLTTAGMVQF
                     KPYFLGEQEPPYPRAASAQKSFRTTDIELVGHTDRHLTFFEMLGNFSFGDYFKEEAVR
                     LAWDLVTEGFGIDPDRLWATVYQTDDEAEEIWKAYLPADRVVRRGRADNFWDMGVAGP
                     CGPCSELYVDRGPEHGVEGGPEADEDRYLEIWNLVFMQNECDADIEVAGELPRRNIDT
                     GMGLERMALVLQEAGSAFETDLLRPLLEEAERLADRRYGADEDVDVSLRILAEHGRAT
                     TFLIADGVVPSNEGRGYVLRRMLRRVVSHARRLGMEGVVLRPLVERTVEMLGGAYPEL
                     VERRAFVLQVASSEEEHFARTLVQGMELFEREAERARGAGERAFPGEAAFLYHDTYGF
                     PIELTQELAREHGLEVDEAAFQRLMEEQRARARRAAKGGPDDRVLLEVARAAGRTEFV
                     GYAEAAAEGKVLALVRKGERVEAGGEGEEIELFLDRTPFYAEGGGQVGDAGVIRTDTG
                     VVSVADTVPGPGEVIVHRGTVTEGEIRADQEAHAEIDLPRREATARSHTGTHVLHWTL
                     RHLLGEHARQAGSLVAPGRLRFDFTHFEGLSPDLLEELEATANARLGEDAAVRAYETT
                     FEYARSQGALALFGEQYGELVRVVEIGDYSVELCGGTHVAHTGEVALMVFGSEGSIGS
                     GLRRVEALVGPDALAHVHAERRLLEELTEILGAQDPRQAVERAKRAVVRIKELESQLG
                     ELRQQERGEVVDSLLRETADVGGVSLVVARLDEDPGTLREMALKLRDRLQGGPGAAVL
                     AAADGRKATLVASATPELIDRGVTAPGLLEDAARAVGGGAGGKDHLAFAGGGAPDALD
                     EALRGLPERLVALLG"
     gene            3786..4211
                     /gene="ruvX"
                     /locus_tag="VF058_04645"
     CDS             3786..4211
                     /gene="ruvX"
                     /locus_tag="VF058_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018177393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX6207631.1"
                     /translation="MTAPGRVLALDVGKARMGVAVSDPDRRVALPAGTIRVVGGIQDL
                     RAVARLVERYEAAEVVVGHPLSLSGERGSAARHAEELADGLRAILDVPVHLQDERLST
                     VEAERGLAAAGARGPDRRRSVDQAAASLILRAYLDRTAG"
     gene            4248..5300
                     /gene="mltG"
                     /locus_tag="VF058_04650"
     CDS             4248..5300
                     /gene="mltG"
                     /locus_tag="VF058_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX6207632.1"
                     /translation="MLEQAGGAHRARGRALRVLLVLFLVLALLLAGAFGFYVWATGSS
                     GPSRQVTVEIPQGATVTEMGRALEEAGVIRSAFMFGITVRLQGIGAELKAGTYDNMRT
                     NMRLAEALDLLQAGPPPEKLPLSVTVPEGLRIEEVAETLAEGLSIRPRAFLREARSGG
                     WAIEPWLPEGTETVEGFLFPKTYDFPEGVRARAVLRRMLAQFEEEVSGLEWSRAEALG
                     LEPYEVVVMASLIEREARVPKDRRKISAVIHNRLERDMPLEIDATVQYALPEHKERLT
                     FDDLEYESPYNTYLFVGLPPGPIASPGVASIEAALNPADGGWLYYVLIDPETGEHAFA
                     ETYEEFLEYREQAGLG"
     gene            5297..6103
                     /gene="aroE"
                     /locus_tag="VF058_04655"
     CDS             5297..6103
                     /gene="aroE"
                     /locus_tag="VF058_04655"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX6207633.1"
                     /translation="MSTAVFAVVGWPVGHSASPAIHRAAFEALGIPAVYLRLPVPPGR
                     AEAGVGSLRALGARGANVTAPHKVAIAGLCHDLAPSARRTGTVNTLLIEGGTVTGHDT
                     DSEGLRRFLQEDARLNLAGLTSLVLGGGGAARACAVALSGLAGRLVVAARGAAAAAEV
                     ADLFEGPSTVIPFAEAAEAEADVILNATPLGWRGEDVAVDPRPGQVVVDLVYRETPLL
                     RRARAVGAVAHDGRGMLLRQAALSFRLWTSQDPPLEAMRAALDRPLTSAS"
     gene            6160..7005
                     /locus_tag="VF058_04660"
     CDS             6160..7005
                     /locus_tag="VF058_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207634.1"
                     /translation="MPGETIDFEALAVRLSEIDGVRVCLLMSRDGLTLGVHPADREES
                     ARAVWEGMHSIGDPDRGFLTVGDELWIVARRGPYAGIVVAGPSATPGLLLDRLEFSLR
                     AAEELRLREGSATTSSRTEGSRKPRSHLHPEAEPAPLALELDGSSDAVSAAIQIASMR
                     EGPVTVSPQEGNGHRNGSKPPELRLRPLETEATPEPEPEATPEPEPDPEPDPEPDPER
                     GLDIDLDALPEPEVEIEIHPVYDEDEPPRGPGEHFPLPAGDEREVDPVALAREFKNLS
                     DRPDE"
     gene            7050..>7739
                     /locus_tag="VF058_04665"
     CDS             7050..>7739
                     /locus_tag="VF058_04665"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX6207635.1"
                     /translation="MRKTKDLGEVLVDEGLITEDDLGKAHEEQERSGRSLGRVLLDLN
                     LVDETKLVEALAHQIGLQFVDLSEQSVDASAVALVPEQVARRHRAIPIAFEDSTLVVA
                     MSDPSNVIALDDIRTITGFEVRAVVATAADVDAAINKYAKLDQSVEDIAHQAAGAADE
                     DVDLDAMEAAVEEAPIVKLVNLLITQAVADRASDIHVEPAEKDVRIRYRIDGVLHEVM
                     RSPKNIQNGLVS"
BASE COUNT         1081 a         2691 c         2907 g         1060 t
ORIGIN      
        1 cgaggcctcg tctacgaccg cgacgagcac tacgacgtga cctcggcctt catcaagtcg
       61 atgcggggct cggacgccga cgcctcgctg ttctggctgg cccggatgct ggaggcgggg
      121 gaggatcctc gcttcatcgc ccgtcgcatg gtgatcttcg cctccgagga cgtaggcctg
      181 gcggaccccg tcgctctcca ggtggccgtc gccgccgcgc aggccgtgga gttcgtgggg
      241 ctccccgagg tgcgcctcaa cctggcgcac gccgccgtct acctggccag ggctcccaag
      301 tccaacgccg tgatccgcgc gatcgggaag gcggacgagg cccagcggag ggccggccgg
      361 gtgcccacct acctccggga cgcgcactac gccggggcca gggagctggg gcacggcgag
      421 ggctacgcct atcccccgag cgacccggat ggggccgagg gccagcgcca cctcccggaa
      481 ggcgtcgagg gcggcttcct ggacgagtag cgtttcggcc gctccgccct ccacgggtat
      541 gatgtggcga cccgacgaag gagcgacatg tctgcgggtg actgggccct gatcgtcctg
      601 gccgtcttct gggcggtcct cgtcgtcttc ctcgccctcg tgctggtcca gcttgtgcgc
      661 gtgctggaga gcacgaagat cctgatcgac gggatccgga gcgagaccgt ccccctgctc
      721 ggtgaggtca ccacctcggt caagggcgtg aaccgcgagc tcgaccgcgt ggacgacatc
      781 gtcgtctccg ccgccagcat cacgaagtcg gcggagcgtc tcacctcggt cgtcgagcgg
      841 gcgatctcga gcccgctggt gaaggcgatc gcgttcggcg ccggcgcctc cagggccttc
      901 cggcgcctcc gaggatcgtg atgaagcgga tcttctggct ggcgatgggc ctgggggcgg
      961 gggtgacggc cgcggtgatg ctctcccgat ggatgcggcg ccagcaggag cgcctctcgc
     1021 cggccgccat cggcgagcag gtgggcgaga cggcgcgcga cctgggccgt ctgctcaggg
     1081 ccgccctcga cgaggggcgc gaggagatgc gccgggtcga ggccgagatc cgctcctcga
     1141 tcccggagta gcccccgcta gacttgccgc atggaaggcg ccgagatccg tcgccgcttc
     1201 ctgcggcact tcgaggagcg cgatcacacg atcgttccct cgtcgtcgct gatcccgaac
     1261 gacccgacgc tgctgctgac caccgcgggc atggtccagt tcaagccgta cttcctcggg
     1321 gagcaggaac ccccgtatcc tcgcgcggcc agcgcgcaga agtcgttccg cacgaccgac
     1381 atcgagctcg tgggacacac cgatcgccac ctcacgttct tcgagatgct cggcaacttc
     1441 tcgttcggcg actacttcaa ggaggaggcc gtccgcctgg cctgggacct cgtcaccgag
     1501 gggttcggga tcgaccccga ccgcctctgg gccaccgtct accagaccga cgacgaggcc
     1561 gaggagatct ggaaggccta cctcccggcc gaccgtgtcg tgcgccgggg gagggccgac
     1621 aacttctggg acatgggcgt ggccggcccg tgcgggccct gctccgagct ctacgtggat
     1681 cggggtcccg agcacggtgt ggagggcggg cccgaggccg acgaggatcg gtacctcgag
     1741 atctggaacc tcgtcttcat gcagaacgag tgcgacgcgg acatcgaggt cgccggcgag
     1801 ctcccccgga ggaacatcga caccgggatg gggctggagc ggatggccct ggtcctgcag
     1861 gaggccgggt ccgcgttcga gacggacctg ctgcggcccc tcctcgagga ggcggagcgc
     1921 ctcgccgacc gccggtacgg ggccgacgag gacgtggacg tctcgctgcg gatcctggcc
     1981 gagcacgggc gcgccacgac gttcctgatc gccgacgggg tcgtcccgtc gaacgagggg
     2041 cgcgggtacg tcctccgccg gatgctgcgc cgcgtcgtct cccacgcccg ccggctgggc
     2101 atggaaggcg tcgtcctccg cccgctcgta gagcgaaccg tcgagatgct gggcggcgcc
     2161 taccccgagc tcgtggagcg gcgggcgttc gtcctccagg tggcgtcctc ggaggaggag
     2221 cacttcgccc ggacgctcgt ccagggcatg gagctgttcg agcgggaggc cgagcgagcc
     2281 cgcggggccg gggagcgggc tttccccggg gaggcggcgt tcctgtacca cgacacctac
     2341 gggttcccga tcgagctcac gcaggagctg gcccgcgagc acgggctcga ggtggacgag
     2401 gccgcgttcc agcgcctgat ggaggagcag cgggcgcggg cccgccgagc cgcgaagggc
     2461 ggccccgacg atcgggtgct gctggaggtg gcccgcgcgg cggggcgcac cgagttcgtc
     2521 ggctacgcgg aagcggcggc cgaggggaag gtgctggcgc tggtccggaa gggcgagcgc
     2581 gtggaggcgg gcggtgaggg agaggagatc gagctcttcc tcgaccgcac gcccttctac
     2641 gcggaggggg gagggcaggt tggagacgcg ggggtcatcc gcaccgacac gggcgtggtg
     2701 agcgtggccg acaccgttcc cgggcccggc gaggtgatcg tgcaccgggg gaccgtgacc
     2761 gagggcgaga tccgcgcgga ccaggaggct cacgccgaga tcgacctccc caggcgggag
     2821 gcgaccgccc gatcccacac cgggacgcac gtcctccact ggaccctccg gcacctcctg
     2881 ggcgagcacg cgcgccaggc agggtcgctg gtggccccgg ggcgtctccg gttcgacttc
     2941 acccacttcg aggggctctc gcccgatctg ctggaggagc tcgaggccac cgcgaacgcc
     3001 aggctgggcg aggacgccgc ggtgcgagcc tacgagacca ccttcgagta cgcgcgctcc
     3061 cagggggccc tggcgctctt cggcgagcag tacggcgagc tggtccgggt cgtggagatc
     3121 ggcgactact cggtggagct ttgcggcggg acccacgtgg cgcacaccgg cgaggtggcg
     3181 ctcatggtgt tcggctccga gggcagcatc gggtcggggc tgcgccgggt cgaggccctc
     3241 gtcgggcccg acgcgctggc ccacgtgcac gcagagcgac gcctcctgga ggagctcacc
     3301 gagatcctgg gggcccagga tccgcggcag gccgtggagc gggcgaagcg ggccgtggtg
     3361 cgcatcaagg agctggagag ccagctcggc gagctccgcc agcaggagcg gggggaggtc
     3421 gtggactcgc tcctgcggga gacggccgac gtggggggcg tctccctcgt cgtggcgcgc
     3481 ctcgacgagg accccggcac gctgcgggag atggcgctga aactgcggga ccgcctccag
     3541 ggcggccccg gagcggcggt cctggccgcc gccgacggaa ggaaggcgac gctggtggcg
     3601 tcggcgacgc cggagctgat cgatcgaggg gtgacggcgc ccgggctgct ggaggatgcc
     3661 gcccgcgccg tcggaggcgg cgcgggcggc aaggaccacc tcgcgttcgc cggcgggggg
     3721 gcgccggacg ccctcgacga ggcgctccgg ggcctgcccg agcgcctcgt ggcgctcctc
     3781 gggtgatgac ggcgcccggc agggtcctcg ccctcgacgt ggggaaggcc cggatggggg
     3841 tggccgtgtc ggacccggac cgcagggtgg cgctacccgc ggggacgatc cgcgtggtgg
     3901 gcgggatcca ggatctccgt gcggtggccc gcctcgtgga gcggtacgag gcggccgagg
     3961 tggtggtggg gcaccccctc tcgctgtcgg gggagagggg gtcggcggct cggcatgcag
     4021 aggagctggc ggacggcctc cgagcgatcc tggacgtgcc ggtccacctg caggacgagc
     4081 gcctgagcac ggtggaggcc gagcgggggc tggcggccgc cggggcccgg gggcccgacc
     4141 ggcggcgctc cgtggaccag gccgcggcca gcctcatcct gcgcgcctac cttgaccgca
     4201 ccgcgggctg aagggcgcgc gggcgtcgat atcgatatcc tgcggggatg ctcgaacagg
     4261 cggggggagc gcaccgcgca cgcgggcgcg cactgagggt cctgctcgtt ctgttcctcg
     4321 tcctcgccct cctgctggcc ggcgccttcg gcttctacgt ctgggccacc gggagctccg
     4381 ggccctcgcg ccaggtcacc gtcgagatcc cccagggtgc gaccgtcacc gagatgggaa
     4441 gggcgctcga ggaggccggg gtgatccgct ccgcgttcat gttcgggatc acggtcaggc
     4501 tccagggcat cggcgccgag ctcaaggcgg gcacgtacga caacatgcgg accaacatgc
     4561 gcctggccga ggccctggac ctgctgcagg ccggcccgcc cccagagaag ctgccgctct
     4621 cggtgacggt gcccgagggc ctgcggatcg aggaggtggc cgagacgctg gctgagggcc
     4681 tcagcatccg ccccagggct ttcctccgtg aggcgaggag cggcgggtgg gcgatcgagc
     4741 cctggctccc cgaggggacc gagaccgtcg aggggttcct cttccccaag acctacgact
     4801 tcccggaggg cgttcgggcc cgggcggtgc tgcggcggat gctggcgcag ttcgaggagg
     4861 aggtctcggg cctggaatgg tcgcgggccg aggcgctcgg cctcgagccg tacgaggtgg
     4921 tcgtgatggc ctccctgatc gagcgcgagg cccgggtccc caaggaccgc aggaagatct
     4981 ccgcggtgat ccacaaccgc ctggagcggg acatgcccct ggaaatcgac gcgacggtcc
     5041 agtacgcgct gcccgagcac aaggagcggc tgaccttcga cgacctcgag tacgagagcc
     5101 cgtacaacac ctacctgttc gtcggcctgc ctcccggccc gatcgccagc cccggcgtgg
     5161 cctcgatcga ggccgcgctg aaccccgccg acggcggctg gctctactac gtgctgatcg
     5221 accccgagac gggcgagcac gccttcgcgg agacctacga ggagttcctc gagtaccggg
     5281 agcaggccgg cctggggtga gcacggccgt cttcgccgtc gtggggtggc cggtcgggca
     5341 cagcgcctcg ccggcgatcc acagggcggc cttcgaggcg ctggggatcc ccgccgtcta
     5401 cctgcggctc ccggtgcctc ccggccgtgc cgaggcgggg gtcgggtccc ttcgagctct
     5461 gggggctcgg ggggcgaacg tcaccgcccc ccacaaggtc gcgatcgcgg gcctctgtca
     5521 cgacctggct ccctcggccc gcaggacggg caccgtcaac accctcctga tcgagggggg
     5581 aacggtcacg gggcacgaca ccgactccga ggggctgcgg cggttcctgc aggaggacgc
     5641 ccgcctcaac ctcgccgggc tcacctcgct cgtcctgggg ggcggcggcg ccgcgcgcgc
     5701 ctgcgccgtc gcgctgtcgg gtctggccgg gcgcctggtc gtagccgcgc gcggcgccgc
     5761 cgccgcggcc gaggtggccg accttttcga gggcccctcc accgtcatcc cgttcgccga
     5821 ggcggccgag gccgaggccg acgtgatcct caacgcgacc cccctgggat ggcgcggcga
     5881 ggacgtcgcg gtcgaccccc gcccgggcca ggtagtcgtg gacctcgtct accgggagac
     5941 cccgctcctg cggcgggccc gggcggtcgg agccgtcgcg cacgacggcc gggggatgct
     6001 cctgcgccag gcggccctct ccttccgcct gtggacgagc caggaccctc ctctggaggc
     6061 catgcgggcg gcgctcgacc gcccgctcac ctcggcttcc tgacccgcct gcctgcttcc
     6121 ctcaaggtcc cgcacgggac cgccgatgtg atgggtgaca tgcctgggga gactatcgac
     6181 ttcgaggcgt tggccgtgcg cctctccgag atcgacggcg ttcgcgtgtg cctgctgatg
     6241 tcgcgcgacg gcctgacgct cggggtgcac ccggccgacc gggaggagtc cgcccgcgcc
     6301 gtctgggagg ggatgcactc catcggcgac cccgatcgcg gattcctgac cgtgggcgac
     6361 gagctctgga tcgtcgcccg gcgaggtccg tacgcgggga tcgtcgtcgc cggaccctcg
     6421 gcaaccccag gcttgctgct cgaccggctg gagttctcgc ttcgggcggc agaggagctg
     6481 aggctccgag agggttcggc gacgacgtcc tctcgtacgg agggcagccg gaagccccgc
     6541 agccacctcc atcccgaggc cgagccggcg ccgctcgccc tagagctcga cgggtcgagc
     6601 gatgcggtct ccgcggcgat ccagatcgcc agcatgcgtg agggccccgt cacggtctcg
     6661 cctcaggaag ggaacggtca ccggaacggc tccaagccgc ccgagctgag gctccggccg
     6721 ctcgagacgg aggccacccc ggagcccgag ccggaggcca ccccggagcc cgagccggac
     6781 cccgagccgg accccgagcc ggaccccgag cggggtctcg acatcgacct ggacgcgctc
     6841 ccggagcccg aggtcgagat cgagatccac cccgtctacg acgaggatga gcccccccgg
     6901 gggccgggcg agcacttccc cctccccgcc ggagacgagc gggaggtgga tccggtggcc
     6961 ctcgccagag agttcaagaa cctctccgat cgccccgatg agtgaggggg accgatcgag
     7021 agggacgtca tgccgagcga ggggacccga tgaggaagac caaggacctg ggtgaggtcc
     7081 tcgtcgacga gggcctgatc accgaggacg atctgggcaa ggctcacgag gagcaggaac
     7141 ggagcggccg gtcgctgggc cgcgtgctcc tcgatctcaa tctcgtggac gagaccaagc
     7201 tcgtggaggc gctggcgcac cagatcgggc tccagttcgt cgacctctcc gagcaaagcg
     7261 tggatgcctc tgccgtcgcg ctcgtgcccg agcaggtggc gcgccgccac cgggcgatcc
     7321 cgatcgcgtt cgaggactcc accctcgttg tggcgatgtc cgacccgtcg aacgtgatcg
     7381 cgctggacga catccggacg atcaccggct tcgaggtccg ggcggtcgtg gccacggcgg
     7441 ccgacgtgga cgcggccatc aacaagtacg cgaagctcga ccagtcggtc gaggacatcg
     7501 cccatcaagc cgcgggcgcc gccgacgagg acgtggatct cgacgcgatg gaggccgccg
     7561 tcgaggaggc cccgatcgtc aagctcgtca acctcctcat cacccaagca gttgcggacc
     7621 gcgcctcgga catccacgtg gagcccgccg agaaggacgt gcgcatccgc taccggatcg
     7681 acggcgtgct ccacgaggtc atgcgctccc cgaagaacat ccagaacggc ctcgtctcg
//
LOCUS       DASUTW010000142         5406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_172547, whole genome shotgun sequence.
ACCESSION   DASUTW010000142 DASUTW010000000
VERSION     DASUTW010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172547"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VF058_04670"
     CDS             <1..320
                     /locus_tag="VF058_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6207636.1"
                     /translation="GTVDVIISNCVINLSTEKSRVFDEMHRVLRPGGRLGISDIVASD
                     ELIPQQRAERGDFAGCIAGALSIGEYEAGLARAGFEEIEVETTHEVADRMYSAIVRAR
                     RPA"
     gene            562..888
                     /locus_tag="VF058_04675"
     CDS             562..888
                     /locus_tag="VF058_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HEX6207637.1"
                     /translation="MRRLLISLLVLSLALLGALPASAGTRARYGAGAWGDFNGDGYAD
                     LYVGVPFDEVGGNDNVGGVNVIYGGKRGTRPRGNQWWHQDRPRIKGDASRGSRASWRA
                     ATASAC"
     gene            911..1381
                     /locus_tag="VF058_04680"
     CDS             911..1381
                     /locus_tag="VF058_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX6207638.1"
                     /translation="MDADTPALRSLEGSSLRFEVVRTERPSSVEESAALQGIEVEQLL
                     RTIVVRRGADDYVFVLVPGGREIDWPKLRRHLGVRRLSLPGADEAREATGYERGTITP
                     LGSSRAWPVIADGSIPPDRRVAIGGGAHGVNLHLTAGDLIEHLGAEVSDVTKAA"
     gene            1427..2593
                     /locus_tag="VF058_04685"
     CDS             1427..2593
                     /locus_tag="VF058_04685"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HEX6207639.1"
                     /translation="MSQTQDTTPARLGLPEGARGVLPSQALERAIDQGIIDAGDYRVP
                     QKAIQPASVDLRLGETAYRIRSSFLPDKRPVEVKLKEFVVDELDLRRDGAVLETNRPY
                     LIPLIERLDLPPGVRGKANPKSSTGRLDVFTRVITDEGFRFDEIRDGYHGPLYLEVVP
                     LSFTVKVQHKLALSQLRLSIGSARLTDEEIRELHAREPLLFVEGAPAPDEDLATADGL
                     FLGLDLRGDANGRVGYRAKDYAPAIEMARTRAYEWERYWEPVYKEEGDRVVLAPEHFY
                     LLLSEEAVRIPPGYAAEMAAYDPTSGELRTHYAGFFDPGFGFDREGRFHGSRAALEVR
                     AHDVPFMIEHGQRVCKLAFERMAEEPEVLYGEAIGSSYQQQTDTLGKHFIQPPS"
     gene            2857..3138
                     /locus_tag="VF058_04690"
     CDS             2857..3138
                     /locus_tag="VF058_04690"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HEX6207640.1"
                     /translation="MAKALEGLPGYRVIHDLDIGGGNVDHLVIGPTGVFAIETKDWQG
                     RFYAKRGRLHANGRDEDKTRRQAVGGAIWVREQVDRAAEALRHPRPQVP"
     gene            3230..3613
                     /locus_tag="VF058_04695"
     CDS             3230..3613
                     /locus_tag="VF058_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207641.1"
                     /translation="MDERARKRLHDALERLLGSDEAATMMEHLPPVGWADVATRRDVE
                     QLRVATKRDLQELRVGTKRDLQELRVATTRDFQELRVATTRDLDALRLEVQAQMERLA
                     RRVVMWTSSMVVAMTGLSFAAARLL"
     gene            complement(3610..5361)
                     /locus_tag="VF058_04700"
     CDS             complement(3610..5361)
                     /locus_tag="VF058_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX6207642.1"
                     /translation="MAQPLPEKQCDLVMKGGITSGVVYPSAVLRLKDEYRFRSVGGTS
                     VGAIAAAIVAAAEHGRVAGRGTGFAELEAMNEELRQPGLLQSLFVPRRGGRALYEVAS
                     AAMSKRGALHVLRVLLRHGWWALGGLLLWWASLAWAMWFLRGRWWLVPVLLGAWAVAG
                     FVVAAMVTGRWRPAFLLASLVLALGWPALAAPAAVLARGLGELGDQGFGIVSGREEGG
                     TEALGEWLHAHIQRAAGLPDDEPLTFGDLESSEIHLRMMTTDVAAARPVRLPGELQEF
                     SFRPDDVRGILPEPVASRLIERGRELAARRGLPDGDLLPMPHTDEIPILLGFRMSLAF
                     PGLFTAVRLWSEQIQGPPRAHWFSDGGISSNFPIHFFDSWMPTRPTFGLSLGPGTGKA
                     VEPPPKANEARLPGWRRVESLGGFVKAIVDTMQEWRDTMQAELPGFRDRVWEVRLAAD
                     EGGFNIGMGPETIARLIEKGDEAGRSIREEFDWDQHVFTRYLMAMRRLELGLVGGIDP
                     AGREQRGIRAAFDARREWFERPSRVELFDHDAEWLRKAAASTERLLAEMEGWREFGGF
                     AGQSPRPPGEMRVVPDV"
BASE COUNT          833 a         1938 c         1900 g          735 t
ORIGIN      
        1 acggcaccgt ggacgtgatc atctcgaact gcgtgatcaa cctgtcgacc gagaagtccc
       61 gggtcttcga cgagatgcac cgcgtcctcc ggcccggcgg tcggctcggc atcagcgaca
      121 tcgtggcctc cgacgagctc atcccccagc agcgggccga gcgcggggac ttcgccggct
      181 gcatcgccgg cgccctctcg atcggcgagt acgaggcggg gctggcgcgg gccggcttcg
      241 aagagatcga ggtcgagacc acgcacgagg tcgccgaccg catgtacagc gcgatcgtcc
      301 gcgcacgaag gccggcctga cgcgctcagg ccggcaccga gacgtgccga tggtatcctc
      361 gacacggcca gtcggaacgg cccaccaagg ggcccggaaa gccggccggc aacacccaag
      421 gcaaggaacg gctcgtccta gctcggcagg gcgagctttc ttgcatccac agcccttcgg
      481 aggccgccga gcgcggcgac cccttgacct caccccctcc ggtcgatacg gttcgcgcga
      541 ttcgaccagg gaggacctcg catgcgtcgg ctgctcatct ctctcctcgt cctctcgctc
      601 gcccttctcg gagcgctccc cgcgagcgcc gggacccggg ctcggtacgg tgcgggcgcg
      661 tggggcgact tcaacggtga cggctacgcc gacctgtacg tcggcgtccc cttcgacgag
      721 gtcggcggga acgacaacgt cgggggcgtc aacgtcatct acggggggaa gcgcggtacg
      781 cgacccaggg ggaaccagtg gtggcatcag gaccggccgc ggatcaaggg tgacgcgagc
      841 cggggatcaa gggcatcgtg gagagcggcg acggcttcgg cgtgctgacg cgcttcttcg
      901 actgaagggg ttggacgcgg acacgccggc gctgcggagc cttgagggct cgtcgctgcg
      961 cttcgaggtc gtgcgaaccg agcggccgag cagcgtcgag gagagcgcgg cgctccaggg
     1021 catcgaggtg gaacagctcc tccggaccat cgtcgtccgg cgcggagcgg acgactacgt
     1081 gttcgtcctc gtgcctgggg gccgcgagat cgactggccc aagctgcgac ggcacctggg
     1141 ggtgcggcgg ctgtcgctgc cgggcgccga cgaggcccgg gaggccaccg gctacgagcg
     1201 agggacgatc acgccgctgg ggtcgtcgcg ggcgtggccg gtgatcgcgg acgggtcgat
     1261 ccccccggac cggcgcgtgg cgatcggggg cggcgcgcac ggcgtgaacc tccacctcac
     1321 ggcgggcgac ctgatcgagc acctgggagc cgaggtgtcc gacgtgacga aggccgcgtg
     1381 accctggcgg cggtccgcgc ggcgacccta tgatggcccg accgccatga gccagacgca
     1441 ggacaccacc ccggcccgcc taggcctccc cgagggcgcc cgcggcgttc tccccagtca
     1501 ggccctcgag cgcgccatcg accagggcat catcgacgcc ggcgactatc gcgtccccca
     1561 gaaggcgatc cagccggcca gcgtcgacct ccgcctcggc gagaccgcct accggatccg
     1621 ctcgagcttc ctgcccgaca agcgcccggt cgaggtgaag ctcaaggagt tcgtcgtgga
     1681 cgagctcgac ctccgccgcg acggcgccgt cctggagacg aaccgcccct acctcattcc
     1741 cctgatcgag cggctcgacc tgccgccggg cgtgcggggc aaggccaacc ctaagagctc
     1801 caccggccgt ctcgacgtct tcacgcgcgt catcaccgac gaggggttcc gcttcgacga
     1861 gatccgcgac ggctaccacg gccccctcta cctcgaggtc gtgccccttt ccttcaccgt
     1921 caaggtccag cacaagctcg cgctgagcca gcttcggctc tcgatcggca gcgcgaggct
     1981 caccgacgaa gagatccgcg agctccacgc gcgcgagccg ctccttttcg tcgagggcgc
     2041 gccggcgccc gacgaggacc tcgccaccgc ggacgggctc ttcctggggc tcgatctccg
     2101 gggcgacgca aacggccggg tcgggtaccg ggccaaggac tacgcgccgg cgatcgagat
     2161 ggctcgcacc cgggcctacg agtgggagcg gtactgggag cccgtctaca aggaggaggg
     2221 cgaccgggtc gtgctggcgc ccgagcactt ctacctcctg ctgtccgaag aggcggtgcg
     2281 catcccgccg ggctacgcgg ccgagatggc cgcgtacgac cccacgagcg gcgagctgcg
     2341 cacccactac gcggggttct tcgatccggg gttcgggttc gatcgcgaag ggcggttcca
     2401 cggctcgcgc gcggcgctcg aggtgcgggc gcacgacgtg ccgttcatga tcgagcacgg
     2461 ccagcgggtc tgtaagctcg ccttcgagcg gatggccgag gagcccgagg tgctgtacgg
     2521 ggaggcgatc ggctcctcgt atcagcagca gaccgacacg ctcggcaagc acttcatcca
     2581 gcccccgtca tagcgttcaa ggttcggcca gagatacgcc gataggccca gcatgtccga
     2641 tcgtcccggc aggcacctgg agctccgccg ccactccatc gcgaccgtcg tcatcgcagc
     2701 cttcgccgtc ggcctgatgc tgatcgtgat ggggttcttc ttctggccga tggccctgct
     2761 cggcctcctc ctgatcctct acttcgacgg gcagaccaag gacggccggc tcctcgaccc
     2821 aacttcctgg atccggggtc tgcgaggcgg gcgggcgtgg ccaaggcgct cgaggggctc
     2881 cccggctacc gggtcatcca cgacctcgac atcggtgggg ggaacgtgga ccacctcgtg
     2941 atcgggccca cgggcgtctt cgccatcgag acgaaggact ggcagggaag gttctacgcc
     3001 aagcgtggcc gcctccacgc caacggcaga gacgaggata agaccaggcg gcaggcggtg
     3061 gggggagcga tctgggtccg ggagcaagtg gatcgtgccg ccgaagcact gcgccacccc
     3121 cgcccgcagg tcccctgacc caccatctcg tcacaccctc cggctaccat gtccgctacc
     3181 tccctctcga ccgacgttgt cctagagcgg agggaggcgc atgatgtcgg tggatgagcg
     3241 agcgaggaag cggctgcacg acgcgctgga gcgcctcctc gggtccgacg aggcggcaac
     3301 gatgatggag cacctcccac ccgtgggatg ggcggacgtt gccacacggc gggacgtcga
     3361 gcagctgcgt gtggcgacga agcgagacct tcaggagttg cgtgtgggga cgaagcgaga
     3421 ccttcaggag ctgcgtgtgg cgacgactcg ggacttccag gagctgcgtg tggcgacgac
     3481 tcgggacctc gacgcgcttc gcttggaggt ccaggctcag atggagcgcc tcgctcggcg
     3541 cgtcgtcatg tggacctcat cgatggtcgt cgcgatgacg ggtctctcgt tcgccgccgc
     3601 ccgcctcctc tagacgtcgg gaaccacgcg catctcaccc gggggtcgcg gcgactggcc
     3661 cgcgaacccg ccgaactccc tccacccctc catctcggcg agcaggcgct ccgtggacgc
     3721 cgccgccttc cgcagccact cggcgtcgtg gtcgaagagc tcgacccgcg agggccgctc
     3781 gaaccactcg cgacgggcgt cgaacgcggc ccggatcccc cgctgctcgc gccccgcggg
     3841 atcgatccct ccgacgagcc ccagctccag ccggcgcatg gccatcaggt accgcgtgaa
     3901 cacgtgctgg tcccagtcga actcctcgcg gatcgagcgg cccgcctcgt cgcccttctc
     3961 gatgagccgt gcgatcgtct cgggccccat cccgatgttg aacccgcctt cgtcggccgc
     4021 gagccgcacc tcccacacgc ggtcacggaa gcccgggagc tcggcctgca tcgtgtcgcg
     4081 ccactcctgc atcgtgtcga cgatcgcctt cacgaagccc cccagcgact ccacgcggcg
     4141 ccagccgggg agccgcgcct cgttcgcctt cggcggcggc tccacggcct tcccggtccc
     4201 gggtccgagc gacagcccga acgtgggccg ggtgggcatc cacgagtcga agaagtggat
     4261 ggggaagttg ctggagatcc ccccgtccga gaaccagtgc gcgcgcggcg ggccctggat
     4321 ctgctccgac cacagccgca cggccgtgaa cagcccgggg aaggccagcg acatccggaa
     4381 cccgaggagg atggggatct cgtccgtgtg cggcatgggg aggagatcgc catcggggag
     4441 gccgcgtcgg gcggcgagct cgcgcccccg ttcgatcagg cgggacgcga ccggctcggg
     4501 gaggatcccc cgcacatcgt cgggtcggaa cgagaactcc tgcagctcgc cgggcagccg
     4561 cacgggtcgg gccgcggcga cgtcggtggt catcatgcgg aggtggatct ccgagctctc
     4621 caggtcgccg aaggtcaggg gctcgtcgtc ggggaggccg gcggcgcgct ggatgtgggc
     4681 gtgcagccac tcgcccaggg cctcggtgcc gccctcctcc cggccgctga cgatcccgaa
     4741 cccctggtcc ccgagctccc cgaggccccg ggccaacacg gcggcggggg cggccagggc
     4801 cggccacccg agggcgagga cgagcgacgc gaggaggaag gccgggcgcc accgccccgt
     4861 gaccatcgcg gcaaccacga acccggccac ggcccaggct cccagcagga cgggcacgag
     4921 ccaccatcga ccccggagga accacatggc ccaggccagc gaggcccacc agaggaggag
     4981 gcctcccagg gcccaccagc cgtggcggag caggacgcgg aggacgtgga gcgcgccccg
     5041 cttcgacatg gcggcggacg cgacctcgta gagggcgcgt ccgccgcgcc tgggcacgaa
     5101 gagcgactgc agcaggccgg gctggcggag ctcctcgttc atcgcctcga gctcggcgaa
     5161 cccggtcccc cgacccgcga cccgaccgtg ctcggcggcg gccacgatgg cggcggcgat
     5221 cgcgccgacc gaggtgccgc ccaccgaccg gaaccggtac tcgtccttca gccggaggac
     5281 ggccgagggg tagacgacgc cgctggtgat cccgcccttc atgacgagat cgcactgctt
     5341 ctcgggcaga ggctgagcca tggcggacct ccttcgctcg gggcgtgctc agcctggaac
     5401 gggaaa
//
LOCUS       DASUTW010000143        13880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_44349, whole genome shotgun sequence.
ACCESSION   DASUTW010000143 DASUTW010000000
VERSION     DASUTW010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13880
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44349"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VF058_04705"
     CDS             <1..348
                     /locus_tag="VF058_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207643.1"
                     /translation="AAMSLASVVAVLAVSSATGLGAWVLWPGAVAAGAGLFAWNAVVM
                     LAAVTGSEARHAGRASGVVHLGFLSGLALGPVSFGFLVDSTGTYAWSWTGVGVLCLLG
                     ALLARAWVAADND"
     gene            complement(351..1538)
                     /locus_tag="VF058_04710"
     CDS             complement(351..1538)
                     /locus_tag="VF058_04710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX6207644.1"
                     /translation="MRFERAAIPLGAAWSSPFARWQGPFAELSSIDLAASVTTRALAD
                     RGIPAEAMRQLVFGWTVPQPGIFYGAPTLAGRIGAPGISGSMVSQACATSVAAIDVAA
                     MAAEGGRGPNLVVVADRTSNGPHLVYPSPSSRGGTVRSEDWVMDNFACDPWAGESMIA
                     TAEHVATEAGFTRDEIDRVALLRYEQYERALDDDRAFQRRYMVPVEIPQGRGEPVVVE
                     ADAGVYPTTKEGLAKLQPVEPDGVITFGSQTHPADGAAGAVVADESSARELGDGGVVR
                     ILSSGFSRVEKARMPKATVPAAREALADAGLGIEDLDVVMTHNPFAVNDLWFSLETGY
                     PVEDMNPFGCSLVYGHPQGPTGARGVAEVIEALRARGGGVGLFTGCAAGDTGGALVLH
                     VDD"
     gene            complement(1545..3413)
                     /locus_tag="VF058_04715"
     CDS             complement(1545..3413)
                     /locus_tag="VF058_04715"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX6207645.1"
                     /translation="MTSAIDTMDPITFTVLRNGFRSMCSQGSALVERVAWGPVITQGR
                     DYSVGILTADGRLVAHGTVDITPHMGTFEFSVRAVVEDFGDDLAQGDVFIVNDPYRAG
                     THNQDVRLVRPVYLDDELFAFAVACGHWSDMGGPIPGTFNPAAVESWAEGMVIPPTRL
                     YHRDEPVKSTFEMIKMNVRVPHERMGDAAAQHQATKLIERRLGEYVERYGKETVAQGF
                     EAVMDYAERLFRAEVADLPDGTYEFTDAADHDIGTPGHPRVTFRCKLVIENDRVTVDW
                     TESDDAPKGPTGLTLPALSSATYDGTLHCFPHLVPLNHGIIRALDIKTRPGSAAHVLH
                     PTPVAGYCSSGYEKTDHAMMGAWGQAMAAAGRPEMIFAGTVNLQNCVIGGIHPKTGQR
                     YVSYTWSEGGQGARSYADGPSFAMFLYGAGAQNQPIEVHERWYPFLYEKFEVAVDSCG
                     DGRYRGGYGSHRRWRMLGDAVNSIHGDRAEVTPPGVAGGTNGGPNLLIQNMGTPEEND
                     LGMFATNVPLPKDSTIDFISNGGGGFGNPLDREPEVILEEVIDGFLSIEKAKEVYGVV
                     IDAVDPDVHDFRIDEKATEELRLSLRDRTPPVGTGPGEAHPDGLRTGELLAQSAGA"
     gene            complement(3427..5493)
                     /locus_tag="VF058_04720"
     CDS             complement(3427..5493)
                     /locus_tag="VF058_04720"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX6207646.1"
                     /translation="MSRYIVAVDIGGTFSDLVSLDRDTGEIRHAKVPSTPPTFIDGVV
                     NALEKVAIPASDMAVFKHGSTIATNAIIERKGARTALVTTEGFSDVLGAARADRTDHF
                     DLSAIERPPLVPRRDVLGVRERVDYEGSPVVPLDEESLRRAVELIEKRDIEAVAVVFL
                     NSFMNPEHEYRTVQAIQEALPDVYVCCSYDILPELKEFERTSTTTVNAYIGPIVDRYL
                     SNLTKRLADWGFRNDTFIIHSGGGVMSLDAARRLPSRTCMSGPAGGVVGAAYIGGLAG
                     FPGVLSFDMGGTSCDVGLISDGKPGHAAGWKIEHKVPVQFPAVDVSTIGAGGGTIAWI
                     DRAGSLRSGPQSAGANPGPAAYDTGGTEPTNTDANLVLGRLNPGTFLGGDLPLKPELA
                     EQAIRTTIAEPLGLSLEEAAEGILRLSNTNMVNAARLVTVERGHDPRDYALMAFGGAG
                     PVHAAYCASELAIPTVLVPLSPGIASAFGQLRVEIRDDYQRPLLKKHGDVTPTLLEKV
                     FAEMEEQAKESLTREGISADEMRLERTVDLKYYPQTTYLNFEVPDGPIDEGTIDALVE
                     RFLERHEREFGYSVPLEFTSVEFVNARLTALGPAPIGELKRTTETGKAEDAVSGTRQV
                     HFSEAGGWVETNIYDRSLLRAGASFDGPAIVEEADSTTVVPPGARVEVDEYLNMIVDV
                     REMPRT"
     gene            complement(5490..6839)
                     /locus_tag="VF058_04725"
     CDS             complement(5490..6839)
                     /locus_tag="VF058_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6207647.1"
                     /translation="MPDGTYWNPEIETMPWLEVEAMQGELLQQQLAYVHERSPFYESK
                     WKEAGFDVTKVRSLDDLKEAPFTTKQELRDSQIASPPLGEHAAVDLVDVIRIHSSSGT
                     TGRPSYVGVTRADHQVWTETISRVYYCEGVRPEDVLIHGFGLGFFVGGLPLAHAVENI
                     GATFVPIGTGASDRLVTSIQNLGGTILTCTPSYANYLAEHLREKFEMEPSELGLRRIL
                     LGAEPGGGIPAVRQRLADDYGALVTEGLGNADLIPVYGANCDELDGNHFLGPDFMALE
                     VVDPETGESLPLEDGVEGELLATHLRRECVPLVRFRVGDRVVVKSSPCACGRTAPRMT
                     CVGRVDDMLIVAGVNVWPSAVKDVITDLYPRTTGAMEILLEAEPPRVEPPLRIQAEYG
                     PEATDLDALKKEIEGILREKLIVKTDVELVPPDTLPRFEMKAKLIRKLYEEGEVTRA"
     gene            complement(6832..7533)
                     /locus_tag="VF058_04730"
     CDS             complement(6832..7533)
                     /locus_tag="VF058_04730"
                     /inference="COORDINATES: protein motif:HMM:NF019456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX6207648.1"
                     /translation="MTDEQHRFRSIGSKRSFELVIREMQKAITRGDLKPGQKLPAEPD
                     LAAQFGVSRSALREALKALELSGYLEVRRGYGGGTFVSAPEFEEFTTVAPPPISSLDI
                     TRRHLLEVRLVIEPQAARLAVKAPFEEILSLHEAIRHMETFDDRPAHVLASAANFHIG
                     LARASGNPVFVSVLEGLRPVMYRAMSKLVTDSEWREGCRLQHETIVKEIEKGDADVAE
                     RAMRTHLSMKEGEGA"
     gene            7715..9376
                     /locus_tag="VF058_04735"
     CDS             7715..9376
                     /locus_tag="VF058_04735"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX6207649.1"
                     /translation="MGVLDPDLALLNGVVYTVDDDFRTAEAIAVKGETIVATGSTDEV
                     RSLAADDTRVIDLEGRAVVPGFMDSHLHPVYAGKNLSQVQFADCRTVADMLAVIKERA
                     DALPAGEWFRGSNHWTPDQLEERRLPYRDELDGVAPENPFWGNTAFHRAMANSLALQA
                     CGIDRDTPERFAGGSGYVFKDPETGEPNGHLLETAMFCVMQQEPPESVEEVLEGIDQV
                     QNLFLRNGITSVIDQGDVGPPFRNFQLMQRLHRQGNLKLRWRMNHIGFEMADMPLEAI
                     PDHVASLRVLSGFGDDWLRMGAIGELVLDGFVEDNYSREPYAEDVFGEGWRGILLYRP
                     DTVMAICRAAAANGLQMNVHVQGDGALDLALDTFEGIHRESSIAGLNWALEHGALTPT
                     DKNLRQIQQMGIVLSTQQPLYYWHSHDAIKFLGPAAEDFFPNRTWHENGVSLRGGSDF
                     DTAPLSPLFGIWTIVTRRNILGEVVGPSQTLPREEALRMYTRNGAASVFEDMRKGSIE
                     AGKLADLVVLSDDLMAVPEDGIKDIKVVATIVGGGTSYDPEGLLA"
     gene            complement(9383..10921)
                     /locus_tag="VF058_04740"
     CDS             complement(9383..10921)
                     /locus_tag="VF058_04740"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX6207650.1"
                     /translation="MVATSHPLGVDAGLGAIEAGGNAVDAAVAAAAVLAVVDPPSTGI
                     GGDLFALVWPVGSPSPQALAAAGCAPAGLSVDALRDAGYGSMPGDGAWTVTVPGGVAG
                     WAELLERQGRLGLDAALRPAIAAAEGGFQVTPVVARSWAEEASRLARNEASRAAFLLA
                     GRAPAPAERFANPDLARTLRAIAEEGPAVLYGGPLGERIAAAVERAGGPMRASDLAAW
                     GGPSWSEPIAIDHGGARVYEHPPPGQGIVVLEALGIYEKLDTSGRPQDEHAAIEALRL
                     AFADAFQHLADPAKVALPIDQLLAKSRLQRLARDVRPHEAARLLVGDAPGLDTVYVAA
                     VDPEGGGCSLIQSLFHRFGSKLTVPETGVVLQNRGAGFTLTEGHPNRPEPGKRPYHTI
                     IPGMLGATGRFLACLGVVGGFMQPQGQLQIIRNVLDRDMDPQAAIDAPRFRVAERAET
                     SRVGLEKDFPSEVADELAARGHAVVALPWWEAGGAQMILRDEDGSLVGGSDPRKDGLA
                     RALE"
     gene            11056..11859
                     /locus_tag="VF058_04745"
     CDS             11056..11859
                     /locus_tag="VF058_04745"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6207651.1"
                     /translation="MDEQGPATDTAGSATGGGPTVRARGITKVFPRGKVVALEDVSFV
                     IKSGQFTSVVGPSGCGKSTLLRLVAGLIPRSYGELDVHGSDVTGPRTDIAMMFQRPIL
                     LPWKTALENVVLPRKIRGRLDERDWREGRSLLRLLALEEFRHAYPQHLSGGMQQRVAL
                     ARLLITGADLLLMDEPFASLDEFTRERLNLELLRIQSEVAATVMFVTHNITEAVFLAD
                     QVLVMTPRPGRLAGIVEVPFGRPRTIELLRSPEFNDLAFKVRGLLGESI"
     gene            11856..12740
                     /locus_tag="VF058_04750"
     CDS             11856..12740
                     /locus_tag="VF058_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6207652.1"
                     /translation="MKTREDPDVAAVEAQRLTELETSSLARRVGSAWPAFLVFALLVA
                     AWEGAARAELISRLVLPAPSRIFLALIELFTSGLIWEHLRATFLETMAGFAVGAGSAF
                     VLAVLSSFWGWFRQIVSPYMIILQVTPRVALAPIFITWFGFGLMPKIVMAATICFFPV
                     FINTLTGLLNTDEEALEMFRSMGASKRQIFTQFTMPSAMPVTFAGLKTGITLALIGAI
                     VAEFVATSQGLGLLIDRFNFRLAMEEAFAVLLVLALLGLLLYGVMEFLDRRIVFWTRE
                     ERLSKREIRSRRGREDTA"
     gene            12737..13810
                     /locus_tag="VF058_04755"
     CDS             12737..13810
                     /locus_tag="VF058_04755"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6207653.1"
                     /translation="MKRARFIALLAAAVLVASACGGDDGGGGGGDGEVVEIDFLQPLP
                     KSIAFYPLFVGERLGYFEEEGVRVNLLPSGDIPATVAVPAGNADIGATTPANIVVGAS
                     EEEDFVLVYEYYQKNVFSIVVPENSDVQEVADLAGRNVGITSEGAGDAALTRAALTQA
                     GLDPDADVTITVVGEGGPTVANALDEGSIDAFTGAINDIVALRVAGVEVRNITPENLD
                     ILPASSMIAQPSQIQEMGDAMQGFLRAWAKATYAGMHNPDAVYAMAQEEVPEETGDEE
                     FGRIFLEQAIDLQTPVEGTDNFGALRPDAWSAVMDQLIEGGELEETFDPSSFLDDRFI
                     EGANDFDRAEVEQEIDDWFSENG"
BASE COUNT         2100 a         4822 c         4736 g         2222 t
ORIGIN      
        1 gcggccatga gcctcgcctc ggtcgtggcc gtgctcgcgg tctcctccgc gacgggcctg
       61 ggtgcgtggg tcctgtggcc cggggcggtc gcagcggggg ccgggctctt cgcctggaac
      121 gccgtcgtga tgctggccgc ggtcactgga tcggaggctc ggcacgccgg ccgagcatcg
      181 ggggtggtcc atctcgggtt cctgtcggga ttggccctgg ggcccgtgag cttcgggttc
      241 ctcgtggact cgaccggcac gtacgcttgg tcctggaccg gcgtgggcgt cctctgcctc
      301 ctcggcgcgc tcctcgcaag ggcctgggtg gcggcggaca acgactgagt ttagtcgtcc
      361 acgtggagga ccagagctcc cccggtgtct cccgccgcgc atcccgtgaa aagcccgacg
      421 cccccgcccc tggcccgcag ggcctcgatc acctcggcca cgccccgcgc cccggtcggg
      481 ccctgcgggt gcccgtagac gaggctgcac ccgaacgggt tcatgtcctc aaccgggtac
      541 cccgtctcca gggagaacca gaggtcgttc acggcgaagg ggttgtgggt catgacgacg
      601 tcgagatcct cgatcccgag accggcgtcc gcgagcgcct cacgtgccgc cgggaccgtg
      661 gccttgggca tcctcgcctt ctcgacgcgg gagaagcccg agctcaggat gcggacgacg
      721 ccgccatcgc cgagctcccg agcgctcgac tcgtccgcga ccaccgcgcc tgccgcgccg
      781 tcggcgggat gggtctggct cccgaaggtg atcacgccgt ccggctcgac gggctggagc
      841 ttggcgagcc cctccttcgt ggtgggatag acgccggcgt ccgcctccac cacgacgggc
      901 tctcccctcc cctgcgggat ctcgacgggg accatgtacc ggcgctggaa cgcgcggtcg
      961 tcgtcgaggg cccgctcgta ctgctcgtac cggaggaggg ccacccggtc gatctcgtcc
     1021 cgggtgaagc cggcctccgt cgcgacgtgc tcggccgtgg cgatcatcga ttcgccggcc
     1081 cacgggtcgc acgcgaaatt gtccatcacc cagtcctcgg accgaacggt gcccccccgg
     1141 ctggagggcg acggatacac gaggtgcgga ccgttgctcg tgcggtcggc gacgacgacg
     1201 aggttcggcc cacgcccgcc ctccgcggcc atcgcagcga cgtcgatcgc cgcgaccgag
     1261 gtggcgcagg cctgggaaac catcgagccc gagatccccg gcgccccgat cctcccggcc
     1321 agcgtcggag cgccgtagaa gatgcccggc tgcgggaccg tccagccgaa cacgagctga
     1381 cgcatcgcct ccgccgggat accgcggtcg gccagtgccc tcgtggtcac gcttgcggcg
     1441 aggtcgatgc tggacagctc ggcgaacggt ccctgccagc gggcgaacgg cgaggaccac
     1501 gccgccccga gagggatcgc cgcccgctcg aacctcaccg ctcctcaggc tcccgcgctc
     1561 tgggccagca gctcgccggt ccgaagcccg tcgggatggg cttccccggg gcccgtcccc
     1621 acggggggcg tccggtcacg cagcgagagc cgcagctcct ccgtggcctt ctcgtcgatc
     1681 cggaagtcgt gcacgtcggg gtccaccgca tcgatcacga cgccgtagac ctccttcgcc
     1741 ttctcgatcg agaggaagcc gtcgatcacc tcctccagga tcacctcggg ctcgcggtcc
     1801 agcgggttcc cgaagccgcc cccgccgttg gagatgaaat cgatcgtgct gtccttgggc
     1861 agggggacgt tcgtggcgaa catcccgagg tcgttctcct cgggcgtccc catgttctgg
     1921 atcagcagat tgggcccgcc gttcgtcccg cccgccacgc cgggtggggt gacctcggcc
     1981 cgatctccgt ggatcgagtt gaccgcgtcg cccagcatcc gccagcggcg gtgcgacccg
     2041 tagccacccc ggtaccgacc gtccccacac gagtccaccg cgacctcgaa cttctcgtag
     2101 aggaacgggt accaccgctc gtgcacctcg atcggctggt tctgagcgcc ggccccgtac
     2161 aggaacatgg cgaagctggg gccgtcggcg tagctgcgcg ccccctggcc gccctcggac
     2221 cacgtgtagg agacgtaccg ctggccggtc ttcggatgga tgcctccgat cacgcagttc
     2281 tggaggttga ccgtgccggc gaagatcatc tcgggccggc ccgccgccgc catggcctgc
     2341 ccccatgctc ccatcatcgc gtggtctgtc ttctcgtacc ccgaggagca gtacccggcc
     2401 acgggcgtgg gatgaagcac gtgcgccgcc gacccgggcc tcgtcttgat gtcgagggcc
     2461 cggatgatcc cgtggttcag cgggacgagg tgcgggaagc agtgcagggt cccgtcgtaa
     2521 gtggcgctgg agagcgccgg cagggtgagc ccggtcgggc ccttcggggc gtcgtccgac
     2581 tcggtccagt ccacggtaac ccggtcgttc tcgatcacga gcttgcagcg gaacgtcacc
     2641 cgcggatggc cgggcgtgcc gatgtcgtgg tcggccgcgt ccgtgaactc gtacgtgccg
     2701 tccggcagat cggccacctc ggcacggaag agcctctccg cgtagtccat caccgcctcg
     2761 aagccctggg cgaccgtctc cttcccgtac cgctcgacgt actcgccgag ccgccgctcg
     2821 atcagcttcg tggcctggtg ctgcgccgcc gcgtcgccca tccgctcgtg ggggacgcgg
     2881 acgttcatct tgatcatctc gaaggtcgac ttcaccggct cgtcccggtg gtacaggcgc
     2941 gtgggcggga tcaccatgcc ctccgcccac gactcgacgg cggcggggtt gaaggtgccc
     3001 gggatcggac cgcccatgtc ggaccagtgc ccgcaggcca cggcgaacgc gaacagctcg
     3061 tcatcgaggt acacggggcg gacgagccgc acgtcctggt tgtgggtccc cgcccggtag
     3121 gggtcgttga cgatgaagac gtcgccctgc gccaggtcgt cgccgaagtc ctccaccacc
     3181 gcgcgcaccg agaactcgaa cgtccccatg tgcggcgtaa tgtccacggt gccgtgcgcc
     3241 accagccgac cgtccgcggt gaggatgccc accgagtagt ctcggccctg cgtgatcacc
     3301 gggccccagg ccacccgctc gacgagggcc gagccctgag agcacatgga gcggaacccg
     3361 ttccgaagca ccgtgaaggt gatcgggtcc atcgtgtcga tcgctgacgt catcggtctc
     3421 cttccctcac gtccgcggca tctcgcggac gtccacgatc atgttcaggt actcatccac
     3481 ctcgaccctg gccccgggcg ggaccaccgt ggtcgagtcg gcctcctcca cgatggccgg
     3541 gccgtcgaag gacgcgcccg cccgcaggag gctccggtcg tagatgttcg tctccaccca
     3601 gccgccggcc tcggagaagt ggacctgccg cgtgcccgag acggcgtcct cggccttccc
     3661 cgtctccgtc gtgcgcttca gctcgccgat cggagcgggg ccgagggcgg tgagcctggc
     3721 gttgacgaac tcgacgctcg tgaactccag cggcaccgag tagccgaact ctcgctcgtg
     3781 ccgctcgagg aacctctcga cgagggcgtc gatcgtcccc tcgtcgatcg ggccgtccgg
     3841 gacctcgaag ttgaggtacg tcgtctgcgg gtagtacttg aggtccacgg tccgctcgag
     3901 ccgcatctcg tcggccgaga tcccctcgcg cgtgaggctc tccttcgcct gctcctccat
     3961 ctcggcgaag accttctcca gcagcgtcgg cgtgacgtct ccgtgcttct tgaggagcgg
     4021 acgctggtag tcgtcgcgga tctccacgcg gagctggccg aaggcgctgg cgatgcccgg
     4081 gctgagcgga acgagcacgg tcgggatcgc gagctcgctc gcgcagtacg cggcgtgcac
     4141 cggcccggcg cccccgaacg ccatcagcgc gtagtccctc gggtcgtggc cgcgctcgac
     4201 cgtgacgagt cgcgccgcgt tgaccatgtt cgtgttggag aggcggagga tgccctcggc
     4261 cgcctcctcg agcgacagtc ccagcggctc ggcgatcgtc gtgcggatcg cctgctcggc
     4321 gagctccggc ttcaggggga gatccccacc gaggaaggtg ccggggttca gccgcccgag
     4381 gacgaggttg gcgtccgtgt tcgtgggctc ggtgccgccg gtgtcgtagg ccgcggggcc
     4441 cgggttggct ccggcgctct gcggaccgga gcggaggctg cccgcgcggt cgatccaggc
     4501 gatcgtgccc ccgcccgccc cgatcgtgga aacgtccacg gccggaaact gcacggggac
     4561 cttgtgctca atcttccacc cggcggcgtg gccgggcttc ccgtcgctga tgagccccac
     4621 gtcgcacgag gtcccaccca tatcgaagct gagcacgccc gggaagccgg cgaggccccc
     4681 gatgtacgcg gcgccgacga cgccgcccgc cggccccgac atgcaggtgc gtgacgggag
     4741 ccggcgggcg gcatcgaggc tcatcacgcc gccccccgag tggatgatga acgtgtcgtt
     4801 ccggaatccc cagtccgcga ggcgcttcgt gaggttggag aggtatcgat cgacaatggg
     4861 accgatgtag gcgttgaccg tcgtggtgga ggtacgctcg aactccttca gctccggcag
     4921 gatgtcgtag gagcagcaca cgtaaacgtc cggcagggcc tcctggatcg cctgcacggt
     4981 ccggtactca tgctcggggt tcatgaagga gttgaggaag acgacggcga cggcttcgat
     5041 atcccgcttc tcgatgagct cgacggcgcg gcggaggctc tcctcgtcga gagggacgac
     5101 cgggctcccc tcgtagtcca cacgctcgcg gacgccgagg acgtcccgcc tcggcaccag
     5161 cggcgggcgc tcgatcgccg acagatcgaa gtggtcggtc cggtcggcac gcgcggctcc
     5221 gagcacatcg ctgaacccct cggtggtgac gagcgccgtg cgggcgccct tccgctcgat
     5281 gatcgcgttg gtcgcgatcg tggaaccgtg cttgaacacg gccatgtcgg atgccgggat
     5341 ggcgaccttc tccagagcgt tgaccacccc gtcgatgaag gtgggcgggg tcgaggggac
     5401 cttggcgtgc cggatctccc ccgtgtcgcg gtcgagcgag acgaggtctg agaacgtccc
     5461 cccgatgtcg accgcaacga tgtacctgct catgcgcgcg tcacctcgcc ttcctcgtag
     5521 agcttgcgga tcagcttggc cttcatctcg aagcggggaa gcgtgtccgg ggggaccagc
     5581 tccacgtcgg tcttcacgat cagcttctcg cgaaggatcc cctcgatctc cttcttcagc
     5641 gcgtcgaggt cggtcgcctc gggcccgtac tcggcctgga tccgcagcgg cggctcgacg
     5701 cggggcggct ctgcctcgag caggatctcc atggcgccgg tcgttctcgg gtacaggtcc
     5761 gtgatcacgt ccttcaccgc ggagggccag acgttgaccc cggcgacgat cagcatgtcg
     5821 tccacccgcc cgacgcacgt catgcggggc gcggtccgtc cgcaggcgca gggggaggac
     5881 ttcacgacga cccgatcgcc gacgcggaag cgcacgagcg ggacgcactc gcgcctgaga
     5941 tgcgtggcga ggagctcgcc ctcgaccccg tcctcgaggg ggagcgactc ccccgtctcc
     6001 gggtcgacga cctcgagcgc catgaagtcg ggtcccagga aatggttgcc gtcgagctcg
     6061 tcgcagttgg ccccgtacac ggggatcagg tcggcgttcc cgaggccctc ggtgaccaag
     6121 gcgccgtagt cgtcggcgag ccgctggcga acagccggga tccccccgcc gggctccgcg
     6181 cccaggagga tccgcctcag ccccagctcg gagggctcca tctcgaactt ctcgcggagg
     6241 tgttcggcga gatagttggc gtaggagggg gtgcacgtga ggatcgtccc gccgaggttc
     6301 tggatgctcg tgaccaggcg gtcggacgcg ccggttccga tcgggacgaa ggtcgccccg
     6361 atgttctcaa cggcgtgcgc gagcggcagg cccccgacga agaacccgag cccgaaaccg
     6421 tggatcagga cgtcctctgg acggacgccc tcgcagtagt agacgcgact aatggtctcg
     6481 gtccagacct gatggtcggc ccgggtgact ccgacgtagc tggggcggcc cgtggtcccg
     6541 cttgaggagt ggatccggat cacgtccacg aggtcgaccg cggcgtgttc cccgagcggg
     6601 ggggatgcga tctggctgtc gcgcagctcc tgcttcgtgg tgaagggcgc ctccttgaga
     6661 tcgtcgagcg accgcacctt cgtcacgtcg aaccccgctt ccttccactt cgactcgtag
     6721 aagggagatc gctcgtggac gtaggccagc tgctgctgca ggagctcgcc ctgcatcgcc
     6781 tcgacctcga gccacggcat cgtctcgatc tcggggttcc agtaggttcc gtcaggcacc
     6841 ttcgccctcc ttcatggaca ggtgggtccg catcgcgcgc tccgccacgt cggcgtcacc
     6901 cttctcgatc tccttgacga tcgtctcgtg ctgcaggcgg cagccctccc gccactcgga
     6961 gtcggtgacg agcttcgaca tggcccggta catcacgggg cggagcccct ccaggaccga
     7021 gacgaagacg gggttccccg acgccctggc caggccgatg tggaagttgg ccgcggaagc
     7081 caagacgtgc gcgggacggt cgtcgaaggt ctccatgtgg cggatggcct cgtgcaggct
     7141 caggatctcc tcgaaggggg ccttgacggc gaggcgcgcc gcctggggct cgatcaccag
     7201 ccgaacctcg agcagatggc ggcgcgtgat gtcgaggctg gagatgggag gtggagccac
     7261 cgtggtgaac tcctcgaact cgggcgccga aacgaaggtc ccgccgccgt accctctgcg
     7321 gacctcgagg tagccggaga gctccagggc cttgagggcc tcgcggagcg cggaacggga
     7381 gacgccgaac tgcgcggcca ggtccggctc ggcgggcagc ttctgtcccg gcttcaggtc
     7441 cccccgcgtg atcgccttct gcatctcgcg gatgacgagc tcgaagctcc tcttggagcc
     7501 gatggatcgg aatcgatgct gctcgtcggt caaagcggtc gacctcccat cgccggtatg
     7561 atgtcggacc gcacaggagg ttcgcaaccc tgaggacgcg gccccgccgc gatctctctc
     7621 cgattcaacg ccgctcacct ccccgagttg cgttccgccc ccgaccccag gactatagtt
     7681 cgaagtccga catcagtcga aaggggtgtg gcccatgggg gtcctggatc ccgacctcgc
     7741 gctcctgaac ggggtcgtct acacggtgga cgacgacttc cgaaccgccg aggcgatcgc
     7801 cgtcaagggg gagacgatcg tcgccaccgg ctccaccgac gaggtccgaa gcctcgcagc
     7861 cgacgacacc cgcgtcatcg acctcgaggg gagggccgtc gtccccggct tcatggactc
     7921 gcacctccat cccgtgtacg cgggcaagaa tctctcccag gtgcagttcg ccgactgccg
     7981 gacggtcgcc gacatgctgg cagtgatcaa ggagcgggcc gacgcgcttc ccgcgggcga
     8041 gtggttccgc gggtcgaatc actggacccc cgaccagctc gaggagcgac ggctcccgta
     8101 tcgggacgag ctggacgggg tggctccgga gaacccgttc tggggcaaca cggcgttcca
     8161 tcgcgccatg gcgaacagcc tggcccttca ggcgtgcggg atcgaccggg acacccccga
     8221 gcggttcgcg ggagggagcg gttacgtctt caaggatccg gagaccggcg agcccaacgg
     8281 ccacctcctg gagaccgcga tgttctgcgt catgcagcag gagccgcccg agagcgtcga
     8341 ggaggtcctc gagggaatcg accaggtgca gaacctcttc ctccggaacg ggatcaccag
     8401 cgtgatcgat cagggcgacg tggggccgcc cttccgaaac ttccagctga tgcaacggct
     8461 ccaccgccag gggaatctca agctccgctg gcggatgaat cacatcgggt tcgagatggc
     8521 ggacatgccg ttggaggcga tccccgacca cgtggccagc ctgcgggtcc tctcggggtt
     8581 cggggacgac tggctccgga tgggcgccat cggcgagctc gtcctggacg gcttcgtcga
     8641 ggacaactac tcgcgcgagc cgtacgccga ggacgtcttc ggcgaaggat ggcgaggtat
     8701 cctcctctac cgtccggaca cggtgatggc gatctgtagg gcggccgccg cgaacggtct
     8761 ccagatgaac gttcatgtgc agggcgacgg ggccctcgat ctggccctcg acacgttcga
     8821 ggggatccat cgggaatcgt caatcgcggg actgaactgg gcgctcgagc acggcgccct
     8881 cacgcccacg gacaagaacc tgcgccagat ccaacagatg gggatcgtgc tctccaccca
     8941 acagccgctc tactactggc actcgcacga cgccatcaag ttcctcgggc cggcggcgga
     9001 ggacttcttc cccaaccgaa cgtggcacga gaacggcgtc tcgctgcgtg gcggctccga
     9061 cttcgacacc gctccgctgt caccgctctt cgggatctgg acgatcgtca cccggcgcaa
     9121 catccttgga gaggtcgtgg gcccgagcca aacgctccct cgtgaggagg ccctgaggat
     9181 gtacacgcgc aacggagcgg cctccgtgtt cgaggacatg aggaagggct ccatcgaggc
     9241 gggcaagctc gccgatctcg tcgtcctctc cgacgacctc atggccgtcc ccgaggacgg
     9301 catcaaggac atcaaggtcg tcgcgacgat agttgggggt gggacgtcct acgatccgga
     9361 agggctgctc gcctgaaggt cctcactcga gtgcacgggc gagcccgtcc ttccgagggt
     9421 ccgagccccc cacgagcgac ccgtcctcgt cgcggaggat catctgggcg ccccccgcct
     9481 cccaccatgg aagggcgacc accgcgtgtc ccctcgccgc tagctcgtcg gccacctcgc
     9541 tggggaagtc cttctccagg ccaacccgcg aggtctccgc ccgctcggcg actcggaacc
     9601 tcggcgcgtc gatcgcggct tgcgggtcca tatctcgatc gaggacgttc cggatgatct
     9661 gcagctgccc ttgaggctgc atgaaccctc cgaccactcc gagacacgcg aggaagcggc
     9721 ccgttgcgcc gagcatcccg gggatgatcg tgtggtacgg acgcttcccc ggctccgggc
     9781 ggttcgggtg cccctccgtg agcgtgaacc cagcgccacg attctggagg acgaccccgg
     9841 tctccgggac cgtcagcttc gatccgaacc gatggaagag gctctgaatc aacgagcaac
     9901 ccccgccctc gggatccacg gcggcgacgt acacggtgtc aagcccgggg gcatctccca
     9961 cgagcagtcg cgccgcctcg tggggacgca cgtcgcgcgc gaggcgttgg agccgtgatt
    10021 tggcaaggag ctgatcgatc gggagggcga ccttggcggg gtcggcgaga tgctggaacg
    10081 catccgcgaa cgccaaccgc agcgcctcga tcgccgcgtg ctcgtcctgg ggacggcccg
    10141 acgtgtcaag cttctcgtag attccgagcg cctcgagcac cacgatgccc tggccaggag
    10201 ggggatgctc gtacaccctg gcccctccgt gatcgatcgc aatcggctcg gaccaggatg
    10261 gcccccccca cgcggcgagg tcggaggctc gcatgggccc accggccctc tccaccgcgg
    10321 ccgcgatccg ctcccccagg ggaccaccgt agaggactgc tgggccctct tccgcgattg
    10381 ccctcagggt tcgggcaaga tcggggttgg cgaagcgctc ggcgggcgcg ggcgccctcc
    10441 ccgccaggag gaacgcggcc ctggatgctt cgttacgagc gagcctcgag gcctcctcgg
    10501 cccagctccg ggcgaccacc ggggtgacct ggaacccacc ttcggccgcg gcgatcgcgg
    10561 ggcgaagggc cgcgtcgagg cccagccgcc cctgccgctc gagcagctcc gcccatccgg
    10621 ccacaccgcc ggggacggtg acggtccagg ccccgtcgcc gggcatcgac ccgtacccgg
    10681 catcgcgtag ggcgtcgacc gagaggcccg cgggagcgca gccggcggcg gcgagggcct
    10741 ggggcgaggg actaccgaca ggccagacga gcgcgaacag gtcacccccg atccccgtgg
    10801 aggggggatc cacaacggcg aggaccgctg ccgctgccac cgcggcgtcc acagcgttcc
    10861 cgccggcctc tatggccccc agccccgcgt ccacgcccag ggggtgactc gtggccacca
    10921 tcccgcccct cgagcgcacc gacctgcggc ccgatgcccc gtcttgcgcc cgcgccacca
    10981 tcggtgctaa gactacctga catctgaagt cggacttcag cctgatggct ccgtgagggg
    11041 ggaacgccca gggtgatgga cgagcagggc ccggccacgg acaccgcagg gtcggcgaca
    11101 gggggtggcc cgaccgttcg ggcccggggg atcacgaagg tgttccctcg cgggaaggtg
    11161 gtggcgctcg aggacgtctc cttcgtgatc aagagcggcc agttcacttc cgtcgtcgga
    11221 cccagcggct gcggcaagtc cacactgctg cgcctcgtgg ccgggctcat cccaaggtcg
    11281 tacggggagc tggacgtcca cggctccgat gtgactggtc cccgcaccga catcgcgatg
    11341 atgttccagc gtccgatcct cctgccctgg aagacggctc tggagaacgt cgtgctcccc
    11401 cgcaagatac gaggtcgcct cgacgagcgg gattggcggg agggaaggag ccttctccgc
    11461 ttgctcgcac tcgaggagtt ccgccatgcc tacccgcagc atctctccgg agggatgcag
    11521 cagcgtgtgg ccctggcgcg gctgcttatc accggcgcgg acctcctcct gatggacgag
    11581 cctttcgcct ctctcgacga gttcacccgc gagcggctga acctggagct gctccggatc
    11641 cagtcggagg tggcagcgac cgtcatgttc gtcacccaca acatcacgga agctgtcttc
    11701 ctcgccgacc aggtgcttgt gatgacccct cggccggggc gactcgccgg catcgtagag
    11761 gtgccgttcg gacgcccccg caccatcgag ctgctgcgct cacccgagtt caacgatctg
    11821 gccttcaagg tgcgcggtct cctcggggag agcatatgaa gacacgcgag gaccccgacg
    11881 tcgccgcggt cgaggctcag cggcttacgg agttggaaac ctcaagcctg gcccggcgcg
    11941 tggggagtgc ctggccggcg ttccttgtct tcgctctctt ggtcgcggcc tgggaggggg
    12001 cggcgcgcgc cgagctcatc agcaggctcg tcctgccggc gcccagtcgg atcttcctcg
    12061 cgctgatcga actattcacg agcgggctca tctgggagca cctgcgggcc acgttcctcg
    12121 agacgatggc gggcttcgcg gtcggggcgg ggtcggcctt cgtcttggcc gtgctctcca
    12181 gcttctgggg atggttccga cagattgttt cgccctacat gatcatcctg caggtcacgc
    12241 cgcgcgtggc cctggccccg atcttcatca catggttcgg gttcgggctc atgcccaaga
    12301 tcgtcatggc cgcgacgatc tgcttcttcc cggtgttcat caacacgctg accggcctgc
    12361 tgaacaccga cgaggaggcg ctggagatgt tccggtcgat gggcgcgagc aagcggcaga
    12421 tcttcacgca gttcacgatg ccgtcggcca tgccggtgac gttcgccggc ctcaagacgg
    12481 gcatcacgct ggcgttgatc ggggcgatcg tcgccgagtt cgtggccacg agccaaggct
    12541 tgggccttct gatcgaccgg ttcaacttcc gcctggcgat ggaggaggcg ttcgccgtgc
    12601 tcctcgtgct ggccctgctc ggcctgctcc tgtacggcgt catggagttc ctcgaccggc
    12661 gcatcgtgtt ctggacgagg gaggagcggc tctccaagag ggagatccgg agtcggaggg
    12721 gaagggagga caccgcatga aacgggcacg cttcatcgcc ctcctcgcgg ccgccgtcct
    12781 ggtggcgtcg gcgtgcggag gggacgatgg cggcggagga gggggcgacg gcgaggtcgt
    12841 cgagatcgat ttcctccagc cgctccccaa gtccatcgcg ttctacccgc tgttcgtcgg
    12901 cgagcggctc ggctacttcg aagaggaggg cgtacgagtc aacctcctgc cgtccggcga
    12961 catcccggcg acggtggccg tgcccgccgg caacgccgac atcggggcaa ccacaccggc
    13021 gaacatcgtg gtcggcgctt cggaagaaga ggacttcgtc ctcgtctacg agtactacca
    13081 gaagaacgtg ttctcgatcg tggtgccgga gaacagcgac gtccaggagg tggccgacct
    13141 cgcgggccgc aacgtcggca tcacctccga gggcgctggg gacgcggcgc tcacccgcgc
    13201 ggcgctcacg caggcggggc tcgaccccga tgcggacgtg acgatcacgg tggtcggcga
    13261 gggggggcct accgtggcca acgcgctcga cgaggggagc atcgatgcgt tcaccggggc
    13321 gatcaacgac atcgttgcgc tgcgcgtggc cggcgtcgag gtccgcaaca tcacgccgga
    13381 gaacctcgac atcctgcccg cctcctcgat gatcgcccag ccttcccaga tccaggagat
    13441 gggtgacgcc atgcagggat tcctcagggc ctgggccaag gccacgtacg cggggatgca
    13501 caaccccgac gcggtgtatg ccatggccca ggaggaagtg cccgaagaga caggggacga
    13561 ggagttcggc aggatcttcc tcgagcaggc gatcgacctg cagacccccg tggagggcac
    13621 ggacaacttc ggggccctcc gccccgacgc ctggtccgcc gtgatggatc agctgatcga
    13681 gggtggggag ctcgaggaaa cgttcgaccc aagctcgttc ctggacgacc gcttcatcga
    13741 gggcgcgaac gacttcgacc gggccgaggt ggaacaggag atcgacgact ggttctcgga
    13801 gaacggttga gcgaggatcg tcgaggggcc gcgggccggc aaggcccgcc gccccaccac
    13861 cccaacaccc aggagcaccc
//
LOCUS       DASUTW010000144         4251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1080832, whole genome shotgun sequence.
ACCESSION   DASUTW010000144 DASUTW010000000
VERSION     DASUTW010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080832"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VF058_04760"
     CDS             <1..187
                     /locus_tag="VF058_04760"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX6207654.1"
                     /translation="AVDELAPHLREAFVLIEVMGLSYRETAVVLGVPPGTLKSRMHAA
                     RKALMESLAFEEDADEV"
     gene            177..2057
                     /locus_tag="VF058_04765"
     CDS             177..2057
                     /locus_tag="VF058_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207655.1"
                     /translation="MRCDAAERELSAALDGEPFDAAARAHVETCTACRRFETEARLLR
                     EAVRLEPAPAVPDLVGPIVERVRAEPPRRGWGRELAAFAAAAVVAALVVGGLPGVRRG
                     AAPVEASEISQRVAEASREITSFRAVFEITERSFADDVPLRRFEARVTFRAPERFLAR
                     VRDRTTYPSEAWPRNDVRLAVDGDRWSLTAPVPCPREALPACASGETRTLAVRGRAPF
                     DGDAPIPTDVILPMKALSSAGRLRVVGEGSISGRSVVRVELDYRDATPLLAFLQAGGS
                     WRPFFPLDRVVVSLDAETWFPLGYRVLATSGAEREAWALRNGLPRERGGATLLEVRAR
                     TFVESAPRGWRPEVEGLGAVDRGFREANRSELPFEPVVPTTLAGLEPYREGLLGSDEI
                     LTSYARGLTWLRIRESRSWDQEALFGDVGPLAAPVRLPGGGFAFHEPPTDDLGHRLAI
                     HAGGTDLYLESNLPFDRLLEVAASAPVRGLPIPDAWRTRRLPGGVQEVGVPLKRAVRV
                     FPGLALPRWLPAEYRLWTLHLFRARSGAAVTAYFRRPGGELDGVGIRLHQAEGTGLPP
                     PTDPGAVGVRIDVEVGRYSPIRGELEWVEDGIYRSLRAEGLDLDHLVAIARSMAPPGG
                     AT"
     gene            2054..3232
                     /locus_tag="VF058_04770"
     CDS             2054..3232
                     /locus_tag="VF058_04770"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX6207656.1"
                     /translation="MRFLRVLVAALVGALVGGAVTLGMPLEDPRPPVRRPPPAELVKQ
                     APIRTLLAWTPGGLPPGFAGAVQRLPSVRVAAEVLSGTAWLTAWRPEGSPRRTPPSGL
                     AYPTEVAAIRPLLYRRFVPPADRPLVESLARGGALLSRGAARLRGIEAGGTMRFGGLV
                     LPVVGVLDDELVGAHEVVVSRETGARLGITRARYLLVAPRAKAERREVEEGIRGALPP
                     GVRVRVRGPGETPVFRHGDAVLPPLRLKELFGEFAAAPAAGGMLRMEAGWVRRNIRTT
                     TLPVIGPATCHRAIVPAMRSALAELERRALDDLIDPGDFGGCYSPRFILRSPGAGISH
                     HGWGIAFDLNVSQNPFGREPRMDPRIVEVFERHGFTWGGRWLVPDGMHFEFLRFAEGG
                     "
     gene            3288..>4251
                     /locus_tag="VF058_04775"
     CDS             3288..>4251
                     /locus_tag="VF058_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207657.1"
                     /translation="MRRLFTSIAVAALVLSTAGPAAAGGWWSFIPLQGAELGVGERFA
                     SGRGETLFLTAEAAARARSGEDPYHAYLIPEIDWRIANESASAREYHSRWARPHAAIR
                     VGSVKVWGGSRNYARFRVVFDVPDATPGPYVLALCSEGCAAPLGDVVPSQITLVADPV
                     AARTAREVGKLDWRVNALGQRANHLERQIRALRGAGTDPEVIEAIDALTVRAEKEARR
                     VQELEARVEELEGAAPAPAWLVVGAAGAALLLLLPRRRRPRGRPPEPPVEMELEALPD
                     SLADDEPIRERGVAPVPPAPAQPAARPLTPVRARAAAPHRAATAR"
BASE COUNT          488 a         1508 c         1671 g          584 t
ORIGIN      
        1 cgccgtggac gagctcgccc cgcacctccg ggaggccttc gtcctgatcg aggtgatggg
       61 gctgagctac cgggagacgg ccgtcgtgct gggcgtgccg cccggcacgc tgaagagccg
      121 gatgcacgcc gcgaggaagg ccctgatgga gagcctcgcc ttcgaggagg acgccgatga
      181 ggtgtgacgc cgccgagcgc gagctctcgg ccgccctgga cggcgagccg ttcgacgcgg
      241 cggcacgcgc gcacgtcgag acgtgcaccg cgtgccgccg cttcgagacc gaggcccgcc
      301 tgctccggga ggcggtccgc ctcgagcccg ccccggcggt gcccgacctc gtgggcccga
      361 tcgtggagcg ggtccgggcg gagccgcctc ggagggggtg ggggagggag ctggccgcgt
      421 tcgccgccgc cgcggtggtg gcggcgctcg tggtgggggg cttgcccggg gtacggcgag
      481 gggccgcccc cgtcgaggcc tctgagatct ctcagcgagt ggccgaggcc tcgcgcgaga
      541 tcacctcctt ccgagcggtg ttcgagatca ccgagcggag cttcgcggac gacgtgccgc
      601 tgcgccgctt cgaggcccgc gtcacgttcc gggctccgga gcgattcctg gcccgggtgc
      661 gggaccgcac gacctatccc agcgaggcct ggccccggaa cgacgtgcgg ctggcggtgg
      721 acggagatcg ttggtcgctg accgcgccgg ttccgtgccc gcgggaggcc ctccccgcct
      781 gcgcctcggg ggagacgagg acgctggccg tccgagggcg ggcgccgttc gacggcgacg
      841 cgccgatccc cacggacgtg atcctcccca tgaaggcgct ctcgagcgcg gggaggcttc
      901 gcgtggtggg cgagggatcg atctcgggcc gctccgtggt ccgggtcgag ctcgactacc
      961 gggatgcgac gcccctgctc gccttcctcc aggcgggcgg atcctggcgc ccgttcttcc
     1021 cgctggaccg cgtggtggtc tcgctcgacg cggagacgtg gttccccctc ggataccggg
     1081 tgctggccac ttccggtgcg gaacgagaag cctgggccct ccgcaacggc ctccctcggg
     1141 agcgcggggg cgcaaccctg ctggaggtgc gggccaggac cttcgtcgaa tcggcacccc
     1201 gcgggtggcg acccgaagtc gagggcttgg gagcggtgga tcggggcttc cgggaggcga
     1261 accggagcga gcttcccttc gagccggtcg tgccgacgac cctcgcgggc ctcgagccgt
     1321 accgcgaagg gctcctggga tcggacgaga tcttgacctc ctacgcccgg ggcctgacct
     1381 ggctccggat ccgtgagagc cggtcgtggg atcaggaggc gctgttcggt gacgtcgggc
     1441 ccctcgccgc cccggtgcgt ctccccggcg gcggcttcgc cttccacgag cccccgacgg
     1501 acgatctcgg ccaccggctg gcgatccacg cggggggcac ggacctctac ctcgagtcca
     1561 acctcccctt cgaccggctg ctggaggtgg ccgcctcggc gccggtgcgc gggctcccca
     1621 tcccggatgc ctggcggacc cgtcgcctcc cggggggcgt gcaggaggtc ggggtcccgc
     1681 tgaagcgggc cgtccgggtc ttcccgggcc tcgccctgcc gcgatggctg cccgccgagt
     1741 accgcctctg gaccctgcac ctgttccggg cgaggagcgg ggcggccgtg accgcctact
     1801 tccgccggcc ggggggcgag ctcgacggcg tggggatccg cctccaccaa gccgagggga
     1861 cgggcctccc tcctcccacg gacccgggag cggtcggggt ccggatcgac gtcgaggtcg
     1921 ggaggtactc accgatccga ggcgagctgg aatgggtcga ggacgggatc taccgctcgc
     1981 tgcgcgcgga gggcctcgac ctggatcatc tcgtggcgat cgcgcggtcg atggcgcccc
     2041 ccggaggcgc gacgtgaggt tcctccgcgt cctcgtggcc gccctcgtcg gtgcgctggt
     2101 cggtggcgcc gtgacgctgg ggatgccgct ggaggaccct cgcccgccgg tccgccggcc
     2161 cccaccggcg gagctcgtca agcaggcgcc gatccgcacg ctcctggcct ggacgcccgg
     2221 gggcctcccc cccggcttcg cgggcgccgt gcagcgcctt ccgtcggttc gggtggcggc
     2281 cgaggtcctg agcggcacgg cgtggctcac cgcctggagg ccggaggggt cgccgcgcag
     2341 gacgcccccg tcgggcctgg cctacccgac cgaggtggcg gcgatccggc cgctcttgta
     2401 ccggcgcttc gtcccccccg cggacaggcc cctcgtcgag tcgctggccc gaggaggggc
     2461 gctcctgagc cgcggggcgg cccggctgcg aggcatcgag gcggggggca cgatgcgctt
     2521 cggagggctg gtcctcccgg tggtgggggt gctggacgac gagctcgtgg gggcgcacga
     2581 ggtcgtggtc tcccgggaga ccggagcgcg gctggggatc acgcgggccc gctacctgtt
     2641 ggtggcgccg cgagcgaagg cggagcggcg agaggtcgag gaggggatcc gcggggcgct
     2701 cccgcccggg gtccgggtcc gggtccgagg tcccggcgag actccggtct tccgccacgg
     2761 cgacgcggtg ctgccgcccc tgcgcctgaa ggagctgttc ggcgagttcg cggcagcccc
     2821 ggcggcgggg gggatgctga ggatggaggc cggctgggtg cggaggaaca tccggacgac
     2881 gacactccct gtcatcgggc ccgcgacctg ccaccgagcg atcgtgcctg cgatgcgctc
     2941 ggccctcgcc gagctggagc gacgggccct cgacgatctc attgatccgg gcgacttcgg
     3001 gggctgctac tcgccgaggt tcatccttcg gagcccgggt gccggcatct cgcatcacgg
     3061 ctgggggatc gccttcgacc tcaacgtctc gcagaacccg ttcgggcggg agcctcggat
     3121 ggatccgcgc atcgtcgagg tcttcgagcg ccacggcttc acctggggag ggcggtggct
     3181 ggtgccggac gggatgcact tcgagttcct gaggttcgcc gagggcggct gaaccttcgg
     3241 cggcgggcgc gcgtcggtac gagcagacca tgaggaagga ggcgcccatg cgacgtctgt
     3301 tcacgtccat cgcggtcgcc gccctggtgc tgtcgacggc cgggcccgcc gccgccggcg
     3361 gatggtggtc cttcatcccc ctccagggag ccgagctggg ggtgggcgag cgcttcgcgt
     3421 cgggccgagg agagacgctc ttcctgacgg cggaggcggc ggcccgcgcc cggagcgggg
     3481 aggatccgta ccacgcctac ctgatccccg agatcgactg gaggatcgcg aacgagtccg
     3541 cgagcgctcg cgagtaccac agccgatggg cgaggcctca tgcggcgatc cgcgtcgggt
     3601 cggtgaaggt ctggggcggg tcgaggaact acgcgaggtt cagggtcgtc ttcgacgtgc
     3661 ccgacgcgac ccccgggccc tacgtcctgg cgctctgcag cgaaggctgc gcggcgccgc
     3721 tcggcgacgt cgtccccagc cagatcaccc tggtggcgga cccggtcgcg gcacgcacgg
     3781 cgcgagaggt ggggaagctc gactggcggg tgaacgccct ggggcaacgg gccaaccacc
     3841 tggagcgcca gatccgtgcg ctccgcggag cggggaccga tcccgaggtg atcgaggcga
     3901 tcgacgccct gacggttcga gcggagaagg aggcccggcg ggtccaggag ctggaggccc
     3961 gggtggagga gctggagggg gcggcgccgg cgccggcctg gctcgtcgtg ggcgccgcgg
     4021 gcgccgcgct gctgctgctc ctcccgcgcc ggcgccgccc ccgcgggcgc ccgcccgagc
     4081 ccccggtcga gatggagctc gaggccttgc ccgactcgct cgcagacgac gagcccatcc
     4141 gggagcgagg ggtcgccccc gtccccccgg cccccgctca gccggcggcg cggccgctca
     4201 cgcccgtccg ggcgcgagcg gccgcgccgc atcgtgcggc caccgccagg g
//
LOCUS       DASUTW010000145        11335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_693804, whole genome shotgun sequence.
ACCESSION   DASUTW010000145 DASUTW010000000
VERSION     DASUTW010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11335
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693804"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..565)
                     /locus_tag="VF058_04780"
     CDS             complement(26..565)
                     /locus_tag="VF058_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207658.1"
                     /translation="MWIVIVVAAVLVIAAIAWAVWERRRREGLRDRYGPEYERLEEDE
                     GARHAAAELRERERRRETFDIRPLSSEQRSRFAGAWEDLQARFVDAPNVAVLEADRLV
                     IEVMRAKGYPMDDFDQRAADLSVDHPRVVENYRTAHDVAMRSERGEADTEELRRATLA
                     YRELFEELLEEEPGYERAG"
     gene            complement(679..3387)
                     /locus_tag="VF058_04785"
     CDS             complement(679..3387)
                     /locus_tag="VF058_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013733552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEX6207659.1"
                     /translation="MIVAATVAMIALLFVPVPGTSVAQSEVSVNEAIDIAFDHVQGSP
                     GDLGVTPEDVAELEVASAYRSQHNRVVHVNLRQFHEGDPVGNSHVTVNVAGDGTVLFV
                     GHNLESVSEDEGDVALDALAAVRAAADALGLEPDGLRIVSRRGGVFGDAVVSRGGISD
                     APIPAALVWEPTEDGLRMAWDLVIDDATTASLWNVAVDAVTGELLRQDDWTDHDTLAG
                     LTSAVARTQVSSGDPTFLEPNNPVNEGSSYRVFQAPTESPNDYDRRLVENPAEETASP
                     FGWHDTDGAMGPEFTITRGNNVHAYTDHDNSNDPDESKTVVTVDPPSSAAGEYAAVQA
                     NFGPDIPMGGISGDIELGDDGTGPNVNDGCEPYTVTAGAIALVDRGQCNFTVKVLNGQ
                     NGGAAAVIVANNLPTPPFGMGGNDPAITIPSVMISMDDGTTIKGGLPATGSMEVIPLP
                     NPSAPDGGPGLDFDYPIDLTTEHPHDYWDAAVTNLYYMNNVIHDVMFGYGFDEVAGNF
                     QVTNYTGEGNGGDDVRAEAQDGGGQNNANFATPAMDGGRPRMQMYLWTLGTPVRDGDF
                     ENGIIIHEYGHGISNRLVGGPTVNCITGQERMGEGWSDYFAISMLIDPTLDDPDGPRG
                     MGPYALFQNSRQDAGIRPRPYSRDMTIQPFTYDRVKTNGWITGGSLAMPHGVGHAWAA
                     TLWDLTWNLIDRHGFNPNIYDDWSTGGNNLAIQLVTDGLKFQGCGPGFVVGRDAIIAA
                     DQALTGGENECIIWASFARRGLGFSAVQGTTDRNDNTEAFDTHPACAADGTGFSPPVS
                     NTALNTFDAGETVPLMFNIGGDRGPNPLASNSPASQEIDCTTRTALQYAITTNTDSTS
                     GKLTYNKNLQRYHYNWATSADWAGTCRQVIITLDDGTQLRADFRFE"
     gene            complement(3763..5436)
                     /gene="arc"
                     /locus_tag="VF058_04790"
                     /pseudo
     CDS             complement(3763..5436)
                     /gene="arc"
                     /locus_tag="VF058_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016188838.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
     gene            complement(5504..6289)
                     /locus_tag="VF058_04795"
     CDS             complement(5504..6289)
                     /locus_tag="VF058_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEX6207660.1"
                     /translation="MSDPLAAGERALLVDARGRRFLITLETGGEFHFHGGVVAHDDVI
                     GRPEGSRIESRSGSALTAFRPRLADFVLKMSRGAQVVYPKDLGAILIQADVYPGARVL
                     EAGTGSAALTIALCRATGPEGRVVSYELREDHREVAERNVASFFGRAPAWLELRAGDV
                     RDVAGTGERFDRAVLDLPEPWEAVPEVTRALDPGAVLCGYLPTTPQVQSLVVAMVDRG
                     YREVEAFEVLQRGWHVTARSVRPHHRMVAHTGFLVVGRRSADA"
     gene            complement(6286..7362)
                     /locus_tag="VF058_04800"
     CDS             complement(6286..7362)
                     /locus_tag="VF058_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX6207661.1"
                     /translation="MSTRARTAALLLAGSLLITACAGGPGITIEAPEQAAETAATPSP
                     IPPLDPDEPIPAVVERVSPAVVNVRVNQAGGASGTGTGFVVRPNGIIVTNFHVVDGAF
                     SIQVTLADGREFEARAIGGDPNADLAILQIEAQELPTVELGDSDALRLGETVIALGFA
                     LDIPGGPSVTSGIVSAKGRTIEAAGPQGPNTLEDLIQTDAPINPGNSGGPLVDLAGRV
                     VGINTAGVSAAEAENIGFAIAINRARPIIEEAIDAPEAEAAYLGVSTQDVTPVVAAQL
                     DLPVDEGALVRGVAPGTGAEEAGIVAGDVIVEIEGQAIASSADLGDAIRDHAPGDEIS
                     ITVIRDGEPRTVTAELGVRPLPTG"
     gene            complement(7402..8184)
                     /gene="fabI"
                     /locus_tag="VF058_04805"
     CDS             complement(7402..8184)
                     /gene="fabI"
                     /locus_tag="VF058_04805"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX6207662.1"
                     /translation="MRTMLEGKRLLVTGVLTPQSIAFHCAKQVQELGAEVLLTGFGRG
                     MSITEKSAKRLPEPADVLEMDVNDPEHVTGVAAAVGDRWERLDGLLHAIAFAPQDALG
                     GRFLETEWPSVATALQTSAYSYKALVTAFLPLLKGGGSAVTLDFDASVAWPGYDWMGV
                     AKAALEAVTRYLARDVGREGVRVNAVSAGPLKTVAAKGIPGFDVFTDLWYKRAPLGWD
                     VKDPSPVAGAVAFLLSDHSRGITGEILHVDGGYHAMGADLRE"
     gene            complement(8186..9418)
                     /locus_tag="VF058_04810"
     CDS             complement(8186..9418)
                     /locus_tag="VF058_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015144649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEX6207663.1"
                     /translation="MGLNAEAARKRFPALERTIEGRPCVFADAPAGTQVPDVVIDAMA
                     DYLRRSNANVGGAFATSLETDEAIAAARRSAADLLGCDPGEVVFGPNMTTLSLALSRA
                     VARELGPGDELVVTVLDHDANVAPWVLAAEDAGATVRWADLDEEDCTLDLASLERALG
                     ERTRVVALTLASNAVGSVTPVDEVVTIVRERAPEAILVGDAVHLAPHRSIDAGPFDAV
                     LCSPYKFFGPHLGIMAARRELLERLRPYKVRPAPDEPPDRWETGTQSHEALAGLTAAI
                     GYLRDLVEGEGRPGVLAAMEEIRRHEAVLSGRYLEGIARIPAVRLFGIADPERVDERT
                     PTFAIRVGGQHPRSTAEELARRGIFVWDGNYYALAIMERLGLEDEGGAVRIGFCHYHT
                     EDEVDRVVEEVAALAAAP"
     gene            9495..11282
                     /locus_tag="VF058_04815"
     CDS             9495..11282
                     /locus_tag="VF058_04815"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEX6207664.1"
                     /translation="MRARLTRGRVAAVAIAVLMVPLVGASASAEPGTATLDASSHRVT
                     YGEPVSVQGVLSTADVCLVGRSVALQRRPPGMVSWEEVATASTGAGGAFSFQTFPDEI
                     ADYRALIAPETRETVSCDEVLSQVVGIDVRAAVTLALSGNPVPASGCARLRIDVLPTK
                     PGQEVLLQRRRGGSWTTEETLILGPDSEASRRTCMTWSEIGRHRFRARWPRQDESNLA
                     ATSSPVPMRVVKAAWMKRIDAIAAPLRMGVSVRERGAFLFRRGDTGLRTPASNQKLLL
                     SMALLDTLGPDRRLATRALSRRPRNGVVRGHLWIVGSGDPEVGPSKLRRLAARIEEAG
                     VRRITGGVRGSTALFARDWWAPGWRGYFPRTVIALPTALTFRGNVAGGRHVRDPERRA
                     ALALTKQLRRRGVRVAKPPGMGRHPSGRPELTRVESRPLSGILTITNHASSNFWAEVL
                     TKGLAVARREPPGTIAKGAGAVAAWVRANGGRSRSHDGSGLSYANRTTPESIARLLGR
                     AEGETWGARLRSTLPTGGVGTLSGRLHRVPVRAKTGTLSGISALSGWVESERTATWVE
                     FSLLSQGVSTTRAKAAEDRIVRILWRNAR"
BASE COUNT         1496 a         4093 c         3936 g         1810 t
ORIGIN      
        1 tcccggcgcc cgttggggct cgacgttagc ccgcccgctc gtaccccggc tcctcctcca
       61 gcagctcctc gaagagctcg cggtacgcca gcgtggccct ccggagctcc tcggtgtccg
      121 cctcgcccct ctcggagcgc atggcgacgt cgtgggcggt gcggtagttc tccaccactc
      181 tcgggtggtc gacggagaga tcggccgccc gctggtcgaa gtcgtccatc gggtagccct
      241 tcgcccgcat gacctcgatc accagacggt ccgcctccag caccgcgacg ttcggagcgt
      301 ccacgaaccg tgcctggaga tcctcccacg cgccggcgaa gcgcgaccgc tgctcggagc
      361 tgagggggcg gatgtcgaag gtctcccgtc gacgctcccg ctcgcgcagc tcggccgccg
      421 cgtgccgcgc tccctcatct tcctcgagcc gctcgtactc cggcccgtat cgatcgcgga
      481 gcccctcgcg gcggcgacgc tcccagacgg cccacgcgat cgcggcgatc accagcaccg
      541 ccgcgaccac gatcacgatc cacacccatg catccatgtc tcttcacccc cgggcggctc
      601 gtacccgagg gctgcacggg tgaaaccgga gcagaggagg gagggggccg ggcggccccc
      661 tccctcatgc cgcgactcct actcgaaccg gaagtccgct cggagctggg tcccgtcgtc
      721 cagggtgatg atcacctggc ggcaggtccc ggcccagtcc gcggacgttg cccagttgta
      781 gtggtaccgc tggaggttct tgttgtacgt cagcttccca ctcgtggagt cggtgttcgt
      841 ggtgatcgcg tactgcagcg cggtccgcgt cgtgcagtcg atctcctgcg acgccggcga
      901 gttgctggcg agcgggttcg gcccgcggtc gccgccgata ttgaacatca gcgggaccgt
      961 ctcccccgca tcgaacgtgt tcaacgccgt gttcgacacg ggcggtgaga acccggttcc
     1021 gtccgctgca cacgctgggt gcgtgtcgaa cgcctcggtg ttgtcgttac ggtccgtcgt
     1081 gccctggacc gcgctgaagc caaggccgcg gcgggcgaac gacgcccaga tgatgcactc
     1141 gttctcgccg ccggtcagag cctggtcggc cgcgatgatc gcgtcacggc ccaccacgaa
     1201 gccgggaccg cagccctgga acttcagccc gtcggtcacg agctggatcg ccaggttgtt
     1261 ccccccggtg ctccagtcgt cgtagatgtt cgggttgaag ccgtggcggt cgatcaggtt
     1321 ccaggtcagg tcccagaggg tcgccgccca cgcgtggccg acgccgtgcg gcatcgccag
     1381 tgaccctccg gtgatccacc cgttcgtctt gacgcgatcg tacgtgaacg gctggatcgt
     1441 catatccctg gagtacgggc gcggtcggat cccggcgtcc tgcctgctgt tctggaacag
     1501 ggcgtacggc cccatgcccc gcggaccgtc cgggtcgtcg agcgtggggt cgatgagcat
     1561 cgagatggcg aagtagtcgc tccagccctc gcccatccgc tcctgcccgg tgatgcagtt
     1621 gacggtcggg cctccgacga ggcggttgga gatgccgtga ccgtactcgt ggatgatgat
     1681 gccgttctcg aagtcgccgt cacgcacagg cgtcccgagc gtccacaggt acatctgcat
     1741 cctcggacgg ccgccgtcca tggccggcgt ggcgaagttg gcgttgttct ggccgccgcc
     1801 gtcctgggcc tcggcccgga cgtcgtcgcc gccgttcccc tcgcccgtgt agttggtgac
     1861 ctggaagttg ccggcgacct cgtcgaaccc gtatccgaac atgacgtcgt ggatgacgtt
     1921 gttcatgtag tagaggttcg tgaccgcggc atcccagtag tcgtgcgggt gctcggtcgt
     1981 gaggtcgatc gggtagtcga agtccaggcc cggtccgccg tccggcgcgg atgggttcgg
     2041 cagcgggatg acctccatgc tgcccgtcgc cgggagaccg cccttgatcg tcgtcccgtc
     2101 gtccatcgag atcatcaccg acgggatcgt gatcgcgggg tcgttcccgc ccatcccgaa
     2161 cgggggcgtg ggcaggttgt tggccacgat gaccgcggcc gccccgccgt tctgcccgtt
     2221 cagcaccttc acggtgaagt tgcactgccc gcggtcgacg agcgcgatcg cgccggcggt
     2281 aaccgtgtag ggctcacacc cgtcgttcac gttcgggccg gtgccgtcgt cgcccagctc
     2341 gatgtctccg gagatcccgc ccatggggat gtccggcccg aagttggcct ggaccgcggc
     2401 gtactccccg gccgccgatg acggcgggtc gacggtcacc accgtcttgc tctcgtccgg
     2461 gtcgttgctg ttgtcgtggt ccgtgtacgc gtggacgttg ttgccgcgcg tgatcgtgaa
     2521 ctccggaccc atcgcgccgt ccgtgtcgtg ccagccgaac ggcgacgccg tctcctcggc
     2581 ggggttctcg accaggcgtc ggtcgtaatc gttcgggctc tcggtcggcg cctggaacac
     2641 gcggtagctg gagccctcgt tcaccgggtt gttcggctcg aggaacgtcg ggtcgccgga
     2701 cgagacctgc gtgcgggcga ctgcgctcgt gaggcccgcc agcgtgtcgt gatccgtcca
     2761 gtcatcctgg cgcagcagct ctccggtcac cgcgtcgacc gccacgttcc agaggctcgc
     2821 ggtcgtcgcg tcgtcgatga cgaggtccca ggccatccgc agcccgtcct cggtcggctc
     2881 ccagaccagg gccgccggga tcggcgcgtc cgagatcccg cccctggaca cgacggcgtc
     2941 cccgaagaca ccgccccgcc ggctcacgat ccggagcccg tcgggctcga ggccgagcgc
     3001 gtcggcggcg gctcgcacgg ccgccagggc gtcgagggcg acgtcgccct cgtcttcgga
     3061 caccgattcg aggttgtggc cgacgaacag cacggtgccg tctcccgcga cgttcacggt
     3121 gacgtgcgag ttccccaccg ggtcgccctc gtggaactgg cgcaagttga catggaccac
     3181 ccggttgtgc tggctccggt acgcgctcgc gacctcgagc tccgcgacgt cctcaggcgt
     3241 cacgccgaga tccccggggc ttccctggac gtggtcgaag gcgatgtcga tcgcctcgtt
     3301 cacggagacc tcgctctgcg cgacggacgt gccgggtacc ggtacgaaga ggagcgcgat
     3361 catggcgacc gtcgccgcta cgatcacgct tcgctttctt ggcgacatcc cttacctccc
     3421 tccgccccga ggggcgaccg ggcggccgct gtgggcttgc tgcgcggccg caacggaaca
     3481 cgcgacgttg gaaggtcccg tcggggaggg aggtccgcgg atcgtgggca cgcagagcga
     3541 acgcagaggc cgcacgcagc acccgttgcg tcgcatccgt cacgaacccc cggtgatcgg
     3601 agcactgcgg gcagcgatgg cccggggcca tcgccgacca aacctacgga atgggatgta
     3661 tcggagtcaa atcgagcgcc gagtgcgctc cgcgttcgcc tacaggtact ggccgggctg
     3721 gatgcggtcg acctggcggc tgcccgagcc gtcggcctcg cccagcaggg tgcggacgta
     3781 gacgatccgc tcgcccttct tccctgagat ccgggcccag tcgtcggggt tcgtcgtgtt
     3841 gggcaggtcc tcgtgctcct tgaactccgc ccggatggcg tcgaggaggt gctgcagctt
     3901 gatccccttc tcgccgatct ggaggaactg cttgatcgcc gacttcttgg cccgggccac
     3961 gatgttctcg atcatcgctc cggagttgaa gtccttgaag tagaggatct ccttgtcgcc
     4021 gttctggtag gtgacctcca ggaagcggtt ctcccctgtc tcggcgtaca tcgcatcgat
     4081 cgtgcggtcg atcatcccgg gcagggcctg cgtcttgtcg ccgccgtagg ccttcagctc
     4141 gtcggggtgg atcgggacgt cgggcgtgag gtacttcgac atgatgttgc gcgcggcctc
     4201 gatgtcggga cgctcgatct tgatcttcac gtcgagtcgc cccgggcgga ggatcgccgg
     4261 gtcgatcatg tcctcgcggt tcgaggctcc gatcacgatc acgttcttca gggtctcgac
     4321 gccgtcgatc tcggccagca gctgggggac gatcgtgttc tccacgtccg aggagatccc
     4381 cgaccctcgc gtgcggaaga tcgagtccat ctcgtcgaag aacacgatca cggggaagcc
     4441 ctcctgggcc ttctccttcg cccgctggaa gatcacgcgg atctgccgct cggtctcgcc
     4501 cacgtacttg ttgagcagct cgggtccctt gacgttgagg aagtacgagt gagcctcggg
     4561 gcggccggtc ttctgggcca cctgcttggc cagcgagttg gccacggcct tcgcgatcag
     4621 ggtcttcccg cagcccggcg ggccgtagag caggacgccc ttcggggggc gcagccgatg
     4681 ctcccggaac agctccgcgt acaggaacgg catctccacg gcgtcgcgga tgtcgtcgat
     4741 ctgcgacgcg agcccgccga tgtcgtcgta ggtgacgtcg gggacttcct cgaggacgag
     4801 ctcctcgatc tcctccttgg ggaggcgctc cagcacgtgg ccggagcgag gatcgtagag
     4861 gagggcgtcg ccggctcgga agtgctcgcc ctggaggcgc ttggagacga tcgccaccat
     4921 ctcctcgtcc ccccggccca gcacgatcac gcggccgtcg cccaggatct ccttcacgct
     4981 gacgacctcg ccctggcggt cctgctccag cacctccacc acgttgaggg cctcgttgag
     5041 gatgacctcg gcgccgcggt gcaggaggga gcgatccacc tcagggctga cgttgacccg
     5101 catctttcgg ccggacgtga agacgtcggc ggaaccgtcc tcgttctcgc ggacgaacac
     5161 gccgaaggag gcggggggcc gggtgagctt gtcgacctcc tccttgaggg cctcgatgcg
     5221 ctcccgctcg gtcttgaggg cgcccacgag cttctcgttc tgggccagcg ccttggcgac
     5281 ctgggacttc gactccagga ggcgctcctc gagcaccttc acctggcgcg gtgtggagtg
     5341 ctggcgctgc cgcagcacgg ccagctcgtc ctccaggaat cgcacctggg tctggagctc
     5401 gtggatctct ttctcgacgt cgcggtccgg ctgcatcggg tgcgggtcct cccctcgagg
     5461 cccaagtata gcggcggcct cccgatgggt gatcgaaggg cgctcaggcg tcggcgctgc
     5521 gacggcccac gacaaggaag ccggtgtggg ccaccatccg gtggtgtggg cggacgcttc
     5581 gggccgtcac gtgccagccc cgctggagca cctcgaaggc ctcgacctcg cggtagccgc
     5641 ggtcgaccat ggccacgacg agtgactgca cctggggcgt ggtcggcagg tagccgcaca
     5701 ggaccgcacc ggggtccagt gccctcgtga cctcggggac ggcctcccac ggctcgggga
     5761 ggtcgaggac cgcgcggtcg aaacgctccc ccgtccccgc cacgtctcgg acgtcgccgg
     5821 cccggagctc gagccaggcg ggggcccggc cgaagaacga cgcgacgttc cgctcggcga
     5881 cctctcggtg gtcctcccgg agctcgtagg agacgacccg gccctccggg ccggttgccc
     5941 ggcagagcgc gatcgtgagg gcggccgacc ccgtgccggc ctccaggacc cgcgcgccgg
     6001 ggtagacgtc cgcctggatc aggatcgcgc ccaggtcctt cgggtacacg acctgcgccc
     6061 ctcgcgacat cttcaggacg aagtccgcga gccgggggcg gaacgcggtg agggcgctcc
     6121 cgctccgcga ctcgatccgg gagccctcgg gcctcccgat cacgtcgtcg tgcgccacca
     6181 cccccccgtg gaagtggaac tcgcctccgg tctccagggt gatgaggaac ctgcgcccgc
     6241 gggcgtccac gaggagcgct cgctcccccg ccgccagcgg gtcgctcatc cggtcgggag
     6301 gggcctgacc cccagctcgg ccgtgacggt gcggggctct ccgtcccgga tcaccgtgat
     6361 cgagatctcg tcgcccgggg cgtggtcccg gatcgcgtcg ccgagatccg cgctgctcgc
     6421 gatcgcctgg ccctcgatct cgacgatcac gtctcccgcc acgatgcccg cctcctcggc
     6481 accggtcccg ggcgcaaccc cccgcacgag ggcgccctcg tcgacgggga ggtcgagctg
     6541 cgccgcgacg acgggggtga cgtcctgggt cgagacgccc aggtacgccg cctcggcctc
     6601 gggcgcgtcg atcgcctcct cgatgatggg cctggcccgg ttgatggcga tggcgaagcc
     6661 gatgttctcg gcctcggcgg cgctgacccc cgccgtgttg atcccgacca cgcggccggc
     6721 caggtcaacg agcgggcccc cggagttccc ggggttgatc ggggcgtcgg tctggatcag
     6781 gtcctcgagc gtgttggggc cctggggccc cgcggcctcg atcgtccggc ccttggcaga
     6841 gacgatgccc gacgtcaccg aggggccgcc cgggatgtcc agggcgaacc ccagcgcgat
     6901 gaccgtctcc cccaggcgca gggcgtcgga gtccccgagc tcgacggtgg gcagctcctg
     6961 agcctcgatc tggaggatcg cgaggtcggc gttggggtcg cccccgatcg cccgcgcctc
     7021 gaactccctc ccgtcggcca gggtcacctg gatgctgaag gcgccgtcca cgacgtggaa
     7081 gttcgtgacg atgatcccgt tcggacggac gacgaagccc gtcccggtcc ccgaggcacc
     7141 gcccgcctgg ttcacccgga cgttcaccac ggccgggctg acccgctcca cgacggccgg
     7201 gatcggctcg tcggggtcga gcggagggat cggcgacggg gtcgccgcgg tctcggccgc
     7261 ctgctccggg gcctcgatcg tgatcccggg accgcccgcg cacgccgtga tcagcaggga
     7321 gccggcgagc agcagggcgg ccgtcctcgc cctcgtgctc atcccgccga ggatagcatc
     7381 gcgcccggcg gaggcccggc gctattctcg caggtcggcc cccatcgcgt ggtagccgcc
     7441 gtccacgtgg aggatctcgc cggtgatccc ccgcgagtgg tcgctcaaca agaaagccac
     7501 ggccccggcc acgggcgacg ggtccttcac gtcccacccg aggggcgcgc gcttgtacca
     7561 gaggtcggtg aagacgtcga agccggggat ccccttcgcc gcgacggtct tgagcggccc
     7621 cgccgacacc gcgttgacgc ggacgccctc gcgacccacg tcgcgggcga ggtaccgggt
     7681 cacggcctcg agcgcggctt tcgccacccc catccagtcg tagccgggcc aggccaccga
     7741 ggcgtcgaag tcgagggtca cggcgctgcc accccccttg agcaggggca ggaacgccgt
     7801 gaccagcgcc ttgtacgagt aggcgctcgt ctggagggcg gtcgccacgc tgggccactc
     7861 cgtctccagg aaccttccgc cgagggcgtc ctggggcgcg aaggcgatcg cgtggaggag
     7921 gccgtcgagg cgctcccagc ggtcccccac cgccgccgcc accccggtca cgtgctctgg
     7981 atcgttcacg tccatctcca ggacgtcggc gggctcgggc aggcgcttcg cgctcttctc
     8041 ggtgatcgac atcccgcgac cgaagccggt gagcagcacc tcggcgccca gctcctggac
     8101 ctgcttggcg cagtggaagg cgatgctctg gggcgtcagc accccggtga ccagcagccg
     8161 cttgccctcg agcatcgtgc gcatcctacg gagccgcggc cagcgccgcc acctcttcca
     8221 ccacgcgatc aacctcatcc tcggtgtggt agtggcagaa gccgatccgg accgccccgc
     8281 cctcgtcctc cagcccgagc cgctccatga tcgccagtgc gtagtagttg ccgtcccaga
     8341 cgaagatccc ccggcgggcc agctcctcgg cggtggaccg ggggtgctgg ccgcccaccc
     8401 ggatcgcgaa cgtcggggtg cgctcgtcga cgcgctcggg atcggcgatg ccgaagagcc
     8461 gcacggcggg gatgcgggcg atcccctcga ggtagcgtcc ggagaggacg gcctcgtgcc
     8521 ggcggatctc ctccatggca gccaggaccc ccggacggcc ctctccctcc acgagatcgc
     8581 ggaggtagcc gatcgcggcc gtcaggccgg ccagggcctc gtggctctgg gtgccggtct
     8641 cccaacggtc gggcggctcg tcgggagccg gccgaacctt gtacgggcgg agtcgctcca
     8701 gcagctccct cctcgccgcc atgatcccca ggtgcggacc gaagaacttg tacggggagc
     8761 agagcaccgc gtcgaacggt ccggcgtcga tcgagcggtg cggcgccagg tggaccgcgt
     8821 cccccacgag gatcgcctcc ggcgcccgct cccgcacgat cgtgaccact tcgtccacgg
     8881 gggtgacgct tcccacggcg ttcgaggcga gcgtcagcgc gacgacgcgg gtgcgctcgc
     8941 ccagggctcg ctccaggctc gccaggtcga gcgtgcagtc ctcctcgtcg aggtcggccc
     9001 atcggacggt cgctccggcg tcctcggcgg ccaggaccca cggggccacg ttcgcgtcgt
     9061 gatcgaggac ggtcaccacg agctcgtcgc ccggtccgag ctctcgggcc accgcccgcg
     9121 acagggccag gctcagggtc gtcatgttcg ggccgaacac cacctccccc ggatcgcagc
     9181 ccagcagatc ggcggcggat cgcctggccg cggcgatcgc ctcgtcggtc tcgaggctcg
     9241 tggcgaaggc cccgccgacg ttggcgttgc tccggcgcag gtagtcggcc atcgcgtcga
     9301 tcaccacgtc gggcacctgg gtcccggcag gcgcgtccgc gaagacgcac gggcgaccct
     9361 cgatcgtccg ctcgagggcg gggaaccgct tccgcgcggc ctccgcgttc agccccatgg
     9421 ccgacagcgt cgcgcatccc cccggccgcc gccacccgtc ttgacgaccc ggccctgacc
     9481 gccacaatgg gccggtgcgc gctcgcctca cccgcggtcg ggtcgcggcg gtggccatcg
     9541 ccgtgctgat ggtgcccctc gtcggcgcgt ccgcgtcggc cgagccgggc acggcgacgc
     9601 tggacgcttc ctcccaccgc gtgacctacg gcgagcccgt gtcagtgcag ggcgtcctct
     9661 ccaccgccga cgtctgcctc gtgggccgct ccgtggccct ccagcgacgc ccgccgggca
     9721 tggtgagctg ggaggaggtc gcgaccgcct cgacgggagc aggaggggcc ttctccttcc
     9781 agactttccc cgacgagatc gccgactacc gggcgctgat cgcgcccgag acgcgcgaga
     9841 cggtctcctg cgacgaggtt ctctcccagg tggtggggat cgacgtgcgg gccgccgtca
     9901 ccctggctct ctccgggaac ccggtccccg cctccggctg cgcccggctc cggatcgacg
     9961 tcctcccgac caagccgggg caggaggtgc tgctccagcg ccgtcgcggg ggctcctgga
    10021 ccaccgagga gacactgatc ctcgggccgg actccgaggc atcgcgccgg acctgcatga
    10081 cgtggagcga gatcggacgt caccggttcc gagcgcgctg gccccggcag gatgagtcga
    10141 atctcgcggc gacctcctcg cccgttccca tgcgggtcgt gaaggcggcg tggatgaaga
    10201 ggatcgatgc gatcgccgcc cccctccgga tgggcgtgtc ggtgcgcgag cggggcgcct
    10261 tcctcttccg gcgtggggac accggcctcc gcacgcccgc gtcgaaccag aagctgctgc
    10321 tctcgatggc gctcctcgac acgctgggcc cggatcggag gctcgccacc agggcgctct
    10381 cccgccggcc ccggaacggg gtggtccggg ggcacctctg gatcgtgggg tccggcgatc
    10441 cggaggtcgg cccctcgaag ctgcggaggc tcgctgcgcg catcgaggag gccggcgttc
    10501 ggaggatcac cgggggcgtg cggggcagca cggccctctt cgcccgcgac tggtgggcgc
    10561 cgggctggcg cggctacttc ccgcgcacgg tgatcgcgct tccgacggcg ctcacgttcc
    10621 gcgggaacgt ggccggcgga aggcacgtcc gcgaccccga gcgcagagcg gctctggccc
    10681 ttaccaagca gctcaggaga cgaggggtgc gcgtggcgaa gccgcccggg atggggcggc
    10741 acccctccgg ccgccccgag ctgacacggg tcgagtcacg tcctctctcg ggcatcctga
    10801 cgatcacgaa ccacgcgtcc tcgaacttct gggccgaggt cctgacgaag ggcctggccg
    10861 tggcccggcg cgagccgccc ggcacgatcg ccaagggggc cggcgcggtc gcggcgtggg
    10921 tgagggcgaa cggagggcgg tcccgcagcc acgacggctc ggggctctcc tacgcgaacc
    10981 gcacgacccc cgaatcgatc gccaggctgc tcggtcgggc cgagggcgag acctggggtg
    11041 cccgcctccg ctcgaccctc cccaccggtg gggtcggcac cctctcgggc cggctccacc
    11101 gcgtcccggt ccgcgccaag acgggcacgc tgagcggcat cagcgctctc tcaggatggg
    11161 tggagtcgga gcggacggcc acgtgggtcg agttctccct gctgtcgcag ggcgtgtcca
    11221 ccacacgggc caaggccgcc gaggaccgca tcgtgcgcat cctctggcgc aacgcccgct
    11281 aggcgcgcac gcagtcgtcg agcaggtccg ggtcgatgtt ggagccggtg ccgat
//
LOCUS       DASUTW010000146         4953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1081765, whole genome shotgun sequence.
ACCESSION   DASUTW010000146 DASUTW010000000
VERSION     DASUTW010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081765"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /gene="nadA"
                     /locus_tag="VF058_04820"
     CDS             complement(<1..327)
                     /gene="nadA"
                     /locus_tag="VF058_04820"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX6207665.1"
                     /translation="MSIVQPDLRDPALESFEAFQGAVRSLAEERDAVILAHNYELPWI
                     QDVADVVGDSLELSRKAAETGASTIVFCGVHFMAETAKILSPDRTVLIPDLRAGCSLA
                     ESITADQ"
     gene            complement(350..1564)
                     /locus_tag="VF058_04825"
     CDS             complement(350..1564)
                     /locus_tag="VF058_04825"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6207666.1"
                     /translation="MSDPVVIVGGGLAAGAAVQTLREEGEDGPIVLIGAESHLPYERP
                     PLSKEYLRGEADAAALPLHQDASYEEWGIDARIGVRATGLDVADRTVELEDGSTVGYR
                     VLLLATGGRARILGAEPSDRVCYLRTVEDADRLRRHLGGGRHLLVVGAGFIGSEVAAS
                     ARALGTDVTMIERAASPLNRVLGEGMGRVLAEIHRERGVDLRLETGLTEVRETGESVI
                     VETDEGDALEGDAVVIGAGIIPNTELAEAAGLEIDNGIAVDARCAASAERVFAAGDVA
                     AHDHPVFGRIRVEHFDNALKMGAHAAKAMLGSPEPFDDAHWFWSDQYDVNLQMGGFAA
                     TWDEIVVRGSVEDRDFTAFYMKDGMVRSVLSIGRPRDVRRGMKVISAQVRPDVAALRD
                     DDVDVGSILPSV"
     gene            complement(1561..1869)
                     /locus_tag="VF058_04830"
     CDS             complement(1561..1869)
                     /locus_tag="VF058_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX6207667.1"
                     /translation="MEETRIAGGADIPEGEVRAFEVGDAEIAVANVGSELLAFSNVCT
                     HQQCPLDDGDLEDASIICACHGSQFDLRTGEVISPPATEPIEVYGVAVEGHDLVISGS
                     "
     gene            1954..3486
                     /gene="hutH"
                     /locus_tag="VF058_04835"
     CDS             1954..3486
                     /gene="hutH"
                     /locus_tag="VF058_04835"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX6207668.1"
                     /translation="MEPVLLGPDPLPAADVVAVARRDRAVAIDPGALDDVAAARSRIE
                     EHLGSGQAIYGITTGFGALAGVRVEQEDLDRLQRNLIRSHAAGAGEPLAREIVRAMLL
                     LRARALASGRSGVRPEVVERYVALLNEGITPAVPDRGSVGASGDLAQLAHVALAVMGE
                     GEVLADAGTEPSRRALARAGIEPLSLQAKEGLALINGTEGMLAVGVLAHHDAARLLRL
                     ADVTAAMSIESALGTDRPFAEDLMALRPHPGQAASAENLRRLLAGSEIVAGHRESEHA
                     VQDPYSMRCAPQVHGATRDAHGFAGEVFARELASVVDNPVILPDGRVESNGNFHGEPL
                     AQALDFLGIALTGLASIAERRTAWILGIGAERGLPPFLSKEPGLGSGYMLAQYTQAAL
                     VSECKGLAHPSSVDSIPTSGLQEDHVSMGWHGALRVREIVGRLRTVLAAEAMCGAQAL
                     DLRRPARPGEGTEAARAAIRERVPFLEHDREVAVDLRAVEEMLQDGSLLEAVEAAAGE
                     LR"
     gene            3483..>4953
                     /gene="hutU"
                     /locus_tag="VF058_04840"
     CDS             3483..>4953
                     /gene="hutU"
                     /locus_tag="VF058_04840"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005168430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX6207669.1"
                     /translation="MTREVRAPRGTGLSCKGWPQEAALRMLMNNLDPEVAERPEDLIV
                     YGGTGRAARSWDAFDAIVRSLRELEDDETLLIQSGKPVGVFRTHPWAPRVLISNAMLV
                     PKWADWETFRRLEREGLTMYGQMTAGSWIYIGTQGILQGTYETLAELASQRFQGTLSG
                     RIVLTAGLGGMGGAQPLAVTMNEGVALVVEVDPARIERRIKTRYLDRQTEDLDQAVRW
                     AEEARAEGEAVSIGLLGNAAETHPELLRRGFRPDVVTDQTSAHDPLEGYVPAGLSLEE
                     AAELRRTAPDDYVWRARTSMAAQVEAMIGFMDAGAVTFDYGNNLRAEARQGGAERALD
                     YPGFVPAFVRPMFCEGKGPFRWAALSGDPEDILTTDRAIAELFPQDERLHRWLRMAQE
                     RVAFQGLPARICWLGYGERARAGLRFNELVSSGELAAPIVIGRDHLDSGSVASPNRET
                     EGMADGSDAIADWPLLNGLVNACSGATWVSIHHGGGVGIG"
BASE COUNT          677 a         1844 c         1767 g          665 t
ORIGIN      
        1 ctggtctgcc gtgatcgact cggccagcga gcagccggct cggagatcgg ggatcagcac
       61 cgtccggtcc ggcgagagga tcttcgccgt ctcggccatg aagtggaccc cgcagaagac
      121 gatggtcgag gcgcccgtct cggcggcctt gcgcgaaagc tccagcgagt cccccacgac
      181 gtcggccacg tcctggatcc aggggagctc gtagttgtgg gccaggatca cggcgtcgcg
      241 ctcctcggcc agcgagcgca cggcgccctg gaacgcctcg aacgactcga gggccggatc
      301 gcgcagatcg ggctggacga tgctcatcgc atccccatga tacgggggct cagacgctcg
      361 ggaggatgga cccgacgtcc acgtcgtcgt cgcggagcgc cgccacgtcg ggacggacct
      421 gtgccgagat gaccttcatc ccccgcctga cgtcccgggg gcgaccgatc gacagcaccg
      481 accgcaccat cccgtccttc atgtagaagg ccgtgaagtc gcgatcctcg acggagcccc
      541 gcaccacgat ctcgtcccac gtcgcggcga acccgcccat ctgcaggttg acgtcgtact
      601 ggtccgacca gaaccagtgc gcgtcgtcga agggctcggg cgaccccagc atggccttgg
      661 ccgcgtgcgc tcccatcttc agggcgttgt cgaagtgctc cacgcggatc cggccgaaca
      721 cggggtggtc gtgggccgcc acgtcgccgg cggcgaacac cctctctgcc gaggcggcgc
      781 agcgtgcgtc gaccgcgatc ccgttgtcga tctcgagccc cgcggcctcc gcgagctcgg
      841 tgttcgggat gatgcccgcg ccgatcacca cggcgtcgcc ctcgagggca tcgccctcgt
      901 cggtctccac gatcaccgac tcgcccgtct cccgcacctc ggtcagcccc gtctccaggc
      961 gcaggtccac gccccgctcc cggtggatct cggcgagcac ccggcccatc ccctcaccca
     1021 gcacccggtt gagcggcgag gccgcccgct cgatcatcgt cacgtcggtg ccgagggccc
     1081 gggccgaggc cgcgacctcg gacccgatga agccggcgcc gacgacgagg aggtgcctcc
     1141 cgccacccag gtggcggcgc agccggtcgg cgtcctcgac cgtccggaga tagcagacgc
     1201 ggtcggaggg ctccgccccc aggatccggg cccggccgcc cgtggccagc agcagcacgc
     1261 ggtagccgac cgtggacccg tcctcgagct cgaccgtgcg atcggcgacg tccagacccg
     1321 tggcccggac cccgatccgc gcgtcgatcc cccactcctc gtaggaggcg tcctggtgga
     1381 ggggcagtgc ggcggcgtcg gcctctcccc tgaggtactc cttggagagc ggcgggcgct
     1441 cgtagggaag atgggactcg gcgccgatca gcacgatcgg accgtcctcg ccctcctcgc
     1501 gcagcgtctg caccgcggcc ccagccgcca gcccgccgcc gacgatgacg acaggatcgc
     1561 tcacgatccg gagatgacga ggtcgtggcc ctcgacggcg acgccgtaga cctcgatcgg
     1621 ctccgtggcg ggagggctga tcacctcgcc ggtccgcagg tcgaactggc tcccgtgaca
     1681 ggcgcagatg atcgacgcgt cctccagatc cccgtcgtcg agggggcact gctgatgcgt
     1741 gcagacgttg ctgaaggcga gcagctcgct ccccacgttg gccacggcga tctccgcatc
     1801 gcccacctcg aacgcgcgga cctccccctc ggggatgtcc gcgccgccgg cgatcctcgt
     1861 ctcctccatg cgaccccctt ggtcgtgcga ccggtcccat cctagggcac ggaccggatt
     1921 gcggtccgcg ggcggggggc gtgaaatgtg gccgtggaac ccgttctgct cggccccgac
     1981 ccgctccccg cggccgacgt cgtcgccgtg gcccgccggg accgggccgt cgcgatcgac
     2041 cccggcgcgc tggacgatgt ggccgcggcc cggtcccgga tcgaggagca cctgggcagc
     2101 gggcaggcga tctacggcat caccaccggg ttcggcgccc tggccggcgt gcgggtggag
     2161 caggaggatc tcgaccggct gcagcggaac ctgatccgga gccacgcggc cggcgccggc
     2221 gagcccctcg cccgagagat cgtgcgcgcc atgctcctcc tgcgggcgcg ggccctggcc
     2281 tcgggccgct ctggcgtccg tccagaggtc gtcgagcgct acgtcgccct cctcaacgag
     2341 gggatcaccc ccgccgtgcc cgaccgaggc tccgtggggg ccagcggaga cctggcccag
     2401 ctggcccacg tcgccctcgc cgtgatgggt gagggcgagg tgctggccga cgccggcacc
     2461 gagccctccc gccgggcgct ggcccgcgcg gggatcgagc cactctcgct ccaggccaag
     2521 gagggtctgg cgctgatcaa cggcaccgag gggatgctcg cggtgggcgt gcttgcgcac
     2581 cacgacgccg cgcggctcct gcgcctggcc gacgtcaccg cggcgatgtc gatagagagc
     2641 gcgctcggga ccgaccgccc cttcgccgag gacctgatgg ccctccggcc gcacccgggg
     2701 caggccgcct ccgcggagaa cctccggcgg ctcctggcgg ggagcgagat cgtggcgggc
     2761 caccgcgaga gcgagcacgc cgtccaggac ccctactcga tgcggtgcgc cccccaggtc
     2821 cacggcgcga cgcgggacgc ccacggcttc gccggcgagg tcttcgcccg ggagctcgct
     2881 tcggtggtcg acaaccccgt gatcctcccg gacgggcggg tcgagtcgaa cggcaacttc
     2941 cacggcgagc ccctggccca ggccctcgac ttcctgggga tcgccctgac cggcttggcc
     3001 agcatcgccg agcgccggac cgcctggatc ctcggcatcg gcgccgagcg cggcctcccc
     3061 ccgttcctct cgaaggagcc cggcctcggc tccggctaca tgctggccca gtacacgcag
     3121 gcggccctcg tgtccgagtg caaggggctg gcccatccgt cgagcgtgga ctcgatcccc
     3181 acctccgggc tgcaggagga tcacgtgagc atggggtggc acggcgccct gcgggtccgc
     3241 gagatcgtgg ggcgcctgag gaccgttctc gcggccgagg cgatgtgcgg cgcgcaggcg
     3301 ctcgatctcc gccgcccggc ccgccccgga gagggcaccg aggcggcccg ggcggcgatc
     3361 cgggagcgcg tccccttcct ggagcacgac agggaggtgg ccgtcgacct ccgcgcggtc
     3421 gaggagatgc tccaggacgg gtccctgctg gaagccgtgg aagcggccgc gggggagctg
     3481 cgatgacgcg cgaggttcgg gcgccgcgag gaaccgggct ctcgtgcaag ggctggccgc
     3541 aggaggccgc cctccggatg ctgatgaaca acctcgaccc cgaggtggcc gagcgccccg
     3601 aggacctcat cgtctatggg ggcacgggcc gggccgcccg ctcgtgggac gccttcgacg
     3661 cgatcgtccg gtcgctgcgc gagctcgagg acgacgagac cctcttgatc cagtccggga
     3721 agccggtcgg cgtcttccgg acgcacccgt gggcgccgcg ggtgctgatc tcgaacgcca
     3781 tgctggtgcc gaagtgggcc gactgggaga ccttccggcg cctcgagcgc gagggcctga
     3841 cgatgtacgg ccagatgacg gccggctcgt ggatctacat cgggacccag gggatcctcc
     3901 agggcaccta cgagaccctg gccgagctgg cttcccagcg attccagggg accctgagcg
     3961 gccggatcgt cctgaccgcg ggcctcgggg gcatgggcgg cgcccagccg ctcgcggtga
     4021 cgatgaacga gggcgtggcg ctcgtggtcg aggtcgaccc cgcgcggatc gagcgccgca
     4081 tcaagacgcg ctacctcgac cgccagaccg aggacctcga ccaggccgtg cgctgggccg
     4141 aggaagcccg agccgagggc gaggccgtga gcatcgggct cctcgggaac gcggccgaga
     4201 cccaccccga gctcctccgc cgcggcttcc gtcccgacgt cgtgaccgat cagacctccg
     4261 cccacgaccc gctcgagggc tacgtcccag cggggctctc gctcgaggag gcagccgagc
     4321 tccgccgcac ggcccccgac gactacgtct ggcgggcccg gacctcgatg gccgcgcagg
     4381 tcgaggcgat gatcggcttc atggacgcag gcgccgtcac gttcgactac gggaacaacc
     4441 tccgggccga ggcccggcag ggcggcgcgg agcgggccct cgactacccc gggttcgtgc
     4501 cggcgttcgt ccgaccgatg ttctgcgagg gcaaggggcc gttccgctgg gcggccctct
     4561 ccggtgatcc ggaggacatc ctcacgaccg accgcgccat cgccgagctc ttcccccagg
     4621 acgagcggct ccaccggtgg ctccggatgg cccaggagcg ggtggccttc caggggctgc
     4681 cggcgaggat ctgctggctc ggctacggcg agcgggcccg ggccggcctc cgcttcaacg
     4741 agctcgtgtc gtccggcgag ctggcggccc cgatcgtgat cgggcgagac cacctcgact
     4801 cgggcagcgt ggcatcgccg aaccgcgaga cggaggggat ggccgacggc tccgacgcga
     4861 tcgccgactg gccgctgctc aacggcctcg tcaacgcgtg ctcgggcgcg acctgggtca
     4921 gcatccacca cggcggtggt gtcggcatcg gtc
//
LOCUS       DASUTW010000147         2027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_564904, whole genome shotgun sequence.
ACCESSION   DASUTW010000147 DASUTW010000000
VERSION     DASUTW010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564904"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /gene="hisI"
                     /locus_tag="VF058_04845"
     CDS             <1..273
                     /gene="hisI"
                     /locus_tag="VF058_04845"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX6207670.1"
                     /translation="VVQDEATGDVLMVAWMNEEAVRATLDEGRTVFWSRSRGELWRKG
                     ETSGNVQHVREVRYDCDADTLLVRVHADGPACHTGERSCFYRRLEA"
     gene            276..1154
                     /gene="hisG"
                     /locus_tag="VF058_04850"
     CDS             276..1154
                     /gene="hisG"
                     /locus_tag="VF058_04850"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX6207671.1"
                     /translation="MLRLVIPKGSLEEQTLSLLEGADLRVRRGSSRDYHGRIEDERID
                     RVSLMRPQEIPRYIEEGFFDLGITGRDWVEETGADVEVLLELTYAKGGPGRPVKIVLA
                     VAADAPWEKAADLPDGTRISTEYPELTKRRFAELGIDVRVFESYGATEAKVPEIVDAI
                     VDVSETGGTLRAHGMKIIDELMESEVLLIANHRAWEDPDKRRAMEDVRTLIQGAMEAK
                     GRVLIKLNVTREKLDNVLEVLPAMKAPTVSSLAEEDEFAIETVVEKATVNALIPLLKA
                     HGATDILEIPISKIVP"
     gene            1151..1435
                     /locus_tag="VF058_04855"
     CDS             1151..1435
                     /locus_tag="VF058_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207672.1"
                     /translation="MKRVERRLLKVMDELLALDREQRLLEGELEMHRSLRDDAARDAA
                     VHETPVERGNAYETEKDVRTFERALAGLAARRAALEAKRDRLMTRLGRTP"
     gene            complement(1436..>2027)
                     /locus_tag="VF058_04860"
     CDS             complement(1436..>2027)
                     /locus_tag="VF058_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HEX6207673.1"
                     /translation="AAGPPEGRAHRGEPPSVALADRAAPLIEARLPEGVVADRAVRER
                     PGWRLAPMRPALRRARRAAASLARGGAVVATDVEAFFPSVSPEALERALLAVGVARGD
                     AAAAASMVAGWAEQGCPGLPVGPEASSILANAVLLPVDLAVAPARFLRWVDDYLLPAE
                     YPLERFDEALARVGLRRSERKTVSGIRGVWRAPYGR"
BASE COUNT          315 a          725 c          741 g          246 t
ORIGIN      
        1 gtcgtccagg acgaggccac gggcgacgtc ctgatggtgg cgtggatgaa cgaggaggcc
       61 gtacgggcca cgctcgacga gggccgcacg gtcttctggt cccgctcccg cggggagctc
      121 tggcgcaagg gcgagacgag cgggaacgtc cagcacgtcc gcgaggttcg ctacgattgc
      181 gacgccgaca cgctgctcgt gcgggtgcac gccgacggac cggcgtgcca caccggcgag
      241 cggtcgtgct tctaccggcg tctggaggcc tgaggatgct gaggctggtc atcccgaagg
      301 ggtcgctcga ggagcagacg ctctcgctgc tcgagggggc cgacctgcgc gtgcgtcgcg
      361 gctcctcccg cgactaccac ggccggatcg aggacgagcg catcgaccga gtctcgctga
      421 tgcgccccca ggagatcccc cgctacatcg aggagggctt cttcgatctg gggatcacgg
      481 gccgggactg ggtcgaggag accggggccg acgtcgaggt cctcctcgag ctgacctacg
      541 ccaagggagg ccccggccgg cccgtgaaga tcgtgctcgc ggtcgcggcc gacgcgccgt
      601 gggagaaggc cgcggacctc cccgacggca cgaggatctc cacggagtat cccgagctca
      661 ccaagcgccg cttcgccgag ctcggcatcg acgtccgcgt cttcgagtcc tacggggcga
      721 ccgaggccaa ggtccccgag atcgtggacg cgatcgtgga cgtctccgag accggcggca
      781 cgctccgggc ccacggcatg aagatcatcg acgagctgat ggagtccgag gtcctcctga
      841 tcgcgaacca ccgcgcctgg gaggaccccg acaagcgccg cgcgatggag gacgtccgca
      901 ccctgatcca gggggcgatg gaggccaagg gccgggtgct gatcaagctg aacgtcacga
      961 gggagaagct cgacaacgtg ctcgaggttt tgccggccat gaaggccccc accgtctcct
     1021 cgctggccga ggaggacgag ttcgcgattg agaccgtggt cgagaaggcc acggtcaacg
     1081 cgctcatccc gctgctgaag gcccacgggg cgaccgacat cctcgagatc cccatctcca
     1141 agatcgtgcc gtgaagcgcg tggagcgacg gctgctgaag gtgatggacg agctcctggc
     1201 gctcgatcgt gagcagcggc tgctcgaggg cgagctcgag atgcaccgga gccttcggga
     1261 cgacgcggcc cgcgacgccg ccgtccacga gacccccgtg gagcgcggga acgcctacga
     1321 gaccgagaag gacgtccgga ccttcgagcg cgccctggcg gggctcgcgg cccggcgcgc
     1381 cgcgctcgag gcgaagcgtg atcgcctcat gacgcgcctg ggcaggacgc cctgatcagc
     1441 ggccgtacgg ggcccgccac acgccgcgga tcccgctcac cgtcttccgc tcggatcgcc
     1501 tgagccccac ccgcgcgagc gcttcgtcga accgctccag cgggtactcc gcggggagga
     1561 ggtagtcgtc cacccaccgc aggaaccggg ccggtgccac ggcaaggtcc acggggagga
     1621 ggaccgcatt ggccaggatc gaggaggctt cggggccgac ggggaggccc gggcagccct
     1681 gctcggccca tcccgcgacc atcgacgccg ccgcggccgc gtcgccgcgc gcgaccccca
     1741 cggctaggag ggcccgctcg agcgcctccg ggctcacgga cgggaagaag gcttcgacgt
     1801 cggtggcgac caccgctccc ccgcgcgcga gcgacgcggc cgcccggcgg gccctccgga
     1861 gcgccgggcg catcggggcg agccgccaac cggggcgctc gcggaccgcg cggtccgcaa
     1921 cgaccccctc gggtagcctc gcctcgatca gcggagccgc gcggtccgcc agcgccacgg
     1981 atggcggctc gccccgatgc gcccggccct ccggagggcc cgccgcg
//
LOCUS       DASUTW010000148         2051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1471455, whole genome shotgun sequence.
ACCESSION   DASUTW010000148 DASUTW010000000
VERSION     DASUTW010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471455"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2051)
                     /locus_tag="VF058_04865"
     CDS             complement(<1..>2051)
                     /locus_tag="VF058_04865"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6207674.1"
                     /translation="GQVLDLPAAALAFTEDEVAELLRAVLGEEPGDLAGSLHRLTEGW
                     PAAVRLGAEVAASTDPEQRTSALEALSRPGSPVFDYFAREVLGAEEPHVLGFLRTAAL
                     LDAFSPELCAAIGVEDAERTIADLSSRGLFFEPRGEGWFELSPLIRGVVERSGPGGGE
                     AAALLTSAAKWLLEAGRIEDALGALLRAEDLEGVAAALEEHGEGLIAGGSVEAVIRSI
                     EPLPADLRSPALDRLEGEARQIRGDWDRALACFLRAAPEGGPLDAGLAWRMGLIHHLR
                     GDLGAALDMYERGRVDREDRDVALLLAWSAAAHWLRGDLERSRETAGRARDVAAAVGD
                     ERAEAAAFTTLAMVAALEGDRRANDAYYLRALDAAERARDVLQIVRIRTNRASRFSEE
                     GDHEDALRELDLAVGLAELTGFANFHALGVHNRGQARLGLGRLEEARTDFESALALYQ
                     RMGSRMVAYPLAGLADISRTRGDLAQARAAYEEAVAVAEEAGDLQGLVPAMAGLARVL
                     AAEDPEAARKTAERAVELGRGMGYVQAQLALGWVALASGERDPARGAAQAASEAARER
                     RDRPGLAEALTLAAMTDPDEPWVPTALEEAAGIWREVGNPLEEAIVGIVASGLGGPTE
                     AGPSAERARRRLRAYGVRLPAAAQPAGPLHLLSSEEPSLVIQALGGFRVLRGGRAVPA
                     S"
BASE COUNT          250 a          861 c          704 g          236 t
ORIGIN      
        1 cggaggcggg gacggcccgc cccccgcgca gcacgcggaa cccgcccagc gcctggatca
       61 cgagcgaggg ctcctcgctc gacagcaggt ggagcggccc ggccggctgg gcggccgcgg
      121 gcaggcgcac gccgtaggcc ctgaggcgtc gcctggccct ctccgcggac ggtcccgcct
      181 cggtcgggcc gcccaggccc gacgcgacga tccccacgat cgcctcctcc agggggttgc
      241 ccacctcccg ccagatcccc gcggcctcct ccagcgccgt cggcacccag ggctcgtccg
      301 gatccgtcat cgccgccagg gtgagggcct cggcgagccc gggacgatcg cggcgctcgc
      361 gggccgcctc ggacgccgcc tgggccgccc cccgcgccgg gtcccgctcg ccggacgcca
      421 gcgccaccca gcccagcgcg agctgcgcct gcacgtatcc catgccccga cccagctcca
      481 cggcccgctc ggccgtcttt cgggccgcct ccggatcctc cgcagccagc acgcgggcca
      541 gcccggccat cgccgggacg aggccctgga ggtcccccgc ctcctcggcc acggccaccg
      601 cctcctcgta cgccgcccgg gcctgcgcca gatcccctcg ggtccgggag atgtcggcca
      661 ggcccgccag tgggtaggcc accatccgag agcccatccg ctggtagagc gcgagggccg
      721 actcgaagtc cgtccgtgcc tcctccagcc gacccagtcc cagacgggcc tggcccctgt
      781 tgtgcacgcc gagcgcgtgg aagttggcga atccggtcag ctcggccagc cccaccgcca
      841 ggtcgagctc gcggagggcg tcctcgtggt cgccctcctc gctgaaccga gaggctcgat
      901 tcgtacggat cctgacgatc tggaggacgt ctcgggctcg ctcggcggcg tccagcgcac
      961 ggaggtagta ggcgtcgttg gcgcggcggt ctccctccag cgcggccacc atggccaggg
     1021 tggtgaacgc ggccgcctcg gccctctcgt ctcccaccgc cgcggccacg tctcgggccc
     1081 tcccggccgt ctcgcgggac cgctccaggt ccccccgcag ccagtgggcg gccgcggacc
     1141 aggccaggag gagtgcgacg tcccgatcct cacgatccac ccgcccccgc tcgtacatgt
     1201 ccagtgcggc cccgaggtcg ccccggaggt gatggatcag gcccatccgc caggccagcc
     1261 cggcgtcgag gggccccccc tccggcgccg cccggaggaa gcaggccagg gcgcggtccc
     1321 agtccccgcg gatctgccgg gcctccccct cgaggcgatc cagggcgggc gagcgcagat
     1381 cggcgggcag cggctcgatc gatcggatca cggcctccac cgaccctccc gcgatcagcc
     1441 cctcgccgtg ctcctccagc gccgcggcca ccccctcgag atcctccgcc cgcaggaggg
     1501 ctcccagcgc gtcctcgatc cgccccgcct ccagcagcca cttggcggcc gaggtcagca
     1561 gcgcggcggc ctcccctccc cccgggccgg agcgctcgac caccccccgg atcagcggcg
     1621 acagctcgaa ccagccctcc cctcgcggct cgaagaagag gccccgggag gacaggtcgg
     1681 cgatcgtcct ctccgcgtcc tccaccccga tcgccgcgca gagctcgggg ctgaaggcat
     1741 cgaggagggc ggccgtcctg aggaacccca ggacgtgagg ctcctccgcg cccagcacct
     1801 cgcgggcgaa gtagtcgaag acggggctgc ccggccggct gagggcctcc agcgcgctgg
     1861 tccgctgctc ggggtcggtg gaggcggcca cctccgcccc gagccggacg gccgccggcc
     1921 acccctcggt gagccggtgg agcgagccgg ccaggtcgcc cggctcctcc cctaggacgg
     1981 cccggagcag ctcggcgacc tcgtcctcgg tgaaggcgag cgcggccgcg ggcaggtcga
     2041 ggacctgccc g
//
LOCUS       DASUTW010000149         5759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1082535, whole genome shotgun sequence.
ACCESSION   DASUTW010000149 DASUTW010000000
VERSION     DASUTW010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082535"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..535)
                     /locus_tag="VF058_04870"
     CDS             complement(35..535)
                     /locus_tag="VF058_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009358745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207675.1"
                     /translation="MATIAHERTGAPEAETRLRIGAWLMGLAAVGFIGYAVIFFILNF
                     TDQFLELGIGAEQVDVGKAEIEAFSPSLLHYVGHLHIAVSGFIASTGLAVLFLVGFGV
                     RRGELWAWVGAVAAPVLGLLVVLPAHYLYGLDTLGHLGLIYLATGIFVGGALVALRGI
                     LAQRSV"
     gene            653..1252
                     /locus_tag="VF058_04875"
     CDS             653..1252
                     /locus_tag="VF058_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6207676.1"
                     /translation="MDRGDMVDVRDRTREAFQRIAVTELPALYSLARRLVREGAEDLV
                     QEALLRAYRSFDALREEASAGPWLKTILVNVFRDRLRKEARSVDEIPVEEVEDFSLYG
                     TLIDEDPLPYSDTLHLDFLRAFAREDVREVLMRLPEIYRAPLVLRYMDGFATKEIARM
                     LGVPLGTALARLHRGRKRFEKEMWDYAEEMGILLKGGTR"
     gene            1249..1491
                     /locus_tag="VF058_04880"
     CDS             1249..1491
                     /locus_tag="VF058_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6207677.1"
                     /translation="MSDVQRTIPCSEAVRALWDYLDQAIAPEDQKRVEQHLAFCRRCC
                     GEMEFATELRGFLASQQREELPAEVRARLQRFVDEL"
     gene            1502..2080
                     /locus_tag="VF058_04885"
     CDS             1502..2080
                     /locus_tag="VF058_04885"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6207678.1"
                     /translation="MDRDLMYQDEIKRTVREAYAAIPTGAGETVARHLYSEEELAQVP
                     PGAVGWALGVGNPVRYAMLQPGETVLDVGSGGGIDTILAAHRVGPEGRAMGLDIIDEM
                     IRRGRRHAQEAGVGEWAEFLEGEMEDVPLPDASIDVVISNGVLNLSARKSRALSEIHR
                     VLRPGGRISIADLTVEGDLPPEVANDQSAWAG"
     gene            2093..2359
                     /locus_tag="VF058_04890"
     CDS             2093..2359
                     /locus_tag="VF058_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207679.1"
                     /translation="MAERVFAGKLERAGFADVWLGGHRPFGIDEAAHYPLFTAEVIEV
                     MRRTIPSDRHDHVATGVIVKARKPVVASGEGAPWTTARRTSEEG"
     gene            complement(2740..2823)
                     /locus_tag="VF058_04895"
     tRNA            complement(2740..2823)
                     /locus_tag="VF058_04895"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2787..2789),aa:Leu,seq:cag)
     gene            2914..3615
                     /locus_tag="VF058_04900"
     CDS             2914..3615
                     /locus_tag="VF058_04900"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HEX6207680.1"
                     /translation="MGILRDFEGRLERIVEGFFSKAFRSGLQPVELAKRILREMDAGK
                     TVGVSGVLVPNRFVVVLSEEDRERLTGMESALSRELETVVVDGAREREFTLVARPEVV
                     FETAERLKRGDFRIQAEISEATGPPPADVGPRPGGGGPLLALVEGGDALREYPITSDR
                     MTIGRLAGSEIELHDPGASRKHAEIRRRGDTVTVVDLGSTNGTLVNDRTITDHVLRDG
                     DRIRIGRTTLEFRSG"
     gene            3619..4068
                     /locus_tag="VF058_04905"
     CDS             3619..4068
                     /locus_tag="VF058_04905"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX6207681.1"
                     /translation="MPPFVLTVLKILLLLLLYFFLYRAVRSIARDLYPRTRERRREAR
                     PARTRRGRGRPGRVVVLGPEGKKVGTYRLQGAMQIGRDAACEIRPPDTYISQVHARLA
                     PRGDGWVVEDMGSTNGTYLNQRKVQVPTRIAPGDRIRLGKTVLEVRK"
     gene            4077..5174
                     /locus_tag="VF058_04910"
     CDS             4077..5174
                     /locus_tag="VF058_04910"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX6207682.1"
                     /translation="MGATTDVGVVREQNEDAYLVRAPIYAVADGMGGHQGGEVASRLA
                     LETLDRRSREDHEVDLRAAVKDANRVVLDRASGDTELAGMGTTLTLLRIEGPRVRLAH
                     VGDSRAYLLRDGDLRQLTDDHTLVNKMVKEGKLTTSEARIHPHRSILTRALGVDGDVE
                     VDVFTVDVDLGDRILLCSDGLTAIVSDEVIRGVLTSQGDPQAACDELVAMANRGGGPD
                     NITVVVLDFAEGQEGEEEPSTPAAAETPAPTPPPERRRRRPSLRGLLLWILLPLIILV
                     GGFLLARAYVNSQWFVGVHEGSVALYRGIPAEVLGFELFTLVERTDLPAAEAAALQPY
                     QDLGEGITADSEEDARDVIEQIEEDLEPPIP"
     gene            5171..>5759
                     /locus_tag="VF058_04915"
     CDS             5171..>5759
                     /locus_tag="VF058_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX6207683.1"
                     /translation="MSTPAPELIARARRNTELGLLILAVLLAVGAYALVGLGLEGRVP
                     AGVAGYGVLLAAGYVGAHLVVRYLAPHADPVFLPAATLLTGLGFAVIWRLDQDLAVAQ
                     TSWLLFGLLLFAATLVVIRDHRQLDAYTYTIGLLGIALLLLPILPGIGREVRGARLWV
                     EVGPLQFQPAEIGKVLIVIFLASYLNARKELLAVAT"
BASE COUNT          881 a         1980 c         2078 g          820 t
ORIGIN      
        1 gacgtgcgga ccgcacgtcg ccgccgcccc tcggtcagac cgacctctgg gccaggatcc
       61 cgcgaagagc caccagcgcg ccccccacga agatccccgt cgccaaatag ataaggccca
      121 ggtgccccag cgtgtccagt ccgtagaggt agtgcgcggg aaggaccacc agcaggccga
      181 ggacgggcgc ggccaccgca ccgacccagg cccagagctc gccccgacgc acgccgaacc
      241 ccacgaggaa caggaccgcg agcccggtgg atgcgatgaa gcccgacacc gcgatgtgca
      301 ggtgccccac gtagtgcagg agcgacgggc tgaaggcctc gatctccgcc ttccccacgt
      361 cgacctgctc cgcgccgatc ccgagctcca ggaactggtc ggtgaagttc aggatgaaga
      421 agatcaccgc gtagccgatg aaccctacgg cggccagccc catcagccag gctccgatcc
      481 ggagccgggt ctccgcctcc ggcgctcccg tccgctcgtg tgcaatcgtc gccatgcgcc
      541 tcgcctcctt tcacccagaa gggacgcggc gcctcgcccc cacattgcag ccccccgcgg
      601 atgcaataca ttcgtgtcgg ggaccgtcct tcgtgggtag ggtagccgcc gcgtggatcg
      661 aggagacatg gtcgacgtca gggaccggac cagggaggcg ttccagagga tcgccgtcac
      721 cgagcttccc gctctctact cgctcgccag gcggctcgtg cgggaggggg cggaggacct
      781 ggtccaggag gccctcctgc gggcctaccg ctccttcgac gcgctcaggg aagaagcctc
      841 ggcgggcccc tggctgaaga cgatcctggt gaatgtcttc cgggatcggc tgcggaagga
      901 ggcgcggtcg gtggacgaga tccccgtgga ggaggtcgag gacttctcgc tgtacggcac
      961 gctgatagac gaggaccccc tgccctactc ggacaccctg cacctggact tcctgcgggc
     1021 gttcgccagg gaagacgtcc gtgaggtgct gatgcgcctc ccggagatct accgggcgcc
     1081 cctggtcctc cgatacatgg acgggttcgc cacgaaggag atcgcccgga tgctgggcgt
     1141 ccccctgggg accgcgctcg cgcggctcca ccgcggccgg aagcggttcg agaaagagat
     1201 gtgggactac gcggaggaga tgggaatcct ccttaaggga gggacgcgat gagcgacgtc
     1261 cagcggacga tcccctgttc cgaggcggtc agggcgctgt gggactacct cgaccaggcg
     1321 atcgcccccg aggatcagaa gagggtggag cagcacctgg cgttctgtcg ccggtgctgc
     1381 ggtgagatgg agttcgcgac ggagctcagg ggattcctcg cctcccagca gagggaggag
     1441 ctccccgcgg aggtccgggc gcggctccag cggttcgtgg acgagctcta ggggtgtcgt
     1501 cgtggatcga gacctgatgt accaggacga gatcaagaga acggtgcgcg aggcctacgc
     1561 ggcgatccct accggagccg gcgagaccgt ggcccggcac ctctattccg aggaggagct
     1621 cgctcaggtg cccccgggcg cggtcggctg ggctctgggg gtggggaacc ccgtccggta
     1681 cgcgatgctc cagcccggcg agaccgtgct cgacgtcggt tcggggggcg ggatcgacac
     1741 gatcctggcg gcccatcggg tagggccgga ggggcgggcc atggggctgg acatcatcga
     1801 cgagatgatc cggcgggggc gacgccacgc tcaggaggcc ggcgtcgggg agtgggcgga
     1861 gttcctggag ggcgagatgg aggacgtccc cctccccgat gcctccatcg acgtcgtgat
     1921 ctccaacggg gtcctcaacc tctccgcccg caagagccgg gccctgtccg agatccaccg
     1981 cgtgctccgc cccgggggcc ggatctccat cgccgatctc accgtggagg gcgacctgcc
     2041 cccggaggtg gcgaacgatc agagcgcctg ggccgggtga gtgtccggcg ccatggcgga
     2101 gcgtgtcttc gccgggaagc tcgagagagc ggggttcgcc gacgtctggc tggggggaca
     2161 tcggcccttc gggatcgacg aggccgccca ctatcccctg ttcaccgcgg aggtcatcga
     2221 ggtcatgcgg cgcacgatcc cgagcgaccg gcacgaccac gtcgccaccg gcgtgatcgt
     2281 gaaggcccgg aagccggtcg tcgcctccgg tgagggggcc ccttggacga cggctcgacg
     2341 aacgagcgag gaaggatgac gcgatccgtc gatctctacg acgcgagcta cgggaacctt
     2401 cgatgctcgg gagcgttcgt cgcgagacct acgggatgag gagatcggca tccctgcatc
     2461 gcctcgggcg atcctcttct cgctccccga ggaggacgag cgactcgtga acgaagccgg
     2521 cttctctctc cgtcacgagg cgtgacggcg agcgtcgtcg gggtcgccgg ccggtggccg
     2581 gaggcgcggg aacggcatcg agaggccctc gtggccgggg gaggaacctt cgagggcgcg
     2641 cggcacttcc tccacgccgc gaggacgctg gcggaggaag gacggcttct cgcggcacgt
     2701 cttcctcgcc gagagggggg cgtgatgctg gctagcgagt gggcgagggg ggagtcgaac
     2761 ccccatgggc ttgcgcccac aggatcctga atcctgcgcg tctaccagtt ccgccacccg
     2821 cccgggtgat ccgtagggac gggcccatgg tactcgaccg gccgcccccc ggccgggtgt
     2881 agggacaggg cgagcgcggt accctttgcc cggatgggga tcctgcgaga cttcgagggc
     2941 aggctggaga ggatcgtcga gggcttcttc tcgaaggcct tccgatcggg cctccagccg
     3001 gtcgagctgg ccaagcgcat ccttcgcgag atggacgcgg ggaagacggt cggcgtcagc
     3061 ggcgtgctcg tccccaaccg cttcgtggtc gtcctgtccg aggaggaccg ggagcgcctc
     3121 accgggatgg agtcggccct gtcccgagag ctcgagaccg tggtggtgga cggcgccagg
     3181 gagcgcgagt tcacgctcgt ggcccgaccc gaggtcgtct tcgagacggc ggagcgcctg
     3241 aagcggggcg acttccggat ccaggccgag atctcggagg cgaccggccc gcccccggcc
     3301 gacgtgggcc cccggcccgg gggaggcggc cctctgctcg cgctggtcga ggggggcgac
     3361 gccctccgcg agtacccgat cacgagcgac cggatgacga tcgggcgcct ggcgggctcc
     3421 gagatcgagc tgcacgaccc gggtgcgagc aggaagcacg ccgagatccg gcgccgcggc
     3481 gacacggtca ccgtcgtcga cctcggatcg acgaacggca ccctggtcaa cgaccggacc
     3541 atcaccgacc acgtcctccg cgacggcgac cggatcagga tcggccgcac gacgctggag
     3601 ttcaggagcg ggtagcccgt gcctccgttc gtactcacgg tcctcaagat cctcctcctg
     3661 ctgctcctct acttcttcct ctaccgggcc gtgcgatcca tcgcccgcga cctctacccg
     3721 cggacgcggg agcggcgccg cgaggctcgt ccggcccgga cccgaagggg acgggggcgg
     3781 ccgggccgcg tggtcgtgct ggggccggag gggaagaagg tggggaccta ccggctccag
     3841 ggggcgatgc agatcgggag ggacgccgcc tgcgagatcc gcccgcccga cacgtacatc
     3901 tcccaggtcc acgcgcgttt ggctccgcgc ggcgacggct gggtggtcga ggacatgggc
     3961 tccacgaacg gcacctatct aaaccagcgg aaggtgcagg tgccgacgcg catcgcgccc
     4021 ggcgaccgca tccgcttggg gaagacggtc ctggaggtga ggaagtgagg gtccgggtgg
     4081 gcgccaccac ggacgtcggg gtcgttcgcg agcagaacga ggacgcctac ctggtccgag
     4141 cgccgatcta cgcggtcgcc gacggtatgg gcgggcatca gggcggggag gtggcgtcgc
     4201 gcctcgccct ggagaccctc gacaggagga gccgcgagga ccacgaggtg gaccttcggg
     4261 ccgcggtcaa ggacgccaac agggtcgtcc tcgatcgcgc gtcgggcgac accgagctcg
     4321 cgggcatggg gaccaccctc acgctcctca ggatcgaggg cccccgcgtg cgcctggccc
     4381 acgtgggcga ctcgcgcgcg tacctcctcc gggacgggga cctcaggcag ctgaccgacg
     4441 accacaccct cgtcaacaag atggtcaagg aggggaagct caccacgagc gaggccagga
     4501 tccaccccca tcgcagcatc ctcaccaggg cgctcggggt cgacggcgac gtcgaggtgg
     4561 acgtcttcac ggtggacgtg gacctcggcg accgcatcct gctctgcagc gacgggctca
     4621 cggcgatcgt gagcgacgag gtgatccgcg gggtcctcac ctcgcagggc gacccccagg
     4681 ctgcctgcga cgagctcgtt gccatggcga atcgcggggg cggccccgac aacatcaccg
     4741 tcgtggtcct ggacttcgcg gagggccagg agggggagga ggagccctcg accccagccg
     4801 cggcggagac gccggccccc acccctcccc ccgagcgccg ccgccgccga ccgtccctcc
     4861 gagggctcct gctgtggatt ctcctccccc tgatcatcct ggtcggcggc ttcctcctgg
     4921 cccgcgcgta cgtcaactcg cagtggttcg tgggggtcca cgagggcagc gtcgccctgt
     4981 accgaggcat ccccgcggag gtcctcggct tcgagctctt cacgctcgtg gagcggaccg
     5041 accttcccgc ggcggaggcc gcagcgctgc agccgtacca ggatctcggg gaggggatca
     5101 ccgccgacag cgaggaggac gcccgcgacg tgatcgagca gatcgaggag gacctggagc
     5161 cgccgatccc atgagcacgc ccgcccccga gctcatcgcc cgggcccgcc gcaacaccga
     5221 gctgggcctc ctcatcctcg ccgtcctgct ggcggtgggg gcttacgcgc tcgtgggcct
     5281 gggcctggag ggccgggtcc cggccggcgt ggccggctac ggcgtgctcc tcgccgccgg
     5341 ctacgtgggg gcgcatctgg tggtccgata cctcgccccc cacgccgacc ccgtgttcct
     5401 ccctgcggcc acgctcctta ccgggctggg gttcgccgtg atctggaggc tcgaccagga
     5461 cctcgccgtg gcccagacct cctggctgct gttcgggctc ctgctcttcg cggccaccct
     5521 ggtcgtgatc cgcgaccacc ggcagctgga cgcctacacg tacacgatcg gcctgctagg
     5581 gatcgccctc ctcctgctgc cgatcctccc ggggatcgga agggaggtcc gaggcgccag
     5641 gctctgggtg gaggtggggc cgctgcagtt ccagcccgcg gagatcggga aggtcttgat
     5701 cgtgatcttc ctcgcctcgt acctcaacgc gcggaaggag ctgctggccg tggccacga
//
LOCUS       DASUTW010000150         8934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_825080, whole genome shotgun sequence.
ACCESSION   DASUTW010000150 DASUTW010000000
VERSION     DASUTW010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8934
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825080"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..967
                     /locus_tag="VF058_04920"
     CDS             74..967
                     /locus_tag="VF058_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6207684.1"
                     /translation="MRKIAAVLMISGLVLAACAPEEEDETPEAAPTTSPTVDECATEN
                     LTLVTPGQLTIGTDNPAFPPWFEGGTPEGSSWEINDPSTGEGFESAVAYAAAEELGFG
                     EDEVEWVEVPFNQAFAPGPKDFDFDINQVSYRPRRDEAVDFSDSYYDVNQALVAVAGT
                     PIAEATTLEELKQYTLGAPVGTTSFEYIDQNIQPDSDPRVYDTLNDSISGLENEQVDG
                     IVVDLPTAFFIVAVQLDNGTIVGQFPTVGQQEYFGMVFEEGNPLRDCVNQALDTLRED
                     GTLASIQQEWLADKTDAPVLE"
     gene            984..1865
                     /locus_tag="VF058_04925"
     CDS             984..1865
                     /locus_tag="VF058_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX6207685.1"
                     /translation="MSVTTPPGSPAPPLPPGDRRSRILGGRHWEGRYAVPIAIASTAI
                     FFTVLVLLIVNSPGWPEVRRSFFNGEVFRDSFPDITAKFLKNVGAFLIAEVFILVLAL
                     FIAVLRSLPGPVFFPIRTLALGYTDLFRGIPTILVIYILGFGAPALQLQGVPKDPFFW
                     GIVALVLVYSAYVAEVYRAGIESVHPSQVAAARSLGLTRWQGLRFVVLPQAVRRVVPP
                     LLNDFIGLQKDTALLALIGVLETFRQSQVDVAGSFNYTPYLATAMLFVLITIPLARFT
                     DWLIARERRRHQAGSAL"
     gene            1862..2608
                     /locus_tag="VF058_04930"
     CDS             1862..2608
                     /locus_tag="VF058_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX6207686.1"
                     /translation="MTRALTLEGVHKSFGKVEVLRGVDLAVDQHEVIALIGASGSGKS
                     TLLRCVNLLEPVDAGRILLNEDEITLRGVNENEVRKRIGIVFQAFNLFPHMTVLRNVT
                     LGPIEALGLSREEAEARAEELLARFGLLDKRDEYPDRLSGGQQQRAAIVRAMAMQPDL
                     LLLDEVTSALDPELIAEVLDVIRELAEAGMTMILATHEMGFAKDIAGRVSFLDEGVIL
                     EEGPPDRMFAEPEHPRTRRFLQRIIAAGRL"
     gene            2624..4660
                     /gene="ligA"
                     /locus_tag="VF058_04935"
     CDS             2624..4660
                     /gene="ligA"
                     /locus_tag="VF058_04935"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX6207687.1"
                     /translation="MSEDRREPEERRRSEVRLRIEELREQIRHHQYRYYVEDDPEVSD
                     AEYDELVRELEKLEVEFPEFVTPDSPTQRVGGQVSELFAPVEHRTPMLSLDNAFTWEE
                     LSEWGRRVERVVGRAGDFVCELKIDGVAVALTYEDGRYVRGATRGDGYTGEDITANIR
                     TVGGVPMRLRGEHSGVLEVRGEVYLPVKAFEEMNRELQEAGGRAFANPRNAAAGSLRQ
                     KDPGVTARRPLRLWVHGLGWADGRRFSRHSEALDHLREAGLPVNPDNALLDTLEDVFA
                     FCERWQERRHDVDYEIDGVVVKVDQISLQEELGATAKAPRWAIAYKFPPEERTTLLRG
                     IFTHTGRTGKVTPFAQLEPVFVGGVTVTTATLHNESEVARKDVRPGDHVIVRRAGDVI
                     PEVVGPVMQKRKRGARRWKFPKECPSCGTPLVREEGEADWRCPNREGCPSQNIEWLFH
                     FAGRGAMDIEHLGYKTGMALMDRGWVTDPADVYFLTEEQLAELPGFKEKSIGNLLGAI
                     EDSKDRPIWRLLVGLNVRHVGTTVARMLTRAFPSIVALSKASAEDIEAVEGIGPEIAR
                     SVHEWFRRPESKRLLKKLKKAGVRMEDPEPEGPAPDGPLVGKRVVLTGGLESMSRDEA
                     TKASEAAGARVTSSVSKSTDFVVAGTDPGSKYDKAVSLGVEIIDEQEFRRRLGG"
     gene            5001..5342
                     /locus_tag="VF058_04940"
     CDS             5001..5342
                     /locus_tag="VF058_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207688.1"
                     /translation="MEALGERADQDAADPPEREVAQELVLVAVQWACGRPDRWLGTRA
                     MLGDEIGPTGVEESDEGIGTGDLVAPLDACDVGLVGAGAAGQLGLRETGEDAVPSDGV
                     ADIHGVVAMMG"
     gene            5685..5978
                     /gene="gatC"
                     /locus_tag="VF058_04945"
     CDS             5685..5978
                     /gene="gatC"
                     /locus_tag="VF058_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX6207689.1"
                     /translation="MPVEIDIEHVARLARLALTPEELERFREQLGVILEHAERVTEVA
                     AEAVPATAHPIPMANVFRPDEPRDGLGRDEALANAPDREGDFFKVPPIVEEPE"
     gene            5992..7467
                     /gene="gatA"
                     /locus_tag="VF058_04950"
     CDS             5992..7467
                     /gene="gatA"
                     /locus_tag="VF058_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX6207690.1"
                     /translation="MTELCDLTGHELAGKLRAGEVSAVDGVESSLSRIEATEPSLHAF
                     LARSADLAREQARAVDDRLAAGDDLPPVAGIPLALKDVLTTRGITTTCGSRILETYVP
                     PYDSTVWARLREAGSVLVGKTNCDEFAMGSSNENSAFGPVHNAWDLDRVPGGSSGGSA
                     AAVAAGQAVWALGTDTGGSVRQPAALSGVVGMKPTYGRPSRYGLIAFASSLDTVSTFT
                     RDVRDAATLLSAIVGHDPLDATSIQRPAEDYTEGLEQGVDGLRIGVLRDWLGEGVEPG
                     VRDRVEEAIQRLEGLGAKIGEATLPHADYALSAYYLIAPAECSSNLARYDGVRYGHRA
                     EGANVVEMMMRTRGEGFGDEVKRRIMLGTYALSAGYYEAYYGQAQKVRTLVLRDFEAA
                     WQEFDLLLSPTSPTVAFPLGAKADDPLAMYLSDVYTIPANLAGVPAVSVPCGLDDQGL
                     PVGLQLMGRALDEATVLRAAYAFEQDLGFDARPPLLEGIAS"
     gene            7464..8912
                     /gene="gatB"
                     /locus_tag="VF058_04955"
     CDS             7464..8912
                     /gene="gatB"
                     /locus_tag="VF058_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX6207691.1"
                     /translation="MTAYETVIGLECHVELSTKTKMFCSCRNEFGAEPNTLVCPICLG
                     HPGTLPVPNERAVEHTITIGLALGSEIAPTSLFHRKNYFYPDMPKNYQISQYDLPLCV
                     GGALEVEVDGEPRRVGITRVHLEEDTGKTTHAGATGRIAGADYALEDFNRAGVPLVEV
                     VSEPDLRSPAEARAYLVALRGLLRSLGVSDVKMEEGSLRCDANVSVRPEGHEELGTKV
                     EIKNMNSIRSVERALAFEAERQRAALDAGETLVQETRHWDEDQGETSSMRSKEEAFDY
                     RYFPDPDLSPLEPEAERVDRLRSELPELPAARRARLESELGVPAAQAAQVVEQGWDRF
                     LREAVAAGADAKGAANWMTGDLAALLNEAGIELGQAKVSPKHLADLVRLVADGTISTA
                     GAKQVLAEMFASGADAEAVVEERGLAQISDESALGAIVDEVIADNPDPVEQFRGGKEG
                     VIGFLVGQVMQRSKGAANPQEAQRLLRERLAR"
BASE COUNT         1342 a         3012 c         3293 g         1287 t
ORIGIN      
        1 cttctctcgg ggtgctacat tgcgccatcg gggaccggct caagtggccg gcaggaagag
       61 ggggagacgc tcgatgcgga agatcgccgc cgtcttgatg atctccgggc tcgtgctcgc
      121 cgcgtgcgcg cccgaggagg aagacgagac cccggaggcc gctccgacga cctcgcccac
      181 ggtggacgag tgcgccaccg agaacctcac cctcgtcacc ccgggccagc tcacgatcgg
      241 gaccgacaac cccgccttcc cgccctggtt cgagggtggg acgcccgagg gatcctcctg
      301 ggagatcaac gacccctcga ccggcgaggg cttcgagagc gcggtggcct acgcggccgc
      361 ggaggaactc ggcttcggcg aggacgaggt cgagtgggtc gaggtcccct tcaaccaggc
      421 gttcgcgccc ggaccgaagg acttcgactt cgacatcaac caggtctcgt accggcctcg
      481 gcgcgacgag gcggtggact tcagcgactc gtactacgac gtgaaccagg cgctggtggc
      541 ggtggccggt actccgatcg ctgaggccac cacgctcgag gagctgaagc agtacacgct
      601 cggcgccccg gtggggacca cgagcttcga gtacatcgat cagaacatcc agcccgacag
      661 cgacccacgc gtctacgaca cgctcaacga ctcgatcagc ggcctggaga acgagcaggt
      721 ggacgggatc gtggtggacc tccccacggc gttcttcatc gtggccgtcc agctcgacaa
      781 cgggacgatc gtggggcagt tcccgacggt ggggcagcag gagtacttcg ggatggtctt
      841 cgaggagggc aacccgctcc gggactgcgt gaaccaggcc ctggacacgc tccgcgagga
      901 cgggacgctg gcctcgatcc agcaggagtg gctggccgac aagaccgacg cgccggtcct
      961 cgagtaggcc tccggcgggg cagatgagcg tcacgacccc gccggggagc cccgccccac
     1021 ctctcccgcc gggcgatcgc cgctcgagga tcctcggggg gcgtcactgg gagggtcgat
     1081 acgccgtccc gatcgcgatc gcaagcaccg cgatcttctt cacggtgctc gtcctcctga
     1141 tcgtgaactc gcccgggtgg cccgaggtca ggcgctcgtt cttcaacggc gaggtcttcc
     1201 gcgactcgtt cccggacatc acggcgaagt tcctcaagaa cgtgggcgcg ttcctcatcg
     1261 cagaggtctt catcctcgtg ctggccctgt tcatcgccgt gctgcggagc ctgcccgggc
     1321 ccgtcttctt cccgatccgg accctggccc tcgggtacac ggacctgttc cggggcatcc
     1381 ccacgatcct cgtgatctac atcctcgggt tcggcgcacc ggccctgcag cttcaggggg
     1441 tccccaagga tcccttcttc tgggggatcg tggcgctcgt gctcgtgtac tcggcctatg
     1501 tggcggaggt gtatcgggcg gggatcgagt cggtgcaccc gagccaggtc gcggcggcgc
     1561 ggtcgctcgg gctgacccgc tggcagggcc tgcgtttcgt cgtccttccc caggcggtcc
     1621 gccgcgtcgt ccccccgctg ctgaacgact tcatcgggct gcagaaggac acggcgctgc
     1681 tggcgctgat cggcgtgctc gagacgttcc gtcagtcgca ggtcgacgtc gccggctcct
     1741 tcaactacac gccgtacctg gcgaccgcga tgctcttcgt cctgatcacg atcccgctcg
     1801 ccaggttcac cgactggctg atcgctcggg agcggcggcg acaccaggcg gggagcgcgc
     1861 tgtgacccgg gccctgaccc tggaaggcgt ccacaagtcg ttcggcaagg tcgaggtgct
     1921 gcggggcgtg gacctggcgg tggaccagca cgaggtgatc gcgctgatcg gcgcctcggg
     1981 ctcggggaag tcgacgctgc tgcgctgtgt gaatcttctc gagcccgtcg atgcgggacg
     2041 catcctcctg aacgaggacg agatcacgct ccgcggcgtg aacgagaacg aggtccggaa
     2101 gcggatcggg atcgtgttcc aggccttcaa cctcttcccg cacatgacgg tcctgcggaa
     2161 cgtcaccttg ggaccgatcg aggcgctggg gctgtcccgc gaggaggccg aagcgcgcgc
     2221 ggaggaactc ctcgcgcgct tcggcctcct cgacaagcgc gacgagtacc cggaccgcct
     2281 ctcgggcggc cagcagcagc gggcggcgat cgtccgcgcg atggcgatgc agcccgacct
     2341 cctcctcctg gacgaggtga cgagcgcgct cgatcccgag ctgatcgccg aggtgctcga
     2401 cgtgatccgg gaactggcgg aggcggggat gacgatgatc ctggccacgc acgagatggg
     2461 cttcgccaag gacatcgccg gccgggtgag cttcctggac gagggcgtga tcctggagga
     2521 gggtcccccc gacaggatgt tcgccgagcc cgagcacccc cgcacgcgcc ggttcctcca
     2581 gcggatcatc gccgcgggcc ggctctgact atcctgtggc ccgatgagcg aggaccgccg
     2641 cgaacccgag gagcgccgcc gctcggaggt tcgcctgcgg atcgaggagc tgcgcgagca
     2701 gatccgccac caccagtacc gctactacgt cgaggacgac ccagaggtct ccgacgcaga
     2761 gtacgacgag ctcgtccggg agctggagaa gctcgaggtc gagttccccg agttcgtcac
     2821 gcccgacagc cccacccagc gcgtgggggg ccaggtgtcc gagctcttcg cgccggtgga
     2881 gcaccgcacc ccgatgctct ccctcgacaa cgcgttcacg tgggaggagc tgagcgagtg
     2941 gggccgccgc gtagagcggg tcgtggggcg ggccggcgac ttcgtgtgcg agctgaagat
     3001 cgacggagtc gctgtggccc tcacgtacga ggacggtcga tacgtgcggg gggccactcg
     3061 aggcgacggc tacacgggcg aggacatcac ggccaacatc cggacggtgg ggggcgtccc
     3121 gatgcgcctc cgcggcgagc attcgggcgt actcgaggtc cgcggcgagg tgtacctccc
     3181 cgtgaaggcc ttcgaggaga tgaaccggga gctgcaggag gccggcggcc gggccttcgc
     3241 caacccgcgc aacgccgcgg ccggctcgct ccggcagaag gaccccggcg tgacggctcg
     3301 caggccgctg cgactgtggg tgcacgggct gggctgggcg gacggacggc ggttctcgcg
     3361 gcactccgag gccctcgatc acctgcggga ggcggggctg cccgtcaacc cggacaacgc
     3421 cctgctcgac acgctggagg acgtgttcgc gttctgcgag aggtggcagg agcggcgaca
     3481 cgacgtcgac tacgagatcg acggcgtggt ggtgaaggtg gaccagatct cgctccagga
     3541 ggagctgggc gccacggcga aggccccgcg ctgggcgatc gcctacaagt tcccgcccga
     3601 ggagcggacg accctgctgc gggggatctt cacgcacacg ggccggacgg ggaaggtgac
     3661 gccgttcgcg cagctcgagc cggtgttcgt gggcggggtc acggtgacca cggcgaccct
     3721 gcacaacgag tccgaggtgg cgaggaagga cgtccgcccc ggcgaccacg tgatcgtgcg
     3781 gcgagcgggc gacgtgatcc ccgaggtcgt cggcccggtg atgcagaagc ggaagcgagg
     3841 cgcgcggcgc tggaagttcc ccaaggagtg tccctcgtgc gggacgcctc tcgtccgcga
     3901 ggagggcgag gccgactggc gctgcccgaa ccgcgagggg tgcccctcgc agaacatcga
     3961 gtggctcttc cacttcgcgg ggcggggggc gatggacatc gagcacctcg gctacaagac
     4021 ggggatggcg ctcatggatc gcgggtgggt gaccgaccca gccgatgtct acttcctgac
     4081 cgaggagcag ctcgcggagc tccccggctt caaggagaag tcgatcggca acctgctggg
     4141 ggcgatcgag gattcgaagg accgtccgat ctggcgcctg ctcgtggggc tcaacgtgcg
     4201 ccacgtgggg acgacggtgg cgcggatgct gacgcgggcc ttcccctcga tcgtggctct
     4261 gtcgaaggcg agcgcggagg acatcgaggc ggtcgagggg atcgggccgg agatcgcccg
     4321 gagcgtccac gagtggttcc ggcgcccgga gtcgaagcgg ctgctgaaga agctgaagaa
     4381 ggccggggtg cggatggagg atcccgagcc agaggggccg gccccggacg ggccgctcgt
     4441 ggggaagcgg gtggtgctca cgggcgggct ggagtcgatg agtcgcgacg aggccacgaa
     4501 ggcgtccgag gccgcgggcg cccgggtgac gtcgagcgtg tcgaagagca ccgacttcgt
     4561 cgtggcgggg accgacccgg gctcgaagta cgacaaggcc gtgtcgctcg gcgtcgagat
     4621 catcgacgag caagagttcc gtaggcgcct cggcggctga ggcgccgggg gacggcccag
     4681 gagggaggcc cttgccgccg gagggcagcc atgcgggcgc ccgcgtccag gtcctcgggg
     4741 agccccccgc ttcgcctcat cgcgatcagg tggtcggcgc aggctacgaa gaggaggccc
     4801 gaggggatcc gcaggagctc agccgcatcg tggagctcgt cgaagccgcg gatgggacgt
     4861 ggaggagtga atcggacgct gagcgtgccg gttcgtccga ggcgtcgggc gatcgtgcgt
     4921 cgcacgagat cgagccgccg ccggttgaga tggtccgagc gcgcacagat ctggacgggc
     4981 ccggcggaga cgtcggagcc gtggaggcgc tcggcgagcg ggccgatcag gacgcagcgg
     5041 acccgccgga acgcgaggta gcgcaggagc tcgtgctggt tgccgttcag tgggcgtgcg
     5101 ggcgtccgga tcgctggctc gggacccggg cgatgctcgg cgacgagatc ggccccacag
     5161 gcgtcgagga gtcggacgag ggtatcggca cgggggatct ggtcgccccg ctcgatgcgt
     5221 gcgatgttgg cctggtgggt gccggcgcgg cgggccagct cggcctgcgt gagaccggcg
     5281 aggatgcggt gccttctgat ggtgtagccg acatccatgg tgtagtcgcc atgatgggct
     5341 gagggtccga cacggtcggc ggtcggaggc ggcggtcgga cgcttgcgcg ccccactggt
     5401 gggagctcgt ggtctgcgcg atcgcgggag ccgacggggc acccctccac gccgacccat
     5461 caggaattca tggcgaagtc gccatgtttg cggcggtcct gccctttgcc gcgctcggcg
     5521 tcgcgggatc atggcgaggc cgccatattc ctcagagggg ccggcgtggc gcttccctca
     5581 cccttcgagc cgatcgcggg cccgatcgac gtcccccagc cgaccgcccc ccagccgacc
     5641 gccccccagc cggccgtccc cgccggttcg ctagactcgg cgccatgccg gtcgagatcg
     5701 acatcgagca cgtggcgcgt ctggcgaggc tcgccctcac ccccgaggag ctggagcggt
     5761 tccgagagca gctcggggtg atcctggagc acgcggagcg ggtcaccgag gtcgcggccg
     5821 aggccgtccc ggcgaccgcg cacccgatcc ctatggccaa cgtgttccgg cccgacgagc
     5881 cgcgggacgg cctcggccgc gacgaggccc tggctaatgc gcccgatcgg gagggcgact
     5941 tcttcaaggt cccgcccatc gtggaagagc cggagtgacg ggggagcccg ggtgaccgag
     6001 ctctgcgacc tgaccggcca cgagctggcg ggaaagctcc gcgcggggga ggtctcggcc
     6061 gtggacgggg tcgagtcgtc cctgtcccgg atcgaggcca ccgagccgag cctccacgcg
     6121 ttcctggccc gctccgccga tctggcccgt gagcaggctc gcgcggtcga tgaccggctc
     6181 gccgccgggg acgacctccc tcccgtggcc ggcatccccc tcgcgctcaa ggacgtcctc
     6241 accacgcgag ggatcaccac cacctgcggc tcgcggatcc tcgagaccta cgtgcccccg
     6301 tatgactcga cggtctgggc acggctgagg gaggcgggct cggtcctcgt gggcaagacg
     6361 aactgcgacg agttcgcgat gggctcctcg aacgagaact ccgccttcgg gccggtccac
     6421 aacgcgtggg atctggaccg cgtgcccgga gggtcgtcgg gtgggtcggc ggcggcggtg
     6481 gccgcgggcc aggccgtctg ggcgctgggg accgacaccg gcggctccgt gcgccagccc
     6541 gccgcgctgt ccggcgtggt cggcatgaaa cccacctacg gccgcccctc gcggtacggg
     6601 ctgatcgcgt tcgcctcgtc gctcgacacc gtctcgacct tcacccgcga cgtgcgcgat
     6661 gcggccacgc tgctgtcggc gatcgtgggg cacgaccccc tcgacgccac gagcatccag
     6721 cggcccgccg aggactacac ggagggcctc gagcagggcg tcgacggcct ccggatcggc
     6781 gtcctccgcg attggctggg cgagggcgtg gagccgggcg tccgcgaccg cgtggaggag
     6841 gcgatccagc gcctggaggg cctcggggcg aagatcggcg aggccaccct tccccacgcc
     6901 gactacgcgc tcagcgccta ctacctgatc gctcccgccg agtgctcctc gaacctcgcc
     6961 cgctacgacg gggtgcgcta cggccaccgc gccgagggcg ccaacgtcgt cgagatgatg
     7021 atgaggacgc ggggcgaggg attcggggac gaggtcaagc gccgcatcat gctcgggacc
     7081 tacgcgctct ccgcgggcta ctacgaggcc tactacggcc aggcccagaa ggtccggact
     7141 ctggtcctcc gcgacttcga ggccgcgtgg caggagttcg atctcctgct gtctccgacc
     7201 tcgcccacgg tcgccttccc gctcggggcc aaggccgacg acccgctggc gatgtacctc
     7261 tccgacgtct acacgatccc cgccaacctg gcgggggtgc ccgccgtcag cgtcccgtgt
     7321 ggcctcgacg accaggggct gcccgtgggc ctccagctga tgggcagggc tctggacgag
     7381 gccacggtgc tccgcgcggc ctacgcgttc gagcaggatc tcggcttcga cgcgcggcct
     7441 ccgctgctcg aggggatcgc ctcgtgaccg cctacgagac cgtgatcggc ctggagtgcc
     7501 acgtcgagct ctcgacgaag acgaagatgt tctgctcgtg ccggaacgag ttcggggccg
     7561 agcccaacac gctcgtctgc ccgatctgcc tcggccaccc gggcaccctt cccgtcccca
     7621 acgagcgggc cgtcgagcac acgatcacga tcgggctcgc cctgggatcg gagatcgcgc
     7681 ccacctcgct cttccaccgg aagaactact tctatccgga catgccgaag aactaccaga
     7741 tcagccagta cgacctgccg ctgtgcgtgg gcggcgcgct ggaggtcgag gtcgacggcg
     7801 agccgcggcg cgtcgggatc acccgcgtgc acctcgaaga ggacacgggg aagaccaccc
     7861 acgccggcgc caccggacgc atcgccggcg ccgactatgc gctggaggat ttcaaccggg
     7921 cgggcgtccc gctcgtggag gtcgtctcgg agcccgatct ccgctcgccg gccgaggccc
     7981 gcgcctacct cgtggcgctg cgcgggctgc tccgctcgct cggcgtctcc gacgtgaaga
     8041 tggaggaggg ctcgctccga tgcgacgcga acgtctcggt tcggccggag ggtcacgagg
     8101 agctgggcac gaaggtcgag atcaagaaca tgaactcgat ccggagcgtg gagcgggcgc
     8161 tggccttcga ggccgagcgc cagcgggcgg cgctggacgc gggggagacg ctggtccagg
     8221 agacgcgcca ctgggacgag gaccagggtg agacgtcctc gatgcggtcg aaggaggagg
     8281 cgttcgacta ccggtacttc cccgatcccg atctctcgcc gctggagccc gaggccgagc
     8341 gggtggaccg gctgcggtcg gagctgcccg agcttccggc cgcgcgccgg gcgcgcctgg
     8401 agtccgagct gggggtgccg gctgcccagg ccgcccaggt cgtggagcag ggatgggaca
     8461 ggttcctccg ggaggccgtc gcggccggcg ccgacgcgaa gggggcggcg aactggatga
     8521 cgggcgacct cgcggcgctg ctgaacgagg ctggcatcga gctcggccag gcgaaggtgt
     8581 cgccgaagca tctcgcggat ctcgtccggc ttgtggccga cggcacgatc tccaccgcgg
     8641 gtgcgaagca ggtgctggcg gagatgttcg cctcgggggc ggatgccgaa gccgtggtcg
     8701 aggagcgcgg cctggcccag atctccgacg agtccgcgct cggggcgatc gtggacgagg
     8761 tgatcgccga caaccccgat ccggtcgagc agttccgcgg cggcaaggag ggcgtgatcg
     8821 gcttcctcgt cggccaggtg atgcagcgct ccaagggcgc cgcgaacccc caggaggccc
     8881 agcgcctcct ccgcgagcgc ctagcccgat aggcctattg acgcggccca ctca
//
LOCUS       DASUTW010000151         6351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_695669, whole genome shotgun sequence.
ACCESSION   DASUTW010000151 DASUTW010000000
VERSION     DASUTW010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695669"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..159
                     /locus_tag="VF058_04960"
     CDS             1..159
                     /locus_tag="VF058_04960"
                     /inference="COORDINATES: protein motif:HMM:NF019275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX6207692.1"
                     /translation="MIGKGGEMLKRIGTEARRELEALLDEKVYLELRVKVLHDWQRDP
                     KALQRLGY"
     gene            complement(156..665)
                     /locus_tag="VF058_04965"
     CDS             complement(156..665)
                     /locus_tag="VF058_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207693.1"
                     /translation="MKRIAIVLTLVVGTAGCAEVGEDRTPQAGPEISPSPSIGGATLT
                     LVAAPDGPLARLRSGGQEVGVDAFTSCWDSPGVSTCVDGFPTEPEAYVGVRRGGTLTV
                     SGDAETVEVGVARFRPGDVAAELEVVQDLPLEDGSATIDVEPGRYLLQVFATFEEGDG
                     AFALGLRVL"
     gene            complement(704..1153)
                     /locus_tag="VF058_04970"
     CDS             complement(704..1153)
                     /locus_tag="VF058_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX6207694.1"
                     /translation="MMKNIALIAIMTALLATACEAEVEDPEAGPSPAVTVDDETATGD
                     VDVDATPTGCAEVSSAGGAPAPLTMRDSFFEPNCLVVSSTQEMSLVNAGDLPHNFSIR
                     DTDVSEDVDPGEEEESEDLFENLDAGTHEFFCRFHEQEGMVGTIVIE"
     gene            1171..1587
                     /locus_tag="VF058_04975"
     CDS             1171..1587
                     /locus_tag="VF058_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207695.1"
                     /translation="MSRLTKQTKLVALVALFALATGCGGDEGIVGPRDVELESVLADP
                     EGHDGERLRFRAFYVGSFELSVLTAALAESYPPQPAEPTIWVDASPRGGCIVTDVGVT
                     WGEVVAEGVFRYDEGGGFGHLGIYRMQLEGARLACP"
     gene            1654..1956
                     /locus_tag="VF058_04980"
     CDS             1654..1956
                     /locus_tag="VF058_04980"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEX6207696.1"
                     /translation="MSAGHKREIQGPRAVLRFIGRSGKRIGVTIVGFLLLLAGGIMIV
                     TPGPGTVAIIAGLAILATEYTWAEIMLDKVKEKAKQAGSVVRNRSFGRKRDPEPPA"
     gene            complement(1953..2039)
                     /locus_tag="VF058_04985"
     CDS             complement(1953..2039)
                     /locus_tag="VF058_04985"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207697.1"
                     /translation="MWAFHCHVLTHAEGPDGMFGMVTALIVD"
     gene            complement(2012..2710)
                     /locus_tag="VF058_04990"
     CDS             complement(2012..2710)
                     /locus_tag="VF058_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207698.1"
                     /translation="MSSIRSLRRYASTVAVTVFTAVSVAGLPALAHVGGTVGHLWTKH
                     IRPKTDARYFKKAQLRADDGAVNQTGDPVHWTKLRGVPAGFADGVDDAGGGGPATDVQ
                     CAGCVGTSDLGNASVTLGKLGFDPATQEELEAHAGSDDHDGRYFTETETATSDGTPPN
                     AGSNRVRWDNLAGVPAGFADGIDDTGGGGSGWALTGNAGTNPATDFLGTTDAQAMSIR
                     VPPSPACGRSTATC"
     gene            2804..3553
                     /gene="recO"
                     /locus_tag="VF058_04995"
     CDS             2804..3553
                     /gene="recO"
                     /locus_tag="VF058_04995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX6207699.1"
                     /translation="MPLYKAQGIVLGSFKLGEADKILTILTQEEGKVRAVAKGIRRTK
                     SRFGARLEPFTHVDLLLYRGRNLDTVTQAEILAPFRRIREDYALFAAGEAMLEATDKV
                     AEEHERNVRLFLLLRKGLQALDAEPADPAAVAEAFILKLLGLSGFGPILGACAACGGG
                     PVTRFSNEQGGSVCESCRDRDARKASPEALAWLRELAEGDLDEAGDATPALRVRGEAR
                     ALLYGFTEHHLERRMRSLPLLARVEPRAALP"
     gene            3550..4269
                     /gene="uppS"
                     /locus_tag="VF058_05000"
     CDS             3550..4269
                     /gene="uppS"
                     /locus_tag="VF058_05000"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004600329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX6207700.1"
                     /translation="MRDLDPDRLPAHVAIVMDGNGRWARERGLPRTAGHEQGERALYD
                     VVDGALEIGLRHLTVFAFSTENWRRPPSEIRFLMGFNRRIIHSRRDELHERGVRIRFL
                     GRRGRPLPRSVLREMEEAERLTARNDRMTFTVALNYGGRIEVVDAVRRLLEDHDAGRL
                     RGRITERSIAARLYDPELPDPDLMIRTSGEQRLSNFLLWQSAYTELWFTPVLWPDFTR
                     EHLFEAIREYQRRERRFGGVT"
     gene            4313..4771
                     /locus_tag="VF058_05005"
     CDS             4313..4771
                     /locus_tag="VF058_05005"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX6207701.1"
                     /translation="MRYFAYGANMHPREMAKTSPIATVVGPGLVEGFRLTFGVYSDRW
                     KGGAANIMPAEGGRCWGVVWDVPEKDVVKLDTFAGHPTFYRHDDVVVHVGGSLETCTT
                     YRIAHQHGYVRPDQAYLQVLRAAIREQGLPVEALDLLERAASLPYPRIDS"
     gene            4833..5606
                     /locus_tag="VF058_05010"
     CDS             4833..5606
                     /locus_tag="VF058_05010"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX6207702.1"
                     /translation="MMNPPQAALDRRGPLPLYFQLREALLRHVRDRGLQPGDRLPTEA
                     ELERRYGVSRSTIRQAMNELAAEGLVHRVQGKGTFLGTPKIQHVPVLTSYSELLRSQG
                     YRPSHRLLVSATRPALPEVAEALGIEEGETCRFLRRLFLADDDPVGLAETWLPQDVLG
                     EHDALLERGAIEDGSLYELLQAEPIALRLHRAVETINPGVAEDGEAGLLGCEEGSPLL
                     VIHRITYTPEDRPIEWTRLLFAGGRYEYRVEMHRPGGTS"
     gene            5603..6196
                     /gene="rpiB"
                     /locus_tag="VF058_05015"
     CDS             5603..6196
                     /gene="rpiB"
                     /locus_tag="VF058_05015"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX6207703.1"
                     /translation="MSERRSPEEVRAVVATVVDAVAGEIHEEPRAAEPAAPSPDGIPS
                     LADVAVGADHGGFQLKQRIAAELRDHGFSVQDCGTEGSDSVDYPDFAYEVARLVAKGA
                     CRWGIVVDGAGIGSAITANKVPGVRAATCWDVSSARNSREHNHANVLALGAGLVGEAL
                     ALQIVQAWLSTGWGGGRHARRVSKIDKIERTYTRATA"
BASE COUNT          904 a         2272 c         2259 g          916 t
ORIGIN      
        1 gtgatcggga agggcggcga gatgctgaag cggatcggca cggaggcccg ccgggagctc
       61 gaggcgctcc tcgacgagaa ggtctacctc gagctccgcg tcaaggtgct gcacgactgg
      121 cagcgggacc ccaaggcgct ccagcggctg ggctactaga gcacccgaag accgagggcg
      181 aacgcgccgt cgccttcctc gaaggtggcg aacacctgga gcaggtacct ccccggctcc
      241 acatcgatcg tggcggagcc gtcttcgagc gggaggtcct gcaccacctc gagctcggcg
      301 gcgacgtcgc cgggccggaa ccgagccacc cccacctcga cggtctctgc gtctcccgac
      361 acggtcagcg tcccgccgcg gcgcaccccc acgtacgcct ccggctcggt cgggaagcca
      421 tcgacgcacg tggagacccc cggcgagtcc cagcacgagg tgaacgcgtc gacccccacc
      481 tcctggcccc cgctcctcag ccgggcgagg ggtccgtccg gggcggccac gagcgtcagc
      541 gtggccccgc cgatggatgg agagggtgag atctcgggac cggcctgggg tgtccggtcc
      601 tcgccgacct ccgcgcatcc ggctgtgccc acgacgagcg tcaggacgat cgcgatcctt
      661 ttcatgtcgg gtcagacccg gggacgggcc gggaggttcc cgcctactcg atcacgatcg
      721 tcccgaccat gccctcctgc tcgtggaacc ggcagaagaa ctcgtgggtg cccgcgtcga
      781 ggttctcgaa gaggtcctcg gactcctcct cctcgccggg atccacgtcc tcgctgacgt
      841 cggtgtcccg gatcgagaag ttgtgcggca ggtcgccggc gttcacgagc gacatctcct
      901 gggtcgagga cacgaccagg cagttcggct cgaagaacga gtctctcatc gtgagcgggg
      961 cgggagcgcc gcccgcgctc gagacctcgg cgcacccggt gggcgtggca tccacgtcca
     1021 cgtcgcccgt ggcggtctcg tcgtcgaccg tcacggccgg gctcgggccc gcctccgggt
     1081 cctcgacctc cgcctcgcac gccgtcgcca ggagggccgt catgatggca atcagagcga
     1141 tgttcttcat catggaagcg ggcatacccg atgagcaggc tcacgaaaca gacgaagctg
     1201 gtcgccctgg tcgcgctgtt cgcgctggcg accggctgcg ggggcgacga gggtatcgtc
     1261 gggccgcgtg acgtcgagct cgagagcgtc ctggccgacc ccgagggtca cgacggcgag
     1321 cgtctgcggt tccgggcgtt ctacgtgggg tcgttcgagc tgagcgtcct caccgcggcg
     1381 ctggccgagt cgtacccgcc ccagcccgcc gagcccacga tctgggtgga cgcgagcccc
     1441 cggggcggat gcatcgtcac cgacgtcggg gtgacgtggg gcgaggtcgt cgcggagggg
     1501 gtcttccggt acgacgaggg gggcggcttc ggccacctcg ggatctacag gatgcagctc
     1561 gagggcgccc ggctggcctg tccctgacgc tccgcggatg ttcgatatca tcgcgcgcat
     1621 ggatcagcgc gcctcccgac ccccggacgc ctgatgtcgg ccgggcacaa gcgggagatc
     1681 cagggcccca gggcggtcct ccgcttcatc ggccggagcg ggaagcgcat cggggtcacg
     1741 atcgtcgggt tcctgctgct gctggccggc ggcatcatga ttgtgacgcc cgggcccggc
     1801 accgtggcca tcatcgcggg tctggcgatc ctcgccaccg agtacacctg ggccgagatc
     1861 atgctcgaca aggtgaagga gaaggcgaag caggccggca gcgtggtgcg caaccgctcc
     1921 ttcggaagga agcgggaccc ggagccgccc gcctagtcca cgatcagcgc cgtcaccatc
     1981 ccgaacatcc cgtctggtcc ctccgcgtgc gtcagcacgt ggcagtggaa cgcccacacg
     2041 ccgggctcgg tggcacgcgg atcgacatgg cctgagcatc cgtcgtgccc aggaagtccg
     2101 tggcggggtt cgtgccggcg ttccccgtga gcgcccagcc ggacccgccc ccacccgtgt
     2161 cgtcaatccc gtcggcgaac cctgccggga cgcccgcgag gttgtcccag cgcacgcggt
     2221 tcgatcccgc attgggcggc gtcccgtccg acgtcgccgt ctccgtctcg gtgaagtagc
     2281 gcccgtcgtg gtcgtccgat ccggcgtggg cctcgagctc ctcctgcgtg gccgggtcga
     2341 agccgagctt cccgagcgtg accgaggcgt tccccaggtc ggaggtcccc acgcacccgg
     2401 cgcactgcac gtcggtggcc ggcccgccgc cgcccgcgtc gtccactccg tcggcgaatc
     2461 ccgcgggaac gcccctgagc ttcgtccagt ggacggggtc gccggtctgg ttcacggcgc
     2521 cgtcatccgc tcggagctgc gctttcttga agtacctagc gtcggtcttc gggcggatgt
     2581 gtttggtcca gagatgaccg acggtgccgc cgacgtgagc cagcgccgga aggccggcca
     2641 cggatacggc ggtgaagacc gtcaccgcca cggtcgaggc gtagcggcgg agcgaacgga
     2701 tggagctcat cctgcacctc ctggaggtcg cctcgcgcga gtatgccctc aggcggtgaa
     2761 ggcttgccta gggcggcgga tccccgctga tacgatgcgg tcgatgccgc tctacaaggc
     2821 ccaggggatc gtgctcggct ccttcaagct gggggaggcc gacaagatcc tcacgatcct
     2881 cacccaggag gagggcaagg tccgggccgt ggccaagggg atccggcgga cgaagagccg
     2941 cttcggagcc cgcctggagc ccttcaccca cgtggacctg ctgctctacc ggggccgcaa
     3001 cctcgacacg gtcacccagg ccgagatcct ggcccctttc cggaggatcc gcgaggacta
     3061 cgcgctgttc gcggcggggg aggcgatgct cgaggcgacc gacaaggtag ccgaggagca
     3121 cgagcggaac gtccggctct tcctcctcct ccggaagggc ctccaggcgc tcgacgctga
     3181 gccggcggac ccggcggcgg tggccgaggc gttcatcctg aagctgctgg ggctgtccgg
     3241 cttcggcccg atcctcggcg cctgcgccgc gtgcggcggc ggtcccgtga cccgcttctc
     3301 gaacgaacag gggggctcag tgtgcgagtc gtgccgcgat cgtgacgccc ggaaggcctc
     3361 ccccgaggcg ctggcctggc tccgcgaact cgccgaggga gacctggacg aggccggcga
     3421 tgcgaccccg gccctgcggg tccgaggaga ggctcgggcc ctcctctacg gattcacgga
     3481 gcaccacctc gagcgccgca tgcgcagcct ccccctgctg gcgcgggtcg agccccgcgc
     3541 cgccctcccg tgagggacct cgatcccgac cgcctcccgg cccacgtcgc catcgtcatg
     3601 gacggcaacg gccgctgggc tcgggagcgc ggtctccccc ggacggccgg ccacgagcag
     3661 ggcgagcggg ccctctacga cgtggtggac ggagctttgg agatcgggct ccgccatctc
     3721 accgtcttcg ccttctccac cgagaactgg cggcggccgc cctccgagat ccgcttcctg
     3781 atgggcttca accggcggat catccacagc aggcgcgatg agctccacga gcggggggtg
     3841 cggatccggt tcctcgggcg gcgggggagg cccctgcccc gctcggtcct tcgggagatg
     3901 gaggaggcgg agcgcctgac cgcccgcaac gaccgcatga cgttcacggt cgcgctcaac
     3961 tacgggggcc ggatcgaggt ggtggacgcc gtccgccggc tcctcgagga tcacgacgcc
     4021 ggccgcctgc gcggccgcat caccgagcgc tcgatcgcgg cgcgcctcta cgacccggag
     4081 ctccccgacc ccgacctcat gatccggacc tcgggggagc agcggctctc gaacttcctc
     4141 ctctggcaga gcgcctacac cgagctctgg ttcacgcccg tgctgtggcc ggacttcacc
     4201 cgagagcatc tcttcgaggc gatccgcgag taccagaggc gcgagcgccg cttcgggggc
     4261 gtcacctgag gccggattcg cggcgttcac cgggcggggc tagcatcgtc ccgtgcggta
     4321 cttcgcctac ggcgcgaaca tgcacccccg ggagatggcg aagacctctc ccatcgcgac
     4381 ggtggtgggc cccgggctcg tggagggctt ccggctgacc ttcggcgtct actcggaccg
     4441 ctggaagggc ggggccgcca acatcatgcc cgctgagggg ggccggtgct ggggcgtggt
     4501 gtgggacgtg cccgagaagg acgtggtcaa gctcgacacg ttcgccggcc atccgacctt
     4561 ctaccgccac gacgacgtgg tcgtccacgt cgggggatcc ctcgagacct gcacgaccta
     4621 ccggatcgcc caccagcacg ggtacgtgag gcccgaccag gcctacctcc aggtccttcg
     4681 ggcggcgatc cgcgagcagg gcctcccggt ggaggccctc gacctgctgg aacgggccgc
     4741 ctcgctcccg tacccgcgca tcgatagctg atcctcctcc gcccccaagt gcttgacagc
     4801 ggcgcgaccg tagagcatga tgacatatca tcatgatgaa tcctccccag gccgctctcg
     4861 atcggcgggg gccgctcccc ctctacttcc agctgcgcga ggccctcctg cgccacgtcc
     4921 gcgaccgtgg cctccagccc ggcgaccgcc tccccaccga ggccgagctc gagcgccgct
     4981 acggcgtgtc gcgctccacg atccgtcagg ccatgaacga gctggccgcc gagggcctcg
     5041 tccaccgggt ccaggggaag ggcacgttcc tgggcacccc gaagatccag cacgttccgg
     5101 tgctgacctc gtactccgag ctgctgcgga gccaggggta ccgcccctcc caccgcctgc
     5161 tggtctcggc gacccgcccg gcgctccccg aggtggccga ggccctgggg atcgaggagg
     5221 gcgagacctg ccggttcctg cggcggctct tcctggccga cgacgacccg gtcggcttgg
     5281 ccgagacctg gctcccgcag gacgtcctcg gggagcacga cgccctgctg gagcggggcg
     5341 cgatcgagga cggctcgctc tacgagctgc tgcaggccga gccgatcgcc ctgcgcctgc
     5401 accgcgccgt cgagacgatc aacccgggcg tggccgagga cggcgaggcc gggctcctcg
     5461 ggtgcgagga ggggagcccg ctcctcgtga tccaccgcat cacgtacacc cccgaggatc
     5521 gtccgatcga gtggacgcgc ctgctgttcg ccggcggcag gtacgagtac cgggtcgaga
     5581 tgcaccgtcc gggggggacg tcgtgagcga gcggcggagt cccgaggagg tccgggcggt
     5641 cgtggccacg gtggtggacg cggtggcggg cgagatccac gaggagccgc gcgccgccga
     5701 gccggcggct ccgtcgccgg acggaatccc gtccttggcc gacgtggccg tcggcgccga
     5761 ccacgggggc ttccagctga agcagcggat cgcggccgag ctgagggacc acgggttcag
     5821 cgtccaggac tgcgggacgg agggatccga ctcggtggac taccccgact tcgcgtacga
     5881 ggtggcgcgg ctggtggcca agggcgcgtg ccggtggggg atcgtcgtgg acggcgcggg
     5941 gatcggctcc gcgatcaccg ccaacaaggt ccccggcgtg cgcgccgcga cctgttggga
     6001 cgtctcgtcc gcgcggaaca gccgcgagca caaccacgcg aacgtcttgg cgctcggcgc
     6061 cgggctcgtg ggcgaggcgc tggccctgca gatcgtgcag gcgtggctct ccacgggctg
     6121 gggcgggggc cggcacgcac ggcgcgtgtc gaagatcgac aagatcgagc gaacctacac
     6181 gagggcgacg gcatgagcgc gcgcgaggat ctgatcgagg ccgtcaccag cgaggtgctg
     6241 gccgcgctgg ccccggccga gcgctgtccc gactgccgcg gctcctgcgc ggcccacgtc
     6301 ccggacaagg tccgggccat cgtggacaac ggcgcccacc gcatcagcta c
//
LOCUS       DASUTW010000152         8721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1731576, whole genome shotgun sequence.
ACCESSION   DASUTW010000152 DASUTW010000000
VERSION     DASUTW010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8721
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731576"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..1019
                     /locus_tag="VF058_05020"
     CDS             240..1019
                     /locus_tag="VF058_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6207704.1"
                     /translation="MGDLVRLEVEGGVGTIRLDRPPMNAIDEDLTRDLLAVTREAARR
                     DDVGACVLFGGEKVFAAGADVKAMAEMGVAEVRPYIQSLQEVYNAVEDLPKVTIAAIT
                     GFALGGGCELAMCADFRFAAENARIGQPEIQLGIIPGAGGTQRLPRLVGPARAKDLIY
                     TGRHVRGEEAKAIGLVDEVHPPREVYEAAVEAARRFANGPRVALAAAKNAINWGGRVD
                     LRTGVAFEREVFIDLFTTEDQKAGMRSFVEEGPGKATFSGR"
     gene            1140..1343
                     /locus_tag="VF058_05025"
     CDS             1140..1343
                     /locus_tag="VF058_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207705.1"
                     /translation="METTSGLRGSRPRELPRPNRTYGEDRVCAQDGCDTRLSRYNKSK
                     HCFTHTPLTFPLVRGERKRKSAA"
     gene            complement(1347..1580)
                     /locus_tag="VF058_05030"
     CDS             complement(1347..1580)
                     /locus_tag="VF058_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207706.1"
                     /translation="MADRTRTVHLGQFTWEAANDIAGALEEAGIVWWYKQPGFFSQIW
                     EHGIRLFVDRERLAEAREIARRVIEELESPTES"
     gene            complement(1591..2013)
                     /locus_tag="VF058_05035"
     CDS             complement(1591..2013)
                     /locus_tag="VF058_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207707.1"
                     /translation="MIRRLLWVMVLLAAGAAAGYVWRDARPQAAAPRDAPPVIQQNDP
                     DPGGPPVIWLSGTLREVTDERLTLQEGVGPTLDLTRFAGGATRFHRLGPGGWVRVAPP
                     AADATAGQDACIEALLDQGELLAIRVFLGATCSPAVPG"
     gene            complement(2028..3350)
                     /locus_tag="VF058_05040"
     CDS             complement(2028..3350)
                     /locus_tag="VF058_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207708.1"
                     /translation="MRRFRLEAVLVVLLVAALALTFVLRGAPVGTGSGDDRETADPLG
                     GATPCLQALFGEPEPGPPAAEADVLSVAPVVEELRELTFERQPEPVYLDPQALSERVT
                     EMVEEDDEFLAIQARLLTELGALPEEIDLDGEIRDLLGEQVAGFYDTDTGELVVVDDA
                     REGLTTIELLTLVHELQHALADQRLRIPDLEALNDEDQDAATAAQALVEGDAQLTTEI
                     YAVRSLSTTDQLAAASQQVPGLGDTPHFLARSLLFPYLEGARFACHLYREGGWEAVDA
                     AYDDLPGSSAEILFPDRYGSGREPVDPPDPRGLPAPWEEQATFTFGAADLLFLFEAPG
                     NDPTNGLTDARDRVRAWSGGEAHVWAWGGRTAVGLVLSASDGEAMCESVATWWRRAAG
                     DAAEATEEVPGAALATDGRSRDGVLYCEDGSVRLGVGPDLAVARAAVR"
     gene            3448..4236
                     /locus_tag="VF058_05045"
     CDS             3448..4236
                     /locus_tag="VF058_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX6207709.1"
                     /translation="MLNIVVGVKRVPDTAAEKKLDEQDFTLDRESVEAIMNPVDEYAI
                     EEAIKLRDAAGEGEVTVLTMGPEPADKEAVRKALALGADKGVLVTDDRLHGSDAMATA
                     YVLALALAQQDFDLMLLGSESTDARTSLVPQALGQLMGMTALTYVKQIEVSDGKVTVH
                     REEEGGHAVVEAELPAIVSVVKGINEPRYATLKGIMAAKQKEVEVLAGDELGIDPEQV
                     GLEGSKTKVLEASPRPPKEKGVVVKDEGDGHEKLADFLQENKFI"
     gene            4236..5216
                     /locus_tag="VF058_05050"
     CDS             4236..5216
                     /locus_tag="VF058_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX6207710.1"
                     /translation="MAEIWTVVDQSGGEPSKVSLQMIAAGRKLAEEMGHDLAGVFLGT
                     GWDTAREKLAKHGLPRAYVAESQDFEDYLVQPAIDAIVKLAQEHQPAAILFASVPLGK
                     DLASGVAAKLESGVLADVTDIEVEGDRLSCRMDVFGGATITKCQVVGDPQVICVKPNA
                     FVAEEASADLAEERIEAEITDDAKAARIVERVQEEAGGRPSVEEAAIIVSGGRGLGGP
                     EHFSLVEELADVLGAGVGASRAAVDAGWYPHQNQVGQTGKTVSPQLYIAVGISGAIQH
                     RAGMQTSKTIVAINKDAEAPIFNLSDFGVVGDLFEVLPKLTEELKKRKGG"
     gene            5221..6669
                     /locus_tag="VF058_05055"
     CDS             5221..6669
                     /locus_tag="VF058_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207711.1"
                     /translation="MARPWDRLSPRDQARLQSHLLRELLTRYVYPFSPFYRRLLDQRG
                     IKPGDVKRLRDIERLPMVSRWDFAATEEDGWRPFRAMLRPDERSLKRWAHRRTLRRIA
                     TEKLLRGDLAAERVLAEEFKPIHLHLPERAGPVMGYTMRDLSGLSQAGARSLAVMGAD
                     RSDVLVSTLPSGPTLPFWHVHYAAQGAGVAALHLGAGESVRPARAAHWMHRAGATVLV
                     SQPGYAEGLLRGAPPETFERLRVLALWSQGGMQGARERFTSRLRAGGAPDAYVVSLLG
                     IPEARVAWAECPPSADRPGVSHGFHTYPDLEYLEVVDPSTGKLQDEERGGEIVYTSLD
                     WRGSALLRYRTGVVARRGITTKPCPGCGRTVPRILPEISYTDWLVRTTGSQGEVRLDL
                     AEVLPVLWEARGVPLWQVEIARGAGPGRADLVTAYLGGAGERDAEQVERALGPFGVRS
                     RPVPLQDLGRRMGMGTERPEERVVVRSSAPPP"
     gene            complement(6666..7307)
                     /locus_tag="VF058_05060"
     CDS             complement(6666..7307)
                     /locus_tag="VF058_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018583088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX6207712.1"
                     /translation="MLIDLSHPIEEGRGAFPGLPPPRIGPILTHEASRDRYQGKAEFF
                     LGHAEIATNTGTYLDSPFHRFRDREDIGGIPLEKVASLPGLVVDASPDPGPVEIPEGD
                     LEGRAVLIRTGRDERWPSDEYWASGPHLGEQAVTALLRGGAALVGVDFGNVDDVEDPS
                     RPAHTRLLEAGVLIVENLRGLGPLPREGFRFTAAPPAVVGAATFPVRAFAEVP"
     gene            7387..7602
                     /locus_tag="VF058_05065"
     CDS             7387..7602
                     /locus_tag="VF058_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207713.1"
                     /translation="MIDPSKGLVAEGVPERKAWEVRRRGTLAGIVWREGRASFRAAYE
                     RDGRMTQIPRTFPSIEEAARALDRAVR"
     gene            7641..8705
                     /gene="mnmA"
                     /locus_tag="VF058_05070"
     CDS             7641..8705
                     /gene="mnmA"
                     /locus_tag="VF058_05070"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX6207714.1"
                     /translation="MASVLVAMSGGVDSSVAACLLHEAGHQVLGSHMKLVHTDGVEHG
                     CCGPQAEADARAVAEHAGFDFELADLSEVFEERVIADYVGEHERGRTPNPCVRCNERI
                     KFGAFLDRARDLGFDFVATGHYARRARGADGRWRLLRAVDRAKDQSYMLHTLGQEELE
                     RSLFPIGEQTKDETRAHARRLGLPVAGKPDSQELCFVPRADGGAFLEERAPHLVREGE
                     MVDPEGRVLGTHEGSHRFTIGQRRGLGVSTGERAYVLDVDAGANRVVVGPGELLSRRG
                     LIAERVSWVADAPDGPIEAEAQIRYNAPAVPSVVEPLPGDDVRVTFRAPQRAIAPGQS
                     VVFYAAHQLLGGGTISRSLR"
BASE COUNT         1237 a         3058 c         3265 g         1161 t
ORIGIN      
        1 cggaacgggt ctcccgcgac ccatcggctg gagtcccagc tcgggccgac agggtccgcg
       61 tccggtggca cggcgggctc cccgacgggt ccccccaccg gggccgccgc cccacaggcg
      121 ctcagggcga tcgccagcag cgccgccgcg gcgcgtgacc tccccatcac gcgtccacgg
      181 tacgacacgg cgtcaactcc gagcgtacga tgtggccgat ccccgacaga ggaggagcga
      241 tgggagacct ggtgcgcctc gaggtcgagg gcggcgtggg cacgatccgg ctggaccgcc
      301 cgccgatgaa cgcgatcgac gaggacctca cccgagacct gctcgcggtc acgcgggagg
      361 cggcccggcg cgacgacgtg ggggcgtgcg tcctcttcgg gggcgagaag gtcttcgcgg
      421 ccggggccga cgtgaaggcg atggccgaga tgggcgtggc cgaggttcgt ccgtacatcc
      481 agtcgctcca ggaggtctac aacgccgtgg aggacctccc caaggtgacg atcgccgcga
      541 tcaccggctt cgccctcgga ggcggctgcg agctggcgat gtgcgcggac ttccgcttcg
      601 ccgccgagaa cgcgcggatc ggccagcccg agatccagct cgggatcatc cccggggccg
      661 gggggaccca gcgcctcccg cggctggtgg ggcccgcccg agccaaggac ctgatctaca
      721 ccgggcggca cgtccgaggc gaggaggcga aggcgatcgg gctcgtggac gaggtgcatc
      781 ccccgagaga ggtctacgaa gccgcggtgg aggccgcccg ccggttcgcg aacgggccgc
      841 gcgtggccct cgccgccgcg aagaacgcga tcaactgggg gggccgcgtg gacctgcgca
      901 ccggcgtcgc cttcgagcgc gaggtcttca tcgatctctt cacgaccgag gaccagaagg
      961 cgggcatgcg ctcgttcgtc gaggagggcc cgggcaaggc cacgttctcg ggccgctagc
     1021 ctggggttgc gctccgccga gccgggtaaa ggtcccctcg acatcggtcg acccatgggg
     1081 aatacgcatg gggttcccct gcgtttcaga ggggacgtcg ccggcgagga gtgagcgaga
     1141 tggagaccac ctcagggctc cgcggttccc gcccccggga gctgccgcgc ccgaaccgaa
     1201 cgtacggcga ggatcgggtg tgcgcccaag acgggtgcga cacgaggctc tcccgctaca
     1261 acaagtcgaa gcactgcttc acgcacaccc cgctcacgtt cccgctggtg cgcggggagc
     1321 ggaagcggaa gtccgccgcc tgatcttcag gactcggtcg ggctctccag ctcctcgatc
     1381 acccgtcggg cgatctccct cgcctccgcc aggcgctccc gatccacgaa gagccggatc
     1441 ccgtgctccc agatctggct gaagaagccg ggctgcttgt accaccacac gatgccggcc
     1501 tcctccaggg cgccggcgat gtcgttcgcc gcctcccacg tgaactgacc gaggtgcacg
     1561 gtgcgcgtgc ggtccgccat ggcaccgatg ctacccgggg acggctgggg agcaggtggc
     1621 cccgaggaag acacggatcg ccagcagctc tccctggtcg aggagagcct cgatgcaggc
     1681 gtcctggccc gcggtggcat ccgccgcggg tggcgccacg cggacccatc cacccgggcc
     1741 gagccggtgg aaccgcgtgg cgccgcccgc gaagcgggtc aggtcgaggg tagggcccac
     1801 gccctcctgc agggtgaggc gctcgtcggt cacctcgcgg agggtacccg agagccagat
     1861 caccgggggc ccgcccgggt cgggatcgtt ctgctggatc acgggagggg cgtctcgcgg
     1921 agccgccgcc tggggccgcg cgtcgcgcca gacgtagccg gccgcggcac ccgccgcgag
     1981 caggaccatc acccagagga gcctccggat cacgggccca tcatgcgtca gcggacggcc
     2041 gctcgcgcga cggcgaggtc ggggccgacg ccgagccgga cgctcccatc ctcgcagtac
     2101 agcacgccgt ctcgcgagcg gccgtccgtg gccagcgccg cgcccggcac ctcctccgtg
     2161 gcctcggccg cgtccccggc ggcccgccgc caccaggtcg ccacggactc gcacatcgcc
     2221 tcgccgtcgc tcgccgagag cacgaggccg acggcggtgc ggccgcccca cgcccagacg
     2281 tgggcctccc cgccgctcca ggcccgcacg cgatcgcggg cgtcggtgag cccgttcgtc
     2341 ggatcgttcc ctggggcctc gaagaggaag aggaggtcgg ccgcgccgaa ggtgaacgtg
     2401 gcctgctcct cccagggcgc ggggaggccc cgcgggtcgg gcgggtccac cggctcccgg
     2461 cccgacccgt accgatcggg gaagaggatc tcggcggagc tcccggggag gtcgtcgtag
     2521 gcggcgtcca ccgcctccca cccgccctcc cgatacaggt ggcaggcgaa gcgcgcgccc
     2581 tccaggtagg ggaacaggag actccgggcc aggaagtgag gggtgtcgcc cagcccgggg
     2641 acttgctggg acgctgccgc cagctgatcc gtcgtgctga gcgacctcac cgcgtagatc
     2701 tcggtggtga gctgggcgtc gccctcgacg agcgcctggg cggcggtggc cgcgtcctgg
     2761 tcctcgtcgt tgagcgcctc gaggtcgggg atgcgcagac gctggtcggc cagcgcgtgc
     2821 tggagctcgt gcacgagcgt gagcagctcg atcgtggtga ggccctcccg ggcgtcgtcg
     2881 accacgacga gctcgcccgt atcggtgtcg tagaagcccg ccacctgctc cccgaggagg
     2941 tcccggatct cgccgtcgag gtcgatctcc tcggggagcg cgccgagctc cgtgaggagg
     3001 cgcgcctgga tcgccaggaa ctcgtcgtcc tcctccacca tctcggtgac gcgctcgctc
     3061 agagcctggg ggtccaggta gacgggctcg ggctgccgct cgaacgtcag ctcccggagc
     3121 tcctccacga ccggtgcgac ggagaggacg tcggcctccg ctgcgggcgg gccgggctcg
     3181 ggctctccga acagcgcctg gaggcacggg gtggcccctc ccagcgggtc ggctgtctcc
     3241 cggtcgtcgc ccgagccggt gcccaccggc gctcctcgca gaacgaacgt gagcgcgagc
     3301 gcggccacca gcaggaccac caggaccgcc tcgagccgga agcgccgcat cccgtcactg
     3361 tagccccggg ggggagggtc gacgagccta cccgtgggta ggtagcccgg gatgcgctag
     3421 gatacgcggc ggacatccgg agggaacctg ttgaacatcg tcgtaggcgt gaagcgcgtg
     3481 cccgacacgg cagccgagaa gaagctcgac gagcaggact tcactctcga ccgcgagtcg
     3541 gtcgaggcca tcatgaaccc cgtcgacgag tacgcgatcg aagaggcgat caagctccgc
     3601 gacgccgccg gcgaggggga ggtcaccgtc ctcacgatgg ggcccgagcc cgccgacaag
     3661 gaagctgtgc gcaaggcgct ggccctcggc gccgacaagg gtgtcctcgt caccgacgac
     3721 cgcctccacg gctccgacgc gatggccacc gcctacgtgc tggccctcgc gctggcccag
     3781 caggacttcg acctgatgct gctgggctcg gagtccaccg acgcccggac ctcgctggtg
     3841 ccgcaggccc tgggccagct gatgggcatg accgcgctca cctacgtgaa gcagatcgag
     3901 gtctcggacg gcaaggtcac cgtccaccgc gaggaggagg gcgggcacgc cgtcgtcgag
     3961 gccgagctgc cggccatcgt ctccgtggtg aagggcatca acgagccccg ctacgcgacc
     4021 ctgaagggga tcatggccgc gaagcagaag gaggtcgagg tcctggccgg cgacgagctc
     4081 gggatcgacc ccgagcaggt cgggctcgag ggctcgaaga cgaaggtgct ggaggcctcg
     4141 ccccgcccgc ccaaggagaa gggcgtggtc gtgaaggacg agggggacgg ccacgagaag
     4201 ctggccgact tcctccagga gaacaagttc atctgatggc cgagatctgg accgtcgtcg
     4261 accagagcgg gggcgagccg agcaaggtct cgctccagat gatcgccgcc gggcggaagc
     4321 tggccgagga gatggggcac gacctggccg gggtcttcct cggtacgggc tgggacacgg
     4381 cgagggagaa gctggcgaag cacgggctcc ccagggcgta cgtggccgag tcccaggact
     4441 tcgaggacta cctcgtccag cccgcgatcg acgcgatcgt gaagctggcc caggagcacc
     4501 agcccgcggc gatcctgttc gccagcgtcc cgctggggaa ggacctggcc tcgggcgtcg
     4561 cggccaagct ggagagcggc gtgctggccg acgtcaccga catcgaggtc gagggggacc
     4621 ggctgtcgtg ccgcatggac gtcttcggag gggccaccat cacgaagtgc caggtggtag
     4681 gcgatcccca ggtgatctgc gtcaagccga acgcgttcgt ggccgaggag gcgtcggccg
     4741 acctggccga ggagcggatc gaggccgaga tcacggacga cgccaaggcc gcgaggatcg
     4801 tggagcgggt ccaggaggag gccgggggcc ggcccagcgt cgaggaggcc gccatcatcg
     4861 tctcgggggg tcggggcctg ggcggccccg agcacttctc gctcgtggag gagctggccg
     4921 acgtgctggg cgccggcgtg ggggcctcgc gggcggcggt ggatgccggc tggtacccgc
     4981 atcagaacca ggtggggcag accgggaaga ccgtctcgcc ccagctctac atcgcggtgg
     5041 ggatctcggg cgcgatccag caccgggcgg ggatgcagac ctccaagacg atcgtcgcca
     5101 tcaacaagga cgcggaggcc ccgatcttca acctctccga cttcggtgtc gtgggcgacc
     5161 tcttcgaggt cctcccgaag ctcacggagg agctgaagaa gcgcaagggc gggtgaggcg
     5221 gtggcccgtc cctgggatcg cctctctccc agggaccagg ctcgcctcca gtcgcacctc
     5281 ctgcgcgagc tcctgacgcg ctatgtctac ccgttcagcc cgttctaccg gcggctgctc
     5341 gatcagcggg ggatcaagcc gggcgacgtc aagcggctcc gcgacatcga gcgcctcccg
     5401 atggtctctc gctgggactt cgcggccacc gaggaggacg ggtggcggcc gttccgagcg
     5461 atgctccgtc ccgacgagcg cagcctgaag cggtgggccc accggcggac cctccgccgc
     5521 atcgcgaccg agaagctgct gcgcggcgat ctggccgcgg agcgcgtgct ggccgaggag
     5581 ttcaagccga tccacctcca cctgcccgag cgggcggggc cggtgatggg ctacacgatg
     5641 cgcgacctct cggggctctc gcaggccggc gcgaggagcc tcgcggtcat gggcgcggac
     5701 cggtccgacg tcctcgtctc gaccctcccc tccggaccga cgctcccgtt ctggcacgtc
     5761 cactacgcgg ctcagggcgc cggggtggcg gcgctccacc tgggggccgg ggagtccgtg
     5821 cggccggccc gagcggcgca ctggatgcac cgtgcgggcg ccacggtgct cgtgtcccag
     5881 ccggggtacg cggagggcct gcttcggggg gccccgcccg agacgttcga gcgcctgcgc
     5941 gtgctcgcgc tgtggagcca gggagggatg cagggagcgc gcgagcggtt cacctcgagg
     6001 ctgcgcgcgg gcggggcgcc cgacgcatac gtcgtctccc tgctggggat ccccgaggct
     6061 cgggtggcct gggccgaatg cccgccctcc gcggatcgac cgggcgtctc gcacgggttc
     6121 cacacctatc ccgacctcga gtacctggag gtcgtggacc cctcgacggg gaagctccag
     6181 gacgaggagc gcggtggcga gatcgtctac acctcgctcg actggcgcgg gtcggcgctc
     6241 ctgcggtacc ggaccggtgt cgtggcgagg cgagggatca cgacgaagcc ttgccccggc
     6301 tgcggccgga cggtgcccag gatcctcccg gagatctcct acaccgactg gctggtgcgg
     6361 acgaccgggt cccagggcga ggtgcggctg gacctggccg aggtcctgcc cgtgctgtgg
     6421 gaggcccggg gcgtgccgct gtggcaggtg gagatcgccc ggggggccgg acccggccgc
     6481 gccgatctcg tcaccgccta tctgggcggg gcgggcgagc gcgacgccga gcaggtggag
     6541 cgggcgctgg ggccgttcgg cgtgcggtcc cgcccggtgc cgctccagga cctcggacgg
     6601 cgcatgggca tggggaccga gcgccccgag gagcgggtgg tggtgcggtc gtcggcgccg
     6661 cctccctagg ggacctcggc gaacgcccgc accgggaagg tggcggcgcc gacgacggcc
     6721 ggcggcgcgg cggtgaaccg gaacccctcg cgcggcagcg gccccaggcc ccgcaggttc
     6781 tccacgatga gaacgcccgc ttccagcagg cgcgtgtgcg cgggcctcga cgggtcctcg
     6841 acgtcgtcca cgttcccgaa gtcgaccccc acgagcgcgg cgccgccccg gagcagggcc
     6901 gtgaccgcct gctcgcccag gtgcggcccg ctcgcccagt actcgtcgct cggccaccgc
     6961 tcgtcgcggc cggtgcggat cagcacggcc cgcccctcga ggtctccctc cgggatctcc
     7021 accgggccgg ggtcgggcga ggcgtccacg acgagccccg ggaggctcgc gactttctcc
     7081 agcgggatcc ctccgatgtc ctcgcgatcg cggaaccggt ggaagggcga atcgaggtag
     7141 gttcccgtat tcgtggcgat ctcggcgtgc cccaagaaga actcggcctt cccttgatag
     7201 cggtcgcgcg acgcctcgtg ggtcaggatg ggcccgatgc ggggcggcgg caggccgggg
     7261 aacgcccccc ggccctcttc gatcggatgg ctcaggtcga tcagcatgcg gcgatgctac
     7321 cggttcgtgt ggggacgccc gttcgggtat acctctctca tgccgggccg cgacgaggat
     7381 cgccacgtga tcgacccttc gaagggactc gtggccgagg gcgtgccgga gcggaaggcc
     7441 tgggaggtgc ggcggcgggg tacgctggcc ggcatcgtct ggagggaggg gcgggcctcg
     7501 ttccgtgccg cctacgagcg cgacggccgc atgacccaga tcccccggac gttcccctcg
     7561 atcgaggagg ccgctcgggc gctcgaccgc gcggtccgct gacgaggatg gcggaagccc
     7621 cagtgctacc ctcgacctcg atggccagcg tgctcgtcgc gatgtccgga ggggtcgact
     7681 cctccgtcgc cgcctgcctg ctccacgagg cgggccacca ggtgctgggc tcccacatga
     7741 agctggtcca cacggacggc gtggagcacg ggtgctgcgg gccccaggct gaggcggacg
     7801 ctcgcgcggt ggccgagcac gccgggttcg acttcgagct ggctgacctc tcggaggtct
     7861 tcgaggagcg ggtgatcgcc gactacgtcg gcgagcacga gcgcggccgc acccccaacc
     7921 cctgtgtccg ctgcaacgag cgcatcaagt tcggggcgtt cctcgatcgg gcccgggacc
     7981 tggggttcga cttcgtggcc acgggccact acgcgcggcg cgcgcgcggc gcggatggcc
     8041 ggtggcgcct cctgcgcgcc gtggaccgcg ccaaggacca gtcgtacatg ctgcacacgc
     8101 tggggcagga ggagctcgag cggtccctct tcccgatcgg ggagcagacg aaggatgaga
     8161 cgcgcgctca cgctcggcgg ctcggcctcc ccgtggcggg gaagcccgac tcgcaggagc
     8221 tgtgcttcgt gccgcgtgcc gacggcgggg cgttcctgga ggagcgggcg ccgcacctcg
     8281 tccgcgaggg cgagatggtg gacccggagg ggagggtgct cgggactcat gagggctccc
     8341 accggttcac gatcggccag cggcgaggcc tgggcgtctc gacgggggag cgcgcctacg
     8401 tcctggacgt cgacgcaggc gcgaaccggg tcgtggtcgg gcccggtgag ctgctctccc
     8461 ggcggggcct gatcgccgag cgagtgagct gggtcgccga cgctccggac ggcccgatcg
     8521 aggctgaggc tcagatccgc tacaacgccc cggcggtccc ctcggtcgtc gagccgctcc
     8581 cgggagacga tgtccgcgtc accttccgcg ccccccagcg ggcgatcgcc cccgggcaga
     8641 gcgtcgtgtt ctacgcggcc caccagctgc tcggcggcgg cacgatcagc cgctcgctcc
     8701 gctgaggtcc atcgagccgt t
//
LOCUS       DASUTW010000153         3732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1342940, whole genome shotgun sequence.
ACCESSION   DASUTW010000153 DASUTW010000000
VERSION     DASUTW010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342940"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1140
                     /locus_tag="VF058_05075"
     CDS             160..1140
                     /locus_tag="VF058_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6207715.1"
                     /translation="MIVLVTGGTGFIGSNLVRCLLGAGASVRVLDDLSTGRQENLDGL
                     NGGLDIVVGDVRDGELLASVVRGTEVVYHLAALPSVARSIADPVATHDVNATGTLKVL
                     LSARDAGARRVVYASSSSVYGDTPTLPKHEGMPVAPLSPYAASKLAGEAYCRSFTRSF
                     SLETVALRFFNVFGPRQDPASQYAAVIPRFVNQVLQGESPTVFGDGEQSRDFTYIGNV
                     VQACVLAAEAAAEASGRAMNVGFGRRTTLLELIQILGRIVGREIEPAFAPPRPGDVRD
                     SLADISMAERLIGYRPLMDLRGGLTETVEWFERARRRIAVPARSMEEGER"
     gene            1137..2363
                     /locus_tag="VF058_05080"
     CDS             1137..2363
                     /locus_tag="VF058_05080"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014134790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX6207716.1"
                     /translation="MTVELPRPDPARPAIAGGPPAFPEGLPLVRPHRANVDRILQDIR
                     GSLEEGVLTNASLVRELERRAAEYLGVRHCIAVSSCTSGLMLALRAAGITGDVIVPSF
                     TFAATVHAVAWNGLRPVFADIDPETLTISPRAVRRAVGIRTGAILATHVYGTPCAVDE
                     LREIADHWGLRLLFDAAHAFGSRHSGTPVGGFGDAEVFSLSPTKVLVAGEGGIIATND
                     DALAERCRIGRDYGNPGDYDCLFVGLNARMSELHAAVALASLDGLDGRIDERNELAAA
                     YRQALGDLPGVTFPTVPDGERSTYKDFTILVDPGAFGLHPSGLATALEAEGIDTRRYY
                     APPVHRMQAYGGLADTNGGLPATDLASDRVLTLPLWNGMMEKDVHRVADAVGRIQRWV
                     PDRSEVVDLRTEVPGS"
     gene            2360..2665
                     /locus_tag="VF058_05085"
     CDS             2360..2665
                     /locus_tag="VF058_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207717.1"
                     /translation="MSDPEETMEAPLTINRAATAIVRSRIHLERAEEELRLALRIVTE
                     APVDATPLAPSERLARMQRLEGYLGRLRELQMMSRTVWDRDGSWWSDASVEGGTLGA"
     gene            2892..3575
                     /locus_tag="VF058_05090"
     CDS             2892..3575
                     /locus_tag="VF058_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6207718.1"
                     /translation="MAHIGAQALELERPFHGIRVLIANDQKLVAQAIESALSWEGFDV
                     VATVPPSHDGGFSAVREKRPELVILDLGRSGRDGIEWSKRILEEVPGVKILALIPIER
                     PVLGQAAMRSGIQGCLSKDSAIPDLINAISGVLAGHAVLPLPIARQMNGSRDVYVNLL
                     ADQLTQREIDVLELLVEGASSQEIMEELHVSRNTVRTHIQNILTKLQVHSRLEAAAFA
                     VRHGIVRTD"
     gene            3609..>3732
                     /locus_tag="VF058_05095"
     CDS             3609..>3732
                     /locus_tag="VF058_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEX6207719.1"
                     /translation="MSIELTEAAAQKVRELLDQEGAEAALRVAVQPGGCSGLRYS"
BASE COUNT          573 a         1282 c         1306 g          571 t
ORIGIN      
        1 tgaaccatta tgggcaccag ccccaaggtt gcaacgaccc ctgggggacg ccctgagagc
       61 ctacgacatc tgtggtagcc gaacatacgg tccctgaagc gtgggcgtgg ggccatatcg
      121 ccgtaccttc gaggagacgc aggagaggag ctgggtgcag tgatcgtgct ggtcacgggc
      181 gggacgggct tcatcggatc gaacctggtg cggtgcctcc tcggcgcggg tgcctcggtg
      241 cgggtgctcg acgacctctc cacggggcgg caggagaacc tcgacggtct caatgggggc
      301 ctcgacatcg tcgtggggga cgtgagggac ggcgagctgc tggcgtcggt ggtccgcggc
      361 accgaggtcg tctaccatct cgccgcgctc ccctcggtgg cccggtccat cgccgacccg
      421 gtcgccaccc acgacgtgaa cgcgacgggc acgctgaagg tcctgctgtc ggcgagggac
      481 gccggggcgc ggcgcgtcgt ctacgcatcc tcgtcctccg tctacgggga cacgccgacc
      541 ctgcccaagc acgagggcat gccggtcgcc ccgctgtctc cgtacgcggc ctccaagctc
      601 gccggcgagg cctactgccg gtccttcacc cgctccttca gcctcgagac cgtggcgctc
      661 cgcttcttca acgtgttcgg cccgcgccag gacccggctt cgcagtacgc ggccgtgatc
      721 cctcggttcg tgaaccaggt cctccaggga gagtcgccca ccgtgttcgg cgacggtgag
      781 cagagccggg acttcaccta catcgggaac gtggttcagg cgtgcgtgct ggccgccgag
      841 gccgccgccg aggcctcggg ccgggcgatg aacgtcgggt tcggccgacg cacgacgctc
      901 ctggagctga tccagatcct gggacggatc gtcggccggg agatcgagcc cgcctttgcc
      961 ccgcccaggc ccggcgacgt ccgggactcg ctggccgaca tctcgatggc ggagcgcctc
     1021 atcggatacc gaccgctgat ggaccttcgt gggggcctga ccgagaccgt ggagtggttc
     1081 gagcgggcgc gacgtcgcat tgccgtcccc gctcgttcga tggaggaggg agagcgatga
     1141 ccgttgaact tccccggccg gatccggcgc gaccggcgat cgcgggaggt ccccccgcct
     1201 tccccgaggg acttcccctg gtgcgccccc accgcgccaa cgtcgaccgg atcctgcagg
     1261 acatccgtgg aagcctcgag gagggggtcc tgacgaacgc gtccctggtt cgagagctcg
     1321 agcggcgggc ggccgagtac ctcggggtgc gccactgcat cgcggtctcg tcgtgcacga
     1381 gcggcctgat gcttgcgctt cgggcggcgg gcatcacggg cgacgtgatc gtcccctcct
     1441 tcacgttcgc ggccacggtg cacgcggtgg cctggaacgg cctccgtccc gtgttcgccg
     1501 acatcgatcc cgagacgctc acgatctcgc cccgcgcggt gcggcgggcg gtcgggatcc
     1561 gaaccggggc gatcctcgcc acccacgtct acggaacgcc gtgcgccgtc gacgagctgc
     1621 gcgagatcgc cgaccactgg ggcctccgcc tcctcttcga cgccgcccac gcgttcgggt
     1681 cccggcacag cggaacgccg gtgggcggct tcggcgacgc cgaggtcttc agcctgtcgc
     1741 ccaccaaggt gctggtggcg ggggaggggg gcatcatcgc cacgaacgac gatgccctcg
     1801 ccgagcggtg caggatcggg cgcgactacg ggaacccggg cgactacgac tgcctcttcg
     1861 tgggcctcaa cgccaggatg tccgagctcc acgcggccgt ggcgctggcc tcgctggacg
     1921 ggctcgacgg ccggatcgac gagcggaacg agctcgcggc cgcctaccgc caggctctcg
     1981 gcgacctccc gggcgtcacc ttcccgacgg tgccggacgg cgaacggagc acctacaagg
     2041 acttcacgat cctcgtggac cccggggcgt tcggcctcca tccctcgggc ctggccacgg
     2101 ccctcgaggc ggaggggatc gacaccagga ggtactacgc gccgcccgtg caccgcatgc
     2161 aggcgtacgg gggcctcgcg gacacgaacg gcggccttcc cgcgaccgac ttggccagcg
     2221 accgcgtcct gacccttccg ctgtggaacg ggatgatgga gaaggacgtg caccgggttg
     2281 ctgacgccgt ggggcgcatc cagcgctggg tcccggaccg atcggaggtc gtcgacctgc
     2341 gaacggaggt cccgggctca tgagcgatcc ggaggagacc atggaggccc ccctgaccat
     2401 caatcgggcg gcgacggcca tcgtccggag caggatccat ctggagaggg ccgaggagga
     2461 gcttcggctc gctctgcgga tcgtcaccga ggcccccgtc gacgccacgc cactcgcccc
     2521 gtccgagcgc ctggctcgga tgcagcgcct cgagggctac ctgggcagat tgcgtgagct
     2581 gcagatgatg tcgcgaaccg tgtgggatcg ggacgggtcg tggtggtcgg acgcctcggt
     2641 cgagggagga acgctcgggg cgtaggcaac aaccgaggag ggacacgacg aagcgggcgg
     2701 ggcatagtgc cccgcccgct tcgtcgtgtc cagacacgag tgccaccgtc atacgcctcc
     2761 gcgcctcatg catggtcagc agagggacat agtccatatg gatgaggcgt tgcgtcacgc
     2821 ataagaatca ccggttaatg gtcacaattg gggggttact cgaacgcggt ccgcgttcta
     2881 tcatcttcct catggcgcac atcggagccc aggccctcga gctggaacgg ccgttccacg
     2941 ggattcgcgt gttgatcgcg aacgaccaga agctcgtggc tcaggcgatc gagtcggccc
     3001 tgtcctggga ggggttcgac gtcgtggcca ccgtccctcc cagtcacgat ggaggcttct
     3061 cggccgttcg cgagaagcga cccgagctgg tcatcctcga cctcggacgc agcgggaggg
     3121 acgggatcga atggagcaag cggatccttg aggaggtccc gggcgtgaag atcctcgccc
     3181 tgatccccat cgagcgtccc gtactgggac aggccgcgat gcgctcgggg atccagggat
     3241 gcctgtcgaa ggactccgcg atccccgacc tcatcaacgc gatctcgggg gtcctcgccg
     3301 gccacgcggt cctcccgctt cccatcgccc gccagatgaa cgggagccgg gacgtctacg
     3361 tcaacctcct cgccgaccag ctcacccagc gggagatcga cgtcctcgag ctcctcgtgg
     3421 agggggccag cagccaggag atcatggagg agctgcacgt ctcccggaac accgtccgga
     3481 cccacatcca gaacatcctc acgaagctcc aggtccactc gcgactcgag gccgcggcct
     3541 tcgcggtgcg ccacggcatc gtgcggaccg actgacctgg ccgggtcccc gggtataatc
     3601 gtcaggtcat gtcgatcgag ctcacggaag cggcggcgca gaaggtcagg gagctcctgg
     3661 accaggaggg cgccgaggcg gctctgcgcg tggcggtcca gcccggcggg tgctcggggc
     3721 tccggtactc ga
//
LOCUS       DASUTW010000154         3631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_567022, whole genome shotgun sequence.
ACCESSION   DASUTW010000154 DASUTW010000000
VERSION     DASUTW010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567022"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VF058_05100"
     CDS             <1..240
                     /locus_tag="VF058_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207720.1"
                     /translation="LSFEIGWWLALAFFIVAAALAGWAMAAPRQPTEFAAPPPPATPP
                     GGTPPPPTGPPPGGAPPPPPEAPPPTRPQEPPPPA"
     gene            complement(244..1068)
                     /locus_tag="VF058_05105"
     CDS             complement(244..1068)
                     /locus_tag="VF058_05105"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX6207721.1"
                     /translation="MLRALILGAVQGLTEFLPISSSGHLVLVPFVLGWDGGTGFQRGV
                     SFDVAVPLGTALAVVVYFRRELWAIISGTVRVVARRDRGDDRDQARLALLLAVGSIPA
                     AAAGLLLEGFFVDLFEEPPIAALLLLVTAGLLLTGEAIHDRQGEPRRGLADLRMADAL
                     TVGAFQALAIAPGISRSGATIVAGLGRRLERDAAARFSFLLALPAIVGAGILKIPDFP
                     AGTDLGPVIGAMIVSAGAGFAAIAFLLRYLRTRTLRPFAVYCVLASAVALTVWLVR"
     gene            complement(1078..1716)
                     /gene="cpaB"
                     /locus_tag="VF058_05110"
     CDS             complement(1078..1716)
                     /gene="cpaB"
                     /locus_tag="VF058_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX6207722.1"
                     /translation="MRRKLPRSAVALALAAVVVGAGTTVALRGRLAALEARAGAAGDL
                     RLAVVAAADLPRGARIEPGHLETVEMPAALLPSTALTSERQAIGATLAGDVAEGEVLT
                     EARVARAGPIAALVPEGFRAMGVTVAIAPGLLAPGDRVDVLAVTPGRPFAEPLASGAE
                     VLAVRETSLAEASVATVVLLVTSGQASELAAGRAGAELTLTVLPAGEVTMSG"
     gene            complement(1785..2579)
                     /locus_tag="VF058_05115"
     CDS             complement(1785..2579)
                     /locus_tag="VF058_05115"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX6207723.1"
                     /translation="MSTIGIFGGSGFYSLLEDAREVEVDTPYGKPSAPFTVGEIGGRE
                     VAFLPRHGRKHQFPPHKVPYRANLWGMKELGVERVVGPCAAGSLQAHVKPGHFVLCDQ
                     LVDRTSGRADTFYDGPETTHVSFADPYCPTMREVAFEQARDQGIEIHDRGTVVVIQGP
                     RFSTRAESAWFQSEGWEVINMTQYPEAVLARELELCFLNISLITDYDVGVEGEDVEPV
                     THEAVIQVFEENIEKLKELLHRIVPELPEARDCPCATALEGARFEP"
     gene            complement(2576..2842)
                     /locus_tag="VF058_05120"
     CDS             complement(2576..2842)
                     /locus_tag="VF058_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013127857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX6207724.1"
                     /translation="MPTYEYECRECGHRFEVNQRISDDPLVECEVCGGPLRKLFHPVG
                     IAFRGSGFYVTDNRGSRAKAPSSDGKKPEKKAESATSESKEKTA"
     gene            complement(2876..3451)
                     /locus_tag="VF058_05125"
     CDS             complement(2876..3451)
                     /locus_tag="VF058_05125"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX6207725.1"
                     /translation="MTDDPVKATLRAEMRRVRAAIPEGERRRRGEAIRKRVLGLPEVR
                     RAGSVLVFASFGSEVPTRDLASDLHAAGKRLLLPYLSGEVMEVAEVGPDERLAPSSYG
                     PGEPERRVRADPAGIDVVIAPGLAFDRRGNRLGYGGGAYDRFLARVRPEALVVGVAFA
                     EQVVEEVPAGPSDVPVHLMVTDREVIDARRE"
BASE COUNT          527 a         1410 c         1237 g          457 t
ORIGIN      
        1 ctgagcttcg agatcgggtg gtggctggcc ctggccttct tcatcgtcgc ggccgcgctc
       61 gcggggtggg ccatggctgc cccgaggcag ccgacggagt tcgcggcgcc gcccccgccc
      121 gcgacgcctc cgggagggac tccgccgccg ccgaccgggc cgccgccggg tggggctccg
      181 cctccgcccc cggaagcccc gccccccacg aggccccagg agccgccgcc gccggcctga
      241 gcgtcagcgc acgagccaga ccgtgagggc gacggccgag gccaggacgc agtagacggc
      301 gaagggtcgg agggtcctcg tccgcaggta gcggagaagg aaggcgatcg cggcgaaccc
      361 ggccccggcg gagacgatca tcgcgccgat caccggcccg aggtcggtcc ccgccgggaa
      421 gtcggggatc ttcaggatgc ccgcccccac gatggcgggc agcgccagga ggaacgagaa
      481 gcgggcggcc gcgtcccgct ccagccgccg cccgaggccc gccacgatcg tggcgcccga
      541 gcggctgatg ccgggagcga tggccagcgc ctggaaggcc cccaccgtga gcgcgtcggc
      601 catccgcagg tcggccagcc ccctccgcgg ctccccctgc cgatcgtgga tcgcctcgcc
      661 cgtcagcagg agccccgccg tgaccaggag gagcagggcc gcgatcggcg gctcctcgaa
      721 caggtcgacg aagaagccct ccaggaggag gcccgccgcg gccgcgggga tcgaccccac
      781 cgccagcagg agggccagcc gcgcctggtc ccggtcgtcc ccgcggtcgc ggcgggccac
      841 cacccgcacg gttcccgaga tgatggccca cagctcccgc cggaagtaga cgacgaccgc
      901 cagggcggtc cccagaggaa cggcgacgtc gaaggacacc cctcgctgga accccgtccc
      961 cccgtcccat cccaggacga agggcacgag cacgaggtgt ccggaggacg agatcgggag
     1021 gaactccgtc agcccctgga ccgcccccag gatcagggct cgaagcatgc ggggagtcta
     1081 gccgctcatg gtgacctccc ccgccggcag caccgtcagg gtcaactcgg ccccggcccg
     1141 cccggccgca agctcggagg cctgccccga cgtgacgagc agcaccaccg tcgccacgga
     1201 cgcttcggcc agcgacgtct cccggaccgc cagcacctct gcccccgagg ccagcggctc
     1261 cgcgaacggc cttcccggag tgacggccag cacgtccacg cggtcgccgg gggcgagcag
     1321 accgggcgcg atcgccaccg tcactcccat cgctcggaac ccttcgggga cgagcgcggc
     1381 gatcgggccg gcgcgggcga cccgggcctc cgtcaggacc tcgccctcgg ccacgtcgcc
     1441 cgcgagcgtc gccccgatcg cctgacgctc ggacgtcagc gcggtggatg ggaggagcgc
     1501 cgcgggcatc tccacggtct ccaagtgtcc gggctcgatc cgggctcccc gaggcaggtc
     1561 cgcagcggcc accaccgcca gccgcaggtc gccggcggct ccggctctcg cctcgagggc
     1621 ggccagccgt cctctcagcg cgaccgtggt ccccgccccg accaccaccg ccgccagcgc
     1681 gagcgcgacg gccgaccggg ggagcttcct gcgcatgagc cctcctccga gggggaggct
     1741 gggagacgtc cggggagcct agcgcccccc accgacacga acgcctatgg ctcgaacctg
     1801 gcgccctcga gcgcggtggc gcaggggcag tcgcgggcct cggggagctc gggcacgatc
     1861 cggtgcagca gctccttcag cttctcgatg ttctcctcga acacctggat cacggcctca
     1921 tgggtcacgg gctcgacgtc ctcgccctcc actcccacgt cgtagtcggt gatcagcgag
     1981 atgttgagga agcagagctc cagctcacgg gccagcacgg cctcggggta ctgggtcatg
     2041 ttgatgacct cccagccctc ggactggaac caggcggact ccgcgcgcgt ggagaagcgg
     2101 ggcccctgga tcaccaccac cgtgccccgg tcgtggatct cgatcccttg atcccgcgct
     2161 tgctcgaacg ccacctccct catcgtcggg cagtaggggt ccgcgaacga gacatgggtc
     2221 gtctcgggcc cgtcgtagaa ggtgtccgcc cgcccgctgg tccggtccac gagctggtcg
     2281 cacagcacga agtggcccgg cttcacgtgc gcctggagcg agcccgccgc gcacggtccc
     2341 accacccgtt ccacccccag ctccttcatc ccccagaggt tcgcccggta cggcaccttg
     2401 tggggcggga attggtgctt ccgcccgtgc cgagggagga acgcgacctc gcgccccccg
     2461 atctcgccca ccgtgaacgg ggccgagggc ttcccgtagg gggtgtccac ctcgacctcg
     2521 cgagcatcct ccaggagcga gtagaacccg gatcccccga agatcccgat cgtgctcagg
     2581 ccgtcttctc cttcgactcc gaggtcgcgc tctcggcctt cttctcgggc ttcttgccgt
     2641 cgctcgaggg cgccttcgcc cgcgacccac ggttgtcggt cacgtagaac cccgagcccc
     2701 tgaaggcgat gccgaccggg tggaagagct tccgcagagg tccgccgcag acctcgcact
     2761 cgacgagcgg atcgtccgag atgcgctggt tcacctcgaa gcggtgaccg cactccctgc
     2821 actcgtactc gtaggtcggc atggcccagc gattataggg ggcggagcga gccgctcact
     2881 cgcgccgggc gtcgatcacc tcgcgatcgg tcaccatgag atgcacgggg acgtcgctgg
     2941 ggccggccgg cacctcctcg acgacctgct ccgcgaaggc cacacccacc accagggcct
     3001 ccggacggac ccgggccagg aagcggtcgt acgcgccgcc cccgtacccc agccggttcc
     3061 ccctgcggtc gaacgccagc cccggggcga tcacgacgtc gatccccgcc gggtcggcgc
     3121 gcaccctccg ctcgggctca cccgggccgt aggagctcgg ggcgagccgc tcgtccggac
     3181 ccacctccgc gacttccatc acttccccgg agaggtaggg taggagcagg cgcttcccgg
     3241 ccgcgtggag atcggaggcg aggtcccggg tcggcacctc cgacccgaac gaggcgaaga
     3301 cgagaacgga tccggcccgc cggacctcgg gcaggccgag gacccgcttt cggatcgcct
     3361 ctccccgccg acgacgctcg ccctccggga tcgccgcccg cacgcggcgc atctccgcac
     3421 ggagggtggc cttgacggga tcgtcggtca cggtcgctcc tgattaagcc gtgggtgagc
     3481 gggtgccgac aatgaagggg agacaaccga tgcgcgcgcg tcgctcatcc cgggaagcca
     3541 acgcgcgagg ggcccagccg tgaccctgcg cgctcgcctc acgctgttct tcgccggcat
     3601 cgtcgtcgtc cccctcgtcg cggccacgct c
//
LOCUS       DASUTW010000155        20332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1603673, whole genome shotgun sequence.
ACCESSION   DASUTW010000155 DASUTW010000000
VERSION     DASUTW010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 20332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20332
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603673"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..807)
                     /locus_tag="VF058_05130"
     CDS             complement(91..807)
                     /locus_tag="VF058_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX6207726.1"
                     /translation="MALVLGAAACAPSEPAAENVRLGSEESSPGSGSGSGSSQSSPKR
                     VDPRRDGFEVGLGEWAVTLEAAAIRPGPVTFVVHNGGTMVHGFEMELENVDDGFKVET
                     ETFGPGETVTLDLDLAPGLYKVECLIDGHDDLGMEALLEVRPDAPKVPQSRPSADASA
                     VSIRGFAFDPSELSVDSGRELTWTNDDPAPHTVTARDGSFDSGTIEPGQSFSVTVDAS
                     GAVTYFCEIHPTMEGTIRVG"
     gene            complement(831..1178)
                     /locus_tag="VF058_05135"
     CDS             complement(831..1178)
                     /locus_tag="VF058_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX6207727.1"
                     /translation="MRYLTRTVVVVGALLALTACGGDGDDAGGGPGEASLSMVENAFQ
                     PADLTVAAGGELSVSNDGQAVHNITIEGTSIDEDVDAGQSTTITIDAEPGEYTMFCGF
                     HRAAGMEGTVTVQ"
     gene            1298..1370
                     /locus_tag="VF058_05140"
     tRNA            1298..1370
                     /locus_tag="VF058_05140"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1331..1333,aa:Ala,seq:tgc)
     gene            complement(1407..1835)
                     /locus_tag="VF058_05145"
     CDS             complement(1407..1835)
                     /locus_tag="VF058_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6207728.1"
                     /translation="MKPGIRPATAGDAEAVASLATQLGYPATADEIVERLAGLPSHHT
                     VFVAEMEGAVVGWIHVQESRLVVERLRADITGLVVEEAARRGGVGRLLVEAAERWAAD
                     RGADRIRVRSNVIREDAHAFYPSLGFEIAKTSTVFEKDLG"
     gene            complement(1832..2815)
                     /locus_tag="VF058_05150"
     CDS             complement(1832..2815)
                     /locus_tag="VF058_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002721019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEX6207729.1"
                     /translation="MRDIGLAGLAGAGKSTLFTALTHTGTAGGRSNQAVVPVPDPRLE
                     VLAEIEGSARIVPAQVRFVDVPGGLSSQAIVTLRQADALAIVLAAFHAAADPPADLGT
                     IRAELMLADLANVETGLERAERKAKGRDEEALADLDRLSRARALLEGERPLREGEWSD
                     DDLMAMRGYGFLTLKPWIVVANLEEGADLPPGLPEGAVAVYAAIEAEVAAMDPEEGAA
                     LLREFGVEEPGLQKLIRAAYDALDLITFLTTGEDETRAWQVRRGALAPEAAGQIHTDL
                     ERGFIRAEVVAYEDLVAAGSMKAAKDRGLVRTEGKAYEVQEGDVLNILFAV"
     gene            2877..3254
                     /locus_tag="VF058_05155"
     CDS             2877..3254
                     /locus_tag="VF058_05155"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HEX6207730.1"
                     /translation="MAERSDLQTPEEYFEGHPEALKIFRWVRRTVEATGPATIGVSRS
                     EVAFRRRRGFAWLWMPGRWLRDPAAEVVLSFALPRRDRSPRLKEVVEPRPGLWMHHLE
                     VHDPDEMDDEVSEWLREAFEAAS"
     gene            3251..3400
                     /locus_tag="VF058_05160"
     CDS             3251..3400
                     /locus_tag="VF058_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207731.1"
                     /translation="MTDGEAREAAFCEPESKRLPCPVCGEAMEPEHAHYRCPTCRYIE
                     PCCGW"
     gene            complement(3402..3641)
                     /locus_tag="VF058_05165"
     CDS             complement(3402..3641)
                     /locus_tag="VF058_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207732.1"
                     /translation="MGASCMMCGRATYDPDKRERPWARGVAGGRLVLICPRCQTERPD
                     WPRRLDTCGACGSTRLSITLGEVICRACDHSTPAA"
     gene            3673..4140
                     /locus_tag="VF058_05170"
     CDS             3673..4140
                     /locus_tag="VF058_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207733.1"
                     /translation="MTTLPGTTLARGEVLPGTPLIDAATGGEVRVWDFRGRTALVLCF
                     LHLACEACERFAAGLAEAESEIRLTGACAFAVLREPAPSALPVFVDGDGDASRRFLGP
                     EGALPTILVADRYAAAWESYPVLGHDFPPASEIVGDLWHLATMCEECGIMAWE"
     gene            complement(4142..5476)
                     /locus_tag="VF058_05175"
     CDS             complement(4142..5476)
                     /locus_tag="VF058_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6207734.1"
                     /translation="MGRRALLVAGLLVAVMSAADLAVASTGEEEPPPIEIDITALPNP
                     FSPNGDGRRDEAVIAVTSSAAGSVTVSIQDTAGETWRAWPELLLPEPGSVEVVWRGRS
                     GEVRAPDGRYEVVAVIATEEGATATGATRLVLDRQPPRLRRLRVRPDPLTTHRWLVIR
                     FMGRDRAPRIRTELRIEDALGTIGKQAVQKPPGPTRVRWRARYRSGKRLYPGNYRLGV
                     RLWDDAGNVSRRRWVPWRVHRNVGGRVYRRLEGTGRRVALTFDDCGNTSAWGSILRTL
                     HAKNVEAAFFCGGGAVRGNPSLARRTVRDGHTPGSHALDHAILTGHDPSFTSSRVRAD
                     RRAWWQVARRTSAPYLRPPYGACNASVVEGAAAAAAPRVVMWDVDPQDWRSPGVSEIR
                     RRVLSAARPGSIILLHTGDQTAASIGSIVDGLRARNLEPVGLSALFRAAGMH"
     gene            6056..7237
                     /locus_tag="VF058_05180"
     CDS             6056..7237
                     /locus_tag="VF058_05180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013987393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX6207735.1"
                     /translation="MREAVIVEAVRSPVGRRGGKLSGWHPVDLLAEVLSAALERSGID
                     PPRVEDHISGCVSQTGEQAINIARNAWLAAGLPEEVPSVTVDRQCGSSQQAAHFAAQG
                     VMAGAYDLVIASGIEHMTRVPMGVTMEQGPGFPFSQRLLDRYDGRLVPQGVSAEIIAE
                     RWNLSRGELDEFSARSHELAAKAIKDGRFQDEIHPIEVGTEGETELFEHDEGVRFEVD
                     LDKMATLPTPFKEDGVVTAGNSSQISDGAAAIVIASREKAEELGLRPRARFHAFSLSG
                     VDPITMLTGPIEATRKVLASGGLSLEDVDVLEINEAFASVVLAWKQELEIPDDWFEAR
                     VNSNGGAIALGHPLGASGGRLMTTMLHELERREGRYGLQVMCEGGGLANATIIERLVV
                     D"
     gene            7242..7493
                     /locus_tag="VF058_05185"
     CDS             7242..7493
                     /locus_tag="VF058_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207736.1"
                     /translation="MRLAIAILLAVGIAGIGVWILSAIGRNRDLVREVPVEDVEELDV
                     YFVCGECGTEFRVEKLGELQVPRHCGETMRVEQRPAPLA"
     gene            complement(7490..7744)
                     /locus_tag="VF058_05190"
     CDS             complement(7490..7744)
                     /locus_tag="VF058_05190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX6207737.1"
                     /translation="MPVYEFRCEDCGASLELVRPMGRTEPPGSCPACGGSRMRRRWSR
                     VGVRFEGWGFSRTDALLPEDRRARRDYKDLKRKADEIAES"
     gene            complement(7823..8056)
                     /locus_tag="VF058_05195"
     CDS             complement(7823..8056)
                     /locus_tag="VF058_05195"
                     /inference="COORDINATES: protein motif:HMM:NF018487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HEX6207738.1"
                     /translation="MPKSKSKRRRYQPPKPPKPKPSPTWFGILVLTLLFVGVLMIVLN
                     YLGFLLPGSPNNTYLWVGLGLIAVGFALATQWR"
     gene            8096..8803
                     /locus_tag="VF058_05200"
     CDS             8096..8803
                     /locus_tag="VF058_05200"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEX6207739.1"
                     /translation="MDRPGSRFAMPLAAGVLGFLAVLAAAQPQAVPGRESSRLEIVDL
                     IEQQDGRVRQLRQEVGDLEARIREASSGETSAAIGELRAEAEALAVAAGAAPLRGPGI
                     TVVLDDSSASRSATGDPNDLIVHERDLQTVVNALWAGGAEAVAINGERLSSLSAVRCA
                     GNTLLLHGVVHSPPYEIRAIGDPEALERSLEDSPGMRRLHEAVRAFGLALEIERGSLV
                     VPVGRGVRELATAAPAA"
     gene            8843..9391
                     /locus_tag="VF058_05205"
     CDS             8843..9391
                     /locus_tag="VF058_05205"
                     /EC_number="3.4.22.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEX6207740.1"
                     /translation="MALFVVYELFVTDVITGRHQDALRQDLGRRLQVAGPNRDVRPVP
                     GRGVAILRIPRIDLDAVVVEGVGLEDLKKGPGHYPRTPMPGDGGNVGIAGHRTTYGKP
                     FWSLNELTPGDEIFLRTSGGEFAYEVLWTRVVLPSQSEVLDRTRGPSLTLTTCEPRFS
                     AARRLIVRAVQVDPPPDPEAPG"
     gene            9388..9540
                     /locus_tag="VF058_05210"
     CDS             9388..9540
                     /locus_tag="VF058_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207741.1"
                     /translation="MIRFLRRRSPGNPLLAVLYWVVLAATVVILVFALFYLLDVSRFV
                     PGGGMF"
     gene            9550..10263
                     /locus_tag="VF058_05215"
     CDS             9550..10263
                     /locus_tag="VF058_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEX6207742.1"
                     /translation="MTEEIAATDAYARETRATVVGSGDDGVVLDRTVFYARGGGQPGD
                     TGALRWDGGEVRVVDTVRREGRVLHEVEGDAPAPGTAVTATIDWERRHRLMRIHTALH
                     ALSGLIWRDYDAKVTGGNMDEGAARMDFELGEMSAEFGREVEEKLNAALAEDRPVHVR
                     FLPRAEALQDPDLIRTKVNLIPDSVDPIRVIDIEGIDRQADGGTHVRSTGEVGRVRVT
                     KTESKGKAFKRIRIALDQG"
     gene            complement(10260..12599)
                     /locus_tag="VF058_05220"
     CDS             complement(10260..12599)
                     /locus_tag="VF058_05220"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX6207743.1"
                     /translation="MDDPIEALRGAARAALPPVEGEVRLPGLGAAVEIRRDRWGVPHI
                     YADHLTDLWTAQSYVMASERLFQIELAYRLGLGRLSELFGELTLSMDRFVRTLGWNRA
                     AAEHAAAWDDLSLEMAAAGAAGARAWLEAMPAKPVEYVVLGVDPWLPDQDEAALAGAG
                     AAVYVAWSLSRAWDNDLLRAEIAERFGVEAMRALFPDALPEPEAVRAAKEATDPRLAM
                     LRNAILAPSGEGSNAWVVASSRSATGAPLLANDPHLVVQAPSAWFEAHLVGPGVDVAG
                     VTFPFTTGIVIGHNDRIAWGFTNSEGDVQDLYLERLSEDGASAKHLDDWEPVTVVREE
                     IGVRGRAEPEVLEVRETRHGPLVGSYMVGIASPRVIQGGIRRPYALRWVGAEGAIEPS
                     TIHRLDTARSWEEFRAAVARWHCPGQNMVYADVEGNIGYQLTGRYPARRAGDGSLPVP
                     GWTDEHEWDGWIPFDELPRAFNPEAGFVVTANNPPGGDGQSSLGVDFLPPYRARRIAQ
                     LLTARPRHDRESFAAIQADTRSLLAPEILRHLLAIEARDERQTEALGLMIEWDQDLGR
                     DSAAAAIYESWCTHLAARILRPRLGRELFEHYFARRQWTNGFHYRALPELLAYPTARW
                     FGEDGAAARDDVVRAALDEALTELTDRLGEPMTDWRWGAVHRARFAGRLAAAPGLGEL
                     FTVGEVELGGDEQTVAQAHFEPGAPYEAVVVPSWRQIVDLWDLDASVGVLTLGQSGNP
                     ESPHYRDQLPLWASGEHHPMPFTRDAVERATASVLRLVP"
     gene            12706..14466
                     /locus_tag="VF058_05225"
     CDS             12706..14466
                     /locus_tag="VF058_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX6207744.1"
                     /translation="MANDRTERPRPWLDLYAQGVPHTLQPYPEMSAFEMLERTVQRFP
                     DSPAVAFPVAPMARTLTYRQLLDEVEQMSRALASLGVKRGDRVGLLLPNSPHFVVAHF
                     AIQRLGAVTVGNNPLYTKRELVHQLNDAGVEILIALDLLYPLVEAAQTEVAFRKVIVG
                     KVGDYLGFPINKLAPLKQRREAKHEGRRWPPVPQDAEVTWWADLMKGSYPDVPPAQVD
                     PKEDVAALVYTGGTTGLSKGAMLTHLNIVANAAQTAAWFTGIEEGHEGIVAVLPFFHS
                     YGLGAIMGVGVYKGLKLVLLPRFELDLVLKAVEKERPTLFPGVPRIFIAINESPETAK
                     HDISSIRSCFSGAAPLPVAVAERFEQITGGRLVEGYGLTETSPVTHINPLDGSHRFGT
                     IGLPIPDTDCKLVDIDEPLKEVGGDQEGELCVAGPQIMKAYWNRPEETEDMIRVHPDG
                     IRWLHTGDIAKMDPDGFFRIVDRKKDIIIVSGFNVYPTDVEQVLYRHPKIEKVSVVGV
                     PDETTGEAVKAFVILKAGQEATEDEIKAWARDPEHGLTGYRAPRHVEFRDELPETMVG
                     KVLRRKLQEEEKEKASAQES"
     gene            complement(14454..14576)
                     /locus_tag="VF058_05230"
     CDS             complement(14454..14576)
                     /locus_tag="VF058_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207745.1"
                     /translation="MTKQDWLFLALGTLALASLLTSIGTWMVLWDRLRSRRYDS"
     gene            complement(14573..16177)
                     /locus_tag="VF058_05235"
     CDS             complement(14573..16177)
                     /locus_tag="VF058_05235"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX6207746.1"
                     /translation="MAPEPPWVEETFTDEERERLAPFVTSLEGPVFALVNLPEVTKGA
                     LFARYSRSSKSVRRLLLDEFLEEAEGGAGAGEAGVARAERLYENVFFEYGDDSVAQLG
                     GVHLACEQSSQILAKVLEWGRLAAYLEQSTRYVPYDDRPGGRYRYAIPPEVEAAGLSA
                     RYSEVLDHAFDTYARWLEPMQELYRARHPQDAGDPDFVYRSTIRAKACDTLRGLLPAA
                     TRTNVGIYATGQAYEQLLLRMRGHPYAEVRAYADLMLAELRKVIPAFLRRVDLPDRGG
                     AWSDYLARTREETAKVAEELVGDLQAPPRPEVALTDSDPEGEVKVVAAALYAATGLPD
                     DVLLERARAMSTEQRDRVLTAYVGDRPNRRHKPGRAFERAGYRFDVLCDYGAFRDLQR
                     HRMLTIEWQELTTAHGFERPNDVEEAGAREDWDRVMEASAALEGELRAAGLGGVAQYA
                     VAMAYRIRFVMQMNAREAMHLIELRSSPQGHPAYRRVAHEMHRLIRDEAGHRAIAGSM
                     RHVDLSEVDLERLEAERRAASRRGSA"
     gene            complement(16167..17852)
                     /gene="pknB"
                     /locus_tag="VF058_05240"
     CDS             complement(16167..17852)
                     /gene="pknB"
                     /locus_tag="VF058_05240"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEX6207747.1"
                     /translation="MAKQRVLGNRYEIERVLGEGGMAKVFLGTDRVLGRTVAVKVLSP
                     QYAEDQQFVARFRREAQAAAALNHPNIVSVFDTGSTGNVHYIVMEYVEGRTLQDTLRE
                     ESRLTPERSIEIGEAVAKALSAAHAKGLVHRDIKPGNIMITREGQVKVMDFGIARATT
                     SDTVTQTAAVLGTAAYLSPEQAQGETVDHRSDIYSLGVVLYEMLTGRQPFTGDSPVSI
                     AYRHVTEDPTPPSRLNPDVRESLDAIVMTSMAKAPGDRYQSAQELIDDLRRASEGMPV
                     AAAVPLPGDTTQVIDRGGHTQVLTGPLEEEMEEEEERGRRVGWIVFWTLLILAILGLA
                     SFFLVRALLTGPEPVTVPDVVGFNRVAAVQTLEEAGLEVEVVRRARDDVPADQVFRQD
                     PEAGTEVDEGSLVRIFVSRGTRMVEVPSVVGLSQEEAQQAIEDAGLTLGFVGQEPSEE
                     DPGIVISQDPQPETEVEEGSAVNIVISTGPETVAVPSVVCDSPEDARSEIEGRDLVYQ
                     EETEEFSDCPSGTVARQDPEAGAEVAPGSTVRVWISLGPSPEPSPTDSPTDGA"
     gene            complement(17898..19358)
                     /locus_tag="VF058_05245"
     CDS             complement(17898..19358)
                     /locus_tag="VF058_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX6207748.1"
                     /translation="MTTVLADRYELEEPLGAGGMASVYRGTDRVLDRTVAVKVLDRSL
                     GRDPEFVERFRREARAAAGLTHPGTVAVYDTGEDDGRRFIVMELIDGETLADLLAREA
                     PLDPERVALLAADILEALAAAHDRGLVHRDVKPGNVLLTSEGEPKVADFGIARAATSE
                     TITVGSKVLGTAAYLSPEQARGRPVDARCDLYAMGCVLYEMLTGAPPFRGGSPVSVAT
                     KHIHEDPPPLPSSVPPSLAAVVMQALEKDPDDRFPDARSMAAAIRGGAGGAGDTMVLE
                     EEALETEAVPAPPAPLRRRWVLPALLGALLLAALIGGLVLAFGRDDPTAASRGDRDRG
                     AAAAGPSPAESPREEAEQLRPQEAFTALVGAVQDGLTAGEIEEKLAREIVERAEDAME
                     AYAEGDRQKALDKAGDAINALVDGAEQGEVTPERASEIEHRILALQAAIEAYPPREHE
                     EEDDDEDEDGSGGGGPDGEGPPGPDGEGPPGQDDDT"
     gene            complement(19355..>20332)
                     /locus_tag="VF058_05250"
     CDS             complement(19355..>20332)
                     /locus_tag="VF058_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX6207749.1"
                     /translation="RTGEVLALVGLPTFDPNRLSSHDGREVRRAWNQLNDDPDKPMLS
                     NANDEVFPPGSTFKVVTAAAALENGLGPESTWDNPPSLDLPQTTNNLDNFGGSHCLGG
                     ASQITLAQALQVSCNVTFGQVGLEVGAEALMEQARRFGFSEDVPFDIPFAEGQVPDPS
                     AFDQDLPGLAFSAIGQQDVRTNPLHMALVAGSIGNGGVMMRPRLVVEIRDPSGRVIER
                     FDPEVYGRPLSGGNARALTQMMVSVVSSGTATGAQIPGVSVAGKTGTAQTAEGQPPHA
                     WFIAFAPAEQPSVAVVVLGRGNLSSEATGGAIAAPIAKAVMEAALEGGS"
BASE COUNT         2874 a         7191 c         7328 g         2939 t
ORIGIN      
        1 cggtacggaa ggattccgta ccggccgcgc ccccccggcc gtccatcacc ctgtagacgc
       61 ccaccggcat gccgtcagta tgccgcgacg tcacccgacg cggatcgtcc cctccatcgt
      121 gggatggatc tcgcagaagt aggtgaccgc ccccgaggcg tccaccgtca ccgagaacga
      181 ctgccccggc tcgatcgtgc ccgagtcgaa ggacccgtcc ctcgcggtca ccgtgtgggg
      241 ggccgggtcg tcgttggtcc aggtgagctc ccggccggag tcgaccgaga gctcggacgg
      301 gtcgaaggcg aagccccgga tggagacggc gctcgcatcg gccgaaggcc gagattgcgg
      361 caccttcggg gcgtcgggac gcacctcgag gagggcctcc atgcccaggt cgtcgtggcc
      421 gtcgatcagg cactcgacct tgtagaggcc tggggcgaga tccaggtcga gcgtgacggt
      481 ctcgcccggg ccgaacgtct ccgtctcgac cttgaacccg tcgtcgacgt tctcgagctc
      541 catctcgaag ccgtgcacca tcgtcccgcc gttgtggacc acgaacgtga ccggtccggg
      601 gcggatcgcc gccgcctcca gggtcacagc ccactcaccg aggccgacct cgaagccgtc
      661 ccgccggggg tcgacccgct tcggagagct ctgggacgaa cccgatccgg atcccgaccc
      721 cggggagctc tcctcgctcc ccagccggac gttctcggcg gccggctcgg agggggcgca
      781 ggcggccgcc ccgagcacga gtgccatgcc gatgatcgcg agcgagcgcc tcactggacc
      841 gtcaccgtgc cctccatccc ggccgcgcgg tggaacccgc agaacatcgt gtactcgccg
      901 ggctccgcgt cgatcgtgat agtcgtgctc tggccggcgt cgacgtcctc gtcgatcgag
      961 gtgccctcga tcgtgatgtt gtggaccgcc tggccgtcgt tcgagacgga gagttcgcct
     1021 cctgccgcca ccgtgaggtc ggccggttgg aaggcgttct ccaccatcga gagcgaggcc
     1081 tcgcccgggc cgccgcccgc gtcatcgccg tcgccgccgc aggcggtgag ggccagcagc
     1141 gccccgacca ccaccaccgt tctcgtcaga tacctcatcc gtgcctcctt ctcgtcagac
     1201 acggtgatcg tcgcccccgg gcatggacct ctcctgaagc ggccctgaag gttccttcag
     1261 gttggcccat ctcgtcgggg gctagactag tgggaacggg gacgtagctc agttggtaga
     1321 gcgcctgctt tgcaagcagg aggtcgtcgg ttcgaatccg atcgtctcca ccgggaaggt
     1381 ttcctacggg aaggcagagc gagcagtcat cccaggtctt tctcgaagac cgtggaggtc
     1441 ttcgcgatct cgaatcccag cgacgggtag aaggcgtggg cgtcctcgcg gatcacgttg
     1501 gaacggaccc ggatccggtc ggcaccgcga tcggccgccc accgctcggc cgcttccacg
     1561 agcagccgcc ccacgcctcc gcggcgggcc gcttcctcca cgacgaggcc ggtgatgtct
     1621 gcccggaggc gctccacgac caagcgggac tcctggacgt ggatccaccc cacgaccgcg
     1681 ccctccatct cggccacgaa cacggtgtgg tgggaaggca gcccggccaa acgctccacg
     1741 atctcgtccg cggtcgccgg gtagccgagc tgcgtcgcga ggctcgctac cgcctccgca
     1801 tcgcctgcgg tggcgggccg gatcccgggc ttcacaccgc gaagaggatg ttgagcacgt
     1861 ccccctcctg cacctcgtag gccttgccct cggtccggac gaggccgcgg tccttggccg
     1921 ccttcatcga cccagccgcg accagatcct cgtaggccac gacctcggcc cggatgaagc
     1981 cgcgctcgag gtccgtgtgg atctgcccgg ccgcctcggg cgcgagcgcc ccccgacgaa
     2041 cctgccaggc ccgcgtctcg tcctctccgg tcgtgaggaa cgtgatgaga tcgagcgcgt
     2101 cgtacgcggc ccggatcagc ttctgaaggc cgggctcctc gaccccgaac tcccggagga
     2161 gggctgcgcc ttcctcgggg tccatcgcgg ccacctcggc ctcgatcgcg gcgtaaacgg
     2221 cgacggctcc ctccgggaga cccgggggca ggtcggcccc ttcctccagg ttggccacca
     2281 cgatccacgg cttcagcgtg aggaacccgt atccacgcat cgccatcagg tcgtcgtccg
     2341 accattcccc ctcgcgcagc ggacgctctc cctcgaggag ggcgcgggcc cgcgagagac
     2401 ggtcgaggtc agcgagcgcc tcctcgtccc tccccttggc cttacgctcg gcgcgctcca
     2461 ggccggtctc gacgttggcc agatcggcca gcatgagctc ggcgcggatc gtcccgaggt
     2521 cggccggggg atccgccgcg gcgtggaagg cggccagcac gatcgcgagg gcgtcggcct
     2581 ggcggagcgt gacgatcgcc tgggacgaga gcccgccggg gacgtcgacg aatcgcacct
     2641 gagccgggac gatccgcgcc gacccctcga tctcggccag gacctccagc cgcgggtcgg
     2701 gcaccgggac gacggcctgg ttcgagcgtc caccggcggt gcccgtatgg gtcagggcgg
     2761 tgaacagggt ggacttcccg gcgccggcca gtccggccag tccgatgtcc ctcatcggtc
     2821 gcctacgcta ccaagggtgc gtgatccggg cggtgacgcc gggtaggttc gggcggatgg
     2881 ccgagcgttc tgacctgcag acccccgagg agtacttcga ggggcatccg gaggcgctga
     2941 agatcttccg ctgggtccgc aggacggtcg aggcgaccgg cccggcgacg atcggcgtct
     3001 cgaggagcga ggtcgccttt cgcaggcgcc gagggttcgc gtggctctgg atgcccgggc
     3061 gctggctccg cgatccggcg gcagaggtcg tgctctcgtt cgcgctcccg aggcgcgacc
     3121 ggtctccccg actcaaggag gtggtggagc ctaggcccgg actctggatg catcacctgg
     3181 aggtgcacga tccagatgag atggacgacg aggtgagcga gtggctgagg gaagcgttcg
     3241 aagcggcctc gtgacggacg gggaggcgag agaggccgcc ttctgcgagc ctgagtcgaa
     3301 gcgcctcccg tgccccgtgt gcggcgaggc gatggagccg gagcacgccc actaccggtg
     3361 cccgacgtgt cgctacatcg agccgtgctg cggctggtag gtcacgcggc gggagtcgag
     3421 tggtcgcagg cccggcagat cacctcgccc aacgtgatcg agaggcgggt gcttccgcac
     3481 gcgccgcacg tgtcgaggcg gcggggccag tccgggcgct cggtctgaca gcgcgggcag
     3541 atgagcacga gccggccgcc tgccacaccc ctcgcccacg gacgctcgcg cttgtcggga
     3601 tcgtaggtcg cccggccgca catcatgcac gaggctccca tggggccacc ataggccgct
     3661 agcatgggga tcgtgacgac gcttcccgga acgacgctgg cccgcgggga ggtgcttccc
     3721 ggcacgcccc tgatcgacgc ggccaccggc ggggaggtcc gcgtctggga cttccgcggc
     3781 aggacggcgc tggtcctctg cttcctccat ctggcgtgcg aagcctgcga gcggttcgct
     3841 gccgggctcg cggaggcgga gagcgagatt cggctgacgg gcgcatgcgc gttcgccgtg
     3901 ctccgtgagc cggcgccctc ggctctcccg gtcttcgtgg atggagatgg cgacgcgagt
     3961 cggaggttcc tcggaccgga gggcgcgctc cccacgatcc tggtggccga ccggtacgcg
     4021 gcggcgtggg agtcctaccc cgtcctgggg cacgacttcc cgccagcatc ggagatcgtg
     4081 ggggatctct ggcacctcgc cacgatgtgc gaggagtgcg ggatcatggc ctgggagtga
     4141 gtcagtgcat ccccgcagcc ctgaagaggg ccgagaggcc gacgggctcg aggttcctgg
     4201 cccgcaggcc gtccacgatc gatccgatcg aggcggccgt ctgatctccc gtgtgcagca
     4261 ggatgatgct gcccggccgc gccgccgaca acacccgacg tctgatctcc gacacgccgg
     4321 ggctccgcca gtcctggggg tcgacgtccc acatcacgac gcgaggggcc gcggccgcgg
     4381 cggccccctc caccactgac gcgttgcagg cgccgtaggg cgggcggagg tagggggccg
     4441 aggtgcgcct ggcgacctgc caccatgctc ggcggtcggc ccgcacccgc gagctggtga
     4501 acgaggggtc gtgccccgtc aggatcgcgt ggtccagggc gtgcgacccg ggcgtgtgcc
     4561 cgtcccggac cgtgcgtcgg gccaggctgg gattcccccg gacggctcct cccccgcaga
     4621 agaacgccgc ctcgacgttc ttcgcgtgga gggtcctgag gatgctgccc caggctgagg
     4681 tgttcccgca gtcgtcgaac gtcagggcca cccgccgtcc ggtgccctcg aggcgacgat
     4741 agacgcgccc gcccacgttg cgatggaccc gccatgggac ccaccgccgt cgggacacgt
     4801 tcccggcgtc gtcccacagc cggacgccca gccggtagtt gcccgggtag aggcgcttcc
     4861 cgctccgata gcgggccctc cagcgcacgc gcgtcgggcc ggggggcttc tgcaccgcct
     4921 gcttcccgat cgtcccgagg gcatcctcga tgcggagctc cgtgcggatt ctgggggcgc
     4981 gatcccgccc catgaaccgg atcacgagcc accggtgggt cgtgagggga tcgggccgaa
     5041 cgcggaggcg gcggaggcgg ggcggctgcc tgtcgagcac gagccttgtg gcgccggtgg
     5101 cggtcgcgcc ttcctccgtc gcgatgacgg ccacgacttc gtagcggccg tcgggggcac
     5161 gcacctcccc cgaccgtccc cgccacacga cctccacgga ccccggctcg ggaaggagca
     5221 gctccggcca cgcgcgccac gtctcgccgg ccgtgtcctg gatcgacacc gtgaccgagc
     5281 cagcggccga gctcgtcacg gcgatcacgg cctcgtctcg gcggccgtcc ccgttgggcg
     5341 agaaggggtt cgggagcgcg gtgatatcga tctcgatcgg aggtggttcc tcctcgccgg
     5401 tggatgccac agccaagtcg gccgccgaca tgacggcaac gaggagcccg gcgacgagca
     5461 gcgcgcgacg acccacgcct ccacgctaga gcgcgagggc gcgtgcgaag agggccgtag
     5521 gacccgcgta acgggggcag gatgggggcc ggcgcctcgc ggagcgaggc ggcggcccat
     5581 ccctcaggtc agatcccagt tcaggatgcg ctgccgcacc ttggggccca gacccgccag
     5641 agggcgctgg tgagccgctt cttgttgaac ttcctcacgc tggccttcgc gtccgcgtca
     5701 tccccggaca gctcgacgaa gaacagcagg tacgcggtct gcctgggacc gaggcgcaga
     5761 gggaaggccg tgaggatgcc gtcggtaccg gcggtcggcc cgatctggag ggccggacgt
     5821 acggttcgcc ccttgccgaa atgaacgaag gtgacgaccg ggtcgctgaa gggcgtctgg
     5881 tcgctggtga tgcgccaccg gccctccctc ttcctcgcgc gggcttgatc gctggagcat
     5941 agccggcgag taccttggga tcctgaaagc cgggcggcgt tgacggtcag ggggcccggg
     6001 cgtaggcttg aaggctactg acgagtaggt attcggcccg agggagggac cacacatgcg
     6061 cgaagccgtg atcgtcgagg ccgtccgatc gcccgtcggg cgccgaggcg ggaagctctc
     6121 gggctggcac cccgtggacc tcctggctga ggtgctgagc gcggcgctgg agcgctcagg
     6181 catcgacccc ccccgggtcg aggaccacat ctcgggctgc gtctcgcaga cgggggagca
     6241 ggcgatcaac atcgcgcgga acgcttggct ggccgcgggc ctcccggagg aggtgccctc
     6301 ggtcacggtg gatcgtcagt gcggctcctc gcagcaggcg gcccacttcg cggcgcaggg
     6361 cgtgatggcc ggcgcctacg acctcgtgat cgcctcgggg atcgagcaca tgacccgcgt
     6421 ccccatgggc gtgacgatgg agcaggggcc gggattcccg ttcagccagc ggctcctcga
     6481 tcgctacgac ggacgcctcg tgccccaggg tgtctccgcg gagatcatcg ccgagcgctg
     6541 gaacctctcg cggggggagc tcgacgagtt cagcgcccga agccacgagc tcgccgcgaa
     6601 ggccatcaag gacggccgct tccaggacga gatccacccg atcgaggtcg gcaccgaggg
     6661 ggagaccgag ctcttcgagc acgacgaggg ggtccgattc gaggtggacc tggacaagat
     6721 ggccacgctc ccgacgccct tcaaggagga cggcgtggtg acggcgggga actcctcgca
     6781 gatctcggac ggggccgcgg cgatcgtgat cgcctcccgg gagaaggccg aggagctcgg
     6841 cctgaggccg cgggctcggt tccacgcctt ctcgctgtcg ggggtggacc cgatcacgat
     6901 gctgaccggg ccgatcgagg ccacgcggaa ggtgctcgcg tccggggggc tctctctcga
     6961 ggacgtcgac gtgctggaga tcaacgaggc cttcgcctcc gtagtgctgg cgtggaagca
     7021 ggagctcgag atccccgacg actggttcga ggcccgcgtc aactcgaacg gcggagcgat
     7081 cgcgctcggg cacccgctgg gagcctcggg cggccggctg atgaccacga tgctgcacga
     7141 gctcgagcgg cgcgagggtc ggtacgggct ccaggtgatg tgcgagggcg gcggcctggc
     7201 caacgccacc atcatcgagc gcctcgtggt tgactgagct cgtgcggttg gcgatcgcga
     7261 tcctgctcgc ggtggggatc gccggcatcg gggtctggat cctgtcggcg atcggccgca
     7321 accgcgacct cgtcagggag gtacccgtcg aggacgtgga ggagctcgac gtctacttcg
     7381 tctgcggcga gtgcggaacc gagttccggg tcgagaagct cggggagctc caggtgcccc
     7441 gacactgcgg agagacgatg cgggtcgagc agcggccggc gccgctggcc taggactccg
     7501 cgatctcgtc ggccttccgc ttcagatcct tgtagtcgcg ccgggcgcgc cggtcctccg
     7561 ggagcagggc gtcggtcctg gaaaagcccc atccctcgaa ccggacgccc acgcggctcc
     7621 agcgacggcg catccgcgat cccccgcagg cgggacagga gccggggggc tcggtccgcc
     7681 ccatcgggcg caccagctcg agggaggcgc cgcagtcctc gcagcggaat tcgtagacgg
     7741 gcatcaaatc acgcccatgt gattcgttcc acaatgtgga gaagcggtgt ggaaaacgcc
     7801 cgggggggca cgaaagccca ggtcagcgcc actgcgtggc caaagcgaag cccacggcga
     7861 tgaggccgag cccgacccag aggtaggtgt tgttcggcga tccggggagc aggaagccca
     7921 ggtagttcag cacgatcatc aggaccccga cgaacaggag ggtcaggacg aggatcccga
     7981 accaggtggg gctgggcttc ggcttgggcg gcttgggggg ctggtacctg cggcgcttcg
     8041 acttcgactt gggcacccgg ctcctttccg tcgggacggc acctatcata gccgcatgga
     8101 ccggcccggc tcccgcttcg cgatgccact cgccgccggg gtcctgggct tcctcgcggt
     8161 gctggcggcg gctcagcctc aggcggttcc ggggcgcgag tcctcgcgtc tcgagatcgt
     8221 cgacctgatc gagcagcagg acgggcgggt gcggcagctg cgccaggagg tgggggacct
     8281 cgaggctcgg atccgggagg cgagcagcgg cgagacgagc gccgcgatcg gggagctccg
     8341 ggccgaggcg gaggcactgg cggtggccgc cggcgcggcg ccgctccgcg gtccggggat
     8401 cacggtcgtc ctcgacgact cctcggcctc ccgttccgcg accggggacc ccaacgacct
     8461 catcgtccac gagcgggacc tccagaccgt ggtgaacgcg ctctgggccg gcggcgcaga
     8521 ggccgtggcc atcaacgggg agcggctgtc gagcctctca gccgtccgct gcgcgggcaa
     8581 caccctgctg ctgcacggtg tcgtgcacag cccgccctac gagatccggg cgatcggcga
     8641 tcccgaggcc ttggagcgat ccctggagga cagcccgggg atgcggcgcc tgcacgaggc
     8701 ggtgcgcgcc ttcggtctcg ccctcgagat cgagcgaggc tccctggtcg tgcccgtggg
     8761 gcgcggggtg agggagctgg cgacggcggc gccggcggca tgacggtgga agccccgccc
     8821 aggtccggcc gccggcgtcc tcgtggcgct cttcgtcgtc tacgagctct tcgtcaccga
     8881 cgtcatcacg gggcggcacc aggacgcgct ccgccaggac ctcggacgac gcctccaggt
     8941 ggcgggcccg aaccgggatg tccgtccggt tcccggcaga ggggtggcga tccttcggat
     9001 cccccggatc gacctcgacg ccgtggtggt ggagggggtc gggctcgagg acctcaagaa
     9061 gggccccggg cactaccctc ggacgccgat gccgggtgat ggcggcaacg tggggatcgc
     9121 cggacaccgg accacctacg gcaagccgtt ctggtcgctg aacgagctga ccccgggcga
     9181 cgagatcttc ctccggacga gcgggggcga gttcgcgtac gaggtgctct ggacgcgggt
     9241 cgtccttccc agccagtcgg aggtcctgga tcggacccgt gggccgtcgc tgacgctgac
     9301 cacgtgcgag ccgcggttct cggctgcgag gcggctgatc gtccgggccg tgcaagtcga
     9361 cccgccgccg gatccggagg cgcccggatg atccgcttcc tccgccgacg gagccccggc
     9421 aaccccctgc tggcggtcct ctactgggtc gtcctcgccg ccaccgtcgt gatcctcgtg
     9481 ttcgcgctct tctacctcct cgacgtcagc cgcttcgtcc ccggcggcgg gatgttctag
     9541 ccttcgcccg tgaccgagga gatcgcggcc accgacgcgt acgcacgaga gacccgggcc
     9601 accgtcgtgg gatccgggga cgacggggtc gtcctcgatc ggaccgtctt ctatgcgcgg
     9661 ggaggcggac agcccggcga caccggcgcc ctgcgctggg acgggggaga ggtccgggtg
     9721 gtcgacacgg tgaggcgcga gggccgggtg ctgcacgagg tcgagggcga cgcgccggcg
     9781 ccggggacgg ccgtcactgc cacgatcgac tgggagcgcc gtcaccgtct gatgcgcatc
     9841 cacacggccc tgcacgcgct gtcgggcctg atctggcgcg actacgacgc caaggtgacc
     9901 gggggcaaca tggatgaggg ggccgcccgc atggacttcg agctcggcga gatgagcgcg
     9961 gagttcggcc gcgaggtcga ggagaagctg aacgcggccc tggccgagga tcgtccggtc
    10021 cacgtccggt tcctgcctcg ggccgaggcc ctccaggacc ccgacctgat ccggacgaag
    10081 gtgaacctca tccccgactc ggtggacccg atccgcgtga tcgacatcga ggggatcgac
    10141 cgacaggccg acggcggcac ccacgtccga tcgaccggcg aggtgggccg ggtccgcgtg
    10201 acgaagaccg agagcaaggg caaggccttc aagcggatcc gcatcgccct cgaccagggc
    10261 taggggacca ggcgcaggac ggaggccgtg gcccgctcga ccgcgtcgcg cgtgaacggc
    10321 atcggatggt gctcccccga cgcccacagc gggagctggt cgcggtagtg ggggctctcc
    10381 gggttccccg actgcccgag ggtgagcaca ccgaccgacg cgtcgaggtc ccacaggtcg
    10441 acgatctgcc gccagctcgg gaccaccacc gcctcgtagg gagccccggg ttcgaagtgg
    10501 gcctgagcca cggtctgctc gtcgcctccc agctcgacct cgccgacggt gaagagctcg
    10561 ccgagcccgg gcgccgcggc caggcggccc gcgaaccgag ccctgtgcac ggccccccag
    10621 cgccagtccg tcatgggctc gcccagccga tcggtgagct cggtcagcgc ttcgtccagc
    10681 gcggcgcgga ccacgtcgtc gcgggcggcg gccccatcct cgccgaacca gcgcgccgtg
    10741 ggataggcga ggagctcggg gagggcccgg tagtggaagc cgttcgtcca ctggcggcga
    10801 gcgaagtagt gctcgaagag ctccctgccc agcctcggcc ggaggatccg ggcggcgagg
    10861 tgagtgcacc atgactcgta gatcgcagcg gcggccgagt cgcgcccgag atcctggtcc
    10921 cactcgatca tcagcccgag cgcctccgtc tgccgctcgt cccgggcctc gatcgccagg
    10981 aggtgccgca ggatctcggg agcgagcagc gaccgcgtgt cggcctggat cgcagcgaag
    11041 ctctctcggt cgtggcgggg acgagccgtc agcagctgcg cgatccggcg ggcgcggtag
    11101 ggcgggagga agtcgacgcc gagggaggac tggccgtcgc cgccgggggg gttgttcgcc
    11161 gtgaccacga acccggcttc cgggttgaag gccctgggca gctcgtcgaa cgggatccaa
    11221 ccgtcccact cgtgctcgtc ggtccagccg ggcacgggga ggcttccgtc gcccgcccgc
    11281 cgcgccgggt accgaccggt gagctggtag ccgatgttcc cctccacgtc tgcgtagacc
    11341 atgttctggc ccgggcagtg ccagcgcgcg acggccgctc ggaactcctc ccacgagcgt
    11401 gcggtgtcga gccggtggat cgtggagggc tcgatcgcgc cctccgctcc cacccaccgg
    11461 agggcgtacg gtcgtcggat ccctccctgg atcacccggg gggaggcgat ccccaccatg
    11521 tacgagccca cgaggggacc gtggcgcgtc tcgcgcacct ccaggacctc cggctcggcc
    11581 cgcccccgca ccccgatctc ctcccggacc acggtcacgg gctcccagtc gtcgaggtgc
    11641 ttcgccgacg ccccgtcctc ggagagccgc tcgagataca ggtcctggac gtccccctcg
    11701 gagttcgtga acccccacgc gatccggtcg ttgtgaccga tcacgatgcc ggtcgtgaag
    11761 gggaacgtca cgccggccac gtcgacgccc ggcccgacca ggtgcgcctc gaaccaggcg
    11821 gacggggcct ggaccacgag atgggggtcg ttcgccagca ggggggcgcc ggtcgcggat
    11881 cgcgagctcg ccacgaccca cgcgttcgag ccctcaccgg agggggcgag gatcgcgttc
    11941 cgcagcatcg ccagccgcgg atcggtggcc tccttggccg cgcggacggc ctcgggctcg
    12001 gggagcgcgt ccgggaagag cgcgcgcatc gcctcgaccc cgaagcgctc ggcgatctcc
    12061 gcccgcagga ggtcgttgtc ccaggcccgg gagagcgacc aggcgacgta cacggccgcg
    12121 ccggcgccgg cgagcgcggc ctcgtcctgg tcggggagcc aggggtcgac gccgagcacg
    12181 acgtactcga cgggcttggc cggcatcgcc tcgagccagg cccgggcgcc ggcggcgccg
    12241 gccgcggcca tctcgagcga gaggtcgtcc caggccgccg cgtgctccgc cgcggcccgg
    12301 ttccagccga gggtcctcac gaaccggtcc atcgagagcg tgagctcccc gaagagctcc
    12361 gacaggcgcc ccagcccgag ccggtaggcc agctcgatct ggaagagccg ctcggaggcc
    12421 atcacgtacg actgcgcggt ccagaggtcg gtcaggtggt cggcgtagat gtgaggcacg
    12481 ccccagcggt cccgacggat ctcgaccgca gccccgaggc ccgggaggcg gacctctccc
    12541 tcgacgggcg ggagggcggc gcgggcggcc ccccggaggg cctcgatcgg atcgtccacg
    12601 gggtcaccct agggccgccc gcgccgcccc ggcaagaccc cgctggcccg tccgcagcgc
    12661 cctgtggaat gatgtgggtc tccgcgatga ggaggtgcgc caccgatggc gaacgacagg
    12721 acggagcgcc ctcgcccctg gctcgacctg tacgcgcagg gcgttcccca cacgcttcag
    12781 ccctaccccg agatgtcggc cttcgagatg ctggagcgca ccgtccagcg cttcccggac
    12841 agtccggccg tggccttccc cgtcgccccg atggcgagga cgctgaccta tcgccagctg
    12901 ctggacgagg tcgagcagat gtcgcgcgcc ctcgcctcgc tcggcgtgaa gcgcggcgat
    12961 cgggtgggcc tcctcctccc gaactccccg cacttcgtgg tggcccactt cgcgatccag
    13021 cgcctggggg cggtcaccgt ggggaacaac cccctctaca cgaagcggga gctcgtgcac
    13081 cagctgaacg acgcgggggt cgagatcctg atcgccctcg acctgctgta tccgctggtg
    13141 gaggcggccc agacggaggt cgccttccgg aaggtgatcg tggggaaggt cggggactac
    13201 ctgggcttcc ccatcaacaa gctggctccc ctgaagcaga ggcgagaggc gaagcatgag
    13261 gggcgtcgct ggccgccggt ccctcaggac gccgaagtga cgtggtgggc cgacctgatg
    13321 aagggcagct atcccgacgt gccgccggcc caggtcgacc cgaaggagga cgtggccgcg
    13381 ctcgtctaca cgggcggcac gacggggctg tccaagggcg cgatgctcac ccacctcaac
    13441 atcgtggcca acgccgccca gacggcggcg tggttcacgg ggatcgagga ggggcacgag
    13501 gggatcgtgg ccgtcctccc cttcttccac tcctatgggc tgggtgcgat catgggcgtc
    13561 ggcgtctaca aggggctgaa gctcgtgctc ctgccgcgct tcgagctgga cctggtgctg
    13621 aaggcggtgg agaaggagcg ccccaccctg ttccccggtg ttcctcggat cttcatcgcg
    13681 atcaacgagt ccccggagac ggcgaagcac gacatctcct cgatccgctc ctgtttctcg
    13741 ggcgcggcgc cgctccctgt ggcggtcgcc gagaggttcg agcagatcac ggggggccgt
    13801 ctcgtggagg gctacgggct cacggagacc tcaccggtga ctcacatcaa cccgctggac
    13861 gggtcgcacc gcttcgggac gataggcctt cccatccccg acaccgactg caagctcgtg
    13921 gacatcgacg agcccctgaa ggaggtcggc ggggatcagg agggggagct ctgcgtcgca
    13981 ggacctcaga tcatgaaggc ctactggaac cggccggagg agaccgagga catgatccgc
    14041 gtccaccccg acgggatccg gtggcttcac acgggggaca tcgcgaagat ggacccggac
    14101 ggcttcttcc ggatcgtgga ccggaagaag gacatcatca tcgtcagcgg gttcaacgtg
    14161 taccccaccg acgtggagca ggtcctctac cgccacccca agatcgagaa ggtctcggtc
    14221 gtgggcgtcc ccgacgagac gaccggcgag gccgtgaagg ccttcgtgat cctgaaggcc
    14281 ggtcaggagg ccaccgagga cgagatcaag gcctgggccc gcgaccccga gcacggcctc
    14341 acggggtacc gggcgcccag gcacgtggag ttccgggacg agctccccga gacgatggtg
    14401 ggcaaggtgc tgcgaaggaa gctccaggag gaggagaagg agaaggcctc ggctcaggag
    14461 tcgtagcgac gggaccgcag ccggtcccag agcaccatcc acgtcccgat cgacgtcagc
    14521 agcgaggcga gggcgagcgt ccccagggcc aggaacagcc agtcctgctt cgtcacgccg
    14581 aaccccgccg cgaggccgcg cgccgctcgg cctccagccg ctccaggtcg acctcggaga
    14641 ggtccacgtg ccgcatggaa ccggcgatcg cccggtggcc ggcctcgtcc cggatcagcc
    14701 ggtgcatctc gtgggccacc cgccggtacg cggggtgccc ctgtggtgag gagcggagct
    14761 cgatcaggtg catcgcctcg cgtgcgttca tctgcatcac gaagcggatc cggtaggcca
    14821 tcgccaccgc gtactgggcc acccctccga ggcccgcggc gcggagctcg ccctcgagcg
    14881 cggccgatgc ctccatcacc cggtcccaat cctcccgcgc tccggcctcc tcgacgtcgt
    14941 tcgggcgctc gaagccgtgg gcggttgtca gctcctgcca ctcaatcgtc agcatccggt
    15001 ggcgctggag gtcgcggaac gccccgtagt cgcagagcac gtcgaaccgg tagccggccc
    15061 gctcgaaggc ccggccgggc ttgtgccggc ggttcgggcg gtcgccgacg taggccgtga
    15121 ggacgcggtc gcgctgctcc gtggacatgg cgcgggcccg ctcgagcagc acgtcgtcgg
    15181 ggagaccggt cgctgcgtag agcgcggcgg ccaccacctt cacctcgccc tcggggtcgg
    15241 agtcggtcag cgcgacctcc ggtcgggggg gcgcctggag gtcgccgacc agctcctcgg
    15301 ccaccttggc cgtctcctcc cgggtccggg ccaggtagtc ggaccaggcc ccgccccggt
    15361 ctgggaggtc cacgcgccgg aggaatgccg ggatcacctt ccgcagctcg gcgagcatca
    15421 ggtcggcgta ggcgcggacc tcggcgtagg ggtgcccgcg catccgcagc aggagttgtt
    15481 cgtaggcctg gcctgtggcg tagatgccca cgttggtgcg agtggccgcg gggagcaggc
    15541 cgcgcagggt gtcgcaggcc ttggcccgga tcgtggagcg gtagacgaag tccgggtcgc
    15601 cggcgtcctg ggggtgccgg gcccggtaca gctcctgcat cggctccagc caccgcgcgt
    15661 aggtgtcgaa cgcgtggtcc agcacctccg agtagcgggc cgagagcccc gcggcctcga
    15721 cctcgggcgg gatcgcgtag cggtagcggc cgccgggccg gtcgtcgtag ggcacgtacc
    15781 gggtggactg ctccaggtag gccgcgagac gcccccactc cagcaccttg gccaggatct
    15841 gcgaggactg ctcgcacgcc aggtgcacgc ccccgagctg ggccaccgag tcgtccccgt
    15901 actcgaagaa gacgttctcg tagaggcgct cggcccgggc cacgccggcc tccccggccc
    15961 cggctccgcc ctcggcctcc tccaggaact cgtcgagcag cagccgccgg acggacttcg
    16021 acgaccgaga gtagcgggcg aagagcgcgc ccttcgtcac ctcggggagg ttgaccaggg
    16081 cgaacacggg cccttccagc gaggtgacga agggcgcgag gcgctcccgc tcctcgtcgg
    16141 tgaaggtctc ttcgacccag ggaggctcag gcgccatcgg tcggggagtc cgtggggctg
    16201 ggctcgggcg aggggcccag cgagatccac acccgaaccg tggagcccgg cgcgacctcg
    16261 gcgccggcct ccgggtcctg gcgggcgacg gtgccggacg ggcagtccga gaactcctcg
    16321 gtctcctcct ggtagacgag gtcccggccc tcgatctcgc tccgggcgtc ctcgggcgag
    16381 tcgcagacca cgctgggaac ggccaccgtc tcgggaccgg tggagatcac gatgttgacg
    16441 gccgagccct cctcgacctc cgtctcgggc tgggggtcct gggagatgac gatgcccggg
    16501 tcctcctcgc tcggctcctg tcccacgaag ccgagggtga gcccggcatc ctcgatcgcc
    16561 tgctgggcct cctcctggga gaggccgacc acgctgggga cctccaccat gcgggtcccg
    16621 cggctgacga agatccggac cagggagccc tcgtccacct cggtgccggc ctccgggtcc
    16681 tgccggaaga cttggtcggc ggggacgtcg tcccgagccc tccgcacgac ctcgacctcg
    16741 aggccggcct cctccagggt ctggacggcc gcgacccggt tgaagcccac cacgtcgggc
    16801 accgtcacgg gctcggggcc cgtgagcagc gctcggacta ggaagaacga ggccagcccg
    16861 aggatcgcca ggatcaggag ggtccagaag acgatccacc cgacgcgccg gcccctctcc
    16921 tcctcctcct ccatctcctc ctcgagggga ccggtgagga cctgggtgtg accgccgcgg
    16981 tcgatcacct gggtcgtgtc gccggggaga gggacggccg cggccaccgg catcccctcg
    17041 gaggctcgcc ggagatcgtc gatcagctcc tgcgcgctct ggtagcgatc gccgggcgcc
    17101 ttggccatcg acgtcatcac gatcgcgtcg aggctctccc ggacgtcggg gttgaggcgg
    17161 gagggcgggg tcgggtcctc ggtgacgtgg cggtaggcga tcgagacggg actgtcgccg
    17221 gtgaacggct ggcgccccgt gagcatctcg tacagcacga cgccgagcga gtagatgtcg
    17281 gagcggtggt ccacggtctc gccctgggcc tgctcggggg agaggtacgc ggccgtcccc
    17341 aggaccgccg ccgtctgcgt caccgtgtcg gaggtcgtgg cccgggcgat gccgaagtcc
    17401 atcaccttca cctggccctc gcgggtgatc atgatgttgc ccggcttgat gtcccggtgg
    17461 acgagcccct tggcgtgggc cgccgagagg gccttggcca cggcctcccc gatctcgatg
    17521 ctgcgctcgg gggtgaggcg gctctcctcg cggagggtgt cctggagtgt cctcccctcc
    17581 acgtactcca tgacgatgta gtggacgttt ccggtggagc ccgtgtcgaa gaccgagacg
    17641 atgttggggt ggttgagggc ggcggccgcc tgcgcctcgc ggcggaaccg ggccacgaac
    17701 tgctggtcct cggcgtactg gggcgacagc accttgacgg ccacggtgcg ccccaggacc
    17761 cggtccgtcc ccaggaacac cttggccatg ccgccttcgc cgaggacgcg ctcgatctcg
    17821 tagcggttgc cgaggacccg ctgcttcgcc atctggtgag actctatcgg gtcgagggcg
    17881 cggcgggggt catccgccta ggtgtcgtcg tcctgcccgg gagggccctc gccgtcgggc
    17941 ccggggggcc cctccccgtc cgggcccccg ccgcccgagc cgtcttcgtc ctcgtcgtcg
    18001 tcctcctcct cgtgctcgcg cggcgggtag gcttcgatcg cggcctggag ggcgaggatc
    18061 cggtgctcga tctcggaggc tcgctcgggc gtgacctccc cctgctcggc tccgtccacg
    18121 agggcgttga tggcgtcccc ggccttgtcc aaggccttct gccggtcccc ctcggcgtag
    18181 gcctccatcg cgtcctcggc ccgttcgacg atctcccgcg ccagcttctc ctcgatctcc
    18241 ccggccgtga gcccgtcctg cacggctccc acgagggccg tgaacgcctc ctgtggccgg
    18301 agctgctcgg cctcctcccg cggcgactcg gccggggacg gccctgcggc cgctgcgcca
    18361 cggtcgcggt cgcccctgga cgcggcggtc gggtcgtccc gaccgaaggc cagcacgagg
    18421 cccccgatca gcgcggcgag gaggagggct ccgaggagcg ccgggagcac ccacctccga
    18481 cgaaggggcg cgggcggggc cggcactgcc tcggtctcga gcgcttcctc ctccagcacc
    18541 atcgtgtccc cggccccgcc cgcgcccccc cggatcgccg cagccatcga gcgcgcgtcg
    18601 gggaaccggt cgtccgggtc cttctccagc gcctgcatca cgacggcggc cagcgacggc
    18661 gggaccgacg acggcagcgg cggcggatcc tcgtggatgt gcttggtggc gaccgagacg
    18721 gggctccctc cacggaacgg cggcgccccg gtcagcatct cgtagaggac gcatcccatg
    18781 gcgtagaggt cgcaccgggc gtcgaccggg cggcccctgg cctgctccgg ggagaggtac
    18841 gcggcggtgc cgagcacctt cgagccaacc gtgatcgtct cggaggtggc cgccctggcg
    18901 atcccgaagt ccgcgacctt gggctcgccc tcgctcgtca ggagcacgtt ccccggcttg
    18961 acgtctcgat gcaccagccc ccggtcgtgg gcggccgcca gggcctccag gatgtcggcg
    19021 gccaggagcg ccacgcgttc gggatcgagg ggcgcctcgc gcgccagcag gtcggccagc
    19081 gtctccccat cgatcaactc catcacgatg aaccggcgac cgtcgtcctc tcccgtgtcg
    19141 tagacggcca ccgtcccggg gtgggtcagc cccgccgccg cgcgcgcctc ccggcggaac
    19201 cgctccacga actcggggtc gcgtcccagg gaccggtcca ggaccttgac ggccacggtc
    19261 cggtcgagga cgcggtcggt gccccggtac acgctggcca tgccgcccgc gcccagcggc
    19321 tcctcgagct cgtagcgatc cgccaggacc gtcgtcatga gcctccctcc agcgcggcct
    19381 ccatgaccgc cttcgcgatc ggggcggcga tcgccccgcc ggtcgcctcg ctgctgaggt
    19441 ttcccctgcc gaggacgacc acggccacgc tgggctgctc ggctggggcg aacgcgatga
    19501 accaggcgtg aggcggctga ccctcggccg tctgggccgt cccggtcttg cccgcgacgc
    19561 tcacgcccgg gatctgcgcc ccggtcgcgg tgcccgagga gaccacggag accatcatct
    19621 gggtcagcgc ccgcgcgttc ccgcccgaga gcggcctacc gtagacctcc gggtcgaacc
    19681 gctcaatcac cctcccggag gggtctcgga tctccacgac gaggcgtggg cgcatcatca
    19741 cgccgccgtt cccgatcgac cccgccacca gcgccatgtg gagcgggttc gtcctgacgt
    19801 cctgctggcc gatcgccgag aaggccagcc ccgggaggtc ctggtcgaag gccgacggat
    19861 cggggacctg gccctcggcg aaggggatgt cgaacgggac gtcctcggag aacccgaagc
    19921 gcctcgcctg ctccatgagc gcctcggcgc ccacctccaa gcccacctgc ccgaaggtca
    19981 cgttgcacga gacctggagc gcctgcgcca gcgtgatctg cgaggctccg cccaggcagt
    20041 gggatccgcc gaagttgtcg aggttgttcg tggtctgggg caggtccagg ctcggcggat
    20101 tgtcccacgt ggattcggga ccgaggccgt tctccagcgc cgcggccgcg gtcacgacct
    20161 tgaaggtgga gccgggcggg aagacctcgt cgttcgcgtt cgagagcatc ggcttgtcgg
    20221 ggtcatcgtt gagctggttc caggcccgtc ggacctcccg gccgtcgtgc gacgagaggc
    20281 ggttggggtc gaacgtggga agccccacga gggccagcac ctcgccggtc cg
//
LOCUS       DASUTW010000156         4917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_956760, whole genome shotgun sequence.
ACCESSION   DASUTW010000156 DASUTW010000000
VERSION     DASUTW010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956760"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VF058_05255"
     CDS             complement(<1..357)
                     /locus_tag="VF058_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014910791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6207750.1"
                     /translation="MSLLSVRDLHVTYSTEGGPVPAVRGVDLELSRSEILGLAGESGC
                     GKSTVAHALLRLLPKGTEVTGQVLLEGEDLLAMKPGRLRAVRWAEASIVFQGAMHSMN
                     PVQRVGDQIAEAMVLHG"
     gene            complement(354..1295)
                     /locus_tag="VF058_05260"
     CDS             complement(354..1295)
                     /locus_tag="VF058_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009068973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6207751.1"
                     /translation="MSDGTIAPPAEPGAPPAPPSPRAIAWSRRRRSAARVWRTFRKNR
                     MGMTGLVILVFFVLVALFAPLLADPAGLRATEATGGVLEGPSLEYPLGTDILGRSVLT
                     LTIHGSRISLLVGFAATLLSMLIGSVLGIAAGFYGRWRDAVLMRLTDWFLVIPFLPLA
                     IVLASLIGPSLPTIIFVIGVTSWPGTARLVRAQTLSVKERPYVERARALGAGGWHLVT
                     RHILPNVFPIIFANTTLTVAIAILSETTLSFLGLGDPLRVSWGSILEFAFSAGASTAG
                     AWWWLLPPGLAIVLVVLAFTMVGYALDEILNPRLRSR"
     gene            complement(1292..2263)
                     /locus_tag="VF058_05265"
     CDS             complement(1292..2263)
                     /locus_tag="VF058_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6207752.1"
                     /translation="MGTRRYLLGKVAQATLTVLFVLIFNFFLFRVIPGDPAQILARAK
                     HLPKEAVVQLERDFGLHEPIPKQFALYVEDTVTGNLGISYKFRRPVAGVIAERIWPTV
                     LLVGISTLAATAIGLLIGIYGAWRRGSKLDLGSLGTSLVFYAMPEFWLGILLLMGFAA
                     GLGWFPTGGIVTPGAGYTGVAAATDVLNHLFLPAVTLTLALIGEYALIMRSSLLDVMG
                     EDYLVTARAKGLREALVLRRHAVRNALLPTVTLVALNLGFVVSGAITVELVFSWPGLG
                     LLTAEAADVPDFPLLQGLFLLFSVTVILANLVADLIYGYLDPRVRAA"
     gene            complement(2292..4121)
                     /locus_tag="VF058_05270"
     CDS             complement(2292..4121)
                     /locus_tag="VF058_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6207753.1"
                     /translation="MTRRRGRGLAALTATALLIGLYAPGAGAQTPAPTAAEDEKITFV
                     YGDDNDIDSMNPWVGVEAPAYLMYALNYDLLVNFSIEDLSPSPGLAESWEVSDDGLTW
                     TFDIREGVTWHDGEPFTAHDVAFTFNRILEERQGCCIDYIRQAETVEATDDTTLVITT
                     RRPTLQFLTAYVYILPEHIWSDISEEEAKTYENYPNPVGTGAFRVVEWEEGQFFRMEA
                     NPDYWAGAPEVDEVIYRIYQNEDALAQALLAGEVDFADTLGAGIYDSLEGQPDIGTNE
                     ANIPSFEEIGFNAGADDTYGDEADGHPALKDVTVRRAIAHAIDKETIVDRVVRGHGTV
                     GTTIVPEVTPFYHYEPTEEELIPFDLEEANRLLDEAGYEDTDDDGVREMPGGGQPLHF
                     RYFVRNEKQTTVTASEFITDWLEQIGISTEVRGLSDTRLTNLIYDGDFDMFHWGWFPD
                     ADPDFILSVMTCGQRPPDGIWSDSFYCNEEYDRLYEEQQTTLDLDERAEIVKEMQRIV
                     YEDAPYIVLFYDRSLQAYREDRWTGFRQQPADGGDLLAAYGPFSFISIRPKTTEEGGT
                     GQTGATSGTGIPTGVWLGLIGAVLAVAIVWMLLRRRGAEEERE"
     gene            complement(4118..>4917)
                     /locus_tag="VF058_05275"
     CDS             complement(4118..>4917)
                     /locus_tag="VF058_05275"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX6207754.1"
                     /translation="IDRARDVEAMFADPEVDAVQVFGGGYGSSQVVPLLDTDVIAQNP
                     KPFVGYSDITALHVAIRQATGLVTFYGPGLAGIAYEASEDRKFTQDRALRALTDPTPL
                     GELPARPDDAYVGAIGAGVARAPLVGGNLWLMRETLGTPWEIETAGCVLFFEDVYTPP
                     WHVDGMITHLANVGKLRQVVGVVVGEMYRSEEWRHPEPWLRSRSMEDVLEYHLEPLGV
                     PVLTNLPLGHGKHLATIPLGVEVTVDADERRLVVEESALQEAEGGER"
BASE COUNT          758 a         1741 c         1695 g          723 t
ORIGIN      
        1 cccgtgcagg accatcgcct ccgcgatctg gtcgcccacc cgctggaccg ggttcatcga
       61 gtgcatcgcg ccctggaaga cgatcgaggc ctcggcccac cgcaccgcgc gcaggcgccc
      121 cggcttcatc gccagcaggt cctcgccctc cagcaggacc tggccggtga cctcggtccc
      181 cttgggcagc agccgcagca gcgcgtgcgc cacggtcgac ttcccgcagc ccgactcacc
      241 ggccagcccc aggatctcgc tccgcgagag ctccaggtcc acgccccgca cggccggcac
      301 cggcccgccc tcggtcgagt acgtcacgtg cagatcccga accgacagca ggctcatcgg
      361 gacctcagcc tcgggttgag gatctcgtcg agcgcgtacc cgaccatggt gaacgccagc
      421 accaccagga cgatcgccag gcccggcggc agcaaccacc accacgcacc ggccgtggag
      481 gcgcccgcgc tgaacgcgaa ctccaggatg ctgccccacg acacccgcag cggatcgccc
      541 agccccagga acgagagcgt ggtctccgag aggatcgcga tcgccaccgt cagcgtcgtg
      601 ttggcgaaga tgatggggaa gacgttgggc aggatgtgcc gggtcacgag gtgccatcct
      661 ccggcgccca gcgcccgggc tcgctcgacg tacggccgtt ccttgacgga cagggtctgc
      721 gcccggacga gccgcgcggt gcccggccac gacgtcaccc cgatcacgaa gatgatcgtg
      781 ggcagcgagg gcccgatcag cgaggccagc acgatcgcca gcgggaggaa cgggatcacg
      841 aggaaccagt cagtgaggcg catcagcacg gcgtcgcgcc agcgcccgta gaatcccgcc
      901 gcgatcccga gcaccgaccc gatcagcatc gagagcaggg tcgcggcgaa ccccacgagc
      961 agggagatcc gcgagccgtg gatcgtcagc gtgagcaccg accggcccag gatgtcggtg
     1021 ccgagcgggt actcgaggct cgggccctcg agcacgccgc cggtggcctc ggtggcgcgc
     1081 aggcccgcgg ggtcggccag taggggagcg aacagcgcca cgagcacgaa gaagacgagg
     1141 atcaccagcc cggtcatccc catccggttc ttccggaacg tgcgccagac ccgtgcggcg
     1201 ctccgccggc gccgggacca ggcgatggcg cgtggcgacg ggggcgccgg cggagcgccc
     1261 ggctcggccg gaggcgcgat cgtcccgtcg ctcatgccgc cctcaccctc gggtcgaggt
     1321 agccgtagat gaggtcggcc acgaggttcg ccaagatcac cgtgacgctg aacagcagaa
     1381 acagcccctg gaggagcggg aagtcgggca cgtcggcggc ctccgccgtg agcaggccga
     1441 gcccgggcca tgagaacacc agctccaccg tgatcgcgcc cgagaccacg aaccccaggt
     1501 tcagcgcgac cagcgtcacc gtggggagga gggcattgcg gaccgcatgt cgccgcagga
     1561 ccagcgcctc ccgcaggccc ttggcccgcg ccgtcacgag gtagtcctct cccatcacgt
     1621 cgagcaggga cgaccgcatg atcagcgcgt actcgccgat cagagccagc gtcagcgtca
     1681 ccgccgggag gaacagatgg ttgaggacgt cggtggccgc cgccaccccc gtgtacccgg
     1741 ccccaggcgt cacgatgcct ccggtgggga accaccctag ccccgccgcg aagcccatca
     1801 gcaggaggat ccccagccag aactcgggca tcgcgtagaa caccagagac gtccccagcg
     1861 accccaggtc cagcttgctc ccgcgtcgcc aggccccgta gatcccgatc aggaggccga
     1921 tcgcggtggc cgccagcgtc gagatcccca ccagcaggac cgtgggccag atccgctcgg
     1981 cgatcacccc cgccaccggc ctccggaact tgtacgagat cccgaggttg cccgtgacgg
     2041 tgtcctcgac gtacagcgcg aactgcttgg ggatcggctc gtgcagcccg aagtcgcgct
     2101 cgagctgcac gaccgcctct ttggggaggt gcttggcccg cgccaggatc tgcgcggggt
     2161 cgcccgggat cacgcggaac aggaagaagt tgaagataag gacgaacagc acggtgaggg
     2221 tcgcctgggc gaccttcccg agcaggtagc gcctcgtccc catggggcct cccgtcccgc
     2281 gccccggtac gctactcgcg ctcctcctcg gcgccccgcc ggcgcagcag catccagacg
     2341 atggccacgg ccagcacggc cccgatcaag ccgagccaga cgccggtggg gatgcccgtg
     2401 cccgaggtgg ccccggtctg tccggtgccc ccctcctccg tggtcttggg ccggatgctg
     2461 atgaagctga acggcccgta cgcggccagg aggtccccgc cgtccgcagg ctgctgccgg
     2521 aagccggtcc atcggtcctc gcggtaggcc tggaggctcc ggtcgtagaa cagcacgatg
     2581 tacggcgcgt cctcgtagac gatccgctgc atctccttga cgatctcggc ccgctcgtcg
     2641 aggtcgagcg tcgtctgctg ctcctcgtac aggcggtcgt actcctcgtt gcagtagaag
     2701 gagtcgctcc agatgccgtc cggcggtcgc tggccgcacg tcatcaccga gaggatgaag
     2761 tcggggtcgg cgtccgggaa ccacccccag tggaacatgt cgaagtcgcc gtcgtagatc
     2821 aggttggtga ggcgcgtgtc gctgaggccg cggacctcgg tcgagatccc gatctgctcg
     2881 agccagtcgg tgatgaactc gctcgcggtc accgtggtct gcttctcgtt ccgcacgaag
     2941 tagcggaagt ggagaggctg cccgccgccg ggcatctcgc ggacgccgtc gtcgtcggtg
     3001 tcctcgtacc ccgcctcgtc cagcaggcgg ttggcctcct cgaggtcgaa ggggatcagc
     3061 tcctcctcgg tcggctcgta gtggtagaag ggcgtcacct ccgggacgat cgttgtgccg
     3121 accgtgccgt ggccccggac cacccggtcg acgatcgtct ccttgtcgat ggcgtgggcg
     3181 atggcccggc gtacggtaac gtccttgagg gcggggtgac cgtccgcctc gtcgccgtag
     3241 gtatcgtcgg cccccgcgtt gaacccgatc tcctcgaagc tggggatgtt ggcctcgttc
     3301 gtcccgatgt cgggctggcc ctcgagcgag tcgtagatgc cggccccgag cgtgtcggcg
     3361 aagtccacct cgcccgccag cagggcctgg gccagggcgt cctcgttctg gtagatgcgg
     3421 tagatcacct cgtcgacctc cggggcgccc gcccagtagt cggggttggc ctccatccgg
     3481 aagaactgac cctcctccca ctcgaccacg cggaaggcgc cggtccccac ggggttggga
     3541 tagttctcgt aggtcttggc ttcctcctcg gagatgtcgc tccagatgtg ctcggggagg
     3601 atgtagacgt acgcggtgag gaactgcagg gtcggtcgcc gcgtggtgat cacgagggtg
     3661 gtgtcgtcgg tggcctcgac ggtctccgcc tgccggatgt agtcgatgca gcagccctgc
     3721 cgctcctcga ggatccggtt gaaggtgaag gccacgtcgt gcgcggtgaa gggctcgccg
     3781 tcgtgccagg tcaccccctc gcggatgtcg aaggtccagg tgaggccgtc gtcactcacc
     3841 tcccacgact cggccaggcc gggcgagggc gagaggtcct cgatcgagaa gttcaccagc
     3901 aggtcgtagt tgagcgcgta catcaggtag gcgggcgcct ccacgcccac ccacgggttc
     3961 atcgagtcga tgtcgttgtc gtcgccgtag acgaaggtga tcttctcgtc ctcggccgcc
     4021 gtcggcgccg gcgtctgcgc cccggccccg ggtgcgtaca gcccgatcag cagggccgtc
     4081 gcggtcagcg cggcaagccc tcgtcccctc ctgcgcgtca tcgttccccc ccttccgcct
     4141 cctggagggc cgactcctcc acgaccaggc gtcgctcgtc cgcgtccacg gtcacctcga
     4201 cccccagcgg gatcgtggcc aggtgcttcc cgtgccccag gggcaggttg gtcagcaccg
     4261 ggaccccgag gggctcgagg tggtactcca gcacgtcctc catcgagcgg ctccggagcc
     4321 agggctccgg atgccgccac tcctcgctgc ggtacatctc gccgacgacc acgcccacca
     4381 cctggcggag cttccccacg ttggcgaggt gggtgatcat gccgtccacg tgccacggcg
     4441 gcgtgtagac gtcctcgaag aagaggacgc agccggccgt ctcgatctcc cagggggtgc
     4501 ccagcgtctc ccgcatcagc cagaggttgc cgccgacgag cggggcgcgg gcgacgccgg
     4561 cgccaatcgc gccgacgtag gcgtcgtcgg ggcgggccgg gagctctccc agcggggtgg
     4621 ggtcggtgag ggctcggagc gctcgatcct gggtgaactt ccggtcttcg gaggcctcgt
     4681 aggcgatccc ggccaggccc ggcccgtaga aggtgaccag cccggtggcc tggcggatcg
     4741 cgacgtggag ggcggtgatg tccgagtagc cgacgaaggg cttggggttc tgggcgatca
     4801 cgtcggtgtc gaggaggggg acgacctggg aggagccgta gccgccgccg aagacctgga
     4861 cggcgtccac ctcggggtcg gcgaacatcg cctccacgtc gcgggcccgg tcgatcg
//
LOCUS       DASUTW010000157         4804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1214755, whole genome shotgun sequence.
ACCESSION   DASUTW010000157 DASUTW010000000
VERSION     DASUTW010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214755"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VF058_05280"
     CDS             <1..996
                     /locus_tag="VF058_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX6207755.1"
                     /translation="DPVSPPPWFEDRFDVPEPVGDVGGVLVWAFPYDPGLPALPEAAD
                     GRSVRARLPHAAPAAVAVQALTYRPRRRAVFRYRIVGSAGEVLYGKVLRERKLRRALE
                     VMRRLRGRRVRLGTATEAEGLLLSPALPGRSLRDVLVGGGSVPSPARVAGLLGSLEAP
                     GVEGAGPDPVADARATASLIARIVPRAADPAARVAERVERGAAGTPSPVVTVHGDLYE
                     HQVLVDEDFSLGLVDLDDVGPGDLAVDAANFSAHLVALAMAVPAAARRLLAYRSILLP
                     AFAARLSVERRDLAWREALSMLKLSVGPFRVLQPGWPAEVQRRVEVALRLSEGWS"
     gene            993..1670
                     /locus_tag="VF058_05285"
     CDS             993..1670
                     /locus_tag="VF058_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6207756.1"
                     /translation="MSRTHVLLIEDDARIRDIVEQGLGARGFVVTSAPDAETGVDLAG
                     KLDVALILLDLVLPGRQGLDALGTLKELKPDVPVIALTALDDTGSKVGGLDAGVDDYV
                     TKPFSVEELAARIRARLRDRDDGEGVMKAGALTVDLAAHRAVLQGREVALSARELSLL
                     AAFMRHRGQVLSRDQLLRLVWDMDFDPGSNVVEVFVAALRRKLGGGIIETVRGTGYRF
                     VGPQEEA"
     gene            1667..1975
                     /locus_tag="VF058_05290"
     CDS             1667..1975
                     /locus_tag="VF058_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207757.1"
                     /translation="MRIAVGAFLGALLFLAGAVYGSGAIVPESAPPQAVVLVDASAAP
                     AASPEPMPTPTPTDDDEVEEVEHEVEEDDHSGRGRGRGRGGDDDEEDDDDSSGPGDGG
                     "
     gene            1965..3368
                     /locus_tag="VF058_05295"
     CDS             1965..3368
                     /locus_tag="VF058_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6207758.1"
                     /translation="MAAKRSLPLGPRLVLTHVLIAGAVLLVVAALVFHLTRVHLLRGL
                     DARLSIAVQSFQQGPARQVARPADLPDAARSWLAVHGFTSAEVAAVRTSTGPVLSAAG
                     GMPVADLPPEVLDARSPRWWEIEMAGEPVRALTVPLLLGDRTVGTLVVGSSRADIQAT
                     LDALVRGMLIAAGLGLALAAGLGLAAVRRTLRPLRRMTMRTDEIEATGDLSLRVSGDG
                     PRDEVGRLAESFDAMLERLDEAFRSRQRFVADASHELRTPLTVVRGQLELLRDRLEAE
                     GDRASVGLAMDEIDRMRRIVEELLLLARLDEGLPLERAPVEVELLMQEALVRGLAGER
                     RRASVDAEPGLLALADPERLLQVLTNLVANAVKHAGEDARISLRARRADDRVVVEVAD
                     TGRGIPPEELPHVFSRLFRGTRARGEAGAGLGLSIASSLVEAMDGGIRVSSREGAGTV
                     FTVELPLAAPAAAPADV"
     gene            3392..3904
                     /gene="greA"
                     /locus_tag="VF058_05300"
     CDS             3392..3904
                     /gene="greA"
                     /locus_tag="VF058_05300"
                     /inference="COORDINATES: protein motif:HMM:NF015414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX6207759.1"
                     /translation="MTTRPPRRPGEEPTAVLTRDAYERLRSELDRLRGEGRREMAERL
                     ERAREHGDIRENAEYDAAKDAQGLMEARIREVERLLRDPDIVEGAASTEEAGPGVLIT
                     VRPLDDDDDQEERFLLAASKEERAKGARTVSLSSPLGEALAGKKVGEKVTYEAPGGTF
                     AYEVLSLEIP"
     gene            3901..4323
                     /gene="arfB"
                     /locus_tag="VF058_05305"
     CDS             3901..4323
                     /gene="arfB"
                     /locus_tag="VF058_05305"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014911236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX6207760.1"
                     /translation="MNVRVNRRVTIPASELSFRFTPSGGPGGQHANRSATRVELVWNV
                     EESAALGPRQKARVRRALRGRLDAEGNLRLVSGAERSQARNRADVVERLGRLVSGALA
                     VPKRRVRTKPSAAARERRMKAKRRRSEVKRLRRTPPVE"
     gene            4354..4683
                     /locus_tag="VF058_05310"
     CDS             4354..4683
                     /locus_tag="VF058_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX6207761.1"
                     /translation="MSLLIGGTLALLFLPAPWSIVVIVGLAGFEVVEVAFWLWLRRQR
                     PRSGHEALVGRHGVLLEGGRVRIAGTTYPGRVLEGVPGDPVIVQEVDGMTLVVRRDEP
                     RYEEEIA"
BASE COUNT          553 a         1715 c         1879 g          657 t
ORIGIN      
        1 gaccccgtct ctccgccgcc ctggttcgag gatcgcttcg atgtccccga accggtgggc
       61 gacgtgggcg gcgttctcgt gtgggcgttc ccctacgacc cggggctccc cgcgctgccg
      121 gaggccgcag acggccggtc ggtgcgggcc aggctccccc acgccgcgcc ggccgcggtg
      181 gcggtccagg cgctcaccta ccggccccgg cggagggcgg tgttccggta ccggatcgtg
      241 gggagcgccg gcgaggtgct gtacggcaag gtccttcgcg agcggaagct gcgccgggct
      301 ctcgaggtga tgcgccgact ccgcgggcgc agggtccggc tcgggacggc gacggaagcc
      361 gaaggcctgc tcctctcgcc ggccctcccc ggccgctcgc tccgggacgt gctcgtcggc
      421 ggcggctccg tgccgagccc ggcccgcgtc gccggcctcc tggggtcgct ggaggcccca
      481 ggggtggagg gcgccgggcc cgatcccgtg gccgacgctc gggccaccgc ctcgctgatc
      541 gcgcgcatcg tcccccgggc cgcggacccg gccgcccgcg tggccgagcg tgtggagcgt
      601 ggagccgccg ggaccccctc gcccgttgtc accgtgcacg gggacctcta cgagcaccag
      661 gtgctggtgg acgaggactt ctcgctcggg ctggtggacc tggacgacgt cggcccgggc
      721 gacctggcgg tggacgccgc caacttcagc gcgcacctcg tggcgctggc catggccgtt
      781 ccggcggccg cccgccggct cctggcctac cgctcgatcc tgctccccgc gttcgccgcg
      841 aggctcagcg tggagcggcg ggatctcgcg tggcgggagg cgctctcgat gctgaagctg
      901 tccgtgggcc cgttccgcgt cctccagcca gggtggcccg ccgaggtgca acgacgcgtc
      961 gaggtggcgc tacgcttgtc cgaggggtgg tcgtgagccg aacccacgtc ttgctgatcg
     1021 aggacgacgc gcggatccgg gacatcgtgg agcaggggct cggagcgcgc gggttcgtcg
     1081 tgacgagcgc gcccgacgcc gagacggggg tcgacctcgc ggggaagctc gacgtggcgc
     1141 tgatcctcct cgatctcgtg ctgcccggac gccagggcct ggacgccctg gggaccctga
     1201 aggagctcaa gcccgacgtg cccgtgatcg cgctgaccgc gctggacgac acgggctcga
     1261 aggtcggcgg cctcgacgcg ggcgtcgacg actacgtgac gaagcccttc tcggtggagg
     1321 agctggccgc gcggatccgg gcccggctcc gcgaccgcga cgacggcgag ggcgtcatga
     1381 aggcgggcgc cctgaccgtg gacctcgcgg cgcaccgcgc cgtgctccag gggcgggagg
     1441 tggcgctctc ggcgcgggag ctctccctcc tcgccgcgtt catgcgccac cggggccagg
     1501 tcctctcccg cgaccagctg ctccgtctcg tctgggacat ggacttcgat cccggctcca
     1561 acgtggtcga ggtcttcgtg gccgccctcc ggaggaagct cgggggcggg atcatcgaga
     1621 ccgtgcgggg caccggctac cggttcgtgg gtcctcagga ggaggcatga ggatcgcggt
     1681 cggggcgttc ctgggggccc tgctcttcct cgcgggggcc gtgtacggat cgggagcgat
     1741 cgtccccgag agcgcgcctc cccaggccgt cgtcctggtg gacgcctccg cggcgcccgc
     1801 cgcctcaccc gagcccatgc ccacgcccac gccgaccgac gacgatgagg tggaggaggt
     1861 cgagcacgag gtcgaggagg acgaccactc cgggcgcggg cgaggccgtg gccgcggcgg
     1921 cgacgatgac gaggaggacg acgacgactc ctcgggcccg ggcgatggcg gctaagcgct
     1981 cgctccccct cgggccccgc ctcgttctca cgcacgtcct catcgcgggg gcggtgctgc
     2041 tcgtggtcgc cgcgctcgtc ttccacctca cccgggtgca tctgctgcgg gggctcgacg
     2101 cccggctgtc gatcgccgtc cagtcgttcc agcagggccc ggcccgccag gtcgcccgcc
     2161 ccgccgacct ccccgacgcg gccaggagct ggctggccgt ccacgggttc acctccgcgg
     2221 aggtcgcggc ggtccggacg tcgaccgggc cggtgctctc ggcggcgggc ggcatgccgg
     2281 tggccgacct ccccccggag gtcctggacg ctcgctcccc gcgctggtgg gagatcgaga
     2341 tggccggcga gccggtccgg gcgctcaccg tccccctcct gctgggggat cgaacggtgg
     2401 ggaccctcgt cgtgggctca tcgcgcgccg acatccaggc cacgcttgac gccctcgtgc
     2461 ggggcatgct gatcgcggcg ggcctggggc tggccctcgc cgccgggctc ggtctggccg
     2521 ccgtccgccg gaccctgcgg ccgctccgcc gcatgacgat gaggaccgac gagatcgagg
     2581 cgacgggcga cctctcgctg cgcgtctcgg gcgacgggcc gagggacgag gtgggccgtc
     2641 tggcggaatc cttcgacgcg atgctggagc ggctggatga ggcgttccgc tcccggcagc
     2701 gcttcgttgc ggacgcctcc cacgagctgc gcacgccgct caccgtggtg cggggacagc
     2761 tcgagctgct gcgggatcgg ctcgaggccg agggcgaccg ggcctcggtg gggctggcga
     2821 tggacgagat cgaccggatg cgccgcatcg tcgaggagct gctgctgctc gcgcggctgg
     2881 acgaggggct ccccctggag cgggcgcccg tcgaggtcga gctcctgatg caggaggcgc
     2941 tcgtccgggg gctggcgggg gagcgccggc gggcctccgt ggacgccgag cccgggctcc
     3001 tggccctggc cgaccccgag cgcctgctcc aggtcctgac gaacctcgtg gcgaacgcgg
     3061 tcaagcacgc cggcgaggac gcccggatct cgctccgcgc ccggcgcgcg gacgaccggg
     3121 tcgtcgtgga ggtggccgac accgggcgcg gcatccctcc cgaggagctg ccccacgtgt
     3181 tctcccggct gttccgtggc acgagggccc ggggcgaggc cggcgccgga ctcggcctct
     3241 ctatcgcgtc ctcgctggtg gaggcgatgg acgggggcat ccgggtctcg tccagggagg
     3301 gggccggcac cgtcttcacc gtggagctcc cgctcgccgc ccccgccgcg gctccggccg
     3361 atgtctaggg cctttgctac cctcgggggt catgaccacg agacccccac gccgaccggg
     3421 agaggagccc accgcggtcc tcactcggga cgcctacgag cggctccgga gcgagctgga
     3481 ccgcctccgc ggtgagggca ggcgtgagat ggccgagcgg ctggagcgcg cccgggagca
     3541 tggcgacatc cgggagaacg ccgagtacga cgcggccaag gacgcgcagg gcctgatgga
     3601 ggcccggatc cgcgaggtcg agcgcctcct ccgcgacccg gacatcgtcg agggggccgc
     3661 gtccaccgag gaggccggcc cgggcgtcct gatcaccgtc cgcccgctgg acgacgacga
     3721 cgaccaggag gagcggttcc tgctcgcggc ctccaaggag gagagggcca agggggcccg
     3781 caccgtgtcg ctctcgtccc cgctggggga ggccctggcc gggaagaagg tcggggagaa
     3841 ggtcacctac gaggcccccg ggggaacctt cgcctacgag gtcctgtcgc tcgagatccc
     3901 gtgaacgtcc gggtcaaccg gcgcgtcacg atccccgcct ccgagctctc gttccggttc
     3961 actccgtccg gaggacccgg cggccagcac gcgaaccgct cggccacccg ggtcgagctg
     4021 gtctggaacg tcgaggagag cgcggcgctg gggccccggc agaaggcgcg cgtccgccgc
     4081 gcgctgcggg ggaggctcga cgcggagggc aacctgcggc tcgtctccgg cgcggagcgg
     4141 tcgcaggctc ggaaccgggc cgacgtcgtg gagcggctcg gccgtctggt ctcgggggcg
     4201 ctcgccgtgc ccaagcgccg ggtgcggacg aagccctcgg cggcggcgcg ggagcgtcgc
     4261 atgaaggcga agaggcggcg gagcgaggtg aagcggctcc gccggacgcc tcccgtggag
     4321 tgacccggcc gggcacggta cgctcggtcc gtcatgtcgc tgctgatcgg ggggacgctc
     4381 gcgctcctct tcctgccggc tccctggtcg atcgtcgtca tcgtgggcct ggccgggttc
     4441 gaggtcgtgg aggtggcctt ctggctctgg ctccggaggc agcgtccccg ctcggggcac
     4501 gaggcgctcg tggggaggca cggtgtgctg ctcgagggcg gccgcgtccg gatcgcgggg
     4561 acgacctacc cgggccgggt gctcgagggt gtgcctgggg acccggtgat cgtgcaggag
     4621 gtcgacggga tgaccttggt cgtccgcagg gacgagcctc ggtacgagga ggagatcgcg
     4681 tgagcgggca cccgctcgag ccgacggggg ccgagctccg ggcgatggtc gaggaggcgg
     4741 tgtcgtacct gtcgtcgttc ctggacgagc tgccgggggc gccggcggcc ggcgcgcccg
     4801 acaa
//
LOCUS       DASUTW010000158        15238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_308868, whole genome shotgun sequence.
ACCESSION   DASUTW010000158 DASUTW010000000
VERSION     DASUTW010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15238
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308868"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VF058_05315"
     CDS             complement(<1..282)
                     /locus_tag="VF058_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207762.1"
                     /translation="MTGTELIVLLAALGVAGTAVLIALRRPAPPQADTRVPAILEAQQ
                     SRLDRLADALGRQTVDDQELRRSLEATKRTVESMRTHAEARRQADEQTWE"
     gene            complement(336..1265)
                     /locus_tag="VF058_05320"
     CDS             complement(336..1265)
                     /locus_tag="VF058_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6207763.1"
                     /translation="MGLLDGKVIAVTGAGRGIGREEALLLAREGARVVVNDLGGEWDG
                     TGEDTRVAAEVASEIRAAGGEAEAHFEDISEPGGARSLVETALDRWDRIDGLVNNAGI
                     LRDRMSFNMAPEEWDAVIKVHLRGTFLCTAEVCRRWRELAKEGEDVSGRRIVNTSSTS
                     GLLGMVGQSNYGTAKAGIAAFTQIVSMEMRSYGVGVNAIAPGARTRMTEKTFGDLQVE
                     GDFDPLDPGNIAPLVAFLCSDAAAGITGQVFGITGGLVELYQSWTPVSELDKGARWEA
                     SELAERMDELFGERPRSYEPGRSPLRRITGIGG"
     gene            1382..2821
                     /locus_tag="VF058_05325"
     CDS             1382..2821
                     /locus_tag="VF058_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX6207764.1"
                     /translation="MLDIARAAVEAARGAGAGYADARAALRETESLTVKNQEMEGVDR
                     SRSMGVGIRVLVDGFWGFASTDRREPEEVERAAVLAVEIARAASRLPREPVALAEVEP
                     VVDVWQTVVAEDPFTIPLDEKVSLLMEASRRTQGVRGVSFAEASLDFFRQRTAFASSE
                     GAAIDQTITHSGGGIEATAISGGELQRRSFPNSFRGHFSAAGYEHIRSLGLVEEAERI
                     GEETVALLEAPDCPSIETTLILDASQMVLQVHESVGHPIELDRVLGMEEAYAGTSFLT
                     PEDRGRLRYASGVVTISADATVPTGIGSFGYDDEGVPAQPVPVIEEGIFREFLSSRET
                     AAALGEASNGTMRADGWEHLPLIRMTNVNLHPGEGTLAELIGDTKDGIFMSTNSSWSI
                     DDKRVNFQFGCEAAWRIEDGRLTQLYRNPNYTGITTEFWRSCDAVCGSEEWTMWGTPN
                     CGKGQPGQVARVGHGTSPARFQNVRVGVR"
     gene            2821..4170
                     /locus_tag="VF058_05330"
     CDS             2821..4170
                     /locus_tag="VF058_05330"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX6207765.1"
                     /translation="MPEPMGRDAALELAGPALDGAGGADVEVLVIHGWGGLTRFAGSA
                     IHQSTAQEETAVRVRAVKDGRVGVSSTNDLRPAGVQAAARSALEMVELAAPDPLFPGL
                     APRAEAPTIDGYDPATSETSPAARAEAVEALVGACGPGFTAAGAFETGALEVAVANTE
                     GLTVHATRSKASITAVVSGGEGGAGFAERAAGRVADLDAAAVGAAAFAKARDSQRPRD
                     LQPGDYEVVLEPAAVATLLAFLSYLGFGGRAIAEGRSCFAGREGQRLMSEQVSIWDDA
                     LSPDTIGLPFDFEGTPKQRVDLVREGIFVGGVHDRRSGAQTGGESTGHALPPPNPEGP
                     FPLNLFMATGNASTDDMIAATRRGLLVSRFHYANVVHPKEAVITGMTRDGTWLIEDGA
                     IVGPVKNLRFTESIIDALDRVDLVGSESTLASEFFFEASRVPALRIGSFRFTGTSDH"
     gene            complement(4148..6943)
                     /locus_tag="VF058_05335"
     CDS             complement(4148..6943)
                     /locus_tag="VF058_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6207766.1"
                     /translation="MTSTERSLLAAGTLLAGSTAVLSLTSRGPAAGTTPLPWVALAGF
                     LLVLAAWVALERRARPDAPSGRSGGREAILGAVAEAAERFLSEGVEAMPVVLEQLAGA
                     TGASRVYVWENHEGEDGALLTSQRYEWVAPGIDSQIDNPDLQALPYEEAGLGRWVRRL
                     GAGMTIHGPLRAFPSRERAVLDPQRIRSLVAVPIFVGDEWWGFIGVDDCVRERAWSVG
                     EIDALRVAAGALGGAIHRARAVELLEQAEAKYRAIVEKIPAAIFLDALDGKATTLWVS
                     PQIEEILGFPAEEWLADPDLWLRQMHPADRDRIVAATVRHNETLEPFGEEYRIFHRDG
                     RVVWVRDEAVITYDDEGRPRFSQGFFMDVTERKRAEAQERRHAEYLEAIHETALGLMD
                     RLEVDGLLEAIVGRAAELLGTGHGFVYLPDENGERMEMKVGVGALTGAVGLKAGRGEG
                     AAGLVWERGEPVVISDYAGWEGRLSFSVDVSSVMAVPLGSRNEVVGVLGLAHMDPEVG
                     FTDEDVEHLATFAEFASIALDNARLYEAARRELAERKRAQAELEEQEERFRTLSDSAL
                     EGICIHDRGTIIEVNRAFAEMFRLPPEELVGTQVLELVAPEDREAAAWRIDEQSEEVG
                     RHEGIRGDGSRFAVELVGRRIPYRGREARVVAIRDITDRRRAEESLREAYEREREAAE
                     QLRHVDEMKNAFLTAVSHELRTPHSAILGFALTLDRESLSLSPAEAHEMIDRIVANAR
                     KRDSLLTDLLDLDRVSRGILEATRRPTDVGDLVHRVVEGADLTGRRVLVEAQELVAHV
                     DGPKVERIVENLLANAAKYTPPGTPVWVRLVRRPGGLLLAVEDAGPGVELEQRDAIFR
                     PFTQGEDAPAHAPGTGIGLSLVARFAEMHGGSAWVEDREGGGASFRVFLPTAEEDQSS
                     ELPRAVGFEGLSDRSSR"
     gene            7065..8003
                     /locus_tag="VF058_05340"
     CDS             7065..8003
                     /locus_tag="VF058_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207767.1"
                     /translation="MALALILVAAGSLLLYGGAELTDRGLSRVSIPGVPALGPRAIRV
                     GAEIGVLAAALAAAGRGETEIAGGLATGGALFLLAVGMGWRLAVGQRPLVAPSSTLLL
                     LAPSGAVILAALAVSGRYVDRLEGLLLAVGFAGYLVLRLREHADLSGGEEPPREGAPA
                     REPDPESDGARSAAPAVVGMAILTGGAVVIVEGGVRLSLAGNLAAGFVGASLLAALTT
                     LHRAIPRSLGGEPPEHAAGSLLGSITALLAGVLGLAALVRPLTIDTGVNLAFLGVAVL
                     SAVVAVALLLRRRAGWILGVALLASYATWLVYASGA"
     gene            complement(8000..9118)
                     /locus_tag="VF058_05345"
     CDS             complement(8000..9118)
                     /locus_tag="VF058_05345"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017237832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6207768.1"
                     /translation="MRPLQGIRVADLSRVLAGPLCTMVLADLGAEVVKVERPGVGDET
                     RSWGPPFVGDDAAYFLSVNRGKRSLELDLKAPAGAAAARELILRSDVLVENFRPGTMA
                     RLGLDPEELRERQPGLVVCSITAMGEDADPRVAGYDLVMQAATGLMSLTGPVGGEPTK
                     VGVALLDVVAGLYAAVGITAALRERERTGRGAHVSVSLFEAGVAALVNQAANYLLGGE
                     VPIPMGNAHPNIAPYEALSAPGGPLVLAAANDGLFVRACEVLARPDLPSEARFARNAD
                     RVRNREALRGLLEEALADRPRDEWVRLLTEAGVPAAPVRDLAAVFGSEEGRATIQEID
                     DPERGLLRLVRSPIRLDGVAESESRPPPLLGMHEPDDL"
     gene            complement(9115..10707)
                     /locus_tag="VF058_05350"
     CDS             complement(9115..10707)
                     /locus_tag="VF058_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6207769.1"
                     /translation="MARAADEVPTGRHLESFDPATGELVGRVPRTPPEEVEAVTVSVA
                     EAQPTWARVSLSDRLDVIGRAADRVLDRQEELSLLFTRESGKPITESSIAEVGAAALV
                     LSWVARHGHRHLSPERVPTSQPIVAHKKHWIVFDPLGVIGVIGPWNYPCLLPMADVAF
                     ALAAGNGVVFKPSELTPLSGQAIADLFAEAGLPQGVLRVVHGEGDTGAALCEAGPVRK
                     ILFTGSVESGRKVLEETTRAGKMAQLELGGNDPAMVCVDADLDRAVAGVLWASCANAG
                     QTCAAIERVYVDRRIHDRFVARLVAAAEALVPGDPKRPDTQMGPIISDRQHRRVLEHL
                     EDARRRGATVECGGPVEVPGLAGRFVAPAVLTGADHSMRVMREETFGPLIPVMAFDDE
                     REALALANDSEFGLGASVWTRDPRRGRALARRIEAGMVWINDHAYSNGLGEAPWGGVK
                     SSGTGVAHSRYGLQEMTQKRLIAEDAGRVPAGWWFPYSETRRRGFLAVVESLARTRRA
                     RRVRAAWDGRGTIGRYLRSLLR"
     gene            complement(10723..11556)
                     /locus_tag="VF058_05355"
     CDS             complement(10723..11556)
                     /locus_tag="VF058_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015796371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX6207770.1"
                     /translation="MSIDLHTHTRHSDGTFEPRESVSLALDRGLRILAVTDHDTMAGV
                     SAAEEAAAGTGLEIVPGVEFSAEYEGGSIHVLGYWMDPGHEEFRAELARLQDDRIRRG
                     ERMVERLQALGHPVTVERVREIAAGGSVGRPHVAQALVEAGVVPAIEDAFTADLIGTG
                     GRAYVQKHALHPVEAVRLIRRADGLAVLAHPVHWRDGVPVPHPLIREMIEAGMDGIEC
                     AHPDQDRATEARYRELSARYGLVATGSSDCHGTRYDPIRMGSRTTDLEEFEKLLARRR
                     R"
     gene            11650..11853
                     /locus_tag="VF058_05360"
     CDS             11650..11853
                     /locus_tag="VF058_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207771.1"
                     /translation="MATTAERLREPDMLRSYLHALARSGGSGDQDLGRGRQRRVCAQC
                     GEQAVFSPETDGWYRCGHCGRYA"
     gene            complement(11854..13983)
                     /locus_tag="VF058_05365"
     CDS             complement(11854..13983)
                     /locus_tag="VF058_05365"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEX6207772.1"
                     /translation="MARPPATVDIREVQVIKHDRSFFVSNRFGDVPEGNTAALGLYHA
                     DTRFLSRLEVTLNQLRPLLLHSSTERNYSQIVELVFPVRHVFLAGPERKDNISLQRRR
                     VLHRSLFERMRIRNFGLQDRRIGLTVSFESDFLDIFEVRGLAREERGQMLPPEVDRHS
                     VILGYRGLDGVERSTTLRFSPAPETLSEREASFDLAVAAGEQVDIVLEIEPHVGDAAP
                     TRRSMREIEQELEQEYAAWEKRATRFDATDGRIKGFLRRAVRDLRMLTSTGENGTRFI
                     DAGIPWYSAMFGRDSLITAYQALTGCPQLAWSTLRGLAALQGKIDDPWREEEPGKILH
                     ELRVGELAGADEIPHTPYYGSIDATPLWLVVLGAAFRWTGDREALQELWPNALAALRW
                     IDEHGDRDGDGYVEYERRSPRGLDNQGWKDSWDGVPWPDGTPVEPPIALVEVQGYVYQ
                     AKSLMAPLAREMGDEDLAARLEREAAELKGRFNRDFWVEETGSFALALDGEKRRVPTI
                     TSNPGHGLWSRIVDRRLAPHLVKRLMDPGMLSGWGIRTLSSRHSAYDPIGYHTGTIWP
                     HDNSLIAQGLKRYGFDAEAREVLDQLTAAGRHFPYSRFPEVFCGFSSDEVPVPVEYPV
                     ACRPQAWASGAPLLMVRAYGGLSADATRRRLTIERPRLPDWLTGIELRGMRVAGARLD
                     LSFVNSDGVTAVKVDHKRGDLEVVIRQ"
     gene            14044..14286
                     /locus_tag="VF058_05370"
     CDS             14044..14286
                     /locus_tag="VF058_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207773.1"
                     /translation="MGSVMGADRFYDAGEIGCGGPTLKEIGRLVDGLPAGGNVEVRST
                     THTGRNSLRAFCRLRELAIEAEDAGPNRDRILIRKT"
     gene            14297..14653
                     /locus_tag="VF058_05375"
     CDS             14297..14653
                     /locus_tag="VF058_05375"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEX6207774.1"
                     /translation="MTIVIHASHGAEDPERATLPFVLGNNAAVAGQDPVILLTVEAVH
                     LATEGGAEGIQKQGLPSLPEIMAEFVSNGGRIWACQSCTTPRGIEEGDLAEGARIGTS
                     MEVVELLAGGAASFAF"
     gene            14693..>15238
                     /locus_tag="VF058_05380"
     CDS             14693..>15238
                     /locus_tag="VF058_05380"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207775.1"
                     /translation="MTVFGAGAAGTALAIHLARKGEDAVLWGSEHDAAVLGDLTEKRR
                     HPALPERLPEELRILGPEDLEEAGAHAEIAVLGANSAGAGPLGRLVAPALEGARLAVS
                     IAKGLQPGTLERVSVVYARELPTIAVVAMSGPSLAAEIAEGLLTAVAFACEDEGACKE
                     AAEAFRSDRFLVSTTDDVVGVE"
BASE COUNT         2095 a         5647 c         5361 g         2135 t
ORIGIN      
        1 ctcccacgtc tgctcgtcgg cctgccgacg ggcctcagcg tgggtccgca tcgactcgac
       61 cgtgcgcttc gtggcctcca gggaccggcg cagctcctga tcgtccacgg tctggcgacc
      121 cagcgcgtcg gccagccggt ccagccggct ctgctgcgcc tccagtatcg cgggcacgcg
      181 cgtgtccgcc tggggcggcg ccggccgacg cagggctatc agcacggccg tccccgcgac
      241 ccccagggcg gccagcagca cgatcagctc ggttccggtc atcgcgacct cctctcgcga
      301 acactctaga gagagggtct gacacgaacg gcggctcagc cgccgatgcc ggtgatccgc
      361 cgcaggggcg agcggccggg ctcgtacgag cgcggccgct cgccgaagag ctcgtccatc
      421 cgctcggcga gctcggaggc ctcccaccgg gcccccttgt cgagctccga gacgggcgtc
      481 cagctctggt agagctcgac aaggccgccc gtgatgccga agacctggcc ggtgatcccg
      541 gccgccgcgt ccgagcagag gaacgccacg agcggcgcga tgttgccggg atcgagcggg
      601 tcgaagtcac cctccacctg aaggtcgccg aaggtcttct cggtcatgcg ggtgcgggcg
      661 ccgggcgcga tcgcgttcac gcccacgccg tagctccgca tctccatcga cacgatctgc
      721 gtgaatgcgg cgattccggc cttggccgtg ccgtagttgg actgccccac catccccagg
      781 agcccggacg tgctggaggt gttgacgatg cggcgacccg agacgtcctc gccttctttg
      841 gccagctcgc gccagcggcg gcagacctcg gcggtgcaca ggaaggtccc ccggaggtgg
      901 accttgatca ccgcgtccca ctcctcgggc gccatgttga agctcatgcg gtcgcggagg
      961 atgccggcgt tgttgacgag gccgtcgatg cggtcccagc gatccagcgc ggtctcgacg
     1021 agcgagcggg cgcccccggg ctcggagatg tcctcgaagt gggcctcggc ctccccgccg
     1081 gccgcccgga tctcgctcgc cacctcggcg gcgacgcgag tgtcctcgcc ggtgccgtcc
     1141 cactcgcccc cgaggtcgtt gacgacgacc cgtgcgccct cccgggccag cagcagcgcc
     1201 tcctccctgc cgatcccccg accggcgccg gtgacggcga tcaccttccc gtcgagcaag
     1261 cccatgcatc gctcctttcc gatcgcccac acgatagacg aagcctctcg gtcgaggacg
     1321 gccgcgtcgg atacactgcg ccgggttccg cgccgaggcg ggttcgtccg gaaggagagg
     1381 catgctcgac atcgcgaggg cggcggtcga agcggcgcgc ggcgcgggcg ccggctacgc
     1441 cgacgcccgc gccgcgctcc gcgagaccga gtcgctcacg gtcaagaacc aggagatgga
     1501 gggcgtggac cgctctcgct ccatgggggt ggggatccgg gtcctcgtgg acggcttctg
     1561 ggggttcgcc tccaccgacc gccgggagcc cgaggaggtc gagcgcgccg cggtcttggc
     1621 ggtggagatc gcccgggccg cctcccgcct cccccgcgag ccggtcgctc tggccgaggt
     1681 cgagcccgtc gtcgacgtgt ggcagaccgt cgtggccgag gaccctttca cgatccccct
     1741 cgacgagaag gtctcgctgc tcatggaggc ctcgcgccgg acgcagggcg tccggggcgt
     1801 gtcgttcgcg gaggccagcc tggacttctt ccgtcagcgg acggcgttcg cctcgtcgga
     1861 gggcgccgcc atcgaccaga cgatcaccca ctccggagga gggatcgaag ccacggcgat
     1921 ctccggcggc gagctgcagc gccgctcgtt ccccaactcg ttccgggggc acttctcggc
     1981 cgccggctac gagcacatcc ggtccctggg gctggtggag gaggccgagc ggatcggcga
     2041 ggagacggtg gccctgctcg aggcgcccga ctgcccgtcg atcgagacca cgctgatcct
     2101 cgacgccagc cagatggtgc tccaggtcca cgaatcggtg ggccacccga tcgagctcga
     2161 tcgcgtcctc gggatggagg aggcgtacgc cgggacctcc ttcctcaccc ccgaggatcg
     2221 cgggcggctc cggtacgcct cgggcgtcgt gacgatctcg gccgacgcca ccgtccccac
     2281 cgggatcggg tcgttcggct acgacgacga gggcgtgccc gcccaaccgg tccctgtcat
     2341 cgaggagggc atcttccggg agttcctctc cagccgcgag accgccgctg cgctcggcga
     2401 ggcctcgaac gggacgatgc gggccgacgg ctgggagcac ctcccgctga tccgcatgac
     2461 gaacgtgaat ctccatcccg gcgagggaac gctggccgag ctgatcggcg acacgaagga
     2521 cggcatcttc atgtccacga acagctcgtg gtcgatcgac gacaagcgcg tcaacttcca
     2581 gttcggctgc gaggcggcct ggaggatcga ggacggccgc ctgacccagc tgtaccggaa
     2641 cccgaactac acgggcatca cgaccgagtt ctggcgctcc tgcgacgcgg tctgcggcag
     2701 cgaggagtgg acgatgtggg ggacgccgaa ctgcggcaag ggccagcccg ggcaggtcgc
     2761 tcgcgtgggg cacggcacct cgcccgcccg cttccagaac gttcgggtgg gggtgcgctg
     2821 atgcccgagc cgatggggcg cgacgccgcg ctggagctcg cggggcccgc gctcgacggc
     2881 gccggcggcg cggacgtcga ggtcctcgtg atccacgggt ggggaggcct gacccgattc
     2941 gccggatcgg cgatccacca gagcacggcc caggaggaga cggcggtccg agtccgcgcc
     3001 gtcaaggacg gccgcgtcgg cgtgtcctcg acgaacgacc tccgcccggc cggcgttcag
     3061 gccgcggcgc ggagtgcgct ggagatggtt gagctggccg cgcccgaccc gctgttcccg
     3121 gggctcgccc ctcgggccga ggctccgacg atcgatggct acgaccccgc cacgtccgag
     3181 acgtcgcccg cggccagggc cgaggccgtc gaggcgctcg tgggggcctg cgggcccggc
     3241 ttcacggccg cgggggcgtt cgagacgggt gccctcgagg tggcggtggc caacacggag
     3301 gggctgaccg tccacgccac gcggtccaag gcttcgatca cggcggtcgt ctccggcggc
     3361 gagggaggcg cggggttcgc cgagcgagcc gccggacgag tggccgacct ggacgccgcg
     3421 gcggtgggag cggcggcctt cgccaaggcc cgcgacagcc agcggccccg ggacctccag
     3481 ccgggagact acgaggtggt gctggagccc gccgccgtgg ccacgctcct cgccttcctc
     3541 tcctacctcg ggttcggggg acgggccatc gccgagggcc gctcgtgctt cgccggcagg
     3601 gaaggccagc ggctgatgtc cgagcaggtc tcgatctggg acgacgccct gtccccggac
     3661 acgatcgggc tccccttcga cttcgagggg acgcccaaac aacgcgtgga cctcgtgcgc
     3721 gaggggatct tcgtcggagg cgtccacgat cgccgcagcg gggcgcagac cgggggcgaa
     3781 tccaccggcc acgcgctccc gcctcccaac cccgaggggc cgttcccgct caacctgttc
     3841 atggcgaccg gcaacgcgtc cacggacgac atgatcgccg ccacccgccg gggcctcctc
     3901 gtcagccgct tccactacgc gaacgtggtc caccccaagg aggccgtgat caccgggatg
     3961 acccgggacg gcacctggct gatcgaggac ggcgccatcg tgggtcccgt gaagaacctc
     4021 cgcttcaccg agtcgatcat cgacgccctc gaccgcgtcg acctggtcgg ctccgagtcc
     4081 acgctcgcca gcgagttctt cttcgaggcc tcacgcgtgc cggcgctcag gatcggctcg
     4141 ttccggttca ccgggacgag cgatcactga gcccctcgaa cccgacggcc cggggcagct
     4201 cggaggactg gtcctcctcg gccgtgggga ggaacacccg gaacgacgcc ccgcctccct
     4261 cgcgatcctc gacccaggcg ctcccgccgt gcatctcggc gaaccgagcc acgagggaga
     4321 gcccgatccc cgtcccgggg gcgtgggccg gggcgtcctc gccctgggtg aagggccgga
     4381 agatcgcgtc ccgctgctcc agctccacgc ccggccccgc gtcctccacg gcgagcagca
     4441 gcccgccggg cctccggacc agccgcaccc agaccggggt cccgggaggc gtgtacttcg
     4501 ccgcgttcgc cagcaggttc tccacgatcc gctccacctt cggcccgtcc acgtgggcga
     4561 cgagctcctg ggcctcgacg agcacgcgtc ggccggtgag gtcggctcct tccaccacgc
     4621 ggtggacgag gtcgcccacg tccgtcggcc gccgcgtcgc ctcgaggatc ccgcgagaga
     4681 cgcgatcgag gtcgaggagg tccgtcagca gcgagtcgcg cttcctggcg ttggccacga
     4741 tccgatcgat catctcgtgc gcctcggccg gggagaggct gaggctctcc cgatccagcg
     4801 tcagcgcgaa ccccaagatg gcggagtgcg gggttcggag ctcgtggctc accgcggtga
     4861 ggaaggcgtt cttcatctcg tcgacgtggc ggagctgctc cgccgcctca cgctcccgct
     4921 cgtaggcttc gcgcagggac tcctcggccc gtcgccgatc ggtgatgtcc cggatcgcca
     4981 cgacccgggc ctccctccct cggtagggga tcctgcgtcc cacgagctct acggcgaagc
     5041 gggacccgtc gccccggatc ccctcgtgcc gcccgacctc ctccgactgc tcgtcgatcc
     5101 gccaggcggc cgcctcgcga tcctcgggcg ccacgagctc caggacctgg gtccccacga
     5161 gctcctcggg tggcaggcgg aacatctcgg cgaaggcccg gttgacctcg atgatcgtcc
     5221 cccggtcgtg gatgcagatg ccctccagag ccgagtccga tagcgtgcgg aagcgctcct
     5281 cctgctcctc caactcggcc tgggcccgtt tccgctcggc gagctcgcgc cgcgcggcct
     5341 cgtacagccg cgcgttgtcc agggcgatcg aggcgaactc cgcgaaggtc gccaggtgct
     5401 ccacgtcctc gtcggtgaag ccgacctccg gatccatgtg ggcgaggccc aggaccccca
     5461 ccacctcgtt ccgggatccg agcgggaccg ccatcacgct cgacacgtcg acggagaagc
     5521 tcaggcggcc ctcccagccc gcgtagtccg agatcacgac cggctcccct cgctcccaga
     5581 ccagcccggc cgcaccctca ccccgcccgg ccttcagccc caccgcgccc gtgagggccc
     5641 ccacgccgac cttcatctcc atccgctcgc cgttctcgtc ggggaggtag acgaacccgt
     5701 gtcccgtgcc gagcagctcc gccgcccgcc ccacgatcgc ctccaggagg ccgtcgacct
     5761 cgaggcgatc catgagaccg agcgcggtct catggatcgc ctcgaggtac tccgcgtggc
     5821 gccgctcctg cgcctccgcg cgcttccgct cggtgacgtc catgaagaag ccctgcgaga
     5881 accgcggcct gccctcgtcg tcgtaggtga tcacggcctc gtcccgcacc cacaccaccc
     5941 ggccgtcgcg gtggaagatc cggtactcct ccccgaaggg ctccagggtc tcgttgtggc
     6001 ggacggtggc ggcgacgatg cggtcccggt cggccgggtg catctgccgc agccacagat
     6061 cggggtcggc cagccactcc tcggccggga accccaggat ctcctcgatc tgggggctga
     6121 cccagagcgt ggtggccttc ccgtcgaggg cgtccaggaa gatggcggcg gggatcttct
     6181 ccacgatcgc ccggtacttc gcctcggcct gctccagcag ctccaccgct ctcgctcgat
     6241 ggatcgcccc gccgagggcg cccgccgcca ctctcagggc gtcgatctct ccaacggacc
     6301 acgcgcgctc cctcacgcag tcgtccacgc cgatgaagcc ccaccactcg tcgcccacga
     6361 agatggggac ggcgacgagc gagcggatcc gctggggatc gaggaccgcg cgctcccgtg
     6421 acgggaacgc ccggaggggc ccgtggatcg tcatcccagc gcccaggcga cggacccagc
     6481 gacccaggcc cgcctcctcg tacggcaggg cctgcaggtc ggggttgtcg atctgcgagt
     6541 cgatgccggg ggccacccac tcgtagcgct gtgaggtgag cagcgcaccg tcctcgccct
     6601 cgtggttctc ccaaacgtag acgcggctgg cgcccgtcgc ccccgcgagc tgctcgagga
     6661 ccacgggcat cgcctccacg ccctcgctga ggaagcgctc ggcggcctcc gccacggccc
     6721 cgaggatcgc ctcgcgccct cccgaacgtc cggacggagc gtcggggcgg gcccgacgct
     6781 cgagggcgac ccacgcagcg aggacgagga ggaacccagc gagggccacc cacgggagcg
     6841 gcgtggtccc ggctgcggga cctcgggacg tcagcgagag cacggcggtg gagccggcga
     6901 gcagcgtacc ggcggcgagg aggctccgct cggtcgaggt catctctcaa tgaggttgtc
     6961 ggaagcgatc ggggcgtccc tccatggtca tttcgagcca tcttccgggc cgccggatgg
     7021 gcgacgcgtc ccggacggac cggctcatcc tctatcgtgc gcgcatggcc ctcgccctaa
     7081 tcctggtcgc ggccggctcc ctgctcctct acggaggggc cgagctcacc gaccggggcc
     7141 tgtcccgcgt ctcgatcccc ggtgtcccgg ccctcgggcc gcgggcgatc cgggtcgggg
     7201 ccgagatcgg cgtgctcgcg gcggcgctgg cggccgccgg gcgtggcgag accgagatcg
     7261 cagggggcct ggccaccggc ggggcgctgt tcctgctcgc ggtggggatg gggtggaggc
     7321 tcgccgtcgg ccagcggccc ctcgtcgcgc catcctcgac gctcctgctg ctggcccctt
     7381 ccggagccgt gatcctggcc gcgctcgccg tgagcggtcg ctacgtggac cgcctcgagg
     7441 ggctgctgct ggcggtgggg ttcgccgggt acctcgtcct tcgactgagg gagcacgcgg
     7501 acctgtcggg aggcgaggag cccccacggg agggcgcccc ggcacgggag ccggatccag
     7561 agtcggacgg ggcgcgatcg gcagctccgg cggtcgtggg gatggcgatc ctgaccggcg
     7621 gcgccgtggt gatcgtggag ggaggggtcc ggctctcgct ggccgggaat ctggccgcgg
     7681 gcttcgtggg agcctctctg ctggccgccc tcacgacgct ccaccgcgcg atcccacgat
     7741 ccctcggagg ggagcctccc gaacacgccg ccggaagcct cctcgggtcg atcaccgccc
     7801 tgctcgccgg ggtcctgggg ctggccgccc tggtccggcc gctcacgatc gatacggggg
     7861 tcaacctcgc gttcctgggg gtcgccgtgc tctccgcggt cgtggccgtc gccctgctgc
     7921 tgcggcgtcg agccgggtgg atcctcgggg tcgccctcct cgcctcctac gcgacctggc
     7981 tcgtctacgc gtccggagcc tagaggtcgt cgggctcgtg catgcccagc aggggcggtg
     8041 ggcgcgattc cgactcggcc acgccgtcga gccggatcgg gctccgcacg agccgcagga
     8101 ggccgcgctc gggatcgtcg atctcctgga tcgtggcccg gccttcctcc gacccgaaga
     8161 cggcggcgag gtcgcgcact ggggccgcgg ggaccccggc ctcggtcagc agacggaccc
     8221 actcgtccct cggacggtcg gcgagcgcct cctcgaggag gccgcgaagg gcctctcggt
     8281 tccgcacgcg gtcggcgttg cgcgcgaacc ttgcttccga cgggaggtcc ggccgtgcca
     8341 gcacctcgca tgcgcggacg aagagaccgt cgttcgccgc ggcgagcacg agcggccccc
     8401 cgggcgccga gagggcctcg tagggagcga tgttggggtg cgcgttcccc atcgggatcg
     8461 gcacctcacc cccgagcagg tagttggcgg cctggttgac cagcgcggcc accccggcct
     8521 cgaagagcga gacggacacg tgcgcccccc gccccgtccg ctcgcgctcg cgcagcgcgg
     8581 cggtgatccc cacggccgcg tagaggccgg ccaccacgtc cagcagcgcg acgccgacct
     8641 tcgtgggctc gccgccgacc ggccccgtca ggctcatgag cccggtcgcc gcctgcatca
     8701 cgaggtcgta gccggccacc cgggggtcgg catcctcgcc catggccgtg atcgagcaca
     8761 cgaccaggcc cggttggcgc tcgcgcagct cctcgggatc gagccccagc cgagccatcg
     8821 tccccgggcg gaagttctcc acgaggacgt ccgatcggag gatcagctcc ctggccgcgg
     8881 cggctcccgc cggcgccttg agatcgagct cgagcgagcg cttcccccgg ttcaccgaca
     8941 ggaagtacgc ggcgtcgtcc ccgacgaacg gcggccccca ggagcgggtc tcgtcgccca
     9001 cccccggccg ctcgaccttc accacctccg cccccagatc ggccaggacc atcgtgcaga
     9061 gcggccccgc caggacgcgg gacagatcgg cgacccggat cccctggagc ggcctcacct
     9121 gagcagggac cgcaggtatc gcccgatcgt gccccgcccg tcccacgccg cgcgcacccg
     9181 ccgcgcgcgc cgggtccggg ccagcgactc cacgacggcc aggaagccgc gcctgcgggt
     9241 ctcgctgtac gggaaccacc agccggcggg gacccgcccg gcgtcctcgg cgatcagccg
     9301 cttctgggtc atctcctgga gaccgtacct ggagtgcgcg accccggtcc ccgaggactt
     9361 cacgccaccc cacggcgcct cacccagccc gttcgagtac gcgtggtcgt tgatccagac
     9421 catgccggcc tcgatccgcc gggccagggc gcgtcccctc cgcgggtcgc gggtccacac
     9481 gctcgccccc agcccgaact cggagtcgtt ggccagcgcc agcgcctccc gctcgtcgtc
     9541 gaacgccatc accgggatca gcggcccgaa cgtctcctcc cgcatgaccc gcatcgagtg
     9601 atccgccccg gtcagcacgg cgggggccac gaaacgcccc gccagccccg gcacctcgac
     9661 cggtcccccg cactcgacgg ttgcgccccg gcgccgggcg tcctccaggt gctccagcac
     9721 ccgccggtgc tggcggtcgg agatgatcgg ccccatctgg gtgtccggac gcttcgggtc
     9781 gccgggcacg agcgcctccg ccgccgcgac gagacgggcc acgaaccggt cgtggatgcg
     9841 ccggtcgacg tagacgcgct cgatcgcagc gcaggtctgc ccggcgttcg cgcacgaggc
     9901 ccagaggacc ccggccacgg cgcggtcgag gtcggcgtcc acgcagacca tcgcgggatc
     9961 gttccccccg agctccagct gggccatctt gcccgcccgg gtcgtctcct cgaggacctt
    10021 ccgcccgctc tccacgctcc ccgtgaagag gatcttccgg accggacccg cctcgcagag
    10081 cgcggccccc gtgtcgccct ccccgtgcac gacgcgcagc acgccctggg gcagcccggc
    10141 ctccgcgaag aggtcggcga tggcctggcc cgagagcggg gtgagctcgg acggcttgaa
    10201 gaccactccg ttgcccgcgg ccagggcgaa ggccacatcg gccatcggga gcaggcacgg
    10261 gtagttccac ggcccgatga cgccgatcac gcccagggga tcgaagacga tccagtgctt
    10321 cttgtgcgcc acgatcggct gcgaggtggg gacgcgctcg ggcgagagat gacgatgccc
    10381 gtgtcgggcg acccatgaga ggacgagggc cgctgcgccc acctcggcga tcgaggactc
    10441 ggtgatgggc ttccccgact ccctggtgaa gaggaggctg agctcctcct gccggtcgag
    10501 cacgcggtcg gccgctcggc cgatcacgtc caggcgatcg ctcagggaca cgcgggccca
    10561 ggtcggctgg gcctcggcca ccgagaccgt cacggcctcg acctcctccg gcggcgtgcg
    10621 gggaacccgc cccacgagct cgcccgtggc ggggtcgaag ctctccagat gcctcccggt
    10681 cgggacctca tcggcggccc gagccatcat cctcctcccg actcagcgtc ggcggcgggc
    10741 cagcagcttc tcgaactcct cgagatcggt ggtccgcgat cccatgcgga tgggatcgta
    10801 gcgggttccg tgacagtccg aggaccccgt ggccacgagc ccgtaacgag ccgacagctc
    10861 ccggtagcga gcctcggtgg cacggtcctg gtcggggtgg gcacactcga tgccgtccat
    10921 ccccgcctcg atcatctccc ggatgagcgg gtgcgggacc gggacgccgt cccgccagtg
    10981 cacggggtgc gcgaggacgg ccaggccgtc cgctcgccgg atcaggcgga ccgcctccac
    11041 gggatggagg gcgtgcttct gcacgtaggc tcggccgccc gtaccgatca ggtctgcggt
    11101 gaacgcgtcc tcgatcgcgg gcaccacgcc cgcctcgacc agcgcctggg ccacgtgtgg
    11161 gcggcccacg ctgcccccgg ccgcgatctc gcgaacccgc tcgacggtga ccggatggcc
    11221 cagtgcctgg agtcgttcca ccatccgctc accgcgccgg atccgatcgt cctgcagcct
    11281 ggcgagctcg gcgcggaact cctcgtgccc ggggtccatc cagtacccga ggacgtggat
    11341 gctcccaccc tcgtactcgg ccgagaactc gacgcccggg acgatctcga gccccgtgcc
    11401 ggccgccgcc tcctcggccg cggagacccc cgccatcgtg tcgtggtcgg tgaccgcgag
    11461 gatgcggagc ccgcgatcga gggccagact caccgactcg cggggctcga acgtcccgtc
    11521 cgaatgacgc gtgtgcgtgt gcaggtcgat gctcatccgc tcgacaagtg tagtgggccg
    11581 tcccccaggt tcatcctccc cgggcctggg tacaacccat gaggactggg gaaggaagag
    11641 gtgagcggga tggcaaccac tgcagagcgt ctcagggagc ccgacatgct tcggtcgtac
    11701 ctccacgctc tcgcgaggag cggcgggagc ggcgaccagg atctggggcg cggccgccag
    11761 cggcgggtct gcgctcagtg cggggagcag gcggtcttca gcccggagac cgacggctgg
    11821 taccgctgcg ggcactgcgg gcgctacgcc tgatcactgc cggatcacga cctccaggtc
    11881 gccccgcttg tgatcgacct tcacagcggt gacgccgtcg ctgttcacga aggagagatc
    11941 gaggcgggcc ccggccacac gcatccctcg cagctcgatc ccggtcagcc agtccggcag
    12001 ccgcggacgc tcgatggtca gccgccgccg cgtcgcgtcc gcggacagtc ctccgtatgc
    12061 ccggaccatg agcagcgggg caccggatgc ccacgcctgg ggccggcagg ccaccgggta
    12121 ctcgacgggg accgggacct cgtcgctgga gaacccgcag aacacctcgg ggaacctgga
    12181 gtaggggaag tggcgaccgg ccgccgtgag ctggtccagg acctcccggg cctccgcgtc
    12241 gaacccatac cgcttgaggc cctgggcgat gagggagtta tcgtggggcc agatcgtgcc
    12301 cgtgtgatag ccgatcgggt cgtaggcgga gtgtcgcgag gagagcgtcc tgatccccca
    12361 ccccgagagc atgccgggat ccatcagccg cttcaccagg tggggggcga ggcgccggtc
    12421 cacgatccgc gaccagagac cgtggccggg attcgacgtg atggtcggca cccggcgctt
    12481 ctccccgtcg agcgcgagcg cgaaggaccc cgtctcctcc acccagaagt cccggttgaa
    12541 gcgccccttg agctcagcgg cctcgcgctc gagccgcgcc gcgaggtcct cgtcgcccat
    12601 ctcccgcgcc agcggcgcca tcaggctctt ggcctgatac acgtatccct ggacctcgac
    12661 cagggcgatg ggcggctcca ccggtgtgcc gtccggccag gggacgccgt cccaagagtc
    12721 cttccatccc tggttgtcga gacccctggg agagcgccgt tcgtactcga cgtagccgtc
    12781 gccgtcccga tccccgtgct cgtcgatcca gcggagtgca gcgagcgcgt tcggccaaag
    12841 ctcctgaagc gcctcccgat cgcccgtcca ccggaacgcc gcgcccagga cgacgagcca
    12901 caagggcgtc gcgtcgatcg atccgtagta gggggtgtgg gggatctcgt cggctccggc
    12961 gagctcaccg acgcgcagct cgtggaggat cttccccggc tcctcctccc gccagggatc
    13021 gtcgatcttc ccctgaagcg ccgcgagccc gcgaagcgtg ctccacgcca gctggggaca
    13081 gcccgtgagc gcctggtagg ccgtgatgag ggagtcccgg ccgaacatcg ccgagtacca
    13141 ggggatcccg gcgtcgatga agcgggtgcc gttctcgccc gtcgaggtga gcatccggag
    13201 gtcccggacg gccctgcgga ggaagccctt gatgcgcccg tcggtggcgt cgaagcgggt
    13261 ggcgcgcttc tcccaggctg cgtactcctg ctccagctcc tgctcgatct cgcgcatcga
    13321 tcggcgcgta ggggccgcgt cgcccacgtg gggctcgatc tccagcacga tgtcgacctg
    13381 ctcgccggcc gccaccgcga ggtcgaacga ggcttcgcgc tcgctcagcg tctcgggggc
    13441 cggcgagaag cggagggtcg tcgatcgctc cacgccgtcc aggccccggt acccgaggat
    13501 cacgctgtga cggtccacct ccggcggcag catctggccc cgctcctccc gggccagccc
    13561 acgcacctcg aagatgtcga ggaagtccga ctcgaaggag acggtcagcc cgatgcggcg
    13621 atcctggagg ccgaagttgc ggatccgcat ccgctcgaag aggctccggt ggaggacccg
    13681 cctgcgctgg agcgagatgt tgtccttccg ctctggaccc gccaggaaga cgtgccgcac
    13741 cgggaagacg agctccacga tctgggagta gttccgctcg gtcgaggagt gcagcagcag
    13801 cgggcgcagc tgattcagcg tgacctcgag ccgcgagagg aaccgcgtgt cggcgtgata
    13861 gaggccgagc gccgcggtgt tgccctccgg gacgtcgccg aagcggttcg agacgaagaa
    13921 gctccggtcg tgcttgatga cctggacctc ccggatgtcc acggtcgccg gcggtctcgc
    13981 cacgagccca tcctaagccg ggccgtcccc ggtcggggag aatggcggcg gaaggaggcg
    14041 cggatgggat cggtgatggg ggccgaccgc ttctacgacg ccggcgagat cgggtgcggg
    14101 ggccccacgc tgaaggagat cgggcggctc gtggacgggc tgccggcggg cggcaacgtc
    14161 gaggtgcgct cgaccacgca cacggggagg aacagcctgc gcgccttctg ccgtctccgc
    14221 gagctggcga tcgaggccga ggacgcggga ccgaaccgcg accgcatcct gatccgcaag
    14281 acgtgaggag gaccgcatga cgatcgtgat ccacgcgagc cacggagcgg aggacccgga
    14341 gcgcgccacc ctgcccttcg tgctcgggaa caacgcggcg gtggctgggc aggatcccgt
    14401 gatcctgctg accgtcgagg ccgtccatct ggccaccgaa gggggagccg aggggatcca
    14461 gaagcagggg ctgccctcgc tccccgagat catggccgag ttcgtgtcga acgggggccg
    14521 gatctgggcg tgccagtcct gcaccacgcc gcgcggcatc gaggaggggg atctcgccga
    14581 gggggccagg atcgggacgt cgatggaggt cgtggagctc ctcgcggggg gcgcggccag
    14641 cttcgcattc tgacctccgc cggccccctg ttatgctcgg gccgtggccc ggatgaccgt
    14701 cttcggggca ggcgcggccg ggaccgcgct cgcgatccat ctggcccgca agggcgagga
    14761 cgcagtgctc tggggcagcg agcacgacgc cgccgtcctc ggcgatctca ccgagaagcg
    14821 ccgccatcct gcgcttcccg agcggctgcc cgaggagctg cgcatcctcg gccccgagga
    14881 cctcgaggag gccggcgccc acgccgagat cgccgtgctc ggggccaact cggccggcgc
    14941 cgggccactc ggccgcctcg tcgcccccgc cctggagggc gcccggctcg cggtctcgat
    15001 cgccaagggg ctgcagcccg ggacgctgga gcgcgtcagc gtcgtctacg cgcgcgagct
    15061 gcccacgatc gcggtcgtgg cgatgagcgg cccctcgctg gcggcggaga tcgccgaggg
    15121 gctgctgacc gccgtggcgt ttgcctgcga ggacgagggg gcatgcaagg aggcggcgga
    15181 ggcgttccgc tcggaccgct tcctcgtgag cacgaccgac gacgtcgtgg gcgtggag
//
LOCUS       DASUTW010000159         6634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_308870, whole genome shotgun sequence.
ACCESSION   DASUTW010000159 DASUTW010000000
VERSION     DASUTW010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6634
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308870"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..851)
                     /locus_tag="VF058_05385"
     CDS             complement(39..851)
                     /locus_tag="VF058_05385"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012692311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX6207776.1"
                     /translation="MRKLIPMLSVVLVLALASPGGAIVNGELDGDDHPNVGALIGDFD
                     GDKIIICSGTLIAPDVFLTASHCTAFLESIGQSTAWVSFDGELEEPVESPPATLHQGT
                     LHTHPDFGFSGPGGASEPHDIAVVVLDQPVQGIQPASLPTLNQLGQAAAKNGLKRQKF
                     TAVGYGVHEAVLGGGPPSFPFDGDRWRAVSEYRSLENAWLHLSQNRSTGDGGTCFGDS
                     GGPNFLGAGAGETDVVAGITVMGDSMCRATNVTYRLDTASARDFLDGFVSLP"
     gene            complement(905..2542)
                     /locus_tag="VF058_05390"
     CDS             complement(905..2542)
                     /locus_tag="VF058_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6207777.1"
                     /translation="MDGPRHGGHLLAALLKREGVEHLFTLSGGHIAPIYDGCVAEGIR
                     IVDFRHEQAAAHAADGWARVTGRPGVAAVTAGPGVTDAVTGVANAFYADSPMLLIGGR
                     APLAEQRMGSLQEMDHVALMRPITKWADTCWQTRRIADYAAEALRHATTGRQGPVFLD
                     VPLDVQFDVWPKDVDLPRDFRPEGRPQGDPDLVKRAAKMLADAERPVVFAGSGVRWSE
                     AHEALAALADALKVPVFLNSLGRGSLPPDHPSFFSAARKHALGKADLVLALGVDWDFR
                     LGFGRRNLSEDVRVIQVDVDGRHVGRNRPVEVGIVGDPGRVIEQLLAEGVGSPREPSW
                     TEEVRDVERAKHEEARAGMESDAVPIHPQRFAREIRDFLDEDATVVGDGGDIVGISSS
                     IVQARRPGHWLDPGPFGCLGVGPSFAIAAKLARPDTQVAVIYGDGSFGLNAMEYESAI
                     RQGIPFVGIIGNDGAWGQIKVAQEALYGEGNAPAATLSTEAGYERMVDGLGGHGERVT
                     DPSELRPALQRAFDSGVAACLNVIVDPSLMRRASYLG"
     gene            2594..3460
                     /locus_tag="VF058_05395"
     CDS             2594..3460
                     /locus_tag="VF058_05395"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6207778.1"
                     /translation="MFRRRRPEAAGTTPQRTPSGYHQEDWESYDHVAEAYERVTAERT
                     SGAARDLVELAGVGPGHQVLDVGTGTGVAAIAAIEHVGAEGLVVGLDPSSKMLALARP
                     KHSEVHYVQGEALDLPFRDASFDRVIGNFVVLHFNRWETALFDMVRALRPGGVMGLSS
                     WAASEDAFTEAWRQVAEELIGPELYRDAARRAAPWQERFSNPALFEESLKKAGLRRIR
                     TERRSYRFEMTLGSYLEGMETRTLARSLHRIMGDAIWERFRERVRDEFDDRFAERIGD
                     TRDVWFGLGVRE"
     gene            complement(3462..4940)
                     /locus_tag="VF058_05400"
     CDS             complement(3462..4940)
                     /locus_tag="VF058_05400"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX6207779.1"
                     /translation="MPRIDYATTPPAKHDADLLVLPVFEGPEEGPGLKEAGRAMGEDL
                     LELLRSNGVRGKRGDAFSVPTFGRLPAGTLLLVGLGKRDEVDADRVRRVIGKLAPRLH
                     GYRSVATSLPQAARGGWEEAVQAAAEGFVLGGYRFLRYKKDEDERKLRRLTLLGTGRW
                     DEGRAKRAVGRGETVAEAACWARDLVNTPAIDATPEFLAAEARKMARETGLEVKVWTK
                     RRLEEGGFGGILGVGSGSANDPRLIELTYSGGRGKPIALAGKGITFDSGGLSLKDAKQ
                     MEWMKADMSGAAAVMATMRAIAELKPKVNVVAAIPSAENMPGGAAIRPGDVLRHRGGK
                     TTEVLNTDAEGRLILADALAYLAERKPRLIVDAATLTGACMIALGDQLYGVMSNDDDL
                     ARELVAAGGDAGEPGWQLPLWDGYREQVSSTLADVKNIGSRYGGAINAALYLSEFVGD
                     IPWAHLDIAGVAYAQNGGDYGKGATGIPVRTLVRFVLDRAGR"
     gene            5019..5342
                     /locus_tag="VF058_05405"
     CDS             5019..5342
                     /locus_tag="VF058_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6207780.1"
                     /translation="MEQANLTDHVHFEESEVARSALFESERLFSQLLCLSRNQSYGPA
                     SDPDSDAMLTVLAGEAAFQVGKRRARLKQWGAVLIPAGADVTVKNASADPLVVLMVTA
                     PPPAG"
     gene            complement(5272..>6634)
                     /locus_tag="VF058_05410"
     CDS             complement(5272..>6634)
                     /locus_tag="VF058_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX6207781.1"
                     /translation="AHADRDRYLTARGTQGAGALTLSFFASALGTWILFAPPEVGTFG
                     GLLGILGYAVGQAAAIAIFAWLGPLVRDRLPSGSTILGWVRGRFGPAASAVVVFVSVG
                     YMFIFLTAELTAIGGVLGLLAGVDPLVPVLAVALATAAYTAYGGLPASVATDRWQGWL
                     VLAFLAIAAGAVVVEIGSPVERLRAGGAGTVTRLGAETFVTLFIAIVAANLFHQGFWQ
                     RVWAARDRDGLVRGAIGGAALILPVVLATGLLGAMAAGAGRAETPSLAFFALLEGLPE
                     PVLWIAVALAVALVASTTDTLQNGLASLAATEVGGRGISLGAARWLTVALTVPGAAIA
                     VQGYSVLRLFLIADLLAAAIAAPVFLGLWRRATSTGVLAGAAAGIVGVVAAGWIATGD
                     LLEGLRLLTLPPDRLDLRLGAFAAAAAASTAVALLVAVLSPRAEAPSPSAPRAGPPTR
                     SSR"
BASE COUNT          956 a         2503 c         2310 g          865 t
ORIGIN      
        1 cgcgggtcgt gtgaacgacc cgcgaccgcg ccccaccctc acggcagcga gacgaagccg
       61 tccaggaagt cccgcgccga ggccgtgtcg agccggtagg tcacgttcgt ggcccggcac
      121 atcgagtcgc ccatcacggt gatgccggcg acaacgtcgg tctcccctgc cccggccccc
      181 aagaagttcg ggcctcccga gtcgccgaag cacgtgcccc catcgccggt cgagcggttc
      241 tgggacaggt ggagccacgc gttctccagc gagcggtact ccgagaccgc ccgccaccgg
      301 tctccgtcga acgggaagga cggcggtccg ccgccgagaa ccgcctcgtg gacgccgtag
      361 cccaccgcgg tgaacttctg gcgcttcagg ccgttcttcg cggcggcctg gccgagctgg
      421 ttcagggtcg gcagggaggc tggctggatg ccctgcaccg gctgatcgag cacgaccacg
      481 gcgatgtcgt ggggttcgga ggcgccgccg ggccccgaga acccgaagtc cgggtgcgtg
      541 tgcagcgtcc cctggtgcag ggtggccggc gggctctcca cgggctcctc cagctcgccg
      601 tcgaacgaca cccacgccgt cgactgcccg atcgactcca ggaacgcggt gcagtgcgac
      661 gccgtgagga acacgtcggg ggcgatcagc gtccccgagc agatgatgat cttgtcgccg
      721 tcgaagtcac cgatcagggc tccgacgttc gggtggtcgt ccccatcgag ctcgccgttc
      781 acgatggcgc cccccggcga cgccagggcg aggacgagga cgacggagag catcgggatc
      841 agcttccgca tcggcagacc tcctcagtct cgaggcaggc gcgagcatac cgcggacgga
      901 ggattcatcc caggtaggag gcccggcgca tcaggctcgg gtccacgatc acgttcaggc
      961 acgccgccac gccgctgtcg aacgccctct ggagcgccgg gcggagctcg ctcggatccg
     1021 tcacacgctc gccgtggccg ccaagcccat ccaccatccg ctcgtacccc gcctccgtag
     1081 agagggtcgc cgccggcgcg ttcccctcgc cgtacagcgc ctcctgtgcc actttgatct
     1141 gcccccaggc gccgtcgttc ccgatgatcc ccacgaacgg gatcccctgc cggatcgcgc
     1201 tctcgtactc catcgcgttc agcccgaacg agccgtcgcc gtagatcacg gcgacctgcg
     1261 tgtccggcct cgccagcttc gccgcgatgg cgaacgacgg cccgacgccc aggcacccga
     1321 acggcccagg gtcgagccag tgaccgggcc gcctcgcctg cacgatcgag gagctgatgc
     1381 cgacgatgtc gcccccgtcg cccaccacgg tggcgtcctc gtcgaggaag tcccggatct
     1441 ccctcgcgaa gcgctgcggg tggatcggga ccgcgtccga ctccatcccg gcccgagcct
     1501 cctcgtgctt ggcccgctcg acgtcccgga cctcctcggt ccaggagggc tctcggggcg
     1561 agcccacccc ctcggccagc aactgctcga tcacgcggcc gggatccccg acgatcccca
     1621 cctcgaccgg ccggttgcgt ccgacgtgcc gcccgtcgac gtccacctgg atcacgcgga
     1681 cgtcctcgga caggttcctc cgcccgaacc ccaggcggaa gtcccagtcc acgccgaggg
     1741 cgagcacgag gtcggccttc cccagcgcgt gcttccgcgc cgcggagaag aacgacggat
     1801 gatccggggg cagggaccct cgccccagcg agttcaggaa caccgggacc ttcagggcgt
     1861 cggcgagggc ggccagggcc tcgtgggcct cagaccagcg gaccccggag ccagcgaaca
     1921 cgaccggccg ctccgcgtcc gccagcatct tcgccgcgcg cttcacgagg tccggatcgc
     1981 cctggggcct gccctccggg cggaaatccc gcggcagatc cacgtccttc ggccagacgt
     2041 cgaactgcac gtcgagcggg acgtcgagga agacgggtcc ctgccggccc gtcgtggcgt
     2101 gacgcagggc ctcggcggcg tagtccgcga ttcgccgggt ctgccagcag gtgtcggccc
     2161 acttcgtgat gggccgcatc agggccacgt ggtccatctc ctgaagcgag cccatccgct
     2221 gctcggcgag gggggcgcgg ccgccgatca agagcatcgg cgagtccgcg tagaaggcgt
     2281 tggccacgcc ggtcaccgcg tcggtgacgc cggggcccgc cgtgacggcg gccacgccgg
     2341 ggcgccccgt cacccgggcc catccgtcgg cggcgtgggc cgcggcctgc tcgtggcgga
     2401 agtccacgat gcggatcccc tcggccacgc agccgtcgta gatcggagcg atgtgccctc
     2461 ccgagagggt gaagaggtgc tccactccct cccgcttcag cagcgccgcg agcaggtggc
     2521 cgccgtgacg tgggccgtcc atcgaccgca tcgtagccgg acggcccgac ctcggtgtgg
     2581 tgcaataggc gccatgttcc ggagacgcag gcccgaggcc gcgggaacca cgccccagcg
     2641 cacgccctcc ggctaccacc aggaagactg ggagtcctac gaccacgtgg ccgaggcgta
     2701 cgagcgggtc acggccgagc gcacgtccgg cgcggcccgc gacctcgtcg agctggccgg
     2761 ggtgggaccc ggccatcagg tcctcgacgt gggcaccggc acgggggtgg ccgcgatcgc
     2821 agcgatcgag cacgtggggg ccgagggcct ggtcgtgggg ctcgatccct cctccaagat
     2881 gctggccctg gcccgtccca agcactccga ggtgcactac gtgcagggcg aagccctcga
     2941 cctccccttc cgcgacgcct cgttcgaccg ggtgatcggc aacttcgtgg tcctccactt
     3001 caatcggtgg gagaccgccc tcttcgacat ggttcgggcc ctccgtcccg gaggcgtgat
     3061 gggcctctcg agctgggcgg cgagcgagga cgcgttcacc gaggcctggc gtcaggtggc
     3121 cgaggagctg atcgggcccg agctctaccg ggacgccgcc cgccgcgcgg cgccgtggca
     3181 ggagcgcttc tcgaacccgg ccctcttcga ggagtcgctg aagaaggcgg gcctccggcg
     3241 gatcaggacc gaacggcgct cctaccgctt cgagatgacg ctcgggtcct acctcgaggg
     3301 catggagacg cggacgctgg cgcgctcgct ccaccggatc atgggggacg cgatctggga
     3361 gcggttccgc gagcgggtgc gggacgagtt cgacgatcgc ttcgccgagc ggatcggcga
     3421 cacccgcgac gtctggttcg gcctgggcgt gagggagtag ctcagcggcc ggcccggtcg
     3481 agcacgaacc gcaccagcgt cctcaccggg atgcccgtgg cgcccttccc gtagtcaccg
     3541 ccgttctggg cgtaggccac gccggcgatg tcgaggtgcg cccacgggat gtcgcccacg
     3601 aactccgaga ggtacagcgc ggcgttgatc gcgcccccgt agcggctccc gatgttcttc
     3661 acgtcggcca gggtcgagga cacctgctcc cggtagccgt cccacagcgg aagctgccac
     3721 cccggctccc ccgcatcccc ccccgccgcg acgagctccc gggccaggtc gtcgtcgttg
     3781 ctcatcacgc cgtagagctg gtcgcccagg gcgatcatgc aggcgccggt gagcgtggcc
     3841 gcgtccacga tcaggcgagg cttccgctcg gccaggtagg cgagcgcgtc ggccaggatc
     3901 agccgcccct cggcgtcggt gttgaggacc tcggtcgtct tcccgcctcg gtggcggagg
     3961 acgtcgccgg gacggatcgc ggcgcccccg ggcatgttct cggcgctggg gatcgccgcc
     4021 acgacgttca ccttcggctt cagctcggcg atggcccgca tcgtggccat gaccgcggcg
     4081 gccccggaca tatccgcctt catccactcc atctgcttgg cgtccttcag cgagagcccg
     4141 ccggagtcga acgtgatgcc cttcccggcc agggcgatgg gcttcccccg gcctcccgag
     4201 taggtgagct cgatcaggcg cgggtcgttg gcgctgcccg agcccacgcc gaggatcccg
     4261 ccgaagcccc cctcctccag gcgccgcttg gtccagacct tgacctcgag gccggtctcc
     4321 cgcgccatct tccgagcctc ggcggccagg aactcgggcg tggcgtcgat cgccggcgtg
     4381 ttcacgaggt cccgggccca acaggccgcc tcggccacgg tctcgccgcg ccctacggcg
     4441 cgcttcgcgc ggccctcgtc ccagcggccc gtgccgagga gagtcaggcg ccgcagcttc
     4501 cgctcgtcct cgtccttctt gtaccggagg aaccggtagc cgcccaggac gaacccctcg
     4561 gcggcggcct ggacggcctc ctcccagccg ccccgggcgg cctggggcag gctcgtggcc
     4621 acgctccggt aaccgtggag gcgtggagcg agcttcccga tcacgcgccg gacgcggtcg
     4681 gcgtcgacct cgtcacgctt cccgagcccg accaggagca gggtcccggc gggcaggcgc
     4741 ccgaacgtgg ggaccgagaa cgcgtccccc cgcttccccc ggaccccgtt cgatcgcaga
     4801 agctcgagga ggtcctcgcc catggctcga cccgcctcct tcagccccgg cccctcctcg
     4861 ggcccctcga agacggggag gacgagcagg tcggcgtcgt gcttggcggg cggggtcgtg
     4921 gcgtagtcga tcctcggcaa gggaacctcc tggagtctgc agaggcgcag tctagaacgc
     4981 tttgccgggc gccgtccggg cccgtacgct ggaccgcgat ggagcaggcg aacctcaccg
     5041 accacgtcca cttcgaggag tcggaggtgg cgcggagcgc gctcttcgag tcggagcggc
     5101 tcttcagtca gctgctctgt ctctcgcgga accagtcgta cgggcccgcg agcgacccgg
     5161 actccgacgc gatgctcacc gtgctcgcgg gcgaggccgc gttccaggtc gggaagcgcc
     5221 gggcgcgcct gaagcagtgg ggagccgtcc tgatcccggc cggggccgac gtcaccgtga
     5281 agaacgcgtc ggcggacccg ctcgtggtgc tgatggtgac ggcgcctccg cccgcgggct
     5341 gaggaccgcg acgagcaggg ccacggccgt cgaggcggcg gcggcggccg cgaaggcccc
     5401 caggcggagg tccagccggt cgggggggag ggtgaggagc cggagcccct ccaggaggtc
     5461 gccggtcgcg atccagcccg cggccaccac gcccacgatg cccgcggccg cgccggccag
     5521 aaccccggtc gaggtcgccc tccgccacag gccgaggaag acgggggcgg cgatcgccgc
     5581 ggccaggagg tcggcgatca ggaacagacg cagcacgctg tagccctgca cggcgatcgc
     5641 cgcccccggc accgtcagcg ccaccgtgag ccacctcgcg gccccgaggg agatgcccct
     5701 gccgcccacc tcggtcgccg ccagcgacgc cagcccgttc tgcagcgtgt cggtcgtgga
     5761 ggccacgagc gcgaccgcga gcgccaccgc gatccagagc accggctcgg ggagcccctc
     5821 cagcagcgcg aagaacgcaa gcgagggcgt ctcggcccgc ccggcccccg ccgccatcgc
     5881 ccccaggagg ccggtcgcca gcaccaccgg gaggatcagc gcggcgcccc cgatcgcgcc
     5941 tcgaacgagc ccatctcggt cgcgcgcggc ccacaccctc tgccagaacc cctggtggaa
     6001 gaggttcgcg gccacgatgg cgatgaagag ggtgacgaag gtctcggccc ccagccgcgt
     6061 caccgtcccc gcgccgccgg cgcggagccg ctcgaccggc gacccgatct cgaccaccac
     6121 cgccccggcc gcgatcgcca ggaacgccag cacgagccat ccctgccacc ggtcggtggc
     6181 caccgaggcc gggagccccc cgtaggccgt gtaggccgcc gtggccaggg ccaccgcgag
     6241 cacgggcacg agggggtcca cacccgccag gagacccaac acgccgccga tcgccgtgag
     6301 ctccgcggtg aggaagatga acatgtagcc gaccgagacg aacacgacca ccgcgctcgc
     6361 cgcggggccg aaccgccccc tcacccatcc caggatcgtc gagcccgagg ggagccgatc
     6421 tcgcaccaaa ggccccaacc acgcgaagat cgcgatggcg gcggcctgcc ccaccgcgta
     6481 gcccaggatc ccgaggaggc ctccgaaggt ccccacctcg ggcggggcga agaggatcca
     6541 cgtgcccagc gccgaggcga agaagctgag cgtgagagcg ccggcgccct gtgtcccgcg
     6601 agcggtgagg tagcggtcgc gatcggcgtg ggcg
//
LOCUS       DASUTW010000160         4868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_309081, whole genome shotgun sequence.
ACCESSION   DASUTW010000160 DASUTW010000000
VERSION     DASUTW010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309081"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1834
                     /locus_tag="VF058_05415"
     CDS             221..1834
                     /locus_tag="VF058_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207782.1"
                     /translation="MQRIRDPRAGRSGGRTVRLEGDDASELERHLAEYTEIIDERLED
                     GLAAIERSAAKLMRHLAAEVWRASGPDARKNLKEGVIAALTQDDAIRGLLSHTDERFQ
                     SLQIRVTRMEEAVRQLAEATRDAVKRVSERAREAEASGVAEEIARQRHDLARFSRRMS
                     EGMGEMYRRIQTGLRSELADVREGIRGELQRLEAAVSSETAFSADAARELREELRALV
                     DGTAERTGVVLEGIHADLSRRLAESMERRARDAESLAERTEALRRDLDERLERLEAAQ
                     GRQSERMSEYAAQAAEGVAEMARRIQGEIAALGERAEEAERRTGERLDGLKGAIEESA
                     RRTSALAERVGRGLTEATARIREGFRMTLESAAEESRRTRETMEEALEREIARLQEEV
                     AGVAARTGEVLEGIQADLARRIEGTAGSMREEAIHLVTQLVGRHAGQQGRSLQAITDS
                     LRALLDAVRKSGQDQTTSIERVLRTSAARQEAALDESLIALRASVEELTGIYASEPRP
                     EDAAVFTRRLRGVERRLERLSRELAGDVA"
     gene            complement(1944..2213)
                     /locus_tag="VF058_05420"
     CDS             complement(1944..2213)
                     /locus_tag="VF058_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207783.1"
                     /translation="MARASMRCDEVVAVLPVFAGEEAPYPHGLEVHLQTCRSCADEEI
                     RYRELLGIVRGLRDPGEPAPPGLTLRVSRRAARLDTAWRGYARRG"
     gene            complement(2188..2748)
                     /locus_tag="VF058_05425"
     CDS             complement(2188..2748)
                     /locus_tag="VF058_05425"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6207784.1"
                     /translation="MEGSKTLVDGELLGACRRGDPAAFEELVARTQHRVYALAYRLVG
                     DRGEAEDVAQEAYLRVFRSLGGFREESRFETWLHRIVVNAAMTHLRRRGRFGDLLRDP
                     ATDPVPPPAPPAQDRAADRDVIRRALEGLPQGMRTTLVLYEMYGLSCREIGREMGLSE
                     GAVKVRLHRARKRLKEAIHGASEHAV"
     gene            complement(2781..3212)
                     /locus_tag="VF058_05430"
     CDS             complement(2781..3212)
                     /locus_tag="VF058_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016578604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX6207785.1"
                     /translation="MTVARRAFEDVAVGDEIPQRTLVVDRAQLVQYAGASGDFNPIHW
                     NPDFARMVGLPGVIAHGMFTMALVARAVGEWAGDPGAVKRISVQFRREVLPEERVVAK
                     GRVVEKDEGSRTVRLELWAELERDGKVLQPIKNGEAVLELS"
     gene            complement(3209..3658)
                     /locus_tag="VF058_05435"
     CDS             complement(3209..3658)
                     /locus_tag="VF058_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017593331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6207786.1"
                     /translation="MALNRELVEKEYPGVTYEVGREKIREFAQAVGETNPIYHDPEAA
                     RAAGHAEQVAPLTFPTVLHLRSGPQVVMDPELGLDYSLVVHGTQEFEYRRPILAGDVL
                     TATPRIAEITAKGPHEFLVIENTMTDPSGEVVCIARATLISRGTAER"
     gene            complement(3662..4468)
                     /locus_tag="VF058_05440"
     CDS             complement(3662..4468)
                     /locus_tag="VF058_05440"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX6207787.1"
                     /translation="MGRLDDRVAIITGAGRGIGRATAVRFAAEGAAVVVNDVDAEPAE
                     ETAQAVKEAGGEALVSTHDTVDLEQAKAMVEGAMERFGKLDVVVNNAGITRDRTFHNL
                     DDELWDLVLDVNLKTAFHTTLAAMPHLRGAAKREMGEQGKPGHHRKITFTTSVAALVG
                     NAGQANYTAAKGGIAALTKTLARELGPFQINVNAVAPGFIETRLTQAKQEGEDLGVPE
                     QIRQMALMLIALGRYGQPEDVANAHLFLASSEADYISGVILPVAGGQLGA"
     gene            4596..>4868
                     /locus_tag="VF058_05445"
     CDS             4596..>4868
                     /locus_tag="VF058_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6207788.1"
                     /translation="MASRGRREEQRIRILIADDHRSYAEALKTVIDLERGLMVTEVVT
                     DGRSAVDRVAVGRPDVVLMDVEMPVMDGIAATREIKRVFPEARIVVL"
BASE COUNT          697 a         1770 c         1745 g          656 t
ORIGIN      
        1 ccggagggac tcgaaccccc aaccgccggt tttggagacc ggtgctctgc cagattgagc
       61 tacggccctc ggcaccggga gtatatcggt aacgcgggaa cctcagtata cgggctcctc
      121 gcggagagcg gcaaaggatc cggggggcgc gggtgcgtga ccccctcgtg ggcctgtcct
      181 acactggcag ggcctcgcgc ggcccagaac gcgaggatac atgcagcgca tccgcgatcc
      241 gcgggccggg aggtccggcg ggcgcaccgt ccggctcgag ggcgacgacg ccagcgagct
      301 ggagcgccat ctcgccgagt acacggagat catcgacgag cgcctcgagg acggcctcgc
      361 cgcgatcgag cgctcggcgg ccaagctgat gcgccacctc gccgccgagg tctggagggc
      421 cagcggcccc gacgcgcgca agaacctcaa ggagggcgtg atcgcggccc tgacccagga
      481 cgacgcgatc cggggcctcc tgagccacac ggacgagcgc ttccagtccc tacagatccg
      541 tgtcacccgc atggaggagg cggtgcgcca gctcgccgag gccacccgcg acgcggtgaa
      601 gcgggtctcg gagcgagcgc gggaggcgga ggcctcggga gtggccgagg agatcgcccg
      661 acagcgacat gacctggccc gcttctcgcg gcggatgagc gagggcatgg gcgagatgta
      721 ccgacggatc cagaccgggc tccggagcga gctggcggac gtccgcgagg gcatccgagg
      781 cgagctgcaa cgcctcgagg ccgcggtgtc ctccgagacc gccttctcgg ccgacgcggc
      841 gcgcgagctc cgggaggagc tgcgggcgct cgtggacggc acggccgagc gcaccggggt
      901 cgtcctcgag gggatccacg cggacctctc ccgccggctg gcggagtcga tggagaggcg
      961 ggcacgcgac gccgagtcgc tggccgagcg gaccgaggct ctccggcgcg atctcgacga
     1021 gcgcctggag cgactcgagg ccgcgcaggg tcgccagagc gagcggatga gcgagtacgc
     1081 ggcgcaggcc gcggagggcg tagccgagat ggcccgccgg atccagggcg agatcgcggc
     1141 cctcggcgag cgagccgagg aggccgagcg ccgcaccggc gagcggctgg acgggctgaa
     1201 gggcgcgatc gaggagtcgg cgcggcggac gtcggcgctg gccgagcgcg tgggcagggg
     1261 gctgaccgag gcgaccgctc ggatccggga gggcttccgg atgacgctgg agagcgcggc
     1321 cgaggagtcg aggcggacca gggagacgat ggaggaggcc ctggagcggg agatcgcgag
     1381 actccaggag gaggtagccg gggtcgcggc ccggacgggc gaggtgctcg aggggatcca
     1441 ggccgacctg gcccgccgca tcgaggggac ggccgggtcg atgcgggagg aggcgatcca
     1501 cctcgtgacg cagctcgtgg ggcgccacgc cggccagcag ggtcgcagcc tccaggcgat
     1561 caccgactcg ctccgggccc tgctcgacgc ggtccgcaag tcgggccagg atcagacgac
     1621 gtcgatcgag agggtcctgc gcacctcggc cgcgagacag gaggccgcgc tggacgagtc
     1681 gctcatcgcg ctccgcgcct ccgtcgagga gctgaccggg atctacgcga gcgagccccg
     1741 acccgaggac gccgccgtct tcacgcggcg cctgcgcggc gtcgagcgcc gcctcgagcg
     1801 tctctcccgg gagctcgccg gcgacgtcgc ctaggacgcc gccgcgcgac ggggccctcg
     1861 acgcaggatg agggcgatcg cggtggcgcc gacgacggcc ccgccgagcc ccgccgccgc
     1921 gtagcgggag cgagggctgc gcgtcagccc cggcgcgcgt agccgcgcca ggccgtgtcg
     1981 agtcgagccg cgcgccggct gacgcgcagc gtcagccccg gcggggcggg ctccccgggg
     2041 tcccggagcc cccggacgat cccgagcagc tcccgatacc ggatctcctc gtccgcgcag
     2101 ctccggcacg tctggagatg cacctcgaga ccgtggggat agggcgcctc ctcccccgcg
     2161 aagaccggga ggaccgccac cacctcgtca caccgcatgc tcgctcgcgc catggatcgc
     2221 ttccttcagc cgcttcctcg ccctgtggag ccgcaccttc accgcccctt ccgagagccc
     2281 catctcccgg ccgatctccc ggcagctcag cccgtacatc tcgtagagga cgagcgtcgt
     2341 cctcatcccc tgtggtagcc cctcgagggc ccgacggatc acatcgcggt cggccgcccg
     2401 gtcctgcgcc ggcggggccg ggggcggcac cggatcggtc gccggatcgc gcaggaggtc
     2461 gccgaaccgg ccccgccggc gcaggtgggt catcgccgcg ttgacgacga tccggtgcag
     2521 ccaggtctca aacctcgact cctcccggaa gccgccgagc gaccggaaca cccgcaggta
     2581 cgcctcttgg gccacgtcct cggcctcgcc tcggtctccc accagccggt aggccagcgc
     2641 gtacacgcga tgctgcgtcc gcgccacgag ctcctcgaac gccgccgggt caccgcggcg
     2701 gcacgcgccc agcagctccc cgtcgaccag cgtcttggac ccctccatcc cccggcaggt
     2761 taccgcgccc ggggatccgt tcaggacagc tcgaggaccg cctcgccgtt cttgatcggc
     2821 tgaagcacct tcccgtctcg ctccagctcc gcccacagct ccagccggac cgtgcgggac
     2881 ccctcgtcct tctccacgac cctccccttg gccacgaccc tctcctcggg gaggacctcc
     2941 cttcggaact gcacggagat acgcttcacg gcgccggggt cgccggccca ctccccgacg
     3001 gcccgcgcca cgagcgccat cgtgaacatg ccgtgggcga tcacccctgg gaggccgacc
     3061 atcctggcga agtccgggtt ccagtggatc gggttgaagt cgccggaggc gcccgcgtac
     3121 tggacgagct gggctcggtc cacgaccagg gtccgctggg ggatctcgtc gcccaccgcc
     3181 acgtcctcga aggccctgcg cgccacggtc atcgctcagc ggtcccccgg gagatgagcg
     3241 tggcccgagc gatgcagacc acctcccccg acgggtcggt catcgtgttc tcgatcacca
     3301 ggaactcgtg gggtcccttc gcggtgatct cggcgatccg aggggtcgcc gtcagcacgt
     3361 cgccggccag gatcggtcgt cggtactcga actcctgggt cccgtgcacg accagcgagt
     3421 agtccagccc cagctccggg tccatcacca cctgcgggcc ggaccggaga tgcagcacgg
     3481 ttgggaaggt cagcggggcc acctgctcgg catgccccgc ggccctcgcc gcctcggggt
     3541 cgtggtagat cgggttcgtc tcgccgaccg cctgggcgaa ctcgcggatc ttctcccggc
     3601 cgacctcgta ggtcaccccc ggatactcct tctccacgag ctcgcggttg agcgccacgc
     3661 gtcaggcccc cagctgaccg cctgccacgg ggaggatcac gccggagatg tagtccgcct
     3721 ccgagctggc gaggaacagg tgcgcgttgg ccacgtcctc cggctgcccg taccgaccca
     3781 gcgcgatcag catcagggcc atctgccgga tctgctcggg gacgcccaga tcctcgcctt
     3841 cctgcttcgc ctgcgtgagg cgcgtctcga tgaaccccgg ggcgaccgcg ttcacgttga
     3901 tctggaaggg tccgagctcg cgggccaggg tcttggtcag cgccgcgatc ccgcccttcg
     3961 ccgcggtgta gttcgcctgg cccgcgttcc ccacgagggc cgccaccgac gtggtgaagg
     4021 tgatcttccg gtgatggccc ggcttcccct gctcccccat ctccctcttc gccgctcccc
     4081 gcaggtgggg catcgccgcc agggtcgtgt ggaaggccgt cttgaggttc acgtcgagca
     4141 cgaggtccca gagctcgtcg tcgaggttgt ggaaggtccg gtccctcgtg atccccgcgt
     4201 tgttcaccac gacgtcgagc ttgccgaagc gctccatcgc gccctcgacc atcgccttcg
     4261 cctgctccag gtccacagtg tcgtgggtgg agacgagggc ctccccgccc gcctccttga
     4321 cggcctgggc ggtctcctcg gccggctccg cgtccacgtc gttcacgacg acggccgcgc
     4381 cctcggcggc gaagcgcacc gcggtggccc ggccgatccc ccgcccggct cccgtgatga
     4441 tggcgaccct gtcgtcgaga cgtcccaacg gttcctcctc gcggcccggt gaaggctacc
     4501 gaagggtagg tacgagggtc aaggcgggga cctgtggaga cgggatccgc cgcggtgctg
     4561 gcaacagcag agcatgggtg cgaggattcc cggtgatggc ctcacgggga cggcgcgagg
     4621 agcagcggat ccgcatcctc atcgccgacg accaccggag ctacgccgag gcgctgaaga
     4681 ccgtcatcga cctcgagcgg ggcctgatgg tgaccgaggt ggtaacggac gggcgctcgg
     4741 cggtggaccg ggtcgcggtg gggcgacccg acgtcgtgct gatggacgtc gagatgcccg
     4801 tgatggacgg catcgccgcg acccgcgaga tcaagcgggt gttccccgag gcccgcatcg
     4861 tggtgctc
//
LOCUS       DASUTW010000161        13489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1733862, whole genome shotgun sequence.
ACCESSION   DASUTW010000161 DASUTW010000000
VERSION     DASUTW010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13489
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733862"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VF058_05450"
     CDS             <1..646
                     /locus_tag="VF058_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207789.1"
                     /translation="GVVVAESGVTSRAQGAEAELAGADAILVGTALMRAPDPAAKLRE
                     LIARPLVKVCGLTREEDVAAAAEAGADMAGFVLAESPRRVERPLDVPGDMLSVGVVVG
                     ASRPIGTDLVQAYASEEGHRGRDGLLLRRGRVVARVPDLPWRGDDPAHLDRAVRMPGR
                     VMLAGGLTPGNVSAAVRRVRPWAVDASRGLESAPGIKDPAKIRAFVAGALEAAS"
     gene            646..1758
                     /gene="trpB"
                     /locus_tag="VF058_05455"
     CDS             646..1758
                     /gene="trpB"
                     /locus_tag="VF058_05455"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX6207790.1"
                     /translation="MRVEIGRGFGGRFVPETLIPALDALSEAWEEARADPGFRREVDR
                     LGRAYAGRPTPLFRAERLAPGRRLYLKREDLLHTGAHKLNNALGQGLLARRLGKRRVV
                     AETGAGQHGVAAAAVCAMLGLDCVVYMGQEDVRRQHPNVQRMRLLGAEVRPVSFGTRT
                     LREAVSEAIRDWIEHVDDTHYMIGSCVGPEPYPDLVATLQEVIGREAREQVLAAEGRL
                     PDAVVACVGGGSNAIGLFRAFVDDPGVRLIGVEAEGAASLSRGRPAVLHGARSYALAD
                     EDGQIHDARSISAGLDYPGVGPEHARLRESGRVEYVTASDEEALEAFRLLSRAEGIVP
                     ALEPAHAIARALELDAELVVVGLSGRGDKDLGTVLG"
     gene            1755..2492
                     /gene="trpA"
                     /locus_tag="VF058_05460"
     CDS             1755..2492
                     /gene="trpA"
                     /locus_tag="VF058_05460"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX6207791.1"
                     /translation="MSTLAIYLMASRGLPGLVEAAVEAGADLVEVGFPHSDPLADGPV
                     IRRSGERALAEGMGPGACLEALARARRRVDVPLVPMTYAAVLEAYGWDRFAADAREAG
                     ATSLIVADLPSDAMPELRRVHLVAPTTPTTRIRLAARRTDGWLYLVSVTGTTGPRSEA
                     PPLLPDLIRRARAATALPLLAGFGIASPAHAAAAARLADGVVVGSRAIEAVEEGGPEG
                     LRDLVAALRAALDAAASGPGLSVGSQP"
     gene            2673..3278
                     /locus_tag="VF058_05465"
     CDS             2673..3278
                     /locus_tag="VF058_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TasA family protein"
                     /protein_id="HEX6207792.1"
                     /translation="MTDAHASLAGGRRERRTGAKIGLTMLILALLGAVVGFGTFSAFS
                     ATTENPGNAFDAGTVSITDDDSGTAMLSLANAKPGDSDAACIVVTYMGTLPATVRLYG
                     TTGGTGLDQYLDLTVTRGTLVGASFDSCAGFTPDAGGGVVYSGTMDGYPDAYGAALVD
                     PDGSWTSGESRAYRFEVSVQDDSGAQGLNASQSFTWEARNT"
     gene            3278..3793
                     /locus_tag="VF058_05470"
     CDS             3278..3793
                     /locus_tag="VF058_05470"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX6207793.1"
                     /translation="MDATLQARASARPRTPVASLAVLGTLTILLFVMVAMMVAGFRFL
                     VVRSGSMEPAIGTGDVVVVETVRPGEAAPGLVVTFRDATRAGSLVTHRVVSVERHGGR
                     YDFVTRGDANTGEERWSVRAEGAIGVVRSVIPMVGYPLHWLGSPTTRVVLLVGGSILV
                     GGALLRRVWRG"
     gene            3799..4422
                     /locus_tag="VF058_05475"
     CDS             3799..4422
                     /locus_tag="VF058_05475"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HEX6207794.1"
                     /translation="MRRAPRLLAAAVGLLVAGFLGSTTAAFSAVTENPANTFQAAPSF
                     CGVRTLAAAGDAWVVTNPSGANHGGDAELYVVSRAQDRRTFVAFDLPPVPAGCALTDA
                     RLRLRATSSTGARTILAYRVSSGWTEGGVTWAIQPGTSGPPASSPSGTGWREWPVLQQ
                     VLEMYASGNHGFMLRDQVEGSGGGGFTQVYDSRESGPDGPALVLTFG"
     gene            4455..5246
                     /locus_tag="VF058_05480"
     CDS             4455..5246
                     /locus_tag="VF058_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6207795.1"
                     /translation="MTLIRTQPGRIWRITLARPDHRNAVNAAMLGELIRALGDAAADR
                     DARVVVLSGEGRHFCAGADIDELARATEGPDGIDYGRALEEVMRSIEHLPVPALAVVQ
                     GSALGAGCQLVVACDLAIAATDATFGIPSGRLGVVVGYENIQRLVLAVGPKRAGALLL
                     GTRTISGQEAADWGLVGEAVPPEDLPDRALLYAEEIADAAPLSVQGSKRGIRQVLSRL
                     SIDREAEGFRVADFDMMAAHAFGSEDLQEGIAAFRERRDPEFRGR"
     gene            5246..5920
                     /gene="nth"
                     /locus_tag="VF058_05485"
     CDS             5246..5920
                     /gene="nth"
                     /locus_tag="VF058_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX6207796.1"
                     /translation="MPRALTRESPLAERGPEVIDLLKREYRGARVALAFETPLECLVA
                     TILSAQSTDKKINEVTATLFQKYRTAEDYLKVPEDELKEDLRPTGFFNQKTKAVRGAC
                     RMIVEEFGSEVPDSMADLVRLPGVARKTANIVLWNAYPERAKEDPDVGIAVDTHVKRV
                     SQRLGLTAQKDPDKIERDLMGLLPRDDWYGFSYVLIEHGRRICDAKRPRCEECVVSHL
                     CPASRV"
     gene            complement(5917..6531)
                     /locus_tag="VF058_05490"
     CDS             complement(5917..6531)
                     /locus_tag="VF058_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEX6207797.1"
                     /translation="MNALAQALPMPRSRGARLASQAALAIGGSLLIAALAQVSIRLPF
                     TPVPISGQTLGVLLVGTAFGWLLGGLTLALYLAEIAVGFPFAAEGNAGYEVLTLVTPS
                     GGYIWGFLAAAILMGWLAGRGWDRSIRSSISVMLLGHIVIYLVGIAWLHASPTFAAFL
                     GHEPSIEETFAAGLYPFIVGDVLKLLLAAGALPLAWRLVGKRGG"
     gene            complement(6566..6724)
                     /locus_tag="VF058_05495"
     CDS             complement(6566..6724)
                     /locus_tag="VF058_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207798.1"
                     /translation="MRPLPEPSHAPIGFSYEVYPTSRRWLGLALLVILTMAVAVAAAV
                     RLVGIADP"
     gene            complement(6730..6987)
                     /locus_tag="VF058_05500"
     CDS             complement(6730..6987)
                     /locus_tag="VF058_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX6207799.1"
                     /translation="MDWVQRARCRDEDPELFFPIGTSGPALEQIERARAVCMQCEVRE
                     ECLEWALASGQDAGVWGGTSEEERKAMRRTRRLGPRVPVLR"
     gene            7135..7896
                     /locus_tag="VF058_05505"
     CDS             7135..7896
                     /locus_tag="VF058_05505"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX6207800.1"
                     /translation="MEPRRPLVIAHRGASADHPENTLTAFAAAAEAGADLVELDARLT
                     ADGAVVVLHDAELERTTDARGLVHRMRLAEVKQADASGGRAPREEIPTLREVLELLEG
                     TGTGVDVEIKNLPGEPSYRPGGEEIVEAVAEALKGFPGAALITSFDAPTLRSAMEAAP
                     DVPTGLLALPGDELIALDLADEDGHRWLLPYAGIFDHDGDDLLDAAARRGIEVGTWTV
                     DDEGRMERLFARGLAAVATNRPALAVAARRRALRP"
     gene            complement(7900..8811)
                     /locus_tag="VF058_05510"
     CDS             complement(7900..8811)
                     /locus_tag="VF058_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX6207801.1"
                     /translation="MRLLLVVNTRAGTVTPRTVRFIERALSDQAKVRIELTRGRGHGA
                     ELASEAAAGDHDVVAVMGGDGTVNEVANGLAGSDVPMAIIPGGGTNVLARSLGIPQEP
                     VRAAGFLLARLSGPGRRIPLGRAAGRYFTFSCGIGLDAEIVRRVERRQRLKRAAGHGY
                     FAWTALRVGLVSYDFKTPRIRARWGPALEHRRDGLTFLISQNVDPYSYLGSLPIKVCP
                     KASLDLRLDAFAGGPVGRIRIVRWALKALGSGRHARDRRALSLHDEGRIEVVAATPHP
                     VQMDGEYLGDHDRLLLESVPGALSILA"
     gene            8863..9231
                     /locus_tag="VF058_05515"
     CDS             8863..9231
                     /locus_tag="VF058_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207802.1"
                     /translation="MRRRRGIPRGFEWVAAEVERAKDALVAAVPASRREPEPLATSIL
                     AFEEALTEAARALEAWEGAPDDLAERCRSAIDEAARRSEWLRLEAPSLDFESLVLVLG
                     DVIAPLDVLGDVEHEVRRRA"
     gene            complement(9228..9743)
                     /locus_tag="VF058_05520"
     CDS             complement(9228..9743)
                     /locus_tag="VF058_05520"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX6207803.1"
                     /translation="MTTIWTVGHSIHPIETFLELLRGAGIRRLVDVRTAPGSRKHPQF
                     GRNALAASLEQAGIGYEWRGEELGGWRKARPDSRHTALRSAGFRGYADHMETEGFRRA
                     LDRLLDEAEGTPTAVMCAEALWWRCHRSMIADAVAARGHEARHVMPDGSIAPHRPRFS
                     RVEDDVPVYDA"
     gene            complement(9740..10519)
                     /locus_tag="VF058_05525"
     CDS             complement(9740..10519)
                     /locus_tag="VF058_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6207804.1"
                     /translation="MLRTVAESTILFRRNITKLVRVPLMVFMSLFQPLLWLILFTQIF
                     QRLGEFPQFQQQGYESYLMFFAPSVLTMTVITSAFQSGMGMVTDLETGMLDKFLISPI
                     RRSSVLLGKVMADGFRMLLQGATVLVIALLMGARIETGIPGAVVMLIVAALFGIVWAG
                     LSNIVALRTRHAELTMMIGILLTFPMLFLSTAMMPEGLLPGWLETVGRFNPVTYVIDT
                     ARAFMNDGYDWGQLGITLVVIAVVGAFTLTGATRAFRRATT"
     gene            complement(10519..11526)
                     /locus_tag="VF058_05530"
     CDS             complement(10519..11526)
                     /locus_tag="VF058_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007976273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6207805.1"
                     /translation="MTKLIEVESLTKVYPDGTRAVDDVSFHVEEGEMFGFLGPNGAGK
                     TTTIRILVTLLPKTSGSARVGGIEVSEDPDTVRRSIGYAAQFIGVDDDLPAHENLVLQ
                     ARLHGIGKAEARRRADELLDVFALSPVADRRAATFSGGMRRRLDLAQALAHRPPLVFL
                     DEPTTGLDPQTRNAMWRYLEELNRGGTTVFLTTQYLEEADRLCTRLAIIDHGRIVVEG
                     SPAELKKEVGGDVVTLALEDDDPETLRRALEAVTAFPGAGEPRVFDHSISLPVKDAGE
                     ALSALVRRLDAAGIPVARLAMSSPTLDEVFLRHTGERMRVEDPNTRAPSSTFRVMRGR
                     R"
     gene            11626..12072
                     /locus_tag="VF058_05535"
     CDS             11626..12072
                     /locus_tag="VF058_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6207806.1"
                     /translation="MKVHELGHLVLYVRDLQRSVRFYRDVLGWEEIGRMGSQGAAFSS
                     GRTHHELLLLEVGEDATPIPPGRRVGMYHFGLKVGTTDDELREAIGALEAGGVQIMGM
                     ADHVVTHSVYIADPDGNEIELYIDVQPERWREDPAAIMSPPRPLRL"
     gene            12115..13437
                     /gene="gabT"
                     /locus_tag="VF058_05540"
     CDS             12115..13437
                     /gene="gabT"
                     /locus_tag="VF058_05540"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEX6207807.1"
                     /translation="MGTINLVTEIPGPRSRELIERKERVVAEPFDLHMPGFIEEGRGA
                     SFTDVDGNTFLDFAGGLGALLTGHAHPKVVEAARAQLERFAHTDFTVVPYESWVELGE
                     RLVTAVGGGDRKAAFFNSGAEAVENAVKIARRATGRSAIMAFEGAFHGRTLMAMTLTS
                     RHAPYKSGFGPFAPEVYRVPFPYAYRSPEPENAGALALEAFERSLTTQVDPDTVAAVI
                     IEPVLGEGGFVPVTPDFLPGLAEICRRHDILLIADEVQTGCGRTGTFLAVEQFGVEPD
                     LITLAKAMGSGYPISAVVGRADVMDAPGPSEIGGTYIGNPVACAAANATLQVIEEEEL
                     VERAQVVGKTIRAAWEEIARDAPEIGDIRGIGAMIGVEMVKDPETKEPDKELVGGVIG
                     EAMQRGLLAVNCGIYKNVLRHLVPLVISDEELDEGLEVLAESVTAARR"
BASE COUNT         1892 a         4778 c         5003 g         1816 t
ORIGIN      
        1 cggcgtcgtg gtggccgagt ctggcgtcac gagccgcgcg cagggcgccg aggccgagct
       61 ggcgggcgcc gacgcgatcc tggtgggcac ggctctgatg cgcgctcccg accccgcggc
      121 caagctgcga gagctcatcg cgcgtcccct ggtcaaggtc tgcgggctca cgcgggagga
      181 ggacgtggcc gccgcggccg aggctggcgc cgacatggcg gggttcgtgc tggccgagag
      241 cccgcgccgc gtggagcggc cgctcgacgt cccgggcgac atgctctcgg tgggcgtcgt
      301 cgtgggagcc agccgtccca tcggcacgga tctcgtgcag gcctatgcgt ccgaggaggg
      361 tcaccgcggc cgggacggcc tcctcctgcg acgtggccgg gtcgtcgccc gagtcccgga
      421 cctgccctgg agaggcgacg acccggccca cctcgaccgc gcggtccgca tgcccggccg
      481 cgtgatgctg gcaggggggc tgacgcccgg gaacgtgagc gccgccgtcc gcagggtgcg
      541 gccctgggcc gtcgatgcca gccgcggcct cgagtccgct ccgggcatca aggatcccgc
      601 gaagatccgt gcgttcgtgg ccggggctct ggaggcggcg tcgtgatgcg cgtcgagatc
      661 ggacgggggt tcggcggtcg cttcgttccc gagacgctga tcccggcgct cgacgcgctg
      721 tctgaggcct gggaggaggc gagggcggac ccggggttcc ggcgggaggt ggaccggctg
      781 ggccgggcgt acgcgggccg gcccacgccc ctgttccggg ccgagcgtct cgccccgggt
      841 cggcgcctct acctgaagcg ggaggacctc ctgcacacgg gggcgcacaa gctgaacaac
      901 gcgctggggc agggactcct cgcccggcgg ctggggaagc ggcgcgtcgt ggcggagacg
      961 ggggcgggcc agcacggtgt ggcggccgcc gccgtctgcg cgatgctcgg cctggactgc
     1021 gtcgtctaca tgggccagga ggacgtgcgg cgccagcacc ccaacgtgca gcggatgcgg
     1081 ctgctcggcg ccgaggtgcg gcccgtctcg ttcgggaccc ggacgctccg cgaggcggtc
     1141 tcggaggcga tccgggactg gatcgagcac gtggacgaca cgcactacat gatcggctcc
     1201 tgcgtcgggc ccgagcccta tcccgacctc gtggccaccc tccaggaggt gatcggcagg
     1261 gaggcgaggg agcaggtcct ggccgccgag ggccgcctgc ccgacgccgt cgtggcctgc
     1321 gtgggagggg ggtcgaacgc gatcggcctc ttccgcgcct tcgtggacga tcccggcgtg
     1381 cggctcatcg gcgtcgaggc cgaaggcgcg gcaagcctct cccggggcag gcccgccgtg
     1441 ctccacggcg cgcgctccta cgcgctcgcc gacgaggacg ggcagatcca cgacgcgcgc
     1501 tcgatctccg cgggcctcga ctacccgggc gtggggcccg agcacgcccg gctgcgcgag
     1561 tcgggccggg tcgagtacgt gacggcctcg gacgaggagg ctctggaggc gttccggctc
     1621 ctgtcgcggg ccgagggcat cgtccccgcg ctggagccgg cgcacgcgat cgcccgggcg
     1681 ctggagctcg acgccgagct cgtcgtcgtc gggctctccg gccgcggcga caaggacctg
     1741 gggacggtgc tggggtgagc acgctcgcga tctacctgat ggcctcacga ggcctcccgg
     1801 gcctggtcga ggcagcggtc gaggccggcg cggatctcgt cgaggtcggc ttcccccact
     1861 cggacccgct cgccgacggc ccggtcatcc gccggtcggg ggagcgcgcg ctggccgagg
     1921 gcatgggccc gggcgcgtgc ctggaagcgt tggcccgggc ccggcggcgg gtggacgttc
     1981 cgctcgtccc gatgacgtac gcggccgtgc tcgaggccta cggctgggac cggttcgcgg
     2041 cggacgcccg ggaggcgggg gcgacgagcc tcatcgtggc cgaccttccc tccgacgcca
     2101 tgcccgagct ccggcgggtg cacctcgtgg cgcccacgac gcccacgacg cggatccgcc
     2161 tcgcggctcg gcgcaccgac ggctggctgt acctggtgag cgtcacgggc accaccggcc
     2221 cccggagcga ggcgccgcct ctcctccccg acctgatcag gcgggccagg gcggcgacgg
     2281 cgctcccgct cctggccggg ttcgggatcg cgtcccccgc ccacgccgcg gccgccgcgc
     2341 ggctcgccga cggcgtggtc gtgggcagca gggcgatcga ggcggtggag gagggcggcc
     2401 cggagggcct gcgcgacctc gtcgccgccc tccgggccgc cctcgacgcg gccgcgtccg
     2461 gaccaggcct gtccgtcggg tcgcagccgt agcggcccgt ggcccgcacg ggcgccgttc
     2521 cgatggcacg gacgcctgcg tgtcccccat ccgtccgaca gggccaccac tcccgagaac
     2581 cctcccgctt cgccatccag cggcctgcta cggctccgat acccgatgtg gaagcgcggg
     2641 ccacgccggt ccgcacgaac gggaggaaga ggatgaccga cgcacacgca tcgctcgccg
     2701 ggggacggcg ggagaggcgc acgggggcca agatcggcct cacgatgctc atcctggcgc
     2761 tcctcggcgc cgtggtgggc ttcgggacct tttcggcctt cagcgcgacg acggagaatc
     2821 ccggcaacgc cttcgatgcg gggaccgtct ccatcaccga cgacgacagc ggaaccgcga
     2881 tgctgtcgct ggccaacgcg aagccgggcg actcggacgc cgcgtgcatc gtggtgacct
     2941 acatgggcac cctgccggcc accgttcggc tgtacggcac caccggcggc accggactcg
     3001 accagtacct cgatctcacc gtgacccggg ggacgctcgt cggggcgtcg ttcgactcgt
     3061 gcgcggggtt cacgcccgac gcgggcggcg gggtggtcta ctcgggcacg atggacggct
     3121 acccggacgc ctacggcgcc gcgctcgtcg accccgacgg gtcgtggacg agcggcgagt
     3181 cgcgcgccta ccgcttcgag gtctccgtgc aggacgacag cggtgcccag gggctgaacg
     3241 ccagccagag cttcacctgg gaggctcgga acacctgatg gacgccacgc tccaggctcg
     3301 ggcgagcgcg aggccccgga cccccgtggc ctccctcgcc gtcctgggga cgctcacgat
     3361 cctcctcttc gtgatggtgg cgatgatggt ggccgggttc cggttcctgg tggtgcggag
     3421 cggaagcatg gagccggcca tcgggaccgg ggacgtggtc gtggtcgaga ccgtcaggcc
     3481 gggggaggct gcccccggcc tggtcgtcac gttccgcgac gccacgaggg ccggcagcct
     3541 ggtgacgcac cgcgtggtga gcgtcgagcg gcacggcggc cgctacgact tcgtcaccag
     3601 gggcgacgcc aacaccggcg aggagcggtg gtcggtgagg gcggagggcg cgatcggggt
     3661 cgtgcgctcc gtgatcccga tggtcgggta tcccctccac tggctgggct ccccgaccac
     3721 gcgggtcgtg ctcctcgtcg ggggctcgat cctggtcggc ggggcgctcc tacggcgcgt
     3781 ctggcgaggg tgagggccgt gcggagggcg ccgcgcctgc tcgccgcggc ggtcgggttg
     3841 ctggtcgcgg ggttcctcgg ctcgacgacc gccgcgttct ccgcggtcac ggagaacccc
     3901 gccaatacct tccaggccgc cccctcattc tgcggcgtcc ggacgctcgc cgccgccggc
     3961 gacgcctggg tcgtcacgaa cccctccggt gccaatcacg gtggggacgc cgagctctac
     4021 gtggtctccc gagcccagga ccggcggacg ttcgtggcat tcgatctgcc tccggtcccg
     4081 gcgggatgcg ccctgaccga cgcgaggctg cggctgcgcg ccacgtcctc gaccggcgcc
     4141 cggacgatcc tcgcgtaccg ggtatcctcc ggctggaccg aagggggcgt gacctgggcg
     4201 atccagccgg gcacgtccgg accgcccgcg tcgagcccct cgggcacggg ctggcgggag
     4261 tggccggtcc tccagcaggt cctcgagatg tatgcctccg ggaaccacgg cttcatgctg
     4321 agggaccagg tcgagggctc aggcggggga ggcttcaccc aggtctacga cagccgggaa
     4381 tcgggccccg acggccccgc cctcgtgctc acgttcggct gaccgaccgc gcggggccgc
     4441 gctagcatga ccgcatgacg ctgatccgaa cccagcccgg gcgcatctgg cggatcacgc
     4501 tggcacgccc ggatcaccgc aacgccgtga acgcggcgat gctgggggag ctgatccggg
     4561 cgctgggcga cgccgctgcc gaccgggacg cccgggtcgt cgtgctctcg ggggaggggc
     4621 ggcacttctg cgcgggcgcc gacatcgacg agctggcccg cgccacggag gggcccgacg
     4681 gcatcgacta cggccgcgcc ctcgaggagg tcatgcgctc gatcgagcat ctccccgtgc
     4741 cggcgctggc ggtcgtgcag gggagcgcgc tcggcgccgg atgccagctc gtcgtggcct
     4801 gcgacctggc gatcgccgcc accgacgcca cgttcggcat cccctcgggg cgcctgggcg
     4861 tggtcgtggg gtacgagaac atccagcggc tcgtgctggc ggtcgggccc aagcgcgccg
     4921 gcgccctgct gctggggacg aggacgatct ccggccagga ggcggccgac tggggactcg
     4981 tgggcgaggc cgtcccgccg gaggatctcc ccgaccgcgc gctcctctac gcggaggaga
     5041 tcgccgacgc cgccccgctg tccgtccagg ggtcgaagcg ggggatccgg caggtgctct
     5101 cccgtctctc gatcgaccgc gaggccgagg ggttccgagt cgccgacttc gacatgatgg
     5161 ccgcgcacgc gttcggcagc gaggacctcc aggaggggat cgccgcgttc cgcgagcggc
     5221 gagaccccga gttccgcggg cgctgatgcc gagggccctc accagggagt cgccgctcgc
     5281 ggaacgcggc cccgaggtga tcgacctcct caagcgggag taccggggcg cccgcgtggc
     5341 cctcgccttc gagacgcccc tggagtgcct ggtggcgacg atcctctccg cgcagtccac
     5401 cgacaagaag atcaacgagg tgaccgcgac cctcttccag aagtaccgga cggccgagga
     5461 ctacctgaag gtccccgagg acgagctgaa ggaggatctc cgccccaccg ggttcttcaa
     5521 ccagaagacg aaggcggtcc gcggggcctg ccggatgatc gtggaggagt tcggaagcga
     5581 ggtccccgac tcgatggccg acctcgtgcg gctgccgggc gtggcgcgca agaccgccaa
     5641 catcgtgctc tggaacgcgt atccggagag ggcgaaggag gaccccgacg tgggcatcgc
     5701 ggtggacacc cacgtgaagc gtgtctccca gcggctgggg ctcaccgcgc agaaagaccc
     5761 cgacaagatc gagcgcgacc tgatgggcct gcttccgaga gacgactggt acggcttcag
     5821 ctatgtgctg atcgagcacg ggcggcggat ctgcgacgcg aagcggcccc ggtgcgagga
     5881 gtgcgtcgtc agccacctct gcccggcgag ccgggtctag ccaccgcgct tccccacgag
     5941 ccgccacgcg agcggcaggg cgcccgccgc cagcagcagc ttgaggacgt cgcccacgat
     6001 gaacgggtag aggcccgcgg cgaacgtctc ctcgatgctc ggctcgtgac cgaggaaggc
     6061 ggcgaacgtg ggtgaggcgt ggagccacgc gatccccacg aggtagatca cgatgtgccc
     6121 gagcagcatc acgctgatcg aggagcggat gctgcggtcc cacccccgcc ccgccagcca
     6181 gcccatcagg atcgcggccg ccaggaaccc ccagatgtac ccgccgctgg gggtgacgag
     6241 ggtcaacacc tcgtaccccg cgttgccctc cgcggcgaac gggaacccca cggcgatctc
     6301 ggccaggtac agcgccaggg tcagcccccc gagcagccag ccgaacgcgg tgccgaccag
     6361 gagcaccccg agggtctgcc cgctgatggg caccggcgtg aacggcagcc gaatcgagac
     6421 ctgggcgagc gccgcgatca gcaggctccc tccgatcgcc agcgcggcct gggacgcgag
     6481 tcgagcgcct cgggagcggg gcatgggcag ggcttgggcg agcgcgttca cattccctcc
     6541 tccttcggtc ggggcggtcc cacggtcacg gatccgcgat cccgacgagt cggacggccg
     6601 cagcgacggc caccgccatt gtcagaatca cgagcagggc cagaccgagc caccgtcgcg
     6661 aggtggggta gacctcgtag gagaagccga tcggcgcgtg gctcggctcg gggagcggcc
     6721 gcacggcggt cagcgcagaa ccggcaccct cggcccgagg cggcgagtgc ggcgcatcgc
     6781 cttccgctcc tcctcggagg tgcctcccca gacgccggcg tcctgaccgg acgccagggc
     6841 ccactcgagg cactcttcac gaacttcgca ctgcatacag acggcgcgag cgcgctcgat
     6901 ctgctcgagg gccgggccgg aggtcccgat ggggaagaac agctcggggt cctcgtccct
     6961 gcacctcgcc cgctgtaccc agtccatccg tccccttccc cggcctggcc cggtctcgtg
     7021 gagcacttca caaggccccc tgaagaagag gccccggccg gcgcgagcgg cagtatagtg
     7081 ttggtctgcg gcccgtgtaa agacccattt ctcgtacgca tcggagcgcc ggacgtggaa
     7141 ccccgccgtc ccttggtgat cgcccaccgc ggtgcctccg ccgatcatcc cgagaacacc
     7201 ctgacggcgt tcgccgcggc ggcggaggcc ggcgccgacc tcgtggagct cgacgcgagg
     7261 ctcacggccg acggcgccgt cgtggtcctg cacgacgccg agctcgagcg gaccaccgac
     7321 gcgcgggggc tcgtgcacag gatgcggctg gccgaggtga agcaggccga cgcctcggga
     7381 gggcgggcac cgcgggagga gatccccacg ctgagagagg tcctcgagct gctcgagggg
     7441 acggggacgg gggtggacgt ggagatcaag aacctcccgg gcgagccctc gtaccggccg
     7501 gggggcgagg agatcgtcga ggcggtcgcg gaggcgctga aggggttccc gggagccgcg
     7561 ctgatcacct cgttcgacgc ccccaccctc cgttcggcca tggaggcggc tccggacgtc
     7621 cccactggcc tgctcgctct gccgggggat gagctgatcg cgctggatct ggccgacgag
     7681 gacggccacc gttggctcct cccctacgcc gggatcttcg atcacgacgg ggacgacctg
     7741 ctcgatgccg cggctcggcg gggcatcgag gtgggcacgt ggaccgtgga cgacgagggg
     7801 cggatggagc gcctgttcgc ccgcgggctc gccgcggtgg ccacgaaccg gccggccctc
     7861 gccgtggccg cgcgccggcg cgcgctccgc ccctgacgct caggccagga tcgacagggc
     7921 cccggggacc gactccagga ggaggcggtc gtggtcgccg aggtactccc cgtccatctg
     7981 gacggggtgc ggagtggccg cgacgacctc gatccgtccc tcgtcgtgga gcgagagggc
     8041 gcggcggtcg cgcgcgtggc gcccgctccc cagcgccttg agggcccagc ggacgatccg
     8101 gatcctgccg accggcccgc cggcgaacgc gtccaggcgg aggtccaggc tcgccttggg
     8161 gcagaccttg atggggaggg agcccaggta gctgtacggg tcgacgttct gggagatgag
     8221 gaacgtgagg ccgtctcgcc ggtgctcgag cgccgggccc cagcgcgcgc ggatcctcgg
     8281 cgtcttgaag tcgtaggaga cgaggccgac ccggagcgcc gtccaggcga agtagccgtg
     8341 gccggcggcc cgcttcaggc gctggcgacg ctccacgcgg cgaacgatct cggcgtcgag
     8401 cccgatgccg cacgagaagg tgaagtagcg ccccgccgcc cggcccaggg ggatgcggcg
     8461 gccgggcccg gacagcctgg ccaggaggaa ccccgccgcg cgcacgggct cctgggggat
     8521 cccgagcgag cgggcgagga cgttcgtgcc ccctcccggg atgatggcca tgggaacgtc
     8581 cgagcccgcg aggccgttcg ccacctcgtt gacggtgccg tcgcctccca tcacggccac
     8641 cacgtcgtga tcccccgccg ccgcctcgct cgccagctcg gccccgtggc cccgcccccg
     8701 cgtcagctcg atccggacct tcgcctgatc ggacagggct cgctcgatga accgcaccgt
     8761 gcggggcgtg acggtgcccg cccgcgtgtt gacgaccagg aggagccgca tcgcgccatt
     8821 ctcacggaag gagggccgag atggaacccg tagactcgcc gcatgaggag gcgacgaggg
     8881 atcccgcgcg ggttcgagtg ggtggccgcg gaggtggagc gagcgaagga cgccctcgtg
     8941 gccgccgtgc ccgcttcccg ccgcgagccc gagcccctgg ccacctcgat cctggccttt
     9001 gaggaggcgc tgaccgaggc tgcccgggcg ctcgaggcct gggagggcgc gccggacgac
     9061 ctggccgagc ggtgccgatc ggcgatcgac gaggccgcgc gccggtccga gtggctcagg
     9121 ctcgaggcgc cgtcgctgga cttcgagtcg ctggtgctcg tgctcggcga cgtgatcgcc
     9181 cccctcgacg tgctcggcga cgtcgagcac gaggtgcgcc ggcgcgccta ggcgtcgtag
     9241 accgggacgt cgtcctcgac ccgggagaac cgtggccggt ggggggcgat ggagccgtcg
     9301 ggcatcacgt gtcgagcctc gtggccccgg gccgccaccg cgtcggcgat catcgagcgg
     9361 tggcaccgcc accagagcgc ctcggcgcac atcacggccg tgggggtccc ctcggcctcg
     9421 tcgagcaggc ggtccagcgc ccggcggaag ccctccgtct ccatgtggtc cgcgtacccg
     9481 cggaacccgg ccgaccgcag ggcggtgtgt cgggagtccg gccgggcctt ccgccagccc
     9541 cccagctcct cgccccgcca ctcgtagccg atccccgcct gctccagcga cgcggccagg
     9601 gcgttccgtc cgaactgcgg gtgcttcctc gacccggggg cggtccgcac gtccacgagc
     9661 cgccggatgc cggccccgcg cagcagctcg aggaaggtct cgatcgggtg gatgctgtgg
     9721 ccgaccgtcc agatcgtcgt cacgtggtgg cgcggcggaa ggcgcgcgtg gcgccggtca
     9781 gcgtgaaggc gccgaccacc gcgatgacga ccagcgtgat cccgagctga ccccaatcgt
     9841 acccgtcgtt catgaacgcg cgggccgtgt cgatcacgta cgtcaccggg ttgaagcgcc
     9901 ccaccgtctc cagccagccg ggcagcagcc cctccggcat catcgccgtg gagaggaaca
     9961 gcatcgggaa ggtcaggagg atgccgatca tcatcgtcag ctccgcgtgt cgtgtccgca
    10021 gggccacgat gttcgacagc ccggcccaga cgatcccgaa cagggcggcc acgatcagca
    10081 tgacgaccgc ccccgggatc ccggtctcga tcctggcccc catgaggagc gcgatgacga
    10141 gcaccgtcgc cccctgcagc agcatgcgga agccgtcggc cataaccttc cccagcagga
    10201 cgctggaccg ccggatcggg ctgatgagga acttgtcgag catccccgtc tcgaggtcgg
    10261 tgaccatccc catgcccgac tggaaggccg aggtgatgac cgtcatcgtc agcacgctcg
    10321 gggcgaagaa catgaggtac gactcgtagc cctgctgctg gaactgcggg aactccccca
    10381 ggcgctggaa gatctgcgtg aacaggatca gccacagcag cggctggaac agcgacatga
    10441 acaccatcag agggacccgg acgagcttgg tgatgttccg cctgaagagg atcgtcgact
    10501 cggccacggt gcgcagcatc agcgcctccc ccgcatgacg cggaacgtcg agctcggggc
    10561 cctcgtattc gggtcctcca cccgcatccg ctcgcccgtg tggcggagga agacctcgtc
    10621 gagggtgggc gaggacatcg ccagcctggc caccgggatg cccgcggcgt ccagccggcg
    10681 cacgagcgcc gacagcgcct cgcccgcgtc cttcaccggc agcgagatcg agtggtcgaa
    10741 cacccgcggc tccccggccc cggggaacgc ggtcacggcc tcgagcgcgc gccgcaacgt
    10801 ctcgggatcg tcgtcctcca gcgcgagcgt gaccacgtcg ccccccacct ccttcttcag
    10861 ctcggccggg gatccctcga ccacgatcct tccgtggtcg atgatggcca gccgcgtgca
    10921 gaggcgatcg gcctcctcca ggtactgggt ggtgaggaac accgtggtcc caccccggtt
    10981 cagctcctcc aggtagcgcc acatcgcgtt gcgcgtctgc ggatcgagcc cggtggtggg
    11041 ctcgtccagg aagacgagcg gcggccggtg cgccagggcc tgggccaggt cgaggcgccg
    11101 acgcatcccc cccgagaagg tcgcggcgcg cctgtcggcg acgggggaga gggcgaacac
    11161 gtcgagcagc tcatcggcgc gcctccgggc ctcggctttc ccgatcccgt ggaggcgggc
    11221 ctggagcacc aggttctcgt gcgcgggcag gtcgtcgtcc accccgatga actgggcggc
    11281 gtacccgatc gaccggcgga cggtgtcggg gtcctcgctc acctcgatcc cgcccacccg
    11341 ggccgagccg gaggtcttgg gaagcagggt gacgaggatg cggatcgtgg tggtcttccc
    11401 ggccccgttc ggccccagga agccgaacat ctctccctcc tcgacgtgga aggagacatc
    11461 gtccacggca cgggtgccgt ccgggtagac cttcgtgagg gactcgacct cgatcagctt
    11521 cgtcatgggc gcctcatcaa gggtacgcgg gcatacgggg aaacgccccg agagggcgag
    11581 gtgttcccag gctatcctgg aggctcacgc gacaaggagg cctcgatgaa ggtgcacgag
    11641 ctcggccatc tcgtcctcta cgtccgggac ctccagcgct cggtccgctt ctatcgcgac
    11701 gtgctggggt gggaggagat cggcagaatg ggcagtcagg gcgctgcctt ctcgtccggc
    11761 cgtacccacc acgagctgct gctgctggag gtgggggagg acgccacgcc gatcccgccc
    11821 ggcaggcgcg tgggcatgta ccacttcggg ctgaaggtgg gcacgaccga cgacgagctg
    11881 cgcgaggcga tcggggcgct ggaggccgga ggcgtccaga tcatgggcat ggcggaccac
    11941 gtggtgacgc acagcgtcta catcgccgac ccggacggga acgagatcga gctgtacatc
    12001 gacgtccagc cggagcgctg gcgggaggat ccggccgcga tcatgagccc gccgcggccg
    12061 ctgcgtctct gatcctctgg tagcctccga gggcgcgaga gggaggaggg cgagttgggc
    12121 acgatcaacc tggtcaccga gatcccgggg ccgaggtccc gcgagctgat cgagcggaag
    12181 gagcgggtcg tcgccgagcc gttcgacctc cacatgcccg gcttcatcga ggagggccgg
    12241 ggggcgtcgt tcacggacgt ggacgggaac accttcctcg acttcgccgg cgggctcggc
    12301 gccctcctca ccggtcacgc ccatcccaag gtcgtcgagg ccgcccgggc ccagctggag
    12361 cgcttcgccc acaccgactt caccgtcgtg ccctacgagt cgtgggtgga gctgggcgag
    12421 cgtctcgtga cggccgtggg cgggggcgac cggaaggccg ccttcttcaa ctcgggggcg
    12481 gaggccgtcg agaacgccgt caagatcgcg agacgggcca cgggccgctc ggcgatcatg
    12541 gccttcgagg gcgccttcca cggtcggacc ctgatggcga tgacgctcac cagccgccac
    12601 gcgccctaca agagcgggtt cgggccgttc gcgcccgagg tgtaccgggt cccgttcccc
    12661 tacgcgtacc ggagccccga gcccgagaac gccggggcgc tggccctcga ggcgttcgag
    12721 cgctcgctga cgacgcaggt tgatccggac accgtcgcgg ccgtgatcat cgagcccgtg
    12781 ctcggggagg gcgggttcgt gcctgtcacg cccgacttcc tgcccggcct ggccgagatc
    12841 tgcaggcgcc acgacatcct cctgatcgcc gatgaggtcc agacgggatg cggacgcacc
    12901 ggcaccttcc ttgccgtgga gcagttcggc gtggagcccg acctgatcac gctggccaag
    12961 gccatgggct cgggctaccc gatctccgcc gtcgtggggc gggccgacgt catggacgcc
    13021 cccggtccct ccgagatcgg cggcacctac atcgggaacc cggtggcatg cgccgcggcg
    13081 aacgccacgc tccaggtgat cgaggaggag gagctggtcg agcgggccca ggtcgtgggg
    13141 aagacgatcc gcgccgcctg ggaggagatc gcgcgagacg cccccgagat cggcgacatc
    13201 cggggcatcg gggcgatgat cggcgtggag atggtgaagg atcccgagac gaaggagccc
    13261 gacaaggagc tggtgggcgg ggtcatcggg gaggcgatgc agcgcggcct cctcgccgtg
    13321 aactgcggga tctacaagaa cgtcctccgc cacctggtgc cgctcgtgat ctccgacgag
    13381 gagctcgacg aggggctgga ggtcctggcc gagtcggtga ccgcggcccg gcgctgagtc
    13441 agcggcgttg ttcgacgcgg cgcacgtcgt ccttcggatc gacgtgcgc
//
LOCUS       DASUTW010000162        12901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_957557, whole genome shotgun sequence.
ACCESSION   DASUTW010000162 DASUTW010000000
VERSION     DASUTW010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12901
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957557"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VF058_05545"
     CDS             <1..805
                     /locus_tag="VF058_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207808.1"
                     /translation="NDGSLVAKAEKEVGMDVMLAETPTPLREGTWSPLLRAAGWAALA
                     TAALIPIQIAVFVAFPYPETTQGWFGLLQGNPLAGLVDLDLLLVVDNVLLVVIALALY
                     VALRPARPGVMLAATGLWLTALVLLIASNPAIEMLSLSGRFAEATTETQRAATLGAGE
                     AVLATWEGTAFQVSYVVGQLAGIAIGLVMLRAGSFGRAIPILMIAGNTIGFGYYLPVV
                     GLAISAFSGVVLWAWYVLLGLRLLRMARSPGGFDEVAVRGRAGRVREGE"
     gene            807..1598
                     /locus_tag="VF058_05550"
     CDS             807..1598
                     /locus_tag="VF058_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX6207809.1"
                     /translation="MRDFARRHEIAAYVMLAYGISWIVWAPAVLTGAGGMALVALGAF
                     GPPIAAGLVIRWTGGSVRGWLRPLLRWRVPGWYWLFALGLPALLFAVVNLVLTLLGEP
                     VELGRLASALPAYIATFVVVALVGGGQEESGWRGFLLDRLQARFSPLAATLLLGVIWG
                     LWHLPIYGIGFVGPMMFVFFYTWLWNRTRSLLLCVVLHGSFTAALDNLFLVNDSLTVD
                     LTILGTLVGGAVSIVLATRGRLGFDQPLVVERREPAPAEVSGVPA"
     gene            1606..3021
                     /locus_tag="VF058_05555"
     CDS             1606..3021
                     /locus_tag="VF058_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX6207810.1"
                     /translation="MMDRLGPQDLMMVWPEELGWSQDIGAIAILDGRGFAEEDGGFPI
                     EAIREHVRGRLHLLPRFRQRLHRPPLGLGWPLWVDAPSIDLRRHVRMLPLEGPADEER
                     LLATCERLRRRRLDRSRPLWELWFLPGLTGGRVGFYMRLHHAVADGVAGIAALGAFVD
                     PVPDPPQTPQPTWRPRPTPTAMQLLADNVRWRLATLGRFFDKLAHPSRTLRAARRGWP
                     AVREAFIEERAPRTSLNRRVGWDRRLDVIRVELDAVKRIAHAHGGKVNDVLLAAVAGG
                     ARALLLGRGERVEGLSLRAFVPVSLHGAETDGAGGNLDGAMMAPLPIGEKDPRRTLRR
                     IAAETAERKRKPRPPGGTLFRNVPIQRAWLRYAPHQRVMNTYVANVPGPPVPLYFAGA
                     PVLEAFPVVPLIGNVTIGVGALSYAGQFNLTVVADRDACPDLERFVVGARRTLDALAR
                     ATGGVVGDGSGTPSTGEGGKP"
     gene            3018..3512
                     /locus_tag="VF058_05560"
     CDS             3018..3512
                     /locus_tag="VF058_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020635433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEX6207811.1"
                     /translation="MKVLVSAASKHGSTAEIARAVADELEGRGQEATVMPPEEVSSVE
                     GYDALILGSAVYAGHWMKPALELVERLGADLKSLPVWLFSSGPIGDPPKPEEDPVDVA
                     DVTEAADARGHRVFPGKLVKKDLGFGEKAIVMALRAPEGDFRDWDEIRGWASEIAEAL
                     EGGR"
     gene            3795..5033
                     /locus_tag="VF058_05565"
     CDS             3795..5033
                     /locus_tag="VF058_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207812.1"
                     /translation="MRRVGILVLAAGVLVGTTSAVAAAKPKTRLVSKTSQGQAGDDTS
                     VEARISNDGRYVVFQSQADNLPGPDDVQGIHVHDRKTKKTRLVSRTSSGQPALGQNRN
                     PDISGNGRFVVFESDASNLPGNGFQVYVRDLKTKKTRLASRNSAGQPADASGLSGRIS
                     DSGRFVTFSSMAGNLPAGGGPLSKLYIHDRKTRKTRLLSKSSNGTPVSAGLHALSASG
                     RFAAFTSSDPALPGPSTFRVYVRDRKTKKTRLVSKRSSGVPFDEFASWVDISGSGRVV
                     VFSLNIEFNEICDRVDIHARDRKTKKTRLVSRTTAGAAADGCNQWPAISGNSRYVAFH
                     SNAPNLPGASPPIARSYVHDRKRRTTRVLSKTTAGTVVTGAGPGLSWTGEWATFVSNE
                     AALPGPNTGVFQVYVRGPLR"
     gene            complement(5030..6133)
                     /locus_tag="VF058_05570"
     CDS             complement(5030..6133)
                     /locus_tag="VF058_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX6207813.1"
                     /translation="MPVLTRRALNRALLGRQLLLERAALSVPRAVERLVGLQAQEPPD
                     PYVALWSRLEGFHHEDLADLLVRRRAVRLTLMRGTIHLVTARDALAMWPVMKPQLERN
                     FHGHLWGRNLTDLDLEELLAAGRKLVEEQPRTAAQLREALAKRWPDHDETSLVPAARY
                     LLPMVQVPPRGVWGASGLPTLTTAERWLGRPLGKSARPDRMILRYLAAFGPASIADMR
                     TWSGLTGLREVVERLRPRLRTFRNEGGRELFDVEDGLLPDPDRDAAARFLPTYDNVTL
                     SHDDRRRIVEDEDRHRMQERMLRGNVGSVLVDGFVRGMWKVSRSGEGATLEIVPLGRL
                     SKPESTAVGEEGERLLTFVAGDAADQDVRIARG"
     gene            6217..7836
                     /locus_tag="VF058_05575"
     CDS             6217..7836
                     /locus_tag="VF058_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEX6207814.1"
                     /translation="MDDRWYKNAIIYEIDLRSYADSDGDGIGDLRGLIDRLDYLAALG
                     ITCIWLLPFSPSPWRDGGYDVTDHYGVHPELGTPGDFVELVHRAAARGIRVIIDLVLN
                     HTSDEHPWFQAARDPDSPLRDHYVWADTPPDDAERGVVFPGVQESTWTLDERSGKHYF
                     HRFYEFQPDLNVANPDVRAEFRRILGYWIQQGVAGFRMDALPFLIEPAGVERPKPEPH
                     FEYLKELRAFLQWRRGDAVLLGEANVERDRVEDYFTTGGMHMIFNFPLNQLVWLALAK
                     GEAGPIARGLEHTAGIPATDQWATFLRNHDEVDLGRLSEKDRQMVFEAFGPEPSMQLY
                     DRGIRRRLAPMLGGDRRRIEMAMSLLFSLPGTPVILYGDEIGMGDLLSLDEREAVRTP
                     MQWNDEENTGFSTADPDRLARPVISGGDYGYERVNVAAQMRDAGSLLSWMKRLVHTRR
                     EAPEVGLGDWELLETGNEHVFGLRAEWEGREVAVYHNLDGEPQKARMGLEPTELSHPL
                     ELLGDRDYDEGWSPFELAGYGYRWARVRGVA"
     gene            complement(7849..9864)
                     /locus_tag="VF058_05580"
     CDS             complement(7849..9864)
                     /locus_tag="VF058_05580"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207815.1"
                     /translation="MRRALLALTVLSLVLVPYRAAGAPGDLHLASITADGVKGEWSTQ
                     PSLSGDGTRVAFSSGSQLHPDDDDAETDAYVKDLTTSEIWLASTSASGEKGNARSSHA
                     AISADGNRVAFLSGATNLHPSGATGLMVKDLTTGEVFLVAPGGEYPALSIDGTRVAFS
                     TISTGLDPRDDDETADIYVRDLVSGELFLASTSSEGIKANGDFFHGSVGAALSGDGIM
                     VAFASGATNLHPGDGDDIFDAYVKNLETGELRLISTAPDGVKGDGNSFSPSLSADGSI
                     AAFRSYSSNLVPGFSGGFGQIFVKDLATGDVRPASLSASGERADRGAADPSLSADGTR
                     VAFSSGATNLHPADADVVSDIFVKDLSTGGVELASTSSSGIKGNGTSYQVSLGDRGAA
                     VAFRSDATNLDPADGESGPDVYVKELGGPEPPSEATGDLSISQAASPDPVPAGEPITY
                     DVRVRNAGPAEATGVAMLDELPDGVTFDSATASQGGGCSESGGIVRCDLGSLAPGQTA
                     DASVVVVPQDLGYLTNTVTVQANEPDPNTLDNRSSINTDVDPAADVAVSLTDSPDPVR
                     VRQPLTYTISIENRGPTWATLELVDTLPSGVRVEAVDAGGSSCTVSRDEIVCRIFGVP
                     DGGSATVRIVVSARKPAALANRATAVPLHFDPDPANNSAEETTVVVR"
     gene            9985..10548
                     /locus_tag="VF058_05585"
     CDS             9985..10548
                     /locus_tag="VF058_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX6207816.1"
                     /translation="MNSLSDESLLAGLAAGDPEGSTAFVRRFQGRVFGLALTIVGDPR
                     VAEDVAQEAFVRAWRHAELYDPARGRVVAWLLRITRNVALDALRVRRATPVDPETLLG
                     MAASEESDPEDRAVRSLELHTLRSDLVALPHEQRRAVLLAAFYGRTAREIGQIEGIPL
                     GTAKTRIRSGMRRLRKALEVRDGRERM"
     gene            10529..11218
                     /locus_tag="VF058_05590"
     CDS             10529..11218
                     /locus_tag="VF058_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207817.1"
                     /translation="MGENGCEMTHGVAAELALSVALGEERDRALRHVETCASCRRDLV
                     ELGELVDELMLLAPEREPPVGFESRVVSTVTEDRRPSPWRRIIASVAAAVIAAAATGT
                     AAYVLTKDERTVAAQFRTALTRAGGQYFGVEFLLEEGGGKAGHVFVYRGDISWAFVVS
                     SGELSGSFRVEMTTRDGRTLEAGAIDPAGEGGGAGLVLPVDLEMIESIRLIPVGGGDA
                     LEAHMPEPPEG"
     gene            11242..11523
                     /locus_tag="VF058_05595"
     CDS             11242..11523
                     /locus_tag="VF058_05595"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX6207818.1"
                     /translation="MAALTPEQVAEGLRGLPGWRHEGGEIFKWFRFGGFPEAIAFLQR
                     IVEPAERMNHHPDVESHYDRVRIGLHTWSEEAVTEKDLRLAREIERLAG"
     gene            11532..12353
                     /locus_tag="VF058_05600"
     CDS             11532..12353
                     /locus_tag="VF058_05600"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016435491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX6207819.1"
                     /translation="MPPEDLTPLVNGWPVEHVAAGITDAESTLGATGDAEWVTRIASV
                     AKLFVGLAALVALEEEAIELDEPAGPEGSTVRHLLAHASGLAFDEHRTIAPPGRRRIY
                     SNTGIEVFADHLAERTGMSFGEYLRLGVLDPLGMSGTELRGSPAHAVWSSVSELLVLG
                     RELLRPMLVAAATLEDATRPHFPELAGVLPGLGRFDPNPWGLTFEIRGDKRPHWTGKR
                     NSPGTFGHFGGAGTFLWVDPAAGLAAVVLTDREFGEWALEAWPAFSDAALNRFAR"
     gene            12417..>12901
                     /locus_tag="VF058_05605"
     CDS             12417..>12901
                     /locus_tag="VF058_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207820.1"
                     /translation="MMRRIVALVCAGVVTLSFGVGPPSGAGEQPRRSWSSFCGSGVSA
                     PCIESATRDGIPVTSMDPIWSVFPESYNEGKSRVVRLHVQKDGSFELGLDALDDVWVI
                     TVRTGSVVPRVVSGYAADATIERFASPNRVTVTASPVRMTDNDECSPIWPWSCPHQAT
                     N"
BASE COUNT         1844 a         4323 c         4754 g         1980 t
ORIGIN      
        1 gaacgacggt tccctggtcg cgaaggcaga gaaggaggtc ggcatggatg tgatgctcgc
       61 cgagacgccc acaccgctcc gcgagggcac ctggagcccg ttgctccgcg ccgccggatg
      121 ggcggccctg gccaccgcgg cgctcatccc gatccagatc gccgtgttcg tcgcgttccc
      181 gtatccggag acgacccagg ggtggttcgg gctgctgcaa ggcaatccgc tcgccgggct
      241 cgtggacctc gacctgctcc tggtggtgga caacgtcctg ctggtggtga tcgccctggc
      301 gctctatgtg gcgctgcgac cggcccggcc gggcgtcatg ctggcggcga ccggtctctg
      361 gctgacggcg ctcgtgctgc tcatcgcctc gaacccggcg atcgagatgc tctcgctgag
      421 cggccgattc gccgaggcga cgacggagac gcagcgagcg gcgacgctgg gagcggggga
      481 ggcggttctg gcgacctggg aggggaccgc gttccaggtt tcttacgtcg tggggcagct
      541 ggccggcatc gccatcggtc tggtcatgct gagggcgggc tcgttcggga gagccatccc
      601 gatcctgatg atcgcgggga acacgatcgg gttcggctac tacctgcccg tggtcgggct
      661 ggcgatctcg gccttctccg gtgtggtgct ctgggcgtgg tatgtgctgc tcggactgag
      721 gctcctccgg atggcgagga gcccaggggg gttcgatgag gtggctgttc gcggtcgtgc
      781 tggtcgggtg agagagggtg aataggatgc gtgacttcgc gcggcgccac gagatcgccg
      841 cctacgtgat gctggcctac gggatctcgt ggatcgtgtg ggcaccggcg gtgctgacgg
      901 gcgcgggggg tatggccctc gtcgctctcg gggcgttcgg tcctccgatc gccgcggggc
      961 tcgtgatcag gtggaccggg ggctcggtcc gcggatggct ccgcccgctc ctgcggtgga
     1021 gggtgcccgg atggtactgg ctgttcgcac tcgggcttcc ggccctgctg ttcgccgtgg
     1081 tgaacctggt gctcaccctg ctgggcgagc cggtcgagct cggccgcctg gcctcggcgc
     1141 tccccgccta catcgcgacg ttcgtggtcg tcgctctggt ggggggaggg caggaggagt
     1201 cggggtggcg aggcttcctc ctggaccgcc tccaggcgcg gttctcgccg ctcgccgcta
     1261 cgctgctgct cggcgtgatc tgggggctgt ggcacctccc gatctacggc atcgggttcg
     1321 tcgggccgat gatgttcgtc ttcttctaca cgtggctgtg gaaccggacc cggagcctcc
     1381 tcctctgcgt cgtgctgcac gggagcttca ccgcggcgct cgacaacctt ttcctggtga
     1441 atgacagcct gaccgtggac ctcaccatcc tggggacgct cgtgggcggc gccgtctcga
     1501 tcgtcctggc gacgcgcggg cggctcggct tcgatcaacc tctcgtggtg gaacggcgcg
     1561 agccggcgcc cgccgaggtg agtggggtgc cggcctaggg ggcggatgat ggatcgactg
     1621 ggcccgcagg acctgatgat ggtctggccc gaggagcttg gctggtcgca ggacatcggg
     1681 gcgatcgcga tcctggacgg ccgcggcttc gcggaggagg acggtgggtt cccgatcgag
     1741 gcgatccggg agcacgtccg ggggaggctg cacctccttc cgcgcttccg ccagcgcctg
     1801 catcggcctc cgctcggcct ggggtggccc ctgtgggtgg acgcgccctc gatcgatctg
     1861 cgaagacacg tccggatgct gccgctggag ggccccgccg acgaggagcg gctgcttgcc
     1921 acctgcgagc ggctgcggcg tcgtcggctg gatcgatcga ggcctctttg ggagctctgg
     1981 ttcctgccgg gcctgacggg cggccgcgtg ggcttctaca tgaggctgca tcacgccgtg
     2041 gccgacggcg tggccggcat cgccgcgctc ggggcgttcg tggaccccgt ccccgaccct
     2101 ccccagacgc cgcaacccac ctggcggccg cgccccacgc ccacggcgat gcagctgctc
     2161 gcggacaacg tgcgatggcg gctggcgacc ctcggccggt tcttcgacaa gctcgcgcac
     2221 ccctcccgga ccctgcgcgc agcccgccgc ggatggccgg ccgtccgcga ggccttcatc
     2281 gaggagcgag ccccccgcac gagcctgaac cgccgggtgg ggtgggatcg tcgactggac
     2341 gtgatccgcg tcgagctgga cgccgtgaag cggatcgcgc acgcgcacgg cgggaaggtg
     2401 aacgacgtgc tcctcgctgc tgtggcgggg ggtgctcgcg cgctgctgct cgggcggggg
     2461 gagcgggtgg agggtctctc gctccgcgcc ttcgtgcccg tgtctctcca cggcgctgag
     2521 acggacgggg cgggaggcaa cctggacggc gccatgatgg cgccgctgcc gatcggggag
     2581 aaggaccccc gtcggacgct tcgaaggatc gctgccgaga ccgccgagcg gaagcggaag
     2641 ccccggcctc cgggcgggac cctcttccgg aacgtgccga tccagcgggc gtggctccgc
     2701 tacgcaccac accagcgggt gatgaacacc tacgtggcca acgtcccggg gcctccggtg
     2761 cctctgtact tcgccggggc gccggtgctc gaggccttcc ccgtcgtgcc gctgatcggc
     2821 aacgtcacga tcggcgtggg ggccctctcc tacgcgggac agttcaacct caccgtcgtc
     2881 gccgaccgcg acgcctgtcc ggacctcgag cggttcgtgg tgggcgcgcg tcgaacgctg
     2941 gacgcgctgg ctcgcgcgac aggcggcgtc gtgggtgacg ggagtgggac cccttccacg
     3001 ggggaaggag ggaagccatg aaggtgctcg tgtcggccgc gtcgaagcac ggctccacgg
     3061 ccgagatcgc ccgcgcggtc gccgatgagc tcgaggggcg tggccaggag gcaacggtga
     3121 tgccgcccga ggaggtctcg tcggtcgagg gctacgacgc gctgatcctg ggtagcgccg
     3181 tctacgccgg ccactggatg aagcccgcgc tggagctcgt cgagcgactc ggggcagact
     3241 tgaagtccct gcccgtgtgg ctcttctcga gcggccccat cggcgatccg cccaagcccg
     3301 aggaagaccc ggtcgacgtg gccgacgtca ccgaggccgc cgacgcccgc ggccaccgcg
     3361 tcttcccggg gaagctcgtc aagaaggacc tcggcttcgg cgagaaggcc atcgtgatgg
     3421 cgctccgagc gccggagggt gacttccgcg actgggacga gatccggggg tgggcgtcgg
     3481 agatcgccga ggcgctggag ggcgggcggt aggcgtcggg cggcggcagg cttcctgccg
     3541 ctcgtcgtcc ccgattaccg accgctcctg gggatcgcct acgcctcgat cgtcctcctc
     3601 ggcgccccct tcgactggcc tccggacgtc gggatcgccg acgtcctgcc ctggccggtg
     3661 gcgaaccagc tcatcctcct ggcgggcggc gtggcatggg cggccgcggc catcggtgcg
     3721 ggaggggatg acccgcccca aggatcgtcg aaagggctcg tggtacggtt ggtcgcctcg
     3781 agggagggag cggaatgcga agggtcggca tcctggtgct cgccgccggc gtcctggtcg
     3841 gcacgacgtc ggcggtcgcc gccgcgaaac ccaagacgcg gctggtcagc aagacgtccc
     3901 agggacaggc cggggacgac acgagcgtgg aggcgcggat ctcgaacgac ggcagatacg
     3961 ttgtcttcca gtcgcaggcg gacaacctgc cgggcccaga cgacgtccag ggcatccacg
     4021 tgcacgatcg caagaccaag aagacgcgcc tcgtgagcag gacgtcgagc gggcagcccg
     4081 ccctcgggca gaaccggaac cccgacatct cggggaacgg gcgcttcgtc gtcttcgagt
     4141 ccgacgcgtc caacctgccg gggaacggct tccaggtgta cgtccgcgac ctgaagacga
     4201 agaagacccg gctcgccagc aggaactcgg cgggccagcc cgccgacgcc tccggtctca
     4261 gcggccggat ctccgactcc gggcggttcg tgaccttctc ctcgatggca gggaacctcc
     4321 ccgcgggcgg aggtcctctg tcgaagctct acatccacga ccggaagaca aggaagaccc
     4381 ggctcctgag caagagctcg aacggcacgc ccgtgagcgc aggcctccac gcgctctccg
     4441 cgagcggacg gttcgcggcg ttcacctcga gcgacccggc gctcccggga ccaagcacat
     4501 tcagggtcta cgttcgcgac cgcaagacga agaagacccg tctcgtgagc aagaggagct
     4561 cgggggtgcc cttcgacgag ttcgcgagct gggtggacat ctccgggagc gggagggtgg
     4621 tcgtcttctc cctcaacata gagttcaacg agatctgcga tagggtcgac atccacgctc
     4681 gcgatcggaa gaccaagaag acgcggctgg tgagcaggac cacggcggga gccgcggccg
     4741 acggctgcaa ccagtggccg gccatctcgg ggaacagcag gtacgtggct ttccactcca
     4801 acgctcccaa ccttcccggg gcatccccgc cgatcgcccg ctcctacgtc cacgatcgga
     4861 agcggcggac gacgagggtg ctgagcaaga ccacggcggg gacggtcgtg accggcgccg
     4921 gtcccgggct ctcctggacg ggggagtggg cgaccttcgt gtccaacgag gcagcgctgc
     4981 ccggcccgaa cacgggcgtc ttccaggtct acgtccgcgg gccgctgcgc tagccgcggg
     5041 cgatccggac gtcttggtcg gcggcgtccc cagccacgaa ggtgaggagg cgctcgccct
     5101 cctcacccac ggcggtggac tcgggcttgc tgagccttcc cagcgggacg atctcgagcg
     5161 tcgcaccctc ccctgatctc gagaccttcc acatgcctcg aacgaacccg tccacgagca
     5221 cgctgcccac gttcccccgc agcatcctct cctgcatccg gtgccgatcc tcgtcctcca
     5281 cgatgcggcg acgatcgtcg tgggacagcg tgacgttgtc gtaggtgggc aggaaccgag
     5341 cggccgcgtc gcggtccgga tcggggagga gcccgtcctc cacgtcgaac agctctcggc
     5401 ctccctcgtt ccggaaggtg cggagccgag gacggaggcg ctcgacgacc tctcgcagac
     5461 cggtcaggcc cgaccacgtc cgcatatcgg cgatcgaggc cggcccgaac gcggcgaggt
     5521 atctgaggat catgcggtcc ggtcgcgcgc tcttccccag gggacgtccc aaccatcgct
     5581 ccgcggtggt caacgtgggg aggccgctcg cgccccacac gcctcggggc ggcacctgca
     5641 ccatcggcag gaggtagcgc gcagcaggga cgagcgaggt ctcgtcgtgg tcgggccagc
     5701 gtttcgcgag cgcctcccgg agctgggcgg cggttctcgg ctgctcctcg acgagcttgc
     5761 gccccgccgc cagcagctcc tcgaggtcga gatccgtgag gttccgtccc cagaggtgtc
     5821 cgtggaagtt gcgctcgagc tggggcttca tgacgggcca catcgcgagg gcgtcccggg
     5881 ccgtgacgag gtggatcgtg ccccgcatca gcgtcaggcg caccgcacgg cgtcggacca
     5941 ggaggtcggc gagatcctcg tgatgaaagc cctccagccg gctccacagc gccacgtagg
     6001 ggtcgggcgg ctcctgggcc tggaggccga cgaggcgctc caccgcccgc ggcacggaca
     6061 gcgcggctcg ctcgagcagg agctgccgtc cgagcagggc tcggttcagt gctcgcctcg
     6121 tcagcacggg catcagcgaa gcatgccggg tcggcgcccc gcgcgcacgc tccgaccctc
     6181 ggttgcggcg ctcgctccgc gggtaactcc ccgggcatgg acgaccgctg gtacaagaac
     6241 gcgatcatct acgagatcga cctccgctcg tacgctgact ccgacggcga cgggatcggc
     6301 gacctccgtg ggctgatcga tcggctcgac tacctcgccg ccctcgggat cacctgcatc
     6361 tggctgctgc cgttctcgcc ctccccctgg agggacggcg gctacgacgt gaccgaccac
     6421 tacggggtcc atcccgagct cgggacgccc ggtgacttcg tggagctcgt gcaccgtgcc
     6481 gcggcccggg gcatccgggt gatcatcgac ctcgtcctga accacacgtc cgatgagcat
     6541 ccgtggttcc aggccgcgcg ggacccggac tcgcccctcc gagaccacta cgtctgggcg
     6601 gacactccgc cggacgacgc cgagcgcggc gtggtgttcc cgggcgtgca ggagtcgacg
     6661 tggaccctcg acgagcgatc gggcaagcac tacttccacc gcttctacga gttccagccg
     6721 gacctcaacg tcgccaaccc cgacgtccgg gccgagttcc ggaggatcct cggctactgg
     6781 atccagcagg gcgtcgcggg gttccggatg gacgcgctcc cgttcctgat cgagccggcc
     6841 ggggtggaac ggccgaagcc ggagccccac ttcgagtacc tgaaggagct ccgggcgttc
     6901 ctccagtggc ggaggggcga cgccgtcctg ctgggggagg cgaacgtcga gcgagaccgg
     6961 gtcgaggact acttcacgac cggcggcatg cacatgatct tcaacttccc gctcaaccag
     7021 ctcgtctggc tggccctggc gaagggggag gctggcccca tcgcccgcgg gctcgagcac
     7081 acggcgggga tcccggccac cgaccagtgg gccaccttcc tccggaacca cgacgaggtc
     7141 gacctggggc ggctctccga aaaggaccga cagatggtct tcgaggcgtt cggccccgag
     7201 ccgtcgatgc agctctacga ccgcggcatc aggcgacggc tggccccgat gctgggaggc
     7261 gaccggcgcc ggatcgagat ggcgatgagc cttctgttca gcctccccgg gaccccggtg
     7321 atcctatacg gcgacgagat cggcatgggc gacctgctct cgctcgacga gcgggaagcg
     7381 gtgcggacgc cgatgcagtg gaacgacgag gagaacaccg ggttctccac cgccgacccg
     7441 gatcgcctgg cccgcccggt gatctccggg ggggactacg ggtacgagcg ggtcaacgtc
     7501 gccgcgcaga tgcgggacgc gggatccctc ctgagctgga tgaagcgcct ggtccacacg
     7561 cggagggagg cgccggaggt gggcctcggc gactgggagc tcctcgagac cgggaatgag
     7621 cacgtcttcg gcctgcgagc ggagtgggag gggcgcgagg tggccgtcta ccacaacctc
     7681 gacggcgagc cgcagaaggc ccggatgggc ctcgagccca cggagctctc gcacccgctg
     7741 gagctcctcg gcgatcgcga ctacgacgag gggtggagcc ccttcgagct ggccgggtac
     7801 ggctaccgct gggcccgggt ccgcggcgtc gcctgacgcc gcgactcctc agcgtacgac
     7861 cacggtcgtc tcctcggcgc tgttgttggc gggatcgggg tcgaagtgga gcggcacggc
     7921 cgttgcccgg ttggccaggg ccgccggctt ccgggccgac acgacgatcc gtaccgtcgc
     7981 cgatccaccg tcggggaccc cgaagatccg gcagacgatc tcgtcgcggg agaccgtgca
     8041 gctcgatcct cccgcgtcca cggcttcgac cctcacaccg gagggcaggg tgtccacgag
     8101 ctcgagcgtc gcccacgtgg gccctcggtt ctcgatcgag atcgtgtacg tcaggggctg
     8161 ccgcacacgc acggggtccg gcgagtcggt caacgagacg gccacgtccg ccgccggatc
     8221 cacatcggtg ttgatgctgg accgattgtc gagggtgttg ggatcgggct cgtttgcctg
     8281 cacggtcacc gtgttcgtca ggtacccgag gtcctgagga acgacgacga ccgaggcgtc
     8341 ggcggtctgg ccgggtgcga gcgagccgag atcgcagcgt acgattcctc ccgactcgga
     8401 gcagccgccg ccctggctcg ccgtggccga atcgaaggtc acgccgtcgg ggagctcatc
     8461 cagcatcgcg acgccggtcg cctccgcggg ccccgcgttc ctgacgcgga cgtcgtaggt
     8521 gatgggctcg ccagcgggca cggggtccgg cgaggcggcc tgggagattg agaggtcccc
     8581 cgtggcctcc gagggaggct ccggcccacc cagctccttc acgtacacgt cgggaccgga
     8641 ttctccatcc gcgggatcga ggttggtcgc gtcggaccgg aacgccaccg cagccccccg
     8701 atctccgagc gacacctggt agctcgtgcc gttccctttg atcccggacg aggacgtcga
     8761 cgccagctcg acgccccccg tcgacaggtc cttcacgaag atgtcggaca cgacgtccgc
     8821 gtcggcaggg tgcaggttgg tcgcgccgga gctgaaggcc acgcgggtgc cgtcggcgga
     8881 gagcgaggga tccgccgcgc cacggtccgc acgctcgccc gaggccgata gcgaggctgg
     8941 ccgaacgtcc ccggtcgcga ggtccttgac gaagatctgt ccgaagcccc cggagaaacc
     9001 cggcacgagg ttcgaggagt acgagcggaa ggccgcgatg ctgccgtcgg ccgagagcga
     9061 ggggctgaac gagttgccgt cgcccttgac cccatcgggg gcggtggaga tcagccggag
     9121 ctcaccggtc tccagattct tcacgtaggc gtcgaagatg tcgtcgccgt cgccgggatg
     9181 gaggttcgtg gcgccggagg cgaaggcgac catgataccg tccccggaca gcgccgcgcc
     9241 caccgacccg tggaagaagt cgccgttcgc cttgatgccc tccgaggagg tcgaggcgag
     9301 gaagagttcg cccgacacca gatctcggac gtagatgtcg gctgtctcgt cgtcgtcgcg
     9361 gggatcaagg cccgtggaga tcgtcgagaa ggcaacgcgg gtcccatcaa tcgacagggc
     9421 tggatactcg ccgcccggcg cgacgaggaa gacctctccg gtggtcaggt ccttgaccat
     9481 cagcccggtc gctccggatg gatgcaggtt cgtggcgccg gagaggaaag ccacccggtt
     9541 gccgtccgcc gagatcgccg cgtgggagct ccgcgcgttt cccttctccc cgctcgccga
     9601 ggtcgaggcg agccagatct cgctggtggt caggtccttg acgtaggcat cggtctccgc
     9661 gtcgtcgtcg tcaggatgaa gctgcgagcc ggatgagaac gcgacccggg tcccgtcgcc
     9721 cgacagcgag ggctgggtgc tccattctcc cttcacgccg tccgcagtga tcgaggcgag
     9781 gtgaaggtcc cctggagcac ccgccgcccg gtaggggacg aggaccaggg agaggacggt
     9841 gagggcgagg agcgcgcggc gcatggggcc tccttccgtc ggtgacgacg tcgcaggtac
     9901 tactgaggga ggggtcagaa gggattgcac gacgaggcgc ctgctgagca atccattggg
     9961 gggcccgggc ccgtatatcc tgcggtgaac tcgctctccg acgagtcgct cctggcgggt
    10021 ctcgcggcag gcgatccgga ggggtcgacg gcgttcgtgc ggcggttcca gggtcgcgtc
    10081 ttcgggctcg ccctgacgat cgtgggcgac ccgagggtag cggaggacgt cgcccaggag
    10141 gcgttcgtgc gtgcgtggag gcacgctgag ctctacgatc cggctcgggg ccgggtggtg
    10201 gcgtggctgc tgcggatcac gcggaacgtc gcgctcgatg cgctccgcgt gcgccgggcc
    10261 acgccggtgg atcccgagac gctgctgggg atggcggctt cggaagagag cgatccagag
    10321 gaccgtgcgg tcaggagcct cgagctccac accttgcggt cggatctggt cgcgctaccg
    10381 cacgagcagc gccgggcggt gctcctggcc gccttctacg ggcgcacggc gcgagagatc
    10441 gggcagatcg aggggatccc gctgggcacc gcgaagacgc ggatccggtc ggggatgagg
    10501 aggctgagga aagcgctcga ggtgcgggat gggcgagaac ggatgtgaaa tgacgcacgg
    10561 ggtcgcggcg gagctggcgc tcagcgtggc cctcggcgaa gagcgggacc gggcgctccg
    10621 acacgtggag acctgcgcct cgtgccgtcg agacctcgta gagctgggcg agctcgtgga
    10681 cgagctgatg ctgctcgctc cggagcgaga gcccccagtg gggttcgagt cccgggtggt
    10741 gtcgacggtg acggaggatc gtcggccttc cccctggcgg cggatcatcg cgtccgtcgc
    10801 ggcggccgtg atcgcggccg ccgcgacggg gacggcggcc tatgtcctga ccaaggacga
    10861 gcggaccgtc gccgcgcagt tccggaccgc gctgacccgg gccggcggcc agtacttcgg
    10921 cgtcgagttc ctcctcgagg aggggggagg gaaggcgggc cacgtcttcg tgtaccgagg
    10981 ggacatctct tgggcgttcg tcgtctcgtc gggggagctc tccggcagct tccgggtcga
    11041 gatgaccacc cgcgacggac gcacgctcga ggcgggagcc atcgatcccg cgggcgaggg
    11101 cgggggggcc ggcctcgtcc tgccggtcga cctggagatg atcgagtcga tccgtctcat
    11161 cccggttgga gggggggacg ccctcgaggc gcacatgccg gaaccccccg agggctaatc
    11221 cgagcgggta cgatccggcg catggccgcg ctgacccccg agcaggtcgc cgaaggcctc
    11281 cggggtctcc cggggtggcg gcacgagggc ggggagatct tcaagtggtt ccgcttcggc
    11341 ggcttcccgg aggcgatcgc cttccttcag cggatcgtgg agcccgccga gcggatgaac
    11401 caccatccgg acgtcgagag ccactacgac cgcgtgcgga tcgggctgca cacgtggagc
    11461 gaggaggccg tcaccgagaa ggacctcagg ctggcccgtg agatcgagcg gttggccggg
    11521 taggctcgag gatgcccccc gaagacctca cccccctcgt gaacggctgg cccgtggagc
    11581 acgtggcggc ggggatcacg gacgcggagt cgacgctcgg cgcgaccggc gacgccgagt
    11641 gggtgacgcg gatcgcatcg gtggcgaagc tgttcgtggg gctggccgcc ctcgtcgccc
    11701 tggaggagga ggcgatcgag ctggacgagc ccgcgggccc cgaggggtcc acggtgcggc
    11761 acctcctggc ccacgcctcg gggctggcct tcgacgagca ccggacgatc gcgccgcctg
    11821 gacgccggcg gatctactcg aacacgggga tcgaggtgtt cgccgatcac ctcgcggagc
    11881 ggacggggat gtcgttcggg gagtacctgc gcctcggcgt gctggatccc ctcggcatgt
    11941 ccggcacgga gctcaggggc tccccggctc acgcggtgtg gagctccgtc tcggagctgc
    12001 tggtgctggg gagggagctg ctccggccca tgctcgtggc ggcggccacg ctggaggacg
    12061 ccactcggcc ccacttcccg gagctggcgg gggtccttcc cggcctgggc cgcttcgacc
    12121 ccaacccctg gggcctgacc ttcgagatcc ggggggacaa gcggccccac tggacgggga
    12181 agcggaactc tccggggacg ttcgggcact tcggaggggc ggggaccttc ctctgggtgg
    12241 accccgccgc gggtctggcc gccgtcgtgc tcacggatcg ggagttcggc gaatgggccc
    12301 tcgaggcgtg gccggcgttc tcggacgccg cgctcaaccg attcgcccgg tgacccgaca
    12361 catacctgag ggtcggcccc tggtagcatc cccgggcact ttgcgaggag ggagcggtga
    12421 tgaggcggat cgtggcgttg gtgtgcgcgg gtgtggtgac gctctccttc ggggtcggcc
    12481 caccgtcggg tgccggggag cagccccgca gatcgtggtc gtccttctgc ggatcggggg
    12541 tttccgcccc ctgcatcgag tccgctaccc gtgatggcat cccggttacc tcgatggacc
    12601 ccatctggtc cgtcttcccc gagtcgtaca acgaagggaa gtcacgggtc gtccgcctgc
    12661 acgtccagaa ggacggcagc ttcgagctgg gactggacgc gctggacgac gtctgggtga
    12721 tcacggtgcg gacggggtcg gtcgttcccc gcgtggtgtc cggctacgct gccgacgcca
    12781 cgatcgagcg cttcgcgtcc ccgaatcgtg tgacggtgac cgcctctccc gtgcggatga
    12841 ccgacaacga cgagtgcagc ccgatctggc cgtggtcctg cccgcaccag gcgacgaacg
    12901 a
//
LOCUS       DASUTW010000163         3335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_568918, whole genome shotgun sequence.
ACCESSION   DASUTW010000163 DASUTW010000000
VERSION     DASUTW010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568918"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1228)
                     /locus_tag="VF058_05610"
     CDS             complement(131..1228)
                     /locus_tag="VF058_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HEX6207821.1"
                     /translation="MIRLRHGVVRSVEAPRPGLLELGVEVEGRSELAIAYPELTGPVR
                     EGDRVLLNTTAVAEGLGSGGYHFVLAVEGAPDADPPGEGHVMKLRYTPLQAKVLAVEE
                     QDSPDREAVVDADSLDGLPVAWIPLHSMLGAACAGARAAGARRIAHVMTDGAALPIWL
                     SRQVHELREAGLLDATITAGQALGGDREAVNVFSALLAAREIEEADVVIVGDGPGKVG
                     TATRWGATEVSGGLALQAAAILGARAIAAVRINFADPSYRHHGLSPHSLTVLTRVASP
                     GAHVAVPLLEGRRRDAVWEAIREAKLEEQHQVVEVTGQPALDLLAERGVAAETMGRKL
                     EDEPEFFLAAGAAGVLAGRMAAGTERWKAGT"
     gene            1299..2249
                     /locus_tag="VF058_05615"
     CDS             1299..2249
                     /locus_tag="VF058_05615"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX6207822.1"
                     /translation="MEPLERLLKLVALLLNAPRPLTFDEIRDEMGAYTQDDRDVAKRQ
                     FERDKDDLRRIGIPIETGGTDVYEVEVGYHIPKERYYLPEISFTPEEIAALFIAAHAP
                     GPYEEAAQAFGKLARDADTAVISGLVDRRVPGLDDSSVNVMDAADAVARRRLVRFAYR
                     PTGGEVSDREVDAWGLVCSRGAWYLVGRDRSAEDVRSFRLSRIVEGLEDAGDAEAPPE
                     RFDARDRLRSGPWGVGEPQTTATVAFSPKVAWWARDQAPGSTVVETRADGWTVLEVPA
                     STDESFLGWVRAFGPDAELLAPEDLRRWLAGSLEEIRASL"
     gene            2236..3174
                     /locus_tag="VF058_05620"
     CDS             2236..3174
                     /locus_tag="VF058_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX6207823.1"
                     /translation="MPPSKVARRIERLLAIVPYVIRHPGTTLEELGRLFDVDPRSLTK
                     DLNLLFVTGLPPYDPGNLIEVDIEDGRVWIGMADYFQRPVRLTPAEALALYLKGTVFL
                     GAQGLSEADALRSALDKLKEHLGETLGGLQIEAGEGKPSGPLDAVRGAVERRETLEID
                     YYSASRDEVTTRRIDPEHVFSALGNWYAVAWDHLRDGERLFRIDRIREARRTGDPFEP
                     RGLAGAGRPLYTPTGRDVAVRLRLGPRARWVAEYYEVDDEQEVDGGLEVTLPTPDLAW
                     VAKLVLRVGTDVEVLDPPELRETARAMAEEALARYA"
BASE COUNT          444 a         1242 c         1209 g          440 t
ORIGIN      
        1 gcgccggccg acagcgccgc ctcggcgctg gtcgacgagt ccacgacgtc caccacgcac
       61 acgacccggc cgcgggcccc ggcctcggcc gccccgctcg cgttgaaggt cacctcgagc
      121 gtcgggacca tcaggtcccc gccttccagc gctccgtccc ggccgccatc cggcccgcca
      181 gcacgccggc cgcgccggcc gccaggaaga actccggctc gtcctcgagc ttccggccca
      241 tcgtctccgc cgccacaccg cgctcagcca ggaggtcgag cgcgggctgc ccggtcacct
      301 cgacgacctg gtgctgctcc tcgagcttcg cctcccggat cgcctcccag accgcgtcgc
      361 gccgccggcc ctcgaggagc gggacggcca cgtgcgcccc ggggctcgcc acgcgtgtca
      421 ggaccgtgag cgagtggggg gagagcccgt ggtggcggta gctgggatcc gcgaagttga
      481 tccggacggc ggcgatggcc cgtgcgccca ggatggccgc ggcctggagg gccagcccgc
      541 cggacacctc ggtcgctccc cagcgggtcg cggtgcccac ctttcccggg ccgtcgccca
      601 cgatgaccac gtccgcctcc tcgatctcgc gggccgcaag gagggcggag aagacgttca
      661 cggcctcccg gtcgcccccc agggcctgcc ccgccgtgat cgtggcgtcg aggaggcccg
      721 cctcccgcag ctcgtggacc tggcgcgaga gccagatggg gagagcggcg ccgtcggtca
      781 tcacgtgcgc gatccgccgg gccccggcag ccctggcccc ggcgcaggcc gcgccgagca
      841 tcgagtggag ggggatccag gccacgggga ggccgtccag cgagtcggca tccaccaccg
      901 cctcgcgatc cgggctgtcc tgctcctcga cggcgagcac cttcgcctgg agtggcgtgt
      961 agcggagctt catgacgtgc ccctcgccgg ggggatcggc atcgggggcc ccctcgacgg
     1021 ccagcacgaa atggtagccg ccgctgccca gcccctcggc gacggccgtc gtgttcagga
     1081 gcacgcggtc gccctcccgc acggggccgg tcagctcggg gtaggcgatc gcgagctcgc
     1141 tcctcccctc cacctcgacg ccgagctcga ggaggccggg gcgaggggcc tccaccgagc
     1201 gcacgacgcc gtgcctgagt cggatcaccc gcgtatcgta gacggcccga gccggcgccc
     1261 gtcgcccccg cccccggact gggtactatc gggccgcgat ggagcccctg gagcgcctcc
     1321 tcaagctggt cgccctcctc ctcaacgccc cccgtcccct cacgttcgac gagatccgcg
     1381 acgagatggg ggcctacacg caggatgacc gcgacgtcgc caagcgccag ttcgagcggg
     1441 acaaggacga cctccgccgg atcgggatcc cgatcgagac cggcggcacc gacgtctacg
     1501 aggtcgaggt gggctaccac atcccgaagg agcggtacta cctgcccgag atctcgttca
     1561 cgcccgagga gatcgcggcc ctcttcatcg ccgcgcacgc tcccggcccc tacgaggagg
     1621 cggcccaggc cttcgggaag ctggcccggg acgcggacac ggcggtgatc tcggggctcg
     1681 tcgatcgccg ggtgccgggg ctcgacgact cgagcgtgaa cgtgatggat gccgcggacg
     1741 cggtggcccg ccggcgcctc gtccgcttcg cctaccggcc gaccggcggc gaggtatccg
     1801 atcgggaggt cgacgcctgg ggcctcgtct gctcccgggg agcctggtac ctggtgggac
     1861 gtgaccgctc ggccgaggat gtgcggagct tccggctctc gcggatcgtg gagggcctcg
     1921 aggacgccgg cgacgcggag gcgcctcccg agaggttcga cgcccgggac cggctccgga
     1981 gcgggccctg gggagtcggg gagccccaga ccaccgccac cgtggccttc tcgccgaagg
     2041 tggcatggtg ggcccgggac caggctcccg gctcgacggt ggtcgagacg cgcgccgacg
     2101 ggtggacggt gctcgaggtc ccagcctcga ccgacgagtc gttcctgggc tgggtccggg
     2161 cgttcgggcc ggacgccgag ctcctggcgc cggaggatct ccgccggtgg ctggccggat
     2221 ccctggagga gatccgtgcc tccctctaag gtggcccgcc gcatcgagcg cctcctggcg
     2281 atcgtgccct acgtgatccg ccaccccggc acgaccctcg aggagctcgg ccggctcttc
     2341 gacgtggacc cgcgctcgct caccaaggac ctcaacctgc tgttcgtgac ggggctcccc
     2401 ccgtacgacc ccggcaacct gatcgaggtg gacatcgagg acggccgggt gtggatcggg
     2461 atggccgact acttccagcg gccggtccgc ctgacaccgg ccgaggccct cgccctgtac
     2521 ctgaagggga cggtcttcct cggcgcgcag ggtctgagcg aggccgacgc cctccggtcg
     2581 gccctggaca agctcaagga gcacctgggc gagacgctgg gcggcctcca gatcgaggcg
     2641 ggggagggga agccctccgg tccgctggat gccgtgcgag gggccgtgga gcggcgggag
     2701 accctggaga tcgactacta ctcggccagc cgcgacgagg tgacgacgcg caggatcgat
     2761 cccgagcacg tcttcagcgc gctcggcaac tggtacgcgg tcgcctggga ccatctccgc
     2821 gacggggagc ggctgttccg gatcgaccgg atccgcgagg cgcggcgcac cggggacccg
     2881 ttcgagccca gggggctggc cggcgccggg cggcccctct acacgcccac cggtcgcgac
     2941 gtagccgtcc gcctccgcct cgggcctcgg gcccggtggg tggcggagta ctacgaggtg
     3001 gacgacgagc aggaggtcga cggcgggctc gaggtcactc tccccacccc cgacctggcc
     3061 tgggtggcga agctcgtgct gcgcgtgggg accgacgtcg aggtgctcga cccccccgag
     3121 ctgcgggaga cggcgcgggc catggctgag gaagcgctcg ccagatacgc ctgagcccgg
     3181 cccgtctaaa gtcgcctccc cgcttgccga tacggatggt gagatgacca cgatccgcgc
     3241 gacgtgtccg cgatgcggcg aggtggagat gggcgccgcc tccgtcgtcc tcgtcgtcga
     3301 ggccgggacc accgagggaa cctaccggtt cgtct
//
LOCUS       DASUTW010000164        10220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_439038, whole genome shotgun sequence.
ACCESSION   DASUTW010000164 DASUTW010000000
VERSION     DASUTW010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10220
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439038"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /locus_tag="VF058_05625"
     CDS             complement(<1..1311)
                     /locus_tag="VF058_05625"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX6207824.1"
                     /translation="MTARRRITTYVGLVCIAGLPLFAWLMATNGLSAARIAFWTLWPF
                     TAFVVVGQFLPVRIPREGEEEEEITTSSTFSFAILLATGPAPAALALAVGSFASDLMR
                     RKPPWKAGFNVAQYTLSIMAAHLVLETIGFTYLFQGDSAKVEMHTLNLLAVLLAAVAY
                     FVVNNTVTGIAPALAQGLPVGPYLRRDFGVQASIAAVLLALSPVVVVAAQHSLLMVPL
                     LGLPMAAVYKGARVWLEKESQEHQAKHDALTGLPNRALFYQRVRREIHRRAKKPGSFG
                     VMIIDLDRFKEINDTLGHYAGDQLLRQIGPRMRDVLRDEDVIARLGGDEFAVLMPDLP
                     DEMAALQVAERLQEALETPFTVEGLLLDEDASIGIAIYPGHGVSLDDLVRRADVAMYL
                     AKETHSGFELYAPERDQHSLGRLTLLGELRRAIERDQLVLFFQPK"
     gene            complement(1629..1937)
                     /locus_tag="VF058_05630"
     CDS             complement(1629..1937)
                     /locus_tag="VF058_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX6207825.1"
                     /translation="MQLTPTAAEKVKELLAQEGHPEAALRVAAQPGGCCGYRYSMALT
                     DQIDEADQTVEHHGVRVVIDSESVPLLDEATIDFVDSLERSGFAIENPNAGGGCTCGH
                     "
     gene            complement(2317..3969)
                     /locus_tag="VF058_05635"
     CDS             complement(2317..3969)
                     /locus_tag="VF058_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004985424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6207826.1"
                     /translation="MTTTVERPAVRAGTSAFTGTGLLARAIVRRDRVRLGVWVGAIAL
                     SVVAFVASLPQYYTDAAQRQARAELMANPGLRAIAGPGYGLDDYTFGAMVAQEFLSWT
                     AVFVALMSFLLVVRHTRAEEETGRAELVRSAALGRHAHTAAPLVVLVGANVLLGVLLA
                     VGLGSLGMEGVGWGSSWLFGAALASVGVVFAAVAAVTSQVSSHARGAAGLAGFGFAFA
                     YLIRAAGDMSEVGGGFLSWLSPIGWAQQTRVYVDDRWWPLVPSVALAAVLVLVAFRLS
                     TRRDFGAGLVQPRPGPASGSRLLSSPLGLAWRLQRTSVMWWSAAMFAFGLGYGALAGE
                     VERYVGELSEVVQDWLEDIGGDAVIDSWLAVLALLVGVIVAVFAVLSVLRPRSEETAG
                     RAEPVLAASVSRVGWVASHLAVSLAGSVVLLALAGLGLGISASAALGDASVMPRLLGA
                     TLVYIPAVWVTVGIGVALFGLVPRATRLVWIVIAYAGLIGMFAELLGFPEWTVDVSPF
                     GHVPLVPAVEVAWTPLLVLTAIAAVLTALGLYGFRRRDLAAT"
     gene            complement(3966..4880)
                     /locus_tag="VF058_05640"
     CDS             complement(3966..4880)
                     /locus_tag="VF058_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018839933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6207827.1"
                     /translation="MAIAISITDLVKTYGSTVALDGLDLEVPAGEVHGFLGPNGAGKT
                     TTIRILLGLLRADRGTVRVLDGDPWEDAVDLHRRIAYVPGDVELWPNLTGGETIDLLG
                     RLRGGLNPTRRDEMIERFDLDPTKKGRTYSKGNRQKVAIVAALASDAELLLLDEPTAG
                     LDPLMEVVFQDVIRRLKAEGRTVFLSSHILAQVEKLADRISIIRLGRIVQSGTLAEMR
                     HLTRTTIEAHTATPVTGLDALPGVYHVEATDARVRFAVDGEHLDAVVKHLSGFGIRAL
                     TSHPPTLEELMLRHYDDELEGAGAGGSE"
     gene            complement(4948..5781)
                     /locus_tag="VF058_05645"
     CDS             complement(4948..5781)
                     /locus_tag="VF058_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX6207828.1"
                     /translation="MPEYPEVQALADRLDDVFRGAELEGIDAIQFSALKTVEPSPESL
                     IGRPLESVGRRGKWLLFDLGGPRIALHLSQGGRVTLEDPPKRSRPRGGVVRLRFARRP
                     GLMVREFGTERRAAWHVLAPGDEGPLAVLGPEATSEAFVSWLREAEDTRRVHTILRDQ
                     RTVAGIGRGYSDDILHRAKLSPFASLAKLDAEERARLSEAVREVLAEGLEKERKRVGG
                     LPPKLGEHFAVHGRWGRPCPRCGDDLRRISYESHEVTYCPRCQTGGKVLADRRFSRLV
                     R"
     gene            complement(5808..6113)
                     /locus_tag="VF058_05650"
     CDS             complement(5808..6113)
                     /locus_tag="VF058_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEX6207829.1"
                     /translation="MLEVTEAAVGVIRQIRMDTPGVSDEAKARIQLVASGEEEQPGLG
                     FGFSDEAIEGDHKVAEKEDIQVFLAPELVEPLSAAVVDAVPAAEGQQLVLREQDVEG"
     gene            6210..6815
                     /locus_tag="VF058_05655"
     CDS             6210..6815
                     /locus_tag="VF058_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX6207830.1"
                     /translation="MVRTDRGEETRRRILTEAAAAFAERGYAGTSMNELVRAPGLTKG
                     AFYFHFDSQEDLALEVFRWKHEEWQAMVAEAIEEGARAVDQLVAVMGAVTDCVDADPS
                     ARSLGRLAGELSGDPRLASVLDVQFKSWVEFSTELLRRGQEEGDVRHDLDPREVSEVV
                     VAGFIGLEHVSGTRGAGDLRRWSEALTGLVLDAIRAEEQGT"
     gene            6812..9112
                     /locus_tag="VF058_05660"
     CDS             6812..9112
                     /locus_tag="VF058_05660"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX6207831.1"
                     /translation="MKGIGRSVFHRLGGFAVRRRWWVVAVWVAALVAAFVPLRELGGR
                     LSQGGFEVPGSESERVQLAVQSEFTGQFEFSDQLVMRSESVPVDDPRFREAFEDVRAA
                     LVEAPGVGDVTDPYATPERSIGPDGRTLVSTVGLTDDQDQALEHADELHDAVHGAVPH
                     GGEIEVYLTGAAPFYSEFSEVTQHDLERAEAIALPISLLILIAAFGSLVAAGMPLALA
                     LFGLAISFGIISLVAATTTVSIFTQNVASMIGIGVGIDYSLFVVSRYRQELGKGHDVP
                     EAVARAMATSGKAVMVSALTVVIALSGTLLVDIAAFRSMGLGAMIAVAVAGLAALTIL
                     PALLGFVGRKINSLRLGRRRLKDEERSGGLWHRWAMLVMRRPWFALIGSLALLALLAL
                     PARNLRLGTSGPDIFPPDSEPRVAAEAVAGAFGEGQAAPMQILIEHPEGVLESGFEDV
                     AEVAGALQSDPGIVQVDSIATLAPVASVEEARAVAASPEAAPVVQTMVGNEGKATLLR
                     VVTADDPGSDEVRELVRRLREELPALLPAGAEVAVGGDPGLTVDLNDEIEGSIIPVVA
                     MVLTLSFFLLLLFFRSILLPLKAILMNLASVLATYGIVVFIFQEGHFEDLLGFSATGH
                     IESFVPLFLFTILFGLSMDYEVFLLARVREEYLRTGDNTEAVGWGLEHTARIITSAAA
                     IMVTVFGAFAFASLVPIKSMGFGLAAAVFLDATLVRVVLVPATMRLMGDWNWWLPGWL
                     DRILPNVSLEGAEPEPAGPAEAPATA"
     gene            9226..10089
                     /locus_tag="VF058_05665"
     CDS             9226..10089
                     /locus_tag="VF058_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HEX6207832.1"
                     /translation="MTDPDRLSISEVSRFVDVPIPTIRSWERRYGVPAPARTGGGHRR
                     YTREEVDLIRAMRDEIVRGASARDAARRIRLAPAPAVEPTPHLETLLDRVLDLDDAGI
                     RWILDQAADGLGIERAVVEVALPALREVGDRWAGGRCDVAQEHLATNEVRTWIARLGR
                     TAPPPFRPAPILLATAPDETHATGLEAFALLLARRGWPTVVLGARTPVDSLLRTVSKV
                     GPAAVVLSSQWTRARRSALEALEAVAATSVPVFYAGAAFAVSSKREGVPGVYLGDDLL
                     GSLDELERTVA"
BASE COUNT         1446 a         3647 c         3696 g         1431 t
ORIGIN      
        1 cttgggctgg aagaagagga ccagctgatc ccgctcgatc gcccgccgca gctcgcccag
       61 cagcgtcagc cgccccaggc tgtgctggtc gcgctccggg gcgtagagct cgaagccgga
      121 atgggtctcc ttggccaggt acatcgccac gtcggctcgc cgcacgaggt cgtcgaggct
      181 caccccgtgc cccgggtaga tcgcgatccc gatgctggcg tcctcgtcga ggagcagccc
      241 ctcgaccgtg aacggcgtct ccagggcctc ctggagccgc tcggccacct ggagggcggc
      301 catctcgtcg ggcagatcgg gcatcagcac ggcgaactcg tcgccgccca ggcgcgcgat
      361 cacgtcctcg tccctgagga cgtctcgcat ccggggcccg atctgccgca ggagctgatc
      421 gcccgcgtag tgcccgagcg tgtcgttgat ctccttgaag cggtcgagat cgatgatcat
      481 cacgccgaag gagcccggct tcttggcccg ccggtggatc tcgcgccgca cccgctggta
      541 gaagagcgcg cgattgggga gcccggtgag agcgtcgtgc ttcgcctggt gctcctgaga
      601 ctccttctcc agccacacgc gcgccccctt gtagacggcg gccatcggga ggccgagcag
      661 gggcaccatc aggaggctgt gctgggcggc caccaccacg acgggggaga gggcgagcag
      721 cacggcggcg atgctcgcct ggaccccgaa gtcccgacgg aggtacggcc ccacgggcag
      781 gccctgggcc agggccgggg cgatcccggt cacggtgttg ttgaccacga agtaggccac
      841 cgcggcgagc agcacggcaa ggaggttgag cgtgtgcatc tcgaccttgg ccgagtcgcc
      901 ctggaagagg tacgtgaagc cgatcgtctc gaggacgaga tgggcggcca tgatcgagag
      961 cgtgtactgg gcgacgttga accccgcctt ccacgggggc ttccggcgca tcaggtcgga
     1021 ggcgaaggat cccacggcca gggcgagcgc ggcgggggcc ggtccggtcg ccaggaggat
     1081 ggcgaacgag aaggtcgagg aggtggtgat ctcctcctcc tcttcgccct ctcgggggat
     1141 cctgacgggg aggaactgac ccaccaccac gaaagcggtg aacggccaga gggtccagaa
     1201 ggcgatccga gcggcgctca ggccgttcgt ggccatcagc caggcgaaga gggggaggcc
     1261 cgcgatgcac accaggccca cgtaggtggt gatgcggcgt cgcgcggtca cggacgcccc
     1321 ccttcgcgga acgtgcggac gccccgggga ggcggagccg gggcgtccga cggctaccac
     1381 ttgcgctccg cgctccagag ggtcacgagc accgtcagga caccgagcgc gaacgtcacc
     1441 cggagcaacc gggtcgacat gcccacctcc tctctcagcc aaccggacgt ccgaaggcgt
     1501 gctggtccta tcggtcgtcc gggcgacctc cttgagaatc tcgccctatg ggcatgcggt
     1561 cggagccgta gggagaacca ccgatggggg cgaggcgagc cgaagggggc gggcgggctg
     1621 cggcggcctc agtggccgca ggtgcagccg cccccggcgt tggggttctc gatggcgaag
     1681 ccgctccgct ccagcgagtc cacgaagtcg atcgtggcct cgtcgaggag cgggacgctc
     1741 tcggagtcga tcaccaccct gacgccgtgg tgctcgacgg tctggtccgc ctcgtcgatc
     1801 tgatcggtga gtgccatcga gtagcggtag ccgcagcagc cgcccggctg ggcggccact
     1861 cgcagcgcgg cctccgggtg cccctcctgg gccaggagct ccttcacctt ctcggcggcg
     1921 gtcggcgtca gctgcatgcc atctcctcgg atcggcgtcg ggtctgatga tagcggagtc
     1981 gccgcggatg ccgcgggagg gaacgggtgg ggccgggatc gacttcgtcg atcccggccc
     2041 caccttcgct gctccaccgc gagcgcttcc gccttccggc ttcgcccggc ccgaccacgc
     2101 cggggcccgc gatgagggtg atctggttgg attccgccgg ggctctcgtc ccggcgcacg
     2161 ggcggggagg tgacccctac ccgcggaggg acggcctcag cagccagtca cctcgggggt
     2221 cgtcgaacgt ctatgcacga tcggaccacc tcctttctcc cgtgccaccc atcttgcgat
     2281 ggacccggcg cgttggcaag gaggccggag gagtcgtcag gttgcagcga ggtcccgccg
     2341 ccggaacccg tagagcccca gggcggtcag caccgccgcg atcgcggtga ggaccagcag
     2401 gggcgtccac gccacctcca ccgcggggac gagcgggacg tgcccgaacg gcgagacgtc
     2461 gacggtccac tcggggaacc ccagcagctc ggcgaacatg ccgatcaggc cggcgtaggc
     2521 gatcacgatc cagaccagcc gcgtggcccg cggcacgagg ccgaacaggg cgacgccgat
     2581 ccccaccgtc acccagaccg cggggatgta cacgagggtg gccccgagca gcctcggcat
     2641 cacggaggcg tcccccagcg cggccgatgc gctgatcccc agacccaggc cggccagggc
     2701 caggagcacg acgctgccgg cgagcgagac ggcgaggtgg ctcgccaccc atccgacccg
     2761 ggacaccgag gcggcgagca cgggctcggc ccgcccggcc gtctcctcgc tccgcggcct
     2821 cagcacggac agcacggcga acaccgcgac gatgacgccg acgagcaagg ccaggaccgc
     2881 cagccacgag tcgatgacgg cgtcgccgcc gatgtcctcc agccagtcct gcaccacctc
     2941 ggagagctcc ccgacgtacc gctcgacctc gccggcgagc gccccgtacc ccagcccgaa
     3001 ggcgaacatc gccgcgctcc accacatgac gctcgtcctc tgcaggcgcc aggccagccc
     3061 gagcgggctg gagagcaggc gggatccgct cgccgggccg gggcgcggct ggaccagacc
     3121 cgccccgaag tcccgccgcg tcgagaggcg gaacgccacg agcacgagca cggccgcgag
     3181 cgccaccgat gggacgagcg gccaccagcg atcgtccacg tagaccctcg tctgctgggc
     3241 ccagccgatc ggggagagcc acgagaggaa gccgcccccg acctcgctca tgtcgcctgc
     3301 ggcgcggatg aggtaggcga aggcgaaccc gaagccggcc aggccggcgg cgccccgcgc
     3361 gtgcgaactc acctgcgacg tcaccgcggc gaccgcggcg aacaccacgc cgaccgaggc
     3421 gagcgcggcg ccgaagagcc acgagctacc ccaccccacc ccctccatcc ccagcgatcc
     3481 gagcccgacg gcgagcagaa cgccgagcag aacgttcgcg ccgacgagga cgaccagcgg
     3541 agccgctgtg tgcgcatggc gcccgagggc cgcggagcgg accagctcgg cccgcccggt
     3601 ctcctcctcg gcgcgggtgt gccgcaccac gaggaggaag ctcatcagcg ccacgaacac
     3661 ggcggtccag ctcaggaact cctgcgcgac catcgccccg aacgtgtagt cgtcgagccc
     3721 gtacccgggc ccggcgatgg cccggagacc ggggttcgcc atcagctcgg cccgggcctg
     3781 tcgctgcgcc gcgtcggtgt agtactgggg gaggctcgcc acgaacgcca ccaccgagag
     3841 ggcgatggct cccacccaca cgccgagccg gacccgatcc ctccggacga tcgccctcgc
     3901 cagcagcccc gttccggtga aggccgaggt tccggcgcgg accgcgggac gttcgaccgt
     3961 cgtcgtcact cgctccctcc ggcgcccgct ccctccagct cgtcgtcgta gtgccggagc
     4021 atcagctcct ccagggtggg cgggtggctg gtcagggcgc ggatcccgaa cccggagagg
     4081 tgcttcacca cggcgtccag gtgctccccg tccaccgcga agcggacccg cgcgtccgtg
     4141 gcctcgacgt ggtacacgcc cgggagcgcg tcgagcccgg tgaccggcgt cgccgtgtgg
     4201 gcctcgatcg tggtccgggt gaggtggcgc atctcggcga gcgtccccga ctggacgatc
     4261 cgccccagcc ggatgatgct gatccggtcg gccagcttct ccacctgggc caggatgtgg
     4321 ctcgagagga acacggtccg cccctcggcc ttcaagcgcc ggatcacgtc ctggaagacg
     4381 acctccatca gcgggtcgag accggccgtg ggctcgtcga gcaggagcag ctccgcgtcg
     4441 gaggcgagcg ccgcgacgat ggccaccttc tgccggttcc ccttcgagta ggtccggccc
     4501 ttcttcgtgg ggtcgaggtc gaaccgctcg atcatctcgt cgcggcgggt ggggttcagg
     4561 ccgccccgca gccgtcccag gaggtcgatc gtctccccgc ccgtgaggtt gggccagagc
     4621 tccacgtcgc cggggacgta ggcgatccgc cgatgcaggt ccacggcgtc ctcccaggga
     4681 tcgccgtcca gcaccctgac cgtcccccgg tccgcgcgga gcagcccgag gaggatgcgg
     4741 atcgtcgtgg tcttccccgc gccgttcggc cccaggaagc cgtggacctc gccggccggg
     4801 acctccaggt cgaggccgtc gagggccacc gtggagccgt aggtcttgac caggtcggtg
     4861 atcgagatcg cgattgccat ggcgggccct cccccccaga cggacggagc gatcgtactc
     4921 ccgcccgccg ggccccccaa cctccgccta cctcacgagg cgcgagaacc tccggtcggc
     4981 cagcaccttc cccccggtct ggcacctcgg gcagtacgtg acctcgtggg actcgtagga
     5041 gatccggcgg aggtcgtcgc cgcagcgcgg gcacggccgg ccccaccgcc cgtgcaccgc
     5101 gaagtgctcc ccgagcttcg ggggcaggcc gccgacgcgc ttccgctcct tctccaggcc
     5161 ctcggccaga acctcccgga cggcctcgga cagccgggcc cgttcctcgg cgtccagctt
     5221 cgccagcgag gcgaacggcg agagcttcgc ccggtggagg atgtcgtcgc tgtagccccg
     5281 gccgatcccg gccacggtcc gctgatcgcg gaggatcgtg tggacccgac gggtgtcctc
     5341 ggcttcccgg agccacgaga cgaacgcctc cgaggtcgcc tcgggcccga ggacggcgag
     5401 cggcccctcg tcgccgggag ccaggacgtg ccaagctgcg cgacgctcgg tcccgaactc
     5461 acggaccatg aggccgggcc gtcgggcgaa ccggagccgg acgaccccgc cccgaggcct
     5521 cgatcgcttg ggcgggtcct cgagggtcac gcggcctccc tgggagaggt gcagcgcgat
     5581 ccgcgggccg cccaggtcga acagcaacca cttcccgcgg cggccgacgc tctcgagtgg
     5641 ccgcccgatc agggactccg gcgacggctc gacggtcttc aacgccgaga actggatcgc
     5701 gtcgatgccc tcgagctcgg cgccgcggaa cacatcgtcg aggcgatcgg cgagggcctg
     5761 gacctctgga tactccggca cgcccccatg ctagcggggg ccggcgctca gccctccacg
     5821 tcctgctcgc ggagcaccag ctgctggcct tccgcggccg ggacggcgtc cacgaccgca
     5881 gccgagagcg gctccacgag ctcgggggcc aggaacacct ggatgtcctc cttctcggcg
     5941 accttgtgat cgccctcgat cgcctcgtcg gagaagccga accccaggcc gggctgctcc
     6001 tcctcgccgg aggcgacgag ctggatgcgg gccttcgcct cgtccgacac cccgggggtg
     6061 tccatcctga tctggcggat cacgccgacg gcggcttcgg tgacctcaag catgcgaaca
     6121 cacctcctcc tgccccaccc tatccgtcct cgggtcccgc caaccagggg acttgacgaa
     6181 ccgaccggtc ggtatgtaca gtccgcgcca tggttcgaac cgatcgcggc gaggagaccc
     6241 gccgccggat cctgacggag gccgccgcgg ccttcgccga gcgaggctac gccggcacct
     6301 cgatgaacga gctcgtgcga gcccccggcc tgaccaaggg cgccttctac ttccacttcg
     6361 actcccagga ggacctcgcg ctcgaggtct tccgctggaa gcacgaggag tggcaggcga
     6421 tggtcgccga ggcgatcgaa gagggggcgc gcgcggtcga tcagctcgtg gccgtgatgg
     6481 gcgcggtcac cgactgcgtc gacgccgatc cctcggctcg gtcgctgggc aggctcgccg
     6541 gcgagctttc cggggacccc aggctggcct cggtcctcga cgtgcagttc aagtcctggg
     6601 tcgagttctc gaccgagctg ctgcggcggg gtcaggagga gggggacgtg cggcacgacc
     6661 tcgatccccg ggaggtctcc gaggtcgtgg tggccgggtt catcgggctc gagcacgtct
     6721 cggggacgag aggtgcggga gatctccggc ggtggtcgga ggccctcacc gggttggtgc
     6781 tggatgcgat cagagccgag gagcaaggga catgaagggg atcggaaggt cggtgttcca
     6841 ccgcctgggc ggcttcgccg tccgccggcg gtggtgggtg gtggccgtgt gggtggcggc
     6901 gctggtggcc gcgttcgtgc cgctgcgcga gctggggggc cggctcagcc aaggagggtt
     6961 cgaggtgccc ggctccgagt cggagcgggt gcagctcgcg gtgcagagcg agttcacagg
     7021 gcagttcgag ttcagcgacc agctcgtgat gcggagcgag agtgtgcccg tggacgatcc
     7081 gcgattccgg gaggccttcg aggacgtcag ggcggccctc gtcgaggcgc cgggcgtggg
     7141 agacgtcacg gacccgtacg cgacgccgga gcgatcgatc ggccccgacg gccggacgct
     7201 cgtgtccacc gtcggcctca ccgacgacca ggaccaggcc ctggagcacg ccgacgagct
     7261 ccacgacgcc gtgcacggcg ccgtgccgca cggaggcgag atcgaggtct acctcaccgg
     7321 cgcggccccc ttctactcgg agttctcgga ggtgacccag cacgacctgg agcgggcgga
     7381 ggccatcgcc ctgccgatca gcctcctgat cctcatcgcc gcgttcgggt ccctggtcgc
     7441 ggcggggatg ccgctggccc tcgccctgtt cggcctggcg atctcgttcg ggatcatctc
     7501 gctcgtcgcc gccacgacga ccgtgagcat cttcacccag aacgtggcct cgatgatcgg
     7561 gatcggggtc ggcatcgact actcgctgtt cgtcgtgagc cgctaccgcc aggagctggg
     7621 gaagggccac gacgtgcccg aggccgtggc ccgagccatg gccaccagcg ggaaggccgt
     7681 catggtctcg gcgctcacgg tcgtgatcgc gctctcgggc acgctgctgg tggacatcgc
     7741 cgcgttccgg tcgatggggc tgggcgcgat gatcgcggtg gccgtcgccg gcctggccgc
     7801 gctcacgatc ctcccggccc tgctgggctt cgtggggagg aagatcaaca gcctgcgcct
     7861 gggtcggcgg cgcctgaagg acgaggagcg gtcgggcggc ctgtggcacc ggtgggcgat
     7921 gctcgtgatg cgacgcccct ggttcgccct gatcgggtcg ctggccctgc tggccctgct
     7981 ggcgctgccg gcccggaacc tgcgcctggg gacctcgggg cccgacatct tcccgcccga
     8041 ctcggagccg cgggtggcgg ccgaggcggt ggccggggcg ttcggcgagg ggcaggccgc
     8101 cccgatgcag atcctgatcg agcaccccga gggcgtgctc gagagcgggt tcgaggacgt
     8161 ggccgaggtg gccggggcgc tccagagcga tccgggcatc gtgcaggtgg actcgatcgc
     8221 gacgctggct ccggtggcga gcgtggagga ggctcgcgcg gtggccgcgt cgcccgaagc
     8281 ggcccccgtg gtgcagacga tggtggggaa cgaggggaag gccacgcttc tgcgggtggt
     8341 caccgccgat gaccccgggt ccgacgaggt ccgcgagctc gtgcgccggc tccgcgagga
     8401 gctgcccgcg ctcctgcctg caggggcaga ggtggccgtc ggaggggatc cgggcctcac
     8461 cgtggacctc aacgacgaga tcgagggctc gatcatcccg gtcgtggcga tggtcctcac
     8521 gctctcgttc ttcctgctgc tgctcttctt ccgcagcatc ctcctgccgc tgaaggcgat
     8581 cctcatgaac ctggcctcgg tgctggcgac ctacggcatc gtcgtgttca tcttccagga
     8641 gggccacttc gaggatctac tggggttctc ggccaccggg cacatcgagt cgttcgtgcc
     8701 gctgttcctg ttcacgatcc tgttcgggct ctcgatggac tacgaggtct tcctgctggc
     8761 cagggtccgc gaggagtacc tccgcacggg ggacaacacc gaggcggtcg gatggggcct
     8821 cgagcacacg gcgaggatca tcacgagcgc ggcggcgatc atggtgacgg tcttcggggc
     8881 gttcgccttc gccagcctcg tgccgatcaa gtcgatgggg ttcgggctcg ctgcggcggt
     8941 cttcctcgac gcgacgctgg tccgggtcgt gctcgtcccc gccacgatgc gcctgatggg
     9001 cgactggaac tggtggctgc cgggatggct cgaccgcatc ctgcccaacg tgtcgctgga
     9061 gggcgccgaa ccggagcccg ctgggcccgc ggaggccccg gcgaccgcct aggggagcgg
     9121 gggggccgcc atgccccccg gccctcctcc gcccgaacct ctgcatcccg gtccggagtc
     9181 gatgcagctt ggacatgttt ccgagcgccc gcgtatcgtg ccggcatgac cgaccccgac
     9241 cgcctctcga tctcggaggt gagtcgcttc gtcgacgtgc cgatccccac gatccgctcg
     9301 tgggagcgcc gatacggggt accggcgccc gctcgcacgg gaggtggaca ccgccggtac
     9361 acccgggagg aggtcgacct gatccgggcc atgcgcgacg agatcgtgcg gggggcgagc
     9421 gcacgcgacg cggcgcggag gatccggctg gctcccgcgc ccgcggtcga gcccaccccc
     9481 catctcgaga cgctcctgga ccgcgtcctc gatctcgacg atgcggggat ccggtggatc
     9541 ctcgaccagg ccgcggacgg gctcgggatc gagcgggccg tggtggaggt ggccctgccc
     9601 gccctgcgag aggtgggcga tcgatgggcg gggggccgct gcgacgtggc ccaggagcat
     9661 ctggcgacga acgaggtccg gacctggatc gcgaggctcg gccgcactgc gccaccaccc
     9721 ttccgcccgg cccccatcct tctggcgacg gcgccggacg agacgcacgc gaccgggctc
     9781 gaggccttcg ctctcctcct ggcgcgccgc gggtggccga cggtcgtcct cggcgcccgc
     9841 acccccgtgg acagcctgct gcgcacggtg agcaaggtag gtccggcggc ggtggtgctg
     9901 tcctcgcagt ggacgcgagc tcggcgctcg gccctcgagg cgctcgaggc cgtggcagcc
     9961 acctcggtgc cggtgttcta cgcgggagcg gcgttcgccg tgtcgtcaaa gcgggagggg
    10021 gttcccggcg tctacctggg ggacgacctc ctgggctccc tggatgagct ggagcggacg
    10081 gtggcgtgag ggcgcccgcg cggggagtca tccccgcgcg ggcgccccgt cggcctcagg
    10141 ccgcggcgcg caggccgtgc cgctcggcgg ccgcgggcct cgcggtcgcc agcatcggca
    10201 gacgcacggc cagccagatc
//
LOCUS       DASUTW010000165         6468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_958594, whole genome shotgun sequence.
ACCESSION   DASUTW010000165 DASUTW010000000
VERSION     DASUTW010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958594"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VF058_05670"
     CDS             <1..952
                     /locus_tag="VF058_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX6207833.1"
                     /translation="ADELDQYTERLVEERELEEFTRGLFPTFGEVSAPMSMLRTAVSA
                     ASAYDPDGWDHSEEGIERKAVRLTSRIATMIATYHRIRSGEEPVPPDPKLPHAANFLL
                     MLTGEEPDQEAARALDVCLVLHADHTMNASTFAARVVASTLSDMHSAITAAIAALKGP
                     LHGGANERVMEMLIEIDQPEKADDHVRRLLSEGQRIMGFGHRVYKTEDPRATHLRRLS
                     RELGERAGDTRWYEISERIEKAVMDEKGLYPNVDFYAASVYHYLGIPIDLFTTVFAAS
                     RVAGWTAHVREQLSDNRLIRPESEYIGPRDQAYVPLEERR"
     gene            949..1413
                     /locus_tag="VF058_05675"
     CDS             949..1413
                     /locus_tag="VF058_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6207834.1"
                     /translation="MKISDIMTKAAVIDRPDDTLASAAAKMWEQQTGSLLVLEGDELV
                     GIITERDVLKAVAAGTDLEATRISEVMSKDLITVDPGTSLREAAGIMTEKWIRHLPVL
                     QGGRLVGIVSQRDLSGVLANALNEPEGLHRLIESSELVRDQRLKRIEAGDLD"
     gene            complement(1416..2330)
                     /locus_tag="VF058_05680"
     CDS             complement(1416..2330)
                     /locus_tag="VF058_05680"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX6207835.1"
                     /translation="MTLRSESRLERLLHDGVFAVTAEVVPPRGGDPEPVRRQARDLAG
                     YADAVNVTDNPSSSAHMSSVAGAALVAGEGLEPVVQVTVRDRNRLAITADLLGAWALG
                     ARNVLCLTGDPAAIGDHPDAVEVKDLDVLDLVRFVTTMRDEGRLLSGAAADPAPRYFV
                     GVADVPLAERYDPGRLEAKIDAGADFVQTQIVYDLDALAEWADRLRPRGIFERAFVLV
                     GVAPPRSLGSVRFMQEHLPGVVVPGSVIERLEGARDDAAAEGVRLTVEIVEGLRSIPG
                     IGGVHVMGLGREAAVRGVIEGAGLLPRP"
     gene            complement(2327..3190)
                     /locus_tag="VF058_05685"
     CDS             complement(2327..3190)
                     /locus_tag="VF058_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HEX6207836.1"
                     /translation="MTARIIDGKGMAAEIRSEVAARVEALAGHGVTPGLAAVLVGDDE
                     PSRIYVGAKQKACAEVGIHSERHELAQEASEEELLELIRRLNEDPAVHGILVQLPLPP
                     QISTLTVHETIAVEKDVDGLTPASVGRLVRGEATYLPATPFGIVEMLVRTGIPIEGQE
                     VVVVGRGALVGMPLSIMLAQKSERGNATVTMCHTRTRDLARHTRRADILVVAAGRPGT
                     VTGDMVKPGAVVIDVAVNRTDAGLVGDVDFESAAEVAGWITPVPGGVGPLTVAMLLVN
                     TVTAAERLAGG"
     gene            complement(3217..3939)
                     /locus_tag="VF058_05690"
     CDS             complement(3217..3939)
                     /locus_tag="VF058_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX6207837.1"
                     /translation="MIVAEAVEKWFGGFQALKGITTTVRRGEVVVILGPSGSGKSTFI
                     RTINRLERHDAGRIVVDGIELTDDIRNIERVRSEIGIVFQSFNLFPHMSVLHNVTLAP
                     RNVRRRPKKQAEEKAMQLLERVGIPEQADKYPAQLSGGQQQRVAIARALAMEPKIMLF
                     DEPTSALDPEMIKEVLDVMRELARSGMTMIVVSHEIGFAKEVGDRVLFFDDGFLVEEN
                     TPERFFSRPETDRGRLFLSQIL"
     gene            complement(4005..5258)
                     /locus_tag="VF058_05695"
     CDS             complement(4005..5258)
                     /locus_tag="VF058_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX6207838.1"
                     /translation="MATVDEQRLQMIAAEERRAVEVAPQARPATPREWLKENLFSTWR
                     NGLVTVITALFVGWALYQILRWILVTGDFQVVKANLASYMLARFPREEVWRVWTSLYL
                     VLALAGLSWGYSERHIAWTRRRMVEATVLVLGGILVLAYLLDTVLIWMLIAGAVAALF
                     VPMAAGRLLAHRYRRPLRRVVIAGWILIYPAILAILLLFDGPSSRTWGGVLLNVMAGS
                     VAIVASFPIGILLALGRRSSFPAIRTFCVGVIELIRGVPLITLLISGVFILPLLLPPQ
                     LQMPTVMRVIVMLTVFSAVYVAEIVRGGLQGVHHGQYEAARALGLSTTRMTALVILPQ
                     ALRNTIPALISHFISLWKDTSLLAIVAGLTDLLAAARRSAAGLEFIGDSKEALLGAAF
                     IFWAVAFTASRWSQRLERRVGVGER"
     gene            complement(5258..>6468)
                     /locus_tag="VF058_05700"
     CDS             complement(5258..>6468)
                     /locus_tag="VF058_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6207839.1"
                     /translation="SERTRAPPHTMSETAVVETVRPPLWRNVRVLRVAGQVVFILAVL
                     FVGREIWLNLVFGVERQGIDLSLSFLQERAGFGIKEGIEYSANDTVTRALLVAFVNTV
                     IVASAGIALATIFGLLIGIGRLSPNWLVRKVTQVYVEIFRNTPVLVIIIFFYFGVVLA
                     LPLIGGGGIFGLAYVSNRGAAFPWPRVSEGVGLWALLLVAAVVAATLVARWRTRFNER
                     TGDPHHRVLWWLGTFLGLGAVAYALTGGPVAIDVPDQVGRGYVGGIQISGELFGVLAG
                     LVIYTSAFIAEITRGSILAVEKGQKEAAEALGFSPFQQMRFVVLPQAMRIAIPPINSQ
                     YLNLTKNTSLGLAVAYPELVSVGSTIINQKGRFTQVLVIWMLSYLALSLLISFVMNVL
                     NRIVAYRGER"
BASE COUNT         1060 a         2347 c         2181 g          880 t
ORIGIN      
        1 ggccgacgag ctcgaccagt acaccgagcg actcgtcgag gagcgggagc tcgaggagtt
       61 cacgcgcggc ctcttcccca cgttcgggga ggtgtccgcg ccgatgtcga tgctccggac
      121 cgccgtctcg gccgcctcgg cctacgaccc cgacggctgg gaccactccg aggagggcat
      181 cgagcggaag gcggtcaggc tgacctcgcg gatcgccacg atgatcgcca cctaccaccg
      241 catcaggagc ggggaggagc cggtcccgcc cgatccgaag ctcccccacg cggccaactt
      301 cctgctgatg ctcacgggcg aggagcccga ccaggaggcc gcccgggccc tcgacgtctg
      361 cctcgtgctg cacgccgacc acacgatgaa cgcctccacg ttcgcggcca gggtcgtggc
      421 ctccaccctc tcggacatgc actcggcgat caccgccgcc atcgccgcgc tgaagggccc
      481 cctgcacgga ggggcgaacg agcgcgtgat ggagatgctg atcgagatcg atcagcccga
      541 gaaggccgac gaccacgtgc ggcggctgct gagcgagggc cagcggatca tgggcttcgg
      601 ccaccgcgtc tacaagaccg aggacccgag agccacccac ctgcggcggc tgtcgaggga
      661 gctcggcgag cgggccggcg acacgcgctg gtacgagatc tccgagcgca tcgagaaggc
      721 cgtgatggac gagaagggcc tctaccccaa cgtcgacttc tacgcggcgt ccgtctacca
      781 ctacctgggg atcccgatcg acttgttcac gacggtgttc gcggcgagcc gggtggccgg
      841 gtggacggcc cacgtgcgcg agcagctctc ggacaaccgg ctgatccgcc ccgagtccga
      901 gtacatcggc ccgcgcgacc aggcctacgt ccccctcgag gagcgccgat gaagatctcg
      961 gacatcatga cgaaggcggc ggtgatcgac cgccccgacg acacgctggc ctcggccgcc
     1021 gcgaagatgt gggagcagca gaccggctcg ctcctcgtgc tcgagggaga cgagctcgtg
     1081 ggcatcatca ccgagcggga cgtgctgaag gccgtggccg cagggacgga cctcgaggcc
     1141 acccggatct cggaggtcat gtcgaaggac ctcatcacgg tggaccccgg cacctcgctg
     1201 cgcgaggccg cggggatcat gaccgagaag tggatccgcc acctcccggt cctccaggga
     1261 gggaggctcg tggggatcgt ctcccagcgg gacctctccg gagtgctggc caacgcgctg
     1321 aacgagcccg agggcctgca ccgcctgatc gagtcgagcg agctcgtccg cgaccagcgc
     1381 ctgaagcgca tcgaggccgg cgacctcgac tgaggtcagg gccggggcag gaggccggcc
     1441 ccctcgatca ccccccggac ggcggcctca cggcccagcc ccatcacgtg gacccccccg
     1501 atccccggga tcgagcgcag gccttcgacg atctcgaccg tcagccgcac gccctcggcg
     1561 gccgcgtcgt cgcgggctcc ctccagccgc tcgatgacgg atcccggcac caccacgcct
     1621 gggaggtgct cctgcatgaa tcgcacggag cccaagctcc tcggcggggc cacccccacg
     1681 agcacgaacg cccgctcgaa gatcccgcgc gggcggaggc ggtcggccca ctccgccagc
     1741 gcgtcgaggt cgtagacgat ctgcgtctgg acgaagtccg cgcccgcgtc gatcttcgcc
     1801 tcgagccggc ccgggtcgta ccgctcggcc agcggcacgt cggcgacgcc cacgaagtat
     1861 cgcggagcgg gatccgccgc cgctcccgac agcagccgcc cctcgtcccg catcgtcgtc
     1921 acgaatcgca ccaggtccag gacgtcgaga tccttcacct cgacggcgtc gggatggtcg
     1981 ccgatggcgg ccgggtcccc cgtcaggcag aggacgttcc gcgcgccgag ggcccaagct
     2041 ccgagcaaat cggccgtgat cgccaggcgg ttccgatccc gcaccgtcac ctggaccaca
     2101 ggctcgaggc cctcgcccgc caccagcgcg gcccccgcca ccgacgacat gtgggccgaa
     2161 gagctcgggt tgtcggtcac gttgacggcg tcggcgtagc ccgcgaggtc cctggcctga
     2221 cggcggacgg gctccgggtc gcctccccga ggcggaacga cctcggccgt caccgcgaag
     2281 acgccgtcgt gcaggagccg ctcgagccgg ctctccgacc ggagggtcat ccgccggcga
     2341 gccgctccgc ggccgtgacg gtgttcacga gcagcatcgc cacggtcagc ggtcccacgc
     2401 cccccggcac cggcgtgatc cagcccgcga cctcggcggc gctctcgaag tccacgtccc
     2461 ccacgaggcc cgcgtccgtc cggttcaccg ccacgtcgat cacgacggcc cccggcttca
     2521 ccatgtcgcc cgtcaccgtc ccgggacggc cggccgccac gacgaggatg tcggcccgcc
     2581 gcgtgtggcg ggcgagatcg cgggtccgtg tgtggcacat cgtcacggtg gcgttcccgc
     2641 gctcggactt ctgagcgagc atgatcgaca gcggcatccc cacgagcgcc ccccgcccca
     2701 ccacgacgac ctcctgcccc tcgatcggga tgccggtgcg cacgagcatc tccacgatgc
     2761 cgaagggggt cgccggcagg taggtggcct cgcctcgcac cagcctcccc acgctggcgg
     2821 gggtgaggcc gtcgacgtcc ttctccacgg cgatcgtctc gtgcacggtc agggtggaga
     2881 tctggggcgg gagcggcaac tggacgagga tcccgtgcac cgccggatcc tcgttcaggc
     2941 ggcggatcag ctcgaggagc tcctcctcgc tggcctcctg cgcgagctcg tggcgctcgg
     3001 agtggatccc gacctcggcg cacgccttct gcttcgcccc gacgtagatg cgcgagggct
     3061 cgtcgtcgcc cacgagcacg gcggccagcc ccggggtgac gccgtggccg gccagggcct
     3121 cgacgcgggc ggccacctcc gagcggatct cggccgccat ccccttcccg tcgatgatgc
     3181 gcgcggtcat gccggcgccc atcctaccgg ctcgactcag aggatctggg agaggaagag
     3241 ccgcccgcgg tcggtctcgg gccggctgaa gaagcgctcg ggggtgttct cctcgaccag
     3301 gaacccgtcg tcgaagaaga gcacgcggtc ccccacctcc ttggcgaagc cgatctcgtg
     3361 gctgacgacg atcatcgtca tcccggagcg ggccagctcc ctcatcacgt cgaggacctc
     3421 cttgatcatc tccgggtcga gggcggaggt cggctcgtcg aacagcatga tcttcggctc
     3481 catggccagt gcccgggcga tcgccacccg ctgctgctgg ccaccggaga gctgggcggg
     3541 gtacttgtct gcctgttccg ggatgccgac ccgctcgagc agctgcatcg ccttctcctc
     3601 ggcctgcttc ttcggccgcc ggcgcacgtt ccgcggcgcg agcgtgacgt tgtgcagcac
     3661 gctcatgtgg gggaagaggt tgaacgactg gaacacgatc ccgatctcgg accggacccg
     3721 ttcgatgttc cggatgtcgt cggtcagctc gatcccgtcg acgacgatcc ggccggcatc
     3781 gtgccgctcc aggcggttga tcgtccggat gaacgtggac ttgcccgacc cggacggccc
     3841 gaggatcacg accacctcgc cgcgccggac ggtcgtggtg atgcccttga gagcctggaa
     3901 gccgccgaac catttctcga cggcctcggc cacgatcatc cggtcgtcgc cgtccccgat
     3961 caccctcgcc tcctcgagct ccatctcaac ccgctcgtcg tcggtcatcg ctcccccacc
     4021 cccacccgtc gctccaggcg ctggctccac cgcgacgccg tgaacgccac ggcccagaag
     4081 atgaaggccg cgcccagcag cgcctccttg ctgtccccga tgaactccag gcccgccgcc
     4141 gaccgccgcg ccgcggcgag gaggtccgtc aggcccgcca cgatggcgag cagcgaggtg
     4201 tccttccaca ggctgatgaa gtggctgatg agggcaggga tcgtgttgcg cagagcctgc
     4261 ggcaggatca cgagcgcggt catccgggtc gtggacagcc ccagggcccg tgccgcttcg
     4321 tactggccgt gatgcacgcc ctgcaggccg ccccgcacga tctcggcgac gtacacggcg
     4381 ctgaacacgg tcagcatgac gatcacgcgc atcaccgtcg gcatctgcag ctgaggcggc
     4441 aggagcagcg gcaggatgaa caccccggag atcagcaggg tgatgagcgg cacgccccgg
     4501 atgagctcga tcacgccgac gcagaacgtg cggatcgccg ggaagctgct ccggcgcccg
     4561 agggcaagca ggatgccgat cgggaaggag gccacgatcg ccacgctccc ggccatgacg
     4621 ttgagcagca cgccccccca cgtccggctg gacggcccgt cgaacagcag caggatcgcc
     4681 aggatcgccg ggtagatgag gatccacccc gcgatcacga cccgccggag tggacggcga
     4741 taccggtggg cgaggagtcg tcccgccgcc atcgggacga acagcgccgc cacagcgccg
     4801 gcgatcagca tccagatcag gaccgtgtcg aggaggtagg cgagcacgag gatccctccg
     4861 agcacgagga ccgtcgcctc caccatccgg cgccgcgtcc aggcgatgtg gcgctcggag
     4921 tagccccagc tcagcccggc cagggccagc accaggtaca gcgacgtcca caccctccag
     4981 acctcctcgc gggggaatct ggcgagcatg tacgaggcca gattggcctt gaccacctgg
     5041 aaatccccgg tcacgaggat ccatcgaagg atctggtaca gggcccagcc cacgaagagg
     5101 gcggtgatca ccgtgaccag cccgttgcgc caggtcgaga agaggttctc cttcagccac
     5161 tccctggggg tggccggccg ggcctgcgga gcgacctcga cggcgcgccg ctcctcggcc
     5221 gcgatcatct gaagccgctg ctcgtcgacg gtggccatca gcgctccccc cggtaggcga
     5281 cgatgcggtt cagcacgttc atgacgaagg agatcaggag gctgagagcg aggtagctca
     5341 gcatccagat caccagcacc tgggtgaacc ggcccttctg gttgatgatc gtggatccga
     5401 cggacacgag ctccggatac gccacggcga ggccgaggct ggtgttcttg gtcaggttca
     5461 ggtactggct gttgatgggg gggatcgcga tccgcatcgc ctgcggcagg accacgaacc
     5521 gcatctgctg gaagggcgag aaccccaggg cctccgcggc ttccttctga cccttctcca
     5581 cggccaggat gctgccgcgg gtgatctcgg cgatgaaggc ggaggtgtag atcacgaggc
     5641 cggcgagcac cccgaacagc tcgccggaga tctggatccc gccgacgtag ccccgcccca
     5701 cctggtcggg gacgtcgatc gcgaccggcc cgccggtgag cgcgtaggcc accgcgccga
     5761 gcccgaggaa cgtccccagc caccagagca cccgatggtg ggggtcgccg gtccgctcgt
     5821 tgaaccgcgt ccgccagcgc gccacgagcg tggcggccac gacggccgcc acgagcagga
     5881 gcgcccacag cccgacgcct tcagagacgc gcggccacgg gaaagctgcg ccgcggttcg
     5941 agacgtaggc gaggccgaag atgccgcccc cgccgatcag cggcagcgcg agcaccaccc
     6001 cgaagtagaa gaagatgatg atgacgagca ccggggtgtt gcggaagatc tcaacgtaga
     6061 cctgcgtcac cttgcggacc agccagttgg gcgagagccg cccgatcccg atcaagagcc
     6121 cgaagatggt ggcgagggcg atgccggccg acgccacgat caccgtgttc acgaaggcca
     6181 cgagcagcgc gcgggtcacg gtgtcgttcg ccgagtactc gatgccctcc ttgatgccga
     6241 accccgcccg ctcctggagg aacgagaggg acaggtcgat gccttgccgc tcgacgccga
     6301 acacgagatt gagccagatc tcccgcccca cgaacagcac ggccaggatg aagacgacct
     6361 ggccggccac acgcagcact cggacgttcc gccagagcgg cggccggacg gtctcgacga
     6421 ctgcggtctc gctcatggtg tggggcgggg cgcgggttcg ttccgaac
//
LOCUS       DASUTW010000166         3167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_828828, whole genome shotgun sequence.
ACCESSION   DASUTW010000166 DASUTW010000000
VERSION     DASUTW010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828828"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VF058_05705"
     CDS             <1..346
                     /locus_tag="VF058_05705"
                     /inference="COORDINATES: protein motif:HMM:NF027650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6207840.1"
                     /translation="YLYRKEREGAERPASRPPAAKPRAPQAKERRRAAARERERLREG
                     REAVRRVEAELERATAELRAIEERLADPGFYAPGGEEVGEAVRRHGELRERIAELEAA
                     WEEAVSALEASG"
     gene            382..2613
                     /locus_tag="VF058_05710"
     CDS             382..2613
                     /locus_tag="VF058_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6207841.1"
                     /translation="MDDVPGGVLEADPPRFSADEASAIAARLFGVEGPATPLASERDQ
                     GFLLGGGVLKISNAGERPEVLDMETAAALHVRRVDPDLPLAMPIPLVRTDPPAYRAEV
                     GGHLIRMFERLPGRASVPGAELGLDAVRAFGEVLARLGRALRGFFHPAAGRVILWDVQ
                     HALSLRHLLGEIEDEEARALADRALRRFEERALPAWPLLRAQVIHGDLSLDNALLDEE
                     GRVTGIVDLGDMSHTALLCDLSSALASVLGGREGDEAFRTARVLLDGYQRITPLEPEE
                     IELLPLLLEARLAATVVISAWRVRRYPQNAEYIAGWDEETWPLLRLLDEMGAEEAAAR
                     LGAPRAAPTEDLARRRRRLLGPALSPLTYRRPVHAARAEGVWIVETDGRRLLDAYNNV
                     PTVGHSHPRVAEAVARQSRLLATNARYLYEPLIELAERLVGTMPSALDTVTLVNSGTE
                     ANDLAWRLAVAWTDAPGAIVTAHAYYGVSAATADLSPETWREPSDHVETFGPPGEGAG
                     RAIERLAARGLRPAALFLDGGFTSDGILVPGSPYVRDLAREIQSAGGLFVADEVQVGH
                     GRTGEHLWSFAADGVTPDVVTMGKPMGNGFPVAAVVTRREIAARLAESTEVFSTFGGN
                     PVAAAAALAVLDVIADEGLIERAAEVGGVLLEELRAMARRHDEVRDVRGRGLLIGVEL
                     ASPAAPVADAMREAGVLVGVTGPRGEVLKIRPPLVFGPHHAARLVETLDRILGLRDPT
                     PGE"
     gene            2661..3101
                     /locus_tag="VF058_05715"
     CDS             2661..3101
                     /locus_tag="VF058_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX6207842.1"
                     /translation="MDALKLLKEDHEKVKKILDELESTTERGVKTREDLFQRLRRELN
                     VHEAIEEEILYPALKEHPKTKELALEAYEEHHVVDMVMEELEGVPFDDETWGAKLMVA
                     KENLEHHIEEEEGEMFQQARQVFDDEELGELGDRMEARKSELLG"
BASE COUNT          421 a         1088 c         1250 g          408 t
ORIGIN      
        1 ctacctctac cggaaggagc gggagggggc ggagaggccc gcctcgcggc cgcccgcggc
       61 gaagcccagg gcaccccagg cgaaggagcg gcggcgggct gcggcccgcg agcgggagcg
      121 tctgcgcgag gggcgggagg ccgtgcggcg ggtggaggcc gagctggagc gggccacggc
      181 cgagctccgg gcgatcgagg agcggttggc cgacccgggc ttctacgccc ccgggggcga
      241 ggaggtgggc gaggccgtcc gtcggcacgg ggagctgcgc gagcggatcg ccgagctgga
      301 ggccgcgtgg gaggaggcgg tctcggccct cgaggcctcg ggctgaggcg cgggccgcgg
      361 ttccgcgcta ccgtgccggc cgtggacgac gttcccggag gggtcctcga ggccgacccg
      421 ccgaggttct cggccgacga ggcctccgcg atcgcggctc ggctcttcgg tgtggagggg
      481 ccggcgacgc cgctggccag cgagagggac cagggcttcc tgctgggcgg aggcgtcctg
      541 aagatctcga acgccggaga gcggcccgag gtcctcgaca tggagacggc cgcggcgctg
      601 cacgtccggc gggtggaccc cgacctgccc ctggccatgc cgatcccgct ggtgcggacc
      661 gacccgcctg cgtaccgtgc cgaggtcggc gggcacctca tccggatgtt cgagcggctg
      721 ccggggcgcg cctcggtgcc gggggcggag ctcggcctcg atgccgtgcg ggccttcggc
      781 gaggtgctgg cccggctggg gcgcgccctg cgcggcttct tccacccggc ggcggggcgg
      841 gtcatcctgt gggacgtcca gcacgcgctc tccctccgtc acctcctggg tgagatcgag
      901 gacgaggagg ctcgggcgct ggccgaccgg gcgctgcgtc ggttcgagga gcgagccctc
      961 ccggcgtggc ctctccttcg ggcccaggtg atccacggcg acctctcgct cgacaacgcg
     1021 ctcctcgacg aggagggccg cgtgaccggg atcgtggacc taggggacat gagccacacc
     1081 gcgctcctgt gtgacctgtc gtcggcgctg gcttcggtcc tgggcggccg cgagggggac
     1141 gaggccttca ggaccgcgcg cgtgctgctg gacggctacc agcggatcac tcccctggag
     1201 cctgaggaga tcgagctgct gcccctcctg ctcgaggcga ggctggcggc caccgtggtc
     1261 atcagcgcct ggcgcgttcg acggtacccg cagaacgccg agtacatagc gggctgggac
     1321 gaggagacgt ggccgctgct gcggctgctg gacgagatgg gggccgagga ggcggcggcg
     1381 cggctgggag cgccgcgggc cgccccgacc gaggacctcg ccaggcgccg ccgccgcctc
     1441 ctcggccccg cgctctcgcc gctcacctac cgccgcccgg tccacgccgc ccgggccgag
     1501 ggggtttgga tcgtcgagac cgacgggcgg cggctcctcg acgcctacaa caacgtcccg
     1561 accgtggggc actcgcaccc gagggtggcc gaggccgtgg cccggcagtc gcgcctgctg
     1621 gcgacgaacg cccgctacct ctacgagccg ctgatcgagc tcgccgagcg tctcgtgggc
     1681 acgatgccgt cggccctcga caccgtgacc ctcgtgaact cggggaccga ggccaacgac
     1741 ctggcctggc ggttggcggt ggcctggacc gacgccccgg gcgcgatcgt caccgcgcac
     1801 gcctactacg gggtgagcgc ggccacggcc gatctctccc cggagacgtg gagggagccg
     1861 tccgatcacg tggagacctt cggtcccccg ggggaggggg ccggccgggc gatcgagcgg
     1921 ctggccgccc gcggcctgag gcccgccgcc ctcttcctgg acgggggctt caccagcgac
     1981 gggatcctcg tcccgggatc cccgtacgtc cgggacttgg cccgggagat ccagagcgcg
     2041 ggcgggctct tcgtcgccga cgaggttcag gtcggccacg gccgcaccgg cgagcacctc
     2101 tggtcgttcg ccgccgacgg cgtgacgccg gacgtcgtca cgatgggcaa gccgatgggg
     2161 aacgggttcc cggtggcggc ggtggtcacg cgccgcgaga tcgccgccag gctcgccgag
     2221 tcgacggagg tcttctccac gttcggcggg aacccggtcg ccgcggccgc agccctggcc
     2281 gtgcttgacg tgatcgccga cgagggcctg atcgagcgcg cggcggaggt ggggggcgtg
     2341 ctgctcgagg agctccgtgc gatggcccgc cgtcacgatg aggtgcgcga cgtcaggggg
     2401 cgggggctcc tgatcggcgt cgagctggcc tcgcccgccg caccggtcgc cgacgcgatg
     2461 cgcgaggccg gggtgctcgt cggggtgacg gggccgcggg gtgaggtcct caagatccgg
     2521 cccccgctcg tcttcggccc tcatcacgcc gccaggctgg tggagaccct ggaccggatc
     2581 ctggggcttc gggacccgac gcccggggaa tgatgcgctg gcggcgcgcc gggcgccgcc
     2641 acggacccgg gaggtgccgg atggacgcgc tgaagctgtt gaaggaagac cacgagaagg
     2701 tcaagaagat cctggacgag ctcgagtcga cgaccgagcg aggcgtgaag acccgcgagg
     2761 acctcttcca gaggctgcgc cgcgagctca acgtccacga ggcgatcgag gaggagatcc
     2821 tctaccccgc cctcaaggag caccccaaga cgaaggagct cgccctcgag gcctacgagg
     2881 agcaccacgt cgtggacatg gtgatggagg agctcgaggg cgttcccttc gacgacgaga
     2941 cctggggcgc gaagctcatg gtggccaagg agaacctcga gcatcacatc gaggaggaag
     3001 agggcgagat gttccagcag gcccgccagg tcttcgacga cgaggagctg ggcgagctgg
     3061 gcgaccggat ggaggcccgc aagagcgagc tgctgggctg aggccggtcg ggcccggcta
     3121 tcctcgggtc cgatgccacg cttcctcacc cccaggtggc tggtcgc
//
LOCUS       DASUTW010000167        10315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1216904, whole genome shotgun sequence.
ACCESSION   DASUTW010000167 DASUTW010000000
VERSION     DASUTW010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10315
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216904"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="VF058_05720"
     CDS             <1..1046
                     /locus_tag="VF058_05720"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX6207843.1"
                     /translation="AMAARAPLRDRATLALVFSTPHHEPAEVLDAVREAASPEAVVGC
                     VGQAVVAGPREVEDGPAVAVWLGRLPDRVEPYAVTFDGERFVGMPEGSGTHLVIADPF
                     TFPADAFLDDLEERRPGTAVIGGMASGVYPPGSTRLFMNDEVVTTGAVGARLPGGVAV
                     RALVSQGCRPVGPPFTVTRAEGSLIEELGGRSPLDRLREIYAAADERDRGLLADGLHL
                     GRVVDEHAEEGDFLIRGVLGADRDTGAMAVGDRVRVGETVRFHVRDADSADDDLRRAL
                     AGVDRPRGALLFTCNGRGRRLFAGPDHDAALVAEELGEPPVAGFFAAGELGPVGGRSF
                     LHGFTASLALFYG"
     gene            complement(1050..1490)
                     /locus_tag="VF058_05725"
     CDS             complement(1050..1490)
                     /locus_tag="VF058_05725"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX6207844.1"
                     /translation="MSAPPDLHPTVAQELRQGGQRYTPVRRRLVGILVEADGPLTIPE
                     ILRRGPRLAQSSVYRNLAVLERAGVVHRVVTSEEHARYELAEELSGHHHHLICASCGR
                     VEDFVTPRRVEQVLDRTMSEVAEGAGFSPTHHRLDLVGLCDRCS"
     gene            complement(1487..2365)
                     /locus_tag="VF058_05730"
     CDS             complement(1487..2365)
                     /locus_tag="VF058_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX6207845.1"
                     /translation="MTLPWPFEREYMQLALAAGLVVGACAPLIGAFLVQKRMSLMGDG
                     LGHLAFAGVAGAILLGVWPLWGALLFTVVGALAVEGLRARGKTSGDLALAIAFYVGIA
                     SGVVLLGLAGSLNATVFSYLFGQILTVTGGEVAVVAGLGAVIVLAFALSRRALFATVG
                     DEEWAQVAGLPVGALNALLAVLTAIIVVAAMRVVGLLLVAALMVLPVAGGQLLARSFG
                     GTLLWSSGIGVGSVVAGLIAARALGLAPSGTIVLLAAAAFLAAAFVSWIRGRLGGTGE
                     TASLHFEERVSGGEDA"
     gene            complement(2362..3111)
                     /locus_tag="VF058_05735"
     CDS             complement(2362..3111)
                     /locus_tag="VF058_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX6207846.1"
                     /translation="MSTDELARLEGVSFSFDGSPILSDVDLRVGRGEFVALVGPNGSG
                     KSTLLRILLGLLPARGTVRLLGRVPNELRERWRIGYVPQRPMLAGDQVASVEEVVAAG
                     RLARRGWWRRLRGEDRDVIGHALESVGLLPLRHRRVSHLSVGQQQRVFIARAFASEPE
                     LLVLDEPISGVDAESQRRFRESLLHTIEDHGTGVLLVSHELGAVAEDLDRVVVLKGRV
                     LFDGPPSDLMARGVSLGVHPEDLPFWLEELR"
     gene            complement(3108..3968)
                     /locus_tag="VF058_05740"
     CDS             complement(3108..3968)
                     /locus_tag="VF058_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX6207847.1"
                     /translation="MTISAAVLVAGCGAEAADGVGSVEVAAGFFPLAEAVAIAGGERV
                     HVENLTAAGSDPHDVELGTDEVDTILDADLLLYLGGFQPGVEELAGRRDGPSIDLLRG
                     VDTLSEGGEVDPHVWLDPLRYATVVERIGRALADVDPQGADAYLSDAEAHADEIRALD
                     ARFERRLAACEDDLLVVSHAAFGYLADRYGLRQEAISGISPESEPDPQRLAELIDVVR
                     TEGVTTVFTETLASPEVSRTLAREAGVRTAVLNPLEGLTPEEEAAGETYLSVMERNLV
                     TIAEGLGCPE"
     gene            4083..6719
                     /locus_tag="VF058_05745"
     CDS             4083..6719
                     /locus_tag="VF058_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6207848.1"
                     /translation="MRELPTGTITFLLTDIEGSTRILARLRDRYPALLEEHQRLLREV
                     FSTAGGVEVGTEGDSFFVVFPAAPNAVAAAAKAQQALESHEWPGGERVRVRMGLHTGE
                     AILGGDNYTGIDLHRAARIAAAAHGGQVLLSEATHALVAQTLPEGVTIRDLGEHRLKD
                     LPRAERLYQLQIPDLEQDFPPIRSLETRRGNLPEPLTSFVGRLQELAEIHDILAETRL
                     LTLTGPGGSGKTRLSIQVGAGLQDDFEGGAYFVPLAALEEHELVVPTIAETLGVEEDP
                     GRPSIETLIEHLANREVLLILDNFEQVTPAASTVGEILGATDRVKVLATSREPLHLAG
                     EREYPVPPLELPDPSHPPPLEALPRYEAVQLFIQRASAVQPSFTVTEENASAVVEICA
                     RLDGLPLAIELAAARVKLLSPEELLKRLDQRLSVLASSARDLPERQRTLRGAIAWSYD
                     LLDDRERGFFRRASVFMGGFTLESAEEVCNSDGELGDTLELVASLLDKSLIRPFSGPA
                     GSRYQMLQTIGEYAKEMLESSGEDEEVGRRHAAHVLRTVEAALPRLFGPERALWLDRL
                     AAEHDNLRAALTWTLEQGALETGLTLGGKAWRFWQMRGHLREGREWLGRLLAHPSAAQ
                     HMAPRADALEGLGGILYWMGDPEAAAVYDECLRLRREIGDPATIAEALYNRGSMNVFG
                     IGAPQDSGAALPLLEEALELFRELGDDTGVAKVQWAVGSAHLHDEDYEAAEEALSESI
                     ALYRAAQDLFGEAWGLHMLAVTLLHGGRGEDALPHLRRAVDIFDEAGDRSAIPVLLYD
                     AAIYAYQVGERDRAVLLMGVAEKVQQETGVGLGEASVEFVGLLTPLIEAMTDEERERG
                     EIAARDVTEEQAVDMIRQLCSG"
     gene            6758..7861
                     /gene="hppD"
                     /locus_tag="VF058_05750"
     CDS             6758..7861
                     /gene="hppD"
                     /locus_tag="VF058_05750"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX6207849.1"
                     /translation="MAQQEDFLPLMGIDHVEFYVGNARQAAHYYSKAFGFEVTAYRGP
                     ETKHRDTVSWLCEQGEVRFVFTGAQGPEHPIAKHVFEHGDGVKDIALRVPDAASAWEE
                     TTKRGAESAMEPEVFEDEHGKVVRSAIKAYGETIHSFVERENYSGPFLPGYRREEPYP
                     DEGPEVGIKAIDHCVANVELGKMNEWVRFYAEVMGFAQLMHFRDDQISTEYTALMSKV
                     MWDGSGRVKFPINEPAEGKKKSQIEEYLDYYRSPGVQHIAMQTDDIVRTVTALRERGV
                     RFIRVPETYYQDLAERVGEIEEDYSDLQKLAILADRDEEGYLLQIFTKIQQDRPTVFY
                     EFIERHGSRGFGLGNFKELFIAIEREQAERGNL"
     gene            7957..8475
                     /locus_tag="VF058_05755"
     CDS             7957..8475
                     /locus_tag="VF058_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6207850.1"
                     /translation="MRILVRHVMTRDVASVSASAGCGDIARLMVDREVTTIPVVDHDG
                     RLEGLVTSSGLLEHVGGKREPLHTLAVLATPGRHPHPLPCELRAVDVMCREPAVTRRE
                     ATVPEAIETLVTSRARALPVVDDDGRLLGIIGRSDLLRGLVPMIRSVDGVVGLHPAHG
                     RRHARVERLRAS"
     gene            complement(8476..9069)
                     /locus_tag="VF058_05760"
     CDS             complement(8476..9069)
                     /locus_tag="VF058_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX6207851.1"
                     /translation="MAQRRRMRAPERRQQLLDVARKVFGRNGFHTVTMDTVAKEAGVT
                     KPILYDHFSSKRDLYLALLEADLASLKQAVHTALETSRGNRERIRASFQAYFDFVDEH
                     AEGFRLLMSEAVGSERDFREMVERFREDVHAEVTEVIHRESRGKVPMEDARDVALGLI
                     GMAETAAQANPGASKDDRRRTVDALVRLAWRGITGLA"
     gene            9158..>10315
                     /locus_tag="VF058_05765"
     CDS             9158..>10315
                     /locus_tag="VF058_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6207852.1"
                     /translation="MEFRLNEDQLTAQKWAREFAEKEIRPVAPEYDESEEFPWPVLKK
                     AAEIGLYSLEFYVEMAGADPTGLIMPIVLEESCWGCAGISLGIFGTGLPLATLAGTGT
                     PDQFLEWAPRMLGTSEEPKIGAFAVTESGAGSDVSALRTTAKRDGDEWVLNGQKIFIT
                     NGGIADVHIVVATVDPELGHRGQAAFIVGPDTPGISQGKKERKLGIRASHTAEVLLDD
                     CRVPLENVGGGMERLEAKLARARENGSEPSRSSQALKTFELTRPLVAAQALGIARAAL
                     EFSVDYAKERTTFGRPIIEHQGIGFKLADMAMEVDAARLLTWRAAWMVKNDQLERAEG
                     SMSKLKASEVAVWVTEQAIQVLGGYGYIKDFPVEKWYRDAKIYTLFEGTSEI"
BASE COUNT         1559 a         3660 c         3696 g         1400 t
ORIGIN      
        1 ccgcgatggc cgcgcgcgct cccctccgcg atcgggcgac gctggcgctc gtcttctcca
       61 cgccccatca cgagccggcc gaggtgctcg acgcggtccg ggaggcggcg tccccggagg
      121 ccgtcgtggg gtgcgtcggc caggccgtcg tggcgggccc gcgggaggtc gaggacggcc
      181 ccgcggtggc cgtgtggcta ggccgcctcc cggaccgcgt cgagccgtac gcggtgacct
      241 tcgacgggga acgcttcgtc gggatgccgg agggctccgg aacccacctc gtgatcgccg
      301 accccttcac gttcccggcc gacgcgttcc tcgacgacct cgaggagcga cgcccgggca
      361 ccgcggtgat cggcgggatg gccagcggcg tctaccctcc cggctccacc cggctcttca
      421 tgaacgatga ggtcgtgacc acaggcgccg tcggggccag gctcccgggc ggtgtggcgg
      481 tccgcgccct cgtctcccag gggtgccgtc cggtggggcc gccgttcacc gtcacgaggg
      541 ccgaggggag cctgatcgag gagctcgggg gccgctcgcc cctcgatcgg ctccgcgaga
      601 tctacgccgc cgccgacgag cgagaccgcg gcctcctggc ggacgggctg cacctgggcc
      661 gggtcgtgga tgagcacgcg gaggaggggg acttcctgat ccgaggtgtg ctgggggccg
      721 accgcgacac gggcgcgatg gcggtgggcg accgggtgcg cgtcggggag acggtgcggt
      781 tccacgtgcg cgacgccgac tcggccgacg acgatctccg cagggccctc gcgggggtcg
      841 accggccgcg cggcgccctc ctgttcacct gcaacggtcg ggggcggagg ctgttcgccg
      901 ggcccgacca cgacgcagcg ctcgtggccg aggagctcgg ggagccgccg gtggccggct
      961 tcttcgccgc gggggagctg ggaccggtcg ggggccggag cttcctccac ggcttcacgg
     1021 cgagcctggc gctcttctac gggtagcgct cagctgcagc ggtcgcagag gcccaccagg
     1081 tcgagcctgt ggtgcgtcgg agagaacccc gccccctcgg ccacctcgga catcgtccgg
     1141 tcgagcacct gctccacgcg ccgcggcgtg acgaagtcct ccacgcgccc gcacgaggcg
     1201 cagatcaggt ggtggtggtg gccggagagc tcctcggcga gctcgtaccg cgcgtgctcc
     1261 tcgctcgtca cgacccggtg caccaccccg gcccgctcga gcacggcgag gttccggtac
     1321 acggagctct gggcgagcct cggtccccgg cgcaggatct cggggatcgt gaggggtccg
     1381 tcggcctcga cgaggatccc cacgaggcgc cggcgcacgg gcgtgtagcg ctggccgccc
     1441 tggcggagct cctgcgccac ggtcgggtgg aggtcgggcg gggcgctcac gcgtcctccc
     1501 ctccggacac gcgctcctcg aagtggaggg aggcggtctc gcccgtccct ccgagccgcc
     1561 ctcggatcca cgagacgaac gccgcggcga ggaaggccgc cgcggcgagg aggacgatcg
     1621 tcccgctcgg ggccagcccc agcgccctcg ccgcgatcag cccggcgacc accgagccca
     1681 ccccgatccc cgacgaccag aggagcgttc ccccgaacga gcgggccagc agctggcccc
     1741 ccgccacggg gaggaccatc agcgccgcga cgagcaggag cccgacgact cgcatcgcgg
     1801 ccacgacgat gatcgcggtc aggacggcga ggagcgcgtt gagggcgccg accgggaggc
     1861 ccgcgacctg agcccactcc tcgtcgccga ccgtggcgaa gagggcgcgc cgggagagcg
     1921 cgaacgccag gacgatgacc gccccgaggc ccgccacgac ggcgacctcc ccgcccgtga
     1981 cggtcaggat ctgcccgaag aggtagctga agacggtcgc gttcagcgag cccgccagcc
     2041 cgagcagcac cacgccggac gcgatcccca cgtagaaagc gatcgccagc gccaggtccc
     2101 ccgacgtctt cccccgggct cgaagcccct ccacggcgag ggcgcccacc accgtgaaca
     2161 gcagcgcccc ccacagcggc cacacgccga ggaggatggc cccggccacg cccgcgaacg
     2221 cgaggtgccc caggccatcc cccatgagcg acatccgctt ctggaccagg aacgcgccga
     2281 tcagcggggc gcaggcgcct acgacgaggc cggcggccag cgcgagctgc atgtactccc
     2341 gctcgaaggg ccaggggagg gtcatcgcag ctcctccagc cagaagggga ggtcctcggg
     2401 gtgcaccccc aggctcactc cccgggccat caggtcggag ggcgggccat cgaagagcac
     2461 ccggcccttc agcacgacga cgcgatccag gtcctcggcc acggccccca gctcgtggga
     2521 gacgaggagc acccccgtcc cgtggtcctc gatcgtgtgg aggagcgatt cgcggaagcg
     2581 ccgctgggac tcggcgtcca cgcctgagat cggctcgtcg agcacgagca gctccggctc
     2641 ggaggcgaac gccctggcga tgaacacccg ctgctgctgt cccacggaca gatggctcac
     2701 gcgtcgatgg cgcaggggga gcaggcccac cgactcgagc gcgtgcccga tcacgtcccg
     2761 gtcctccccc cgcaggcggc gccaccaccc gcggcgggcg agccggccgg ccgcgaccac
     2821 ctcctcgacg ctggccacct gatccccggc gagcatcggc cgctggggca cgtacccgat
     2881 ccgccagcgc tcgcggagct cgttcggcac gcgccccagc agccggaccg tccccctcgc
     2941 ggggagcagg ccgagcagga tgcggaggag ggtcgacttg ccgctgccgt tgggccccac
     3001 gagcgccacg aactcgcccc gcccgacccg gaggtccacg tcagagagga tgggggagcc
     3061 gtcgaacgag aacgagacgc cttccagcct cgccagctcg tccgtactca ctccggacat
     3121 ccgaggccct ccgcgatcgt caccaggttg cgctccatca ccgagaggta ggtctcgccg
     3181 gcggcctcct cctccggggt caggccctcg agggggttca ggacggccgt ccggacgccg
     3241 gcctcccggg ccagcgtccg ggagacctcc ggtgacgcca gggtctcggt gaagaccgtc
     3301 gtgacccctt cggtccggac gacgtcgatc agttccgcca gccgctgagg atcgggttcg
     3361 ctctccggcg agatccccga gatggcctcc tgccggagcc cgtaccggtc cgcgaggtag
     3421 ccgaaggcgg cgtgcgagac gacgaggagg tcgtcctcgc acgccgccag gcgccgctcg
     3481 aagcgcgcat cgagcgcacg gatctcgtcg gcgtgagcct ccgcgtcgct caggtaggcg
     3541 tccgcccctt gggggtcgac gtcggccagc gcccggccga tccgctccac caccgtggcg
     3601 taccggagcg gatcgagcca gacgtggggg tcgacctctc cgccctcgga gagcgtgtcc
     3661 acgccccgaa ggaggtcgat ggaggggccg tccctccgcc cggcgagctc ttccaccccc
     3721 ggctggaagc cacccaggta caggaggagg tcggcgtcga ggatcgtgtc cacctcgtcg
     3781 gtgcccagct ccacgtcgtg ggggtcgctt ccggcggctg tgaggttctc gacgtggacg
     3841 cgctcgccgc ccgcgatcgc gaccgcctcg gcgagaggga agaagcccgc cgcgacctcg
     3901 acgcttccga cgccgtcggc cgcctcggcc ccgcatccgg ccacgagcac cgccgcgctg
     3961 atggtcatga gaatcatcct cattatcaag ggatggactc tagcagggac gtcccttccg
     4021 ccgcaaccct gtgagcggca cgaacggggg cgcataatgg gcgcgccgat cgagggagga
     4081 cgatgcgcga gcttccgacc gggacgatca cgttcctgct cacggacatc gaggggagca
     4141 cacggatcct ggcccgcctc agggaccgct accccgcgct gctcgaggag caccagcggc
     4201 tcctccggga ggtcttctcc acggccggcg gggtggaggt gggcaccgag ggcgactcct
     4261 tcttcgtggt gttcccggct gccccgaacg ctgtggccgc cgccgcgaag gctcagcagg
     4321 ccctcgagag ccacgagtgg ccgggagggg agcgggtccg cgtgcggatg gggctgcata
     4381 ccggagaggc gatcctgggc ggcgacaact atacgggcat cgacctgcac cgcgcggcgc
     4441 gcatcgcggc ggccgcgcac gggggccagg tcctgctgtc cgaggcgacg cacgccctcg
     4501 tggcccagac gctgcccgag ggcgtgacga tccgagacct cggggagcac cgcttgaagg
     4561 atctccccag ggcggagcgc ctctaccagc ttcagatacc cgacctggag caggacttcc
     4621 cgccgatccg ctcgctggag acccggagag gcaacctgcc cgagccgctc accagcttcg
     4681 tcggccggct ccaggagctc gcggagatcc acgacatcct ggccgagacc cgtctgctca
     4741 ccctgaccgg cccgggcggg tccgggaaga cccgcctctc gatccaggtc ggcgccggac
     4801 ttcaggacga cttcgaaggg ggcgcctact tcgtgccgct ggcggcgctc gaggagcacg
     4861 agctggtcgt ccccacgatc gccgaaacgc tgggggtcga ggaggatccc ggtcggccat
     4921 cgatcgagac cctcatcgag cacctcgcca atcgggaggt gctcctgatc ctcgacaact
     4981 tcgagcaggt cacccccgcc gcctccaccg tgggcgagat cctcggggcc accgaccggg
     5041 tcaaggtgct ggccaccagc cgcgagccgc tccatctcgc cggggagcgc gagtaccccg
     5101 tgccgcccct ggagctcccc gacccgtcac accctccacc gctcgaggcg ctgccgcggt
     5161 acgaggccgt ccagctcttc atccagcgcg cctccgccgt acagccgagc ttcaccgtga
     5221 cggaggagaa cgcgtcggcc gtggtcgaga tctgcgcgcg tctcgacggg ctgcccctcg
     5281 ccatcgagct ggcggcggcg cgcgtcaagc ttctctcgcc cgaggagctc ctcaagcgcc
     5341 tcgaccagcg cctctccgtg ctcgccagct ccgcccggga cctcccggag cgtcagcgca
     5401 ccctgcgggg ggccatcgcg tggagctacg acctcctgga cgaccgggag cgcgggttct
     5461 tccgtcgggc ctcggtcttc atgggaggct tcacgctcga gtcggccgag gaggtgtgca
     5521 attcggacgg cgagctgggg gacaccctgg agctcgtggc ctcccttctc gacaagagcc
     5581 tcatccgacc gttctccggc ccggcaggca gccgctacca gatgctccag acgatcggcg
     5641 aatacgcgaa ggagatgctg gagagctcgg gcgaggacga ggaggtcggg cgccgccacg
     5701 cggcccacgt cctgcgaacg gtggaggcgg ccctgccgag gctcttcggc cccgagcggg
     5761 ctctttggct ggaccgcctc gcggcggagc acgacaacct ccgcgccgcg ctgacgtgga
     5821 ccctggagca gggggccctc gagacgggcc tcacgctggg ggggaaagcc tggcgcttct
     5881 ggcagatgcg gggccacctc cgcgaaggtc gggaatggct cgggaggctc ctcgcccatc
     5941 cctcggccgc ccagcacatg gctccccggg cggacgccct ggagggcctg ggtgggatcc
     6001 tctactggat gggggacccg gaggcggccg ccgtctacga cgagtgcctg aggctccgcc
     6061 gggagatcgg cgacccggcg acgatcgcgg aggcgctcta caaccggggg agcatgaacg
     6121 tcttcggcat cggagctccc caggactccg gcgccgcgct gccgctgctc gaggaggccc
     6181 tcgagctctt ccgggagctg ggagacgaca caggggtggc gaaggtgcag tgggcggtgg
     6241 gcagcgccca cctccacgac gaggactacg aggccgcgga ggaggccctg tcggagagca
     6301 tcgccctcta tcgagccgcg caggacctgt tcggggaggc gtggggcctg cacatgctcg
     6361 cggtgaccct gctccacggg gggcggggcg aggacgccct gccccacctg cggcgcgccg
     6421 tcgacatctt cgacgaggcg ggcgaccgat cggcgatccc ggtgctcctc tacgatgccg
     6481 cgatctacgc gtatcaggtc ggcgagcggg atcgagcggt gctcctgatg ggcgtcgccg
     6541 agaaggtgca gcaggagacc ggcgtcggcc tgggggaggc gtccgtcgag ttcgtcgggc
     6601 tcctcacgcc cctgatcgag gccatgacgg acgaggagcg cgagcggggc gagatcgcgg
     6661 cgcgcgatgt caccgaggag caggccgtcg acatgatcag gcagctgtgc tcggggtagt
     6721 tgctagagtg acacgacgga tccaggaggt gtggagcatg gcccagcagg aagacttcct
     6781 ccccctgatg gggatcgatc acgtcgagtt ctacgtcggc aacgcgcgcc aggccgccca
     6841 ctactactcc aaggccttcg gcttcgaggt caccgcctac cgcggtcccg agacgaagca
     6901 ccgcgacacg gtctcctggc tctgcgagca gggagaggtg cgcttcgtct tcacaggggc
     6961 ccagggcccg gagcatccca tcgccaagca cgtcttcgag cacggagacg gcgtcaagga
     7021 catcgccctg cgcgtgcccg atgccgcctc ggcctgggag gagaccacca agcgcggcgc
     7081 cgagagcgcg atggagcccg aggtcttcga ggacgagcac ggcaaggtcg tgcgctcggc
     7141 gatcaaggcc tacggcgaga cgatccactc gttcgtggag cgcgagaact actcgggccc
     7201 gttcctcccc ggctaccgcc gggaggagcc ttaccccgac gagggccccg aggtcggcat
     7261 caaggcgatc gaccactgcg tggccaacgt cgagctcggc aagatgaacg agtgggtgcg
     7321 cttctacgcc gaggtcatgg ggtttgccca gctcatgcac ttccgcgacg accagatctc
     7381 gaccgagtac accgcgctga tgtcgaaggt gatgtgggac ggctcgggcc gggtcaagtt
     7441 ccccatcaac gagccggccg agggcaagaa gaagtcccag atcgaggagt acctcgacta
     7501 ctaccgctcg ccgggagtcc agcacatcgc gatgcagacc gacgacatcg tgcggaccgt
     7561 gaccgccctc cgggagcgcg gcgtgcgctt catccgggtg cccgagacct actaccagga
     7621 cctcgccgag cgcgtcggtg agatcgagga ggactactcg gacctccaga agctggcgat
     7681 cctcgccgac cgcgacgagg agggctacct gctgcagatc ttcacgaaga tccagcagga
     7741 ccgtcccacg gtcttctacg agttcatcga gcgccacggc tcgcgcggct tcgggctcgg
     7801 gaacttcaag gagctgttca tcgcgatcga gcgggagcag gccgagcgcg ggaacctcta
     7861 gggccgggga ccgagcgtcc cccgttcgcc gggccgaacc gccctgtcac gatccctccg
     7921 ggcgcggcag gcttggggag ggaggaggtg acgccgatgc gcatcctcgt caggcacgtg
     7981 atgactcggg acgtcgcctc ggtctcggct tccgcgggat gcggcgacat cgcccggctg
     8041 atggtcgatc gcgaggtgac cacgatcccc gtggtggacc acgacggcag gctcgagggg
     8101 ctcgtgacgt cctcaggtct cctggagcac gtcggcggga agcgggagcc tctccacacg
     8161 ctggcggtgc tggcgactcc cggacggcac ccgcaccccc ttccgtgtga gctccgcgcg
     8221 gtcgacgtga tgtgccgtga gccggcggtg acgaggcggg aggccacggt gcccgaggcg
     8281 atcgaaaccc tggtgacgtc gcgggcgcgg gcgctcccgg tcgtggacga cgacgggagg
     8341 ctgctgggga tcatcgggag gagcgatctt ctgcgtggcc tggttcccat gatccgctcg
     8401 gtggacggtg tggtcggcct gcacccagcc cacggacggc ggcacgctcg agtggagcgc
     8461 ctgcgggcgt cgtgactagg ccaggccggt gatgccgcgc caggccaggc ggaccagcgc
     8521 gtccacggtg cggcgccggt cgtccttgga cgcgcccggg ttggcctggg cggccgtctc
     8581 cgccatcccg atcagcccca gcgccacgtc gcgggcgtcc tccatcggga ccttcccccg
     8641 ggattcccgg tggatgacct ccgtcacctc ggcgtggacg tcctcgcgga agcgctccac
     8701 catctcgcgg aagtcccgct ccgacccgac cgcctcggac atcagcagcc ggaacccctc
     8761 ggcgtgctcg tccacgaagt cgaagtaggc ctggaacgag gcccggatcc gctcgcggtt
     8821 gccccgggag gtctcgagcg cggtgtggac cgcctgcttc agcgaggcca ggtccgcctc
     8881 gagcagggcg aggtagaggt cgcgcttcga ggagaagtgg tcgtagagga tgggtttggt
     8941 gacccccgcc tccttggcca cggtgtccat cgtcaccgtg tggaagccgt tccggccgaa
     9001 gaccttccgg gcgacgtcga gcagctgctg tcttcgctcg ggagcccgca tcctccgccg
     9061 ctgcgccatt gacctccctc ctacctgctg gtaacctacc gcacagtagg ataggtaggc
     9121 tgaccgtccg ccattctgcc agagaggacg acccgatatg gagttccggc tcaacgagga
     9181 tcagctcacc gcccagaagt gggcccgcga gttcgcggag aaggagatcc gtccggtcgc
     9241 ccctgagtac gacgagtccg aggagttccc ctggcccgtc ctgaagaagg ccgcggagat
     9301 cgggctctac tccctggagt tctacgtgga gatggcgggc gccgatccca caggcctcat
     9361 catgccgatc gtcctggagg agtcgtgctg gggctgcgcg ggcatctcgc tcgggatctt
     9421 cgggacgggg ctcccgctgg ccacgctggc aggcaccggg acccccgacc agttcctgga
     9481 gtgggcgccg cggatgctgg ggacgtccga ggagccgaag atcggggcct tcgccgtcac
     9541 cgagtcgggc gccgggtccg acgtctccgc cctccgcacc accgcgaagc gcgacggcga
     9601 cgagtgggtg ctcaacggtc agaagatctt catcacgaac ggcgggatcg ccgacgtcca
     9661 catcgtggtg gccaccgtcg atccggagct ggggcatcgg ggccaggccg ccttcatcgt
     9721 cgggcccgac accccgggca tctcccaggg gaagaaggag cggaagctgg ggatccgggc
     9781 ctcgcacacg gccgaggtcc tcctcgacga ctgccgcgtc ccgctggaga acgtgggggg
     9841 cgggatggag aggctcgagg cgaagctggc gcgggcgcgg gagaatggca gcgagccgtc
     9901 ccggtcctcg caggcgctga agaccttcga gctcacgcgg ccgctggtcg cggcccaggc
     9961 cctggggatc gcacgggccg cgctcgagtt ctccgtggac tacgcgaagg agcggacgac
    10021 gttcgggcgc ccgatcatcg agcaccaggg gatcgggttc aagctggccg acatggccat
    10081 ggaggtcgac gcggctcgcc tcctgacgtg gagggccgcc tggatggtga agaacgatca
    10141 gctcgagcgg gccgagggct cgatgtccaa gctgaaggcc tccgaggtcg cggtctgggt
    10201 caccgagcag gcgatccagg tcctcggcgg ctacggctac atcaaggact tccccgtcga
    10261 gaagtggtac cgggacgcca agatctacac cctgttcgag gggaccagcg agatc
//
LOCUS       DASUTW010000168        20148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_440268, whole genome shotgun sequence.
ACCESSION   DASUTW010000168 DASUTW010000000
VERSION     DASUTW010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 20148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20148
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440268"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VF058_05770"
     CDS             complement(<1..619)
                     /locus_tag="VF058_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HEX6207853.1"
                     /translation="MRTEDQAAATGAEPAAERDRIRRGIRYCLLVFLGVRVTLTIVAL
                     VGTALLPSQEPVGPQGWEGSPLVGGWHNLVTSFERFDALWFLRVADQGYFDGDGSAVF
                     FPGYPLLIRGVSFLLGDRPLAAGLLVSNLAFAGALVVLYFLTSAEWGQRVARRSVLYL
                     AIFPTAFFFLAPYSESPFLLFALLAFWGARRGRWAVAGLAGAAAAA"
     gene            complement(616..1791)
                     /locus_tag="VF058_05775"
     CDS             complement(616..1791)
                     /locus_tag="VF058_05775"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX6207854.1"
                     /translation="MEAGRDRATLSIVFGVTLLTLTLGFLQKAPCLGPWDGRQYTLLC
                     YSDIVPLFHTERLDEGAIPYLEADNEYPVLTGMAMALAGIPADSPSSFFVWNALMLAG
                     LGLLAAAALARIAGRRALFFAAAPTLLIYAFVNWDLVAVAAATLGTWAFLRRRDGLAG
                     LALGVGAAAKLYPALLVIPFALQRLGERRTDRAGALAGAAAGAWVAINLPFAILAPER
                     WSIFFRFSAERPADWDSIWHVTQRHLGWPGSTEAVNLLSGLAFATLAAGLWLAASRTR
                     PGFPAWSFGLPLLVAFLLTGKVYSPQFSLWLLPWFGLALPHLPTFLAFSAAEVAVFVT
                     RFRWFAEMAGQDGGVPFWAFEAAILVRAAVLVWALVLWVRRPTPQPEPERRLQEAAT"
     gene            1838..3727
                     /locus_tag="VF058_05780"
     CDS             1838..3727
                     /locus_tag="VF058_05780"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEX6207855.1"
                     /translation="MSTRGRLVLILVALVPIVLISGAVAGLVGPLTKSYHFPRVLIEA
                     TIEPDGTLALRERRTYDFRGSFSSAFFTIDPTHAPTVNIRDFTVREGGRVIEHQEGAS
                     DRGGFQATWFYSAQDERRTFTISYRVRCAVDVYEDTAHLNWQFIGSGWDRLTDLARVT
                     VRVPDPARHPVARATTCPMPEGTAQLPPARSRPLREGEVRAWGHGSLAGTVRFEDPGT
                     VVLEVRDLRPATFVEGSIVLPLDSVPTAAASPGGPGLERILAQERRDADEANALRARH
                     RILDSLTTVLFFLVPALMLFVVVLAYRRDRVPGIPPTLQEPPEDVHPVKLALIWNAFH
                     KRLGARDAYRAQFLHLVHEGAIRLDAEGMVTDPKEIHIRRGRMPEEAMDRAFVRFLFA
                     EGEEPRLSRLRARGKRKELLADWWEVVGRVTKPSVTRISKGRSRAESWALAALAIGAG
                     VFGFWAWTGFSDELQPHGIVGTRAAWLIPVAVLSWLILSPFLRPYPSQTMRDRIARWR
                     AFRRFLRTFSTLEDAPTLAVIVWERYLVYAVALGVADRVETQVRGLIPEDRIAELAPE
                     SVPRSWHHWSDRVTHQSAYVATGAAATVGWSSGWGGSSSGGGGGGGFSGGGGGGGGGT
                     GGGAS"
     gene            3696..5552
                     /locus_tag="VF058_05785"
     CDS             3696..5552
                     /locus_tag="VF058_05785"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEX6207856.1"
                     /translation="MVAAPAAARRSLAAGALLLLALALGQPAAEAKSYRFPEVRIEAT
                     VLHDGTIELRERRTFEFDGSFSTAFFTVEPAGTPYARAEGFRVQEDGAEIPSTVSTGA
                     GGSLRAEWSFRAEDERRTFDISYRIRCAVLVYEDAAHLLWQFVGTGWEVPTDHVVVRV
                     RLPGRATAAPERPVGCPEAVPEAPATALLERGDVRAWGHGPLGGEVRLPDPQTVELEI
                     RDLEPFTFVEGSILMPAEVVPVAPSYPGGPGRERILAEEGRLAREANELRERRSRDRS
                     ISFGMLAGVPLAFLALVLISKARDRVPGVPEHLEEPPEDLHPVELATLWDAAAGRYVS
                     RNTYRTQLLHLARAGAVELEAVGRVTDPDDIVVRLKERPSSGIDEKFTSFLFPDSERT
                     VKLGDLEARGKRRKRIAAWKDAVTGKTSAMVDRVRKEGPRWESILMLVVVGVGIWWWT
                     RADLAGWAGLAVAGLAVLGWLVALRFMTPRLPPDLRERVARWAAFRRFLREFSSLPEA
                     PALAVVIWERYLVYASALGVADEVERQVADLVPPEELPSPWRGAPRGLSGLDLVHSMR
                     VATPLLAPSPPSPSGGSSFSSGVGSFSSSGGFGGGFSGGGGGGGGGTGGGAR"
     gene            5573..6169
                     /locus_tag="VF058_05790"
     CDS             5573..6169
                     /locus_tag="VF058_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207857.1"
                     /translation="MRRAWVLAISMALLTAACAEQPGDAGSTPTPTETPSPTVSPTPT
                     ATSDDLEDASCDDRQGGDPNNFPDFVEVSIESEGDTEKVEFRFEPEGDPSDAPQYFVG
                     FTDQLITDGEGAPVDVAGEAFVSISFMARGVDLSGEDFEEIYTGPTEFTPDLSTVLEV
                     EQTGDFEGMVSWGIGLSTRACFVVDARPDRIILEFPAS"
     gene            6241..7029
                     /gene="panB"
                     /locus_tag="VF058_05795"
     CDS             6241..7029
                     /gene="panB"
                     /locus_tag="VF058_05795"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX6207858.1"
                     /translation="MTTINDLRSFKAEGRRFAMLTAYDYPTARILDEAGIPVLLVGDS
                     LANVVLGHPTTLPVTMEEMLHHTRAVARGATSALVVGDLPFLSYQASQEDAIRNAGRF
                     LKEAGAHAVKLEGAHPELIERMVSAGIPVMGHLGLTPQSVHAMGGYRVQARTEEAAER
                     LLADAVQIEKAGVFSVVLEGVPSSVAKRVTESVAVPTIGIGAGPHCDGQVLVLHDLLG
                     LTFGKPAKFVKRYADVGEEISRAASAFKEEVEGGAYPAPEHSYD"
     gene            7057..7965
                     /locus_tag="VF058_05800"
     CDS             7057..7965
                     /locus_tag="VF058_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX6207859.1"
                     /translation="MASRILVTGASGTIGSRLVERLRAADVPVRLAGRSPEALRRRWP
                     PLDAVRLDALDPATIPAAVREVDVLYYLIHSMGEGEAGFAERDRRAAENVARAAAEAG
                     VRRIVYLGGLGDPGDSLSDHLASRHETGLALAAHGPPVVELRAGIILGADSASFRMLW
                     DLVDRLPVMVAPRWVDTRAQPIAIEDVITYLDAARTVETAQRHQIVEIGGADVISYKE
                     MMQRLARIRGKRRRIVTVPVLTPRLSSLWCAFVTSVPLSVARPLIDGVRNEVVVRDDT
                     ARLLFPDIRPVGFEAAARAAMEEMHR"
     gene            7962..8705
                     /gene="heR"
                     /locus_tag="VF058_05805"
     CDS             7962..8705
                     /gene="heR"
                     /locus_tag="VF058_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HEX6207860.1"
                     /translation="MRALRWWNAFLAVLHLVQGALMWAISNDFSLPVTAAFLTGGPEG
                     IVSEPETLFTVRIGPLVASFLFLSGLAHLSLATFARAWYERMLEREIQPARWIEYSAS
                     SSVMIVVIAMLTGVYDVAALLALFAVNASMILFGWVTEMRNEGRERIDWMPFWFGVFA
                     GAIPWIAIGIYLLGAGSDGAGPPGFVYGIYVSLFVFFNVFAINMVLQYRRVGRWRDYL
                     YGERAYMLLSLFAKSALAWQVFVGTLRPS"
     gene            complement(8706..9281)
                     /locus_tag="VF058_05810"
     CDS             complement(8706..9281)
                     /locus_tag="VF058_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019546770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX6207861.1"
                     /translation="MSSRRSIRHFAPDPVNPELIRLAIAAAASAPSGANVQPWRFVVV
                     TDPELKRRIRDGAEKEERDFYEARSPAEWLDALAPLGTDWRKPHLEDAPCLIVVFEVH
                     KGEGEPKPYYAKESVGIAVGFLLASLHHAGLATLTHTPSPMGFLGEILGRPPNERPFA
                     VIPVGRAAQEAEVPDITRKPLDEVMIWLEPR"
     gene            9538..9825
                     /gene="rpsF"
                     /locus_tag="VF058_05815"
     CDS             9538..9825
                     /gene="rpsF"
                     /locus_tag="VF058_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX6207862.1"
                     /translation="MRTYEVMIIVDESAEDKLEAVVERVTQVVTNGGGEVTKVDRWGK
                     RKFAYEIDHKTDGFYLLLEFTADTELLDELERVLLLADEVVRHKVVKREAA"
     gene            9897..10346
                     /locus_tag="VF058_05820"
     CDS             9897..10346
                     /locus_tag="VF058_05820"
                     /inference="COORDINATES: protein motif:HMM:NF005851.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX6207863.1"
                     /translation="MATENQITIVGNLTDDPELRYTPNGAAVANFRVAVSRRYRDPQT
                     NEWKDADTSFFTVNVWRSMAENAAESLTRGSRVVVVGRLKQRSWETAEGDKRTVIEIE
                     ADEVAPSLKWATAKIEKQSRGGGGGGDWNENVSVGAGGPAGETPVEG"
     gene            10363..10635
                     /gene="rpsR"
                     /locus_tag="VF058_05825"
     CDS             10363..10635
                     /gene="rpsR"
                     /locus_tag="VF058_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007319000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX6207864.1"
                     /translation="MARKQQQRQQKRRGREDRDDRKQQRKKYCYFCKEKTPIDYKDTA
                     LLRRFVSDRGKIRARRVTGNCAQHQRDVAVAVKNAREMALMPFSSR"
     gene            10632..11078
                     /gene="rplI"
                     /locus_tag="VF058_05830"
     CDS             10632..11078
                     /gene="rplI"
                     /locus_tag="VF058_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX6207865.1"
                     /translation="MKIILQKEVEKLGQPGDIVEVKDGYARNYLIPRGFATQATKGAV
                     LHAERVRREHETREAKALEQAAALAKRLEKTPVQVQAQAGEDGRLFGSVTTQQIAEAV
                     QRSIGEGVDRHEIRMDEPIRSTGTHEVLVHLHPEVNATITVQVVAQ"
     gene            11290..12639
                     /gene="dnaB"
                     /locus_tag="VF058_05835"
     CDS             11290..12639
                     /gene="dnaB"
                     /locus_tag="VF058_05835"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017604054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX6207866.1"
                     /translation="MARVAEEVPRPGGRVPPHNIEAEESVLGSMMLLEDAIAQVSEVL
                     QPDDFYRSAHRIIYETLIALFARGEPVDAITTVEELKRRSKLDAAGGALYIHHLVESV
                     PTPASAAHYARIVTELAMLRRLIEASARITQLAYDVPEDPVKLADQAESLIYSVARHQ
                     EKEEIVPLSTLVGESLETLERMHDREGGLAGLPTGFTDLDSLLQGLQPGNLIIIAARP
                     GVGKSSLVTNIARNVAVDAATPVAMFSLEMSRIEIAMRLLCAEARVGQDRVRSMRVAA
                     DEWPRIIEGAEVLDKAPFYIVDSGAMTIVDIRAKARRLKSSQGLGLLIVDYLQLMSSH
                     QRVESRQQEIAEISRSLKLLAKELEIPVIAVSQLNRNPESRTDKRPMLADLRESGSLE
                     QDADVVMFIHREELWSDDPNVKGLAEVIVAKHRNGPTDKISLTFLSHLTQFRNFARG"
     gene            12658..13353
                     /gene="nth"
                     /locus_tag="VF058_05840"
     CDS             12658..13353
                     /gene="nth"
                     /locus_tag="VF058_05840"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX6207867.1"
                     /translation="MSTGAEATTDAGRLREIHRRLRRVHGPLDPPRKRDPLEELVLTI
                     LSQNTSDVNRDRAFEAMRRRFPTWEEVADAPIAKLRDAIRPGGLANTKAPRIQQVLRE
                     IRAREGRLDLGWMRRAGDEEVEEYLVSLPGVGPKTAACVLAFSLGRPALPVDTHVYRV
                     AGRLGLLPAGSTPDRAHRALKSLVAPAMRIPLHVGLIRHGRTICKAGVPRCEVCPLSD
                     ICPSASRFLDDGA"
     gene            13612..14676
                     /locus_tag="VF058_05845"
     CDS             13612..14676
                     /locus_tag="VF058_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017249503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207868.1"
                     /translation="MALSFLGTLVALMMTASSRLPEPGNPLTLPNEREVQAIRVPTPD
                     GGAGIAFVPVPVPGSQAGSPSSAAAPLLGGIGTPLGGGSEGTVPPGPPNEGPGEEPGP
                     GEEPGDGEEPGDGEQPGEDPGDDDGVENPDDGGPRRPQEPEEPDGDDGDGDGRGSCPD
                     GPPHGKGPGQGKGSEDEDGGPPPHGKGPGEGKCKDSPPHGKGKGPGNGKGKDSGKGSG
                     NDEGGGSGGGKGKGSGNGGGNGGGKGKGSGNGGGNGGGNGGGKGKGSGNGGGDGGGKG
                     KGKGSGNDEGGGNGGGRGKGSGNGNGNGNGNGNGNGNGNGNGNGNGNGNGNGNGNGNG
                     NGNGNGNGNGNGNTRAKAKR"
     gene            complement(14685..14755)
                     /locus_tag="VF058_05850"
     tRNA            complement(14685..14755)
                     /locus_tag="VF058_05850"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14721..14723),aa:Gly,seq:ccc)
     gene            complement(14793..16823)
                     /locus_tag="VF058_05855"
     CDS             complement(14793..16823)
                     /locus_tag="VF058_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX6207869.1"
                     /translation="MLGPIILLGLLALAGGIFMQRGYFLFRLVTLGRPVKRTEDLPRR
                     LEKEAVVVFGQRKLLQRIGPGLMHAFIFWGFLVLLTTIIEVGGEIFDESFAIPWVGRT
                     AWLGFVQDLFAGLVLVGIGMALYFRKVRRDRRFVGSHLEEADFILVLIFGVILTLFFL
                     NATKIAMGANESPAAWTPISNFIAPLFETMTRDAQNLFRQIFLWSHIAVILGFLAYIP
                     FSKHLHILTSSINVFFSKTKPMGKLETIDIDLENLEESRLGAATVEDLTWKQMLDTYT
                     CTECGRCQNVCPAWNTGKPLNPKLLIMNLRDHLFDHGGDILQARKRGEQYEQVPLVPD
                     VVEEEVVWDCTTCGACMQECPVNIEHVDHILDMRRNLVMAESSFPTEAGLLLRNLESS
                     KNPWGQSQSSRADWAEDLGVQIVEDGDAPEYLYWVGCASSFDDRSRKIARAFTRLLQK
                     AGVRFGILGPRELCNGDPARRMGNEFLFQELARENVETLNSIGTKKIVAACPHCFNTL
                     RNEYPDFGGDYEVLHHTELLARLVDQGRLTPTEEVRALLTYHDPCYLGRHNGVYDEPR
                     SALESVPGLRTVEMERHGARGFCCGAGGARMWMEEPIGKRVNMERTDEAAATGADILG
                     VACPYCMVMLDDGAKAKGSDMQVLDVAQVLENSIFGEQGPSEAMAERSGSSA"
     gene            16945..17601
                     /locus_tag="VF058_05860"
     CDS             16945..17601
                     /locus_tag="VF058_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207870.1"
                     /translation="MSRLRSALGIFVSVAVLVAAGVAGARGTFFTSDPVDEGMEEVVG
                     DPDPCAPEEGEGEEGEPKGEGEEGEPGECEPEEEPEGEELEGDDEGEQGEAEVGQGER
                     DAACDAAAGIEETPLPEGEKLTGLDNALSRVLENCKKNPQAPGLLNALERLSENRARK
                     AARDEAKAERRAEREARKAERKAAKGQSGGPPQGGGPPPHAGPPGGGGPPGHAKGGKG
                     "
     gene            17831..19669
                     /gene="dnaK"
                     /locus_tag="VF058_05865"
     CDS             17831..19669
                     /gene="dnaK"
                     /locus_tag="VF058_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX6207871.1"
                     /translation="MARAVGIDLGTTNSVVSVLEGGEPTVIPNAEGSRTTPSVVGFSK
                     SGEILVGEVAKRQAITNPDRTVRSVKRHMGSKDWDVEIDDKRWTPQEISAQILTKLKR
                     DAEAYLGDTVVQAVITVPAYFDDAQRQATKEAGQIAGLEVLRIINEPTAASLAYGLDK
                     EQHDQTILVFDLGGGTFDVSLLEIGEGVFEVKSTSGNTQLGGDDWDQRIIDWMVTEFK
                     NAHGVDLSRDRMALQRLQEAAEKAKIELSQVTETQINLPFVTATNEGPLHLDMRLTRS
                     EFERMTEDLVQAAREPFEQAVKDWGKEVSEIDHIILVGGSTRMPMIQELVRGLTGGKE
                     PHKGVNPDEVVAVGAGIQAGVLKGEVKDVLLLDVTPLSLGIETKGGIFTKLIERNTTI
                     PTKKSEIFTTADDSQTQVEVHVLQGEGEMVDSRANRSLGRFHLTGIPPAPRGMPQIEV
                     TFDIDANGIVNVSAKDTATGKEQAMTITGGTALDRDELDRMMKDAEQYAEEDHRRREA
                     AEARNQADNLAYQAEKTVKELGDKVPEDDRQVVESAVADVRKALEGDDLDEIKAKTEA
                     LTEAFQKVGQAVYQQQQAAEGGAEQEAAEGEEDVVEGEVVDEGGSS"
     gene            19666..>20148
                     /locus_tag="VF058_05870"
     CDS             19666..>20148
                     /locus_tag="VF058_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX6207872.1"
                     /translation="MTDEQRPDDVVEEVVEGEVEEAPDTSELDEAREQAAEYLDHLQR
                     LKAEFENYRKRVLKEQTRAIEIASEDLVLKLLSVLDDFELALVAAEQKPDFEKFLQGV
                     EMVYAKLVDVLRSEGLEKIEAKGKPFDPELHEALFQGGEEDGDPYVADVLRTGYTLKG
                     K"
BASE COUNT         3257 a         6761 c         7317 g         2813 t
ORIGIN      
        1 tcgcggccgc ggcggcgcct gcgaggccgg cgaccgccca gcggccgcga cgcgcccccc
       61 agaacgccag cagggcgaag agcaggaacg gcgactccga gtacggcgcc aggaagaaga
      121 acgcggtggg gaagatggcc aggtagagca ccgaccgccg ggccacgcgc tggccccact
      181 cggcgctcgt caggaagtag aggacgacga gcgcccccgc gaaggccagg ttcgagacga
      241 gcaggccggc cgccagcggg cggtccccca gaaggaacga gacgccgcgg atcagcagcg
      301 ggtagcccgg gaagaacacc gcgctcccgt ccccgtcgaa gtagccctgg tctgcgaccc
      361 gcaggaacca cagcgcgtcg aaccgctcaa aggacgtgac gaggttgtgc cagccgccga
      421 cgagcgggga gccctcccac ccctgaggcc cgacgggctc ctgcgagggg aggagcgccg
      481 tcccaacgag cgccacgatc gtgagcgtca cccggacgcc caggaacacg agcaggcagt
      541 agcggatccc gcggcggata cggtcgcgct cggccgcggg ctcggccccg gtggcggcgg
      601 cctggtcctc ggtcctcacg tcgcggcctc ctggagacgg cgctcgggct cgggctgtgg
      661 cgtagggcgg cgcacccaca ggacgagggc ccagaccagc acggccgccc ggaccaggat
      721 cgccgcctcg aacgcccaga agggtacccc gccgtcctgc cccgccatct cggcgaacca
      781 gcggaagcgc gtgacgaaga ccgcgacctc ggccgcggag aacgccagga aggtcgggag
      841 atggggcagg gccagcccga accaggggag cagccacagg ctgaactggg gcgagtagac
      901 cttccccgtg agcaggaagg cgaccagcag cggaagcccg aagctccagg cggggaagcc
      961 gggccgggtc ctggacgccg cgagccagag cccggccgcg agtgtcgcga aggccagccc
     1021 ggacaggagg ttcacggctt cggtggaccc gggccagccc aggtgccgct gcgtcacgtg
     1081 ccagatcgag tcccagtcgg cgggccgctc ggcgctgaac cggaagaaga tcgaccatcg
     1141 ctccggggcg aggatcgcga aggggaggtt gatcgcgacc cacgcgccgg cggccgcccc
     1201 cgccagcgcc ccggcccggt ccgtccgccg ctccccgagc cgctggaggg cgaacgggat
     1261 cacgaggagc gcggggtaga gcttggcggc cgcccccacg cccagggcca ggcccgccag
     1321 gccgtcgcgg cggcggagga aggcccacgt gcccagggtc gccgcggcga cggccaccag
     1381 atcccagttg acgaaggcgt agatcagcag cgtgggggcg gccgcgaaga agagcgccct
     1441 tcgcccggcg atcctcgcca gcgccgcggc cgccagcagc cccagacccg ccagcatcag
     1501 cgcgttccac acgaagaagg acgacgggga gtccgcgggg atccccgcca gcgccatcgc
     1561 catccccgtg aggaccgggt actcgttgtc ggcctcgagg tacgggatcg cgccctcgtc
     1621 cagccgctcg gtgtggaaga gggggacgat gtccgagtag cagagcaggg tgtactgcct
     1681 cccgtcccac ggtcccaggc acggggcctt ctgcaggaac ccgagggtca gggtgaggag
     1741 cgtgaccccg aagacgatcg agagggtcgc gcggtcgcgg cctgcctcca ccgtccgatt
     1801 atccgtgacc cgagcgacct gtcggtacac ttcggccatg tcgacgaggg ggaggctcgt
     1861 gctgatcctc gtcgccctcg tgccgatcgt gctcatcagt ggtgcggtcg cggggctcgt
     1921 cgggccgttg accaagagct accacttccc ccgggtcctg atcgaggcca cgatcgagcc
     1981 ggacggcacg ctcgccctcc gcgagcgccg cacctacgac ttccggggct cgttctcctc
     2041 ggccttcttc acgatcgacc ccacccacgc gcccacggtg aacatccggg acttcaccgt
     2101 gcgcgagggg ggccgcgtga tcgagcacca ggagggcgcc tcggaccggg gcggcttcca
     2161 ggcgacgtgg ttctattcgg cccaggacga gcgccggacc ttcacgatct cctaccgggt
     2221 ccgctgcgcc gtggacgtct acgaggacac cgcgcacctg aactggcagt tcatcgggtc
     2281 gggctgggac cgcctcaccg acctcgccag ggtcacggtc cgcgtgcccg atcccgcccg
     2341 ccatcccgtc gcccgcgcca ccacgtgccc catgcccgag gggaccgccc agctgccccc
     2401 cgctcgcagc cggcctctcc gtgaggggga ggtgcgcgcc tgggggcacg ggtcgctggc
     2461 gggcaccgtg cggttcgagg atccgggaac cgtcgtcctc gaggtccgcg acctgaggcc
     2521 cgccaccttc gtggagggct cgatcgttct cccgctcgat tcggtcccca cggctgccgc
     2581 ctccccgggc gggccggggc tcgagcgcat cctggcgcag gagcggcgag acgcggatga
     2641 ggccaacgct ctccgcgccc gtcaccggat cctcgactcg ctcaccacgg tcctcttctt
     2701 cctcgtcccc gcgctgatgc tcttcgtcgt ggtcctcgcc tatcggcgcg accgcgtccc
     2761 gggcatccca ccgacgctcc aggagccccc cgaggacgtc caccccgtca agctcgccct
     2821 catctggaac gcctttcaca agcgcctcgg ggcccgcgac gcctaccggg cccagttcct
     2881 ccacctggtg catgaggggg cgatccgcct cgacgccgag gggatggtca ccgaccccaa
     2941 ggagatccac atccgccggg gccggatgcc tgaggaggcg atggaccgag ccttcgtgcg
     3001 cttcctcttc gcggagggcg aggagccgag gctctcccgg ctgcgggcga gggggaagcg
     3061 gaaggagctc ctcgccgact ggtgggaggt cgtgggccgg gtgacgaagc cgagcgtgac
     3121 gaggatctcg aaggggagga gccgggccga gtcctgggcg ttggccgccc tggcgatcgg
     3181 ggccggggtc ttcggtttct gggcctggac cggattctcc gacgagctcc agccgcacgg
     3241 gatcgtcggg acgcgagccg cgtggctgat cccggtcgcg gtcctctcct ggctgatcct
     3301 cagccccttc ctccgcccgt acccctcgca gacgatgcgc gatcggatcg cccggtggcg
     3361 ggcgttccgc aggttcctgc ggacgttctc cacgctcgag gacgccccga ccctcgcggt
     3421 gatcgtgtgg gagcgctacc tcgtgtacgc ggtggccctc ggggtggcgg accgcgtgga
     3481 gacgcaggtc cggggcctga tccccgagga ccggattgcg gagctggcgc cggagtccgt
     3541 ccctcggtcc tggcaccact ggagcgaccg ggtgacccat cagagcgcgt acgtggcgac
     3601 gggggcggcg gccacggtgg gctggtcgag cgggtggggc ggctcttcct cgggcggtgg
     3661 cggtggcggt gggttctccg ggggaggggg tggcggtggt ggcggcaccg gcggcggcgc
     3721 gtcgtagcct cgcggccggg gcgctgctgc tcctggcgct ggccctcggc cagcctgcgg
     3781 ccgaggcgaa gtcctaccgc ttccccgagg tccggatcga ggccacggtc ctccacgacg
     3841 gcacgatcga gcttcgggag cgccgcacct tcgagttcga cgggtccttc tccaccgcct
     3901 tcttcaccgt cgagcccgcg gggacgccct atgccagggc cgagggcttc cgggtccagg
     3961 aggacggcgc ggagatcccc tccacggtga gcacgggcgc cggcggctcc ctccgcgcgg
     4021 agtggtcctt ccgcgccgag gacgagcgcc gcaccttcga catctcctac cggatccggt
     4081 gtgccgtcct cgtctacgag gacgccgcgc acctcctctg gcagttcgtc ggcacgggat
     4141 gggaggtccc gaccgaccac gtcgtggtcc gggtgcgcct gcccggtcgg gccaccgcag
     4201 cgcccgagcg tcccgtcggc tgccccgagg ccgttcccga ggcgccggcc accgccctcc
     4261 ttgagcgcgg cgacgtccgg gcctggggcc acggtccgct cgggggcgag gtccgccttc
     4321 cagatcccca gacggtggag ctcgagatcc gggacctcga gcctttcacc ttcgtggagg
     4381 ggtcgatcct gatgccggcc gaggtcgtgc cggtcgcccc ctcgtacccg ggcggcccgg
     4441 gacgggagcg catcctcgcg gaggagggac gcctggcccg ggaggccaac gagctgcggg
     4501 agcgacgctc gcgggaccga tcgatctcct tcgggatgct ggccggggtc ccgctcgcgt
     4561 tcctcgccct cgtcctgatc tcgaaggcgc gggaccgggt gccgggggtg cccgagcacc
     4621 tcgaggagcc gcctgaggac ctccacccgg tcgagctggc cacgctctgg gacgctgccg
     4681 ccggccgcta cgtctcgcgc aacacctatc gaacccagct cctccacttg gcccgggccg
     4741 gggccgtcga gctcgaggcc gtgggacgcg tcaccgatcc cgacgacatc gtcgtgcggc
     4801 tcaaggagcg gccgtcctcg ggcatcgacg agaagttcac gagcttcctt ttcccggact
     4861 cggagcggac cgtgaagctc ggcgacctcg aggctcgggg gaagcgccgg aagcggatcg
     4921 ccgcctggaa ggacgccgtg accgggaaga cgagcgcgat ggtggaccgg gtccgcaagg
     4981 aagggccccg ttgggagtcg atcctgatgc tcgtggtcgt cggcgtcggg atctggtggt
     5041 ggacgagggc ggacctggcc ggatgggcgg gtctcgccgt ggcagggctc gcggtactgg
     5101 gatggctcgt ggcgctccgg ttcatgacgc cgcggctccc cccggatctc cgggagaggg
     5161 ttgcccgctg ggcggcgttc cggcgcttcc tccgggagtt ctcctctctc cccgaagccc
     5221 ccgcgctggc cgtggtgatc tgggagcgat acctcgtcta cgccagcgcc ctcggggtgg
     5281 ccgacgaggt ggagcgacag gtcgccgacc tcgtcccgcc agaggagctt ccctcgccgt
     5341 ggcggggagc gcctcgaggc ctgtccgggc tggacctcgt gcattcgatg cgcgtggcca
     5401 cacccctcct ggccccgtcg cctccgtccc cgagcggcgg ctcgtcattc tccagcgggg
     5461 ttggatcctt cagctcgtcg ggggggttcg gcgggggctt ctccggagga ggaggagggg
     5521 gcggcggcgg gaccggcggc ggagcccgct gaacgtgcct acactgatgg gcgtgcgacg
     5581 cgcgtgggtc ctggcgatct cgatggcgtt gctgacggcg gcctgcgccg aacagccggg
     5641 ggacgccgga tccactccca cgcccaccga gacgccgtcc cccacggtct ctcccacgcc
     5701 caccgcgacc tcggacgacc tcgaggacgc ctcgtgcgac gaccggcagg gcggggaccc
     5761 gaacaacttc cccgacttcg tcgaggtgag catcgagtcc gagggcgata ccgagaaggt
     5821 ggagttccgc ttcgagcccg agggcgaccc gagcgacgcc ccacagtact tcgtggggtt
     5881 caccgatcag ctgatcaccg acggtgaggg tgcccctgtc gacgtcgcgg gcgaggcgtt
     5941 cgtgtcgatc tcgttcatgg cgcgaggtgt ggacctctcg ggcgaggact tcgaggagat
     6001 ctacaccggg cccaccgagt tcactccgga tctctccacg gtcctcgagg tggagcagac
     6061 cggggacttc gagggcatgg tctcgtgggg catcgggctc tcgacgcggg cctgcttcgt
     6121 ggtggacgcc cgccccgatc gcatcatcct cgagttcccc gcgtcctgac gcttccccgt
     6181 gccggccccg ccggggtacc cttgtcgtgc cgtgcactct cacggaagga ggcgcgagcc
     6241 gtgaccacga tcaacgacct gcggtcgttc aaagccgagg gcaggcgctt cgccatgctc
     6301 acggcctacg actacccgac cgcgcgcatc ctcgacgagg cgggcatccc cgtgctcctc
     6361 gtgggggact cgctggccaa cgtggtgctg gggcatccca ccacgctgcc cgtgacgatg
     6421 gaggagatgc tccaccacac gcgggcggtg gcccgcggcg cgacgagcgc gctcgtggtg
     6481 ggcgatctcc cgttcctctc gtatcaggcc tcgcaggagg atgcgatccg caacgcggga
     6541 cggttcctca aggaggccgg cgcgcacgcg gtgaagctcg agggcgcgca ccccgagctg
     6601 atcgagcgga tggtgtcggc aggcatcccg gtgatgggcc acctgggcct gaccccgcag
     6661 tccgtccacg cgatgggcgg gtaccgggtg caggcccgca ccgaggaggc cgccgagcgc
     6721 ttgctcgccg acgcggttca gatcgagaag gcgggggtgt tctcggtggt gctcgagggc
     6781 gtcccctcgt cggtggcgaa gcgggtgacc gagtcggtcg ccgttcccac gatcgggatc
     6841 ggtgccggtc cgcactgcga cggccaggtc ctcgttctgc acgacctcct gggactgacc
     6901 ttcgggaagc ccgcgaagtt cgtgaagcga tacgcggacg tgggggagga gatctcccgg
     6961 gccgcctcgg ccttcaagga ggaggtcgag gggggagcct acccggcccc cgagcactcc
     7021 tacgactgac ccgccgcgcc gtaggatgtc cccatcgtgg cgtctcgcat cctcgtcacg
     7081 ggggcgagcg gcaccatcgg ctcgcggctg gtggagcgcc tccgcgccgc cgacgttccc
     7141 gtccgcctgg ccgggcggag cccggaggcc ctccgccggc gatggccgcc cctcgacgcg
     7201 gtccgcctcg acgccctcga tcccgcgacc atccccgccg cggtgaggga ggtagacgtc
     7261 ctctactacc tgatccactc gatgggggag ggggaagccg ggttcgccga gcgcgatcgg
     7321 cgggcggcgg agaacgtcgc acgggcggcg gcggaggcgg gtgtgcggag gatcgtctac
     7381 ctcggagggt tgggcgatcc cggcgacagc ctctccgatc acctcgccag ccgccatgag
     7441 accgggctcg cgctcgctgc ccacggcccg cccgtcgtcg agctccgcgc cgggatcatc
     7501 ctcggggcag acagcgcctc gttccggatg ctgtgggacc tggtggaccg gctgccggtc
     7561 atggtggccc ccagatgggt ggacacccgc gcgcagccca tcgccatcga ggacgtcatc
     7621 acgtacctcg acgcggcgag gaccgtggag acggcccagc gccaccagat cgtggagatc
     7681 ggcggcgccg acgtcatctc ctacaaggag atgatgcagc gactggcgcg gatccggggc
     7741 aagcggaggc ggatcgtcac cgtgcccgtc ctcacgccga ggctctcctc gctgtggtgc
     7801 gccttcgtga cctcggtgcc gctctcggtg gctcgtcctc tgatcgacgg cgtgcgcaac
     7861 gaggtcgtcg tccgcgacga caccgcacgt ctgctgttcc ccgacatccg acccgtcggc
     7921 ttcgaggccg cagcccgcgc ggcgatggag gagatgcatc gatgagagcg ctgcggtggt
     7981 ggaacgcctt tctggccgtc ctgcacctcg tccagggcgc tttgatgtgg gcgatctcga
     8041 atgacttctc cctcccggtg accgcggcct tcctgaccgg cggccccgag gggatcgtct
     8101 ccgagccgga gaccctgttc acggtgcgca tcgggccgct ggtggcaagc ttcctcttcc
     8161 tctcgggcct ggcccacctc tcgctggcaa cgttcgcccg cgcctggtac gagcggatgc
     8221 tggagcggga gatccagccg gcgcggtgga tcgagtactc cgcgtcctcc tcggtgatga
     8281 tcgtcgtgat cgccatgctg accggggtct acgacgtggc ggcgctgctc gcgctcttcg
     8341 ccgtgaacgc ctccatgatc ctcttcggct gggtgaccga gatgcggaac gagggccggg
     8401 agcggatcga ctggatgccg ttctggttcg gggtcttcgc cggcgcgatc ccctggatcg
     8461 cgatcgggat ctacctgctc ggcgcgggta gcgacggggc cggaccgcca ggcttcgtgt
     8521 acgggatcta cgtctcgctg ttcgtcttct tcaacgtgtt cgccatcaac atggtcctgc
     8581 agtaccgtcg cgtgggccgg tggcgcgact acctctacgg ggagcgggcc tacatgctgc
     8641 tgtcgctctt cgccaagtcg gcgctggcgt ggcaggtgtt cgtggggacc ctccggccga
     8701 gctgatcacc tcggctcgag ccagatcatc acctcgtcca ggggcttccg cgtgatgtcg
     8761 gggacctcgg cctcctgggc cgcccgcccc acggggatca ccgcgaacgg ccgctcgttg
     8821 ggcgggcggc ccaggatctc gccgaggaac cccatcgggc tcggggtgtg ggtcagcgtg
     8881 gcgagtccgg cgtggtggag ggaggcgagg aggaacccca cggcgatccc caccgattcc
     8941 ttcgcgtagt agggcttggg ctcgccctcc cctttgtgca cctcgaacac cacgatgagg
     9001 cacggagcgt cctcgaggtg gggcttgcgc cagtcggtcc ccagcggggc gagagcgtcg
     9061 agccattccg ccggtgatcg ggcctcgtag aagtcccgct cctccttctc ggccccgtcc
     9121 cggatcctcc gcttgagctc ggggtcggtg acgacgacga accgccacgg ctggacgttc
     9181 gccccggatg gggcggaggc tgcggcggcg atcgccagcc tgatcagctc gggattgacg
     9241 gggtcgggcg cgaagtggcg gatcgagcgc cgggaggaca tcgtctcgag gaatcctcgg
     9301 gccgcggcga gcgcatcctc gggggtcagc tcccggcggg gggccgggat ggttcggtgg
     9361 cgagggtcgc acgtcacgtc cggtttccta ccatgctttc caggaggtcg ggaagccctc
     9421 gtccatgtca gaccccttcg ctatggtgcc ggatgacatc gccgtggccg gtgctagact
     9481 gcgtgggacc ccactcctgc tcccccgggg ggccgcatga gtccagagga ggtggcagtg
     9541 cgaacgtacg aggtcatgat catcgtcgac gagtccgccg aggacaagct cgaggcggtc
     9601 gtggagcgcg tcacgcaggt cgtcacgaac ggcggcggcg aggtcacgaa ggtcgaccgc
     9661 tggggcaaac ggaagttcgc ctacgagatc gaccacaaga cggacgggtt ctacctcttg
     9721 ctcgagttca cggccgacac tgagctgctc gacgagctcg agcgggtcct gctgctggcc
     9781 gacgaggtcg tccgtcacaa ggtcgtgaag cgggaggctg cgtagacgaa gtcttcccct
     9841 cctcccggcg gaggccatcg gccgggaggg cgagcgaggc gaggtggtga tgccggatgg
     9901 ccacggagaa ccagatcacg attgtcggga acctgaccga cgaccccgag ctgcggtaca
     9961 cgcccaacgg cgcggccgtg gccaacttcc gcgtggcggt gagccgccgc taccgggacc
    10021 cgcagacgaa cgagtggaag gacgcggaca cgtcgttctt caccgtgaac gtctggcgct
    10081 cgatggccga gaacgccgcc gaatcgctga cgaggggctc gcgcgtggtg gtcgtgggtc
    10141 gcctgaagca gcgctcctgg gagaccgccg agggcgacaa gcgcacggtg atcgagatcg
    10201 aagccgacga ggtcgcgccc agcctgaagt gggccacggc gaagatcgag aagcagagcc
    10261 ggggcggcgg aggtggcggc gactggaacg agaacgtgag cgtcggggcc gggggaccgg
    10321 ccggggagac gccggtcgaa ggctgagaag gagggaacga agctggcacg gaagcagcag
    10381 cagagacagc agaagaggcg aggacgcgag gatcgggacg atcggaagca gcagcggaag
    10441 aagtactgct acttctgcaa ggagaagacc ccgatcgact acaaggacac ggctctcctg
    10501 cggaggtttg tctccgaccg ggggaagatc cgagctcgcc gggtgacggg caactgcgct
    10561 cagcaccagc gcgacgtggc cgtggccgtg aagaacgccc gcgagatggc cctgatgccg
    10621 ttctcgagcc gatgaagatc atccttcaga aggaggtcga gaagctcggt cagcccggcg
    10681 acatcgtcga ggtgaaggac ggctacgcgc ggaactacct gatccctcgc ggcttcgcga
    10741 cccaggccac gaagggggcg gtcctgcatg ccgagcgggt tcgcagggag cacgagaccc
    10801 gcgaggccaa ggccctagag caggcggccg cgctcgccaa gcgcttggag aagacgccgg
    10861 tccaggtgca ggcgcaggcg ggcgaggacg gacggctctt cggcagcgtg accacccagc
    10921 agatcgccga ggccgtccag cggtcgatcg gcgagggggt ggaccggcac gagatccgga
    10981 tggacgagcc gatccgttcc accggaacgc acgaggtcct ggtccatctc cacccggagg
    11041 tcaacgccac gatcacggtg caggtcgtcg cccagtagaa catctcgaac ccggggtcga
    11101 cgttcgtgtc agaccccggg attttcatgg aacacgtgtt cgggcatgtg gaaatccgga
    11161 gcgcaggtca cgcacggggg gtgcggacgt tccacaggct ctccacaacg cgtccaccgc
    11221 gtccggccgc ttatcccatg gataccttca gcggcgctcc gctagggtca ggtggcgaag
    11281 ggggcggaga tggcacgggt cgctgaggag gtcccacgtc cgggcgggcg ggtcccgccg
    11341 cacaacatcg aggccgagga gtcggtcctc ggatcgatga tgctcctgga ggacgccatc
    11401 gctcaggtga gcgaggtcct ccagcccgac gacttctacc gctcggcgca ccggatcatc
    11461 tacgagaccc tcatcgcgct cttcgcccgc ggggagcccg tggacgccat caccaccgtc
    11521 gaggagctga agcgccggag caagctcgac gcggcggggg gcgctctcta catccatcac
    11581 ctggtcgagt cggtgccgac ccccgccagc gccgcgcact acgcccggat cgtgaccgag
    11641 ctggcgatgc tcaggcgcct gatcgaggcc agcgccagga tcacccagct cgcctacgac
    11701 gtccccgagg accccgtgaa gctcgcggac caggccgaga gcctcatcta ctccgtggcc
    11761 cgccatcagg agaaggagga gatcgtcccg ctcagcacgc tcgtgggcga gtcgctggag
    11821 acgctggagc ggatgcacga ccgggagggc gggctggctg gcctccccac cgggttcacc
    11881 gacctcgact cgctgctcca ggggctccag ccgggcaacc tcatcatcat cgccgccagg
    11941 cccggggtcg ggaagtcctc cctcgtcacg aacatcgccc gcaacgtggc ggtggacgcg
    12001 gccacgccgg tcgccatgtt ctccctcgag atgtctcgga tcgagatcgc gatgcggctc
    12061 ctatgcgccg aggctcgcgt gggtcaggac cgcgtgcgct cgatgcgcgt agccgccgac
    12121 gagtggccca ggatcatcga gggcgcggag gttctcgaca aggcgccgtt ctacatcgtg
    12181 gactccggcg ccatgacgat cgtcgacatc cgggccaagg cccgccgcct caagtcgtcg
    12241 cagggcctgg gcctcctcat cgtcgactac ctccagctga tgtcgagcca ccagcgcgtg
    12301 gagagccgcc agcaggagat cgccgagatc tcacgctcgc tgaagctcct ggccaaggag
    12361 ctggagatcc cggtgatcgc cgtctcccag ctcaaccgca accccgagag ccgcaccgac
    12421 aagcggccga tgctcgccga cctccgcgag tccgggtcgc tggagcagga cgccgacgtc
    12481 gtgatgttca tccaccggga ggagctctgg agcgacgatc cgaacgtgaa ggggctggcc
    12541 gaggtgatcg tggccaagca ccggaacggc cccaccgaca agatctcgtt gacgttcctc
    12601 tctcacctga cgcagttccg gaacttcgct cgcgggtagc gcggtagggt tgcgggcgtg
    12661 agcaccggcg ccgaggccac gaccgacgcc gggcgcctcc gcgagatcca tcgccgcctg
    12721 cggagggtgc acggcccgct cgaccctccc cgcaagcgcg atccgctcga ggagctcgtg
    12781 ctcacgatcc tctcccagaa cacctccgac gtgaaccgag accgagcctt cgaggcgatg
    12841 cgccgccggt tcccgacgtg ggaggaggtc gccgacgcgc cgatcgcgaa gctgcgcgac
    12901 gcgatccgcc ccggcggcct ggccaacacc aaggccccga ggatccagca ggttcttcgt
    12961 gagatccggg ctcgcgaggg caggctggac ctcggctgga tgcgaagggc cggcgacgag
    13021 gaggtcgagg agtacctcgt gagcctcccc ggtgtgggcc cgaagacggc cgcgtgcgtc
    13081 ctcgccttct cactcggacg cccggcgctt ccggtcgata cccacgtgta ccgggtggcc
    13141 gggcgactcg gcctccttcc cgccgggtcg acccccgacc gagcccaccg ggcgctgaag
    13201 agtctcgtgg ccccggcgat gcgcatcccc ctgcatgtcg ggctgatccg ccacgggagg
    13261 acgatctgca aggcgggcgt cccgagatgc gaggtctgcc ccctatcgga catatgcccc
    13321 agcgcctcgc ggttcctcga cgacggcgcc tgatgtgggg gaactggcga ccacctggaa
    13381 gccgacatcg acgcggtcgc gctatgccgc ccctcaccgc gcccgggtcg atgctcgcct
    13441 tagaggaatc ttcacgagaa aggatggcgg aggtcagcga aagggtagac tgcgcgcagc
    13501 cgggttcaga ttctcggtcg aaggtccgat agggagacta ggttgcgacc tcgatccgcg
    13561 cgtgtggagg gcagcggggc ggcgcgccgg gcaggtgtga ccggcgccat cttggcgttg
    13621 tcattcctgg gcaccctggt ggccctcatg atgacggctt cttctcgcct gcccgagccc
    13681 gggaaccctc tcacgctccc caacgagcga gaagtccagg cgatcagggt ccccacgccc
    13741 gacggaggag ccggcatcgc cttcgtcccc gtccccgtcc ctggctctca ggcgggatcg
    13801 ccctcgtccg cggcggcccc gctcttgggg gggatcggca cgccgctggg gggaggctcc
    13861 gagggcacgg tgcctcccgg ccccccgaac gagggtccgg gcgaggagcc tggccccggt
    13921 gaggagcccg gggacggtga ggagcccggg gacggggaac agccgggtga ggatcccggt
    13981 gacgacgacg gtgtcgagaa ccccgacgac ggcggaccgc ggcggccgca ggagcccgag
    14041 gagccggacg gcgacgacgg cgatggcgac ggccggggca gctgcccgga cggtccacct
    14101 cacggcaagg gtcccggcca ggggaaggga tccgaggacg aggacggagg tcctcccccc
    14161 cacgggaagg gccccggtga ggggaagtgc aaggacagcc ctccccatgg gaagggcaag
    14221 ggacccggga acgggaaggg caaggactct ggtaaggggt ccgggaatga cgagggcggc
    14281 ggcagcggcg ggggcaaggg taagggttcc ggcaatggcg gcgggaacgg cggaggcaag
    14341 ggcaagggtt ccggcaacgg cggcggcaac ggcggcggga acggcggagg caagggcaag
    14401 gggtccggga acggcggcgg tgacggcgga ggcaagggca aggggaaggg atccgggaac
    14461 gacgagggcg gcggcaacgg gggaggcagg ggtaagggtt ccgggaacgg gaacgggaac
    14521 gggaacggga acgggaacgg gaacggcaac gggaacggca acgggaacgg gaacgggaac
    14581 gggaacggca acgggaacgg gaacgggaac gggaacggga acgggaacgg gaacggcaac
    14641 gggaacggca acacccgggc caaggccaag cgctagaggg agagagcgga tgaggggaat
    14701 cgaaccccca tctcgagctt gggaagctcg cgttctgcca ttgaactaca tccgcttgag
    14761 ggcccgagta tagcgactgc cgaggcgcgg ctctacgcgg aggatccgct cctctcggcc
    14821 atcgcctccg agggtccctg ctccccgaag atcgagttct cgaggacctg agccacgtcc
    14881 agcacctgca tgtcggagcc cttcgccttc gccccgtcgt cgagcatcac catgcagtag
    14941 gggcaggcca cgccgaggat gtccgctccc gtcgccgccg cctcgtcggt gcgctccatg
    15001 ttgacgcgct tgccgatcgg ctcctccatc cacatccgag caccgccggc gccacagcag
    15061 aagccgcgcg ccccgtggcg ctccatctcc accgtccgga gcccaggcac ggactccagc
    15121 gccgagcgag gctcgtcgta gacgccgttg tgccgaccga ggtagcacgg gtcgtgatag
    15181 gtgaggagcg cccgaacctc ctcggtgggg gtcagccggc cctgatccac gagccgggcc
    15241 agcagctcgg tgtggtggag aacctcgtag tcgcccccga agtccggata ctcgttccgc
    15301 agtgtgttga agcagtgagg gcaggcagcg acgatcttct tcgtcccgat gctgttcagc
    15361 gtctcgacgt tctcgcgggc aagctcctgg aagaggaact cgttccccat ccgacgcgcg
    15421 gggtcgccgt tgcacagctc acgaggcccc aggatcccga agcgcacgcc ggctttctgc
    15481 agcagcctcg tgaaggcccg agcgatcttc ctcgagcggt cgtcgaagga cgaggcgcag
    15541 ccgacccagt agaggtactc gggggcgtcg ccgtcctcaa cgatctgcac gccgagatcc
    15601 tcggcccagt ccgccctcga gctctgtgac tggccccagg ggttcttcga gctctccagg
    15661 tttcgcagga ggaggccggc ctcggtgggg aagctcgact cggccatcac gaggttgcgg
    15721 cgcatgtcca ggatgtggtc gacgtgctcg atgttgaccg ggcactcctg catgcacgcc
    15781 ccgcacgtcg tgcagtccca gacgacctcc tcctcgacga cgtcgggaac gagcgggacc
    15841 tgctcgtact gctcgcctcg cttcctcgcc tggaggatgt cgcccccgtg atcgaagagg
    15901 tgatcacgga ggttcatgat cagcagtttg gggttgagcg gcttccccgt gttccaggcc
    15961 gggcagacgt tctggcagcg gccgcactcg gtgcacgtgt aggtgtcgag catctgcttc
    16021 cacgtgaggt cctcgacggt ggcggcgccc agccgagact cctcgaggtt ctcgaggtcg
    16081 atgtcgatcg tctcgagctt ccccatcggc ttcgtcttgg agaagaagac gttgatcgag
    16141 ctggtgagga tgtggaggtg cttgctgaac gggatgtatg cgaggaagcc caggatcacg
    16201 gcgatgtggg accagaggaa gatctgacgg aagaggttct gcgcgtccct cgtcatcgtc
    16261 tcgaagagcg gtgcgatgaa gttcgagatt ggcgtccagg ccgccgggga ctcgttggct
    16321 cccatcgcga tcttcgtggc gttgaggaag aacagggtca ggatcacccc gaagatcagc
    16381 accaggatga agtcggcctc ctcgaggtgg ctgccgacga accggcggtc gcgacggacc
    16441 ttgcggaagt acagcgccat cccgatgccc acgagcacca gccccgcgaa caggtcttgg
    16501 acgaagccca gccacgcggt gcggccgacc caggggatag cgaagctctc gtcgaagatc
    16561 tcgccgccca cctcgatgat cgtggtcagc aggaccagga agccccagaa gatgaaggcg
    16621 tgcatcagcc ccggcccgat cctctgcagc agcttccgct ggccgaagac gacgacggct
    16681 tccttctcca gacggcgagg aaggtcctcg gtccgcttga cggggcggcc cagggtcacc
    16741 aagcggaaga ggaagtagcc gcgctgcatg aagatcccgc ccgcgagggc gaggagcccc
    16801 aggaggatga tgggtccgag cacgggccag agtctagggg ccggtcctgc ggcttcgcaa
    16861 cacaccccca tcaaggggag gggtcctcga accgataccc agagggtcgg atcgttacgg
    16921 caggtgccgt acacggaggg gtgaatgagc agacttcgaa gcgctctcgg gatcttcgtc
    16981 agcgtggcgg tgctcgtggc cgcgggcgtg gcgggtgccc gcggcacgtt cttcacctcg
    17041 gatcccgtgg acgaggggat ggaggaggtc gtcggggatc cggacccctg cgctcccgag
    17101 gagggcgaag gcgaggaggg cgagcccaag ggcgaaggcg aggagggcga gcccggggag
    17161 tgtgagcccg aggaggagcc cgaaggcgag gagctcgagg gcgacgacga gggcgagcaa
    17221 ggggaggccg aggtggggca gggcgagcgg gacgccgcct gcgacgcagc cgcggggatc
    17281 gaggagaccc ccctgccgga gggcgagaag ctgaccggtc tcgacaacgc gctctcccgc
    17341 gtcctcgaga actgcaagaa gaacccgcag gcgccggggc tgctcaacgc cctggagcgc
    17401 ctctccgaga accgggcgcg gaaggcggcc cgcgacgagg ccaaggccga gcggagggcc
    17461 gagcgtgagg cacgcaaggc cgagcggaag gcggcgaagg ggcagagcgg cggtcccccc
    17521 cagggtggtg gcccgccccc gcacgcggga cctcccgggg gcggagggcc tccgggccac
    17581 gccaagggag gcaaggggta aagggggttc cccggccgcc cgatagagag gttgggcgcg
    17641 gcggcgaccg tctggctggg tgggcgggcc ctgtcagagc ttcggtagcc gccgcgccca
    17701 ccgatccgaa gaagggccct ccggggccct tcttcctttg agccactctg cttgagcaac
    17761 attgacaata ggacgctcaa gtctctacca tgggccacgc atccgctgaa acccatcagg
    17821 agggcgtgac atggccagag cagtcggcat cgatctgggg acgacgaact cggtcgtctc
    17881 cgtgctggag ggcggcgagc ccaccgtcat ccccaacgcg gaggggtcgc ggacgacccc
    17941 ctccgtcgtg ggcttctcca agtccgggga gatcctcgtg ggtgaggtcg ccaagcgaca
    18001 ggcgatcacc aaccccgacc gcaccgttcg gtcggtgaag cgccacatgg gatccaagga
    18061 ctgggacgtc gagatcgacg acaagaggtg gacgccccag gagatctcgg cccagatcct
    18121 caccaagctg aagcgggacg cggaggccta cctcggcgac acggtcgtcc aggccgtgat
    18181 caccgtgccc gcgtacttcg acgacgccca gcgtcaggcc acgaaggagg ccggccagat
    18241 cgcgggcctc gaggtcctgc gcatcatcaa cgagcccacg gccgcatccc tcgcctacgg
    18301 cctggacaag gagcagcacg accagacgat cctcgtcttc gatctgggcg gcgggacctt
    18361 cgacgtctcg ctgctcgaga tcggggaggg cgtcttcgag gtgaagtcca cgagcgggaa
    18421 cacccagctc gggggcgacg actgggacca gcggatcatc gactggatgg tcaccgagtt
    18481 caagaacgcc cacggcgtcg acctctccag ggaccggatg gcgctccagc ggctccagga
    18541 ggccgcggag aaggcgaaga tcgagctctc tcaggtgacc gagacccaga tcaaccttcc
    18601 cttcgtcacc gccacgaacg aggggccgct ccacctcgac atgcggctca cccgctcgga
    18661 gttcgaacgg atgaccgagg acctcgtgca ggcggcccgc gagcccttcg agcaggccgt
    18721 gaaggactgg ggcaaggagg tctccgagat cgatcacatc atcctggtcg gcgggtccac
    18781 gcggatgccg atgatccagg agctcgtgag gggcctgacc gggggcaagg agccccacaa
    18841 gggcgtgaac ccggacgagg tggtggccgt cggcgcgggg atccaggccg gcgtgctcaa
    18901 gggcgaggtg aaggacgtcc tgctcctcga cgtcaccccg ctgtcgctgg gtatcgagac
    18961 caagggcggg atcttcacga agctgatcga gcggaacacg acgatcccga ccaagaagtc
    19021 ggagatcttc accacggccg acgacagcca aacgcaggtc gaggtgcacg tcctccaggg
    19081 cgagggcgag atggtggact cgcgagccaa ccggtcgctg ggccgattcc acctcaccgg
    19141 catcccgccg gcgccgcgcg gcatgcccca gatcgaggtg accttcgaca tcgacgcgaa
    19201 cggcatcgtc aacgtctcgg cgaaggacac cgcgacgggc aaggagcagg cgatgacgat
    19261 caccgggggg accgccctgg acagggacga gctcgaccgg atgatgaagg acgccgagca
    19321 gtacgcggag gaggatcacc ggcggcgcga ggccgccgag gcgcggaacc aggccgacaa
    19381 cctcgcctac caggccgaga agacggtgaa ggagctcggc gacaaggtgc ccgaggatga
    19441 tcggcaggtg gtggagtcgg cggtggccga cgtccgcaag gcgctcgagg gcgacgacct
    19501 cgacgagatc aaggcgaaga ccgaggccct gacggaagcc ttccagaagg tcggccaggc
    19561 cgtgtaccag cagcagcagg cggccgaggg cggagccgag caggaggccg ccgagggcga
    19621 ggaggacgtc gtcgaaggcg aggtcgtgga cgaagggggt agctcgtgac cgacgagcag
    19681 cggcccgatg acgtcgtcga ggaggtcgtg gaaggtgagg tcgaggaggc ccccgacacg
    19741 tcggagctcg acgaggctcg ggagcaggcg gccgagtacc tcgaccacct ccagcgcctc
    19801 aaggcggagt tcgagaacta ccgcaagcgg gtcctgaagg agcagacgcg cgctatcgag
    19861 atcgcctcgg aggacctcgt cctcaagctg ctgtccgtgc tcgacgactt cgagctcgcg
    19921 ctcgtggccg ccgagcagaa gcccgacttc gagaagttcc tccagggcgt ggagatggtc
    19981 tacgcgaagc tggtggacgt cctgcggtcc gagggcctgg agaagatcga ggcgaagggc
    20041 aagcccttcg atcccgagct ccacgaggca ctgttccagg gcggcgagga ggacggcgac
    20101 ccctacgtgg ccgacgtcct ccggaccggc tacacgctga aggggaag
//
LOCUS       DASUTW010000169         2352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_700073, whole genome shotgun sequence.
ACCESSION   DASUTW010000169 DASUTW010000000
VERSION     DASUTW010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700073"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..703
                     /locus_tag="VF058_05875"
     CDS             149..703
                     /locus_tag="VF058_05875"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX6207873.1"
                     /translation="MAEWMGTSWRVAGFVALSVVGIFAATLIGIRIAGRRTVSQMSAF
                     DFLVTVAIGTIVANTALVQRPSLLEGLVALVTLLLVQIGIALGRRKWAGLRRLVDLPP
                     IVLVRDGRTAERGMRVPHLTRDDMLERLRREGVYGLDEVALLVLEPTGEVTIVTDPSA
                     LRSEGLQILRDSAQNAEEADLPEP"
     gene            complement(826..1995)
                     /locus_tag="VF058_05880"
     CDS             complement(826..1995)
                     /locus_tag="VF058_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010310000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6207874.1"
                     /translation="MIELSEDQDAIVKAVREFVEQEVFPIADEMEHKDEFPEKIVEQL
                     KEMGLFGLTIPEEHGGAGMDVMTYALVVAELSRGWMSISGILNTHFMAAYLLNKHGTD
                     EQKERWLPRMTTGELRAAYSMSEAGAGSDVQAIKTKAERDGDHYVVNGSKMWVTNGLR
                     AGLVVTLVKTDPEADPPAKGMSLLYIEKEPGATEFEGITVPPNIPKMGYKGVETTELV
                     FENHRVPVENLLGEEEGQGFYQVMDGIEVGRVNVAGRAVGLATRAFEEAIKYAQEREA
                     FGKPIAHHQAIQFKLAQMGTKLEAARLMLLSAAQKKASGERSDLEAGMAKLFCSEACK
                     EIVEDALRIHGGYGYSAEYTVERLYREAPFLLIGEGTSEIQQMIIGRQLLERHKA"
     gene            complement(1992..>2352)
                     /locus_tag="VF058_05885"
     CDS             complement(1992..>2352)
                     /locus_tag="VF058_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017567865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX6207875.1"
                     /translation="GLQAIDGPYLLIKDLDGFREMAMRSRALGYDGKWALHPGQIDVL
                     NEVFAPTQEEYDKAEAVLEAYRHATEVEAMGAVMFGREMIDEASRKMAEQVAERGRAA
                     GLEATKTWDDFKEEWEQ"
BASE COUNT          320 a          850 c          763 g          419 t
ORIGIN      
        1 tcgaagtcct cgaagtaccg cccgaactct cgtgcctcgc ccatcgcgtc cctccttggt
       61 ctcgctggca tgctagcgga ccgacggtcg gcgcccggtt gaccgcgccg cccgcgggca
      121 tacgttccca ggagaggaag gagcacccat ggccgagtgg atgggaacga gctggagggt
      181 cgcaggcttc gtggcgctga gcgtcgtggg gatcttcgcg gcgacgctga tcggcatccg
      241 gatcgcggga cgccggaccg tctcacagat gtcggcgttc gacttcctgg tgacggtggc
      301 gatcggcacg atcgtggcca acacggcgct ggtgcagcgg ccgtcgctcc tcgaggggct
      361 cgtggccctc gtcacgctgc tgctggtcca gatcgggatc gcgctaggga ggaggaagtg
      421 ggccgggctg cgaaggctcg tggacctgcc cccgatcgtc ctcgtccgcg acgggaggac
      481 ggcggagcgc ggcatgcgcg tgccgcacct cacgcgcgac gacatgctcg agcgcctgcg
      541 ccgggagggg gtctatgggc tggacgaggt ggcgctgctg gtgctcgagc cgacgggcga
      601 ggtgacgatc gtcaccgatc cctcggcgct ccggtcggag gggctgcaga tcctgaggga
      661 cagcgctcag aacgccgagg aggccgacct gccggagccc tgagcggcgg cttccacgca
      721 gccggccgtc cccacgccga cccctcagag gaacatggcg acttcgccat atcttctaga
      781 cgggtcggcg tcgcacccgt cgaccgacga tccggccgcc gagcctcagg ccttgtgccg
      841 ctccagcagc tggcggccga tgatcatctg ctggatctcg ctggtcccct cgccgatcag
      901 caggaacggc gcctcgcggt agagccgctc cacggtgtac tccgccgagt agccgtaccc
      961 gccgtggatc cggagggcgt cctcgacgat ctccttgcac gcctccgaac agaacagctt
     1021 ggccatcccc gcctcgaggt cgctgcgctc ccccgacgcc ttcttctgtg ccgccgagag
     1081 cagcatcagc ctcgccgcct cgagcttcgt tcccatctgg gcgagcttga actggatcgc
     1141 ctggtggtga gcgatgggct tcccgaaggc ctcgcgctcc tgcgcgtact tgatcgcctc
     1201 ctcgaaggcc cgcgtggcca gccccacggc gcgccccgcc acgttcaccc gccccacctc
     1261 gatgccgtcc atcacctggt agaacccctg gccctcctcc tcgccgagga ggttctcgac
     1321 cggcacccgg tggttctcga agacgagctc ggtggtctcc acgcccttgt agcccatctt
     1381 cgggatgttg gggggcacgg tgatcccctc gaactcggtc gcgcccggct ccttctcgat
     1441 gtagaggagc gacatcccct tggcgggcgg gtcggcctcg gggtccgtct tcaccagcgt
     1501 caccacgagc cccgcgcgga ggccgttcgt cacccacatc ttcgagccgt tcacgacgta
     1561 gtggtcgccg tcgcgctcgg ccttcgtctt gatggcctgg acgtcggagc cggcgccggc
     1621 ctcggacatc gagtacgccg cgcggagctc gccggtggtc atcctgggca gccagcgctc
     1681 cttctgctcg tcggtcccgt gcttgttcag caggtacgcc gccatgaagt gggtgttgag
     1741 gatccccgag atgctcatcc atccgcgcga gagttccgcc acgaccagcg cgtaggtcat
     1801 cacgtccatc cccgccccgc cgtgctcctc ggggatcgtg agcccgaaga gccccatctc
     1861 cttcagctgc tcgacgatct tctcggggaa ctcgtccttg tgctccatct cgtcggcgat
     1921 cgggaagacc tcctgctcca cgaactcccg gaccgccttg acgatcgcgt cctggtcctc
     1981 gctgagctcg atcactgctc ccactcctcc ttgaaatcgt cccaggtctt cgtggcctcg
     2041 aggccggcgg cgcggccgcg ctcggcgacc tgctcggcca tcttccggct cgcctcgtcg
     2101 atcatctccc tcccgaacat gacggcgccc atggcctcga cctcggtggc gtggcggtag
     2161 gcctcgagca cggcctccgc cttgtcgtat tcctcctggg tgggggcgaa aacctcgttc
     2221 aggacgtcga tctgcccagg gtgcagggcc cacttgccgt cgtagcccag ggcccgcgag
     2281 cgcatcgcca tctcccggaa gccgtcgaga tccttgatca gcagataggg gccgtcgatc
     2341 gcctggaggc cg
//
LOCUS       DASUTW010000170         3315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1348151, whole genome shotgun sequence.
ACCESSION   DASUTW010000170 DASUTW010000000
VERSION     DASUTW010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348151"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="VF058_05890"
     CDS             <1..139
                     /locus_tag="VF058_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bL28 family ribosomal protein"
                     /protein_id="HEX6207876.1"
                     /translation="SHSHRRSPRRWSPNVQKVRVLVNGAPRRLHVCTKCLKAGKVQRA
                     V"
     gene            complement(136..927)
                     /gene="thiD"
                     /locus_tag="VF058_05895"
     CDS             complement(136..927)
                     /gene="thiD"
                     /locus_tag="VF058_05895"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019889751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX6207877.1"
                     /translation="MAEVRTALTIAGSDSGGGAGIQADLKTFTALGVFGTTAITAVTV
                     QNTKGVEGYEELSPETVGGQIRAVATDIGVHAAKTGMLASAAIIEAVADALVETRVPN
                     LVVDPVFVSKHGHPLLQDDAVEALKARILPLATLVTPNLPEAAGLTGLQVEAEQDLRR
                     AGEAILELGAGAVLVKGGHLSGPEATDLLVTGDGATPFSSPRVDTPHTHGTGCVLSAA
                     IAARLALGDDLEVAVRGGKEFVTGAIRGALEIGEGIGPVDPAWRS"
     gene            complement(920..1915)
                     /gene="thiL"
                     /locus_tag="VF058_05900"
     CDS             complement(920..1915)
                     /gene="thiL"
                     /locus_tag="VF058_05900"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX6207878.1"
                     /translation="MATEDELVRAIKKLLSGEEPGVVVPIGDDAAVVEPGGHHLVVTV
                     DTLVERVHFDPDVAQPDDLGYKAVVVNVSDVAAMGGSPRYAVVSLAVPPDTRPAWVIE
                     LYGGMRAAAEDYGLALVGGDTVRADQCVLTVTVLGTVAEGRAVTRSGARPGDRIVVTG
                     SLGAAAGGLRLVQGREGATRRAVMTGPGRELVAALDRPVARVGEGEALAQAGATSMID
                     VSDGLALDLSRVCEASGVGARVRLPAVPVAEALKELAEEIGVDPLDLALHGGEDYELV
                     ATLPAAAVEDARARLLDRYGTPLTDIGEIIETGLLAVGEDGRERPLEAKGWDHFG"
     gene            2004..2213
                     /locus_tag="VF058_05905"
     CDS             2004..2213
                     /locus_tag="VF058_05905"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX6207879.1"
                     /translation="MPVINTLDLEGVSEKSWGDAAREALKEAAKTIRHINRLEVLDYG
                     TEVEDEKIARYRTHVRIYFQVESER"
     gene            2318..>3315
                     /locus_tag="VF058_05910"
     CDS             2318..>3315
                     /locus_tag="VF058_05910"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF013461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEX6207880.1"
                     /translation="MIEAKRVHDNPSPEELRAFTEEMPSCRITEYDNPNVQTRAVSRS
                     AASTYVATDDPSITDSQTITREEYARIAAMQDAYIREQEMVVIDGFIGNDPGFRTRAR
                     LVIEKANANIAGMQQKLYYPPEADGEPEVTVIYTPNLPAPGYPDDRLIAVDLEAGVTR
                     VLNSDYFGESKKGGLRMWNKLVYDRGGLSLHAGLKMIPVDGERRVGLIIGLSGTGKTT
                     TTFTNQNDSLPAQDDFIALMPGGRVYGTENGCFAKTFSLDPDFEPAIFGAITRPTAYL
                     ENVYQDDDGRVNFFETSYTQNGRAVFGLEDLGAYKDPRDLAKVDFMLILNRNENIIP"
BASE COUNT          523 a         1261 c         1084 g          447 t
ORIGIN      
        1 gagccactcc catcggcgct ccccgcgccg gtggagcccg aacgtgcaga aggttcgggt
       61 gctcgtgaac ggcgctcccc gccggctcca cgtgtgcacg aagtgcctca aggccggcaa
      121 ggtccagcgc gccgtctagg aacgccaggc cgggtccacc ggcccgatcc cctcgccgat
      181 ctccagcgcc ccgcggatcg cccccgtgac gaactcctta ccgccgcgca ccgccacctc
      241 gaggtcgtcg cccagcgcga gccgcgcggc gatcgccgcc gagagcacgc agccggtccc
      301 gtgggtgtgc ggcgtgtcga cccgcgggct cgagaacggc gtcgcaccgt cgccggtgac
      361 caggaggtcg gtggcctcgg gcccggagag gtgcccgccc ttgacgagca ccgcccccgc
      421 gccgagctcg aggatcgctt cgcccgcccg gcgcaggtcc tgctcggcct cgacctggag
      481 cccggtgagg ccggccgcct cggggaggtt cggcgtgacc agcgtggcca gggggaggat
      541 ccgagccttc agcgcctcga cggcatcgtc ctggagcagg ggatgcccgt gcttggagac
      601 gaagacaggg tccacaacga ggttgggcac ccgcgtctcg acgagggcgt cggccaccgc
      661 ctcgatgatc gcggccgacg ccagcatccc ggtcttggcc gcgtgcacgc cgatgtcggt
      721 ggccaccgcc ctgatctggc cgcccaccgt ctcgggcgac agctcctcgt agccctcgac
      781 ccccttcgtg ttctggaccg tgacggccgt gatcgcggtc gtcccgaaga ccccgagggc
      841 cgtgaacgtc ttgaggtctg cctggatccc ggccccgcct cccgagtccg agccggcgat
      901 cgtcagcgcc gtgcgaacct cagccaaagt gatcccaccc cttcgcctcc agcggccgct
      961 ctcggccatc ctcccccaca gcgagcaggc ccgtctcgat gatctccccg atgtcggtca
     1021 ggggcgtccc gtaccgatcg agcagcctcg ctcgcgcgtc ctccacggcc gcggccggga
     1081 gcgtcgccac cagctcgtag tcctctcccc cgtgaagggc gaggtccagc gggtccacgc
     1141 cgatctcctc ggccagctcc ttcagcgcct ccgcgaccgg cacggcgggc agcctcaccc
     1201 gggccccgac cccgctcgcc tcgcacaccc gcgagaggtc gagcgccagc ccgtcggaga
     1261 cgtcgatcat cgaggtggcc ccggcctgcg ccagtgcctc gccttcgccc accctggcca
     1321 ccggacggtc gagggccgcc acgagctcgc ggcccggacc cgtcatgacc gcgcgcctgg
     1381 tcgcgccctc gcggccctgc acgagccgca ggccgccggc cgccgcgccc aggctccccg
     1441 tgaccacgat ccggtccccc ggccgggctc ccgacctggt cacggcccgc ccctcggcca
     1501 cggtccccag gaccgtcacc gtcaggacgc actggtccgc ccggaccgtg tcccccccga
     1561 cgagggccag tccatagtcc tcggccgccg cccgcatgcc cccgtagagc tcgatcaccc
     1621 acgccggcct ggtatcgggc ggaacggcga gcgagacgac cgcgtagcgg gggctcccgc
     1681 ccatggccgc gacgtcggag acgttcacga ccacggcctt gtagccgagg tcgtcgggct
     1741 gggccacgtc cggatcgaag tggacgcgct ctacgagcgt gtccaccgtc acgaccagat
     1801 ggtgcccccc cggctcgacg accgcggcgt cgtccccgat agggacgacg acccccggct
     1861 cctcacccga gagcagcttc ttgatggcgc ggaccagctc gtcctctgtg gccacggctt
     1921 cccttcctcg gtccctctcg gggataatcc tagaggcgcg ggcccgctcc ggcccgccga
     1981 ggaccgtcga gaggagacga gacatgccgg tgatcaacac gctggatctc gagggtgtct
     2041 ccgagaagtc gtggggcgac gccgcccgcg aggcgctgaa ggaggcggcg aagacgatcc
     2101 ggcacatcaa ccgcctcgag gtgctcgact acggcaccga ggtggaggac gagaagatcg
     2161 cgcgctaccg gacccacgtg cgcatctact tccaggtcga gtccgagcgg tagttcgagg
     2221 agcgccccga gcgggtacac ccccggagcg cgtcctgggg gggcccgctc cgctgggtgt
     2281 accatgggcg tccccgacat cgccgtagga ggaggccgtg atcgaggcga agagggtcca
     2341 cgacaatcca tcgcccgagg agctccgcgc gttcaccgaa gagatgccca gctgccggat
     2401 cacggagtac gacaacccga acgtgcagac ccgcgccgtc tcgcgcagcg ccgccagcac
     2461 ctacgtcgct accgacgacc cctcgatcac cgactctcaa acgatcaccc gcgaggagta
     2521 cgcgcgcatc gcggcgatgc aggacgcgta catccgcgag caggagatgg tcgtgatcga
     2581 cggcttcatc gggaacgacc cggggttccg cacgagggcc aggctggtga tcgagaaggc
     2641 caacgccaac atcgcgggga tgcagcagaa gctctactac ccgccggagg ctgacggcga
     2701 gcccgaggtc accgtgatct acacaccgaa cctccccgcc cccggctacc ccgacgatcg
     2761 cctcatcgcg gtggatctcg aggcgggcgt cacccgcgtc ctcaactcgg actacttcgg
     2821 ggagtcgaag aagggcgggc tgcggatgtg gaacaagctc gtctacgacc gcggcggcct
     2881 ctcgctccac gcgggcctca agatgatccc ggtggacggg gagcggcgcg tgggcctcat
     2941 catcgggctc tccgggaccg gcaagaccac gacgacgttc accaaccaga acgactccct
     3001 gcccgcccag gacgacttca tcgcgctgat gccgggcgga cgggtctacg gcaccgagaa
     3061 cgggtgcttc gccaagacgt tctccctgga tcccgacttc gagccggcga tcttcggggc
     3121 catcaccagg cccaccgcct acctggagaa cgtctaccag gacgatgacg gcagggtgaa
     3181 cttcttcgag acctcctaca cgcagaacgg acgggcggtc ttcggtctcg aggacctggg
     3241 ggcctacaag gacccccggg acctggccaa ggtggacttc atgctgatcc tgaaccgcaa
     3301 cgagaacatc atccc
//
LOCUS       DASUTW010000171         7317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1607866, whole genome shotgun sequence.
ACCESSION   DASUTW010000171 DASUTW010000000
VERSION     DASUTW010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607866"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VF058_05915"
     CDS             complement(<1..219)
                     /locus_tag="VF058_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020519402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6207881.1"
                     /translation="MTEQELARGAARRLAIIRHAQEVTGNVSKTCRYYGISRQAYYKW
                     LRRYEEHGIEGLRDRSRGPHNCPHATKAE"
     gene            complement(1066..5094)
                     /locus_tag="VF058_05920"
     CDS             complement(1066..5094)
                     /locus_tag="VF058_05920"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX6207882.1"
                     /translation="MEFRLLGPLEVSDGERRLPLGGPKQRTVLAHLLVRANEVVTVDR
                     LIDGTWGDEPPGTARNTIQAYVHNLRKALGAERIQHRSSGYVLTADASEVDALRFEAL
                     VGKAHRAAAADPSGAVEALREALRLWRGPALDDLSEQPALQPEIARLEELRMAALEER
                     IGAELALGDHRALIPELETLIRRHPFREGLWGHLMVALYRGGRQGDALAAYRRAREFL
                     ADELGVDPSPELRRLHEQILRQDPGLELTGEPLRGYRLLELLGEGAFGAVHRAFQPEV
                     GREVAVKIIRPEFANHPEFIRRFASEAQLVARLEHPHITPVYDYWREPGGAYLVMRYL
                     RGGSLREALAHGPLEADRVVQVMDQIASALAAAHRQGVVHRDVKPANILFDEEANAYL
                     SDFGIATDLAAAEATEQGRTTSPFASYLSPEEARGEAATPRADIYGLGVVLYEALGGR
                     HPFAETPPDALAAAHAREPFPSIRSVRPDLPPALEEVIGRAAAKDPEARYEDARMLAS
                     AVRDALVAGRAQPVTLPPLKARNPYKGLRPFLEADAVDFFGREALTERLLDRMREGGV
                     GSKFLAVVGPSGSGKSSVVRAGLLPALRHGALPGSDRWFVATMHPGSHPLEELEAALT
                     RIAVDPPPGLADRIERDESGLLEAAEEVLPPDASVFLVVIDQFEEAFTLVGEEERRKF
                     LATLLAAVTDPRSRVRVIVTLRADFYDRPLAYRGFGDLLAGRTQAIAPLARDELERVV
                     SGPAEGVGVAVEPRLAAEIVAEVSDQPGGLPLLQYALTELFERRRDATLTLEAHHEIG
                     GVSGALARRAEDLYGRLTQAGKEAARQLFLRLVSPGEERSGDTRERALRAELTTLGVD
                     REALEAVIDTFGTHRLLSFDRDPATRGPTVEVAHETLLSEWDRLARWIDRHRADLRRR
                     HSLAAAAGEWEAHGRDPDYLPTGSRLAEFEEWGRTTTLELTDGERRFLDASLQRRRRE
                     QARKVERLERQRRLERRARTRLWALAAAIVLLAGAATYGGLALLGGRPPDTVLVFQGG
                     GDEGFNDMIEAGFDRAVTRLDIEAGKIVPQAVSFDTALRRVAEAGARLIVVAGTEDIG
                     AIAGDFPDTRFIALDSSGDRPNVSYLRFAEHEGSFLVGAAAALKSETGTIGFVGGVDY
                     ELIWRFQAGYEAGARAIDPEIQVLSRYLTEPPDFSGFSSPTLGAQAASELFTAGADVI
                     YHAAGWSGVGVFLAAAARSGEEGEHLWGIGVDTDQYRSLPVLQESVGDEGPPLEPHVL
                     TSMVKRVDHAIYTALGDYSRDTFIPGVREFDLAAGGVDIAYSGGFIDDIRPVIEDLRQ
                     QIIAGEIDVPALPVDKAGAPAP"
     gene            5237..5731
                     /locus_tag="VF058_05925"
     CDS             5237..5731
                     /locus_tag="VF058_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207883.1"
                     /translation="MATYVQRGAPGIGRRTLVVGLTAMVVGGVAGGMLAWMLQLPRLE
                     VVSRAPALSPGQVADGLRYQGLAEQFAIARGQIAWTQRYQGLADEMLAERATIARGRI
                     AWGERYQGLADQLSGHELTRGQVAHGQRYTALAEEILGPGLTRAQIAWAERYQALADQ
                     LAGG"
     gene            complement(7178..7250)
                     /locus_tag="VF058_05930"
     tRNA            complement(7178..7250)
                     /locus_tag="VF058_05930"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7215..7217),aa:Arg,seq:cct)
BASE COUNT         1082 a         2548 c         2566 g         1121 t
ORIGIN      
        1 ttcggccttg gtggcgtgag ggcagttgtg cggtcccctc gagcggtccc tgagcccctc
       61 gatcccgtgc tcctcgtagc ggcgcagcca cttgtagtag gcctgccggc tgatcccgta
      121 gtagcggcac gtcttggaga cgttgcccgt cacctcctgc gcatgacgga tgatcgccaa
      181 ccgccgagcc gcccctctag ccagctcctg ttcggtcact tggcctcctc ccggtccgga
      241 ggtccaagtg tcaaccatgt caggcagttt cagacaagga tcagtcggaa cacctgtcaa
      301 ccatcagccg gagcggtgtc aagcctcagg tgacgccgga acgtcgagca tcacccgaaa
      361 cacgacaatc acatagcgcc cccggggcga accgaacgaa gggggagacg accaagccgg
      421 tgtattcgtg gaggtcaggg caacgtactc gctctccggg ccgaggcgga agcactgttg
      481 aagggtccgc tgataatcca tgatctccgg gtgattcgcg aggggcggtt cgctgcttct
      541 ccatgaccat cacgcgtgcg attctggacc gcggagccgc accgataggc atgcgaacct
      601 aagcaagcgg gccgtcggat ctggatcgcc ccccgctcgg gggggcgagt ctcctgaccg
      661 gcgcctctgt gccaactcca cgatttctct acgccttggt gccccgggcc acgtctctca
      721 tccgaagctt cacgatgtct ccacggtgcc gatcgtcggc cgaacttcgt gtcggcgagt
      781 tccgggacgc cgggctcgtc gagcgggaga tgctcgagag gccccgggtg gcgccacggt
      841 gtatccggct gaccacggcc ggccgcgagg tggagccgcc ggctctgcgc gccttcggcg
      901 actgggccga acgcgctgct gcggtcacgc gagcccacga gagttcaccc taatcgcagt
      961 gccccccggg agtgtctggt tcagtagtgc accgctgccg gggcagcatc cgctgtccgc
     1021 aagtgcttcc ttgcgagccg caacgtttcg cccggtggct ctgcgttacg gggccggggc
     1081 cccggccttg tctactggaa gggcggggac gtcgatctcg cccgcgatga tctgttgccg
     1141 caggtcctcg ataaccgggc ggatgtcgtc gatgaatccg ccgctgtagg cgatgtccac
     1201 gccgcccgcg gccaggtcga actcacgcac acccgggatg aaggtgtctc gcgagtaatc
     1261 ccccagggct gtgtagatcg cgtggtcgac cctcttcacc atcgacgtga ggacgtgggg
     1321 ctccaaaggc ggaccctcgt ctccgacgga ttcctggagg acgggaagcg agcggtactg
     1381 atcggtatcg accccgatcc cccataggtg ctcgccttcc tctccagagc gggccgcggc
     1441 ggccaggaac acgccaactc ccgaccagcc cgccgcgtgg tagataacgt ccgcgcccgc
     1501 tgtgaacagc tcgctcgccg cctgcgcccc cagcgtcggg ctgctgaacc cgctgaagtc
     1561 cggcggctcg gtcaggtagc gggacagcac ctggatctcg gggtcgatcg ccctggcgcc
     1621 cgcctcgtag ccggcctgga accgccagat gagctcgtaa tcgacgccgc cgacgaagcc
     1681 gatggtcccg gtctcgctct tcaatgccgc cgccgcgccg acgaggaacg agccctcgtg
     1741 ctcggcgaac ctgaggtagg agacgttggg gcggtcgccg gaggagtcaa gggcgatgaa
     1801 ccgcgtatcg ggaaagtcgc cagcgatcgc cccaatgtcc tctgtcccag ccaccacgat
     1861 cagtcgggcc ccggcctccg cgacccttcg caaggcagta tcgaaggaga cagcctgcgg
     1921 gacgatcttc ccagcctcga tgtcgagtct cgtcaccgcc cggtcgaacc cggcctcgat
     1981 catgtcgttg aagccctcat cgcctcctcc ctggaagacg agcacggtgt cggggggacg
     2041 gcccccgagc agggccagcc ccccataggt ggcggccccg gccagcaaga caatggcggc
     2101 cgccagggcc cagagcctgg ttcgcgccct ccgctcgagc cgccgctgcc gctcgaggcg
     2161 ttcgaccttc cgagcttgct cccggcgacg gcgctggagg gaggcatcga ggaaccggcg
     2221 ttcgccgtcg gtgagctcga gcgtcgtcgt ccgcccccat tcctcgaact ccgcgagccg
     2281 gctccccgtc gggaggtagt cgggatcgcg gccgtgagcc tcccactcgc ccgccgccgc
     2341 agcgagggag tggcggcggc ggaggtcggc ccggtggcgg tcgatccacc gggcgagccg
     2401 gtcccactcc gacaggagcg tctcgtgggc gacctcgacc gtcgggccgc gcgtggcggg
     2461 gtcccggtcg aaggacagca gccggtgggt cccgaaggta tcgatcaccg cctccagggc
     2521 ctcgcggtcc accccgaggg tggtgagctc cgctcggagg gccctctctc gggtgtcccc
     2581 gctccgctcc tctcccgggc tcaccaggcg gaggaagagc tgacgcgcgg cctccttccc
     2641 cgcctgggtc agcctcccgt agagatcctc ggcgcggcgg gcgagggctc ccgagacccc
     2701 accgatctcg tggtgggcct ccagggtgag cgtcgcgtcc ctccgccgct cgaacagctc
     2761 ggtgagggcg tactggagga gagggaggcc accgggctgg tcggagacct cggccacgat
     2821 ctccgcggcc agccggggct cgacggcgac gccgacgccc tcggcgggtc cggacaccac
     2881 ccgctccagc tcgtcgcggg cgaggggggc gatcgcctgg gtcctccctg ccagcaggtc
     2941 cccgaatccc cggtaggcca ggggtcggtc gtagaagtcg gcccggagcg tcacgatgac
     3001 ccggacccgg ctccgcgggt cggtcacggc ggcgaggagg gtcgccagga actttcttcg
     3061 ctcctcctcg cccacgaggg tgaaggcctc ctcgaactgg tcgatcacga cgaggaagac
     3121 cgaggcgtcg gggggcagga cctcctcggc tgcctccagc agcccgctct cgtcccgctc
     3181 gatccggtcg gcgagcccgg gaggcggatc gaccgcgatg cgcgtgaggg cggcctccag
     3241 ctcctcgagg gggtgggagc cggggtgcat cgtggccacg aaccagcggt cggagcccgg
     3301 cagcgctccg tgtcgcagcg cagggagcag gccggctcgg acgacggagg acttgccgct
     3361 accggacggc ccgaccacgg cgaggaactt cgagcccact cccccctccc gcattcggtc
     3421 gaggagccgt tcggtcagcg cctcccggcc gaagaagtcg acggcgtcgg cctcgaggaa
     3481 cggtcgcagg cccttgtagg ggttgcgggc cttgagcgga gggagcgtca ccggctgggc
     3541 gcgcccggcg accagggcgt cacgcacggc gctcgcgagc atcctcgcgt cctcgtagcg
     3601 cgcctctggg tccttggcgg cggcccggcc gatcacctcc tcgagggccg ggggaaggtc
     3661 cggccgaacc gagcggatgg agggaaaggg ctcgcgggcg tgcgccgcgg cgagcgcgtc
     3721 gggcggggtc tcggcgaacg ggtgccgccc gcccagggcc tcgtagagga cgacccccag
     3781 gccgtagatg tccgcccgtg gggtcgcggc ctcacctcgc gcctcctccg gggacaggta
     3841 ggaggcgaag gggctggtgg tccgaccctg ctccgtggcc tcggccgccg ccaggtccgt
     3901 ggcgatcccg aaatcggaga gataggcgtt ggcctcctcg tcgaagagga tgttggccgg
     3961 cttgacgtcg cggtggacca cgccctggcg gtgggctgcg gcgagcgccg aggcgatctg
     4021 gtccatcact tgcacgaccc ggtccgcctc gagcggcccg tgcgccaggg cctcccggag
     4081 gcttcctcct cgcaggtagc gcatcacgag gtacgcaccg ccgggctccc gccagtagtc
     4141 gtagacgggg gtgatgtggg gatgttcgag gcgggccacc agctgggcct ccgaggcgaa
     4201 gcggcggatg aactcggggt ggttggcgaa ctcgggccgg atgatcttga cggcgacctc
     4261 tcttcccacc tcgggctgga aggctcggtg gacggccccg aaggcaccct ccccgaggag
     4321 ctcgaggagc cggtacccgc gcaggggctc gcccgtgagt tccagcccgg ggtcctggcg
     4381 gaggatctgc tcgtgaagac gccggagctc gggggaaggg tcgaccccga gctcgtcggc
     4441 gaggaactcc cgggcccgcc ggtaggcagc cagggcatcc ccctggcggc ccccgcgata
     4501 cagggccacc atcaggtggc cccacagccc ctcgcggaac gggtggcgcc tgatgagggt
     4561 ctcgagctcg gggatcaggg cgcggtggtc ccccagagcg agctcggcgc cgatccgttc
     4621 ctcgagcgcc gccatccgga gctcctccag gcgggcgatc tccggctgaa gagccggctg
     4681 ctccgagagg tcgtccagcg ccggtcccct ccagaggcgg agcgcctctc gtagggcctc
     4741 gacggcccct gaggggtcgg ctgccgccgc acggtgtgcc tttcccacga gggcttcgaa
     4801 gcgcagcgcg tccacctcgc tcgcgtcggc ggtgaggaca tagcccgacg agcggtgctg
     4861 gatccgctcc gcgccgagcg ccttgcgaag gttgtggacg taggcctgga tcgtgttccg
     4921 ggcggtcccg ggcggctcgt ctccccacgt cccgtcgatg aggcgatcga cggttaccac
     4981 ctcgttggcc ctgaccagga ggtgggcgag taccgtgcgt tgcttgggtc ccccgagggg
     5041 aagcctccgc tccccatcgg agacctcgag gggtccgagt agccggaatt ccatgccccg
     5101 acggtacgcc ggggctggct cgaatggaat agccggtttc agggttcttt cagcctctcc
     5161 ctggggggct ttcaacgctc gcctcgatcc tggccccgag agccctcacg gggagggcgc
     5221 gagaaaggag gccaaaatgg ccacgtatgt ccagcggggg gctcccggga tcgggcgccg
     5281 gacgcttgtc gtgggcctca ccgcgatggt cgtgggaggc gtcgccgggg ggatgctcgc
     5341 ctggatgctc cagttgccga ggctggaggt cgtctcacgg gcgccagcgc tgtcgccggg
     5401 gcaggtcgcc gacggcctgc gctaccaggg gctcgcggag cagtttgcga tcgcccgggg
     5461 gcagatcgcc tggacccagc ggtaccaggg tctcgctgac gagatgctag cggagcgggc
     5521 cacgatcgcc aggggacgga tcgcttgggg cgagcgctac cagggactcg ccgaccagct
     5581 ctcgggtcac gagctgacca ggggccaggt cgcccacggg cagcgctaca cggcgctcgc
     5641 cgaggagatc cttggacctg ggctgaccag ggcgcagatc gcctgggcgg agcggtacca
     5701 ggccctcgcc gaccagctcg cgggcggctg agccgcggct cggggccggt cgccgtcggg
     5761 ccgtgatgcg tcgaggtgga cgcgaagccg ggcgtctctc actcgagggt cgcctggctt
     5821 cacggtttct tagaaggtac tgccattcga cggggcctgc ccccaaaatg acctgctctt
     5881 ctacccggac ttcctaacgt ggtggggaga cacgctggtc gccgcggccg agggcgtggg
     5941 gtgacggagg gcggagggca aggccctggt cgccgcaacc cccccgactg gcgggccgtc
     6001 cctgaggccc cagccagccg gagggaagcc tgaagggcgc aaaactccat ccgtcatgcc
     6061 gcttcgatct cgtactcgtg gatcaggtcg ccgagcacat cccttcgacg aatccgcggt
     6121 gcgggctcgg tgagcacggg agtctcccca ctttcgggcg tcgcgaggtc gatcccccga
     6181 tggggtctgg cccggctgta atgctctaac gtactcgccg agcacccaat cgaggtgccg
     6241 ccacgacagg atcagcaggt ggtccaggca ctcgtcgcgc acggttcgca cccaccgctc
     6301 cgcgaatgcg ttcgctttgg gggcccggat cggcgtctcg gatcgctttt gtcccctccg
     6361 cccggaacac ctcgtcgaag ggacccgaga agcgagcgct ccggtggctg ttggtggggg
     6421 cgtcggaggg gcaccggatc gagatcgggt gcgccgggcc acggccggac ggggagcacg
     6481 ctcgccggcc tgctcgtcct ccaggggatg agtgctcact cggctttgcg gcaaatccgg
     6541 cgctcgtact gccacgacgc cgtggagtcg ctggagcagt ccacaatgct ccggcgtctt
     6601 gttccgtagc cctggcccgg acccggacgg gatcgtccgg gtccgggctc ccatcttcga
     6661 atcggaaccc cggccgatcg ctctccccta tcccttgcga tggccgagtg cggcgcttgc
     6721 ccgggcgtcc gcctcagcgt tccactcccg agggatccac gagatctgtg tatcagtgaa
     6781 ctcctcaaga agcgcccggg cttccacgtt tagggcttcg tactcagccg tggctcccag
     6841 gcgccgctga tctcgtagcg agcgatccag gtgtggacgc tctggcggga cactcccagg
     6901 cgccgggcca ccccaccacc ctccacccgt cctggaccac cgccatcacc gattgatacc
     6961 gctgctccat gacactgagc tccaccagca cggcccacct ccttccctcg acccaagggg
     7021 ccgagaatag gagacgggcc aggccgccac tgtcaggcat catccggagc caccgtcagg
     7081 gatcaggcgg aacacctgtc aaccatcagc cggagcggtg tcaagcctca ggtgacgccg
     7141 gaacgtcgag catcacccga aacacgacat cacatagcgc ccccgggagg aatcgaacct
     7201 ccgacacccg gtttaggaaa ccggtgctct atccactgag ctacgggggc gggggcgatc
     7261 ctccccaagt ctagcgggtg ggaaagccac gcccaggcgg ggcggcatcc ctcggcc
//
LOCUS       DASUTW010000172         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_313395, whole genome shotgun sequence.
ACCESSION   DASUTW010000172 DASUTW010000000
VERSION     DASUTW010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313395"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VF058_05935"
     CDS             complement(<1..666)
                     /locus_tag="VF058_05935"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6207884.1"
                     /translation="MKIAEAIGSALAKLEVRDAFGLLGSGNFLVTQAMVDRGVRFVAA
                     RHESAAVSMADGYARVSGRPGVVTVHQGPGLTNAMTALTEAAKARTPLLVLAADTSAG
                     ASRSNFAVDQAGLATAVGAVAERARSPETALEDLARAWRTAVVERRAVLFSMPLDVQA
                     GVASGDEIEAPEAPEPAAPAHEDVERVADLLAAAASPVLVGGRGAVLAGAGPAIEALG
                     NRVG"
     gene            complement(663..1154)
                     /locus_tag="VF058_05940"
     CDS             complement(663..1154)
                     /locus_tag="VF058_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX6207885.1"
                     /translation="MEEPVVERPDGLKHLSPLYAGAFVGLLRAAEGLEARLSVRLERE
                     HGLSLRGFEVLLFLSVFAPEGELRMSDLAEQTPLSQSRVSRLVADLEARGLVRRIPAE
                     SDGRGVGVSVTKEGIERFRAAQESHLADLEELLFSNLSETEVRRLAAITGKIVEACRE
                     GER"
     gene            1248..1682
                     /locus_tag="VF058_05945"
     CDS             1248..1682
                     /locus_tag="VF058_05945"
                     /inference="COORDINATES: protein motif:HMM:NF039977.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6069 family protein"
                     /protein_id="HEX6207886.1"
                     /translation="MDTIERSSGAAARAAARPQRRRRLAAVGTAVALATATWVLIEPV
                     AGVDLRAPGQGSPGFDIGLVAVILTAAGAALLGWGLLAAMERWTRRPRAWTWVALGGF
                     LASLTGPMSGTGIPGSHRWLLALLHLVVAASLIPLMRRTIDG"
     gene            1686..2111
                     /locus_tag="VF058_05950"
     CDS             1686..2111
                     /locus_tag="VF058_05950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX6207887.1"
                     /translation="MAGFSDAELEYLTTERRLARIATADADGRPHVTPVGMWRYNPAL
                     GTVDVTGRDFSSTRKYRNVGANPRAAIVVDDVASMRPWRPRAVIVEGPATAVDGHEPL
                     IRITPERVISWGLDAGPGRDERGGGAMQEGSGSGAKRKG"
BASE COUNT          290 a          827 c          753 g          272 t
ORIGIN      
        1 cccgacccgg ttgccgagcg cctcgatcgc gggacccgcc ccggccagca ccgcgccgcg
       61 cccgcccacg aggacgggtg aggcggcggc ggccagcagg tcggcgaccc gctccacgtc
      121 ctcgtgggcg ggcgcggccg gctccggcgc ctccggcgcc tcgatctcat ctcccgatgc
      181 cacaccggcc tggacgtcca gcggcatgct gaacaggacc gcgcgccgct ccaccaccgc
      241 agtccgccac gcccgcgcca gatcctcgag cgcggtctcg ggcgaccggg cccgctcggc
      301 caccgcgccc accgccgtgg ccagcccggc ctgatccacc gcgaagttcg accgggaagc
      361 cccggccgaa gtatcggccg cgagcacgag caggggggtc cgcgccttcg ccgcctcggt
      421 cagggccgtc atcgcgttgg tgagccccgg gccctgatgg acggtcacga cgcccggccg
      481 gccgctcacc cgcgcgtacc cgtcggccat gctcaccgca gcgctctcgt gccgggccgc
      541 cacgaagcgc acgcctcgat ccaccatggc ctgcgtcacg aggaagttcc cgctccccag
      601 gaggccgaag gcgtcacgga cctccagctt ggccagggcc gacccgatcg cctcggcgat
      661 cttcaccgct ccccctctcg gcaggcctcg acgatcttgc cggtgatggc ggcgaggcgc
      721 cggacctccg tctcgctgag gttggagaac agcagctcct cgaggtccgc caggtggctc
      781 tcctgggcgg cccggaagcg ctcgatgccc tccttcgtca ccgacacgcc gaccccccgt
      841 ccgtcgctct cggcggggat tcgccggacc aggccccggg cctccagatc ggccacgagc
      901 ctcgacactc gactctggct cagaggggtc tgctcggcga ggtcgctcat ccggagctcc
      961 ccctcggggg cgaagacgga gaggaacagc agcacctcga acccgcgcag gctgagcccg
     1021 tgctcccgct cgagccggac cgagagacgg gcctcgagcc cctcggccgc ccggagtagc
     1081 ccgacgaagg cccccgcgta gaggggcgag aggtgcttca ggccatccgg ccgctccacg
     1141 acgggctctt ccatcgccac ccgccgatcc tagcgtgaga ccacttgcgt acgcaagcat
     1201 tcgcctatat gcttgcgcat acatagccat ttggaaagga ggccgtcatg gacaccatcg
     1261 agaggagcag cggggcggcg gccagggcgg cggcccggcc tcagaggaga cggcggctcg
     1321 ccgcggtggg cacggccgtc gccctagcca ccgccacctg ggtcctgatc gaaccggtcg
     1381 cgggcgtcga cctccgcgcg cccggccaag ggtccccggg cttcgacatc gggctggtcg
     1441 ccgtgatcct cacggccgcc ggtgccgcgc tcctcggctg gggcctgctc gccgcgatgg
     1501 agcggtggac ccgtcgcccc cgggcctgga cctgggtcgc gctgggaggc ttcctggcct
     1561 cgctcacggg accgatgtcc ggcacgggga tcccggggag ccaccgctgg ctcctggcgc
     1621 tcctccacct cgtggtggcc gcgtcgctca tcccgctgat gcgccgcacg atcgacgggt
     1681 gacccgtggc cgggttctcc gacgcagagc tcgagtacct gacgacggag cggaggctgg
     1741 cccgcatcgc caccgccgac gccgacgggc gccctcacgt cacgcccgtc ggcatgtggc
     1801 gctacaaccc cgctctgggc acggtcgacg tcaccggccg cgacttctcg tccacccgca
     1861 agtaccggaa cgtgggggcg aatcctcggg ccgcgatcgt cgtggacgac gtcgcgtcga
     1921 tgcgtccctg gcgccctcgt gcggtgatcg tggagggccc ggcgacggcg gtcgacgggc
     1981 acgagcccct catcaggatc acccccgagc gggtcatctc ctggggcctc gatgccggcc
     2041 cgggccggga cgagcgaggg ggcggcgcga tgcaagaggg ctcgggcagc ggggcgaagc
     2101 gaaagggatg agccgggacg agcgaggggg cgagcggccg gg
//
LOCUS       DASUTW010000173         7474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_314051, whole genome shotgun sequence.
ACCESSION   DASUTW010000173 DASUTW010000000
VERSION     DASUTW010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7474
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314051"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..677)
                     /locus_tag="VF058_05955"
     CDS             complement(18..677)
                     /locus_tag="VF058_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6207888.1"
                     /translation="MNILIVEDEERIASFVEKGLKANGFTTSVAHDGEAALLLHRTGD
                     FDLVILDLGLPGMDGLEVLRELRGRDTRLSVIILTARDEAPDVVAGLDLGADDYVTKP
                     FRFDELQARVRARLRTDREPETTVIRAGDCALDLRTRRVTVEDRTIELTAREFALAET
                     FFRHPGQVLSREQLLSNVWGYDYDPGSNVVDVYVGYLRKKLGGDRIVTVRGMGYRLEA
                     G"
     gene            complement(674..2143)
                     /locus_tag="VF058_05960"
     CDS             complement(674..2143)
                     /locus_tag="VF058_05960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6207889.1"
                     /translation="MTEERAAATTREADAPEPRSRRILWGVRARMLTWYIVVLTVAIG
                     ASVLVARQVLIAQLDERIDRDLVQESEELRRLSGGRDPSTGEPFRTNVRRIFEVFLQR
                     NIPARNETFLTIVGGEPFERSFREPPYRLDRDPELVERWSGLQRADRGRVETPAGDVE
                     YLAVPVRSGGRTRGVFVAAIFRDLELEDIQQAIWGTVGVGLVTLVIGSILAWRVIGGV
                     LGRVKRVTDTARDFSDDLTRRLHVTGNDEVSHLALTFNGMLDRLEDLMETQRRFVDDA
                     GHELRTPITVIRGHLELLDDDPEERERTLALVTDELDRMQRIVNDLLILAKAERPDFL
                     DLEPVPLDRLTEEIHAKAKALGERDWRLQESAAGIVIGDRQRLTQAVMQLAQNAVQNT
                     QTGSEIGIGSSMQNGEARLWVRDTGPGIPPDQRDRLFARFSRGVGRRRSEGAGLGLAI
                     VRAIAEAHHGRVEVESPPGSGATVSVVIPVDQPEEGRDA"
     gene            complement(2109..2441)
                     /locus_tag="VF058_05965"
     CDS             complement(2109..2441)
                     /locus_tag="VF058_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207890.1"
                     /translation="MSLRRVLSVLLLLALGFGVGWALVDLTRPGIRDEEVPAIIVREE
                     EDDEPPPGGGGSGGDGGSGGGGGSGGGGWGGGGGASPAPAPAPAPAGDDSDDHGTDDG
                     GAGGGDDT"
     gene            2575..3465
                     /locus_tag="VF058_05970"
     CDS             2575..3465
                     /locus_tag="VF058_05970"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX6207891.1"
                     /translation="MQDRTKESWDFAEGEEIVPGLHALDLLGGGWRYEVYLAWDERLL
                     TVVAVKLLRPDRVGDRRAMERFGVEAEALQRLQHPVLPRCFGVDLDGEHPHLVLELMD
                     GPRLSSLIRKQKALAIEQVIPLGLQLSSALHYMGTEGIVHLDVKPKNVIMGAPPRLID
                     LSIAAPLERARRIEGPIGTDAYMAPEQCEEGPHPRIEHRTDVWGLGVTLYEAIAGRLP
                     YPRGEEDAEGDARFPQLTVEPAPLPREIPSVLTDPVMACIRHDPQERPTAAELAEALQ
                     PLADALPRSPRLGRRRPKIM"
     gene            3546..3989
                     /locus_tag="VF058_05975"
     CDS             3546..3989
                     /locus_tag="VF058_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207892.1"
                     /translation="MSGDDAADGRRKEDDMLAKVILAAIAIALGGAVAWVTGPGDELA
                     PVSGVEIQDEAKRPDDGDDDDDDDRGRGGDGDNTRGNDGTSGGNNTGDGDDTRGNDGT
                     SGGNNTVRAATATNTNGSGGGGGGTNTGGGTNTGGGSDSGGGGDT"
     gene            complement(4042..4686)
                     /locus_tag="VF058_05980"
     CDS             complement(4042..4686)
                     /locus_tag="VF058_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011318730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6207893.1"
                     /translation="MEAPSERTRVRRHHERGVYDRETIHAILDEALFCHLAVIVDGAP
                     RAVPTIHARIDETLYVHGSNASRTLRALKDGGEACTVVTLLDGLVLSRSAFGHSMNYR
                     SVVVYGVPREVTDPDEKWEAQRALVDHVIPGRSADTRMPNEKELRQTTILALPLDEAS
                     AKVRTGPPKDEDDDLGLPFWAGEVPLRIVAGDPVPDPSLPEGIEAPPYARRYRR"
     gene            4786..6237
                     /locus_tag="VF058_05985"
     CDS             4786..6237
                     /locus_tag="VF058_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEX6207894.1"
                     /translation="MTTTFAARYRAIASRDPRHDGRFFTAVTSTGIYCRPSCPARTPR
                     PENVRFFPTAQAARSAGFRPCLRCEPDRWTPSDAGGTSEDLARRALALIMGGIVDEEG
                     VGGLSARLHVSDRHLHRLVTASFGSPPLAIARARRAEAAWDLLTLTDMRASDVAFAAG
                     YASVRQFNDHIRETFGSSPTELRRRGGGGGGVRSLVLRLRHRRPYAGEPVLAFLEARA
                     IAGLEEREHGAYRRSLRLGRSHAVLEGTLNPEEGHVTVRLELGDLRDLPRAVSAARRV
                     FDLDADPEAVATALSADPALRPLVRRRPGMRIPGHPDGLELAVRAIVGQQVTVAGART
                     LVGRLVSELGEALPSPTGGVTHLFPSAEALLGADLSSIGLTTGRIRAIQALAEAVTSG
                     RLKLDAGTPPRDVQRSLLELPGIGPWTAGYVAMRLLDPDAIPVDDLGLRRAYASLRGS
                     GEPLMARAEAWRPWRAYAAMHLWTHLAQRNSAL"
     gene            complement(6216..7226)
                     /locus_tag="VF058_05990"
     CDS             complement(6216..7226)
                     /locus_tag="VF058_05990"
                     /EC_number="3.5.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013004572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008660 -
                     1-aminocyclopropane-1-carboxylate deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0042217 - 1-aminocyclopropane-1-carboxylate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-aminocyclopropane-1-carboxylate deaminase"
                     /protein_id="HEX6207895.1"
                     /translation="MTLDDFERFPLMFGPSPIHRLERLTEHLGGADLWAKREDCNSGL
                     AFGGNKVRKLEYLAADAIAGGCDTLVSIGGVQSNHTRQVAATAARLGMKCVLIQESWV
                     EWPDAVYDRVGNILLSRLMGADVRLMEAGFGIGFKESWERAISEIEEAGGKPYAIPAG
                     ASDHPLGGLGFARWAREVAEQERELDVFFDTIVVCSVTGSTQAGMVAGFAALDEERRR
                     RVIGIDGSAKPKETQEQVARIARNTAELIEARELTDDEIVLDDRYHAGTYGIPDEQTL
                     DAMRLAARLEGMITDPVYEGKSMAGMIDLVDTGEIPKGSTVLYAHLGGQPALSAYSAL
                     FR"
     gene            complement(7243..7404)
                     /locus_tag="VF058_05995"
     CDS             complement(7243..7404)
                     /locus_tag="VF058_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207896.1"
                     /translation="MLERRLSRWVMVFVLVVLSSLGVPAVLSTARPNRERDDTAAVLV
                     IGEDLARLG"
BASE COUNT         1058 a         2793 c         2557 g         1066 t
ORIGIN      
        1 gctcgacgca cccgccctca gccggcctcg agacggtagc ccatgccccg cacggtcacg
       61 atccgatcgc cccccagctt cttccgcagg tagccgacgt agacgtccac cacgttggag
      121 ccggggtcgt agtcgtaccc ccacacgttg ctcagcagct gctccctgct gaggacctgc
      181 ccggggtgcc ggaagaaggt ctcggcgagg gcgaactccc gcgccgtcag ctcgatcgtg
      241 cggtcctcga ccgtcacccg ccgcgtccgc aggtcgagcg cgcagtcccc cgcccggatc
      301 acggtcgtct cgggctcccg gtcggtccgg agacgcgccc ggacccgggc ctgaagctcg
      361 tcgaaccgga agggtttcgt gacgtagtcg tcggcgccca ggtcgagccc ggccacgacg
      421 tccggcgcct cgtcccgggc ggtcaggatg atcaccgaga ggcgcgtgtc ccggccgcgg
      481 agctctctca gcacctccag gccgtccatc cccggcagcc ccaggtcgag gatgacgagg
      541 tcgaagtccc cggtccggtg gagcaagagc gccgcctccc cgtcgtgggc gaccgacgtc
      601 gtgaacccgt tcgccttcag ccccttctcc acgaacgagg cgatccgctc ctcgtcctcg
      661 acgatgagga tgttcacgcg tccctccctt cctccggctg gtccacgggg atcacgacgg
      721 agaccgtggc ccccgatccc ggcgggctct cgacctccac cctcccgtgg tgggcctccg
      781 cgatcgcccg gacgatcgcc agcccgaggc ccgcgccctc cgagcggcgt ctccccacgc
      841 cccgcgagaa gcgggcgaag aggcggtcgc gctggtcggg ggggatcccc ggacccgtgt
      901 cgcgcaccca caacctcgcc tcgccgttct gcatcgagga gccgatcccg atctccgagc
      961 ccgtctgagt gttctggacg gcgttctggg ccagctgcat cacggcctgg gtcagccgct
     1021 gacgatcccc gatcacgatg cccgctgcgg actcctggag ccgccagtcc cgctccccga
     1081 gggccttcgc cttggcgtgg atctcctcag tgaggcggtc cagcggcacc ggctccaggt
     1141 cgaggaagtc gggccgctcg gccttcgcca ggatcagcag gtcgttgacg atgcgctgca
     1201 tgcggtcgag ctcgtccgta acgagggcca gcgtgcgctc ccgctcctcg gggtcgtcgt
     1261 ccagcagctc gaggtgaccc cggatcacgg tgatcggcgt gcggagctcg tggcctgcgt
     1321 cgtccacgaa ccggcgctgc gtctccatca ggtcctccag gcggtccagc atcccgttga
     1381 acgtgagcgc caggtgcgag acctcgtcgt tccccgtgac gtgcagccgg cgcgtcagat
     1441 cgtccgagaa gtcccgggcc gtgtcggtca cccgcttcac acgcccgagg accccgccga
     1501 tcacgcgcca ggccaggatc gagccgatca cgagcgtgac gaggcccacc cccaccgtgc
     1561 cccagatcgc ctgctggatg tcctccagct ccaaatcacg gaagatcgcc gccacgaaga
     1621 cgcctctcgt cctccccccc gagcgcaccg gcaccgcgag gtactcgacg tcgcccgccg
     1681 gcgtctccac ccggccgcgg tcggctcgct ggagcccgga ccagcgctcc accagctcgg
     1741 gatcgcggtc cagccggtag ggcggctccc ggaagctccg ctcgaagggc tcgcctccca
     1801 cgatcgtgag gaaggtctcg ttccgagcgg ggatgttccg ctggaggaac acctcgaaga
     1861 tccgccggac gttggtgcgg aacggctccc ccgtgctcgg gtcccggcct cccgagagcc
     1921 tccgcagctc ctcgctctcc tggaccagat cccggtcgat ccgctcgtcg agctgcgcga
     1981 tcaggacctg gcgggccacg aggacgctcg cgccgatcgc caccgtcagc accacgatgt
     2041 accaggtgag catccgggca cggacgcccc acaggatcct gcggctccgg ggctcagggg
     2101 cgtccgcctc acgtgtcgtc gccgccgccc gctcctccgt catcggtgcc gtggtcgtcc
     2161 gagtcgtctc ccgccggagc gggagcgggc gcaggggccg gggacgcgcc tccgcctccg
     2221 ccccagccgc ctccgcccga gccgcctccc ccgcccgagc cgccatcccc gcccgagcca
     2281 cctccgcccg gagggggctc gtcgtcctcc tcctctcgga cgatgatcgc cgggacctcc
     2341 tcgtcccgga tccccggacg ggtcaggtcg accagcgccc accccacccc gaagcccagg
     2401 gcgaggagca ggagcacgga cagcacacgt cgaaggctca tggttcgaag gatggcggtc
     2461 gcgcatcagc cctccatgag aggcccatga ggatgttctc atggagtctt catacccggc
     2521 tcacggcccc gggcgatggt tgtctcggat caaggtgggg aggatgcgat ggacgtgcag
     2581 gaccggacca aggagagctg ggacttcgcg gaaggcgagg agatcgtccc cggcctccac
     2641 gcgctcgatc tgctcggcgg cggctggcgc tacgaggtgt acctcgcctg ggacgagcgg
     2701 ctgctgaccg tcgtggcggt gaagctgctg aggccggacc gggtgggcga ccgaagggcg
     2761 atggagcgct tcggcgttga ggccgaggcg ctgcagcggc tccagcatcc cgtgctcccc
     2821 cggtgcttcg gtgtggacct cgacggggag cacccgcatc tcgtcctcga gctgatggac
     2881 ggtccgcgcc tctccagcct gatccggaag cagaaggcgc tcgccatcga gcaggtcata
     2941 ccgctcgggc tgcagctcag ctccgcgctc cactacatgg gcacggaggg catcgtccac
     3001 ctggacgtca agccgaagaa cgtgatcatg ggagcgcctc ccaggctcat cgacctcagc
     3061 atcgcggcgc ctctggagcg ggcgaggcgg atcgaggggc cgatcggaac cgacgcctac
     3121 atggcccccg agcagtgcga ggaggggccg cacccccgga tcgagcaccg caccgacgtg
     3181 tgggggctcg gggtgacgct gtacgaggcg atcgccggtc gcctccccta cccgaggggc
     3241 gaggaggacg ccgaggggga cgcgcggttc ccccagctca ccgtggagcc ggcgccgctg
     3301 ccccgggaga tcccgtcggt gctcacggat ccggtgatgg cgtgcatccg gcacgatccc
     3361 caggagcgcc ccaccgcggc cgagctcgcc gaggccctcc agccgctcgc cgacgcgctg
     3421 cccaggagcc cccgcctcgg acgacggcgc ccgaagatca tgtgagaggg gcagcggcct
     3481 caagtgagac ctctctcacg gagggctcat ggtggcctca ggaccgggcg ggaggatagg
     3541 taccagtgag cggcgatgac gccgcagacg gacgacggaa ggaggacgac atgttggcga
     3601 aggtgatcct cgcagccatc gcgatcgccc tcggtggcgc cgtcgcgtgg gtgaccggcc
     3661 cgggcgacga gctggccccc gtctccggcg ttgagatcca ggacgaggcg aagcgcccgg
     3721 acgacggcga cgatgatgac gacgacgacc gcgggcgagg tggcgatggc gacaacaccc
     3781 gcgggaacga cggcacctcc ggtgggaaca acaccgggga cggcgacgac acccgcggga
     3841 acgacggcac ctcgggcggg aacaatacag tccgcgcggc caccgcgacc aacacgaacg
     3901 gcagcggtgg cggcggtggt ggcaccaaca ccggcggcgg aacgaacacc ggcgggggtt
     3961 cggacagcgg cggcggcggc gacacctgag tcgcaccgat cgtcgaactg aaggggcgcc
     4021 tccgggcgcc ccttctccgt ctcagcggcg gtagcggcgc gcgtacggcg gcgcctcgat
     4081 cccctctggc agcgaggggt cgggcacggg gtcaccggcg acgatccgca ggggcacctc
     4141 accggcccag aacggcaggc ccaggtcgtc gtcctcgtcc ttgggcggcc cggtccgcac
     4201 cttggcggag gcctcgtcga gcggcagggc caggatcgtc gtctgccgga gctccttctc
     4261 gttcggcatc cgcgtgtccg ccgaccgtcc cgggatcacg tgatccacga gcgcgcgctg
     4321 agcctcccac ttctcgtccg ggtcggtgac ctcgcggggg accccgtaca ccaccaccga
     4381 gcggtagttc atggagtggc cgaaggccga gcgcgagagg acgagcccgt cgagcagcgt
     4441 gacgaccgtg caggcctcgc ctccgtcctt cagggcccgc agcgtgcggc tggcgttgga
     4501 gccgtggacg tacagcgtct cgtcgatccg tgcgtggatc gtggggacgg cccggggagc
     4561 cccgtccacg attaccgcca ggtggcagaa gagcgcctcg tcgaggatcg cgtggatcgt
     4621 ctcgcggtcg tagacgccgc gctcgtggtg cctgcggacc ctcgtccgct cgctgggagc
     4681 ctccatgtcg agggtgatgg tagcgggtct cgacgatcgt ccccgccgat cggcgatgtc
     4741 cgaaagccgc cagcgctcgt cgggcggagc ggcgatgatg ctcgcatgac gacgaccttc
     4801 gccgctcggt accgggccat cgccagccgc gaccctcggc acgacggtcg cttcttcacc
     4861 gccgtcacca gcacgggcat ctactgccgg ccgagctgcc ccgctcggac ccccaggccc
     4921 gagaacgtcc ggttcttccc cacggcccag gcggcccgct cggccgggtt ccgtccgtgc
     4981 ctccgctgcg agcccgaccg atggacaccc tcggacgccg gtgggacctc agaggatctc
     5041 gccaggcggg ccctcgccct gatcatgggc ggcatcgtgg acgaggaggg ggtgggtggg
     5101 ctctcggccc gcctgcacgt cagcgaccgg cacctgcatc gcctggtgac cgcgagcttc
     5161 gggtctcctc ccctcgccat cgccagagcc aggcgcgcgg aggcggcttg ggatctcctg
     5221 accctgaccg acatgcgggc gtccgacgtc gccttcgcgg ccggatacgc cagcgtccgg
     5281 cagttcaacg accacatcag ggagaccttc ggctcctccc ccaccgagct caggaggcga
     5341 gggggcggag gaggcggcgt tcgctcgctc gtcctccgcc tgcggcatcg tcggccgtac
     5401 gcgggggagc cggtgctcgc gttcctcgag gcccgcgcca tcgcgggcct cgaggaacgc
     5461 gagcacggcg cgtaccggcg gagcctcagg ctgggtcgct cccacgccgt cctcgaaggg
     5521 acgctcaatc cggaggaggg ccatgtcacc gtccggctcg agctcgggga cctgcgcgat
     5581 ctccctcggg ccgtgagcgc ggcgaggcga gtcttcgatc tcgacgccga tcccgaggcc
     5641 gtggcgaccg cgctctccgc ggatccggcc ctcaggccgc tggtgcgccg gcggccgggg
     5701 atgcggatcc ccggacaccc cgacgggctc gagctcgccg ttcgtgcgat cgtcggccag
     5761 caggtcaccg tggcgggcgc ccggaccctc gtcgggcgcc tcgtgtcgga gctcggcgag
     5821 gccctcccgt cccccaccgg cggggtcacg catctcttcc cgagcgccga ggcgctcctc
     5881 ggagccgacc tctcctcgat cgggctcacg acggggcgca tccgggcgat ccaagcgctg
     5941 gccgaggccg tgacgagcgg ccggctgaag ctcgacgcgg ggacccctcc ccgcgacgtc
     6001 cagcgatcgc tcctcgagct gcccggcatc ggcccctgga cggccggcta cgtggcgatg
     6061 cggctcctcg acccggacgc gatcccggtg gacgacctcg ggctgcgccg ggcttacgcc
     6121 tcgctccgag gctcaggtga gccgctgatg gcccgcgcgg aggcctggag gccgtggcga
     6181 gcctacgccg cgatgcacct gtggacccac ctcgctcagc ggaacagcgc gctgtaggcg
     6241 ctgagcgccg gctgtccccc gaggtgcgcg tacagcacgg tggagccctt cgggatctcg
     6301 cccgtgtcca cgaggtcgat catccccgcc atcgacttcc cctcgtagac ggggtcggtg
     6361 atcatcccct cgagccgggc ggcgagccgc atcgcgtcga gcgtctgctc gtcggggatc
     6421 ccgtacgtcc ccgcgtggta gcggtcgtcg aggacgatct cgtcgtcggt cagctcccgg
     6481 gcctcgatca gctcggcggt gttccgcgcg atccgggcga cctgctcctg ggtctccttg
     6541 ggcttggccg agccgtcgat cccgatcacg cgtcgccggc gctcctcgtc cagcgcggcg
     6601 aacccggcga ccatgcccgc ctgcgtgctc cccgtgaccg agcagacgac gatcgtgtcg
     6661 aagaacacgt cgagctcacg ctcctgctcg gccacctcgc gggcccacct cgcgaacccc
     6721 aggccgccca gggggtggtc ggaggcgccg gccgggatcg cgtacggctt gccgccggcc
     6781 tcctcgatct cggagatcgc ccgctcccag ctctccttga acccgatccc gaatcccgcc
     6841 tccatcagcc ggacatccgc acccatcaac cggctgagca ggatgttccc cacccgatcg
     6901 tagacggcgt ccggccactc cacccagctc tcctggatca gcacgcactt catcccgagg
     6961 cgcgccgcgg tggctgcgac ctggcgcgtg tggttcgact gcacgccacc gatcgagacg
     7021 agcgtgtcgc atcccccggc gatcgcgtcg gcggcgaggt actcgagctt ccgcaccttg
     7081 ttcccgccga aggccaggcc gctgttgcag tcctcgcgct tggcccagag gtccgctcct
     7141 cccaggtgct cggtgagccg ctccagccga tggatcggag agggcccgaa catcagcggg
     7201 aagcgttcga agtcatcgag cgtcatggcg tcctcctgcg gctcatccga gccgggccag
     7261 atcctccccg atcacaagca cggcggcggt gtcgtcgcgc tcccgattgg gccgggccgt
     7321 cgacagcacg gcgggcaccc cgagcgagga gagcaccacg agcacgaaca ccatcaccca
     7381 tcgcgagaga cgtcgttcga gcacggcacc catcctatcc gcggcggccg atgcggccca
     7441 ggtgcgtgct tccgaacccc tccgggtact tcag
//
LOCUS       DASUTW010000174         2943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1609157, whole genome shotgun sequence.
ACCESSION   DASUTW010000174 DASUTW010000000
VERSION     DASUTW010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609157"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1413
                     /locus_tag="VF058_06000"
     CDS             <1..1413
                     /locus_tag="VF058_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6207897.1"
                     /translation="PPQIVSELDLPRHGYRVSLIDPDYYVPYEDGTSLTLWGDVGRTV
                     EEIAKYSKRDAEAYVEFDRSFERLSQLLRDMLFVVPPNLTMRELPRWLSVARKLRGWS
                     GKDIAELVRLFTISGADFLDEWFEDERVKGALATQTIIGAWCGPMSPGSAYVLMHHWI
                     GEVDGHDGAWGWVHGGMGGVSEALASAAREAGAEIRTGMPVRSVVTRNGEALGVELED
                     GTKLRARRVISGAHPVTTYLDLVGEEHLPDEVVRDVRRYRSRSGSVKVNLGLSELPRP
                     KAWEGPIPGDPHTGIMAISPSVEYLERAWDDAKYGRPSRDPYIEAVFPTAHEPGIAPE
                     GKHVALCFTQFGPFDLREGSWDTERAAYGRRVVEVLDAHCPGFADSVEHMEVLTPPDI
                     EERFGLLGGNIFQGDMTPDQLFSFRPIPGYGDHRSPIRGLYLCGSGTHPGGGVMAVPG
                     RNCAGVVRADHRRARWGGRR"
     gene            1410..2576
                     /locus_tag="VF058_06005"
     CDS             1410..2576
                     /locus_tag="VF058_06005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6207898.1"
                     /translation="MRLSDEDLAIRERARTLADELISFEVEAEMAGGSLPPDVRERHR
                     ARAEELGLTAISAPAELGGRGFTVLQQVLAQEQIGRVTNALGWVVTTPPTWLPKVATE
                     HQMETWVLPTIRGERHECYAITEEGAGSDVDAIEARARRDGDEYVLTGVKWHVTSFNK
                     ADHVFFQAKLEGGENAGTHAMFIVDTDSPGVRVVRTPRYSHSYPDEHPIVAFEDVRVP
                     AANLIGEEGDGMSFTHEWFRYERLMIAARCCGAADRLIEEAEGFARERVAFGQPIAEY
                     QGVAFPLADSIVELWAARLMTYRTAREMDEGADVKLLHAHCSMAKLYASEMANRVADR
                     AVQIFGGRGYMRENVAERSFRELRVDRIWEGTSEVQRAIIADQLGKRGPGIAEG"
     gene            2581..>2943
                     /locus_tag="VF058_06010"
     CDS             2581..>2943
                     /locus_tag="VF058_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013732275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6207899.1"
                     /translation="MEWRIEVVFVPVSNVDRAKAFYRDAVGFHEDFDTRFTDDLRMVQ
                     LTPPGSGCSIALMSGAAAGRPGPPDIEPGSLQGLMVVVDDLTAARERLLEHGVEVSEI
                     LEVVHEEGGSVYRPVAGEP"
BASE COUNT          430 a         1001 c         1099 g          413 t
ORIGIN      
        1 ccgccccaga tcgtctccga gctcgacctc ccccgccacg ggtaccgcgt ctcgctgatc
       61 gaccccgact actacgtccc ctacgaggac ggcacctcgc tgacgctctg gggcgacgtc
      121 gggcgcaccg tggaggagat cgcgaagtac tcgaagcgcg acgccgaggc ctacgtcgag
      181 ttcgaccgct ccttcgagcg gctgtcccag ctgctccgcg acatgctgtt cgtcgtgcct
      241 cccaacctca cgatgcgcga gctgccgcgg tggctctcgg tcgcccggaa gctgcggggc
      301 tggtcgggga aggacatcgc cgagctcgtc cgcctcttca cgatcagcgg cgccgacttc
      361 ctcgacgagt ggttcgagga cgagcgggtg aagggcgcgc tggccaccca gacgatcatc
      421 ggggcctggt gcggccccat gagccccggt tccgcctacg tcctgatgca ccactggatc
      481 ggagaggtcg acggccacga cggcgcgtgg ggatgggtcc acggcggcat gggcggcgtg
      541 agcgaggcct tggcgagcgc ggcgcgcgag gcgggggccg agatccggac ggggatgccc
      601 gtccggagcg tggtgacgcg gaacggcgag gcgctcggcg tggagctcga ggacgggacg
      661 aagctccggg cccgccgcgt gatctcgggc gcccaccccg tcaccaccta cttggacctc
      721 gtgggcgagg agcacctccc cgacgaggtc gtgagggacg ttcgccgcta ccgcagccgg
      781 tcgggctcgg tgaaggtcaa cctcgggctg tccgagctgc cgaggcccaa ggcctgggag
      841 ggccccatcc cgggcgatcc ccataccggg atcatggcga tctcgccctc ggtggagtac
      901 ctggagcggg cctgggacga cgccaagtac ggccgaccct cgcgcgaccc gtacatcgag
      961 gcggtgttcc ccacggccca cgagcccggg atcgccccgg aggggaagca cgtcgcgctg
     1021 tgcttcacgc agttcgggcc cttcgatctc cgcgagggct cctgggacac cgagcgcgcg
     1081 gcctacgggc gccgggtcgt cgaggtcctc gacgcccact gccccgggtt cgccgactcg
     1141 gtggagcaca tggaggtgct gaccccgccc gacatcgagg agcgcttcgg gctcctgggg
     1201 ggcaacatct tccagggcga catgacgccg gaccaactct tctcgttccg gccgatcccc
     1261 gggtacgggg accaccgctc gccgatccgg gggctgtacc tctgcgggtc ggggacccac
     1321 cccgggggcg gcgtgatggc ggtgccgggg cgcaactgcg ccggcgtggt gcgcgccgac
     1381 caccgacgcg cgcgctgggg tgggcggcgg tgaggctctc ggacgaggac ctcgcgatcc
     1441 gcgagcgagc ccggacgctg gccgacgagc tgatctcgtt cgaggtggag gccgagatgg
     1501 ccggcggttc gctcccgccg gacgtccggg agcgccaccg ggccagggcc gaggagctcg
     1561 ggctgaccgc gatcagcgcc ccggccgagc tcgggggccg aggcttcacc gtgctccagc
     1621 aggtcctggc ccaggagcag atcgggcgag tgacgaacgc gctcggatgg gtggtcacca
     1681 cgcctcccac ctggctcccc aaggtcgcca ccgagcacca gatggagacc tgggtcctcc
     1741 ccacgatccg cggggagcgc cacgagtgct acgcgatcac cgaggaggga gccggctccg
     1801 acgtggatgc gatcgaggcc cgggcccgca gggatggcga cgagtacgtc ctcaccggcg
     1861 tgaagtggca tgtgacctcg ttcaacaagg ccgaccacgt cttcttccag gccaagctgg
     1921 agggaggaga gaacgcgggg acccacgcga tgttcatcgt ggacacggac tcgccgggtg
     1981 tccgggtcgt gcgcacgcct cgctacagcc actcgtaccc cgacgagcac ccgatcgtgg
     2041 ccttcgagga cgtgcgggtc cccgccgcca acctgatcgg ggaggagggc gacggcatgt
     2101 ccttcaccca cgaatggttc cgctacgagc gcctcatgat cgcggcccgc tgctgcgggg
     2161 cggcggaccg cctgatcgag gaggctgagg ggttcgcgcg ggagcgcgtg gccttcgggc
     2221 agcccatcgc cgagtaccag ggcgtggctt tcccactcgc ggactcgatc gtggagctgt
     2281 gggccgcgcg cctgatgacc taccgcacgg cgagggagat ggacgagggc gccgacgtga
     2341 agctgctgca cgcccactgc tcgatggcca agctctacgc gtccgagatg gcgaaccggg
     2401 tggccgaccg ggccgtgcag atcttcgggg gccgcggcta catgcgcgag aacgtggccg
     2461 agcggtcctt ccgggagctg cgggtggacc ggatctggga ggggacctcg gaggtccagc
     2521 gggccatcat cgccgaccag ctgggcaagc gcgggccggg catcgcggag gggtgaaggg
     2581 gtggagtggc ggatcgaggt cgtgttcgtc cccgtgtcca acgtggaccg agccaaggcg
     2641 ttctaccggg acgcggtcgg gttccacgag gacttcgaca ctcgattcac cgatgacctg
     2701 cggatggtcc agctgactcc tcccggctcc ggatgctcga tcgcgctgat gtcgggggcg
     2761 gcggcggggc gacccgggcc gcccgacatc gagccgggct cgctccaggg actgatggtc
     2821 gtcgtcgacg atctcaccgc ggcgcgggag cgcctcctcg agcacggcgt cgaggtcagc
     2881 gaaatcctgg aggtcgtcca cgaggagggc ggctcggtct accgcccggt ggcgggggag
     2941 ccg
//
LOCUS       DASUTW010000175         2624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_962914, whole genome shotgun sequence.
ACCESSION   DASUTW010000175 DASUTW010000000
VERSION     DASUTW010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962914"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VF058_06015"
     CDS             <1..314
                     /locus_tag="VF058_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX6207900.1"
                     /translation="RANNGIRPLKMGEKLRRKAHRHSVRMSKQRRIFHHSCLSCIAPG
                     SWTRIGENVAKAKTVRAAHRALMRSSGHRSNILCGCYSKAGMGVVKRAGWVYVTQIFL
                     G"
     gene            335..1276
                     /gene="meaB"
                     /locus_tag="VF058_06020"
     CDS             335..1276
                     /gene="meaB"
                     /locus_tag="VF058_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017620894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX6207901.1"
                     /translation="MTLADGVLSGDRRSIARAISMVEDGSEGLRDLMAGLFPRTGRAL
                     TIGLTGSPGVGKSSLAAVLVEGARKRDRSVAVLAIDPTSPFTGGALLGDRLRMQEHAT
                     DPGVFIRSMATRGHLGGLALAAPEAVRVLDASGVDLVVIETVGVGQAEVEVATEADTT
                     LVVVSPGWGDAVQASKAGILEIADVFVVNKADREGARETARDLEQMLHLGPELPWPPP
                     VVLTSTVNGEGMEELWEAIEGHQKHLESTGELDRRRRARLLREVEGIVAERLRVRAQS
                     LLERGDLEALADDLAARRTDPYAAADILVGRLDEEAR"
     gene            1273..>2624
                     /locus_tag="VF058_06025"
     CDS             1273..>2624
                     /locus_tag="VF058_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017615214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX6207902.1"
                     /translation="MNKEEWRQRYRQAGERDADFETISGEPIEPLYTPEDLEGFDPDR
                     DLGFPGEYPYTRGVYPSMYRGRLWTMRQFAGFGSPKQTNERYKYLLKHGQGGLSVAFD
                     MPTLMGRDSDDPHAEGEVGRCGVATDSLADFEILFDGIPLDDITTSMTISGPAAILFA
                     FFLAAAEKQGVDWTTLDGTLQTDILKEYIAQKEWIFPPRPHLRIIGDLMEFCADEVPQ
                     WHPISVSGYHIREAGSTAVQELAFTLADGFSYVELGKERGLDVDRFSQGLSFFFNAHI
                     DFFEEIAKFRAARRIWARWMKERYGAEDPQSWRLRFHTQTAGCSLTAQQPENNIVRTA
                     IEALAGVLGGTQSLHTNALDEVLALPTEKAAQIALRTQQVIAHETGVANVIDPLGGSY
                     FVEALTNKAEEQAEEYFRKIEEMGSGSILEGMLVGIERGYFQSEIADAAFREQERYEK
                     G"
BASE COUNT          416 a          882 c          939 g          387 t
ORIGIN      
        1 ctcgtgcgaa caacgggatc cggcccctca agatgggcga gaagctgcgg cggaaggccc
       61 accgccacag cgtccggatg tcgaagcagc gccggatctt ccaccacagc tgtctcagct
      121 gcatcgcccc cggcagctgg accaggatcg gcgagaacgt ggccaaggcg aagacggtcc
      181 gggccgcgca cagggcgctg atgcggagct cgggtcaccg gtccaacatc ctctgcggct
      241 gctactcgaa ggcgggcatg ggtgtggtga agcgtgccgg ctgggtctac gtgacccaga
      301 tcttcctggg ctgaccgact gctacgctgt ccgcatgacc ctcgcggacg gcgtcctctc
      361 cggcgatcgg cgctcgatcg ctcgcgccat ctccatggtc gaggacggga gcgaggggct
      421 gcgcgatctg atggccggcc tcttcccccg cacgggacgt gccctcacga tcgggctgac
      481 gggctcgccg ggcgtgggga agtcgagcct ggccgcggtg ctcgtcgagg gcgctcgaaa
      541 gcgagatcgg tccgtcgccg tcctggcgat cgatcccacc tcgcccttca cgggcggggc
      601 cctgctcggc gaccgcctcc gcatgcagga gcacgccacc gaccccgggg tgttcatccg
      661 gtccatggct acccgcgggc acctgggcgg gctcgcgctc gcggcgccgg aggccgtccg
      721 ggtgctcgat gcctcgggcg tggacctcgt cgtgatcgag accgtgggcg ttgggcaggc
      781 cgaggtcgag gtcgccaccg aggcggacac gacgctcgtg gtggtctcgc ccggatgggg
      841 agacgcggtg caggcgtcga aggcgggcat cctggagatc gccgacgtct tcgtggtgaa
      901 caaggccgac cgcgagggcg ctcgcgagac cgcccgcgac ctggagcaga tgctccacct
      961 cggtcccgag ctcccgtggc cgcccccggt cgtcctgacc tcgacggtga acggcgaggg
     1021 catggaggag ctttgggagg cgatcgaggg gcaccagaag cacctggagt ccaccggcga
     1081 gctggaccgc cgtcggcggg ccaggctcct ccgggaggtc gagggcatcg tggcggaacg
     1141 cctccgggtc cgggcgcagt ccctcttgga gcggggtgac ctggaggccc tggccgacga
     1201 cctcgcggcc cgacgcaccg acccgtacgc cgcggccgat atcctggtgg gcaggctcga
     1261 cgaggaggct cgatgaacaa ggaggagtgg cgccagcggt accgccaggc gggggagcgc
     1321 gatgccgact tcgagacgat cagcggcgag cccatcgagc cgctgtacac cccggaagac
     1381 ctcgagggct tcgatcccga ccgggacctc ggcttccccg gcgagtatcc gtacacccgc
     1441 ggcgtctatc cctccatgta tcgaggccgg ctgtggacga tgcgccagtt cgccggcttc
     1501 ggcagcccca agcagacgaa cgagcgttac aagtacctcc tgaagcacgg ccagggcggt
     1561 ctctcggtcg ccttcgacat gcccacgctg atgggccggg actccgacga cccgcacgcc
     1621 gagggcgagg tgggccgctg cggcgtcgcg accgactcgc tggccgactt cgagatcctc
     1681 ttcgacggca tcccgctcga tgacatcacg acctcgatga cgatcagcgg gccggccgcg
     1741 atcctcttcg ccttcttcct ggccgccgcg gagaagcagg gggtggactg gacgacgctc
     1801 gacgggaccc tccagaccga catcctcaag gagtacatcg cgcagaagga gtggatcttc
     1861 cctccccggc cgcacctgcg gatcatcggc gacctcatgg agttctgtgc ggacgaggtg
     1921 ccgcagtggc acccgatcag cgtctccggc tatcacatcc gagaggccgg ctcgaccgcg
     1981 gtccaggagc tggcgttcac gctggccgac ggcttctcgt atgtggagct cggcaaggag
     2041 cggggcctcg acgtggatcg cttttcgcag gggctgtcat tcttcttcaa cgcgcacatc
     2101 gacttcttcg aagagatcgc caagttccgc gccgcgaggc gcatctgggc ccgctggatg
     2161 aaggagcgct acggcgcgga ggacccgcag tcgtggcgcc ttcggttcca cacgcagacc
     2221 gccggctgtt cgctgaccgc tcagcagccc gagaacaaca tcgtccggac ggcgatcgag
     2281 gcgctggccg gcgtgctcgg cgggacgcag tcgctccaca cgaacgcgct ggacgaggtg
     2341 ctggcgctgc ccaccgagaa ggcggcgcag atcgccctgc ggacccagca ggtgatcgcc
     2401 cacgagaccg gcgtggcgaa cgtgatcgac ccgctggggg gctcgtactt cgtggaggcg
     2461 ctgacgaaca aggccgagga gcaggccgag gagtacttcc gcaagatcga ggagatgggc
     2521 tcgggctcga tcctcgaggg gatgctcgtg ggtatcgagc ggggctactt ccagtccgag
     2581 atcgccgacg ccgcgttccg cgagcaggag cggtacgaga aggg
//
LOCUS       DASUTW010000176         2112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_55519, whole genome shotgun sequence.
ACCESSION   DASUTW010000176 DASUTW010000000
VERSION     DASUTW010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55519"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2112)
                     /locus_tag="VF058_06030"
     CDS             complement(<1..>2112)
                     /locus_tag="VF058_06030"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004945256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX6207903.1"
                     /translation="LEHGAIPEGLLDEVKSHLRSLESDMDKKLGDLEDPLLVSVRSGA
                     KYSMPGMMDTVLNLGLNDRSVLGLARQARSDRFAFDSYRRFIQMFGKIVMDIPGDLFE
                     EALDEAKERKGEGALDTDLEAIDLRDLVERFKVIFKEQTGRLFPEDPQEQLSMAIEAV
                     FRSWNNPRAHVYRRQERIPDDLGTAVNVQAMVFGNLGDDSGTGVAFTRNPATGEPGVY
                     GDYLLNAQGEDVVAGIRHTLPLGDLERLNAKAYRELLQIMETLERHYRDMCDIEFTIE
                     RGKLWMLQTRVGKRTGGAAVRMAVDMANEGLISREEAVGRVEPEQLDQLLHPQFDPGA
                     DIEVLAKGLNASPGAAVGRAVFDADTALEWGERGEDVILVRRETNPDDLHGMIAARGI
                     LTSHGGKTSHAAVVARGMGKPAVCGAEALKIDLGGRRFEVDGAAVKQGDVISIDGTTG
                     RVVVGEVPLREPKLSGDFETLLGWADDLRQLGVRANADDGPGAEKAREFGAEGIGLCR
                     TEHMFGTPGDERLPIFRQVILSAPAARAAQLALEEAEREAGAARGSAKKPARERVKQA
                     KARMSSEEVKPYFDGLRKLQAGQRKDFEAVLKAMDGLPVTIRLLDPPLHEFLPSSHEL
                     EMALAFPTRKANADLIREFGGKRKAETVLREVLGLEENNPMLGLRGCRLGILYPDIYR
                     AQVRAILEAAVNRKKRGGDPKV"
BASE COUNT          270 a          766 c          713 g          363 t
ORIGIN      
        1 accttgggat ccccgccccg cttcttgcgg ttgacggccg cctccaggat ggcgcggacc
       61 tgcgcccggt agatgtccgg gtagaggatc cccaggcggc atcccctcag gcccagcatc
      121 gggttgttct cctcgagccc cagcacctcg cggaggacgg tctccgcctt ccgcttcccc
      181 ccgaactccc ggatcaggtc cgcgttcgcc ttgcgggtcg ggaacgccag cgccatctcc
      241 agctcgtgcg aggatggaag gaactcgtgg agcggcgggt cgaggagtcg gatcgtgacg
      301 gggagcccgt ccatcgcctt gagcacggcc tcgaagtcct tgcgctggcc ggcctggagc
      361 ttccggagcc cgtcgaagta gggcttcacc tcctcggagc tcatcctcgc cttggcctgt
      421 ttgacgcgct cccgggcggg cttcttagcc gagcctctgg cggccccggc ctctcgctcg
      481 gcctcctcca gcgcgagctg ggcggcgcgt gccgccgggg ccgagaggat cacctgccgg
      541 aagatgggga gccgctcgtc gccgggggtt ccgaacatgt gctccgtgcg acacaggccg
      601 atcccctcgg ctccgaactc cctcgccttc tcggcccctg ggccgtcgtc ggcgttggcc
      661 ctgaccccca gctggcggag gtcgtcggcc cagccgagca gggtctcgaa gtcccccgag
      721 agcttgggct cgcgcagggg gacctccccc acgacgacgc gcccggtggt cccgtcgatc
      781 gagataacgt caccctgctt gacagccgct ccgtcaacct cgaatcgtct gccccccaga
      841 tcgatcttca gggcctccgc cccgcagacc gcgggcttgc ccatcccgcg ggccacgacc
      901 gcggcgtggg aggtcttgcc accgtgggag gtgaggatgc cgcgggcggc gatcatgccg
      961 tggaggtcgt cggggttcgt ctcccgccgc acgaggatca cgtcctcgcc tctctccccc
     1021 cactccaggg cggtgtcggc atcgaagacg gccctcccga ccgccgcgcc ggggctggcg
     1081 ttcagcccct tcgccagcac ctcgatgtcc gctccggggt cgaactgggg gtgcaggagc
     1141 tggtcgagct gctcgggctc caccctcccc acggcctcct cgcgcgagat gaggccctcg
     1201 ttcgccatgt ccacggccat gcggacggcc gcgcccccgg tgcgcttgcc cacccgggtc
     1261 tggagcatcc acagcttgcc gcgctcgatg gtgaactcga tgtcgcacat gtcccggtag
     1321 tggcgctcga gggtctccat gatctggagc agctcgcggt aggccttcgc gttcagccgc
     1381 tccaggtctc ccagcggcag cgtgtggcgg atcccggcca cgacgtcctc cccctgcgcg
     1441 ttgaggaggt agtccccgta cacgcccggc tccccggtcg cggggttccg cgtgaacgcc
     1501 acgccggtcc cggagtcgtc cccgaggttc ccgaagacca tggcctgcac gttcaccgcg
     1561 gtccccaggt cgtcggggat gcgctcctgg cggcggtaga cgtgggcccg ggggttgttc
     1621 caggagcgga agacggcctc gatggccatg gagagctgct cctgggggtc ctcggggaac
     1681 agcctgcccg tctgctcctt gaagataacc ttgaagcgct ccacgaggtc gcggaggtcg
     1741 atcgcctcga ggtccgtgtc cagcgcgccc tcgcccttgc gctccttggc ctcgtcgagc
     1801 gcctcctcga agaggtcgcc ggggatgtcc atcacgatct tgccgaacat ctggatgaag
     1861 cggcggtagg agtcgaaggc gaatcggtcg ctccgggcct gtcgggccag gccgagcacc
     1921 gaccggtcgt tcaggcccag gttgaggacc gtgtccatca tgcccggcat ggagtacttc
     1981 gcgcccgagc gaacggagac caggagcggg tcctcgaggt cgccgagctt cttgtccatg
     2041 tcgctctcga ggctgcgcag gtggctcttg acctcgtcga ggaggccctc ggggatggcc
     2101 ccgtgctcca gg
//
LOCUS       DASUTW010000177        19475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1739742, whole genome shotgun sequence.
ACCESSION   DASUTW010000177 DASUTW010000000
VERSION     DASUTW010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 19475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19475
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1739742"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VF058_06035"
     CDS             complement(<1..324)
                     /locus_tag="VF058_06035"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HEX6207904.1"
                     /translation="MADEEPLVGILVGSASDLPTIQQAGAILERFEIPHEIEVMSAHR
                     SPDAVDEYARTAEERGLVTIICGAGMAAHLAGAVAARTSLPVIGVPIGGGSLGGEDAL
                     LSTVQM"
     gene            complement(314..1582)
                     /gene="purD"
                     /locus_tag="VF058_06040"
     CDS             complement(314..1582)
                     /gene="purD"
                     /locus_tag="VF058_06040"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX6207905.1"
                     /translation="MKVLVVGSGGREHALAWAIARSPGAEVHAAPGNAGIEEVARRHA
                     VDAESVDAIVALVEREGIELTVVGPEAPLVVGLADRLRERGHRAFGPGAEGARLEGSK
                     SWAKDLCRRHGIPTPRAEVFEDLDEAAAFLDSMEPPYVVKADGLAAGKGVAVCATRED
                     AVQALKERLVERAFGEAGATVLVEEHAAGREVSALALTDGETIVPLVLAKDFKRALDG
                     DEGPNTGGMGAYSPVSFVGEALEAEIRALLTRTMGALRDEGIEYRGVIYAGLMLTDQG
                     PRVLEFNCRFGDPETQAVVPRIGSDLLEALAACAEGRLAEVELTARPDACVTVVAASG
                     GYPGSYEMGKEIRGVDEAASREGVVVFHAGTEQTDGRLVAAGGRVLAVSALGESVAAA
                     RRHAYDALSLISFDGMHYRTDIAKEAADGR"
     gene            complement(1579..2856)
                     /locus_tag="VF058_06045"
     CDS             complement(1579..2856)
                     /locus_tag="VF058_06045"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017544402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX6207906.1"
                     /translation="MPATILVGTQWGDEGKGKATDLLADRMDLVVRYQGGNNAGHTVV
                     VGDRTLKLHLIPSGILYPHITSVIAAGVVVDPKVLLEEMDELAAQGIDTSRLVLSANA
                     HLIMPYHLELDRVTERYLGRSRLGTTKRGIGPAYADKAARIGLRVQDLTDEKIFRKKL
                     EVVLKEKNAILSKVYNRLPLEAEEIVRDYMAYGERLAPHIADTSAVLHEALREGRSLL
                     LEGAQGTLLDLDHGTYPFVTSSNPVAGGALAGAGLGPGVVDRVIGVTKAYVTRVGSGP
                     FPTEDEGAAGRHMAERGVEVGTTTGRARRCGWLDVVLLRYAARVNGLTELFLTKIDVL
                     TGLSRLKVCTAYRYEGETFEDFPPHQTIVHKAEPVYEELDGWEEDLVDARSLDDLPGA
                     ARKYVALIEEASGVPVRTISVGPGREDTVEVAA"
     gene            complement(2933..3136)
                     /locus_tag="VF058_06050"
     CDS             complement(2933..3136)
                     /locus_tag="VF058_06050"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX6207907.1"
                     /translation="MAVVKTIELVGVSSESWRDAAAQALAEATKTLRGIEGMDVLETS
                     AVVDGSDISEYHTRVRIKFRIER"
     gene            complement(3225..3899)
                     /locus_tag="VF058_06055"
     CDS             complement(3225..3899)
                     /locus_tag="VF058_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207908.1"
                     /translation="MQDLESELRLLMRDRAERLTSPPPKRQRPGASKAPRRRIVVMAV
                     GALLAVAGAGIAISQGVGPITIVPRSESFEVASGETEEGSWRLIFYLAEIETGSGEPP
                     EKAWCLDLDRSWAQEPGARGPQEANVCTEGGDQATGVGPVARFPRFAGDDALVYGAVS
                     PDIASLEVHDTKGRTRETVEIIPAPEESGTRMGLFATFVEDLGVRRVDVVAFDRDGRI
                     SEVERV"
     gene            complement(3902..4435)
                     /locus_tag="VF058_06060"
     CDS             complement(3902..4435)
                     /locus_tag="VF058_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6207909.1"
                     /translation="MERTTAHLGDQTRDQGSLLEDLYADHALRAFRLAYLLTGSREAA
                     EDVVQEAFLQVGDRLDSLRDLRAFPGYLRTVVLNLVRGRSRRMRRERLLTERAAILEP
                     RTRREEVDIGQRERLWAALHLLPHRQRAALVLRYYEDLSERDAAMVMGTTVSAVKALV
                     SRGTRALRAMLGDMREA"
     gene            4590..6134
                     /locus_tag="VF058_06065"
     CDS             4590..6134
                     /locus_tag="VF058_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme peroxidase family protein"
                     /protein_id="HEX6207910.1"
                     /translation="MDRHGSESYFVLGEGLLAESVGGRERTLATAGGAPPFRFSRMGP
                     RGSGRQLSEANRKRIAAAMVGGDRRSSNIPAGFTYLGQFVDHDLTFDKTEVMLGENVS
                     PADLLQARSPSLDLDSLYGAGPTDPASARFYEDDGIHLKMGRTTPESGVGAKDGFDLP
                     RGEGSTAKAKRRAVIPDPRNDENLAVAQTHLAFIRFHNRVVDSLPASVPTSQRFARAR
                     RIVTRHYQWMLRTDYLPRICAKGVVNSVFNNGRKAFEVGVAPTAVPTMPIEFSVAAFR
                     LGHSMIRDDYNWNRFFDFGSGTLDLLFEFSATSGTLGGSQRLPSNWIADFRRLYDFGE
                     AGRDNLTVPEGRFNRAMRIDTSLAATLRRLPRLPRAESNLALRNLTRGTMVTLATGQQ
                     MARFLRSKGVAVTTLSKQRIRDGRRGADLDGLTGPQRDALLKNTPLWFYVLREAEFNG
                     GRLAGVGARIVAETFHRAMEGSQTSIVRDPAWRPSLGPNRTTFRMVDLLLFAFEGKKS
                     LLAPLG"
     gene            complement(6148..7050)
                     /locus_tag="VF058_06070"
     CDS             complement(6148..7050)
                     /locus_tag="VF058_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2090 domain-containing protein"
                     /protein_id="HEX6207911.1"
                     /translation="MALGFDGSLYILAFDHRGSFRKKMGVGSDPSSEEVQKLRDGKSL
                     IFEGFQQAISEGAPRDAGGVLVDEEYGTDVARKAKADGYRFAMPVEKSGQDEFDFEYG
                     EEYGKHIEDFDPTFSKVLVRYNPEGDREMNARQLVRLKELGDWLHERDRLYLFELLVP
                     ATEEQLASVDGDADRYDREVRPRLMLDAIRDLHGGGVEPDIWKIEGLDKREDCEAVSE
                     LVRSDGRDGVACIVLGRGADDAAVDHWLRQGSGVPGYRGFAIGRTIWGPPVMDFVEGK
                     VDRSEAASRIGQSYRRFIDVYEGA"
     gene            complement(7070..7303)
                     /locus_tag="VF058_06075"
     CDS             complement(7070..7303)
                     /locus_tag="VF058_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207912.1"
                     /translation="MGAVVGLVVAAVAIGAGNDGCGASAREECFELARMLAIRTGLVA
                     GIATMVMLLLVAGLLKMVAHDEARRTEPASPWD"
     gene            complement(7359..8006)
                     /locus_tag="VF058_06080"
     CDS             complement(7359..8006)
                     /locus_tag="VF058_06080"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6207913.1"
                     /translation="MLDRFTWYKQSSYRWKGDDLVMYIDPWQITGDPEPADVIFLTHA
                     HYDHYSPDDLGRLRKEDTQIVAPHDIAAELTGNVQAVAPGDAVEAGGIKAQAVPAYNT
                     VEERLKSHPKSSNWVGYILELGGVSHYFSGDTDHLPELESVSARAAFVCVGGEIFTMG
                     PQEAADLVKAIGPEVAVPNHYAFECGTRSDAEAFKEAAAPIKVEIMSPEHDFDLE"
     gene            8085..9446
                     /locus_tag="VF058_06085"
     CDS             8085..9446
                     /locus_tag="VF058_06085"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6207914.1"
                     /translation="MATSAADARARVGLRDTLSLLGRNRAFARLYGAQLISFMGDWFT
                     TVALLGLVSDLTGSPVLVSLVLVAHTIPFAILAPLGGHLADRFDRQKLMIGADIGRAV
                     LAPLYLLVDSPGEVWLAFAVMASVGGIGAVFNPAAQAAVPNLVDPEDLPAANVLVGSA
                     WGAMLAVGAALGGVVAATLGRDAAFLGDGLTFVLSALLLIGIKRRFSEAREEHEERRG
                     IARSMGESFRYARGDSRVLALLAVKAGFGLSTGVLAILPLFALDVWDAGDQGTGILFA
                     ARGAGAVIGPFLVRRFIGSLRGLFVAIGLSFAVYGVGYGVFPFSTGIAVATLPVFLAH
                     LGGGAQWTLSTYGLQRIVPDRFRGRLFAIDYGLVTLTIAGSLMAAGWAAEHVSPTAVM
                     LTLAAVSLGWAVAWTSLTSKLRREGSFDATPGPEAPVPEAAGAPHPEEETQPGAPPPL
                     ASP"
     gene            complement(9346..10008)
                     /locus_tag="VF058_06090"
     CDS             complement(9346..10008)
                     /locus_tag="VF058_06090"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX6207915.1"
                     /translation="MIVLWVVLGIVALLVIGGIVSYNRFVQQRQAIKDAFANIDTELR
                     RRYDLIPNLVETVRGYQIHEKEVLEEVTRARSAATAATGSPAEQAAAEGPLVAALRQL
                     FAVVENYPQLRANENFLALQQELAVTEDRLQTARRFYNANVRDYNQRVQGFPSNIIAS
                     WFNFEEEEFFEIEEAMRETMEQAPQVDFTGTRGEAEPPAGSPPPGGEPPPPPAQEPPG
                     QA"
     gene            complement(10005..10709)
                     /locus_tag="VF058_06095"
     CDS             complement(10005..10709)
                     /locus_tag="VF058_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207916.1"
                     /translation="MDPVLMFILGLVGVAVVGGLSWYFSHKRREAFALFARSHGMTYS
                     RHDPYGTINLPFRLFTKGDGRKVENVLSGDWRGSPVRAFDYWYYTESSDSKGNRTRTY
                     YRHTCALLEIPAAFSPLAITRENVFTRMADGLGFRDIEFESPEFNKRFQVKAKDRHFA
                     YAFIDGRMMKWLLGLPRKWEFEIVGAWILAYERKRMKPEEFPPLIEMALAFRQQVPRA
                     ALSLYGRDVETREERA"
     gene            10949..11512
                     /locus_tag="VF058_06100"
     CDS             10949..11512
                     /locus_tag="VF058_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207917.1"
                     /translation="MGALALGLLVAAASCGGDGYEVSELPAIVLQASEAPEGTVHERD
                     ESGTRALREFSVSGQERLQGEGFVDGYATVFGPTGGVTTHLEVTSGAMVFEDGSGAEA
                     SMAVVLQDVEGRLIEATEMSADGLGAESAGLSGPVEGDARQRSALLYVWRTDNLVLFV
                     IASALLESAPSPEVILGLAETMDSRAS"
     gene            complement(11595..11957)
                     /locus_tag="VF058_06105"
     CDS             complement(11595..11957)
                     /locus_tag="VF058_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207918.1"
                     /translation="MHEIGLMYRWGKLVPGRERAGIEHFASAIEFFGKKLAEGVITFF
                     EPYFSRTTDLEAEAGFIVIKGPREKVLELMEDEEYLVLLTKTSYLVEHFTVEMLTVGE
                     EIQVQLERAQKVFAELGI"
     gene            12336..12596
                     /locus_tag="VF058_06110"
     CDS             12336..12596
                     /locus_tag="VF058_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207919.1"
                     /translation="MTSVDLEGDIDQAIALIRDRIVPQASRLEGIVAAYWLGNRNTRR
                     GLAVTVWESEEAMLARTSHGEQRGENGRRRKWTRLQWSTTRL"
     gene            complement(12708..14072)
                     /locus_tag="VF058_06115"
     CDS             complement(12708..14072)
                     /locus_tag="VF058_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX6207920.1"
                     /translation="MRTRRFAPMLGALLAISLSVLPAAPSGAQSPMGDPMDVGLELIA
                     EGLTSPVALMEAPDGSGRLFVVDQAGLIRIITSEGELLAEPFLDVRDRMVALQAGFDE
                     RGLLGLAFHPEYASNGRFFVYYSAPLREGAPAGFNHTSHISEFTVSDDADRADPSSER
                     ILLQVDQPQFNHNAGALAFGPHGYLFISLGDGGGANDVGLGHVEDWYADNAGGNGQDV
                     EQNLLGNILRIDVDGGDPYGIPADNPFVGTVGLDEIWAYGLRNPYRMSFDMATGDLFV
                     SDAGQGLWEEVNLVTRGANLGWNVKEGTHCFDAENNTESPAECPDEVLSGPRAGDPLV
                     DPVIEYANGRQPGGIGLVVVGGFVYRSSTLPQLQGRYVFGDWSTSFGNPDGTLLVAKS
                     RKKGLWQIQELRVATSPTGRLDHFLLGFGQDSAGDVYVLGTERTGPTGATGKVWRLVR
                     PSGR"
     gene            14318..15868
                     /locus_tag="VF058_06120"
     CDS             14318..15868
                     /locus_tag="VF058_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207921.1"
                     /translation="MQLVSRLDTSGLRLQGWQAKRWKGAFGMRSRAASSILVALALAI
                     APVPGASAEPPAVSRGLEFVGATDLGLPFPTMNTEIEIRGDHVYVGSFFPLDQNGQTA
                     FKVADVSDPANPALATHFMLPGTVLDTQVNNDVARCGYGKNLAGLNDPACNTVLATTQ
                     SAPGDVDGLWLFDVRDPANAQPWGWDSTKSFVDQVGFIQRPARGSHNGFLFGDFAFAA
                     GIGEASFEIWDISPLFSDPAAAPTLAATYSNLADPRPVRPQREREGSHDIFVQRNPDG
                     RIFAYVAGPNFYIVDVTDVVEGATRGDISDHLVGFNNYVNADGTLTVTRPHGGHYGEP
                     TDDGDHAYVGDETGCGEPGIIHILDTSGLPQVGGTPKQLAEVGTVTWPTSNVPQCANR
                     NADDSADDPQNRNGDTQNFCRTGHNFRIYGDIMTHGNYCEGVFVWDLTDRANPTLLAQ
                     HRSVGDYGGKRKELKDHAAKADLHSTFWQAVSDGDPDALVRGEQFIYGSDLSAGLVVL
                     RMRPAGSG"
     gene            15978..16313
                     /locus_tag="VF058_06125"
     CDS             15978..16313
                     /locus_tag="VF058_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207922.1"
                     /translation="MANDVERPGVESSSSLSLTGSPLGGITAEDAGTGFWFVRFPKKV
                     TGKSIFVSLQGKNISHGSINAVPCGGGVGNARATSCSANDNGNHVLVGTYDENGDPVD
                     RPFYIMAVG"
     gene            complement(16399..16983)
                     /locus_tag="VF058_06130"
     CDS             complement(16399..16983)
                     /locus_tag="VF058_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX6207923.1"
                     /translation="MAGLGTLLLTGVVASGATVPGWERAVFHAINGLPSWLRVPMQAA
                     EILGVLLVGPAVAVIALGFRKPRLAAAALLATVLKLALERVVKLVVERARPAVSTPDA
                     IARGVPIRGLAFVSGHAVLTAALAGVVTPYLRGAWKALPWALVGLVCFARVYLGAHNP
                     LDVVGGAGLGLAIAGALNLILGVPAWGIEEEGAR"
     gene            complement(17079..>19475)
                     /gene="clpB"
                     /locus_tag="VF058_06135"
     CDS             complement(17079..>19475)
                     /gene="clpB"
                     /locus_tag="VF058_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX6207924.1"
                     /translation="GRVEERLDQIPKVYGTTQEARLAPATARVLEGAADEAEALTDEY
                     VSTEHLLLAMLADQGPLGSLLRELGLDRDRLLAALAEVRGRQRVTDENPEEKYEALER
                     YGRDLTDLARRGKLDPVIGRDDEVRRVIQVLSRRTKNNPVLIGEPGVGKTAIAEGLAQ
                     RIVAGDVPESLKGRRIVALDIGAMVAGAKYRGEFEDRLKAVLQEITASERQIITFIDE
                     LHTIVGAGAAEGAVDAGNMLKPLLARGELRMIGATTLDEYRKHVEKDAALERRFQPVF
                     VGEPSVEDTIAILRGLRERYEVHHGVEIKDEALVAAAMLSDRYVTGRFLPDKAIDLVD
                     EAASRLRIEIDSMPVEIDEVDRRVKQLEIERAALKKETDEASRQRLEKIEQELADLNE
                     QLSAMRAHWDREKEHISRIHELKSELEETRGQAERAERDGDLERAAELRYGRIVELRK
                     ELEAENAALAEIQSDQKMLKERVDEEDVAEVVSAWTGVPVSRLLEGEMQKLVRMEEAL
                     HERVVGQDEAVTAVANAIRRSRAGLSDPNRPIGSFIFLGPTGVGKTELARALAEFLFD
                     DERAMVRIDMSEYQERHAVARLIGAPPGYVGYEEGGQLTEAVRRRPYSVILLDEIEKA
                     HADAFNLLLQVLDDGRLTDGQGRTVDFKNAVVIMTSNLGSRWFFEETLSPEDRRERVL
                     AEMREAFRPEFLNRVDDVIVFEPLDKEQIAEIVDIQVRRFAERLAERRLTLKLTGDAR
                     SFLAERGWDPALGARPLKRLIQKEIQDKLALALLEGEITDGDHVVADRQGDGLIVRRG
                     "
BASE COUNT         2942 a         6969 c         6453 g         3111 t
ORIGIN      
        1 catctggacg gtggagagga gggcgtcctc gccgcccagc gaccccccgc cgatcgggac
       61 gccgatcacc ggcagggagg tccgtgcggc caccgcgccc gccaggtgcg cggccatgcc
      121 cgccccgcag atgatcgtga cgaggccccg ctcctccgcg gtgcgcgcgt actcgtccac
      181 ggcgtcgggg ctccggtgcg cggacatgac ctcgatctcg tgggggatct cgaagcgctc
      241 caggatcgcc ccggcctgct ggatcgtggg gagatccgag gcggagccca cgaggatccc
      301 caccagcggc tcttcatcgg ccatcagctg cctccttcgc gatgtcggtc cggtagtgca
      361 tcccatcgaa cgagatcagc gagagcgcgt cgtaggcgtg gcggcgggcg gccgcgacgc
      421 tctcgccgag cgcgctgacc gccaggacgc ggccgcccgc cgccacgagc cgcccgtccg
      481 tctgctcggt gcccgcgtgg aacacgacca caccttcgcg cgacgcggcc tcgtccacgc
      541 cgcggatctc cttccccatc tcgtacgagc cggggtagcc gcccgaggcg gccacgaccg
      601 tgacgcacgc atcgggccgg gcggtcagct cgacctcggc caaacgaccc tcggcgcacg
      661 cggccagcgc ctccagaaga tccgacccga tccgcggcac gacggcctgg gtctccgggt
      721 ccccgaagcg gcagttgaac tcgaggaccc tcggcccctg gtcggtgagc atcaggcccg
      781 cgtagatcac gccgcggtac tcgatccctt catcccgcag ggcgcccatc gtccgggtga
      841 gcagggcccg gatctcggcc tccagcgcct cccccacgaa cgacaccggc gagtacgccc
      901 ccatgccacc ggtgttcggg ccctcgtccc cgtccagcgc gcgcttgaag tccttggcca
      961 gcacgagcgg cacgatcgtc tcgccgtcgg tgagggccag cgcggacacc tctcggccgg
     1021 cggcgtgctc ctcgaccagg acggtggctc ccgcctcccc gaacgctcgc tccacgagcc
     1081 gctccttcag cgcctgcacc gcgtcctcgc gggtggcgca gacggcgacg cccttgccgg
     1141 cggccagccc gtcggccttg accacgtacg gcggctccat agagtccagg aacgccgcgg
     1201 cctcgtcgag gtcctcgaag acctcggccc gcggggtggg gatcccgtgc cgacggcaca
     1261 ggtccttggc ccaggacttc gagccttcga gccgcgcccc ctccgcgccc ggcccgaacg
     1321 cgcggtgccc tcgctcgcgg agccgatccg cgagccccac gacgagcggc gcttcggggc
     1381 cgaccaccgt gagctcgatc ccctcgcgct ccaccagcgc cacgatcgca tcgacgctct
     1441 cggcgtccac tgcgtggcgc cgggcaacct cctcgatccc cgcgttgccc ggcgccgcat
     1501 gcacctccgc gcccgggctc cgcgcgatgg cccaggccag cgcgtgctcc cggccgccgg
     1561 accccaccac cagcaccttc acgccgcgac ctccaccgtg tcctcgcgcc ccggtcccac
     1621 cgagatcgtg cgcaccggca cgcccgaagc ctcctcgatc agcgcgacgt acttccgcgc
     1681 cgcgcccggg aggtcgtcga gcgagcgagc gtccacgaga tcctcctccc acccgtcgag
     1741 ctcctcgtac accggctcgg ccttgtggac gatcgtctgg tggggcggga agtcctcgaa
     1801 ggtctccccc tcgtagcggt acgccgtgca caccttcagc cgggacaggc ccgtcaggac
     1861 gtcgatcttc gtgaggaaca gctcggtcag cccgttgacg cgcgcggcgt agcggagcag
     1921 gacgacgtcg agccagccgc agcgtcgggc gcggccggtc gtcgtgccca cctcgacccc
     1981 gcgctcggcc atgtgacgac cggccgcgcc ctcgtcctcc gtcgggaacg ggcccgatcc
     2041 cactctggtc acgtacgcct tcgtcacccc gatcacgcgg tccaccacac ccggcccgag
     2101 ccccgcgccc gcgagcgccc cgcccgccac cgggttcgac gacgtcacga aggggtacgt
     2161 cccgtggtcc aggtcgagca gggttccctg cgccccctcc agcagcaggc tgcgaccttc
     2221 ccgcagagcc tcgtgcagca ccgccgacgt gtccgcgatg tgcggcgcca gccgctcgcc
     2281 gtaggccatg tagtcgcgga cgatctcctc ggcctccagc ggcagccggt tgtagacctt
     2341 gctgaggatc gcgttcttct ccttcagcac aacctccagc ttcttccgga agatcttctc
     2401 gtcggtgagg tcctggaccc ggagcccgat cctcgccgcc ttgtcagcgt aggccggtcc
     2461 gatcccgcgc ttcgtcgtcc ccagcctcga tctccccagg taccgctcgg tgacccggtc
     2521 cagctccagg tggtacggca tgatcaggtg cgcgttcgcc gagagcacga ggcgagaggt
     2581 atcgatcccc tgcgcggcca gctcgtccat ctcctcgagc agcaccttcg ggtccaccac
     2641 cacgcccgcc gcgatcaccg aggtgatgtg gggatagagg atccccgacg ggatcaggtg
     2701 aagcttcagc gtgcggtcac cgacgacgac cgtgtggccc gcgttgttgc ctccctggta
     2761 ccgaacgacg aggtccatcc gatcggcgag gaggtcggtg gcctttccct tcccctcatc
     2821 gccccactgg gtccccacca ggatcgtcgc aggcatcagt tcgtcggcct cggccgtagt
     2881 ctagggcacg cgagagcccc cgggcctggc ccgggggctc ggtgccctgc gatcagcgct
     2941 cgatgcggaa cttgatccgc acccgcgtgt ggtactcgga gatgtccgag ccgtccacga
     3001 ccgccgacgt ctcgagcacg tccatcccct cgatgccccg aagggtcttc gtggcctctg
     3061 cgagcgcttg agccgccgcg tcacgccacg actccgatga gaccccgacc agctcgatcg
     3121 tcttgactac cgccacggcg tcctccttga gaagcgtcgc ccgcatcgta ggacgaagat
     3181 gatgccgcgg acaagctaaa tcgtctgccc gggctccgaa ccgatcagac gcgctcgacc
     3241 tccgagatgc gaccgtctcg gtcgaaggcc accacgtcga ctcgccgcac cccgaggtcc
     3301 tccacgaacg tcgcgaagag ccccatccgc gtacccgact cctccggcgc agggatgatc
     3361 tctaccgtct cccgagtcct gcccttggtg tcatggacct caagacttgc gatatcgggg
     3421 gagacggctc catagaccag cgcgtcatcc ccggcgaagc gtgggaagcg agcaacggga
     3481 cccacgccgg ttgcctggtc gcctccctca gtacagacgt tcgcctcctg cggcccgcga
     3541 gccccgggct cctgggccca cgagcgatcg aggtccaagc accacgcctt ttcgggaggc
     3601 tccccagaac ccgtctcgat ctccgcgaga tagaagatca gccgccacga gccttcctct
     3661 gtttcgccgg aggcaacctc gaacgactca cttctcggga cgatggtgat cggtccaact
     3721 ccctgcgaga tggcgatccc cgctcccgcc acagcaagga gggcacctac cgccatcacc
     3781 actatccggc gcctcggtgc cttggatgca ccaggtcgct gccgtttggg cggtggactc
     3841 gtcaggcgct cggcccgatc cctcatgaga agccgaagct ccgactcgag gtcctgcatc
     3901 ttcaagcctc cctcatgtcc ccaagcatcg cgcgaagcgc tctcgtgcct cgcgagacga
     3961 gcgccttgac tgcactcacc gtcgttccca tcaccatcgc agcgtcgcgt tccgagaggt
     4021 cttcgtagta gcgcagcacg agcgccgccc gctgccggtg cgggagaagg tgcagggccg
     4081 cccagagtcg ctctcgctga ccgatgtcga cctcctctct ccgcgtccgc ggctcgagga
     4141 tcgctgcacg ctccgtgagg agccgttccc ggcgcatccg acgcgaacgc cccctcacga
     4201 ggttcagtac gaccgttcgc aggtagccgg ggaacgcgcg aagatctcgc aaggagtcga
     4261 ggcgatctcc gacctggagg aacgcctctt ggaccacgtc ctctgcagcc tctcggctcc
     4321 cggtcagcag gtacgcaagg cgaaacgcac gcaaggcatg atcggcgtaa agatcttcta
     4381 ggagggatcc ctgatccctc gtctggtcgc cgagatgggc cgtcgtccgc tccaccgtcc
     4441 tacctgctag atgctcgggc gagcggacag gttgcgcaac acccttccac gtggaggcgg
     4501 ctctggccgc cccctcgtaa agaatcgacc ttgacccctc cccgggacga gagaacaatc
     4561 gaccgaccat ccggcgaaag ggggccgccg tggacaggca cggatcggag agctacttcg
     4621 tcctcggtga gggcctcctc gccgagagcg tcggaggccg ggagcggacc ctcgccacgg
     4681 ccggcggcgc gccgccgttc cgcttctctc ggatgggccc gcgtgggagc ggccggcagc
     4741 tcagcgaggc caaccggaag aggatcgccg cggccatggt cggaggcgac cgcagatcgt
     4801 ccaacatccc cgccggcttc acgtatctcg gccagttcgt cgaccacgac ctgacgttcg
     4861 acaagaccga ggtgatgctg ggcgagaacg tctcgccggc cgacctgctg caggctcgct
     4921 cccccagcct cgacctcgac tccctgtacg gggccggccc cacggaccct gcctccgcga
     4981 ggttctacga ggacgacggc atccacctga agatggggag gacgacgccc gagagcgggg
     5041 tcggggccaa ggacgggttc gaccttccac gcggcgaggg atcgacggcg aaggccaagc
     5101 gccgcgccgt gatccccgac ccgcggaacg acgagaacct cgctgtggcc cagacccacc
     5161 tcgccttcat ccgcttccac aaccgcgtcg tggactcgct cccggcctcc gttccgacct
     5221 cgcagcgttt cgcgcgcgcc cggcggatcg tcacccggca ctaccagtgg atgcttcgca
     5281 ccgactacct ccccaggatc tgcgcgaagg gcgtggtgaa cagcgtcttc aacaacggca
     5341 ggaaggcgtt cgaggtgggg gtcgctccga ccgccgtccc cacgatgccg atcgagttct
     5401 ccgtcgcggc gttccggctg gggcactcga tgatccggga cgactacaac tggaatcgct
     5461 tcttcgactt cggcagtgga accctcgacc tcctcttcga gttctccgcc acgagcggga
     5521 cgctgggagg gagccagcga ctccccagca actggatcgc cgacttccga cgcctctacg
     5581 acttcggcga ggctggacgg gacaacctca cggtccccga ggggaggttc aaccgggcca
     5641 tgcggatcga cacgtcgctg gccgcgaccc tgaggaggct cccacgcctc ccccgggcgg
     5701 aatccaacct cgctctccgg aacctcacgc gggggacgat ggtgaccctc gccacagggc
     5761 agcagatggc gaggttcttg cggagcaagg gcgtcgcggt gactacactg agcaagcaaa
     5821 ggatacggga cgggcgcagg ggcgcggacc tcgacgggct caccgggccc cagcgagacg
     5881 cgctgctcaa gaacacacct ctgtggttct acgtccttcg cgaggcggag ttcaacggag
     5941 gaaggctggc gggcgtcggc gccaggatcg tcgcggagac gttccaccgg gcgatggagg
     6001 ggagccagac gtcgatcgtc cgcgatcccg cctggcgccc ctcgctcggg ccgaacagga
     6061 cgaccttccg catggtcgat ctgctgctct tcgcgttcga ggggaagaag agcctgctcg
     6121 cgcccctcgg ctagagaccc ccttcgctca ggcgccctcg tagacgtcga tgaagcgccg
     6181 gtagctctgg ccgatccgcg aggccgcctc gctgcggtcg accttgccct ccacgaagtc
     6241 catgaccggc ggtccccaga tcgtgcggcc gatggcgaag ccacggtagc ccgggacgcc
     6301 ggacccctgg cgaagccagt ggtccaccgc cgcgtcgtcg gcgcctcgcc ccagcacgat
     6361 gcacgccacc ccgtcgcgcc catcggaccg caccagctcg ctgaccgcct cgcagtcctc
     6421 gcgcttgtcg agcccctcga tcttccagat gtcgggctcg accccgccac cgtggaggtc
     6481 acggatcgcg tccagcatca ggcgcggccg gacctccctg tcgtaccgat ccgcgtcccc
     6541 gtccaccgag gccaactgct cctcggtcgc cggcacgagc agctcgaaca gatacagccg
     6601 gtcgcgctcg tggagccagt ccccgagctc cttcagccgg acgagctggc gcgcgttcat
     6661 ctcacggtcg ccctcggggt tgtagcggac gagcaccttc gagaacgtcg ggtcgaagtc
     6721 ctcgatgtgc ttgccgtact cctcgccgta ctcgaagtcg aactcgtcct gcccgctctt
     6781 ctccacgggc atcgcgaacc ggtacccgtc ggccttcgcc ttgcgggcca catcggtccc
     6841 gtactcctcg tccacgagga cgccgcccgc atcgcgcggc gcgccctcgg agatcgcctg
     6901 ctggaacccc tcgaagatca acgacttgcc gtcgcggagc ttctgcacct cctcggaact
     6961 cggatcgctc ccgaccccca tcttcttgcg gaacgatccc ctgtggtcga acgccaggat
     7021 gtacaggctc ccatcgaagc ccagcgccat ctcccatccc tccttgtcgt cagtcccacg
     7081 gcgaggccgg ctcggtccgc cgagcctcgt cgtgcgccac catcttcagc aatccagcca
     7141 ccaggaggag catcaccatc gtggcgatgc ccgccaccag cccggtgcgg atcgcgagca
     7201 tccgggccag ctcgaagcac tcctcgcggg cgctcgcccc gcagccgtcg ttccccgcgc
     7261 cgatcgccac ggccgcgacg acgagcccga cgaccgcccc catcgcgagg acgatcccca
     7321 cgacgcgagc ccgagtgagg aggaagccct tcgtggcgct actccaggtc gaagtcgtgc
     7381 tcggggctca tgatctcgac cttgatcggg gccgctgcct ccttgaaggc ctcggcgtcc
     7441 gacctcgtgc cgcactcgaa cgcgtagtgg ttcggcacgg ccacctccgg cccgatcgcc
     7501 ttcaccaggt cggccgcctc ctggggtccc atcgtgaaga tctcaccccc cacgcacacg
     7561 aaggccgcgc gcgcgctcac gctctcgagc tcgggcaggt ggtcggtgtc gccgctgaag
     7621 tagtgggaca cgccgcccag ctccaggatg taccccaccc agttgctgct cttggggtgc
     7681 gacttcaggc gctcctcgac ggtgttgtag gcgggcacag cctgggcctt gatcccgccc
     7741 gcctcgaccg cgtcacccgg cgccaccgcc tggacgttgc cggtcagctc ggccgcgatg
     7801 tcgtgcggcg ccacgatctg cgtgtcctcc ttgcggaggc ggcccaagtc gtcgggcgag
     7861 tagtggtcgt agtgcgcgtg cgtgaggaag atgacgtcgg cgggctcggg atcccccgtg
     7921 atctgccagg gatcgatgta catcacgagg tcgtcgccct tccaccggta ggacgactgc
     7981 ttgtaccagg tgaaccgatc cagcatcgga acctcctcgg aacgctcgct ccacagccta
     8041 cccggagggc ggcgacaact accatccctg ctaccctcgc cgccatggcc acctccgcgg
     8101 cggacgcgcg cgcccgggtc gggctgcgcg acacgctgtc gctcctggga cggaaccgtg
     8161 cgttcgcgcg gctctacggc gcgcagctga tctcgttcat gggcgactgg ttcacgacgg
     8221 tcgccctgct cgggctggtc tcggacctga cggggtcgcc ggtgctcgtg tcgctcgtgc
     8281 tggtggcgca cacgatcccg ttcgcgatcc tggcgccgct cggggggcat ctggccgacc
     8341 gattcgatcg acagaagctc atgatcgggg cggacatcgg gcgggccgtg ctggccccgc
     8401 tctacctgct cgttgattcc ccgggggagg tctggctggc cttcgcggtg atggcctcgg
     8461 tcggcgggat cggggcggtg ttcaaccccg cggcccaggc ggcggtcccc aacctcgtcg
     8521 accccgagga cctccccgcg gccaacgtgc tcgtcggttc agcgtggggc gcgatgctcg
     8581 cggtgggcgc ggcgctgggc ggcgtggtgg cggccacgct cggacgcgac gccgcgttcc
     8641 tgggggacgg gctgacgttc gtgctctcgg ccctcctcct gatcgggatc aagcgccgct
     8701 tctccgaggc ccgcgaggag cacgaggagc gccgcggcat cgcccggtcg atgggcgaga
     8761 gcttccgcta cgcacgcgga gactctcgcg tgctggccct gctcgcggtg aaggcgggct
     8821 tcgggctctc caccggtgtg ctggcgatcc tgccgctgtt cgcgctcgac gtctgggacg
     8881 cgggcgacca ggggacgggg atcctcttcg ccgctcgcgg ggccggcgcc gtgatcgggc
     8941 cgttcctcgt gcggcgcttc atcggctcgc tccgggggct gttcgtcgcg atcgggctgt
     9001 cgttcgccgt gtacggggtg gggtacgggg tcttcccgtt ctcgaccggg atcgcggtgg
     9061 cgacgctgcc ggtgttcctg gcccatctcg gcggaggcgc ccagtggacg ctctccacgt
     9121 acggcctcca gcggatcgtc cccgaccggt tccggggccg gctcttcgcc atcgactacg
     9181 ggctcgtgac gctgaccatc gccggctcgc tgatggcggc gggatgggcg gcggagcacg
     9241 tctccccgac cgcggtgatg ctgaccctgg ccgccgtctc gctgggttgg gccgtggcct
     9301 ggacctccct gacgtcgaag ctgcgccgcg agggttcctt cgacgctacg cctggcccgg
     9361 aggctcctgt gccggaggcg gcgggggctc cccacccgga ggaggagacc cagccggggg
     9421 ctccgcctcc cctcgcgtcc ccgtgaagtc gacctgcggg gcctgctcca tcgtctcccg
     9481 catcgcctcc tcgatctcga agaactcctc ctcctcgaag ttgaaccagg aagcgatgat
     9541 gttcgacggg aagccctgca cccgctggtt gtagtcgcgg acgttcgcgt tgtagaagcg
     9601 gcgtgcggtc tggaggcggt cctcggtgac ggccagctcc tgctggaggg cgaggaagtt
     9661 ctcgttggcc cggagctgcg ggtagttctc gacgacggcg aagagctgcc gcagggcggc
     9721 gacgagcggt ccctcggcgg cggcctgctc ggccggcgac cccgtggccg cggtcgctgc
     9781 ggatcgggcc cgggtgacct cctcgaggac ctccttctcg tggatctggt agccgcgcac
     9841 cgtctcgacg aggttcggga tgaggtcata gcggcgccgc agctcggtgt cgatgttggc
     9901 gaacgcgtcc ttgatcgcct gacgctgctg gacgaaccgg ttgtaggaca cgatgccgcc
     9961 gatgaccagc agggcgacga tgcccaggac cacccacagg acgatcatgc gcgctcctcc
    10021 ctcgtttcga cgtcccgacc atacaggctc agggcggccc gcggcacctg ctggcggaag
    10081 gcgagggcca tctcgatcag cggagggaac tcctcgggct tcatccgctt cctctcgtag
    10141 gcgaggatcc aggcccccac gatctcgaac tcccacttcc gggggaggcc gagcagccac
    10201 ttcatcatgc ggccgtcgat gaacgcgtag gcgaagtgcc ggtccttcgc cttgacctgg
    10261 aagcgcttgt tgaactccgg tgactcgaac tcgatgtcgc ggaacccgag tccgtcggcc
    10321 atgcgagtga acacgttctc gcgggtgatg gcgaggggcg agaacgcggc cgggatctcg
    10381 agcagcgcgc acgtgtggcg gtagtaggtc ctggtgcggt tccctttgct gtcgctcgac
    10441 tccgtgtagt accagtagtc gaatgcccgg accgggctgc cgcgccagtc tcccgagagg
    10501 acgttctcga ccttgcgccc gtcgcccttc gtgaagaggc ggaacgggag gttgatcgtg
    10561 ccgtaggggt cgtggcgcga gtaggtcatg ccatgcgagc gagcgaagag cgcgaacgcc
    10621 tcccgccgct tgtgggagaa gtaccagctg aggcccccca cgacggcgac acctaccagg
    10681 ccaaggatga acatcaggac gggatccacg cgctaagtat cggagcgggg gacgtggagg
    10741 taaagggatg gggggcggtg ggggcggggt cacgcccttc ctcagcgacg cttccggatc
    10801 cgcacgatag ggcgcaacag agccacttcc ttgtgcatct gttcatgcgg ggaggcggac
    10861 gtcggctgtc ggctggaaag aggaggcgaa gatgcggagg ctgttgcgac ctcgcgatct
    10921 ggcgcgttcg agcagcacgg gaaaggggtt gggcgcactc gcgctggggc ttcttgtcgc
    10981 ggcggcatcc tgcgggggag acggatacga ggtctccgag ctcccggcga tcgtgctcca
    11041 agcgagcgaa gccccagagg gaaccgtgca tgagcgagat gagtcaggga cgcgtgccct
    11101 gcgcgaattc tccgtcagcg gccaggagag gctgcagggc gaggggttcg tcgacgggta
    11161 tgcgacggtc ttcggcccaa ccggcggggt aaccacccat ctcgaagtta cgtctggagc
    11221 gatggtcttc gaggacggca gcggggccga ggcgagcatg gcagtcgtcc tccaggatgt
    11281 cgagggccga ctgatcgagg cgacggagat gtcggcggac gggctgggag ctgagtcggc
    11341 gggtctttcc ggccccgtgg agggggacgc caggcaacgg agtgcgctcc tctacgtctg
    11401 gaggaccgac aaccttgtcc tgttcgtgat cgcctcggct ctcctcgaga gcgcgccgag
    11461 ccctgaagtg atcctgggtt tggccgagac gatggactcc cgggcatcct gaggggatct
    11521 caatcagccg ggcgattcag ccgccaagcc gacggggggc gctggcgcgc cccccgtggc
    11581 ccttaccgat gcgcctagat gcccagctct gcgaacactt tctgggcgcg ttccagctga
    11641 acctggatct cctcgcccac ggtcagcatc tcgaccgtga agtgctccac caggtagctg
    11701 gtcttcgtga gcaggaccaa gtactcctcg tcttccatca gctccaggac cttctctcga
    11761 ggccccttga tcacgatgaa ccccgcctcg gcctcgaggt ccgtcgtcct ggagaagtag
    11821 ggctcgaaga aggtgatcac accctccgcg agcttcttcc cgaagaactc aatcgccgac
    11881 gcgaagtgct cgatgccggc gcgctcccgc ccaggcacca gcttccccca gcggtacatc
    11941 aggccgatct catgcatctc tgtcatctcc ccgctcagag tcggcccgcc gggtccccag
    12001 catcacctcc ccgacccgtc gggaacggtg gcccacgagg gacgattctg ggccgagtgc
    12061 cccggggggg atagggtcga agggccccag ccggcaggga ccaggcgccc cccgaaggtg
    12121 ctactccggc caggaagctc gagacgtcct cggtcacctc cagcgtcagt cgccgctccg
    12181 cttgacgccc gctgacggca ggccgccggc ggccccgccc cccaaccggg tccacgtgcg
    12241 ctcccgactc cctcgcctgg cttcttcccg catatacggg gatgcctgga gaggtcgaca
    12301 ctaaggaggg ggttcgcatg gctatgttcg cgcgcgtgac gagcgtcgac ctagagggtg
    12361 atatcgatca agccatcgcg ctcatccgcg accgcatcgt tcctcaggcc agtcggctag
    12421 aagggatcgt cgccgcgtac tggctcggca accggaacac tcgccgaggc ttagcggtca
    12481 ccgtctggga gagcgaagaa gccatgctcg cacggacgtc gcacggggag cagcgaggag
    12541 agaacggcag gaggcggaag tggacacgac tgcagtggag cactacgagg ttgtagcgca
    12601 tctctagcgt gcgtccccaa ccagcccgac aggccgcccc aggggggccc gccggttggc
    12661 ggcgggcctc ccctggtgcg ggttcgacgc ccttcgctcg ggacgagcta tctcccgctg
    12721 ggccggacga gcctccagac cttcccggtc gctcccgtgg ggccggttcg ctcggtgccg
    12781 agcacgtaca cgtcacccgc gctgtcctga ccgaacccga ggaggaagtg atccagcctg
    12841 cccgtggggc tggtggcgac gcggagctcc tggatctgcc acagtccctt cttcctcgac
    12901 ttggccacca gcagcgtgcc gtccggattc ccgaacgacg tgctccagtc cccgaacacg
    12961 taccggccct ggagctgcgg cagcgtcgat gaccggtaga cgaacccgcc gacgaccacc
    13021 aggccgatcc ctcccggctg tcggccgttc gcgtactcga tcaccggatc gacgagcgga
    13081 tcgccggccc ggggcccgga cagcacctcg tcggggcatt ccgccggcga ctccgtgttg
    13141 ttctcggcgt cgaagcagtg cgtgccttcc ttgacgttcc agccgaggtt cgccccgcgg
    13201 gtgacgaggt tgacctcctc ccacagcccc tgcccggcgt ccgatacgaa caggtccccg
    13261 gtcgccatgt cgaaggacat gcggtagggg ttgcggaggc cgtaggccca gatctcgtcg
    13321 agcccgaccg tccccacgaa ggggttgtcc gcggggatcc cgtacggatc tccgccgtcc
    13381 acgtcgatcc gcaggatgtt gccgagcagg ttctgctcga cgtcctgccc gttgccccca
    13441 gcgttgtcgg cgtaccagtc ctccacgtgg ccgaggccga cgtcgttcgc tccaccaccg
    13501 tccccgagcg agatgaacag gtagccgtgg gggccgaagg ccaatgcgcc cgcgttgtgg
    13561 ttgaactggg gctggtcgac ctgaagcaag atgcgctcgg aggacgggtc ggctcgatcg
    13621 gcgtcgtccg acacggtgaa ctccgagatg tgactggtgt ggttgaagcc cgccggggct
    13681 ccctcccgga gcggggcgct gtagtagacg aagaagcgtc cgttggaggc gtattcggga
    13741 tggaacgcaa gcccgagcag ccctcgctcg tcgaagccgg cctgcagggc gaccatccga
    13801 tcacgaacgt cgaggaacgg ctcggcgagg agctcgccct ccgacgtgat gatccggatc
    13861 aggcccgcct ggtccacgac gaacaaccgg ccggacccgt caggggcttc catgagagcc
    13921 acgggggagg tgaggccctc ggcgatcagc tcgagcccca cgtccatggg atcgcccatc
    13981 ggcgactgag cgccggacgg cgcggcgggg agcacgctca acgagatcgc gagcaacgct
    14041 cccagcatcg gggcgaagcg tcgggtacgc atggttcctc cttcctaggg cggtctcgga
    14101 tcgggaggag gctccgcgcg gggggtctct gccccggtat ccgggcatat gcgtccttga
    14161 agatcgagct cgctcgggac ggtgggcgtc cgtctcccta gtactactcc ggcctcggcc
    14221 cgacggttca ctcggatccg gggcgagctc agggcgagga tctcggatag gggcgcggcg
    14281 gatcctctcg gtgagctggc cgatctcctc gtagcccatg cagcttgtca gcagacttga
    14341 cacctcgggc ctccggttgc aagggtggca ggcaaagagg tggaaggggg catttgggat
    14401 gagatcgcga gccgcatcgt cgatactcgt ggcgctggcc ttggccatcg ctccggtccc
    14461 gggagcgtcg gcggaaccgc cggccgtatc gaggggattg gagttcgtgg gggcaaccga
    14521 ccttggcctc ccgtttccca ccatgaatac agagatcgag atccgaggcg accacgtcta
    14581 cgtggggagc ttcttcccgc tggatcagaa cggacagacc gctttcaagg tggcggatgt
    14641 ctcggatccc gccaacccag cgctcgccac ccacttcatg ttgcccggta ccgtccttga
    14701 tacccaggtg aacaacgatg tcgcacggtg cggctacggc aagaatctgg ctggtctgaa
    14761 cgatccggcg tgcaacaccg tcctggccac tacccaatcc gctcctggcg acgtcgacgg
    14821 cttgtggttg ttcgatgttc gcgaccccgc gaacgcgcag ccctggggtt gggactcgac
    14881 caagtcgttc gttgaccagg ttggattcat ccagcggccg gctcgcggct cccacaacgg
    14941 cttcctgttc ggagacttcg ccttcgccgc tggcatcgga gaggcgagct tcgagatctg
    15001 ggacatctcc ccgctgttca gcgatccggc ggcggcaccg accctcgccg cgacctactc
    15061 gaacctggcg gatcccaggc ctgtgcgccc ccagagggag cgggagggat cgcacgacat
    15121 cttcgtccag cggaacccgg acgggcggat cttcgcctac gtcgccggcc caaacttcta
    15181 catcgttgac gtcaccgacg tggtcgaggg ggccacgagg ggggacatca gcgaccacct
    15241 cgtgggcttc aacaattacg tgaacgccga cggcactctg accgtgacgc gtccgcacgg
    15301 cgggcactac ggtgagccaa ccgacgacgg cgatcacgcc tatgtgggcg acgaaactgg
    15361 atgcggagag cccgggatca tccacatctt ggacacgtcc ggtctgccgc aggtgggggg
    15421 gacgcccaag cagctggctg aggtagggac ggtcacgtgg cccacatcca acgtccccca
    15481 gtgcgcgaat cggaacgcgg acgattctgc tgatgacccc cagaaccgga acggggacac
    15541 ccagaacttc tgccggacgg gccacaactt ccggatctat ggggacatca tgacccacgg
    15601 caactactgt gagggcgtct tcgtgtggga cctcacggac cgcgcgaacc ccacccttct
    15661 cgcacagcat cgcagcgtcg gggactacgg agggaagcgg aaggagctga aggaccacgc
    15721 ggcgaaggcg gacctgcact caaccttctg gcaggccgtc tcggacggcg atcccgacgc
    15781 gctggtgcgg ggcgagcagt tcatctacgg gtctgacctt tcggcgggcc tcgtcgtgct
    15841 gcggatgcgt cccgcgggga gcggatagag cagcctgcgg tgaaggggag ggacgccacc
    15901 cgagcccggt cctcagggac ctcctcgcag gatgcggggt cgttgtagaa agacgctcgg
    15961 agacgacggt cggtgctgtg gccaacgacg tcgagcgacc cggggtggag tccagcagct
    16021 cgctctcctt gacaggctct cccctcggcg ggatcaccgc cgaggacgcc ggcacggggt
    16081 tctggttcgt gcgcttcccc aagaaggtta cggggaagtc gattttcgtg agtctccagg
    16141 gcaagaacat cagtcacggc tcgatcaacg ccgtgccgtg cggagggggc gtcggcaacg
    16201 ctcgggcgac gtcttgtagc gcgaacgata acggcaacca cgtcctggtc ggcacgtacg
    16261 acgagaacgg ggaccccgtc gaccggccct tctacatcat ggcggttggc tagcgcgtca
    16321 ccacatcatg gtggtcgtct agcgcgtcac gctgccggac cagaagccgc tcccggcggg
    16381 ggagcttccg accgtccgct agcgagctcc ctcctcctcg atcccccaag cgggcacgcc
    16441 gaggatcagg ttgagcgcac cggcgatggc caggccgagc cccgcgccac ccaccacatc
    16501 gagcgggttg tgcgcgccca ggtacacgcg tgcgaagcag accaggccta cgagcgccca
    16561 cggcagcgcc ttccaggctc ctcgcaggta cggggtgacg accccggcga gggccgccgt
    16621 gagcaccgcg tggccggaca cgaacgcgag cccacggatg ggcaccccgc gggcgatggc
    16681 gtcgggggtg gacacggccg gtcgggcacg ctccacgacc agcttcacga cccgctccag
    16741 cgccagcttc aggaccgtgg ccaggagcgc cgccgcggcg agtctcggct tccggaaccc
    16801 gagggcgatg accgcgacgg ccggtccgac cagcaggacg cccaggatct cggcggcctg
    16861 catcggcacc ctgagccacg agggaaggcc gttgatcgcg tggaagaccg cccgctccca
    16921 tccgggaacg gtcgcgccgc tcgccaccac ccccgtcagc aggagcgtgc cgagaccggc
    16981 cacgatccag acggcatcga ggcgacgccg cctgacggga ggccgggaag gtggagcccg
    17041 cacaagggca ccttatccgc cgctcgccag acctgctctt acccgcgccg cacgatgagg
    17101 ccgtcgccct ggcggtccgc gactacgtga tcgccgtcgg tgatctcccc ctccagcagc
    17161 gcgagcgcga gcttgtcctg gatctccttc tggatcaggc gcttgagcgg acgggcgccc
    17221 aaggccgggt cccagccccg ctccgccagg aagctccgcg cgtcgcccgt gagcttcagc
    17281 gtcagccgtc gctcggccag ccgctccgcg aaccgccgca cttggatgtc cacgatctcg
    17341 gcgatctgct ccttgtcgag cggctcgaag acgatcacgt cgtccacacg gttcagaaac
    17401 tcgggccgga aggcctcgcg catctccgcc agcacgcgct cacgtcgatc ctccggcgag
    17461 agcgtctcct cgaagaacca ccggctcccc aggttcgagg tcatgatcac gaccgcgttc
    17521 ttgaagtcga ccgtgcgccc ctgcccgtcg gtgaggcgcc cgtcgtcgag cacctggagg
    17581 agcaggttga acgcgtccgc gtgcgccttc tcgatctcgt cgagcagaat caccgagtac
    17641 gggcggcggc ggacggcctc ggtgagctgg ccgccctcct cgtagcccac gtagccgggc
    17701 ggcgccccga tcaggcgggc tacggcgtgg cgctcctggt actcgctcat gtcgatgcgc
    17761 accatcgccc gctcgtcgtc gaagaggaac tcggccaggg cccgggccag ctcggtcttc
    17821 cccacaccgg tgggcccgag gaagatgaac gatccgatcg gacggttcgg gtccgagagc
    17881 ccggcccgcg agcggcggat cgcgttggcc accgccgtta cggcctcgtc ctggcccacg
    17941 acgcgctcgt ggagggcctc ctccatccgg acgagcttct gcatctcgcc ctccaggagg
    18001 cgcgagaccg gcacgccggt ccaggctgag acgacctcgg ccacgtcctc ctcgtccacc
    18061 cgctccttca gcatcttctg gtctgactgg atctcggcca gcgccgcgtt ctcggcctcg
    18121 agctccttcc gcagctcgac gatccgcccg tagcggagct cggcggcgcg ctcgaggtcg
    18181 ccgtcgcgct cggctcgctc ggcctggccc ctcgtctcct cgagctccga cttcagctcg
    18241 tggatccgcg agatgtgctc cttctcgcgg tcccagtggg cccgcatggc actgagttgc
    18301 tcgttcaggt cggccagctc ctgctcgatc ttctccaggc gctgtcgcga cgcctcgtcg
    18361 gtctccttct tcagcgcggc ccgctcgatc tcgagctgct tgacgcggcg gtccacctca
    18421 tcgatctcca cgggcatcga gtcgatctcg atccggaggc ggctggcggc ctcgtccacg
    18481 aggtcgatgg ccttgtcggg gaggaagcgc cccgtgacgt agcggtcgga cagcatagcg
    18541 gcggccacga gcgcctcgtc cttgatctcg acgccgtggt ggacctcgta gcgctcgcgg
    18601 aggccgcgca ggatcgcgat cgtgtcctcg accgagggct cgccgacgaa gaccggctgg
    18661 aagcggcgct ccagcgcggc gtccttctcc acgtgcttcc ggtactcgtc gagggtggtg
    18721 gccccgatca tgcggagctc ccctcgggcc agcagcggct tcagcatgtt gccggcgtcc
    18781 accgcgccct cggcggcacc ggcccccacg atcgtgtgga gctcgtcgat gaacgtgatg
    18841 atctgtcttt cgctggcggt gatctcctgg agcacggctt tcaggcggtc ctcgaactcg
    18901 ccccggtact tggcgccggc caccatcgcc ccgatgtcga gggccacgat gcggcggccc
    18961 ttcagcgact cggggacgtc gccggccacg atgcgctggg ccaggccctc ggcgatcgcc
    19021 gtcttcccca caccgggctc gccgatcagc acggggttgt tcttcgtgcg gcgcgacagg
    19081 acctggatca cgcggcggac ctcgtcgtcg cgtccgatca ccgggtcgag cttgccgcga
    19141 cgggccaggt cggtcaggtc gcgcccgtag cgctccaggg cctcgtactt ctcctcgggg
    19201 ttctcgtcgg tgacgcgctg acggccgcga acctcggcca gcgcggcgag gaggcggtcg
    19261 cggtcgaggc cgagctcccg gaggagcgag ccgagcgggc cctggtcggc gagcatggcg
    19321 aggagcaggt gctcggtcga gacgtactcg tcggtgaggg cctccgcctc gtcggcggcg
    19381 ccctccagga cgcgcgcggt ggccggggcg agtcgcgcct cctgcgtggt gccgtagacc
    19441 ttcgggatct ggtcgaggcg ctcctcgact cgccc
//
LOCUS       DASUTW010000178         8113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1221564, whole genome shotgun sequence.
ACCESSION   DASUTW010000178 DASUTW010000000
VERSION     DASUTW010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8113
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221564"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VF058_06140"
     CDS             <1..451
                     /locus_tag="VF058_06140"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX6207925.1"
                     /translation="VPDVVGLTEEEAMRVLGEAGFTAQATEVDSSEPAGTVVGQSPQG
                     GAKVPVSTTVTIQVSTGKAPRARVPDVVGMSEGQAIGALERAGFAAAVSYETTNRKNL
                     DGIVGAQAPGAGTRAKEGATVSITVYRHEKPKPKPKPSPTPEPSPTA"
     gene            complement(452..901)
                     /locus_tag="VF058_06145"
     CDS             complement(452..901)
                     /locus_tag="VF058_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018397810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX6207926.1"
                     /translation="MGALKDRLSTELREAMKARDKTRMATIRLLMTAVREREVEVGHE
                     LSDEELHEVVSTQAKRRKESAQAYDEGARPELAEQERAELAILQNYLPEQLSAEELDA
                     IIDEAVSATGASGPDDLGKVMGSVMGRVKGRADGSEVRRRVAERLEG"
     gene            914..1780
                     /locus_tag="VF058_06150"
     CDS             914..1780
                     /locus_tag="VF058_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6207927.1"
                     /translation="MLWWILLGLLAAGLLVAAYAILIERRWYRLARYRLDILPGGGSL
                     TILHLSDLHMIDGEAHKRRFLESLPRADVTIVTGDLLGEPEAVEYVVESLRPLRGRLA
                     SLFVLGSNDIYVPRPMNPLRYFVPHEKRRRRRGRRGRAPDLVNQLEADGWVHLRNLRR
                     DGSLDGVPMEILGLDDPHIHRGNVSLSPRRQADRFGLAVVHSPDPAPELAALGWDLIV
                     TGHTHGGQVRLPWGRAIVTNSSIPRRMASGLHRIGGSFLHVSPGLGTSKYAPFRLFCR
                     PEATVLDLRSGG"
     gene            complement(1782..2282)
                     /locus_tag="VF058_06155"
     CDS             complement(1782..2282)
                     /locus_tag="VF058_06155"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX6207928.1"
                     /translation="MADVKTSIEKAVAYLTEHPEEARYTDSAATATLGDALRVEVEGP
                     AGERLTTDMPGGVGGRGEHPTPGWLFRAAVASCVASTVGMEAAREGVDVGDAEVVVDS
                     ESDDRGMLRIDDGVPAGPLSMRIRISLRGGEVSDRAAEVAERGAARCPVCDATKRAVE
                     VSVEVG"
     gene            complement(2275..3318)
                     /locus_tag="VF058_06160"
     CDS             complement(2275..3318)
                     /locus_tag="VF058_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6207929.1"
                     /translation="MRIAQVSPPWVAVPPKGYGGIEWVVSLLADGLAERGHDVTLFAT
                     GDSQTKARLEYVFEAAPGSTAINSIWHDTVHQMLVHRDLGRFDVLHHHTPFSALVAAM
                     CHDVPVVHTLHGDFTPQFRLLYEQVADRLTFVAISESQRAAMPELRYGGVVYNGIDLG
                     LYPFRSEKEEFLCFIGRANPDKGAHRAVLAARAAGMPLVMAVKMAHPVEREHWTREVE
                     PLLGPDVTVLGEISVEEKADLMSRARAVLFPIDWPEPFGLVMTEAMACGTPVIATPRG
                     AVPEVIADGRTGFLVSVEGYPEEAAGAIGRLGEIDPEACRSHVAERFSKESMVEGYER
                     VFERVVEGGRSGG"
     gene            complement(3329..3523)
                     /locus_tag="VF058_06165"
     CDS             complement(3329..3523)
                     /locus_tag="VF058_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207930.1"
                     /translation="MEPRDCTWCGSARSVEHDVCQVCLMRFPELKGADEVVVILEERE
                     LQIHAVPAEEEKATGTAVAE"
     gene            3596..4978
                     /locus_tag="VF058_06170"
     CDS             3596..4978
                     /locus_tag="VF058_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEX6207931.1"
                     /translation="MAATANDDLLPQDVLFQLDEIGSADILVGIPCFNNEETIGGVIR
                     AVEAGLRKHFPEKRSVICVSDGGSADRTVAVAQSAEIGEDWKLLLVPGDSPVPKRIAF
                     PYSGLSGKGSAFRSIFEVAARLGVAACGVVDSDLRSITPYWLDRILSPIVHYGYEYVA
                     PIYARHKFDGTITNCIAFPVTTALYGVRIRQPIGGEFGLSGELAKIYAAKNVWESDVA
                     RFGIDIWMTTVALVEGAKMSQGILGAKLHDAKDPGKDLGPMFRQVVGSLFALAGRYRN
                     DWWDIDTATSPPIFGFPAAYSAEPIKVSVPRLTWKFVEGYVKHQALWTQVLSDESLNG
                     VQTAVGDAAETTRGLVLDVDTWTKIVYDFLIAYNARRVDPGTLIDSMIPLYFARTATF
                     IQEVSDADLHEAEDRIQANADAALALKPYLKRRWREEGIPERELSEQEVPMEGESPEG
                     MVGALEQQNK"
     gene            5027..5100
                     /locus_tag="VF058_06175"
     tRNA            5027..5100
                     /locus_tag="VF058_06175"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5061..5063,aa:Pro,seq:cgg)
     gene            5326..5724
                     /locus_tag="VF058_06180"
     CDS             5326..5724
                     /locus_tag="VF058_06180"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEX6207932.1"
                     /translation="MIDLNYAALAAAVASAFVISTTYYIVLGRQYAEARGLSPEAAEA
                     RPPAWKILVELARSLVVAAVVAGLAGLLETPDLGDAAGLGLALWIAFPVVLLVGSVTW
                     DAARPRVAAIHAGDWLVKLVVIASIVGLWR"
     gene            5749..6039
                     /locus_tag="VF058_06185"
     CDS             5749..6039
                     /locus_tag="VF058_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HEX6207933.1"
                     /translation="MRISELQDLMRRTYLDRDRRRGVDGTFRWLVEEIGEVARALRQD
                     DREALRHEVADALAWLTSLANVAGVDLEEAMSRYADGCPKCGEIPCACPFRA"
     gene            complement(6036..6416)
                     /locus_tag="VF058_06190"
     CDS             complement(6036..6416)
                     /locus_tag="VF058_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013806925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6207934.1"
                     /translation="MRTVDYVIRYVEDLDRSVAFYRDVLGLEHRLTDEAYAEFSAGGT
                     KLGFFPRSSLPDLLGREARSGGSPDGEIVLLVEDVDAEADRLRGAGVEILTGPVERPW
                     GHRTVHLLDPDGLVVELAQEIERT"
     gene            6458..7381
                     /locus_tag="VF058_06195"
     CDS             6458..7381
                     /locus_tag="VF058_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6207935.1"
                     /translation="MAKLIQTAHGTRSEAFGPVEWGLLASIALMWGSSFLWIAIGLEA
                     FEPGLIAFLRVALGASFLGLTSRARRGIEREDWPRVALLGLVWVAIPFTLFPVAQQWI
                     DSSTAGMINGAMPLFTAVFATLLLRTMPGARQLIGLAVGFGGVILVSLPAAGEGGSRL
                     LGIGLVLLATVLYGLAANLAVPLQQRYGALPVVWRAQLASVVAMLPYGLISLPGSSFR
                     LSSLGAVLILGFLSTGVALVAMAVLVGRAGATRGALAIYFIPVVATILGVVFRDETVE
                     PVVFAGIGLIIAGAWLASRRERRRGSVPAPA"
     gene            complement(7378..>8113)
                     /locus_tag="VF058_06200"
     CDS             complement(7378..>8113)
                     /locus_tag="VF058_06200"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014910675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX6207936.1"
                     /translation="EINTDVAGVSTADPRIVPEARKLHAVSYEEMLEMAATGAKVLEL
                     RAVEYARNSGIKLHVRSSFDSEGGTWIAEEDERMEQAIISGVTHDTSQAKVTLVDVPD
                     RPGIAATIFKAVADEGLNVDMIVQNVSREGITDVSFTIPKDDLPHAGKAIGRVAEEIG
                     VAYDTDADIAKISLIGAGMKTHPGVAARMFDVLAEAGINLEMISTSAIRISCVIRASD
                     VEKAVRVLHDEFRLSEEDVVREHEGA"
BASE COUNT         1114 a         2942 c         2793 g         1264 t
ORIGIN      
        1 ggtccccgac gtcgtgggct tgaccgagga ggaggcgatg cgggtgctgg gcgaggccgg
       61 cttcaccgcc caggccaccg aggtggactc gagcgagccg gcaggcacgg tggtcgggca
      121 atccccgcag ggcggagcca aggtcccggt gagcacgacc gtgacgatcc aggtctccac
      181 cgggaaggcc cccagggcca gggtgcccga cgtggtcggg atgagcgagg gccaggcgat
      241 cggggccctg gagcgcgccg ggttcgccgc cgccgtctcc tacgagacga cgaaccggaa
      301 gaacctcgac gggatcgtgg gcgcgcaggc ccccggcgcc ggcaccagag ccaaggaggg
      361 cgccacggtc tcgatcacgg tctaccggca cgagaagccg aagcccaagc cgaagccgtc
      421 gcccaccccg gagccctccc cgacggcctg atcaaccctc gaggcgctcc gccacgcgcc
      481 ggcggacctc gctcccgtcg gctcgaccct tcacccggcc catcacgctg cccatcacct
      541 tgcccagatc gtcggggccc gaggcgccgg tcgccgacac ggcctcgtcg atgatcgcat
      601 cgagctcctc ggcgctcagc tgctcgggga ggtagttctg gaggatcgcc agctcggccc
      661 gctcctgctc cgccagctcg gggcgggcgc cctcgtcgta ggcctgggcc gactctttcc
      721 tgcgcttggc ctgcgtggag acgacctcat ggagctcctc atcggacagc tcgtgcccca
      781 cctcgacctc gcgctcgcgg accgcggtca tgagcaggcg gatcgtcgcc atccgcgtct
      841 tgtcgcgggc cttcatcgcc tcccgcagct ccgtcgacag ccgatccttg agggctccca
      901 tacaatcgcc tccgtgctct ggtggatcct gctcggcctc ctcgccgccg gtctcctggt
      961 ggccgcctac gccatcctga tcgagcgtcg atggtatcgc ctcgcccgct accgcctcga
     1021 catcctcccg gggggcggat cgctcacgat cctgcacctc tcggacctgc acatgatcga
     1081 cggcgaggcg cacaagcggc ggttcctgga gagcctgcct cgggccgacg tcacgatcgt
     1141 caccggggac ctgctcgggg agccggaggc agtggagtac gtcgtggagt ccctccgccc
     1201 cctgcggggc cggctcgcgt cgctcttcgt cctgggctcg aacgacatct acgtgccgcg
     1261 cccgatgaac ccgctccgct acttcgtccc gcacgagaag cgccggcgcc gccgggggcg
     1321 ccgcgggagg gcgccggacc tcgtgaacca gctcgaggcc gacggctggg ttcacctccg
     1381 aaacctccgc cgggacggga gcctggacgg cgtgccgatg gagatcctcg gcctcgacga
     1441 cccccacatc cacaggggga acgtgagcct ctcgccccgc aggcaggccg accggttcgg
     1501 gctggccgtc gtgcattccc ccgacccggc ccctgagctg gcggccctgg gctgggacct
     1561 gatcgtgacc gggcatacgc acgggggtca ggtccggctg ccgtggggcc gggccatcgt
     1621 cacgaactcc tcgatccccc gccgcatggc cagcggcctc caccggatcg gcgggtcgtt
     1681 cctccacgtc agccccgggc tggggacctc gaagtacgcg ccgttccgcc tgttctgccg
     1741 ccccgaggcc acggtgctcg acctgcgctc gggcggctga ctcagccgac ctcgactgag
     1801 acctccaccg cccgcttcgt cgcgtcgcac accgggcacc gagcggcgcc gcgctcggcc
     1861 acctcggccg ctcgatccga gacttccccg ccacggagcg agatgcggat ccgcatcgag
     1921 agcggtcccg ccgggacgcc gtcgtcgatc cggagcatcc cccggtcgtc ggactcgctg
     1981 tccaccacga cctccgcgtc ccccacgtcg acgccctctc gggcggcctc catccccacc
     2041 gtcgacgcca cgcacgaggc gacggcggcc cggaacagcc agcccggggt ggggtgctcc
     2101 ccacgtcccc ccacgccccc cggcatgtcg gtggtcagcc tctcgccggc cggcccctcg
     2161 acctccaccc tcagcgcgtc acccagcgtc gccgtcgccg ccgagtccgt gtagcgcgcc
     2221 tcttccgggt gctcggtcag gtaggcgacc gccttctcga tcgacgtctt cacgtcagcc
     2281 accggatctc cctccctcca cgacccgctc gaacacccgc tcgtagccct cgaccatcga
     2341 ctccttggag aagcgctcgg ccacatggct gcggcacgcc tccgggtcga tctcgcccag
     2401 ccgcccgatc gcaccggcgg cctcctccgg atacccctcc acgctcacca ggaaccccgt
     2461 ccggccgtcg gcgatcacct cgggcaccgc gccgcgcggg gtggcgatca ccggcgtccc
     2521 gcacgccatg gcctcggtca tcacgaggcc gaagggctcg ggccagtcga tcgggaacag
     2581 gacggcccga gcgcgggaca tgaggtcggc cttctcctcc accgagatct cgccgagcac
     2641 ggtgacgtcg ggcccgagca gcggctccac ctcgcgcgtc cagtgctccc gttccacggg
     2701 gtgggccatc ttcacggcca tcacgagcgg catcccggcc gcgcgagcgg ccagcaccgc
     2761 ccgatgggcc cccttgtccg ggttggcgcg cccgatgaag cagaggaact cctccttctc
     2821 ggaccggaac gggtagagac cgaggtcgat cccgttgtag acgacgcctc cgtaccgaag
     2881 ctcgggcatg gcggcccgct gggactcgct gatcgccacg aacgtgaggc ggtcggccac
     2941 ctgctcgtag aggaggcgga actgcggcgt gaagtcgccg tggagcgtgt ggaccaccgg
     3001 gacgtcgtgg cacatcgcgg ccacgagcgc cgagaacggc gtgtggtggt gcagcacgtc
     3061 gaaccgcccg aggtctcgat ggacgagcat ctgatggacg gtgtcgtgcc agatcgagtt
     3121 gatcgcggtg gacccgggcg ccgcctcgaa gacgtactcc agccgggcct tggtctgcga
     3181 gtcgccggtg gcgaagaggg tgacgtcgtg gccccgctcc gcgagaccgt ccgccaaaag
     3241 ggacaccacc cactcgatgc cgccgtagcc cttgggcggg acggcgaccc acggtgggga
     3301 gacctgggcg atccgcatcc ggccgatgct actcggcgac ggcggtgccc gtcgctttct
     3361 cctcctcggc gggcacggcg tggatctgta gctctcgctc ctcgaggatc acgacgacct
     3421 cgtccgcgcc cttgagctcg gggaaccgca tcaggcacac ctggcagacg tcgtgctcga
     3481 ccgatcgggc gcttccgcac cacgtgcagt cgcggggctc catgggtcct ccgttccgag
     3541 cgtgcccagc agggcacccg gtatgctagc acctgaggtc gactcccagg aaggggtggc
     3601 agcgacggcc aacgacgacc tgctccctca ggacgtgctg ttccagctgg acgagatcgg
     3661 gagcgccgac atcctggtcg ggatcccctg cttcaacaac gaggagacga tcgggggcgt
     3721 catccgcgcc gtcgaggccg gccttcggaa gcacttcccc gagaagcgat ccgtgatctg
     3781 cgtctccgac ggcggatccg ccgaccgcac cgtggccgtg gcgcagtcgg ccgagatcgg
     3841 cgaggactgg aagctcctgc tcgtcccggg ggattccccg gtccccaagc ggatcgcgtt
     3901 cccgtacagc ggcctgtcgg gcaagggcag cgccttccgc tcgatcttcg aggtggccgc
     3961 gaggctggga gtggcggcat gcggggtcgt ggactccgac ctgcggagca tcacccccta
     4021 ctggctcgac cggatcctct ccccgatcgt ccactacggg tacgagtacg tcgctccgat
     4081 ctacgcacgc cacaagttcg acggcacgat cacgaactgc atcgccttcc cggtgacgac
     4141 ggccctctac ggcgtgcgga tccgtcagcc gatcggtggg gagttcgggc tctcgggcga
     4201 gctggccaag atctacgcgg cgaagaacgt gtgggagtcg gacgtggcga ggttcgggat
     4261 cgacatctgg atgacgaccg tggcgctcgt ggagggggcg aagatgtccc agggcatcct
     4321 cggcgccaag ctgcacgacg cgaaggaccc cggcaaggac ctgggcccga tgttccgcca
     4381 ggtcgtgggc agcctcttcg ccctcgccgg ccggtaccgc aacgactggt gggacatcga
     4441 caccgcgacc tcgcccccga tcttcgggtt ccccgccgcg tactcggccg agccgatcaa
     4501 ggtctcggtg ccccggctga cctggaagtt cgtggaggga tacgtgaagc accaggcgct
     4561 gtggacccag gtcctgtccg acgagtcact gaacggcgtg cagaccgccg tgggcgacgc
     4621 ggcggagacc acccgagggc tggtgctgga cgtggacacc tggacgaaga tcgtctacga
     4681 cttcctgatc gcctacaacg cgcggcgggt ggaccccggc acgctgatcg actccatgat
     4741 cccgctgtac ttcgcgcgga cggcgacgtt catccaggag gtctcggacg ccgatctcca
     4801 tgaggccgag gatcggatcc aggcgaacgc cgacgcggcc ctggcgctca agccctacct
     4861 caagcggcgg tggcgcgagg aggggatccc cgagcgcgag ctgtccgagc aggaggtccc
     4921 catggagggc gagtcgcccg aggggatggt cggcgcgctc gagcagcaga acaagtagcg
     4981 gcctggtggg ccggacggct cgcgctacac tggtgctccg gcccgtcggg acgtggcgca
     5041 gcttggtagc gcgcagcgtt cgggacgctg aggtcgcggg ttcaaatccc gccgtcccga
     5101 ctcagtgatg tcgcggggca tccccctgcc cgcgatgact tctcgctgcc ggagccgtct
     5161 ctccttcgac ggcgatccca cgccgaaccc cagacccgat gctcacgcca tcggctcgat
     5221 ggggcaaagg ggctccgcct cggggttgag cgatcagacg acccgtgggt cgatgatccc
     5281 cgggagcgtg agtccctgga tcatgcggcc ccgaggaggg tacggatgat cgacctcaac
     5341 tacgccgcgc tggccgcggc ggtcgcctcg gcgttcgtga tcagcacgac gtactacatc
     5401 gtgctcgggc gtcagtacgc ggaggctcgg ggactgagcc ccgaggcggc ggaagctcga
     5461 cccccggcgt ggaagatcct cgtcgagctg gcgcggagcc tggtcgtcgc ggcggtggtc
     5521 gccgggctcg ccgggctcct ggagacgccg gatctgggcg acgcggccgg gctcgggctc
     5581 gcgctgtgga tcgcgttccc ggtcgtcctg ctcgtgggct ccgtgacctg ggacgcagca
     5641 cgtcccaggg tcgcggcgat ccacgcgggc gactggctgg tgaagctggt cgtgatcgca
     5701 tcgatcgtgg ggctctggcg gtgatcgagg ggcactagac tgcccccgat gcggatctcg
     5761 gagctgcaag acctgatgcg gcgcacctac ctggatcggg atcgtcgccg cggcgtggac
     5821 ggcacgttcc ggtggctcgt ggaggagatc ggggaggtgg cgcgggccct tcgccaggac
     5881 gatcgggaag ccctccggca cgaggtggcc gacgccctcg cctggctgac ctcgctggcc
     5941 aacgtggcgg gcgtcgacct ggaggaggcg atgagccgct acgcagacgg ctgcccgaag
     6001 tgcggggaga tcccctgcgc ctgccccttc cgggcctagg tgcgctcgat ctcctgggcc
     6061 agctccacga cgagcccgtc ggggtcgagg aggtgcacgg tccggtggcc ccacggccgc
     6121 tccactggcc cggtgaggat ctcgacaccg gccccccgga gccggtccgc ctcggcgtcc
     6181 acgtcctcca ccaggaggac gatctcgccg tcgggcgacc ccccggaccg cgcctcccgt
     6241 cccagcaggt cgggcaggct cgaccgggga aagaagccca gcttcgtgcc ccctgccgag
     6301 aactcggcgt acgcttcgtc cgtcaagcgg tgctcgaggc ccagcacgtc ccggtagaag
     6361 gccaccgatc ggtccaggtc ctccacgtac cggatcacgt aatccacggt tcgcatgccg
     6421 ggaacatacc cggacagagg agggttcttg ctctgcgatg gccaagctga tccagaccgc
     6481 ccacgggacc cgctccgagg ccttcggccc cgtcgagtgg ggcctcctcg cctcgatcgc
     6541 cctgatgtgg ggatcgtcgt tcctctggat cgcgatcggg ctggaggcgt tcgagccggg
     6601 gctgatcgcg ttcctccgtg tcgcgctggg ggcctcgttc ctggggctga cgtcgagggc
     6661 ccgccgcggc atcgagcgcg aggactggcc ccgggtcgcg ctcctagggc tggtgtgggt
     6721 ggccatcccg ttcacgctct tcccggtggc ccagcagtgg atcgactcct cgaccgcggg
     6781 gatgatcaac ggcgcgatgc cgctgttcac cgccgtcttc gccacgctcc tcctgcggac
     6841 gatgccgggg gcccggcagc tgatcggcct ggccgtgggg ttcggggggg tcatcctcgt
     6901 gtccctccct gcggcgggcg aggggggctc acgcctcctc gggatcgggc tggtcctcct
     6961 ggccaccgtc ctgtacgggc tggccgccaa cctggccgtc cccctccagc agaggtacgg
     7021 ggccctcccg gtcgtgtggc gggctcagct cgcctcggtg gtggcgatgc tcccgtacgg
     7081 tctgatctcg ctccccggct cctcgttccg cctctcgtcg ctcggcgccg tgctgatcct
     7141 cgggttcctg tccaccgggg tggcgctcgt ggccatggcg gtcctcgtgg ggagggccgg
     7201 cgccaccagg ggcgcgctgg ccatctactt catccccgtg gtggccacga tcctaggggt
     7261 ggtgttccgg gacgagacgg tggagcccgt cgtcttcgcc gggatcgggc tgatcatcgc
     7321 cggggcctgg ctggcaagcc gtcgggagcg ccggcgcggg tcggtccccg ctccggccta
     7381 ggcgccctcg tgctcgcgga ccacgtcctc ctcggagagc cggaactcgt cgtggagcac
     7441 gcgcaccgcc ttctcgacgt ctgaggcccg gatcacgcac gagatccgga tcgccgaggt
     7501 ggagatcatc tccaggttga tcccggcctc ggccagcacg tcgaacatcc tcgcggccac
     7561 gcccgggtgg gtcttcatcc ccgctccgat cagcgagatc ttggcgatgt cggcgtcggt
     7621 gtcgtaggcc acgccgatct cctcggccac gcgcccgatc gccttcccgg cgtgggggag
     7681 gtcgtccttg gggatcgtga acgagacgtc ggtgatgccc tcgcgcgaga cgttctgaac
     7741 gatcatgtcg acgttgaggc cctcgtcggc caccgccttg aagatcgtgg ccgcgatgcc
     7801 cggccggtcc ggcacgtcca ccagggtgac cttggcctgc gaggtgtcgt gggtcacccc
     7861 ggagatgatc gcctgctcca tccgctcgtc ctcctccgcg atccaggtgc ccccctcgct
     7921 gtcgaaggag gaccggacgt ggagcttgat cccgctgttc cgagcgtact ccacggcccg
     7981 gagctcgagc accttcgccc ccgtggctgc catctccagc atctcctcgt aggagaccgc
     8041 gtggagcttt cgggcctcgg ggacgatccg ggggtcggcg gtggagaccc cggccacgtc
     8101 ggtgttgatc tcg
//
LOCUS       DASUTW010000179         5034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1482366, whole genome shotgun sequence.
ACCESSION   DASUTW010000179 DASUTW010000000
VERSION     DASUTW010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482366"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VF058_06205"
     CDS             <1..724
                     /locus_tag="VF058_06205"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF013461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEX6207937.1"
                     /translation="SAVTKPSAYLENVYQDEQGKVNFFETSYTQNGRAVFGLEDLGDY
                     ADPRDLDTVDFMLILNRNENIIPAVARLTRTQAAAYFMLGETTGTSAGGKAEEGKFLR
                     VPGTNPFFPLRQDQHGNRILELLDSHPDMEVYLMNTGRVGGKDEHEGSKKVKIPHSSA
                     VVKGIAEGTISWAEDPDFGYEVAESVPGFDDEELLQPRRLYERQGRMDEYDAMVDRLK
                     TERVEFLKRFEGLDEEIIKAVG"
     gene            910..2994
                     /locus_tag="VF058_06210"
     CDS             910..2994
                     /locus_tag="VF058_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6207938.1"
                     /translation="MTVEPASRTEGGASKLRVAVWVLHVALPLLGLWLLLAQPDLDVA
                     WEDHLWHFWLVGVVAAINAVLGLRISEAARRHDDARLFVVGLAFLASAGFFGLHALAT
                     PTVLLTGANAGFQVASPVGLVVFAALAAVSALDLSGPTGEAIMRRQPLLQWAVIGLLG
                     AWAAVSLAGLPPLDAALDAEQARGPAALLTIAGVPLYGFAAGRYVALYRRRPSVMLIS
                     LITAFVLLAEALAAMPLARAWAASWWAWHVLMLAAFGFIAYSAHVQYRREGGGTALFN
                     AISLRETVRRIREEHAQALEELVEAMRAQAETGAPDAVHPTAARLADRFGLTEGQVEV
                     LERAAEALAGEREQTARLAALVALGRESRVMLDEEALLGRALDLSGGVFRADRLGIRL
                     VQGGRLRPLRGDGTPARAAAEAAQRLETIEGSEGEERVLAFPLTVKDRAAGVLEVRRR
                     GRGFHERERSVLESLAVQLSIALENVRLYAQIDALFRQYLSPDVATALLADPDQASLG
                     GGLTEVTVLFGDLRGFTSFSERTDPGAVVELLNAYFGAAVPLVLARGGTVDKFVGDAI
                     MALWGAPTRQLDHARRAAAAALAIQGAIEDLPGDPDRPRFRIGINTGTALVGNIGSDE
                     LRNYTAIGDTVNLASRLQELAEPGRIAIGEATLQALGEGAVVEPLGEVRVKGREAPVR
                     AYLLEDLLTGPG"
     gene            complement(2978..3271)
                     /locus_tag="VF058_06215"
     CDS             complement(2978..3271)
                     /locus_tag="VF058_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207939.1"
                     /translation="MTATLDVLAWVLAGAFFLIGVRAGFDGTQLEGRLSGTDRLLIAV
                     HDAARAGFWLALAGLFVGFALVDDPYAFRWFGMIPISMAALRMLAAVGLSRGR"
     gene            complement(3268..3924)
                     /locus_tag="VF058_06220"
     CDS             complement(3268..3924)
                     /locus_tag="VF058_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX6207940.1"
                     /translation="MDEGFGLPSGGFADRTEAGRLLAEALEGLEDDVVVLGIPRGGVP
                     VAAEVARARGWPLDVVIPRKIGAPGNPELGLGAIAPGVRVLDEGIVARLGVPQEYLDR
                     EIERQEAEIRRRAEAYRRGRPPASVAGKTAIVVDDGVATGGTAAAALRWARAQGAEAV
                     VLAVPVGAPESIRRLSREADRVVVLAAPERFYAVGQWYRDFEQTSDEEVVRLLDEARA
                     "
     gene            4009..4242
                     /locus_tag="VF058_06225"
     CDS             4009..4242
                     /locus_tag="VF058_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX6207941.1"
                     /translation="MVNAYILIQTEVGKAAQVAKEVAAIDGVTSAEDVTGPYDVIVRA
                     EAGNVDDLGKLVVARIQSVEGITRTLTCPVVHL"
     gene            4340..4492
                     /locus_tag="VF058_06230"
     CDS             4340..4492
                     /locus_tag="VF058_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207942.1"
                     /translation="MRECDGCRSEMRVVDSYVALEGAQEVEVIRWRCPRCGEESESTD
                     RLLEVA"
     gene            complement(4493..>5034)
                     /locus_tag="VF058_06235"
     CDS             complement(4493..>5034)
                     /locus_tag="VF058_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX6207943.1"
                     /translation="WLNVPHDCGFAFLREPSRLGRVFSVAAAYLPPPDEERTVYFNLG
                     PESSRRARGFTVWATLRAYGREGYRGIVERHLDLAQRVARKVEEAPDLELLAEAQLNI
                     VCFRYRPPGVPEEDLDELNERIGREAMADGRLYVGTTTYEGKAAFRPAIVNWRTSEED
                     VDLIPDVVRELGERIRGEA"
BASE COUNT          697 a         1843 c         1812 g          682 t
ORIGIN      
        1 gagcgccgtc accaagccca gcgcgtacct cgagaacgtg taccaggacg agcagggcaa
       61 ggtgaacttc ttcgagacct cctacacgca gaacggacga gcggtgttcg ggctcgagga
      121 cctcggcgac tacgccgacc ctcgcgacct cgataccgtc gacttcatgc tgatcctcaa
      181 ccggaacgag aacatcatcc cggccgtggc ccggctcacc cgcacgcagg ctgccgccta
      241 cttcatgctc ggggagacga ccgggacctc ggccggcggg aaggccgagg aggggaagtt
      301 cctccgcgtg ccgggtacga accccttctt ccccctgcgg caggaccagc acgggaaccg
      361 gatcctcgag ctgctcgact cgcaccccga catggaggtg tacctgatga acacggggcg
      421 ggtcgggggc aaggacgagc acgaggggtc caagaaggtc aagatccccc attcctccgc
      481 ggtggtgaag ggcatcgccg agggaacgat ctcgtgggcc gaggacccgg acttcggcta
      541 cgaggtggcc gagagcgttc cggggttcga cgacgaggag ctgctccagc cccgcaggct
      601 ctacgagcgg cagggccgca tggacgagta cgacgcgatg gtcgatcggc tcaagaccga
      661 gcgcgtcgag ttcctgaagc ggttcgaggg actggacgag gagatcatca aggccgtcgg
      721 ctgacggcgg tgggggcgcc cccgcccgag gcgccccgca cccacccctg gctgaggtcc
      781 tcttgttgat cttgttgacg accttgagga tccgaccaac gttctccttc ccgcggccgg
      841 acgagcatcc gcggagaggt gaagggaacc tgaaggtccg ctgtgggcgc cggcgataga
      901 ctcgccgaga tgacggtcga acccgcctcc cgcaccgagg gcggcgcctc caagctccgg
      961 gtcgccgtct gggtcctgca cgtggccctc cccctcctcg ggctctggct gctgctggcc
     1021 cagcccgacc tcgacgtcgc ctgggaggac cacctctggc acttctggct ggtgggcgtc
     1081 gtggcggcga tcaacgcggt cctggggctc cggatcagcg aggccgctcg ccgccacgac
     1141 gacgcgcggc tcttcgtcgt cggcctggcg ttcctggcca gcgcgggctt cttcggcctg
     1201 cacgccctgg ccacgcccac cgtcctgctg accggggcca acgcggggtt ccaggtggcc
     1261 tcgcccgtcg gcctggtcgt cttcgccgcg ctggccgcgg tctccgcgct cgatctgtcc
     1321 gggccgacgg gcgaggcgat catgcggcgc cagcccctcc tgcagtgggc ggtgatcggc
     1381 ctcctcgggg cctgggccgc cgtctcgctg gccggcctgc cgcctctcga cgcggccctc
     1441 gacgccgagc aagcccgcgg gcccgccgcc ctgctcacga tcgccggcgt cccgctgtac
     1501 ggcttcgccg ccggccgcta cgtcgccctg taccggcggc ggccgagcgt gatgctgatc
     1561 agcctcatca ccgcgttcgt gctgctggcc gaggcgctgg ccgcgatgcc gctggcacga
     1621 gcgtgggcgg cctcgtggtg ggcgtggcac gtgctgatgc tggccgcgtt cggcttcatc
     1681 gcctacagcg cgcacgtcca gtaccggcgg gagggcggcg ggacggccct cttcaacgcg
     1741 atctcgctcc gggagaccgt ccggaggatc cgggaggagc acgcgcaggc cctcgaggag
     1801 ctcgtggagg cgatgcgggc gcaggccgag acgggggccc ccgacgccgt gcaccccacc
     1861 gccgcgcggc tcgccgaccg cttcgggctc accgaggggc aggtcgaggt gctggagcgc
     1921 gcggccgagg cgctggccgg ggagcgcgag cagaccgcga ggctggccgc gctcgtggcc
     1981 ctggggcggg agtcacgggt catgctcgac gaggaggccc tgctgggccg ggccctggac
     2041 ctcagcggcg gcgtcttccg agccgaccgc ctcgggatcc gcctcgtcca gggtggacgt
     2101 cttcgaccgc tgcgcgggga cgggacgccg gcccgggccg ccgccgaggc agcccagcgc
     2161 ctggagacga tcgagggctc cgagggcgag gagcgcgtgc tcgccttccc cctgacggtg
     2221 aaggaccggg ccgccggcgt cctggaggtc cgtcgccggg gtcggggatt ccacgagcgg
     2281 gagcggtcgg tcctcgagtc gctcgccgtc cagctgtcga tcgcgctgga gaacgtccgg
     2341 ctgtacgcgc agatcgacgc cctcttccgc cagtacctct cccccgacgt ggccacggcg
     2401 ctgctcgccg accccgacca ggcctcgctc ggcggcgggc tgaccgaggt caccgtgctg
     2461 ttcggggatc tccgggggtt cacctcgttc tccgagcgga cggaccctgg ggccgtggtc
     2521 gagctgctga acgcgtactt cggggcggcc gtgccgctcg tcctggcccg gggagggacc
     2581 gtggacaagt tcgtgggcga cgccatcatg gcgctgtggg gggcccccac gcgtcagctc
     2641 gatcacgcgc ggcgggccgc cgcggcggcc ctcgcgatcc agggcgcgat cgaggacctg
     2701 cccggcgatc ccgaccggcc gcgcttccgc atcgggatca acacgggcac cgcgctcgtg
     2761 gggaacatcg gcagcgacga gctccgcaac tacacggcga tcggcgacac ggtgaacctg
     2821 gcgtcgcggc tgcaggagct ggccgagccg ggccggatcg cgatcgggga ggcgaccctc
     2881 caggcgctgg gcgagggggc cgtggtcgag cccctggggg aggtccgggt caagggccgg
     2941 gaggcgccgg tccgagccta cctgctggag gatctcctca ccggccccgg ctgagcccga
     3001 ccgcggccag catgcgcagg gccgccatcg agatcgggat catgccgaac cagcggaacg
     3061 cgtagggatc gtccacgagg gcgaagccca cgaagagccc ggccagcgcg agccagaacc
     3121 cggcccgagc cgcgtcgtgg acggcgatca gcaggcggtc ggtccccgag agccggccct
     3181 cgagctgcgt gccgtcgaac cccgctctca cgccgatcag gaagaaggcg ccggccagga
     3241 cccaggccag gacgtccagg gtcgcggtca cgcccgggcc tcgtccagca gccggacgac
     3301 ctcctcgtcg gaggtctgct cgaagtcccg gtaccactgc cccaccgcgt agaagcgctc
     3361 gggggcggcc aggaccacga cccggtcggc ctcccgggac agtcgccgga tcgactcggg
     3421 ggccccgacg ggcaccgcca gcaccacggc ctcggcgccc tgggcccggg cccaccgcag
     3481 ggccgccgcg gccgtacccc cggtggccac gccgtcgtcc acgacgatcg ccgtcttccc
     3541 ggccacggac gcggggggac ggccgcggcg gtaggcctcg gctcgccgcc ggatctcggc
     3601 ctcctggcgc tcgatctccc gatccaggta ctcctgcggc acgcccagcc tggccacgat
     3661 gccctcgtcg agcacccgca cgccgggggc gatcgcgccc agcccgagct cggggttgcc
     3721 cggcgccccg atcttccgcg ggatcaccac gtcgagcggc cacccccggg cccgcgctac
     3781 ctcggccgcc accggcacgc ctccccgcgg gatccccagc accacgacgt cgtcctccag
     3841 cccctccagg gcctccgcca ggaggcgccc ggcctcggtt ctgtcggcga acccccccga
     3901 gggaagcccg aagccctcgt ccatgcgggc attatccccc ggggctccgt ggtgacccgc
     3961 cgggccgagg gcgtagaatg tggcacttcg acggacggga ggtgccacgt ggtaaacgcc
     4021 tacatcctga tccagaccga ggtcgggaag gccgcccagg tcgccaagga ggtcgccgcg
     4081 atcgatggcg tcacctcggc cgaggacgtc accggcccct acgacgtgat cgtgcgcgcc
     4141 gaggccggga acgtcgacga tctcggaaag ctcgtcgtgg cgcggatcca gtccgtggag
     4201 ggcatcaccc ggacgctgac ctgcccggtc gtccacctct gagccgctcc cacctccccc
     4261 cgggggaggg tgcgataatc acacccgtgt gattcgtgtc gtacccgcgt ggcacgatgg
     4321 gacgcggagg tgagagcgta tgcgagagtg tgacggctgc cggagcgaga tgcgcgtggt
     4381 ggactcctac gtggccctgg aaggggcgca ggaggtcgag gtcatccgct ggcggtgccc
     4441 gaggtgcggg gaggagagtg agtcgaccga ccggctcctc gaggtggcct gactaggctt
     4501 ccccccggat ccgctccccc agctccctca ccacgtcggg gatgaggtcc acgtcctcct
     4561 cggacgtccg ccagttcacg atcgcgggcc ggaaggccgc cttcccctcg taggtcgtcg
     4621 tccccacgta gaggcgcccg tccgccatcg cctcccggcc gatgcgctcg ttcagctcgt
     4681 ccaggtcctc ctcggggacg cccggtgggc ggtaccggaa gcagacgata ttgagctgcg
     4741 cctcggccag cagctcgagg tcgggagcct cctcgacctt cctcgccacc cgctgggcca
     4801 ggtccagatg tcgctcgacg atcccgcggt agccctcgcg cccgtaggcc ctgagggtgg
     4861 cccagacggt gaagccgcga gcccggcgcg acgactccgg cccgaggttg aagtacacgg
     4921 tgcgctcctc gtccggcggc gggaggtacg cggcggccac ggagaagacc cgtcccagcc
     4981 gggagggctc gcgcaggaac gcgaacccgc agtcgtgcgg gacgttcagc cact
//
LOCUS       DASUTW010000180        19060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1740476, whole genome shotgun sequence.
ACCESSION   DASUTW010000180 DASUTW010000000
VERSION     DASUTW010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 19060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19060
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740476"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /gene="fdxA"
                     /locus_tag="VF058_06240"
     CDS             complement(<1..207)
                     /gene="fdxA"
                     /locus_tag="VF058_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX6207944.1"
                     /translation="MAYVITEPCIDVKDRACVDECPVDCIYEGGRMLYIQPDECVDCD
                     ACEPVCPVTAIFAEDDVPEEWKNFT"
     gene            complement(498..1460)
                     /locus_tag="VF058_06245"
     CDS             complement(498..1460)
                     /locus_tag="VF058_06245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6207945.1"
                     /translation="MDGLAVALNGYGLRGEDGRREVLAWHDVVQIARTAEEAGYQAIF
                     APEIGGREAFSMLVGLAAETSGIRLVTGVAPIGSRDPRRMAMEAATLHDLSNGRGVLG
                     LGSREPIDRTREEIAAIRALLAGDEPKIEGDGAAAIPGLDLFPGRMPIYLAALGPRMT
                     ELAGEVADGVILNWCTPERVAQAHEQLARGAARAHRDVSTVTVCVYVRAAVGMSEELA
                     LEALRAALAEYAGMPTYARQLEAMGLGEEGRRAAEATSVDEVPESMVDALCVRGGRDE
                     ALARLGEYYDAGADVVVVYPVPELDAATSITGTVLGLAPSPALG"
     gene            1483..2127
                     /locus_tag="VF058_06250"
     CDS             1483..2127
                     /locus_tag="VF058_06250"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6207946.1"
                     /translation="MTTTTFQTDDGVTLEGELRDPEGEARGTAVICHPHPAHGGSKDH
                     PLLWAIRNELARRGWVVLAFNFRGVMGSGGEHSGGEEETRDLRAAIDRVREEAEGPTV
                     VVGWSFGANVAVRHSITDERVAAVALVGLPIGFDLLPVIELPDNEDLRAWETPVLLVA
                     GDDDAICPVPGMLELSGRIPNADVEVIEGGGHFFRRMEREPATAIAEFFDEVLQ"
     gene            complement(2121..2324)
                     /locus_tag="VF058_06255"
     CDS             complement(2121..2324)
                     /locus_tag="VF058_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207947.1"
                     /translation="MALLIIIILIIAAVTGTLWQVLEIAAGVAIGIFLATVLLAVAAY
                     YAVRSRLRRFQRDMRRDDRPERY"
     gene            2395..3150
                     /locus_tag="VF058_06260"
     CDS             2395..3150
                     /locus_tag="VF058_06260"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX6207948.1"
                     /translation="MEVLIGTSGWQYDDWRGVLYPEGVAKKDWLVHYSERFPVVEVNN
                     TFYNLPADDTFVRWRRGSAEGFRFVVKASRFITHIRRLRECRDPVQLLWSRAERLGRK
                     LGPVLFQMPPNLRADHERLRAFLEILPKHMEAAFEFRHRSWENDDTYDLLDRGGYAYV
                     LADRPGARVPGVVTGGWSYLRFHQGTENGPGYTRRKLRRWADRIAGLDARTVYVFFNN
                     DRAGAAVRDTRTLTRLLEDRHDLRVKGPVRGAA"
     gene            3306..4142
                     /locus_tag="VF058_06265"
     CDS             3306..4142
                     /locus_tag="VF058_06265"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6207949.1"
                     /translation="MHRRRGRPPRRRRDPRGGPGLRRGRALGRDVRGRHPASGAGPHV
                     RRERTGSGARARRRDRGRRAPHRARLHDRSVREHEGRGASREPHPGAGDFLSAYDETK
                     YLAHRLAERRAESGAPVTIAAPGAVYGPEDHSEIGNQLDQLRAGKLRFVAFPETGFNA
                     LHVEDAADGILRVLERGEVGATYPLGGEITTLGDILETAARILGRRLPPRLPSALVKA
                     GIPFGRLIGPAMGVPPNIREVVRAADGVTYWASDARAREELGYAPRGLETGLRETLTG
                     AP"
     gene            complement(4126..5553)
                     /locus_tag="VF058_06270"
     CDS             complement(4126..5553)
                     /locus_tag="VF058_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016188319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6207950.1"
                     /translation="MSETRPFFLAGEARTRDETFEVKSPYDGSVVAEIARPTDEDVEE
                     AVARAADAFEETRALPTHVRAEALMHVSRRIAERADEAAEIIAREGGKPLKWASVEAK
                     RAVSTFRWAAEEIRQGEGELRRLDADAGMEGRLGVMRRFPYGPVLGITPFNFPVNLVA
                     HKVAPAIGVGAPIVIKPATQTPIGALFLAELVAETDLPGGILSVLPISGSKAEPLVTD
                     PRIKKVSFTGSGPVGWRLKGLDPRKRFTLELGGNAGVIVHSDADLDHAAERIAFGGYY
                     QAGQSCIAVQRVLVQSEVYDDFVERFVKQVQALKVGDPLDPSTDVGPVIDAGALDRIA
                     EWVDEAVKGGAEVLAGGERDDPFYRPTILDQTTPEMKVRCEEIFGPVTTVTSYETWED
                     ALAEVNNSEYGLQAGVFTSSLDRAFEAHRELQVGGVVVNDQSAFRADQMPYGGSKESG
                     YGREGLRYAMEDMTEPRIMVLKVPL"
     gene            complement(5570..7015)
                     /locus_tag="VF058_06275"
     CDS             complement(5570..7015)
                     /locus_tag="VF058_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HEX6207951.1"
                     /translation="MADHKKMFIGGEWVDGSGESVSVINPATGAVIAETPNATEADVD
                     RAVQTAKRTFEEIWFDSTPGERQAMMLSWADAIEANAEELGRIESENVGKPISWVVSD
                     EIMVVAEQMRFFAGAARVMGGLSAGEYYKEDTTLFTSWLRREPLGVVGLIAPWNYPLY
                     MAMWKFAPALAAGNTIVIKPSEWTPLTLLRAAELAADVFPPGVINVVTGEGEPTGASI
                     VRHPDVAMVSLTGEVTTGKIIAREASETLKRVHLELGGKAPVLVFDDADLESSVEWIK
                     TAGYFNSGQDCTAATRVIAGPKIYDDLISNLVSGVEGMKVGDPSEEDTEMGPVVAEQQ
                     LERVTGFVDRAREQGASVLTGGERIGGEGFFYRPTVVADVGQDFEIVKNEVFGPVVTV
                     QRFEDDDQAIAWANDVDYGLAASVWTRDVGRALNASRRLRFGTVWINTHIPLTPEMPH
                     GGFKQSGYGKDMSVYSLEHYTELKHVMAAID"
     gene            7162..8301
                     /locus_tag="VF058_06280"
     CDS             7162..8301
                     /locus_tag="VF058_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207952.1"
                     /translation="MAVCLRCEWEGPLPAPERCPSCGVPLFEAGMRPATRSRSRTTRS
                     GTAVLEAEVVEQRHEPEGPARATRRRWALPLALVLVAAAILLLPRQDPPRPARPGVAN
                     PPPQTLRFIALPPGLSDRTVNRPVRIAFSATQPVPEDRAQLFQLWRLDLPTGELTPGP
                     IVGDVLLIRYAPDDSGRLAFLVSGGGLFVLDGFLASRPTWVDGGVSAFDFAPGGALVY
                     AKVERRSAPGGGAPAAVRLSTVEPGRTTPSRVRSRTIHRLNLRGYAVRGWELIGWGVR
                     EGQEQAIAWNVRDGRVRRAPEFRLVPRARSRAGSWELGARGLRRISRAIPRDPAHLAV
                     SGDLAFWAGDDGLAVAERDGGGSFVVELPSRFPIPTGPVAATRGR"
     gene            complement(8397..9305)
                     /locus_tag="VF058_06285"
     CDS             complement(8397..9305)
                     /locus_tag="VF058_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03620 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6207953.1"
                     /translation="METDGRAAARALGRVGVWTFALDANPVAAAREAVAELDELGYGA
                     VWIPEGRGSKEVLSHAAVLLGASDRILVATGIANIWFRDPAAMASGARTLADGFPGRF
                     VLGIGVSHAPTVERRVGLAYERPYSRMVAYLEGMEAARYPAGGAGGAPPLVLAALGPR
                     MLRLAAGRAAGAHPYFVPVEHTARAREILGEAPLLAPEQAVVLEADPGRAREVARGYM
                     GHYLKLPNYAANLRRMGWSDDDIDGPSDALVDAIVAWGDEGAVVRRVREHLDAGADHV
                     SVQVLLEDARAIPMDGYRRLAPALLD"
     gene            complement(9316..10143)
                     /locus_tag="VF058_06290"
     CDS             complement(9316..10143)
                     /locus_tag="VF058_06290"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020395056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6207954.1"
                     /translation="MGRGWGLRVASGAVILFLYFPITIIFLYAFTTEAAAFTFPPPAL
                     TTRWFGVAADNPAIWEALRLSLTVAAITTAIALVFGSLMAAAVYRTRFFGREAISFLV
                     VLPIALPGIVTGIALRSSIAILDVPFSLWTIVIGHATFTVVIVYNNVLARFRRMSPSL
                     VEASMDLGASGWQTFRHILLPNLGTALLAGGLLAFALSFDEIIVTTFTAGQQQTLPIW
                     IFSSLFRPRARPVTNVVAIMVVLVTAIPILLAARLARGESGGRIGARPTAGPGGGAQ"
     gene            complement(10143..11075)
                     /locus_tag="VF058_06295"
     CDS             complement(10143..11075)
                     /locus_tag="VF058_06295"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017788200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6207955.1"
                     /translation="MAVATETIPIARRVSAFLYRHPGIKLVLLLSPGLALLGVIYGGS
                     LAALLLQSLYRLEEFTGLIVRDVSLDTLGQLTDRAHLDIIARTTAMAAAVTIGCAVVA
                     FPLAYYMAKFASPRMKTLLYLLVLMPLWSNYLVRVISWKLILAQEGIFNWFVDKLALD
                     GVLRWALGAPMIGGPSISQSFLGMWIVFVYTWLPFMILPLSAALERVPDSLTDASGDL
                     GARPPMTFRKVILPLALPGLIAGSIFTFSLTLGDFIIPSVIGNSTFFIGPFVLLQQGT
                     AGNLPLAAAMSLVPLAIMTLYLLGARRAGAFEAL"
     gene            complement(11152..12381)
                     /locus_tag="VF058_06300"
     CDS             complement(11152..12381)
                     /locus_tag="VF058_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6207956.1"
                     /translation="MRAHLRWWALLAVVVLAAASCGDDGGEGGEAAGGGGGGMITELG
                     ENEGQVSIIAWAGYIEDGSTDPAYDWVSGYQQETGCEVSVKTAATSDEMVALMQEGSG
                     FDLVTASGDASLRLITGGTVQPINLDLIPSYDTVDERLQDAPWHTVDTDGDGEAEHYG
                     APYEWGSNVLMYNTEAFAGETPTSWSAVFEEQTLPDGESNRGRVQAYDGPIYVADAAL
                     YLMHHQPELGITDPYALTREQFDAALELLRQQRELVSRYWHDAFIQIDDFTNEGIAAS
                     SSWPFQVNILKAEGEPIDSVIPEEGATGWADTTMMHVNAPHPNCAYRWLEWSLNPKVQ
                     GDLAAWFGSVPSVPAACEESELLGPDGCQTNGFDNFDQIHFWKTPQSDCFEPGSGKEC
                     VPYHEWVTSYVAVIGGR"
     gene            complement(12378..13481)
                     /locus_tag="VF058_06305"
     CDS             complement(12378..13481)
                     /locus_tag="VF058_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6207957.1"
                     /translation="MRETTSAVEFRDVRKRFGDVVAVDGVDLEIRQGEFFSLLGPSGS
                     GKTTCLRMIAGFELPSEGTVLLSGRDVTRLPPYDRDVNTVFQDYALFPHMTVAENVGY
                     GLMVRKATRDKRRGRVAEALRMVRLEGFDDRKPQELSGGQRQRVALARALVNQPSVLL
                     LDEPLGALDLTLRQQMQIELKAIQESVGLTFIYVTHDQEEALTMSDRMAVLNEGRVEQ
                     VGAPADVYERPTSEFVAGFVGVSNVLHGEAARAITGEAGSFTIRPEKIRIAGLDDRPG
                     PEEHSATGSVRDVVYLGAQTRYFVELDSGEGLVVLRQNLEGTSTDALQIQGRKVRLIW
                     NRQFNRSVEGAEGDAGPAGPGAGPAGPGEEEDA"
     gene            complement(13668..15221)
                     /locus_tag="VF058_06310"
     CDS             complement(13668..15221)
                     /locus_tag="VF058_06310"
                     /inference="COORDINATES: protein motif:HMM:NF019520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEX6207958.1"
                     /translation="MTLRVDDILRSPGLALQLVGGRSGKGNIVRWVHVSELEDPTPWL
                     KGGELLLTTGMGVGRSAAKQRAYVRRLADAELAGLGFGIGFSFTKVPKAIRDAADELG
                     FPVFEVPYAVPFIAITEMVFSRAAGEQVEILQRSIEAQTALTRAVIDGEGVDGVVREL
                     AGVVGGWAVVLELHGVALATAPPSAAGRAARLWEEIQARRADGPGFSLSLVDRGDHVS
                     IQPVGARGHVEAFLAVGKGEGLSQLDRVVAAHAVSLIAIELDRRRAVVDTERRLRGDF
                     LDALVRGELSARKAAEGLHRFGFERTDEVAVLALESPLPVEDLLWAAEDVLSRRGGGY
                     LTAPREQDLYVLVHPDGDEGFGELRRRVAGKLRSSVRMGAGSTVRPTKAGSSLREASY
                     ALKVARMEGRDSADFRSLGTYRLLLSLQEPDALRAFADSVLAPLDRYDAEHGGELVSS
                     LRAFLEHNARWESAAGELFVHRHTLRYRMRKVEELAGRDLGSAHDRMEFWLALRARDL
                     LRETERPAS"
     gene            15374..16717
                     /gene="gabT"
                     /locus_tag="VF058_06315"
     CDS             15374..16717
                     /gene="gabT"
                     /locus_tag="VF058_06315"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEX6207959.1"
                     /translation="MEIPQERRLVTEIPGPRSRELLARRETAVPRGVFNTVPIFVRAA
                     SGGIVEDVDGNRLIDFGTGIAVLNVGNASPPVVEAIREQAGLYTHTCFHVTMNEPYLA
                     LAERLNQLTPGDHHKMTMLVNSGAEAVENAIKISRYATGRPAVVTFDHAFHGRTLLAM
                     SLTGKVMPYKQGFGPFAPDVYRFPISYPYRCATGEPYDECGPRCADLVIDGIEQHIGA
                     ENVACVILEPVQGEGGFVVPGTEFVRRLSAWCAESGALFVADEIQTGFGRTGRWFASE
                     RFEVVPDIVVTAKAMGGGLPIGGVTGPRDAMDAIHVGGLGGTFGGNPVAAAASLAVID
                     QIEREGLLERAERVGATMTEALRGFAERFQVIGDVRGLGAMVAMELVSDRGTKEPAKE
                     AAAQVLHECHTQGLIALKAGTYDNVVRLLPPLTIDDALLEEGLGILEKALAAVSD"
     gene            complement(16731..17585)
                     /locus_tag="VF058_06320"
     CDS             complement(16731..17585)
                     /locus_tag="VF058_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6207960.1"
                     /translation="MSPKAHAVRTGLRRGWTEFVLSVRSPQDQGFYVFSAAIVLLYLF
                     FNRDSDVAGTSLSHAAVTLPSILGAMVTFSLIIGPAYQLAMDREDGTLLRARALPHGI
                     LGYVSGQVLTHSLALIPSFAVILLPSVVLFEGVMQGGPGGWLTMVWVIGLGLLAAMPI
                     GIIIGSLAPGVQKVGTWGMLPVLVLFGISGIFSPLQSLWGWVQVVAQVFPMYWLGLGM
                     RSAFLPDAAAAIEIGGSWRTLETVAVLGAWAVAGLLLAPVVLRRMARRQSGSQVEAAR
                     HEAVQWVR"
     gene            complement(17582..18505)
                     /locus_tag="VF058_06325"
     CDS             complement(17582..18505)
                     /locus_tag="VF058_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017623724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6207961.1"
                     /translation="MTAQEGVIEVAGLRMRYGSVDVLDGVTFSVSRREVVVLLGPNGA
                     GKTTTIEVVEGFRIRSAGDVSVLGVDPAQAGESWRARVGVVLQSWRDHARWTPRALLS
                     HLGRYYEPYSTAERARPHEADTLIEMVGIEAQADRKIRTLSGGERRRLDVAIGLVGRP
                     ELLFLDEPTAGFDPRARKEFHELIHRLSDLEDTTILMTTHDLDEAEKVADRILILARG
                     RIVADGTADELARLVTRDAQVRWSRNGERFTHATPDATRFVRELFDRHGDEVSDLEVR
                     RATLEDIYLEMVQRHEAGVQEAAPGFEGLGR"
     gene            complement(18556..18873)
                     /locus_tag="VF058_06330"
     CDS             complement(18556..18873)
                     /locus_tag="VF058_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207962.1"
                     /translation="MSGAYIQEVRPFTTVNVALPPADQSGGVKYGATWLSLGTDFGTA
                     TVRLAIGRPGDFRIEDNLQVPTGRQVVRRLRGGIVDQVASIVHLGGPPVSVLVEFETR
                     PDF"
BASE COUNT         2802 a         7020 c         6554 g         2684 t
ORIGIN      
        1 ggtgaagttc ttccactctt cgggaacgtc gtcctcggcg aagatggccg tcaccgggca
       61 gaccggctcg cacgcgtcgc agtccacgca ctcgtcgggc tggatgtaga gcatgcggcc
      121 gccctcgtag atgcagtcca cagggcactc gtccacgcac gcccggtcct tgacgtcgat
      181 gcacggctcg gtgatgacgt aggccacgct ctcctcctag ttttcccgat tgccacggtg
      241 ccgaccgtgg gaaccccagt ctaccctcgg agggcgcgaa cctcaacgct cagatccaca
      301 ggaggaggac cgaggccacg agcaggatgg cgaggcccag gggccaccgc gcggcggcgc
      361 ctcctcgaga gcgagccccc ggcggaggtc gcggtgagcg tcggcttcac gaccaggcgc
      421 acctcacccg cccacttcaa gcgccacgtg gggacgacgg cggggcgcac gccgcccggc
      481 gggccgcgta gggccggcta cccgagggcc ggagagggag cgagccccag cacggtgccc
      541 gtgatcgagg tggctgcgtc cagctcgggg acggggtaca cgacgacgac gtccgcgccg
      601 gcgtcgtagt actcgccgag gcgcgccaga gcctcgtccc gccccccgcg cacgcagagc
      661 gcatcgacca tcgactcggg aacctcgtcc acggaggtgg cctccgccgc ccggcgaccc
      721 tcctcgccaa ggcccatcgc ctccagctgg cgggcgtagg tgggcatccc cgcgtactcg
      781 gcgagcgctg ctcggagcgc ctccagcgcg agctcctcgc tcatcccgac cgcggcccgc
      841 acgtacacgc agaccgtgac cgtcgagacg tcccggtggg cccgcgcggc gccgcgagcc
      901 agctgctcgt gggcctgggc cacccgctcg ggcgtgcacc agttgaggat cacgccgtcg
      961 gccacctcgc cggccagctc ggtcatccgc ggcccgagcg cggcgaggta gatcggcatg
     1021 cgaccgggga agaggtcgag tcccgggatc gcggcggcgc cgtcaccctc gatcttcggc
     1081 tcatcccccg ccagcagggc ccggatcgcg gcgatctcct cccgggtcct gtcgatcggc
     1141 tcccgcgacc cgaggccgag gacccctcgg ccgttcgaca ggtcgtggag cgtggcggcc
     1201 tccatcgcca tccgcctcgg gtcgcgggag ccgatcggcg ccacccccgt cacgagacgg
     1261 atgccggacg tctccgcggc gagccccacc agcatggaga acgcctccct ccccccgatc
     1321 tccggcgcga agatcgcctg gtagccggcc tcctcggccg tcctcgcgat ctgcacgacg
     1381 tcgtgccagg cgagcacctc ccggcgcccg tcctcccccc ggaggccgta gccgttcagc
     1441 gccaccgcga gcccgtccat cgtccccgta tcatccttca tcgtgaccac caccaccttc
     1501 cagaccgacg acggggtgac cctcgagggg gagctccgcg acccggaggg agaggcacgc
     1561 ggcacggccg tcatctgcca cccccacccc gctcacgggg gcagcaagga ccaccctctg
     1621 ctctgggcga tccggaacga gctggcccgg cggggctggg tcgtcctcgc cttcaacttc
     1681 cggggcgtga tgggctccgg gggcgagcac agcggaggcg aggaggagac gagggacctg
     1741 cgggcggcga tcgaccgggt tcgggaggag gcggagggcc ccaccgtggt cgtcggctgg
     1801 tccttcggcg cgaacgtggc cgtccgtcac tcgatcaccg acgagcgcgt ggcggccgtg
     1861 gccctcgtcg gcctgccgat cgggttcgac ctgctgccgg tcatcgagct ccccgacaac
     1921 gaggacctcc gcgcgtggga gacgcccgtc ctgctcgtgg ccggggacga cgacgcgatc
     1981 tgtcccgtgc cggggatgct ggagctctcc ggccgcatcc cgaacgcgga cgtggaggtg
     2041 atcgagggcg gaggccactt cttccgcagg atggagcggg agcccgcgac ggcgatcgcc
     2101 gagttcttcg acgaggtgct tcagtagcgc tcggggcggt cgtcccgccg catgtcgcgc
     2161 tggaagcgcc gcaggcggct ccgcaccgcg tagtacgcgg ccaccgcgag cagcacggtc
     2221 gccaggaaga tcccgatggc gacgccggcc gcgatctcca gcacctgcca cagggtgccc
     2281 gtgaccgccg cgatgatcag gatgatgatg atcagcagtg ccacgccccc atccttcccc
     2341 aagttccggc gtctcgcacg ttgccgatga cgggaaacgg ggcaaggtga cgacatggaa
     2401 gtcctgatcg gcacgagcgg ctggcagtac gacgactggc gcggcgtgct gtatccggag
     2461 ggggtggcca agaaggactg gctggtccac tactcggagc gattccccgt ggtcgaggtc
     2521 aacaacacct tctacaacct gccggcggac gacaccttcg tgaggtggcg tcgcgggtcc
     2581 gccgaggggt tccggttcgt ggtgaaggcg agcaggttca tcacccacat ccgccggctt
     2641 cgcgagtgcc gggacccggt gcagctcctg tggtcgaggg ccgagcggct ggggcggaag
     2701 ctgggccccg tcctgttcca gatgcccccg aaccttaggg cggaccacga gcggctccgg
     2761 gccttcctcg agatcctccc caagcacatg gaggcggcgt tcgagttccg ccatcgctcg
     2821 tgggagaacg acgacacgta cgacctcctg gaccggggcg ggtatgcgta cgtgctcgcc
     2881 gaccgacccg gcgcgcgggt gcccggcgtc gtgaccggcg gatggtccta cctccgattc
     2941 caccagggga ccgagaacgg gcccggctac acgaggcgaa agctccgccg gtgggccgac
     3001 cggatcgccg gcctcgacgc ccgcaccgtc tacgtcttct tcaacaacga ccgggccggc
     3061 gcggcggtgc gagacacccg cacgctgacc cggctgctcg aagatcggca cgacctccgg
     3121 gtgaagggcc ccgtgcgcgg ggcggcctga cgctcccctc ggtacgctct cggcgtgaag
     3181 gtcttcctca ccggcggcac cgggttcgtg ggcggcgagg tcgctcggcg gctgcgggct
     3241 cggggcgacg aggtcgttgc gctcatccgc acacggccga aggccgcctc gctcaccgac
     3301 ctcggatgca ccgtcgtcga gggcgacctc cacgccgtcg acgcgatccg cgaggcggcc
     3361 cggggctgcg acggggtcgt gcactcggcc gggatgtacg aggtaggcat cccgcgagcg
     3421 gagcgggccc gcatgttcga cgcgaacgta cggggagcgg agcgcgtgct cgacgccgcg
     3481 atcgaggccg gcgtgccccg catcgtgcac gtctccacga tcggagtgtt cgggaacacg
     3541 aggggcgagg tgcatcccgg gagccccacc ccggagcggg tgacttcctc agcgcctacg
     3601 acgagacgaa gtacctcgcc caccgcctgg cggaacggcg tgccgagagc ggcgcgccgg
     3661 tcacgatcgc cgctcccgga gccgtgtacg ggccggagga ccactccgag atcgggaacc
     3721 agctcgatca gctccgcgcg gggaagctcc ggttcgtggc ctttcccgaa accgggttca
     3781 acgcgctcca cgtggaggac gcggccgacg ggatcctccg cgtgctggag cgaggcgagg
     3841 tcggtgccac ctaccctctg ggcggcgaga tcacgacgct cggcgacatc ctggagacgg
     3901 cggcgcggat cctcggccgg aggctccccc cacggctccc ctcggcgctg gtcaaggcgg
     3961 ggatcccctt cgggcgcctg atcgggcccg ccatgggcgt gcctcccaac atccgagagg
     4021 tggtccgggc ggccgacggg gtcacctact gggcgagcga cgcccgggcc cgcgaggagc
     4081 tgggctacgc cccccggggc ctggagaccg gcctgcgcga gaccctcaca ggggcacctt
     4141 gaggaccatg atccggggct cggtcatgtc ctccatcgcg tagcggagcc cctctcggcc
     4201 gtagccggat tccttgctcc cgccgtaggg catctggtcg gcccggaacg ccgactggtc
     4261 gttgacgacg accccgccca cttggagctc ccggtgggcc tcgaaggccc ggtccagcga
     4321 cgacgtgaac acgccggcct gcaggccgta ctccgagttg ttgacctcgg ccagcgcgtc
     4381 ctcccaggtc tcgtaggacg tgaccgtcgt caccggcccg aagatctcct cgcaccgaac
     4441 cttcatctcg ggcgtcgtct ggtccaggat cgtcggccgg tagaacgggt cgtcccgctc
     4501 gccgccggcc aggacctcgg cgccgccctt gacggcctcg tccacccact cggcgatccg
     4561 gtccagcgct cccgcgtcga tcaccggccc gacgtcggtg gaggggtcga gcgggtcccc
     4621 caccttcagc gcctggacct gcttcacgaa gcgctccacg aagtcgtcgt agacctcgga
     4681 ctggacgagc acgcgctgga cggcgatgca cgactgcccg gcctggtagt agccgccgaa
     4741 ggcgatccgc tcggccgcat gatcgaggtc ggcgtccgag tggacgatca cccccgcgtt
     4801 gcccccgagc tccagcgtga accgcttccg ggggtcgagg cccttcaatc gccatcccac
     4861 gggccccgag ccggtgaagg agaccttctt gatccgcggg tcggtgacga gcggctccgc
     4921 cttcgatccg gagatcggaa ggaccgagag gatcccgccc ggcaggtccg tctccgcaac
     4981 cagctcagcc aagaacagcg ccccgatcgg cgtctgcgtc gcgggcttga tcacgatcgg
     5041 cgcccccacc ccgatcgccg gcgccacctt gtgcgccacg aggttcaccg ggaagttgaa
     5101 gggcgtgatg cccagcaccg gcccgtaggg gaaccggcgc atcacgccga gccggccctc
     5161 catccccgcg tcggcgtcga gccgccgcag ctcgccctcg ccctgacgga tctcctcggc
     5221 cgcccagcgg aaggtggaca cggcccgctt cgcctcgacc gaggcccact tcagtggctt
     5281 cccaccctcc ctggcgatga tctccgcggc ctcgtcggcc cgctccgcga tgcgcctcga
     5341 gacgtgcatc agcgcttcgg cgcggacgtg cgtggggagg gcgcgcgtct cctcgaacgc
     5401 gtcggcggcc ctcgccaccg cctcctcgac gtcctcgtcc gtgggacggg cgatctcggc
     5461 cacgaccgag ccgtcgtagg ggctcttcac ctcgaacgtc tcgtctcggg tgcgggcctc
     5521 gcccgccagg aagaacggcc tggtctcgct cactgctggg tcacctcgct cagtcgatcg
     5581 ccgccatcac gtgcttcagc tccgtgtagt gctcgaggga gtagacgctc atgtccttcc
     5641 cgtaccccga ctgcttgaag ccgccgtgcg gcatctccgg ggtgagcggg atgtgcgtgt
     5701 tgatccacac cgtcccgaag cggagcctcc tcgatgcgtt cagggctcgt cccacgtcgc
     5761 gggtccagac cgaggcggcg agcccgtagt ccacgtcgtt ggcccacgcg atcgcctggt
     5821 cgtcgtcctc gaaccgctgg acggtcacca cggggccgaa gacctcgttc ttgacgatct
     5881 cgaagtcctg ccccacgtcc gccaccacgg tgggtcggta gaagaagccc tcgccgccga
     5941 tccgttcgcc gccggtgagg accgaggcgc cctgctcccg ggcgcggtcc acgaagcccg
     6001 tcactctctc cagctgctgc tcggccacga ccgggcccat ctccgtgtcc tcctcggagg
     6061 gatcgcccac cttcatgccc tcgacccccg agacgaggtt cgagatgaga tcgtcgtaga
     6121 tcttcggacc ggcgatcacg cgtgtggcgg ccgtgcagtc ctgccccgag ttgaagtagc
     6181 ccgcggtctt gatccattcg accgacgact cgaggtccgc atcgtcgaag accagaaccg
     6241 gcgccttgcc gcccagctcg aggtggacgc gcttcagcgt ctcggaggcc tcgcgggcga
     6301 tgatcttgcc ggtcgtgacc tcgccggtca gcgagaccat ggccacgtcg gggtggcgca
     6361 cgatcgaggc gccggtcggc tccccctcgc cggtgacgac gttgatcacg cccggcggga
     6421 agacgtcggc cgcgagctcg gcggcccgca gcagggtcag cggcgtccac tcgctcggct
     6481 tgatgacgat cgtgttcccc gcggcgagag ccggggcgaa cttccacatc gccatgtaca
     6541 gcgggtagtt ccagggggcg atcaggccca cgaccccgag aggctcacgc cggagccagc
     6601 tcgtgaagag ggtcgtgtcc tccttgtagt actcgcccgc cgacagcccg cccatcacgc
     6661 gggccgcgcc ggcgaagaag cgcatctgct cggccaccac catgatctcg tcggacacca
     6721 cccacgagat cggcttgccc acgttctcgg actcgatccg ccccagctcc tcggcgttgg
     6781 cttcgatcgc gtcggcccag gacagcatca tggcctggcg ctcgcccggg gtggagtcga
     6841 accagatctc ctcgaacgtg cgcttggccg tctggaccgc gcggtccacg tccgcctcgg
     6901 tggcgttggg ggtctcggcg atgaccgctc ccgtggccgg gttgatcacc gagacgctct
     6961 cgccgctgcc gtcaacccac tcgccgccga tgaacatctt cttgtggtcg gccatgaccc
     7021 ctccttggga agcctgcggg ttcccagcct atcgggagcg gggggagcgc gtgctcgacg
     7081 cggaggccaa ggcgaccacc cgcgatgtac ggcacggata aggccgtcat cttcccaggt
     7141 gagcaggggt atcctcgctc cgtggccgtc tgtctccgat gcgagtggga gggccccctc
     7201 cccgcgccgg aacgctgccc ctcctgcggc gtcccgctct tcgaggcggg gatgcggccg
     7261 gcgacgcgct cccgctcgcg gacgacccga tcggggaccg ccgtcctgga agcggaggtg
     7321 gtcgagcagc ggcacgagcc ggaagggccc gctcgggcca cgcgcaggcg ctgggccctc
     7381 cccctggcgc tcgtactcgt ggcggcggcc atcctcctgc tgccccgcca ggaccccccg
     7441 cgcccggcgc ggcccggcgt ggccaacccc ccaccccaga cccttcggtt catcgcgctg
     7501 ccccccggac tctcggaccg caccgtgaac cgacctgtgc gcatcgcctt cagcgcgacg
     7561 cagccggtgc ccgaggaccg agcgcagctg ttccagctgt ggcggctcga cctcccgacc
     7621 ggcgagctca cgcccggacc gatcgtgggc gacgtcctgc tgatccgcta cgcgccggac
     7681 gactcgggga gactcgcgtt cctcgtgagc ggcggggggc tgttcgtgct cgacgggttc
     7741 ctcgcctccc gcccgacctg ggtcgacgga ggggtcagcg cgttcgactt cgcacccggg
     7801 ggcgccctcg tctacgccaa ggtggagcgt cgttccgcgc ccggcggcgg cgcgcccgcg
     7861 gccgtgcgcc tgagcacggt cgagcccgga cggaccacac cgagcagggt ccggagccgg
     7921 acgatccatc ggctcaacct cagggggtac gccgtccggg gctgggagct gatcggctgg
     7981 ggcgttcggg aagggcagga gcaggcgatc gcgtggaacg tgcgggatgg gcgcgtccgc
     8041 cgcgcccccg agttccggct cgtcccgagg gcccggagcc gcgccggctc ctgggagctc
     8101 ggcgcccggg gtctccgcag gatctcacgc gccatccccc gtgaccccgc ccatctggcg
     8161 gtgtcggggg acctggcgtt ctgggccggc gacgacggac tcgcggtggc ggagcgggac
     8221 ggaggtggga gcttcgtcgt ggagctcccg tcgcggttcc cgatcccgac cgggccggtc
     8281 gccgccaccc gcgggcggtg agctccgacc gcctcgtcga cgcgaacgtc gcggagagca
     8341 tccgggaccg gcgcgtgatc cgggcgttcc gagacgtgga ccggcggggc ggaggatcag
     8401 tcgaggagcg cgggggcgag gcggcgatac ccgtccatcg ggatcgcgcg cgcgtcctcg
     8461 aggaggacct ggaccgagac gtgatcggcg cccgcgtcga ggtgctcgcg gacccgccgg
     8521 acgaccgccc cctcgtcgcc ccaggccacg atggcgtcca cgagcgcgtc ggaggggccg
     8581 tcgatgtcgt cgtccgacca tcccatccgg cggaggttcg ccgcgtagtt cggcagcttg
     8641 aggtagtggc ccatgtagcc gcgggccacc tcgcgggccc ggccgggatc ggcctccagc
     8701 acgaccgcct gctccggcgc gaggaggggt gcctccccga ggatctcccg ggcccgggcc
     8761 gtatgctcca ccggcacgaa gtaggggtgg gcgccggcgg cgcgccccgc ggccaggcgc
     8821 agcatgcgag ggccgagcgc cgcgagcacg agcggaggcg cgccgccggc gccccccgcc
     8881 gggtaccggg cggcctccat cccttcgaga tacgcgacca tccgagagta cggccgctcg
     8941 taggccagcc ccacccgccg ctccacggtg ggcgcgtggc tgacgccgat cccgagcacg
     9001 aagcggccgg ggaacccgtc ggccagcgtc cgagcgccgc tcgccatggc cgccgggtcc
     9061 cggaaccaga tgttcgcgat ccccgtggcg accaggatgc ggtcggaggc gccgaggagg
     9121 accgcggcgt ggctcaggac ctccttgctc cccctgccct cggggatcca cacggcgccg
     9181 tacccgagct cgtccagctc ggccacggcc tcgcgcgccg ccgcgacggg gttggcgtcc
     9241 agcgcgaagg tccagacgcc cacgcgcccc agcgcacgcg cggcggccct gccatcggtc
     9301 tccatctcga ccagcctact gcgcgccgcc gccgggaccg gcggtcggac gcgcgccgat
     9361 ccggccaccc gactcgcccc gcgccagccg agcggccagc aggatgggga tcgccgtcac
     9421 cagcacgacc atgatcgcca ccacgttcgt cacgggccgg gcccggggac ggaagaggct
     9481 ggagaagatc cagatcggca gcgtctgctg ctgccccgcc gtgaacgtcg tcacgatgat
     9541 ctcgtcgaag gagagcgcga atgccaggag gccccccgcc agcagcgccg tgccgaggtt
     9601 cggaagcagg atgtgtcgga acgtctgcca ccccgacgcc cccaggtcca tcgaggcctc
     9661 cacgaggctg ggcgacatcc tccggaaccg cgccagcacg ttgttgtaga cgatcacgac
     9721 cgtgaacgtg gcgtggccga tcacgatcgt ccagaggctg aacgggacgt cgaggatagc
     9781 gatcgacgat cggagtgcga tccccgtcac gatcccgggc agcgcgatcg gcagcaccac
     9841 caggaacgag atcgcctcgc gcccgaagaa ccgcgtcctg tagacggccg ccgccatcag
     9901 gctcccgaac acgagggcga tcgccgtcgt gatcgccgcc accgtcagcg agagacggag
     9961 tgcctcccag atcgccgggt tgtcggcggc cacgccgaac catctcgtgg tcagcgcggg
    10021 aggcgggaac gtgaacgcgg ccgcctcggt cgtgaaggca tagaggaaga tgatggtgat
    10081 cgggaagtag aggaagagga tcacggcccc cgacgcgacc cgaagccccc agccccgccc
    10141 catcacagcg cctcgaaggc cccggcccgc ctcgcgccga ggaggtagag cgtcatgatc
    10201 gcgagcggca cgagcgacat cgcggccgcc aggggcaggt tgccggccgt gccctgctgc
    10261 agcagcacga acggcccgat gaagaaggtc gagttcccga tcacgctggg gatgatgaag
    10321 tcgccgagcg tgagcgagaa ggtgaagatc gaccccgcga tcagccccgg cagggccagc
    10381 gggaggatca ccttgcggaa cgtcatcggg gggcgcgccc cgagatcccc ggacgcgtcg
    10441 gtcagcgagt cgggcacccg ctccagggcg gcgctgagcg gcaggatcat gaacggcagc
    10501 cacgtgtaga cgaagacgat ccacatcccg aggaaggact gcgagatcga gggcccgccg
    10561 atcatcgggg ccccgagcgc ccagcgcaac accccgtcca gggccagctt gtcgacgaac
    10621 cagttgaaga tgccctcctg ggccaggatc agcttccacg agatcactcg gaccaggtag
    10681 ttcgaccaca gcggcatcag gacgaggagg tacaggaggg tcttcatccg cggcgaggcg
    10741 aacttcgcca tgtagtaggc gagcgggaac gccaccaccg cgcacccgat cgtgaccgcg
    10801 gccgccatcg cggtggtgcg ggcgatgatg tcgaggtggg cccggtcggt cagctggccc
    10861 agcgtgtcca ggctgacgtc gcggacgatg aggccggtga actcctccag ccggtagagg
    10921 ctctgaagga ggagggccgc cagcgagccc ccgtagatca ccccgaggag ggcgagcccg
    10981 ggagagagca gcagcacgag cttgatgccc gggtgccggt acaggaaggc cgacacgcgc
    11041 cgggcgatcg ggatcgtctc ggtcgctacc gccatggtcc tccgctcgag ggccgggtgg
    11101 gcgaggggcg gggccccgcg cggagccccg ccccccgatc gtcgccgcgg atcagcgtcc
    11161 gccgatcacc gcgacgtagc tcgtgaccca ttcgtggtac ggcacgcact ccttcccgct
    11221 cccgggctcg aagcagtcgg actgcggggt cttccagaag tggatctggt cgaagttgtc
    11281 gaagccgttc gtctggcacc cgtcggggcc gagaagctcg ctctcctcgc atgccgcggg
    11341 gaccgacggc accgatccga accaggcggc caggtcgccc tgcaccttcg ggttcagcga
    11401 ccactcgagc cagcggtacg cgcagttcgg gtgcggcgcg ttcacgtgca tcatcgtggt
    11461 gtcggcccag ccggtcgcgc cctcctcggg gatgacgctg tcgatcggct cgccctcggc
    11521 cttcaggatg ttcacctgga agggccagga ggagctcgcg gcgatcccct cgttcgtgaa
    11581 gtcgtcgatc tggatgaacg cgtcgtgcca gtaccgcgag acgagctccc gctgctgccg
    11641 cagcagctcg agcgcggcgt cgaactgctc cctcgtcagc gcgtaggggt cggtgatgcc
    11701 gagctcgggc tggtggtgca tcaggtacag cgcggcgtcg gccacgtaga tcgggccgtc
    11761 gtaggcctgg accctccccc ggttcgactc accgtcgggg agcgtctgct cctcgaacac
    11821 cgcgctccag ctcgtgggcg tctcgcccgc gaaggcctcc gtgttgtaca tgaggacgtt
    11881 cgagccccac tcgtagggcg cgccgtagtg ctcggcctcg ccgtcgccgt ccgtgtccac
    11941 cgtgtgccac ggggcatcct ggagccgctc gtcgaccgtg tcgtagctcg ggatcaggtc
    12001 gaggttgatc ggctggacgg tcccgccggt gatcagccgc aggctcgcgt cgcccgacgc
    12061 cgtgacgagg tcgaagcccg acccctcctg catcaaggcc accatctcgt cggaggtcgc
    12121 ggccgtcttg acgctcacct cgcagccggt ctcctgctgg tagccgctca cccagtcgta
    12181 ggccgggtcc gtgcttccgt cctcgatgta gccagcccag gcgatgatgc tgacctggcc
    12241 ttcgttctcg ccgagctcgg tgatcatccc tccgccgccg ccccctgcgg cctcgccgcc
    12301 ctcgcccccg tcgtcgccgc agctcgcggc cgcgagcacc accacggcca gcagcgccca
    12361 ccatcggaga tgtgccctca tgcgtcctcc tcctctcccg ggccggcggg cccggctccc
    12421 gggcccgccg gcccggcgtc cccctcggcc ccctcgaccg agcgattgaa ctgcctgttc
    12481 cagatgagcc gcaccttcct gccctggatc tgcagggcgt cggtcgaggt gccctcgagg
    12541 ttctgccgca ggacgacgag cccctcgccc gagtccagct cgacgaagta gcgggtctgc
    12601 gccccgaggt agacgacgtc gcgcacgctc cccgtggcgg agtgctcctc gggacccggc
    12661 cggtcgtcga ggcctgcgat ccggatcttc tcgggccgga tcgtgaatga cccggcctcc
    12721 ccggtgatgg cccgggcagc ctcgccgtga aggacgttcg agacgcccac gaaccccgcc
    12781 acgaactcgc tcgtcggtcg ctcgtagacg tcggcgggcg cgcccacctg ctccacccgg
    12841 ccttcgttga ggaccgccat ccggtcgctc atcgtgagcg cctcttcctg gtcgtgcgtg
    12901 acgtagatga acgtgaggcc cacgctctcc tggatcgcct tcagctcgat ctgcatctgc
    12961 tgacggagcg tcaggtccag cgccccgagc ggctcgtcga gcagcaggac cgagggctgg
    13021 ttgacgagcg cgcgggccag ggccacacgc tgccgctgac cccccgagag ctcctggggc
    13081 ttccggtcgt cgaacccctc gagccgcacc atccgaaggg cctcggccac gcgcccccgg
    13141 cgcttgtccc gcgtcgcctt ccggaccatg aggccgtagc ccacgttctc ggccacggtc
    13201 atgtgcggga agagggcgta gtcctggaag acggtgttga cgtcccggtc gtagggaggg
    13261 agacgcgtga cgtctcgccc cgagagcagc accgtgccct cgctcggcag ctcgaacccg
    13321 gcgatcatcc ggagacaggt cgtcttgcct gagcccgacg gtccgagcag cgagaagaac
    13381 tccccctgac ggatctcgag gtcgacgccg tccacggcga cgacgtcgcc gaagcgcttc
    13441 ctgacgtcgc ggaactcgac cgcggaggtc gtctccctca cttgctcggc gacgtccgtc
    13501 cgtccatcgg tcgaacgtcc ggctacgttg ccgccggcca aggacctccc cccctccgca
    13561 ccggacggtt cataccagag ggaggacctg cgaggacgcg cacgagggag caaccgccgc
    13621 cccccggttg tacggatggg ccaagtcacg cgcccggacg ggcgcgatca gctcgcgggc
    13681 cgctccgtct ctctgaggag gtcgcgggcc cggagcgcca gccagaactc catccggtcg
    13741 tgagccgacc ccagatctct cccggccagc tcctccacct tccgcatccg gtatcggagc
    13801 gtgtggcggt ggacgaagag ctcgcccgcc gccgactccc atctggcgtt gtgctccagg
    13861 aaggcgcgga gcgaggagac gagctcgcct ccgtgctcgg cgtcgtagcg gtccagcggg
    13921 gcgagcaccg agtcggcgaa cgcccgcagg gcgtccggct cctggaggct gaggagcagg
    13981 cggtacgtcc cgagcgagcg gaagtccgcg ctgtcccgac cctccatccg ggccaccttc
    14041 agcgcgtagc tcgcctcccg caggctgctc cccgccttgg tcggccgcac ggtgctcccg
    14101 gcccccatcc ggaccgagct ccggagcttc ccggcgaccc ggcgccggag ctcgccgaag
    14161 ccctcgtccc cgtcggggtg gacgagcacg tacaggtcct gctcgcgcgg ggcggtcagg
    14221 tatccgccgc cgcggcgcga cagcacgtcc tccgccgccc acagcagatc ctccaccggg
    14281 agcggggact ccagcgcgag caccgccacc tcgtcggtcc gctcgaaccc gaagcggtgg
    14341 agcccctccg cggccttccg cgccgagagc tccccgcgga ccagcgcgtc gaggaagtcc
    14401 ccccggaggc gccgctccgt gtcgacgacc gctcgtctcc ggtcgagctc gatggcgatc
    14461 agcgacacgg cgtgggccgc caccacccga tcgagctggg acagcccctc gcccttcccc
    14521 acagcaagga aggcctccac gtgaccccgg gccccgaccg gctggatcga gacgtggtcc
    14581 ccccggtcca cgagcgagag gctgaagccc gggccgtcgg cgcggcgggc ctggatctcc
    14641 tcccagaggc gggccgctcg gccggccgcg gacgggggcg ccgtcgccag cgccacgccg
    14701 tggagctcca gcaccacggc ccatcccccc accacgccgg cgagctcgcg caccacgccg
    14761 tccaccccct cgccgtcgat gaccgctcgg gtgagcgccg tctgcgcctc gatcgaccgc
    14821 tggaggatct ccacctgctc cccggccgcc cgcgagaaga ccatctcggt gatcgcgatg
    14881 aaggggacgg cgtagggcac ctcgaagacg gggaagccca gctcgtccgc cgcgtcgcgg
    14941 atcgccttgg ggaccttcgt gaacgagaac cctatgccga agccgaggcc ggccagctcc
    15001 gcgtcggcga gcctcctgac gtaggctcgc tgcttggcgg ccgagcgccc gacccccatc
    15061 ccggtggtca gcaggagctc cccgcccttc agccagggcg tcgggtcctc gagctcgctc
    15121 acgtggaccc agcgcacgat gttgcccttc cccgagcgcc cgcccaccag ctgcagcgcg
    15181 agccccgggg atcgcaggat gtcgtcgacg cgcagggtca cgtggggatt gtacggttcg
    15241 tacaagagca ccgtcgggcc cgcgggtcgt gtacggtccg tacaaagccc ggctggtcgc
    15301 ttgctcgccg cggccgtgga gcggccgcgg cgagcaagcg acactggcgc cgtccgacct
    15361 tcaggagctg tcgatggaga tcccgcagga gcgccgcctc gtcaccgaga tccccggccc
    15421 ccgctcgagg gagctgctgg cccgccgcga gacggccgtc ccccggggcg tgttcaacac
    15481 cgtccccatc ttcgtccgcg cggcgtcggg cgggatcgtg gaggacgtcg acggcaaccg
    15541 gctgatcgac ttcgggacgg gcatcgccgt cctcaacgtg gggaacgcct cgcctcccgt
    15601 cgtggaggcg atccgcgagc aggccggcct ctacacccac acgtgcttcc acgtgacgat
    15661 gaacgagccg tacctcgcgc tggccgagcg cctcaaccag ctcacgcccg gcgaccatca
    15721 caagatgacg atgctcgtga actcgggggc ggaggccgtc gagaacgcga tcaagatctc
    15781 acgctacgcg accggccgcc ccgccgtagt caccttcgac cacgccttcc acggccgcac
    15841 tctgttggcg atgtcgctca cggggaaggt gatgccctac aagcaggggt tcggcccgtt
    15901 cgcgccggac gtgtaccgct tcccgatctc ctacccgtac cggtgcgcca ccggcgagcc
    15961 gtacgacgag tgcgggccgc ggtgcgccga cctcgtgatc gacggcatcg agcagcacat
    16021 cggggccgag aacgtggcct gcgtgatcct ggagcccgtc cagggagagg gaggcttcgt
    16081 cgtccccggc accgagttcg tgcggcgcct cagcgcctgg tgcgccgaga gcggcgcgct
    16141 cttcgtggcg gacgagatcc agaccggctt cggacgtacg ggcaggtggt tcgccagcga
    16201 gcgcttcgag gtcgtcccgg acatcgtcgt gaccgccaag gcgatgggcg gcggcctgcc
    16261 gatcggcggg gtgacgggcc ccagggacgc gatggacgcg atccacgtgg gcgggctcgg
    16321 gggcacgttc ggcggcaacc ccgtcgcggc cgccgcctcg ctcgccgtga tcgaccagat
    16381 cgagcgggag gggctgctgg agcgggccga gcgggtcggc gccacgatga cggaggccct
    16441 ccgcgggttc gcggagcgct tccaggtgat cggggacgtg cgcgggctcg gcgccatggt
    16501 ggcgatggag ctcgtgagcg atcgcgggac gaaggagccc gcgaaggagg ccgccgcgca
    16561 ggtccttcac gagtgccaca cccagggcct gatcgcgctg aaggccggga cctacgacaa
    16621 cgtcgtacgc ctgctcccgc ctctcacgat cgacgacgcg ctcctggagg agggcctggg
    16681 catcctggag aaagccctcg cggcggtgag cgactagggg cccggacgcc tcagcggacc
    16741 cactggaccg cctcgtggcg ggcggcctcg acctgcgagc cggactgcct gcgagccatc
    16801 ctgcgcagca cgaccggcgc caggagcagc cccgccaccg cccatgcacc gagcaccgcg
    16861 accgtctcca gcgtccgcca cgagccaccg atctcgatgg ccgcggcggc gtccgggagg
    16921 aacgcggatc gcatccccag gcccagccag tacatcggga agacctgcgc cacgacctgg
    16981 acccatcccc acaggctctg gagcggggag aagatccccg agatcccgaa cagcacgagg
    17041 accgggagca tcccccacgt gccgaccttc tgcacccctg gggcgagcga cccgatgatg
    17101 atcccgatcg gcatcgcggc cagcagcccg agcccgatca cccagaccat ggtcagccat
    17161 ccacccgggc ccccctgcat caccccctcg aaaagcacga cgctcgggag gaggatcacc
    17221 gcgaacgagg ggatgagggc gagcgagtgc gtcaggacct ggccggagac gtatccgagg
    17281 atcccatgcg gcagggccct ggcccgaagc agcgttccgt cctcgcggtc catcgccagc
    17341 tggtacgcag gcccgatgat gaggctgaac gtgaccatcg ccccgaggat gctgggcagc
    17401 gtcactgccg catgcgacag ggaggtcccc gccacgtcgg agtccctgtt gaagaagaga
    17461 tagagcagga cgatggcggc cgagaagacg tagaagccct gatcctgcgg gctccggacg
    17521 ctgaggacga actccgtcca ccctcggcgg agccccgtgc gaaccgcgtg cgccttggga
    17581 ctcatcggcc gagcccctcg aagcctggcg cagcctcctg gacgccggcc tcgtgacgct
    17641 gcaccatctc caggtagatg tcctccaacg tcgcccgacg cacctcgaga tcggatacct
    17701 cgtcgccgtg ccggtcgaac agctcgcgga cgaagcgcgt cgcatccggc gtcgcgtgcg
    17761 tgaatcgctc gccgtttcgg ctccaccgga cctgcgcgtc acgggtcacg aggcgagcga
    17821 gctcgtcggc ggtcccgtcg gccacgatcc gcccccgagc caggatgagg atgcggtcgg
    17881 ccaccttctc cgcctcatcc aggtcgtggg tggtcatcaa gatcgtcgtg tcctcgagat
    17941 ccgagagcct atggatcagc tcgtggaact ccttgcgcgc ccgggggtcg aagccggccg
    18001 tgggctcgtc gaggaagagc agctcgggcc gtccgaccag cccgatcgcc acgtcgagcc
    18061 ggcgccgttc acccccggag agggtgcgga tcttccggtc cgcctgggcc tcgatcccca
    18121 ccatctcgat caaggtgtcg gcctcgtggg gacgagcccg ctcggcggtc gagtacggct
    18181 cgtagtagcg cccgaggtgt gagaggaggg ctcgcggcgt ccacctcgcg tggtcgcgcc
    18241 acgactgcag cacgacgccg acccgggccc tccagctctc gccggcctga gcgggatcga
    18301 cgcccagcac cgagacgtcc ccggccgatc ggatgcggaa cccctcgacg acctcgatgg
    18361 tcgtggtctt ccccgcgccg ttcggtccca gcaggacaac gacctctctt cggctgacgg
    18421 agaaggtcac gccgtccagg acgtcgaccg acccgtaccg catgcggagg ccggcgacct
    18481 cgatcactcc ttcctgtgcg gtcatgcctg ctccggagca tactcaccga agcgctccgg
    18541 ccggcagcga tccgatcaga agtcgggccg ggtctcgaac tccaccagca ccgagacagg
    18601 ggggccgccc aggtggacga tcgaggcgac ctggtccacg atgccgcctc gcagcctgcg
    18661 cacgacctgt cgaccggtgg gcacctgcag gttgtcctcg atcctgaagt cgccgggccg
    18721 cccgatcgcc agccggacgg tcgccgtccc gaagtccgtg cccagcgaga gccacgtcgc
    18781 gccgtacttc acccctcccg actgatcggc cggaggcagc gcgacgttca cggtggtgaa
    18841 cggtcgaacc tcctggatgt aggcgcctga catccctcct cctccccgtc gaggtttggc
    18901 gtcacttgta gtcgctcagc gagggggagc caaccggtcg ttctccctac agccccggtc
    18961 ccacgcgctg ttgagcggcc tttcaggacg ggcgatactt cccggggcac tcccccagga
    19021 gacgtcgagc gatgacgggt gacagatgcc gagggttccc
//
LOCUS       DASUTW010000181         4599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_316545, whole genome shotgun sequence.
ACCESSION   DASUTW010000181 DASUTW010000000
VERSION     DASUTW010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316545"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VF058_06335"
     CDS             <1..568
                     /locus_tag="VF058_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HEX6207963.1"
                     /translation="RRDDVDLTVLRFANFIGPGVASTLTRYFSLPLIPTALGYDPRLQ
                     MIHEEDAVEALYRVVRGSHPGTFNVAADGVVLLSQALRLLGRPSLPLALPLATPVASL
                     LRRFGLVDFSTEQLRLLIYGRVADNTRMEREMGFRPARTTREALLDFAEGEHLRRLYS
                     SEQVEEWEREARAFLRRKAQERFEKARS"
     gene            568..1551
                     /locus_tag="VF058_06340"
     CDS             568..1551
                     /locus_tag="VF058_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX6207964.1"
                     /translation="MGEVLPIPVREPSRCTATTRSGARCRNRAVDVSGLCRVHGGGAK
                     EPAAPEGPSTEEIDAFLAFLRRRVTGDYEIDEFGFDRDLTETVLAPLARFFHERYFRV
                     EWKGLEHVPREGPALLVSNHAGTIPMDAVIMKFGLLDRVERHVRLLAANLAFRMPFVG
                     ELARKSGNTLACEEDALRLLERGELLGVFPEGYKGVGKPFRERYKLQRFGRGGFIELA
                     LRAGAPIVPVGIVGSEEIYPLISQARPLARVFGLPYFPVTPFFPWLGPLGLLPLPTKW
                     TIEFRPPIATNGLGEEAWEDSLLVFEMTDRVRDEIQQTLNRNLSARRSLFF"
     gene            complement(1548..2642)
                     /locus_tag="VF058_06345"
     CDS             complement(1548..2642)
                     /locus_tag="VF058_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX6207965.1"
                     /translation="MTAEPRLPLGPGPSPVPTRVLEALARPTLGHLDPDFLQILDETN
                     DLLREVFRTENLAFAVSASGSGGMEAAMVNLLEPGDTVVVGVAGFFAERMAEMARRTG
                     AEVVRVEGTWGRPVAFEDLEAALGQHPETKVMAVVHAETSTGVRQPLEKVAAMCRERD
                     VLLVVDCVASLGGLPVEVDRLGIDVCFSGSQKCLSVPPGLAPISYSAKALAAVEARKT
                     PVQSWYFDVMGVRQYVGAADRRYHHTAPVNMIYGLNEGLKIVLEEGLEARWHRHQEVG
                     GAFQDRIQELGFELFAPEEYRLPQVTSLLIPSGADDAGARKRLIEEHGIEIAGGLGEY
                     RGRMWRVGLMGHGATMENVDLLLDAISAVI"
     gene            complement(2639..2770)
                     /locus_tag="VF058_06350"
     CDS             complement(2639..2770)
                     /locus_tag="VF058_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207966.1"
                     /translation="MRRFTLVTLIVLMALLAIMAVFIAIDVSRGPAPAPTPTEEGER"
     gene            complement(2799..3182)
                     /locus_tag="VF058_06355"
     CDS             complement(2799..3182)
                     /locus_tag="VF058_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6207967.1"
                     /translation="MSVRVLVVDDTEHVRQMMAAMLQIQGFEVVGEAADAKGALELAA
                     ETTPNVIVLDFRMPDVDGITATRWLLEQDADLAIVMYSAYLTEEDEQRALDAGVRACV
                     SKVEGVEALAREVAAAALAVRGERA"
     gene            complement(3179..3979)
                     /locus_tag="VF058_06360"
     CDS             complement(3179..3979)
                     /locus_tag="VF058_06360"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX6207968.1"
                     /translation="MGDGTPKLVLSTAPYYRQPLEQAFRQASAAGFEAVEVMVTQDPD
                     TQDAQTLRDLSKEHELSIEAIHAPFLLFTRRVWTTDPVEKIARGVALAEEAEVPLLVV
                     HPPYRWQARYRRWIGRSLAELSARSGVIIGVENMFPVRVRGAGGVRFHASQDLESLDH
                     YPTLVLDTSHLAVSGFDLLETYQRYREKIVHFHLSNNAGKGWDSHLSVDEGVLPLDEL
                     LRRVGSDGFGGTISIELDLRRTIADEGATREVLVRNREFCERHLGVRA"
     gene            complement(4045..>4599)
                     /locus_tag="VF058_06365"
     CDS             complement(4045..>4599)
                     /locus_tag="VF058_06365"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6207969.1"
                     /translation="GRERPSATKIAALVVGLLGVLLVLGTPTGSLDPAGVSLALASAL
                     CLAIYIIVAQRGAAGMSPLVAGGVILSVSTLVYVPIAFLTGLLPGEGLGGGPSFVAGV
                     GLATGAAIALFLAGLARLEPIRASIASTWEPVAAVILSAIVLGERLSGLQLLGGTLVV
                     AAVAALPLIREAEDATTPPGPPIA"
BASE COUNT          658 a         1643 c         1630 g          668 t
ORIGIN      
        1 tcgccgggac gacgtggacc tcaccgtcct tcgcttcgcc aacttcatcg ggcccggggt
       61 ggcctcgacc ctcacgcgct acttctccct cccgttgatc cccacggccc tcggctacga
      121 cccgaggctc cagatgatcc atgaggagga cgcggtggag gccctgtatc gggtggtccg
      181 tgggtcccat ccggggacct tcaacgtggc cgccgacggg gtggtgctgc tctcgcaggc
      241 cctccggctg ctggggaggc cctcactgcc gctggccctt ccgctcgcca cgccggtggc
      301 gtcgctgctg cgccggttcg ggctcgtcga cttctccacc gagcagctcc gcctgctgat
      361 ctacgggcgc gtggcggaca acacgaggat ggaacgggag atgggcttcc gccccgcgcg
      421 cacgacccgc gaggccctgc tcgacttcgc cgagggggag cacctccggc ggctctacag
      481 ctccgagcag gtcgaggagt gggagcgcga ggcccgcgcg ttcctgcggc gcaaggctca
      541 ggagcggttc gagaaggcga ggagctgatg ggcgaggtcc tcccgatccc tgtgcgcgag
      601 ccgagccgct gcacggcgac cacgaggagc ggggcgcgct gccgcaaccg ggcggtggac
      661 gtctccggcc tgtgtcgcgt gcacggcggc ggcgcgaagg agccggcggc tcccgagggc
      721 ccctccaccg aggagatcga cgcgttcctc gccttcctcc ggcgccgcgt caccggcgac
      781 tacgagatcg acgagttcgg cttcgaccgc gacctcaccg agacggtcct cgcgccgctg
      841 gcccgcttct tccacgagcg gtacttccgg gtcgagtgga agggcctgga gcacgtgccg
      901 cgggaggggc cggccctgct cgtctcgaac cacgccggga ccatccccat ggacgcggtg
      961 atcatgaagt tcgggctgct cgatcgggtg gagcgtcacg tgcggctgct ggcggcgaac
     1021 ctcgcgttcc ggatgccgtt cgtgggggag ctcgcgcgga agtcgggcaa cacgctggcg
     1081 tgtgaggagg acgccctgag gctcctcgag cgaggcgagc tgctcggcgt gttccccgag
     1141 gggtacaagg gggtcggcaa gcccttccgc gagcggtaca agctccagcg gttcgggcgc
     1201 ggtgggttca tcgagctggc gctgcgcgcg ggggctccga tcgtccccgt ggggatcgtg
     1261 gggagcgagg agatctaccc gctgatctcg caggcccgac ccctggcccg ggtgttcggg
     1321 ctgccctact tcccggtgac gccgttcttc ccgtggctgg ggccgctcgg gttgctgccc
     1381 ctgcccacga agtggacgat cgagttccgc ccgccgatcg ccacgaacgg gctcggcgag
     1441 gaggcctggg aggactcgct gctggtgttc gagatgacgg accgagtgcg ggacgagatc
     1501 cagcagaccc tcaacaggaa cctctccgcc cgtcgctcgc tcttcttcta gatcacggcc
     1561 gagatcgcgt cgaggaggag gtcgacgttc tccatcgtcg caccgtggcc catcagtccc
     1621 acgcgccaca tgcggccgcg gtactcgccg aggccgccgg cgatctcgat gccgtgctcc
     1681 tcgatcagcc gcttccgggc gccggcgtcg tcggccccgg acgggatcag gagtgacgtc
     1741 acttggggca gccggtactc ctccggcgcg aacagctcga agccgagctc ctggatccga
     1801 tcctggaagg cgcctcccac ctcctggtgc ctgtgccacc gagcctcgag cccctcctcc
     1861 aggacgatct tcagcccctc gttcagcccg tagatcatgt tgaccggcgc cgtgtggtgg
     1921 tagcggcggt cggccgcccc cacgtactga cgcaccccca tcacgtcgaa gtaccaggac
     1981 tggaccggcg tcttccgggc ctccaccgcc gccagcgcct tggccgagta cgagatcggg
     2041 gccaggcccg gcggcaccga gagacacttt tggctccccg agaagcagac gtcgatcccc
     2101 agtcggtcga cctcgacggg cagacctccc agcgaggcca cgcagtccac gacgaggagc
     2161 acgtcccgct cccgacacat ggcggcgacc ttctcgaggg gctgtcgcac gccggtcgag
     2221 gtctccgcgt gcacgaccgc catcaccttc gtctcggggt gctggcccag cgccgcctcg
     2281 aggtcctcga acgccacggg acgtccccac gtaccctcca cgcggaccac ctctgccccc
     2341 gtcctcctcg ccatctccgc catgcgctcg gcgaagaacc cggccacgcc cacgaccacc
     2401 gtgtcgccgg gctccaggag gttgaccatc gccgcctcca tcccgccgct gccgctcgct
     2461 gagacggcga aggcgaggtt ctcggtgcgg aagacctcgc ggaggagatc gttcgtctcg
     2521 tcgaggatct ggaggaagtc ggggtcgagg tggccgaggg tgggccgagc cagcgcctcg
     2581 aggacgcgcg tggggacggg cgaggggccg ggcccgagcg gcaggcgagg ctcagcggtc
     2641 atcgttctcc ctcctcggtc ggcgtcgggg ccggcgccgg cccccgcgac acgtcgatcg
     2701 cgatgaacac cgccatgatc gccagcagcg ccatcagcac gatcagcgtc acgagcgtga
     2761 accggcgcac cgcccagatg ctaacaagcg gtcccggctc aggcccgctc gcctcggact
     2821 gcgagcgccg ccgcggccac ctcgcgagcc agcgcctcga cgccctccac cttggagacg
     2881 cacgcgcgca cccctgcatc cagggctcgc tgctcgtctt cctcggtgag gtaggccgaa
     2941 tacatgacga tcgccaggtc ggcgtcctgc tccagcagcc accgcgtcgc ggtgatgccg
     3001 tccacgtcgg gcatgcggaa gtcgaggacg atgacgtttg gggtcgtctc ggccgccagc
     3061 tcgagggctc ccttcgcgtc ggccgcctcg cccaccacct cgaacccctg gatctgcagc
     3121 atcgccgcca tcatctggcg gacgtgctcg gtgtcgtcca cgacgagcac ccggacgctc
     3181 acgcccgcac cccgaggtgc cgctcgcaga actcgcggtt ccgcacgagg acctcgcggg
     3241 tcgcgccctc gtccgcgatc gtcctgcgga ggtccagctc gatcgagatc gtgccaccga
     3301 agccgtcgga gccgacccgg cgcagcagct cgtcgagcgg gaggacgccc tcgtccacgg
     3361 agaggtgcga gtcccagccc ttccccgcgt tgttggagag gtggaagtgg acgatcttct
     3421 cccggtaccg ctggtaggtc tccaggaggt cgaagccgga cacggccagg tggctcgtat
     3481 cgagcacgag ggtggggtag tggtcgaggc tctccagatc ctgggaggcg tggaagcgca
     3541 ccccgcccgc gccgcggacc ctcacgggga acatgttctc gacgccgatg atcacgccgg
     3601 agcgggcgga gagctccgcc aggcttcggc cgatccaccg ccggtagcga gcctgccacc
     3661 ggtagggcgg gtgtacgacg aggagcggga cctccgcctc ctcggcgagc gcgacgccac
     3721 gcgcgatctt ctccacgggg tcggtggtcc agacgcgccg ggtgaagagg aggaagggcg
     3781 cgtggatcgc ctcgatcgac agttcgtgct ccttggagag gtcgcgaagg gtctgggcgt
     3841 cctgggtgtc cgggtcctgg gtcaccatga cctcgaccgc ctcgaagccg gccgcgctgg
     3901 cctgtctgaa ggcctgctcc agcggctgtc ggtagtacgg ggcggtcgag aggaccagct
     3961 tgggcgttcc gtctcccacg ttcctcatcc tctccggggt cgtcattcta cagtggcccc
     4021 tcgcgggttc gaggacgctc ggcttcaggc gatgggagga ccgggcgggg tggtggcgtc
     4081 ctcggcctcc cggatcaggg ggagcgcggc caccgcggcc acgaccagcg tcccgccgag
     4141 gagctggagg ccggacagcc gctcgcccag gacgatcgcc gagaggatga cggccgcgac
     4201 gggctcccag gtggacgcga tcgaggcccg gatcggctcg aggcgggcca gcccggccag
     4261 gaagagggcg atggcggccc ccgtggccag gccgaccccg gccacgaacg agggcccgcc
     4321 gccgagcccc tcgcccggca gcagcccggt gaggaacgcg atggggacat agaccagcgt
     4381 ggagacggag aggatcacgc cgccggccac caggggcgac atcccggccg cgccgcgctg
     4441 ggccacgatg atgtagatgg cgaggcagag ggccgaggcc agggccaggg acacgcccgc
     4501 cgggtcgagg ctgccggtgg gggtccccag gacgaggagg acgcccagga ggcccaccac
     4561 gagggcggcg atcttcgtcg cgctcgggcg ctcgcgccc
//
LOCUS       DASUTW010000182         2468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_316764, whole genome shotgun sequence.
ACCESSION   DASUTW010000182 DASUTW010000000
VERSION     DASUTW010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316764"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1594)
                     /locus_tag="VF058_06370"
     CDS             complement(<1..1594)
                     /locus_tag="VF058_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207970.1"
                     /translation="MRSVSRLLPLALTLGLLLPGGPVQGDETVGPGGGTPGTSSNFEL
                     VGHDPLFNRGMNAALAVYEDFVYVGNRTDGSNRCGLGDPRREELGLDECPHINPGILV
                     VDASDPSAPEVVDEIGPPHAGNVRETTRELRVSPREELLIVATFGCSSVIHSCVPGGV
                     VPSLRFFDVSNPADPTHVLTWAPRQANELIRTPHEFYLWEDPQDPDRTLLWISTPTGN
                     RNPVNANLLIADISDVDEGEQPTLIAQGQWNHMFPPMGNVALHSMTPTVDGRTTHLAY
                     LSGFFLILDTSEVAEGNPPEGEVIDLNDDLLTPVENRPTWDNPNPGHSAVPFLGRPFS
                     LITDEIYGTFTSGAFGCPWGWARTAQVAQPNKPHVIGEYRITENSCPPPTQADQERGS
                     YSSHNPTLTRNLGLVTWHSGGLQAIDISDPAAMQQAGWYSPVPLESVATEDPALSMGT
                     NKVVMWSFPVIKDGLIYVVDIRNGLYVLRYTGPHAREVAGIEFLEGNSNLGDALRLAK
                     SAPPLSSSRGRRSWASRRPSAGT"
     gene            complement(1749..>2468)
                     /locus_tag="VF058_06375"
     CDS             complement(1749..>2468)
                     /locus_tag="VF058_06375"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6207971.1"
                     /translation="AEALLGDAKRVVELDFGQFDRAEDVNTLLGAPPAYIGFGEHLPL
                     HDLSRMPWSVVLCKNVDGCHPQVREVLAQALSDGFLTERSGKRVYLSDAVVVLTAPSE
                     GAGRLSPGFLSEEGTSSMERVLGARLLEQVDVVSTTRPVQETEGSGWLEGTLLDGLSE
                     QYREDGLDVEWGASFLAWVRDQQPSLPSRLMLTRLVERRLGEALIPRLPAPGARAAVL
                     VEAEDGELRVTDRGGTESEGE"
BASE COUNT          363 a          848 c          865 g          392 t
ORIGIN      
        1 aggtaccagc cgagggtcgc cgcgaggccc aggaccgtcg tccccgggag gaggaaaggg
       61 gcggcgcgga cttggcgagc ctcagcgcgt cgccgaggtt cgagttcccc tcgaggaact
      121 cgatccccgc cacctcacgc gcgtgggggc ccgtgtaccg gagcacgtag aggccgttcc
      181 ggatgtccac gacgtagatc agcccgtcct tgatgaccgg gaagctccac atcacgacct
      241 tgttcgtgcc catgctgagg gccgggtcct cggttgctac ggactccagc ggcaccggtg
      301 agtaccaccc agcctgctgc atcgccgcgg ggtccgagat gtcgatcgcc tggaggccgc
      361 ccgagtgcca ggtcaccagc ccgagattcc tcgtcagcgt cgggttgtgt gagctgtacg
      421 agcctcgctc ctggtccgcc tgggttggag gcgggcagga gttctcggtg atccggtact
      481 cgccgatcac atgcggctta ttcggctggg ccacctgtgc ggtgcgggcc cacccccacg
      541 ggcagccgaa ggcgccgctg gtgaacgtgc cgtagatctc gtccgtgatg agcgagaacg
      601 gcctcccgag gaacggcacc gccgaatgcc cggggttggg gttgtcccac gtcggccggt
      661 tctccacggg cgtgaggaga tcgtcgttca ggtcgatgac ctccccctcg ggcgggttcc
      721 cctccgcgac ctcgctcgtg tccaggatca ggaagaaccc ggagaggtac gcgaggtggg
      781 tcgttctccc gtccacggtc ggggtcatgg agtgcagcgc gacgttcccc atcggaggga
      841 acatgtggtt ccactgcccc tgggcgatca gcgtgggctg ctcgccctcg tcgacgtcgc
      901 tgatgtcggc gatcagcagg ttcgcgttga cggggttccg gttgcccgtc ggcgtcgaga
      961 tccagagcag cgtacggtcc gggtcctgcg ggtcctccca gaggtagaac tcgtgtgggg
     1021 tcctgatcaa ctcgttcgcc tgccgcggcg cccacgtgag gacgtgcgtc gggtccgccg
     1081 ggttggagac gtcgaagaac cggaggctgg ggacgacgcc accggggacg caggagtgga
     1141 tcacgctgct gcatccgaac gtcgccacga tcaggagttc ctcacgcggg gagacccgga
     1201 gctcgcgcgt cgtctcacgc acgttcccgg cgtggggggg tccgatctcg tcgacgacct
     1261 cgggcgcgga gggatcggag gcgtccacga cgaggatccc cgggttgatg tgcgggcact
     1321 cgtcgagccc gagctcctcg cgtctcgggt cgcccagacc gcaccggttc gagccgtcgg
     1381 tcctattccc cacgtagacg aagtcctcgt acacggccag ggcggcgttc atgcccctgt
     1441 tgaacagcgg gtcgtgcccc accagctcga agttcgagga cgtgccgggg gtccctccac
     1501 cgggaccgac ggtctcatcg ccctgcaccg gcccgccggg cagcagcagg ccgagcgtga
     1561 gagcaagggg gagcagacgg gagaccgagc gcataggcca cctccttcgg cggccgcccc
     1621 atcgcgattg ggcggctcgc gatgtcttct ttcggacggc tcatgaccct atgggggggt
     1681 ttacgaagag tcaaatcggc ttcaccccac caccttcggg cgcggcccca gcgcgagtcc
     1741 tccgcgcctc actccccctc cgactccgtg ccgccccggt cggtcacccg cagctcgccg
     1801 tcctccgcct cgacgagcac ggcggcccgc gccccgggcg cggggaggcg cgggatcaga
     1861 gcctctccca ggcgtcgctc gaccagtcgg gtgagcatca gcctcgacgg cagcgagggc
     1921 tgctggtccc ggacccaggc caggaaggag gctccccact ccacgtccaa cccgtcctct
     1981 cggtactgct ccgagagtcc gtcgaggagc gtgccctcga gccatcctga gccctcggtc
     2041 tcctggaccg gcctggtggt gctcacgacg tcgacctgct cgaggagcct ggcgccgagg
     2101 acccgctcca tcgaggaggt cccctcctcg gagaggaagc ccggggagag acgtcccgcg
     2161 ccctcggagg gcgcggtcag gaccacgacg gcgtcgctga ggtacacgcg cttcccgctc
     2221 cgctcggtca ggaatccgtc ggagagggcc tgggcaagga cctcgcggac ctgggggtgg
     2281 cagccgtcca cgttcttgca caggacgacg ctccagggca tccgggacag gtcgtgcagc
     2341 gggaggtgct cgccgaaccc gatgtaggcg ggcggggcgc caaggagcgt gttcacgtcc
     2401 tcggctcggt cgaattgccc gaagtccaac tcaacgacgc gcttcgcgtc gccgaggagg
     2461 gcctccgc
//
LOCUS       DASUTW010000183         2034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_57933, whole genome shotgun sequence.
ACCESSION   DASUTW010000183 DASUTW010000000
VERSION     DASUTW010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57933"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..657)
                     /locus_tag="VF058_06380"
     CDS             complement(55..657)
                     /locus_tag="VF058_06380"
                     /inference="COORDINATES: protein motif:HMM:NF015743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017128 - phospholipid scramblase activity
                     [Evidence IEA];
                     GO_process: GO:0017121 - plasma membrane phospholipid
                     scrambling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid scramblase-related protein"
                     /protein_id="HEX6207972.1"
                     /translation="MSLLERSRMFVHQKPKLIELTNEYAILGEDGEQIGFIRQEGQSK
                     ARKVLRFVSSLDQYLTHTYAMYDADGTKVLELTRPRKLLKSRFLVSDGAGLPVGTVVQ
                     ENVVGRKRFRLLGQSGEELGSFQAENLISWDFQILDPARVPVGRVTKKWKGLGIEALT
                     TADNYMVEIDDSVEGPFRMLAVASAASIDTALKQDAQGFN"
     gene            809..>2034
                     /locus_tag="VF058_06385"
     CDS             809..>2034
                     /locus_tag="VF058_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6207973.1"
                     /translation="MKDTPYWNPRHETMPREELEALQVRKLRNLVAWAGAQVPFHAKR
                     LSDAGVTADSIQSLDDLRRVPFFTRDEWMQGQLDQPPFGPILSAPEEAAIRYHMTSGT
                     TGRTPIRVLDSMKDWEWIAEMWCYGLWGFGVRPTDRMFVAFGYATFIGFWGLHYAGEK
                     MGCLTLPGGAMTTEQRVRTIEEMGATVVASTPTYALRMAQEARALGIDLASGPVQRLI
                     LSGEPAGSIPATKKLIEEQWGAKAGDTAGMTELGTIMIFECEAQPGGTHIIEDHYVEE
                     VIDPQTGEPVDYGEQGERVVTSFGRGFIPVLRYRTRDLVVKVPASACSCGRTFDLYDG
                     GIRGRVDDMKLVRGTNVYPRAVEAIVREIPEIDEFQIHLYTAEGIRDEIEVLVEIPDE
                     GADVDGILARLGKELA"
BASE COUNT          328 a          677 c          721 g          308 t
ORIGIN      
        1 tcaccgtggc gaggatgggg accaagcgcc gagggcccga ggaggatcgc cccgtcagtt
       61 gaagccctgc gcgtcctgct tcagggcagt gtcgatgctg gccgccgatg ccaccgccag
      121 catccggaac ggcccttcca ccgaatcgtc gatctcgacc atgtagttgt cggcggtcgt
      181 gagcgcctcg atcccgaggc ccttccactt cttggtcaca cgccccacgg ggaccctggc
      241 ggggtcgagg atctggaagt cccacgagat caggttctca gcctggaacg agcccagctc
      301 ctcgccggac tggcccagca gccggaagcg cttcctcccc accacgttct cctgcaccac
      361 cgtgccgacg ggcagtccgg ctccgtcgct gaccaggaac ctcgacttca ggagcttgcg
      421 gggacgcgtg agctcgagca ccttcgtgcc gtcggcgtcg tacatcgcgt aggtgtgcgt
      481 caggtactgg tcgagcgagg agacgaagcg cagcactttc ctcgccttcg actgaccctc
      541 ctggcggatg aagccgatct gctccccgtc ctcgcccagg atcgcgtact cgttcgtgag
      601 ctcgatcagc ttgggcttct ggtgcacgaa catgcgagag cgctcgagca gcgacaagag
      661 ggcctccctt cgacgaggag aagcctaccc cacacccggg ggctcaggct ccgctcgagg
      721 cgccgcggtc ggcggggggc ctgctctcgc tcgcacgctc gcagggcccg tcggtaggat
      781 gtcgccgacc gagccgggga gggtgcagat gaaggacacg ccgtactgga acccccgtca
      841 cgagacgatg ccccgagagg agctcgaggc gctgcaggtt cggaagctcc ggaacctcgt
      901 ggcgtgggcg ggcgcccagg ttcccttcca cgccaagcgg ctctccgacg cgggggtgac
      961 cgcggactcg atccagagcc tcgacgacct ccgccgggtc cccttcttca cccgcgacga
     1021 gtggatgcag ggccagctcg accagccccc cttcggcccg atcctctcgg cgcccgagga
     1081 ggccgcgatc cgctaccaca tgacctccgg gacgaccggc cgcacgccga tccgcgtgct
     1141 cgactcgatg aaggactggg aatggatcgc ggagatgtgg tgctacgggc tgtggggctt
     1201 cggggtgcgg cccaccgacc gcatgttcgt ggcgttcggc tacgcgacct tcatcggctt
     1261 ctggggtctc cactacgcgg gcgagaagat gggctgcctc acgctgcccg gcggcgcgat
     1321 gaccaccgag cagcgggtcc ggacgatcga ggagatgggg gcgacggtgg tggcctccac
     1381 cccgacgtac gccctgcgga tggcccagga ggcccgggcg ctggggatcg acctggcgag
     1441 cgggccggtg cagcggctga tcctctccgg cgagcccgcc ggctcgatcc ccgccacgaa
     1501 gaagttgatc gaggagcagt ggggggcgaa ggccggcgac accgccggga tgaccgagct
     1561 gggcacaatc atgatcttcg agtgcgaggc ccagcccggc gggacccaca tcatcgagga
     1621 ccactacgtc gaggaggtca tcgacccaca gaccggagag ccggtggact acggggagca
     1681 gggagagcgg gtagtgacct cgttcggccg ggggttcatc ccggtgctgc ggtaccggac
     1741 ccgtgacctc gtggtgaagg tgccggcctc ggcgtgctcg tgcgggcgga ccttcgacct
     1801 ctacgacggc ggcatccggg ggcgcgtgga cgacatgaag ctggtccgcg ggacgaacgt
     1861 ctacccgcgg gcggttgagg cgatcgtccg cgagatcccc gagatcgacg agttccagat
     1921 ccacctctac acggcggagg ggatccggga cgagatcgag gtcctggtgg agatccccga
     1981 cgagggggcc gatgtggacg ggatcttggc caggctgggg aaggagctgg ccga
//
LOCUS       DASUTW010000184         3831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_705791, whole genome shotgun sequence.
ACCESSION   DASUTW010000184 DASUTW010000000
VERSION     DASUTW010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705791"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VF058_06390"
     CDS             <1..361
                     /locus_tag="VF058_06390"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6207974.1"
                     /translation="RWDPDMELEQISEGPMTLGTRIRRRNVRWGDPVEGEMEVVEWEP
                     ERSVGFHIRDANMEMRGRAAFQDDGPGRTLLTVLTDVPELDESKASFLRSMMERSLAN
                     VKRLAESDIERASRSGG"
     gene            381..1367
                     /gene="lipA"
                     /locus_tag="VF058_06395"
     CDS             381..1367
                     /gene="lipA"
                     /locus_tag="VF058_06395"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX6207975.1"
                     /translation="MRFRDPSRDKNPRRKRLHLPIAEVGAPDTHTVHSVGCAQQVVPQ
                     RKPSWMRAHLRTGPNYRDLKGLMEGLSLNTVCEEAGCPNIFECWEQREATFLILGNTC
                     TRRCGFCDIQTGRPAPVDEEEPSRIAEAVSAMGLNHVVLTGVARDDLPDGGASIWAAC
                     IRAVRETVPGCGIEILPPDFRAHREQIATVIAEEPDVFAHNLETVRRLHDRIRPGFDY
                     DRTLDALRLAEELRPGQVTKSNLILGMGERPEEVEGALRDLRDARVDLMTIGQYLRPT
                     TFHLPVDRWVTPEEFEEHARVGRRLGFAHVESGPLVRSSYRAGKQLAAARRS"
     gene            1384..2475
                     /locus_tag="VF058_06400"
     CDS             1384..2475
                     /locus_tag="VF058_06400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6207976.1"
                     /translation="MITLRHVSKVYDGSAPAVDNLSLEIEDGQIVVLVGPSGCGKTTT
                     LKMINRLIEPTSGEIVVNGRNVLDQDPVELRRGMGYVIQSIGLLPHRTIRQNIATVPD
                     LLGWDRQRIQARINELAEMLRLDPGLLDRYPGELSGGQRQRVGVARGLAADPPVMLMD
                     EPFGAVDPIIRERLQDQFLEIQRELKKTIVFVTHDVDEAIKMADRIAILNIGAVVEQY
                     DAPEAILSEPASEFVKEFVGLERGLKRLSLIKVADIEVEEGPMVAPTDDVPTALAEME
                     RSGVGWASVIEEGRLLGWVDPGCLEGMTSVGQYPPRRFSATVTDRSSLREALDTIVTS
                     RTNVAVVVTDGGRYRGVLTLEKLTRELIA"
     gene            2472..3146
                     /locus_tag="VF058_06405"
     CDS             2472..3146
                     /locus_tag="VF058_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6207977.1"
                     /translation="MTGRPFWDWDWVGRNGDLIVDQAVQHLWLTFLAVVIGLAISFPL
                     AVLAYRRRRLYPPIAWLTGTLYTIPSLALFAFLTPFTGLSTLTAEIGLVSYTLLILIR
                     NIVTGLRAVPPEVREAALGMGYTDRQLLWRIELPLALPAVVAGLRIATVTTIGLVTVT
                     ALIGQGGLGALMLGGFRRLYPELAVVLGVGLSVALAVAVDLLLFAGQRWITPWSRRAG
                     DRVIAT"
     gene            3143..>3831
                     /locus_tag="VF058_06410"
     CDS             3143..>3831
                     /locus_tag="VF058_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018892486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6207978.1"
                     /translation="MTEPGFFGQVVDWFTDAANWSGDFGIPNRLLEHVVMSGVSVAIA
                     TALALPLAMYIGHRRRLERVVVSVTNLGRAIPSFGLLFLFALWLGVGLSSPPALRPAI
                     ILALVLLAIPPILTNAYVGIQSVDDDTLEAARGMGMSEWGILFRVEIPLGMPLIVAGL
                     RTAAVQVVATATLGAVVAGGGLGRYIIDGFAVRDLAQIFAGAVLVAALAILVDVLIGG
                     VGRLLSPRVATR"
BASE COUNT          556 a         1325 c         1335 g          615 t
ORIGIN      
        1 ccggtgggac ccggacatgg agctcgagca gatctccgag ggcccgatga cgctgggcac
       61 gaggatccgt cggaggaacg tccgctgggg cgaccccgtc gagggcgaga tggaggtcgt
      121 ggagtgggaa ccggagcgct cggtcggatt ccacatccgc gacgccaaca tggagatgcg
      181 cggacgagca gcgttccagg acgacgggcc cggcagaacg ttgctcacgg tcctgaccga
      241 cgttccggag ctggacgagt cgaaggcaag cttcctgcgc agcatgatgg agcggagcct
      301 cgcgaacgtg aagcgcctgg ccgagtcgga catcgagcgg gcctctcggt cggggggttg
      361 accggctaga ctggccctcg atgcggttcc gcgaccccag tcgagacaag aacccgcggc
      421 gcaagcggct tcacctcccg atcgcggagg tgggcgcccc agatactcac accgtgcact
      481 cggtcggctg cgcgcagcag gtcgtccctc agcgcaagcc ttcctggatg cgggcccacc
      541 tccgcaccgg cccgaactac cgcgacctga aggggctcat ggagggcctc tcgctcaaca
      601 ccgtctgcga ggaggccggc tgcccaaaca tcttcgagtg ctgggagcag cgcgaggcca
      661 ccttcctgat cctcgggaac acctgcaccc gtcgctgcgg cttttgcgac atccagaccg
      721 ggaggcctgc tccggtggac gaggaagagc cctcccgcat cgcggaggcc gtctccgcga
      781 tggggctcaa ccacgtcgtc ctcactgggg tggcgcgcga cgacctcccc gatggcgggg
      841 cgagcatctg ggcggcctgc atccgcgccg tccgcgagac cgtcccgggg tgcggcatcg
      901 agatcctccc gcccgacttc agggcccacc gcgagcagat cgcgaccgtg atcgccgagg
      961 agcccgacgt cttcgctcac aacctcgaga ccgtacggcg tctccacgac cgcatccgtc
     1021 ccgggttcga ttacgaccga acgctcgacg cccttcggct cgccgaggag ctccgcccgg
     1081 gtcaggtgac gaagtcgaac ctgatcctgg ggatgggcga gcgccccgag gaggtcgagg
     1141 gcgcccttcg cgacctgcgt gacgcgcgcg tcgatctcat gacgataggc cagtacctcc
     1201 ggccgacgac gttccatctc cccgtcgatc gctgggtaac ccccgaggag ttcgaggagc
     1261 acgcccgggt aggccgtcgg ctcgggttcg cccacgtgga gtcgggccct ctcgtgcgtt
     1321 cctcctatcg ggccgggaag cagctggccg ctgcgcggcg ttcctgaacc gtaggagaga
     1381 cccgtgatca cgctccgcca cgtgtcgaag gtctacgacg gctcggcccc cgcggtcgac
     1441 aatctttcgc tggagatcga ggacggccag atcgtcgtcc tcgtcggccc gtcgggctgc
     1501 gggaagacga ccacgctgaa gatgatcaac cggctcatcg agcccacctc tggcgagatc
     1561 gtcgtcaacg gacgcaacgt cctcgaccag gaccccgtgg agctgcggcg tggcatgggg
     1621 tacgtgatcc agtcgatcgg tctgctcccc catcgaacga ttcgccagaa catcgccacg
     1681 gtgcccgatc tgctgggatg ggatcgccag aggatccagg cccgcatcaa cgagctcgcc
     1741 gagatgctcc gcctcgatcc cgggctgctc gaccgctacc cgggcgagct ctccggcggc
     1801 cagcgccagc gggtcggcgt cgcccgggga ctggccgcgg accccccggt gatgctgatg
     1861 gacgagccgt tcggcgccgt ggaccccatc atccgcgagc gactccagga ccagttcctg
     1921 gagatccagc gcgagctgaa gaagaccatc gtgttcgtca cccacgacgt ggacgaggcg
     1981 atcaagatgg ccgaccgcat cgccatcctc aacatcgggg ccgtggtcga gcagtacgac
     2041 gcccccgagg cgatcctgtc cgagcccgcc agcgagttcg tgaaggagtt cgtggggctg
     2101 gagcgggggc tgaagcggct gtcgctgatc aaggtggccg acatcgaggt ggaggagggg
     2161 ccgatggtcg cccccaccga cgacgtgccc accgcgctcg ccgagatgga gcgctcgggc
     2221 gtcggatggg cctctgtgat cgaggagggg cggctgctgg gctgggtcga tcccggatgc
     2281 ctcgagggga tgacctcggt gggccagtac cctccccgcc gcttcagcgc gaccgtgacc
     2341 gaccggagct ccctcaggga ggccctggac acgatcgtca cgtcccggac gaacgtggcg
     2401 gtggtcgtca cggacggcgg ccgctaccga ggcgtcctga cgctggagaa gctcaccagg
     2461 gagctgatcg cgtgacgggc cgtcccttct gggactggga ctgggtgggc cggaacggtg
     2521 acctcatcgt ggaccaggcc gtgcagcacc tctggctcac gttcctggcc gtggtgatcg
     2581 ggctggcgat ctcgttcccg ctggcggtgc tggcctatcg tcgccgcagg ctctatcccc
     2641 cgatcgcgtg gctcaccggc accctctaca cgatcccgag cctcgccctc ttcgccttcc
     2701 tgaccccctt caccggcctc agcacgctca cggctgagat aggcctcgtg tcgtacacgc
     2761 tcctcattct gatccggaac atcgtgaccg gtctccgggc cgtgccgccc gaggtccgag
     2821 aggcggcgct cgggatggga tacacggacc gccagctcct gtggaggatc gagctccccc
     2881 tcgcgctgcc cgcggtggta gccggcctcc gcatcgcgac cgtgaccacg atcgggctcg
     2941 tcaccgtcac cgcgctgatc ggccagggcg gcctcggagc gctgatgctg ggcgggttcc
     3001 gacgcctcta cccggagctc gccgtggtgc tcggcgtcgg gctgtcggtg gcgcttgccg
     3061 tcgcggtcga cctcctgctg ttcgccggcc agcgatggat cacgccctgg agccggcgcg
     3121 ccggggatcg ggtgatcgcc acgtgaccga gcccgggttc ttcggccagg tcgtggactg
     3181 gttcaccgac gcggccaact ggagcgggga cttcgggatc cccaacaggc tcctggagca
     3241 cgttgtgatg tcgggcgtct cggtggcgat cgcgacggcg ctcgccctgc cgctggcgat
     3301 gtacatcggg catcggcggc ggctggagcg ggtcgtggtc tcggtcacca acctcgggag
     3361 ggcgatcccc tccttcgggc tgctgttcct cttcgcgctg tggctgggcg tggggctctc
     3421 gagccccccc gctctgcggc ccgcgatcat cctggcgctc gtcctcctgg cgatcccgcc
     3481 gatcctcacg aacgcctacg tcggcatcca gagcgtggac gacgacacgc tggaggcggc
     3541 ccgagggatg ggcatgtccg agtgggggat cctcttccgg gtcgagattc ctctcgggat
     3601 gcccctgatc gtggcgggcc ttcggacggc ggcagtccag gtcgtggcca ccgccacgct
     3661 cggcgccgtg gtggccggtg gagggctggg ccgctacatc atcgacggct tcgcggttcg
     3721 ggacctcgcc cagatcttcg cgggagcggt cctcgtcgcg gcgctggcga tcctggtgga
     3781 cgtgctgatc ggaggggtcg gccggctcct ctcgccgcgc gtggccacgc g
//
LOCUS       DASUTW010000185         7815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_186042, whole genome shotgun sequence.
ACCESSION   DASUTW010000185 DASUTW010000000
VERSION     DASUTW010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7815
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186042"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VF058_06415"
     CDS             complement(<1..689)
                     /locus_tag="VF058_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007465498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX6207979.1"
                     /translation="MRRTPVFEPGTGSFGIDDPLVLKLEQLQHTGSFKPRGAFNRILA
                     NEVPKAGVIAASGGNHGLGVAFAAARLGVAAEIFIPDTSPETKIEGIRELGATVHVVP
                     GYYAEALEASRSRAEETGALVVHAYDQPEVVAGQGTIGLELAEQAPDLDSVLVAVGGG
                     GLIGGVCTWFGNDVRVIGVEPERCPTLHTAREVGEPVDVEVGGVAADALGAARAGRHG
                     FEAARRYVERVV"
     gene            complement(788..2707)
                     /locus_tag="VF058_06420"
     CDS             complement(788..2707)
                     /locus_tag="VF058_06420"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX6207980.1"
                     /translation="MAENLTRKILGQHLADGEMTPGQPIRFSIDQVLLQDATGTMAWM
                     EFEQLGADRVHPPTVVQYVDHNMIQLDHKNPDDHFFLQTCAMRYGAWFSRPGNGISHY
                     VHLERFDVPGQTMIGSDSHTTTGGAMGMFALGVGGMDAAVVMAGHPFELAMPSVVRVV
                     LENELSPWVSAKDVILEMLRRLSVKGGVGKAFEFAGPGVDTLSVPERATICNMILELG
                     ATGGLFPSDRHTKDWLTAQERADDFVELQADDGASYDEEMVIDLARLEPLIALPSNPD
                     NVVPVREVAGTKVAQVCVGSSVNSSYEDLAIPAAIVKEGGVDEWLDMTVSPGSRQILD
                     TISRTGVLAGYIAAGARLLEPACGPCVGMGQAPPSDQPSVRTMNRNFPGRSGTVDDRV
                     YLASPAVAGATALRGVITDPRDLGMDPPKIDEPPMVVDDRMLIPPQPEEERAKVEIWR
                     GPNIVPPPLPPDLPVALEGRVLIVLGDNISTGSLSPDGVIVMGERSNIARIAEYTLQK
                     EDPTFVARAREWGGGFIVAGDSYGQGSSREHAALAPLELGVRAVFATGIHRIHRKNLI
                     NVGILPLLIDEDLQKTAQQGQVWRLPRVREELAWGEITVEAEGRQHIARAPLSPSERD
                     ILLAGGLIRYLKEQA"
     gene            2811..3146
                     /locus_tag="VF058_06425"
     CDS             2811..3146
                     /locus_tag="VF058_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207981.1"
                     /translation="MEALMATVRTVVDLIVRGEFETLESMTRGRRLTASELRASVEAY
                     GRKLARPPEEAWEEIDVVEVESESRIYNVHIPLWTEEEGRSDLTLELTLTEFAPGLFS
                     IEVDGIHVL"
     gene            3212..3631
                     /locus_tag="VF058_06430"
     CDS             3212..3631
                     /locus_tag="VF058_06430"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX6207982.1"
                     /translation="MSVVEHLREAAPGVLEDEPVVFAYLFGSQATGQAHPRSDVDVAV
                     MLDETVPEDERLETSLRLARRLSEASGLGRIEVVLLNDAPLPLVGRAISTRQVIYSKD
                     EPARVEFESRRHREFLDFQIHAEPLARALLRRAAGER"
     gene            3631..4041
                     /locus_tag="VF058_06435"
     CDS             3631..4041
                     /locus_tag="VF058_06435"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HEX6207983.1"
                     /translation="MVDVGRVLALLERLQAETDELAKLRDSEALPGDETALAAAKYRF
                     IVAIGTCIDVAEHVIASEGLRSPDSFAEAFAVLGDVSFIEEDLAADLQDMARFRNLLV
                     HGYQRVDDARVAEILRTRLDDFERFRAAIARVVS"
     gene            complement(4038..6731)
                     /gene="acnA"
                     /locus_tag="VF058_06440"
     CDS             complement(4038..6731)
                     /gene="acnA"
                     /locus_tag="VF058_06440"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX6207984.1"
                     /translation="MTEQLETSGGTIRYVPLTRAADETVLERLPYVIRVFLENALRKQ
                     GEGTEAEHVEALAGWPEAARREIPFFPGRVVLQDFTGVPVVADLAAMRAAVARLGGDP
                     SRVNPRIPGDLVIDHSVIVDVFRSPQAFARNVEMEYERNGERYAFLRWAQQAFDRFSV
                     VPPGAGIVHQVNLEYLGTVVMTSDDVAYPDTLVGTDSHTTMIGGLGVLGWGVGGIEAE
                     AALVGEPVTLLTPEVVGFRLTGELQDGITATDFVLAVTEMLRRHGVVGKFVEFFGPGI
                     RTLTVPDRATISNMSPEYGATEGIFPVDDQTLAYLRGTGRDEAQIDLVERYTKEQGLF
                     HQPDDPQATYSELLELDLSSLEPSLAGPKRPQDRVGLGEVKAAFRAELAEQRAPALAG
                     GQARGESPAEHVEAEPEPEIKRVQVQMEEGAEELADGSIVISAITSCTNTSNPSVMVG
                     AGLLARNAVERGLTVSPTVKTSLAPGSPVVMDYLRNAGLVEPLEQLGFGLVGFGCTTC
                     IGNSGPLPDAVAEAIDEHGITGVAVLSGNRNFEGRIHPQVRMSFLGSPPLCVAYALAG
                     SADVDLTEDPLGLDRDGNPVYLRDIWPGIEEITRVVNEAMDPQAYRTRYGEIFSGDER
                     WLSLSVPEGDLYEWDPDSTYITEPPFVQDIGLEPEPVEDITGARVLVSVGETVTTDHI
                     SPAGSIPANSPAGEWLQERGVPRLGLHSYGARRGHHEVMVRGTFGNIRLRNRIAPGTE
                     GGVTIHLPSGEETSIYEAAIRYREEGTPLLVLAGHEYGAGSSRDWAAKGTQLLGIRAV
                     IAQSFERIHRSNLAQMGVLPLQFHDGESRESLGLDGTEVYDIEGVSDVTPGATLDVVA
                     RKEDGSEVRFQARCRLDTPTEVRYYRDGGILPAVVRELAKS"
     gene            6796..7362
                     /gene="mug"
                     /locus_tag="VF058_06445"
     CDS             6796..7362
                     /gene="mug"
                     /locus_tag="VF058_06445"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020548551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HEX6207985.1"
                     /translation="MTREDLGRRPTKEEVAGAAGRTVPDVIGPGLLVLFVGFNPGLYS
                     GAVGHHFARPGNRLWKALAGSRFTPRVLSPHEEEDLLELGLGITNLVERATAGAKELS
                     KEELREGAVRLRAKVERWGPRWVAFLGIGAYRTALERPKAVIGEQEERFGGAKVWVLP
                     NPSGLNAHYQPPDLIERFAELRAATRGS"
     gene            complement(7346..7780)
                     /locus_tag="VF058_06450"
     CDS             complement(7346..7780)
                     /locus_tag="VF058_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6207986.1"
                     /translation="MTVPPHVSLITLGAADVARATHFYEALGWRRSSASAEGEVSFFH
                     TRGAIVGVYDEGMLADDVGLGGERPSRFRGVTLAINVPSEAEVDAALEAARDAGAAVA
                     DAGRTPWGGYVGYFTDPDGHVWEVAHNPTFPLGEDGSITIPE"
BASE COUNT         1122 a         2735 c         2766 g         1192 t
ORIGIN      
        1 accacccgtt cgacgtatcg ccgcgcggcc tcgaagccgt gccgcccggc gcgagccgcc
       61 ccgagcgcgt cggcggcgac gcctcccacc tcgacgtcca ccggctcgcc cacctcccgc
      121 gccgtgtgga gcgtggggca acgctccggc tccaccccga tcacgcggac gtcgttgccg
      181 aaccaggtgc agacgccgcc gatcagcccc ccgcctccca ccgcgacgag caccgagtcg
      241 agatcgggcg cctgctccgc cagctcgagc ccgatcgtcc cctgccccgc cacgacctcg
      301 ggctggtcgt aggcgtgcac aacgagcgcg cccgtctcct cggcccggga acggctggcc
      361 tccaatgcct ccgcgtagta gccgggcacc acgtgcaccg tcgcgccgag ctctcggatc
      421 ccctcgatct tggtctccgg cgaggtgtcg gggatgaaga tctcggcggc cacccccagc
      481 cgcgcggctg cgaacgccac cccgagcccg tggttgccgc cgctcgccgc gatcactcct
      541 gccttcggga cctcgttcgc caggatccgg ttgaaggccc cgcggggctt gaacgagccc
      601 gtgtgctgga gctgctcgag cttgaggacg aggggatcgt cgatcccgaa cgacccggtg
      661 ccgggctcga agaccggggt ccgtcgcacg aacccgcgga tccgctcgtg ggccgcctcg
      721 atgtcggtgc gcgtgacctc gcggacgggg gcgtccgcgc gctccagcgg tcccttcctc
      781 gtctggctca ggcctgctcc ttcaggtagc ggatcaatcc gccggccagg aggatatccc
      841 gctcactggg cgagagggga gcgcgggcga tgtgttgccg gccctcggcc tcgaccgtga
      901 tctcccccca ggcgagttcc tcgcggaccc gaggtaggcg ccagacctgc ccctgctggg
      961 ccgtcttctg caggtcttcg tcgatcagca gggggaggat ccccacgttg atcaggttct
     1021 tccggtggat ccggtggatc ccggtggcga agacggcgcg cacgccgagc tcgagtggcg
     1081 cgagcgcggc gtgctcccgg ctcgacccct ggccgtagct gtcgcccgca acgatgaacc
     1141 cgcctcccca ctcccgcgct cgcgccacga acgtcggatc ttctttctgg agcgtgtact
     1201 cggcgatcct ggcgatgttc gaccgctcgc ccatgacgat caccccgtcg ggcgagagtg
     1261 acccggtcga gatgttgtcg ccgagcacga tcaggacgcg gccctcgagc gcaaccggaa
     1321 ggtcgggcgg gagcggcggc ggcacgatgt tcgggccccg ccagatctcg accttcgccc
     1381 gctcctcctc gggttgaggg gggatcagca tgcggtcgtc cacgaccatg gggggctcgt
     1441 cgatcttcgg agggtccatc cccaggtcgc gggggtcggt gatcacgccg cggagcgccg
     1501 tggcgccggc caccgccggc gacgccaggt acacgcggtc gtccacggtg cccgagcggc
     1561 ccgggaagtt gcggttcatc gtccggacgc tcggctggtc gctcggcggg gcctgcccca
     1621 tccccacgca gggcccgcac gcgggctcga gcagccgcgc acctgccgcg atgtagccgg
     1681 cgagcacgcc cgtccgcgag atcgtgtcga ggatctgccg ggagcccggg ctcaccgtca
     1741 tgtcgagcca ctcgtcgacg ccgccctcct tgacgatcgc ggccgggatc gccaggtcct
     1801 cgtagctgga gttgacgctg gagcccacgc agacctgggc caccttcgtg cccgccacct
     1861 cgcgcaccgg gacgacgttg tcggggttcg agggcaaggc gatcagcggc tcgagccggg
     1921 cgaggtcgat caccatctcc tcgtcgtagc tcgcgccatc gtcggcctgg agctctacga
     1981 agtcgtcggc ccgctcctgc gcggtcagcc agtccttcgt gtggcgatcg gaggggaaga
     2041 ggcccccggt cgcgccgagc tcgaggatca tgttgcagat cgtggcccgc tcggggaccg
     2101 agagcgtgtc caccccgggt cccgcgaact cgaacgcctt ccccacgccg cccttcaccg
     2161 agaggcgccg cagcatctcc aggatgacgt ccttggccga cacccatggc gagagctcgt
     2221 tctccagcac cacgcggacg accgaaggca tcgccagctc gaacggatgg ccggccatca
     2281 ccacggccgc gtccatcccg cccactccga gcgcgaacat ccccatcgct ccgccggtcg
     2341 tggtgtggga gtcggagccg atcatcgtct ggcccggcac gtcgaaccgc tcgaggtgca
     2401 cgtagtgcga gatcccgttc ccgggcctcg agaaccacgc gccgtatcgc atcgcgcacg
     2461 tctggaggaa gaagtggtcg tcggggttct tgtggtcgag ctggatcatg ttgtggtcca
     2521 cgtactggac cacggtggga gggtggacgc ggtcggcccc gagctgctcg aactccatcc
     2581 aggccatcgt cccggtcgcg tcctggagca ggacctgatc gatcgagaac cggatcggct
     2641 ggccgggcgt catctcgccg tccgccaggt gctgcccgag gatcttccgc gtcaggttct
     2701 ccgccacggc cgaaccacct ccgaggcgag cctaccccgc aggcggccac ggaaccgaac
     2761 tcccttcgcg catgggacga tgactgtgta actatcgatc gggagctgag atggaagcgt
     2821 tgatggcgac tgtccgcaca gtcgttgacc tgatcgttcg tggcgagttc gagactctcg
     2881 aatccatgac gcggggccgt cggctaaccg catcagagct tcgagcgagc gttgaggcct
     2941 acggacggaa gcttgctcgg ccccctgaag aagcctggga ggagatcgat gtcgtggaag
     3001 tcgagagcga gtctcggatc tacaacgtgc acatcccact gtggacggag gaggaagggc
     3061 ggtcagacct gaccctcgaa ttgaccctca cagagttcgc tccagggctg ttctcaattg
     3121 aggtagatgg catccacgtt ctgtaggagg ctctgtcccc tggagcacgt ggtcgacagg
     3181 ggcgtccgtc caggcgtgta gcatcccgag catgagcgtc gtggagcacc ttcgggaggc
     3241 ggcgccgggc gtcttggagg acgagccggt ggtcttcgcc tacctgttcg gatcgcaggc
     3301 caccggccag gcccatccgc ggagcgacgt ggatgtggcc gtcatgctcg atgagaccgt
     3361 gccggaggac gagcgcctgg agacctcgct ccgtctggcg cgacgtttgt cggaggcttc
     3421 tggactgggc aggatcgagg tcgtcctcct caacgacgct ccgcttcccc tcgtcggcag
     3481 ggcgatctcg acgcggcagg tcatctactc gaaggacgag ccagcgcggg tggagttcga
     3541 gagccggcgg caccgagagt tcctcgactt ccagatccac gcggagccgc tggcccgcgc
     3601 actcctccgg cgcgccgcgg gcgagcgctg atggtcgacg tcggccgggt gctcgcgctg
     3661 ctcgaaaggc tccaggcgga gacggacgag ctggccaagc tgagggactc cgaggcccta
     3721 ccaggagatg agaccgctct ggcggccgcc aagtatcgct tcatcgtagc gatcgggacc
     3781 tgcatcgacg tcgcggaaca cgtgatcgcc tccgaaggtt tgcgttctcc cgacagcttc
     3841 gccgaggcgt tcgccgtcct gggggatgtg agcttcatcg aggaggacct ggctgccgac
     3901 ctccaggaca tggcgcggtt ccggaacctc ctcgtccacg gctaccaacg agtggacgac
     3961 gcccgggtgg cggagatcct gcggacccga ctggacgact tcgagcgctt ccgtgccgcg
     4021 atcgcccgag tggtctccta gctcttcgcc agctcgcgga cgacggcggg gaggatgccg
     4081 ccgtcccggt agtaccggac ctcggtgggg gtgtcgagac ggcagcgggc ctggaaccgg
     4141 acctcgctcc cgtcctcctt ccgggccacg acgtccagcg tcgcgccggg ggtgacgtcg
     4201 gacacaccct cgatgtcgta gacctcggtc ccatcgaggc cgagcgactc gcgcgactcg
     4261 ccgtcgtgga actggagcgg caggacgccc atctgcgcca ggttcgatcg gtggatccgc
     4321 tcgaacgact gcgcgatcac cgcacggatc cccaggagct gcgtcccctt ggccgcccag
     4381 tcgcggctgg agccggctcc gtactcgtgg ccggcaagca cgagcagggg cgtgccctcc
     4441 tcacggtagc ggatggccgc ctcgtagatg ctcgtctcct cgccgctggg aaggtggatc
     4501 gtgacgcccc cctcggtgcc cggcgcgatc cggttgcgga gccggatgtt cccgaacgtc
     4561 ccgcggacca tcacctcgtg gtggccgcgg cgcgcgccgt aggagtggag ccccagccgc
     4621 ggtacgccgc gttcctggag ccactctccg gccggcgagt tcgcagggat cgagcccgcg
     4681 ggtgagatgt ggtcggtcgt caccgtctcg cccacgctca ccagcacgcg agcgccggtg
     4741 atgtcctcga ccggctcggg ctccagcccg atgtcctgta cgaagggcgg ctcggtgatg
     4801 tacgtggaat ccgggtccca ttcgtagagg tcgccctccg gcaccgacag cgacagccag
     4861 cgctcgtccc cgctgaagat ctcgccgtag cgcgtccggt acgcctgcgg gtccatcgcc
     4921 tcattgacga cccgggtgat ctcctcgatg ccgggccaga tgtcgcggag gtagacgggg
     4981 ttgccgtcgc ggtccaggcc gagggggtcc tcggtgaggt cgacgtcggc cgagcccgcc
     5041 agcgcgtacg ccacgcagag cgggggcgag cccaggaacg acatccggac ctgggggtgg
     5101 atgcgcccct cgaagttgcg gttgcccgag aggacggcca cgcccgtgat cccgtgctcg
     5161 tcgatcgcct cggccacggc gtcgggcagc ggtcccgagt tcccgatgca ggtcgtgcac
     5221 ccgaagccca cgagcccgaa gccgagctgc tccagcggct cgacgaggcc cgcgttccgc
     5281 aggtagtcca tgacgacggg agagcccggg gccagcgacg tcttcacggt ggggctcacc
     5341 gtgagacccc gctcgacggc gttccgagcc aggagccctg cccccaccat cacgctgggg
     5401 ttcgaggtgt tcgtgcacga ggtgatggcc gagatcacga tcgagccgtc ggcgagctcc
     5461 tcggctccct cctccatctg gacctggacc cgcttgatct cgggctcggg ctcggcctcc
     5521 acgtgctcgg cgggggactc gccgcgagcc tggccgccgg ccagggccgg cgctcgctgc
     5581 tccgcgagct ccgcccggaa ggcggccttg acctcgccca accccacgcg gtcctggggc
     5641 cgcttggggc ccgccagcga gggctccagc gacgagagat cgagctcgag cagttccgag
     5701 taggtggcct ggggatcgtc gggctggtgg aacagcccct gctccttcgt gtaccgctcc
     5761 acgagatcga tctgcgcctc gtctcgtccc gtgcctcgga ggtaggccag cgtctggtcg
     5821 tccacgggga agatgccctc ggtggcgccg tactcgggcg acatgttcga gatcgtggcg
     5881 cgatcgggga cggtcagcgt gcggatgccc ggcccgaaga actcgacgaa cttccccacg
     5941 acgccgtggc ggcgcagcat ctcggtgacg gccagcacga agtcggtggc ggtgatcccg
     6001 tcctgcagct cgccggtcag ccggaacccg acgacctcgg gcgtgaggag ggtgacgggc
     6061 tcgccgacga gggcggcctc ggcctcgatc ccgcccacgc cccacccgag cacgccgagc
     6121 ccgccgatca tcgtcgtgtg cgagtcggtg cccacgagcg tgtcgggata ggccacgtcg
     6181 tccgaggtca tcacgaccgt ccccaggtac tcgaggttca cctggtgaac gatccccgcc
     6241 cccggcggca ccacgctgaa gcggtcgaac gcctgttggg cccaacggag gaacgcgtag
     6301 cgctcgccgt tccgctcgta ctccatctcg acgttgcggg cgaaggcctg gggcgagcgg
     6361 aagacgtcca cgatcaccga gtggtcgatc accaggtcgc ccgggatgcg ggggttgacg
     6421 cgcgaggggt cgcccccgag ccgggcgacg gccgctcgca tggcggccag gtcggccacg
     6481 accggcacgc cggtgaagtc ctggaggacg acgcggccgg ggaagaaggg gatctcgcgg
     6541 cgggcggcct cgggccaccc cgccagggcc tcgacgtgct cggcctccgt tccctcgccc
     6601 tgcttccgca gcgcgttctc caggaagaca cggatcacgt acgggagccg ctcgaggacg
     6661 gtctcgtcgg cggcgcgggt gagcgggacg tatcggatgg tcccgcccga ggtctccagc
     6721 tgctcggtca tcgctctcct caagtcgctc accttcgagg ataccccggt cgcgtcagcc
     6781 ggtagcctgc cggggatgac gcgcgaggac ctggggcggc gccccaccaa ggaggaggtc
     6841 gcgggggcgg ccggcaggac ggtgccggac gtgatcgggc cgggcctctt ggtgctgttc
     6901 gtggggttca acccgggcct ctactcgggc gcggtcgggc accacttcgc caggccggga
     6961 aaccgtctct ggaaggcgct ggccggttcg cggttcacgc ctcgcgtgct gtcgccgcac
     7021 gaggaggaag acctgctcga gctggggctg gggatcacga acctggtgga gcgggccacg
     7081 gcgggagcga aggagctctc gaaggaggag ctgcgcgagg gcgcggtgcg gctgcgggcg
     7141 aaggtcgagc gctgggggcc gcgatgggtg gcgttcctcg ggatcggcgc ctaccggacg
     7201 gcgctggaga ggccgaaggc ggtgatcggg gagcaggagg agcgcttcgg cggggcgaag
     7261 gtctgggtgc tgccgaaccc cagcggcctc aacgcccact atcagccgcc ggacctgatc
     7321 gagcggttcg ccgagctccg agcggctact cggggatcgt gatcgagccg tcctccccga
     7381 gcgggaaggt ggggttgtgg gcgacctccc agacgtggcc gtcggggtcc gtgaagtagc
     7441 ccacgtaccc cccccacggc gtccgcccgg catcggcgac cgcggcgcct gcgtcccgcg
     7501 ccgcttccag cgccgcgtcc acctccgcct cgctcggcac gttgatggcc agggtgacgc
     7561 cccggaagcg cgagggccgc tccccaccca gcccgacgtc gtccgcgagc atcccctcgt
     7621 cgtagacgcc gacgatcgcc ccgcgggtgt ggaagaagga gacctctccc tcggcggagg
     7681 ccgaggagcg gcgccagccg agcgcctcgt agaagtgggt ggcgcgggca acgtccgcgg
     7741 ccccgagcgt gatgagcgag acgtgcgggg ggacggtcac gcggggaagc ctagcgggtg
     7801 ggggcggcgc gcgcg
//
LOCUS       DASUTW010000186         2949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1093900, whole genome shotgun sequence.
ACCESSION   DASUTW010000186 DASUTW010000000
VERSION     DASUTW010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093900"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VF058_06455"
     CDS             complement(<1..272)
                     /locus_tag="VF058_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX6207987.1"
                     /translation="MAIGDLAAEQFCYLTSIGRVTGRPHTIEIWFALDGRTLYLLSGG
                     GRASDWVRNLERTPDVTVRLGDRTFPGRARVVTDAEEDERARRLVH"
     gene            339..1820
                     /locus_tag="VF058_06460"
     CDS             339..1820
                     /locus_tag="VF058_06460"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6207988.1"
                     /translation="MACDDEDLGLPIKLGPCSNAEYDPEPLPQVLRETIRRTRASSER
                     HAGRLAISRREFLRSSMGAAAMLFALDACTRDRMRTVERRDPGGRYRIPDEAAEDAFL
                     AEETVEGEEFVFDVQGHLLEYDLDPATRWEPFWGDGFPQVACGEDDPRACFSMEHFME
                     EFFLRSDTSMVALAGLPIAPAGSPLSPEIMEEARRVAEGLCRDERVLLHALVLPNVGP
                     LAANLDAMEEAAGPYPIAAWKVFTHFPDVYERSGSGWRLDDGDPSLATVGAPFIRKAV
                     ELGVPRIAVHKGLSGGSALASPIDVGAAARAHPDVAFIVYHSGFEAGISEGPFTRRTA
                     GVGVNRLIASLKRNGIRPGSNVYAELGSTWWYVMRYPDQAAHVLGKLLKHVGEDNLLW
                     GTDSIWYGSPQDQIQALRSFHISEEYQERFDYPALTKRIKRKILGLNAADLYGIDPAA
                     VPCTFTRPELRRIRRELRRPSSTNGPSTPAEAQRVREHHQGMP"
     gene            complement(1846..>2949)
                     /locus_tag="VF058_06465"
     CDS             complement(1846..>2949)
                     /locus_tag="VF058_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6207989.1"
                     /translation="AHEDVERVADLLAAAASPVLVGGRGAVVAGAGPAIEALGDRVGA
                     LLATSANGHGLFAGNPWALGISGGFASPTAAALIAEADVLVSFGASLNMWTTRHGELV
                     GPGTRVVQVDRDADELGAHRPIDLGVVGDARLTAEGVIVELDRRGHPAAGRRTDEIAE
                     RIASGAWGDESHEDASVDGALDPRTFSKALEEALPPERVVAVDSGHFMGWPPMYLSVP
                     DPAGFVFTQSFQSIGLGLSSAIGAAVAQPDRLAVACLGDGGAMMAAGEFETIVRLGLP
                     MLVVVYNDAAYGAEVHHFGPQGHPVDLVRYPDPDLAGLARGLGAEAATVRSLDDMSVV
                     DAWLERRDGPLVLDVKIVPDVVAEWLEEAFRSH"
BASE COUNT          448 a         1101 c         1008 g          392 t
ORIGIN      
        1 tcgtggacga ggcggcgagc tcgctcgtcc tcctcggcgt ccgtgaccac ccgggcgcgg
       61 ccggggaagg tcctgtcccc caaccgaacc gtcacgtcgg gcgttcgctc caggttccgc
      121 acccaatcgg aggcccgccc gccccccgac aggaggtaga gcgtcctccc gtcgagcgcg
      181 aaccagatct cgatggtgtg cgggcggccc gtgacgcgtc cgatggaggt gaggtagcag
      241 aactgctcgg ccgcgagatc cccgatcgcc atctcgagcc caccttagct gcgtgaacca
      301 ccccgcaccc tggagcgtct acctctcggg ggatcgccat ggcgtgtgac gacgaggacc
      361 tcgggctgcc gatcaagctg ggtccgtgct cgaacgccga gtacgatccg gagccgctgc
      421 cccaggtcct gcgagagacg atcagacgca cccgggcgtc ctcggagcga cacgccggtc
      481 gcctggcgat cagccgtcgc gagttcctgc gctcctcgat gggtgcggcc gcgatgctct
      541 tcgcgctcga tgcgtgcacc cgcgatcgga tgcgaacggt cgagcggcgc gatccgggag
      601 gccgctaccg catccccgac gaagccgccg aggacgcctt cctcgcggag gaaaccgtcg
      661 agggcgagga gttcgtcttc gacgtccagg gccacctgct cgagtacgac ctcgaccccg
      721 ccacgcgctg ggagccgttc tggggcgacg gcttccccca ggtagcctgc ggcgaggacg
      781 acccgagagc ctgcttctcc atggagcact tcatggagga gttcttcctc aggagcgaca
      841 cgagcatggt ggcgctcgcc ggcctcccca tcgccccggc cgggagcccc ctctccccgg
      901 agatcatgga agaggcccgg cgggtcgccg agggcctctg ccgtgacgag cgggtcctcc
      961 tccacgcgct ggtgcttccg aacgtcgggc cgctcgccgc caacctcgac gcgatggagg
     1021 aggcagcggg gccctacccc atcgccgcgt ggaaggtgtt cacgcacttc ccggacgtct
     1081 acgagcgctc ggggagcggg tggcgcctcg acgacggcga tccgagcctg gcgacggtgg
     1141 gggccccgtt catcaggaag gccgtcgagc tcggcgtgcc ccggatcgcc gtgcacaagg
     1201 gtctcagcgg cggaagcgcc ctggcctcgc cgatcgacgt gggggcggcg gcccgggccc
     1261 atcccgacgt cgccttcatc gtgtaccact cggggttcga ggccgggatc tctgaaggcc
     1321 ccttcacccg caggacggcg ggcgtgggcg tcaacaggct gatcgcttcc ctcaagcgaa
     1381 acgggatccg gcccggcagc aacgtgtacg cggagctcgg gtcgacctgg tggtacgtga
     1441 tgcgctaccc ggaccaggcc gcccacgtgc tgggcaagct tctgaagcac gtcggcgagg
     1501 acaacctcct gtggggcacg gattcgatct ggtacggctc cccacaggat cagatccagg
     1561 ccttgcgctc gttccacatc agcgaggagt accaggagcg cttcgactat cccgctctca
     1621 ccaagaggat caagcgcaag atcctcgggc tgaacgccgc cgatctctac gggatcgacc
     1681 ccgccgccgt tccctgcacg ttcacgagac ccgagctgcg caggatccgg cgagagctgc
     1741 ggcggccaag cagcaccaac ggacccagca cccccgccga ggcccaacgg gtccgggagc
     1801 atcaccaggg gatgccctga ccgccccggt ccctcgccgt ggcggtcagt gcgagcggaa
     1861 ggcctcctcg agccactccg ccacgacgtc ggggacgatc ttgacgtcga gcacgagcgg
     1921 accgtcgcgc cgctcgagcc atgcgtcgac cacgctcatg tcgtcgaggg accgaacggt
     1981 cgcggcctcg gctcccagtc cccgggcgag cccggccaga tctggatccg ggtagcggac
     2041 gaggtccacc gggtggccct gcgggccgaa gtggtgcacc tccgccccgt acgccgcgtc
     2101 gttgtagacg accacgagca tcggaagccc gaggcgaacg atcgtctcga actccccggc
     2161 cgccatcatc gcaccgccgt ccccgaggca ggccacggcg aggcggtccg gctgggcgac
     2221 cgccgccccg atcgcggacg agagccccag cccgatcgac tggaacgact gcgtgaagac
     2281 gaagccagcc gggtccggga ccgagaggta catcgggggc cagcccatga agtgcccgga
     2341 gtccaccgcg acgacgcgct cgggcgggag cgcctcctcc agggccttgg agaacgtccg
     2401 cgggtcgagc gcgccgtcca ccgaggcgtc ctcgtgcgat tcgtcccccc acgccccgga
     2461 cgcgatcctt tcagcgatct catcggtgcg gcgccccgcc gccggatgac cacggcggtc
     2521 gagctccacg atcaccccct cggccgtcag ccgcgcgtcg ccgacgacgc ccaggtcgat
     2581 cggccggtgc gcccccagct cgtccgcgtc gcggtcgacc tggacgaccc tcgtcccggg
     2641 cccgaccagc tctccgtggc gcgtcgtcca catgttgagg gaggcgccga acgacacgag
     2701 gacgtccgcc tcggcgatca gcgcggcggc cgtcggggag gcgaagccgc ccgagatccc
     2761 gagcgcccac gggttccccg cgaagagccc gtggccgttg gcggacgtcg ccagcagcgc
     2821 cccgacccgg tcgccgagcg cctcgatcgc gggaccggct ccggccacca ccgcgccgcg
     2881 cccgcccacg aggacgggcg aggccgcggc ggccagcagg tcggcgaccc gctccacgtc
     2941 ctcgtgggc
//
LOCUS       DASUTW010000187         6385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_58435, whole genome shotgun sequence.
ACCESSION   DASUTW010000187 DASUTW010000000
VERSION     DASUTW010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58435"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VF058_06470"
     CDS             complement(<1..294)
                     /locus_tag="VF058_06470"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:NF014769.4"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HEX6207990.1"
                     /translation="MIELSREVVANAVLAALREDLGAEGDVTSAAVIPEVQTARARIV
                     SRSRGVLAGIPVAKECFSRVNTRLRPLKEDGDRIVPGDEVAVVGGALRAILAAE"
     gene            complement(291..1133)
                     /gene="panC"
                     /locus_tag="VF058_06475"
     CDS             complement(291..1133)
                     /gene="panC"
                     /locus_tag="VF058_06475"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX6207991.1"
                     /translation="MIRRREPLRDAADDARLEGRTVGFVPTMGFLHEGHLSLVRKARR
                     EADLVVASIFVNPLQFGPGEDLDVYPRDEARDLELLEEAKADLAFVPTVEEMYPDGAP
                     DVTVDPGSLGDVLEGASRPGHFRGVCTVVAKLCGIVGPCRAYFGEKDAQQLAVVRRMV
                     EDLDLPVSVLSGETVREPDGLAMSSRNVYLSPEERETARAILRALAAAEALVEGGERD
                     AHVVRAEMAKHVGDEPLAELDYVAVVDDATFEEVDRLERPARALVAARFGRARLIDNV
                     RLAP"
     gene            complement(1130..1996)
                     /locus_tag="VF058_06480"
     CDS             complement(1130..1996)
                     /locus_tag="VF058_06480"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEX6207992.1"
                     /translation="MKVAVVGAGRVGTAMGVLLSRAGHTVVAASGRGVGSRERAERHL
                     PEAAFVGSVEAARRGEVVLLAVPDDRILDVCGTLANRGGLTSGPAVVHLSGANPLQDL
                     APAAEVGASILSVHPLQTAPSVEAAIERFPGSGFAVTARDEDSFLLGERLAADAGGRP
                     FRLLDEHKGLYHAAAVFASNYLVVVEGLADDLFELAGIPDPRPLFGPLAEGTLANVME
                     QGAARALTGPVARGDAGTVRRNLEALRDRAPSCVAPYAELARAAVRIASEAGRLNDRD
                     RVGIEEVLEEWT"
     gene            complement(2060..2638)
                     /locus_tag="VF058_06485"
     CDS             complement(2060..2638)
                     /locus_tag="VF058_06485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6207993.1"
                     /translation="MSLDVRPVTPDRWDDLVALFGVDRGAYSGCWCMWWRQTAKGYDR
                     DHGAPNREAMRAIVREGREPGLLAYRRGEPVGWVSLGPRDDFGRLDRSRVLGRVDEEE
                     GVWSIVCFYIHRRHRRDGVAKALLAAAVARAAERGARWVEAYPVDLGAAAAKKASAEL
                     FTGTLVMFEEAGFREVARRGAARPIVRAPVAR"
     gene            complement(2635..3180)
                     /locus_tag="VF058_06490"
     CDS             complement(2635..3180)
                     /locus_tag="VF058_06490"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEX6207994.1"
                     /translation="MSTDLELVAAFVNTLDVGSGRDALESAEALRGWLVQRGLLGGDH
                     PVTEADLGRALAVREAIRGLAERNNPGAGTPPDAEPLETLNRLAGGSRLVLSFGPEGD
                     ARLESVDEGVPGALGRILAAVFLAMTDGRWERFKACARDSCRWVYVDRSRNRSRTWCS
                     MDVCGNREKARAFRERRKAKR"
     gene            3316..3480
                     /locus_tag="VF058_06495"
     CDS             3316..3480
                     /locus_tag="VF058_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6207995.1"
                     /translation="MQGHTIWELSRMHAADLLLDAGPVRRRPRRRRVRRAVGTGMVRT
                     GYRLMGARAS"
     gene            3477..3794
                     /locus_tag="VF058_06500"
     CDS             3477..3794
                     /locus_tag="VF058_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX6207996.1"
                     /translation="MIARIWTGQTRAEDAEAYAGYLARTGFPDYRGTPGNRGVLGLRR
                     IDGDRATFTLITLWDSIDAIRAFAGEDPERARYYDEDDRFLLSFPERATHYEVIEGPQ
                     TAR"
     gene            3791..4447
                     /locus_tag="VF058_06505"
     CDS             3791..4447
                     /locus_tag="VF058_06505"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX6207997.1"
                     /translation="MKAMEQGYRHGARAILPLIVPVIGFGVTFGVLAREAGMGILAPV
                     VMSATTFAGSAQFAASSILGVGGGAVAAIVAALLLNARYTPIGLTVAGAIEGPSWLRF
                     LRSQLIVDESWAVATTPEGVDGRRLIGAGLVLYATWVTSTLVGALGARILGDPATLGL
                     DAAFPAVFVALLVPQLAGRRPRLAAGLGALIALALLPWAPAGVPVIAATAAILVGWSR
                     "
     gene            4444..4746
                     /locus_tag="VF058_06510"
     CDS             4444..4746
                     /locus_tag="VF058_06510"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX6207998.1"
                     /translation="MKVWVVVAAAGAGTAILKAAGPLLLHGRPMSPRAQRMLSLLAPA
                     LLSALILVQVLGVEDGIRFDERLLGVGTAIVAAALRAPIWVVVGVAALVTALARLV"
     gene            4752..5120
                     /locus_tag="VF058_06515"
     CDS             4752..5120
                     /locus_tag="VF058_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018779814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6207999.1"
                     /translation="MVERDDVTRWIEGYERAWASNERGDIEALFTEDARYFTAPYRKP
                     WEGREAIVDGWLHDRDEPGQWRFASDVLAIANDLAFVQGRTEYLVEPPRPYSNLFVIR
                     FAGDGRASEFTEWFMPIEDA"
     gene            complement(5117..6160)
                     /locus_tag="VF058_06520"
     CDS             complement(5117..6160)
                     /locus_tag="VF058_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6208000.1"
                     /translation="MGYARIVVGTDGSETAGRAVDRAARLSKDLGSTLILVTASAPIG
                     MIDTVAEEIAVAARDRVRAKGIEAEAVVQEGDPAEVLTAVAAAEDAGLVVVGDQGLGR
                     ARRVRLGGVAERVAVMAPCDVLIVETQNPREEPAEGPLYRRLLVGTDGSPTATAAVQR
                     AYDFGMMIGVGVLVATVTDDDVLAAIHLEDARRIKPRALGVEAKVLRGEPAKALVKEA
                     DREGCELIVVGNRGLTGARRVLLGSIPSRVAHRADRDVLIARTVGRTIKDLRPGQGAV
                     VDAGSGKMVAAFLGEDGGVLALDPRCTHMGCTVRFNQSERTWDCPCHGSRYDLEGQVL
                     QGPAKKPLPRTTL"
     gene            complement(6160..>6385)
                     /locus_tag="VF058_06525"
     CDS             complement(6160..>6385)
                     /locus_tag="VF058_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208001.1"
                     /translation="SIGILVAIIVTMYAIAGPYYRRVRTIVQAMARGSEAVTPQQLDE
                     ILRAPSALWNAIFGFAALLAIPYLMVFKPF"
BASE COUNT          856 a         2485 c         2172 g          872 t
ORIGIN      
        1 ctccgcagcg aggatcgcgc ggagcgcacc gcccaccacc gccacctcgt cgcccgggac
       61 gatccgatcg ccgtcctcct tcagggggcg gagccgggtg ttcacccgcg agaagcactc
      121 cttcgccacc gggatcccgg ccagcacccc ccgcgatctc gagacgatcc gggcccgagc
      181 cgtctggacc tccgggatca cggcggccga cgtcacgtcc ccttcggcgc cgaggtcctc
      241 ccggagcgcg gccaggaccg cgttcgccac cacctcccgc gacagctcga tcacggcgcc
      301 agcctcacgt tgtcgatgag gcgggcccgg ccgaacctcg ccgccacgag cgcccgcgcg
      361 gggcgctcca ggcgatcgac ctcctcgaac gtcgcgtcgt ccacgaccgc cacgtagtcc
      421 agctcggcca gaggctcgtc ccccacgtgc ttcgccatct cggcccgcac cacgtgcgcg
      481 tcccgctccc cgccctcgac cagggcctcc gcggcggcca gggcccggag gatcgccctc
      541 gccgtctccc gctcctccgg cgagaggtag acgttccgag aggacatcgc caggccgtcg
      601 ggctcccgca cggtctcccc cgacagcacg gagaccggca gatcgaggtc ctcgaccatc
      661 cgccgcacca ccgcgagctg ctgggcgtcc ttctcgccga agtaggctcg gcacggcccc
      721 acgatcccgc agagcttcgc cacgaccgtg cagacccccc ggaagtggcc gggccgggag
      781 gctccctcca ggacgtcccc caggctcccc gggtcgaccg tgacgtccgg cgctccgtcc
      841 gggtacatct cctcgaccgt cgggacgaag gcgaggtcgg ccttcgcctc ctcgaggagc
      901 tcgaggtccc tggcctcgtc ccgggggtag acgtccaggt cctcgcccgg gccgaactgg
      961 agcggattca cgaagatcga ggccaccacc aggtcggcct cgcggcgggc cttccggacg
     1021 agcgacaggt gcccctcgtg gaggaacccc atcgtgggca cgaacccgac ggtccggcct
     1081 tcgagccggg cgtcgtccgc cgcgtcgcgg agcggctccc ggcgccggat cacgtccact
     1141 cctcgagcac ctcctcgatc ccgacccggt cccggtcgtt cagccggccc gcctcgctcg
     1201 cgatgcgcac cgcggcccgg gccagctcgg cgtagggagc cacgcacgag ggcgcccggt
     1261 cgcgaagggc ctcgaggttc ctccgaacgg tccccgcgtc acctcgggcc accgggcccg
     1321 tgagcgcgcg ggcggcgccc tgctccatga cgttcgccag cgtgccctcg gccaggggcc
     1381 cgaacagcgg cctgggatcg gggatccccg ccagctcgaa caggtcgtcg gccaacccct
     1441 ccacgaccac caggtagttc gaggcgaaca ccgccgccgc gtggtagagc cccttgtgct
     1501 cgtccaggag ccggaagggc cttccgccgg cgtcggcggc cagccgctct cccagcaaga
     1561 acgagtcctc gtcgcgcgcc gtcacggcga agccgctccc ggggaagcgc tcgatcgcgg
     1621 cctccacgct gggagccgtc tggagcggat ggaccgagag gatcgaggcc ccgacctccg
     1681 ccgccggggc gaggtcctgg agcgggttcg ctccggagag atggacgacc gccggccccg
     1741 acgtcaggcc cccacgattg gccagggtgc cgcacacgtc gaggatccgg tcgtcgggga
     1801 cggcgaggag caccacctct cccctccggg cggcctcgac ggagccgacg aaggccgcct
     1861 cgggcaggtg cctctcggcc cgctctcgcg acccgacccc gcggccggag gcggccacga
     1921 ccgtatgccc cgcgcgggag aggagcaccc ccatcgcggt cccgaccctg cccgccccga
     1981 cgacggccac cttcacgggg tcgcgtgggt cccgagtgga cgcgcgcgga cgatcgtgct
     2041 tcgacgcgaa gtatagaggt cagcgggcga cgggggcccg gacgatgggc cgcgccgccc
     2101 cgcgccgggc cacctctcgg aagccggcct cctcgaacat caccagggtg ccggtgaaga
     2161 gctccgccga ggccttcttc gcggcggcgg cccccaggtc caccgggtag gcctccaccc
     2221 atcgggcccc ccgctccgcg gcccgggcca cggcggccgc caggagggcc ttggccaccc
     2281 catctctccg gtgccggcgg tggatgtaga agcagacgat cgaccagacc ccctcttcct
     2341 cgtccacgcg tcccaggacg cgggagcggt ccagccgccc gaagtcgtcc cgcggcccga
     2401 gcgacaccca tcccacgggc tcgccccgcc gatacgcgag gagcccgggc tccctcccct
     2461 cccggacgat ggcgcgcatc gcttcgcggt tcggcgcgcc gtggtcgcgg tcgtacccct
     2521 tcgcggtctg ccgccaccac atgcaccagc agcccgagta cgcgccgcgg tcgacgccga
     2581 acaacgccac caggtcgtcc cagcggtcgg gggtgaccgg ccggacgtcg aggctcaccg
     2641 tttcgccttc ctccgctccc ggaaggcccg ggccttctct cggttgccgc agacgtccat
     2701 cgagcaccag gtccgcgagc ggttcctgga gcggtcgacg taaacccagc gacacgagtc
     2761 gcgggcgcag gccttgaagc gctcccatcg gccgtcggtc atggcgagga agacggcggc
     2821 caggatccgg cccagggccc cgggcacccc ttcgtccacc gactcgaggc gcgcgtcccc
     2881 ctccgggccg aacgagagga ccagcctcga cccgccggcc agccggttca gggtctccag
     2941 cggctccgcg tccgggggcg tcccggcgcc cgggttgttc cgctcggcga ggccgcggat
     3001 cgcctcccgg acggccagag ccctccccag gtcggcttcg gtgaccggat gatcgccgcc
     3061 cagcaggccg cgctggacca gccatcccct gagggcctcc gcgctctcca gcgcgtcgcg
     3121 gccggacccc acgtccagcg tgttcacgaa cgcggccacg agctcgaggt ccgtcgacat
     3181 ctcaaccacc gtatccctct cgttggttat gaagcaagcg atcagctctc gcccccctaa
     3241 ccttcgtctg cgcttgacag gtgatgcgca accagtctac catgatgacc ggttacgata
     3301 gcagaggagg cggagatgca gggtcacacg atctgggagc tctcccggat gcacgccgcc
     3361 gacctgctcc tcgacgccgg gcccgtgcgg cgccggcccc ggcggcggag agtcaggcgg
     3421 gccgtgggga ccggcatggt gcggacgggg tatcggctga tgggagcgcg ggcctcgtga
     3481 tcgcccgcat ctggaccggc cagacgcggg ccgaggacgc cgaggcctac gcgggctacc
     3541 tggcccggac cgggttcccc gactaccggg gcactccggg gaaccgcggc gtcctcggcc
     3601 tgcgtcggat cgacggcgac cgggcgacct tcacgctcat caccctctgg gactcgatcg
     3661 acgcgatccg agcgttcgcc ggggaggacc ccgagcgcgc gcgctactac gacgaggacg
     3721 accggttcct gctctccttc cccgaacggg ccacgcacta cgaagtcatc gagggtcccc
     3781 agacggcaag atgaaggcca tggagcaggg gtaccggcac ggcgctcgcg ccatcctgcc
     3841 tctcatcgtc cccgtcatcg ggttcggcgt gaccttcggc gtcctggccc gggaggcggg
     3901 catggggatc ctggccccgg tcgtgatgtc cgccacgacc ttcgcgggat cggcgcagtt
     3961 cgcggcgtcc tcgatcctcg gcgtcggggg aggcgccgtg gcggccatcg tcgccgcgct
     4021 cctcctcaac gcgcgctaca cgccgatcgg gctcaccgtg gcgggggcga tcgaggggcc
     4081 gagctggctc cgcttcctcc gatcccagct gatcgtcgac gagtcctggg ccgtcgccac
     4141 cacccccgag ggcgtggacg gcaggcggct gatcggagcc gggctcgtcc tctacgcgac
     4201 ctgggtgacc tcgaccctcg tgggcgccct cggcgcgagg atcctggggg accccgcgac
     4261 gctcggcctc gatgccgcct tccccgccgt gttcgtggcc ctgctggtcc cccagctcgc
     4321 ggggcgtcgc ccacggctcg cagcgggcct gggcgcgctg atcgccctgg ccctgctccc
     4381 gtgggcgcct gcgggcgttc cggtgatcgc cgccaccgcg gcgatcctcg tggggtggtc
     4441 ccgatgaagg tctgggtcgt cgtggccgcg gcgggggcgg ggaccgcgat cctcaaggcg
     4501 gccggacccc tgctcctcca cggccgcccg atgtcgccga gggcccagcg gatgctctcc
     4561 ctgctcgccc ccgccctcct ctcggctctg atcctggtcc aggtcctcgg ggtcgaggac
     4621 gggatccggt tcgacgagcg cctgctcggg gtgggcaccg cgatcgtggc ggccgcgctc
     4681 cgggcgccga tctgggtcgt cgtcggggtg gcggccctcg tgaccgcgct ggctcgacta
     4741 gtctgaggag gatggtcgag cgagacgacg tgacccgttg gatcgagggc tacgagcggg
     4801 cctgggcctc caacgagcgc ggggacatcg aggcgctgtt caccgaggat gcgaggtact
     4861 tcaccgcgcc ctatcgcaag ccgtgggagg ggcgggaggc gatcgtcgac ggctggctcc
     4921 acgaccgcga cgagcccggg cagtggcgct tcgcgtccga cgtgctggcg atcgccaacg
     4981 acctggcctt cgtccaggga cggaccgagt acctcgtcga gcccccccgc ccgtactcga
     5041 acctgttcgt catccgcttc gcaggggatg ggcgcgcctc cgagttcacg gagtggttca
     5101 tgccgatcga ggacgcctag agggtcgtcc tcgggagcgg cttcttggcc ggcccctgca
     5161 ggacctgccc ttccaggtcg tatcgcgagc cgtggcacgg acagtcccac gtccgctccg
     5221 actgattgaa ccggaccgtg caccccatgt gggtgcagcg ggggtccagg gccaggaccc
     5281 ccccgtcctc ccccaggaat gcggcgacca tcttcccgct cccggcgtcc acgaccgcgc
     5341 cctgccccgg cctgaggtcc ttgatggtcc gccccacggt ccgggcgatc agcacgtcgc
     5401 ggtcggcgcg gtgcgcgacg cggctgggga tcgatccgag caggaccctg cgggccccgg
     5461 tcagcccacg gttcccgacc acgatgagct cgcacccctc ccgatcggcc tccttcacca
     5521 gggccttggc cggctcgccg cgcagcacct tcgcctccac ccccagagcc cgaggcttga
     5581 tcctccgcgc gtcctcgagg tggatcgcgg ccaggacgtc gtcgtcggtc accgtggcca
     5641 cgagcacccc gaccccgatc atcatcccga agtcgtaggc tcgctggacc gcggccgtgg
     5701 ccgtgggcga gccgtcggtt cccacgagga gacggcggta gaggggcccc tccgccggct
     5761 cctcccgggg gttctgcgtc tccacgatga ggacgtcgca gggcgccatc accgccacac
     5821 gctccgcgac gccccccagc ctgacccgac gggccctccc caggccctgg tcccccacca
     5881 cgacgagccc ggcatcctct gccgcggcca ccgcggtgag gacctcggcc ggatcccctt
     5941 cctgcacgac ggcctcagcc tcgatcccct tggcccgcac ccggtcgcga gccgcgacgg
     6001 cgatctcctc ggccaccgtg tcgatcatcc cgatcggggc ggaggccgtg accaggatca
     6061 aggtgctccc caggtccttg gacagccggg ccgcccggtc cacggcacgt ccggcggtct
     6121 cggaaccgtc ggtgccgacg acgatcctgg cgtagcccat cagaacggct tgaagaccat
     6181 caggtagggg atggcgagca acgccgcgaa cccgaagatc gcgttccaga gcgcggacgg
     6241 agcccggagg atctcgtcga gctgctgagg cgtcacggcc tcggagccgc gggccatggc
     6301 ctggacgatc gtcctcacgc gccggtagta ggggccggcg atcgcgtaca tcgtcacgat
     6361 gatagccacg aggatcccga tcgag
//
LOCUS       DASUTW010000188         2245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1094582, whole genome shotgun sequence.
ACCESSION   DASUTW010000188 DASUTW010000000
VERSION     DASUTW010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094582"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..472)
                     /locus_tag="VF058_06530"
     CDS             complement(50..472)
                     /locus_tag="VF058_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016881804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX6208002.1"
                     /translation="MLMRWDPFGELDRLTRWAGDTVRRPAVAMDAFRRGERFVMTFDV
                     PGIDPDTIDLTVQDNVLTLRAERRTTLDEYDQLLVHERPVGSYTRQVFLGENLDTEKL
                     EADYEQGVLTVTVPVAEAARPRKIEIGSKSKAIDAGAS"
     gene            complement(606..917)
                     /locus_tag="VF058_06535"
     CDS             complement(606..917)
                     /locus_tag="VF058_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208003.1"
                     /translation="MNDFAVRLIPYALSLAEQDPGELVVRVDLDGVGGGVWLQSTRPG
                     PPPPEDAAPHVIVGGRGPWLALVASGRVDPNVAMYEGVLNVGGDLAAGETILRSLRAY
                     P"
     gene            complement(914..1432)
                     /locus_tag="VF058_06540"
     CDS             complement(914..1432)
                     /locus_tag="VF058_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208004.1"
                     /translation="MVLDRHWLLGVARREREALGRTIQYTDPAAWENVGPWQGRPIIE
                     ILYHLAATEIAAAEIWGGEAPAELDEYRKAAGEDASPDGFTQWALARRAEQSAVQTAR
                     EWGRAADHLLDRATATSDEDWRERMLPWFEGDLRAGYFLQYRVAQWCSTARTSSTAAA
                     RRCGGSTTRRSW"
     gene            1492..>2245
                     /locus_tag="VF058_06545"
     CDS             1492..>2245
                     /locus_tag="VF058_06545"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX6208005.1"
                     /translation="MRVLMSPDKFKGTLTAQAAAEALATGWRRSRPRDDVVELPVADG
                     GEGTLSTLVAALGGEVRRAKVTGPLGDPVEAEFGLVETPEGREAIVEMARASGLELVS
                     EARRDPRRATTRGTGELILEAVEAGATRILVCIGGSATNDAGAGMAQAVGVRLYDEAG
                     EELPPGAEALDRLARIDLGGLDPRVAKASVVVACDVDNPLTGPRGASAVYGPQKGASP
                     GDVAFLDRCLSRFSAVAGRDLGLDLRGVEGAGA"
BASE COUNT          307 a          785 c          844 g          309 t
ORIGIN      
        1 gcgcgcggcg agttcctcgc cgcgcgcccc gccccgaaga ccgtcgccct tacgaggcgc
       61 cggcgtcgat cgccttcgac ttcgagccga tctcgatctt ccgcggcctg gcggcttccg
      121 cgaccggcac ggtgaccgtc aggacgccct gctcgtagtc ggcctcgagc ttctctgtgt
      181 cgaggttctc gcccaggaag acctgcctcg tgtaggagcc cacggggcgc tcgtggacca
      241 ggagctggtc gtactcgtcg agcgtggtcc tgcgctccgc gcgcagcgtg aggacgttgt
      301 cctgcaccgt caggtcgatg gtgtcggggt cgatgccggg gacgtcgaac gtcatgacga
      361 acctctcccc gcgcctgaag gcatccatgg ccaccgccgg gcgccggacc gtgtcaccgg
      421 cccagcgggt caggcgatcg agctcaccga acggatccca ccgcatcagc atggctccac
      481 cacctcctct caggacacct gattggagcc ttgttccgtt cgtagtatag aaacctgagc
      541 gcaaggctgt caacccctgg cccgccacgg aaccatcgca ggagcacgac ggggcggcaa
      601 gtccctcagg gataggctcg caagcttcgg aggatcgtct cgcccgccgc gaggtcgccc
      661 cccacgttga ggaccccctc atacatcgcc acgttcgggt ccacccgtcc gctggcgacc
      721 agggcgagcc agggccccct cccaccgacg atcacgtggg gcgccgcgtc ctcgggaggg
      781 ggcgggccgg ggcgggtgga ctggagccag acgcctccgc ccacgccgtc gaggtcgacc
      841 cggacgacca actcgcccgg gtcctgctcc gccagcgaga gcgcgtacgg gatcagccgc
      901 accgcgaagt cgttcaccac gaacgtcggg tcgtgctccc gccgcagcgc ctggccgctg
      961 ccgtcgagga tgtcctggcc gtggagcacc actgcgccac gcggtactgg aggaagtagc
     1021 cggcccggag gtccccctcg aaccagggga gcatgcgctc ccgccagtcc tcgtccgagg
     1081 tcgcggtggc gcggtcgagc aggtggtcgg cggcccgccc ccactcccgc gccgtctgga
     1141 cggccgactg ctccgcccgt cgggcgagag cccactgggt gaagccgtcg gggctcgcat
     1201 cctctcccgc cgccttccgg tactcatcca gctcggcggg ggcctcgccg ccccagatct
     1261 cggcggccgc gatctcggtc gcggcgaggt ggtagaggat ctcgatgatg ggccgcccct
     1321 gccacgggcc gacgttctcc caggcagcgg gatcggtgta ctggatcgtc ctgcccaggg
     1381 cctcgcgctc gcggcgggcc acccccagga gccagtgtcg gtcgagcacc atctcgcgcg
     1441 gaatcataca ggcgtacgga ggccgcgccg aaatcccgct aggctcgagc cgtgcgcgtc
     1501 ctgatgagcc cggacaagtt caagggaacg ctgacggccc aggccgcggc cgaggcgctg
     1561 gccaccgggt ggcggcgctc gcggccgcgc gacgacgtcg tggagctgcc ggtcgccgac
     1621 ggcggcgagg gcacactctc cacgctcgtc gcggcgctgg gaggggaggt tcggagggcc
     1681 aaggtgaccg ggccgctggg ggatccggtg gaggcagagt tcggtctcgt ggagaccccg
     1741 gagggcaggg aggcgatcgt ggagatggcc cgggcctcgg gtctcgagct ggtgtcggag
     1801 gccaggcggg acccgcggcg ggccacgacg cgaggcacgg gagagctgat cctcgaggcg
     1861 gtggaggcgg gggccaccag gatcctcgtc tgcatcgggg ggagcgcgac gaacgacgcc
     1921 ggggcgggga tggcccaggc cgtgggggtg cgcctctacg acgaggcggg ggaggagctc
     1981 cctccggggg ccgaggctct cgaccggctg gccaggatcg atctgggggg gctcgatccc
     2041 cgggtggcga aggcctcggt cgtcgtggcc tgcgacgtgg acaacccgct gacggggccg
     2101 cgcggcgcga gcgccgtgta cgggccgcag aagggagcct cgccggggga cgtggcgttc
     2161 ctcgaccggt gcctgagcag gttctcggcc gtggcgggga gagacctcgg cctcgacctg
     2221 cggggcgtgg agggggccgg ggcgg
//
LOCUS       DASUTW010000189         4434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1485054, whole genome shotgun sequence.
ACCESSION   DASUTW010000189 DASUTW010000000
VERSION     DASUTW010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485054"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VF058_06550"
     CDS             <1..220
                     /locus_tag="VF058_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003014486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208006.1"
                     /translation="SLGTDGFGRSDTRPALRRFYEVDAEHVVVATLAALAEMGEVKPE
                     QVAEAIERYDIDPERGAPFHPDAGTSLA"
     gene            234..419
                     /locus_tag="VF058_06555"
     CDS             234..419
                     /locus_tag="VF058_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208007.1"
                     /translation="MRKGHPRVLRPLLFLIVKAYQRAARSGEEAVLRPCPYCTTDIPV
                     AASRCPSCTSQAEPLAT"
     gene            469..2241
                     /gene="thrS"
                     /locus_tag="VF058_06560"
     CDS             469..2241
                     /gene="thrS"
                     /locus_tag="VF058_06560"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX6208008.1"
                     /translation="MADADRLEVLRHSTAHVMAQAVCDLFEGAKYAIGPPIEDGFYYD
                     FDLPETLTPEDLERIEARMREIIGEDQPFVREELSRDEALERFAGQPYKIEIIAGVEE
                     EEGAGAEGVSVYRNDGWADLCRGPHVGSTAELGAFKLMSVAGAYWRGDEKRPQLQRIY
                     GTAWETQEELETYLRRIEEAELRDHRRLGRDLDLFSFPDELGAGLSVWHPKGGVVRGE
                     LERWVRDLHVERGYELVATPHLARSVLWETSGHLEKFAESMYPAMSADDGSDYYVKPM
                     NCPFHVLIFRSRTRSYRELPLRLAELGTVYRYEKPGVLHGLLRVRGLTQDDSHIICTT
                     DQLTDEILGVFDLTLEIHRTFGFEDPVIHLSTQPGQAIGDEEMWERATRALREALDRS
                     GMPYELAEGEGAFYGPKVDFHFHDAIGRLWQLTTVQVDFALPERFEMEYMGEDNQRHR
                     PVMIHRAILGSVERFLGVLIEHFAGALPPWLSPVQARLIPIADRHAEHAEGLARRLRQ
                     GGLRVEVDASRETLGKKIRDAQLQKVPYALVVGDKEIEADTVSVRDRSGTEVRGVSVE
                     AFERRAAEEVGSRAVQTGPVGDLA"
     gene            2241..2729
                     /locus_tag="VF058_06565"
     CDS             2241..2729
                     /locus_tag="VF058_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX6208009.1"
                     /translation="MERLWTPWRMEYIKSATTGDDAGCIFCELPQEGDDERTLILARR
                     EGAFVILNRFPYNPGHLMVAPFRHVGELTDLEDEELTETVSLLRDAVRALRDEWEPSG
                     FNVGMNLGRVAGAGVPGHLHWHVVPRWHGDSNFMPVIGQTKVLPELLEETYGKLRPAF
                     EA"
     gene            2732..4048
                     /locus_tag="VF058_06570"
     CDS             2732..4048
                     /locus_tag="VF058_06570"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6208010.1"
                     /translation="MPEAARRIVRTYLAISGLFYLAASIIWGVNTLFLLDAGLTLFEA
                     FLANAAWTAGMVVFEIPTGLVADTRGRRVSFLLSASILALGTLGYVVISWVGGGIVPF
                     VLVSIVLGLAYTFYSGAVEAWLVDALRENGYTGELDPVFAKGGVVASTAMLVGTVGGG
                     ALGTFDLGFPYLARAALLLLAFGVAFVWMRDEGFTPRALRLRAVRREMGEVARQSVRH
                     GWGRPAVRLLMLVSFIQFGVGAWAFYAWQPYFLDLLGRGELIWVAGVVAALIALAEIC
                     GNALVRVVAKPGRRRSTILLVGAAAYAVSLAVVGMVSSFYLAVAVFVGGILVSAMATP
                     IKQAYLHEMIPSAQRATVISFDGLFGSLGSVGGQVGLGWLSQTRSIPAGFVVSGVSAL
                     FALPLLGLLRGRRERPDLIRSPAPDEAAAAGAAGAGGEAATDAAPR"
     gene            complement(4045..>4434)
                     /locus_tag="VF058_06575"
     CDS             complement(4045..>4434)
                     /locus_tag="VF058_06575"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX6208011.1"
                     /translation="GRILQTGIPHEVYERPSDPLVAGLLGDANLIPADVRGGLIETPL
                     GALPADGWRDGPVGLVVRPESIALEPDPSSEALVEDVEFYGHDQLFGVRLPDGSLLKV
                     RCFGTRDDLRVGTPVRARLLAAPRLYR"
BASE COUNT          585 a         1513 c         1673 g          663 t
ORIGIN      
        1 ctcgctcggg accgacgggt tcggacgctc cgacaccagg ccggcgctgc gccgcttcta
       61 cgaggtggac gcggagcacg tcgtcgtggc cacgctcgcg gcgctggccg agatgggcga
      121 ggtgaagccc gagcaggtgg cggaggcgat cgagcggtac gacatcgatc ccgagcgcgg
      181 ggcgccgttc caccccgacg cgggcacctc tctggcctga cctggcgccg ggagtgcgga
      241 aagggcaccc tcgggtcctc cgaccgctgc tgttcctgat cgtcaaggcg tatcagcggg
      301 ccgcgagatc gggggaggag gccgtccttc gcccgtgccc gtactgcacg acggacatcc
      361 cggtcgcggc gtcgcgctgc ccgagctgca cgagccaggc ggagccgctg gcgacctgac
      421 gacggctatc atggcgtccg cgcgcccggc gcggtgaagg agtgagggat ggcggacgct
      481 gatcggctcg aggtcctgcg gcactcgacc gcccacgtga tggcccaggc ggtgtgcgac
      541 ctcttcgagg gggcgaagta cgcgatcggt cccccgatcg aggacggctt ctactacgac
      601 ttcgacctgc ccgagacgct caccccggag gacctggagc ggatcgaggc ccgcatgcgc
      661 gagatcatcg gggaggacca gcccttcgtc cgcgaggagc tctcgcggga cgaggccctg
      721 gagcggttcg ccggccagcc ctacaagatc gagatcatcg ccggcgtgga ggaggaggag
      781 ggcgccggcg ccgagggcgt ctccgtctac cgcaacgacg gctgggccga cctctgccgg
      841 ggcccccacg tcgggtccac ggccgagctg ggcgccttca agctgatgag cgtggccggt
      901 gcctactggc ggggcgacga gaagcgcccc cagctccagc ggatctacgg cacggcttgg
      961 gagacccagg aggagctcga gacctacctt cggaggatcg aggaggccga gctgcgcgac
     1021 caccgccggc tgggtcgcga cctcgacctc ttctcgttcc ccgacgagct cggggcgggc
     1081 ctctccgtct ggcacccgaa gggcggggtg gtgcggggcg agctggagcg gtgggtgcgg
     1141 gacctccacg tggagcgcgg ctacgagctc gtagccaccc cccacctagc ccggtcggtg
     1201 ctgtgggaga cgagcggcca cctggagaag ttcgccgaga gcatgtaccc ggcgatgtcg
     1261 gccgacgacg ggtcggacta ctacgtcaag ccgatgaact gcccgttcca cgtcctgatc
     1321 ttccggtcgc gcacgcgctc gtaccgggag ctgccgctgc gcctggccga gctgggcacg
     1381 gtgtaccggt acgagaagcc gggggtgctg cacggcctgc tccgcgtgcg agggctcacc
     1441 caggacgact cgcacatcat ctgcacgacg gaccagctga ccgacgagat cctcggggtc
     1501 ttcgatctca cgctcgagat ccaccggacg ttcgggttcg aggacccggt gatccacctc
     1561 tcgacccagc cgggacaagc catcggcgac gaggagatgt gggagcgggc cacgcgggcc
     1621 ctgcgcgagg cgctggaccg gagcggcatg ccctacgagc tcgctgaggg cgagggtgcc
     1681 ttctacgggc cgaaggtgga cttccacttc cacgacgcga tcgggcgcct gtggcagctc
     1741 acgaccgtgc aggtcgactt cgccctgccc gagcggttcg agatggagta catgggcgag
     1801 gacaaccagc ggcaccgccc ggtgatgatc caccgggcga tcctggggtc ggtggagcgg
     1861 ttcctggggg tgctgatcga gcacttcgcg ggtgcgctcc cgccgtggct ctcgccggtg
     1921 caggcccggc tcatcccgat cgccgatcgg cacgctgagc acgccgaggg tctcgcgcga
     1981 cgcctgcgcc agggcgggct gcgcgtcgag gtcgacgcgt ctcgcgagac cctcggcaag
     2041 aagatccggg acgcccagct ccagaaggtc ccctacgccc tcgtggtggg ggacaaggag
     2101 atcgaggccg acaccgtctc ggtccgcgac cggtcgggga ccgaggttcg cggggtctcg
     2161 gtggaggcgt tcgagcggcg ggccgcggag gaggtcgggt cccgggccgt gcagaccggg
     2221 cccgtcgggg acctcgcgtg atggagcgtc tctggacccc ctggcgcatg gagtacatca
     2281 agtcggcgac cacgggcgac gacgccggct gcatcttctg cgagctgccg caggagggcg
     2341 acgacgagcg gacgctgatc ctggccaggc gcgagggggc gttcgtgatc ctcaaccggt
     2401 tcccctacaa ccccggccat ctcatggtgg cgcccttccg gcacgtcggc gagctcaccg
     2461 acctcgagga cgaggagctg accgagaccg tgtccctgct ccgggacgcc gtccgagcgc
     2521 tccgcgatga gtgggagccc agcggcttca acgtgggcat gaacctgggg cgggtggcgg
     2581 gcgccggtgt gccggggcac ctccactggc acgtcgtgcc tcgctggcac ggcgactcca
     2641 acttcatgcc ggtgatcggc cagaccaagg tgttgcccga gctcctcgag gagacgtacg
     2701 ggaagctgcg acccgcgttc gaggcctgac ggtgccggag gccgctcgaa ggatcgtccg
     2761 gacctacctc gcgatcagcg gactgttcta cctagccgcc tcgatcatct ggggggtcaa
     2821 caccctcttc ctgctcgacg ccggcctgac gctgttcgag gcgttcctgg ccaacgcggc
     2881 ctggaccgcg gggatggtcg tgttcgagat cccgacgggc ctcgtggccg acacgcgggg
     2941 gcgcagggtc tcgttcctcc tctcggcctc gatcctggcc ctgggcaccc tcggctacgt
     3001 cgtgatctcg tgggtcggcg gggggatcgt gccgttcgtg ctcgtctcga tcgtgctggg
     3061 cctggcctac accttctact cgggcgccgt ggaggcgtgg ctcgtggacg cgctccgcga
     3121 gaacggatac acgggcgagc tcgacccggt cttcgccaag ggcggggtcg tggccagcac
     3181 cgcgatgctc gtggggacgg tgggcggcgg cgcgctcggg accttcgacc tcggcttccc
     3241 gtacctggcc cgggccgccc tcctcctgct ggcgttcggc gtggcgttcg tctggatgcg
     3301 ggacgagggg ttcacgccga gggccctgcg cctgcgggcg gtgcgtcggg agatgggcga
     3361 ggtcgcccgg cagagcgtgc ggcacggctg ggggcgcccg gccgtccggc tcctgatgct
     3421 cgtctccttc atccagttcg gcgtgggggc ctgggccttc tacgcctggc agccctactt
     3481 cctcgacctc ttgggacggg gggagctgat ctgggtggcg ggggtcgtgg cggcgctgat
     3541 cgcgctggcc gagatctgcg ggaacgcgct cgtgcgcgtc gtggccaagc ccggccgccg
     3601 ccgctcgacg atcctcctcg tcggggcggc cgcctacgcc gtctcgctcg ccgtcgtggg
     3661 gatggtgtcg agcttctacc tcgcggtcgc ggtcttcgtg ggggggatcc tcgtctcggc
     3721 gatggccacg ccgatcaagc aggcctacct ccacgagatg atcccgtcgg cccagcgggc
     3781 caccgtgatc tcgttcgacg ggctcttcgg gagcctgggg agcgtcggcg gccaggtggg
     3841 cctgggctgg ctgtcccaga cgcgctcgat cccggccggg ttcgtcgtgt cgggcgtctc
     3901 cgcgctgttc gccctcccgc tgctgggctt gctgaggggg aggcgggagc gccccgacct
     3961 gatccgcagc ccggctcccg acgaggcggc ggcggcgggg gccgcgggcg cgggaggcga
     4021 ggcggcgacg gacgccgcgc cgcgctagcg gtaaaggcgc ggggcggcga ggagtcgggc
     4081 ccgcaccggc gtgcccaccc ggaggtcgtc gcgggtcccg aagcagcgga ccttgagcag
     4141 gctgccgtcg gggagccgca cgccgaagag ctggtcgtgg ccgtagaact ccacgtcctc
     4201 cacgagggcc tcgctcgagg ggtcgggctc cagggcgatg ctctcgggcc gcaccacgag
     4261 gcccaccggc ccgtcccgcc agccgtcggc cggcagggcc ccgagcggcg tctcgatcag
     4321 gccgccccgg acgtccgcgg ggatcaggtt cgcgtcgccc agcagcccgg ccacgagcgg
     4381 gtccgagggc cgctcgtaga cctcgtgggg gatgccggtc tggaggatcc tccc
//
LOCUS       DASUTW010000190         8213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_578568, whole genome shotgun sequence.
ACCESSION   DASUTW010000190 DASUTW010000000
VERSION     DASUTW010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8213
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578568"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /locus_tag="VF058_06580"
     CDS             <1..1401
                     /locus_tag="VF058_06580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX6208012.1"
                     /translation="LVVRYRKGSARERRQLRWVLLAAAVTVGAAIWPFLLGELGVLPA
                     VTHGIWATILWLPILLLPVSIVVAVLEPAWLDVDLAIRKTFVYGTLSFLILALYVGVA
                     TVSGVAAGAALPLEVAILVAVGVAALFQPARRWLQSVADRWVFGEPPAGYEAVTDFTT
                     AVREATDPGDLVPRIADTTRRALGLRWVGATVEGERPVVVGERTGRPELVAPIEHQGD
                     RFGRIECGPKSKGALTDADVELVTTLASQAALALFNARLAGRIVYTQEAERRRIERNI
                     HDGAQQELVALVARLGMARADLKRDRLSEETLVELQREASRILSDLRELAQGIHPSVL
                     TDGGLLDAIEDRCGRLPIDVSLEATPGLRARRFPDEVEGAAYFFVTEGVANVLKHARA
                     SRARIAVRHEDGVLALDVSDDGVGFDPWRVRRTGLSGLSDRIAALGGTFAVTSRPGEG
                     ASLRAVLPIESSGASV"
     gene            1398..2093
                     /locus_tag="VF058_06585"
     CDS             1398..2093
                     /locus_tag="VF058_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6208013.1"
                     /translation="MSDRLRVVIAEDHYLVREGTRGLLESSGEVEVAAAVEDAPSLLD
                     AVERLHPDVVLVDIRMPPTHQTEGIDAARRIRAAHPEVGVVVLSQHVSSHYAFELFRD
                     GTSGLAYLLKDRVGDLDELLAALRSVADGGSVIDPLVVERLLAQGERLSGTGLSELSP
                     REMEVLAKMAEGKSNTAIAGALFVSQSAVEKHINSIFAKLGLSVDEGETHRRVAAVLA
                     FLRSRDQSRETQG"
     gene            2090..3031
                     /locus_tag="VF058_06590"
     CDS             2090..3031
                     /locus_tag="VF058_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004559817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6208014.1"
                     /translation="MSAVLRVAGLRKSYGAVEALQGVDLEVARGEVVALLGPNGAGKT
                     TVVSIVAGLRRPDAGTVEVDGLDALRRSADVRRRIGLAPQELGIYPVVSVRNNLALFG
                     RLAGLSGAELRSRIEDVAEALRITDLLDRQGGTLSGGQKRRVHTAIALLHRPPLLLLD
                     EATTGADVETRGHILDLVRRLAAGGSAVLYSTHYLQEVEDLGATVVLLDGGRVIARGG
                     AAELTAAHATTELELIFEGEVPALGLDGSAIVEGSRLRVPVQDPAATLATLIPRVPSG
                     RLRSVEIVRPDLEAVYLALTGRRYEEADEEEERVAAG"
     gene            3042..3788
                     /locus_tag="VF058_06595"
     CDS             3042..3788
                     /locus_tag="VF058_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6208015.1"
                     /translation="MRHEFRVLMTDPSTFVFLIAMPLVMAALTRQLFQGALAAEGFRG
                     ANGSEFAIPGMAVSFAAFGVGYSGFTFFRDHGWGTWDRLRATPATSVDLIAGKVVPTL
                     LVTGLQLGLLFAVGGPLLGLRVAGSGAALGVTIVALALALAAFGLAVTAVARTMQQLN
                     AIGSVGGFAMAIIGGAFVPVSSMPDWAQAVAPVMPTYWAMKAFSSVILEGGGMGAVLV
                     PVATMLAFAILFGLLAAAKFRLEDTKAYYG"
     gene            complement(3908..5470)
                     /locus_tag="VF058_06600"
     CDS             complement(3908..5470)
                     /locus_tag="VF058_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX6208016.1"
                     /translation="MSQQPAVDPESVRAAEIIAAACLATDLGMGFPFEHGLHGTLMAM
                     RLADLLAVDAETASQTYYASLLMYSGCMTDAHIATRIFQGSRTEHLTPKQFGSARESL
                     GGVIRSLPDPEAAGLGRAAQIAGRLVVAARFRKPHFAALCEVAEMMAERLGLPPAVHG
                     LFAYLTERWDGKGILRRGREGEIPLPIRIVQVARDAAYQRVLGGEQRALAMTRERAGH
                     AFDPDVATRFVEHAPEVFGAVDEAASSWEATLEAEPRPHLTLEDGAIDRALAAMGDFA
                     DLGSPHLAGHSRGVADLAASAARLCGFDGQEVVQIRRAAMVHDLGRTAIDPRIWQKAD
                     ALSVDEWEQVRLHPYHTERVLVRSPALAPLADVACSHHERLDGGGYHRRAAAASLTGG
                     ARLLAAADAFHAMCEPRPHRPPLQPDRAAEVLGQEARAARLDSRMVAAVIEAAGQPPP
                     PVERPAGLTEREVEVLGLLARGLQTKQVARRLGISNKTADRHIQNAYRKIGISTRAAA
                     TLFAMEHGLVPR"
     gene            5574..6197
                     /locus_tag="VF058_06605"
     CDS             5574..6197
                     /locus_tag="VF058_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020026641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEX6208017.1"
                     /translation="MDDYPVRFEVDYPDRALDRLTTFFRLIWAIPILILFGTIAGGTW
                     QWSYEESRYVAAGAGGLLFFGPLLMIVFRQKYPRWWFDWNLAWLRFSNRVSAYLALLD
                     DRYPSSDEEQAVHLEIPYPDAQEDLNQFLPLVKWFLAIPHYVVLAFLYIGGLFAVIFA
                     WFAILFTGRYPKGLFDFVVGVFRWHNRVVGYAFVLVTDRYPPFSLAG"
     gene            6253..6558
                     /locus_tag="VF058_06610"
     CDS             6253..6558
                     /locus_tag="VF058_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208018.1"
                     /translation="MIRIPVERRSPTHVVLLLALVILAIVLLWHLFGMEHSEGMGILG
                     GCLFLLTAVLVLVVPTIRWPLRVPVEARREGPPTAALHPVSRPPPIEASLASVVLIN"
     gene            6610..7158
                     /locus_tag="VF058_06615"
     CDS             6610..7158
                     /locus_tag="VF058_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208019.1"
                     /translation="MSYPQRRTRWWAGIAVVAVTMLLATACADTETGDGGEGGTVDVT
                     LQEWSVVAGQDSVAAGPVTFNVTNEGPDHPHELVVIRTDLDPAELPTTDEGAVDEEGE
                     GIEVIGEIEEFPPGETHDATFDLEPGSYVLVCNVVAVAGEHGEGHEEEEEMMEEEGEH
                     EGMEEMEIHYMLGMRAPFTVTG"
     gene            7287..7688
                     /locus_tag="VF058_06620"
     CDS             7287..7688
                     /locus_tag="VF058_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208020.1"
                     /translation="MRTGRVLLGLVAFWGAIGVVLAVVALSQPPSEESPPAATPALRT
                     VVVSDAAPSQPSSPDGPVEVALLRGDAPSEPGVATVLSDENCEPDANGYSHCLNELEM
                     EGGERIAVRHTHRMGEVPCLAPGERVNVRLS"
     gene            complement(7675..>8213)
                     /locus_tag="VF058_06625"
     CDS             complement(7675..>8213)
                     /locus_tag="VF058_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEX6208021.1"
                     /translation="PFRSLAEPVADLLRPMPYSEMFPPEEEEYRPTAVAQTMLIQEID
                     AGAAEMIVERLQASDAAMRAAQLRVLGGAMARVPVDSTAFAHRDARIMVNLAAFYEGP
                     EDRPRRQAWVDEFAAALRGEGHGAYVAFLGDEGEERVHDAYPAGTWERLSEVKARYDP
                     TNLFRLNQNVLPAAATTT"
BASE COUNT         1065 a         2827 c         3063 g         1258 t
ORIGIN      
        1 ctcgtcgtcc gctatcggaa gggctccgcg cgagagcgga ggcagctcag gtgggtgctg
       61 ctggccgcgg cggtgaccgt cggcgcggcg atctggccgt tcctcctggg ggagctcggc
      121 gtcctgcccg cggtgaccca cgggatctgg gcgaccatcc tgtggctgcc catcctcctg
      181 ctcccggtct cgatcgtggt ggccgtcctg gaaccggcgt ggctggacgt ggacctcgcc
      241 atccgcaaga cgttcgtgta cgggaccctc tccttcctca tcctggcgct ctacgtgggg
      301 gtggccaccg tttccggcgt cgccgccgga gcggcgcttc ccctggaggt ggcgatcctg
      361 gttgccgtcg gggtcgcggc gctcttccag ccggcccgtc gctggctgca gtcggtcgcc
      421 gaccggtggg tcttcggtga gcccccggcc ggctacgagg ccgtcaccga cttcaccacg
      481 gccgtcaggg aggccaccga tccgggtgac ctcgtccccc ggatcgccga cacgacgcgc
      541 cgcgccctcg ggctgcggtg ggtcggcgcg acggtggagg gcgaacggcc cgtcgtcgtg
      601 ggggagcgca cggggcgccc cgagctcgtg gcgccgatcg agcatcaggg tgaccggttc
      661 ggccgcatcg agtgcggccc caagtcgaag ggcgcgctca ccgacgccga cgtcgagctc
      721 gtgacgacgc tggcgagcca ggccgcgctc gccctgttca acgcccgcct cgcgggacgg
      781 atcgtctaca cgcaggaggc cgagcggcgc cgcatcgagc gcaacatcca cgacggcgcg
      841 cagcaggagc tcgtggcgct ggtggccagg ctggggatgg cccgcgccga ccttaagagg
      901 gatcgactct ccgaggagac gctcgtcgag ctgcagcggg aggcgagccg catcttgtcc
      961 gacctccggg agctggccca ggggatccat ccgtcggtgc tcaccgacgg agggctgctc
     1021 gatgcgatcg aggatcggtg cggccgcctc ccgatcgacg tgtcgctcga ggccacgccc
     1081 ggcctccggg cgaggcgctt ccccgacgag gtcgaggggg ccgcctactt cttcgtcacg
     1141 gagggggtgg ccaacgtcct gaagcacgcc cgggcctccc gggcccgcat cgccgtccgc
     1201 cacgaggacg gggtcctcgc cctcgacgtc agcgacgacg gggtggggtt cgatccgtgg
     1261 cgggttcgga ggacgggtct ctccgggctc tcggaccgga tcgccgccct cggggggacg
     1321 ttcgccgtga cgagccgtcc gggggaggga gcctcgctcc gggccgtcct cccgatcgag
     1381 tcgtcgggag cgtcggtgtg agcgatcggc tccgcgtcgt gatcgccgag gaccactacc
     1441 tggttcgcga ggggacgcgg gggctgctcg agtcctcggg cgaggtggag gtggcggccg
     1501 cggtggagga cgcgccgtcg ctcctcgacg ccgtggagcg gctccacccc gacgtggtgc
     1561 tggtcgatat ccggatgccc cccacccacc agaccgaggg catcgacgcc gcgcgccgga
     1621 tccgcgccgc ccacccggag gtcggcgtgg tggtgctctc ccagcacgtg agctcgcact
     1681 acgccttcga gctgttccgg gacgggacca gcggcctggc ctacctcctg aaggaccgag
     1741 tgggggacct cgacgagctg ctggcggcgc tgcggtcggt cgcggatgga ggatcggtga
     1801 tcgacccgct ggtcgtggag cggctgctgg cgcagggtga gcggctctcg gggaccgggc
     1861 tctcggagct cagcccgcgg gagatggagg tcctggccaa gatggccgag gggaagtcga
     1921 acacggcgat cgcgggcgcg ctcttcgtgt cgcagtcggc ggtggagaag cacatcaact
     1981 cgatcttcgc caagctgggg ctgagcgtgg acgaggggga gacgcatcgg cgggtggcgg
     2041 cggtgctcgc cttcctgcgg agccgcgatc agagccggga gacgcaggga tgagcgccgt
     2101 gctgcgcgtc gccggcctcc gcaagtcgta cggcgccgtc gaggccctgc agggcgtgga
     2161 cctggaggtc gcccgcggcg aggtcgtggc gctcctgggc ccgaacgggg cggggaagac
     2221 cacggtggtc tcgatcgtgg ctgggctccg gcggcccgat gcggggacgg tggaggtcga
     2281 cgggctcgat gccctgcgcc gctcggccga cgtgcgtcgg cgcatcgggc tggccccgca
     2341 ggagctgggg atctaccccg tggtgtcggt caggaacaac ctggcgctgt tcgggcggct
     2401 ggccgggttg agcggggccg agctcaggtc gcggatcgag gacgtcgccg aggcgctgag
     2461 gatcaccgac ctcctcgacc ggcagggggg caccctctcg ggcgggcaga agcgcagggt
     2521 ccacaccgcg atcgccctgc tccatcgccc gcccctgctg ctgctggatg aggccacgac
     2581 cggcgcggac gtcgagacgc gcggccacat cctcgatctc gtgcggaggc tcgcggccgg
     2641 cgggtcggcg gtcctgtact cgacccacta cctccaggaa gtggaggacc tcggagccac
     2701 cgtggtcctc ctcgacgggg gccgggtgat cgcccgaggc ggggctgccg agctcaccgc
     2761 ggcccacgca acgaccgagc tggagctgat cttcgagggt gaggtgcccg ccctgggcct
     2821 cgacggctcg gcgatcgtcg agggctcacg cctcagggtg cccgtgcagg atcccgccgc
     2881 cacgctcgcc accctgatcc cccgcgtccc gtcggggagg ctccgctcgg tggagatcgt
     2941 ccgccccgac ctggaggcgg tgtacctggc gctcacgggg cggcgctacg aggaggccga
     3001 cgaggaggag gagcgtgtcg ccgcgggcta gcctcgccat catgcgtcac gagttccggg
     3061 tgctgatgac ggacccctcg acgttcgtct tcctcatcgc catgcccctc gtgatggccg
     3121 cgctgacgag acagctcttc cagggggcgc tggccgccga ggggttccgg ggagcgaatg
     3181 gatcggagtt cgccatcccc gggatggcgg tgagcttcgc cgcgttcggc gtcgggtatt
     3241 caggcttcac gttcttccgg gatcatgggt ggggcacctg ggaccggctg cgggcgacgc
     3301 cggccacgtc ggtggacctg atcgcgggca aggtcgtccc caccctgctc gtcaccggcc
     3361 tgcaactcgg gctgctgttc gcggtagggg ggccactgct gggcctgcgc gtcgcggggt
     3421 cgggggcggc cctcggggtg acgatcgtgg ccctggccct cgccctggcc gccttcgggc
     3481 tggcggtgac ggccgtggcc cgcacgatgc agcagctgaa cgctatcggg tcggtcgggg
     3541 gcttcgcgat ggccatcatc ggcggggcgt tcgtcccggt ctcgtcgatg ccggactggg
     3601 cgcaggccgt cgcgccggtg atgccgacgt actgggcgat gaaggcgttc agctcggtga
     3661 tcctcgaggg cggcggcatg ggtgccgtcc tcgtgccggt ggcgaccatg ctggcgttcg
     3721 cgatcctctt cggactgctc gccgccgcca agttccgcct cgaggacacc aaggcctact
     3781 acgggtgacc gagccgggga ctacccgccg actattgcgc gcttcccgtg gcgggctgcg
     3841 cacgaggccc tcggatcgac gctaggaggc ggtcgcgccg agtgtgaatc gggagttctc
     3901 tccccagtca gcgggggacc agcccgtgct ccatcgcgaa cagcgtggcc gccgccctcg
     3961 tggagatccc gatcttccgg tacgcgttct ggatgtggcg gtcggcggtc ttgttcgaga
     4021 tccccaggcg ccgcgccacc tgcttcgtct ggaggccgcg ggccaggagc ccaaggacct
     4081 cgacctcgcg ctccgtgagc ccggcggggc gctccacggg gggagggggt tgtcccgccg
     4141 cctcgatcac cgcagccacc atccgcgagt cgagacgggc ggcgcgcgcc tcctggccca
     4201 ggacctccgc ggcccgatcc ggctggaggg gcggacggtg gggcctcggc tcgcacatgg
     4261 cgtggaaggc gtcggcggcg gccaggaggc gcgcgccgcc cgtgagggag gccgctgcgg
     4321 cccgccggtg atacccgccg ccgtccagcc gctcgtgatg ggagcaggcc acgtccgcga
     4381 ggggggcgag cgccggggag cggaccagca cccgctcggt gtggtaggga tgcagccgca
     4441 cctgctccca ctcgtccacg gagagcgcgt cggccttctg ccagatccga gggtcgatcg
     4501 cggttcgccc caggtcgtgc accattgcgg ctcggcggat ctggacgacc tcctgcccgt
     4561 cgaagccgca cagtctggcg gcggaggcgg ccaggtcggc cacccccctc gagtggcccg
     4621 cgagatgggg cgacccgagg tccgcgaagt cccccatggc ggcgagggcc cggtcgatcg
     4681 ccccgtcctc gagcgtcagg tgcggccgcg gctcggcctc gagggtggcc tcccacgacg
     4741 acgcggcctc gtccaccgcg ccgaagacct ccggcgcgtg ctccacgaac cgcgtcgcga
     4801 cgtccgggtc gaaggcgtgc cccgcgcgct cacgggtcat cgccagcgca cgctgctcgc
     4861 cgcccaggac ccgctggtag gcggcgtccc gcgccacctg cacgatcctt atcgggaggg
     4921 ggatctcgcc ttcccgcccg cgccgcagga tccccttccc gtcccagcgc tcggtgaggt
     4981 aggcgaagag accgtgtacc gccggcggca ggccgagccg ctcggccatc atctcggcca
     5041 cctcgcacag ggcggcgaag tgcggcttcc ggaaccgcgc cgcgacgacg aggcggccgg
     5101 cgatctgggc ggcgcgcccg agcccggcgg cctccgggtc ggggagcgag cggatcaccc
     5161 ctcccagcga ctccctcgcc gagccgaact gcttgggcgt gagatgctcc gtcctgcttc
     5221 cctggaagat gcgcgtggcg atgtgcgcgt cggtcatgca gcccgaatac atgaggaggc
     5281 tcgcgtagta ggtctgcgac gcggtctcgg catcgaccgc caacaggtcg gccagccgca
     5341 tggccatcag cgtgccgtgg aggccgtgct cgaacgggaa ccccatcccc aggtcggtgg
     5401 cgaggcatgc ggcggcgatg atctccgcgg ctcgaaccga ctcggggtcg actgcaggct
     5461 gctgcgacac ctcgaccagg gtacgcgagg ctcggttccg ctcgcgaccc gggtggcggg
     5521 ggcggccctc ctgatgcgag gctaacgagg tcccgagatc gagggggaag ctgatggacg
     5581 actatcccgt gcggttcgag gtggactacc cagaccgagc cctggaccgc ctgaccacct
     5641 tcttccggct catctgggcg atccccatcc tgatcctgtt cgggacgatc gcggggggaa
     5701 cctggcagtg gagctacgag gagagtcgtt acgtggcggc gggcgccggg ggcctcctct
     5761 tcttcggccc cctgctgatg atcgtgttcc ggcagaagta tccgcgctgg tggttcgact
     5821 ggaacctggc gtggcttcgc ttctcgaacc gggtctccgc gtacctggcg ctgctcgacg
     5881 atcggtatcc ctcgtcggac gaggagcagg ccgtccacct cgagatcccg tacccggacg
     5941 cgcaggagga cctgaaccag ttcctcccgc tcgtgaagtg gttcctggcg atcccgcact
     6001 acgtcgtgct tgcgttcctg tacatcgggg ggctcttcgc cgtgatcttc gcctggttcg
     6061 ccatcctgtt caccgggcgc tacccgaagg ggctgttcga cttcgtggtg ggcgtgttcc
     6121 gctggcacaa ccgggtggtc ggctacgcgt tcgtgctcgt gaccgatcgg tacccgccgt
     6181 tctcgctcgc gggctgatcc ccgggccgtc cgttggggcc ctgctgaaag aggctgtggt
     6241 agcgtcgggg aggtgattcg gatccctgtc gagcgacgct cgccgaccca cgtcgtgctc
     6301 ctgctcgcgc tcgtcatcct cgcgatcgtc ctgctgtggc acctcttcgg gatggagcac
     6361 tccgagggca tgggcattct cggggggtgc ctgttcctcc tgacggcggt gctggtgctg
     6421 gtcgtgccca ccatccggtg gcccctgcgg gtgcccgttg aggctcggcg agagggtccg
     6481 cccacggccg cgctgcatcc ggtgtcgcgc ccgcctccga tcgaagcctc gctcgcgagc
     6541 gtcgtgctga tcaactgagt ccactccttc cgctgtcctg ttcccaagca gccgaaagga
     6601 gagactcaca tgtcgtatcc acagcggagg acccgatggt gggccggcat cgccgtcgtc
     6661 gcggtgacca tgctcctcgc gacggcatgc gccgacacgg agaccggtga cggcggcgag
     6721 gggggaaccg tcgacgtgac cctccaggag tggtccgtcg ttgctggtca ggactcggtg
     6781 gcggccggtc ccgtgacgtt caacgtcacc aacgaggggc cggaccatcc tcacgagctc
     6841 gtcgtgatca ggacggacct cgaccccgcg gagctgccca cgacggacga gggcgccgtg
     6901 gacgaggagg gggaggggat cgaggtcatc ggcgagatcg aagagttccc cccgggggag
     6961 acgcacgatg ccacgttcga tctcgagccc gggagctacg tcctggtctg caacgtcgtc
     7021 gcggttgccg gcgagcacgg cgagggccac gaagaggagg aggagatgat ggaagaggag
     7081 ggtgagcacg agggcatgga ggagatggag atccactaca tgctgggcat gcgcgctccc
     7141 ttcaccgtca ccggataggc ggccgggccg acggaggtcc cggacgagag gggtccttcg
     7201 gccctcctcc cggggcctcc ggacgccgag tatccgacgc gcaggcaacg tggatcacgg
     7261 gcgacacgat gaggagggag ccgaggatgc ggacgggacg ggttctgttg gggctggtgg
     7321 cgttctgggg cgcgatcggc gtcgtcctgg ccgtcgtcgc cctgagccag ccgccgtccg
     7381 aggagagccc gccggccgcg accccggccc tgcgcacggt cgtggtttcc gacgccgcgc
     7441 cctcgcagcc ctcgagcccc gacgggccgg tggaggtcgc gctgctgcgc ggagacgcgc
     7501 catccgagcc gggcgtggcc acggttctca gcgacgagaa ctgcgagccg gacgcgaacg
     7561 gctactcgca ctgcctcaat gagctcgaga tggaaggcgg cgaacggatc gccgtccgcc
     7621 acacgcaccg gatgggcgag gtgccctgcc tcgccccggg cgagcgcgtg aacgtcaggt
     7681 tgtcgtagcc gccgccggca ggacgttctg gttcaggcgg aagaggttcg tcgggtcgta
     7741 gcgggccttg acctcgctca gccgctccca cgtcccggcg gggtaggcgt cgtgcacgcg
     7801 ctcctcgccc tcgtctccca ggaaggccac gtacgcgccg tgcccctcgc cgcggagcgc
     7861 cgccgcgaac tcgtcgaccc acgcctggcg ccgggggcgg tcctccgggc cctcgtagaa
     7921 ggccgcgagg ttgaccatga tgcgcgcgtc acgatgggcg aacgccgtgg agtcgaccgg
     7981 cacccgcgcc atcgcccctc cgagcacgcg cagctgcgcg gcccgcatcg cggcgtccga
     8041 cgcctggagg cgctccacga tcatctcggc ggcgcctgcg tcgatctcct ggatcagcat
     8101 cgtctgagcg accgccgtgg ggcggtactc ctcctcctcg ggagggaaca tctccgagta
     8161 cggcatgggc ctcagcaggt ccgcgacggg ctcggcgagc gaccggaacg ggg
//
LOCUS       DASUTW010000191         7442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1095373, whole genome shotgun sequence.
ACCESSION   DASUTW010000191 DASUTW010000000
VERSION     DASUTW010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7442
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095373"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1024)
                     /locus_tag="VF058_06630"
     CDS             complement(14..1024)
                     /locus_tag="VF058_06630"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018893298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX6208022.1"
                     /translation="MTLPISPPLAPMLSKSRDELPRGEGWVYEPKWDGFRAIVFRDGE
                     DVHIGSRTKRPLHRYFPELLGPLRKALPERCVVDGEIVLARDGRLDFEALQLRLHPAE
                     SRVRMLSEAIPASFVGFDLPALGDEDLCDRPLSERWDLLEGILEERSIRDLLRDGPIV
                     ARTPRTEDPEEAASWMEELGAFGLDGVIAKTGDLTYRPGERAMVKVKKRRTADCVVGG
                     YRIHKDGEGVGSLLLALYAEDGTLHHVGFTASFRAKQRRELLDVVRPHHGESAFGEHA
                     GPGGPSRWRQEETPWIPLRPELVCEVAFNHTTGRRIRHGATFVRWREDKDPRDCTMDQ
                     IA"
     gene            1075..1269
                     /locus_tag="VF058_06635"
     CDS             1075..1269
                     /locus_tag="VF058_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208023.1"
                     /translation="MPREIDMQLFEQAVNVTATALRGAMGGEKSQPPSYAAELFREVW
                     EALKEASKDLPDRSRAGFGS"
     gene            complement(1270..1644)
                     /locus_tag="VF058_06640"
     CDS             complement(1270..1644)
                     /locus_tag="VF058_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6208024.1"
                     /translation="MQTVGDVMTSGVLRVTPATTVAEAATMMGERRVGSALVMEDDRL
                     LGIFTERDIVRALGQDFGAAERPVSEWMTSEPTTVPPETPASEALKTMLDGGFRHLPV
                     SEGDRVLGIVSIRDLSAAGLAE"
     gene            1732..2595
                     /locus_tag="VF058_06645"
     CDS             1732..2595
                     /locus_tag="VF058_06645"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEX6208025.1"
                     /translation="MKRLWIGIVAAGLVLAACGGGGTEEADGAGPVPGVAGERAAEGL
                     QVEDAAGDEALGDGAAAEAAAAELPVLGPSVIKSAQIGLEVERGGFKDALQRATSVAS
                     AEGGFVLSSSVEGKEARRGSIVLRVPSDRFEAALASLRDLGTVRRERIEGEEVGQEFV
                     DLEARLRNLRAQEAVLLRLMDEAGSIAETIRVQRQLTGIQLQIERIRGRLRYLEDRTS
                     FGTISVSLAEVGVAAPREPSIIARAWERAAEGFLSVIGGLITALGLLTPIAVLALLAW
                     LLLRRRLPAKA"
     gene            complement(2616..3635)
                     /locus_tag="VF058_06650"
     CDS             complement(2616..3635)
                     /locus_tag="VF058_06650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6208026.1"
                     /translation="MRFGIGLFTAQVPPGSGRGFVDEYRDVVELARLAERVGFDSVWV
                     SEHHGAGDGYMPSLLPVLGALAAVTDRVLLGTGVMLAPFHDPLRLAEDAATVDLLSDG
                     RLILGLGLGWREEEFRMWGIPIGERLRRTTETVEILRKAWTGERFSHEGRVFSYDRVR
                     ITPPPAREGGPPIYLGGFAEAPVRRAGRLGDGYIVSRSVLDVVHRNLAWAEEGARKAG
                     KDPSDLGFAILRNAFVAREGAWEAILPGVSHQLGVYPAWGAGADTPDRPFEVHPPDEE
                     TMRSVTTAGTPKEVVGDLRPFVDAFGARRDSHLILRLHYPGMDRETAAAAIELFAADV
                     LPALR"
     gene            complement(3637..4128)
                     /locus_tag="VF058_06655"
     CDS             complement(3637..4128)
                     /locus_tag="VF058_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX6208027.1"
                     /translation="MTLDPERLLDETGWRILRELQENARITFSELGRRVALTAPAVAD
                     RVRRLEEAGVIRGYRVEVDREALGLPVLAFIRITATGAECRALAAEISGFPEVLECHR
                     VTGEEAFIAKVAVRSVAHLQDLIDRLMPYGETITSIVLSSPVSYRTIDRESANGEVAA
                     RRR"
     gene            4223..5242
                     /locus_tag="VF058_06660"
     CDS             4223..5242
                     /locus_tag="VF058_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019867778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX6208028.1"
                     /translation="MARPGSMLSSPQDPTHGGGHAPTWAIWSAMWAVYIVWGSTYLAI
                     AVVVETFPPFLSAAIRFLVAGALLYAWAIRRGDRVGDRPTLRHWRTAAIVGGALLLGG
                     NGMVVWAEQTVPSGIAALLVGTVPLWMVVVSGLVLRERTSWREVLGIVIGFGGVALLV
                     GPIGGGGTFDPWGVAAVVFASISWASGSLYARRAPLPERPLVGTAMEMLAGGVVLTVV
                     AAITGEFGRVDWGAISLASWIGVAYLVVFGSLVGFAAYAWLLRVAKTSLVATYAYVNP
                     VIAVLLGWAFLREPVTARTLVAGVVIVGAVALIVSARAEQGEEEPSGELGGASVEPPD
                     EQLTA"
     gene            complement(5232..5495)
                     /locus_tag="VF058_06665"
     CDS             complement(5232..5495)
                     /locus_tag="VF058_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208029.1"
                     /translation="MAEITVEPQGDAFFVRVDDGSTQTTHTVTVSDEDFERLGSGYGS
                     REDFVTACFEFLLEHEPKESILSTFDVSVIPRYFPEFEDEIRR"
     gene            5535..6908
                     /locus_tag="VF058_06670"
     CDS             5535..6908
                     /locus_tag="VF058_06670"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX6208030.1"
                     /translation="MQATELARGIAAREISPVEVLERALLRAEHWDLVSNVFSMLHAE
                     QAQEVARERQEEAARGELWGPLHGVPVAVKDLFDVEGWETTGCCRAYRGAVAERDAAV
                     VARLRAAGAVIVGKTNMHELAAGGTNAVSACGACRNPWDPSRISGGSSGGSAAAVGAR
                     VVPLALGTDTGGSVRIPASMCGTVGLKTTFGSLDMAGVMPLTRTLDTVGPLTMTVEDA
                     ALGLAVIAGRGQRFVDETAASPEGLRVAVPGAFFAERARRDVLAAVHEARDVLIGMGG
                     RPATGEVGHVGYANDAWINIAWPEFAADHGLLLRRPESLYPLTRRALERGSTQPAVEM
                     VRARRDVARLRAAFDGALAEADVLVAPATPFPAPRFEEEEIDVGSGPPHGLHTGGPAW
                     FTRVVNLVGLPALSLPAGFSDEGLPLGVQLIGRAGEETTLLRLGRAFQRATDHHLREP
                     KARSAEG"
     gene            complement(6905..7363)
                     /locus_tag="VF058_06675"
     CDS             complement(6905..7363)
                     /locus_tag="VF058_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208031.1"
                     /translation="MAGREVLYETFYTKAPGSEKLRRTAEGRFRYLVREGWHEMERRP
                     DGPDAVQIRFEREGASRPLEPLRKKPEPPPRRREGRGGDRGGRGGGFRGGGGPRGGRG
                     GGPPGGQGGPPQGGRSQGGRSQGGQSQGGQSQGGGGGGSPEAPQTQTPSS"
BASE COUNT          958 a         2640 c         2799 g         1045 t
ORIGIN      
        1 gccgaggccc ccgctaggcg atctggtcca tcgtgcagtc ccgcgggtcc ttgtcctcgc
       61 gccaccgcac gaacgtcgcc ccgtgacgga tccgtcgtcc ggtcgtgtgg ttgaaggcga
      121 cctcgcacac gagctcgggg cggagcggga tccacggcgt ctcctcctga cgccaccggc
      181 tcggtccgcc cgggccggcg tgctcgccga acgcgctctc cccgtggtgg ggacggacga
      241 cgtccagcag ctcccggcgt tgcttggccc ggaacgaggc cgtgaagccc acgtggtgga
      301 gcgtgccgtc ctccgcgtag agggccagga gcagcgagcc cacgccctcg ccgtccttgt
      361 ggatccggta gcccccgacc acgcagtcgg cggtcctccg cttcttgacc ttcaccatgg
      421 cccgctcacc cgggcggtac gtgaggtctc cggtcttggc gatcacgccg tcgagcccga
      481 aggctccgag ctcctccatc cagctcgcgg cctcctcggg gtcctcggtc cgaggcgtcc
      541 gggccacgat cggcccgtcg cgcaggaggt cccggatcga tcgctcctcg aggatcccct
      601 ccagcagatc ccaacgctcc gagagagggc ggtcgcagag gtcctcgtcg cccagcgccg
      661 gaaggtcgaa gcccacgaac gaggccggga tcgcctccga gagcatccgc acgcgcgact
      721 cggcggggtg gagccgcagc tgcagggcct cgaagtcgag gcgaccgtcg cgggccagca
      781 cgatctcgcc gtccacgacg caccgctcgg gcagggcctt ccgcaggggg cccagcagct
      841 cggggaagta gcggtgcagg ggccgcttcg tcctgctccc gatgtgaacg tcctccccgt
      901 cccggaagac gatggcccgg aagccgtccc acttcggctc gtagacccag ccctccccgc
      961 gcgggagctc gtcccgggac ttcgacagca tgggcgcgag cggcggcgag atcggcagcg
     1021 tcacgcccgc cacgatagcc ttatcccgac cgatcacaca ggaggaacga agcggtgcca
     1081 cgcgagatcg acatgcagct cttcgagcag gccgtcaacg tgaccgccac cgcgctgcgg
     1141 ggggcgatgg gaggtgagaa gtcccagccg ccgtcctatg ccgccgagct cttccgggag
     1201 gtctgggagg cgctgaagga ggcctcgaaa gacctgccgg accgctccag ggccgggttc
     1261 gggagctagc tactcggcga gccccgcggc cgagaggtcg cggatcgaga cgatccccag
     1321 cacgcggtcg ccctccgaca cggggaggtg acggaacccg ccgtcgagca tcgtcttcag
     1381 cgcctcgctg gcgggcgtct cgggcgggac cgtcgtgggc tctgaggtca tccactcgct
     1441 caccgggcgc tccgcggccc cgaagtcctg accgagtgcg cgcacgatgt cgcgctcggt
     1501 gaagatcccc agcaggcggt cgtcctccat cacgagggcc gatcccaccc tgcgctcgcc
     1561 catcatcgtg gcggcctccg ccactgtggt cgccggcgtg acccggagga cccccgaggt
     1621 catcacatcc ccaacggtct gcatggccgt accttacttc gcccggtgac ctgtgggaac
     1681 cctggcggag gaccccccgt ccaagctccc gggggaccga ggaggtgcgg gatgaaaagg
     1741 ttgtggatcg gcatcgtggc cgcggggctc gtcctggcgg cctgtggagg gggagggacg
     1801 gaggaggccg acggcgccgg ccccgtgccc ggggttgccg gggagcgggc ggccgagggc
     1861 ctccaggtgg aggacgcggc gggcgacgag gccctcgggg acggagccgc cgccgaggcc
     1921 gcggcggccg agctccccgt gttgggcccg tcggtgatca agtcggccca gatcgggctc
     1981 gaggtcgagc gggggggctt caaggacgcg ctccagcggg ccacctcggt ggcgtcggcg
     2041 gaggggggct tcgtcctctc ctcgagcgtg gaggggaagg aggcgcggcg aggctcgatc
     2101 gtgctgcgcg tcccgtctga tcgcttcgag gctgcgctgg cctcgctgcg ggacctgggg
     2161 acggtgcggc gggagcggat cgagggcgag gaggtcggcc aggagttcgt cgatctcgag
     2221 gcgcgcctcc gcaacctgcg ggcgcaggag gcggtgctgc tccgcctgat ggacgaggcc
     2281 ggctcgatcg ccgagacgat ccgggtgcag aggcagctca cggggatcca gctgcagatc
     2341 gagcggatcc gcgggcggct ccgctacctc gaggatcgga cctcgttcgg gacgatctcg
     2401 gtctcgctgg ccgaggttgg ggtcgcggcc ccgagggagc cgagcatcat cgcccgggcc
     2461 tgggagcggg cggccgaggg gttcctctcg gtgatcggcg ggctgatcac tgcgctgggg
     2521 ctgctgacgc cgatcgcggt cctggccctg ctggcgtggc tcctgctccg gcggaggctg
     2581 ccggcgaagg cgtagccgct ccgggacgcc tcgcgtcagc gcagggcggg gaggacgtcg
     2641 gcggcgaaga gctcgatcgc cgcggccgcc gtctcccgat ccatcccggg atagtgcagg
     2701 cgcaggatga ggtgggagtc gcggcgggcc ccgaaagcgt cgacgaacgg gcgcaggtcg
     2761 ccgaccacct ccttgggcgt ccccgccgtg gtgaccgatc gcatcgtctc ctcgtcgggc
     2821 gggtggacct cgaagggccg gtcgggcgtg tcggcccccg ccccccaggc ggggtagacg
     2881 ccgagctgat gggagacgcc ggggaggatc gcctcccagg cgccctcccg ggccacgaag
     2941 gcgttccgga ggatggcgaa gccgaggtcc gacggatcct tccccgcctt gcgcgccccc
     3001 tcctcggccc aggccaggtt gcggtggacg acgtcgagca cggaccgcga cacgatgtag
     3061 ccgtctccga ggcgccccgc gcggcgcacc ggggcctcgg cgaacccccc gaggtagatc
     3121 ggtgggccgc cctcccgggc cggagggggc gtgatcctga cccggtcgta ggagaagacc
     3181 cgaccctcgt ggctgaagcg ctcgcccgtc cacgccttcc ggaggatctc cacggtctcc
     3241 gtcgtgcgac gcaggcgctc gccgatgggg atcccccaca tccggaactc ttcctcgcgc
     3301 cagccgaggc ccagcccgag gatcagccgc ccatcggaga ggaggtccac cgtggccgcg
     3361 tcctcggcca gccgcagcgg gtcgtggaag ggcgcaagca tcacgccggt gcccaggagg
     3421 acgcggtcgg tgacggcggc gagcgcgccg agcacgggga gcagggaggg catgtagccg
     3481 tcgccggcgc catggtgctc gctcacccag accgagtcga agccgacccg ctccgcgagg
     3541 cgcgcgagct cgacgacgtc tcggtattcg tcgacgaacc ctcggcccga gccgggcggc
     3601 acctgcgcgg tgaagagccc gatgccgaac cgcacgtcag cgcctccggg cggcaacctc
     3661 gccgttcgcc gactcccggt cgatcgtccg gtaggacacg ggcgaggaga gcacgatcga
     3721 ggtgatcgtc tccccgtagg gcatcaggcg gtcgatcagg tcctggaggt gcgccaccga
     3781 gcggaccgcg accttggcga tgaaggcctc ctcgccggtg acgcggtggc attcgaggac
     3841 ctcggggaac cccgagatct cggcggccag cgctcggcac tcggcgcccg tcgcggtgat
     3901 gcggatgaac gccagcaccg gcaggccgag ggcctcgcgg tcgacctcga ccctgtagcc
     3961 ccggatcacc ccggcctcct cgaggcggcg cacccggtcg gcgaccgccg gcgcggtgag
     4021 ggcgacccgc ctccccagct cgctgaacgt gatgcgggcg ttctcctgga gctcgcggag
     4081 gatccgccag cccgtctcgt cgagcagccg ctcaggatcc aaggtcatcg cctccattcg
     4141 tcttcgtata acaagcgtcc ccgcccaaag accttagaca gagggttcac aggcggtcaa
     4201 gaccctctct atcatggggt ccatggcacg gcccggctcg atgctctcct cgcctcagga
     4261 ccccacccac ggcggcggac acgcccccac ctgggccatc tggtcggcga tgtgggccgt
     4321 ctacatcgtc tggggctcga cgtacctggc catcgcggtc gtcgtggaga cgtttccgcc
     4381 cttcctctcg gctgcgatcc gcttcctcgt ggccggcgcg ctgctctacg cgtgggcgat
     4441 ccgcaggggg gaccgcgtgg gcgaccgccc tacgctccgc cactggcgga cggcggcgat
     4501 cgtgggcggg gcactgctcc tcggcgggaa cgggatggtg gtgtgggccg agcagacggt
     4561 gccatccggg atcgcggccc tgctcgtcgg caccgtgccg ctgtggatgg tggtcgtctc
     4621 gggcctggtg cttcgtgagc ggacgagctg gcgagaggtg ctggggatcg tcatcgggtt
     4681 cggaggcgtg gccctgctcg tggggccgat cggcgggggc gggaccttcg acccctgggg
     4741 cgtggccgcg gtggtcttcg cctcgatctc gtgggcgtcg ggctcgctct acgcccgccg
     4801 agcgccgctg cccgagcggc cgctcgtggg gacggcgatg gagatgctgg ccgggggggt
     4861 ggtgctcacc gtcgtggccg cgatcaccgg tgagttcggc cgcgtcgact ggggggccat
     4921 ctcactggcg tcgtggatcg gcgttgccta cctcgtggtc ttcggatcgc tggtgggctt
     4981 cgccgcctac gcctggctgc tgcgtgtggc caagacctcg ctcgtcgcca cctacgccta
     5041 cgtgaacccg gtgatcgccg tgctgttggg ctgggctttc ctgcgcgagc cggtcacggc
     5101 gcgcacgctc gtggccgggg tcgtcatcgt gggggcggtg gcgctgatcg tgagcgccag
     5161 ggccgagcag ggcgaggagg agccgtccgg cgagctcggg ggagcctcgg tggagccccc
     5221 cgacgagcag ctcaccgcct gatctcgtcc tcgaactccg ggaagtaccg ggggatcacg
     5281 ctcacgtcga acgtcgagag gatcgactcc ttcggctcgt gctccaggag gaactcgaag
     5341 cacgcggtca cgaagtcctc ccgcgacccg tacccggagc caagccgttc gaagtcctcg
     5401 tccgagaccg tgaccgtgtg cgtggtctgg gtcgatccgt cgtccacgcg cacgaagaag
     5461 gcgtcaccct gcggctccac cgtgatctcg gccacgaccc cacgctagca gggcgcgcgg
     5521 taggcttccc gtcgatgcag gcgaccgagc tcgctcgcgg gatcgccgct cgcgagatct
     5581 cccccgtgga ggtcctggag cgagcgctgc tgcgcgcgga gcactgggac ctcgtctcga
     5641 acgtcttctc gatgctgcac gccgagcagg cgcaggaggt ggcccgcgag cggcaggagg
     5701 aggcggcgcg cggggagttg tggggcccac tccacggcgt gcccgtcgcc gtgaaggacc
     5761 tcttcgacgt cgaggggtgg gagacgaccg ggtgctgccg ggcatatcgg ggcgccgtgg
     5821 ccgagcgcga cgccgcggtg gtggcccggc tccgggcggc cggcgcggtg atcgtcggga
     5881 agaccaacat gcacgagctc gccgcgggcg ggacgaacgc ggtgtcggcg tgcggcgcgt
     5941 gccgcaaccc gtgggacccc tcgaggatca gcggcgggtc gagcgggggc tcggcggccg
     6001 ccgtgggcgc ccgggtcgtg ccgctggcgc tggggaccga cacgggaggc tcggtgcgca
     6061 tccccgcctc catgtgcggc accgtcgggc tgaagaccac gttcgggagc ctcgacatgg
     6121 cgggcgtcat gccgctgacc cgcacgctgg acacggtggg tccgctgacg atgaccgtcg
     6181 aggacgccgc cctggggctc gcggtgatcg cggggcgggg acagcggttc gtggacgaga
     6241 ccgcggcgtc gcccgagggg ctgcgggtgg ccgtcccggg cgccttcttc gccgagcggg
     6301 ctcggcggga cgtcctggcc gcggtgcacg aggcgcgcga cgtcctgatc gggatggggg
     6361 ggaggcccgc cacgggcgag gttggccacg tcggctacgc caacgatgcc tggatcaaca
     6421 tcgcctggcc cgagttcgcc gcggaccacg ggctgctgct ccgccgtccc gagtcgctct
     6481 acccgctcac gcgccgggcc ctggagcgcg gctcgaccca gcccgccgtg gagatggttc
     6541 gagcccggcg ggacgtggcc aggctccgcg ccgcgttcga cggggcgctg gccgaagcgg
     6601 acgtgctggt ggcgccggcc acgcccttcc ccgctccgcg cttcgaggag gaggagatcg
     6661 acgtggggag cggcccgccc cacggcctcc acacgggggg gccggcctgg ttcacccgcg
     6721 tggtgaacct cgtgggcctg ccggccctct cgctccctgc ggggttctcg gacgaggggc
     6781 tgccgctcgg cgtgcagctg atcgggcggg ccggggagga gacgacgctt cttcgtctcg
     6841 gccgcgcgtt ccagcgcgcc accgaccacc acctgcggga gccgaaggcg aggagcgcgg
     6901 agggctagga ggagggggtc tgcgtctggg gcgcctcggg ggacccgccc ccgccaccgc
     6961 cctgagactg cccgccctga gactgcccgc cctgagaccg cccgccctga gaccgcccgc
     7021 cctggggcgg gccgccctgc cctcccgggg ggccgccgcc gcggccgccg cgagggccgc
     7081 cgcccccccg gaacccgccg cctcgaccgc cgcggtcgcc accgcggccc tcgcggcggc
     7141 gcggcggcgg ctcgggcttc ttccgcaggg gctcgagcgg gcgactcgcg ccctcgcgct
     7201 cgaaccggat ctggacggcg tcgggcccgt cgggccgccg ctccatctcg tgccacccct
     7261 cgcggaccag gtagcggaac cgcccctcgg cggtcctccg gagcttctcg gagcccggcg
     7321 ccttggtgta gaaggtctcg tagaggactt ctctgcctgc catgtgggtt cctaacgagg
     7381 ccggacggcc tccggcgggc aactgtagca cacggccgcg ggacctcccg cgcccgagga
     7441 ag
//
LOCUS       DASUTW010000192         3293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_707932, whole genome shotgun sequence.
ACCESSION   DASUTW010000192 DASUTW010000000
VERSION     DASUTW010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707932"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VF058_06680"
     CDS             complement(<1..550)
                     /locus_tag="VF058_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208032.1"
                     /translation="MLGDRDSMFAMARARLSQTQARVGQLEAQVRTLEEHLNLRHAEV
                     EGAAQKIAQLELELDGAREQLREAESLSAELAEAREALGRMNVLSLELAEARERLQDR
                     DRLEGELTEARTNVEDLSRDLDGARRKLREQEQAAEARIASMEAQLAGTRADLEQQAR
                     AAETRATQAEAELAAARAELAAR"
     gene            801..1781
                     /locus_tag="VF058_06685"
     CDS             801..1781
                     /locus_tag="VF058_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6208033.1"
                     /translation="MIVLVTGGTGFIGSNLVRCLLGAGASVRVLDDLSTGRQENLDGL
                     NGGLDLVVGDVRDVEVLASVVRGTEVVYHLAALPSVARSIADPVATHDVNATGTLKVL
                     VSARDAGVRRVVYASSSSVYGDTPILPKHEGMPVAPLSPYAASKLAGEAYCRSFTRSF
                     GLETVALRFFNVFGPRQDPASQYAAVIPRFVNQVLQGESPTVFGDGEQSRDFTYIGNV
                     VQACVLAAEAAAEASGQAMNVGFGRRTTLLELIQILGRIVGREIEPAFAPPRPGDVRD
                     SLADISMAERLIGYRPLMDLRGGLTETVEWFERARRRVAVPARSIEEGER"
     gene            1862..3004
                     /locus_tag="VF058_06690"
     CDS             1862..3004
                     /locus_tag="VF058_06690"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014134790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX6208034.1"
                     /translation="MRPHHANVDRILQDIRGSLEEGVLTNASLVRELERRAAEYLGVR
                     HCIAVSSCTSGLMLALRAAGITGDVIVPSFTFAATAHAVAWNGLRPVFADIDPETLTI
                     SPRAVRRAVGIRTGAILGTHVYGTPCAVDELREIADHWGLRLLFDAAHAFGSRHDGTP
                     VGGFGDAEVFSLSPTKVLVAAEGGIIATNDDALAERCRIGRDYGNPGDYDCLFVGLNA
                     RMSELHAAVALASLEGLDGRIDERNELAAAYRQALGDLSGVTFPTVPDGERSTYKDFT
                     ILVDPGAFGLHPSGLATALEAEGIDTRRYYAPPVHRMQAYRGLADTNGGLPATDLASD
                     RVLTLPLWNGMTEKDVHRVADAVERIQRWVPDRSQVVDLPTEVPGS"
     gene            3001..>3293
                     /locus_tag="VF058_06695"
     CDS             3001..>3293
                     /locus_tag="VF058_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208035.1"
                     /translation="MSDPEETMEAPLTINRAATAIVRSRMHLERAEEELRLALRILTE
                     APVEATALAPSERLARMQRLELYLGRLRELQTMSRTVWDRDGSWWSDASIEGGT"
BASE COUNT          461 a         1162 c         1164 g          506 t
ORIGIN      
        1 gtctcgcggc gagctcggcg cgggccgcgg ccagctccgc ctcggcctga gtcgctcggg
       61 tctcggcggc ccgggcctgc tgctccaggt cggcgcgggt gccggcgagc tgggcctcca
      121 tcgaggcgat ccgggcctca gcggcctgct cctgctcccg cagcttgcga cgggccccat
      181 ccagatcacg ggaaaggtcc tcgacgttgg tgcgagcctc cgtcagctcg ccctcgagcc
      241 ggtcgcgatc ctggagccgc tctcgggcct ccgcgagctc gagggagagg acgttcatcc
      301 ggcccagggc ctcgcgggcc tcggccagct cggccgagag cgactcggcc tcgcggagct
      361 gctcgcgggc gccgtcgagc tccagctcga gctgcgcgat cttctgggcg gcgccctcga
      421 cctcggcgtg gcgaaggttg aggtgctcct ccaacgtcct gacctgggcc tcgagctggc
      481 cgacgcgggc ttgggtctgg ctcaggcggg cccgagccat ggcgaacatg ctgtccctgt
      541 cgcccagcat gcggcgcacg ctcgagcgtg agtagccgaa gagtcctcgc ttcagacgag
      601 gaggctggtt ctgcgtcata gctcttcccc ccggggaccg ctgaaccatt atgggcacca
      661 gccccaaggt tgcaacgacc cctgggggac gccctgagag cctacgacat ctgtggtagc
      721 cgaacctacg gtccctgaag cgtgggcgtg ggtccatatc gccgtacctt cgaggacatg
      781 caggagggga gctgggtgca gtgatcgtgc tcgtgacggg tgggacgggc ttcatcggat
      841 cgaacctggt gcggtgcctg ctgggcgcag gcgcctccgt gcgggtgctg gacgacctct
      901 ccacggggcg gcaggagaac ctcgacggcc tcaacggggg cctcgacctc gtcgtggggg
      961 acgtgaggga cgtcgaggtg ctggcgtccg tggtccgcgg caccgaggtt gtctaccacc
     1021 tcgccgcgct cccctcggtg gcccggtcga tcgccgatcc ggtcgccacc cacgacgtga
     1081 acgcgacagg cacgctgaag gtcctggtct cggcgaggga cgccggggtg cggcgcgtcg
     1141 tctatgcatc ctcgtcctcc gtctacgggg acacaccgat cctgcccaag cacgagggga
     1201 tgccggtcgc cccactgtcc ccgtacgcgg cttccaagct cgccggcgag gcctactgcc
     1261 ggtccttcac ccgctccttc ggcctcgaga ccgtggcgct ccgcttcttc aacgtgttcg
     1321 gcccgcgcca ggacccggct tcgcagtacg cggccgtgat ccctcggttc gtgaatcagg
     1381 tcctccaggg ggagtccccc accgtgttcg gcgacggcga gcagagccgg gacttcacct
     1441 acatcgggaa cgttgttcag gcgtgcgtgc tcgccgccga ggccgccgcc gaggcctcgg
     1501 gtcaggcgat gaacgtgggg ttcggccgcc gcacgacgct cctggagctg atccagatcc
     1561 tgggacggat cgtcggccgg gagatcgagc ccgccttcgc cccgcccagg cccggcgacg
     1621 tccgggactc gctggccgac atctcgatgg cagagcgcct catcggatac cgaccgctga
     1681 tggaccttcg cggcggcctg accgagaccg tggagtggtt cgagcgggcg cgacgtcgcg
     1741 ttgccgtccc cgctcgttcg atagaggagg gagagcgatg accgttgaac ttccccggcc
     1801 ggagccggcg cgaccggcga tagcgggagg tccccccgcc ttccccgagg gacttcccct
     1861 ggtgcgcccc caccatgcca acgtcgaccg gatcctgcag gacatccgtg ggagcctcga
     1921 ggagggcgtc ctgacgaacg cgtccctggt tcgggagctc gagcggcggg cggccgagta
     1981 ccttggggtt cgccactgca tcgcggtctc ctcgtgcacg agcggcctga tgcttgcgct
     2041 tcgggcggcg ggcatcacgg gcgacgtgat cgtcccctcc ttcacgttcg cggccacggc
     2101 gcacgcggtg gcctggaacg gcctccgtcc cgtgttcgcc gacatcgatc ccgagacgct
     2161 cacgatctcg ccccgcgcgg tgcggcgggc ggtcgggatc cgaaccgggg cgatcctcgg
     2221 cacccacgtc tacggaacgc cgtgcgccgt cgacgagctg cgcgagatcg ccgaccactg
     2281 gggcctccgg ctcctcttcg acgccgccca cgcgttcggg tcacggcacg acggaacacc
     2341 ggtgggcggc ttcggcgacg ccgaggtctt cagcctgtcg cccacgaagg tgctggtggc
     2401 ggcggaaggg ggcatcatcg ccacgaacga cgacgccctc gccgagcggt gcaggatcgg
     2461 acgggactac ggcaatccgg gcgactacga ctgcctcttc gtgggcctca acgccaggat
     2521 gtccgagctc cacgcggccg tggcgctggc ctcgctggaa gggctcgacg gccggatcga
     2581 cgagcggaac gagctcgcgg cagcctaccg ccaggccctc ggcgacctct cgggcgtcac
     2641 cttcccgacg gtgccggacg gcgaacggag cacctacaag gacttcacca tcctcgtgga
     2701 ccccggggcg ttcggtctcc atccctcggg cctggccacg gccctcgagg cggaggggat
     2761 cgacaccagg aggtactacg cgccgcccgt gcaccgcatg caggcgtacc ggggcctcgc
     2821 cgacacgaac ggcggccttc ccgcgaccga cctggccagc gaccgcgtcc tgaccctccc
     2881 gctgtggaac gggatgacgg agaaggacgt gcaccgggtg gcggacgccg tggagcgcat
     2941 ccagcgctgg gtcccggacc gctcgcaggt cgtcgacctg ccaacggagg ttccgggctc
     3001 atgagcgatc cggaggagac catggaggcc cccctgacca tcaatcgggc agcgacggcc
     3061 atcgtccgga gcaggatgca cctggagagg gccgaagagg agcttcggct cgctctgcgg
     3121 atcctcaccg aggcccccgt cgaagccacg gccctcgccc cgtccgagcg tctggctcgg
     3181 atgcagcgcc tcgagctcta cttgggcaga ttgcgtgagc tgcagacgat gtcgcgaacc
     3241 gtgtgggatc gggacggatc gtggtggtcg gacgcctcga tcgagggggg aac
//
LOCUS       DASUTW010000193         2461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_837432, whole genome shotgun sequence.
ACCESSION   DASUTW010000193 DASUTW010000000
VERSION     DASUTW010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837432"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VF058_06700"
     CDS             <1..291
                     /locus_tag="VF058_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208036.1"
                     /translation="RYPAQALLVVAVGMSVPMIPWMRFRGHSTRSSYEMAAAMAVPVI
                     PFVCLALFGVVEGAQCGLYCASGFVAMLGLMLYRRAEYISHGGSPHAVSPTG"
     gene            653..1072
                     /locus_tag="VF058_06705"
     CDS             653..1072
                     /locus_tag="VF058_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208037.1"
                     /translation="MRLTWRDGIATLFAGAVGIVYGLWLTETAMSGMSTRVLGGIVLG
                     LGMLGCAANQAALADVFGVGGKRRAPMAYVVFMSAAGGVALVAGAIAVVAGSKTLLTT
                     LVIATLTLWLITTVRHAVTPPTHPAEAPAGRPMAKVA"
     gene            complement(1430..1864)
                     /locus_tag="VF058_06710"
     CDS             complement(1430..1864)
                     /locus_tag="VF058_06710"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6208038.1"
                     /translation="MARFEEHVDVGTSVEETFRYITDQKQVASWNDHVQRVEVDGGGP
                     VAPGTIIRQHRRRGNREFVLTFQVIDHESPRRHALRGEVFGTSTTMEFGLEPRGSGTR
                     VSMAATVEARGLKGVLAPLVARQMRKSTVAALTALRERLGAA"
     gene            complement(2092..>2461)
                     /locus_tag="VF058_06715"
     CDS             complement(2092..>2461)
                     /locus_tag="VF058_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX6208039.1"
                     /translation="FSPGPVTIKSGETITWEHADKTEAPHTVTIATQEQLVQDFGDFL
                     GGCPECDAAAGAALDGHFGAGPPVFELDDGDGEFDDAGDSLLFFPGDSVSKQINAPAG
                     TTLLYFCAIHSWMQGAISVN"
BASE COUNT          356 a          806 c          870 g          429 t
ORIGIN      
        1 cgatacccag ctcaggcgtt gctcgtggtg gctgtgggca tgagtgtgcc gatgatcccg
       61 tggatgcggt tccggggcca cagtacgagg agctcgtacg agatggcggc ggccatggcc
      121 gttcccgtga ttccgttcgt ctgcctggcc ctgttcggtg tcgtggaagg agcacagtgc
      181 ggcctttact gcgcttcggg gttcgtggcg atgctcggcc tcatgctgta ccggcgggcc
      241 gaatacatct cacatggtgg atccccccac gcagtgagcc ccacaggata gacccggcga
      301 gcggtggcac gctgagtcga catgccgtac gcgcggccca tcctcgccat ggtggacagc
      361 cgggggcggg tcgtggaacc tgtggcacgg tgcggggctt cagtcttctg gtgcaccgca
      421 ccgccagccc gctgttatcc gaggtgcgcg aggtcctctc gctttcggtc cgacgggccc
      481 gctggggccg ctcaggagcg cgacggtcag cgtgccctcc atgtcggagg tccttggcgg
      541 tcggggccgg atgcctctgc cccggagatg gctacggtgc gaggctataa gcagccgccg
      601 tggcaggcaa gcgggtcgtg gccgccccgg cgggacggaa aggagtagga cgatgcgcct
      661 tacgtggaga gacgggatcg cgactctgtt cgcgggcgcg gtcgggatcg tctacggcct
      721 gtggctcacg gagacggcca tgtccggcat gtcgaccagg gtgctgggtg ggatcgtcct
      781 tgggctgggg atgctgggat gcgccgcgaa ccaggccgct ctggcggacg tcttcggcgt
      841 tggtgggaag cgacgggccc ccatggccta tgtcgtattc atgtccgcgg ctggaggcgt
      901 cgccctggtt gctggggcca tcgccgtggt ggccggcagc aagacgctgc tgactacgct
      961 cgtgatcgcc accctgacgc tctggctgat cacgacggtc cgccacgccg tgacccctcc
     1021 cacccatccg gcggaagcgc cggccgggag gccgatggcg aaggtcgcct agcagaacgg
     1081 agaggcatcg gagctcggcg acgcctggcg atcccaggtc gaggctgggg ccgtcccctc
     1141 ccgaccgccg gagagggtcg ccgggaccct gggccgggcc tgggcgtgga tcctgggtgc
     1201 ggtgcccgcg ggtcgggggg atctttcccg tggtggccgg tcccgtgctg ggacctctcc
     1261 ggtcttggct tcggactggg agcggcgtta gctcgacccc aggcgtggct cttggcagtt
     1321 gcggttgcac cgctcactag attgcccggc cattggatcg ctgaggcttc ggggatggtg
     1381 cgatgtcaat gctgacagcc ggcttcgccc cccggccgtg gcacgaggac tacgctgcgc
     1441 cgagtcgctc ccgcaacgcg gtcagcgccg cgacggtcga cttgcgcatt tgccgggcga
     1501 cgagcggcgc caggactccc ttgagacctc gagcttcgac ggtcgcggcc atggacaccc
     1561 gagtgcccga tcctcgtggt tccaagccga actccatcgt cgtgctcgtc ccgaacacct
     1621 ccccccgaag cgcgtggcgc cgaggggatt cgtggtcgat aacctggaag gtcaggacga
     1681 actcgcggtt ccctcgccgg cggtgctggc gaatgatagt tccgggcgcg acagggccac
     1741 caccgtcaac ctcgacccgc tggacgtgat cgttccagct cgccacctgc ttctggtcgg
     1801 tgatgtaccg gaacgtctcc tcgaccgagg tgcccacgtc cacgtgctct tcgaaacgcg
     1861 ccaagccgca tcccctcgat cggaggccgc ggaacccatc gcaaggcaat ggctccccag
     1921 agtggcaggg gaaggagccg accgcaaggg acttcgatca ccctccctgg aggcgcaacg
     1981 aagcaccggc gtcatcccga gttcgaagag aagttcgagc gcggggcgac gatcaccatg
     2041 cggccccggg ccggatcatg tcgctgatgt tcgccgcctc gctcgactcc tttaattgac
     2101 cgagatggcc ccctgcatcc aggagtggat cgcgcagaag tacaggagcg tggtcccggc
     2161 gggcgcgttg atctgcttgc tcaccgagtc gcccgggaag aacaggagcg agtcgcccgc
     2221 atcgtcgaac tcgccgtcac catcgtccag ctcgaagacc ggcggtccgg cgccgaagtg
     2281 gccgtcgagc gctgcacccg ctgcggcgtc gcactccggg caccctccga ggaagtcccc
     2341 gaagtcctgc acgagctgtt cctgggtggc tatcgtgacc gtgtggggcg cctcggtctt
     2401 atcggcgtgc tcccaggtga tcgtctcacc gctcttgatg gtcaccgggc ccggcgagaa
     2461 g
//
LOCUS       DASUTW010000194         8343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1096412, whole genome shotgun sequence.
ACCESSION   DASUTW010000194 DASUTW010000000
VERSION     DASUTW010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8343
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096412"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VF058_06720"
     CDS             <1..301
                     /locus_tag="VF058_06720"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX6208040.1"
                     /translation="PHDRVRADVREARLGVRPEKIRIAPGESAPPEGRNAIRGRLLVS
                     TYVGVSSVYTVEAPGGEQLTVYAQNVDAPGERAPATGEEVVLSWHPQHTFVVEPD"
     gene            313..1557
                     /locus_tag="VF058_06725"
     CDS             313..1557
                     /locus_tag="VF058_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX6208041.1"
                     /translation="MTERMNELFGGLQDPMSRRELLRRAGRGSVFLGVGALLAACGIQ
                     GEDDDAGPAPTEASPSPLPEIAGQVVVANWPLYIDRAKGESPTLVSFTEETDIQIDYQ
                     VVINDNEEFFGTIQEPLSRGQSAGYDIIVVTDWMVAKMIRLGFLQPLHHDRLPNFEAN
                     AFEKFKDPSYDPGNIYSIPWAAGITGIGYDIERTGRELTSREDLWDPAFEGHVGMFTE
                     MRDSFGFALLANGVVPEEATMEDVEAAQQKLLEQRETGIVRGYFGNDYTDQLATGNLW
                     VSMAWSGDIFALQQDNPNLRFIIPEEGAMRWTDNMVIPVGAEHPADAHEFMNFVYDPR
                     IATNITEWVWYESPVSQVPDLIRQDAAEASGGEAELLDQIAESPLVFPSEEVESQLYN
                     YKNLDEEDERAWNELFQAVVQG"
     gene            1562..2446
                     /locus_tag="VF058_06730"
     CDS             1562..2446
                     /locus_tag="VF058_06730"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011607869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6208042.1"
                     /translation="MTTTVETAAPRSARWRRAGPPYSLLLPGGLWLLVFFVVPMVSML
                     SVSLQEGSLEQGYELTWRFQTYLDVLGKFDRQFIRSIFYGLMVTGALLLICYPLAYTI
                     AFRGGRWKNALLFLLIMPFFVSFIIRTLEWKFILADDGFIFGPLKNLGWLGQDFRVLA
                     TPFAVIGGLVYNFLPFMALPLYVALERMDRSLLDAAGDLYSSRTSAFLRVTLPLSLPG
                     VFAGSLLTFIPAVGDFINAEVLGGINTTMIGNVIQNEFLVNNDYPEAAAISFLLLAGI
                     LIGVAVYAKLLGTEELTG"
     gene            2443..3315
                     /locus_tag="VF058_06735"
     CDS             2443..3315
                     /locus_tag="VF058_06735"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6208043.1"
                     /translation="MSVRPPLRDVVAPPGATETPVRRPERRGWILPAYTGLVIVYLVL
                     PILVMIVFGFNDITGRFNFTWQVFTLEHYRTLGDFPDLIEAIKNSLLIAAGATIVSTV
                     LGTMMALALTRYRFRGRAGANLFIFLPLGTPEVVLGASLLSLFITLGMDRGLLTILIA
                     HIMFNISFVVVTVKARTQGFNREWEEAAKDLGASPWTAFRTVTLPLIFPGVLAAAVLA
                     FALSVDDFVITLFNAGGELTFPLWVYGASRFGVPPQVNVMGTLIFVIGVALALGSVWL
                     QRRRGEEIVAGYLR"
     gene            3325..4680
                     /locus_tag="VF058_06740"
     CDS             3325..4680
                     /locus_tag="VF058_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX6208044.1"
                     /translation="MDDRITARAREIAKEETARLLERTPASAELYERASRSMPMGVAS
                     SFHAAEPYPVYLEGGRGSRVWDVDETEYIDFHNGFGTMAVGHAHPKVVEAIRRTAGEG
                     THFAVTTETAIAFAEELRRRFQLDLVRFVNSGTEATMDAIRIARAATGRSTVLKIAGS
                     YHGHHDTVMFSVVSNADMMGGRDVPPDRPMSKGIPPEMAERTLVVPFNDADALETLMD
                     ERGSEIACLIMEPVMMNIGIAVPQPGYLERVRELCTRHGVVLIFDEVKSGATIAEGGA
                     TERFGVQPDLACFAKAIGGGVPTGAFGGKADVMDVINHGAAQQGTFNGNPLAAAAGLA
                     ALTEVLTPDAYEQMGAIGTRLAEGCRAAIEEHGIPAHTVDLGCKGCVSYRRKPLRDYR
                     DFLEANGELFEASWAWAMNRGIFMTPGDEEQWTLSVQHSEEDVDRFVEVFAEFCAAVA
                     S"
     gene            4677..5498
                     /locus_tag="VF058_06745"
     CDS             4677..5498
                     /locus_tag="VF058_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6208045.1"
                     /translation="MSDPVLLEEQLGPVRRLTLNRPDALNALSSELIEELSAALGRAA
                     EDDDARVVVVRGAGRAFCAGYDLKEEGRSGEMDAVAWRNELAHDLRRMLEIFDHPRPV
                     IAEVHGYCLAGGCDLMMMCDVAVASDDAMFGEPEIRYGSGVVTLVMPWLVGARKAKEL
                     LLTGEDRIPADEALRIGLVNRVVPRDRLEEETLELAREIAVMDPVSISLTKAAINRSL
                     EAAGFREALWANLDLDALIEAAEVPERKEFNRIREEEGLKAAIRWRDDRFRTEDA"
     gene            5495..7591
                     /locus_tag="VF058_06750"
     CDS             5495..7591
                     /locus_tag="VF058_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX6208046.1"
                     /translation="MTSARALRPLLAPRSIAIVGASEAEGSWAPEFYENLRAAGFEGP
                     IFPVNPKYREVWGFRCVPTAADLPEGVDLAVIVVPARHAVSAAEDAARAGVRSAMVVS
                     SGFAEAGGEGAAMQEALAAAARRAGMPVVGPNVEGYVNVTDRVWCYGTGRPPSLVPGG
                     LTIVSQSGTVAWSLAQMAADRALGVRLVMGVGNEASVGLADMLAWAAADPETTVVACY
                     VETVRDPAGFAKAAEALAGAEKPLLVCAPGHGEIAGRAVVAHTGAVAGRTGVRDAWLR
                     RLGAALVPDSVALFEAAALASRATPPKRRGVAVAFQSGGSCTLFAEHAEAAGLEMPPP
                     SAATRRRLRRALPPFAKPDNPLDVTGQAAVETGMFEAALEALARDPAIGVVAFDAFPS
                     RDPVQGSWEPSVLAAAERLAKETGTVMVSLAPVPLALDDAGRKSAEHLRLPVLQGIQT
                     APTAIRALLDLSETPGGRAQAPHPARRRAAALLRGRSGALDEVRARKLLGLYGIEAPD
                     EAVAHTPQEAAPAAARLGFPVAVKALAAALPHKARAGAVKVGLRTGPDVERAAAAVLR
                     AARRAGVEPEGVLVQRMASGAEVLVGGLWDERFGPAVTVKPGGAAAEAGEAEFLAAPL
                     RSGQAAAFVGRRAARLGLERRRHDLRAVAAALDAVARLLVDLKGRLVEIEANPLIVSR
                     RGAVAVDALAVVSERA"
     gene            complement(7575..8039)
                     /locus_tag="VF058_06755"
     CDS             complement(7575..8039)
                     /locus_tag="VF058_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEX6208047.1"
                     /translation="MFEKISDPREVRLVEGHIPLQHRYTPGVAGEAFFASLMKGEFLA
                     SGCERCGIVYCPNRIFCERCMGELEADRTVGPRGRIESFTVLYAGVEGEPLDEPRTIG
                     LVRLEGADTVLMHELVGFDEGPQIGDEVEPDFEPRSRRTGSILDLRGFRPAR"
     gene            complement(8039..>8343)
                     /locus_tag="VF058_06760"
     CDS             complement(8039..>8343)
                     /locus_tag="VF058_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208048.1"
                     /translation="DMVELEEPELPFVLELDVDTPMMGFMHKLGEVDPDRIEVGMAVE
                     AVWKPEEEREGSILDIRYFRPRDAALRKGAKRKPAAKRTSKRSGSRKRSGAKRRAG"
BASE COUNT         1154 a         2906 c         3043 g         1240 t
ORIGIN      
        1 gccccacgac cgcgtgcgcg ccgacgtccg cgaggcccgc ctcggcgtcc gtcccgagaa
       61 gatccgcatc gcccccggcg agtcggcccc gcccgagggc cgcaacgcga tccgggggcg
      121 gctgctcgtc tcgacgtacg tgggcgtcag cagcgtctac acggtggagg cgcctggcgg
      181 cgagcagctg accgtgtacg cgcagaacgt cgacgcgccc ggggagcggg cgcccgccac
      241 gggggaggag gtcgtcctct cctggcaccc gcagcacacg ttcgtggtgg agccagactg
      301 aaggaggtcg gaatgacgga gaggatgaac gagctgttcg gcggtcttca ggacccgatg
      361 tcgcgacggg agctcctgcg tcgcgcgggc cgcggatcgg tgttcctggg cgtgggcgcg
      421 ctcctggccg cctgcggcat ccagggcgag gacgacgatg ccggcccggc gcccaccgag
      481 gccagcccct cgccgctgcc cgagatcgcg gggcaggtcg tggttgccaa ctggcccctc
      541 tacatcgacc gggccaaggg cgagagcccc acgctcgtgt ccttcaccga ggagacggac
      601 atccagatcg actaccaggt ggtcatcaac gacaacgagg agttcttcgg gacgatccag
      661 gagcccctct cccgcgggca gtcggccggc tacgacatca tcgtggtcac cgactggatg
      721 gtggccaaga tgatccggct ggggttcctc cagccgctgc accacgaccg cctgcccaac
      781 ttcgaggcga acgccttcga gaagttcaag gacccctcct acgaccccgg gaacatctac
      841 agcatcccgt gggcggccgg gatcacgggg atcggctacg acatcgagcg gaccggcagg
      901 gagctgacct cgagggagga cctgtgggac cccgcgttcg aggggcacgt ggggatgttc
      961 accgagatgc gggactcctt cgggttcgcc ctcctcgcga acggcgtggt ccccgaggag
     1021 gccacgatgg aggacgtgga ggccgcgcag cagaagctgc tcgagcagcg tgagaccggc
     1081 atcgtccgcg gctacttcgg caacgactac acggaccagc tcgccacggg gaacctctgg
     1141 gtctcgatgg cgtggtcggg ggacatcttc gccctccagc aggacaaccc gaacctccgg
     1201 ttcatcatcc ccgaggaggg ggcgatgcgc tggaccgaca acatggtgat cccggtcggg
     1261 gccgagcacc cggccgacgc gcacgagttc atgaacttcg tgtacgaccc gcgcatcgcc
     1321 acgaacatca cggagtgggt ctggtacgag tcgcccgtga gccaggtgcc cgacctcatc
     1381 cgccaggacg cggcggaggc ttcgggcggc gaggcggagc tgctggatca gatcgccgag
     1441 agcccgctcg tcttcccctc ggaggaggtc gagtcccagc tctacaacta caagaacctc
     1501 gacgaggaag acgagcgggc ctggaacgag ctcttccagg cggtggtcca gggctgaggg
     1561 ggtgacgacg acggtcgaga cggcggcgcc gcggtcggcg aggtggcgca gggcgggccc
     1621 gccctactcc ctgctcctcc ccggtgggct gtggctgctc gtcttcttcg tggtgccgat
     1681 ggtgtcgatg ctgtcggtct cgctgcagga ggggtcgctg gagcaggggt acgagctgac
     1741 ctggcgcttc cagacctacc tcgacgtcct ggggaagttc gacaggcagt tcatccgatc
     1801 gatcttctac gggctcatgg tgacgggggc actgctcctg atctgctacc cgctcgcata
     1861 cacgatcgcg ttccggggcg gccgatggaa gaacgccctg ctgttcctcc tgatcatgcc
     1921 gttcttcgtc tcgttcatca tccggacgct ggagtggaag ttcatcctgg ccgacgacgg
     1981 gttcatcttc ggcccgctga agaacctggg gtggctgggg caggacttcc gcgtcctggc
     2041 gacgcccttc gccgtgatcg gcggcctcgt ctacaacttc ctgcccttca tggcgctccc
     2101 gctctacgtg gcgctggagc ggatggatcg gagcctgctg gacgccgccg gggacctgta
     2161 ctcgagccgc acgtcggcgt tcctccgcgt gacgttgccc ctgtcgctgc cgggggtgtt
     2221 cgcgggatcg ctcctgacgt tcatccccgc cgtgggcgac ttcatcaacg ccgaggtcct
     2281 gggcgggatc aacacgacga tgatcggtaa cgtgatccag aacgagttcc tcgtgaacaa
     2341 cgactacccg gaggcggcgg cgatctcgtt cctgctgctg gcggggatcc tgatcggggt
     2401 ggcggtctac gccaagctcc tcggcaccga ggagctgacg ggatgagcgt gagacctccg
     2461 ctcagggacg tggtggcgcc gccgggggcc accgagacgc cggtccggag gccggagcgc
     2521 cggggctgga tcctgcccgc gtacaccggt ctggtgatcg tctacctcgt cctgcccatc
     2581 ctcgtgatga tcgtgttcgg cttcaacgac atcacgggac gcttcaactt cacgtggcag
     2641 gtgttcacgc tcgagcacta ccgcacgctg ggcgacttcc ccgacctcat cgaggcgatc
     2701 aagaactcgt tgctgatcgc tgcgggggcc acgatcgtct ccacggtgct gggcacgatg
     2761 atggccctgg ccctgacccg ctaccgcttc cgggggaggg ccggggcgaa cctgttcatc
     2821 ttcctgccgc tgggcacgcc cgaggtcgtg ctcggcgcct cgctgctctc gctgttcatc
     2881 acgctgggga tggaccgagg cctgctcacg atcctcatcg cgcacatcat gttcaacatc
     2941 tcgttcgtgg tcgtgaccgt gaaggctcgg acccagggct tcaaccggga gtgggaggaa
     3001 gcggcgaagg acctcggggc ctccccctgg acggcgttcc gcaccgtgac cctgccgctg
     3061 atcttcccgg gggtgctggc cgccgcggtg ctggcgttcg cgctctcggt cgacgacttc
     3121 gtgatcacgc tgttcaacgc gggcggcgag ctcacgttcc cgctctgggt gtacggggcc
     3181 agccgcttcg gggttccgcc ccaggtcaac gtgatgggta ccctgatctt cgtgatcggc
     3241 gtcgcgctcg cgctcgggag cgtctggctg cagcggcgcc ggggcgagga gatcgtcgcc
     3301 ggctatctgc gataaggagg agcgatggac gaccggatca ccgcacgcgc tcgggagatc
     3361 gccaaggagg agaccgcgcg cctcctggag cggacgccgg catccgccga gctgtacgag
     3421 cgagcgtcga ggtcgatgcc gatgggcgtg gcgtcctcct tccacgccgc cgagccctac
     3481 cccgtctacc tggagggagg gaggggcagc cgcgtctggg acgtggacga gaccgagtac
     3541 atcgacttcc acaacggctt cgggacgatg gccgtgggtc acgctcatcc caaggtcgtg
     3601 gaggcgatcc gccggacggc cggcgagggc acccacttcg cggtgaccac cgagaccgcg
     3661 atcgccttcg ccgaggagct gcgccgccgg ttccagctcg acctggtccg cttcgtgaac
     3721 tcggggaccg aggccacgat ggacgcgatc aggatcgccc gggccgccac ggggcggagc
     3781 accgtcctga agatcgccgg gtcctaccac gggcaccacg acacggtgat gttcagcgtc
     3841 gtgtccaatg ccgacatgat gggcggccgc gacgtcccgc ccgaccggcc gatgtcgaag
     3901 ggcatccccc ccgagatggc cgagcggacg ctcgtggtgc cgttcaacga cgcggacgcg
     3961 ctggagaccc tgatggacga gcggggttcc gagatcgcct gcctgatcat ggagccggtg
     4021 atgatgaaca tcggcatcgc cgtcccccag cccggctacc tggagcgggt gcgggagctc
     4081 tgcacccgcc acggcgtggt cctgatcttc gatgaggtga agtctggggc cacgatcgcc
     4141 gagggcggcg ccaccgagcg gttcggtgtc cagcccgacc tggcctgctt cgccaaggcg
     4201 atcggtggcg gcgtcccgac cggcgcgttc gggggcaagg ccgacgtcat ggacgtgatc
     4261 aaccacggcg cggcccagca ggggacgttc aacggcaacc cgctggcggc cgcggcgggc
     4321 ctcgcggccc tcaccgaggt cctcaccccc gatgcctacg agcagatggg agcgatcggg
     4381 acccggctgg ccgaggggtg ccgggccgcg atcgaggagc acggcatccc cgcgcacacc
     4441 gtggacctgg ggtgcaaggg gtgcgtctcc taccggcgga agcccctgcg cgactaccgc
     4501 gacttcttgg aggcgaacgg cgagctgttc gaggcctcgt gggcctgggc catgaaccgc
     4561 gggatcttca tgacgccggg cgacgaggag cagtggacgc tctcggtcca gcactccgag
     4621 gaggacgtgg accgcttcgt cgaggtcttc gccgagttct gcgccgcggt cgcgtcgtga
     4681 gcgacccagt cctgctggag gagcagctcg ggccggtccg ccgcctcacc ctgaaccgcc
     4741 cggacgcgct gaacgcgctg tcgagcgagc tcatcgagga gctgtcggcg gcgctggggc
     4801 gggccgccga ggacgatgac gcccgggtgg tggtggtccg gggagcgggg agggcgttct
     4861 gcgccggcta cgacctcaag gaagagggtc ggtcgggcga gatggacgcg gtcgcctggc
     4921 ggaacgagct ggcccacgac ctccggcgga tgctcgagat cttcgaccac ccccggccgg
     4981 tgatcgcgga ggtgcacggc tactgcctcg cgggcggctg cgacctcatg atgatgtgcg
     5041 acgtcgcggt ggcgtcggac gacgcgatgt tcggggagcc ggagatccgc tacggcagcg
     5101 gcgtggtcac cctcgtcatg ccctggctcg tcggcgcgcg caaggccaag gagctgctgc
     5161 tgaccggcga ggaccggatc cccgccgacg aggccctccg gatcgggctc gtcaatcggg
     5221 tcgtgccgcg cgaccggctg gaggaggaga cgctcgagct ggcccgcgag atcgcggtga
     5281 tggatccggt cagcatctcc ctgacgaagg ccgcgatcaa ccgctcactc gaggccgcgg
     5341 ggttccgcga ggcgctctgg gccaacctcg atctggacgc gctgatcgag gcggcggagg
     5401 tgcccgagcg caaggagttc aaccgtatcc gggaggagga ggggctgaag gccgcgatcc
     5461 gctggcgcga cgaccgcttc cgcacggagg acgcgtgacg agcgcgcggg ccctgcgccc
     5521 gctgcttgcc cctcggtcga tcgcgatcgt aggggcctcg gaggccgagg gctcctgggc
     5581 ccccgagttc tacgagaacc tgcgggcggc ggggttcgag ggaccgatct tccccgtcaa
     5641 cccgaagtac cgcgaggtgt gggggttccg gtgcgtgccc acggcggccg acctgccgga
     5701 gggggtcgac ctcgcggtga tcgtggtccc cgcgcgccac gccgtttcgg cggcggagga
     5761 cgcggcgcgg gcgggcgtcc gcagcgccat ggtcgtctcg agcgggttcg ccgaggcggg
     5821 gggcgagggc gcggcgatgc aggaggcact ggcggcggcg gcgcggcggg ccggcatgcc
     5881 ggtggtcggg ccgaacgtcg agggctacgt caacgtcacc gaccgcgtct ggtgctacgg
     5941 gacgggacgg ccgccgtccc tcgtgccggg gggtctcacg atcgtctcgc agagcgggac
     6001 ggtggcctgg tccctcgccc agatggccgc cgaccgggct ctcggggtcc gcctcgtgat
     6061 gggggtgggg aacgaggcct cggtcgggct ggccgacatg ctggcctggg ccgccgccga
     6121 cccggagacg acggtcgtcg cctgctacgt cgagaccgtg cgggatcccg ccgggttcgc
     6181 caaggccgcg gaggccctcg cgggggcgga gaagcccctg ctcgtgtgtg cgcccggcca
     6241 cggcgagatc gccggtcgcg cggtggtggc ccacacgggc gccgtggccg ggcgcaccgg
     6301 cgttcgcgac gcgtggctgc gccgcctcgg agccgccctc gtcccggact cggtggcgct
     6361 cttcgaggcc gcggccctcg cgtcccgcgc caccccgccg aagcgccggg gcgtcgcggt
     6421 cgcgttccag tcgggcggca gctgcacgct gttcgccgag cacgcggagg ccgcgggcct
     6481 cgagatgccg ccgccgtccg cagccacccg ccggcggctg cggcgagccc tccctccctt
     6541 cgccaagccc gacaatcctc tcgacgtcac ggggcaggcc gcggtggaga ccgggatgtt
     6601 cgaggccgcc ctggaggccc tggcccgcga ccctgcgatc ggggtcgtgg cgttcgacgc
     6661 cttcccctcc cgcgaccccg tccaggggag ctgggagccc tcggtcctgg ccgccgcgga
     6721 gcggctggcg aaggagacgg gaaccgtgat ggtctcgctt gcccccgtcc cgctggccct
     6781 cgacgacgcg ggtcggaagt ccgcggagca cctccggctc cccgtcctgc agggcatcca
     6841 gacggcgccg acggcgatcc gggccctgct ggacctctcc gagaccccgg gaggccgcgc
     6901 ccaggccccg cacccggcgc gccgtcgggc cgccgccctg ctgcggggac ggtccggggc
     6961 tctggacgag gtccgggcca ggaagctgct gggactgtac gggatcgagg ccccggacga
     7021 agccgtcgcc cacacccccc aggaggccgc cccggccgcg gccaggctgg gtttccccgt
     7081 ggccgtgaag gcgctggccg cggccctgcc gcacaaggcg cgcgcgggag cggtgaaggt
     7141 cgggctccgc accgggccgg acgtcgagcg ggcggccgcc gcggtcctcc gggccgcccg
     7201 gcgcgcgggc gtggagcccg agggcgtcct ggttcagcgc atggcctcgg gggccgaggt
     7261 cctggtgggc gggctctggg acgagcgctt cggcccggcc gtcaccgtga agccgggcgg
     7321 cgcggcggcc gaggcgggcg aggcggagtt cctggcggcg ccgctccggt cgggccaggc
     7381 cgcggccttc gtcggccgcc gcgccgctcg gctggggctc gagcgccgcc gccacgacct
     7441 ccgggccgtg gcggcggcgc tcgacgcggt ggcccgcctc ctcgtcgacc tcaagggacg
     7501 gctcgtggag atcgaggcga accctctgat cgtgtcgcga cgcggagcgg tggcggtgga
     7561 cgccctggcg gtggtcagcg agcgggcctg aagccccgga ggtcgaggat ggagcccgtc
     7621 cgccggctcc tcggctcgaa gtcgggctcg acctcgtcgc cgatctgcgg cccctcgtcg
     7681 aagcccacga gctcgtgcat caggacggtg tcggcgccct ccaaccgcac gagaccgatc
     7741 gtccgcggct cgtcgagcgg ctcgccctcc acgcccgcgt acagcacggt gaacgactcg
     7801 atcctccccc gcgggcccac cgtccggtcg gcctcgagct cccccatgca ccgctcgcag
     7861 aagatccggt tcgggcagta gacgatcccg cagcgctcgc atcccgaggc caggaactcg
     7921 cccttcatca gcgaggcgaa gaacgcctcc cccgccaccc ccggcgtgta tcgatgctgg
     7981 agcgggatgt ggccctcgac cagccggacc tccctggggt cgctgatctt ctcgaacatc
     8041 agcccgccct cctcttcgcg ccggaccgct tccgggaccc ggagcgcttg gacgtcctct
     8101 tcgccgccgg cttcctcttg gcccccttcc gaagcgcggc gtcgcgcggc cggaagtagc
     8161 ggatgtcgag gatcgagccc tcgcgctcct cctcgggctt ccacacggcc tcgaccgcca
     8221 tgcccacctc gatccgatcc gggtccacct cgcccagctt gtgcatgaag cccatcatcg
     8281 gcgtgtcgac gtcgagctcc aggacgaacg gcagctcggg ctcctcgagc tcgaccatgt
     8341 ccc
//
LOCUS       DASUTW010000195         3108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1875204, whole genome shotgun sequence.
ACCESSION   DASUTW010000195 DASUTW010000000
VERSION     DASUTW010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875204"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="VF058_06765"
     CDS             complement(<1..1167)
                     /locus_tag="VF058_06765"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX6208049.1"
                     /translation="MGLTIHLLGSPRVERDGVAPEPLRGHKAWGLLAFLVRSQVPPSR
                     ERVAGLLFPEADDPLGTLRWTLSVLRRHLGEQAELGGDPLELKLLPGTLVDVDVLEKG
                     SWIEAVALPGLGHELLDGLTFRASPGFELWLENERRHVAGITSAVLHQAALALLARGE
                     ADAAARHASELVRLDPYDENAHVLLVRCLVASGDPAAASRHVETATELLRRELGIEPT
                     PALRAAAVATEVPVAGRVSRSVDVRAQVEVGGAALAAGGVEAGVHQLRAAAAAARGLD
                     DPALLSEVLVSLGGALGPFRSRHRRGGRRRAPPRDHHRGADGKPRRRRDGVAQISWVQ
                     FLRAHYERAEESLDRAADHAAGDERELAWVDLIRGACRHDVGDYPAAGKLLRSAV"
     gene            1252..2229
                     /locus_tag="VF058_06770"
     CDS             1252..2229
                     /locus_tag="VF058_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208050.1"
                     /translation="MRTKHDERGRRAGRRGLRWAGLAIALAIVAAPGLAGAVEFSDWT
                     LAQKIDEINGNHPDLNTMSLDGCPIQSPNGLRLYMASNRPGSQALDIWVAKRASTDAP
                     FGEPTNLGAPVNSASDDFCPTPVRGKGLYFVSRRAIPGACGLGDIYFTRFNPRHGWRE
                     PVHLGCAPSGPNSALDEQGPSYVEVDGGAQLYFSRSSGAVPGDIFVSTRIGDGAFGPA
                     VAVAELNDATANDIQPNVRKDGREVVFSSNRSGSVENSQDIWVSTRESVSDPWGTPVN
                     LGGNVNTAASESRPSLSWDARQLLFGRAPGPEGMSDIYLSTRERITGSG"
     gene            complement(2356..2655)
                     /locus_tag="VF058_06775"
     CDS             complement(2356..2655)
                     /locus_tag="VF058_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208051.1"
                     /translation="MHARVTTTKGPPDSVDQATQVVTEQVLPSARQLAGYKGILSLAD
                     RTTGTGLTITLWESEEAMAASDEAADAMRGQAVGAIEGSEVASVDRYEVTIDERA"
     gene            complement(2731..>3108)
                     /locus_tag="VF058_06780"
     CDS             complement(2731..>3108)
                     /locus_tag="VF058_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208052.1"
                     /translation="FEEASGLEKARPDVRIKLSRTADYIQLLENVQIHGYHLMMGLGR
                     ALDPAEVAEDWYERVYAPVVQGLRREGIALPDLTEGDLFLELYRRRRDRFAEQGCPSL
                     SETIPELTPEPARPWWAPWRRRP"
BASE COUNT          455 a         1100 c         1119 g          434 t
ORIGIN      
        1 cacggccgag cgaaggagct tgccggccgc cgggtagtcg ccgacgtcgt gccggcacgc
       61 gccgcggatc aggtccaccc acgccagctc gcgctcgtcg cccgccgcat ggtcggccgc
      121 tcgatccagg gactcctccg ctcgctcgta gtgcgcacgg aggaactgga cccagctgat
      181 ctgcgccacc ccatcgcggc gacgccgtgg cttcccgtct gctccgcgat ggtggtccct
      241 tggtggagcg cggcgacgcc ctcctcgtcg gtgccgcgag cggaatggac cgagggcccc
      301 gcccaacgag accagcacct ccgaaaggag cgcggggtcg tcgagccctc gggcggcggc
      361 ggcggcggcc ctgagctggt gcactcccgc ctcgacgccg ccggcggcga gcgccgctcc
      421 ccccacctcc acctgcgccc ggacgtccac gctccgcgag acgcggcccg cgaccggcac
      481 ctcggtggcg acggccgccg cacggagcgc cggggtcggc tcgatcccga gctcgcgccg
      541 gaggagctcg gtcgccgtct ccacgtggcg cgaggcggcg gcgggatcgc ccgatgcgac
      601 cagacagcga accagcagga cgtgggcgtt ctcgtcgtag gggtcgagcc gcacgagctc
      661 ggaggcgtgg cgggcggcgg catcggcctc accgcgagcg agcagcgcga gcgccgcctg
      721 atggaggacc gcgctcgtga tccccgcgac gtgacggcgc tcgttctcca gccacagctc
      781 gaagcccggg ctggcgcgga acgtcagccc gtccagcagc tcgtgaccga ggcccgggag
      841 cgcgacggcc tcgatccagg agcccttctc gaggacgtcg acgtcgacga gcgtgcccgg
      901 cagcagcttc agctcgagag gatctccgcc gagctccgcc tgctcaccga ggtggcggcg
      961 caggaccgac agggtccagc gaagcgttcc cagtggatcg tcggcctccg ggaacagcag
     1021 gcccgccacc cgctcccgcg acggcggaac ctgactgcga acgaggaacg cgagcaggcc
     1081 ccacgccttg tggccgcgca gaggctccgg cgcgacgccg tcccgctcga ccctggggga
     1141 tcccaggaga tggatcgtca aacccacttt acgctctccc cgtcacacgt tgcgtcacac
     1201 gcccgactgt gactctgtcg ctcgattagt tcaagaggag aggagggcag gatgcgcaca
     1261 aagcatgacg aaagggggcg gcgagcgggc cgacgggggc tccgatgggc cgggctggcg
     1321 atcgcgctgg cgatcgtggc ggcgccgggc ctggcggggg cggtggagtt ctccgactgg
     1381 accctcgccc agaagatcga cgagatcaac gggaaccacc cggacctgaa cacgatgtct
     1441 ctcgacgggt gcccgatcca gtccccgaac gggctgaggc tctacatggc ctcgaaccgt
     1501 ccgggcagcc aggcgttgga catctgggtc gccaagcggg ccagcaccga tgccccgttc
     1561 ggggagccca cgaacctcgg ggcccccgtc aactcggctt cggacgactt ctgcccgacg
     1621 ccggtgcgcg ggaagggcct ctacttcgtg agccggagag cgatcccggg agcgtgcggg
     1681 ctgggcgaca tctacttcac ccgcttcaac ccgaggcacg ggtggagaga gccggtccat
     1741 ctggggtgcg cgccgagcgg gccgaacagc gccctggacg agcaggggcc ctcgtacgtg
     1801 gaggtggacg gaggagccca gctgtacttc tcgcggagct cgggggccgt gcccggggac
     1861 atcttcgtga gcacgaggat cggggacggt gccttcgggc cggcggtggc ggtggccgag
     1921 ctgaacgacg ccaccgcgaa cgacatccag ccgaacgtgc gcaaggatgg gcgtgaggtc
     1981 gtcttctcgt cgaaccgctc ggggtcggtg gagaacagcc aggacatctg ggtctcgacg
     2041 cgggaaagcg tgagcgatcc gtggggcacg ccggtcaacc tgggcggcaa cgtgaacacg
     2101 gccgcatcgg agtcccggcc ctcactgtcc tgggatgctc gccagctgct gttcgggcgc
     2161 gcgcccgggc ctgagggcat gtccgacatc tacctctcca ctcgggagag gatcacggga
     2221 tctggctgag cgcggcgtag cgtgcggagg gcgggcccct ttcggggccc gccctcacgc
     2281 ttcgttcgct gaggactccg gcgccgttcg agtccagcag cgcccgggcg gcgcgggtgt
     2341 ggccgcccca gcccttcagg cgcgctcgtc gatcgtgacc tcgtagcggt cgacggaggc
     2401 gacctcggac ccctcgatgg cgcccacagc ctgtccccgc atggcgtcgg ccgcctcgtc
     2461 gctcgccgcc atggcctcct cgctctccca gagggtgatg gtcagcccgg ttccggtggt
     2521 ccggtcggcg agggagagga tgcccttgta gccggccaac tgtcgagcgc tggggagtac
     2581 ctgctccgtc accacctgtg tggcctgatc cacggagtcg ggcggaccct tggtcgtcgt
     2641 gacccgtgca tgcatgacgt ctcctctccg tcgcgatcct ccgggccgag cctaaacccg
     2701 gcgcgcctcg ggaggcaaag gaaacaccgg tcatggtcgt cgccgccagg gggcccacca
     2761 gggcctggcc ggctcgggcg tcagctccgg gatggtctcc gacagggagg ggcatccctg
     2821 ctcggcgaag cggtcgcgcc gccgccgata gagctccagg aacaggtctc cctcggtcag
     2881 atccggaagg gcgatcccct cgcgtctcag cccctgcacc accggcgcgt agacgcgctc
     2941 gtaccagtcc tcggcgacct cggcgggatc gagggcccgg ccgaggccca tcatgaggtg
     3001 gtacccgtgg atctggacgt tctccaggag ctggatgtag tcggcggtgc gcgagagctt
     3061 gatccgaacg tccgggcggg ccttctccag gccgctcgcc tcctcgaa
//
LOCUS       DASUTW010000196         5738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_320379, whole genome shotgun sequence.
ACCESSION   DASUTW010000196 DASUTW010000000
VERSION     DASUTW010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320379"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..503)
                     /locus_tag="VF058_06785"
     CDS             complement(225..503)
                     /locus_tag="VF058_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208053.1"
                     /translation="MKTTDPLSPAAREIERAWTQRSILRDRLDERRERVADLWVRALV
                     SETPQADQAYRLAEEECRRVEAEIARIEERLGAFGTPSVGPLGSGGSP"
     gene            complement(562..635)
                     /locus_tag="VF058_06790"
     tRNA            complement(562..635)
                     /locus_tag="VF058_06790"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(599..601),aa:Leu,seq:caa)
     gene            701..1687
                     /locus_tag="VF058_06795"
     CDS             701..1687
                     /locus_tag="VF058_06795"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEX6208054.1"
                     /translation="MRVAVCTDMEGISGVDRYEQCFPGWTADYRHGVRMLVAEVGAAV
                     EAALDAGATEVVLADWHYLGRNLPAAAFPDLPIRRLWRSGRPTASVEDLGRPDAAILL
                     GIHAGAGNPEGFLSHTLWMGASVLIEGVPVSEAVLWAWALGAGGVSVAAIAGDSRAIE
                     EAGDLLPGLPAVGVKAGTSRASAILRPPQDAREEIAETVRRGLGDVPPPLDRPFPADV
                     TIRFARGDDAAKVAGAGVAERTGPRDVSSRLGSGHDLVPFLGRALLATPLGTRLSLTD
                     RLLPRGGGSWAALRHAGLDPVVGWAERRLVRRWADEPAHLYPRVGARPSPAG"
     gene            complement(1688..2272)
                     /locus_tag="VF058_06800"
     CDS             complement(1688..2272)
                     /locus_tag="VF058_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208055.1"
                     /translation="MRRSLAWILTVLGGLVMGVGPLLPFVVADTPQGTLSYSGIAQEI
                     GGDGWYFIGVGAALVVLGASIAWSRSATLARVLGAIGVLAAGLVAAVAAIDIVDIITL
                     EEELPPEAGGLFSFSVGIGLYVAAAGSIVGLIGSGTALFVQPEAPPVQPLAGPPTGWS
                     PEEEPPRPPLWPPPPDSRPSGSPPPQSPPAPPES"
     gene            complement(2275..4878)
                     /gene="dnaE"
                     /locus_tag="VF058_06805"
                     /pseudo
     CDS             complement(2275..4878)
                     /gene="dnaE"
                     /locus_tag="VF058_06805"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
     gene            5084..>5738
                     /locus_tag="VF058_06810"
     CDS             5084..>5738
                     /locus_tag="VF058_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208056.1"
                     /translation="MLLDAEEDVVGLVVLLVRVVEVVRGHERDAEAARDLDEGGRDLR
                     LVGQPVVLELDVERALPEDVAVGGGGLLGLLPLIGQEVPAHLGGEAAREPDQPLGVAG
                     EQLLVDPRTVVEALEVGVGDEPQEVPVAGLVPGQDREVPVLLLVLAGVAVEPRARRHV
                     GLDAEDRLDPSRPGLTVEVERAEHHAVVGEADRRHPHLGGPVEHRGRAAARRDAREPV
                     "
BASE COUNT          783 a         2093 c         2018 g          844 t
ORIGIN      
        1 tcactccgtg ggtcacggca cggagatgct cggcttcctc cctcctttct cggacccgga
       61 tgccggctcg cgcgtctccc gagcaagccg ggcatgaatt accacatcgc cgcgccgatt
      121 gccagattcc cgccggccgg ccgccgtgtc ggtcgttcgg ccgaggcccg gcgcgtgcga
      181 cccctcgata tgggtacgaa tcgccgggcg cgcgggattg accatcacgg cgagcctccc
      241 gatccgaggg gccccaccga cggggtcccg aaggctccca ggcgctcctc gatgcgggcg
      301 atctccgcct cgacccggcg gcactcctcc tccgccaggc ggtacgcctg gtccgcctgc
      361 ggcgtctccg ataccagggc cctgacccag agatcggcga cccgttcgcg gcgctcatcg
      421 agccgatccc gcaggatcga gcgctgggtc caggcgcgct cgatctcgcg ggcggccggg
      481 gagagggggt ctgtggtctt catcgaggtt cgcgaaagtc tatggaaccg agaaccccgt
      541 cgcaagctat ggagggaggc gtgccggggg tgggattcga acccacactg cgccgggttt
      601 gagccggcgg cctctgccga ttgggctacc ccggccgcgc tccatagtac ctcccgggag
      661 cgcttggccg acgcacccgg ggcgtgggac gatggcgctc atgcgggtgg cggtgtgcac
      721 cgacatggag ggcatctccg gcgtcgatcg ctacgagcag tgcttcccgg gctggaccgc
      781 cgactaccgg cacggcgtcc ggatgctcgt ggccgaggtg ggggccgccg tcgaggcggc
      841 gctcgacgcc ggggccaccg aggtcgtgct cgcggactgg cactacctgg ggaggaacct
      901 cccggccgcg gccttccccg acctccccat ccggaggctt tggcgatccg gccggcccac
      961 cgcgagcgtc gaggacctgg gccgccccga cgccgcgatc ctcctgggga tccacgcggg
     1021 cgccgggaac cccgaggggt tcctcagcca caccctctgg atgggggcct ccgtgctgat
     1081 cgagggcgtg ccggtctccg aggcggtcct ctgggcctgg gccctgggtg ccggcggcgt
     1141 ctccgtggcc gcgatcgcgg gcgactcgcg ggcgatcgaa gaggcggggg acctcctccc
     1201 gggcctcccg gccgtcggcg tgaaggcggg gacgtcgcgc gcctcggcca tcctccgacc
     1261 gccccaggat gcgagggagg agatcgcaga gaccgtccgg cgcgggctgg gcgacgtgcc
     1321 cccgcccctg gaccgcccct tccccgccga tgtcacgatc cgcttcgcgc gaggggacga
     1381 cgcggcgaag gtggccgggg ccggcgtggc cgagcggacg ggcccccgcg acgtctcgtc
     1441 ccgcctcggc tccggccacg acctcgtccc cttcctgggc cgggccctcc tggccacccc
     1501 cctggggacc cggctctccc ttaccgaccg cttgcttccc cgcgggggcg gctcctgggc
     1561 cgccctccgc cacgcagggc tggacccggt ggtgggatgg gcggagcgcc ggctcgtccg
     1621 ccggtgggcg gacgagccgg cgcacctgta cccgcgggtg ggggcgcgcc ccagcccggc
     1681 gggctagcta gctctcggga ggcgccgggg ggctctgcgg cggcggagag ccggacggac
     1741 gagagtcggg cggcggaggc cagagcggag gccgaggagg ctcctcctca ggcgaccatc
     1801 cggtcggggg ccccgcaagg ggctggacgg gcggggcctc cggctgcacg aagagggcgg
     1861 ttccgctccc gatcagcccg acgatcgagc ccgcggcggc gacgtagagg ccgatgccga
     1921 ccgagaagct gaacagcccc cccgcctccg gaggcagctc ctcctccagg gtgatgatgt
     1981 cgacgatgtc gatcgccgcc accgcggcca ccaaccctgc ggccaggacg ccgatcgcgc
     2041 cgaggacgcg tgccagggtc gccgaccggc tccatgcgat cgaggcgccg aggacgacca
     2101 gcgccgcccc caccccgatg aagtaccagc cgtcgccacc gatctcctgg gcgatgcccg
     2161 agtagctcag cgtgccctgg ggagtgtccg cgacgacgaa ggggagcagc ggcccgaccc
     2221 ccatcacgag gccccccagc acggtcagga tccaggcgag cgagcgcctc acggtcaacg
     2281 gccggcgggg gccgcgcgcc ccacggcctc cgcccccagg aggctcctga gctccgagag
     2341 gaggccggcg gaaccgtcca cgcggtagcc gtcggccagc cgcagcagct tcgcgctccc
     2401 gttcgaccgg aggcggagat ggacggcgac gcgtccgggg tgtccggaga gcacctcctt
     2461 cagcgccgtc gccaggccgt cggtgcactg ctcggccggc acctccagga ggatcggctc
     2521 ggcgggcggc ggcggctcgc ccgagtcgtc gagcgccccg atctccacgg ccacgagctg
     2581 cagctcccgt ccccgcaggt ccacccggcc cttgacgagc acgatccggt ccggggccag
     2641 caggtcggtg accttctcga acaccgaggg gaacgccacc acctggacgc cgcccacgag
     2701 gtcctcgagc cgcaggatcg cgtagggctc ccccttcttc gtgaaccggc ggttcacgga
     2761 cccgatgatc cctccgacgg tgaccacgtc gccgtccccg aggcccacga gcgccgaggt
     2821 gtccacgtcg gtctgggcct ccaggcgctc gcgcaccgcg aggagggggt ggtcggtgac
     2881 gtactgcccc agcacctgct tctccagcgc caacagctcc tgcttctcga actcgggccc
     2941 ctcgagcacc gcctcgtcga tctcctgaga ggcctgggct ccctgcccga acagcgagaa
     3001 ctgccccgca gcctcggccc gccgctggga ggcgatcggt cccgccaccc ggtcatagcc
     3061 ctcgagcagg ccccgccgcg tgtgtccgag cgagtcgaac gcgcccgaaa ggatcaggct
     3121 ctccagcacc ttcttgtgca gcaccgacgg atccaccttc cggcagaagt ccgcgaacga
     3181 ggtgaactcg cccttctccc gacgggccgc gatgatctgc tccacggcgc ccgcccccac
     3241 gttccgcacc gccgagagcc cgaacctcac ctcgccgccc accggagtga agtcgacggc
     3301 ggaggcgttc acgtccggcg gcaggacgcg gatgtcggcc aggcggcagg cgttcaggta
     3361 gaagggcttc ttgtccttgt cgtccttgac gctcgtcagc agggcggcca tgtattcgac
     3421 gggatggtgt gcccggaggt acgcggtctg gtaggcgacg agggcgtacc cgcaggcgtg
     3481 cgaggcgttg aacccgtagt cggcgaacgg catgatcaag tcgaagatct cgttggccag
     3541 cgcccgctcg tacccccgct ccacggcgcc ggccacgaac ttctcccggt gcacgaccag
     3601 ctgctcgcgg atcttcttcc ccatcgcctt gcggaggagg tcggcctcgc ccatcgagta
     3661 gccggccatc cgcacggcga tctccatgac ctgctcctga tagacgatga ccccgtaggt
     3721 gtccttgagg atcggctcca ggtcggggtg cgggtacacg acgggcttgc ggccgtgctt
     3781 ccgctccgcg tactccacgt gcatcccggc gttgagcggc cccgggcggt agagggagac
     3841 cagcgccatc aggtcctcga agcggttcgg caggagctgg cggatcagcg agcgcatccc
     3901 ctcgccctcg agctggaaga cgcccgtcgt ctcgccgcgc cccagcatcg cgtacgtctc
     3961 ctcgtcgtcc agcggcaggt ggtcgacgtc gagctcgaac ccctggtccc gcagcatccg
     4021 caccgtgtcg tcgatcaccg agaggttccg caggccgagg aagtccatct tgaggaggcc
     4081 cagtttctcc acgccgtgca tgtcgaactg cgtgacgacc cgcttcgagt cgtcgcggga
     4141 gtccttcgac agcttcaggg ggaggtagtt cacgagcggc cggtcgccga tcacgactcc
     4201 ggcggcgtgg accgagtcct cgcggcggag cccctcgagc gcgcgcgccg tgtcgacgat
     4261 cgtgcgggcg tcgtcctccc gattgtaggc gtccttcagc tcggccgaga tctccagggc
     4321 ctgctggatc gggtactcgc ggcccagcac cgccgcgggg aacatcttgc acagccggtc
     4381 ccccatcgcc gccgggaagc ccagcacccg ggccgcgtcg cggatcccct gcttcccctt
     4441 gatcgtctgg aacgtgatga tctgggccac gtggtcgtga ccgtaggtgt ccgcgacgta
     4501 gcggatcacc tcgtcgcgcc ggcgctcgtc gaagtccatg tcgatgtcgg gcatctgctt
     4561 ccgctcgggg ttgaggaagc gctcgaagat cagcttgtag cggaggggat cgaggtcggt
     4621 gatgcgcagg cagtacgaga ccaccgagcc cgcggccgat ccccgacccg gccccacgcg
     4681 gatcccgcgc tctcgcgcga agcggatcag gtcccagacg atgaggaagt agcccgcgaa
     4741 ccccatctcg ttgatgatcg tcaactcgtg ctccagcctg gcccggagct cgggcccgat
     4801 ctcgccgtag cgctgctcgg cgccctcgag ggtcagctcg cggaggtacc ctttctggtc
     4861 cttccccgcg ggggggctcc agccggggca gccggtggac cacctcgccg tccaccagga
     4921 cgccctccag gtcggggatc ggctccaccg actcggcgat ctcgagggtg gcgtcgcagg
     4981 cctcgggcag ctcgcggaag acctcgcgca tctcctgggc cgacttcagg tagaactcct
     5041 cggtctcgaa ccgcagacgg ttcgtgtcgg tcaggacctt cccgtgctgc tggatgcaga
     5101 ggaggacgtc gtgggcctgg tggtcctcct tgtccgtgta gtggaggtcg ttcgtggcca
     5161 cgagcgggac gccgaggcgg cgcgcgatct ggatgagggt gggcgtgacc ttcgcctggt
     5221 cggccagccc gtggtcctgg agctcgacgt agaacgagcc ctccccgaag atgtcgcggt
     5281 aggcggcggc ggcctcctcg gccttcttcc cctgatcggc caggaggtgc cggcacacct
     5341 cggaggcgag gcagcccgag agcccgatca gcccctcggc gtggcgggcg agcagctcct
     5401 tgtcgatcct cggacggtgg tagaagccct cgaggtgggc gtcggagacg agccgcagga
     5461 ggttccggta gccggtctcg ttccgggcca ggatcgtgag gtgccggtac ttctcctcgt
     5521 cctcgccggg gttgcggtcg aaccgcgagc ccggcgccac gtaggcctcg acgccgagga
     5581 tcggcttgat ccctctcgcc cgggcctcac ggtagaagtc gagcgcgccg aacatcacgc
     5641 cgtggtcggt gaggccgatc gccggcatcc ccatctcggc ggcccggtcg agcatcgagg
     5701 gcgcgctgct gcccggcgtg atgcgcgcga acccgtcc
//
LOCUS       DASUTW010000197         2595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_968583, whole genome shotgun sequence.
ACCESSION   DASUTW010000197 DASUTW010000000
VERSION     DASUTW010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968583"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="VF058_06815"
     CDS             <1..1135
                     /locus_tag="VF058_06815"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX6208057.1"
                     /translation="RGVRVPGTLPGRGVHMRPSSRSTLRVLAATAVAVLALASGPVAQ
                     ADPPEQAERRVDVMTYNLYFGADLSPLFDPEVDTLPELIVAAASVFAQTQQTDFPSRA
                     EVIAREIAEQSPSVIGLQEVAIIQTAAITDPGTPLSTLDFLDILLAELEERGAHYDAV
                     ATNVNFSGTLPISPTTLGTLTDRDVILARTDLPTSQLKLSNPTLHTFEATLTLSAGGV
                     PLEIIRGWSSVDVKLRGKPFRFVNTHLEAFHPVIRQVQAAELIEWMDDSPLPVLLAGD
                     LNTLRGDPADAYGLFVDAGFVDAWVDAMPGDPGFTAGQAADLMGPSLLDHTVDYVMYE
                     GFLDAVSGSGEIIGEEDPADRTPSGLWPSDHAGVNVTIHVARP"
     gene            1213..2493
                     /locus_tag="VF058_06820"
     CDS             1213..2493
                     /locus_tag="VF058_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208058.1"
                     /translation="MGVRKIAVSITLTLALLTIGAPGRASVPPVNDDFASAIDVAALP
                     FENIVDTTEATLEQDEPQPPCTFFTGNTVWYEFTPGSDTLVSVDTLGSGFDSVLAVWT
                     GAELSSLDLVACNDDALSLNAQTSFRARSATTYHIQVGGFDSAAGSLLLRIRTVEAGV
                     IEGTVTREGTGAPLPGICVEALDRDLFSAQTAVTGADGAFELVVRGGSYLVGFIDFCD
                     PSDDHIPEWFDDVAFSEPQNATPIDLSPGDVVSGKDAELAGGCPGIASLAAVLGFNQV
                     VGTAGPDDLVGSGDEDVLCGLGGHDTLMGLAGLDVLFGGPGRDRLLGGQGSDELIGAL
                     GKDALIGGPGPDLLLGGRGNDRLRGGTGNDLLSGGPGRDTCAGGPGADLARGCEVSRS
                     VIRSRSRVIVRGSTGAISAREPRSLDELVAILRR"
BASE COUNT          365 a          906 c          927 g          397 t
ORIGIN      
        1 ccgcggcgtt agagtgccgg gcacgcttcc gggcaggggg gttcacatgc gaccgtcgtc
       61 acgctcaacg ctccgcgtcc tcgccgccac cgccgtcgcg gtcctcgcgc tcgcgtcggg
      121 cccggtggcc caggccgacc ccccggaaca ggctgagcga cgcgtcgacg tcatgaccta
      181 caacctctac ttcggggccg atctctctcc gctcttcgat cccgaggtgg acaccctgcc
      241 cgagttgatc gtggcggccg ccagcgtctt cgcccagacc cagcagaccg acttcccgtc
      301 gcgcgccgag gtcatcgcga gggagatcgc cgagcagtcg ccgtcggtga tcgggctcca
      361 agaggtggcc atcatccaga cggcggccat caccgacccc ggcaccccgc tcagcacgtt
      421 ggacttcctc gacatcctgc tcgcggagct cgaggagcgc ggggcccact acgatgcggt
      481 cgccaccaac gtgaacttca gcgggaccct gccgatcagc cccaccacgc tcggcaccct
      541 gaccgatcgc gacgtcatcc tggccaggac cgatctcccc acctcgcagc tcaaactgtc
      601 caacccgacg ttacacacct tcgaggccac gctgacgctc tcggcggggg gcgtgccact
      661 cgagatcatc cgcggctggt cgagcgtgga cgtgaagctc cgcgggaagc cgttccgctt
      721 cgtgaacacg cacctcgagg cgttccatcc ggtgatccgg caggtccagg cggccgagct
      781 gatcgagtgg atggacgatt cgcccctgcc cgtcctcctg gccggtgacc tcaacacgct
      841 gcggggggac ccggcggatg cctacggtct tttcgtggac gcgggcttcg tggacgcctg
      901 ggtggacgcg atgccggggg atccggggtt caccgcgggg caggcggccg acctgatggg
      961 gccctcgctc ctcgaccaca cggtggacta cgtgatgtac gagggcttcc tcgacgcggt
     1021 gagcgggagc ggggagatca tcggcgagga ggatcccgcc gaccgcaccc cgtcggggct
     1081 gtggccctcg gaccacgccg gcgtgaacgt cacgatccac gtggcgagac cctgagacgg
     1141 aacagtcgga cactccttga cggtcccccc gagagggtgg atgctgtgcc cccatctcga
     1201 agggggccgc acatgggggt tcgcaagatc gccgtctcga tcacgctcac gctcgccctg
     1261 ctgacgatcg gagcgccggg gagggcgtcc gtgccgccgg tcaacgacga cttcgcctcg
     1321 gccatcgacg tcgccgccct gcccttcgag aacatcgtgg acacgaccga ggccacgctc
     1381 gagcaggacg agccccagcc tccgtgcacg ttcttcacgg ggaacacggt ctggtacgag
     1441 ttcacgcccg ggtccgacac cctcgtgtcc gtcgacacgc tggggagcgg cttcgactcg
     1501 gtgctggccg tgtggacggg ggccgagctg agctcactgg atctggtggc gtgcaacgac
     1561 gatgctctgt ccctcaacgc ccagacctcg ttccgagccc ggtccgccac gacctaccac
     1621 atccaggtgg gtgggttcga cagcgcggcg gggtccctcc tcctccggat ccggacggtg
     1681 gaggcggggg tgatcgaggg gacggtcacg cgggagggga cgggggctcc gctcccgggg
     1741 atctgcgtgg aggcgctcga cagggatctc ttctcggccc agacggcggt caccggggcc
     1801 gacggcgcct tcgagctcgt cgtccgcggg ggctcgtacc tggtcgggtt catcgacttc
     1861 tgcgatccgt cggacgatca catccccgag tggttcgacg atgtggcctt ctccgagccc
     1921 cagaacgcca cgcccatcga cctgtccccg ggggacgtgg tgagcggcaa ggacgccgag
     1981 ctggccggag gatgtcccgg gattgcctcc ctggcggcgg tgctgggctt caaccaggtc
     2041 gtggggaccg ccggtccgga cgacctggtg gggagcgggg acgaggacgt gctgtgcggg
     2101 ctcgggggcc acgacacgct gatggggctg gcagggttgg acgtcctgtt cggcggcccg
     2161 ggcagggacc gccttctggg tggccaggga agcgacgagc tgatcggcgc cttgggcaag
     2221 gacgcgctca tcggcggtcc cggaccggac ctcctcttag gagggcgggg caacgaccgg
     2281 ctccgcgggg ggaccggcaa cgacctcctc tcgggcgggc ccggccgcga cacgtgcgct
     2341 ggaggaccgg gggccgacct cgctcgggga tgcgaggtct cccggagcgt catccgctcg
     2401 aggtctcggg tcatcgtccg cgggtcgacc ggggcgatca gcgcgaggga gcctcgctcg
     2461 ctcgacgagc tcgtcgcgat cctgcgtcgg tgaccgccgg aggggccgcg ctccggcgcg
     2521 gcccctccgg cggtcacccg gtgttggggg cgccgggcgt caccgtcgcg gtgaaggccc
     2581 agtcggtggc cgcat
//
LOCUS       DASUTW010000198         8412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_62211, whole genome shotgun sequence.
ACCESSION   DASUTW010000198 DASUTW010000000
VERSION     DASUTW010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62211"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VF058_06825"
     CDS             complement(<1..560)
                     /locus_tag="VF058_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208059.1"
                     /translation="MTPLDDYRDEMAILGSEQIEEVLAGRPVDGPGAEPLARLARDLR
                     GGLLVDLHPEIEARHLAAMRAAVATAPRRIRPRRVAAKLALAAALVLGAGVAAATTAR
                     LVGSPSIVPEDLPLPVHGESDSRTQVPVSEPAPGSVATADRPSRPEADTIRAPSARSK
                     GCGAAAAAPRAAAACHQGLSKSEGKG"
     gene            complement(557..1105)
                     /locus_tag="VF058_06830"
     CDS             complement(557..1105)
                     /locus_tag="VF058_06830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6208060.1"
                     /translation="MPPFEEMLDAARAGEEWALEAIYCDLSPAVAGYLRGQRAAEPED
                     LTSEVFVGLVRGLRAFCGDERAFRSWVFSIAHRRLLDERRRLARRRERPADPGELAAE
                     GPKGNVEAESLARLGDGWAAELLDMLTDEQRTVVLLRIVADLSVREVAAVTGRSPGAV
                     KTMQRRALLQLARRVEREGVSP"
     gene            complement(1149..2549)
                     /gene="pyk"
                     /locus_tag="VF058_06835"
     CDS             complement(1149..2549)
                     /gene="pyk"
                     /locus_tag="VF058_06835"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX6208061.1"
                     /translation="MTRATKLVCTLGPASAGRVLELVEAGMDVARINLTHGTREDHEE
                     LLRSVREASREAGRAVAVMTDLSGPKVRLGELEPEEVRLEDGNAFVLRGGEGPGDARG
                     APTSHAGLAEDLEVGDRVLLSDGVVELRVEDTTGDDVRTRIVRGGLIRSRAGVNVPSG
                     RLSVPAITETDERDLAWVRSARIDLVAQSFVRHAAEVRALADLLRGEPALLVAKIETR
                     SAVEGADGILEAADAVMVARGDLGVELPRERIPVIQKDLVDRANRSGVPAVVATQMLE
                     SMTGSRRPTRAEASDVAGAAFDGAAAILLSAETAIGRYPVEAARTAAAILETAEREGA
                     RFISTTVEERVEGSDAWAVADAAATAAADSGAVAVACFTRTGLTARLLSGVRLPVPVY
                     AYSHDDAVVRRLTMFRGVHPRPCEPPEDTDAMLAMMDRRLREEGLASSGDPVVLVAST
                     PVGRSRTNLLKVHRVG"
     gene            complement(2546..3676)
                     /locus_tag="VF058_06840"
     CDS             complement(2546..3676)
                     /locus_tag="VF058_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HEX6208062.1"
                     /translation="MSDRVALVYSSEVVEYDHGPQHPLKPVRVLLTRELIAAYGVLEA
                     GHVDEVPTRVASDEEVGLVHTQAYIDVVKAAGSGERGDWWAYGFGPGDNPIFPAMHEA
                     SARVVGASIEAARLVLTGEADHAFNPAGGLHHAMPERASGFCVYDDPAVAIRWLLDRG
                     VERIAYVDVDVHHGDGPQAIFYDDPRVLTISLHESGRYLFPGTGFVSERGEGEAEGTK
                     VNVPLPPWTSDEAWLQAFREVVPPLVRRFHPDVLVTQLGCDTHHTDPLAHLALSTRAY
                     REASVELHDLAHGVAGGRWVATGGGGYQWARVVPRAWAIYFAEMAGVELSDDLPEGWR
                     QLAAEHARGPVPERLSEEPPEVPAGSGEVRSVVDDVKRAFSV"
     gene            complement(3693..5177)
                     /locus_tag="VF058_06845"
     CDS             complement(3693..5177)
                     /locus_tag="VF058_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015156064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6208063.1"
                     /translation="MIESYQNWIGGKWVPSASGDTFESRNPANMDEVVGTFPRSSADD
                     VDAAVRAAKDAYDDWMRTPAPERADYLLRVALLLEERIEEWSELMTREMGKTLKESRG
                     DIQEGIDFLHYMAGEGRRMQGHTIPSELRDKFSMTVRHPIGVVGLITPWNFPIAIPTW
                     KLAPAIVSGCTSIFKPAEDTPLLATLLVQAFEEVGLPPGVLNLVHGFGEEAGAPMVEH
                     PDIRAISFTGSLEVGRKINERCGALMKRCSLELGSKNVMIVMPDAEMDLAVEAASWGA
                     FATSGQRCTATSRLIVHDAAREEFTERMLKRVEGMKVGSGLDPEVELAPVINATQKER
                     ILEYIEIGKQEGATLLTGGEELTGDGYEKGNFIAPTVFDDMTPDMRIAQEEIFGPVTG
                     IIRTDDVDEAIRMANSVDYGLSAAIYTHDITNVFKAVTQLEFGIVYVNAPTIGAEIQL
                     PFGGMKNTGNGHREAGPQALDEFMEWKAVSVDYSGKVQKAQGID"
     gene            5245..6129
                     /locus_tag="VF058_06850"
     CDS             5245..6129
                     /locus_tag="VF058_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX6208064.1"
                     /translation="MRLVTYAREGKRRLGVLVDETVVDLPDAVGHPAFPETMEAMVRT
                     GPGGLGVALEALERDGTVERHAVLGARLLAPLVPGSLRDFLAFEDHVREGATRRGQPF
                     PEPWHRMPVYYKGNHRSIYGPDEEVPWPRYTSELDYELEIACILGGRGRDLDEEGAAA
                     LIFGYTLMNDWSARDVQRDEMACGLGPAKAKDFATSLGPCVVTADELDPTDLWLTARV
                     DGETWSKGNLGASRWTFPQMIAHVSQDEEVWPGDVYGSGTFGGGCGLDLDRSLRPGAV
                     VELEADRIGILRNPVAAA"
     gene            complement(6126..7070)
                     /locus_tag="VF058_06855"
     CDS             complement(6126..7070)
                     /locus_tag="VF058_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6208065.1"
                     /translation="MRMRPLGASEISVIGFGAWEAGGGVWGENESDDRVIEAIRTGLD
                     AGITWIDTAEGYGSGRSEELVGKAIEGREAFVATKVAPKPEGTGHRPEEVRRACLASL
                     ERLGIGAIDLYQLHWPSDDVPVEETWGAMAELADEGLVRELGLSNFGREQIERCEAIR
                     TVDSLQPQFSILHLKHRDLIRWCGERGIGVVTYGPLAYGLLTGAIDEDTEFADDDWRS
                     GRGAGMSYYDALFAPGKLERSLAVVDRLRPIAERLGITPAQLSLAWILHQPGVTAAIA
                     GSRNPDHVRQNAEAGDVELSPDVLDEIEGILPLGPAFA"
     gene            complement(7091..7966)
                     /locus_tag="VF058_06860"
     CDS             complement(7091..7966)
                     /locus_tag="VF058_06860"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEX6208066.1"
                     /translation="MNGRPLTPATVLLALAIAATLAMGTVRALTFEPVPITGAPTPEG
                     ALRAYYEALEEEDCRAAARYVDPAFLTAEQLCERFEETVAASGSLVGVTDREVGEETA
                     TLIAERVLGDHTDHRVVRVARAGDLWRLAGGSSCFPVLHPTDLGTAHLEEDQAFDDYS
                     SIPPTSGPHSPVPTTAGAVYQEPQPLPEVVHAMEHGAVVVWVSPEAPEPMRERLLRTV
                     SSTAEEGYASLIVTPLPGLEEPFAMTAWGSLQRCLGVSPPEIHAFVEAHYASGGEGAL
                     ACAGPAAEVPRCRDA"
     gene            8029..>8412
                     /locus_tag="VF058_06865"
     CDS             8029..>8412
                     /locus_tag="VF058_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX6208067.1"
                     /translation="MGYRIGVLGATGLVGEVLLSILDERGFPVDELRAFASERSAGRR
                     IGWRGEEVVLEAPAERRFAGLDLVFTAAGSEVAREWVPRIRAQGPVVIDNSSAFRQDP
                     GVPLVIPEINAHALRDHDGLIANPNC"
BASE COUNT         1119 a         3182 c         2928 g         1183 t
ORIGIN      
        1 ttgcccttgc cctcgctctt cgagagcccc tggtggcagg cggcggcggc ccgaggggcc
       61 gccgccgccg ccccacagcc cttcgagcgg gcggagggcg ctcggatcgt gtctgcctcc
      121 ggcctcgatg ggcggtcggc cgtcgcgacg ctccctgggg cgggctcgct cacgggcacc
      181 tgggtgcggc tgtcgctctc gccgtggacc gggaggggca gatcctcggg cacgatggag
      241 ggcgacccca cgaggcgggc ggtcgtggcg gcggcgacac cggcgccgag cacgagggcg
      301 gccgccagag ccagcttcgc cgccacccgc cgcggccgga tgcgtcgcgg ggcagtcgcg
      361 accgcggccc gcatcgcggc gaggtgccgc gcctcgatct ctgggtggag gtcgacgaga
      421 agccccccgc ggaggtcgcg ggcgagccgg gcgaggggct cggcgcccgg cccgtcgacc
      481 ggacggcccg ccagcacctc ctcgatctgc tcggagccga ggatcgccat ctcgtcccgg
      541 taatcgtcga gcggcgtcac ggggaaaccc cctcccgctc gaccctgcgc gcgagctgca
      601 ggagggcccg ccgctgcatc gtcttcacgg cccccggcga cctcccggtg accgcggcca
      661 cctccctgac cgagaggtcg gccacgatcc ggaggagcac cacggtccgc tgctcgtcgg
      721 tcagcatgtc gaggagctcg gcagcccagc cgtccccgag cctggccaga ctctcggcct
      781 cgacgttccc cttcggcccc tcggccgcca gctcgcccgg gtcggcgggc cgctcccgcc
      841 gccgagccag gcggcgtcgc tcgtccagga ggcggcggtg ggcgatcgag aagacccagc
      901 tccggaacgc ccgctcgtcg ccgcagaacg cgcggagccc ccgtaccagc cccacgaaga
      961 cctccgaggt caggtcctcg ggctcggccg cccgctggcc gcggagatac ccggcgaccg
     1021 caggcgagag atcacagtag atcgcctcca gggcccactc ctcaccggcc cgggctgcgt
     1081 cgagcatttc ctcgaagggg ggcatgaagc cattatcgac aggggcggcg gccggcgcac
     1141 atcgaggatc agcccacccg gtggaccttc agcaggttgg tccgggacct ccccaccggc
     1201 gtcgaggcga cgaggacgac cggatcgccg ctcgacgcca ggccctcctc ccggaggcgt
     1261 cgatccatca tcgccagcat cgcgtccgtg tcctcgggcg gttcgcacgg tctcgggtgg
     1321 acgccccgga acatcgtgag cctgcggacc accgcgtcgt cgtggctgta ggcgtagacg
     1381 gggacgggca gccggacgcc cgagaggagt cgggccgtca ggccggtgcg cgtgaagcag
     1441 gccacggcga ccgcccccga gtccgcggcc gcggtggcgg ccgcgtcggc caccgcccac
     1501 gcgtcgcttc cctccacgcg ctcctcgacc gtcgtggaga tgaagcgggc tccctcgcgc
     1561 tcggccgtct cgaggatcgc cgcggccgtg cgcgcggcct cgaccgggta gcgtccgatc
     1621 gcggtctcgg ccgagagcag gatggcggcg gccccgtcga aggccgcgcc ggccacgtcg
     1681 ctggcctccg cccgcgtagg acgccggctc cccgtcatcg actcgagcat ctgcgtggct
     1741 acgaccgcgg ggacccccga gcggttggcc cggtccacga gatccttctg aatcacgggg
     1801 atgcgctcgc gcggaagctc caccccgaga tcgccccgcg ccaccatgac cgcgtcggcc
     1861 gcctcgagga tcccgtcggc cccctccacc gccgagcgcg tctcgatctt cgccacgagg
     1921 agcgcaggct caccgcgcag gaggtccgcc agcgcccgca cctcggccgc gtggcggacg
     1981 aaggactggg cgacgaggtc gatcctcgcg ctcctcaccc acgccaggtc ccgctcgtcc
     2041 gtctccgtta tggcggggac gctcagccgt cccgacggga cgttgacccc ggccctggag
     2101 cggatcaggc ctccccgcac gatcctcgtc cggacgtcgt cgccggtcgt gtcctcgacc
     2161 cgcagctcca cgacgccgtc cgagagcagg acacggtcgc ccacctcgag gtcctccgcg
     2221 agacctgcgt gactggtcgg cgccccccgc gcgtcgcccg ggccctcccc gccgcggagc
     2281 acgaacgcgt tcccatcctc cagccggacc tcctccggct cgagctctcc cagcctgacc
     2341 ttggggcccg acaggtcggt catcaccgcg accgcgcggc ccgcctcccg ggaggcctcc
     2401 cgcaccgacc gcaggagctc ctcgtgatcc tcgcgggtcc cgtgcgtcag gttgatgcgg
     2461 gccacgtcca tgcccgcctc cacgagctcg agcacgcggc ccgcgctcgc ggggccgagc
     2521 gtgcagacca gcttcgtggc cctggtcata ccgagaacgc ccgcttcacg tcgtcgacga
     2581 ccgaccggac ctcgcccgag ccggccggca cctcgggcgg ctcctcggag aggcgctccg
     2641 gcacgggccc ccgggcgtgc tccgcggcca gctgcctcca cccctcgggg agatcgtcgg
     2701 agagctcgac cccggccatc tccgcgaagt agatagccca ggcccggggc accacccggg
     2761 cccactggta cccgcctccc ccggtggcga cccagcgccc cccggcgacc ccgtgcgcca
     2821 ggtcgtgcag ctccaccgac gcctcgcggt aggcccgggt cgagagcgcg aggtgcgcca
     2881 gcgggtcggt gtggtgcgtg tcgcagccga gctgggtcac gagcacgtcc ggatggaagc
     2941 gccgcaccag gggcggcacg acctcgcgga aggcctggag ccaggcctcg tcgctcgtcc
     3001 agggcggcag cggcacgttg accttcgtcc cctccgcctc gccctcgccc cgctccgaga
     3061 cgaagcccgt ccccgggaag aggtagcggc cgctctcgtg gagcgagatc gtcagcaccc
     3121 tgggatcgtc gtagaagatg gcctgcggcc cgtcgccgtg gtgcacgtcc acgtccacgt
     3181 acgcgatccg ctccacgccg cggtcgagca gccaccggat ggccaccgcg ggatcgtcgt
     3241 agacgcagaa cccgctggcc cgctctggca tcgcgtggtg gaggccgccc gcggggttga
     3301 acgcgtggtc cgcctctccc gtgagcacca gcctcgcggc ctcgatcgag gcgcccacca
     3361 cccgggccga cgcttcgtgc atcgcgggga agatcgggtt gtcgccgggc ccgaacccgt
     3421 aggcccacca gtccccgcgc tcccccgatc ccgcggcctt gaccacatcg atgtaggcct
     3481 gggtgtgcac gaggccgacc tcctcgtcgg aggccacgcg cgtcgggacc tcgtccacgt
     3541 gacccgcctc cagaaccccg tacgccgcga tcagctcgcg ggtcaggagg acccgcacgg
     3601 gcttgagggg gtgctgtggc ccgtggtcgt actcgacaac ctccgacgag tagacgaggg
     3661 cgacgcggtc gctcacgggc gccggccggt cctcagtcga tcccctgggc cttctggacc
     3721 ttccccgagt agtccaccga gacggccttc cactccatga actcgtcgag ggcctggggc
     3781 cccgcctccc gatggccgtt cccggtgttc ttcatcccgc cgaacggcag ctggatctcg
     3841 gcgccgatcg tcggcgcgtt cacgtagacg atcccgaact cgagctgggt gaccgccttg
     3901 aacacgttcg tgatgtcgtg cgtgtagatc gccgccgaca gcccgtagtc gaccgagttc
     3961 gccatccgga tcgcctcgtc cacgtcgtcg gtgcggatga tccccgtcac gggcccgaag
     4021 atctcctcct gcgcgatccg catgtcgggc gtcatgtcgt cgaacacggt cggggcgatg
     4081 aagttgccct tctcgtagcc gtcgccggtg agctcctcgc caccggtcag cagcgtggcg
     4141 ccctcctgct tcccgatctc gatgtactcg aggatccgct ccttctgcgt ggcattgatc
     4201 accggggcga gctcgacctc ggggtcgaga cctgagccga ccttcatccc ctcaacgcgc
     4261 ttcagcatcc gctcggtgaa ctcctcgcgc gcggcgtcgt ggacgatcag ccgcgaggtg
     4321 gcggtgcagc gctggccgct cgtggcgaac gccccccacg acgccgcctc gaccgccagg
     4381 tccatctcgg cgtcgggcat cacgatcatc acgttcttgg acccgagctc gagcgagcag
     4441 cgcttcatca gcgcgccgca gcgctcgttg atcttccgcc ccacctcgag cgagccggtg
     4501 aacgagatcg cgcggatgtc ggggtgctcc accatggggg caccggcctc ctcgccgaag
     4561 ccgtgcacga ggttgaggac gcccggcgga agcccgacct cctcgaaggc ctggacgagc
     4621 agcgtcgcca gcagcggcgt gtcctcggcg ggcttgaaga tgctcgtgca gccggagacg
     4681 atcgccggcg ccagcttcca cgtggggatc gcgatgggga agttccacgg ggtgatcagg
     4741 cccaccacac cgatcggatg gcgcaccgtc atcgagaact tgtcgcggag ctcggagggg
     4801 atcgtgtggc cctgcatgcg ccgcccctcg ccggccatgt agtggaggaa gtcgatgccc
     4861 tcctggatgt ccccgcggga ctccttcagc gtcttcccca tctcgcgggt catgagctcc
     4921 gaccactcct cgatccgctc ctcgagcagg agcgcgaccc gcaggaggta atcggcgcgc
     4981 tcgggggccg gggtgcgcat ccagtcgtcg taggcgtcct tcgccgcgcg gaccgcggcg
     5041 tccacgtcgt cggcgctcga ccgggggaac gtccccacga cctcgtccat gttcgccggg
     5101 ttgcgggact cgaaggtgtc gccggacgcc gacgggaccc acttcccgcc gatccagttc
     5161 tggtagctct cgatcatctc ctggtcctcc tggctcgcgt cgctccaatc ctactcgcgg
     5221 gcgactggca ctatcatcgt gcccgtgcgg ctcgtgacct acgcgcggga ggggaagcgc
     5281 cgcctcggcg ttctcgtgga cgagacggtc gtggacctcc cggacgcggt cggtcacccc
     5341 gcgttccccg agacgatgga ggccatggtc cgaacgggcc cgggcggcct cggcgtggcc
     5401 ctggaggccc tggagcgcga cggtaccgtc gagcggcacg ccgtcctcgg cgcacgcctg
     5461 ctggcgcctc tcgtgccggg ttccctccgc gacttcctcg ccttcgagga tcacgtgcgc
     5521 gagggcgcga cacggcgcgg ccagcccttc cccgagccct ggcatcggat gcccgtctac
     5581 tacaagggca accaccgctc gatctacggg ccggacgagg aggtcccctg gccccgctac
     5641 acctcggagc tcgactacga gctcgagatc gcctgcatcc tggggggacg ggggcgagac
     5701 ctggacgagg agggggcggc agccctgatc ttcggctaca cgctgatgaa cgactggtcc
     5761 gcgcgcgacg tccagcgcga cgagatggcc tgcggcctgg ggccggccaa ggccaaggac
     5821 ttcgccacct cgctgggtcc gtgcgtggtc acggccgacg agctggaccc caccgacctg
     5881 tggctcaccg cgcgggtgga cggcgagacg tggtcgaagg gcaaccttgg ggccagccgc
     5941 tggacgttcc cccagatgat cgcccacgtg tcgcaggacg aggaggtctg gccgggcgac
     6001 gtctacggat cggggacgtt cgggggcggc tgcggcctcg acctcgaccg gtcgctgagg
     6061 ccgggggcgg tggtcgagct cgaggcggac cggatcggga tcctgcgcaa cccggtcgcc
     6121 gccgcctagg cgaaggccgg gcccagcggc aggatcccct cgatctcgtc cagcacgtcg
     6181 ggggagagct ccacgtcgcc ggcctccgcg ttctgtcgga cgtggtcggg gttgcggctg
     6241 cccgcgatcg ccgccgtcac cccgggttga tggagaatcc aggccagcga gagctgggcc
     6301 ggcgtgatcc ccaaccgctc cgcgatcgga cggaggcgat ccacgacggc caggctccgc
     6361 tcgagcttcc ccggcgcgaa cagggcgtcg tagtaggaca tcccggcgcc ccttccgctc
     6421 cgccagtcgt cgtcggcgaa ctcggtgtcc tcgtcgatcg cgccggtgag gaggccgtag
     6481 gcgagcggcc cgtaggtgac gaccccgatc ccccgctccc cgcaccaccg gatcaggtcc
     6541 cggtgcttga ggtggaggat cgagaactgg ggctggagcg agtccaccgt gcggatcgcc
     6601 tcgcagcgct cgatctgctc gcgaccgaag ttcgagaggc cgagctcgcg gacgagtccc
     6661 tcgtccgcga gctcggccat cgccccccac gtctcctcca ccggcacgtc gtccgacggc
     6721 cagtggagct ggtagaggtc gatcgcgccg atccccagtc gctcgaggct cgccaggcag
     6781 gccctccgga cctcctctgg ccgatggccg gtcccctcgg gcttcggggc caccttcgtg
     6841 gccacgaagg cctcccggcc ctcgatggct ttccccacga gctcctcgga gcggccgctg
     6901 ccgtagccct cggccgtatc gatccacgtg atgccggcat cgaggcccgt gcggatcgcc
     6961 tcgatcacgc ggtcgtccga ctcgttctcg ccccagacgc cgcccccggc ctcccaggcg
     7021 ccgaacccga tcaccgagat ctccgaggcg cccagcggtc gcatgcgcat gggcccgttc
     7081 taccccacga tcaggcgtcg cgacagcggg ggacctcggc cgccgggccg gcgcaggcca
     7141 gcgcgccctc gccccccgat gcgtagtggg cctccacgaa cgcgtgaatc tcgggagggc
     7201 tgaccccgag acaccgctgg agcgagcccc aggccgtcat ggcgaacggc tcctcgagcc
     7261 cgggcagggg cgtcacgatc agcgacgcgt atccctcctc cgcagtcgag ctcacggtcc
     7321 ggagcagcct ctcccgcatc ggctcgggcg cctcgggcga gacccacacc acgaccgccc
     7381 cgtgctccat cgcgtggacg acctccggga ggggctgggg ctcctggtac acggcgcccg
     7441 ccgtggtcgg caccggggag tgcggcccgg aggtcggcgg gatcgaggag tagtcgtcga
     7501 acgcctggtc ctcctcgagg tgggccgtcc ccaagtcggt ggggtgcagg acggggaagc
     7561 acgagctccc tccggccagc cgccacaggt cgcccgcgcg ggccacccgg acgacgcggt
     7621 ggtcggtatg gtcgccgaga acgcgctcgg cgatcagcgt ggccgtctcc tcccccacct
     7681 cgcggtcggt cacgccgacc agcgatcccg aggccgcgac ggtctcctcg aaccgctcgc
     7741 agagctgctc ggccgtgagg aaggcggggt ccacgtagcg ggcggcggcg cggcagtcct
     7801 cctcctccag cgcctcgtag tacgcgcgga gggccccctc gggggtgggg gccccggtga
     7861 tcgggacggg ctcgaacgtg agcgcgcgca cggtccccat cgccagcgtg gcggcgatgg
     7921 ccagggccag cagcacggtg gcgggggtga ggggacggcc gttcatcgtg ccggggacgg
     7981 tacgggcccg ccccggcgag cgtcaacccc gatggtaagg tgacggcgat gggctatcgc
     8041 atcggcgtcc tcggcgcgac gggcctcgtg ggcgaggtcc tcctgagcat cctcgacgag
     8101 cgaggattcc ccgtggacga gctcagggcc ttcgcctccg agcgctcggc gggacgccgg
     8161 atcggctggc ggggcgagga ggtcgtcctc gaggcgccgg ccgagcgacg gttcgccggg
     8221 ctggacctcg tcttcaccgc cgccggctcc gaggtggccc gggagtgggt tccgaggatc
     8281 cgggcccagg gaccggtcgt gatcgacaac tcctcggcgt tccggcagga cccgggcgtc
     8341 ccgctcgtga tccccgagat caacgcccac gcgctccggg accacgacgg cctgatcgcc
     8401 aacccgaact gc
//
LOCUS       DASUTW010000199         4508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_970299, whole genome shotgun sequence.
ACCESSION   DASUTW010000199 DASUTW010000000
VERSION     DASUTW010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970299"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="VF058_06870"
     CDS             <1..1164
                     /locus_tag="VF058_06870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6208068.1"
                     /translation="VGRVEDVVALAVFLIVSGIVGTLLSRAVSQRARAERREREARLL
                     HRVGTRLLSGEEVGDILESFARAVTELFSLARCEISTELTLEPVVVETAKDGVGEPKT
                     VEMSARGRRVGHISMTATDAAGLGAAEREVIQTLANQMGLAIEGVRLGSEATTARVEA
                     ETNRLRAALFSSVTHDLRTPLASITASVTSLQDEDARFTEEDRSELLETIRQEAERLD
                     RLVGDLLDLSRIRAGALVPTKEKSSIEELIEGVVGRLHPLLAAHEIVLRFRPDLPEVP
                     MDIVQIDQVLTNLLENAAKHSPPGTQITISAARWQTMIEVRVIDRGTGVPVEERERVF
                     ELFATGDDDTRGTGMGLAIGQAIVQSHGGRIWIEGTPGGGTTVVFQLPIGEVE"
     gene            1161..1865
                     /locus_tag="VF058_06875"
     CDS             1161..1865
                     /locus_tag="VF058_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007467674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6208069.1"
                     /translation="MTIRVLVVDDDPQILRAVRTSLRARGFDVHTAPNGETALEALSE
                     EGAELVILDLGLPGLDGQEVLRRLRSWSDVPVIVLTVRDAQSEKVAALDAGADDYIVK
                     PFAMEELLARMRAVRRRVSAGASEAVLRFEELEVDLARQAVRLNGELVHLTPTEYRLL
                     ETLVTHPGKLLTHGWLLQRVWGPRYGDESHYLRVYVQQLRKKLRDDPGKPRFILTEPG
                     LGYRWMPEPESDQDAR"
     gene            complement(1909..2571)
                     /gene="hemQ"
                     /locus_tag="VF058_06880"
     CDS             complement(1909..2571)
                     /gene="hemQ"
                     /locus_tag="VF058_06880"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: protein motif:HMM:NF042928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HEX6208070.1"
                     /translation="MSETVTYAYYAAFRRLPSIAEVGDDAVHEWEVLFKEHEDRVRVR
                     GFYSAQGFRPDADLLMWWVAESADAHQDLLIALRRTAIGRHLELIHAFLGAVRPPEFN
                     PDHKPAFMRGEAPRRYLCVYPFVRDADWYLIPSEERAEMLREHGVMGREFPGILPNTT
                     SAFGLGDWEWILAFESDQLHEIVDCIRRLRDAKARRHTKEEVPFVVGIRKELPEVLAD
                     LL"
     gene            complement(2568..3458)
                     /gene="hemH"
                     /locus_tag="VF058_06885"
     CDS             complement(2568..3458)
                     /gene="hemH"
                     /locus_tag="VF058_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX6208071.1"
                     /translation="MTIGVLVMAYGTAAGPDDIERYYTDIRGGRPPSPELLQELRERY
                     AAIGNRFPLTEITEAQAAGLERELGDGFRAYVGMKHSPPFIPETVEKMAADGIERAVG
                     IVMAPHYSRMSIGGYVDRVRKVEAPIEVDVIESWHSHPAYLEVLADRVRRALGTLPED
                     PLVVFTAHSLPARIVDEGDPYPEQLRETADAIAERLGLAHHTTGWQSAGRTPEPWLGP
                     PLEELIPEVAKEHPAVLVVPCGFVSDHLEILYDLDVEAREIAEREGIAFARTESLNAE
                     SDFVAALASIVRDRLASWER"
     gene            complement(3455..4474)
                     /gene="hemE"
                     /locus_tag="VF058_06890"
     CDS             complement(3455..4474)
                     /gene="hemE"
                     /locus_tag="VF058_06890"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013284820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX6208072.1"
                     /translation="MNDRFLRALRREPVDRTPVWFMRQAGRYLPEYRELRGSRDVLTL
                     SRDPEAVVEITLQPLRRMDLDAAILFSDIMVPIVAAGVDVRIESGVGPVIGDPLRASD
                     DLSRIHPFDPAEHAPYVAEAIRLLVKELEVPLIGFVGAPFTLASYLIEGGPSRDHRRT
                     KRLMRDDPSLWERLMDRLAEIVFAHLRAQVEAGAAAVQLFDSWAGALDPETYRRQVLP
                     WSRRILEGLADLDVPRIHFGVGTGELLPAMAEAGAEVVGVDWRVPLDLAWERIGPRAI
                     QGNLDPTACIGSWDACEREAIAVLDRAGGRPGHVFNLGHGVLPETPPDNLARLVDLVH
                     ERTSP"
BASE COUNT          681 a         1502 c         1695 g          630 t
ORIGIN      
        1 gtggggaggg tcgaggacgt ggtcgcgctc gccgtcttcc tgatcgtctc ggggatcgtc
       61 gggaccctcc tctcccgagc cgtctcgcag agggcccgag cggagcgtcg cgagcgggag
      121 gcccgtctcc tccatcgcgt cggcacccgc ctgctgtccg gcgaggaggt cggagacatc
      181 ctggagagct tcgcccgggc cgtcaccgag ctcttctccc tggctcgatg cgagatcagc
      241 acggaactga ccctcgagcc ggtggtggtg gagaccgcga aggacggcgt gggtgagccc
      301 aagaccgtcg agatgagcgc ccggggacgg cgcgtgggcc acatctcgat gaccgcgacc
      361 gacgcggccg gcctgggggc tgctgagcgg gaggtgatcc agaccctggc caaccagatg
      421 ggcctggcca tcgagggtgt ccggctcggg agcgaggcca cgaccgctcg cgtggaggcc
      481 gagacgaacc ggctccgggc cgctctcttc tcctcggtca cccacgacct gaggaccccg
      541 ctggcctcga tcacggcatc cgtcacgagc ctccaggacg aggacgcgcg cttcaccgaa
      601 gaggatcgga gcgagctgct ggagacgatc cgccaggagg cggagcgtct cgaccggctg
      661 gtgggagacc tcctcgatct ctcgcgcatc cgagcgggcg ccctggtccc gaccaaggag
      721 aagtcatcga tcgaggagct gatcgaggga gtggtgggcc ggctgcatcc gctgctggcc
      781 gcgcacgaga tcgtcctccg cttccgccct gatctgccgg aagtgccgat ggacattgtc
      841 cagatcgacc aggtgctgac gaacctgttg gagaacgcgg cgaagcacag ccccccggga
      901 acgcagatca cgatctccgc ggcccgctgg cagacgatga tcgaggttcg ggtgatcgat
      961 cgggggacgg gggtcccggt cgaggagcgc gaacgggtct tcgagctctt cgcgaccggc
     1021 gacgatgaca cgaggggcac gggaatgggc ctcgcgatcg ggcaggccat cgtccagtct
     1081 cacggaggga ggatctggat cgagggcacc cccgggggcg ggaccaccgt cgtcttccag
     1141 cttccgatcg gagaggtcga atgaccatcc gggtgctcgt cgtggacgac gaccctcaga
     1201 tccttcgtgc cgtgcggacg agcctccggg ctcgcgggtt cgacgtgcac accgctccca
     1261 acggcgagac ggcgctggag gcgctctccg aagagggagc cgagctggtg atcctggacc
     1321 tcgggctccc cggcctcgac ggccaggagg tcctccggcg gctccgctcc tggtcggacg
     1381 tgccggtaat cgtgctgacc gtccgcgacg cacagagcga gaaggtggcg gccctcgacg
     1441 ccggagccga cgactacatc gtcaagccgt tcgccatgga ggagctgctg gcgaggatgc
     1501 gggccgtgcg gcggcgcgtc tcggcgggag cgtcggaggc ggtcctgcgg ttcgaggagc
     1561 tggaggtcga tctcgcccgt caggccgtga ggctgaacgg cgagctcgtg cacctcaccc
     1621 ccaccgagta ccgcctgctc gagaccctgg tcacgcatcc tgggaagctg ctcacccacg
     1681 ggtggctgct ccagcgagtg tggggaccga ggtacgggga cgagtcccac tacctgcgcg
     1741 tgtacgtcca gcagctgcgg aagaagctcc gcgacgatcc ggggaagcct cggttcatcc
     1801 tcacggagcc ggggctcggc taccgctgga tgccggagcc cgagagcgac caggacgctc
     1861 gttgacccac ggggtcaccc accggaacca ccgcgacctg ctccgcggct agaggaggtc
     1921 ggccaggacc tccgggagct ccttgcggat gccgacgacg aaggggacct cctccttcgt
     1981 gtgccgtcgg gccttcgcgt cgcggagcct ccggatgcag tccacgatct cgtggagctg
     2041 gtccgactcg aatgccagga tccattccca gtcccccagc ccgaaggcgc tggtggtgtt
     2101 ggggaggatg ccggggaact ctcggcccat caccccgtgc tcgcggagca tctcggcccg
     2161 ctcctcggac gggatcaggt accagtcggc gtcgcgcacg aacgggtaga cgcagaggta
     2221 gcgccgcggc gcctcgcccc gcatgaaggc gggcttgtgg tcggggttga actccggcgg
     2281 ccggaccgcc cccaggaacg cgtggatcag ctcgaggtgg cggccgatcg ccgtgcggcg
     2341 cagcgcgatc agcaggtcct ggtgggcgtc ggccgactcg gccacccacc acatcagaag
     2401 gtcggcgtcg ggacggaacc cctgggccga gtagaacccg cgcacccgca cgcggtcctc
     2461 atgctccttg aagaggacct cccactcgtg gaccgcgtca tcgcccacct cggcgatcga
     2521 cgggagacgc cggaaggccg cgtagtaggc gtaagtgacg gtctcgctca tcgctcccat
     2581 gatgccagac ggtcccggac gatggacgcg agcgcggcga cgaagtcgga ctcggcgttg
     2641 agcgactcgg tccgggcgaa ggcgatgccc tcgcgctcgg cgatctcgcg ggcctcgacg
     2701 tcgaggtcgt agaggatctc caggtggtcc gagacgaacc cgcaggggac gacgaggacc
     2761 gcggggtgct ccttggcgac ctcggggatt agctcctcga gcggcgggcc gagccagggc
     2821 tcgggggtgc ggcccgcgga ctgccagccg gtggtgtggt gggcgagccc gaggcgctcc
     2881 gcgatggcat cggccgtctc gcggagctgc tcggggtagg ggtcgccctc gtccacgatc
     2941 cgcgcgggga ggctgtgggc ggtgaagacg acgagcgggt cctcggggag ggtgcccagg
     3001 gcgcggcgca cgcggtcggc gagcacctcg aggtacgcgg gatgcgagtg ccaggactcg
     3061 atcacgtcca cctcgatggg ggcctccacc ttccggaccc ggtccacgta gcccccgatc
     3121 gacatcctcg agtagtgggg ggccatcacg atccccacgg cccgctcgat gccgtcggcc
     3181 gccatcttct ccacggtctc ggggatgaag gggggcgagt gcttcatccc gacgtaggcc
     3241 cggaagccgt cgcccagctc ccgctccagg cccgccgcct gggcctcggt gatctcggtg
     3301 agcgggaagc ggttgccgat cgcggcgtag cgctcgcgca gctcctggag cagctcgggg
     3361 ctgggcgggc ggccgccgcg gatgtccgtg tagtagcgct cgatgtcgtc ggggcccgcg
     3421 gcggtcccgt aggccatcac gagcacgccg atcgtcatgg ggaggtcctc tcgtggacga
     3481 ggtcgacgag ccgagccagg ttgtcgggcg gcgtctcggg gaggacgccg tggccgaggt
     3541 tgaagacgtg gccggggcgg ccgccggcgc ggtcgagcac ggcgatcgcc tcccgctcgc
     3601 aggcgtccca cgagccgatg caggcggtgg ggtcgaggtt gccctggatg gcccgagggc
     3661 cgatccgctc ccaggccaga tcgagcggga cacgccagtc cacgccgacc acctcggcgc
     3721 ccgcctcggc catggccggg agcagctcgc cggtccccac gccgaagtgg atgcgcggca
     3781 cgtcgaggtc tgccaggccc tcgaggatgc ggcggctcca gggcagaacc tgccggcggt
     3841 aggtctcggg gtcgagggcg cctgcccagg agtcgaagag ctggacggcc gcggcgccgg
     3901 cctcgacctg ggcccggagg tgggcgaaga cgatctcggc caggcgatcc atcaggcgct
     3961 cccagagcga ggggtcgtcg cgcatcaggc gcttcgtgcg gcggtggtcg cgcgagggac
     4021 cgccctcgat caggtaggag gcgagggtga agggggcgcc gacgaacccg atcagcggca
     4081 cctcgagctc cttgacgagg aggcggatcg cctcggccac gtacggggcg tgctcggcgg
     4141 gatcgaaggg atggatgcgc gagaggtcgt ccgaggcgcg gagggggtcg ccgatcaccg
     4201 gccccacccc cgactcgatc ctcacgtcca ccccggccgc cacgatcgga accatgatgt
     4261 ccgagaagag gatggccgcg tcgaggtcca tccgccgcag cggctggagc gtgatctcga
     4321 ccacggcttc gggatcgcgc gagagcgtga ggacgtcgcg gctgccgcgg agctcccggt
     4381 actcggggag gtagcggccg gcctgccgca tgaaccagac cggcgtgcgg tcgacgggct
     4441 cgcgccggag ggctcggagg aagcggtcgt tcacgccctc aggatacgga cgcgccgggg
     4501 acgaaggc
//
LOCUS       DASUTW010000200         4633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_970389, whole genome shotgun sequence.
ACCESSION   DASUTW010000200 DASUTW010000000
VERSION     DASUTW010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970389"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..120)
                     /locus_tag="VF058_06895"
     tRNA            complement(47..120)
                     /locus_tag="VF058_06895"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(84..86),aa:Pro,seq:tgg)
     gene            172..242
                     /locus_tag="VF058_06900"
     tRNA            172..242
                     /locus_tag="VF058_06900"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:204..206,aa:Gly,seq:tcc)
     gene            319..723
                     /locus_tag="VF058_06905"
     CDS             319..723
                     /locus_tag="VF058_06905"
                     /inference="COORDINATES: protein motif:HMM:NF016757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEX6208073.1"
                     /translation="MARQRGALPLAVLIAATFIAGVTTAVIRVTEDEAPQAVPESPSP
                     TATSPSPEPTDEPSPEPTEEPTEEPTEEPTEEPTEEPTEEPTEEPTVDGDDGGGGGLA
                     ETGTESLPFLLGGAVMIGLAGALWRLSRRPVD"
     gene            complement(725..1465)
                     /locus_tag="VF058_06910"
     CDS             complement(725..1465)
                     /locus_tag="VF058_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX6208074.1"
                     /translation="MATIAVLSDLHSNVEALDAVVAAARHRDATGFIVCGDLVGYGAD
                     PGAVLERVFDLPLRSVVAGNHDLAAVDRFSLDWFNDVAAEALRWTSEQLTPDARDTLA
                     RLEPREPSTEGLVVHGSVVEPASEYLMPGEDAAAARSFGHEPFDRCFFGHTHVPTMFE
                     QREDGGVRSAPIRSEGRLTLTVGHRYLLNPGSVGQPRDGDPRAAFMILRDGTVEWVRV
                     PYDAEAAGEKIREAGLPGVLADRLLVGR"
     gene            1609..2010
                     /locus_tag="VF058_06915"
     CDS             1609..2010
                     /locus_tag="VF058_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208075.1"
                     /translation="MIATSRRRALWAALAALVLTGTLFSIPASAGDDPRVERRGGCSA
                     SSRWKMKAKKDDGRIEVEFEVDQNVNGQRWRVVMKRNGTRFFKGVRRTQPPSGSFSVE
                     RRTPDGPGRDRFRARARNLRTGEVCRGRVAI"
     gene            2048..2120
                     /locus_tag="VF058_06920"
     tRNA            2048..2120
                     /locus_tag="VF058_06920"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2081..2083,aa:Arg,seq:tct)
     gene            complement(2296..2856)
                     /locus_tag="VF058_06925"
     CDS             complement(2296..2856)
                     /locus_tag="VF058_06925"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HEX6208076.1"
                     /translation="MRRSILVLAMLTAATAAGCADDGGEPGFTPPPSPTGVPTASPTP
                     SPTAGTHVVRVFFGAPDAAETDCAAVVAVERTTEGADVLHDAMEALLEGPTSGERAQG
                     ISSFFSRKTRGMLDAVRIEDGVAHVDFRDFSRVIPNASTSCGSAALLAQLDATATQFP
                     TVREARYSFEGSEEAFYEWLQMSVPT"
     gene            complement(2954..4402)
                     /locus_tag="VF058_06930"
     CDS             complement(2954..4402)
                     /locus_tag="VF058_06930"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020556461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6208077.1"
                     /translation="MRNQMLIAGSWTDGRGSESMAVTSPVTGEGLGEVPVATPEEIDE
                     AVGAARGAVEALDRMTVFERAERLHRVAALMRERKEELARELTLEHGKPYKAEAVDEI
                     EESAENFEVAAEDIKRVNTEVLPSTDRNKRMFTLRKANGVYATITPWNFPFVIPSELL
                     APGLAAGNAMILKPSEHTPLIGARMAEIITEAGFPEGAISLVFGAGETGRTLVSHPGV
                     DAIAFVGHNSTAEAIVRAAGLKRTLMEASGNGPVIVLDDADLDEAAAMATFGATYVSG
                     QCCVATERILVSESVHDDFLERLVKEAEAAVLGDPFDDATIMGPLNNADVAEKMDRHL
                     EDAREKGVAILTGGRRADGFPTDLYYELTVADGVGADTLLFREETFGPVLPVTAFRDD
                     EEAIAMANDSDLGLQAAVFTSSLKRAFRFIDRIRAGNIVVNDTTDYWEAHQPFGGASG
                     TKTGWGRVGGKYAIEDMSDLRTVIIDWKKTKD"
     gene            4489..4562
                     /locus_tag="VF058_06935"
     tRNA            4489..4562
                     /locus_tag="VF058_06935"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4523..4525,aa:His,seq:gtg)
BASE COUNT          657 a         1643 c         1628 g          705 t
ORIGIN      
        1 gccttcgtcc atagtgacga tccggagccc ggctcctcat cgccggtcgg ggcgggcgga
       61 ttcgaaccgc cgacctcgtg ctcccaaagc acgcgcgctg ccaagctgcg ccacgccccg
      121 ctcgctcgat tctacggccc gcagacccga ggccggtaca ctagacgcgt cgcgggtgta
      181 gctcaatggt agagccccag ccttccaagc tggttacggg ggttcgattc ccctcacccg
      241 ctcgatccgc tcggcaacgg cgccgacgct cctctccttc gggttagaat ccgcggggca
      301 ctggggggaa ggagatccgt ggcacgacag cgaggcgcgc tgccgttggc ggtgctcatc
      361 gcggcgacct tcatagcggg cgtgacgacg gcggtgatcc gcgtcaccga ggacgaggcg
      421 ccgcaggccg ttcccgagag cccttccccc accgccacct ccccatcccc cgagcccacc
      481 gacgagccct cgcccgaacc gaccgaggag ccgaccgagg agccgaccga ggagcccacc
      541 gaggagccga cggaagaacc tacggaagag cccaccgagg agccgacggt cgacggcgac
      601 gatgggggcg gcggcgggct cgccgagacc gggaccgagt cgctcccctt cctcctggga
      661 ggcgccgtga tgatcggcct ggccggcgcg ctctggcgcc tctcgcgtcg tcccgtcgac
      721 tgactcagcg ccccacgagc agacgatccg ccaggacccc gggcagcccc gcttccctga
      781 tcttctcccc cgccgcctcc gcgtcgtagg gcacgcggac ccactccacc gtcccgtccc
      841 ggaggatcat gaacgcggcc cggggatccc cgtcgcgagg ctgccccacg ctcccggggt
      901 tgaggaggta ccggtgaccg accgtcagcg tgagccgtcc ctcgctccgg attggggcgc
      961 tccgaacgcc tccgtcctcc cgctgctcga acatcgtggg gacgtgcgtg tggccgaaga
     1021 agcaccggtc gaagggctcg tgcccgaagc ttcgggcggc ggcagcgtcc tcgccgggca
     1081 tcaggtactc actcgcgggc tcgacgacgg agccgtgcac gacgaggccc tcggtcgagg
     1141 gctcgcgcgg ctccaaccgg gccagggtgt cgcgagcgtc gggggtgagc tgctcgctcg
     1201 tccagcgaag ggcctcggcc gcgacgtcgt tgaaccagtc gagggagaag cggtccacgg
     1261 cggccaggtc gtggttcccg gccacgaccg accgaagggg gaggtcgaag actcgctcca
     1321 gcaccgcgcc gggatcggcc ccgtatccca cgaggtcgcc gcacacgatg aagccggtgg
     1381 cgtcacggtg gcgcgcagcc gcgacgaccg cgtcgagcgc ctccacgttc gagtgaaggt
     1441 cggagaggac ggcgatcgtc gccatccccc aagtctgccc gccttcgcgg ctccccgcac
     1501 gaggggctga aggaaccctc aggcgcgctt caggaccgcc tcaggcggcg gccgcatgct
     1561 cggactcgac cggcggccga ggggccgccc acgatcgagg aggacgcgat gatcgcgacg
     1621 tcgaggagac gggcgctgtg ggccgcgctg gccgcgctcg tcctgacggg cacgctgttc
     1681 tcgatcccgg caagcgccgg ggacgatccc cgcgtggagc gcaggggcgg ctgctcagcg
     1741 agcagccgat ggaagatgaa ggcgaagaag gacgacggcc ggatcgaggt cgagttcgag
     1801 gtggaccaga acgtcaacgg gcagcggtgg cgcgtggtca tgaagcgcaa tgggacgcga
     1861 ttcttcaagg gcgtccgcag gacccagcct ccgagcgggt cgttcagcgt ggagcgtcgc
     1921 acccccgatg gtccggggag ggaccgcttc cgggcccggg ccaggaacct ccgcacgggc
     1981 gaggtctgcc gagggcgcgt cgcgatctga cgcggccggg aggtccgaag gatctacact
     2041 ggtggcagcg ccggtagctc aggggacaga gcggctgcct tctaagcagc gggacggggg
     2101 ttcgaatccc tcccggcgcg cggatgcggt cacgcggagc ggcgcgctcc gcgtgaccgc
     2161 atcccgctcc gcacccgatg ctcgagcacg cccttccgcg atgtgcgttc gggcttcttg
     2221 tgcttccgct tgcgggtctc agagcgagcg ggacggttcc ctcggcgacc tcctcctcga
     2281 tacatctccc tcacctcacg tgggcacgct catctggagc cactcgtaga aggcctcctc
     2341 gctcccctcg aacgagtacc tcgcctcccg tacggtcggg aactgggtcg cggtcgcgtc
     2401 gagctgggcc aggagggccg cgctgccgca cgaggtcgac gcgttcggga tcacccgaga
     2461 gaagtcccgg aagtcaacgt gggccacgcc gtcctcgatg cgcacggcgt cgagcatccc
     2521 gcgagtcttc cggctgaaga agctgctgat gccctgggcc cgctcccccg aggtcgggcc
     2581 ttcgaggagg gcctccatgg cgtcgtggag gacgtcggct ccttcggtcg tccgctcgac
     2641 cgcgacgacc gccgcgcagt cggtctcagc tgcgtcgggc gcgccgaaga acacccgcac
     2701 gacgtgggtc cccgcggtgg gcgagggtgt ggggctcgcc gtggggacgc cggtggggct
     2761 gggaggcggc gtgaacccgg gctcgccccc gtcatccgcg catccggcgg ccgtcgccgc
     2821 ggtcagcatc gcgagcacga ggatcgatct gcgcatgcgc aacctccacg atgactgtac
     2881 gcggggcggg agcgtcggct tcggggccgt tcggaccgct tcccgcgggt ccctccgccg
     2941 atggggccgc gggtcagtcc ttcgtcttct tccagtcgat gatgaccgtg cgcaggtcgg
     3001 acatgtcctc gatcgcatac ttcccgccga cccttcccca cccggtcttc gtgccggagg
     3061 cgcctccgaa gggctggtgc gcttcccagt agtccgtggt gtcgttcacc acgatgttgc
     3121 cggcccggat ccggtcgatg aaccggaacg ctcgcttgag gctcgaggtg aagacggcgg
     3181 cctgcagccc gaggtccgag tcgttggcca tggcgatggc ctcctcgtcg tctcggaagg
     3241 cggtgacggg gagcacgggc ccgaaggtct cctctcggaa gagcagggtg tcggcgccga
     3301 cgccgtcggc cacggtgagc tcgtagtaca ggtccgtggg gaacccgtcc gcccgccgtc
     3361 cacccgtgag gatcgcgacg cccttctcgc gtgcgtcctc gaggtgccgg tccatcttct
     3421 cggccacgtc ggcgttgttc agcggcccca tgatcgtggc gtcatcgaac ggatctccga
     3481 gcacggcggc ctccgcctcc ttcaccagcc gttccaggaa gtcgtcgtgg acggactcgg
     3541 agaccaggat ccgctcggtc gccacgcagc actgccccga gacgtacgtc gcgccgaacg
     3601 tggccatggc cgccgcctcg tccaggtcgg cgtcgtcgag cacgatcacc gggccgttgc
     3661 ccgaggcttc catcagggtg cgcttcaggc ccgccgcgcg gacgatggcc tccgcggtgc
     3721 tgttgtggcc cacgaacgcg atcgcgtcca cgccggggtg ggagacgagc gtccgtcccg
     3781 tctcccccgc gccgaacacg agcgagatgg ccccctcggg gaagccggcc tcggtgatga
     3841 tctcggccat ccgggcgccg attagcggcg tgtgctcgga gggcttgagg atcatcgcgt
     3901 tcccggcggc caggccgggg gcgagcagct cggacgggat cacgaagggg aagttccacg
     3961 gcgtgatcgt tgcgtacaca ccgttcgcct tccggagggt gaacatccgc ttgttgcggt
     4021 cggtggacgg gaggacctcg gtgttgacgc gcttgatgtc ctctgccgcg acctcgaagt
     4081 tctcggccga ctcctcgatc tcgtcgacgg cctcggcctt gtagggcttc ccgtgctcga
     4141 gcgtgagctc ccgggcgagc tcctccttcc gctcgcgcat cagcgccgcc acgcggtgga
     4201 ggcgctccgc gcgctcgaag accgtcatgc ggtcgagcgc ctcgacggcg ccgcgggcgg
     4261 cccccaccgc ctcgtcaatc tcctcggggg tggcgaccgg gacctcgccc agcccctccc
     4321 ccgtcaccgg gctcgtcacc gccatcgact cggagccccg cccgtctgtc cacgagcccg
     4381 cgatcagcat ctggttgcgc atggcatcct cctcggggac aggctacccg cgcgggtcgc
     4441 tcttcacgaa ggggcgaggg aggccactgc tacaatcgat gcagcgtggt ggccgtagct
     4501 caagtggtta gagcgtcggg ttgtggtccc gaaggttggg ggttcgagtc ccctcggtca
     4561 ccctctcgtg ggcgggcatg agggcgcccg ctacactgtt cctgcggggc cgttagctca
     4621 aactggcaga gca
//
LOCUS       DASUTW010000201        10554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_190611, whole genome shotgun sequence.
ACCESSION   DASUTW010000201 DASUTW010000000
VERSION     DASUTW010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10554
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190611"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..1036
                     /gene="glpX"
                     /locus_tag="VF058_06940"
     CDS             50..1036
                     /gene="glpX"
                     /locus_tag="VF058_06940"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005514628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX6208078.1"
                     /translation="MTEKPDRNLAMELVRTTEAAAMAAGRWAGRGEKESSDQAAVDAM
                     RLMLDTVSMDGIVVIGEGEKDEAPMLYNGEKVGSGDGPLVDVAVDPIDGTRLLSKGMG
                     NALSVVAVSERGTMFDPGAAVYMEKVAVGPEAAEAIDLEAGTAENIARVARAKDLDVE
                     DVTVVVLDRERHEGLVEEIREAGARIRFITDGDVAGAIAAASQRPGADLLMGIGGTPE
                     GVIAAAALKCLGGAIQGKLYPRNDDERSALVDAGYDLDRILTTEDLVSGEEVFFAATG
                     ITDGFLLKGIRYRQREAVTFSMVMRSKSGTIRLVEGRHQLEKLMRFSEIEYD"
     gene            1039..1317
                     /locus_tag="VF058_06945"
     CDS             1039..1317
                     /locus_tag="VF058_06945"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEX6208079.1"
                     /translation="MTFEEAVIAVVRALGPGEVATYGEVAEEAGHPGAARAVGNVMAS
                     SEGLPWWRIVRSDGSLAVDKGGEQARRLQAEGVEVRGGRVRPQRSCSV"
     gene            complement(1296..1517)
                     /locus_tag="VF058_06950"
     CDS             complement(1296..1517)
                     /locus_tag="VF058_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEX6208080.1"
                     /translation="MDELEAAVRVLEEEGEPLHWTVIQDLALKRGYLDPFTRKDIRRR
                     LIAALAAAAKRPDHPIRKTGKGVYTLQER"
     gene            complement(1517..2524)
                     /locus_tag="VF058_06955"
     CDS             complement(1517..2524)
                     /locus_tag="VF058_06955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6208081.1"
                     /translation="MRLGLALPHYDFSFADGRPVTWERLRSWGTAAEEMGFDSIWISD
                     HFFLSLARYGGRDEPFGTPEALTALAALATTTDRVRLGTLVLSAGFRHPAIVAKAAAV
                     IDLLSGGRFDLGIGAGWYEDEYRAFGYDYEDARGRFDVLQESLIVLGELLGDGPATWR
                     GDRFSLDGAYNRPAPVQRPRPPIWIGGKGGPRLQRLVARHGDGWNSVWAWSPQRYRGK
                     VDELRATCEREGRDPASVRLSVGLYLAIGRDERGVRDVLERYAERVPGGVPASEDPAE
                     WRSERLAGTPPEVLERLGAFAELGVEEVIVSPASVPFAVHDEDALALFAEAVVPAARD
                     L"
     gene            complement(2558..3289)
                     /locus_tag="VF058_06960"
     CDS             complement(2558..3289)
                     /locus_tag="VF058_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6208082.1"
                     /translation="MDCAEVRGMLPEHALGTLGASDAARVREHLGACAGCRKETGELM
                     EGVEVAALATAAQPPPELEDRVVAAVARAASRRAGRRRLPVAGLLAAALLLALGWGAA
                     MAGRAGRLGNEAEMARRQAAQAAGDFGEFVGDLSGGGAREAVLRSTSWAGAGGRAVVF
                     DSDVSGASDWILVVVGGLPEEDGPYRAKLRGEGGDLRVGRLWPSAEGRLAAYALFGAL
                     PEDREDVVVLDADGAVVLKGRLEAR"
     gene            complement(3289..3864)
                     /locus_tag="VF058_06965"
     CDS             complement(3289..3864)
                     /locus_tag="VF058_06965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6208083.1"
                     /translation="MGSTDAPDGPLTPEAERGLVRRLISGDEEAVRILYGRFAGPVYG
                     LGLRLLGSPEAAQELTQDTFLAVWRKAPQYDPARGRLSTWIMVIAHNLGVDRLRREAR
                     RPVALVEDMETVGGEDDETEARILDRVATLEILSVLSAAERRLLGLAYYRGWTAREIS
                     LHDSIPLGTVKTRLRSALIKLRRAHGWKREL"
     gene            complement(3909..4592)
                     /locus_tag="VF058_06970"
     CDS             complement(3909..4592)
                     /locus_tag="VF058_06970"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6208084.1"
                     /translation="MDTKALEIHKALADNTRFRLYRHLRLMDRPVSVREMSRRLSLHP
                     NTLRPHLRRLEEAGLVRHELRRSDGVGRPQMLYVGVEPAGDEGRDFRLLSEMLLGTVQ
                     TRRQLERATALARGWGEYLVSRDAPKPGVRLPDRKNLAVLQSAMDRAGFEPRFRRQGT
                     SVEVTFRRCPFRELVDEHREVVCGLHRGLLEGMLGGLRPPLELRELKPFVERGVCRLR
                     AAPPRRKRG"
     gene            4664..5758
                     /locus_tag="VF058_06975"
     CDS             4664..5758
                     /locus_tag="VF058_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010047745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX6208085.1"
                     /translation="MITEQDVRHALHRALDPSIEKPLDDLGAVEAVETDRDRVRVSIR
                     VPIPGDDENVRRSVEEALAGLGAHLEVRVSEMSPEQRAEMVGRLRGGKTREETVFFSE
                     GPTEVVAVASGKGGVGKSSVTANLAASLAAEGQRVGILDADVWGFSIPRMMGVSGQPA
                     GTSDMIVPPRAHGVKIISMGFFVSDETPVIWRGPMLHKAIQQFLGDVYWGELDALFVD
                     LPPGTGDVSISLASFLPRGAMLVVTTPQEAAAKVAERAGRMAQHPNIDLEVLGVVENM
                     AGFVCPHCGEITPLFGEGGGARTAEALDVPLLGSVPIQVSLREGSDEGTPVVVSEPDS
                     PAGAAFREIAGELGTRIRSPRGKPLSLRET"
     gene            complement(5739..6749)
                     /locus_tag="VF058_06980"
     CDS             complement(5739..6749)
                     /locus_tag="VF058_06980"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX6208086.1"
                     /translation="MDALATLSWPVLDRIDLGPLAISPHGIGIAVGYLLGAWWMMREG
                     PKRGLREEHISTIILWALVGAIVGARFFYVLGHLDEFDSLTDMLAIWRGGISLVGGIF
                     GAIGFAVPLMRRYGYRFGQVMDSAAVGLAFGIAVGRIGDLVIGDHLGKPTDFLIGWRY
                     TSGTLPGPYQAAGPDTWIASLAGPHCQVIRPDGAELYEGTCDLTGAVLAQGPGVHQTA
                     LYDILIAGALFAFLFWLSRKPRREGVLIAAFGIWYGAGRLLTDFLRVDKTWPLGLTGS
                     QWASVGVIAVAVAVLVRFALRPGPAAVTIPEAEDSSSALASGPTTSFTPPPEPRLRSP
                     GD"
     gene            complement(6760..7362)
                     /locus_tag="VF058_06985"
     CDS             complement(6760..7362)
                     /locus_tag="VF058_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX6208087.1"
                     /translation="MRRRRRRVGRALGLAGAALGLAAITGSVRGRELDERLYKQMNTN
                     LEHPALDRLLSEVTELGSLYASLGAGAVLATSGSRRAAARAVGAAASMWALGQALKRA
                     YRRTRPFDSTAPGRLLIGKPRGTSWPSSHPAVFLTFALVAARRLDLPGPARSALVALA
                     GAVGLSRVYLGVHYPSDVAGGLLLGRAMADVWTAPDPDVR"
     gene            complement(7359..7802)
                     /locus_tag="VF058_06990"
     CDS             complement(7359..7802)
                     /locus_tag="VF058_06990"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6208088.1"
                     /translation="MSTIRDAVVVDATPEEVWRVIADPRNLPRWNRHLKAVIGAPDGE
                     LQTGDSYRVELGLLGVSAEVRASVKRLERPTYSEVALSGPIEGVVRTFVRPIGARRSR
                     VEHEVEYRFPGGALGRAAARAVRLMGASLILRRGLRAQKEMVEGR"
     gene            complement(7799..8737)
                     /locus_tag="VF058_06995"
     CDS             complement(7799..8737)
                     /locus_tag="VF058_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6208089.1"
                     /translation="MIPEEARRVLRDGVLLYAAAAGPDAPHLTPMVFVLAEDRLWATT
                     ARRSRKVRLWRARPDGGGLVRVGDRAVSFRGRVDLYDLLDPTTWGRSLLRSQLLTRAA
                     LGFSRKNARFFAGYARDARRVPFGWTPPGRVFLSIALRDGVLLDLSEGEVLRSWGRLD
                     GGLIGATSFRRGPARPAPDAGVPGDVRDALGDEGRGALALDDAPVLPVRWRRSEEEGA
                     YYAVVPASFLERTGAADRAPATLVLDHLSRWRASRMLGMQLRGEGRVYVPGRLRSGEA
                     SLAVRSGATEGDAVVRLRPDRVVWWRGWASGSLGGR"
     gene            complement(8734..9354)
                     /locus_tag="VF058_07000"
     CDS             complement(8734..9354)
                     /locus_tag="VF058_07000"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX6208090.1"
                     /translation="MDAVAVAVAWIGPLALAGAWAAVRIGGVSIWAAMAWTVGPLGAL
                     ALLSGGVEAGGGLLEALIGLGAGLAFYAATATFLFLARGWRILRRQAHELYARRRGLS
                     LGAVLAIACLVVAPAEESLWRGLIQGRLSGALGEAPGAALAWALYVLANLVSGSVPIV
                     LGAVVGGAAWTALALWTGGILASVACHVVWTGLMILRPPVGEGSEP"
     gene            complement(9356..10144)
                     /locus_tag="VF058_07005"
     CDS             complement(9356..10144)
                     /locus_tag="VF058_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208091.1"
                     /translation="MEGASGVIALVLAETAAGSAALLWLTGLWGNVKRGFFILTTATV
                     LACALLAALSANAGATEAGSGGARLVVVLALALSALSALALIALILRRMAAGRVLGLV
                     SIPVGVALLVAMASVAEAGMAMALVGLLAGAAFMGAVTVGLLLGHWYLVDRSLARDHI
                     QRMAVVLIGAVLVEAVAVMIAAVAGDVTPTEGFGLFLTVGGAAFHLAIVLGMVAITAL
                     IAFLIRAALRETRPRAVQAATGFFYLAVITAFTAELAAKVRFLG"
     gene            10166..10552
                     /locus_tag="VF058_07010"
     CDS             10166..10552
                     /locus_tag="VF058_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEX6208092.1"
                     /translation="MLQPGTSATVAETAAEEQTAERIGSGDVPVYATPMLLALAERAA
                     VAAIKTALEPGMTSVGTRVELDHLAPTPAGSRVEATATLKEVDGRRLVFSFEARDPGG
                     VVARGTHVRVLVRRGPFVEGARSRVV"
BASE COUNT         1545 a         3923 c         3866 g         1220 t
ORIGIN      
        1 gcatcctcca cgagggagcg tcccccacga gtctcttgga ggagctgcga tgacggagaa
       61 gcccgatcgg aacctggcga tggagctggt gcggaccacc gaggccgcgg cgatggccgc
      121 cggccgctgg gccggccgag gcgagaagga gtcgtcggac caggcggcgg tggacgcgat
      181 gcgcctgatg ctcgacaccg tctcgatgga cggcatcgtc gtgatcggcg agggcgagaa
      241 ggacgaggcg ccgatgctct acaacggcga gaaggtgggg agcggggacg ggccgctggt
      301 cgacgtcgcg gtggacccga tcgacgggac ccgcctcctc tccaagggga tggggaacgc
      361 gctctcggtc gtggccgtgt ccgagcgggg cacgatgttc gacccggggg ccgcggtgta
      421 catggagaag gtcgccgtgg gacccgaggc ggcggaggcc atcgacctcg aggccggcac
      481 ggccgagaac atcgcccgcg tagcgagggc gaaggacctg gacgtggagg acgtcaccgt
      541 cgtcgtcctg gaccgggagc ggcacgaggg cctggtcgag gagatccgcg aggcgggagc
      601 ccgcatccgc ttcatcaccg acggcgacgt ggcgggcgcg atcgccgcgg cgtcccagag
      661 gcccggcgcc gacctcctca tggggatcgg cggcacgccg gagggcgtga tcgcggcggc
      721 cgccctgaag tgcctcggcg gcgcgatcca gggcaagctg tacccgagga acgacgacga
      781 gcggagcgcc ctcgtcgacg ccgggtacga cctcgatcgg atcctcacca ccgaggacct
      841 cgtctccggc gaggaggtct tcttcgcggc gacgggcatc accgacggct tcctgctgaa
      901 ggggatccgc taccggcagc gcgaggccgt gacgttctcg atggtgatgc ggtcgaagtc
      961 gggcacgatc cggctggtcg agggccgaca ccagctcgag aagctgatgc gcttctcgga
     1021 gatcgagtac gactgaggat gaccttcgag gaagcggtga tcgccgtcgt ccgcgcgctc
     1081 ggccccggcg aggtcgccac ctacggcgag gtggccgagg aggccggaca cccgggcgcg
     1141 gcccgcgcgg tgggcaacgt gatggcgtcc agcgaggggc tcccctggtg gcggatcgtc
     1201 cgctcggacg gcagcctcgc ggtggacaag gggggcgagc aggctcggcg gctccaggcc
     1261 gagggcgtcg aggtgcgcgg cgggcgggtg cggcctcagc gctcctgtag cgtgtagacg
     1321 cccttccccg tcttccggat cgggtggtcg ggacgcttgg cggcggcggc gagggcggcg
     1381 atcagccgcc tgcggatgtc cttccgggtg aacgggtcga ggtagccacg cttgagggcg
     1441 aggtcctgga tcaccgtcca gtggaggggc tccccctcct cctccaggac gcggacggcg
     1501 gcctccagct cgtccatcag aggtcgcggg cggcggggac gacggcctcg gcgaagagcg
     1561 ccagcgcgtc ctcgtcgtgc acggcgaacg ggaccgaggc cggcgacacg atcacctctt
     1621 ccacgccgag ctcggcgaag gcccccaggc gctcgaggac ctcggggggc gtgccggcca
     1681 gccgctccga ccgccactcg gcgggatcct cgctggccgg cacgcccccg ggcacgcgct
     1741 cggcgtatcg ctccaggacg tcccgcaccc cgcgctcgtc gcggccgatc gccaggtaga
     1801 ggccgaccga gagccgcacc gaggccgggt cgcgcccctc tcgctcgcag gtggctcgga
     1861 gctcgtccac cttcccccgg taccgctgcg gcgaccaggc ccacacgctg ttccagccgt
     1921 ccccgtgccg ggccaccagg cgctggagcc gcggaccgcc cttcccgccg atccagatcg
     1981 ggggccgcgg acgctggacc ggggccgggc ggttgtaggc gccgtccagc gagaaccggt
     2041 cgccacgcca ggtcgccggg ccgtccccca ggagctcgcc gagaacgatc agcgactcct
     2101 gcaggacgtc gaaccggccc cgggcgtcct cgtagtcgta gccgaaggcc cggtactcgt
     2161 cctcgtacca accggccccg atcccaagat cgaagcgccc gcccgagagc aggtcgatga
     2221 cggcggcggc cttggccacg atggcggggt ggcggaagcc cgcgctcagc acgagcgtgc
     2281 ccagccgcac acggtccgtg gtcgtggcca gcgcggcgag ggcggtgagg gcctcgggcg
     2341 tgccgaaggg ctcgtcccgc cctccgtagc gggccagcga gaggaagaag tggtccgaga
     2401 tccagatcga gtcgaatccc atctcctcgg cggcggtccc ccaggaacgc aggcgctccc
     2461 acgtcacggg ccgtccgtcc gcgaacgaga agtcgtagtg gggaagggcg aggcccagcc
     2521 tcatgcgggc catcttatcg gtcggccgga cggcgtctca gcgggcctcg aggcggccct
     2581 tcagcacgac ggcgccgtcg gcgtcgagga cgacgacgtc ctcccggtcc tccggcagcg
     2641 cgccgaacag cgcgtaggcg gcgagcctgc cctccgcgga cggccacagc cgccccacgc
     2701 ggaggtcgcc gccctcccct cggagcttgg cccggtaggg gccgtcctcc tcgggcagcc
     2761 cgcccaccac cacgaggatc cagtcggagg cgccggagac gtcgctgtcg aacacgaccg
     2821 ccctcccgcc ggcccccgcc cacgacgtgg accggagcac ggcctcgcgg gcccccccgc
     2881 cggagaggtc tcctacgaac tccccgaagt cccccgcggc ctgcgcggcc tgccggcgcg
     2941 ccatctcggc ctcgttcccg agacgccccg cccgccccgc catcgccgcg ccccacccga
     3001 gcgccaggag gagcgcggcg gccaagaggc cggcgacagg gaggcggcgg cggccggccc
     3061 gacggctggc ggccctcgcg accgccgcca cgacgcggtc ctcgagctcg ggcgggggct
     3121 gggcggcggt cgcgagggcc gcaacctcca cgccctccat cagctccccc gtctccttgc
     3181 ggcacccggc gcaggcgccg agatgctccc gcacccgagc ggcgtcgctc gccccgagcg
     3241 tcccgagggc gtgctcgggg agcatcccgc gtacctcggc gcagtccatc agagctcccg
     3301 cttccatccg tgggcccggc gaagcttgat cagcgccgac cgcaggcggg tcttcacggt
     3361 ccccagcggg atcgagtcgt gcagcgagat ctctcgcgcc gtccagcccc ggtagtaggc
     3421 gaggcccagg agcctccgct cggcggcgga caggacggag aggatctcca gggtggccac
     3481 gcggtccagg atccgagcct cggtctcgtc gtcctcccct cccacggtct ccatgtcctc
     3541 gaccagggcc accggccggc gggcctcgcg ccgcaggcgg tccacgccca ggttgtgggc
     3601 gatcaccatg atccaggtcg agagccggcc ccgggccggg tcgtactggg gcgccttgcg
     3661 ccacacggcc aggaaggtgt cctgcgtgag ctcctgggca gcctccgggc tccccagcag
     3721 ccgcaggccc aggccgtaga ccggccccgc gaaccgaccg tacaggatcc tcaccgcctc
     3781 ctcgtcaccg gagatcagcc gccgcacgag ccctcgctcg gcctcgggcg tcagcggccc
     3841 gtcgggcgcg tcggtcgatc ccataccagt gatacgcgag ggcggcaggc ctggattgcc
     3901 gggcaccgtc agccgcgctt cctgcggggg ggcgctgcgc ggagcctgca gacgccccgc
     3961 tccacgaacg gcttcagctc gcgcagctcg aggggcgggc gcaggccccc cagcatcccc
     4021 tcgaggaggc cgcggtggag cccgcacacc acctcgcggt gctcgtccac gagctcccgg
     4081 aaggggcacc ggcggaacgt cacctcgacg gaggtcccct gcctccggaa cctcggctcg
     4141 aagcccgctc gatccatcgc cgactgcagg acggccaggt tcttccggtc ggggaggcgg
     4201 acgccgggct tcggcgcgtc ccgcgagacg aggtactcgc cccagccgcg agccagcgcg
     4261 gtggcccgct ccagctggcg ccgggtctgg accgtcccca ggagcatctc cgagagcagg
     4321 cggaagtctc ggccctcgtc gccggccggc tccacaccca cgtacagcat ctgggggcgg
     4381 cccacgccgt cgctcctgcg cagctcgtgg cggacgagcc cggcctcctc cagccgccgg
     4441 aggtgcggcc ggagcgtgtt gggatgcaga gagaggcggc gcgacatctc ccgcacggac
     4501 accggccggt ccatcagccg caggtgccgg tacaggcgga agcgcgtgtt gtcggcgagc
     4561 gccttgtgga tctcgagggc cttggtgtcc atgcgccccc ctcgggtcgg agcgggcacg
     4621 agccaggata ctatggaggc gtccgcgacc tcgcggagga gccatgatca ccgagcagga
     4681 cgttcgccac gcgctgcacc gcgcgctgga tccgagcatc gagaagcccc tggacgacct
     4741 gggggccgtc gaagccgtgg agaccgaccg cgaccgggtg cgcgtctcga tccgcgtgcc
     4801 gatccccggt gacgacgaga acgtgcgccg ctcggtggag gaggccctgg ccggcctcgg
     4861 cgcccacctc gaggtccgcg tgagcgagat gagccccgag cagcgggccg agatggtcgg
     4921 gcggctccgg ggcgggaaga cccgagagga gaccgtgttc ttctccgagg gccccaccga
     4981 ggtcgtcgcc gtcgcctcgg ggaagggcgg ggtggggaag tcgagcgtca ccgcgaacct
     5041 cgcggcctcg ctcgcggccg agggccagcg cgtggggatc ctcgacgccg atgtctgggg
     5101 gttctcgatc ccccggatga tgggcgtgag cggtcagccg gccggcacga gcgacatgat
     5161 cgtcccgccc cgggcccacg gcgtgaagat catctcgatg ggcttcttcg tctccgacga
     5221 gacgcccgtg atctggcggg gcccgatgct gcacaaggcg atccagcagt tcctgggcga
     5281 cgtctactgg ggggagctcg acgcgctctt cgtggacctc cccccgggca cgggcgacgt
     5341 ctcgatctcg ctggcctcgt tcctgccccg cggcgccatg ctcgtggtga cgacccccca
     5401 ggaggcggcg gcgaaggtgg ccgaacgggc ggggcggatg gcccagcatc ccaacatcga
     5461 cctcgaggtc ctcggcgtgg tggagaacat ggccggcttc gtctgccccc actgcggcga
     5521 gatcaccccg ctgttcggcg aggggggcgg ggcccgcacg gcggaggcgc tggacgttcc
     5581 gctgctcgga tcggtgccga tccaggtctc gctgcgcgag gggtccgacg agggcacgcc
     5641 tgtggtcgtc agcgagccgg actcacccgc cggcgccgcg ttccgcgaga tcgccggcga
     5701 gctggggacg cggatccgca gcccgagggg gaagccgctc agtctccggg agacctgagc
     5761 cgcggctcgg gcgggggcgt gaagctcgtc gtcggtccgg aggcgagcgc cgacgagcta
     5821 tcctccgcct ccgggatcgt cacggccgcc ggaccgggtc ggagtgcgaa ccggacgagc
     5881 accgcgaccg cgaccgcgat caccccgacc gaggcccact ggctccccgt gagccccagc
     5941 ggccacgtct tgtccacccg caggaagtcg gtcagcaggc gccccgcccc gtaccagatg
     6001 ccgaacgcgg cgatcagcac gccctcgcgg cgtggcttcc ggctgagcca gaagaggaac
     6061 gcgaagagcg cccccgcgat cagaatgtcg tagagggccg tctggtggac gccgggcccc
     6121 tgcgccagca ccgcccccgt caggtcgcag gtcccctcgt agagctcggc gccgtccggc
     6181 cggatcacct ggcagtgcgg tccggccagg ctcgcgatcc acgtgtccgg cccggcggcc
     6241 tggtacggac cgggcagcgt cccgctcgtg tatcgccatc cgatgaggaa gtcggtgggc
     6301 ttgcccaggt ggtcgccgat cacgaggtcg ccgatccgcc ccaccgcgat cccgaaggcc
     6361 aggcccaccg cggccgagtc catcacctgt ccgaagcggt agccgtaccg ccgcatcagc
     6421 ggcacggcga agccgatcgc cccgaagatc ccgcccacga gactgatgcc cccgcgccag
     6481 atcgccagca tgtccgtgag cgaatcgaac tcgtccaggt ggccgaggac gtagaagaag
     6541 cgggccccca cgatcgcccc caccagggcc cagaggatga tcgtggagat gtgctcctcg
     6601 cgaaggcccc gcttggggcc ctcgcgcatc atccaccagg cgcccaggag gtaccccacg
     6661 gcgatcccga tcccgtgcgg cgagatggcg agcggcccga ggtcgatgcg gtcgagcacg
     6721 ggccacgaca gggtggcgag cgcgtccatc gacgggagtc tagcggacgt ccgggtcggg
     6781 ggccgtccag acgtcggcca tggccctccc caggagcagg cctccggcga cgtcggacgg
     6841 atagtgcacg ccgaggtaga cgcgggagag accgacggcc ccggccagcg ccacgagcgc
     6901 cgagcgggcg gggcccggga ggtcgagccg tcgggccgcc acgagggcga aggtgaggaa
     6961 gacggcggga tggctggacg gccacgaggt gccgcggggc ttcccgatca ggagcctgcc
     7021 cggcgcggtg gagtcgaagg gccgggtacg ccgataggcc ctcttcagtg cctgccccag
     7081 ggcccacatc gaggccgccg cccccaccgc ccgggctgcg gcgcgacggc tccccgacgt
     7141 cgcgaggacc gctccggccc ccagcgacgc gtagagcgag cccagctcgg tcacctccga
     7201 gagcagccga tcgagcgctg ggtgctcgag gttcgtgttc atctgcttgt agaggcgctc
     7261 gtcgagctcc cgtcctcgga ccgagccggt gatcgccgcc agccccagcg ccgcgccggc
     7321 gagccccagc gctcgtccca ccctccggcg ccgcctcctc atctcccctc caccatctcc
     7381 ttctgggccc ggaggccgcg ccggaggatc agcgaggcgc ccatcaggcg gacggcgcgg
     7441 gccgcggcgc ggcccagcgc gcccccgggg aagcggtact ccacctcgtg ctccacgcgg
     7501 gagcggcggg cgccgatcgg gcggacgaag gtccggacca ccccttcgat gggcccgctg
     7561 agcgccacct ccgagtacgt gggacgctcc agccgcttca ccgaggcgcg gacctccgcc
     7621 gacaccccga gcaggccgag ctcgacccgg tacgagtccc ccgtctggag ctcgccgtcg
     7681 ggggctccaa tgaccgcctt gaggtgacgg ttccagcggg gcaggttccg ggggtcggcg
     7741 atcacgcgcc acacctcctc gggggtcgcg tcgacgacga ccgcgtctcg gatcgtgctc
     7801 atcggccgcc aaggctaccg ctcgcccagc cccgccacca cacgacgcga tcggggcgca
     7861 gccgcaccac cgcgtcgccc tccgtcgccc ccgaacggac ggcgagcgac gcctcgcccg
     7921 agcggaggcg ccccgggacg tacacgcgtc cctcacctcg gagctgcatc cccagcatcc
     7981 gcgaggcccg ccaccgggag aggtggtcga gcacgagcgt cgccggcgcc cgatccgcgg
     8041 cgccggtccg ctccaggaac gaagcgggga cgaccgcgta gtaggccccc tcctcctcgg
     8101 agcgcctcca tcggacgggg aggaccgggg cgtcgtccag ggccagcgcg cctcgtccct
     8161 cgtcaccgag ggcgtcccgc acgtcgccgg ggacgcccgc gtcgggggcg ggtcgggcag
     8221 gcccccggcg gaacgaggtc gcgccgatca gacccccgtc gagcctcccc caggagcgga
     8281 gcacctctcc ctccgagagg tccagcagga ccccgtctcg gagcgcgatc gagaggaaga
     8341 ccctgcccgg cggcgtccag ccgaagggca cacggcgcgc gtcgcgggcg tagccggcga
     8401 agaacctcgc gttcttccgc gagaagccga gcgccgcccg ggtcagcagc tgactccgga
     8461 ggagggagcg gccccaggtc gtggggtcga ggaggtcgta gagatcgacg cggccccgga
     8521 aggacacggc ccggtcgccg acccggacga gccctcctcc gtccggcctc gctcgccaca
     8581 ggcgaacctt ccgggaccga cgcgctgtgg tggcccagag gcggtcctcc gccaggacga
     8641 agaccatagg agtgaggtgc ggtgcgtcgg ggccggccgc cgcggcgtag aggagcacgc
     8701 cgtctcggag cacccggcgg gcctcctcgg ggatcatggc tctgatcctt cccccacggg
     8761 cggacggagg atcatgagac ccgtccacac gacgtggcag gcgacgctcg ccaggatccc
     8821 gcccgtccag agggcgaggg ccgtccacgc ggcgccgccg accacggcgc ccagcacgat
     8881 ggggacgctc ccgctcacga ggttggccag gacgtagagc gcccaggcca gcgcggcgcc
     8941 gggggcctcg ccgagggccc cgctcaggcg tccctggatc aggccccgcc acagcgactc
     9001 ctcggccgga gccacgacga ggcacgcgat cgcgagaacc gcgccgagcg acagcccccg
     9061 gcgccgcgcg tagagctcgt gggcctgccg ccggaggatc cgccatccgc gggcgaggaa
     9121 gaggaaggtc gcggtggcag cgtagaaggc gagccccgcg ccgaggccga tcagggcctc
     9181 gaggagcccg ccccccgcct ccaccccgcc ggacagcagc gcgagcgcgc ccaggggacc
     9241 caccgtccac gccatcgcgg cccagatcga gacccctccg atccgcaccg cggcccaggc
     9301 ccccgccaag gcgagcggcc cgatccacgc caccgccacc gcgacggcgt ccatctcagc
     9361 ccaggaaccg gaccttcgcg gcgagctcgg cggtgaaggc cgtgatcacg gcgaggtaga
     9421 agaagcccgt cgcggcctgg acggcccggg ggcgcgtctc gcgcagcgcg gcccggatca
     9481 ggaacgcgat gagggcggtg atcgccacca tgccgaggac gatcgccagg tggaaggcgg
     9541 caccccccac ggtgaggaac aggccgaacc cctccgtcgg cgtcacgtcc ccggccacgg
     9601 ccgcgatcat cacggccacg gcctcgacca ggacggcccc gatcagcacg acggccatcc
     9661 gctggatgtg atcccgggcc agactccggt ccacgaggta ccagtgcccc agcagcaggc
     9721 cgacggtgac ggcgcccatg aaggcggctc cggccaagag cccgacgaga gccatcgcca
     9781 tccccgcctc cgccaccgac gccatcgcca caagcagcgc cacccccacc gggatcgaga
     9841 ccaggcccag gacgcgtccc gcggccatgc ggcgcaggat gagcgcgatc agggcgaggg
     9901 ccgagagcgc cgagagggcg agagccagga cgaccacgag gcgcgctccc ccactccccg
     9961 cctcggtggc gccggcgttc gcggacaggg ccgccagcag agcgcaggcc agcacggtcg
    10021 ccgtcgtgag gatgaagaag ccgcgcttca cgttccccca gagccccgtg agccagagaa
    10081 gggcggcgct gccggccgcg gtctcggcca gcacgagcgc gatcacgccg gatgcccctt
    10141 ccatgcgcgg gatagcctac aagggatgct gcagcccgga accagcgcca cggtggccga
    10201 gaccgcggcc gaggagcaga ccgccgagcg gatcggctcg ggcgacgtcc ccgtctacgc
    10261 caccccgatg ctgctggccc tcgcagagcg ggcagcggtg gccgcgatca agactgcgct
    10321 cgagcccggg atgacctcgg tcgggacgag ggtcgagctc gaccacctgg ctccgacgcc
    10381 ggccgggtcg agagtggagg ccacggccac gctcaaggag gtcgacgggc gcaggctcgt
    10441 cttctcgttc gaggcgcgcg acccgggcgg cgtcgtggcg cgcggcacgc acgtcagggt
    10501 gctggtgcgg cgagggccgt tcgtggaggg ggcgcggtcg cgggtcgtgt gaac
//
LOCUS       DASUTW010000202         5781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1098909, whole genome shotgun sequence.
ACCESSION   DASUTW010000202 DASUTW010000000
VERSION     DASUTW010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098909"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VF058_07015"
     CDS             complement(<1..612)
                     /locus_tag="VF058_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX6208093.1"
                     /translation="MRRVVILGSTGSIGTQALDVIRRNPERFEVVGIAAGSSQELLVG
                     QVREFMPPLVAVSDPDAVAELRAGLEGLRTVEILTGPDAPEQMARDLDADLVLNAMVG
                     RIGLEPTLAALQTGKTVALANKESLVVGGELVMDLVKGEPERLVPVDSEHSALSQCLR
                     GERREDLRRVLITASGGPFRGWTRDELEKASVKEALAHPTWKMG"
     gene            complement(678..2549)
                     /locus_tag="VF058_07020"
     CDS             complement(678..2549)
                     /locus_tag="VF058_07020"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX6208094.1"
                     /translation="MSEDERREDVDDLFGDLDDFFAPIDDVEWPEGAEREEAEGEGAK
                     AEKGEAEGEAEAAEPNEWAPAIEIPDEEELVGRGELGEPDAEAAEAAEAEDPSDAPPP
                     EQPAPEEPEPSLTVDDFFDDEEAEAPEEEEEEEEEIQPTAEMSGEDWARLRSALGEDE
                     EPEEPVEPRGEEAAEPRELTIDDLADGPDQYEELRGEEPGDEEAVLAEEAIFEPAEGQ
                     DPEGVEVAADEPATEEEVEAAAAHFASGIEPEEVERDLLSDLDEEAPARMAAEPEPVE
                     EPEAEAPEEAPAEDVFEDEEGRKVMRVTPRPTAPLEGEEEPLPLGGTGAPTWQEPAAY
                     AADEEVPAEAGAPPPGRNLGAAVASGVILAAAAVALLAIDKGPFVWLAVAVVLLGQGE
                     LYAVLRTRGFQPATAIGLLAGLFVMVGAYTRGGSGQGEAAMLFGLALAALFSPLWFMA
                     AAPSARRGLVANVGVTLLGVLYAPFLASFALLLLAIPGNDGRNLLLTVVGLTVLYDVC
                     AYGVGTFWGDRPLAPTISPRKSWEGAIGATFIVVLVALAIVPLFEPFDHPGPAVGLAI
                     VIAIAAPLGDLAESAIKRDLGVKDMSSLLPGHGGVLDRLDAILFTAPAAYYFLRLVL"
     gene            complement(2549..3109)
                     /gene="frr"
                     /locus_tag="VF058_07025"
     CDS             complement(2549..3109)
                     /gene="frr"
                     /locus_tag="VF058_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX6208095.1"
                     /translation="MAEADVLKEADHKMKRAAQVAQDEFAGVRTGRATPAILNRVMVD
                     YYGTPTSLNQLASMAVPEPRVLTIQPFDPNSVSAIEKAIMQSDLGLTPSNDGHLIRLV
                     FPQLTEERRKELIRVVRERAEEGRISIRNVRRHAKDELERMKKDGEISEDDLVRAEKE
                     LQQMTDKHVAEVDELLQRKEQELMEV"
     gene            complement(3114..3863)
                     /gene="pyrH"
                     /locus_tag="VF058_07030"
     CDS             complement(3114..3863)
                     /gene="pyrH"
                     /locus_tag="VF058_07030"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017614701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX6208096.1"
                     /translation="MTAESGTAATPERPLYGRVLLKLSGDAFAPPDREFGISEQSVEL
                     LSAQLADVVSVGTQTAVVVGGGNIFRGRDAPNMDRARADYMGMLATVMNALALQDALE
                     RAGVPTRVQTAIQMAQVAEPYIPRRAIRHLEKGRIVIFAAGIGQPYFSTDTTAAQRAL
                     EIHAEAILKGTQVDGVYDKDPRKNADAVRFEQLEYIEVLQRGLQVMDATAISLCMENG
                     LPIIVFDFRVEGNIKRALTGERIGTLVHAGG"
     gene            complement(3874..4479)
                     /gene="tsf"
                     /locus_tag="VF058_07035"
     CDS             complement(3874..4479)
                     /gene="tsf"
                     /locus_tag="VF058_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX6208097.1"
                     /translation="MEITTEMVRELREATGAGMMDCKRALEEAGGDMARATELLRARG
                     LADAAKRADRVAAEGLVEAYIHMRGSLGVLVELNCETDFVANTDDFRSLARDVAMQIA
                     AIAPRWIAREEVPGDVVDGERKVYEEQARQEGKPDDVIPRIVEGKLEAFYGDHVLLDQ
                     PWVRDDSVTIGELISQASAKLGEKIEVGRFARFKVGEAARS"
     gene            complement(4509..5558)
                     /gene="rpsB"
                     /locus_tag="VF058_07040"
     CDS             complement(4509..5558)
                     /gene="rpsB"
                     /locus_tag="VF058_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017576203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX6208098.1"
                     /translation="MPVVTRRQLLEAGIHFGHQTRRWNPKMRRYIFGERSGIYIVDLE
                     KTLEGLERAYEFVRDLARRDGTVLFVGTKKQAQDVVAEQAGRVGMPFVNTRWLGGMLT
                     NFRTIFARLQRLTELRRMEESGAFEFLPKKEVLKLRHEKEKLERNMGGIQDMKRVPDA
                     VFVIDTLKEHIAVTESNKLGVPLIAIVDTNCDPDQVDYVIPGNDDAIRSVSLVASVIA
                     DAIEEGKQLAAKDFAERAMGEEEASPEPPAAEREEVSAPKPPAAERGEVAAPEPEPAP
                     AADTSVSESSPAADAERTGEVAPAEPEREPARPGGTSSQPGAGDRAAAGDPEPEASSE
                     AEQPAGGEDEGRGES"
BASE COUNT          735 a         2219 c         1907 g          920 t
ORIGIN      
        1 gcccatcttc caggtcgggt gggccagggc ctccttcacc gaggccttct ccagctcgtc
       61 ccgggtccag ccccggaagg gcccgcccga ggccgtgatc aggacgcgac gaaggtcctc
      121 gcgtcgctcg ccccgcaggc actgggagag cgccgagtgt tccgagtcca cggggacgag
      181 ccgctcgggc tcgcccttca ccagatccat gacgagctcg cccccgacga cgaggctctc
      241 cttgttggcg agcgccacgg tcttcccggt ttggagggcg gccagcgtcg gctccaggcc
      301 gatccgaccc accatcgcgt tgaggaccag gtcggcgtcg aggtcccgag ccatctgctc
      361 cggcgcgtcg ggaccggtca ggatctcgac cgtgcgcagg ccctccagcc ccgcccgcag
      421 ctccgccacc gcgtccggat cgctcacagc caccagcggg ggcatgaact ctcggacctg
      481 acccaccagg agctcttggc tggagccggc cgcgatgccg acgacctcga atcgctccgg
      541 attgcgccgg atcacgtcca gcgcctgcgt cccgatcgag cccgtcgaac cgaggatgac
      601 gactcgccgc ataccgctcc cagggtaccc gctcgcgttg gcaattacat cgaggtcgtc
      661 cgtatccccc ggaggggcta caggacgagc ctcaggaagt agtacgccgc cggggcagtg
      721 aagaggatgg catcgagtcg atccagcacg cccccgtggc ccgggagcag cgagctcatg
      781 tccttgacgc cgaggtcgcg cttgatcgcc gactcggcca ggtcgccgag cggcgcggcg
      841 atcgcgatca cgatcgccag gcccaccgcc gggcccggat ggtcgaaggg ctcgaagagc
      901 ggcacgatcg ccagggccac cagcaccacg atgaacgtgg cgccgatcgc cccctcccac
      961 gacttcctcg ggctgatcgt cggcgccagc ggccggtcgc cccagaaggt tcccaccccg
     1021 tacgcgcaca cgtcgtacag caccgtcagt cccaccaccg tgaggagcag gttccggccg
     1081 tcgttgccgg ggatcgccag cagcagcagc gcgaacgacg ccaggaaggg ggcgtagagc
     1141 acgccgagga gggtcacgcc cacgttcgcc acgagccccc ggcgcgcgga cggcgccgcc
     1201 gccatgaacc agagcggcga gaacagggcg gcgagggcca ggccgaacag catcgcggcc
     1261 tctccctggc ccgagccgcc cctcgtgtag gcgcccacca tcacgaacag ccccgcaagg
     1321 aggccgatcg cagtggccgg ctggaagcca cgggtccgga gcacggcgta cagctccccc
     1381 tgtcccagca gcacgaccgc caccgccagc cagacgaacg gccccttgtc gatcgccagc
     1441 agcgcgacgg cggccgcggc caggatcacg ccggacgcga cggccgcccc caggttccgc
     1501 cccggcggcg gcgcccccgc ctccgccggg acctcctcgt ccgcggcgta cgcagctggc
     1561 tcctgccagg tgggcgcccc cgtgccgccc agcgggagag gctcctcctc cccttccagc
     1621 ggagcggtcg ggcgcggggt cacccgcatc accttccgcc cctcctcgtc ctcgaagacg
     1681 tcctcggcgg gggcctcctc cggagcctcg gcctcgggct cctcaaccgg ctcgggctcg
     1741 gccgccatcc gggccggggc ctcctcgtcc aggtcggaga gcagatcgcg ctcgacctcc
     1801 tccggctcga tgccggatgc gaagtgcgcg gcggcggcct ccacctcctc ctccgtggcg
     1861 ggctcgtcgg ccgcgacctc cacgccctcc ggatcctgcc cttcggccgg ctcgaagatc
     1921 gcctcctcgg ccaggaccgc ttcctcgtcc ccgggctcct cgcctcgcag ctcctcgtac
     1981 tggtcgggcc cgtcggccag gtcgtcgatc gtcagctcgc gcggctcggc cgcctcctcc
     2041 cctcgaggct cgacgggctc ctcgggctcc tcgtcctccc ccagggcaga gcggagccgg
     2101 gcccagtcct ccccggacat ctcggcggtg ggctggatct cctcctcttc ctcctcctcc
     2161 tcctcgggag cctcggcctc ttcgtcgtcg aagaagtcgt ccacggtcag ggagggctcg
     2221 ggctcctcgg gggccggctg ctcgggcggg ggagcgtcgg aggggtcctc ggcttcggcg
     2281 gcctcggcgg cttctgcgtc gggctccccg agctcgcccc ggcccacgag ctcctcctcg
     2341 tccgggatct cgatcgcggg ggcccactcg ttcggctccg ccgcctcggc ctcgccctcg
     2401 gcttcgccct tctccgcctt ggccccttcg ccctcggcct cctcgcgctc ggcaccctcg
     2461 ggccactcca cgtcgtcgat cggcgcgaag aagtcgtcga ggtcgccgaa caggtcgtcg
     2521 acgtcctctc gtctctcgtc ctcgctcatc agacctccat gagctcctgc tccttccgct
     2581 gcaggagctc gtccacctcg gcgacgtgct tgtcggtcat ctgctggagc tccttctcgg
     2641 cgcggacgag gtcgtcctcg gagatctcgc cgtccttctt catcctctcc agctcgtcct
     2701 tggcgtggcg acggacgttt cggatcgaga tgcgcccctc ctcggcccgc tcgcgcacga
     2761 cccggatcag ctccttccgc cgctcctcgg tgagctgggg gaagacgagc cggatcaggt
     2821 ggccgtcgtt ggacggggtg agccccagat cgctctgcat gatcgccttc tcgatcgcgg
     2881 agaccgagtt gggatcgaac ggctggatcg tgaggacccg cggctcgggc acggccatcg
     2941 aggcgagctg gttcagcgag gtgggcgtgc cgtagtagtc gaccatcacg cggttgagga
     3001 tcgccggcgt ggcccgaccg gtgcgcaccc ccgcgaactc gtcctgggcc acctgcgccg
     3061 cccgcttcat cttgtggtcg gcttccttaa ggacgtccgc ttccgccatc cgtctatcct
     3121 cccgcgtgca ccagcgtccc gatccgctcg ccggtcaggg cgcgcttgat gttcccctcc
     3181 accctgaagt cgaagacgat gatgggcaag ccgttctcca tgcacaagga gatggccgtg
     3241 gcgtccatca cctggagccc ccgttggagg acctcgatgt actcgagctg ctcgaagcgc
     3301 acggcgtcgg cgttcttgcg cgggtccttg tcgtagaccc cgtcgacctg cgtccccttg
     3361 aggatcgcct cggcatggat ctcgagggcc cgctgggccg cggtcgtgtc ggtggagaag
     3421 tacggctggc cgatccccgc ggcgaagatc acgatccgcc ccttctccag gtgacggatc
     3481 gcgcgccggg ggatgtacgg ctcggcgacc tgcgccatct ggatcgcggt ctggacccga
     3541 gtggggacgc ccgcgcgctc cagggcgtcc tggagggcca gggcgttcat caccgtggcc
     3601 agcatcccca tgtagtcggc gcgtgcccgg tccatgttcg gcgcgtcccg gccgcggaag
     3661 atgttgccgc cgcccaccac cacggcggtc tgggtgccca ccgagacgac gtccgccagc
     3721 tgggcggaca ggagctcgac cgactgctcg gagatgccga actcgcggtc gggaggggcg
     3781 aaggcgtcac cggagagctt caggagcacc cgcccgtaca gcggccgctc gggcgtcgcc
     3841 gccgtccccg actcggccgt caatggccct ccctcagctc cgggccgcct cgcccacctt
     3901 gaagcgtgcg aaccggccga cctcgatctt ctctccgagc ttcgcgctcg cctgggagat
     3961 cagctctccg atcgtgaccg agtcgtcgcg cacccacggc tggtcgagga gcacgtggtc
     4021 tccatagaac gcctcaagct tcccctccac gatgcgaggg atcacgtcgt cgggcttgcc
     4081 ctcctggcgt gcctgctcct cgtagacctt ccgctcgccg tccaccacgt cgcccggcac
     4141 ctcctcgcgc gcgatccagc gcggggcgat cgccgcgatc tgcatggcga cgtcgcgagc
     4201 cagcgaccgg aagtcgtcgg tgttggccac gaagtcggtc tcgcagttga gctccacgag
     4261 cacgccgagg ctgcctcgca tgtggatgta ggcctcgacg agcccctcgg ccgccacgcg
     4321 atcggcgcgc ttggccgcgt cggcaaggcc ccgggcccgg agcagctcgg tggcgcgcgc
     4381 catgtcgccc ccagcctcct ccagggcgcg cttgcagtcc atcatgccgg ccccggtggc
     4441 ctcccggagc tccctcacca tctcggtcgt gatctccatc ggtcctcgcg attctcccat
     4501 agtgtcgatc aggattcccc tcgcccctcg tcctcccctc cggccggctg ctcggcctcg
     4561 gaggaggcct ccggctcggg atcccccgcc gccgctcgat cccccgcccc gggctgggag
     4621 gaggtccctc ccggcctggc cggctcgcgc tcgggctcgg cgggggcgac ctcgccggtc
     4681 cgctcggcgt cggccgcggg gctcgactcc gagaccgagg tgtcggcggc gggcgccggc
     4741 tccggctcgg gggccgcgac ctcccctcgt tcggcggcag gcggcttggg ggccgagacc
     4801 tcctcccgct cggcggcggg cggctcggga gaggcctcct cctcccccat ggcccgctcc
     4861 gcgaagtcct tcgccgcgag ctgcttcccc tcctcgatcg cgtcggcgat cacactcgcc
     4921 acaagcgaga cggaccggat cgcgtcgtcg ttcccgggga tcacgtagtc gacctggtcg
     4981 ggatcgcagt tcgtgtccac gatcgcgatc agggggacgc cgagcttgtt cgactccgtg
     5041 accgcgatgt gctccttcag cgtgtcgatc acgaacacgg cgtcggggac ccgcttcatg
     5101 tcctggatcc cacccatgtt gcgctcgagc ttctccttct cgtggcggag cttcaggacc
     5161 tccttcttgg ggaggaactc gaaggccccg gactcctcca tgcgccggag ctcggtcagc
     5221 cgctggagcc gcgcgaagat cgtgcggaag ttcgtgagca tcccgccgag ccagcgcgtg
     5281 ttgacgaacg gcatgccgac ccgccccgcc tgctccgcga cgacgtcctg ggcctgcttc
     5341 ttcgtcccga cgaagaggac cgtgccgtcg cggcgggcga ggtcgcggac gaactcgtag
     5401 gcgcgctcga ggccctccag cgtcttctcg aggtcgacga tgtagatgcc gctccgctcc
     5461 ccgaagatgt agcgccgcat cttcgggttc cacctgcggg tctggtggcc gaagtggatg
     5521 ccggcctcga gcagctgccg gcgcgtgacg acaggcatgg tgcttcctcc ttccggttcg
     5581 gtcctccgcc cggctccacc ccgctccaac ccgcttcgcg ggaccgggga gcgggtcccc
     5641 gggcgtgtgt cgtaccccct gaggggccgg aggcgatggt agcagaacgg ctggtcaggc
     5701 ggtccgctcg cgccggcgac gcggggcggg acccgtcggc acgacgccgc ggacgacgat
     5761 ccagcggtag gtcagcgcgc c
//
LOCUS       DASUTW010000203         3486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1229053, whole genome shotgun sequence.
ACCESSION   DASUTW010000203 DASUTW010000000
VERSION     DASUTW010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229053"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..527)
                     /locus_tag="VF058_07045"
     CDS             complement(75..527)
                     /locus_tag="VF058_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208099.1"
                     /translation="MATDPLRPGDEVPGIPDVDLSRPALVAFYKVTCPTCQVAAPPME
                     RLHRAADGGFVAVVQDGSDRAAAFATEHGVSFDSIPDEDPYPISRAWGVRVVPTLFAT
                     AEGRVEDLVESWDREGWARVAARFGELRGILVPTPSEEGDGLPSFRPG"
     gene            complement(530..2077)
                     /locus_tag="VF058_07050"
     CDS             complement(530..2077)
                     /locus_tag="VF058_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX6208100.1"
                     /translation="MQGRHAPEGSLTRRLGFTGLVVLLLAATAAVASWRFGLFSEASP
                     EPPTRAGGPIPAPTTPAAEVSPEPSPSPPFTPEPINTGVEGLTTFRGNATRTYHGRGP
                     VPEDPEILWRYPTTGGLCGESSDAGGTRTWCGTGWTGQPNVVEWEGRTQIRFGAYDYA
                     VHILNGRNGRPIKEPFRTGDLIKGTVTSDPDGYPLLYVGSRDNQLRILALDRGRTMEE
                     LWSLPADSAPDPTWNDDWDGSPLVIDDHLLEGGENSWFYVVKLNRGYDDQGSVTVNPR
                     VRVMVPGYDDELIAALGDEQVSIESSVAFHDGVAYFANSGGLVQGWDVSDVLSGGDRH
                     RRVFRFWTGDDTDASVVIDEEGYLYVASELERLNGRSREVGQLMKLDPRKPKDPLVWS
                     VPVPGEGGAGGLWATPAVHGRLVIAPTNTGRILAVNRNTGRIRWELRLPPPTWTSPVI
                     VDDVLILGDCAGVLHAYDLSRPRREPPELWSVQLEGCIESTPAVWDGMVFVGARGGAV
                     YGIGDRG"
     gene            2190..3455
                     /locus_tag="VF058_07055"
     CDS             2190..3455
                     /locus_tag="VF058_07055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6208101.1"
                     /translation="MTDTRTPARGVPGVGVDLLRSERSMIAVRWVATAFGLVQILAYE
                     DAPYPEEIPVFALGLAAMAVLAAGNGALWIASRREVGARALALAGLALDVLVAEAIVW
                     LWAFDPGSALWAILYVLPLEGAIKFQLRGALGTWAAVTALYVAREFWGYATFEGFNLQ
                     WNSITFRMGLGLLIALVGGLMARNLTRERALAEDRLTRLRRIDRLRAGLVSTLAHDVR
                     NPIAAIRGTITILLSPRVGRLSKETRLDLLRSADRQAERLERLAIDLLDLARLERGRL
                     ELTFENVSMAPLVRRALAYVEGAHRIEVEVPDDVVLRADPGRVEQILVNLVSNALRYG
                     REPFELRVSSPVHEVVVQVTDRGEGIPPDQQPTLFDPFRSEREGGSVGFGLAIVKALA
                     EAHGGSAAYRDRDGGGACFVVRLPVAGPV"
BASE COUNT          491 a         1234 c         1251 g          510 t
ORIGIN      
        1 cgagagaccc gccccgaact ccacgtcccc caccctatcg gctccgctca gaccgagcgc
       61 gcgctcctgg acgctcaccc ggggcggaag gaaggaagcc cgtcaccctc ctcggagggc
      121 gtaggcacca ggatcccgcg gagctccccg aagcgggccg cgacccgggc ccacccctct
      181 cgatcccagg actcgacgag gtcctccacg cgcccctccg ccgtggcgaa cagcgtgggg
      241 acgacccgca cgccccaggc acgcgagatc ggataggggt cctcgtccgg gatcgagtcg
      301 aacgagaccc cgtgctccgt cgcgaacgcg gcggcgcggt ctgagccgtc ctgcacgacg
      361 gccacgaagc ccccgtccgc ggctcggtgg agccgctcca tcggcggggc cgccacctgg
      421 cacgtggggc aggtgacctt gtagaaggcg acgagcgcgg gacgcgagag atcgacatcg
      481 gggatcccgg ggacctcgtc gcccggacgg agcggatccg tcgccaccgc tacccccgat
      541 ccccgatccc gtagaccgcg ccgccgcggg cccccacgaa gaccatgccg tcccacacgg
      601 ctggggtgga ctcgatgcac ccctcgagct gcacgctcca gagctcgggg ggctcgcgcc
      661 tggggcggga gaggtcgtac gcgtggagca ctcccgcgca gtccccgagg atgagcacgt
      721 cgtcgacgat caccggcgag gtccacgtgg gcggcgggag ccgcagctcc cagcggatgc
      781 gtcccgtgtt gcggttcaca gccaggatgc gtcccgtgtt ggtgggggcg atcacgagcc
      841 gcccgtgcac ggcaggcgtg gcccagaggc ctcccgcgcc tccctctccc gggacgggaa
      901 ccgaccatac gagcgggtcc ttcggcttgc ggggatcgag cttcatgagc tgccccacct
      961 ctctggagcg gccgttcaat cgctcgagct cggaggccac gtacaggtag ccctcctcgt
     1021 cgatcacgac cgaggcgtcg gtgtcgtccc ccgtccagaa acggaacacg cggcggtgcc
     1081 gatcgccccc cgagaggacg tcggagacgt cccagccctg caccaacccc ccggagttgg
     1141 cgaagtacgc cacgccgtcg tggaaggcga ccgagctctc gatcgagacc tgctcgtcgc
     1201 ccagggccgc gatcagctcg tcgtcgtagc ccgggaccat cacgcggacc cgggggttca
     1261 cggtgacgct cccctggtcg tcgtagcccc ggttcagctt cacgacgtag aaccaggagt
     1321 tctcccctcc ctccaggagg tggtcgtcga tcacgagcgg ggagccgtcc cagtcgtcgt
     1381 tccacgtggg atctggggcc gagtcggccg ggaggctcca gagctcctcc atcgtgcgcc
     1441 ctcgatccag cgcgaggatc cggagctggt tgtcgcggga gcccacgtag agcagcgggt
     1501 acccgtcggg gtcgctggtg accgtcccct tgatcaggtc cccggtcctg aagggctcct
     1561 tgatcgggcg gccgttcctg ccgttcagga tgtggacggc gtagtcgtaa gctccgaacc
     1621 ggatctgggt gcggccctcc cactccacga cgttgggctg ccccgtccac ccggtgccgc
     1681 accaggtcct cgtcccgccc gcgtccgagg actccccgca gaggccgcct gtcgtcggat
     1741 acctccacag gatctcggga tcctcgggga cgggccctcg gccgtggtag gtccgggtgg
     1801 cgttgccccg gaaggtcgtc agcccctcga caccggtgtt gatgggctcc ggggtgaagg
     1861 gcgggctggg gctcggctcg ggggagacct ccgccgcggg agtggtcggg gccgggatgg
     1921 ggccgccggc ccgggtggga ggctccggcg aggcctccga gaagagcccg aaccgccacg
     1981 aggcgaccgc cgcggtggcg gcaagcagga ggacgacgag tccggtgaac ccgagacggc
     2041 gggtgagcga accctcagga gcatgcctcc cctgcatcgg agccatgcta cgggaaggcc
     2101 ccccgccccg gaagaggaca tggactgccg gtagccgttc gagcgacact aggtgccgcc
     2161 cggatggcac gtggagcgac gatgacccga tgaccgatac gaggaccccc gcccgcggcg
     2221 tccccggcgt gggcgtggac ctgcttcgat cggaacgctc catgatcgcg gtgcgctggg
     2281 tggcgaccgc gttcggactc gtccagatcc tggcctacga ggacgcgccc taccccgagg
     2341 agatccccgt cttcgcgctc ggcctggcgg cgatggcggt cctggcggcg ggcaacgggg
     2401 ctctctggat cgcctcccgc cgcgaggtgg gcgcccgcgc gctggcgctg gcggggctgg
     2461 cgctggacgt cctcgtggcc gaggcgatcg tctggctctg ggcgttcgac cccgggtcgg
     2521 ccctgtgggc gatcctctac gttctcccgc tggagggcgc catcaagttc cagctccggg
     2581 gcgccctcgg gacctgggcg gccgtcacgg ccctctacgt ggctcgcgag ttctggggat
     2641 acgcgacctt cgagggcttc aacctccagt ggaacagcat caccttccgg atgggcctcg
     2701 gcctcctgat cgccctcgta ggaggtctga tggcacggaa cctcacgcgc gagcgggccc
     2761 tggccgagga tcgcctgacc cggcttcgca ggatcgaccg gctccgggcc ggcctcgtct
     2821 ccacgctcgc ccacgacgtc cggaacccga tcgcagcgat acgggggacg atcacgatcc
     2881 tcctctctcc ccgggtggga cgcctgtcga aggagacccg cctggacctc ctgaggagtg
     2941 ccgaccggca ggccgagagg ctggagcggc tcgcgatcga cctcctcgac ctggcccgcc
     3001 tggagcgggg ccggctggag ctgaccttcg agaacgtgtc gatggcgccg ctggtccgtc
     3061 gtgctctcgc ctacgtggag ggggcgcatc ggatcgaggt cgaggtcccg gacgacgtgg
     3121 tcctgcgggc cgatcccggg agggtggagc agatcctcgt caacctggtg agcaacgccc
     3181 ttcgttacgg acgggagccg ttcgagctgc gtgtctcgtc cccggtccac gaggtcgttg
     3241 tccaggtgac ggacagggga gaggggatcc cgccggacca gcagccgacg ctgttcgatc
     3301 ccttccggtc ggagcgcgag ggtggatccg tgggcttcgg cctggccatc gtgaaggccc
     3361 tggccgaggc ccacggcggt tcggcggcgt atcgggaccg cgatggagga ggggcctgct
     3421 tcgtggtgcg gctgccggtg gccgggccgg tctaggacga gacctcgagc atgcggtcga
     3481 tcgcgc
//
LOCUS       DASUTW010000204         6664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_582287, whole genome shotgun sequence.
ACCESSION   DASUTW010000204 DASUTW010000000
VERSION     DASUTW010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6664
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582287"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..845
                     /locus_tag="VF058_07060"
     CDS             204..845
                     /locus_tag="VF058_07060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX6208102.1"
                     /translation="MDIVNPEVERYLRELLDEDHPVAAEMEDRAEEHDFPIVGRVVGQ
                     TMEVLARSIGARRVFEMGSGYGYSAYWFSRAVGPEGEVHMTEGDAENQRLALDYLERA
                     GFADRVRSHVGDALELIDDTPGEFDVVFCDIDKHGYPEAWRKARERIRVGGLYLCDNV
                     LWSGRVAQDDPEDRRPEWTTAIREHNEAVARDERYRSTIVPTRDGVTVALRVA"
     gene            complement(903..1430)
                     /locus_tag="VF058_07065"
     CDS             complement(903..1430)
                     /locus_tag="VF058_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004001723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX6208103.1"
                     /translation="MDIGLLLLRLVLGGTFVAHGTQKLFGWLGGHGLDDTGRFYEEQL
                     GYPRGRLAAALAGLAETGGGLLLAFGLLTPLGAAATVGVMLNATLTVHLRNGFFNQDG
                     GFEYPLVNAAAAAALAFVGPGSISLDAALGLGLSGLAWGFVALLLGLIVGTAVYWSRE
                     MEWEEPGEEEDRRAA"
     gene            complement(1484..3913)
                     /gene="leuS"
                     /locus_tag="VF058_07070"
     CDS             complement(1484..3913)
                     /gene="leuS"
                     /locus_tag="VF058_07070"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX6208104.1"
                     /translation="MNEYRPEEIEPRWVAAWEDERLYEAGGEGDPRPRFYALDMFPYP
                     SGDIHMGHAEAFAIGDVVARFHRMRGLNVLHPIGWDAFGLPAENAAIQRGIHPKTWTY
                     ENIDKQAASFKRYGVSFDWTRRLNTCDPEYYRWTQWLFLRLHERGLAYRKLAPANWCP
                     KDQTVLANEQVVNGACERCGTPVVRKDLTQWFFRITEYAEALLDDMELLEDWSDRVLT
                     MQRNWIGRSEGAEVEFEIAETGDRVTVFTTRPDTLWGVTFMVFAPEHELVPALAERGG
                     TSAEAAELLEWVRSTPLASRDLGESREGIGLGVHAVNPVNGEKVPIFVAPYVLMEYGT
                     GVVMGVPGHDQRDFEFAREHGLEVRVVIQPEGEELEAEEMTEAYPHEGVMVRSGAFDG
                     TPSPASIGRVTAWLEETGRGRPAVTYRLRDWLISRQRYWGAPIPIIHCPECGEVPVPE
                     EDLPVLLPEDVDFAPTGESPLAKHEGFVNVSCPRCGGAARRETDTMDTFVDSSWYFFR
                     YCSPHEDDRPFDPEAVERWMPVGQYTGGIEHAILHLLYSRFLTKVLHDMGMVSFREPF
                     PRLLNQGMVIMQGAKMSKSRGNLVEPLPLIEKWGVDTLRVTMLFAGPVEDDVDWATVS
                     PAGVHSWLGRVWRVGLAVADQEGEDPEDLRRLTHRTIREVAALYERFRTNVAVARLME
                     LTNEIRRVLDEGGSARRAAEALVLMLAPMAPFITEEIWRGTWGRTESVHVQDFPSFDP
                     DLAREETVTLVVQVDGKVRDTIDVPADVDEEEAVELARASEKVRRALGGREVSRVIAR
                     PPRLVNLVTAV"
     gene            4020..5219
                     /locus_tag="VF058_07075"
     CDS             4020..5219
                     /locus_tag="VF058_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208105.1"
                     /translation="MGKDISADESKSKRASEPEAIPRGNGDRAVPAPAPEPVEAPRER
                     RRPLPVLANKVGHFLAAFFLLVLSIELMKGGAEAIAPRLQGTFPVDNMVSTMGLGWLG
                     AYLVLSGSPVAAASLALFTAGATTQAQTFTMLSGSRLGASFIVLLVGFLYAMRQRNRH
                     RRRESIGMGVLALGMTAVVYVPGMFLGYGILKSGLLSGVTWRSSAGINGIVDAVWGPI
                     TRFASELLPGPLLFPLGLAVILASFKLLDFVLPALDGEKHAGERGHWMKHPWAMFGLG
                     MLAALLTLSVSVALTILVPLASKGYIERREAIPYIAGANITTLADTLFAAMLLGNPIA
                     VQIVLAQAIAVAIVTVLLLAFLYRPLQTMIMALDEWIVRTTPRLVAFVAILFVMPIGL
                     LFSGRML"
     gene            complement(5224..6546)
                     /locus_tag="VF058_07080"
     CDS             complement(5224..6546)
                     /locus_tag="VF058_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208106.1"
                     /translation="MPADITPIRRSTVLAAALLALTGLLATPSARAAPQTPRFGPLIE
                     GHAAYEGQSRCAPKEKPGVTAFRKLLVSTYGETWMNSSRACSVGGKSEHKEGRALDWA
                     MNASRPKDRRKVKDLFAWMFADDARGNANAMARRLGLMYVIWNRRWWTSWEGTWKRYC
                     VQKPRGCKTPSGSFTHPHDDHVHFSFSWQGARKRTSFFTPERSFITGMAASSDGGYWL
                     AGGDGSVRAYAAPFHGSKAGRFPPAPIVEMASRPDDDGYWLVLRSGRVIAFGEAELHG
                     RVTRKKAVIVGFAATPTGQGYWLLTKQGKVAAFGDAGRFGRVEKGAAAVSGIVPTPSG
                     HGYWVYAAGGEVFAFGDAEHRGDLADASLAAPVVGGAVSGPDGYWLVTRKGNVHAFGS
                     AQLHGQAAKKGLGPVAAIAGSPSGSGYWVVGRTGRVAAFGDAKLRGSL"
BASE COUNT          978 a         2504 c         2208 g          974 t
ORIGIN      
        1 ccacgagcag cgccagcgcg acgaccccgt acaccgcgag ctccctcgcg gtcagcccct
       61 ccagccagtc gcggaacccg tccatggaag cgcggcgtcc ggccgcgtct cgggaggggg
      121 aggcccgcat cgtaccggcc gccgccgaca cgaacgcccg ggctcaccta tcctgtgccg
      181 gtcccgccga gaggagcgcc gagatggaca tcgtgaaccc cgaggtggag cggtacctcc
      241 gcgagctgct ggacgaggac caccccgtgg ccgccgagat ggaggaccga gccgaggagc
      301 acgatttccc gatcgtgggg cgcgtggtgg gccagaccat ggaggtcctg gcccgctcga
      361 tcggcgcccg ccgcgtcttc gagatgggct ccggctacgg ctattccgcc tactggttct
      421 cccgggcggt gggccccgag ggcgaggtcc acatgaccga gggggacgcc gagaaccagc
      481 ggctggccct cgactacctc gaacgggccg gcttcgccga tcgcgtccgc tcccacgtgg
      541 gcgacgccct cgagctgatc gacgacaccc cgggcgagtt cgacgtcgtc ttctgcgaca
      601 tcgacaagca cggatatccc gaggcctggc gcaaggctcg cgagcggatc cgcgtgggcg
      661 ggctgtacct ctgcgacaac gtactctggt cgggtcgggt ggcccaagac gaccccgagg
      721 accggcgtcc cgagtggacc accgcgatcc gcgagcacaa cgaggccgtt gcccgcgacg
      781 agcgctaccg ctcgacgatc gtccccaccc gggacggcgt gacggtcgcc ctccgagtgg
      841 cctgaatcac cgcttgcgct tggccggcgt ctccacgcac tcggcgggag ggtcgcgatc
      901 gctcaggcgg cccggcggtc ctcctcctcc cccggctcct cccactccat ctcgcgagac
      961 cagtacacgg cggtcccgac gatgagcccc agcagcagtg cgacgaagcc ccaggcgagt
     1021 cccgacagcc ccaggccgag cgccgcgtcc agcgagatcg accccgggcc gacgaaggcc
     1081 agcgccgcag cggcggccgc gttgaccagc gggtactcga agcccccgtc ctggttgaag
     1141 aagccgttcc gcagatggac ggtgagcgtg gcgttcagca tcacccccac ggtggcggcc
     1201 gcccccagcg gggtgaggag cccgaacgcg aggagcagac cgccccccgt ctcggccagg
     1261 ccggccagcg ccgcggcgag ccggcctcgc gggtagccca gctgctcctc gtagaagcgg
     1321 ccggtgtcgt cgagtccgtg cccgccgagc catccgaaga gcttctgggt cccgtgcgcg
     1381 acgaaggtcc cgcccagcac gagacgcagg agcaagagtc cgatatccat ggaaaacccc
     1441 cttcgtcctg tgcgcagggg tacctatcca gcgagggcgc gcctcacacc gccgtcacga
     1501 gattcaccag gcggggcggc cgggcgatca cgcgcgagac ctcgcgccca cccaaggccc
     1561 gccggacctt ctcggaggcc cgggccagct cgaccgcctc ctcctcgtcc acgtcggccg
     1621 gcacgtcgat cgtgtcccgc accttcccgt ctacctggac cacgagcgtg acggtctcct
     1681 cccgggccag gtcgggatcg aacgagggga agtcctggac gtgcacgctc tccgtccggc
     1741 cccacgtccc ccgccagatc tcctcggtga tgaacggggc catcggcgcg agcatcagca
     1801 ccagggcctc ggcggcacgc cgggcgctcc ctccctcgtc cagcacccgc cggatctcgt
     1861 tcgtgagctc catcagccgc gccacggcca cgttggtccg gaaccgctcg tagagggccg
     1921 cgacctcgcg gatcgtgcgg tgcgtcaggc gccggaggtc ctcggggtcc tcgccctcct
     1981 ggtcggccac ggccagcccc acgcgccaca cccgtcccag ccacgagtgc acgccggccg
     2041 gcgagaccgt ggcccagtcg acgtcgtcct cgacgggccc cgcgaacagc atcgtcacgc
     2101 ggagcgtgtc gaccccccac ttctcgatca ggggaagggg ctcgacgagg ttcccccgcg
     2161 acttcgacat cttggccccc tgcatgatga ccatcccctg gttcagcagc cgggggaagg
     2221 gctcgcggaa cgacaccatc cccatgtcgt gcagcacctt ggtgaggaac cgcgagtaca
     2281 ggaggtggag gatcgcgtgc tcgatccccc ccgtgtactg gccgaccggc atccaccgct
     2341 ccacggcctc gggatcgaac ggtcgatcgt cctcgtgcgg cgagcagtac cggaagaagt
     2401 accaggacga atccacgaac gtgtccatcg tgtccgtctc gcgccgcgcg gcgccgccgc
     2461 agcgcgggca cgacacgttc acgaacccct cgtgcttggc caggggcgac tcgcccgtcg
     2521 gggcgaagtc cacatcctcg gggaggagaa cgggcaggtc ctcctccggc accggcacct
     2581 cgccgcactc ggggcagtgg atgatcggga tcggcgcgcc ccaataccgc tgcctggaga
     2641 tcagccagtc gcggaggcga tacgtcaccg ccgggcggcc gcgccccgtc tcctcgagcc
     2701 aggccgtgac ccgcccgatc gacgccggcg agggggtgcc gtcgaacgct ccggaccgca
     2761 ccatcacgcc ctcgtgcgga tacgcctcgg tcatctcctc ggcctcgagc tcctcaccct
     2821 ccggctggat cacgacgcgc acctcgagac cgtgctcgcg agcgaactcg aagtcccgct
     2881 ggtcgtgccc gggcacgccc atgaccacac cggtcccgta ctccatcagg acgtagggag
     2941 ccacgaagat cggcaccttc tcgccgttca cggggttcac ggcgtgcacg cccagcccga
     3001 tgccctcgcg ggactcgccc aggtctcgcg aggcaagcgg cgtggagcgc acccactcca
     3061 gcagctcggc ggcctcggcc gacgttcccc ctcgctcggc gagcgcgggc accagctcgt
     3121 gctcgggcgc gaagaccatg aacgtcacgc cccagagcgt gtcagggcgg gtcgtgaaga
     3181 cggtcacccg gtcgcccgtc tcggcgatct cgaactcgac ctccgccccc tccgagcgcc
     3241 cgatccagtt ccgctgcatc gtcaggactc ggtcggacca gtcctcgagc agctccatgt
     3301 cgtcgaggag cgcctccgcg tactcggtga tccggaagaa ccactgcgtg aggtccttcc
     3361 gaacgacggg cgtcccgcac cgctcgcagg cgccgttcac gacctgctcg ttggccagca
     3421 ccgtctggtc cttggggcac cagttcgcgg gcgcgagctt ccggtaggcg aggccccgct
     3481 cgtggagcct caggaacagc cactgggtcc agcggtagta ctccgggtcg caggtgttga
     3541 ggcgacgggt ccagtcgaac gacacgccgt accgcttgaa cgacgcggcc tgcttgtcga
     3601 tgttctcgta ggtccacgtc ttcggatgga tcccccgctg gatcgcggcg ttctccgccg
     3661 gcagtccgaa cgcgtcccac ccgatcgggt gcaggacgtt caggccgcgc atccggtgga
     3721 agcgggccac cacgtcgccg atggcgaacg cctccgcgtg acccatgtgg atgtcgccgg
     3781 acgggtacgg gaacatgtcg agcgcgtaga agcggggacg gggatccccc tcgcccccgg
     3841 cctcgtacag ccgctcatcc tcccaggcgg caacccacct gggctcgatc tcctctggtc
     3901 gatactcgtt catggagcct tcccgccggg tacgagcaag gaaagggccc ggaccgttgt
     3961 caccccggca ccctgtcgat atcgtatcgt gagccccggc ccctgacggc cccgagcaca
     4021 tggggaagga catctccgcc gacgagtcga agtcgaagcg cgcatccgag cccgaggcca
     4081 tcccccgcgg gaacggggat cgggccgtgc ccgccccggc ccccgaaccg gtcgaggccc
     4141 cccgcgaacg gcgtcggcct ttgcccgtgc tggccaacaa ggtgggccat ttcctggccg
     4201 ccttcttcct cctggtgctg tcgatcgagc tgatgaaggg gggcgccgag gccatcgccc
     4261 cccggctcca gggcaccttc cccgtcgaca acatggtcag cacgatgggg ctgggctggc
     4321 tgggcgccta cctcgtcctc tcgggatcac cggtcgccgc cgcgtcgctc gccctcttca
     4381 cggccggggc cacgacccag gcccagacct tcaccatgct ctccggctcg cggctgggcg
     4441 cctcgttcat cgtcctcctc gtcgggttcc tctacgcgat gcgccagcgc aaccggcatc
     4501 ggcggcggga gagcatcggg atgggggtcc tggccctggg gatgacggcc gtcgtgtacg
     4561 tgcccgggat gttcctgggc tacgggatcc tcaagtcggg cctcctctcg ggcgtcacgt
     4621 ggcgctcctc ggccgggatc aacgggatcg tggacgccgt ctgggggccg atcacccgct
     4681 tcgcgtcgga gctccttccg gggccgctgc tcttccccct gggcctggcc gtcatcctcg
     4741 cgtcgttcaa actgctcgac ttcgtgctgc cggcgctcga cggggagaag cacgccgggg
     4801 agcgcggcca ctggatgaag cacccatggg cgatgttcgg gctcgggatg ctcgccgcac
     4861 tgctcaccct gtcggtgtcg gtggcgctca cgatcctcgt cccgctggcg agcaagggct
     4921 acatcgagcg gcgcgaggcg atcccctaca tcgcgggggc gaacatcacc acgctggccg
     4981 acacgctctt cgcggccatg ctgctgggca accccatcgc cgtgcagatc gtcctggcgc
     5041 aggcgatcgc ggtggccatc gtcaccgtgc tcctcctggc cttcctctac cgtccgctcc
     5101 agacgatgat catggcactg gacgagtgga tcgtgcgcac cacgccgcgc ctcgtcgcgt
     5161 tcgtggcgat cctcttcgtg atgccgatcg gcctgctctt cagcgggagg atgctctgac
     5221 ggctcacagg gagccgcgga gcttcgcgtc cccgaaggcc gcgaccctcc ccgtccgccc
     5281 cacgacccag tagccggaac ccgagggcga cccggcgatg gcggcgaccg ggcccaggcc
     5341 cttcttggcg gcctggccgt gcagctgggc cgagccgaag gcgtggacgt tgcccttgcg
     5401 ggtgacgagc cagtagccgt ccggaccgct cacggccccg cccacgaccg gcgcggcgag
     5461 agacgcatcg gccagatctc cccgatgctc cgcgtctccg aaggcgaaga cctcgccccc
     5521 ggccgcgtag acccagtagc catgccccga gggcgtgggg acgatcccgg acaccgcagc
     5581 ggcccccttc tccacccggc cgaagcggcc ggcgtcgccg aaggccgcga ccttcccctg
     5641 cttggtcagg agccagtacc cctgccccgt cggcgtggcc gcgaacccga cgatgacggc
     5701 cttcttccgc gtcacccggc cgtggagctc ggcctcgccg aacgcgatca cgcggcccga
     5761 ccggaggacg agccagtacc cgtcatcgtc cgggcgggag gccatctcca cgatgggcgc
     5821 cggcgggaac ctccccgcct tggagccgtg gaacggagcg gcgtaggccc gcacgctccc
     5881 gtcccccccc gccagccagt acccgccgtc gctcgaggcc gccatcccgg tgatgaacga
     5941 ccgctcgggg gtgaagaagc tcgtgcgctt ccgggctccc tgccacgaga agctgaagtg
     6001 gacgtggtcg tcgtgcgggt gggtgaagga ccccgacggc gtcttgcatc cccggggctt
     6061 ctggacgcag taccgcttcc aggtcccctc ccacgacgtc caccacctgc ggttccagat
     6121 cacgtacatc agcccgagcc tccgggccat cgcgttggcg ttcccccggg cgtcgtccgc
     6181 gaacatccag gcgaagaggt ccttcacctt cctgcggtcc ttgggcctgg aggcgttcat
     6241 cgcccagtcg agggcgcgcc cctccttgtg ctcgctcttc ccgcccacgc tgcacgcgcg
     6301 gctcgagttc atccacgtct cgccgtaggt ggaaacgagg agcttgcgga acgccgtgac
     6361 gccgggcttc tccttgggcg cgcagcggct ctggccctcg taggcggcgt ggccctcgat
     6421 cagcgggccg aaccggggtg tttgcggcgc tgctcgcgcc gagggggtcg caaggagacc
     6481 cgtcagcgcg agcagcgccg ctgcgagcac ggtcgagcgc cgaataggag tgatgtccgc
     6541 aggcatgggg ttccccctgt cctacggcat cactcggagg atcgcaagcc cgacttgagc
     6601 cgggcgcggc ctcagaagat gctgacgccg ccgggcccga tgaggaggcc caggacgatg
     6661 agca
//
LOCUS       DASUTW010000205         5915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_63695, whole genome shotgun sequence.
ACCESSION   DASUTW010000205 DASUTW010000000
VERSION     DASUTW010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63695"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1507
                     /locus_tag="VF058_07085"
     CDS             <1..1507
                     /locus_tag="VF058_07085"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6208107.1"
                     /translation="RTLYRASRVHTLSPYGGGSWLLVDERHVERAGSGEPPQADRVVD
                     LPGTTIIPGFVDAHVHLSATGMKGEGLDLSAAGSKDELLSIVRDYAGDRDGSVLGAGF
                     DESSWEDPTLPTISELDGVSLHPIVLVRTDGYLSLANERALETSGALQHEGAERDQTG
                     RPTGLLRKAANSQVQRWYHASLEPAAVQDAQLRAAGLAASRGVTTVQEMAMPDKQGRR
                     DVEVLLGHLQRLPVDCIVYIADMDLPWVMEHGFPRAGGDLTLDGSIGARTAAVLEPYE
                     DGEGSGALQYDDDTLVEFLHNAHLAGLQVGLHVIGDAAIEQALRGWERVYHALDSRGR
                     RHFRARRHRLEHFEMASLQHVERAADLGLAISIQPVFDAFWGHAGGMYEQRLSPERAW
                     PMNPFRELMMRGLEVGAGSDTPVTPLDPMHGIWALENHHDPAQRMGRDDAIRLFTVGA
                     ARIGHLEKKGRLEPGSSADFAAYEVDPFDVPDPRELRPVLTVSRGREVFAR"
     gene            1533..2447
                     /locus_tag="VF058_07090"
     CDS             1533..2447
                     /locus_tag="VF058_07090"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6208108.1"
                     /translation="MASRPTQRRRDAGFLLLLTPLIWGLTFPAGKEALERLPVLPFTA
                     WSRLMGVVTLALTLPFLARKGLQPRRLFAPGVVLGGLMFVAFLLQSAGLQRTSATNAG
                     FVTGLYVVFTPLLGLLLFRRPVTRAAWVAVGISVVGLALLSVPGLGDLAPRPGDLLVL
                     ASAVVWAGHVVAVGYFAGRHPALPLALAQLVGAAGLHLIATAGEGLHVADAIQVWPLL
                     VITGVLGSGVAFTIQVLAQGTVSATRAAIILAGESVVAAIAAAIWLGERLEPHQWVGA
                     ALAVAAMILSEVGARRRPEVRLDPATAV"
     gene            complement(2468..3742)
                     /locus_tag="VF058_07095"
     CDS             complement(2468..3742)
                     /locus_tag="VF058_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208109.1"
                     /translation="MTRMTLPPVEPPDLWPEIEQRLSDPRPVHRVAGRLAAAVVALLV
                     FVSSGLLLWRAFTPERNDAVGLASGALDAIPGGWTRLPDPPEVRLGASYVWTGTELLA
                     WGGCDPSVREECAATADGYALDPTVGRWSRLAAAPAEGAHSAAIWTGSEALFLALDGP
                     VRGVALDPERRAWRSIAEGPLDGRTGAIPVWTGSRVFVWGGGEASLSSSDGAMYDPGS
                     DEWTVVAPSPISLNSASGVWTGDEVIVFGSLLGQGNRARTETAVGAAYDPEADAWRTL
                     PASRLSPQATSAVWVGGRMVAWDYEPRSQEYDPGNDRWGPPIEMPFEFDECYPDSLAL
                     GDLVFAFYCGRAALYDTVGGTWEEIDGGIVEEEIWSDAYERFLKVWRFADLVPAGPVA
                     FLAAEGITLNPKGVACYGCPGSPTSLWAFRPG"
     gene            complement(3746..4216)
                     /locus_tag="VF058_07100"
     CDS             complement(3746..4216)
                     /locus_tag="VF058_07100"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX6208110.1"
                     /translation="MLDDEERRRLESAYRDFGGEVWRAIHAYAGGRRDVADEAVAEAF
                     AQAGRRIEHIRSLRPWVFRAAFRIAAAELARGRHAAPLTELGDARELEDGSGDILDLL
                     ESLSPRQRRVFLLREILGFSTMESADLLGSSVVSVRVHLHAARRRLRESLSEGI"
     gene            complement(4339..5808)
                     /locus_tag="VF058_07105"
     CDS             complement(4339..5808)
                     /locus_tag="VF058_07105"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6208111.1"
                     /translation="MTEEPWPGFKVSTLSYVVSLMPERIIKDLELRKHGFEVYPIDPA
                     YFSPFPDGRGVLIWEDPAKAAKEIAKLNEDDARAYLRYEHDLEELASIVRPLLLRVPP
                     SVELGSWADLREAIGLGGYFFRRRRNLAKLADLMTLSVADFLDQYFVDEAVKGALSPG
                     GVIGAWGGPMTPGSAYVLLHHRMGDAAGPHGAWAFVRGGMGALSGAIARAAAAAGAEI
                     RTGAPVKRIAVTGERARGVELEDGTTIAARAVVSAAHPHLTFFDLVGREHLPAGIVED
                     LERYRTRGASAKVNLALSQLPDFTAMPGTELGPQHPEFIICPSVPYLERAWDDAKYGR
                     WSAEPMLDCVIPSTKDPTLAPDGSHVLTAFVQYAPYELEEGKTWDDEREAFGDRVVET
                     IAAYAPNVPDAILHRQVITPLDLEREYGLVGGNIFHGEMSLDQLFSLRPTPMMARHRS
                     PIRGLYLAGSGTHPGGGVMGAPGFNAARVVRRDLRLRRG"
     gene            complement(5834..5902)
                     /locus_tag="VF058_07110"
                     /pseudo
     CDS             complement(5834..5902)
                     /locus_tag="VF058_07110"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
BASE COUNT          841 a         2207 c         2047 g          820 t
ORIGIN      
        1 gcgaaccctc tatcgagcgt cacgcgtcca cacactgtcg ccctacggcg ggggctcgtg
       61 gctgctcgtg gacgagcgcc acgtggagcg ggccggatcg ggcgaaccgc cccaggccga
      121 ccgcgtcgtc gatctccccg gcacgacgat catcccaggc ttcgtggacg cccacgtcca
      181 cctctcggcc acggggatga agggcgaggg gctcgacctc tccgcggcgg gctcgaagga
      241 cgagctgctg agcatcgtgc gcgactacgc cggcgaccgg gacggctccg tcctcggagc
      301 cgggttcgac gagtcctcct gggaggaccc gacgctgccc acgatctccg agctggacgg
      361 cgtctcgctg caccccatcg tgctggtgcg gaccgacggc tacctcagcc tggccaacga
      421 gcgcgcactg gagacctccg gcgccctcca gcacgaaggc gccgagcgcg atcagacggg
      481 ccgcccgacc ggcctcctcc ggaaggccgc caactcccag gtccagcgct ggtaccacgc
      541 ctcgctcgag cccgccgccg tccaggacgc gcagctgcgg gcggcggggc tggccgcctc
      601 gcggggcgtc accaccgtcc aagagatggc gatgcccgac aagcagggac gccgcgacgt
      661 cgaggtcctc ctgggccacc tccagcgcct ccccgtcgac tgcatcgtct acatcgccga
      721 catggacctc ccgtgggtga tggagcacgg cttccctcgc gccggcggcg acctcacgct
      781 cgacggttcg atcggggcca ggaccgccgc cgtgctcgag ccctacgagg acggggaggg
      841 cagcggcgcc ctccagtacg acgacgatac cctggtcgag ttcctccaca acgcccatct
      901 ggcaggcctc caggtcggcc tccacgtgat cggcgacgcc gccatcgagc aggcgctccg
      961 gggctgggaa cgcgtctacc acgcgctcga ctcgcgtggc cgccgccact tccgtgcccg
     1021 gcgccaccgt ctggagcact tcgagatggc ctcgctccag cacgtggagc gggccgcgga
     1081 cctggggctc gccatctcga tccagccggt cttcgacgcc ttctggggcc acgccggagg
     1141 gatgtacgag cagcgcctga gtccggagcg ggcctggccg atgaacccct tccgcgagct
     1201 catgatgcgc gggctcgagg tgggcgcggg atccgacacg ccggtgacgc ccctcgaccc
     1261 gatgcacggg atctgggccc tggagaacca ccacgacccc gcccagcgca tggggcgcga
     1321 cgacgcgatc cgactcttca ccgtcggcgc cgcacgcatc ggccatctgg agaagaaggg
     1381 aaggctcgag ccggggtcct cggccgactt cgccgcctac gaagtggacc ccttcgacgt
     1441 ccccgacccg cgcgagctcc gtccggtcct gaccgtctcg cgcggccgcg aggtgttcgc
     1501 gcgatgaggc cggggcgcta gcccgaccca ggatggcgtc gagaccgacc caacgccggc
     1561 gggacgccgg gtttctcctt ctgctcacgc ccctgatctg ggggttgacc ttccccgcgg
     1621 ggaaggaggc gctcgagcgg ctgccggtcc ttcccttcac ggcgtggagc cggctgatgg
     1681 gggtcgtcac gctggcgctc accttgccgt tcctcgcgcg taagggcctc cagccgcgtc
     1741 ggctgttcgc tccaggcgtg gtcctcggcg ggctgatgtt cgtggcgttc ctgctccagt
     1801 cggcggggct gcagcgcacc agcgcgacga acgcagggtt cgttaccggg ctgtacgtcg
     1861 tgttcacgcc gctgctgggg ctgctgctgt tccggcggcc ggtgacccgg gccgcctggg
     1921 tcgccgtggg gatctcggtc gtgggactcg cgctgctctc ggtgccgggc ctcggcgacc
     1981 tggcaccgcg ccccggcgac ctgctcgttc tggcgtcggc ggtcgtgtgg gcggggcacg
     2041 tggtggccgt cgggtacttc gcggggcgcc atccggcgct cccgctggcg ctggcccagc
     2101 tcgtcggagc ggcgggcctg cacctgatcg cgacggcggg agaggggctg cacgtggccg
     2161 acgcgatcca agtctggccc ctgctcgtca tcaccggggt gctggggtcg ggcgtcgcct
     2221 tcacgatcca ggtgctcgcc caggggacgg tcagcgccac ccgcgccgcg atcatcttgg
     2281 ccggcgagtc ggtggtggcg gcgatcgccg cggcgatctg gctgggggag cgacttgagc
     2341 cccaccagtg ggtcggcgcc gcgctcgcgg tcgcggccat gatcctgtcc gaggtcggag
     2401 cgcggcgccg acctgaggtc aggctcgacc ccgcgacggc cgtctgaggg gcccggtcgg
     2461 ctggccgtca gcccggtcgg aaggcccaga gcgaggtcgg cgatccgggg cagccgtagc
     2521 atgcgacccc cttcgggttc agtgtgatgc cctcggcggc gaggaacgcg accgggcccg
     2581 ccgggacgag atcggcgaac cgccacacct tcaggaatcg ctcgtacgcg tccgaccaga
     2641 tctcctcctc gacgatcccg ccgtcgatct cctcccacgt cccgcccacc gtgtcgtaca
     2701 gggccgcccg tccgcagtag aaggcgaaca ccaggtcccc gagggcgaga ctgtccgggt
     2761 agcactcgtc gaactcgaac ggcatctcga tcgggggccc ccaccggtcg ttccccgggt
     2821 cgtactcctg ggaccggggc tcgtagtccc acgccaccat ccgtcctccc acccacacgg
     2881 ccgaggtcgc ttgcggagag agccgcgacg cggggagcgt cctccacgcg tcggcctccg
     2941 gatcgtaggc ggcgccgacg gcggtctcgg tccgggcccg attcccctgc ccgagcaacg
     3001 acccgaagac gatcacctcg tctccggtcc agacaccgct cgcgctgttc agggagatgg
     3061 ggctgggggc gacgaccgtc cactcgtcgg atcccgggtc gtacatcgcc ccatcgctcg
     3121 atgagaggct cgcctctccg cctccccaga cgaagacccg ggaccccgtc cacaccggga
     3181 tcgcccccgt ccgcccatcg aggggaccct cggcgatcga acgccatgcg cgtctctcag
     3241 ggtcgagagc gaccccccgg accggcccgt ccagcgcgag gaagagggcc tcgcttccgg
     3301 tccagatggc cgccgagtgc gctccctcgg ccggcgcggc cgccaaccgg gaccagcggc
     3361 ccaccgtcgg gtccagcgcg tagccgtcgg ccgtcgcggc acactcttca cgcaccgacg
     3421 ggtcgcagcc accccacgcg agcagctcgg ttcccgtcca gacgtaggac gccccgagcc
     3481 tcacctccgg tgggtcgggg agccgcgtcc agccgccagg gatcgcgtcc agggccccgg
     3541 atgccaggcc gacggcatcg ttgcgctcgg gggtgaacgc tcgccacagg aggagtccgg
     3601 agctgacgaa gaccagcagg gccacgacgg cagcggcgag tcgtcccgcg acccgatgga
     3661 cggggcgtgg atccgagagc cgctgttcga tctccggcca gaggtcgggc ggctcgacgg
     3721 ggggcagggt catgcgagtc atgactcaga tcccctccga gagcgactcg cggagccggc
     3781 gccgcgccgc gtgcagatgc acccgaaccg agaccacgct cgaaccaagc agatcggccg
     3841 actccatggt cgagaagccg aggatctccc gtagaaggaa cacccgacgc tgacgagggc
     3901 tgagcgactc gagcaggtcg aggatgtctc cggagccgtc ctcgagctcg cgggcgtcac
     3961 cgagctcggt gagtggcgcc gcgtgacgcc cgcgcgccaa ctctgccgcc gcgatccgga
     4021 aggcggcccg gaagacccac ggccgaagcg accggatgtg ctcgatgcga cgaccggctt
     4081 gggcgaaggc ctcggcgacg gcctcatccg cgacgtcccg acgtccgccg gcgtaggcat
     4141 ggatcgctcg ccagacctct ccaccgaagt cccgatacgc ggattccagc cgccgccgtt
     4201 cctcgtcatc gagcacgccc cgccgtcctt ccggttgcac cctctacgga ggcctagcaa
     4261 ggggcttcgc taagggccgc ccaggatgcg gacgcaccat cgactgcgtc ggttcgcgcg
     4321 gtgggccgag agtcgagctc agccgcggcg gaggcggagg tcgcgacgga ccaccctcgc
     4381 ggcgttgaag cccggggccc ccatcacgcc gccgcccgga tgcgtccccg agcccgccag
     4441 gtacagccct cggatcgggc tccggtggcg cgccatcatc ggcgtgggac gcagggagaa
     4501 gagctgatcg agcgacatct cgccgtggaa gatgttgccg cccacgaggc cgtactcgcg
     4561 ctccaggtcg aggggcgtga tgacctggcg gtggaggatc gcgtcgggga cgttgggcgc
     4621 gtaggcggcg atcgtctcca cgacccggtc gccgaacgcc tctcgctcgt cgtcccaggt
     4681 cttcccctcc tcgagctcgt acggcgcgta ctggacgaac gccgtcagca cgtggctccc
     4741 gtcgggtgcc aacgtggggt ccttcgtcga agggatcacg cagtcgagca tgggctcggc
     4801 gctccatcgc ccgtacttcg cgtcgtccca cgcccgctcc agatagggga ccgagggaca
     4861 gatgatgaac tcggggtgct gggggccgag ctcggtgccg ggcatcgcgg tgaaatcggg
     4921 aagctgggag agagcgaggt tcaccttggc cgaggccccg cgcgtccggt agcgctcgag
     4981 atcctcgacg atccccgccg gcaggtgctc acgacccacg agatcgaaga aggtcaggtg
     5041 ggggtgggcg gccgacacca ccgcccgcgc cgcgatggtc gtcccgtcct cgagctccac
     5101 gccgcgggct cgctcgcccg tcaccgcgat ccgcttcacg ggcgctccgg tgcggatctc
     5161 cgcgcccgcc gcagccgcgg ccctcgcgat cgcccccgac agcgccccca tgcctccgcg
     5221 cacgaacgcc caggccccgt gcggccccgc cgcgtcgccc atccggtggt ggagcagcac
     5281 gtaggccgac cccggcgtca tcgggccgcc ccaggccccg atcaccccgc ccggcgacag
     5341 agcccccttc accgcctcgt ccacgaagta ctggtcgagg aagtcggcca ccgagagcgt
     5401 catgaggtcg gccagcttgg ccaggttcct ccggcggcgg aagaagtagc ccccgagccc
     5461 gatcgcctcg cgcaggtccg cccacgaccc cagctccacc gaagggggga cccgaagcag
     5521 cagcggccgg acgatcgagg ccagctcctc gaggtcgtgc tcgtagcgca ggtaggcccg
     5581 cgcgtcgtcc tcgttgagct tcgcgatctc cttcgcggcc ttcgcagggt cctcccagat
     5641 cagcacgccg cgcccgtcgg ggaacggcga gaagtacgcg ggatcgatcg ggtagacctc
     5701 gaacccgtgc ttccggagct cgagatcctt gatgatccgc tccggcatca ggctgacgac
     5761 gtaggagagc gtggagacct tgaagccggg ccagggctcc tcggtcaccg ccgcgccgct
     5821 cgagcacgac cacggacttg cccgccttcg ccaggtacgc ggcgcagacg agcccattgt
     5881 gcccgccccc caccaccacc acgtccgccc gtcgc
//
LOCUS       DASUTW010000206         3040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_64120, whole genome shotgun sequence.
ACCESSION   DASUTW010000206 DASUTW010000000
VERSION     DASUTW010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64120"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2964)
                     /locus_tag="VF058_07115"
     CDS             complement(<1..2964)
                     /locus_tag="VF058_07115"
                     /inference="COORDINATES: protein motif:HMM:NF033681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeM/NucH family extracellular endonuclease"
                     /protein_id="HEX6208112.1"
                     /translation="MVPARVRSRFASVGLSVLLLALLASAIPAGAASTTLVVNEIDYD
                     QPGTDAAEFLEIKNVASAGIDLDSYSVQLVNGTGGGAAVYQTFDLPDVTLPAGGYFVI
                     CANTATVPGCDLDVDPDTNLIQNGAPDAVAIRLGVAVVDTVSYEGDTGAPYTEGSGVG
                     LVDDASEGTGISRCPDGTDTDRNNVDLVSSPITPGAENDCPPPPGPGSTTLVVNEIDY
                     DQPGTDAAEFLEIKNVSDSGIGLDPYSVQLVNGTGGGAAVYQTFDLPDVMLPAGGYFV
                     VCANAATVPGCDLDVDPDTNLIQNGAPDAVAIRLGTELVDAVSYEGNTGAPYTEGSGV
                     GLVDDASEGTGISRCADGTDTDQNNVDLRLTPVSPGGTNDCPGLPPPFGVCGGPATLL
                     HEVQGSGAASPLVGESHVMEAVVVGDFQGPGQIGGFFLQEEDADADADPSTSEGVFVA
                     HTGSAVALGEVVRLRGTVGESFGLTQIASVDQLEVCSTGASVTPAEITLPVDSLDVWE
                     AAEGMSIVIPHTLHVSGNFTLGRFGEVELAVGGPLDNPTNVVPPGAPAVELQDLNNRS
                     RIQLDDGSNVQNPLPLPPYLGPDGTLRTGDTIPSLSGVLGFAFGVYEVHPTGPVEFDR
                     VQPRPESPPDVGGSMTVAAFNVLNYFTTLDGQGPICGPQQDQGCRGADSEFELERQRA
                     KLVAALAELDADVVGFMEIENHPGDVPTADLVAGINDVLGPGTYDYIATGAVGSDAIR
                     QAIIYKPGEVTPVGDFAILDQSVDPRFLDDFNRPVIAQTFRENGTDDLVTVAVNHLKS
                     KGSDCNAIGDPDLGDGQGNCNLTRLAAAEALVDWLATDPTGSGSAEALIVGDLNSYAQ
                     EDPIVAIEAAGYTDLVEEFVGAGWAEGAYSFNFQAQSGYLDHGLASPAALARVTGAAF
                     WHINADEPVALDYNDFNQPALFSPDPFASSDHDPVVIGMCETTAPVVDVTVSPDVLWP
                     PDHKYRDVTTSVSVTD"
BASE COUNT          422 a         1030 c         1115 g          473 t
ORIGIN      
        1 gtcggtcacc gagaccgacg tggtcacgtc acggtacttg tggtcgggcg gccacagcac
       61 gtccggcgag accgtcacgt ccacgaccgg agccgtcgtc tcgcacatcc cgatcacgac
      121 ggggtcgtgg tcgctcgagg cgaacgggtc ggggctgaac agcgcgggct ggttgaagtc
      181 gttgtagtcg agggccacgg gctcgtcggc gttgatgtgc cagaacgcgg ccccggtcac
      241 cctggccagc gcggcggggc tggccagccc gtgatccagg tagccggact gcgcctggaa
      301 gttgaaggag taggctccct cggcccagcc ggcgcccacg aactcctcga ccagatccgt
      361 gtagcccgcc gcctcgatcg ccacgatcgg atcctcctgg gcgtaggagt tgaggtcccc
      421 gacgatcagc gcctcggcgc tcccgcttcc ggtgggatcc gtcgcgagcc agtccacgag
      481 cgcctcagcc gcggccagcc gcgtcaggtt gcagttgccc tggccgtcac cgaggtcggg
      541 gtcgccgatc gcgttgcagt ccgaaccctt cgacttgagg tggttgacgg cgaccgtcac
      601 caggtcgtcg gtgccgttct cccggaaggt ctgcgcgatc acggggcggt tgaagtcgtc
      661 caggaaccga ggatcgacgc tttgatccag gatcgcgaag tcgcccacgg gcgtgacctc
      721 gcccggcttg tagatgatgg cctggcggat cgcgtcggag ccgaccgccc ccgtcgcgat
      781 gtagtcgtag gtgcccgggc cgagcacgtc gttgatcccc gccacgaggt ccgccgtggg
      841 cacgtcgccc gggtggttct cgatctccat gaagccgacg acgtccgcgt cgagctcggc
      901 cagcgccgcc acgagcttcg ctcgctgccg ctcgagctcg aactcgctat cggctccgcg
      961 acagccctga tcctgctgcg gaccgcagat cgggccctgg ccgtcgagcg tcgtgaagta
     1021 gttgaggacg ttgaaggccg cgacggtcat cgagccgccg acgtcggggg ggctctccgg
     1081 gcggggctgc acacggtcga actccacggg cccggtgggg tggacctcgt acacgccgaa
     1141 ggcgaagccg agaacgccgg agagcgacgg gatcgtgtcg ccggtgcgga gcgtcccgtc
     1201 gggcccgagg tagggcggga gcgggagcgg gttctggacg ttgctgccgt cgtcgagctg
     1261 gatcctgctc ctgttgttga ggtcctggag ctcgacggcg ggcgcccctg gaggcacgac
     1321 gttggtcggg ttgtcgagcg ggcctcccac cgccagctcc acctcgccga accggcccag
     1381 cgtgaagttc ccggagacgt ggagggtgtg ggggatcacg atggacatcc cctcggccgc
     1441 ctcccagacg tccagcgagt ccaccgggag cgtgatctcc gcgggggtga ccgaggcgcc
     1501 cgtggagcag acctccagct ggtccacgct cgcgatctgg gtcaggccga aggactcgcc
     1561 cacggtgcct cggagacgga ccacctcgcc cagcgcgacc gcgctccccg tgtgggccac
     1621 gaacacccct tcggaggtgg agggatcggc gtcggcgtcg gcgtcctcct cctggaggaa
     1681 gaagcctccg atctgccccg gtccctggaa gtccccgacc acgacggcct ccatgacgtg
     1741 gctctcaccg acgagcgggc tggcggcccc cgagccctgg acctcgtgga ggagcgtggc
     1801 cgggccgccg cacacaccga agggcggagg gaggcccgga cagtcgttcg tcccgccggg
     1861 gctgacgggc gtcagccgca ggtcgacgtt gttctggtcg gtgtcggtgc cgtcggcgca
     1921 gcgcgagatc ccggtcccct cggaggcgtc gtccacgagg ccgacgccgg acccttccgt
     1981 gtagggagcg cctgtgttgc cttcgtagga gaccgcgtcc acgagctcgg tcccgaggcg
     2041 gatcgccacc gcgtcggggg ctccgttctg gatcaggttg gtgtcggggt ccacgtcgag
     2101 gtcgcagccg gggaccgtgg cggcgttggc gcagaccacg aagtagccgc ccgccggcag
     2161 catcacgtcg gggaggtcga acgtctggta gaccgcggcc ccgcccccgg tcccgttcac
     2221 cagctgcacg ctgtagggat cgaggccgat ccccgagtcg gagacgttct tgatctccag
     2281 gaactcggcg gcgtccgtgc cgggctggtc gtagtcgatc tcgttgacga cgagggtggt
     2341 ggagccgggt ccgggcggcg gtgggcagtc gttctccgcg ccgggggtga tgggcgagga
     2401 caccaggtcg acgttgttcc ggtcggtgtc ggtgccgtcg ggacagcgcg agatcccggt
     2461 cccctcggag gcgtcgtcca cgaggccgac gcctgacccc tccgtgtagg gagcccccgt
     2521 gtcgccctcg tacgagacgg tgtccaccac ggccacgccc aggcggatcg ccacggcgtc
     2581 cggcgccccg ttctggatca gattcgtgtc ggggtccacg tcgaggtcgc agccgggcac
     2641 cgtggcggtg ttggcacaga tcacgaagta gccgcccgcc ggcagcgtca cgtcggggag
     2701 gtcgaacgtc tggtagacgg cggccccgcc cccggtcccg ttcaccagct gcacgctgta
     2761 ggagtcgaga tcgatccccg cgctcgcgac gttcttgatc tccaggaact cggcggcgtc
     2821 cgtgccgggc tggtcgtagt cgatctcgtt gacgacgagg gtcgtggacg ccgcgccggc
     2881 cgggatcgcg gaggccagga gggcaaggag cagcacgctc aggccgacgg atgcgaaccg
     2941 gctccgtacg cgcgcgggga ccacgacctc cggaggctac gcgacatcct tcgattgcgc
     3001 aatcggggtt cgggcccgct ccgggatccc ggagcgggcc
//
LOCUS       DASUTW010000207         5705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1358806, whole genome shotgun sequence.
ACCESSION   DASUTW010000207 DASUTW010000000
VERSION     DASUTW010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358806"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2456)
                     /locus_tag="VF058_07120"
     CDS             complement(<1..2456)
                     /locus_tag="VF058_07120"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6208113.1"
                     /translation="MFLRSLRIRGFKSFADKTVLEFGPGVSVVVGPNGSGKSNIVDAI
                     SWVLGEQGPRALRGGQMADVIFAGAQGRTPLGMAEVTLVIDNEAGLIPIDAGEIEVSR
                     TIFRSGESQYAIGGKPVRLMDIQELLSDTGIGRGLHTVIGQGHLEDALIARPEDRRRF
                     IEEAAGIAKHRRRKERAQRKLAGLDQDLLRLQDVMTELRRQLKPLKQQAEAAQKHEAL
                     TEEAEALAARMAAARLRELYRDRDRRLPLWEEGQAKRAEAEARLAELDREISGLDRRL
                     SEAEAGLEEAERAHQQALSERSAAEAALREAVRDEGEARAKVAADEGRSGRLFTLEEE
                     VRRHEAALAETLETLEQREREQEHAEREFREAEEARREAEEARRRAHEEGARRKAETE
                     ALSRSLASAEAERDRIEASLTELRASMEKLEGERAEIEAEIERLDAQETPLAQRQTEL
                     RRERERLIGELEHLSDAERRLETRRQALDARRTALTQTSGQRFLARAKYAIGMLGKLI
                     RVEPGLERAVAAALGPMADAVVYEDHGRAVAEAREAGGATLAVVEGAAGGFRILGERP
                     LLSGVEADPRVEPLVRAALKDVYLAADEKEALAKHRRHPAASFVTRAGVLVGPAVVRT
                     APRPSEDELAVKREDSNVARDLSRVRSQIAGKRGRLTQVDAELAEVQDELERADALIT
                     AAAERMGNVGGELKALRRERSMLEERRDRVEESIRSARETMTAIPPAESEVPDLPPHP
                     EAPVTLRVEVEALRRDRSRLEAGLTRARGELEELRATDPQTLREEAEARTAEREQAEA
                     ALAEVEERAREAAAARQAAAR"
     gene            2795..3319
                     /locus_tag="VF058_07125"
     CDS             2795..3319
                     /locus_tag="VF058_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208114.1"
                     /translation="MHEFEVTLVVDGPVDDDQVIDKLYEAGCSDATIGSIDGVGYVDF
                     RREAASFSEAVLSAIEDVESVPELTVLRIEPDDLVTMAEIAERLGRSRESVRLLISGR
                     RGKGDFPAPISHLRSRSRLWRWSEVAQWADIGTPEDQQRARFIASLNAALELRHQRGE
                     LAAAERAAVEALAG"
     gene            complement(3350..4528)
                     /locus_tag="VF058_07130"
     CDS             complement(3350..4528)
                     /locus_tag="VF058_07130"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6208115.1"
                     /translation="MTAARSPWPWRPFAAFGVFGLFWGAWGASLPAIKVQTGASDAQI
                     GLALLALAAAALPAMLSSGWIIDRLGDASLAPMTAAFGLAAALPGLARSPLWLGASLL
                     AVGATSGALDVTMNAVVAGDEGRSGRRLMHAAHATFSAGVVVASVSVGLARSGGAGPA
                     IILGVVAALVLGVALLVLVSGLGDVRLTERAAAPSRGALMLQTPLLILGGLCALAYLV
                     ENALQSWSALHLEETLGGDPLIGGLGPAFFAGAAGLGRLGAQGLSASLSDRQLLIVAA
                     LVGTVGTIVTALAPSTIIALGGVFLAGAGISIAAPTIFSLTGRLAPGEHRGRAMSTVT
                     ATAYLGFLLGPPMVGAVAGALDLRAAFLTVAGAAVLLGALTPLAPRSVGALRTGRASG
                     "
     gene            complement(4533..4811)
                     /locus_tag="VF058_07135"
     CDS             complement(4533..4811)
                     /locus_tag="VF058_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011570804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX6208116.1"
                     /translation="MSDLMVRAHVFVSGRVQGVFFRDSAREEASKRGVAGWARNLPDG
                     RVEAVFEGPEEAVEAMVRWCGEGTPWAKVEDVEATREDPEGLEGFRIR"
     gene            complement(4799..5644)
                     /gene="mutM"
                     /locus_tag="VF058_07140"
     CDS             complement(4799..5644)
                     /gene="mutM"
                     /locus_tag="VF058_07140"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX6208117.1"
                     /translation="MELPEVEVLRRDLEKEATGRRIESAEVRSSKNSMKLISRHHRTR
                     KDFTKRLEGRKFVRVERRGKYLILVTDDDQALVVHFGMSGRFLKGTKRQELDQHTHVY
                     MEFARGGDLRYIDPRALGRLFVVPVEELGSVRELQRVGVDPLEHSFTWPQFSEALAAR
                     KMKLKQLLMDQEFISGLGNIYSDEVLFAAGLRYDRRSDDLSSQEVRRLYRAIQETVQE
                     AIRDRGTTLEDEAYLDLFGKPGDHRDNLNVYGRKGLPCRRCRTPIETVRISNRGAYYC
                     PVCQT"
BASE COUNT          802 a         2246 c         1862 g          795 t
ORIGIN      
        1 cgagcggcgg cctggcgggc cgccgccgcc tcccgggccc gctcctcgac ctcggccagc
       61 gccgcctcgg cctgttcccg ctcggcggtc ctggcctccg cctcctcccg cagcgtctgc
      121 gggtcggtgg cgcgcagctc ctccagctcg ccccgggccc gggtcagccc tgcctccagc
      181 cgcgagcggt cccgcctgag ggcctcgacc tccacacgga gcgtcaccgg cgcctcgggg
      241 tgcgggggca ggtcggggac ctcgctctcc gccggaggga tcgccgtcat cgtctcccgc
      301 gcggaccgga tcgactcctc gacccggtcg cgccgctcct cgagcatcga gcgctcgcgc
      361 ctcagcgcct tcagctcgcc ccccacgttc cccatccgct cggccgccgc ggtaatcagc
      421 gcgtcggccc gctccagctc gtcctggacc tcggccagct cggcgtccac ctgggtgagc
      481 cgcccgcgct tcccggcgat ctggctgcga acccgggaca ggtcgcgggc cacgttcgag
      541 tcctcgcgct tgacggccag ctcgtcctcg gagggacgcg gcgccgtccg gacgaccgcc
      601 ggccccacca gtacgcccgc ccgcgtgacg aaggaggccg cggggtggcg gcggtgcttg
      661 gccagcgcct ccttctcgtc ggcggcgagg tagacgtcct tcagcgcggc tcgcacgagg
      721 ggctccaccc gggggtcggc ctcgaccccc gagagcaacg gccgctcccc caggatccgg
      781 aagccgccgg cggcgccctc gaccaccgcg agagtggccc cgcccgcctc acgcgcctcg
      841 gcaacggccc gaccgtggtc ctcgtacacg accgcgtcgg ccatcggccc cagcgccgcc
      901 gccaccgcgc gctccagccc cggctcgacc cggatcagct tccccagcat cccgatcgcg
      961 tacttggccc gggccaagaa ccgctggccg ctggtctgag tgagcgcggt ccgcctggcg
     1021 tcgagcgcct gacgcctcgt ctccagccgc cgctcggcgt cgctcaggtg ctcgagctcc
     1081 ccgatcaacc gctcgcgctc acggcgaagc tcggtttggc gctgcgcgag cggcgtctcc
     1141 tgcgcgtcca ggcgctcgat ctcggcctcg atctccgccc gctcgccctc gagcttctcc
     1201 atcgaggccc ggagctccgt gagcgaggcc tcgatccggt cccgctcggc ctcggcggag
     1261 gcgagcgacc ggctcagggc ctccgtctcc gccttccgcc tcgcgccctc ctcgtgggcc
     1321 cggcgtctcg cctcctccgc ctcgcgcctg gcctcctccg cctcgcggaa ctcccgctcc
     1381 gcgtgctcct gctcccgctc cctctgttcc agagtctcca gcgtctcggc cagcgcggcc
     1441 tcatgccgcc ggacctcctc ctccagcgtg aagagacggc cggagcggcc ctcgtccgcg
     1501 gcgaccttgg ccctcgcttc gccttcgtcc ctaaccgcct cacgaagcgc ggcctcggcc
     1561 gcggagcgtt cgctcagcgc ctgctggtgc gcccgctccg cctcctccag cccggcctcc
     1621 gcttcggaaa gccgccggtc cagccccgag atctccctgt ccagctcggc cagccgcgcc
     1681 tccgcctccg cccgcttggc ctgaccttcc tcccagagcg gcaggcgccg gtcgcggtcc
     1741 cggtacagct cgcgcaggcg cgccgcggcc atcctcgcgg cgagcgcctc cgcctcctcg
     1801 gtcagcgcct cgtgcttctg ggccgcctcc gcctgctgct tcagcggctt gagctgccgc
     1861 cgcagctcgg tcatcacgtc ctgcagccgc aggaggtcct ggtcgagccc ggccagcttc
     1921 cgctgggccc gctccttgcg gcggcggtgc ttcgcgatcc ccgcggcctc ctcgatgaag
     1981 cggcgccgat cctcgggcct cgcgatcagc gcgtcctcga ggtggccctg cccgatcacg
     2041 gtgtggagcc cacgtccgat ccccgtgtcg gacagcagct cctggatgtc catcagccgg
     2101 accggcttcc ccccgatcgc gtactggctc tctcccgaac ggaagatcgt gcggctgacc
     2161 tcgatctcgc ccgcgtcgat cgggatcagg ccggcttcgt tgtcgatcac gagcgtgacc
     2221 tcggccatgc ccagcggcgt gcgaccctgc gcgccggcga agatcacgtc ggccatctgc
     2281 ccaccccgca gcgcgcgcgg gccctgctcg cccagcaccc aggagatggc gtcgacgatg
     2341 ttggacttgc ccgacccgtt cggcccgaca accacgctga cgccgggccc gaactccagg
     2401 acggtcttgt ccgcgaacga cttgaacccg cggatccgca gcgaccggag gaacatcgcc
     2461 cggatgctac cagcgccccc ccgtcaagtg tgggaacggg aaccgcgggc ctcgtgtaga
     2521 tgttgacaaa aggcaacggt ttgttgcatg ttgtcaacgc cgtgaacagg gcccgccatc
     2581 cgaagaagga agtcgagcga gccctgaagg taggtaacgg aacaggaagg atggctggtc
     2641 gagacgagga gctccgggca ccggtggggc gtcgccgagt gcggcaaggg gtgccgcatc
     2701 tccatctggt cgacaccgaa gaatcccggg aaccacggca aggacatcag gcgcgcggtg
     2761 ggccgatgcc cacacggaga ggaggccgaa ggggatgcat gaattcgagg tcaccctggt
     2821 agtcgacggc ccggtcgacg atgaccaggt catcgacaag ctctacgagg ccggctgctc
     2881 cgatgcgacg atcgggtcga tcgacggcgt cgggtacgtg gacttccgcc gggaggcagc
     2941 ctcgttctct gaagccgtcc tctcggcgat cgaggatgtg gaatcagttc ccgagctgac
     3001 ggtgctcagg atcgagcccg atgacctcgt caccatggcc gagatcgccg agcggctagg
     3061 ccgctcacgg gagagcgtaa gactgctgat cagcggacgg cgtgggaagg gcgacttccc
     3121 cgcccccatc tcccatctcc gctcgaggtc gcggctctgg cgttggtccg aggtggccca
     3181 gtgggccgac atcggcacac ccgaggatca gcaacgcgcg cgcttcatcg cctcgctcaa
     3241 tgcagctctc gaacttcgac accagcgagg ggagctcgcg gccgctgagc gggctgccgt
     3301 ggaagccctc gccggctgac ccagccgtct gagcccagag gcccttcgct caacccgaag
     3361 cccggcctgt tcgcaacgcg cccacggacc gcggcgccag aggcgtgagc gcgccgagga
     3421 gcaccgccgc gccggcaacg gtaaggaaag ccgctcgcag gtcgagcgcg cccgccaccg
     3481 ctccgaccat gggcgggccc agcaggaagc cgaggtacgc cgtcgcggtc accgtcgaca
     3541 tcgctctccc ccggtgctcg ccgggagcga gacgaccggt gaggctgaag atcgtgggcg
     3601 cggcgatcga gatgccggca ccggcgagga acacgccgcc gagcgcgatg atcgttgaag
     3661 gggccagcgc ggtgacgatc gtcccgacgg tccccacgag ggctgcgacg atcagcagct
     3721 ggcggtccga gaggctcgcc gagagcccct gcgccccgag cctccccagg ccggccgccc
     3781 ccgcgaagaa ggcggggccg agcccaccta tcaacgggtc tccacccagg gtctcctcga
     3841 ggtggagggc gctccaggac tgcagcgcgt tctccacgag gtacgccagg gcacagagtc
     3901 ccccgaggat cagcagcggc gtctgcagca tgagggcacc tcgggacggg gcagcagcac
     3961 gctcggtcag ccgcacatcc ccgaggcccg agacgaggac gagaagggcg actcccagca
     4021 ccagcgcggc cacgacgccg aggatgatcg ccggcccggc gccccccgac cgcgccagcc
     4081 cgacggagac gctggcgacg accacccccg ccgagaacgt ggcgtgcgcg gcgtgcatga
     4141 gccgccggcc gctccgcccc tcgtcgccag cgacgacggc gttcatcgtc acgtccaggg
     4201 ccccggacgt cgcccccacc gcgagcaggc tcgcccccag ccacagcggc gaccgggcca
     4261 gcccgggcag cgcggcagcc aagccgaacg ccgccgtcat cggggccagc gacgcgtcgc
     4321 ccagccgatc gatgatccag ccgctcgaga gcatcgccgg cagcgcggcc gccgccagcg
     4381 ccagcagggc gagcccgatc tgcgcgtcgc tggcccccgt ctggaccttg atcgccggca
     4441 gcgacgcccc ccacgcaccc cagaagagac cgaagacgcc gaaggccgcg aacggccgcc
     4501 acggccaggg cgagcgcgcc gccgtcacga cctcagcgga ttcggaagcc ctcgaggccc
     4561 tcgggatcct cgcgggtggc ctcgacgtcc tcgaccttcg cccagggcgt cccctcgccg
     4621 caccaacgga ccatcgcctc caccgcctcc tcgggcccct cgaagaccgc ctccacgcga
     4681 ccgtcgggca ggttccgcgc ccagccggcc acgcctcgct tcgaagcctc ctctctcgcg
     4741 gagtcacgga agaagacgcc ctggacccgc ccgctcacga agacgtgcgc ccggaccatc
     4801 aggtctgaca gaccgggcag tagtacgccc cgcggttcga gatcctcacc gtctcgatcg
     4861 gcgtgcggca gcggcggcat ggcagcccct tccgcccgta gacgttgagg ttgtcccggt
     4921 ggtcgcccgg cttcccgaac aggtcgaggt acgcctcgtc ctccagggtg gtcccccggt
     4981 cccggatcgc ctcctggacc gtctcctgga tcgcccggta gagccgccga acctcctgcg
     5041 aggacaggtc gtccgagcgc cggtcgtacc gcagccccgc ggcgaacaac acctcgtcgg
     5101 agtagatgtt gccgagcccc gagatgaact cctggtccat cagcagctgc ttcagcttca
     5161 tcttccgcgc ggcgagcgcc tccgagaact ggggccaggt gaacgagtgc tccagcggat
     5221 cgacccccac cctctggagc tcgcggaccg agccgagctc ctcgaccggc acgacgaaca
     5281 gcctccccag ggcacgaggg tcgatgtagc gaaggtcccc gccccgggcg aactccatgt
     5341 acacgtgcgt gtgctgatcg agctcctggc gcttcgtccc cttgaggaat cggccgctca
     5401 tcccgaagtg cacgacgagc gcctgatcgt cgtcggtgac aaggatcagg tacttgccac
     5461 ggcgctcgac ccggacgaac tttcgcccct cgagacgctt cgtgaagtcc ttccgcgtgc
     5521 ggtggtgccg cgagatcagc ttcatcgagt tcttggacga gcggacctcg gccgactcga
     5581 tgcgccgccc cgtcgcctcc ttctccaggt cccggcggag gacctcgacc tcggggagct
     5641 ccactcagga cctcccctcg tgctcgaggc gctcggccgc ctccccgtcg ccttcgagga
     5701 ggcgc
//
LOCUS       DASUTW010000208         6894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_323554, whole genome shotgun sequence.
ACCESSION   DASUTW010000208 DASUTW010000000
VERSION     DASUTW010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6894
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323554"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VF058_07145"
     CDS             complement(<1..450)
                     /locus_tag="VF058_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208118.1"
                     /translation="MLHQRGRRTAAVVALVAGLTLVASAASTAPTPIDDVVLTGPQGA
                     EWDADGNDDYGSGGPCFAREGFSPAVDVENDFSNDVFDGGLLLLVNGKTFGAGIETGN
                     LSGQQLKVGPQRFNRVKVTRIERALQTSPTLRSLIRFKNPTRRDRVLR"
     gene            complement(538..2499)
                     /locus_tag="VF058_07150"
     CDS             complement(538..2499)
                     /locus_tag="VF058_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6208119.1"
                     /translation="MPRPAHDAIAAGRDALERHAWQEAYETLSAADREGSFPAEGLQL
                     LAESAWWSAHPDETIDALERAYAAYLEEGDRASAGMMAFRVAEQHGMRGAGPQAQGWA
                     SRAEKLAAEIPESPVHGWLAWIRGLMTLVMGGDEEEALAHYDRALEIADRLGDRDLTA
                     MSLHDKGHILCSRGEVTEGLALMDEVMVAAVGGELDPQATGYVYCGMIGICSRLADYR
                     RAAEWTEATTRWCERHSITGFPGICRVHRAELLRLRGALASAEEEALRACEELPQYNL
                     YSGVGHAFYEIGEIRRRMGDHTAAEKAYARAHEHGRSPQPGLSLLRLAQGNVVAAASG
                     VAQALAEAGGDRLARAPHLAAQATIALAADDLETAASAAEDLGSIVGEDSAPAMRATA
                     ACVRGAVLLARGDAAGALPELREAREGWGQVEAPYEAAEVRLLLAKAHRALGNEDAAH
                     LEARAARAAFEQLGARWAAEEAGRVLGTLASGAAPPERVRRAFMFTDIERSTDLVGVI
                     GDEAWESLLTWHDQTLRSLFASHGGDVAHHTGDGFFVSFDDADTALACAVAVQRALAD
                     HRRSHGFAPQVRIGVHLAEATRRGQDYGGAEVHKAARIAAEARGGEILASEETARVGG
                     TTVSGDPRTVSLKGISEPVRVVPVRWRDG"
     gene            2514..5363
                     /locus_tag="VF058_07155"
     CDS             2514..5363
                     /locus_tag="VF058_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6208120.1"
                     /translation="MFRLGAALRVRPGEGGLVLRFLAVMLVGLFGAALGGAAVESLFF
                     SRFGPHFLPYLYMALGVATFFVMAAMSAVLARPHPARVLAALPLALAVLLLVARAALL
                     LDQRWLYPVLWVVMMIAWTTQVMVSWALAGDVHDTRQAKRLFPLYGVGLILGGITGGL
                     VTGPLAALLGTENLLLIWAAALIAVFALGRAVAGPGVRRRRRGRRPSVLAEAGEALRQ
                     VRAAPLLRWLSLALMLFALLYFTLTLRFAAAATARFPQTEELAGFLGGFMAVSNAAAL
                     LLSLLVANRLFARFGVPAMVLALAAIYAVGFGVLVAASGFAVLVGFRFVQMVWVNGVW
                     GTGWQALYNVIPSETRARIRSFMDAGPLQAGVVLSGAMLVLADRVLPDRAVAALGVAA
                     AGLTVWAMWRARRAYGEALEVALRTGNPDVFGSEDEPFSGRAWDAEAVQAAIGGASNR
                     DPVVRRISVEVLSRLDAPPALPVLLDALSDPEAEVRAAAVRGVGRAGDPSHAAAIEAL
                     LGDPDAAVRGSAVHAMRALGGPAASASVRALLADPDPEIAATAAAALVASSDEEALDL
                     LVEMAEAAEPERRRAAVTALAETGADPGIVLERVSDPDVSVRRAAVAAAGSLEAGAAS
                     RAVLEALQDPHPSVREAAAEALRDLRGADPALLRAYVERETGEALRYDGLWRALGTDG
                     DPRIALLASAVREESLTHARNALRAAALTGDPGVVELAAANLESRDAQQRANALEMLE
                     AVGPRAIVRPIVALWEGAPEARGDRARALLRLQDDADPWLRSCAAFAAATTEGGKEME
                     TLTTLSAMDRVLFLRNVPLFADLTPRDLGRIAGVATEHLFQEGEVIAEQGEPGDEMHV
                     VVSGEIRAIVVDDAGSRQEVGRRGTGDYVGEMAIISDQPRMASLVCVGEVRTLALDRK
                     RFERILTDRPEASLAVMRVLCDRLREAHGQQPSSV"
     gene            5425..5811
                     /locus_tag="VF058_07160"
     CDS             5425..5811
                     /locus_tag="VF058_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017199277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6208121.1"
                     /translation="MSEQENRRVIERLIQCINDRKIEVMDELFHEDAEMDWPQSGEQV
                     IGGDNRRAIYGAFPQLPTITPRRMVSGGDLVVAEASLDYGGGTVYATVFIFELRDGKI
                     ERETAYWSEPFEPPDWRSRWVQVAGA"
     gene            complement(5818..6180)
                     /gene="paaI"
                     /locus_tag="VF058_07165"
     CDS             complement(5818..6180)
                     /gene="paaI"
                     /locus_tag="VF058_07165"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HEX6208122.1"
                     /translation="MIDPLSRLLGLELLDEGPGRATVQGRVSEDHLNPHGMAHGGFIF
                     SLADAALAVASNSHGPDAMAIAATIHFTRSANAGDVLTARAREVSLGGRTATYEIEVV
                     SGEGTPVALFTGTVYRRS"
     gene            complement(6191..6562)
                     /locus_tag="VF058_07170"
     CDS             complement(6191..6562)
                     /locus_tag="VF058_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007074425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HEX6208123.1"
                     /translation="MVDQEDLLVFDGACGFCTRVARWVQRRLPPGVRVVPWQRAPLDR
                     LGLTRAEAARTAWWINADGTRRAGHLAVAEALRAVGGGWGALGVAIRLPPIRWVAAGV
                     YRLVARYRGRLPGTTPAVPDR"
     gene            6623..>6894
                     /locus_tag="VF058_07175"
     CDS             6623..>6894
                     /locus_tag="VF058_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208124.1"
                     /translation="MADHREAEERERRARLLDERLSPVMAVLGILFLLVVVGQALAVP
                     GSGLRRTLATLIWVLWGAFVVEFVVRLGIAPSPGRYLRRNWWQVLLL"
BASE COUNT          839 a         2477 c         2581 g          997 t
ORIGIN      
        1 ccggaggacc ctgtccctgc gcgtggggtt cttgaaccgg atcagcgagc gcagggtggg
       61 gctcgtctgg agggcccgct cgatccgggt caccttcacc ctgttgaacc tctgtggccc
      121 caccttgagc tgctgccccg agaggttccc cgtctcgata cccgcgccga aggtcttccc
      181 gttcacgagc aggaggagcc ccccgtcgaa gacgtcgttg ctgaagtcgt tctccacgtc
      241 gaccgcgggc gagaagccct cgcgggcgaa gcagggcccg ccgctgccat agtcgtcgtt
      301 cccatccgcg tcccactcgg cgccctgggg tcccgtaagg accacgtcgt cgatgggcgt
      361 cggcgccgtc gacgcggccg aggccacgag cgtgagtccc gccacgaggg cgaccacagc
      421 ggccgttcgc cggccgcgct gatggagcat ctgcaacctc cttgatcgtt tggctgagga
      481 agcatacgcc tcgtgggggc ccgtcgatac tcccccgaac ggatgaggtc gaacggccta
      541 cccgtctcgc cagcgcacgg ggacgacccg cacgggctcc gagatccctt tcagggagac
      601 cgttcgcgga tctccggaga ccgtcgtccc gcccaccctc gccgtctcct cgctggccag
      661 gatctccccg ccgcgcgcct ccgcggcgat gcgggccgcc ttgtggacct cggccccgcc
      721 gtagtcctgg cctcggcggg tcgcctcggc caggtggacg ccgatccgca cctgaggagc
      781 gaacccgtgg gaccgacggt ggtccgcgag tgctcgctgc acggccaccg cgcaggcgag
      841 cgcggtgtcg gcgtcgtcga aggacacgaa gaacccgtcg ccggtgtggt gggcgacgtc
      901 gcccccgtgg gaggcgaaca gagatcgcaa ggtctggtcg tgccaggtca gcaggctctc
      961 ccaggcctcg tctccgatca cgcccacgag gtccgtcgag cgctcgatgt cggtgaacat
     1021 gaaggcccgt cgcacgcgct cggggggcgc cgcgccgctc gcgagcgtcc cgaggacccg
     1081 accggcctcc tcggccgccc acctcgcgcc gagctgttcg aaggccgcac gggcggctcg
     1141 agcctcaagg tgcgccgcgt cctcgttccc gagcgcccgg tgcgccttcg ccaggagcag
     1201 gcgcacctcg gccgcctcgt acggtgcctc gacctgcccc cacccctccc tcgcctcgcg
     1261 gagctcaggg agagccccgg ccgcgtcccc gcgggcgagc aggacggccc ctcggacgca
     1321 ggcggcggtc gcccgcatcg cgggggcgct gtcctcgccg acgatcgatc cgagatcctc
     1381 ggctgcggag gcggccgtct ccaggtcgtc cgccgcgaga gcgatcgtcg cctgcgcggc
     1441 gagatgcggc gcccgggcga gccggtcgcc gcccgcctcg gccagcgcct gcgccacgcc
     1501 cgaggcggct gccacgacgt tgccctgggc gaggcggagg agcgacaggc ccggctgcgg
     1561 cgagcgcccg tgctcgtggg cccgggcgta ggccttctcg gcagcggtgt gatcgcccat
     1621 ccttcggcgg atctccccga tctcgtagaa ggcgtgaccc acgcccgagt acaggttgta
     1681 ctggggcagc tcctcgcagg ccctcagggc ctcctcctcg gcgctggcca gcgcgccgcg
     1741 cagccgcagc agctcggcgc ggtggacgcg gcagatcccg gggaagcccg tgatcgagtg
     1801 ccgctcgcac caccgggtcg tggcctcggt ccactcggcc gctcgtcggt agtcggccag
     1861 ccgcgagcag atcccgatca tcccgcagta cacgtagccg gtggcctggg ggtccagctc
     1921 gccccccacg gcggcgacca tgacctcgtc catgagggcg agcccctccg tcacctcccc
     1981 gcgcgagcac aggatgtgcc ccttgtcgtg gaggctcatg gcggtgaggt cgcggtcccc
     2041 gagccggtcg gcgatctcga gcgcccggtc gtagtgggcg agcgcctcct cctcgtcacc
     2101 gcccatcacc agcgtcatga gcccccggat ccaggccagc cacccgtgga cgggggactc
     2161 cgggatctcg gcggcgagct tctccgctcg cgaggcccag ccctgggcct ggggccccgc
     2221 gccgcgcatc ccgtgctgct cggcgacgcg gaacgccatc atccccgccg acgcccggtc
     2281 cccctcctcg aggtaggcgg cgtaggctcg ctcgagcgcg tcgatcgtct cgtccggatg
     2341 agcggaccac caggcggatt cggcgaggag ctgcagcccc tccgcgggga aggaaccctc
     2401 acggtcggcc gccgacagcg tctcgtacgc ctcctgccag gcgtgacgct cgagcgcgtc
     2461 ccggccggcc gcgatcgcgt cgtgggctgg gcggggcatg tgagaatcgt acgatgttcc
     2521 ggctcggggc cgcattgagg gtccgtcccg gtgagggcgg gctggtcctc aggttcctcg
     2581 ccgtgatgct cgtcgggctg ttcggggcgg ccctgggcgg cgcggcggtg gagtcgctct
     2641 tcttctcgcg gttcggcccg cacttcctcc cgtacctcta catggcgctg ggggtggcca
     2701 cgttcttcgt gatggccgcg atgagcgcgg tcctggcccg gccccatccg gcccgggtgc
     2761 tggcggcgct cccgctcgcg ctggccgtcc tcctgctcgt ggcgcgggcg gcgctgctgc
     2821 tcgaccagcg ctggctctac ccggtcctct gggtcgtgat gatgatcgcg tggaccaccc
     2881 aggtcatggt ctcgtgggcc ctggccggcg acgtccacga cacgaggcag gccaagcggc
     2941 tgttcccgct ctacggggtg gggctgatct tgggcgggat cacgggcggc ctggtcacgg
     3001 gtccgctggc ggccctgctg ggcaccgaga acctcctgct gatctgggcg gccgcgctga
     3061 tcgcggtctt cgccctggga cgggcggtcg ccggtcccgg cgtccgccgg cggcgacgag
     3121 gccgtcgccc gtcggttctg gccgaggcgg gggaggcgct ccgacaggtg cgcgcggccc
     3181 cgctccttcg atggctgtcg ctcgcgctga tgctgttcgc gctgctgtac ttcacgctga
     3241 ccctccggtt cgcggcggcg gcgaccgctc ggttcccaca gacggaggag ctggcggggt
     3301 tcctcggcgg attcatggcc gtgtcgaacg ccgcggcgct gctcctctcg ctcctggtgg
     3361 cgaaccgcct cttcgcgcgg ttcggggtcc cggccatggt cctggcgctc gccgcgatct
     3421 acgcggtggg cttcggcgtg ctggtcgccg cctctggctt cgccgtcctc gtgggcttcc
     3481 ggttcgtcca gatggtctgg gtgaacggcg tgtgggggac cgggtggcag gcgctctaca
     3541 acgtgatccc ctccgagacg agggctcgga tcaggagctt catggacgcg gggccgctgc
     3601 aggcgggcgt ggtgctctcg ggcgcgatgc tggtcctcgc cgaccgcgtc ctccccgacc
     3661 gcgccgtggc ggccctgggc gtggccgcgg ccggcctcac cgtgtgggcg atgtggaggg
     3721 caaggcgagc gtacggcgag gcgctggagg tggcgctccg gacggggaac ccggacgtct
     3781 tcgggagcga ggacgaaccc ttctcggggc gggcctggga cgccgaggcg gtgcaggcgg
     3841 cgatcggggg cgcgtccaac cgcgaccccg tcgtccgccg gatctccgtc gaggtcctgt
     3901 cgcggttgga cgcgcccccg gccctccccg tcctcctgga cgccctctcg gacccagagg
     3961 cggaggtgcg tgcggccgcg gttcggggcg tgggacgggc gggcgatccc tcgcacgcgg
     4021 ccgcgatcga ggcgctgctg ggggacccgg acgccgcggt ccggggcagc gcggtgcacg
     4081 cgatgcgggc cctgggaggc ccggccgcga gcgcctccgt ccgagcgctc ctggcggatc
     4141 ccgatcccga gatcgcggcg acggcggcag ccgcgctcgt cgcctcgagc gacgaggagg
     4201 cgctcgacct gctggtcgag atggcggagg cggcggagcc ggagcgccgg cgcgctgcgg
     4261 tcacggccct ggctgagacc ggcgccgatc cgggcatcgt cctggagcgg gtgtcggatc
     4321 ccgacgtgtc ggtgcgccgc gccgcggtgg cggcggctgg gtcgctcgaa gccggggccg
     4381 cctcccgagc ggtcctcgag gccctccagg atccccaccc cagcgtgcgg gaggccgctg
     4441 ccgaggccct gcgcgacctc cggggggccg accccgccct gctccgtgcc tacgtcgagc
     4501 gggagacggg cgaggcgctc cggtacgacg gcctgtggag agccctgggg accgacggcg
     4561 acccgcgcat cgcgctgctg gcgtcggccg tccgggagga gtcgctgacc cacgcccgca
     4621 acgcgctgcg ggccgcggcg ctcaccggtg atccgggtgt cgtggagctg gccgcggcca
     4681 acctggagag ccgtgacgcg cagcagcgcg cgaacgcgct cgagatgctc gaggcagtgg
     4741 gtccccgagc catcgtgcga ccgatcgtgg cgctctggga gggagccccc gaggcccgcg
     4801 gcgaccgggc cagggcgctc ctgcgcctcc aggacgacgc ggacccgtgg ctgcgatcgt
     4861 gcgccgcctt cgccgcggcc accaccgaag gagggaagga gatggagacg ctgaccacgc
     4921 tgtcggcgat ggatcgggtg ctgttcctgc ggaacgtgcc gctgttcgcg gacctcacgc
     4981 cgcgcgacct gggccggatc gcgggggtgg ccaccgagca cctcttccag gagggcgagg
     5041 tgatcgccga gcagggcgag cccggggacg agatgcacgt ggtcgtctcc ggcgagatcc
     5101 gggcgatcgt ggtggacgac gcgggttccc ggcaggaggt gggccgccgg gggaccggcg
     5161 actacgtcgg cgagatggcc atcatcagcg atcagccgag gatggcatcg ctcgtgtgcg
     5221 tgggggaggt gcgcaccctg gccctcgacc gcaagcgctt cgagcggatc ctcaccgacc
     5281 gcccggaggc cagcctcgcc gtgatgcgcg tgctctgcga tcgcctgcgc gaggctcacg
     5341 gccagcagcc ctcctcggtc tgaccgggtc gactgggcct tgctctggtg tcaagatgac
     5401 ggcggtggag ggaggtgatc gggagtgagc gaacaggaga accgccgggt gatcgagcgg
     5461 ctcatccagt gcatcaacga tcggaagatc gaggtcatgg acgagctctt ccacgaagac
     5521 gccgagatgg actggccgca gtcgggcgag caggtgatcg gaggcgacaa ccgccgcgcg
     5581 atctacgggg cgttcccgca gctgcccacg atcacgcccc gtcgcatggt cagcgggggc
     5641 gaccttgtgg tggccgaggc gtcgctcgac tacggcggcg ggaccgtcta tgcgacggtc
     5701 ttcatcttcg agctccggga cggcaagatc gagcgggaga cggcctactg gagcgagccc
     5761 ttcgagcccc cggattggcg ctcgcggtgg gtccaggtgg cgggcgcctg atcgggctca
     5821 gctccgccgg tagacggtcc cggtgaagag ggcgacgggg gtcccctcgc cggagacgac
     5881 ctcgatctca taggtggccg ttcggccacc cagcgagacc tcgcgggccc gggccgtcag
     5941 gacgtcccct gcgttcgccg accgtgtgaa gtggatcgtc gccgcgatcg ccatcgcatc
     6001 ggggccgtgg ctgttcgacg cgacggcgag ggcggcgtcg gccaggctga agatgaagcc
     6061 cccgtgggcc atgccgtgcg gattgagatg gtcctcggaa acgcgcccct ggacggtcgc
     6121 gcggcccggg ccctcatcca gcagctccag gccgagcagg cgggacagcg gatcgatcac
     6181 gggctgcagg ctaccggtcc gggacggccg gcgtcgtccc gggcaggcgg ccccgatatc
     6241 tcgcgacgag ccggtagacg ccggcggcga cccaacggat cggcgggagg cggatcgcga
     6301 cgccgagggc cccccagccg ccgccgaccg cgcggagcgc ctcggctacc gcgaggtgcc
     6361 cggctcgacg ggtgccatcg gcgttgatcc accacgccgt ccgggccgcc tcggcccgcg
     6421 tgaggccgag ccggtccagc ggagcgcgct gccacggcac gacccggacc cccgggggca
     6481 gtcgccgctg gacccagcgc gccacgcggg tgcagaagcc gcacgccccg tcgaacacga
     6541 ggaggtcctc ctggtccacc acggccccac ggtaccccgc gagggttaga ccacgcccgt
     6601 tccggagact caacgatcat cgatggcgga ccatcgagag gcggaggagc gcgagcgcag
     6661 ggctcggctg ctcgacgaac ggctgagccc ggtgatggcc gtgctcggga tcctcttcct
     6721 gctcgtcgtc gtggggcagg ccctggccgt gcccggaagc gggctccggc gcaccctggc
     6781 cacgctcatc tgggtcctgt ggggcgcgtt cgtcgtggag ttcgtcgtgc ggctggggat
     6841 cgcgccgtcc cccggtcggt acctgagacg gaactggtgg caggtcctgc tgct
//
LOCUS       DASUTW010000209         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1878893, whole genome shotgun sequence.
ACCESSION   DASUTW010000209 DASUTW010000000
VERSION     DASUTW010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1878893"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VF058_07180"
     CDS             <1..889
                     /locus_tag="VF058_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6208125.1"
                     /translation="EDGLMAIDRDDRTATEVPWDDEEELEASRRTIWERVPRWITIPL
                     FGALLVLVWQLAVLMEITSEFVLPPPREVGVELLQMLGDLFTGGYVLENLWVTMQEVF
                     IGLLVAAIAGIGLGILIAETSFGRQVVLPFVVGINAAPKVAFAPIFVAWLGFGIWAKV
                     AMGAFIAFFPLLIDTAAGLASVDADQAKLFRALRASRVEMFFKLKLPNGLPFIFAGLK
                     TASVLAVVGAIVGEFLGGGAGFGSLIRIAGSQLAIARVFGFVAILSVIGYLLYAIVAW
                     VERRVVFWRSSELIPGAGM"
     gene            906..1820
                     /locus_tag="VF058_07185"
     CDS             906..1820
                     /locus_tag="VF058_07185"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6208126.1"
                     /translation="MDAHSHLYPTAWAEHGRMPPDMFDVPSLLERQEEGGVTVSVISD
                     PHIWYGGLDPGSIERTREYNDFAAELTREHGPRVVALGSVTPWRGEDHVEEARRAVEE
                     LVLPGLAMATSDDGRYLDAVPEEFWELVEGRDVPVFLHPGGTVIGQELMNEYRLAEVC
                     GRPLDTTVTLSRFILMGFMERFPTLRILCPHAGGAIATVADRLDFGHELRSYAPLGPW
                     GEVELPQPPSEYVKRLYLDTVTYGPGPLRLALERVGPGRVVYGSDNPPVPFTLSRSLG
                     VVRSLGLSEEDEAKVLGANAVTLFRLRG"
     gene            1844..>2436
                     /locus_tag="VF058_07190"
     CDS             1844..>2436
                     /locus_tag="VF058_07190"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX6208127.1"
                     /translation="MPEILENLIQAMSAGTVEVVDLTQPLSERTPIIQLPEPFANTPP
                     LVVHEISRYDDRGPAWYWNWLEMGEHHGTHFDAPIHWVSGQDKPDVSQVPPAHLVAPA
                     VVIDKSVEAAENSDYLLTIDEIRAFEEEHGALPEGGWLLYRTGWDARAHDQAEFLNAD
                     ETGPHTPGVDVECAKWLAEEAPIVGMGVETVGTDAGAA"
BASE COUNT          352 a          777 c          909 g          398 t
ORIGIN      
        1 ggaggacggc ctgatggcga tcgatcgcga cgaccggacg gccaccgagg tcccgtggga
       61 cgacgaggag gagctcgagg cctcccggcg aacgatttgg gagcgggtcc cccggtggat
      121 taccatcccg ctcttcggcg cgctgctcgt cctggtctgg caactggccg tgctgatgga
      181 gatcacctcg gagttcgtcc tcccgccccc gcgggaggtc ggcgtcgagc tgctgcagat
      241 gctgggcgat ctgttcacgg gcgggtacgt cctggagaac ctctgggtca cgatgcagga
      301 ggtgttcatc ggcctgctcg tggcggcgat cgccggcatc ggcctgggga tcctgatcgc
      361 tgagacgtcg ttcggccgcc aggtcgtcct gccgttcgtc gtcgggatca acgcggcgcc
      421 caaggtggcg ttcgccccca tcttcgtggc gtggctcggg ttcgggatct gggcaaaggt
      481 cgcgatggga gccttcatcg ccttcttccc cctcctgatc gacaccgccg cggggttggc
      541 gagcgtggat gccgaccagg cgaagctgtt ccgcgcgctc cgagcctccc gggtagagat
      601 gttcttcaag ctgaagctgc cgaacggcct gccgttcatc ttcgccgggc tgaagacggc
      661 ctccgtgctt gccgtcgtgg gggccatcgt gggcgagttc ctgggcggcg gcgcagggtt
      721 cggctcgctt atcaggatcg cggggtcgca gctggccatc gcccgggtgt tcgggttcgt
      781 ggcgatcctg tccgtgatcg gctacctgct ctacgcgatc gtggcctggg tggagcggcg
      841 cgtcgtcttc tggcgatcct ccgagctgat cccgggcgcg gggatgtgag gtgacgatgg
      901 acatcgtgga cgcgcacagc cacctgtacc cgacggcctg ggccgagcac gggcggatgc
      961 cgccggacat gttcgacgtg cccagcctgc tggagcgcca ggaggagggg ggagtgacgg
     1021 tcagcgtgat ctccgatccc cacatctggt acgggggact tgacccagga tcgatcgagc
     1081 ggacgcgcga gtacaacgac ttcgccgcgg agctgacgcg ggagcacggg ccacgggtcg
     1141 tggcgctggg gagcgtcacg ccgtggcggg gtgaggacca cgtcgaggag gcgcggcgtg
     1201 cggtggagga gctggttctg cccggactgg ccatggccac gagcgacgac gggcgctatc
     1261 tcgatgcggt ccccgaggag ttctgggagc tcgtcgaggg ccgggacgtt cccgtcttcc
     1321 tacatccggg cggcaccgtg atcgggcagg agctgatgaa cgagtaccgg ctggcggagg
     1381 tgtgcgggcg gccgctcgac acgacggtca ccctgtcgcg gttcatcctg atgggcttca
     1441 tggagcgctt cccgacgctg cgcatactct gtccgcacgc cggtggggcg atagcgacgg
     1501 tcgccgaccg actcgatttc gggcacgagc tccggagcta cgcgccgctg gggccgtggg
     1561 gcgaggtcga gctgccccag ccgccctcgg agtacgtgaa gcgcctgtac ctcgacaccg
     1621 tgacctacgg gcccgggccg ctgcgtctgg ccctggaacg ggtgggccca ggccgcgtcg
     1681 tctacggatc cgacaaccct cccgtgccgt tcacgctctc gcgcagcctc ggcgtcgtcc
     1741 ggtcgctcgg cctctccgag gaggacgagg cgaaggtcct gggggcgaac gctgtgacac
     1801 tgttcaggct caggggctga cgagctcagg agagggagtg atcatgccgg agatcctcga
     1861 gaacctgatc caggcgatgt ccgcgggcac tgtggaggtc gtggacctga cccagcccct
     1921 gtccgagcgg acgccgatca tccagctgcc cgagccgttc gcgaacacgc cgccgctcgt
     1981 cgtgcacgag atcagcaggt acgacgaccg cggccccgcc tggtactgga actggctcga
     2041 gatgggggag caccacggga cccacttcga tgcgccgatc cactgggtca gcgggcagga
     2101 caagccggac gtgtcgcagg tgccgcccgc gcacctcgtg gcgccggcgg tcgtgatcga
     2161 caagtcggtg gaggccgcgg agaactccga ctacctgctg acgatcgacg agatccgcgc
     2221 gttcgaagag gagcacgggg cgctgcccga gggaggatgg ctcctctacc gcaccggttg
     2281 ggacgcgcgc gcccacgacc aagccgagtt cctgaacgcc gacgagacgg gtccccacac
     2341 gcccggcgtg gatgtcgagt gcgcgaagtg gctggcggag gaggccccga tcgtgggcat
     2401 gggggtggag acggtgggca ccgacgccgg cgcggc
//
LOCUS       DASUTW010000210        11001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_453031, whole genome shotgun sequence.
ACCESSION   DASUTW010000210 DASUTW010000000
VERSION     DASUTW010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11001
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453031"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..532)
                     /locus_tag="VF058_07195"
     CDS             complement(74..532)
                     /locus_tag="VF058_07195"
                     /inference="COORDINATES: protein motif:HMM:NF020989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HEX6208128.1"
                     /translation="MAGARVRRTRRGTYRLKIAREERDLLRALCEQLREALDAGDAST
                     SRLFPPAHDDPELSRRYRDLVGDQLLEERLRAIDTMEGTLDASELDEEQLTAWLTTLN
                     DVRLVLGTQLDVTEDPEPLPEDHPDAQRMAVYGYLGWVVSEIVDALSEGV"
     gene            complement(532..822)
                     /gene="clpS"
                     /locus_tag="VF058_07200"
     CDS             complement(532..822)
                     /gene="clpS"
                     /locus_tag="VF058_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017620876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEX6208129.1"
                     /translation="MSTAPTELDRPRGDEDVDRDRPWLVIVWNDPINLMSYVTYVFQK
                     LFGYPREKAHRLMLQVHNEGRAVVSQGPREKAELDTSRLHAHGLWATMKKDE"
     gene            929..1921
                     /locus_tag="VF058_07205"
     CDS             929..1921
                     /locus_tag="VF058_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX6208130.1"
                     /translation="MQFGVCFAPDPSPRRWVDLAGQAEDAGFDYVWMWDSHVLWQDVY
                     PIFTLMAYETERIRMGPLVTNPVTRDPTVTASVLATLDDISGGRMDMGIGRGDSARRV
                     IGKTPVSVERMEWASSLIRDLVAGKEVDYDGTPIRLGWAHWELPVWIAAYGPKALRAV
                     GRVADGLIMQLADPYILEWSLGYVREGAEEAGRDPADIKIMSAAPTFISDDRAAAREQ
                     VRWFPALVSNHVVDLVNRYAENELPSELTDYIEARDHYDYSEHGQQGAEHADFVPDEI
                     VDRFCVIGTVDECKEKLRELQRLGVDQFNLYAMSVEGPDAIIETYGKEIIPEFT"
     gene            1934..2779
                     /locus_tag="VF058_07210"
     CDS             1934..2779
                     /locus_tag="VF058_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX6208131.1"
                     /translation="MTTVRAAIIQANANMPKDEAIEKHVELIGKAAAEGAQITGLQEI
                     FNGPYFPVEQDAKWYAEAEPEDGPTMTRMREVARQHKMVLVVPWYEEAQTGVYYNSAA
                     VIEADGTVLGKYRKTHIPHVGPCFWEKFYFKPGNLGYPVFETSVGKVGLIICYDRHFP
                     EIARALGLKGAELVFNPSATVESLSKHLWELEQPAHAVANGYWIAAINRVGVEKPLNT
                     AKFYGSSYFCDPRGQITAKASDSEDEVLVADLDLDLIREVRNTWQFMRDRRPETYGEL
                     TELLP"
     gene            2776..4083
                     /locus_tag="VF058_07215"
     CDS             2776..4083
                     /locus_tag="VF058_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX6208132.1"
                     /translation="MTDGGVEELLARHKEVMPSWLLLYYDEPIELVRGEGTRVWDGAG
                     KEYLDFFGGIVTTISGHVVPKLVEALTEQAGKIAHTSTVYLIRPMVELAERLTGLAPI
                     DPPTKAFFVGSGTEAVEAALLFATSMRRSNEVIALRNSYHGRSFGAVGITGNKGWSAT
                     SFSPLNVSYALSPYCYRCPLGLEYPDCGVACAEDLRNVIETTTTGEPAAMIAEPIQGV
                     GGFVTPPPEYFGIVKSILDEFGIPFIADEVQTGFGRTGESFWGIDSFGVRPDAIVCAK
                     GLGNGMAIGAVVGRNELVDSLRGNSISTFGGNPLASTYALANLDNIEEQDLQQNAYKV
                     GDYLMRGLKDLEDRYDVVGEVRGKGLMIGVELVKDGESKEPGAEEAGRVMQRAKDEGL
                     LVGKGGLYGNVLRLSPPLTLTEDDAARAIETLDVTFGEIERGV"
     gene            4080..5462
                     /gene="hydA"
                     /locus_tag="VF058_07220"
     CDS             4080..5462
                     /gene="hydA"
                     /locus_tag="VF058_07220"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEX6208133.1"
                     /translation="MSMRTLIKNGTVVTAAEATAADVLIEDERIASIGIGLDVQADKM
                     IDATDRYVIPGGVDVHTHYDLPFGGSFCSDDFGTGTRAAAFGGTTTSIDFAVQDYGES
                     LRQGLDRWFEKAQKAQTDYGLHMIVREVNEGVLKEMDELVGEGITSFKLFMAYPGVFM
                     LDDGSIFEAMQRAADSGALIMMHAENGGPIDVLVRQYLAEGKTEPINHGLTRPAEMEG
                     EAVHRVFAMAELAGAPAYIVHLSSRDALREVQEARDRGVAAFAETCPQYLFLSLDDMA
                     RPGFEGAKFVCSPPLRPKDHQEDLWTGLAHGDLQAVATDHAPFNYKGQKDMGKDDFAK
                     IPNGLPSVEDRVTLMFQRVVEGRMTPSRWVELIATSPAKMFGLYPRKGTIAPGFDADV
                     VVFDPTHERVLSAETHHMNVDYSCYEGMEVKGLPEVVIQRGSVLVEDGEWHGAEGDGT
                     FLPRSRFQEP"
     gene            5474..6970
                     /locus_tag="VF058_07225"
     CDS             5474..6970
                     /locus_tag="VF058_07225"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017551922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX6208134.1"
                     /translation="MSATTVGSYIGGTLREPGNGDVQVDPVFDPATGETIASLHHSTQ
                     AEIEDAVAAAAAAFPEWATTPVPDRAQVLFRFKALLEEHVDELARLVVRENGKTLPEA
                     AGEVRRAIEVVELACGAPTLLMGTNLDQIAHGIDEELIRFPVGVCAGITPFNFPNMVP
                     LWMVPVALAAGNTFVLKPSPRTPLSAMRLAELLAEAELPDGVLNVVHGGKEAVDALLT
                     HPDVAAVSFVGSVGVASYIYSTAAANGKRVQALGGAKNHLIVMDDADLERSVTGLISS
                     AFGNAGQRCLAGSVAVGVGSVGDALVEELSSQASALTLGPGLEPDTDMGPVIREERRK
                     ELIGYIDEGTEVGAALALDGRGVGPSEGFFLGPTIFDGVTPDMRLWKEELFGPVLSVM
                     RAGDIDEALKVLNASTYGNAASIFTSSGANAREFKRRAEAGMLGVNIGVAAPMAFFPF
                     TGWKNSFFGDLHATGADGFRFYTRQKVVTSRWSLPPALDHSRPEGRGH"
     gene            complement(6995..8401)
                     /locus_tag="VF058_07230"
     CDS             complement(6995..8401)
                     /locus_tag="VF058_07230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016329923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6208135.1"
                     /translation="MEWLVISFERFILLYFAVLALTYAYFGYLGVRSVIVYSRELSPL
                     ALGDHLSRDVAKPVSILIPAFNEAASIVESVRSLLSMHFPRFELVVVNDGSTDPTLEL
                     LREHFALIEVPLAYRQVLPSREVRGIYRSLRYPNLMVIDKENGGRADALNAAINAARY
                     PLVCAIDADTFLDADAVLRASRVFGEDETVVGIGGTIRPVNGAVVEDGQVTSLRVPKR
                     WIERFQVIEYARAFFVGRAAWSKLSALMLISGAFSIFRKEALVAIDGYWTETVGEDME
                     VVFRLHRHYRSLGKPYKLLFTPDPIAWTEVPSDLRSLRAQRNRWQRGMLETLWRHRSM
                     TFNPRFGRLGMLGVPHYWLFEAAAPVVEVLGYAALIVSAATGVLFAQFAVMFLLLALL
                     YSVLFSQFAAGIESFLLARYPRTRDRMILLAASFLEFIGYRQILVFERFRACFQVRRK
                     AKQWGARRRVGIREPAGR"
     gene            complement(8401..9576)
                     /locus_tag="VF058_07235"
     CDS             complement(8401..9576)
                     /locus_tag="VF058_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX6208136.1"
                     /translation="MGGFVLQAAALALLTWSFIPAASRLFPSATPTRALLGALLLTTT
                     ALLGIVGFILGYYLIGARREARRDAEVRAWAERWTGVVFGAEAPEGPLPPRALEGLLE
                     VRDKLRGSGAQALGQLFERYWSRDVLIRPLRSRRLVKRLDALDRLARARLASTFFPML
                     HATEGADARTRGMALRAAARTLALYPEGPATDEAQDAFAAALDDERISTGAALETLML
                     LEDGCSGVAMRLFDDPTPRWLRWAAIEAAGRLERVDLGLSIAALVDDLDPEIRAAALR
                     ALHGIRMIPGSAEDRVRRLLYDEVDFVRVQAARAAELLPFGSIASRMWEMLGDPSWWV
                     RRTAAEVVGGHGATGAAMLDWAARTHPDRYARHMAIQFLFDGQQIDLGTAERLRSVV"
     gene            complement(9665..10018)
                     /locus_tag="VF058_07240"
     CDS             complement(9665..10018)
                     /locus_tag="VF058_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016329925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6208137.1"
                     /translation="MPSVLVVEDDPTVARLVEMTLRFGELEVRAVGSVAEARDLLAEG
                     WDAIVLDINLPGTSGLELLRELREDLDVSTPVVMLSGQRHDDEVQRALAFEGTTYVTK
                     PFAPEELLEVVQAAV"
     gene            complement(10055..10127)
                     /locus_tag="VF058_07245"
     tRNA            complement(10055..10127)
                     /locus_tag="VF058_07245"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10091..10093),aa:Glu,seq:ctc)
     gene            complement(10157..10227)
                     /locus_tag="VF058_07250"
     tRNA            complement(10157..10227)
                     /locus_tag="VF058_07250"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10193..10195),aa:Gln,seq:ctg)
     gene            10384..10698
                     /locus_tag="VF058_07255"
     CDS             10384..10698
                     /locus_tag="VF058_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX6208138.1"
                     /translation="MNKTDLVHELVSRTDLERKDVARVVNAMLDVVRTTVAKGGRVTL
                     ADFGTFERVRRAARVGRNPHTGEAVRIPATVKPTFRPGRGFLKAAAPRRRKATRKVTR
                     RR"
     gene            complement(10682..10894)
                     /locus_tag="VF058_07260"
     CDS             complement(10682..10894)
                     /locus_tag="VF058_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208139.1"
                     /translation="MAQGTIKTYDRGSRTGSLLLDDETEVEIDPTSMEGSGIRYLRIG
                     QRVKFDLAEESGRKLARTLRIVTFAE"
BASE COUNT         1806 a         3669 c         3948 g         1578 t
ORIGIN      
        1 aacgtcgcaa cgttgctccc ggtcggcgcc cggaacgacg gagcgagggc gggcgacggc
       61 ccgccctcct ccctcaaacc ccctcgctca gagcgtcgac gatctccgac accacccacc
      121 cgagatagcc gtatacggcc atccgctgcg cgtccgggtg gtcctcgggg agcggctccg
      181 ggtcctccgt gacgtcgagc tgcgtgccga gcacgagccg cacgtcgttc agggtggtca
      241 gccaggcggt gagctgctcc tcgtccagct ccgacgcatc gagcgtgccc tccatggtgt
      301 cgatggcccg cagccgctcc tccaggagct gatcgcccac gagatcgcgg taccgacgcg
      361 agagctcggg gtcgtcgtgg gccggcggga agagccgact cgtgctcgcg tcgccggcgt
      421 cgagcgcctc ccggagctgc tcgcagagcg cgcgcaggag gtcgcgctcc tctctcgcga
      481 tcttcaagcg gtaggttccc cggcgggtgc gacggacccg cgcccccgcc atcactcgtc
      541 cttcttcatc gtggcccaca agccgtgcgc gtggaggcga gaggtgtcga gctccgcctt
      601 ctcccgaggg ccctgtgaga cgacggccct cccctcgttg tggacctgga gcatcagccg
      661 gtgggccttc tcccgcgggt acccgaagag cttctggaag acgtacgtca cgtacgacat
      721 caggttgatc gggtcgttcc acacgatgac gagccacgga cggtcgcggt cgacgtcctc
      781 gtcgccgcgc ggccggtcca gctcggtggg tgccgtggac atggccacga ggatagcggg
      841 tggctcagcg gggctcgacg aggcggtggc gcgttcgacc gtggggcgag taggctggac
      901 ccccgcgccg aggcgagagg agcggacgat gcagttcggg gtttgcttcg cgcccgaccc
      961 gtccccgcgg cgatgggtcg acctggccgg tcaggcggag gacgccggct tcgactacgt
     1021 ctggatgtgg gactcgcacg tcctgtggca ggacgtctac ccgatcttca cgctcatggc
     1081 gtacgagacc gagcggatcc gtatgggacc cctcgtgacc aaccccgtca cccgggatcc
     1141 gacggtcacc gcgagcgtgc tcgccacgct ggacgacatc tcggggggcc gcatggacat
     1201 gggcatcggc cggggcgaca gcgcgcggcg ggtgatcggg aagacgcccg tctcggtgga
     1261 gcgcatggag tgggcgtcgt cgctgatccg cgacctcgtg gcgggcaagg aggtcgacta
     1321 cgacggcacg ccgatcaggc tggggtgggc gcactgggag ctcccggtat ggatcgcggc
     1381 gtacgggccc aaggccctgc gggcggtggg ccgggtcgcg gacggcctca tcatgcagct
     1441 cgccgacccc tacatcctgg agtggtcgct cggctacgtg cgcgaggggg cggaggaggc
     1501 cggccgcgac ccggccgaca tcaagatcat gagcgccgct cccaccttca tctcggacga
     1561 ccgcgcggcc gcccgcgagc aggtgcgctg gttcccggcc ctcgtctcca accacgtggt
     1621 ggatctcgtc aaccgctacg ccgagaacga gctcccctcc gagctcaccg actacatcga
     1681 ggcccgggac cactacgact acagcgagca cggccagcag ggagccgagc acgccgactt
     1741 cgtcccggac gagatcgtcg atcgcttctg cgtgatcggc acggtggacg agtgcaagga
     1801 gaagctccgg gagctccagc gactgggcgt ggatcagttc aacctctacg cgatgtccgt
     1861 ggagggcccg gacgccatca tcgagacgta cggcaaggag atcatccccg agttcacgta
     1921 ggaggcgagg acgatgacca cggtgcgggc ggcgatcatc caggcgaacg ccaacatgcc
     1981 gaaggacgaa gcgatcgaga agcacgtcga gctgatcggc aaggccgcgg cggagggcgc
     2041 ccagatcacg ggcctccagg agatcttcaa cgggccgtac ttcccggtcg agcaggacgc
     2101 caagtggtac gcggaggccg agcccgagga cggcccgacc atgacgcgca tgcgggaggt
     2161 ggccaggcag cacaagatgg tcctggtggt gccctggtac gaggaggccc agaccggcgt
     2221 ctactacaac tccgcggcgg tgatcgaggc cgacggcacc gtcctcggga agtatcggaa
     2281 gacgcacatc ccccacgtcg gcccctgctt ctgggagaag ttctacttca agcccggcaa
     2341 cctggggtac ccggtcttcg agacctcggt ggggaaggtc ggcctgatca tctgctacga
     2401 ccgccacttc ccggagatcg cccgggcgct cgggctgaag ggcgcggagc tggtcttcaa
     2461 cccctccgcc acggtggagt cgctctccaa gcacctctgg gagctggagc agccggccca
     2521 cgcggtggcc aacgggtact ggatcgccgc catcaaccgc gtgggggtcg agaagcccct
     2581 caacacggcg aagttctacg gctcgtcgta cttctgcgac ccgcggggac agatcacggc
     2641 caaggcctcg gactccgagg acgaggtgct cgtcgcggac ctcgacctgg acctgatccg
     2701 cgaggttcgc aacacctggc agttcatgcg ggaccggaga cccgagacct acggcgagct
     2761 gaccgagctg ctgccatgac ggacggtggc gtcgaggagc tgctcgcgcg gcacaaggag
     2821 gtcatgccct cgtggctcct cctctactac gacgagccga tcgagctggt ccggggcgag
     2881 gggaccaggg tgtgggacgg ggccggcaag gagtacctgg acttcttcgg ggggatcgtc
     2941 accacgatct ccgggcacgt ggtcccgaag ctggtcgagg cgctgaccga gcaggccggc
     3001 aagatcgcgc acacgtccac cgtctacctg atccgcccga tggtggagct cgcggagcgg
     3061 ctgaccgggc tggcgccgat cgacccgccc acgaaggcct tcttcgtcgg atcggggacc
     3121 gaggccgtcg aggcggccct gctgttcgcc acgtcgatgc gccgctcgaa cgaggtgatc
     3181 gccctccgga actcctacca cgggcggtcc ttcggggccg tggggatcac cggcaacaag
     3241 gggtggtcgg cgacctcgtt ctcgccgctc aacgtgtcct acgcgctgtc gccctactgc
     3301 taccggtgtc cgctggggct ggagtatccc gactgcgggg tggcctgcgc cgaggacctc
     3361 cggaacgtga tcgagaccac gaccaccggc gagccggccg cgatgatcgc cgagccgatc
     3421 cagggcgtcg gcgggttcgt gaccccgccc cccgagtact tcgggatcgt gaagtcgatc
     3481 ctcgacgagt tcgggatccc cttcatcgcc gacgaggtgc agacgggctt cggacggacc
     3541 ggcgagtcct tctgggggat cgactccttc ggcgtccggc ccgacgcgat cgtctgcgcc
     3601 aagggcctgg ggaacgggat ggcgatcgga gcggtggtgg gccggaacga gctcgtggac
     3661 tccctgcggg gcaactcgat ctcgacgttc ggggggaacc cgctggcctc cacctacgcg
     3721 ctggccaacc tcgacaacat cgaggagcag gacctccagc agaacgccta caaggtgggg
     3781 gactacctga tgcgcgggct gaaggacctc gaggaccgct acgacgtcgt gggtgaggtc
     3841 cggggcaagg gcctcatgat cggggtcgag ctggtgaagg acggggagtc caaggagccc
     3901 ggagccgagg aggccggccg ggtgatgcag agggccaagg acgaggggct gctcgtgggg
     3961 aagggcggtc tgtacggcaa cgtcctgcgt ctctccccgc cgctcaccct caccgaggac
     4021 gatgccgcgc gggcgatcga gaccctcgac gtcaccttcg gagagatcga gcggggggtg
     4081 tgagcatgcg gaccctcatc aagaacggca ccgtggtgac ggccgcagag gcgaccgccg
     4141 ccgacgtcct gatcgaggac gagaggatcg cctcgatcgg gatcgggctc gacgtccagg
     4201 ccgacaagat gatcgacgcc accgatcgct acgtgatccc cggaggcgtc gacgtccaca
     4261 cgcactacga cctccccttc ggggggtcgt tctgctccga cgacttcggg acggggaccc
     4321 gggccgcggc gttcgggggg accaccacct cgatcgactt cgccgtccag gactacggag
     4381 agtcgctccg ccagggtctc gatcgctggt tcgagaaggc ccagaaggcc cagaccgact
     4441 acggcctcca catgatcgtc cgcgaggtca acgagggcgt cctgaaggag atggacgagc
     4501 tcgtgggcga ggggatcacg tcgttcaagc tcttcatggc ctacccgggc gtgttcatgc
     4561 tcgacgacgg gtccatcttc gaggcgatgc agcgcgcggc cgacagcggc gcgctgatca
     4621 tgatgcacgc cgagaacgga gggccgatcg acgtcctggt caggcagtac ctggccgagg
     4681 gaaagaccga gcccatcaac cacggcctca cgcgccccgc cgagatggag ggcgaggccg
     4741 tgcatcgggt gttcgccatg gccgagctgg ccggcgcgcc cgcctacatc gttcacctct
     4801 cgtcgaggga cgctttgcgg gaggtgcagg aggcacggga ccgcggcgtg gcggccttcg
     4861 ccgagacctg tccccagtac ctgttcctca gcctggacga catggcccgg cccggcttcg
     4921 agggagcgaa gttcgtctgc tccccgccgc tccggcccaa ggaccaccag gaggacctgt
     4981 ggaccgggct ggcccacggc gacctgcagg ccgtggccac cgaccacgcg cccttcaact
     5041 acaagggaca gaaggacatg gggaaggacg acttcgccaa gatccccaac gggctcccca
     5101 gcgtcgagga ccgcgtcacc ttgatgttcc agcgcgtggt cgaggggcgg atgacgccct
     5161 cgcgatgggt cgagctgatc gccaccagcc ccgcgaagat gttcggcctc tatccgcgga
     5221 aggggacgat cgctcccggg ttcgacgccg acgtggtcgt gttcgacccc acccacgagc
     5281 gggtcctctc ggccgagacg caccacatga acgttgacta ctcgtgctac gaggggatgg
     5341 aggtgaaagg cctgcccgag gtcgtcatcc agcgggggag cgttctcgtg gaggacgggg
     5401 agtggcacgg cgcggaggga gacggcacgt tcctgccgcg cagcaggttc caggagccgt
     5461 gagaggtgat gagatgagtg cgacgacggt cggaagctac atcggcggca cgctccgcga
     5521 gccggggaac ggggacgtcc aggtggaccc ggtgttcgac ccggccaccg gcgagacgat
     5581 cgcgtcgctc caccactcga cccaggccga gatcgaggac gcggtggccg ccgccgcggc
     5641 ggcgttcccg gagtgggcca ccacgcccgt gcccgaccgg gcccaggtcc tgttccggtt
     5701 caaggcgctg ctggaggagc acgtcgacga gctggcccgg ctcgtcgtca gagagaacgg
     5761 caagacgctg cccgaggcgg cgggggaggt ccggcgagcg atcgaggtag ttgagctggc
     5821 ctgcggggcc cccacgctcc tcatggggac gaacctggac cagatcgccc acgggatcga
     5881 cgaggagctg atccgcttcc cggtgggggt ctgcgcgggg atcacgccgt tcaacttccc
     5941 caacatggtg ccgctctgga tggtcccggt ggcgctggcg gcggggaaca cgttcgtgct
     6001 gaagccctcg ccccggacgc cgctctcggc gatgcggctc gccgagctgc tcgcggaagc
     6061 cgagctgccc gacggcgtgc tgaacgtggt gcacgggggg aaggaggcgg tcgacgccct
     6121 gctgacccat ccggacgtgg cggcggtctc gttcgtggga tcggtcgggg tcgcttcgta
     6181 catctactcc acggcggcgg cgaacgggaa gcgcgtccag gcgctcggcg gcgcgaagaa
     6241 ccacctgatc gtcatggacg acgccgacct ggagaggagc gtcaccggcc tcatctcgtc
     6301 ggctttcggg aacgcgggcc agcgctgcct ggcggggagc gtggccgtcg gcgtgggctc
     6361 ggtgggcgac gccctcgtcg aggagctgtc gagccaggcc tccgccctga cgctgggccc
     6421 gggcctcgag cccgataccg acatgggccc ggtgatccgc gaggagcggc gcaaggagct
     6481 gatcggctac atcgacgagg ggaccgaggt gggggcggcg ctcgccttgg acggccgcgg
     6541 cgtcggtccc tcggaggggt tcttcctcgg gccgacgatc ttcgacggcg tgacgccgga
     6601 catgcgcctg tggaaggagg agctgttcgg gcccgtcctg tcggtgatgc gcgcgggcga
     6661 catcgacgag gctctgaagg tgctcaacgc gagcacgtac gggaacgcgg cgtcgatctt
     6721 cacctcgtcg ggggccaacg cgagagagtt caagcggcgg gccgaggcgg ggatgctggg
     6781 cgtcaacatc ggcgtggccg cgccgatggc gttcttcccc ttcaccgggt ggaagaactc
     6841 gttcttcggg gacctccacg caacgggcgc ggacggcttc cgcttctaca cgaggcagaa
     6901 ggtggtcacg tcgaggtggt cgctgcctcc ggcgctcgac cactcgcggc ccgagggccg
     6961 cggccactga gcgagacctc tgcgagatgc ggggttagcg gccggcgggc tcgcggatcc
     7021 ccactcgccg cctcgccccc cactgcttcg ccttgcgccg aacctggaag cacgcccgga
     7081 agcgctcgaa gacgaggatc tggcggtagc cgatgaactc gaggaacgat gccgcgagca
     7141 ggatcatgcg atcgcgtgtg cgcgggtagc gggccaggag gaacgactcg atcccggcgg
     7201 cgaactggct gaagagcacg ctgtagagga gcgccagcag caggaacatc accgcgaact
     7261 gcgcgaacag cacgccggta gccgcgctca cgatcagcgc tgcgtacccg aggacctcga
     7321 cgacgggggc cgccgcctcg aagagccagt agtgggggac gcccagcatc ccgagcctcc
     7381 cgaaccgtgg gttgaaggtc atgctccgat ggcgccacag cgtctccagc atcccgcgct
     7441 gccaccggtt cctctgggcc cggagggatc gcaggtccga gggcacctcg gtccaggcga
     7501 tcgggtcggg cgtgaagagc agcttgtagg gcttgccgag cgaccggtag tgacggtgga
     7561 ggcggaagac cacctccatg tcctcaccaa ccgtctccgt ccagtagccg tcgatggcga
     7621 cgagcgcctc cttgcggaag atggagaacg ccccggagat cagcatcagg gccgagagct
     7681 tgctccatgc cgctcgaccc acgaagaagg cgcgcgcgta ctcgatcacc tggaagcgct
     7741 cgatccaccg cttcggcaca cgcagcgatg tgacctggcc gtcctcgacc accgccccgt
     7801 tcacgggccg gatcgtgcct ccgatcccca ccacggtctc gtcctcaccg aagacgcggg
     7861 acgcccggag gacggcgtcg gcgtcgagga acgtgtccgc gtcgatcgcg cagaccagcg
     7921 ggtagcgggc ggcgttgatc gcggcgttca gcgcgtcggc ccgcccgccg ttctccttgt
     7981 cgatcaccat gaggttcggg tatcggagcg agcggtagat gccccggacc tcgcggctcg
     8041 ggagcacctg acggtaggcg agggggacct caatcagcgc gaagtgctca cgcaagagct
     8101 cgagggtcgg gtcggttgat ccgtcgttca ccacgaccag ctcgaaccga gggaagtgca
     8161 tcgagagcag cgacctgacg ctctccacga tcgaagccgc ctcgttgaac gcggggatca
     8221 ggatcgagac gggcttggcc acgtcgcgag agaggtgatc tccgagggcg agcggggaca
     8281 gctcacgcga gtacacgatc accgagcgga cacctaggta cccgaagtag gcgtaggtca
     8341 gggccaggac cgcgaagtag aggaggatga agcgctcgaa cgagatgacg agccactcca
     8401 tcagacgacc gatcggagcc gctcggcggt gcccaggtcg atctgctggc cgtcgaagag
     8461 gaactggatc gccatgtggc gcgcgtaccg gtccgggtgg gtgcgggctg cccagtcgag
     8521 catcgcggcg cccgtcgcac cgtgtccgcc cacgacctcg gccgcggtgc gcctcaccca
     8581 ccatgagggg tctcccagca tctcccacat gcgcgaggcg atcgatccga acgggagcag
     8641 ctcggccgcg cgcgcggcct ggaccctgac gaagtcgacc tcgtcgtaga ggagcctgcg
     8701 gacccggtcc tcggcggagc ccgggatcat gcggatcccg tggagcgccc gaagggctgc
     8761 ggcacggatc tcgggatcga ggtcgtcgac gagcgccgcg atcgacaggc cgaggtccac
     8821 ccgctccagg cgacccgccg cctcgatcgc cgcccatcgg agccagcgcg gcgtggggtc
     8881 gtcgaacagg cgcatcgcca caccggagca gccgtcctcc agcagcatca gggtctccag
     8941 ggccgccccc gtcgagatcc gctcgtcgtc gagcgctgcg gcgaacgcgt cctgcgcctc
     9001 gtccgtcgcg gggccctccg gatagagggc cagggtgcgg gcggcggccc ggagggccat
     9061 gccacgcgtt cgggcgtcgg ccccttcggt ggcgtgcagc atggggaaga acgtggacgc
     9121 gaggcgggcg cgggccaggc ggtcgagcgc atcgagccgc ttgacgagcc gacgggatcg
     9181 gagcggccgg atcaggacgt ccctgctcca gtatcgttcg aagagctgac cgagggcctg
     9241 ggccccgctt ccgcgaagct tgtcacgcac ctcaaggagg ccctccagtg ccctgggcgg
     9301 gaggggcccc tcgggagcct ccgccccgaa cacgacgcct gtccagcgct cggcccaggc
     9361 gcgcacctcg gcgtcgcgcc gcgcctcgcg gcgggccccg atcaggtagt agccgaggat
     9421 gaacccgacg atgcccagca gggccgtggt cgtgaggagc agcgctccca gcagggcccg
     9481 cgtgggcgtc gcggacggga acagtcgcga ggcggcaggg atgaagctcc aggtcaggag
     9541 ggcgagagcc gccgcctgga ggacgaaacc gcccaagacg aacagggcat ataccgcatc
     9601 tccgtagcgt cgccacatac cgggttcatc ggcaggccga atcgccctct tgacctgggg
     9661 ttcttcagac ggcggcctga acgacctcga gcagctcctc cggcgcgaac ggcttggtca
     9721 cgtacgtcgt gccctcgaac gccagggcgc gctggacctc gtcgtcgtga cgctgacccg
     9781 agagcatcac gacgggcgtg ctgacgtcga gatcctcccg aagctcgcgg aggagctcca
     9841 ggccgctcgt ccccgggaga ttgatgtcga ggacgatcgc gtcccagccc tccgcgagga
     9901 gatcgcgggc ctctgcgaca gagcccacgg cgcggacctc gagctcgccg aagcggaggg
     9961 tcatctccac cagcctggcg acggtgggat cgtcctccac cactaggacg ctcggcacgc
    10021 ccggaatcgt accgcggcag ggtgtgggag agagtgaccc ctaccggatt tgaaccggtg
    10081 tctccacctt gagagggtgg tgtcctaggc cactagacga aggggccggg gcgccggggt
    10141 gagccggcgc ggtccgctgg gtcgcctgga ttcgaaccag gactagctga tccagagtca
    10201 gccgggctac cgttacccca cgacccatca cgtggtcgaa acgaccacct cgccatcgta
    10261 gcagaggctc cccgggccgc cgcgcccatc tcggccgggc ccctcgcgag ccgagcgagc
    10321 tcgttcttcc ggaagcgctc tccgcggcgt agcatcgcgg cggggcgatc gatcggagga
    10381 gccgtgaaca agacggacct ggtgcacgag ctggtgtccc ggacggacct ggagcggaag
    10441 gacgtggcga gggtcgtcaa cgcgatgctc gacgtcgtcc gtacgacggt ggccaagggc
    10501 ggtcgcgtca cgctggccga cttcgggacg ttcgagcggg tgcgccgagc ggcgcgcgtg
    10561 ggccgaaacc cgcacacggg cgaggcggtg aggatccccg ccacggtgaa gccgacgttc
    10621 cgtccgggcc gcgggttcct gaaggccgcc gcgccgagac gcaggaaggc gacgcggaag
    10681 gttactcggc gaaggtgacg atccggagcg tccgggccag ctttctcccg gactcctccg
    10741 cgagatcgaa cttcacgcgc tgcccgatcc gcaggtaccg gatccccgat ccctccatcg
    10801 acgtgggatc gatctcgacc tcggtctcgt cgtcgagcag cagggatccg gtgcggctcc
    10861 cgcggtcgta cgtcttgatc gttccttgag ccacgcgcgc tcctcaggct tgcttccgga
    10921 cggcaagtct ctccgggagg cccatctcga ggcaagtcgc cttcgtccgg ctgcgcgagg
    10981 aggaggcgag cagggcggtg a
//
LOCUS       DASUTW010000211         2568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_972553, whole genome shotgun sequence.
ACCESSION   DASUTW010000211 DASUTW010000000
VERSION     DASUTW010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972553"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..747)
                     /gene="thpR"
                     /locus_tag="VF058_07265"
     CDS             complement(154..747)
                     /gene="thpR"
                     /locus_tag="VF058_07265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX6208140.1"
                     /translation="MGRDRASRPEAKPLRLFAAADIPEDHRTAVEELIGPWRERLPGG
                     RWDPPEKWHVTLKFLGRTWPRLVERVEEACRETAASVEPFEARLTSLGVFPSPGRARV
                     LWVGLDDRTEGLRRLAAGLDERLASEFEPEKRAFTPHLTVARFRDPVRIEDPEALRRV
                     ECSAPPFEVDRLVLYRSHLLGPRGSRYEAVGEFPLGA"
     gene            complement(747..1982)
                     /locus_tag="VF058_07270"
     CDS             complement(747..1982)
                     /locus_tag="VF058_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX6208141.1"
                     /translation="MRAEIVAVGTELLLGQIANTNAKELSERLAETGLDVLHHQAVGD
                     NVDRIAEGVELALSRADVVVVTGGLGPTGDDVTREGLARAFGVELERRPEIEDFLRER
                     FRRLERDMPESNLRQALVPAGAEYVLPERGTAPGLVLRRGERVVYAIPGVPAEMREML
                     EGTILPQLRERAGETTIASRVVKTAGMAEAQVAELLDDLFEGSTNPTVAYLAGSGEVR
                     VRLTAKAASRTQALELIAPVEEEVRRRLGRAVYGSDDDLLEAVIGRMLQDRGWRVACA
                     ESLTGGALGARLTDVPGASLWFAGSAVSYTNEAKRDVLGVPQEVLDGPGAVSEECARA
                     MAEGARRLYGTEVGVALTGVAGPNEQEGVAPGTVWVAVAGGGGEEVRKVVAPGDRDGV
                     RRWAVQSALILLRKRLLEA"
     gene            complement(1982..>2568)
                     /gene="pgsA"
                     /locus_tag="VF058_07275"
     CDS             complement(1982..>2568)
                     /gene="pgsA"
                     /locus_tag="VF058_07275"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX6208142.1"
                     /translation="VRSRRLPGGRIGERIGPLGLGWANLVTVLRILLIPVVVLLILEG
                     SEGTGLAAATVFFVAAATDGLDGYLARRYDATTRTGQWLDPFADKLLISAPVVTLTAL
                     GRFPLWAAAIIVGRELAVSVLRAWLGMRGRSMPASPWGKAKTAAQILAIFLYIVPLAN
                     AGGPRLLALWLAVVLTVWSGLDYALRLQHRAPNG"
BASE COUNT          361 a         1035 c          859 g          313 t
ORIGIN      
        1 tctggtcgcg gtccacgatg ttccctcctc gctcgactcc ggcgagccta cttcgggggt
       61 ctgacatgaa actacactcc cgtgattaca cccctgtcac tccggaagcg caagacccgg
      121 acgcccgaag gctactcgaa cagatgttcg ggttcaagca ccgagcggga actcccccac
      181 cgcctcgtag cgcgaccccc ggggtccgag gaggtggctc cggtagagga cgagccggtc
      241 cacctcgaag ggcggggccg agcactcgac ccggcggagg gcctccgggt cctcgatccg
      301 cacggggtcg cggaagcggg ccacggtgag gtgcggcgtg aacgcgcgct tctcgggctc
      361 gaactccgag gccaagcgct cgtcgaggcc cgcggccagg cggcgcagcc cctcggtccg
      421 gtcgtccagg cccacccaca gcacgcgggc cctccccggc gaggggaaga cgccgaggga
      481 ggtcagccgg gcctcgaacg gctccaccga ggccgccgtc tcgcggcagg cctcctccac
      541 ccgctccacg agccggggcc aggtgcggcc caggaacttc aaggtcacgt gccacttctc
      601 gggagggtcc caccggccgc cgggaagccg ctcccgccac ggcccgatca gctcctcgac
      661 cgccgttcgg tgatcctcgg ggatgtcggc cgccgcgaag agccgaagcg gcttcgcctc
      721 cggccgcgac gcccggtccc ggcccatcag gcctccagga gccgcttccg gagcaggatc
      781 agcgccgact gcacggccca gcggcggacg ccgtcgcggt cgccgggggc gacgaccttc
      841 cgcacctcct cgccacctcc cccggcgacc gcgacccaga ccgtgccggg ggccacgccc
      901 tcctgctcgt tcgggccggc cacgcccgtg agggccaccc ccacctccgt cccgtagagc
      961 cggcgggccc cctcggccat cgcccgggcg cactcctcgc tgacggcccc cggcccgtcc
     1021 agcacctcct gcgggacccc gaggacgtcg cgcttcgcct cgttcgtgta ggagacggcg
     1081 gagccggcga accagagcga ggcccccggc acgtcggtca ggcgagcccc cagggcgccg
     1141 ccggtgagcg actcggcgca cgccacgcgc cacccccgat cctgcagcat ccgcccgatc
     1201 accgcctcga gcaggtcgtc gtcgctcccg tagacggcgc ggcccaggcg gcggcgcacc
     1261 tcctcctcca ccggggcgat gagctcgagg gcctgggtgc gggaggccgc cttcgccgtc
     1321 agccgcaccc gcacctctcc cgaccccgcg aggtaggcaa ccgtggggtt ggtcgatccc
     1381 tcgaaaaggt cgtccagcag ctcggccacc tgcgcctccg ccatccccgc cgtcttgacc
     1441 acgcgagatg cgatcgtggt ctcccccgcc cgctcccgga gctgggggag gatcgtcccc
     1501 tccagcatct cgcgcatctc ggccggcacc ccggggatcg cgtacacgac ccgctcgccc
     1561 cgtcgcagca cgagtccggg cgcggtgccg cgctcgggca ggacgtactc ggccccggcc
     1621 ggcacgagcg cctggcgcag gttcgactcg ggcatgtcgc gctccaggcg ccggaacctc
     1681 tcccggagga agtcctcgat ctcgggccgc cgctcgagct ccacgccgaa ggcccgggcc
     1741 agcccctccc gggtgacgtc gtcgccggtg ggacccaggc ccccggtcac caccacgacg
     1801 tcggcccggc tcaacgcgag ctccacgccc tccgcgatcc gatccacgtt gtccccgacc
     1861 gcctggtggt gcaggacgtc gagccccgtc tccgccagcc gctcggacag ctccttcgcg
     1921 ttcgtgttcg cgatctgccc cagcaggagc tcggtcccca cggcgacgat ctccgcccgc
     1981 atcagccgtt gggcgcccgg tgctggagcc ggagcgcgta gtcgaggccc gaccagaccg
     2041 tcaggacgac cgccagccag agcgccagca gccgcgggcc cccggcgttc gccagcggca
     2101 cgatgtacag gaagatggcg aggatctggg cggccgtctt cgccttgccc cacggggacg
     2161 cgggcatcga gcggccccgc atcccgagcc aggcccggag caccgagacg gcgagctcgc
     2221 ggccgacgat gatcgccgcc gcccacagcg ggaaccgtcc gagggccgtc agcgtcacga
     2281 ccggagccga gatcagcagc ttgtcggcga acgggtccag ccactgcccc gtccgcgtgg
     2341 tcgcgtcgta ccggcgggcc aggtaaccgt ccaggccgtc cgtcgccgcg gcgacgaaga
     2401 agacggtcgc cgcggccagc ccggtcccct ccgagccctc gaggatcagc aggacgacga
     2461 cggggatcag gaggatccgc aggaccgtga cgaggttcgc ccagccgagc ccgagcgggc
     2521 ctatcctctc cccgatccgt ccccccggaa gtcgtctcga tcgaacgt
//
LOCUS       DASUTW010000212         2126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_65245, whole genome shotgun sequence.
ACCESSION   DASUTW010000212 DASUTW010000000
VERSION     DASUTW010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65245"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            423..1502
                     /locus_tag="VF058_07280"
     CDS             423..1502
                     /locus_tag="VF058_07280"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6208143.1"
                     /translation="MDGTTVVLGFEAPALQEQVLRFIERRPGLRIAGAAGDAQALTRK
                     VREAEPQVVITTPEVLAAGPELNGAALLVVGERETTAGLRAAIRAGARGFYLWPEERD
                     SLGRDADRAGVDPERPDGRVVAVYGPRGGVGTTFLATNLAAACAGDGRRVILADLDLH
                     FADVTTALGIVANGESRTIADVAALADDLGASHLEAVLHRHPKGFEILLAPPRAEQAA
                     EVEPEQVRAMGKALRSAGDLIVLHVPRALDRVTMAALEVADEILIVVTLDVLAFRDAK
                     RAMELLAARDLSDRCRLVVNRARRAEVLPDDAERVFGLRPVAVIASDPAVPRAQDRGQ
                     VVVGRRGQAGRKVAALARSILREGS"
     gene            1499..1816
                     /locus_tag="VF058_07285"
     CDS             1499..1816
                     /locus_tag="VF058_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208144.1"
                     /translation="MTLLEQVAARVRERLQGESLVGRSPAERRLRVREEALAVLREER
                     AVLAARELGELVALVSDELTGLGPIEHLLRDPDVSEVMVNGPGAVYVERKGPNITQCV
                     ESW"
     gene            1828..>2126
                     /locus_tag="VF058_07290"
     CDS             1828..>2126
                     /locus_tag="VF058_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX6208145.1"
                     /translation="MQAFGYVRISKLDERTTSPARQRQEIERLCASRGWELSKTFEDL
                     DVSGYNGKRRPGLERMLSRMDEVDAIVFWKLDRLARGVSKFYEILQRAEASNVALV"
BASE COUNT          282 a          732 c          802 g          310 t
ORIGIN      
        1 gcgctcgccc cgacgaaggg gttcggtgtg gccacgctgc cgtccgggat cgtggtccgg
       61 accacgccct tccgtgtccg ctccgcccga actcgcttgg gagtgaacag gaggctcttg
      121 ttcgggacct tcagcgtgtc caccgcgaag ggttggtagg tcgccttgcc ccgagccccg
      181 gagagccagc ggcatcatgc ggcatccctg tgggagggca ggcttccggg gtcaggggct
      241 ggtccggtcg ggggcttccg ccccgcctgg gggagcgagg aatcacccgg cccgatcccc
      301 gagtcggccg atgcggacga gccgctcccc cgggtaccgt cttacgcacg cggaccgccc
      361 gctcccaggc ggtccgcgca ccccctcccc ctccttcccc gaagacgtga caggaggagc
      421 ggatggacgg gacgacggtg gtgctgggct tcgaggcgcc ggccctccag gagcaggtgc
      481 tccgcttcat cgagcgacgg ccgggtctcc ggatcgcggg cgcggccggg gacgcccagg
      541 ctctgacgag gaaggttcgc gaggccgagc cgcaggtcgt gatcacgacg cccgaggtgc
      601 tggccgccgg ccccgagctg aacggcgccg cgctcctggt ggtcggtgag cgagagacca
      661 ccgccgggct gcgcgccgcc atccgggcgg gggcccgagg cttctacctc tggcccgagg
      721 agcgggactc gctcggacgc gacgcggacc gagcgggggt ggaccctgag cgccccgacg
      781 gcagggtggt ggccgtgtac gggccccggg ggggcgtggg cacgaccttc ctggccacga
      841 acctggcggc ggcctgcgcc ggcgacggcc ggcgcgtgat cctggccgac ctggaccttc
      901 acttcgccga cgtcaccacg gcgctcggca tcgtggccaa cggcgagtcc cggacgatcg
      961 cggacgtggc ggccctcgcc gacgatctcg gcgcctcgca tctcgaggcc gtactccacc
     1021 ggcacccgaa aggctttgag atccttctcg ctccaccccg ggccgagcag gcggccgagg
     1081 tcgagcccga gcaggtgcgg gccatgggga aggccctccg cagcgccggg gacctaatcg
     1141 tcctccacgt gccgcgggcc ctcgatcggg tgacgatggc cgcgctcgag gtggccgacg
     1201 agatccttat cgtggtgacc ctggacgtgc tggcgttccg cgacgccaag cgtgcgatgg
     1261 agctccttgc ggcgagggat ctctccgacc gctgccgcct ggtcgtgaac cgagcccgga
     1321 gggccgaggt ccttccggac gacgccgagc gggtcttcgg cctccgaccg gtcgcggtga
     1381 tcgcctcaga cccggccgtc ccccgcgcac aggatcgggg acaggtcgtg gtcgggcgcc
     1441 gcgggcaggc cgggcgcaag gtggcggcgc tggcccggtc gatcctcagg gagggatcgt
     1501 gacgctcctg gaacaggtgg cggcgcgggt ccgggagcgc ctgcagggtg agtcgctcgt
     1561 gggccggtcg cccgcggagc ggaggcttcg cgtccgagag gaagcgctcg ccgtcctgcg
     1621 ggaggagcgg gcggtcctgg cggctcggga gctaggcgag ctcgtcgcgc tcgtctccga
     1681 cgagctgacc ggcctcggtc cgatcgagca cctcctgcga gacccggacg tgtccgaggt
     1741 catggtgaac ggcccggggg ccgtgtacgt ggagcggaag gggccgaata ttacacagtg
     1801 cgtagagtcc tggtagggtt cctgttgatg caggctttcg gctacgtccg gatctccaag
     1861 ctcgacgagc gcaccacgtc tccggcccgg cagcgtcagg agatcgagcg cttgtgcgcc
     1921 tcccgcggct gggagctctc caagaccttc gaggacctcg acgtctccgg gtacaacggg
     1981 aagcgccggc cgggcctcga gcggatgctc tcccggatgg acgaggtcga tgcgatcgtc
     2041 ttctggaagc tcgaccgcct cgcccgggga gtgtcgaagt tctacgagat cctccagcgg
     2101 gccgaagcct cgaacgtggc gctcgt
//
LOCUS       DASUTW010000213         6944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1231256, whole genome shotgun sequence.
ACCESSION   DASUTW010000213 DASUTW010000000
VERSION     DASUTW010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6944
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231256"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VF058_07295"
     CDS             <1..739
                     /locus_tag="VF058_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208146.1"
                     /translation="VLNAHPSWGAAIRGDLQVHTTWSDGRADLSEMVAAARTLGHSYV
                     GVTDHSKGLPIARGMDEETLLRELEEIDELNAGLDGFRALRSLEMNVSPQGEGDMDPE
                     VLGRLDLVLGAFHSKLRLREDQTDRYLAAIRNPTVHVLAHPRGRRYGVRAGLVADWER
                     VAEEAAATGTALEVDCFPDRQDLDVETLTLAAEADVWISIGTDAHRPEELSSFELGLA
                     SLALAGVRPERVLNLLPAEEVVAWARG"
     gene            complement(743..819)
                     /locus_tag="VF058_07300"
     tRNA            complement(743..819)
                     /locus_tag="VF058_07300"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(783..785),aa:Thr,seq:tgt)
     gene            complement(829..1566)
                     /gene="rlmB"
                     /locus_tag="VF058_07305"
     CDS             complement(829..1566)
                     /gene="rlmB"
                     /locus_tag="VF058_07305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX6208147.1"
                     /translation="MSRVLVLEGRRLALEAIRSGRALEVLLAAGARSTQGLRDVLSAA
                     EAAAVPVREVPRDRIRSLATTDSHQGVVARAEAPAALGEADLERGEWAEDARIVVLDG
                     VTDPHNVGAIARTAEAAGASALVLRRRRSAGLTPAALKSSAGALLHLPVAEVANISRA
                     LGRLKEAGFWVLGLTGEAETTIDGAAPPPGRLALVLGAEGEGLSRLVRESCDELVSIP
                     LRGRVASLNVSAAAAVALFGLPTPGRR"
     gene            complement(1563..2039)
                     /gene="ispF"
                     /locus_tag="VF058_07310"
     CDS             complement(1563..2039)
                     /gene="ispF"
                     /locus_tag="VF058_07310"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX6208148.1"
                     /translation="MTDLRVGLGFDAHPRDETRPLMLAGVRFEGEPGVAGHSDGDVVC
                     HALADALLGAGALGDIGDHFPDDEPSTEGIAGIMLLSEAVRLVRAAGLSPQRCDLVVV
                     AERPRIADRRDEMRANLAEVLGVDRDAVSVKATRPEGLGLVGDGLGCMAVATLSGP"
     gene            complement(2036..2719)
                     /gene="ispD"
                     /locus_tag="VF058_07315"
     CDS             complement(2036..2719)
                     /gene="ispD"
                     /locus_tag="VF058_07315"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX6208149.1"
                     /translation="MRAAAILLAAGRGERLGGERPKALLEVGGVSLLERAVRTVDAVP
                     AIEGFVVTVPRGLEETAKSEAASSPRFLAAVQGGDSRQASVRLGLEALPDGFDVVVCH
                     DVARPLAAPELFTAVLGALEGADGAVPVVPVTDTVKRVAGGWVAETIPRHDLALAQTP
                     QAFRRGVLEEAHRAAEAEGLSVTDDAALLERAGRRVAVVSGDVGNVKVTTPEDLERAE
                     AIVRIREGT"
     gene            complement(2719..3777)
                     /locus_tag="VF058_07320"
     CDS             complement(2719..3777)
                     /locus_tag="VF058_07320"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX6208150.1"
                     /translation="MSDRSRRRVLTGGPRGTLVELIRIILVSVFAVGGWQIAERGFEG
                     RTALLIGIALGSMVGYVVGGVIGRLTVRAVHEAEAGFEEVPAGEILAGTIGLILGLVI
                     AVLASFLIFRLPPAAAGPTIAFVAVSLSYAGYRIGRAKWADFFSLFGLRPPGAGVRPG
                     EINVVDTSALIDGRVLDVVTAGFLGGVFLVHRGVLDELQRIADSSDPQRRQRGRRGLD
                     VLHDLQRTAEVVLVDEPVRGDVDGVLVRLAKDRGGVVITSDANLAKVATALNVPVRSL
                     NELAAAVRPPVMAGEELRVHISREGREEGQGVGYLEDGTMVVVEQGRSHLGAVVVVTV
                     TNVIQTASGRLLFARLDR"
     gene            complement(3828..4346)
                     /locus_tag="VF058_07325"
     CDS             complement(3828..4346)
                     /locus_tag="VF058_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004806940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX6208151.1"
                     /translation="MLFNKGDKVIYPHHGAAVIEDLTTRELLGEKKKYFVLRLTHGDL
                     TLMVPVDNTDEVGLREVVPKREVKKVLEVLKKSETRMPANWSRRYKTNIEKIRSGDIY
                     QVAEVVRNLSIREAEKGLSAGEKRMLAKARQILISELTFAVDTPEEKAEAMVDKVLEE
                     SHKAVASANGSG"
     gene            complement(4464..5801)
                     /locus_tag="VF058_07330"
     CDS             complement(4464..5801)
                     /locus_tag="VF058_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6208152.1"
                     /translation="MGKKRTTFECTACGVPSGQWAGRCPSCGGWGTIETALPASSGSG
                     SALPPPVSLLARGNDPERRLRIGMEGVDRVLGGGLVPGSVVLVAGEPGIGKSTLLLQI
                     ADRLTGAGHGVLLASGEESHGQVAARAGRLALRSDLSFVPGRELPHVLEAASVELPAV
                     LMVDSIQAIRDPDHTSLPGGPTQVRLCADRLVGLAKAEGIIVILAGQVTKDGEVAGPR
                     TLEHAVDVVCSFDGDQRTGLRVLAGGKNRFGPEGELAWFEMGAEGLVEADPSELLTTR
                     EAEPGAAVGLLSAGRRAVAVEVQALVAPTEGSPRRHVTGLDPRRFGLVAAVVDRATGL
                     RLGRTELYGATAGGLRIEDPGCDLAVAAALASAATGVPPPGGRGFIGEVSLTGAVRGA
                     PNPGPRLAAAAAAGLTEVYAPRGVRSSDVRVRTVRHLYDALTWAAEGGGRGAA"
     gene            complement(5838..6521)
                     /locus_tag="VF058_07335"
     CDS             complement(5838..6521)
                     /locus_tag="VF058_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEX6208153.1"
                     /translation="MVRRLSRRRFLAGGLGSLLSGLRPLSGLAAAPKRVDVICRRAWR
                     AAPPREGLRRHRIRRLTVHHSAVVLSDNRDAPGRFRGHQRAHFGNGWPDIAYHVLIDR
                     GGNVYRGRSPRFRGDTATDYDPKGHLLVLCEGDFDRQPLSGRQVTALVDVLAWACRRF
                     DVKPSTIRGHGDWASTACPGRRLRRKIRSGRIRARVRERLRRGGVELQVLCGDAGRRR
                     VRRIERGRA"
     gene            6578..6865
                     /locus_tag="VF058_07340"
     CDS             6578..6865
                     /locus_tag="VF058_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208154.1"
                     /translation="MRSGPARLIISLGILALSAASCASPEEPAATRSPEAAPAATSPA
                     APTSPPPTEEPAEDPPVPGELRFTAPALGGGTIDGAEYAGSDLVIWFWAPW"
BASE COUNT          978 a         2681 c         2413 g          872 t
ORIGIN      
        1 cgtgctaaac gcccacccga gctggggggc ggcgatccgc ggcgaccttc aggtccacac
       61 gacgtggagc gacgggcggg ccgacctgag cgagatggtg gcggcggccc gaaccctggg
      121 ccactcgtac gtcggggtga cggatcactc caagggcctg ccgatcgcgc gcggcatgga
      181 cgaggagacc ctcctgcggg agctggagga gatcgacgag ctcaacgcag gactggacgg
      241 gttccgggcg ctgcgctccc tcgagatgaa cgtctcgccg caaggggagg gcgacatgga
      301 ccccgaggtc ctcgggcggc tggacctcgt cctgggagcc ttccactcga agctgcgcct
      361 gcgcgaggat cagaccgatc gctacctcgc cgcgatccgc aaccccaccg tccacgtgct
      421 cgctcacccc agggggaggc ggtacggggt ccgcgcgggg ctcgtcgccg actgggagcg
      481 cgtggcggag gaggcggccg ctacgggaac ggccctcgag gtggactgct tccccgatcg
      541 ccaggatctc gacgtggaga ccctgacgct ggcggcggag gccgacgtat ggatctcgat
      601 cgggaccgac gcgcaccgtc ccgaggagct gagctcgttc gagctcggcc tggcgtccct
      661 ggcgctggcg ggggtgcggc ccgagcgcgt cctgaacctg ctcccggccg aggaggtcgt
      721 ggcctgggcc cggggatgag cgtggagctg gcggcgggat tcgaacccgc gacctagcga
      781 ttacaaatcg cttgcgctac cggactgcgc cacgccagcg agacgattct atcggcgccc
      841 gggggtgggg agcccgaaca gggccacggc cgcggcggcg gagacgttga gcgatgccac
      901 ccggccccgg agcgggatcg agacgagctc gtcgcacgac tcccggacga ggcgcgacag
      961 gccctcgccc tccgctccga ggacgagcgc cagacgcccc gggggcggag cggcgccgtc
     1021 gatcgtcgtc tcggcctcgc ccgtcaggcc gaggacccag aagcccgcct ccttcagcct
     1081 tcccagcgcc ctcgagatgt tggccacctc ggcgaccggg aggtggagca gcgcccctgc
     1141 ggacgatttg agggccgcag gggtgaggcc ggcgcttcgg cggcgccgga gcacgagggc
     1201 ggaggcgccg gctgcctcgg cggtcctggc gatggcaccg acgttgtggg ggtcggtgac
     1261 cccgtcgagg acgacgatcc tcgcgtcctc ggcccactcc ccccgctcca ggtccgcctc
     1321 gccgagcgcc gccggcgcct ccgccctcgc caccacgccc tggtgcgagt cggtcgtcgc
     1381 gagcgaccgg atgcggtcgc gagggacctc cctgaccggg accgccgcgg cctccgcggc
     1441 cgacagcacg tcccggaggc cctgggtgct gcgggcgccc gccgccagca ggacctccaa
     1501 ggcccgcccc gagcggatcg cctcgagggc gaggcgccgc ccctcgagca ccaggacccg
     1561 gctcacgggc ccgagagggt ggccacggcc atgcacccca gcccgtcgcc gacgagcccc
     1621 agcccctcgg gcctcgtggc cttcacggac accgcatcgc ggtccacgcc caggacctcc
     1681 gcgaggttgg ctcgcatctc gtcccgccgg tcggcgatgc gcgggcgctc cgcgacgacc
     1741 acgagatcgc agcgctgtgg gctcaggccc gcagcccgga ccagccgaac cgcctcggac
     1801 agcagcatga tgccggcgat cccctcggtg gagggctcgt cgtcggggaa gtggtcgccg
     1861 atgtcgccca gggcccccgc gccgagcagc gcgtcggcca gagcgtggca gacgacgtcg
     1921 ccgtcggagt gcccggccac gccgggctca ccctcgaacc tcacccccgc cagcatgagc
     1981 ggcctcgtct cgtcgcgcgg gtgcgcatcg aagcccagac cgacgcgaag atccgtcacg
     2041 tgccctcccg gatccgaacg atcgcctcgg cccgctccag gtcctccggg gtggtgacct
     2101 tgacgttgcc gacgtcgccc gagaccaccg ccaccctgcg tccggcccgc tccagcagcg
     2161 ccgcgtcgtc ggtgaccgag aggccctccg cctcagcggc ccgatgggcc tcctccagaa
     2221 caccgcgacg gaacgcctgg ggcgtctggg ccagcgccag gtcgtggcgg gggatcgtct
     2281 cggccaccca tccgccggcg acccgcttca ccgtgtcggt cacgggcacc accggcacgg
     2341 cgccgtcggc tccctccagc gcccccagca cggccgtgaa gagctcgggg gcggccaggg
     2401 gccgggccac atcgtgacac acgaccacgt cgaagccgtc gggcagggcc tccagcccga
     2461 ggcgcacgga ggcctgccgg gagtcaccgc cctgcacggc ggcgaggaag cggggcgagc
     2521 tcgcggcctc ggactttgcc gtctcctcga gcccccgagg caccgtcacg acgaacccct
     2581 cgatcgcggg gaccgcgtcc accgtgcgga ccgcccgctc caggagcgag acgccgccca
     2641 cctccaggag cgccttgggc cgctccccgc ccagccgctc gccccggccc gcggccagga
     2701 ggatcgccgc cgcccgcatc accggtcgag gcgggcgaac aggagccgcc ccgatgccgt
     2761 ctggatcacg ttggtcaccg tcaccacgac gacggccccc aggtgggagc ggccttgctc
     2821 cacgacgacc atggtgccgt cctccaggta acccacaccc tggccctcct cgcgcccctc
     2881 ccggctgatg tggacccgca gttcctcgcc ggccatcacc ggaggccgca cggccgccgc
     2941 gagctcgttc aacgaacgga cgggcacgtt gagggcggtg gccaccttgg ccaggttcgc
     3001 gtcggaggtg atgaccacgc ctcccctgtc cttcgccagg cggaccagca ccccgtccac
     3061 gtcgccccgg accggctcgt ccacgagcac gacctcggcc gtccgctgga ggtcgtggag
     3121 gacgtcgagc ccccgccggc cgcgctgccg ccgctggggg tccgaggagt cggcgatccg
     3181 ctggagctcg tcgaggaccc ctcggtgcac gaggaagacc ccgcccagga acccggccgt
     3241 gacgacgtcc agcacccgcc cgtcgatcag cgcgctggtg tccacgacgt tgatctcgcc
     3301 cggccgcacg cccgcccccg gtggtcggag cccgaagaga gagaagaagt ccgcccactt
     3361 ggcccgcccg atccggtagc ccgcgtacga gagcgacacg gccacgaacg cgatggtggg
     3421 ccccgcggcc gcgggcggga ggcggaagat caggaacgag gcgaggacgg cgatcacgag
     3481 gcccaggatg agcccgatcg tccccgccag gatctcgccc gcggggacct cctcgaaccc
     3541 ggcctccgcc tcgtggacgg ctcggaccgt gagccgcccg atcacgccgc cgaccacgta
     3601 ccccaccatg ctcccgaggg cgatcccgat gaggagggcc gtccgtccct cgaacccgcg
     3661 ctccgcgatc tgccacccgc ccacggcgaa gacggagacg aggatgatcc tgatgagctc
     3721 gacgagggtg ccgcgggggc cgccggtcag gacgcggcgt ctgctccgat cgctcactgg
     3781 tccaccgtgc gccaccgaag gcgcggagac aagccctctc gggggcccta ccccgagccg
     3841 ttggcggagg cgacggcctt gtgcgactcc tcgagcacct tgtccaccat cgcctcggcc
     3901 ttctcctcgg gcgtgtccac cgcgaacgtg agctcggaga tcaggatctg gcgggccttg
     3961 gccagcatcc gcttctcgcc ggcggagaga cccttctcgg cctcgcggat cgagaggttc
     4021 cgcacgacct cggccacctg gtagatgtcg ccggagcgga tcttctcgat gttggtcttg
     4081 tagcgccggc tccagttcgc gggcatcctg gtctcggact tcttcagcac ctcgaggacc
     4141 ttcttgacct cgcgcttggg gacgacctcg cggagaccca cctcgtcggt gttgtccacc
     4201 ggaaccatca gggtgaggtc accgtgcgtg agcctgagga cgaagtactt cttcttctct
     4261 ccgaggagct ctcgggtcgt gagatcctcg atcacggccg cgccgtggtg gggatagatg
     4321 accttgtcac ccttgttgaa caacaagtac ccccctgagt ctgggacgga caggctctca
     4381 tgatagcagg gagtcgcctc ccccgcaaat gaaaactgca ggtcacgggg catttccggg
     4441 tcgtggaacg ccgtttcgcc tgctcaggcg gcccccctgc ccccaccctc ggcggcccat
     4501 gtgagggcgt catacaggtg tcggaccgtc ctgacgcgga cgtcggacga cctcaccccg
     4561 cgaggagcgt agacctcggt gagcccggcg gccgcggcgg cggcgaggcg cgggcccggg
     4621 ttcggggcgc cccgcaccgc ccccgtcagg ctcacctcgc cgatgaagcc gcggccgccg
     4681 ggcgggggga cgcccgtggc ggccgaggcc agcgcggcgg cgacggccag gtcgcagccg
     4741 gggtcctcga tcctgaggcc gcccgccgtg gccccgtaca gctcggtccg tcccagcctg
     4801 aggccggtgg cccggtcgac caccgcggcc acgagaccga acctcctggg atccagcccc
     4861 gtcacgtgtc gccgggggga tccctcggtg ggagcgacca gcgcctggac ctccaccgcg
     4921 acggcgcgcc tgcccgcgga tagcagcccc acagcggcgc ccggctccgc ctccctggtg
     4981 gtcagcagct cggacgggtc ggcctccacg aggccctcgg cccccatctc gaaccacgcc
     5041 agctcgccct cgggcccgaa gcggttcttc cccccggcca gcacgcgaag gccggtccgc
     5101 tggtccccgt cgaacgagca gacgacgtcc acggcgtgct ccagcgtcct ggggccggcc
     5161 acctcgccgt ccttggtgac ctgaccggcc aggatcacga tgatcccctc ggccttggcc
     5221 agccccacga ggcggtcggc gcacaggcga acctgcgtcg gtcccccagg cagcgacgtg
     5281 tggtccgggt ctctgatcgc ctggatcgag tcgaccatca gcacggccgg cagctcgacg
     5341 ctggcggcct cgagcacgtg cggcagctcg cggcccggga cgaacgagag gtccgaccgg
     5401 agggccaggc gcccggccct cgcggccacc tggccgtggg actcctcccc cgaggccagc
     5461 agcacgccgt gcccggcacc cgtcagccgg tcggcgatct ggagcagcag cgtcgacttc
     5521 ccgatgcccg gctcgcccgc cacgaggaca accgagcccg gcacgagccc tcctcccagc
     5581 acccgatcca caccctccat gccgatccgg agccgccgct ccgggtcgtt ccccctcgcc
     5641 agcagcgaca ccgggggggg cagagccgat ccgctcccgg aggaggcggg cagcgcggtc
     5701 tcgatcgtcc cccacccgcc gcaggacggg caccgcccgg cccactgacc ggaggggacg
     5761 ccgcaggccg tgcactcgaa ggtcgtgcgc ttcttcccca cgaggcaacc gtagccccag
     5821 ggtctgtcac gaacggctca ggcccgaccc cgctcgatcc gcctgacccg ccgcctcccc
     5881 gcgtcgccgc acagcacctg cagctcgacg ccgccacggc gcagccgctc cctcacgcgg
     5941 gcccggatgc ggccgctcct gatcttgcgc cgcaggcgcc gcccggggca cgccgtcgag
     6001 gcccagtcgc cgtggccgcg aatcgtgctc ggcttcacgt cgaagcgccg gcacgcccag
     6061 gccaggacgt ccacgagggc cgtgacctgc ctccccgaga gcggctgccg gtcgaagtcg
     6121 ccctcgcaca agacgaggag gtgtcccttc gggtcgtagt cggtggcggt gtcccctcgg
     6181 aacctcgggc ttctcccccg gtacacgttg ccgcctcggt cgatcagcac gtggtaggcg
     6241 atgtccggcc acccgttgcc gaagtgcgcc cgctggtgcc cgcggaaccg gccgggggcg
     6301 tcgcggttgt ccgagagcac gacggccgag tggtggacgg tgagccgccg gatccgatgc
     6361 cgcctcaggc cctcgcgggg aggagcggcg cgccacgccc ggcggcagat cacgtcgacg
     6421 cgcttggggg cggccgcgag cccggagaga gggcgcaaac ccgagaggag ggacccgagc
     6481 ccccccgcca ggaagcgccg ccggctgagc cgccgaacca tcggcgtcat gctagccgta
     6541 agacccccga aatctcctcc ctccctatac ttggccgatg cgatcgggac cggctcggct
     6601 catcatctcg ctggggatcc tggcgctctc ggcggcctcg tgcgcctccc ccgaggagcc
     6661 ggcggcgacc cggagccccg aagcggcccc cgcggccacg agcccggcgg cccccacctc
     6721 gcccccaccc accgaggagc cggccgagga tcccccggtc cccggagagc tccgcttcac
     6781 ggcgccggcg ctgggaggag gcaccatcga cggcgccgag tacgcgggca gcgacctcgt
     6841 gatctggttc tgggccccgt ggtgagggac gtgcaaccgg gaggcccccg gcgtcgctca
     6901 ggctgagcgt cggaacgacg gagtcgagtt cctggggatc gcgt
//
LOCUS       DASUTW010000214         3490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1620217, whole genome shotgun sequence.
ACCESSION   DASUTW010000214 DASUTW010000000
VERSION     DASUTW010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620217"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VF058_07345"
     CDS             <1..361
                     /locus_tag="VF058_07345"
                     /inference="COORDINATES: protein motif:HMM:NF013492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX6208155.1"
                     /translation="LAAPQIGVPLRAFCLDVSGHRRATSSHGLVVLFDPALVEVREED
                     VLREGCLSVPDLTANVRRAVRAVVRGTDPDGNRRVHQMEGFEARALLHELDHLDGLLI
                     LDRVDSAADVFPRKVYG"
     gene            413..3313
                     /locus_tag="VF058_07350"
     CDS             413..3313
                     /locus_tag="VF058_07350"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX6208156.1"
                     /translation="MTPVGSAVSSEARLCGRTRELRELVDALRRPPSLVVLEGEAGVG
                     KSRLVLELLGRPELEARRFLVGHCAAVEEPFPLGAVVEALRGLADGPSFPELSPVCGV
                     LHPLLPELSGVLPPTIEPGKDRRMRGHLVTRAVREVLGAAGELVLVLEDLHRADPQTI
                     EVLADLAASPPGRVSTLLTYRREELPEGSPLPELLAHLPPSVHRLHRTLGPLDAAGVR
                     CMIEHDLGTQGIPDGFVRGVFDSTGGLPFAVEELLGFLRDRASSLEELLAESPRRFSE
                     VPLALRDAVFDDAPIAVRDSVLGRVGRLSGRARRIVEAASIPEPPVESKLLQRVTRLG
                     EERYARAVDEAVRAGLLEEAGAGEVKFRVAIARVAVHRSLAPERHRALHLATARALAA
                     LDLRGSASIAYHYGEAESERQWARWAERAADQAVSVGDDAWAARMLDEVLSTATLSRE
                     ARVRLSVKFARAALYGLQQAEAIGVVRHIFHTETLTGPVRGELRLALARLLMEAGDAR
                     EGHREASVAASELRGRPAEAEAEAIVASPWVEGVSPGTHLAAEARAAELLPTLTDPEQ
                     RAHVEGDLMIARLLRGEPDAVEEIESLERSSLAIGVGRELQRTRISAALALMLLGAYD
                     RAAQALKEAMVVTQDPSFARLTPYVLADEVLLDFLTGRWDGLLERAQALQHGGPPQAT
                     NKARLVIALLAMARGATARAEAILGELVPRILTAGGVPLAASALSAISFARLSRGHVG
                     GALDAAREGLDLIRSKEMWPVGGEAVSFFVSAALRAGRRSEAQAAVAELSDGIRGRFA
                     PAAHAALLTARALLQGEKGASAYRRAARAWEALPRPLAVAACREGEGEALASTDGGRA
                     RAALTESLAILELVGASWYADRVRRKLRGSGVVVSRPWRGGRRGYGDELSPRELEVAR
                     LVAEGRTNRQIAESLYLSPRTVGHHVSNAMRKLGVTSRAALAVEATSGGGEE"
BASE COUNT          408 a         1290 c         1313 g          479 t
ORIGIN      
        1 cctggccgcc ccgcagatcg gagtgcccct gcgtgccttc tgcctcgacg tctcgggcca
       61 ccggagggcc acgagcagcc acggcctggt cgtcctcttc gacccggccc tggtcgaggt
      121 gcgggaggag gacgtgctgc gcgaggggtg cctctcggtc cccgacctca ccgcgaacgt
      181 gcgccgtgcg gtccgggccg tggtccgggg gaccgacccg gacgggaacc gccgggtgca
      241 ccagatggag gggttcgagg cccgggccct cctgcacgag ctcgatcact tggacgggct
      301 cctgatactc gaccgcgtcg actccgcggc ggacgtcttc cctcggaagg tctacggata
      361 ggaccgtgtt gcccggtttc cggtacgatg cccggatgcc gcggagaacg cgatgacgcc
      421 ggtcgggtcg gccgtttcct ccgaggcccg cctgtgcggg cggacacggg agctccgcga
      481 gctcgtcgac gcgctgcgtc gcccgccgtc cctcgtggtg ctcgagggcg aggccggggt
      541 ggggaagagc cggctggtgc tcgagctcct cggccgcccg gagctcgagg cgcggcgctt
      601 cctcgtgggt cactgcgcgg cggtggagga gcccttcccc ctcggggccg tcgtcgaggc
      661 gctgcgcggc ctcgccgacg gcccctcctt ccccgagctg agccccgtct gcggcgtgct
      721 tcacccgctg ctccccgagc tctccggagt tctgccgccg acgatcgaac ccggcaagga
      781 ccggcggatg cggggccacc tcgtcacccg ggccgtccgc gaggtgctgg gggcggccgg
      841 cgagctcgtg ctggtcctcg aggacctgca ccgggccgac ccccagacga tcgaggtgct
      901 ggcggacctg gccgcctccc cgccggggcg cgtgtcgacc ctgctgacct accgccgcga
      961 ggagctgccc gagggctccc ccctccccga gctcctggcc catctccctc cttctgtcca
     1021 ccggctccac cggacgctcg ggccgctcga tgcggcgggg gtgcggtgca tgatcgagca
     1081 cgacctcgga acccagggga tccccgacgg cttcgtccga ggcgtcttcg acagcacggg
     1141 cgggctcccc ttcgcggtcg aggagctcct ggggttcctg cgggaccggg cgagctccct
     1201 cgaggagctg ctggccgagt cgccccgccg cttctcggag gtcccactgg ccctgcggga
     1261 cgcggtcttc gacgacgcgc cgatcgccgt ccgggactcg gtcctcggcc gcgtcggtcg
     1321 cctctccggg cgcgcccgcc gcatcgtgga ggcggcgtcg atccccgagc cgcccgtgga
     1381 gagcaagctg ctgcagcgcg tcacgaggct gggggaggag aggtacgccc gcgcggtgga
     1441 cgaggcggtc cgagccggcc tgctcgagga ggccggcgca ggagaagtga agttccgtgt
     1501 ggcgatcgcg cgcgtggccg tccaccgttc cctggctccg gagcgtcacc gagctctgca
     1561 cctggccacc gcccgggccc tcgccgccct cgacctccgc ggctcggcga gcatcgccta
     1621 ccactacggt gaggccgagt cggagcggca gtgggcccgg tgggccgagc gggcggccga
     1681 ccaggccgtc tccgtcggcg acgacgcgtg ggcggcccgg atgctggacg aggtcctctc
     1741 gaccgcgacg ctctctcgtg aggcccgcgt ccggctgtcg gtcaagttcg cgcgcgccgc
     1801 cctctacggc ctccagcagg cggaggcgat cggcgtggtc cgccacatct tccacaccga
     1861 gaccctgacg ggcccggtgc ggggggagct ccgcctggcg ctggcccgcc tgctgatgga
     1921 ggcgggggac gcgcgagagg gccacaggga ggcgtccgtc gcggcctcgg agctgcgggg
     1981 ccggccggcc gaggccgagg ccgaggccat cgtggcgtcg ccctgggtcg agggggtatc
     2041 gcccggaacg cacctcgccg cggaggctcg ggccgccgag ctgctcccca cgctgacgga
     2101 tcccgagcag cgggcccacg tcgagggcga cctgatgatc gctcgcctgc tgaggggcga
     2161 gccggacgcc gtggaggaga tcgagagcct cgaacggagc tccctggcga tcggggtggg
     2221 gcgggagctg cagcgcaccc gcatcagcgc cgccctggcc ctgatgctcc tgggcgccta
     2281 cgaccgcgcc gcccaggccc tcaaggaggc gatggtcgtc acccaggacc cctccttcgc
     2341 ccgcctcacc ccgtacgtgc tcgccgatga ggttctcctg gacttcctga cggggcgctg
     2401 ggacggcctc cttgagcggg cgcaggcgct ccagcacggc gggcctcccc aagcgacgaa
     2461 caaggcccgc ctcgtgatcg ccctgctggc gatggcccgg ggcgctaccg ccagggccga
     2521 ggcgatcctc ggggagctgg tgcctcggat cctcacggcg ggaggagttc ctctggccgc
     2581 ctcggccctc tcggcgatct cgttcgcccg cctctcccga ggccacgtcg gcggcgcgct
     2641 cgatgccgct cgcgagggcc tggacctcat ccggtccaag gagatgtggc ccgtgggggg
     2701 cgaggccgtg tcgttcttcg tcagcgccgc gctgcgcgcc ggcaggcgga gcgaggccca
     2761 ggccgccgtg gccgagctgt cggacggcat caggggcagg ttcgccccgg ccgcccatgc
     2821 ggcgcttctc accgcccgcg ccctccttca gggggagaag ggcgcctccg cctaccggcg
     2881 cgcagcgcgg gcctgggagg ccctgccgcg tcctctggcc gtcgccgcct gcagggaggg
     2941 ggagggcgag gcgctggcct ccaccgacgg gggccgagcc cgggccgccc tgaccgagag
     3001 cctggcgatc ctggagctgg tgggggccag ctggtacgcc gatcgggtcc gccggaagct
     3061 ccgcgggtcc ggcgtcgtcg tgtcccgtcc ctggcgagga gggaggcgcg gctacggcga
     3121 cgagctctcg ccccgggagc tcgaggtggc ccggctcgtg gccgaaggtc gaaccaaccg
     3181 gcagatcgcc gagagcctgt acctgtcccc tcggacggtc gggcaccacg tctccaacgc
     3241 gatgcggaag ctgggcgtga cctcccgggc ggccctggcc gtcgaggcca cgagcggcgg
     3301 aggggaagaa taggtcatcc gcaccatcgc gagcatctcc cgcatggggc atgctgatgg
     3361 tccggcgccg acggagccga ggtgacgctg tcagcagagc gggttgggaa cgtcgcaacg
     3421 ttgctcccgg tcggcgcccg gaacgacgga gcgagggcgg gcgacggccc gccctcctcc
     3481 ctcaaacccc
//
LOCUS       DASUTW010000215         2302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_454150, whole genome shotgun sequence.
ACCESSION   DASUTW010000215 DASUTW010000000
VERSION     DASUTW010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454150"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VF058_07355"
     CDS             <1..261
                     /locus_tag="VF058_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208157.1"
                     /translation="FPTEPEAYVGVRRGGTLTVSGDAETVEVGVARFRPGDVAAELEV
                     VQDLPLEDGSATIDVEPGRYLLQVFATFEEGDGAFALGLRVL"
     gene            complement(258..1136)
                     /gene="era"
                     /locus_tag="VF058_07360"
     CDS             complement(258..1136)
                     /gene="era"
                     /locus_tag="VF058_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX6208158.1"
                     /translation="MTRSGFVAVVGRPNTGKSTLVNALVGLKVSIVSDKPQTTRGRIR
                     GVVTGEGYQVVLTDTPGFHKPRTLLGERLNEMVGESTGDVDAIVLVVDGAAGVGRGDA
                     YAAARQVASHDVLKVCAVNKVDRLSKAAVLPQLEAAAELTDFDHVMPISARTGAGVPE
                     LLGILVEALPEGPFLFPEGQATDQPMERRVAEIVREKALAATREEVPHSIATEVEELE
                     REDGLIRIHCLVLVERESQKGIVIGKGGEMLKRIGTEARRELEALLDEKVYLELRVKV
                     LHDWQRDPKALQRLGY"
     gene            complement(1133..1546)
                     /locus_tag="VF058_07365"
     CDS             complement(1133..1546)
                     /locus_tag="VF058_07365"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020442307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX6208159.1"
                     /translation="MSSLDQDRVRALLDAAREARDLAYAPYSGFRVGAAVLTADDRVV
                     RGANVENAAYPLSVCAERTAIQRAVTEPGSREVVAVAVVSSSGGETWPCGGCRQVLHE
                     FGPAMAVITEDAQGEPTSRPLAELLPEAFGPADLP"
     gene            complement(1539..>2302)
                     /locus_tag="VF058_07370"
     CDS             complement(1539..>2302)
                     /locus_tag="VF058_07370"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX6208160.1"
                     /translation="AMAEAGSEEGAIEEEEKELIHSIFEFGDTIVREVMVPRPDMVTI
                     GKGASLDDVLDLVLRHGYSRVPATSNGLDEIAGVVYAKDVLRQLHRGRRDRTVEDLMR
                     KPYFVPESKKVSELLRDMQREKYHLAMVVDEYGAVAGLVTLEDLLEEIVGEIEDEYDR
                     EEPNVEPAGEDRLRVNARLPVDELNELLQVELPNTEWDTVGGLVMGILGRVPAVGEQV
                     EFEGLRFTAERVQGRRIAKILIERLPPGPAEATAE"
BASE COUNT          303 a          860 c          789 g          350 t
ORIGIN      
        1 ttcccgaccg agccggaggc gtacgtgggg gtgcgccgcg gcgggacgct gaccgtgtcg
       61 ggagacgcag agaccgtcga ggtgggggtg gctcggttcc ggcccggcga cgtcgccgcc
      121 gagctcgagg tggtacagga cctcccgctc gaagacggct ccgccacgat cgatgtggag
      181 ccggggaggt acctgctcca ggtgttcgcc accttcgagg aaggcgacgg cgcgttcgcc
      241 ctcggccttc gggtgctcta gtagcccagc cgctggaggg ccttggggtc ccgctgccag
      301 tcgtgcagca ccttgacgcg gagctcgagg tagaccttct cgtcgaggag cgcctcgagc
      361 tcccggcggg cctccgtgcc gatccgcttc agcatctcgc cgcccttccc gatcacgatc
      421 cccttctggg actcccgctc cacgagcacg agacagtgga tccggatcag cccgtcctcg
      481 cgctccagct cctcgacctc agtggcgatc gagtgcggga cctcctcccg cgtggcggcg
      541 agcgccttct cccggacgat ctcggccacg cgtcgctcca tcggctggtc ggtggcctgg
      601 ccctcgggga agaggaacgg cccttcgggc agggcctcga cgaggatccc cagcagctcg
      661 ggcacacccg caccggtcct ggccgagatc ggcatcacgt ggtcgaagtc ggtcagctcg
      721 gcggcggctt cgagctgagg caggaccgcc gccttcgaca gccgatcgac cttgttcacg
      781 gcgcagacct tgaggacgtc gtgcgaggcc acctgacggg cggccgcgta ggcgtccccg
      841 cgccccacgc ccgccgcgcc gtccacgacg aggacgatcg cgtccacgtc gccggtcgac
      901 tcgcccacca tctcgttgag gcgctccccc agcagggtgc gaggcttgtg gaagccgggg
      961 gtgtcggtga gcacgacctg gtagccctcc ccggtcacca cgccgcggat cctgcccctg
     1021 gtcgtctgcg gcttgtcgga cacgatcgag accttcagcc ccacgagggc gttgacgagc
     1081 gtggacttgc ccgtgttcgg gcggccgacg acggccacga agccggaccg cgtcacggga
     1141 ggtcggcggg cccgaacgcc tcgggcagca gctcggcgag cggccgggag gtgggctctc
     1201 cctgggcgtc ctcggtgatc acggccatcg cggggccgaa ctcatggagg acctggcggc
     1261 atcccccgca gggccaggtc tcgcctccac tcgacgagac gacggccacg gcgaccacct
     1321 cgcggctccc cggctcggtg acggcccgct ggatcgccgt gcgctcggcg cagaccgaga
     1381 gcgggtaggc ggcgttctcc acgttcgcgc cgcgcacgac gcggtcgtcg gcggtgagga
     1441 cggccgcgcc cacccggaac cccgagtagg gcgcgtaggc caggtccctg gcctcccgcg
     1501 cggcgtcgag cagggcccgg acccggtcct ggtcgaggct actcagcggt ggcctccgcc
     1561 ggtcccgggg gcaggcgctc gatcaggatc ttggcgatgc gccgcccctg cacgcgctcg
     1621 gccgtgaagc gcaggccctc gaactcgacc tgctccccca ccgccggcac gcggcccagg
     1681 atgcccatga cgagcccgcc caccgtgtcc cactcggtgt tcgggagctc cacctggagg
     1741 agctcgttca gctcgtccac ggggaggcgc gcgttcacgc ggaggcggtc ctcgccggcc
     1801 ggctcgacgt tcggctcctc gcggtcgtac tcgtcctcga tctcaccgac gatctcctcg
     1861 agcaggtcct ccagcgtgac gagcccggcc acggccccgt actcgtccac caccatcgcc
     1921 aggtggtact tctcgcgctg catgtctcgg aggagctcgg agaccttctt cgactcgggg
     1981 acgaagtacg gcttgcgcat caggtcctcc accgtgcggt cccggcggcc ccggtggagc
     2041 tgacggagga cgtccttggc gtagaccacg cccgcgatct cgtcgagccc gttgctcgtc
     2101 gccgggaccc gcgagtaccc gtgccgcagg acgaggtcga ggacgtcgtc cagcgaggcc
     2161 cccttcccga tcgtgaccat gtcgggccgc gggaccatca cctcgcgcac gatcgtgtcg
     2221 ccgaactcga agatcgagtg gatcagctcc ttctcctcct cctcgatcgc gccctcctcg
     2281 ctgccggcct cggccatcgc cc
//
LOCUS       DASUTW010000216         8639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1491256, whole genome shotgun sequence.
ACCESSION   DASUTW010000216 DASUTW010000000
VERSION     DASUTW010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8639
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491256"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1480)
                     /locus_tag="VF058_07375"
     CDS             complement(50..1480)
                     /locus_tag="VF058_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007540021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6208161.1"
                     /translation="MVTRRELQLAALEGGQVGLSPQQLQDLDGRLQGDLLGPGDPGWD
                     EAVETWNAMVAKTPALVARPLSPADVAAAVSFARDHGVLLSVKGGGHNIAGTSMAEDG
                     LTLDMSGMREVSVDPGARLAHAGPGCLLKDVDRATQEHGLAAPLGFVSETGMAGLTLG
                     GGWGYLTRRFGWTVDTLEEVEIVTADGEIRVANREEHPDLFWAVRGGGGNFGVVTRFT
                     FRLHEVGPTITGGMRVWSADRADEVLATYAELTASAPPELTAAAIVRLAPPAPFLPEE
                     WHGKPVANIVVCYSGGNAESDLAPVRDLGDPVADLITEKPYVEQQSMVDALEPKGLSR
                     YWKTEYLAGIDDEYLDVFREGALDVTSPLSQSVIFHLGGALNEREPDDGAVGNREARF
                     ISGFAGTWRPDTPADPHIDWVRRSWERIRPYSTGGNYVNFQVADDDSGRTAEAYVGNY
                     ERLQRVKAEYDPENLFRVNRNIPPAA"
     gene            complement(1590..1946)
                     /locus_tag="VF058_07380"
     CDS             complement(1590..1946)
                     /locus_tag="VF058_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208162.1"
                     /translation="MALAVLSAGYMVMDGTRALVRGDYLTPSSGAHAGRLGPWAGIVR
                     ALGIEPRSMGMKAFFVIYGTVWLAVTVTFALGVNWSWLAMLALAIGSLWYLIPGTLLS
                     AVIIALLLIPSVREVF"
     gene            2075..2359
                     /locus_tag="VF058_07385"
     CDS             2075..2359
                     /locus_tag="VF058_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208163.1"
                     /translation="MRKRTKRLGLAIALAGALSIIPASGAMAHVHGITPLRCVGVADD
                     GANRTNTTPAAAANGGPISGLLPSAELGSPGNSHLGFGDGGFDTPACPAP"
     gene            complement(2499..4469)
                     /locus_tag="VF058_07390"
     CDS             complement(2499..4469)
                     /locus_tag="VF058_07390"
                     /inference="COORDINATES: protein motif:HMM:NF014301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HEX6208164.1"
                     /translation="MSVSRSRWAWILALTMMFSMLAGIAAADHPDPGGGQPEHDALEA
                     PGDPSDPLEELDAAFSDVVSGGPSAKVIKNLQLRGRGERLDANATTDVWAHGDYAYTG
                     TFNDPCGGDPEAGVWVWNVEDRDNPVFVDVIESPVGSRSNDVKVATLNSGDILVHSNE
                     SCAGGPGGFEIYNVDDPTNPVHLASVQTDDVNAFLRSAFGFVDFGVHNLFLFSQGDRD
                     YVAAVVESEFGNFQVFDITDPGSPTLAGFWGAEELRMAELGEDPSTVPDLGAAEFGLI
                     LELDAYLISGFGESQNRFLHDITISADGTQAYLSNWDAGLVLLDISDVSDPTVVSVAL
                     DPANGSRDGEVNSHAAWPSEDGSIVVETEEDFNAWVTVQPLDNFTFGEHPWNTIPGVG
                     VSTSAGDDFQANQTGNMVTVTANSVTVNSGPLAPNVYPATEGSGNQPKLANTGPLEAE
                     AVWVGRACDTDRFSIGPPTDQPGVVDPLLNDPAGKIAVVRRGGCSFSSKMGHVADAGA
                     IGVIVANNLTNDSPWGGVRIWDYSDPSNPELASIFDTTCSASPEPIPECDPFGTYSVH
                     NVIVESRGNKTFAYISWYWDGMLVLDVTDPYNPVEVARYFDNSPAFLESNGGNPHDFW
                     GVYKERRSPWIYGSDRNGGLYVFKEQGSGSGK"
     gene            complement(4658..6676)
                     /locus_tag="VF058_07395"
     CDS             complement(4658..6676)
                     /locus_tag="VF058_07395"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208165.1"
                     /translation="MKRHLILAMAFVLVTALGSGVAATPSPDRPEGGGPGDHAVDDGY
                     VDTPLEVHRAHGHDEQHGTMEGHIPPTQENVDVVGIWTAPRTNKKPGRITDVWSLGNY
                     AYLGTFAPPCNGLGVNVVDISDPTNPTKAAFIPSRPGTRVNDVKVIHLETSAFTGDVL
                     LHSNENCSAHPQRVGGISLWDVTDPTNAQPLAQGVGDTNGGTLPRARQVHNIFAWQDG
                     DEAYAAIVDDEELLDVDILEITDPTNPEHVAETGLPDWPNVNVDAFGGDAFVHDIWFD
                     IVDGIPQLLLSYWDAGWIRLDVTDPENPTIIEPPGDSDYPFPDPLASAAAGQPLLAEG
                     NAHAAVWDWTGRLILAGDEDQTPFRIENFEITTGSNAGPFGAGEFGFTKSVSSFPGGE
                     VSGPTVYGGSACPGQDLDGDGTDDRVQVPDASILDPQVGPDDERVLVTTRGTCFFSDK
                     IRTGEEKGYDVVIIGNHHIGAAGGAAPDAFFCGGQGSPVLGTAAGLCIGHRAMHLIFN
                     DPPEYTPETSDTSPDMPATGTLGERVQASAIFDGRGPFHLLDHDTMEEIDTYAPSEVH
                     DPAFAQGFGELTMHNVEADPTRDLAYIAWYALGFRVLDFSECDTDASDGSFDNLDDPG
                     CVQEVGNFIDQGGNDFWGIHQALDHSSDPDLILLSDRDRGLYIVKFTG"
     gene            complement(6913..7896)
                     /locus_tag="VF058_07400"
     CDS             complement(6913..7896)
                     /locus_tag="VF058_07400"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX6208166.1"
                     /translation="MTRLVVLLAGLAVFMVTPRVLERLRSAPPRWRARLALLSVLGMA
                     ASSVSLLLVILFPEALLASSMTDMWHSEAIHELVRRPPLRAASYTAGGILGFVLVRFL
                     WGLTSGLRSTSRARIRDRRPERTDGGASIYIVPMNAPMAYSLGRGQVVVSQGLFGALD
                     RSEVRAVLLHEEGHVRSRDHRKLLLVRAIRHAIGMLPPVGAAAEAMEQAMEEAADEHA
                     AARLGDPAVVASALSKAALSGLRTPAGAMGLTSEIDVLGRVRRLLEPPLVPQWMPWAC
                     LAVAGALFAFLALTQVMVGIAIVAATHHLLNAGALAIAPVPSVRLGLRAGT"
     gene            complement(7893..8276)
                     /locus_tag="VF058_07405"
     CDS             complement(7893..8276)
                     /locus_tag="VF058_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018504947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX6208167.1"
                     /translation="MRHGMRTLGELEARVMRVMWRASAPMTVRDTLAELAPEREFAYT
                     TVMTILDRLWRKGFLERTMRGRAYEYHAVVSEGEYTAELMHQLLAGASDRRAALAHFV
                     GGMKAPDEEELRRLADEASRRKRSR"
BASE COUNT         1284 a         3033 c         2954 g         1368 t
ORIGIN      
        1 cggatccatc cggcctcggc cgcgagatga cctggcgccg cccgaccttc tacgccgcgg
       61 gcgggatgtt gcggttcacc cggaacaggt tctccgggtc gtactccgcc ttgacccgct
      121 ggagccgctc gtagttcccg acgtaggcct ccgccgtccg ccctgagtcg tcatcggcga
      181 cctggaagtt cacgtagttg ccccccgtcg agtacgggcg gatcctctcc caggaccgcc
      241 gcacccagtc gatgtggggg tcggccggcg tgtccggccg ccacgtcccc gcgaacccgc
      301 tgatgaaccg cgcctcgcgg ttcccgacgg cgccgtcgtc cggctcgcgc tcgttgagcg
      361 cgccgcccag gtggaagatg accgactgcg agagcggcga tgtcacatcg agcgccccct
      421 cccggaagac gtcgaggtac tcgtcgtcga tccccgccag gtactcggtc ttccagtacc
      481 ggctgagtcc cttgggctcc agggcgtcga ccatggactg ctgctcgacg tagggcttct
      541 cggtgatcag gtcggcgacc gggtcgccga ggtcacgcac gggagcgagg tccgattcgg
      601 cgtttccgcc gctgtagcag accacgatgt tggcgaccgg cttcccgtgc cactcctcgg
      661 ggaggaacgg ggccggcggc gccagccgca cgatcgcggc cgcggtgagc tcgggcgggg
      721 ccgaggcggt cagctccgcg tacgtggcga ggacctcgtc ggcccgatcg gcgctccaca
      781 ccctcatccc gccggtgatc gtgggcccca cttcgtggag gcggaacgtg aagcgcgtga
      841 ccacgccgaa gttccctcct ccgccgcgga ccgcccagaa gaggtccggg tgctcctcgc
      901 ggttcgccac gcggatctct ccgtccgccg tgacgatctc gacctcctcg agggtgtcca
      961 ccgtccaccc gaagcggcgc gtcagatagc cccacccgcc gccgagcgtc agcccggcca
     1021 tgccggtctc ggagacgaac cccagcggag cggccagccc gtgctcctgc gtcgcgcggt
     1081 ccacgtcctt caggaggcag ccgggcccgg cgtgcgcgag ccgcgcgccc gggtccacgc
     1141 tcacctcgcg catcccggac atgtcgaggg tgagcccgtc ctcggccatg gaggtcccgg
     1201 cgatgttgtg gccgccgccc ttcacgctca gcagcacccc gtggtcccga gcgaagctca
     1261 ccgccgcggc cacgtcggcc ggcgacaggg gacgggccac gagcgcgggc gtcttggcca
     1321 ccatcgcgtt ccacgtctct acggcctcgt cccagcccgg gtcgccgggg ccgagcaggt
     1381 cgccctggag gcgaccatcg aggtcctgga gctgctgggg tgagagaccg acctggcctc
     1441 cctcgagtgc ggcgagctgc agctctcttc tcgtcaccat gcctgcctcc tctccctcgg
     1501 attccgaccg ggttgaacga tagggaccac cctatcgggg gcggtacccg agcagagttc
     1561 ctggaaacac gggatcgtct gcaggagatc tagaagacct ctcgcacgga ggggatgagc
     1621 agcagcgcga tgatgactgc gctgagcagc gtgcctggga tgaggtacca gagcgagccg
     1681 atcgccagcg ccagcatcgc gagccatgac caattgacgc cgagggcgaa cgtcaccgtg
     1741 accgcgagcc acaccgtccc gtagatgacg aagaacgcct tcatccccat ggatcgcggc
     1801 tcgatgccga gggctcgcac gatcccggcc cacgggccga gccgcccggc atgcgcaccg
     1861 gaggaaggcg tcaggtaatc gcctcgcacg agcgcgcgcg tgccgtccat caccatgtag
     1921 ccggcgctga gcacggccag cgccacgatg atccactgga gccacgccat ctgagtcctt
     1981 cgctgcggat gatgccacgt gggggaggag gaatccggac ccttgccggc ggagtagtac
     2041 ccgtgacggc actcgacaca aggaggtggt gcacgtgcgg aagcgaacga agcggctggg
     2101 cctggcgatc gcgctcgcgg gggcgctctc gatcatcccc gcgagcggcg ccatggccca
     2161 cgtccacggg atcacgccgc tccgctgtgt gggcgtggcg gacgatgggg cgaaccggac
     2221 caacacgacg ccggcggccg ccgcgaacgg cgggccgatc tcgggcttgc ttccgagcgc
     2281 cgagctgggg agcccaggca actcgcacct cgggttcggc gacgggggct tcgacacgcc
     2341 cgcctgcccc gcgccgtagg gagctcgagg cttcctcgcg atcgataggg aggcgggatc
     2401 tcgcagctgg cgcgcggcgc cgaggggtcc tcggtccgga tagggggcgg ggggcggccg
     2461 gggtctcgac ggccgccccc caccggggaa ctccggttct acttaccgga tccggagccc
     2521 tgctccttga acacgtacag cccgccgttc cggtccgagc cgtagatcca cggcgagcgc
     2581 cgttccttgt acacgcccca gaagtcgtgc gggttccctc cgttcgactc caggaacgcg
     2641 gggctgttgt cgaagtacct cgccacctcg acggggttgt acggatcggt cacgtcgagg
     2701 accaacatcc cgtcccagta ccacgagatg taggcgaagg tcttgttgcc cctgctctcg
     2761 acgatcacgt tgtgcaccga gtaggtcccg aacgggtcgc actccgggat cggctcgggc
     2821 gatgcgctgc acgtcgtgtc gaagatcgac gccagctcgg ggttggaggg atccgagtag
     2881 tcccagatcc gcaccccgcc ccacggcgag tcgttcgtga ggttgttggc gacgatgacc
     2941 ccgatcgctc ccgcgtccgc cacgtgcccc atcttgctgg agaacgagca gccaccacga
     3001 cgcacgaccg cgatcttgcc cgccgggtcg ttcaggagcg gatccaccac gccgggctga
     3061 tccgttgggg gcccgatcga gaagcggtcc gtgtcgcagg cccgtccgac ccacacggcc
     3121 tccgcctcga gcggcccggt gtttgccagc ttgggctggt tcccagaccc ctccgttgcg
     3181 gggtagacgt tcggggcgag cggcccgctg ttcacggtca cgctgttcgc cgtgaccgtg
     3241 accatgttcc ccgtctggtt ggcctggaag tcgtcgcccg ccgaggtgct gacacccacg
     3301 ccggggatcg tgttccaggg gtgctcgccg aacgtgaagt tgtccagcgg ctggaccgtg
     3361 acccaggcgt tgaagtcctc ctcggtctcg accacgatcg acccgtcctc gctcggccag
     3421 gccgcgtggc tgttcacctc gccgtcccgc gaaccgttgg cggggtccag ggcgaccgat
     3481 accacggtcg gatccgatac atcgctgatg tcgagcagga cgagcccggc gtcccagttc
     3541 gagaggtacg cctgcgtccc gtccgcggag atcgtgatgt cgtggaggaa gcggttctgc
     3601 gattccccga acccgctgat caggtacgcg tcgagctcca ggatcaggcc gaactcggcc
     3661 gcgcccaggt cgggcacggt cgaggggtcc tctcccagct cggccatccg cagctcctcg
     3721 gcgccccaga acccggccag cgtcgggctc cccgggtcgg tgatgtcgaa gacctggaag
     3781 ttcccgaact ccgactccac cacggccgcc acgtagtcgc ggtcgccctg cgagaacagg
     3841 aagaggttgt gcacaccgaa gtccacgaac ccgaacgcgc ttcgcaggaa cgcgttgacg
     3901 tcgtcggtct ggacggaggc aaggtgcacc gggttggtgg ggtcgtccac gttgtagatc
     3961 tcgaagccgc ccgggcctcc tgcgcagctc tcgttcgagt gaacgaggat gtcgccggag
     4021 ttcaaggtcg ccaccttcac gtcgttggag cggctgccca cgggcgactc gatgacgtcc
     4081 acgaacacgg ggttgtccct gtcctccacg ttccagaccc acacgccggc ctcggggtca
     4141 ccgccgcacg ggtcgttgaa ggtccccgtg tacgcgtagt ccccatgcgc ccacacgtcg
     4201 gtggtggcgt tggcatcgag gcgctccccg cgccctcgaa gctgcaggtt cttgatcacc
     4261 ttcgcgctcg ggccgccgct caccacatcc gaaaacgcgg cgtccagctc ctcgagggga
     4321 tcactggggt cgcccggtgc ctccagggcg tcgtgctccg gctgcccgcc cccagggtcg
     4381 ggatggtccg ctgccgcaat tccggcgagc atcgagaaca tcatggtgag cgccaggatc
     4441 cacgcccacc tcgatcgcga taccgacatc tctcgcctcc ttctgggtcc ccgagcacca
     4501 tggcgacggg caaccgcgtc gtcatccttg ggcggactac gaaggctagt actaccgccg
     4561 ctgggaggtc aaaccggacg agcgggggac cgggggcccg gaaggcaaag gggggcgggg
     4621 tggtcccgcc cccctttgcc ttcggcctgt tccgagctca gcctgtgaac ttcacgatgt
     4681 acaggccgcg gtcccggtcg ctcagcagga tcaggtcggg atcgctggag tggtcgagcg
     4741 cctggtggat accccagaag tcgttcccgc cctggtcgat gaagttcccg acctcctgca
     4801 cgcatcccgg gtcgtccagg ttgtcgaagc tcccatcgga tgcgtccgtg tcgcactcgg
     4861 agaagtcgag cacacggaag cccagcgcgt accaggcgat gtacgcgagg tctcgcgtcg
     4921 ggtccgcctc cacgttgtgc atcgtgagct ccccgaaccc ctgagcgaac gccgggtcgt
     4981 ggacctcgga aggcgcgtag gtgtcgatct cctccatcgt gtcgtgatcg agcaggtgga
     5041 acgggccacg cccgtcgaag atcgccgagg cctgcacccg ctcgccgagc gtgccggtcg
     5101 ccggcatgtc gggcgacgtg tcgctggtct cgggcgtgta ctccggcggg tcgttgaaga
     5161 tcaggtgcat ggcgcggtgg ccgatgcaga gccccgccgc cgtgccgaga accgggctgc
     5221 cctggccgcc gcagaagaag gcgtccggag ctgcgccacc ggcggcaccg atgtggtggt
     5281 tgccgatgat caccacgtcg tagcccttct cctcaccggt ccggatcttg tcggagaaga
     5341 agcaggttcc gcgcgtcgtc accagcaccc gctcgtcgtc cggccccacc tgggggtcga
     5401 ggatcgaggc gtcgggcacc tgcacgcgat cgtcggtccc atccccgtcc aggtcctggc
     5461 cggggcatgc gctgccgccg tagaccgtcg gacccgacac ctctccgcct gggaacgagg
     5521 agacagactt cgtgaagccg aactctcccg ctccgaaagg cccggcgttc gaccccgtcg
     5581 tgatctcgaa gttctcgatc cggaagggcg tctggtcctc atctcccgcc aggatcagcc
     5641 tgccggtcca gtcccacacc gccgcatggg cgttgccttc cgccagcaag ggttgccctg
     5701 cggcggcgct cgccagtggg tccgggaagg ggtagtcgga gtcacccggc ggctcgatga
     5761 tcgttgggtt ctccgggtcc gtcacgtcga gccggatcca tccagcgtcc cagtaggaaa
     5821 ggaggagctg tgggatgccg tcgacgatgt cgaaccagat gtcgtggacg aaggcgtccc
     5881 ctccgaaggc gtcgacgttc acgttcggcc agtcgggaag ccccgtctcc gccacgtgct
     5941 cggggttcgt cggatcggtg atctcgagga tgtccacgtc gaggagctcc tcgtcatcca
     6001 cgatcgcggc gtacgcttcg tcaccgtcct gccaggcgaa gatgttgtgc acctggcggg
     6061 cccgcgggag cgtgccgccg ttagtgtccc ccacgccctg ggccagcggc tgggcgttcg
     6121 tggggtcggt gacgtcccag agagagatgc ctccgacccg ctgcgggtga gcgctacagt
     6181 tctcgttcga gtgcagcagc acgtcaccgg tgaaggcgct cgtctcgagg tggatcacct
     6241 tgacgtcatt gacccgcgtg ccggggcgag acgggatgaa cgcggccttg gtggggttcg
     6301 tcgggtcgga gatatcgacg acgttgactc cgagcccgtt gcacggtggg gcgaaggtcc
     6361 ccaggtacgc gtagttgccg agcgaccata cgtcggtgat ccgccctggc ttcttgttgg
     6421 ttcgaggcgc ggtccatatg cccaccacgt ccacgttctc ttgggtcgga gggatgtggc
     6481 cctccatggt gccgtgttgc tcgtcgtgac cgtgggctct gtgtacttcg aggggggtat
     6541 cgacgtatcc gtcgtcgacc gcatggtccc cgggtcctcc accctccgga cggtccggtg
     6601 agggggtcgc ggcaacgccg ctccccagcg cggtgacgag gacgaacgcc atcgccagga
     6661 tgagatgtcg tttcatgctc gggctccttc cggtcgtggt acgggtgcga aaactcgtga
     6721 cgtgagcacg aatcgtttgg gtcggatgct cacctcctcg gggttggggg tgagagatgg
     6781 cgcgcgggcg cttcggtaag cgacttcctg gcgtcgggct cctttcgccg gaacatatgc
     6841 cgaccctcac ggccgatcgc gaaagcaact aggaagcgta gtactaggcc ggttcgtagg
     6901 tcaaaccggc gttcacgtcc cggcgcgcag gccgagtctg accgacggca cgggggcgat
     6961 cgcgagcgcg cccgcgttca acaggtgatg ggtggccgcc acgatggcga ttcccaccat
     7021 cacctgcgtc agcgcgagga acgcgaacag ggcgcccgcg accgccaggc acgcccacgg
     7081 catccactgc gggacgaggg gcggctccag gagccggcgc acccgtccca ggacgtcgat
     7141 ctccgaggtg agccccatcg cgccggcggg cgtccgcagc ccagagagcg ccgccttgga
     7201 gagagcgctc gccacgacgg cggggtcgcc gaggcgcgcc gccgcgtgct cgtcggccgc
     7261 ctcctccatc gcctgctcca tggcctcggc cgccgccccg accggtggga gcatcccgat
     7321 cgcgtgtcgg atcgcccgga cgaggagcag cttccggtga tcccgcgagc ggacgtggcc
     7381 ctcctcgtgg agcaggacgg cccggacctc ggatcggtcc agggcgccga agagcccctg
     7441 cgacacgacg acctggccac gccccagcga gtacgccatc ggcgcgttca tcggcacgat
     7501 gtagatcgac gccccgccgt cggttcgctc gggacggcga tcccggatgc gcgcccgcga
     7561 cgtgcttcgg agcccgctgg tcaggcccca gaggaaccgg acgagcacga acccgaggat
     7621 gccgcccgcg gtgtacgagg ccgcccggag cggaggtcgc ctcacgagct cgtggatcgc
     7681 ctcggagtgc cacatgtcgg tcatgctcga ggcgaggagg gcctcgggga acaggatcac
     7741 caggagcagg ctgacggacg aggccgccat ccccagcacg ctcaggaggg cgagccgagc
     7801 gcgccaccga ggcggcgcgc tccggaggcg ctcgaggacg cggggggtca ccatgaatac
     7861 cgccaacccg gccaagagga cgacgaggcg ggtcacctcg atcgcttcct cctcgacgcc
     7921 tcgtcggcga gcctccggag ctcctcctcg tcgggcgcct tcatgcctcc cacgaagtgg
     7981 gcgagcgcgg ctcgccgatc ggacgccccc gcgagcagct ggtgcatcag ctccgccgtg
     8041 tactcgccct ccgagacgac cgcgtggtac tcgtacgcgc ggccgcgcat ggtccgctcg
     8101 aggaacccct tccgccagag ccgatcgagg atcgtcatga cggtggtgta cgcgaactcc
     8161 cgctcggggg cgagctcggc caaggtgtcg cgaacggtca tgggtgccga ggcgcgccac
     8221 atgacccgca tgacgcgcgc ctccagctca ccgagggtcc tcatgccgtg gcgcatccgc
     8281 gagattctat ccccgccttc gagccgtggg cgaccgatcg tgcggaggct ccgtgtacgc
     8341 gcacccgccg tgacccacag gcaactttca tcccactttc atccgctttg ccagggtggg
     8401 ggcggcctcg cagggaggcc cggacgcccg ccgccccggg gtccggccgg cgaccccgag
     8461 gagggatgcc ccatggatcc tcgcgccacc cgcgcgcgac acctggcgac gttggcggcg
     8521 gcggcgctgc tcgccctgag cttcgccacg cccgcccacg cagcgccgcc gccgctcagc
     8581 ctgatcggtc agggggtcgg ccccctggtc ggcggcgacg tcgtcgtcga gcgaaccga
//
LOCUS       DASUTW010000217         5627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_714348, whole genome shotgun sequence.
ACCESSION   DASUTW010000217 DASUTW010000000
VERSION     DASUTW010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714348"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..825
                     /locus_tag="VF058_07410"
     CDS             127..825
                     /locus_tag="VF058_07410"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX6208168.1"
                     /translation="MLYVGTSGFAYDEWRGSFYPEDLKRDAYLEHYTTRFPAVEINYT
                     FRRFPSPKTLERWRGQSADGFRFVLKAHGRITHTRRLRDTDEDVRSFLELARLLGDRL
                     GPILFQCPPNLPYDESLLAAFLGYLPPDVQAAFEFRHPSWEEAKPLLASQGAAWVDSE
                     TDERAVDGVAAGPFAYLRLRKDDYSEEELEAWADRIAEAVGAGRDVYCFLKHEQEAFR
                     RADRLRDLVGARVG"
     gene            complement(829..1632)
                     /locus_tag="VF058_07415"
     CDS             complement(829..1632)
                     /locus_tag="VF058_07415"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HEX6208169.1"
                     /translation="MSKPHSGYLGEDWEPAEHPREVLIRALLDGGVAGVNSHDRPNVL
                     WKIQRLCDGDPDLQFGLTGVDGMAPGDVLDLMAREGGFDGDPSLKHGLIMVDPELVLR
                     GCEAIGDRLALAAERGESVLLATGHPSGLPLLYQATGRLLTEHGAKLLRPLEGFTWKE
                     RGRKRQIRYMGGVAVYTDRASALHTHSPEPMRRMLEEVTPDLVFADHGFAGAAIEAGI
                     DTVSIADVNDPALVVAWRQGRGGPVVVMDDNVQPEAYWPCFQAIASRFG"
     gene            1661..2428
                     /locus_tag="VF058_07420"
     CDS             1661..2428
                     /locus_tag="VF058_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6208170.1"
                     /translation="MPGLDGRVALVTGGSKGIGKAIALALAEDGADVALAARGEEDLA
                     AAAKEVEALGRRALPVPTDVTDPEQVGTLVRRTIDELGGLDVLVNNAGAAPFLSTVES
                     IRPEGFEKYFRVNFHSAFYATQAAASHLLEQGSGSVVNVASVAAFIASPGLTYYAGAK
                     AALIAFTKTVAQEWAPHGIRVNAVAPGWIETDLNAYSRQDTGFVESTRAQIPMGRWGT
                     AEEVAAVVRFLASDAASFMTGSVVVVDGGQTLTALGG"
     gene            2428..3183
                     /locus_tag="VF058_07425"
     CDS             2428..3183
                     /locus_tag="VF058_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6208171.1"
                     /translation="MTTKVAVVTGASSGIGEATARALASEGLRLVLAARREDRLRALA
                     EELGGEVLPVRCDVTLKKDLRALVAATEERFGRCDVLINGAGVPGRGRFADVDLGQLE
                     AVTFTNYLGLLRATKLFLPMLLEARGHVVNVASLAGLFALPGAEAYAASKHAVVALSE
                     SLSLSLGDALKVTAVCPGFVNTEGFPQPEKPRPVTVPLEVVAEGIVDVLRRGRTGMVA
                     IPAWPGPVSAVQRLIPRLYRRALQRGIGRFRPR"
     gene            complement(3176..3850)
                     /locus_tag="VF058_07430"
     CDS             complement(3176..3850)
                     /locus_tag="VF058_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020521792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX6208172.1"
                     /translation="MRIGVLGTGSVGRALGGKLAELGHEVMLGTRDVEALTARPAGER
                     ESESFSEWMERTPGATVGTFAEAAAHGEMLFNCTRGEGSLDALASADDRQVGGKVLVD
                     VSNPLEFEEGAMKLFVPSGDSLAERIQRQHPHAKVVKALNTVNARVMVDPAGVGGGDH
                     TIFVCGDDEEAKAAVGEILRSFGWRHIMDLGDLSAARGMEAYLLLWIRMMGAMGSPAL
                     NVKVVT"
     gene            complement(3862..5004)
                     /locus_tag="VF058_07435"
     CDS             complement(3862..5004)
                     /locus_tag="VF058_07435"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX6208173.1"
                     /translation="MRIGILTGGGDVPGLNACIKAVVERVADQGHEVVGIRRGWDGLL
                     STNLDEPNTVPSNLRPLDRAAVRTIDRTGGTVLHTSRTNPGRVRAKDVPSFLEPSGEE
                     PFDFTPHVLRVLESQTIDVLIPIGGDDTLSYALRMHEEGVPVIAVPKTMDNDVHGTDY
                     CIGFSTAVTRGVQFIHNLRTSTGSHERIAVVELFGRYSGETSLITAYLAGVDRAAISE
                     VPVDVERLAGLLVEDKRTNPSNYAMLTISEGAVIEGGDLMLSGEEDAFGHRKLGGIGS
                     QVAQLIKRITGEGIVEQQLGYLVRSGSPDSLDLMVATNYAVMAADLALEGSSGRMVAL
                     RGGTYTNVPISVTREGVKRVDVDELYDVEQYRPKVRHVGGKPMFLY"
     gene            complement(5045..5488)
                     /locus_tag="VF058_07440"
     CDS             complement(5045..5488)
                     /locus_tag="VF058_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6208174.1"
                     /translation="MSGFSIRDALPEDADDVARLMAFVGGTIPPGDHRARPGLESFLS
                     QDGAVLVAESEGRVVGACTLLFTRFNPMDATPGAWLDGLAVDESHRRRGIGRALMVEA
                     RRRAEGAGCDSIVLHTHEDQGAALDLYGSLALKRHGLLMVWPLSR"
BASE COUNT          773 a         1995 c         2038 g          821 t
ORIGIN      
        1 cgcatgagct gggcgacctc ttgcacggtc acgaagtgag cttctcggaa cgatctgtca
       61 ggggccacgc tggcgcgatt gtagcccagc cctcgctcca cgggccagcc gtggttggat
      121 accggcatgc tctacgtcgg gacgtccggg ttcgcctacg acgagtggcg ggggtccttc
      181 tacccggagg acctgaagcg cgacgcatac ctcgagcact acacgacgcg gttcccggcg
      241 gtggagatca actacacgtt ccggaggttc ccgtcgccga agacgctgga gaggtggcgg
      301 gggcagagcg cggacgggtt ccggttcgtg ctgaaggcgc acggccggat cacgcacacg
      361 cggcggctgc gcgacaccga cgaggacgtg cgctcgttcc tggagctggc caggctgctc
      421 ggggaccggc tggggccgat cctgttccag tgcccgccga acctcccgta cgacgagtcg
      481 ctgctggcgg cgttcctcgg gtacctcccc ccggacgtcc aggcggcgtt cgagttccgg
      541 cacccctcgt gggaggaagc gaagccgctg ctggcgtccc agggcgcggc gtgggtggac
      601 tcggagaccg acgagagggc cgtggacggg gtcgcggccg gaccgttcgc ctacctgcgc
      661 cttcggaagg acgactactc ggaggaggag ctggaggcgt gggcggaccg gatcgcggag
      721 gcggtcggcg cggggcggga cgtgtactgc ttcctcaagc acgagcagga ggccttccgc
      781 cgcgccgacc gcctccgcga cctcgtcggc gcgcgcgtgg gctgacgcct acccgaaccg
      841 cgaggcgatg gcctggaagc agggccagta cgcctcgggc tggacgttgt cgtccatcac
      901 caccaccggc ccccctctcc cctggcgcca cgccacgacg agcgccgggt cgttcacgtc
      961 ggcgatcgag accgtgtcga tccccgcctc gatcgcggcc cccgcgaacc cgtggtcggc
     1021 gaagaccagg tcgggcgtga cctcctcgag catccgccgc atcggctcgg ggctgtgcgt
     1081 gtggagcgcg gacgcccggt ccgtgtacac ggccacgccg cccatgtacc ggatctggcg
     1141 cttgcggccc cgctccttcc aggtgaagcc ctccagcggg cgcagcagct tggccccgtg
     1201 ctcggtgagc agccgaccgg tggcctggta gaggaggggc agcccggagg ggtgccccgt
     1261 ggccagcagc accgactccc cccgctcggc ggccagcgcc aggcggtcgc cgatcgcctc
     1321 gcatccgcgc aggacgagct cggggtccac catgatcagc ccgtgcttca ggctcgggtc
     1381 gccgtcgaag cctccctccc tcgccatgag gtcgagcacg tcgccggggg ccatgccgtc
     1441 cacacccgtc agcccgaact gcagatccgg gtcgccgtcg cagagacgct ggatcttcca
     1501 gagcacgttg ggccggtcgt gcgagttcac ccccgccacc ccgccgtcga ggagcgcccg
     1561 gatcaggacc tcgcgcggat gctcggcggg ctcccagtct tcgcccaggt agccggagtg
     1621 tggtttgctc atcgggccat cgtatacgga ggtctcgcgg atgcctgggc tggacggcag
     1681 ggtcgccctc gtgacgggcg gctcgaaggg gatcgggaag gccatcgcgc tggcgctggc
     1741 cgaggacggc gccgacgtgg cgctcgccgc ccggggtgag gaggacctcg cagcggcggc
     1801 gaaggaggtc gaggcgctgg gccgtcgcgc cctccccgtc cccaccgacg tcaccgatcc
     1861 cgagcaggtg gggacactcg tgcgtcgaac gatcgacgag ctgggcggcc tcgacgtcct
     1921 ggtgaacaac gcgggagcgg ctccgttcct gtccacggtg gagtcgatcc ggcccgaagg
     1981 gttcgagaag tacttccgcg tgaacttcca ttcggcgttc tacgccaccc aggccgcggc
     2041 gtcgcacctg ctggagcagg gatcgggctc ggtggtcaac gtggcctcgg tggccgcgtt
     2101 catcgccagc cccgggctga cctactacgc gggggcgaag gcggcgctga tcgccttcac
     2161 gaagacggtg gcccaggagt gggcgcccca cgggatcagg gtgaacgcgg tggccccagg
     2221 gtggatcgag accgacctca acgcgtactc gaggcaggac accggcttcg tcgagagcac
     2281 gcgggcccag atcccgatgg ggcgctgggg gacggccgag gaggtggcgg cggtggtgcg
     2341 gttcctggcc tcggacgcgg cctcgttcat gaccgggtcg gtggtggtcg tggacggagg
     2401 gcagacgctc accgctctcg gcggctgatg acgacgaagg tcgccgtcgt cacgggggcc
     2461 tcgtcgggga tcggggaggc cacggcccgg gcgctggcaa gcgaggggct ccggctggtg
     2521 ctggcggcgc ggcgcgagga ccggctgcgg gcgctggctg aggagctcgg aggcgaggtt
     2581 ctgcccgtgc ggtgcgacgt cacgctgaag aaggacctgc gggcgctcgt ggccgcaacc
     2641 gaggagcggt tcggccgctg cgacgtcctg atcaacggcg cgggcgtgcc cgggagggga
     2701 cggttcgccg acgtcgacct cggccagctg gaggcggtga ccttcaccaa ctacctcggg
     2761 ctgctccggg ccacgaagct gttcctgccg atgctgctgg aagcacgcgg ccacgtggtg
     2821 aacgtcgcct cgctggccgg gctgttcgcc ctgccgggag cggaagccta cgccgcgtcg
     2881 aagcacgccg tggtcgcgct gtccgagtcg ctctcgctct cgctcggtga cgcgctcaag
     2941 gtgacggccg tctgcccggg cttcgtgaac acggaggggt tcccgcagcc ggagaagccg
     3001 cgccccgtaa cggtgcccct ggaggtggtg gccgagggga tcgtggacgt gctgcgtcgc
     3061 ggtcggacgg gcatggtggc catccccgcc tggccgggcc ccgtctcggc cgtccagcgc
     3121 ctgatcccac gcctctaccg gagagccctc cagcgaggga tcgggaggtt ccggcctagg
     3181 tgacgacctt gacgttgagg gccggcgagc ccatggcccc catcatgcgg atccacagga
     3241 gaaggtaggc ctccatcccg cgcgcggccg agaggtcccc gaggtccatg atgtgccgcc
     3301 agccgaagga ccgcaggatc tcaccgacgg cggccttcgc ctcctcgtcg tcgccgcaca
     3361 cgaagatcgt gtggtcgccg ccccccaccc cggcagggtc caccatgacc cgcgcgttca
     3421 cggtgttgag cgccttcacc accttggcgt gcggatgctg gcgctggatc cgctcggcga
     3481 gggagtcccc gctcgggacg aagagcttca tcgccccctc ctcgaactcg agggggttgg
     3541 agacgtcgac cagcaccttc cccccgacct ggcgatcgtc ggcggaggcc agcgcgtcga
     3601 gggagccctc cccgcgcgtg cagttgaaga gcatctcgcc gtgcgcggct gcctcggcga
     3661 acgtcccgac ggtggccccc ggcgtccgct ccatccactc ggagaacgac tcgctctccc
     3721 gctcccccgc cgggcgcgca gtgagcgcct cgacgtcccg cgtgcccagc atcacctcgt
     3781 gcccgagctc ggccagcttc cctccgagcg ctcgccccac cgatcccgtc cccagcacac
     3841 cgatccgcat ccagtcctcc ttcagtacag gaacatcggc ttgcccccga cgtgacggac
     3901 ctttggccgg tactgctcca cgtcgtacag ctcgtccacg tccacgcgct tgacgccctc
     3961 acgggtcacg gagatcggga cgttcgtgta ggtgcccccc cgcagggcca ccatccggcc
     4021 ggacgacccc tcgagcgcga ggtcggcggc catcaccgcg tagttcgtgg ccaccatgag
     4081 gtcgagcgag tcggggctgc ccgaccggac gaggtacccg agctgctgct ccacgatccc
     4141 ctcgccggtg atccgcttga tcagctgcgc cacctgggag ccgatcccac cgagcttccg
     4201 atggccgaag gcgtcttcct cgcccgagag catgaggtcg ccgccctcga tcacggctcc
     4261 ctccgagatc gtgagcatcg cgtagttgct gggattcgtc cgcttgtcct cgacgaggag
     4321 gcccgccagc cgctcgacgt cgacggggac ctcggagatc gcggctcggt ccaccccggc
     4381 caggtacgcg gtgatcagcg aggtctcgcc gctgtagcgg ccgaacagct cgacgaccgc
     4441 gatgcgctcg tgggaccccg tgctggtgcg aaggttgtgg atgaactgga cgcctcgggt
     4501 cacggcggtg gagaacccga tgcagtagtc ggtcccgtgg acgtcgttgt ccatcgtctt
     4561 cgggacggcg atgacgggga ccccctcctc gtgcatccgg agcgcgtacg agagcgtgtc
     4621 gtcgcccccg atcgggatga gcacatcgat agtctgcgac tcgaggaccc gaagcacgtg
     4681 cggggtgaag tcgaacggct cctccccgct cggctccagg aacgagggca cgtccttggc
     4741 tcgcaccctg cccgggttcg tccgggaggt gtggagcacg gtgccgccgg tgcggtcgat
     4801 cgtgcggacg gccgctcggt cgaggggccg gaggttggac gggacggtgt ttggctcgtc
     4861 gaggttcgtg gagaggaggc cgtcccagcc gcggcggatg ccgaccacct cgtggccctg
     4921 gtcggcgacg cgctcgacca cggccttgat gcaggcgttg agcccgggga cgtcgccccc
     4981 gcccgtcagg atcccgatgc gcatccgtcg ctcctccgcg ccgacccgcc catcgtagcg
     5041 agggtcaacg cgacagcggc cacaccatga ggaggccgtg gcgcttcagg gctagcgacc
     5101 cgtagaggtc gagggcggcg ccctggtcct cgtgcgtgtg caggacgatc gagtcgcagc
     5161 cggcaccctc ggcccggcgg cgcgcctcca ccatcagcgc ccggccgatg ccgcggcgac
     5221 ggtggctctc gtccacagcg agcccgtcga gccacgcccc cggggtggcg tccatcgggt
     5281 tgaagcgcgt gaacagcagc gtgcaggccc cgaccacgcg cccctccgac tcggccacga
     5341 ggaccgcgcc gtcctgcgag aggaagctct caaggccggg gcgggcgcgg tggtcgccgg
     5401 gcgggatggt cccccccacg aaggccatca gccgggcgac gtcgtcggcg tcctcgggca
     5461 aggcgtcacg gatcgagaag ccgctcaccc gcgaagccta accgagctac cggccggggg
     5521 cgtcgggggc cgtgccgcct ccgtgcggcc cggccggggg cggcctgcct ggccccgggc
     5581 ctccgggctt cccggggagc ggcccggcgc ccggcccccg gcccggc
//
LOCUS       DASUTW010000218         3118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1232035, whole genome shotgun sequence.
ACCESSION   DASUTW010000218 DASUTW010000000
VERSION     DASUTW010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232035"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VF058_07445"
     CDS             <1..406
                     /locus_tag="VF058_07445"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208175.1"
                     /translation="QLLQLIAKNELIALVSDRDLRGRGVDVEMFGATRKLPAGPALLS
                     LTTGSPLLPSAVYDTPEGWLCYVTEPITVERSDVLRDDVTALTRKLAARFETFIAEAP
                     TQWHMWQPAWPEDEVDAPHGSGLEPAPAEGPG"
     gene            403..1497
                     /locus_tag="VF058_07450"
     CDS             403..1497
                     /locus_tag="VF058_07450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6208176.1"
                     /translation="MRIALACPYAWDAPGGVQVHVAQLAERLRGRGHRVLVVAPSRGR
                     IDDPHLVSIGGTIPVPVNGSVAPIAPRPAAARRTRQALDDFGPDVVHAHEPFVPSASM
                     FAVRWGGAPVVATFHAYADRSPLLAAAAPILRPVWDRLAVRMAVSEAARRFVSHRFRG
                     EIRVVPNGVDVGLFRDARPADLPAGRRVLFVNRLDPRKGFAVMVGAFELLLGAHPDVL
                     LVVAGDGKERAAVSDLPHAARDRIVMLGNVPHDLLPPYHAACEVFAAPAVGRESFGIV
                     LVEAMAAGLPVVASDIPGYREVVRRGDDGLLVPPRDAAALAAALRRVLDDRDLAERLR
                     EAGRARSARYSWETVTRQVETAYEEAVGGA"
     gene            1487..2413
                     /locus_tag="VF058_07455"
     CDS             1487..2413
                     /locus_tag="VF058_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX6208177.1"
                     /translation="MELESFARTLATAIREAVLPMFGHPDARRRAGTAAGGDPTFAID
                     EVAERTAQALVEQRGDVAYFTEDAGLRTLGRPEGLLLLDPVDGSRPAVAGYETCGVSV
                     AVAPFGEGVTMADVRYGCVVEIATGAVFEARRGGGARAEGAEIRPSGATEPRGMLWAG
                     GFRGQPVVPTAVALEDLFDAPDAEGAFFDQGSAAYSLTRLATGQLDAYVDVGQALVEE
                     IPGTEGRFREVGGGHLLNTTTYDTAAGYLLLEELGLPVTDARGGPVGEVPLFAEDGAA
                     SLVSTVAACTPELHEALLEAVSRGIARLRSGA"
     gene            complement(2417..2857)
                     /locus_tag="VF058_07460"
     CDS             complement(2417..2857)
                     /locus_tag="VF058_07460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX6208178.1"
                     /translation="MNLTLTKRGDYAVRAAIHLARRWGDGLAKLREISADMELPRSYT
                     PQVLGILVRAGVAEAKAGRDGGYQLVRDPASISLLEVVEAADGPLRTTECTLRGGSCR
                     WDDACAVHPSWVAASEAFRASLASTSLADVAGVEPTLERRRAEG"
BASE COUNT          371 a         1094 c         1207 g          446 t
ORIGIN      
        1 gcagctgctg cagctcatcg cgaagaacga gctgatcgcg ctcgtctccg atcgggacct
       61 ccgggggcgg ggggtcgacg tcgagatgtt cggggccacg cggaagctgc ccgcgggtcc
      121 cgcgctgctc tcgctgacga ccgggtcgcc gctgctcccc agtgccgtgt acgacacgcc
      181 cgagggctgg ctctgctacg tcacggagcc catcacggtc gagcggtcgg acgtcctccg
      241 ggacgacgtg accgccctca cgaggaagct cgccgcccgc ttcgagacgt tcatcgccga
      301 ggctccgacg cagtggcaca tgtggcagcc ggcctggcca gaggacgagg tggacgcgcc
      361 gcacggctcc gggctcgagc cggcccccgc cgaagggccg ggatgaggat cgccctggcc
      421 tgcccgtatg cgtgggacgc accgggcggg gtgcaggtcc acgtggccca gctggccgag
      481 cggctccgcg gccgcgggca ccgcgtcctc gtcgtggcac cgtcccgcgg gcggatcgac
      541 gatccgcacc tcgtctcgat cggcgggacg atccccgtcc ccgtgaacgg gtcggtcgct
      601 cccatcgcgc cccggccggc cgcggccagg aggacgaggc aggcgctcga cgacttcggc
      661 ccggacgtcg tccacgcgca cgagccgttc gtgcccagcg cctcgatgtt cgccgtccgg
      721 tggggcggcg ctcccgtggt ggccacgttc cacgcctacg cggatcggtc gccgctgctc
      781 gccgcggcgg ctccgatcct ccgacccgtc tgggaccggc tggctgtgcg gatggcggtc
      841 tcggaggcgg cccgccgctt cgtctcgcac cggttccggg gtgagatccg cgtcgtcccc
      901 aacggcgtgg acgtgggcct gttcagggac gcgaggcccg ccgacctccc cgcgggtcgg
      961 cgggtgctgt tcgtcaaccg gctcgacccg cggaaggggt tcgccgtgat ggtcggggcg
     1021 ttcgagcttc tgctgggggc tcaccccgac gtgctcctgg tggtggcggg ggacgggaag
     1081 gagcgggccg ctgtctcgga ccttccccac gccgcccgcg accggatcgt gatgctcggg
     1141 aacgtgccac acgacctgct gccgccctac cacgccgcgt gcgaggtctt cgcggcccct
     1201 gccgtgggga gggagagctt cgggatcgtg ctggtggagg cgatggcggc gggcctgccg
     1261 gtcgtggcga gcgacatccc cggctaccgc gaggtcgtcc gcagggggga cgacgggctc
     1321 ctcgtcccgc cccgagacgc ggccgccctg gccgccgcct tgcggcgggt gctggacgac
     1381 cgggacctcg cggagcggct ccgcgaggcc ggacgggctc gctcggcccg ctactcctgg
     1441 gagacggtca cgcgccaggt ggagactgcc tacgaggagg ccgtcggtgg agcttgagtc
     1501 gttcgcccgc acgctggcca cggccatccg ggaggccgtc ctccccatgt tcgggcaccc
     1561 cgacgcccgg cgccgggccg ggacggcggc cgggggcgac ccgacgttcg cgatcgacga
     1621 ggtcgccgag cggacggccc aggcgctcgt cgaacaacga ggagacgtcg cgtacttcac
     1681 cgaggacgcg gggctccgca ccctgggccg gcccgagggc ctcctgctgc tcgaccccgt
     1741 cgacggcagc cgtcccgcgg tggccggcta cgagacctgt ggggtctcgg tggcggtggc
     1801 cccgttcggc gagggcgtga cgatggccga cgtgcgctac ggctgcgtgg tcgagatcgc
     1861 caccggggcg gtgttcgagg ctcgccgggg gggaggcgcg agggccgagg gagccgagat
     1921 caggccgtcg ggcgcgaccg agcccagggg gatgctctgg gcgggcgggt tccgcggcca
     1981 gcccgtcgtc cccacggccg tcgccctcga ggacctgttc gatgcgccgg acgccgaggg
     2041 ggccttcttc gaccagggga gcgcggcgta ctcgctgacc cggctcgcca ccggtcagct
     2101 cgatgcctac gtggacgtgg gccaggcgct cgtggaggag atcccgggga cggaggggag
     2161 gttccgggag gtcggcgggg ggcacctcct caacacgacg acctacgaca cggcggccgg
     2221 ctacctcctg ctggaggagc tcggcctccc ggtcaccgat gctcgcgggg ggccggtggg
     2281 tgaggttccc ctgttcgcgg aggacggcgc cgcgtccctc gtgtctacgg tcgccgcctg
     2341 cacccccgag ctgcacgagg ccctgctgga ggcggtgtcg cgggggatcg ctcggcttcg
     2401 ctccggggcc tgaccgtcag ccttcggcgc ggcgccgctc cagggttggc tcgacgcccg
     2461 ccacgtcggc caggctcgtg gaggccagcg acgcgcggaa ggcctccgac gcggcgaccc
     2521 aggaggggtg caccgcgcac gcgtcgtccc agcggcacga gcccccacgc agggtgcact
     2581 cggtcgtgcg gagggggccg tcggcggcct ccaccacctc gaggaggctg atggaggcgg
     2641 gatcgcggac gagctggtac ccgccgtcgc gtcccgcctt cgcctccgcg acccccgccc
     2701 gcaccaggat ccccaggacc tgcggggtgt agctgcgggg cagctccatg tcggcggaga
     2761 tctcgcggag cttggccagg ccgtcgcccc agcgtcgggc gaggtggatc gcggcccgga
     2821 ccgcgtagtc gccccgcttc gtcagcgtga gattcatcca gtgatccttc tgtgaccttg
     2881 cgactgaacg tttagtacgg tactctacat gctgaatgga gtcaaggaac ggccctagga
     2941 tcggccttga ggagggatga cgtatgcggt tcctgaagat gtgggtcgcc gcgaccgtga
     3001 gcgtgtggct cctggtcgtc ggcgcggtga tggcgggcgt gggcggctcg tccgaggacg
     3061 tcgccgtcgc cgcgcaggac tcggcgttcc ggatcgaggg ggaggggacc ggtgagat
//
LOCUS       DASUTW010000219         3143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1881149, whole genome shotgun sequence.
ACCESSION   DASUTW010000219 DASUTW010000000
VERSION     DASUTW010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881149"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1926)
                     /gene="nuoL"
                     /locus_tag="VF058_07465"
     CDS             complement(<1..1926)
                     /gene="nuoL"
                     /locus_tag="VF058_07465"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX6208179.1"
                     /translation="MIEQAWLIPVIPLVAFFLIAFFGKRSPGQGAGLGILAVGVSWVL
                     SLIVAFEQIGGEEAVERSWPWLEIGSFRFEIGTYVDGLAAMMLIVVTTVSLLVHVYSL
                     GYMRGDRRFTWFYAVLSLFTGSMLNLVLANNLWQLLIGWELVGICSYLLIGHWWEEKE
                     NSSAAIKAFITTKTGDVPFQFGIFALVIGVGSANIQEVTRAVEGGELSTTLTLAAALL
                     LFGGAIGKSAQFPLHVWLPDAMAGPTPVSALIHAATMVVAGIYLVGRMFLVFEAAAPE
                     ALTVVAVIAAITMLIAAVLAAVQDDIKRVLAYSTVSQLAYMMAGLGVGEAGYTAGFFH
                     LFTHAFFKALLFLAAGSIIHAVHTNNMSEMGGLRRHMPWTFWTFVIGAVALAGIPPLA
                     GFWSKDEIITAAYAEGQIGLWIVALITAALTAFYMTRAVLLTFFGRPRHSEEVHPHES
                     PPVMVGPLAVLAGLSLVIGFVNAPFVGWFSDWVFVHEPHHAEFSFFNAGLSLLIALGA
                     IALGVALYGRYREREPLRRLGPVYAFAERKYFLDDIYMGGIVEPVKGPIARGVYWTNQ
                     HILDGLVNAAGWIARKLGRGTEAVDRNVVDGIVNRVGVGTGWTGGLLRYVQSGNVQRY
                     AIVLFAGVAILAVIFTRI"
     gene            complement(1931..2239)
                     /gene="nuoK"
                     /locus_tag="VF058_07470"
     CDS             complement(1931..2239)
                     /gene="nuoK"
                     /locus_tag="VF058_07470"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015158031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX6208180.1"
                     /translation="MIRLPLVLFFSAGLFSVGVYGVLARRNAVLVLMSIELMLNAVNV
                     NLVAFSQLLQDTMMTGQIFALFVITVAAAEVGIGLAIVILIFRNRETINVDEITLLRW
                     "
     gene            complement(2236..2730)
                     /locus_tag="VF058_07475"
     CDS             complement(2236..2730)
                     /locus_tag="VF058_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX6208181.1"
                     /translation="MTWQEWAFVVLGLVGLGAALRLVTSKNVVHAALYLVVSLLMVGA
                     IYLLLGAEFIGWVQILIYVGAIVVLLLFGLMLTRAPIGREALDNQQRGLAAIVSSGIL
                     FGLTFLVQDAFGEQEIELQPTRIADAGERLFTDFVLPFEVVSFLLLAALIGAVALARK
                     EDAE"
     gene            complement(2727..>3143)
                     /locus_tag="VF058_07480"
     CDS             complement(2727..>3143)
                     /locus_tag="VF058_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208182.1"
                     /translation="LRDWTYKVKPPPELEWGAEPEPPEGEEDVAPTSVTPTAQPGEAA
                     PEETAPTVAPERAAEAPPERAAEAPPEQAAPADAPQAPAAPEQAAPAARQEVEPDQAT
                     YDRVLQEQLDAGVNPRVAEGRAKAAAIRAARQGGVG"
BASE COUNT          551 a         1127 c         1058 g          407 t
ORIGIN      
        1 gatcctcgtg aagatcaccg ccaggatggc gaccccggcg aacagcacga tcgcgtaccg
       61 ctgcacgttc cccgactgga cgtagcggag caggccaccg gtccagccgg tcccgacgcc
      121 cacgcggttc acgatgccgt ccacgacgtt ccggtccacg gcctcggtcc cccgccccag
      181 cttccgggcg atccagcccg ccgcgttcac cagcccgtcg aggatgtgct gattggtcca
      241 gtagaccccg cgggcgatgg ggcccttcac cggctccacg atcccgccca tgtagatgtc
      301 gtccaggaag tacttccgct ccgcgaacgc atacacggga cccagccgcc ggagcggctc
      361 gcgctcgcgg taccgcccgt agagcgcgac gccgagcgcg atcgctccga gcgcgatcaa
      421 gagcgacagc ccggcgttga agaacgagaa ctcggcgtgg tggggctcgt ggacgaacac
      481 ccagtccgag aaccacccca cgaacggggc gttcacgaac ccgatcacga gcgagagacc
      541 cgcgagcacc gcgagcggtc ccaccatgac gggcggggac tcgtgggggt ggacctcctc
      601 gctgtggcgc ggccggccga agaaggtgag caggaccgcc cgggtcatgt agaacgcggt
      661 cagcgcggcc gtgatcaggg ccacgatcca cagcccgatc tgaccctccg cgtaggccgc
      721 ggtgatgatc tcgtccttgg accagaagcc ggccagcggc gggatcccgg ccagcgccac
      781 cgccccgatg acgaacgtcc agaacgtcca cggcatgtgc cgccgcaggc ctcccatctc
      841 gctcatgttg ttcgtgtgga cggcatggat gatcgagccg gccgccagga acagcagggc
      901 cttgaagaac gcgtgggtga agaggtggaa gaacccggcc gtgtagccgg cctcccccac
      961 gcccagcccc gccatcatgt aggcgagctg ggacaccgtg gagtaggcca gcacccgctt
     1021 gatgtcgtcc tggaccgccg ccagcaccgc ggcgatcagc atcgtgatcg ccgcgatcac
     1081 cgcgaccacg gtgagggcct cgggggccgc ggcctcgaag acgaggaaca tgcgccccac
     1141 gaggtagatc cccgccacca ccatcgtcgc ggcgtggatc agggccgaca cgggcgtggg
     1201 gcccgccatc gcgtccggca gccacacgtg cagcgggaac tgggccgact tcccgatcgc
     1261 ccctccgaac agcagcagcg ccgccgcgag ggtcagcgtc gtggagagct ctccgccctc
     1321 cacggcgcgc gtcacctcct ggatgttcgc cgagcccacg ccgatcacga gggcgaagat
     1381 cccgaactgg aacggcacgt cacccgtctt cgtggtgatg aaggccttga tcgcggcgct
     1441 cgagttctcc ttctcctccc accagtggcc gatgaggagg taggagcaga tgccgacgag
     1501 ctcccagccg atcagcagct gccagaggtt gttcgccagg acgaggttga gcatcgatcc
     1561 ggtgaacagg gacagcaccg cgtagaacca ggtgaagcgt cggtcgcccc gcatgtagcc
     1621 cagcgagtac acgtgcacga gcagcgagac cgtcgtgacg acgatcagca tcatcgcggc
     1681 caggccgtcc acgtaggtcc cgatctcgaa ccggaacgac ccgatctcga gccagggcca
     1741 ggagcgctcg acggcctcct cacccccgat ctgctcgaag gcgacgatca gcgagagcac
     1801 ccaagagacc cccaccgcca ggatccccag ccccgcgccc tgtccggggc ttcgcttccc
     1861 gaagaaggcg atgaggaaga aagcgaccag agggatgacc gggatcagcc acgcctgctc
     1921 gatcatcgac tcaccatcgc aggagggtga tctcgtccac gttgatcgtc tcgcggttgc
     1981 ggaagatcag gatcacgatc gccaggccga tgcccacctc ggcggccgcc accgtgatca
     2041 cgaagagcgc gaagatctgg ccggtcatca tcgtgtcctg gaggagctgc gagaacgcca
     2101 ccagattcac gttgacggcg ttcagcatca gctcgatcga catcagtacg aggacggcgt
     2161 tgcgccgggc aagcacgccg tagaccccca ccgagaagag gccggccgag aagaagagga
     2221 cgagcgggag ccggatcact ccgcgtcctc cttccgggcc agggcgaccg ccccgatcag
     2281 cgcggccagc aggaggaagg agacgacctc gaacgggagg acgaagtccg tgaagaggcg
     2341 ctcccccgcg tcggcgatcc tcgtgggctg gagctcgatc tcctgctcgc cgaaggcgtc
     2401 ctgcacgagg aaggtgaggc cgaagaggat ccccgacgac acgatcgccg ccagccctcg
     2461 ctgctggttg tcgagcgcct cgcgcccgat cggcgcacgg gtgagcatca gcccgaagag
     2521 caggagcacc acgatcgcgc ccacgtagat caggatctgg acccacccga tgaactccgc
     2581 tcccaggagg aggtagatcg cccccaccat gaggagggac acgacgaggt agagggccgc
     2641 gtgcacgacg ttcttcgagg tgacgagccg aagggcggcg cccaggccca caaggcccag
     2701 gacgacgaag gcccactcct gccaggtcat cccacgcccc cctgtctggc ggcgcggatc
     2761 gccgccgcct tggcccgccc ctccgcgacc cgggggttga cgccggcgtc gagctgctcc
     2821 tggaggaccc ggtcgtacgt ggcctggtcg ggctcgacct cctgacgggc cgcgggggcg
     2881 gcctgctcgg gcgccgccgg cgcctgcgga gcgtcggcgg gggcggcctg ctcgggcggg
     2941 gcctcggccg cccgctcggg cggggcctcg gccgcccgct cgggcgccac cgtcggcgcc
     3001 gtctcctcgg gcgcggcctc gccgggctgc gcggtcgggg tcaccgaggt cggggccacg
     3061 tcctcctcgc cctccggggg ctcgggctcg gccccccact cgagctcggg cggcggcttc
     3121 accttgtagg tccagtcccg cag
//
LOCUS       DASUTW010000220        10107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_194892, whole genome shotgun sequence.
ACCESSION   DASUTW010000220 DASUTW010000000
VERSION     DASUTW010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10107
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194892"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..794
                     /locus_tag="VF058_07485"
     CDS             96..794
                     /locus_tag="VF058_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6208183.1"
                     /translation="MGAATVLVVDDERKIRDLVRSYLEREGYSVLQADSGQGALEAAA
                     RGRPDLVVLDLMLPDLAGEEVARSLHEIAPLPIIMLTAKAGEDDRIAGLRLGADDYLV
                     KPFSPRELVARVEAVLRRARGGRTDSSLSLDGGRLTVDPEAREVVVDGESVALTRSEF
                     DLLAALASRPGRVFSRYELVTRVQGYDFEGYERTIDVHVKNLRRKLGDDPRDPRYVVT
                     VTGVGYKMAARADA"
     gene            787..2199
                     /locus_tag="VF058_07490"
     CDS             787..2199
                     /locus_tag="VF058_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007498310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6208184.1"
                     /translation="MRRSFAARLAAAFAGVGILAAAITALLVNMAFGGRFDAYLTDQR
                     EARERAIVAGLADSYRRMESWDTGDLDTLAPLAIMDGGTLALESPAGDVIWTARATGS
                     MAAMHRSMMGTGALGPERRLPVIVRGDRMGVAVVRLPQAGVLPSDLAFRASINRLLLY
                     GGAAAAAVALALGIILARRATAPARALTRAARSLAEGDRSRRIASGARDEFGEMAAAF
                     DQMAEALEEQDRLRRSFAADVAHELRTPLSILRSEVEALQDGVRSAGPETFGSLHEEV
                     LRLSRLVADLETMAAADAAGFSLKRERVDLGAVARSVVSEMAGAFESAGVGIRVEAGD
                     VEVDADPVRVRQMLGNLLSNAMKFTPVGGEVRVEVSAQDGCAVLRVADTGPGIPAEEI
                     PRVFDRFFRGAGVRAGGSGVGLAVVRELARAHGGEVEVASRPGEGTAFTIRLPQTAPG
                     LRHVFTTPSHPPTTVPVKGA"
     gene            2206..2487
                     /locus_tag="VF058_07495"
     CDS             2206..2487
                     /locus_tag="VF058_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208185.1"
                     /translation="MQTRWAIVVGVALLVVTLVVGLALAAPGGRSGSGWWDEIHGSAS
                     MRRMHSQMPGDLRAQCDAMHAQMHEWMDEQMGGGGMMGGGGMMGGGTGP"
     gene            2651..3175
                     /locus_tag="VF058_07500"
     CDS             2651..3175
                     /locus_tag="VF058_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208186.1"
                     /translation="MEEVAVESPEATPTRVGVVSPRMVPRSLRVGAVAAAGMAIFYVT
                     VVWGLSGSLDHLLEQLRLDWYLLLPIVAGFGVQVGLVAELRRRHSARRAAMAAGATGA
                     GSSTVGMIACCVHHLADLAPLVGAGAAASFLVDYRVPFMVAGIAINAAGIAVAARALR
                     HLGGAREEDACDAH"
     gene            3160..3594
                     /locus_tag="VF058_07505"
     CDS             3160..3594
                     /locus_tag="VF058_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208187.1"
                     /translation="MRRALIVVVPLVVAAGLPLLVVTLPDSGAPGSERAPRVLSPRTV
                     SAGPIEVTVAPVSVEGREAVFEVAFDTHSVELDLDVAGASSLEVGGIAWAPGTWEGDG
                     PGGHHRAGRLSFAPGGPVSGDAHLTIRELPAPVHLQWMLGGG"
     gene            3624..3938
                     /locus_tag="VF058_07510"
     CDS             3624..3938
                     /locus_tag="VF058_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208188.1"
                     /translation="MGRWIGAASVLLVAGGIAAAGLRGGYLWALLLVGLLLLVPLLAG
                     ASLRSDDRGSKEEEGVGSEGAGVRGGIERRSRAGWWVGGFRERLQTGGNDATRPPGGG
                     SR"
     gene            3947..4186
                     /locus_tag="VF058_07515"
     CDS             3947..4186
                     /locus_tag="VF058_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEX6208189.1"
                     /translation="MVTAPGCHLCADAREALATLARQFPLEVREVGASSPEGLTLVAK
                     HRPALQPLVLVDEVFFSAGRLPRRKLRRRLERRVA"
     gene            4183..5130
                     /locus_tag="VF058_07520"
     CDS             4183..5130
                     /locus_tag="VF058_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HEX6208190.1"
                     /translation="MSLLVGGSILAAFFAGSVALFSPCCIVFLLPAYLASAVKERRWA
                     LFPLTLVFAAGLAVVILPLTLGVGMLASTLTRFHTGFYLAGGVLMLALGALAFTGRSW
                     SIPGLRRSPALDRSDVPGVFTLGVFSGIASSCCAPVLAGVVTLSALAGSLWGSAALGL
                     AYVFGMVFPLFLVALVWDRLRLGERRFLAGREIAIRLGRRVARTTTVNLVVAGAFTLM
                     GAGVIVLAVTGAEATAPGFQLAIGRWLSRAFGWITDAVKGVPEPLLGLGLVALAAVIA
                     WMGVRGARTGSTSDETKESCHGTEEQREEEAAFAAGPEA"
     gene            5072..5680
                     /locus_tag="VF058_07525"
     CDS             5072..5680
                     /locus_tag="VF058_07525"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HEX6208191.1"
                     /translation="MGRKSNAKKKRRSQPARKPRRRIGSGTAALVGAILGIAGLLWLG
                     SRGGTEALATGEPAPEFTLPSTGGGAVSIEDLRGGNVLLYFNEGAGCGSCFTQLVELE
                     RHSGHLGELDITLVPISPNPLETTVAEAARYGVRTPFLIDDTLEVASAYDTLGTGHHG
                     GLPGHSFILLDDQGRYRWRGDYPGMWVSPEELLSRTRASLAT"
     gene            5708..6562
                     /locus_tag="VF058_07530"
     CDS             5708..6562
                     /locus_tag="VF058_07530"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX6208192.1"
                     /translation="MLGTISWTPLKEIHLGPVVLYPHGLLIAVGFVAGALLLRRYTRR
                     RGIPDAVVWRILTWVAVGSLVGMRLAWVAGNWRMLSSPLEVFALWHGGMSLLGGIIGG
                     TLAALPALRREDLPVLPMFDLAAPGLALGIVIGRFSDLVIGDHLGKPTGLPWGFRYTG
                     GEVPGTAPAVGSVVHPVALYDMVSTGLLLLALAWLLQKPRAPGSAISLFVVWYGAFRL
                     ATDFLRTDPVRGFGMTGSQLTSLIAIVAVLAALLVRARREGARPRHGSLTAHSHDGPR
                     LSGWLGNL"
     gene            7219..>10107
                     /locus_tag="VF058_07535"
     CDS             7219..>10107
                     /locus_tag="VF058_07535"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6208193.1"
                     /translation="MGRAPEMHALLSALDDGASGHGRIVLIGGEPGIGKTRLVDEFAA
                     VARARGFLLVSGRSWEAGGAPAYWPWTQAIRGLLRELSTEALKAALAGGGPHLAQMLP
                     ELRERLPDLPDPPRGSPEVVRFRLFDALAGLLRRVAEDRVMLVVLEDIHAADASSLLL
                     LRFLAEEIQASRLLLLATYRDIELTKDHPLAAVLPELTRASGTARISLSGLGQRDVSR
                     FIEALTEEVPPADLVAEVHRGTEGNPLFLIEVVGLLSSEGRLGDALERFPIPEGVRTV
                     IGRRLDRLSGECRRILRLGSVMGRDFRIHVLERVAGRPAHRILDALGEAVGTRTVAPV
                     EDDPGRFRFTHVLLREVLYDSLPPADRVRAHGEVGEALETFYSDEMEPHVAEIAHHFF
                     EASPARGFAKAVDYATAAGRRATALLAYEEAVRLFRMGLRALQGSRDEESRCEILLLL
                     GDAEARSGDTPVAKESFLRAAEIATRLDQAEPLARAALGYAGRFPWVRAGIDRHVIPL
                     LKQAIDALGPTDSTLRVRLLSRLAGALRDQPWMEPREELAREAVSMARRIGDPETLAY
                     ALLGQWAAVFMGPDGLGRHAALGDELDHLSGLIGDRELEVSICAFRFTTGMARGEVAE
                     ARVQHETAARLTAELRQPPQRWLVGLMATMLALQDGRFDDAERLIDETFEAGRRAQEW
                     DAGAARLFSLFMLRREQGRLAELEEEIRRAPGHYSGYRSLRCMLLSSLCDLGRLDEAR
                     ALFEQLAVDDFARFPKDNEWLFAFTLLAEATHVLEDRDRAQSLYEQLRPYAERIAWLA
                     SDASAGPVGRPLGLLAALLGRRDEASGYFEDAIERAERMESRPWAAHARYAYAEMLAA
                     GERPEGSDRAVDLLAATLETCEDTGMIMLGRKAKGLMARLGVRPRTGAASGGLERGAV
                     LTPRELEVAGLVAEALSNRQIAERLYLSERTVESHVQSILAKLGMTSRT"
BASE COUNT         1306 a         3390 c         3732 g         1679 t
ORIGIN      
        1 tccgagacgc gctgaacggg ggcgacgccg aggcgaagac ggccgagctg ctggaggcgg
       61 tccgccggta cgtccggagc gcctgagggg ttcccatggg tgccgcaacg gtgctggtcg
      121 tcgacgacga gcggaagatc cgcgacctcg tccgctccta cctcgagcgg gaggggtact
      181 cggtcctgca ggccgactcg ggccagggag ccctggaggc cgccgcgcgg gggagacccg
      241 atctcgtcgt cctggacctg atgctgcccg acctggctgg ggaggaggtg gctcgttcgc
      301 ttcatgagat cgctcccctt ccgatcatca tgctcaccgc caaggcgggc gaggacgacc
      361 gcatcgccgg gctgcggctc ggggccgacg actacctggt caaacccttc agcccgcgag
      421 agctggtggc gagggtcgag gcggtgctcc ggcgggcccg cgggggccgg actgactcgt
      481 cgctctccct ggacgggggg aggctgacgg tcgatccgga ggcgcgcgag gtggtcgtgg
      541 acggggaatc cgttgcgctg accaggagcg agttcgacct tctggcggcg ctggcctccc
      601 gcccgggccg ggtcttctcc cgctacgagc tcgtgacccg ggtgcagggc tacgacttcg
      661 agggatacga gcgcacgatc gacgttcacg tgaagaacct ccggcggaag ctcggggacg
      721 atccgaggga cccccgctac gtggtcacgg tgacgggcgt cggctacaag atggcggcgc
      781 gggccgatgc gtaggtcctt cgcggcccgc ctcgctgcgg ccttcgccgg ggtcggaatc
      841 ctcgccgccg ccatcaccgc gttgctcgtc aacatggcgt tcggggggcg ctttgatgcc
      901 tacctcaccg accagcgcga ggccagggaa cgggccatcg tcgcgggcct ggccgattcc
      961 taccgccgga tggagagctg ggacaccggt gacctcgaca cgttggcccc gctggcgatc
     1021 atggacggcg gcaccctggc gctggagagc ccggcgggcg acgtcatctg gactgcgaga
     1081 gccaccggct cgatggccgc gatgcatcgg tcgatgatgg ggaccggcgc gctgggtccg
     1141 gagcgacggc tgccggtgat cgtccgaggc gaccggatgg gcgtggcagt cgtgcggctt
     1201 ccgcaggcag gcgtgctccc gagcgacctg gcgttccggg catcgatcaa ccgcctcctc
     1261 ctctacggag gagctgcggc ggccgcggta gcgcttgcgc tcgggatcat ccttgcgcgg
     1321 cgggcgaccg cccccgcccg cgccttgacg cgggccgcac ggtctctcgc cgagggtgac
     1381 cggagccggc ggatcgcctc gggagcccgc gacgagttcg gcgagatggc tgccgccttt
     1441 gaccagatgg ccgaggccct ggaggaacag gaccgcctgc ggcggtcgtt cgccgccgac
     1501 gtcgcgcacg agctccgcac ccccctctcg atcctccgct cggaggtgga ggccctccag
     1561 gacggggtcc ggagcgcggg tcccgagacc ttcgggtccc tgcacgagga ggtgctgcgg
     1621 ctgtcccgct tggtggccga ccttgagacc atggccgccg ccgacgcggc ggggttctcc
     1681 ctgaagcgcg agcgggtgga cctcggggcg gtagcgcgat ccgtggtttc ggagatggcc
     1741 ggcgcgttcg agtcggcggg agtcggcatc cgcgtcgaag ccggggacgt ggaggtcgat
     1801 gcggatccgg tgcgggtgcg acagatgctg gggaaccttc tctcgaacgc catgaagttc
     1861 acgccggtgg gcggggaggt acgggtcgaa gtgtcggctc aggacggatg cgccgtgctg
     1921 cgcgtggccg acaccgggcc ggggatcccg gccgaagaga ttccgagggt cttcgatcgt
     1981 ttcttccgag gggcgggtgt tcgtgccgga ggctcggggg tcggactcgc ggtggtccgc
     2041 gagctggccc gtgcccacgg cggggaggtg gaagtggcct cccgccccgg ggagggcacg
     2101 gctttcacga tccgtcttcc gcaaaccgca cctggtcttc ggcacgtctt cacgactcct
     2161 tcacaccccc ccactacggt gcccgtgaag ggagcgtgag aagcgatgca gacgcgatgg
     2221 gcgatcgttg tcggagtggc cctcttggtg gtgacactcg tggttgggct cgcgctcgcc
     2281 gccccgggtg gacgctcggg atccggttgg tgggatgaga tccatggttc cgcgtcgatg
     2341 cggcggatgc actcgcagat gcccggcgat ctccgggccc agtgcgacgc catgcacgcc
     2401 cagatgcatg agtggatgga cgagcagatg ggcggcggcg gcatgatggg cggcggcggc
     2461 atgatgggcg gcggcacggg cccgtgacgc gccgacgctg atccgctgcc aggtcggctc
     2521 gacgatccgc ccgggacacc cctctccccg ggcggattgc gcttctcgtg acacctgggc
     2581 cgccctcctc acactgagcg cgcaccgagt tcacggcccc ttcacatcgg gggccgatgc
     2641 tcgtctcaag atggaagagg tcgctgtcga gtcgcccgag gcgaccccaa cgcgcgtcgg
     2701 ggtggtgtcc ccacgcatgg tccctcggag cctgcgagtg ggggccgttg cggccgccgg
     2761 gatggcgatc ttctatgtga cggtcgtctg gggactctcg ggctcgctcg accacctact
     2821 cgaacagctc cgcctcgact ggtacctcct actgccgatc gtcgcggggt tcggcgtgca
     2881 ggtggggctc gtggccgaac tgcggcgccg ccactccgct cgtcgagccg cgatggcggc
     2941 cggggccacc ggggccgggt cgtccaccgt gggcatgatt gcctgctgcg tccaccacct
     3001 ggccgacctg gcccccttgg tcggggccgg ggcggccgca tctttcctcg tcgactaccg
     3061 ggtccccttc atggtggcgg ggatcgcgat caacgcggcc gggatcgccg tggcggcacg
     3121 ggcgctccgg cacctcggcg gggcgcgaga ggaggatgca tgcgacgcgc actgatcgtt
     3181 gtcgtaccgc tcgtcgtggc cgccgggctc cccctgctcg tcgtgaccct gccggacagc
     3241 ggcgctcctg ggtcggaacg ggccccccgc gtcctctctc cccggaccgt ctccgccggc
     3301 ccgatcgagg tcacggtggc tcccgtgtcc gtcgaggggc gagaggcggt cttcgaggtc
     3361 gcgttcgaca ctcattccgt ggagctcgat ctggacgtgg ctggggcctc ctcccttgag
     3421 gtcgggggga tcgcatgggc cccgggaacg tgggagggag acggcccagg gggacaccac
     3481 cgggccgggc ggctctcctt cgctccaggc gggccggtct ccggggatgc ccatctgacg
     3541 atccgcgagc ttcccgctcc cgtgcacctc cagtggatgc tgggaggtgg ctgagcatgc
     3601 aggccgggac gagacaccgc ccgatggggc gatggatcgg cgcggcctct gttctcctgg
     3661 tggccggggg gatcgcggct gccggccttc gcgggggcta cctgtgggcg ctcctcctcg
     3721 tcgggctgct gctcctggtc ccgctgctcg cgggcgcatc gcttcggtcg gacgaccggg
     3781 gatcgaagga agaggaaggg gtcggctccg agggggccgg cgtccgaggt gggatcgagc
     3841 ggcggtctcg cgccggctgg tgggtgggcg gattccggga acgcctgcaa acgggcggca
     3901 acgacgcgac ccggccccca ggcggcggat cacggtgagg atcaccatgg tgacggcgcc
     3961 cggatgtcac ctgtgcgcgg acgcccgtga agcgctggcg acgctcgccc ggcagttccc
     4021 cctcgaggtg cgggaggtgg gcgcgtcgtc ccccgagggt ctgactctgg tggcgaagca
     4081 ccggccggct ctccagcccc tggtcctcgt ggacgaagtg ttcttcagtg ccgggcgcct
     4141 gccacgcagg aagctccggc ggcgcctcga acggagggtg gcgtgagtct cctcgtcggc
     4201 ggctcgatcc tcgctgcttt cttcgcgggc tcggtggcgc tcttcagccc ctgctgcatc
     4261 gtcttcctgc ttcccgccta cctggcgtcc gcggtcaagg aacgccggtg ggcgctcttc
     4321 ccgctcacgc tggtgttcgc ggcggggctc gcggtggtga tcttgccact cacgctgggc
     4381 gtgggcatgc tcgcgtcgac cctgacgagg ttccacaccg gcttctacct ggccgggggc
     4441 gtcctcatgc tggccctggg ggcgctggcc ttcacggggc gctcctggtc gatccccggt
     4501 ctgcgccgct cgccagcgct cgaccgctcg gacgtccccg gggtcttcac gctgggcgtc
     4561 ttctcgggca tcgcctcctc ctgctgcgcc ccggtcctgg cgggagtcgt caccctctcc
     4621 gccctggccg gatcgctgtg gggatcggcg gcgctggggc tcgcctacgt gttcgggatg
     4681 gtcttcccgc tgttcctcgt ggctctggtg tgggatcgcc tgcggctggg ggagcggcgg
     4741 ttcctcgcgg gccgggagat cgcgatccgg ctgggccgcc gggtcgcgcg gacgacaacg
     4801 gtgaacctgg tggtcgcagg cgcgttcacc ctcatgggag cgggggtcat cgtcctcgcg
     4861 gtgaccgggg cggaggcgac cgccccgggg ttccagctcg cgatcgggcg gtggttgtcg
     4921 agggctttcg gatggatcac cgatgccgtg aagggggtgc ccgagccact cctcggcctc
     4981 ggcctggtgg ctctcgcggc cgtgatcgcc tggatggggg tccgcggcgc tcgcacggga
     5041 tcgacatccg atgagacgaa ggagtcgtgc catgggacgg aagagcaacg cgaagaagaa
     5101 gcggcgttcg cagccggccc ggaagcctag gcgtcggatc ggctccggga ccgctgccct
     5161 ggtgggggcg atcctcggga tcgccgggct gctatggctc gggtccaggg gaggcaccga
     5221 ggcgctcgcg accggggagc ccgcacctga gttcaccctg ccgagcaccg gcggcggcgc
     5281 cgtctccatt gaggacctcc ggggagggaa cgtcctcctc tacttcaacg agggtgctgg
     5341 gtgcggatcg tgcttcacgc aactcgtcga gctcgagcgg cactcggggc atctggggga
     5401 gctggacatc acgctcgttc ccatctcgcc gaatcctctg gagacgacgg tcgcggaggc
     5461 ggcccgctac ggggtgcgca cacccttcct catcgacgac acgctggagg tggcgagcgc
     5521 ctacgacacg ctcgggacgg gccaccacgg cggccttccc ggccacagct tcatcctgct
     5581 cgacgaccag ggcaggtacc ggtggcgggg tgactacccg gggatgtggg tcagccccga
     5641 ggagctcctg tcgcggacgc gagcctcgct cgcgacctga cccgttggga cggatggagg
     5701 agggggcatg ctcgggacca tcagctggac gccgctgaag gagatccacc tcggaccggt
     5761 cgtgctgtac ccgcacggcc tgctcatcgc cgtcggattc gtcgccggcg cgctgctgct
     5821 ccgccggtac acgcggcgtc ggggaatccc cgacgcggtg gtgtggcgca tcctcacatg
     5881 ggtcgccgtg ggatccctgg tggggatgcg gctggcgtgg gtggcgggca actggcggat
     5941 gctctcctcc ccgctcgagg tcttcgccct ctggcacggc gggatgagcc tcctgggcgg
     6001 catcatcgga ggaacgctgg cggcgctgcc cgcgcttcgc cgggaggacc tccccgtcct
     6061 tcccatgttc gatctggcgg ccccggggct ggcgctgggg atcgtcatcg gtcgcttcag
     6121 cgacctggtg atcggggacc atctcgggaa gccgacgggc ctgccctggg ggttccgcta
     6181 caccggcggc gaggtcccgg ggaccgcgcc ggccgtcggg tcggtcgtgc acccggtggc
     6241 cctctacgac atggtctcca ccggtcttct cctactcgca ctggcctggc tcctccagaa
     6301 gccgcgggcc ccggggtcgg ccatctcgct cttcgtggtc tggtacgggg cgttccgtct
     6361 cgccacggac ttcctgcgga ccgatcccgt gcgcggcttc gggatgacgg ggagccagct
     6421 cacctccctt atcgcgatcg tggcggtgct cgctgccctg ctcgtacggg ctcggcgaga
     6481 gggcgcacgg cctcgccacg gttccctcac ggcgcattca cacgacggcc ctcgcctctc
     6541 tggttggctc ggcaacctct aatcttgcct gctgccgtcc ttcccccccg tctgggaggg
     6601 agacatgact agcgacgtga ggtgacccac cgaatacgag acccgagctc agaggcggtc
     6661 ttcggcgctc cgctacaggt caccggcacc agaattggcc caccttgccg cggctttgat
     6721 caccaggcgg catcccgatt ctcgctccgg cgttggtcgg agtagtggtc acacttggtc
     6781 acaccctgct ggccgactcc gctaggcgcg cggccctagc tggagggtgg tcacacttct
     6841 ggtcggactc ggccgctttc tgggccgtcc ggcggggttc gaagcggtcc gctggatcgg
     6901 tgacaccccc gctgacctgc ggcgcgaacg ctgttgccac agacgtgcga ccccggctgt
     6961 gacctgcgga aacacgtgga gactatcgtg cttctcgcgg cggtcgtccc tccgccgccc
     7021 tcctactagg ctccgtgcct aacggctatc atctggtggg aagagagggc tcttcagggc
     7081 agcgatctgc gactcgtcgg acctccccaa gggcactcgg acagaggagg cgattgcgtg
     7141 tcgatccgac ctgtcccttc tgcgggcgac ccccaacgac acggccctca ggggctcgcc
     7201 cggggaggga gcctcttcgt gggccgcgcg cctgagatgc atgccctcct ttcggccctc
     7261 gatgacggcg cctcgggtca cgggcggatc gtcctgatcg gcggggagcc cgggatcggg
     7321 aagacgaggc tggtcgacga gttcgccgcc gtggcgagag cacggggctt cctgctggtg
     7381 tcggggcggt cctgggaagc gggcggcgca cccgcctact ggccatggac ccaggccatc
     7441 cgcgggctcc tgcgggagct cagcaccgag gccctcaagg cggcgctggc gggagggggg
     7501 ccccacctgg cgcagatgct gccggagctc cgagagcggt tgccagatct ccccgatcct
     7561 cccaggggat cacccgaggt cgttcggttc cggctcttcg acgctcttgc cgggctgctg
     7621 aggcgggtcg ctgaggatcg tgtgatgctc gtggttctcg aagacatcca tgccgctgac
     7681 gcttcgtcac tccttctgct gcgtttcctg gccgaggaga tccaggcgag ccggctgctc
     7741 ctcctggcga cctatcggga cattgagcta accaaggacc atccgctggc tgcggtgctg
     7801 cccgagctga cccgggcttc ggggaccgct cggatctccc tgtcgggact cggtcagcgc
     7861 gacgtgtctc ggttcattga ggccctcacg gaggaggtcc cgccggccga cctcgttgcg
     7921 gaggtccacc gcgggaccga ggggaaccct ctgttcttga tcgaggtcgt ggggttgttg
     7981 tcctcggagg gccggctggg agacgcgctg gagcggttcc ccatccctga aggggttcgc
     8041 accgtcatcg ggcggcggct ggaccgactc tcgggggagt gccgccggat cctgaggctc
     8101 ggctccgtca tggggcggga cttcagaatc cacgttctgg agcgggtcgc gggccgcccg
     8161 gcccatcgca tcctcgacgc cctcggtgaa gccgtgggta cgaggaccgt cgctccggtc
     8221 gaggacgacc caggacgctt ccggttcacc cacgtgctgc tccgggaggt gctctacgac
     8281 agcctgcctc ccgccgaccg cgtccgcgcc catggagagg tgggcgaagc gctggagacc
     8341 ttctactccg acgagatgga gccgcacgta gcggagatcg ctcaccactt cttcgaggcg
     8401 tctccggcaa ggggtttcgc caaggcggtg gactacgcca ccgccgcagg gaggcgtgcc
     8461 acggcgctct tggcgtacga ggaggccgtc cgcctcttcc ggatggggct gcgagctctg
     8521 caaggatcgc gagacgagga aagccggtgc gagatcctcc tccttctcgg ggacgcggag
     8581 gcccgatcgg gcgatacccc cgtggcgaag gagagctttc tccgagccgc cgagatcgcc
     8641 acccggctgg atcaggcaga accgttggct agagccgcgc tcggctacgc gggtcgtttc
     8701 ccctgggttc gggcgggtat cgaccggcac gtcatccccc tgctcaagca ggccatcgac
     8761 gccctaggac cgactgacag cacactgcga gtccgactcc tgagccgact ggcgggggcc
     8821 cttcgggacc agccgtggat ggagccgagg gaggagctgg ctcgtgaggc ggtctcgatg
     8881 gctcgccgca tcggcgatcc ggagacgctc gcctacgcac tgctggggca gtgggcagcc
     8941 gtcttcatgg gccccgacgg actgggtcgg cacgccgccc tgggagacga gctggaccac
     9001 ctgtctggcc tgataggaga ccgcgagctg gaggtgagca tctgcgcgtt ccggttcacg
     9061 acgggcatgg ccagggggga ggtcgcggag gccagagtcc agcacgagac ggcagcccgc
     9121 ctcaccgccg agctccgcca gcccccgcaa cgatggctcg ttgggctgat ggccacgatg
     9181 ctggcgcttc aggacggccg gttcgacgac gccgagcggc tgatcgacga gaccttcgag
     9241 gccgggcggc gggcccagga atgggacgct ggagcggcgc ggctcttctc gctgttcatg
     9301 ctccgtcggg agcaagggcg cctcgccgag cttgaggagg agatcaggcg ggcgccgggg
     9361 cattactccg ggtaccggtc gctgcgttgc atgctcctct cttcgctgtg cgatcttggc
     9421 cgcttggacg aggcccgggc tctcttcgaa cagctagcgg tcgacgactt cgcccggttc
     9481 cccaaggaca acgagtggct gttcgccttc acgctgctgg ccgaagcgac ccacgtcctt
     9541 gaggaccgcg accgcgcgca gagtctgtat gagcagctga ggccgtatgc ggagcggatc
     9601 gcctggctcg catcggacgc cagcgcaggc cccgtgggcc gcccgctggg ccttctggcc
     9661 gcgctcctcg gccgccgcga cgaggcgtcc ggatacttcg aggacgcgat cgagcgggcg
     9721 gagcgcatgg agtcccggcc gtgggccgct catgcgcggt acgcgtacgc ggagatgctc
     9781 gcggcgggtg aacgccccga gggatccgac cgagccgtcg acctgctggc ggcgactttg
     9841 gagacctgcg aggacaccgg catgatcatg ctgggccgga aggcgaaggg actcatggcg
     9901 aggctcggtg tgcgtccccg cactggggcg gcgagcgggg ggctcgagag gggagcggtc
     9961 ctcacacctc gggagctcga ggttgccggt ctggtggcgg aggccctatc gaaccgacag
    10021 atcgctgagc gcctctacct ttccgagcga accgtcgaga gccatgttca gagcatcctg
    10081 gccaagctgg ggatgacctc gcgcacc
//
LOCUS       DASUTW010000221         4606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_585984, whole genome shotgun sequence.
ACCESSION   DASUTW010000221 DASUTW010000000
VERSION     DASUTW010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585984"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VF058_07540"
     CDS             complement(<1..645)
                     /locus_tag="VF058_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208194.1"
                     /translation="MRRFLSPAGLALAAFCFFLPFVSVSCNPGALEDVIGEELPTTGP
                     TPFPGGFPGGLGQEVEVTAGTGWEIALGSEPELSETFGGLAELGGAGETTADEPWPGQ
                     AYAIAALALAVLGIVMALVLTGMPGALSGLIAGVGGAVMVFLIRTEISSWFEDELRAD
                     PQAGAILDLSFEIGWWLALAFFIVAAALAGWAMAAPRQPTEFAAPPPPATPPGGT"
     gene            complement(715..2061)
                     /locus_tag="VF058_07545"
     CDS             complement(715..2061)
                     /locus_tag="VF058_07545"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX6208195.1"
                     /translation="MADLGDLCATAVGAAESGEAVEAYAGQSRRTEVKARGGEVDSLA
                     RSESRGIGVRVMADGRLGYAWAADPDRDEVAGLVASAREAARHAAPDEANVLPEAEAA
                     EPLSGLFREAQAALGPERKVELALELDRASTSSHPDVRKVEEVIYADVVSRVAIASTA
                     GVRAERTRTDCWCMVSALAERDGETQSGFSFAVARELDELDWRGAAAEAAERGARLLG
                     ATKPSTERLPVVLDPWAASSFLGVLAGALSAEEVQKGRSLFAGKVGEAVGAEGLTLVD
                     DGRLPDGPGSAPFDDEGVPTGRTPLIEAGTLRGFLHNAQTAARGGTRSTGNAGRAGYR
                     SVPGVSPTNLVVEPGEDGPEAVLARAGTGAYVQSVTGVHSGANPVSGEFSVGATGLRI
                     EGGSLGEPLREMTIASTLQDVLGALVAIGSDLRFFPGGGAIGTPTMLVGEMTVAGA"
     gene            complement(2054..3439)
                     /locus_tag="VF058_07550"
     CDS             complement(2054..3439)
                     /locus_tag="VF058_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX6208196.1"
                     /translation="MLDPSLAREVLGAALAGGAAFAEIFAEDRRSTSGRLDDGKIEEF
                     SSGHDRGAGVRAVAGDTTAYAYSNRLDREALVGAAEAAAAAARGSDRASVLDFTRAEP
                     AVTHDPEEPTGGVPAARKVAWLQEADDAARAAGPEVRQVIALYGDSVQRLLIASSEGL
                     WVEEERHRLRLVVQVVAARGDALQTGFEGPAGLGGVEVLRAHPPAETGEKAARQAITM
                     LDGVPAPAGEMAVVVGPAGGGVLFHEACGHGLEADTVGKEASIYRGRLGETLGSTIVT
                     GVDDATVDHAWGSFSFDDEGSPAESTVLFEEGVLKSLLYDRFWARKDGAGPTGNARRQ
                     SYAHLPIPRMTNTSILPGKDDPGDIVADTPRGLYVKALGGGQVNPATGDFVFGASEAY
                     LIEDGRITTPARGANLIGNGIAILRAVDAVADDFDARPGTCGKDGQGVPVTTGSPTLR
                     IARMTVGGTGG"
     gene            3508..>4606
                     /locus_tag="VF058_07555"
     CDS             3508..>4606
                     /locus_tag="VF058_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208197.1"
                     /translation="MSRVSAARRAPTRGSRRRPRAAWPAALTWAGGVALVLLGVFLAH
                     PLLEGHRLPLGPDGPVYVWWSRFAEVEGLDAVRRPGGPALILALTSMLATDPLRVVAA
                     LEPILAAAIGLAGGAVAEAALGPDRVRFGTTSLLVGAFAGLLAAGWIANLMLAALFLT
                     ALAAFAVVDRSWRGVAFGSAALAAAGVAHSLFLAVALGIVAGAVILLAPDALRRIRAG
                     VRPLDTAAMRITAGAAAGGGGALLAVGLLAGGPTAPGETSQDQIFRRIGMRGTLSERY
                     RERLWGDARRMAIPAVAAILGAAWPGLLDGVDGERRRYAISVAASWVAVTVVGVAVLW
                     ATGLGPPGRLLTFAYVLPLAAAAAAASAMRRG"
BASE COUNT          531 a         1783 c         1700 g          592 t
ORIGIN      
        1 agtccctccc ggaggcgtcg cgggcggggg cggcgccgcg aactccgtcg gctgcctcgg
       61 ggcagccatg gcccaccccg cgagcgcggc cgcgacgatg aagaaggcca gggccagcca
      121 ccacccgatc tcgaagctca ggtccaggat cgcgcccgcc tgcgggtcgg cccggagctc
      181 gtcctcgaac cagctgctga tctcggtccg gatcaggaag accatcaccg cgcctcccac
      241 gccggcgatg agccccgaca gggcgcccgg catcccggtg aggacgagcg ccatcacgat
      301 cccgaggacg gcgagggcca gcgccgcgat ggcgtaggcc tggccgggcc agggctcgtc
      361 cgcggtcgtt tccccggcgc cgcccagctc ggccagcccc ccgaaggtct ccgagagctc
      421 cggctcggag ccgagggcga tctcccagcc ggtcccggcg gtgacctcga cctcctgacc
      481 cagccctcca gggaacccac cggggaatgg cgtcggcccg gtggtgggca gctcctcccc
      541 gatcacatcc tcgagcgcgc ccgggttgca cgacaccgag acgaagggca ggaagaagca
      601 gaaggccgcg agcgcaaggc ccgcgggcga caagaacctc ctcatagggg gactcctccc
      661 gtcgagtggg tccatttgat gcgatgccgg ggagggggtc atgcatgggg cgcctcaggc
      721 gcccgccacc gtcatctcgc cgacgagcat cgtgggcgtc ccgatcgcgc cccctccggg
      781 gaagaagcga agatccgacc cgatggcgac gagcgccccc aggacgtcct ggagcgtgct
      841 ggcgatcgtc atctcgcgga gcggctctcc gagtgatccc ccctcgatcc ggagccccgt
      901 cgccccgacc gagaactcgc cgctgaccgg gttggcgccg gaatggacgc cggtgacgct
      961 ctggacgtac gcgcccgtgc ccgcccgggc cagcaccgcc tcgggaccgt cctcacccgg
     1021 ctccacgacg aggttcgtgg gggagacccc ggggacactc cggtagcccg ctcgccctgc
     1081 gttccccgtg gacctcgtgc ctcctcgggc cgccgtctgg gcgttgtgga ggaacccgcg
     1141 gagcgtcccg gcctcgatca gcggcgtgcg acccgtcggc acgccctcgt cgtcgaaggg
     1201 agccgagccg ggcccgtcgg ggagccggcc gtcgtccacg agcgtgaggc cctcggcccc
     1261 caccgcctcc ccgaccttgc cggcgaagag cgaccgcccc ttctgcacct cctcggccga
     1321 gagcgcgccc gccagcaccc cgaggaacga ggaggccgcc caggggtcga ggacgacggg
     1381 gagccgctcg gtggagggct tcgtcgcgcc cagcaggcgg gctccccgct cggccgcctc
     1441 ggccgcggcg ccccgccagt ccagctcgtc gagctccctg gccacggcga aggagaaccc
     1501 cgactgcgtc tcaccgtcgc gctcggccag cgccgagacc atgcaccagc agtcggtgcg
     1561 ggtgcgctcg gcccgaacgc ccgcggttga ggcgatcgcc acgcgcgaca cgacgtccgc
     1621 gtagatcacc tcctcgacct tccggacgtc gggatgcgag gaggtcgagg cgcgatcgag
     1681 ctcgagggcg agctccacct tgcgctcggg ccccagcgcg gcctgtgctt cacggaagag
     1741 gcccgagagg ggctcggcgg cctcggcctc ggggaggacg ttcgcctcgt cgggggccgc
     1801 gtggcgggcc gcctcccgag ccgaggccac gaggccggcg acctcgtccc gatccggatc
     1861 cgcggcccac gcgtacccga gccggccgtc cgccatcacc cggacgccga tcccccggct
     1921 ctccgagcgg gccagcgagt cgacctcgcc gccgcgggcc ttcacctcgg tcctgcggga
     1981 ctggccggcg taggcctcga cggcctcgcc gctctccgcg gctcccacgg ccgtcgcgca
     2041 caggtccccg aggtcagcca ccggtccctc cgacggtcat ccgcgcgatc cggagggtgg
     2101 ggctgccggt cgtcaccggc acgccctggc cgtccttgcc gcaggtgccg gggcgggcgt
     2161 cgaagtcgtc cgctacggcg tccaccgccc ggaggatggc gatgccgttg ccgatcaggt
     2221 tcgcgccccg tgccggggtc gtgatcctcc cgtcctcgat caggtaggcc tcggaggccc
     2281 cgaacacgaa gtcgcccgtc gccggattga cctgtccccc gcccagcgcc ttcacgtaca
     2341 ggccccgtgg ggtgtcggcc acgatgtcgc ccgggtcgtc cttccccggg aggatcgagg
     2401 tgttcgtcat ccgggggatc gggaggtgcg cgtaggactg gcggcgggcg ttgcccgtcg
     2461 gcccggcacc gtccttgcgc gcccagaagc ggtcgtagag gaggctcttc aggaccccct
     2521 cctcgaacag gacggtgctc tcagcggggg agccctcgtc gtcgaacgag aacgagcccc
     2581 aggcgtggtc gaccgtggcg tcatcgacgc ccgtgacgat cgtggagccg agcgtctcgc
     2641 cgaggcggcc ccggtagatc gaggcctcct tccccaccgt gtcggcctcc agcccgtgcc
     2701 cgcacgcctc gtggaacagg acgccgcccc cggcggggcc caccacgacc gccatctcgc
     2761 cggccggtgc ggggacgccg tccagcatcg tgatggcctg acgggccgcc ttctccccgg
     2821 tctccgcggg gggatgggcg cgcaggacct ccacgccccc gaggccagcg ggcccctcga
     2881 agcccgtctg gagggcgtcg ccgcgcgccg ccacgacctg cacgacgagg cggagacggt
     2941 ggcgctcctc ctcgacccag aggccctcgg agctcgcgat cagcaggcgc tggaccgagt
     3001 cgccgtagag ggcgatcacc tggcggacct cggggccggc cgcgcgcgcg gcgtcgtcgg
     3061 cctcctggag ccacgccacc ttccgggcgg cggggacgcc gccggtgggc tcctccgggt
     3121 cgtgcgtgac cgcgggctcc gcccgcgtga agtcgagcac cgaggcccgg tccgagccgc
     3181 gggccgcggc agcggctgcc tcggccgccc ccacgagcgc ctcccgatcc aggcggttgg
     3241 agtaggcgta ggcggtggtg tctcccgcca cggcgcgcac gcccgccccc cggtcgtggc
     3301 cgctcgagaa ctcctcgatc ttgccgtcgt ccaggcgtcc ggaggtcgag cgacgatcct
     3361 cggcgaagat ctccgcgaac gcggcgccgc cggccagggc ggcgccgagg acctctcggg
     3421 ccagggaggg atcgagcacg aggccgcatc ctagcaggcg gccctcgcgt gtggcccggc
     3481 cggtgcccag ggggtaccgt ggcggccatg agccgggtct cggccgcccg gcgcgcgccc
     3541 acccgcggct cgcgccgccg gccacgcgca gcgtggccgg cggcgctcac ctgggccggc
     3601 ggcgtcgcgc tcgtcctcct cggcgtcttc ctcgcccacc ccctcctcga ggggcaccgt
     3661 ctgccgctgg gcccggacgg gcccgtctac gtgtggtggt ctcggttcgc ggaggtggag
     3721 ggtctggacg cggtccgccg gcccgggggc cccgccctga tcctggcact cacctcgatg
     3781 ctcgccaccg acccgctccg ggtcgtcgcc gcgctggagc ccatcctagc ggcggcgatc
     3841 gggttggcgg ggggcgcggt cgccgaggcc gccctggggc cggaccgggt ccggttcggg
     3901 acgacctccc tgctcgtcgg ggccttcgcc ggcctgctgg cggccgggtg gattgcgaac
     3961 ctgatgctcg cagcgctctt cctgaccgcc ctggcggcct tcgccgtcgt cgaccggtcc
     4021 tggcgggggg tcgccttcgg atcggcggcc ctggcggccg ccggggtggc gcactccctc
     4081 ttcctcgccg tggcgctggg gatcgtggcg ggcgccgtga tcctcctcgc ccccgacgcc
     4141 ctgcggcgga tccgggccgg cgtccggccc ctcgacacgg cggcgatgcg gatcaccgcg
     4201 ggcgccgccg ccgggggcgg gggcgctctc ctcgcggtgg ggcttctggc cggcgggccg
     4261 accgcgcccg gggagacctc gcaggatcag atcttccgac ggatcgggat gcgaggaacc
     4321 ctcagcgagc ggtaccgcga gcggctgtgg ggggacgccc ggcggatggc gatccccgcc
     4381 gtcgcggcga tcctgggcgc ggcctggccg ggcctgctgg acggcgtgga cggcgagcgc
     4441 cgccgctacg cgatctcggt ggcggcctcg tgggtggccg tcaccgtcgt cggcgtggcg
     4501 gtcctctggg ccaccggcct gggaccgccg ggccggctgc tgaccttcgc ctacgtgctg
     4561 cccctggcgg cggccgcggc ggcggcgagc gcgatgcggc gcggcg
//
LOCUS       DASUTW010000222         6101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_586431, whole genome shotgun sequence.
ACCESSION   DASUTW010000222 DASUTW010000000
VERSION     DASUTW010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586431"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VF058_07560"
     CDS             <1..462
                     /locus_tag="VF058_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208198.1"
                     /translation="GPVTEHRHTDPLDMYYEPPPPPGEKRRTLMLLGLIIGSVVLAAV
                     AQLALKYGMNEVGAIGRSDLGSPVATALRVFRQPAVLGGFALFGLSAVLWLVVLSRTS
                     LSFAYPFAGITYVLILIFDRVVLGEPVTALRWAGVLLIIGGLVLISRTAST"
     gene            459..1346
                     /locus_tag="VF058_07565"
     CDS             459..1346
                     /locus_tag="VF058_07565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6208199.1"
                     /translation="MTADLAVVVVNFNAGEHLRTCVGSVYAAAGDLALDVAVVDNASR
                     DGSAEAAAAANPSIRVIDNPVNRGFAAAANRGFAETEAPFVFLLNPDAAITGGRLDAF
                     VKVAKERPRAGALGCLVRNPDGSLQPSARKVPGIGEGLAHAFLGRVAPDNRWSRSYTM
                     AEWDRASEREVEWVSGSAMLVRREAFHAVGGFDEGYFMYVEDVDLCTRLRKRGWSVLF
                     TPELEVTHEVGVSTRSHPRRMAYEHSRSIYRYFSKHVATGPAIGLRPLARAALWARAA
                     LIARRSPEDRVAGPRRRPR"
     gene            1343..2353
                     /locus_tag="VF058_07570"
     CDS             1343..2353
                     /locus_tag="VF058_07570"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEX6208200.1"
                     /translation="MKAVVLVGGEGTRLRPLTETRPKPLIPFMTRPFLHHLLDLLARH
                     GVEEVVLSSPYLEEEFRAFLKERGGAPRITWITEETPLGTCGAIASAREHLQETFLVC
                     NGDILTDLDLSELVAFHRLRRATGTIALTPVEDARPYGLVETDDEGRILAFREKPSEP
                     IAAHVNAGTYVLEPRALDGVPQGVPVSIERETFPDLIARGEPLYAFPSDAYWRDLGTP
                     ESYLAAHLDALEGRVRDVVAERPHLGPGVVVEPGATVEGTVVLGRGARVARGARVSRS
                     VLHEEALVDQRAEVSGSILGQGSEVGAGAVVEDAVLGDGARVAAGAAVRGERVGPDRS
                     VS"
     gene            2415..4103
                     /locus_tag="VF058_07575"
     CDS             2415..4103
                     /locus_tag="VF058_07575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEX6208201.1"
                     /translation="MTRDPKLAALRAALVAVVAAGLLAAPAGAAKGFRFNGSGWGHGI
                     GLGQYGAFGLAKEGWGPAKIVRHFYTNTSVQRRDPPKGRFRVGLLQGRQSVSVAVQRG
                     RAVLRVGKAVVERIRAGATRRILIVNGRYRVKRPNGSVVKKRLWGSAKKPLEVRLKNG
                     GVVRVPEWGHSAGRGFLRLKIVGKRSAHLVAVVPAEKYLYGLGEVPSSWPMAALKAQA
                     IAARSYAYVVVGFGRTGCACDIYASTRDQAYVGWDKESGTAGGRWVKAVGGTSRKVAV
                     HGGAVIQGLYSSSSGGYTENVENVWGGGAVGYLKGVCDPGDWTTANPNRLWSTSFTPR
                     GLASRLGRPNGIVRVTAVNVNKRGVSGRVVTATVKGKRGNGSTASWSTSGWNLRGRLG
                     LRDTRFWVNQDRQVTGKIREAYDELDCRPGLPKSNQKKISGARWQRFENGRIYHHAGR
                     KRETWLRGSVLDAYLSLGGHGGFLGRPWSYGAQAGGVRARFDGGAIYHHDAVGAHEVH
                     GPVLATYLSHGGPASVLGFPTSDVVSEADGTLVSTFQGGTISCPPDAGCTVTPA"
     gene            4107..5624
                     /locus_tag="VF058_07580"
     CDS             4107..5624
                     /locus_tag="VF058_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEX6208202.1"
                     /translation="MVARRLLPISILAAALSLAPLAGAAERFTFYGSGWGHGIGMSQY
                     GAFGLAKKGWKALRILKHYYTGIGVQRRDPPRDVFRVGLLQYRERITISAQTGPFDLA
                     LSNGTHIETVTQGRTRVITVQSGEFRIETATGQLVGGRLWGGANRHLQLLRADGSVIR
                     VAEWGRRAGRGMLELRIADQGSAHLVAVLDPEEYLYGLGEVPSSWPMAALKAQAIAGR
                     TYAYQRAAGGRTGCACHILGDTRDQAYVGWDKEVGVDGDRWVRAVDETARRVALHGGA
                     PISTLYSSSSGGYTESVENVWGGSPQPYLKGVCDPGDFVSSNPNRTWEVTLSSGDVAA
                     RLRSALGWSVADVRSIKAVRRGVSGRLVEVRVRGRSSGGGSFSRTTSGWSLRGALGLK
                     DTRVWVNADRSVIGAIRKEYDRILCRPGLATSRQRTAAGGVWQRFQRGRILRKGIAAW
                     IRKPILPVYLDRGGPGGPLGYPTSRQKKLKDGRVRARFEGGVITCRPGGGCSVRT"
     gene            complement(5621..>6101)
                     /locus_tag="VF058_07585"
     CDS             complement(5621..>6101)
                     /locus_tag="VF058_07585"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEX6208203.1"
                     /translation="GCGEILTDDAGDLVDEGAGHVDQPPQYQNVPATSGPHFTQWLPP
                     GERVYDVPFDPAFEFRAVHNLEHGYIIMYYEQDGERALDPGIVEDLQTLARSQPKVMI
                     APYPNLQEDENLVLVAWNRAQRCDVSGASAGDVREVAEYFMDQFRGNGGEAPEPNAA"
BASE COUNT          823 a         2122 c         2278 g          878 t
ORIGIN      
        1 ggacctgtga cggagcaccg gcacaccgat ccgctggaca tgtactacga gcctcccccg
       61 cccccggggg agaagaggag gaccttgatg ctgctcggac tgatcatcgg ctcggtcgtc
      121 ctggcggccg tcgcccagct cgccctgaag tacgggatga acgaggtcgg cgcgatcggg
      181 aggtccgacc tcggatcccc cgtggcgacg gccctccgcg tgttccgcca gcccgcggtc
      241 ctcgggggct tcgcgctctt cgggttatcg gccgtcctct ggctggtcgt gctgtcgagg
      301 acctcgctct cgttcgcgta tccgttcgcc gggatcacct acgtgctgat cctgatcttc
      361 gaccgcgtcg tcttggggga gccggtcacg gccctgcgct gggcgggcgt gctcctcatc
      421 atcggcggcc tggtcctcat ctcccggacg gcctccacgt gaccgccgac ctggccgtcg
      481 tcgtggtcaa tttcaacgcc ggcgagcatc tgcggacctg cgtgggctcc gtctatgcgg
      541 ccgcgggcga cctcgccctc gacgtcgcgg tcgtcgacaa cgcctcgagg gacgggagcg
      601 cggaggcggc cgcggcggcg aacccgtcga tccgggtgat cgacaacccg gtgaaccgcg
      661 ggttcgccgc cgcggccaac cgcgggttcg ccgagaccga ggcgccgttc gtcttcctcc
      721 tcaaccccga cgcggccatc accggcggcc ggctggacgc cttcgtcaag gtggcgaagg
      781 agcggccgcg ggcgggcgcg ctgggctgtc tcgtgcgcaa ccccgacggg tcgctccagc
      841 cctccgcccg gaaggtcccc ggaatcggcg aggggctggc ccacgccttc ctgggaaggg
      901 tggccccgga caaccgatgg tcccgctcct acacgatggc cgagtgggac cgggcctccg
      961 agcgggaggt ggagtgggtg tcggggtcgg ccatgctggt ccgccgggag gccttccacg
     1021 ccgtgggcgg gttcgacgag ggctacttca tgtacgtcga agacgtggac ctctgcacgc
     1081 gcctccggaa gcggggatgg tctgtgctgt tcacgcccga gttggaggtg acgcacgagg
     1141 tgggagtctc gacccggagc catccccgcc gcatggccta cgagcactcc cgcagcatct
     1201 accgctactt ctcgaagcac gtcgccaccg ggcccgcgat cggcctgcgg cccctggccc
     1261 gcgcggcgct ctgggcccga gcggcgctga tcgcgcggcg cagccccgag gaccgggtgg
     1321 ccgggccccg gagacggccg cggtgaaggc cgtcgtcctg gtcggtggcg aggggacgcg
     1381 gctccggccg ctcaccgaga cacgccccaa gccgctgatc ccgttcatga ccaggccctt
     1441 cctccaccac ctgctggacc tgctggcccg gcacggggtc gaggaggtgg tcctctcctc
     1501 gccctacctc gaagaggagt tccgggcgtt cctgaaggag cgcggagggg cgccccggat
     1561 cacctggatc accgaggaga cgccgctcgg cacctgtggg gcgatcgcct cggcccgcga
     1621 gcacctccag gagacgttcc tcgtgtgcaa cggcgacatc ctcaccgacc tcgacctctc
     1681 ggagctcgtg gccttccacc ggctgcggag ggcgacgggg acgatcgcgc tcacaccggt
     1741 ggaggacgcc cggccctacg ggctcgtgga gaccgacgac gagggccgga tcctggcgtt
     1801 ccgcgagaag ccgagcgagc cgatcgcggc ccacgtcaac gcggggacgt acgtcctcga
     1861 gccccgcgcg ctcgacggcg tcccccaggg cgtcccggtc tcgatcgagc gcgagacgtt
     1921 cccggacctc atcgcccggg gcgagcctct ctacgcgttc ccctccgacg cctactggcg
     1981 cgacttgggg acgccggagt cgtacctcgc cgcccacctc gacgcgctcg aggggagggt
     2041 ccgggacgtc gtcgcggagc ggccccacct cgggcccggg gtggtggtcg agcccggggc
     2101 cacggttgag gggacggtgg tgctcgggcg gggggctcgc gtggcgcgcg gggcccgcgt
     2161 ctcgcgcagc gtgctccacg aggaggcgct cgtggaccag cgggccgagg tctcgggcag
     2221 catcctcggg caggggagcg aggtgggcgc gggcgccgtg gtcgaggacg cggtcctggg
     2281 cgacggagcc agggtcgccg ccggggccgc cgtccgcggc gagcgcgtag ggccggaccg
     2341 ctcggtctcc tagacatcgg cccccagatg gggctaaatc ggagtgcccg ctccgacgat
     2401 gtgaccaata caccatgacc agggacccga agctcgccgc gctccgagcc gcgctcgtcg
     2461 cggtcgtcgc cgcgggtctc ctggccgccc ctgccggagc ggcgaaaggc ttccgcttca
     2521 acggctcggg gtggggccac gggatcgggc tcggtcagta cggcgcgttc gggctggcca
     2581 aggagggatg gggccccgcg aagatcgtcc gccacttcta cacgaacacg agcgtccagc
     2641 gtcgcgaccc tcccaagggc cggttccggg tggggctcct ccagggccgc cagtcggtct
     2701 cggtcgccgt gcagcggggg agggcggtgc tccgggtggg gaaggccgtc gtggagcgga
     2761 tccgggccgg ggccacgcgc cggatcctca tcgtgaacgg ccggtaccgc gtcaagcgcc
     2821 cgaacggcag cgtggtgaag aagcgcctct gggggtcggc gaagaagccg ctcgaggtcc
     2881 gcctgaagaa cggcggggtg gtccgcgttc ccgagtgggg gcacagcgct gggcgcggct
     2941 tcctccgcct gaagatcgtc ggcaagcgga gcgctcacct ggtggccgtc gtgccggccg
     3001 agaagtacct ctacggcctt ggtgaggtcc cgtcctcgtg gcccatggcc gcgctgaagg
     3061 cccaggccat cgccgcccgc tcctacgcct acgtcgtcgt cggctttggg cgcacgggat
     3121 gtgcgtgcga catctacgcg agcacgcggg accaggcgta cgtcggatgg gacaaggagt
     3181 cgggaacggc cggcgggaga tgggtgaagg ccgtgggcgg cacctcgagg aaggtggcgg
     3241 ttcacggtgg ggcggtgatc caggggctgt actcctcgtc gagcggcggc tacacggaga
     3301 acgtcgagaa cgtctggggc ggcggcgccg tcggctacct gaagggcgtc tgcgatcccg
     3361 gggactggac caccgccaac cccaaccgcc tctggtccac gagcttcacc ccgcggggcc
     3421 tggcatcgcg cctgggccgc ccgaacggga tcgtccgcgt caccgccgtc aacgtgaaca
     3481 agcgcggagt ctccgggcgg gtcgtcacgg ccacggtcaa gggcaagcgg ggcaacggct
     3541 ccaccgcatc gtggagcacg agcgggtgga acctgcgtgg tcggctcggc ctcagggaca
     3601 cgcgcttctg ggtgaatcag gatcggcagg tcacggggaa gatccgcgag gcctacgacg
     3661 agctggactg ccgtcccggg ctcccgaaga gcaaccagaa gaagatcagc ggggcccgct
     3721 ggcagcgctt cgagaacggt cggatctacc accacgccgg gcggaagcgc gagacgtggc
     3781 tgcgcggctc cgtcctcgac gcgtacctct cgctcggggg gcacggcggc ttcctgggac
     3841 ggccctggtc ctacggcgcc caggccgggg gcgtccgcgc gcggttcgac gggggagcga
     3901 tctaccacca cgacgccgtg ggcgcccacg aggtccacgg ccccgtcctc gcgacctacc
     3961 tgagccacgg cggtcccgcc tccgtcctcg gcttcccgac gtccgacgtc gtctcggagg
     4021 ccgacggcac cctcgtctcc accttccagg gcggcacgat ctcgtgcccg cccgacgccg
     4081 gctgcaccgt gacccccgcc tgagcgatgg tcgcccggcg cctcctcccc atctcgatcc
     4141 tggcggcggc tctttcgctc gcgccgctgg cgggcgccgc cgagcgcttc acgttctacg
     4201 gatcgggatg ggggcacggg atcggcatga gccagtacgg agccttcggc ctcgccaaga
     4261 agggctggaa ggccctccgg atcctcaagc actactacac gggcatcggc gtccagcggc
     4321 gcgacccccc tcgcgacgtc ttcagggtgg ggctgctcca gtaccgggag cggatcacca
     4381 tcagcgcaca gaccggcccc ttcgacctgg cgctgtcgaa cggcacgcac atcgagacgg
     4441 tgacccaggg ccgcacgagg gtgatcacgg tccagagcgg cgaattccgg atcgagacgg
     4501 cgaccgggca gctcgtgggc ggccggctct ggggcggggc gaaccggcac ctccagctgc
     4561 tccgagccga cggctcggtg atccgggtgg ccgagtgggg gcgccgggcc ggtcgcggga
     4621 tgctcgagct ccggatcgcc gaccagggca gcgcccacct cgtggccgtg ctcgatcccg
     4681 aggagtacct ctacgggctg ggggaggtcc cctcctcgtg gcccatggcc gccctgaagg
     4741 cccaggccat cgccgggcgc acctacgcgt accagcgggc cgccggcggc cggacggggt
     4801 gtgcgtgcca catcctgggc gacactcggg accaggccta cgtgggatgg gacaaggagg
     4861 tcggcgtcga cggcgaccgc tgggtgcgcg cggtcgacga gacggcccgg cgcgtggcgc
     4921 tccatggggg cgccccgatc tcgacgctct actcgtcctc gagcggcgga tacaccgaga
     4981 gcgtggagaa cgtctggggc ggatcgcccc agccctacct caagggcgtc tgcgatccgg
     5041 gggacttcgt ctcctcgaac cccaaccgga cctgggaggt gacgctctcg tccggcgacg
     5101 tcgccgcgcg tctgcggtcc gccctcggat ggtcggtggc cgacgtccga tcgatcaagg
     5161 cggtgcgacg aggggtctcg ggacggctcg tggaggtccg ggtgcggggt cgctcgtcgg
     5221 gcggagggtc gttctcgagg acaacctcgg ggtggagcct acgcggcgcc ctgggcctca
     5281 aggacacgag ggtgtgggtg aacgccgatc gcagcgtgat cggcgcgatc cgcaaggagt
     5341 acgaccggat cctgtgccga cccgggttgg ccaccagtcg tcagcggacg gccgccggcg
     5401 gtgtgtggca gcggttccag cggggacgga tcctccgcaa ggggatcgcg gcctggatcc
     5461 gcaagccgat cctccccgtc tacctcgatc gggggggtcc cggcgggccg ctcggatatc
     5521 cgacctcccg tcagaagaag ctgaaggacg ggcgcgtgcg ggcgcggttc gagggcgggg
     5581 tgatcacctg ccggccgggc gggggctgct cggtccggac ctaggccgcg ttgggctcgg
     5641 gggcctcgcc cccgttgccg cggaactggt ccatgaagta ctcggcgacc tcgcggacgt
     5701 cgccggcgga ggccccggag acgtcgcacc gctgggcccg gttccaggcc acgagcacga
     5761 ggttctcgtc ctcctggagg ttcgggtagg gagcgatcat caccttgggc tggctgcgag
     5821 ccagggtctg caggtcctcc acgatcccgg gatcgagggc gcgctccccg tcctgctcgt
     5881 agtacatgat gatgtagccg tgctcgaggt tgtggacggc gcggaactcg aacgccgggt
     5941 cgaagggcac gtcgtagacg cgctccccgg ggggcagcca ctgagtgaag tgcggtccgg
     6001 acgtggccgg gacgttctgg tactggggcg gctggtcgac gtggcccgcg ccctcgtcca
     6061 ccaggtcccc ggcgtcgtcg gtgaggatct ccccgcagcc g
//
LOCUS       DASUTW010000223         2544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1882312, whole genome shotgun sequence.
ACCESSION   DASUTW010000223 DASUTW010000000
VERSION     DASUTW010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1882312"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VF058_07590"
     CDS             complement(<1..312)
                     /locus_tag="VF058_07590"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX6208204.1"
                     /translation="MSGRVAYLGMGRMGAAMAATLRREGFDVVVWNRSPEKAEAVAEA
                     SGCEVAATAPEAAGSAEIVLSSLADDAAVQDVYLGSGAVAATSHPLASATASAFSGDR
                     FQ"
     gene            complement(309..1010)
                     /locus_tag="VF058_07595"
     CDS             complement(309..1010)
                     /locus_tag="VF058_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018839527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6208205.1"
                     /translation="MADGNVVVIGGTSGLGREVAAHYAAEGRPVVISGRDEERCSAIA
                     EEIGGDTTAIGLDLHEPEKIGAALEGVGGVDHLVIASIYRDENRVADYDVQAAIRLVT
                     LKLVGYTEVVHALTSRLHDESSILLFGGLAKDRPYPGSTTVTTINAGVVGLVRTFVSE
                     LAPIRTNAIHPGIVADSPFWAAKPPEVLEAVREKTPTGRPLRMQDIVHASVFLLENPA
                     VNGVNLAVDGGWSLT"
     gene            complement(1003..1908)
                     /locus_tag="VF058_07600"
     CDS             complement(1003..1908)
                     /locus_tag="VF058_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6208206.1"
                     /translation="MPRHLVRAASEASYAPPPGFEDHAEGFRRWLVVDEAAGAVHTGF
                     GLGEMDVGGSIAPHLHSYEESLFVLEGEVVLRTPEAAAQMRPGDYGMIPVGVPHALRN
                     ETDGLARWAAMSAPQPRPAHGGDTYWIPPFPEREASPVDPRDPRTRSFGHIDPQNMEV
                     DKQTQDMLAVSASMRTALLVYSGITVKMMVDSDLGAQLSTMFMVQYEPDGVAGPHDHP
                     LEETYLIVEGEVDATFDGERYRMRPGDVAWAGVGCVHSFSNPGPGIVRWLETQAPQPP
                     ARHSYRFARDWDYLKDGLSKEAGRG"
     gene            complement(1911..>2544)
                     /locus_tag="VF058_07605"
     CDS             complement(1911..>2544)
                     /locus_tag="VF058_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6208207.1"
                     /translation="IRTANGGGVRFGGQHSQSVESWAMQIPGLKVVVPSTATDMIGLF
                     AAAVRDPDPVIVIEPKVLYSRKEDVPDGEIVDRLGAAKVLREGGDVTIAALGAMVPQA
                     VDAAERLAGEEIDAEVIDVRSLVPLDATTLLDSVSKTSRLVTVEENPRLCGWGAELAS
                     IVAEERFYDLDGPVVRVTTPHIPLPAADSLEDLAIPSADDVVKRVVEAMG"
BASE COUNT          352 a          955 c          870 g          367 t
ORIGIN      
        1 ttggaaccgg tcaccggaga aagccgaggc ggtggccgaa gccagcgggt gcgaggtggc
       61 ggcgaccgcg cccgagccca ggtagacgtc ctggaccgcc gcgtcgtcgg ccaggctgct
      121 cagcacgatc tccgcgctcc cggccgcctc gggagcggtc gccgccacct cgcacccgct
      181 ggcctcggcc accgcctcgg ccttctccgg ggaccggttc cagaccacca cgtcgaaccc
      241 ctcgcggcgg agagtggcgg ccatggcggc gcccatccgg cccatgccga ggtaggccac
      301 acggccgctc acgtcagcga ccagcccccg tccacggcga ggttgacgcc gttcaccgcg
      361 gggttctcca gcaggaacac cgaggcgtgg acgatgtcct gcatccggag cggacgcccc
      421 gtgggggtct tctcccggac cgcctccagg acctccggcg gcttggccgc ccagaagggg
      481 ctgtcggcca cgatcccggg gtggatcgcg ttcgtgcgga tcggcgccag ctcggacacg
      541 aacgtccgaa ccaaccccac cacgcccgcg ttgatcgtgg tcacggtggt cgagccgggg
      601 tagggcctgt ccttggccag cccgccgaac aggaggatcg agctctcgtc gtggaggcgc
      661 gacgtgagcg cgtgcaccac ctccgtgtag cccacgagct tcagcgtgac cagccggatc
      721 gcggcctgca cgtcgtagtc ggccacgcgg ttctcgtccc gatagatcga ggcgatcacc
      781 agatggtcga caccccccac cccctccagc gccgctccga tcttctccgg ctcgtggagg
      841 tcgagcccga tcgcggtcgt gtcgccgccg atctcctcgg cgatcgccga gcaccgctcc
      901 tcgtcgcgac ccgagatcac gaccggacgg ccctccgccg cgtagtgcgc ggccacctcc
      961 cggcccagcc ccgacgtccc gccgatcacc accacgttgc cgtcagccac gtcctgcctc
     1021 cttcgagagc ccgtccttca ggtagtccca gtcccgggcg aagcggtagg agtgccgcgc
     1081 ggggggctgg ggcgcctggg tctcgagcca gcgaacgatc cccgggcccg ggttcgagaa
     1141 cgaatggacg caccccaccc cggcccaggc cacgtcgccg gggcgcatcc ggtatcgctc
     1201 cccgtcgaac gtcgcgtcga cctcgccctc cacgatcagg taggtctctt ccagcgggtg
     1261 gtcgtggggt ccggccaccc cgtccggctc gtactgcacc atgaacatcg tggagagctg
     1321 cgcgccgaga tccgagtcca ccatcatctt cacggtgatc ccgctgtaga cgaggagggc
     1381 cgtgcgcatg ctggccgaga ccgccagcat gtcctgggtc tgcttgtcga cctccatgtt
     1441 ctggggatcg atgtgcccga aggatcgcgt cctggggtcc cgggggtcca cgggcgaggc
     1501 ctcgcgctcg gggaagggcg ggatccagta ggtgtctccg ccgtgggccg ggcggggctg
     1561 gggcgcggac atcgccgccc accgcgcgag cccgtcggtc tcgttccgga gcgcgtgcgg
     1621 gaccccgacc gggatcatcc cgtagtcgcc cggccgcatc tgtgccgcgg cctcgggcgt
     1681 tcgcagcacc acctcgccct cgaggacgaa caggctctcc tcgtaggagt ggaggtgggg
     1741 ggcgatcgag ccgccgacgt ccatctcgcc gagcccgaag cccgtgtgga ccgcgccggc
     1801 cgcctcgtcc accacgagcc accgacggaa gccctccgcg tggtcctcga aacccggcgg
     1861 cggggcgtag ctggcctcgg acgcggccct gaccaggtga cgcggcatcg tcagcccatc
     1921 gcctcgacga cccgcttgac gacgtcgtcg gccgacggga tcgccaggtc ctcgagggag
     1981 tcggcggcgg gcagggggat gtgcggggtg gtgacgcgga cgaccgggcc gtcgaggtcg
     2041 tagaagcgct cctcggccac gatcgaggcg agctccgccc cccagccgca cagacggggg
     2101 ttctcctcga cggtgacgag ccgggaggtc ttggagaccg agtcgaggag cgtggtggcg
     2161 tccaggggca cgagcgatcg cacgtcaatc acctctgcgt cgatctcctc tcctgcgagc
     2221 cgctcggccg cgtccaccgc ctggggcacc atcgcgccga gcgcggcgat cgtgacgtcc
     2281 ccgccctccc gaagcacctt ggccgccccc agcctgtcca cgatctcgcc gtccgggacg
     2341 tcctccttcc gcgagtagag gaccttgggc tcgatcacga tcaccgggtc ggggtcgcgg
     2401 accgccgcgg cgaacagccc gatcatgtcg gtcgccgtgg agggcacgac gaccttcagg
     2461 ccggggatct gcatcgccca gctctccacg ctctgggagt gctgtccgcc gaagcgcacc
     2521 cctcccccgt tcgccgtccg gatc
//
LOCUS       DASUTW010000224         5136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_456446, whole genome shotgun sequence.
ACCESSION   DASUTW010000224 DASUTW010000000
VERSION     DASUTW010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456446"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..110
                     /locus_tag="VF058_07610"
     tRNA            37..110
                     /locus_tag="VF058_07610"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:71..73,aa:Lys,seq:ctt)
     gene            193..825
                     /locus_tag="VF058_07615"
     CDS             193..825
                     /locus_tag="VF058_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003349336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcI family methyltransferase"
                     /protein_id="HEX6208208.1"
                     /translation="MSVADFHRLYYGAGEHGGTWKDTYWLGVPTWKCPLDLWVYQEIL
                     HETRPDTIVETGTWRGGSALFLASICDLLGRGRVVTIDTEDPGGRPAHDRITYVTGSS
                     TDPGIVREVEGIVGRSRAMVILDSDHSRDHVLGELRAYGPIVSPSHYLVVEDTNINGH
                     PVLPDWGPGPMEAVEEFLRESEDFVVDENREKLLLTFNPRGYLRKVDPGS"
     gene            complement(910..1437)
                     /locus_tag="VF058_07620"
     CDS             complement(910..1437)
                     /locus_tag="VF058_07620"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6208209.1"
                     /translation="MTGFSVQEAREILARTPGVLERLLNEASSGWTHEAPPGAWTPAK
                     ILTHLIHGEKTDWVPRVQIILEEGESRPFEPFDREGGADEAGRPVEKLLAELAELRER
                     NLAYLDGLELDEEALARQGTHPELGTVTLEQLIATWAVHDLGHVGHVVEVMSKRYRET
                     VGPWRAYLPFLDRPD"
     gene            complement(1472..1735)
                     /locus_tag="VF058_07625"
     CDS             complement(1472..1735)
                     /locus_tag="VF058_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208210.1"
                     /translation="MAMIVAGCGEQVETVGTTAECIQKVGAVVDHEFLEERSSSLVQI
                     GAGRGDLVASIAQACVEASPEDPVEEIAERAVKILNPEPLEEK"
     gene            complement(1806..2435)
                     /locus_tag="VF058_07630"
     CDS             complement(1806..2435)
                     /locus_tag="VF058_07630"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX6208211.1"
                     /translation="MVALLEEIVEFIGPLFNGFTGYAIVTAAVLLERSILIGVVVPGE
                     VTMAAGALFAARGQLSLTLIIVLGALAAVVGESIGFWLGRRYGRSLMRRLPLLNRFEH
                     RIEDVEELFDRHGGKAVAVGRFAAGAGVFIPFVAGMGGMRYRRFLLFDVPSIVIWAVG
                     VGFLGYLVGRNIELLDRILTNFGWATLGLLVVGIVAYVFWKRRRESTDD"
     gene            complement(2436..3257)
                     /locus_tag="VF058_07635"
     CDS             complement(2436..3257)
                     /locus_tag="VF058_07635"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX6208212.1"
                     /translation="MAEHRLLSRVIDGVQDRVPKPVGDFIGRLREEDVLLPASGLAFY
                     ALVSMAPLLIVSVWVAGLIVGDARIQQLSQQVKDVAPPGLGLGDLMQRVASQGTTLGV
                     GAVVAALWPATAYGSGLARGFERLHHSDGGEIPGLRGRGLAILVILPMFVLGILGAAF
                     LGTTLLKDGITASILGPVLALVFGFAAAFVGTAVIYWIFPVHPLPWGTLARAAALAAA
                     LIAVLSLLFVLYLNLGADFEESYASSTIAGMVLLGVWLFLSNAVLLAVHAGADER"
     gene            3326..3408
                     /locus_tag="VF058_07640"
     tRNA            3326..3408
                     /locus_tag="VF058_07640"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3359..3361,aa:Leu,seq:tag)
     gene            complement(3374..4258)
                     /locus_tag="VF058_07645"
     CDS             complement(3374..4258)
                     /locus_tag="VF058_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208213.1"
                     /translation="MHEIAERLGATDLFVLYRVAPQRLVNVDGHGRGAGWAGNIDLDP
                     STERWLTAVQEQGLARVSQGMPQRIFGPYWASQAVGVAVGEYVVVLGGRDVANLPDER
                     VRDAAALAVGAVAEVPVAKRLADDLEVTQAALSVARMRPDSLDAAARAIATTAARATS
                     CEFGAVLLFGPPISLHLAEEGWRPSASEEEIAAALLPLAQALSDGLYVEQDTSESPFP
                     YRPLTFEDGLVARCAVRLGEDGDLGVLVVAHSFESPRGFTMLCQRVMRAIGETAQSTL
                     SSLIESGVREGGFEPPRA"
     gene            complement(4251..>5136)
                     /locus_tag="VF058_07650"
     CDS             complement(4251..>5136)
                     /locus_tag="VF058_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6208214.1"
                     /translation="PEPWSSFPRQAHAVDRLPLGGALVVLAAGEGLLDRDVLLRVMGP
                     LDLLVTTMAVMGRQERLEAELLRIRQEQALLAASLQHDLRTPLTSVLGAAQTLLDRSA
                     SLSAEQRAELLEVIAAQAERLNEMIGETIARHDAGPDVPVRMTLVDPAAVARRVCGAA
                     RSARGGEIAIDVEPTPFLCDESRLERALLNLVDNALKYAPDGTEVHVIGARAGGTYTF
                     TVADAGPGVASEVAAAMFSPYVTDPSRDDGTGLGLHSVTHVMEELGGKVSYARASGWT
                     RFSLALPWREPDAGAPDA"
BASE COUNT          816 a         1885 c         1749 g          686 t
ORIGIN      
        1 ggcgggcatg agggcgcccg ctacactgtt cctgcggggc cgttagctca aactggcaga
       61 gcaggggact cttaatccca aggttgaagg ttcgagtcct tcacggccca cgaaagccca
      121 ggtcaggatg gagagcgtcg cccgagagcc cctcccttgc catccggttc ggcgcacgcg
      181 tacgctcgga ccatgtccgt ggccgacttc catcgactgt actacggagc gggggagcac
      241 ggcggaacgt ggaaggacac ctactggctc ggcgtcccca cgtggaagtg cccgctggac
      301 ctctgggtct accaggagat cctccacgag actcgcccgg acacgatcgt cgagacgggc
      361 acgtggcgcg gcgggagcgc cctcttcctc gcctcgatct gcgacctgct ggggcggggg
      421 cgcgtggtca ccatcgacac cgaggatccg ggcggacgcc ccgcacacga tcgcatcacg
      481 tacgtgaccg ggtcatccac cgatccgggg atcgtgcggg aggtcgaggg catcgtcggt
      541 cggtcccgcg cgatggtcat cctcgactcc gaccactcca gggaccacgt cctcggcgag
      601 ctgcgggcct acggcccgat cgtctcgccc tcgcactacc tcgtcgtcga ggacacgaac
      661 atcaacggcc accccgtcct gcccgactgg gggccgggcc cgatggaggc cgtcgaggag
      721 ttcctgcgcg agtccgaaga cttcgtcgtg gacgagaacc gcgagaagct gctgctcacc
      781 ttcaaccccc gaggctacct gaggaaggtc gatccgggct cctgacgatc tcccgttcca
      841 ccgccggcat gctgactcga ctcggggggg aacgctgatc gttcgccccc cagcattccg
      901 ggcccgcgat cagtctgggc ggtcgaggaa gggcaggtac gctcgccagg ggccgaccgt
      961 ctcccggtac cgcttcgaca tgacctcgac gacgtggccc acgtgaccga ggtcgtgaac
     1021 ggcccaggtg gcgatcagct gctccagggt gaccgtgccc agctcgggat gcgtcccctg
     1081 cctggccagc gcctcctcgt ccagctcgag cccgtcgagg tacgcgaggt tccgctccct
     1141 gagctcggcg agctcggcca gcagcttctc gaccggacgg cccgcctcgt ccgccccgcc
     1201 ctcgcggtcg aacggctcga acggcctcga ctcaccctcc tcgaggatga tctggacgcg
     1261 aggcacccag tcggtcttct cgccgtggat caggtgggtg aggatcttgg ccggggtcca
     1321 ggcgccgggc ggcgcctcgt gggtccatcc ggacgaggcc tcgttcagca gccgctccag
     1381 cacccccggc gtgcgggcga ggatctcgcg cgcctcctgc accgagaagc cggtcacgcc
     1441 ccgagcatac cgggccgcgc gctcgacgcc gctacttctc ctcgaggggt tccgggttga
     1501 ggatcttcac ggcccgctcc gcgatctcct ccacgggatc ctcgggggag gcctccacgc
     1561 acgcctgggc gatggaggcc acgaggtcgc ccctccccgc gccgatctgg acgagggagg
     1621 agcttcgctc ctcgaggaac tcgtggtcga cgaccgcgcc caccttctgg atgcactccg
     1681 cggtggtccc cacggtctcc acctgctcgc cgcagccggc gacgatcatc gccacgagca
     1741 gcaggcccag gactctttcc gctcacgctc cctccctcgg tcgaagcggg agggtagcgg
     1801 ctcgctcaat cgtctgtcga ctcgcggcgg cgcttccaga acacgtaggc cacgatcccc
     1861 accacgagga ggcccagcgt cgcccatccg aagttggtga ggatccgatc gaggagctcg
     1921 atgttccgcc cgaccaggta gccgaggaac cccaccccca ccgcccagat gacgatcgat
     1981 gggacgtcga agagcaggaa cctccggtag cgcatgcccc ccatccccgc gacgaacggg
     2041 atgaagaccc cggccccggc cgcgaacctc ccgaccgcca cggccttccc cccgtgccga
     2101 tcgaacagct cctccacgtc ctcgatgcga tgctcgaacc ggttcaggag cgggagtcgc
     2161 ctcatcagcg agcggccgta ccggcgcccc agccagaagc cgatcgactc gccgaccacc
     2221 gcggccaggg cgccgaggac gatgatcagg gtcaggctca gctgccccct ggccgcgaac
     2281 agcgctcccg ccgccatcgt cacctccccc gggaccacga cgccgatcag gatggagcgc
     2341 tcgagcagca cggcggccgt cacgatcgcg tagcccgtga acccgttgaa cagcgggccg
     2401 atgaactcga cgatctcttc gaggagggcg accattcagc gctcgtccgc gcccgcgtgg
     2461 acggcgagga ggaccgcgtt ggagaggaac agccacacgc ccaggaggac catgcccgcg
     2521 atcgtactgc tagcgtagct ctcttcgaag tcggcgccga ggttcagata gaggacgaag
     2581 agcagcgaca ggacggcgat gagagccgcc gccagcgcgg cggcgcgggc cagcgtgccc
     2641 cagggcaggg ggtggacggg gaagatccag tagatcacgg ccgtgccgac gaacgcggcg
     2701 gcgaacccga agaccagtgc cagcacgggc ccgaggatcg acgccgtgat gccgtccttc
     2761 agaagagtcg tgcccaggaa cgccgctccc aggatcccga ggacgaacat ggggaggatc
     2821 accaggatcg ccaggccgcg gccccggagg ccggggatct ccccgccgtc gctgtggtgg
     2881 agacgctcga agcctcgagc cagccccgag ccgtaggcgg tggccggcca cagggcggcc
     2941 accaccgctc cgacccccag ggtcgttccc tgcgaggcca cccgctgcat caggtcgccg
     3001 agtcccaggc cgggaggcgc cacgtccttc acctgctggg acagctgctg gatccgcgca
     3061 tcgcccacga tcaagccggc cacccacacc gagacgatca ggaggggtgc catcgagacg
     3121 agggcgtaga aggccaggcc cgacgcgggc aggaggacgt cctcctcgcg aagcctgccg
     3181 atgaagtctc cgacgggctt cgggacccgg tcctggaccc cgtcgataac cctggagagg
     3241 agcctgtgct cggccaccac cctcggttac ccccgggccc gagcggagaa acgaagcgtg
     3301 cgagacggac cctatactcg ggtgcgcgag agtggcggaa cggcagacgc gccggcctta
     3361 ggagccggtg ggctcaggcc cgtgggggtt cgaatccccc ctctcgcacc ccgctctcga
     3421 tgagcgacga gagggtcgac tgggccgttt cgccgatggc ccgcatcacc cgctggcaga
     3481 gcatcgtgaa gcctcgcggc gactcgaacg agtgggccac caccagcacg ccgaggtcac
     3541 catcctcgcc gagccgcacc gcgcatcgag ccacgagccc gtcctcgaac gtgagcggac
     3601 ggtacgggaa ggggctctcg gacgtgtcct gctcgacgta gagcccgtcg gagagcgctt
     3661 gggccagcgg gaggagggcg gccgcgatct cctcttcgct cgcggagggc cgccagccct
     3721 cctccgcgag atggagcgag atgggaggcc cgaacaggag cacggcgccg aactcgcagg
     3781 aggtggcccg ggccgcggtc gtggcgatgg ccctcgccgc ggcgtccagg gagtccgggc
     3841 gcatccgggc caccgacagc gccgcttggg tcacctcgag gtcgtcggcc aggcgcttcg
     3901 ccactgggac ctcggccacc gccccgacgg cgagggccgc ggcgtcgcgg acccgctcgt
     3961 cgggcaggtt cgccacgtcc cgccccccca gaaccacgac atattccccc accgcgacgc
     4021 ccacggcctg ggacgcccag tagggcccga agatccgctg gggcatcccc tgagagacgc
     4081 gggccaggcc ctgctcctgc accgccgtca gccaccgctc ggtggagggg tcgaggtcga
     4141 tgttcccagc ccacccggca ccccggccgt ggccgtcgac gttcacgagc ctctgggggg
     4201 ccacgcggta gagcacgaag aggtccgtcg ccccgaggcg ctcggcgatc tcatgcatcc
     4261 ggcgctcccg catccggctc ccgccacggg agcgcgagcg agaagcgcgt ccacccgctg
     4321 gcgcgggcgt aggagacctt ccccccgagc tcctccatca cgtgggtgac cgagtggagc
     4381 cccaggccgg ttccgtcgtc ccgggaggga tcggtcacgt agggggagaa catcgcggcg
     4441 gccacctccg aggcgacccc gggcccggca tcggccaccg tgaacgtgta cgtgccgccg
     4501 gcgcgagcgc cgatcacgtg cacctcggtc ccgtccgggg cgtacttgag ggcgttgtcg
     4561 acgaggttga ggagcgctcg ctccagcctc gactcgtcgc agaggaacgg cgtcggttcg
     4621 acgtcgatcg cgatctcgcc acccctcgcc gaccgagcgg cgccgcacac ccgccgagcc
     4681 accgccgccg ggtccaccag cgtcatccgc accggcacgt cggggccggc gtcgtgcctg
     4741 gctatggtct ccccgatcat ctcgttcagg cgctcggcct gcgccgcgat cacctcgagc
     4801 agctccgcgc gctgctccgc cgagaggctc gccgaccgat cgagcagggt ctgcgccgcg
     4861 cccaggacgg aggtcagcgg cgtacggagg tcgtgctgga ggctggcggc cagcagggcc
     4921 tgttcctggc ggatccgcag cagctccgct tcgagccgct cctggcggcc catcacggcc
     4981 atcgtcgtga cgaggaggtc cagggggccc atcacgcgaa ggagcacgtc gcggtcgagg
     5041 aggccttcgc cggccgccag caccacgagg gcgcccccca gcgggaggcg atccacggcg
     5101 tgcgcctggc gggggaagct gctccagggc tccggg
//
LOCUS       DASUTW010000225         3930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1751809, whole genome shotgun sequence.
ACCESSION   DASUTW010000225 DASUTW010000000
VERSION     DASUTW010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1751809"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VF058_07655"
     CDS             <1..208
                     /locus_tag="VF058_07655"
                     /inference="COORDINATES: protein motif:HMM:NF021389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2090 domain-containing protein"
                     /protein_id="HEX6208215.1"
                     /translation="LGRGADDAAVDHWLRQGSGVPGYRGFAIGRTIWGPPLMDFVEGK
                     IDRSEAASRIGRSYRRFIDVYEGA"
     gene            complement(193..657)
                     /locus_tag="VF058_07660"
     CDS             complement(193..657)
                     /locus_tag="VF058_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208216.1"
                     /translation="MARAEEWIGEGAAQYLEPGEEVLAAFVAAPRGYTQSVAGVSGVG
                     EGQMDRASEGADQAGFRLEAPMGIALTPRRLLGLSVSNPVGMGVGGKVKGLLSFAPIE
                     DVDAIEVKRLLMGSRITLTIRGESFKLEAGAGAKAKPLAEAFQQLKAAQAPS"
     gene            complement(766..1809)
                     /locus_tag="VF058_07665"
     CDS             complement(766..1809)
                     /locus_tag="VF058_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX6208217.1"
                     /translation="MKGLTAIPHRVGTVALTDVPEPSQEEGVVLVESLMVGICGTDEE
                     IIAGKYGWAPPGRPRLVLGHEALGRVIDAPPDADVSQGDLVVPFVRHPDPEPCPNCAV
                     GEWDMCRNGRYTEHGIKERDGFLQERYRVDLDRLVRIDPSLGELGVLLEPTSIVAKCW
                     DHVTRIGSRARWHPRRALVTGAGPVGLLAAMLGIQRGLEVHILDRHTDGPKPTLAREV
                     GATYHSDLSQLDTKVDVVVECTGSGALVFETLRRLHPNGVMALAGLAASGRTTTLELD
                     AVNKALVLENLAAFGSVNANRRHYETATAALAAAERGWLRKLITRTVPLERWEEALDE
                     APDDVKVVIRVGS"
     gene            complement(1871..3667)
                     /locus_tag="VF058_07670"
     CDS             complement(1871..3667)
                     /locus_tag="VF058_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX6208218.1"
                     /translation="MPPAIEDYALIGDLHTAGLVNRNGSIDWLCLPRFDSGACFAALL
                     GEPRHGRWSIAPSGRVIRTRRRYRPETLILETEMETDDGAIRLVDCMPPRSSDPTVVR
                     VVEGVRGRVPVHLELVIRFDYGSIVPWVRRVDGALSAIGGPDAVWLRSSVPTKGRDLT
                     TVADFAVDTGQRETFTLTWRASHRRPPPVTDPFHALDETDRWWRRWSQRCTYRGEWRD
                     PVLRSLITLKALTYQPTGGIVAAATTSLPERLGGARNWDYRYCWVRDATFTLYALMLG
                     GYRAEARAWRDWLLRAAAGQPEQLQIMYGPAGERRLSEAELPWLPGYEGSAPVRVGNA
                     ASDQLQLDIYGELMDAMHQARGLGIEPEGPAWDLQRVLMDALESRWTEPDNGIWEVRG
                     PRRHFTHSKVMAWVAADRAVKAVERFGLPGPVERWRRLRNEIWEEVCRKGYNPSRGTF
                     TQSYGSPLLDASLLMIPLVGFLPPTDRRVVDTVEAVERELSRNGLVLRYQTDAPEDVD
                     GLPDGEGAFLPCSFWLADALWLDGRREEARSLFRRLLDLRNDVGLLGEEFDPRAGRML
                     GNFPQAISHVSLVNTALNVGRAPGPMHQRARS"
     gene            3751..>3930
                     /locus_tag="VF058_07675"
     CDS             3751..>3930
                     /locus_tag="VF058_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004095950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX6208219.1"
                     /translation="MAVVKTIELVGVSNESWRDAAAQALAEATKTLRGIEGMDVLETS
                     AVVDGSDISEYHTRVR"
BASE COUNT          583 a         1376 c         1378 g          593 t
ORIGIN      
        1 gctgggacgc ggggccgacg acgccgcggt ggaccactgg ctgcggcagg ggtccggcgt
       61 ccccggctac cggggcttcg ccatcggccg caccatctgg ggacctccgc tcatggactt
      121 cgtggagggc aagatcgacc gcagcgaggc ggcctcgcgg atcggccgga gctaccggcg
      181 cttcatcgac gtctacgagg gggcctgagc cgctttcagc tgctggaacg cctcggccag
      241 gggcttggcc ttggcgccgg cgcccgcctc gagcttgaac gactcgccgc ggatcgtcag
      301 cgtgatgcgt gagcccatga ggaggcgctt cacctcgatc gcgtcgacgt cctcgatcgg
      361 cgcgaagctg agcaggccct tcaccttgcc gccgaccccc atcccgacgg ggttggagac
      421 ggagagcccg aggagccgcc tcggcgtcag cgcgatgccc atcggcgcct cgagtcgaaa
      481 ccccgcctga tcggcgccct cggacgcccg gtccatctgg ccctctccca ccccgctcac
      541 gccggccacc gactgcgtgt agccgcgggg cgccgcgacg aacgcggcca gcacctcctc
      601 gcccggctcc aggtactgcg ccgctccttc gccgatccat tcctcggctc gcgccatgat
      661 ccctccttgc gcaggtgggc aaaggaagcc tatcgagcat ccgagatatg ggccagcgga
      721 cagggggctg taccggaacg gcggaggcgt gtagacgtac gcgactcatg atccgacccg
      781 aatcaccacc ttcacgtcgt cgggggcctc gtcgagcgcc tcctcccatc gctccagggg
      841 aacggtccgg gtgatcagct tgcggagcca cccccgttcc gcggcggcga gggccgcggt
      901 cgccgtctcg tagtggcggc ggttcgcgtt caccgacccg aaagccgcga ggttctccag
      961 gaccaacgcc ttgttcacgg catccagctc gagcgtggtg gttcgcccgc tcgccgcgag
     1021 gccggcgagg gccatcacgc cgttgggatg cagtcgacga agagtctcga agacgagcgc
     1081 ccccgacccc gtgcattcga cgacgacgtc taccttggtg tcgagctgcg agaggtctga
     1141 gtggtaggtg gccccgacct cgcgagccag tgtgggcttt ggtccgtctg tgtgtcgatc
     1201 gaggatgtgg acctcgaggc ctcgttggat cccgagcatc gccgcgagga ggcccacggg
     1261 cccggccccc gtgacgaggg cgcgacgagg gtgccagcgg gcccgggagc cgatcctcgt
     1321 cacgtggtcc cagcatttcg ccacgatgct cgtaggctcc agcagcacgc caagctctcc
     1381 gagcgaagga tcgatgcgca cgagccggtc caggtcgact cgatagcgct cctggaggaa
     1441 gccgtcccgc tccttgatgc cgtgctccgt gtagcggccg ttgcggcaca tatcccactc
     1501 acccacggca cagttcgggc agggctcggg gtcggggtgg cgcacgaagg ggacgacgag
     1561 gtccccttgc gagacgtcgg cgtctggcgg cgcgtcgatc actcggccca gcgcctcgtg
     1621 tcccagcacg agccggggac gaccgggagg cgcccagccg tatttgccag cgatgatctc
     1681 ctcgtcggtc ccgcagatcc ccaccatcag cgactcgacg agcacgaccc cctcctcctg
     1741 tgagggctcc gggacgtccg tgagggcgac ggtgcccaca cgatggggga tcgccgtaag
     1801 ccccttcacg gcgcgggcct ccttctcatc cgctgcctcc ctccgttccg tcacgagcct
     1861 cgatcgtggc tcagctcctg gcgcgctgat gcatcggccc gggggcgcga ccgacgttga
     1921 gcgcggtgtt gacgagcgag acatgggaga tggcctgggg aaagttgccg agcatccgtc
     1981 cggcccgcgg gtcgaattcc tcccccagca ggcccacgtc gttccggagg tcgaggaggc
     2041 gccggaagag ggacctggcc tcctctcgtc tcccatccaa ccacagggcg tcggcgagcc
     2101 agaagctgca gggcaggaac gccccttcac catcggggag cccgtccacg tcctcggggg
     2161 cgtcggtctg gtagcgcagt acgagcccgt tccgcgagag ctctcgctcg acggcttcga
     2221 cggtgtccac gacgcgacgg tcggtggggg gaaggaaccc gacgaggggt atcatcagga
     2281 ggctcgcgtc cagcagtggg gacccgtagg actgagtgaa ggtgccacgg ctcgggttgt
     2341 agcccttgcg gcacacctcc tcccagatct cgttcctgag cctccgccac cgctccaccg
     2401 ggccggggag cccgaaccgc tccaccgcct tcaccgctcg gtccgcggcg acccaggcca
     2461 tgaccttgga atgcgtgaag tggcgccgcg gcccccgcac ctcccagatg ccgttgtcgg
     2521 gctcggtcca gcgcgattcc agcgcatcca tcagcacccg ctgcaggtcc caggccggcc
     2581 cttccggttc gatcccgagg ccccgggcct ggtgcatcgc atccatcagc tcgccgtaga
     2641 tgtcgagctg gagctgatcg gaggccgcgt tccccacccg gacgggcgca gagccctcgt
     2701 agccggggag ccaggggagc tctgcctcgg acaagcggcg ctcgccggcg ggtccgtaca
     2761 tgatctggag ctgctcaggt tggccggccg cggcgcggag cagccagtcc cgccacgctc
     2821 gagcctcggc gcggtagccc ccgagcatca gcgcgtacag ggtgaaggtc gcgtcccgta
     2881 cccagcagta ccggtagtcc cagttgcggg cccccccgag ccgctcgggg agcgaggtgg
     2941 tggcggcggc gacgatcccg ccggtgggct ggtaggtgag cgccttcagc gtgatcagcg
     3001 atcggaggac aggatctcgc cactcgccgc ggtacgtgca gcgctgggac cagcgccgcc
     3061 accatcggtc ggtctcgtcg agcgcatgga aggggtcggt tactgggggc gggcgtcggt
     3121 gcgacgcccg ccaggtcagc gtgaacgtct cccgttgccc tgtgtcgacg gcgaagtcgg
     3181 cgaccgtggt caggtcgcgg cccttcgtgg ggacgctgct gcggagccag acggcgtcgg
     3241 gtccgccgat cgccgacagc gccccgtcca cacggcggac ccaggggacg atcgagccat
     3301 agtcgaatcg gatcacaagc tccagatgca cggggacccg gccccggact ccctccacga
     3361 cgcgcacgac ggtcgggtcg ctgcttctcg gaggcatgca gtcgacgaga cggatcgcgc
     3421 cgtcgtccgt ctccatctcc gtctcgagga tcagcgtctc cgggcggtac cggcgccgcg
     3481 tccggatcac tcggccggac ggagcgatcg accaccgccc gtggcggggc tcgccgagga
     3541 gcgcggcgaa gcaggctccg gaatcgaacc gggggaggca cagccagtcg atcgagccgt
     3601 tccggttcac gagaccggcc gtatggaggt cgccgatcag cgcgtagtcc tcgatcgcgg
     3661 ggggcatggg aaggagtctg ccacgctcgg gttgtccgcg gcatcgtctc cgtcctacga
     3721 tgcgggcgac gctttcccaa ggaggacgcc gtggcggtag tgaagacgat cgagctggtc
     3781 ggggtctcca acgagtcgtg gcgggacgcc gcggcgcagg cgctcgccga ggcgacgaag
     3841 accctccgcg gcatcgaggg gatggacgtg ctggaaacct cggcggtcgt ggacggctcg
     3901 gacatctccg agtaccacac gcgggtgcgg
//
LOCUS       DASUTW010000226         2354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1493836, whole genome shotgun sequence.
ACCESSION   DASUTW010000226 DASUTW010000000
VERSION     DASUTW010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493836"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VF058_07680"
     CDS             complement(<1..342)
                     /locus_tag="VF058_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX6208220.1"
                     /translation="MLRAFIDAFKIPDLRNKILFTLFIIAVYRIGAHWPVPNVDVAAA
                     RSATQVTGGQGNVLSFIDLFAGGALQNFAIFALGIMPYITSSIIMQLLTVVIPKLEQW
                     QKQGEVGVKKIT"
     gene            complement(398..835)
                     /gene="rplO"
                     /locus_tag="VF058_07685"
     CDS             complement(398..835)
                     /gene="rplO"
                     /locus_tag="VF058_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX6208221.1"
                     /translation="MKLHHLKPPKGAKKDRTRVGRGRAGARGKTAGRGTKGYLARNQP
                     KQGFEGGQMPLARRVPKKKGFTNRNREAWVVVNVETLAARFEAGETVTPEAMAERGLV
                     RRRLPVKVLGRGEIDRALTIQAHAVSKAAREKIEGAGGTVEVL"
     gene            complement(832..1014)
                     /gene="rpmD"
                     /locus_tag="VF058_07690"
     CDS             complement(832..1014)
                     /gene="rpmD"
                     /locus_tag="VF058_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019818483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX6208222.1"
                     /translation="MAKLKVTQVRGRAGKPEPQRRTLIALGLRRVRQSVVHEDRPEIR
                     GMIAKVRHLVDVEEVR"
     gene            complement(1001..1585)
                     /gene="rpsE"
                     /locus_tag="VF058_07695"
                     /pseudo
     CDS             complement(1001..1585)
                     /gene="rpsE"
                     /locus_tag="VF058_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514837.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            complement(1597..1962)
                     /gene="rplR"
                     /locus_tag="VF058_07700"
     CDS             complement(1597..1962)
                     /gene="rplR"
                     /locus_tag="VF058_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX6208223.1"
                     /translation="MTTRTAKTRAARMRRHRRVRKRITGTAERPRLAVYRSNRHVYAQ
                     LVDDVSGRTLASASSLEPEVREGGTGKTEVAKVVGGLIAGRAKEAGIERAVFDRGGFL
                     FHGRVAAVAEGAREGGLQI"
     gene            complement(1959..>2354)
                     /gene="rplF"
                     /locus_tag="VF058_07705"
     CDS             complement(1959..>2354)
                     /gene="rplF"
                     /locus_tag="VF058_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX6208224.1"
                     /translation="ERPSDVREHRALHGLTRSLIYNMVHGVTEGYQKVLEIQGVGYRA
                     TLQGTALEVQVGYSHPVRVEAPEGIEFEVPAQNRIVVRGIDKQLVGQVAADIRDIRKP
                     EPYKGKGIRYEGEYVRKKAGKAAKAGLGA"
BASE COUNT          294 a          892 c          771 g          397 t
ORIGIN      
        1 ggtgatcttc ttcaccccga cctcgccctg cttctgccac tgctcgagct tcgggatcac
       61 cacggtgagg agctgcatga tgatcgagct cgtgatgtag ggcatgatcc cgagcgcgaa
      121 gatcgcgaag ttctggagcg cgccaccggc gaagaggtcg atgaacgaga ggacgttccc
      181 ctgccctccc gtgacctgcg tcgcggagcg ggccgcggcc acgtcgacgt tggggacggg
      241 ccagtgcgcg ccgatccggt acacggcgat gatgaagagc gtgaagagga tcttgttccg
      301 gaggtccggg atcttgaacg cgtcgatgaa cgctcgcagc acgtctgctc ctcctcgccg
      361 gcccgcccgg cgaactcgcg gcctagtcta tcggaggcta gaggacttcg accgtcccgc
      421 ccgcgccctc gatcttctcg cgggcggcct tcgagacggc gtgggcctgg atcgtcagcg
      481 cgcggtcgat ctcgccgcgc cccaggacct tcacggggag tcggcgccgg accagccccc
      541 gctccgccat cgcctccggt gtgaccgtct cgcccgcctc gaagcgagcg gccagggtct
      601 cgacgttcac caccacccac gcctcgcggt tccggttcgt gaaccccttc ttcttgggga
      661 ccctgcgggc caggggcatc tgcccgccct cgaacccctg cttgggctgg ttccgagcga
      721 ggtagccctt cgtgcctcgg ccggccgtct tgcctctggc gccggctcgc cctcgaccca
      781 cgcgggtgcg gtccttcttg gcgcccttcg gcggcttgag gtgatgcagc ttcatcggac
      841 ctcctcgacg tccaccaggt ggcggacctt ggcgatcatg cctcggatct ccgggcggtc
      901 ctcgtggacg accgactggc gcacccggcg gaggcccagc gcgatcagcg tccgccgctg
      961 aggctcgggc ttgccggccc gcccccgcac ctgggtgacc ttaagcttcg ccaccggcct
     1021 cctcccgatc cagaccggcc tcccgccgct cgtgcgccag gcgcgtgcga gcggactcca
     1081 tggcctcgat catgggacga ggggcgacct cgtggatctc gcgctcccgc agggcggcca
     1141 cgtcctcggg gcgtcggagc tcgcggagcc ccttcacggt ggcgtgcacg atgttgatcg
     1201 gattgtcgga gcccagggac ttggagagga tgtccttgat ccccgccgcc tcgatcacgg
     1261 cgcgcacggg cccgccggcg atgacgccgg tgccgggcgc ggccggcttg agcaacacgc
     1321 ggcccgcgcc gtcctgaccc accacctggt gcgggatcgt ggtccccatc atcgggacct
     1381 cgaacatggc tcgcttggcc tcctccacgc ccttctgaat cgccgcgggg acctccttag
     1441 ccttcccgta gccgatcccc acgcgtccgg ccgcatcgcc cacgaccacg agggccgcga
     1501 acgagaagcg ccggcccccc ttcacgacct tggccacgcg gttgatcgag accacgcgct
     1561 cctcgtaggg gctcttgtcc tggctgtcgt agcgcctcaa atctggagtc ctccctcccg
     1621 ggccccttcg gccacggccg ccacgcgccc gtggaagagg aagccgccgc ggtcgaagac
     1681 ggcgcgctcg atccccgcct ccttggcccg gccggcgatg aggccgccca cgaccttcgc
     1741 cacttcggtc ttgcccgtcc caccctcccg gacctccggc tcgagcgagg aggccgaggc
     1801 cagcgtgcgg cccgagacgt cgtccacgag ctgggcgtag acgtgacggt tggagcggta
     1861 cacggcgagg cgcggccgct cggcggtgcc ggtgatccgc ttccggacgc gccggtgccg
     1921 gcgcatccgg gcggccctcg tcttcgccgt cctggtcgtc atgcccccag ccccgccttc
     1981 gcggccttcc cggccttctt ccgcacatac tcgccctcgt agcggatgcc cttgcccttg
     2041 tacggctcgg gcttgcggat gtcgcggatg tctgccgcga cctgtcccac gagctgcttg
     2101 tcgatcccgc gcaccacgat gcggttctgg gcggggacct cgaactcgat cccctccggg
     2161 gcctcgacgc ggaccgggtg cgagtacccc acctggacct cgagcgcggt cccctgcagc
     2221 gtcgcccggt agcccacgcc ctggatctcg agcaccttct ggtagccctc ggtgacgccg
     2281 tggaccatgt tgtagatcag cgatcgggtc aggccgtgca gggcccggtg ctcccgcacg
     2341 tccgaggggc gctc
//
LOCUS       DASUTW010000227        20282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_846326, whole genome shotgun sequence.
ACCESSION   DASUTW010000227 DASUTW010000000
VERSION     DASUTW010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 20282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20282
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846326"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1268
                     /locus_tag="VF058_07710"
     CDS             150..1268
                     /locus_tag="VF058_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX6208225.1"
                     /translation="METALLHRAARDPVAMMDECHRRAADASAERLAWIAAVDERRLW
                     RRDGATSMTAWLAARWGLARSTTREMVRVASALRSLPAIREAFEERRLSWDQVRPLTR
                     FATPATDEAWSRRAPRMRPVSLWQEVRRHERLEREEEDSLHRRRYLRMDWDPERPLLH
                     LEGVLASEQGAALQAALGSRAERMETRRDALDPVGAKLADALVGSVTAAPADKAASPV
                     LVVHTAAELLARVPEPGRPHLAETESGVRLSAEAVRRLACHAKVEWVLETDGRPVGIG
                     RRMRAIPPRLWRLLRFRDRACRFPGCERGAWLKAHHIRHWAKGGPTDLDNLVLLCHAH
                     HRLLHEGGWRLRGHPSAELTWIRPDGTRLATSPEVLSS"
     gene            1283..2020
                     /locus_tag="VF058_07715"
     CDS             1283..2020
                     /locus_tag="VF058_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX6208226.1"
                     /translation="MHPSDGLRWTSLAVALAIAGASGPPPERAAAATLAPSGRTGPSD
                     AGPSAAGDRPVSQARFRFEIERVRRADLGRSWRPGCPVPPRQLRLLTVRHWGFDEEVR
                     VGRLVVRRRHARPMIRVMRRLFRVGYPIKRMRPVDRYGGSDRRSMRANNTSAFSCRTA
                     TGSTTWSEHAYGRAIDLNPVQNPYVTSSGRVLPRRGRRFVDRSVDHPAIIRAGDPVVR
                     AFARIGWSWGGAWSSTRDYQHFSSTGR"
     gene            2042..2917
                     /locus_tag="VF058_07720"
     CDS             2042..2917
                     /locus_tag="VF058_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208227.1"
                     /translation="MADDARRAFLHELIDDAGLFPPASLSMDAAAAQHAGARGGPHAW
                     MLGRFICPAGRLSELAGHLGLLDRWRVSVLLDAATLDVGLEASAAFLGVERGRVQIEL
                     VETKLPVTDHESVGDLVEWVEGADLGALPFLEVDPGPGLDDDLEAIAGHAAGAKLRTG
                     GPTEDHVPSPAAVAAFLDGCRRLVLRCKATAGLHHPFRHVDEETGFVQHGFVNVVGAA
                     VLAREHDLGPDRVEAIVAEETPDAFRLSADRFRWRDLDAGPQAVREARGSLLFAYGSC
                     SFQEPVDDLLQLGVL"
     gene            2932..4206
                     /gene="fahA"
                     /locus_tag="VF058_07725"
     CDS             2932..4206
                     /gene="fahA"
                     /locus_tag="VF058_07725"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HEX6208228.1"
                     /translation="MDPGLRSWVPVPEGSDFPIQNLPYGVFSRGGGPARAGVAIGDHV
                     LDLGELALGGLLDGIPGLRPEVFLAEALNPFLGLGRATWRAVRERLSGLLVEGDRRVE
                     EAGLAERTLAPLSEVELHLPFAPGDYVDFYSSIEHATNLGRMFRPEGDPLLPNWRHLP
                     VGYHGRSGTVVVSGTPIRRPVGQTAPAGGGAEPGWGPSRLLDLELEVGFVTGPGNPLG
                     RPIPIDRVADHILGLVLVNDWSARDLQRWEYQPLGPFLGKSFATSISPWVVTLDALAP
                     WRVPPPPQDPEPLPYLRTDEPWGLDVELEVALAPEDAAAPSTISRTNLRGLYWTIAQQ
                     LAHATSNGAVARPGDLFASGTISGSDRGSQGSLIELTEAGRRPLELPGGATRTFLEDG
                     DTVVLRGWCEAGRRRIGFGEVSGRVAPAEPVR"
     gene            4298..5458
                     /locus_tag="VF058_07730"
     CDS             4298..5458
                     /locus_tag="VF058_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEX6208229.1"
                     /translation="MDIEIGIGKQARRGYGFDEVTIVPSRRTRDPDDIDVSWEIDAWT
                     FPLPLMASAMDAVVSPATAIEVGRLGGLAVLNLEGLQTRYEDPDPLFDEVASLPPEKA
                     TRRMQELYQEPVKEELIVRRIKEIKEGGVVSAASLTPQRTARYAHLVLEAELDILVIQ
                     GTVVSAEHVSTQSEPLNLKEFIARFEIPVIVGGCASYSTALHLMRTGAVGVLVGVGPG
                     AACTSRQVLGIGVPQATAIADASAARMQHLHETGRYVHVIADGGMRTGGDIAKAIACG
                     ADAVMIGSPLAKAREAPGQGSHWGMATFHPSLPRGTRVQTRQIGTLEEILVGPAHEND
                     GTLNLFGALRMSMATTGYLNIREFQKAEVMIAPSVATEGKLYQRQQGVGQGA"
     gene            complement(5462..7372)
                     /locus_tag="VF058_07735"
     CDS             complement(5462..7372)
                     /locus_tag="VF058_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX6208230.1"
                     /translation="MDESKGVHMDAKPVQLPELAAKAYGIWADRMLRNLPPSSRRGRI
                     PVIAGGILDQIAAQSSSAGGRGRRITPEISVPLELVDGIVRQLQRLSALTPRKDASPT
                     LEEVGWLIELALRRAARAPSARPASGHPGEALAPEPAPEEDGASVEPAVEEDGASVEP
                     AVEEPPAAAEEQVEVPSTDLQPAAAEEQVEAPSAASEGNGLVEEEPEMVEVDAGSDVL
                     VEPEAPDEEEPAAAGIQGAPNPIRVLLVDDDRRTLAMLEMALKGEHDIEVAGLASDVS
                     KAIEVAGATHPDVAVVDVRMPRGGGPRVARGILALSAETRILAYSALDERRAVLSMLE
                     AGAVGYLVKGGPQEEVAAAIRKAMMGERPMSGEVADEVMKALTERIRLEADDRDSRDE
                     LDRQIRRFLAGRDLRMAWQPIVQLYSRDVVGAEAVPRFGGAPKRTPEGWFREAWRLGF
                     GPELEGTVLEAAAGRLEQLPEELFVAVNLSPGCLVEPGFLASMGHLPWERIVVEVTER
                     SSVDDYAQLRAAAREIRQHGGRLAVDDAGAAFASLRHVAEIEPDLVKMDARVCRNIHI
                     DARMHALATALISFARELGAEIVAEGIEHRGQLDALKDIGVEFGQGPFFTGVDDSKEV
                     QVRVPRKRRARR"
     gene            complement(7425..9083)
                     /locus_tag="VF058_07740"
     CDS             complement(7425..9083)
                     /locus_tag="VF058_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX6208231.1"
                     /translation="MNARGRDRGRASDARAPTPEIILPELPVHTYLRWCDLVRRVAVD
                     RGQATDDVLDAIERQAREAARTHRLILRPRLSLGAEAIAAARERIGRSLTGALDDHDP
                     VAEELEEPTLEIMLVLSREEPGGGTSPTTSSSSSVRETGSIRVLIADDDPRVRKVLAT
                     TLDADELDVVGIAADAEEAIRLAAAERPDVALLDVRMPGGGGGAAARGIAALSPETTI
                     VAHSALEDRQPILDMLRSGAAGYLLKGMDPQDLASAMRSAVRGEPLIAAELVGHVVDE
                     LSQRVRSEARMDGVRENRARQIRRFAAGRGMRMVGQPIYELGSGLPVGIEALARFDSP
                     PHDPEPWFREAWDLGLGPELELAALQRAVAALPSFPEEVYLSVNLSPDTLLSQGFIET
                     KDGLPWERIVLEVTEHVPIADYERFREPVADLRRRGARIAVDDAGAGFASFHHVVELS
                     PDLIKLDRRLTLRIAGDERRRALARALVAFAEEVGTSVVAEGIETGETLRILRRFGVG
                     FGQGHFLATPSSGALRPAALDLALRPAPARIVSDAAPRQRPPTW"
     gene            9259..10806
                     /gene="guaA"
                     /locus_tag="VF058_07745"
     CDS             9259..10806
                     /gene="guaA"
                     /locus_tag="VF058_07745"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX6208232.1"
                     /translation="MPRDASEPRPILVVDFGAQYAQLIARRIRECHVYSEIVSHDATL
                     EEVARRRPAGIVLSGGPASVYEEGAPVLNPHLLEAGVPVLGICYGQQAMAQALDGVVE
                     ATGAREYGKTELRVLEPGVLFREAPVEQTVWMSHSDTVVAQPEGFRVTARTEVSPAAA
                     FEDPERGLYGVQFHPEVSHTPHGPDLLKRFLYDACGLLPDWTPVNVIERAVERVREQV
                     GDAEALCALSGGVDSAVAALLVHRAIGDRLTCVFVDTGLLREGEPEQVEEAFARHFGV
                     PLVHVKAEDRFLARLEGVADPEEKRRRIGEEFIRVFEEVARERRGARFLVQGTLYPDV
                     IESGSSTAATIKTHHNVGGLPERMDLELVEPLRDLFKDEVRVIGAELGLPEEVVHRQP
                     FPGPGLAVRVVGEVTRDRLVLVRAADAIVREEMGRAGLEREIWQAFAVLLDVRSVGVM
                     GDGRTYERPVVLRAVTSEDAMTADWARLPYDVLEAISTRIVREVPGVNRVAYDVTSKP
                     PGTIEWE"
     gene            complement(10807..11481)
                     /locus_tag="VF058_07750"
     CDS             complement(10807..11481)
                     /locus_tag="VF058_07750"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6208233.1"
                     /translation="MGVGWTGAVLSKLRAADARVFDPMRDHYGRLLGRILSGCGSVLD
                     VGCGSDSPLGRLEGRKGHRVGVDIHEGDLQASRRAGIHEAYAVLDVTLLRSAFRPRSF
                     DCVLASDLIEHLEKDAGYELISAMEELARRRVVVFTPNGFLEQPPDPDNPWQEHVSGW
                     TVEELEGLGYRVLGVNGWRPLRTMYAEVRFRPLPLWERLSVLTQPLTEGRPRRAFQLL
                     AMKDLA"
     gene            complement(11577..12428)
                     /locus_tag="VF058_07755"
     CDS             complement(11577..12428)
                     /locus_tag="VF058_07755"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX6208234.1"
                     /translation="MAARRPWTRRARGPSTTGAVVVGVASGFLAGLFGVGGGILIVPG
                     LVFLGMRQRPAHGTSLAAIIPIAAAGIVGFWIEGSVDWPVAAVITVGAATGAFLGTIA
                     LQRLEGRALRLGFAVLLLATAARLFLEVPDRAGRADLDLPFIVGLLLVGLFAGVVAGL
                     FGVGGGVVIVPALVILFSIPDTVAKGTSLVVILPAALVGTARNLRYGNLDVAASVAVG
                     LSGVVTAFLSSKLAIRMDPRLSVILFAGLLVAVTIRLLVVERQRTIARREGRWPPPEE
                     EAGLPEA"
     gene            12483..14630
                     /gene="pcrA"
                     /locus_tag="VF058_07760"
     CDS             12483..14630
                     /gene="pcrA"
                     /locus_tag="VF058_07760"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEX6208235.1"
                     /translation="MDSPILAPLNPVQREAVLHTEGPVLIVAGAGSGKTRILTHRIAY
                     LIKELGVPPGAILAITFTNKAAGEMAERVEELVGSSVARRMWILTFHSTCARILRREH
                     ELLGLPSSFTIYDAADTERVVKMVSGDLGIDEKRFPPRAIAAAIGRLKDAMLSPSDAA
                     ARAANWYEKQVAEVYAEYQRRLQGAGALDFDDLILRTVRLFRDHPEVLAHYQERFRYI
                     LVDEYQDTNRPQYHLVNLLASRYRNLCVVGDADQGIYSWRGATIQNLLDFEHDHPDAA
                     EFVLDQNYRSTRTILEVANALIDHNVQRKPKNLWTDAGGGDPVVRCRAENEHEEAWYV
                     AQEIERLVEEEGFRYSDVAIFYRTNAQSRVLEDVLMKSGILYKVVGGLRFYERREIKD
                     VLAYLKALVNPQDVVATRRVINTPKRGIGDQTLAAVEAFARDEGITVLEAARRAEEIA
                     TLSPRAVGALRGFVEVMDSLRSLLDEGASVARVVEAAYTESGYWATLDAERTVEAESR
                     KENLQELVGVGAEFDVRFPDQGVPDFLEQVSLITEQDEYDETETGVTLMTLHTAKGLE
                     FDVVFIVGMEDGVFPHHRSMTDPAELEEERRLAYVGITRARRRLYLTHAWSRSLFGAS
                     SYNPPSRFLAEVPSELVHEVEGEGRTPREELLPPVPSVEVAAGDTVRHDRFGEGVVVA
                     VTGRGSDAEVTIMFEGEGEKRLLLAYAPLEKVG"
     gene            complement(14635..14952)
                     /locus_tag="VF058_07765"
     CDS             complement(14635..14952)
                     /locus_tag="VF058_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6208236.1"
                     /translation="MTDRVRIERAAPRLVRVSGELDMASSPELAKVLGEIAGAGAGSL
                     VLDLSDLSFIDSTGLRVLIDVAGQLGKSGDLILRNPTRPVMEVLEIAGVAEAAPNFVV
                     ETD"
     gene            complement(15013..15480)
                     /locus_tag="VF058_07770"
     CDS             complement(15013..15480)
                     /locus_tag="VF058_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208237.1"
                     /translation="MVRPRIRFRSPGVKPGQKIGPLGPFRGRLGLQWVIAPIVLGVIL
                     LAAGWVFLRGREPSLPWRPVAEVASLPVGDGRETVPGVFVGRLADGRVIAVAERAGCP
                     LEPVEDGYADCEGAAFGLDGLPKGEGDPLDLVPVRIHDGVLYADPGRRVVRGL"
     gene            15566..15976
                     /locus_tag="VF058_07775"
     CDS             15566..15976
                     /locus_tag="VF058_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020496429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX6208238.1"
                     /translation="MPGKHRILIAKPGLDGHDRGAKVVARALRDAGYEVIYTGLHQTP
                     EQIVEAAIQEDVDAVGLSIHSGAHMTLFPKVATALRERGAGDVVVFGGGIIPKDDVSP
                     LQEAGIERVFTPGAPTSSIVEWLEERLGAGEETS"
     gene            15977..17119
                     /gene="sucC"
                     /locus_tag="VF058_07780"
     CDS             15977..17119
                     /gene="sucC"
                     /locus_tag="VF058_07780"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX6208239.1"
                     /translation="MDLYEHQGKDLFGAHDIALVDGEIATTPDEARTIAERYGGAVAV
                     KVQVQIGGRGKAGGIALVTSADEAEEAARRMLDEGFRGTRVTRVLVERLVDIESQYYM
                     AITLDRSSRRYLAMTSNEGGMEIEDIAKEKPDAIRRSEIDPVLGVMPYQTRYLVGHFP
                     EAARKGSADILDKLYGVLTAADATLVEINPLALLTDGTVVALDAKVTLDDNALFRHPD
                     LAELAKEFVLDPVEARAKELGLQYVKLDGEVGIIGNGAGLVMSTLDVVDQAGGSAANF
                     LDAGGGAGADVMAASLEVILSDPAVRSVLVNIFGGITRGDRVAQGILEAMGRIEVEVP
                     IVVRLDGTNAEEGRRMLAEAEHDRIIPAATMLEAAEKAVAAAKERS"
     gene            17119..17988
                     /gene="sucD"
                     /locus_tag="VF058_07785"
     CDS             17119..17988
                     /gene="sucD"
                     /locus_tag="VF058_07785"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014703068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX6208240.1"
                     /translation="MAVLVTEETRLVVQGITGKEGAFHTLRNRDYGTPIVAGVTPGKG
                     GDSVDGIPVFDSVREAVEETGANTSLTFVPARFAADAILEAADAGVDLVVCITEGIPV
                     QDMARVWNYMLGKDCRLVGPNCPGVISPGKANIGIIPHEITMEGGVGLVSRSGTLTYQ
                     IMNELTQQGIGQSTCIGIGGDPIIGTGFVDALELFEADPETEAIVVIGEIGGDDEEKA
                     AAHIAEHVSKPVVGYVAGFTAPPGKRMGHAGAIITGSKGTAQAKAEAFEEAGVKVGRN
                     PTEVAQIVAAIRS"
     gene            18366..20261
                     /locus_tag="VF058_07790"
     CDS             18366..20261
                     /locus_tag="VF058_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6350 family protein"
                     /protein_id="HEX6208241.1"
                     /translation="MTGEPRLTAGILRALLLGALVFLVMLLLSAVIAFAAFFTSEADV
                     VEVGKVGGVIFFSFHHVGMRTELPTFDPSAVPGAEAAPGGLPLGPIDLSFTIWAALLL
                     GTLLALVLLYRAGRRAAEEGGGSTGAQILQGAFVAVPYAALAALLSLVVRFELDLGPE
                     IASFFGSAGAVEVSAPLLSALLWPLAIAVVAGGLGGYGAARRDVAAATPGRLGAMVSG
                     AWRMLLALLVAGFIGLLILGAVNPDLTASYFETVGSGRSGLVLLLATIAAVPNLALWG
                     ITLGMGGSIVAGDLLGADAVRVASLLEFPTDLGLQLAGGSLVGGTPQVSTEVAPIEYF
                     AFIVLTLIALVIGGRMAAQRARVVSAGQGALIGAGSGVLFAIAFFGGLALAGITVNTS
                     VQAGQEGLARMGPGLMLGGLLGLAWGVVAGAIGGATGTGAEAPFPIERRPAPPPAPSG
                     EGPEATQELPPQPATGTGFEGLGARRAPEPEPEPAPAPEPETEPAPEQEARVEEKEAS
                     APPQAPTEPAPGEPPAFEFTSPQAQWRAPEPEPPAEGEPPAGPEEPAGGSAPGPDQEE
                     PTGEQAPPQTPGWTAPKPDAETPSEAPPWSDPADFEPRPWATEEPEPGPAEGSDDEED
                     EEGPAPA"
BASE COUNT         2795 a         7272 c         7445 g         2770 t
ORIGIN      
        1 gcgaaccgcg ccacggtctt cgtcggcctg atcccgtggg aggagggctc cggcggcccc
       61 tgccgggtga tcgcgcgctg gtaggatcgc cgcgtttcgg gcgggtctga cgagcggttc
      121 atgtcagacc cgcctgctac ggtgcgtcca tggagacggc gcttctgcac cgagccgccc
      181 gggatccggt ggcgatgatg gacgagtgtc accggagggc cgccgacgcc tccgccgagc
      241 ggctcgcgtg gatcgccgcc gtcgacgagc gacgtctctg gcggcgcgac ggggccacct
      301 ccatgaccgc ctggctcgct gcgaggtggg gtctggcccg atcgaccacg cgggagatgg
      361 tgcgggtggc gtcggcgctg cgctcgctgc ccgcgatcag agaggccttc gaggagcgac
      421 ggctctcgtg ggatcaggtc cgtcccctca ccaggttcgc cacgcccgcg accgacgagg
      481 cgtggtcgcg gagggctccc cggatgcgac cggtgagcct ctggcaggag gtgcggcgcc
      541 acgagcgcct ggagcgagag gaggaggact cgctccatcg ccgccggtac ctccggatgg
      601 actgggaccc cgaacgtccc ctgctccacc tcgagggggt gctcgcctcc gagcaggggg
      661 cggcgctcca ggcggcgctg gggagccggg ccgagcggat ggagactcgc cgcgacgcct
      721 tggatcccgt cggggcgaag ctggccgacg cgctcgtggg atccgtcacc gcggctcccg
      781 cggacaaggc ggcgtcgccg gtgctggtcg tgcacacggc ggccgagctc ctggcgcggg
      841 tccccgagcc ggggaggccg cacctggccg agaccgagag cggggtccgg ctgtccgcgg
      901 aggccgtccg caggctggcc tgccacgcga aggtggagtg ggtgctggag acggacggcc
      961 gccccgtggg gatcgggagg cggatgcggg ccatcccccc gcggctgtgg cggctccttc
     1021 gcttccgtga ccgcgcctgc cggttcccgg gatgcgagcg cggggcgtgg ctgaaggccc
     1081 accacatccg ccactgggcg aagggtgggc ccaccgacct ggacaacctc gtgctcctgt
     1141 gccacgcgca ccaccgactg ctgcacgagg gagggtggcg cctccggggc cacccctcgg
     1201 ccgagctgac ctggatccgg cccgacggga cgcggctcgc gacctctccc gaggtcctct
     1261 cgtcctgagg catcatgggg ccgtgcatcc gtcggacggc ctccggtgga cctccctcgc
     1321 ggtcgccctg gccatcgccg gcgcctccgg cccgcccccc gagcgtgccg ccgccgcgac
     1381 gctcgccccc tcgggcagga ccgggccgag cgatgcgggt ccgagcgcgg ccggggaccg
     1441 tcccgtgtcc caggcccgct tccggttcga gatcgagcgg gtgaggcggg cggacctcgg
     1501 gcgctcctgg cggccgggct gcccggtccc gccccggcag ctgcggctcc tcacggtccg
     1561 gcactggggc ttcgacgagg aggtgcgggt cggccgtctg gtcgtgcggc gcaggcacgc
     1621 ccggcccatg atccgggtga tgcggcggct gttccgggtc ggctacccga tcaagcggat
     1681 gcggcccgtg gaccgctacg ggggcagcga ccgccgctcg atgcgggcga acaacacctc
     1741 cgcgttcagc tgccgcaccg ccacgggctc gaccacctgg tcggagcacg cgtacgggcg
     1801 agcgatcgac ctcaaccccg tccagaaccc gtacgtcacc tcgtcgggac gtgtgctgcc
     1861 taggcgggga cgccgcttcg tcgaccggtc cgtggaccac cccgcgatca tccgcgccgg
     1921 ggatccggtg gtccgggcgt tcgcccggat cggttggtcg tggggcggcg cctggagctc
     1981 gacccgagac taccagcact tctccagcac cggacgttga tgcgcgccta gaatcctgcg
     2041 catggcggac gatgctcgac gagccttcct tcacgagctg atcgacgacg ccgggctctt
     2101 ccctcccgcg tcgctgtcga tggatgccgc ggcggcccag cacgcggggg cgcggggagg
     2161 tccgcacgca tggatgctgg gcaggttcat ctgcccggcg gggaggctct cggagctggc
     2221 cggccacctg gggctgctcg atcgatggcg ggtcagcgtc ctgctggacg cggccacgct
     2281 cgacgtgggg ctcgaggcct cggcggcctt cctgggcgtc gagcgcggcc gcgtccagat
     2341 cgagctcgtg gagacgaagc tgccggtcac ggaccacgag tcggtgggcg acctcgtgga
     2401 gtgggtggag ggcgccgacc tgggggccct ccccttcctc gaggtggatc ccggccccgg
     2461 cctggacgac gacctcgaag ccatcgccgg ccacgcggcc ggcgcgaagc tgcgcaccgg
     2521 cggccccacc gaggaccacg tcccctctcc ggccgccgtg gccgcgttcc tggacgggtg
     2581 tcggcggctc gtgctcaggt gcaaggccac cgcggggctc caccatccct tccggcacgt
     2641 ggatgaagag accggcttcg tccagcacgg cttcgtcaac gtcgtcgggg ccgccgtcct
     2701 ggcccgcgag cacgacctcg gccccgatcg ggtggaggcg atcgtggccg aggagacccc
     2761 cgacgccttc cggctctcgg ccgaccggtt ccggtggcgg gacctcgacg ccggacccca
     2821 ggccgtccgg gaggcccggg ggtccctcct cttcgcttac gggagctgca gcttccagga
     2881 gcccgtggac gacctcctcc agctcggggt gctatgagcg acgatccgac ggtggacccg
     2941 ggcctccggt cgtgggtccc ggtccccgag gggtcggact tcccgatcca gaaccttccc
     3001 tacggggtct tctccagggg cgggggcccg gcgcgggcgg gagtggccat cggggaccac
     3061 gtcctggatc tcggggagct cgccctgggc ggcctcctcg acgggatccc cgggctccgg
     3121 cccgaggtct tcctcgcgga ggccctcaac cccttcctcg gcctgggccg ggccacctgg
     3181 cgggcggtgc gcgagcgcct ctcgggcctg ctggtcgagg gggaccgccg ggtcgaggag
     3241 gcggggctcg ccgagcgtac gctggctccc ctctccgagg tcgagctcca cctccccttc
     3301 gctcccggcg actacgtgga cttctactcg tcgatcgagc acgccacgaa cctcgggcgg
     3361 atgttccgcc ccgagggtga tccgctgctc ccgaactggc gccacctccc cgtcggatac
     3421 cacgggcggt cgggaaccgt ggtggtgagc gggacgccga tccggaggcc ggtggggcag
     3481 acggcgccgg ccggtggcgg ggcggagccg ggctggggcc cctcgcgcct cctcgacctc
     3541 gagctggagg tgggcttcgt gaccggcccg ggcaaccccc tcggccggcc gatcccgatc
     3601 gaccgcgtcg ccgatcacat cctcgggctg gtgctcgtca acgactggag cgcccgcgac
     3661 ctccagcggt gggagtacca gccgctcggg ccgttcctgg gaaagtcgtt cgccacctcg
     3721 atctcgccat gggtcgtcac gctcgatgcg ctcgccccct ggagggtccc ccccccgccc
     3781 caggatcccg agccgctccc atacctgcgg acggacgagc cgtgggggct cgacgtggag
     3841 ctggaggtgg cactggcgcc cgaggatgcc gccgcgccct cgacgatctc ccggaccaac
     3901 ctccgaggcc tctactggac gatcgcccag cagctcgcgc acgccacgtc gaacggagcc
     3961 gtggcccgcc cgggcgacct gttcgcctcg gggacgatct ccggttccga ccgcggatcg
     4021 cagggcagcc tgatcgagct cacggaggcg ggcaggcgtc ccctggagct tcccggcggc
     4081 gccacgcgca ccttcctgga ggacggggac accgtcgtcc tgcgcggatg gtgcgaggcg
     4141 gggcgccgcc ggatcgggtt cggcgaggtc tccggccgcg tcgcgcccgc cgagcccgtc
     4201 cgttgaagtc gccgctggcc gctgttaccg tgcgggcatg tgacccgacg cccggccgcg
     4261 ctcatcggcc gggcgtcttc gtctccgagg ggggaacgtg gacatcgaga tcggcatcgg
     4321 gaagcaggct cggcgggggt acgggttcga cgaggtgacg atcgttccct cgcggaggac
     4381 ccgggacccc gatgacatcg acgtctcctg ggagatcgat gcctggacgt tccccctccc
     4441 gctcatggcc tccgccatgg acgcggtggt ctcgccggcc accgcgatcg aggtgggtcg
     4501 cctaggaggc ctcgccgtcc tcaacctcga gggcctgcag acgcgctacg aggaccccga
     4561 cccgctcttc gacgaagtcg cgagcctccc gcccgagaag gcgacgcgcc gcatgcagga
     4621 gctctaccag gagccggtca aagaggagct gatcgtccgc cggatcaagg agatcaagga
     4681 aggtggggtc gtctcggccg cctcgctgac gccgcagcgg acggcacggt acgcgcacct
     4741 cgtcctcgag gccgagctcg acatcctcgt gatccaggga acggtggtct ccgccgagca
     4801 cgtttccacg cagagcgagc cgctcaacct caaggagttc atcgccaggt tcgagattcc
     4861 ggtgatcgtc ggggggtgcg cgtcgtactc gaccgcgctc cacctgatgc ggacgggcgc
     4921 ggtcggcgtg ctggtagggg tggggccggg cgcggcatgc acctcgcgcc aggtcctcgg
     4981 gatcggcgtc ccgcaggcca cggcgatcgc agacgcctcg gccgccagga tgcagcacct
     5041 ccacgagacg gggcgctacg tccacgtgat cgccgacggc gggatgcgga ccggcggcga
     5101 catcgccaag gcgatcgcct gcggggccga cgccgtcatg atcgggtcgc cgctggcgaa
     5161 ggcgcgtgag gcgccgggac agggctccca ctgggggatg gccacgttcc acccgtcgct
     5221 gcctcgaggg acccgggtcc agacccggca gatcgggacg ctggaggaga tcctggtggg
     5281 cccggcccac gagaacgacg gcaccctcaa cctcttcggg gcgctccgga tgtcgatggc
     5341 caccaccggc tacctgaaca tccgggagtt ccagaaggcc gaggtcatga tcgccccctc
     5401 ggtcgccacg gaagggaagc tctaccagcg ccagcagggg gtcggccagg gggcctgagc
     5461 gtcagcggcg ggcccgacgc ttccggggga cgcggacctg gacctccttc gagtcgtcca
     5521 ccccggtgaa gaaggggccc tggccgaact ccacgccgat gtccttgagg gcgtcgagct
     5581 gcccccggtg ctcgatccct tcggccacga tctcggcccc cagctcccgg gcgaacgaga
     5641 tcagggcggt ggccagcgcg tgcatacgcg catcgatgtg gatgttgcga cacacacgcg
     5701 cgtccatctt caccaggtcg ggctcgatct cggcgacgtg ccggaggctg gcgaaggcgg
     5761 ctcccgcgtc gtccacggcg agcctgcccc catgctggcg gatctcccgc gccgccgccc
     5821 ggagctgcgc gtagtcgtcc acggaggagc gctcggtcac ctccacgacg atccgctccc
     5881 aagggaggtg gcccatcgac gccaggaagc ccggctccac aaggcacccc ggggagaggt
     5941 tgaccgcgac gaagagctcc tcgggcagct gctccagccg gccggcggcc gcctcgagca
     6001 cggtcccctc gagctcgggg ccgaacccca gcctccatgc ctcgcggaac catccctcgg
     6061 gcgtgcgctt cggagcgcct ccgaaccggg ggacggcctc ggccccgacg acgtctcgtg
     6121 agtagagctg cacgatcggc tgccaggcca tgcggaggtc ccgcccggcc aggaagcgtc
     6181 ggatctgccg atccagctcg tccctcgagt cgcggtcgtc ggcctccagg cggatccgct
     6241 cggtgagggc cttcatcacc tcgtcggcca cctcccccga catcgggcgc tcgcccatca
     6301 tcgccttgcg gatcgcggcg gccacctcct cctggggccc gcccttcacg aggtagccga
     6361 cggcgccggc ctcgagcatc gacagcaccg ccctgcgctc gtcgagcgcc gagtaggcga
     6421 ggatccttgt ctcggcggag agggccagga tgccgcgcgc tacgcgagga ccgccgcccc
     6481 ggggcatgcg cacgtccacc accgcgacgt ccggatgggt ggcgcccgcc acctcgatcg
     6541 ccttggagac gtcggaggcg aggccggcga cctcgatgtc gtgctccccc ttgagggcca
     6601 tctcgagcat ggcgagtgtc cgacggtcgt cgtcgaccag gaggacccgg atcgggttcg
     6661 gcgctccctg gatgcccgcg gcggcgggct cctcctcgtc cggggcctcc ggctccacga
     6721 gcacgtccga cccggcgtcg acctcgacca tctcgggctc ttcctcgacg aggccatttc
     6781 cttccgacgc agcgctcggg gcctcgacct gctcctcggc ggcggcgggc tgcaggtcgg
     6841 tgctcgggac ctcgacctgc tcctcggccg ccgccggcgg ctcctcgacc gccggctcga
     6901 cagacgcgcc atcctcctcg accgccggct cgacagacgc gccatcctcc tcgggggcgg
     6961 gctccggggc gagggcctcc ccggggtgcc cggaggccgg tcgcgcggat ggggcccgcg
     7021 cggcccggcg cagggcgagc tcgatcagcc agcccacttc ctcgagggtg ggggaggcgt
     7081 ccttccgcgg cgtgagggcg ctgagtcgct gcagctggcg gacgatcccg tccacgagct
     7141 ccaggggtac cgagatctcg ggggtgatcc gccggccgcg gccacccgcg ctcgaggact
     7201 gggccgcgat ctggtcgagg atcccgccgg cgatgacagg gatgcggccg cgccgggagg
     7261 aaggggggag attccgcagc atgcggtcgg cccagatgcc gtaggccttc gcggcgagct
     7321 cgggaagctg aacgggcttg gcgtccatgt gcaccccctt gctctcgtcc atcggcggca
     7381 gggacctcag cggtacagtc ggtcggacgg gtgagatcgg cgcctcacca cgtcggggga
     7441 cgctgtctcg gagccgcgtc cgacacgatc cgtgccggcg cgggcctcag ggcgaggtcg
     7501 agggcggccg gccggagcgc gcccgaagac ggcgtggcca ggaagtgccc ctggccgaag
     7561 cccacgccga accggcggag gatccgaagc gtctctcccg tctcgatccc ctccgctacc
     7621 acggacgtcc ccacctcctc ggcgaacgcg accagcgccc gggccagcgc cctccgccgc
     7681 tcgtccccgg cgatcctcag cgtgagtctt cgatccagct tgatcaggtc gggcgagagc
     7741 tccacgacat gatggaacga ggcgaacccc gctccggcgt cgtccaccgc gattcgggcg
     7801 cccctccgcc gaaggtccgc caccggctct cggaaccgct cgtagtccgc gatcggcacg
     7861 tgctcggtca cctcgaggac gatccgctcc catgggagac cgtccttcgt ctcgatgaat
     7921 ccctgcgaca gcagcgtgtc cggggagagg ttgaccgaca ggtacacctc ctcggggaac
     7981 gaggggaggg cggcgacggc gcgctgcagc gccgccagct ccagctccgg gccgaggccc
     8041 aggtcccagg cctcccggaa ccagggctcg gggtcgtggg gaggcgagtc gaatcgcgcc
     8101 agggcctcga tccccaccgg gaggccgctc cccagctcgt agatcggctg gccgaccatc
     8161 cgcatccccc gtcccgcggc gaaccgcctg atctgacggg cccggttctc ccggaccccg
     8221 tccatcctgg cttccgaccg cacgcgctgc gaaagctcgt ccaccacgtg ccccaccagc
     8281 tcggcggcga tgaggggctc tccgcgaacg gcgctccgca tcgcgctcgc gaggtcctgg
     8341 gggtccatcc ccttgaggag gtagccggcc gcccccgacc gcagcatgtc gaggatgggc
     8401 tgccggtcct cgagggccga gtgggccacg atggtggtct cgggcgagag cgcggcgatg
     8461 ccccgcgccg ccgcgcctcc gccgccgccg ggcatccgca cgtcgagcag ggcgacgtcg
     8521 ggccgctcgg ccgcggccag ccggatcgcc tcctccgcgt cggcggcgat cccgacgaca
     8581 tcgagctcgt ccgcgtccag ggtcgtggcc agcaccttgc gcacccgggg gtcgtcgtcg
     8641 gcgatcagca cgcggatcga acccgtctcc cggacggagg aggaggagga ggtggtgggg
     8701 ctcgtgcctc ctcccggctc ctcccgcgac aggaccagca tgatctccag cgtgggctcc
     8761 tccagctcct cggcgacggg gtcgtggtcg tccagcgccc cagtgagcga tcggccgatc
     8821 cgctcgcggg cggcggcgat cgcctcggct ccgagcgaga ggcgagggcg caggatgagg
     8881 cgatgggtcc gcgcggcctc gcgggcctgc cgctcgatcg cgtcgaggac gtcgtctgtg
     8941 gcttggcccc ggtcgaccgc gacgcgtctc accaggtcgc accagcgcag gtaggtgtgg
     9001 accgggagct ccggcaggat gatctcgggg gtcggcgcgc gcgcatcact cgcgcgtccc
     9061 cggtcgcgtc cgcgtgcgtt catccgacct cgatcgtggg gctctccgcc gccggaggcc
     9121 agtcccgtga tggggggttt ccgggattgc cccgtccgcc ccacgccggt gggccggacg
     9181 accgagggac gcacgtcctg cgtcggatgc ggaacgggca ttgaagcggt gtggtcccgg
     9241 tggtacggtc gcgggaccgt gccccgggac gcctccgagc ctcgaccgat cctcgtcgtc
     9301 gacttcgggg cgcagtacgc acagctcatc gcccggcgga tccgggagtg ccacgtctac
     9361 tcggagatcg tctcccacga cgccaccctc gaggaggtgg ctcggcgtcg accggcgggc
     9421 atcgtgctgt cgggcgggcc cgcctccgtg tatgaggagg gtgcgccggt gctcaaccct
     9481 cacctgctcg aggcgggggt gccggtgctt gggatctgct acggccagca ggccatggcc
     9541 caagccctgg acggcgtggt cgaagccacc ggcgcccggg agtacggcaa gaccgagctc
     9601 cgcgtgctcg agccgggggt cctcttccgc gaggcgccgg tcgagcagac ggtgtggatg
     9661 tcgcacagcg acaccgtggt ggcccagccc gagggcttcc gggtcacggc gcggaccgag
     9721 gtctcgcccg cggccgcctt cgaggatccg gagcgagggc tgtacggcgt gcagttccat
     9781 cccgaggtct cgcacacgcc gcacggcccc gatctcctga agcggttcct gtacgacgcg
     9841 tgtgggctgc tcccggactg gacgccggtg aacgtgatcg agcgcgcggt ggagcgcgtg
     9901 cgcgagcagg tgggcgacgc cgaggctcta tgcgcgctct cgggcggcgt ggactccgcc
     9961 gtggccgccc tcctcgttca ccgggcgatc ggcgaccggc tcacgtgcgt cttcgtggac
    10021 accggcctcc tgcgcgaggg cgagcccgag caggtggagg aggcgttcgc tcgccacttc
    10081 ggggtgcccc tcgtgcacgt gaaggccgag gatcggttcc tggcccggtt ggagggtgtg
    10141 gccgaccccg aggagaagcg gcggcgcatc ggcgaggagt tcatccgggt cttcgaggaa
    10201 gtggcccggg agcgtcgggg ggcgcgcttc ctggtccagg ggaccctcta cccggacgtg
    10261 atcgagtcgg gctccagcac ggctgccacg atcaagacgc accacaacgt gggcgggctt
    10321 ccggagcgga tggacctcga gctcgtggag cccctgcggg acctcttcaa ggacgaggtg
    10381 cgcgtgatcg gcgccgagct ggggctcccc gaggaggtcg tccaccgtca gcccttcccc
    10441 gggcccggcc tggcggtccg ggtggtgggg gaggtgacgc gagaccgcct cgtgctcgtg
    10501 cgggccgcgg acgcgatcgt gcgcgaggag atggggcggg cgggcttgga gcgagagatc
    10561 tggcaggcct tcgcggtcct actcgacgtc cggtccgtgg gtgtcatggg cgacggcagg
    10621 acctacgagc gtcccgtggt gctgcgcgcg gtcacctccg aggacgcgat gaccgccgac
    10681 tgggcccgcc tcccgtacga cgtcctcgag gcgatctcga cccgcatcgt gcgtgaggtc
    10741 ccgggggtga accgcgtggc gtacgacgtc acgtccaagc cccccggcac gatcgagtgg
    10801 gagtgatcag gcgaggtcct tcatcgcgag cagctggaag gcgcggcggg gccgcccctc
    10861 ggtgaggggc tgggtcagga ccgagagccg ctcccacagc gggagcggac ggaacctgac
    10921 ctccgcgtac atcgtgcgaa ggggcctcca cccattgacg cccagcaccc ggtagccgag
    10981 cccctcgagc tcctccaccg tccatcccga gacgtgctcc tgccaggggt tgtccgggtc
    11041 gggcggctgc tcgaggaagc cgttcggcgt gaagacgacc acgcgcctgc gagcaagctc
    11101 ctccatggcc gagatgagct cgtaccccgc gtccttctcc aggtgctcga tgaggtccga
    11161 ggccaggacg cagtcgaagg accgcggccg gaaggcgctc cgcagcaggg tcacgtcgag
    11221 gaccgcgtac gcctcgtgga tgcccgcccg ccgactcgcc tggaggtctc cctcgtggat
    11281 gtccacgccg acccgatggc ccttccttcc ttcgagacgg ccgagggggg agtcgctgcc
    11341 gcagccgacg tcgagcaccg agccgcatcc ggacaggatc cttccgagca ggcgtccgta
    11401 gtggtcacgc atcggatcga acaccctcgc gtccgcggcc cgcagcttcg agagcacggc
    11461 gcccgtccag ccaaccccca cggggggatt cttcctcgct cgccgtccgt cgtccagggg
    11521 ggcggtcggg cggattgcct catcagagcg tttcctgatg gcgtaggagg gtgacctcag
    11581 gcctccggca atccggcctc ctcctcgggc ggcggccacc gcccttccct cctcgcgatc
    11641 gtccgctggc gctccacgac gaggaggcgg atggtcaccg ccacgagcag cccggcgaat
    11701 aggatcaccg agaggcgcgg gtccatccgg atcgcgagct tcgacgagag gaaggccgtg
    11761 accacgccgc tcagcccgac cgccacggag gcggccacgt ccaggttccc gtagcggagg
    11821 ttccgggccg tccccacgag cgcggcgggc aggatcacca cgagcgaggt gcccttggcc
    11881 acggtgtcgg ggatcgagaa caggatcacg agggcgggca cgatcaccac accccctccc
    11941 accccgaaca ggccggcgac cacgcccgcg aacaggccca cgagcagcag gcccacgatg
    12001 aagggcaggt cgagatcggc cctcccggcc cggtcgggaa cctccaggaa gaggcgggcg
    12061 gcggtggcca gcagcagcac ggcgaacccc aaccgaagcg cgcgaccctc cagccgctgg
    12121 agggcgatcg ttccgaggaa cgcccccgtg gcggccccca cggtgatcac ggccgcgacc
    12181 ggccagtcca cggacccctc gatccagaag cccacgatcc ccgcggccgc gatcgggatg
    12241 atcgcggcga gcgaggtccc gtgggccggt cgctgtcgca tccccaggaa caccagcccc
    12301 gggacgatga ggatgcctcc gccgaccccg aagaggcccg ccaggaagcc cgaggccacc
    12361 cccacgacga ccgcgccggt cgtggagggg ccgcgggccc tcctcgtcca gggtcgcctc
    12421 gccgccacgc ggcgccagtg tagtcgcgag gaggggacgg agggcggacc tataatcgcg
    12481 acgtggactc gccgatcctg gccccgctca accccgttca gcgcgaggcc gtcctgcaca
    12541 cggaggggcc ggtgctcatc gtggccgggg caggctcggg gaagacgcgg atcctcaccc
    12601 atcgcatcgc ctacctgatc aaggagctgg gcgtccctcc cggagcgatc ctggcgatca
    12661 ccttcaccaa caaggccgcg ggggagatgg ctgagcgggt cgaggagctc gtgggaagct
    12721 cggtggctcg acggatgtgg atcctcacgt tccactccac gtgcgcccgg atcctgcgga
    12781 gggagcacga gctgctcggg ctcccttcga gcttcacgat ttacgacgcg gccgacaccg
    12841 agcgggtggt gaagatggtc tcgggggatc tggggatcga cgagaagcgc ttcccgcccc
    12901 gtgccatcgc cgcggcgatc ggacggctga aggacgcgat gctctcgccc tcggatgcgg
    12961 cagcgcgggc cgctaactgg tacgagaagc aggtggctga ggtctacgcg gagtaccagc
    13021 ggcggctgca gggggccgga gccctcgact tcgacgacct gatcctccgg acggtccggc
    13081 tgttccgcga ccaccccgag gtcctcgccc actaccagga gcggttccgc tacatcctcg
    13141 tggacgagta ccaggatacg aaccggcccc agtaccacct cgtgaacctg ctggcgtcgc
    13201 ggtaccggaa cctctgcgtc gtgggggacg ccgaccaggg catctactcg tggcggggcg
    13261 ccacgatcca gaacctcctc gacttcgagc acgaccaccc cgacgccgcc gagttcgtcc
    13321 tcgaccagaa ctaccgctcg acgcggacga tcctggaggt ggcgaacgcg ctgatcgacc
    13381 acaacgtcca gcggaagccc aagaacctct ggaccgatgc gggcgggggc gatcccgtcg
    13441 tgcgctgccg ggccgagaac gagcacgagg aggcgtggta cgtggcgcag gagatcgagc
    13501 gcctcgtgga ggaggagggg ttccgctact cggacgtcgc gatcttctac cggaccaacg
    13561 cccagtcccg ggtcctcgag gacgtgctga tgaagtcggg gatcctgtac aaggtcgtgg
    13621 ggggcctccg cttctacgag cgccgcgaga tcaaggacgt cctggcctac ctcaaggcgc
    13681 tggtgaatcc ccaggacgtg gtggccaccc gccgggtgat caacacgccg aagcggggga
    13741 tcggggacca gaccctggcc gcggtcgagg ccttcgcccg ggacgagggg atcacggtgc
    13801 tggaggccgc gcgccgggcc gaggagatcg ccaccctctc cccgcgcgcc gtcggcgccc
    13861 tccgcggctt cgtcgaggtg atggactcgc tgcgctccct cctggacgag ggggccagcg
    13921 tggcgcgggt ggtcgaggcc gcctacacgg aatccgggta ctgggccacg ctcgacgccg
    13981 agcggaccgt ggaggccgaa agccgcaagg agaacctcca ggagctggtg ggggtcgggg
    14041 ccgagttcga cgtccgcttc ccggaccagg gcgtgcccga tttcctggag caggtctcgc
    14101 tcatcaccga gcaggacgag tacgacgaga ccgagaccgg cgtcaccctg atgaccctcc
    14161 acaccgcgaa ggggctggag ttcgacgtgg tcttcatcgt ggggatggag gatggtgtct
    14221 tcccgcacca tcggtcgatg accgaccccg cggagctcga ggaggagcgg cggctggcct
    14281 acgtggggat cacccgggcc cgccgccgcc tgtacctgac ccacgcctgg agccgctcgc
    14341 tgttcggggc atcctcgtac aacccgccct cgcgcttcct ggccgaggtc ccgtccgagc
    14401 tggtacacga ggtcgagggc gagggcagga cgcctcgcga ggagctgctg ccaccggtcc
    14461 ccagcgtcga ggtcgcggcg ggcgacaccg tgcggcacga ccgcttcggc gagggcgtcg
    14521 tcgtggccgt gacgggcagg gggagcgacg ccgaggtgac gatcatgttc gagggcgagg
    14581 gcgagaagcg gctgctcctg gcctacgccc ccctcgagaa ggtgggctga ggcctcagtc
    14641 ggtctccacc acgaagttcg gggccgcctc ggccaccccc gcgatctcca ggacctccat
    14701 cacggggcga gtggggttgc gaagaatcag gtcgccggac ttccccaact gccccgcgac
    14761 gtcgatcagc acacggaggc cggtcgagtc gatgaaggac agatcggaga ggtcgagcac
    14821 cagggagccc gctccggctc ctgcgatctc cccgagcacc ttcgccagct cgggcgacga
    14881 cgccatgtcc agctcgccgg agactcggac gagccgcggc gcggcacgct cgatccgcac
    14941 ccgatcggtc acgagcgggt ccctccctcg ttcgtgcgga ctggttcagt ctaatctgct
    15001 cggcctgctc cgtcagaggc cgcggacgac ccgccggccg gggtccgcgt agaggacccc
    15061 atcgtggatc ctgacgggga cgaggtccag ggggtccccc tcgcccttcg ggaggccgtc
    15121 gagcccgaag gcggcgccct cgcagtccgc gtagccgtcc tcgacgggct cgaggggaca
    15181 gcccgcccgc tcggccaccg cgatcacgcg cccgtcggcc aggcgcccca cgaagacgcc
    15241 gggcacggtc tcgcgaccgt caccgaccgg gagcgaggcg acctccgcca cggggcgcca
    15301 ggggagggag ggctccctgc cgcggaggaa cacccagccc gccgccagga ggatcacgcc
    15361 cagcacgatc ggggcgatca cccactggag ccccagccgt cctcggaagg gcccgagggg
    15421 cccgatcttc tgccccggtt tcaccccggg cgagcggaag cggatccgcg gcctgaccat
    15481 gcccccatca tcccagggtg ggcggcgggc gcccgcacgc atccgctata gttggccgcg
    15541 tctccggagg tcagagggga aacgcatgcc cgggaagcac cggatcctca tcgcgaagcc
    15601 cggactcgac gggcacgacc ggggggcgaa ggtcgtcgcc cgggcccttc gcgacgccgg
    15661 gtacgaggtg atctacaccg gcctccacca gacgcccgag cagatcgtgg aggccgcgat
    15721 ccaggaggac gtggacgcgg tgggcctttc gatccactcg ggcgcccaca tgaccctctt
    15781 ccccaaggtc gcgacggcgc tccgcgagcg cggcgccggc gacgtggtcg tcttcggcgg
    15841 cgggatcatc cccaaggacg acgtctcccc cctccaggag gcggggatcg agcgggtgtt
    15901 cacgccgggg gcgcccacgt cgtcgatcgt ggagtggctc gaggagcggc tgggggccgg
    15961 ggaggagacc tcctaggtgg acctgtacga gcaccagggc aaggacctgt tcggagccca
    16021 cgacatcgcc ctcgtggacg gcgagatcgc caccacgccc gacgaggccc ggacgatcgc
    16081 cgagcgctac gggggcgctg tggcggtgaa ggtccaggtc cagatcgggg gacggggcaa
    16141 ggccgggggc atcgcgctgg tcacgagcgc cgacgaggcc gaggaggccg cccgccgcat
    16201 gctcgacgag ggcttccgcg gcacgcgcgt cacccgcgtc ctcgtggagc ggctcgtcga
    16261 catcgaatcg cagtactaca tggcgatcac actcgatcgg tcgtcccgcc ggtacctggc
    16321 gatgacctcg aacgagggcg ggatggagat cgaggacatc gccaaggaga agcccgacgc
    16381 gatccgccgc tccgagatcg atccggtgct cggggtgatg ccgtaccaga cccggtacct
    16441 cgtggggcac ttccccgagg ccgcgaggaa ggggagcgcc gacatcctcg acaagctcta
    16501 cggggtgctg accgccgcgg acgccacgct cgtggagatc aacccgctgg ccctcctcac
    16561 ggacgggacc gtggtcgccc tcgacgccaa ggtgaccctc gacgacaacg cgttgttccg
    16621 ccatccggac ctcgcggagc tggccaagga gttcgtcctc gaccccgtcg aggccagggc
    16681 caaggagctc ggcctccagt acgtgaagct cgacggcgag gtggggatca tcgggaacgg
    16741 cgccggcctc gtgatgtcca ccctcgatgt tgtggaccag gccggagggt ctgcggccaa
    16801 cttcctggac gcggggggcg gggccggggc cgacgtgatg gccgcgtcgc tggaggtgat
    16861 cctctcggac ccggccgtgc gctcggtcct ggtgaacatc ttcggaggga tcacccgggg
    16921 cgaccgggtg gctcagggca tcctcgaggc gatggggcgc atcgaggtcg aggtccccat
    16981 cgtggtccgc ctggacggga cgaacgcgga ggagggccgc aggatgctgg cggaggcgga
    17041 gcacgaccgc atcatccccg cggccacgat gctggaggcc gccgagaagg ccgtcgcggc
    17101 ggcgaaggag cgctcctgat ggcggtcctc gtcaccgagg agacccggct cgtcgtccag
    17161 ggcatcaccg gcaaggaggg cgccttccac accctccgga accgcgacta cgggacccct
    17221 atcgtggccg gcgtcacccc agggaagggg ggcgactcgg tggacggcat ccccgtcttc
    17281 gacagcgtcc gggaggcggt cgaggagacg ggggccaaca cctcgctcac gttcgtcccg
    17341 gcccggttcg ccgccgacgc gatcctggag gccgccgacg cgggggtgga tctcgtcgtg
    17401 tgcatcaccg agggcatccc ggtccaggac atggccaggg tctggaacta catgctgggg
    17461 aaggattgcc ggctggtggg gccgaactgc cccggcgtca tctcgcccgg gaaggccaac
    17521 atcgggatca tcccccacga gatcaccatg gagggaggcg tggggctcgt ctcccggtcg
    17581 gggacgctga cctaccagat catgaacgag ctgacccagc aggggatcgg ccagtccacc
    17641 tgcatcggca tcggcggcga ccccatcatc ggcacgggat tcgtggacgc actggagctc
    17701 ttcgaggccg atcccgagac cgaggccatc gtggtgatcg gcgagatcgg gggcgacgac
    17761 gaggagaagg ccgccgctca catcgcggag catgtctcga agcccgtcgt gggctacgtg
    17821 gcggggttca cggcaccgcc ggggaagcgg atggggcacg cgggcgcgat catcaccggc
    17881 tccaagggga cggcccaggc caaggccgag gcgttcgagg aggcgggggt gaaggtgggg
    17941 aggaacccca ccgaggtcgc ccagatcgtg gccgcgatcc gaagctgacc ttcccacaac
    18001 ccgtccgggc atcggggcat actgaggggc ggcccgccga ccttgagggg gagaccgtga
    18061 cctgtccgtc gtgcaaccag cccgtaccag agggagctcg gttctgcccg agctgcggag
    18121 ccgcccagcc cgtggctcag gcgccggccg aacccatcga ctcgcaggag gggcctgggg
    18181 gacccggggc gccctcgacc gagcccccgc tcggggcgga gcccccgccc ccgcctcccc
    18241 ccacggagcc accgcccgcg gcggagcagg tggctccgac cccccctccg cccacgcccc
    18301 cgccgagcga ggcgcctccg ccaccgccgc cgggcggcga cggaccggag ccgatgcccc
    18361 gcgcggtgac cggggagccc aggctcaccg ccgggatcct gcgcgcgctc ctcctggggg
    18421 ccctcgtctt cctcgtgatg ctgctcctat ccgccgtgat cgcgttcgcg gccttcttca
    18481 cgagcgaggc cgacgtggtg gaggtgggga aggtcggcgg cgtgatcttc ttctccttcc
    18541 accacgtggg catgcgcacg gagctcccca cgttcgatcc gtcggccgtg cccggtgcgg
    18601 aggccgcccc cggcggcctg ccgctgggcc cgatcgacct ctcgttcacg atctgggcgg
    18661 ccctcctgct ggggaccctg ctggcgctcg tcctgctgta ccgagccggc cgtcgcgccg
    18721 ccgaggaggg gggcggctcg accggggcgc agatcctcca gggggcgttc gtggccgtcc
    18781 cctacgcggc gctggctgcg ctgctctcgc tcgtggtccg gttcgagctc gacctcgggc
    18841 cggagatcgc ctccttcttc gggtcggccg gcgccgtgga ggtgtcggcg ccgctcctct
    18901 cggcgctcct gtggccgctc gccatcgcgg tcgtggccgg gggcctcgga gggtacggcg
    18961 ccgctcgtcg cgacgttgcg gcggcgacgc cggggcggct gggggccatg gtctcgggcg
    19021 cgtggaggat gctcctggcc ctcctcgtgg ccggcttcat cgggctcctg atcctggggg
    19081 cggtcaaccc cgacctgacc gcctcgtact tcgagacggt ggggagcggt cggtcgggcc
    19141 tcgtgctgct gctcgcgacg atcgccgccg tgccgaacct cgccctgtgg ggcatcaccc
    19201 tggggatggg gggcagcatc gtggccggcg atctgctggg cgcggacgcg gtgcgcgttg
    19261 cctccctgct cgagttcccg accgacctgg ggctccagct ggcgggcggt tccctcgtgg
    19321 gggggacgcc tcaggtctcg accgaggtgg cgccgatcga gtacttcgcg ttcatcgtgc
    19381 tgaccctgat cgcgctggtc atcggggggc ggatggcggc tcagcgagcc cgcgtcgtct
    19441 ccgcggggca gggcgcgctc atcggggccg gctcgggggt gctcttcgcc atcgcgttct
    19501 tcgggggcct ggccctcgcg gggatcacgg tgaacacctc ggtccaggcc ggtcaggagg
    19561 ggctcgcccg gatgggcccc gggctgatgc tcggtgggct gctgggcctg gcctggggcg
    19621 tggtggccgg ggccatcggg ggggccacgg ggaccggggc cgaggcgccc ttccccatcg
    19681 agcgacggcc ggcgccgccg cccgcgccct ctggggaagg gccggaggcc acgcaggagc
    19741 tgccgccgca gccggcgacg gggaccgggt tcgaaggcct cggggctcga cgtgcgcccg
    19801 agcccgagcc cgagccggct ccggcgccgg agcctgaaac ggagccggcg ccggagcagg
    19861 aggcccgggt cgaggagaag gaagcgagcg cgccgcccca ggctcctacc gagccggccc
    19921 ccggcgagcc ccccgcgttc gagttcacga gcccgcaggc ccagtggcgg gcccccgagc
    19981 ccgagccccc agccgagggc gagccgcccg ccggcccgga ggagccggcg ggcggctcgg
    20041 cccccgggcc cgatcaggag gagccgacgg gtgagcaggc gccgccgcag acaccggggt
    20101 ggacggcccc gaagccggac gccgagactc cctccgaggc cccgccgtgg agcgatcccg
    20161 cggacttcga gccgcggccg tgggcgaccg aggagccaga gcccggcccc gcggaggggt
    20221 cggatgacga ggaggacgag gagggacccg ccccggcctg agccgggcat gcgagagggc
    20281 cc
//
LOCUS       DASUTW010000228        17979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_717856, whole genome shotgun sequence.
ACCESSION   DASUTW010000228 DASUTW010000000
VERSION     DASUTW010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 17979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17979
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717856"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VF058_07795"
     rRNA            complement(<1..397)
                     /locus_tag="VF058_07795"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(721..2052)
                     /locus_tag="VF058_07800"
     CDS             complement(721..2052)
                     /locus_tag="VF058_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018327004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6208242.1"
                     /translation="MTPKTRYAKSGEIHIAYQMAGSGPPDLVFVPGWVSHLEAAWEEP
                     IYARFLERLASFSRLIMFDKRGTGMSDRDVGLPTLEERMDDVRAVMDTVGSERAAIFG
                     TSEGGNMSLLFAATYPERTVALVTFGVFAKRIRSEDYPWAPTPEERERWYEMLAREWG
                     DPAELATLAPSKVGDGAFAEWWARYLRMGASPRAAQILGRTNTRIDVTHILPTIAVPT
                     LVLHRTGDRDVDVGEARYIADRIPGARLVELPGDDHLINAGDTDAVAGEIEEFLTGVR
                     RGPETDRVLTTILLLDIVGSTDRAAELGDRSWGELLESFRAAVRGELARRRGRELDTA
                     GDGFLAMFDGPARAVRAALAVRDAVGALGLQVRAGLHTGEVEVTGEGVAGIAVHIAAR
                     VMAEAGPSEVLVSRTVTDLVAGSGLGFEDRGERRLKGVPGSWRLYAATGAR"
     gene            complement(2049..3248)
                     /gene="tyrS"
                     /locus_tag="VF058_07805"
     CDS             complement(2049..3248)
                     /gene="tyrS"
                     /locus_tag="VF058_07805"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX6208243.1"
                     /translation="MDPKEQLRILLSGAADVIPVEELEERLASGRPLRVKLGIDPSAP
                     DIHLGHAVVLRTLRRFQDLGHTAVLIIGDFTGLVGDPTGRTETRPALSPQQLEENAQT
                     YLAQVGRVLDVGRAEIRRNGEWLAPLTFADVARLAGAVTVAQLLDRDDFQSRYRKGRA
                     ISLTEFLYPVMQAYDSVAIHADVEMGGTDQTFNLMIGRDMQRAHGQDPQVVFTVPLLT
                     GVDGTRKMSKSYGNHIGVEDPPEEQFGKTMRIPDELLVEWFRLTTDVSPDELEAIERG
                     LADASLHPGEAKRRLAREIVTLYHGVDAAEAVEARFDRVHREGLAPEDLPEVELPEDP
                     RVSLAALIADAGLASSRSQARRLISQGGVRLDGESVDDPRAEMARDALAGKILQVGKR
                     RFARLRA"
     gene            3300..5729
                     /locus_tag="VF058_07810"
     CDS             3300..5729
                     /locus_tag="VF058_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX6208244.1"
                     /translation="MSPPSPRLVRLALAATLLAGACGLPTLEAERARPRPLPQTSFIY
                     ASDGTLLTALHAEQDRVVIGPRRIPRVMKDAVVAVEDQRFWTHGGVDVKAMIRAAAAN
                     VREGAIVQGGSTITQQYVKNTITGDERTVDRKMKEALLAFQLEQEMSKEEILAGYLNT
                     VYFGQGAYGIQQAAREFFSKPARKLNLVESATLAGMIASPATYDPVDNPKRAVRRRND
                     VLKRMYEQGALERDHYLRARTRRLGLRLRPEDAEYPAAHFVEYVKRQILTSPRFGRTY
                     TQRYNFLFGGGLRIRTTIDLEMQAMAEDAIDGILSQPGDPYGALTAVESDTGFIRAMV
                     GGRDFFASPKEGRFAKVNLATGGSTGRQAGSSFKPFALVAALENGISPQQIYQGGTSI
                     ALTDSVCRNSPTDPWVVQNYEGSAYGSVTLEAATVASVNVVYAQVIRDVGPRQVVDAA
                     RRMGIRSPLRPFCSSVLGSNEVNTLEMASAYGTLANLGTHVAPVAIERIEDADGRVIF
                     EANPRETQAVSPAVAWAATDILKGVIERGTGVAAGIGRPAAGKTGTAQQWRDAWFVGF
                     VPQLTAAVWVGFPQGQISMVPPTTRIRVTGGSFPAQIWRSFMLRATQGLPPRDFQQPP
                     SDLVEVAIDVTQGCVATELTPSENIRIVEFIVGTQPTRKCVVARPEITSVPSVIGMTV
                     STAYELLEGAGFAVVQRTEYDPSYAPGTVVAQEPAAGSQSETGIEITITVSTDNPPLV
                     DVPEVVGLYEGKAVTVLEEAGFEVQVVEADRPEKGTRPRKVLAQTPEPGRQRPLGSTV
                     TITINPRPAKQ"
     gene            complement(5704..6294)
                     /locus_tag="VF058_07815"
     CDS             complement(5704..6294)
                     /locus_tag="VF058_07815"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019364243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX6208245.1"
                     /translation="MTRPLPRDFYERPSLALARALLGKVVVSELGGARTVGRIVEAEA
                     YRPDDPASHSFRGPTSGNRTMFGPPGHAYVYVSYGIHRCMNAVARSGSAVLLRALEPL
                     HGLDAMTARRGLEQELLLCAGPGRLCQALGVTRGEDGVDLTTGEGLWITDGPAPRRVV
                     RTPRIGISVAGDRLWRFVEEGSPYASRRATASPAAG"
     gene            complement(6291..8009)
                     /gene="polX"
                     /locus_tag="VF058_07820"
     CDS             complement(6291..8009)
                     /gene="polX"
                     /locus_tag="VF058_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEX6208246.1"
                     /translation="MARSNDEVAAHLQEYSDLLAILGDDSFKSRAYEKAARSVGGYHA
                     EVSTLDAAGCRAIPNVGASVAEKIQEILRTGTFQALEDLRADIPDGVRALLGIPTLGP
                     KKALKLYEELQIASPEELLDALHEQRIRELPGFGPKTEENLLRGIQMLQRSGGRVQIG
                     VAMDLAEELLGHLTEVPQVRRATFAGSLRRMAETIGDIDLLAASEDPVPVMDAFASGP
                     YVREVLAKGETKTSVLTTKGLQVDLRVVEPAVWGAALQYFTGSKAHNVHVREMAVRKG
                     LKLSEYGLFRAESGRLIVAETEEEVYERLGLPWIPPPLREDRGEIEAALGDGLPTLIE
                     RKDVKGDLHTHTDLTDGLATLEEMVAAAERVGYRYCAITDHAELLYMQRMTREKALSQ
                     RERIRELDERSKVTLLHGSELNIQPDGSVDWDEEFLSGFDVLVASIHSHLTMPKAKMT
                     TRMIRAMEHPCVNIIGHPTARQIGRRPPIELDLEEVFKAAARTGTALEVSSFPDRLDL
                     RDEHVLWAKRHGVKFAIDTDAHSTLHLDNLRFGIGTAQRGWLTRDDVINAWPITKLRR
                     FLRKGR"
     gene            complement(8013..8933)
                     /gene="argF"
                     /locus_tag="VF058_07825"
     CDS             complement(8013..8933)
                     /gene="argF"
                     /locus_tag="VF058_07825"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX6208247.1"
                     /translation="MARHFLSMDDLTAEELHGLLDLSRKVKASPEDHATALAGRAVGM
                     IFEKPSTRTRVSFEVGIALMGGHPLALSSSELQLGRGETIEDTGRVLSRYLDVVVLRT
                     FGQERLEALASTSTVPVVNALSDLEHPCQCLADLLTVLEQKGELAGRTLAYLGDGNNV
                     AHSLLLGGAKAGMHVRVATPAGYEPIPQIVERAAEIATETGGSVEVTADPIAAATGVD
                     VLYTDVWASMGQEAEATERVLVFAPYRLDGEKVAVAAEDVFVLHCLPAHRGEEITDEV
                     IDGPHSVVWDQAENRLHAQKALLLRLLGIA"
     gene            complement(8974..11460)
                     /gene="pepN"
                     /locus_tag="VF058_07830"
     CDS             complement(8974..11460)
                     /gene="pepN"
                     /locus_tag="VF058_07830"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEX6208248.1"
                     /translation="MKENLTRDEAAGRAALVRDVAYQIHLDLTGAEQFRCETVIRFVA
                     EPGASTFLDCTAAEVHAAELNGEALPDGAVGEHRIALGGLAERNEVRVVADMAYRAGE
                     HGMTRFEDPVDGAVYLFSDAEPFGAHRIYPCFDQPDVKGTFRIDVTAPASWEVASNMA
                     ADAEPEAEGDARRWSFSESPRMSTYLAAVVAGPFHVARDRRGEIDLGVYCRATLAEHL
                     DPDEILEITKQGFDFFEEAFATPYAFGKYDQVFVPEYAAGAMENPGLVTFNEMHIFRS
                     RVTDARREGRAETILHEMAHMWFGDLVTMRWWDDLWLNETFATFASMLAMERATRFTE
                     PWTSFAHTEKPWASRQDQLPTTHPIVADVPDVESVFLNFDGITYAKGAAVMRQLVAWV
                     GLERFLEGMRVYFERHAFGNAALADFLAVHEESSKRDLGAWADVWLRASGINALTPEV
                     GQGVAILQEGEPLRPHRLAVGAFSPEGEKLVRAHRAELDVTGARTEVPELSSDAPLVL
                     LNDEDLTYAKVRFDGRSLGTLREGLSRIGDGLSRAVCWRALWDMTRDGDWPAREFLAT
                     VLDHVHAETMPSAVQRILGQAATAVSLLGDPANRDAGGSALAGAALQALRAAAADSDL
                     QLIWARTFAAAARGPEHLDLVRALLDGEERIEGLAVDTDVRWHLVRSLASAGRADAGL
                     IAAEQERDPTDEGAREASGARAARPTAEAKAEAWEALTGPEPPRLASARAILGGFQHP
                     EQEEVLAPYARRYFDTLDDAWERFDLSLALSFARGAFPSAIVSEETVAMTDEVLGRDD
                     LPGPIRRVLLEGRDDLLRALRARATDAAAD"
     gene            complement(11462..13837)
                     /gene="pheT"
                     /locus_tag="VF058_07835"
     CDS             complement(11462..13837)
                     /gene="pheT"
                     /locus_tag="VF058_07835"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX6208249.1"
                     /translation="MKVPYSWLRELVPTSLSPEEVAVALTEHGVEVESVLRPWEGLTG
                     VVVARVLEVSDHPRADRLSLAAVDAGGEERRVVVGVRNMGPGDLVPYAPPGATLPGLD
                     GQLERRTIRGETSDGMLCSPKELGISADHGGILILLEGESGEDVAAFLGLDDAVIDIE
                     VFPNRPDLLSVLGVAREVAAITGDELRVPTPSVPEGDEKAAAVASVRVDDPERCPRYL
                     ARVIRGVTVAPSPLRAQIRLTACGMRPVSNVVDATNYALLELGHPMHPFDLRTISGGA
                     IVVRRATDGERLVTLDGVERAFTPEDLMIADPQRAVAVAGVMGGDATEVGDATTDVLL
                     ESAYFDPRGVLRTARRLGLRTEASVRFERGADPEAVADAALHAAGLVAAWAGGTVLSG
                     EIDVGSSPPRRRVAVRPPRASMLLGMEVSAGDILDVMGRLGIEGEEREGAVEVEVPGR
                     RVDLEIEADLIEEVGRVRGYDALPSTLPGVAQAGGLDPEQRALRRIEDALVRTGLMQI
                     HSVSFASTEDLELFEDERAAGVRVANPVSEEQGFLRSSLLPGLLRAAALSIGHRRAAV
                     RLFERGHVMRLEEGVVEEDAVAALLTGPALEAWPAERRPQDVLDAKGALEHLLEELGV
                     DGWSLGDPPGPPYHPGRSARVLLDGEAIGELGELHPSVGERFDVPGRVAVFELRAAPL
                     VAAGAGLVELRPVSRFPPVRRDLAFVVDRSVPAADVRASLVDAAGPLLDRVLLFDVYD
                     GEPLPEGKRSLAFAVDLRAADRTLTDEEAEELVAAIEARLASDVGAELRAG"
     gene            complement(13834..14892)
                     /gene="pheS"
                     /locus_tag="VF058_07840"
     CDS             complement(13834..14892)
                     /gene="pheS"
                     /locus_tag="VF058_07840"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX6208250.1"
                     /translation="MDPAETIADLDRERERGLAAIGEAASLEELRDAEVAVLGRRAPV
                     PAIQRSLGQAAPEDRPRLGKAINEVFESFRQALAERERELMAERERAVAEVDAVDVTL
                     PGRRLPMGALHVLTQTERAMVDAFVALGYRVMEGPEVETDWYNFQALNIPPDHPARTP
                     QDTLYLDVPGASGLLLRTQTSPMQIKAMETQPPPLYVVVPGRTYRNDTLDATHSPVFR
                     QVEGLAVDEGLSLADMKGTLEAWARAMFTPRQRVRLRPDYFPFVEPGAQVDVSCFACD
                     GAGCRICGNGWLEIMGAGMVHPRVLENGGVDPERYTGYAWGIGVERVAMLRHGIPDIR
                     LFYEGDVRFLEQFARGWS"
     gene            14938..15417
                     /locus_tag="VF058_07845"
     CDS             14938..15417
                     /locus_tag="VF058_07845"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6208251.1"
                     /translation="MLGIRWSDADPSGLLIFTMEVEIPRPPEVVWPYLVDWEGLDRWM
                     EEARAFRVLSEAREGVGVEAEATISILGITTRDLVRVTRWEPPAVLEIEHLGWVSGRG
                     LMELGVTAGGSHLFWREALRPPLGPLGRLGMRLMSPVIHRTFRRDLETLRALIERET"
     gene            complement(15414..16832)
                     /locus_tag="VF058_07850"
     CDS             complement(15414..16832)
                     /locus_tag="VF058_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX6208252.1"
                     /translation="MITSTKNPRIVAAAKLKKRAFRDREARFLVEGAQVTREAIDAGR
                     VEVLFHTTDEHGRVDALVDRARESGVRTEAVTQQVVEHLTSTVTPQGIVGVATYVDVP
                     LGELQPDPSLVAVLSSVRDPGNAGTILRTADAAGADGVVFGRESVDVYNPKTVRASAG
                     SLFHLPVARDVDVAEALEALRRGGLQVLATAADGERTIYEADLSPPTALVFGNEAHGL
                     EPEIRAMADATIRVPIRGRAESLNLAASAAVTLFEAVRAREAPGGLAEIAGLIGPAGH
                     DLRSPLTALTGFVTTLGRRWDGFSDEERREIVEGMALDGHRTGALVRLVVDAVRILSG
                     APLTSTSDRADVGAAARWLADLFAASEDFPDVEVEGEARARSDPDRIRALLFGLVDGA
                     VWWGSEGPVSLRVAEEGSYAVVEAWRAGNRQDEEPTEVLGSPEHGGRVSLYAVRLVAE
                     RLGGSLGAETDGGIRLLLRLPA"
     gene            complement(16853..17212)
                     /gene="rplT"
                     /locus_tag="VF058_07855"
     CDS             complement(16853..17212)
                     /gene="rplT"
                     /locus_tag="VF058_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX6208253.1"
                     /translation="MPRVKRGVHGRKKRREILAQAKGYRGARSRRYRVAKEQLLHSGV
                     YAYRDRRRRKGEFRRLWITRIGAASRQHGLSYSRFMTGLKAADVDLDRKVLADIAATD
                     PEGFAGLVEVARRGLGE"
     gene            complement(17212..17406)
                     /gene="rpmI"
                     /locus_tag="VF058_07860"
     CDS             complement(17212..17406)
                     /gene="rpmI"
                     /locus_tag="VF058_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013979589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX6208254.1"
                     /translation="MAKTKTHRGAAKRFRVTRSGKVLHRKANRGHLLGKKSSSRKRRL
                     ARTNEVTAERRTVLRLLGRR"
     gene            complement(17418..17915)
                     /gene="infC"
                     /locus_tag="VF058_07865"
     CDS             complement(17418..17915)
                     /gene="infC"
                     /locus_tag="VF058_07865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX6208255.1"
                     /translation="MNERIRAPQVRLVGPDGGQIGIVSTRDALRQADELDLDLVEVAP
                     QAKPPVARIMDYGKYKYERDVRQKEARKRQARVEVKEIKFRPKIDTHDYETKKGHVVR
                     FLTAGNKVKVTIMFRGREMAHTELGRKILDRLVGDLEDMAQVEQMPKLDGRNMVMVIS
                     PTKKG"
BASE COUNT         2512 a         6656 c         6267 g         2544 t
ORIGIN      
        1 cgtcagggtt tcccccattg cgcaagattc cccactgctg cctcccgtag gagtctgggc
       61 cgtgtctcag tcccagtgtg gctggtcacg ctctcacgcc agctacccgt cgtcgccttg
      121 gtgagccgtt acctcaccaa ctagctgata ggccgcgggc ccctcccagg ccgccggagc
      181 atatcctcgc cggaccatgc gatccagaga gatcatccgg tattagccac ggtttcccgt
      241 ggttatcccg gtgcctgggg caggttgccc acgtgttact cacccgttcg ccgctccgta
      301 cccaggtggg ttacccctcc cgggccgatc gctcgacttg catgtgttag gcacgccgcc
      361 agcgttcgtc ctgagccagg atcaaactct ccgtagagaa cagatccgga ctccgtccgg
      421 agtacatcac catcgccggt ttcggacacg acaaagccgc gtcctccccc gttgacggag
      481 gtacgcactg acttttggca cgctgttcag ttctcaaggt gcgcgccctg gtgggctccg
      541 aagatgagag agtcgccggc ggaggccggc gaccggacct tcgtacgacc attgtatcag
      601 gccgccgtct aggccgtcac ctcggtctgg agtcggtgtc cgttcaagca ggcacccttc
      661 tccgtgggac ccacccgggc cccctgcgag ggcaaaggat actcccccct cgaggggccg
      721 tcaacgggcg ccggtcgccg cgtacaggcg ccaggacccg gggacgccct tgaggcggcg
      781 ctccccccga tcctcgaacc ctagtccgga cccggccacg aggtcggtaa cggtccgcga
      841 caccaggacc tccgagggtc ccgcctcggc catcacgcgg gcggcgatgt ggaccgcgat
      901 gccggcgacg ccctcccccg tgacctccac ctcccccgtg tgcaggccgg cccggacctg
      961 gaggcccagc gcgcccaccg cgtcccggac ggccagcgcc gcgcgcacgg cgcgagccgg
     1021 cccgtcgaac atcgcgagga agccgtcgcc ggccgtgtcc aactcccggc ccctgcggcg
     1081 cgcgagctca cctcgcacgg ccgcccggaa gctctccagc agctcgcccc agctccggtc
     1141 gcccagctca gcagcccggt ccgtggagcc cacgatgtcg aggagcagga tcgtggtgag
     1201 gacgcgatcg gtctccggcc cccgcctcac tcccgtcagg aactcctcga tctcgccggc
     1261 gaccgcatcg gtgtcgccgg cgttgatcag gtgatcgtcc cccggcagct ccacgagccg
     1321 agcccccggg atccggtccg cgatgtaccg ggcctccccc acgtcgacgt cccggtcccc
     1381 ggtccggtgg aggacgagcg tcggcaccgc gatcgtgggg aggatgtggg tcacgtcgat
     1441 gcgggtgttc gtcctgccca ggatctgggc cgcgcggggg ctggccccca tcctgaggta
     1501 ccgggcccac cattccgcaa aggccccgtc ccccaccttg ctcggcgcca gggtggcgag
     1561 ctccgcgggg tcgccccact ctcgggcgag catctcgtac cagcgctctc gctcctccgg
     1621 cgtgggggcc cacgggtagt cctcggaccg gatcctcttg gcgaacacac cgaaggtgac
     1681 cagggccacg gtccgctccg ggtaggtggc cgcgaagagc agcgacatgt tgccgccctc
     1741 cgacgtcccg aagatcgccg cccgctccga acccacggtg tccatcacgg cccgcacgtc
     1801 gtccatccgc tcctcgagcg tgggcagccc gacgtcgcgg tccgacatcc cggtcccccg
     1861 cttgtcgaac atgatcagcc gactgaagga cgcgagccgc tccaggaacc gggcgtagat
     1921 cggctcctcc caggccgcct ccaggtgcga gacccacccc ggcacgaaca cgagatcggg
     1981 cggtcccgat ccggccatct ggtaggcgat gtggatctcg ccgctcttcg cgtaccgcgt
     2041 cttgggggtc acgcccggag cctggcgaag cgtcgcttcc ccacctggag gatcttgccg
     2101 gcgagcgcgt cccgagccat ctcggcccgc gggtcgtcca ccgactctcc gtcgaggcgg
     2161 acgcccccct gggagatcag ccggcgagcc tggctgcggc tggaggccag cccggcgtcg
     2221 gcgatcaggg cggcgagcga tacgcgagga tcctcgggga gctcgacctc ggggaggtct
     2281 tcgggcgcca ggccctcgcg gtggacgcgg tcgaagcggg cctcgaccgc ttcggccgcg
     2341 tccaccccgt ggtagagggt cacgatctcg cgggccaggc gccgcttcgc ctcccccggg
     2401 tggagcgagg cgtccgcgag cccccgctcg atcgcctcca gctcatcggg ggatacatcg
     2461 gtcgtgagcc ggaaccactc cacgagcagc tcgtcgggga tgcgcatcgt cttcccgaac
     2521 tgctcctcgg gcgggtcctc gaccccgatg tgattgccgt aggacttcga catcttccgg
     2581 gtcccgtcga cacccgtcag cagcggaacc gtgaacacga cctggggatc ctggccgtgg
     2641 gcgcgctgca tgtcccggcc gatcatgagg ttgaaggtct gatcggtccc ccccatctcg
     2701 acgtcggcgt ggatggcgac cgagtcgtac gcctgcatca cggggtagag gaactccgtg
     2761 agcgagatcg ccctcccctt gcgatagcgc gactggaaat cgtcccggtc cagcaactga
     2821 gccaccgtga cggccccggc cagacgcgcc acgtcggcga aggtcagcgg cgccagccac
     2881 tcgccgttgc ggcggatctc ggcgcgcccc acgtccagca cccgccccac ctgggccagg
     2941 taggtctggg cgttctcctc gagctgctgg ggcgagagcg cggggcgggt ctcggtccgc
     3001 ccggtcggat cgcccacgag cccggtgaag tccccgatga tgaggacggc ggtgtggccg
     3061 aggtcctgga acctccggag cgtccggagc accacggcat ggccgaggtg gatgtccggg
     3121 gcggaggggt cgatgccgag cttgacgcgg aggggcctcc cgctcgcgag ccgctcctcc
     3181 agctcctcga cggggatcac gtcggcggcg ccggacagca ggatgcgaag ctgctccttg
     3241 ggatccatcc gcgcgcaagg ctaccagggg aaccccaggc ccgggcgtac aatccggcta
     3301 tgagcccccc ttccccgagg ctcgttcgcc tcgccctcgc cgccacgctg ctcgcgggcg
     3361 cctgcggcct cccgacgctg gaggccgagc gggcccgccc caggcccctc ccccagacct
     3421 cgttcatcta cgcgtcggac gggaccctcc tcacggcact ccacgcggag caggaccggg
     3481 tcgtgatcgg gccgaggcgc atcccccggg tgatgaagga cgccgtggtc gcggtggagg
     3541 atcagcgctt ctggacccac ggaggggtgg acgtcaaggc gatgatccgc gcggccgccg
     3601 ccaacgtgcg cgagggggcg atcgtccagg gcggctccac catcacccag cagtacgtga
     3661 agaacaccat caccggggac gagcgtacgg tcgaccggaa gatgaaggag gccctcctcg
     3721 cgttccagct cgagcaggag atgagcaagg aggagatcct cgccggctac ctcaacaccg
     3781 tctacttcgg ccagggcgcc tacgggatcc agcaggcggc ccgtgagttc ttctccaagc
     3841 cggccaggaa gctcaacctc gtggagtcgg cgacgctggc cgggatgatc gccagcccgg
     3901 cgacctacga cccggtggac aacccgaagc gggccgtccg ccgccgaaac gacgtgctga
     3961 agcgcatgta cgagcagggg gcgctggaac gcgaccacta tcttcgggcc aggaccagac
     4021 gtctcggcct caggctccga cccgaggacg cggagtatcc tgccgcgcac ttcgtcgagt
     4081 acgtcaagcg gcagatcctc acgagccccc gcttcggccg cacgtacacc cagcggtaca
     4141 acttcctctt cggaggaggc ctccggatcc gcacgacgat cgacctcgag atgcaggcga
     4201 tggccgagga tgcgatcgac ggcatcctct cccagcccgg cgacccctac ggggcgctga
     4261 ccgccgtgga gtccgacacc ggattcatcc gcgcgatggt cgggggccgg gacttcttcg
     4321 cctctcccaa ggaggggcgg ttcgccaagg tgaacctggc cacggggggc tcgaccgggc
     4381 ggcaggcggg atcctcgttc aagccgttcg ccctcgtggc cgccctggag aacggcatct
     4441 cgccccagca gatctaccaa ggagggacct cgatcgcgct gaccgactcg gtctgccgga
     4501 actcccccac cgacccctgg gtggtccaga actacgaggg ctcggcctac gggagcgtga
     4561 cgctggaggc ggccacggtg gcgagcgtca acgtcgtcta cgcccaggtg atccgcgacg
     4621 tcgggcctcg ccaggtggtg gacgccgccc ggaggatggg gatccgcagc cccctccgcc
     4681 ccttctgctc atcggtgctg ggctcgaacg aggtgaatac gctggagatg gcctccgcct
     4741 acggcacgct cgcgaacctc gggacccacg tcgcccccgt ggccatcgag cgcatcgagg
     4801 acgcggacgg gcgcgtgatc ttcgaggcga acccgaggga gacgcaggcc gtgagccccg
     4861 ccgtggcctg ggcggccacc gacatcctga agggcgtgat cgagcgcggg acgggggtcg
     4921 ccgcgggcat cgggcgcccg gcggccggga agacggggac cgcccagcag tggcgcgacg
     4981 cgtggttcgt cggcttcgtg ccccagctca cagccgccgt ctgggtgggg ttcccccagg
     5041 gccagatctc gatggtcccc ccgaccacca ggatccgcgt cacgggggga tccttccccg
     5101 cgcagatctg gcgttcgttc atgctgcggg ccacccaggg cctccctccc cgggacttcc
     5161 agcagccgcc ctccgacctg gtcgaggtgg cgatcgacgt gacccaaggg tgcgtcgcca
     5221 cggagctgac gccctcggag aacatccgca tcgtggagtt catcgtgggg acccagccca
     5281 cgcggaagtg cgtcgtggcc cgtcccgaga tcacgagcgt cccgtcggtg atcgggatga
     5341 cggtcagcac ggcctacgag ctgctcgagg gggctggctt cgcggtggtg cagcggaccg
     5401 aatacgaccc gagctacgct cccgggaccg tggtcgcgca ggagccggcg gccgggagtc
     5461 agtcggaaac aggcatcgag atcacgatca ccgtctccac cgacaacccg ccgctcgtgg
     5521 acgtacccga ggtggtcggg ctctacgagg gaaaggccgt gaccgtgctt gaggaggccg
     5581 ggttcgaggt gcaggtggtc gaggccgacc gaccggagaa ggggacgagg ccgaggaagg
     5641 tgctggccca gaccccggag ccgggcaggc agcgcccact cggctcgacc gtaacgatca
     5701 cgatcaaccc gcggccggcg aagcagtagc ccgccgcgag gcgtaggggc tcccctcctc
     5761 cacgaaccgc cagagcctgt cgccggcgac gctgatcccg atgcggggcg tccgcaccac
     5821 ccgccgcggg gccggaccgt cggtgatcca cagcccctcg ccggtcgtca ggtcgacgcc
     5881 gtcctcgcct cgcgtgacgc cgagggcctg gcacaggcgg ccggggcccg cgcacagcag
     5941 cagctcctgc tccaggccgc ggcgggccgt catggcgtcg agcccgtgga ggggctccag
     6001 ggcgcgcagc agcacggcgc tgcccgatcg ggccaccgcg ttcatgcagc ggtggatgcc
     6061 gtaggagacg tacacgtacg cgtggccggg cgggccgaac atcgtccggt tcccggacgt
     6121 ggggccgcgg aacgagtggc tggcggggtc gtcgggacgg tacgcctcgg cctccacgat
     6181 cctccccacg gtgcgcgccc ctccgagctc gctcacgacg accttcccca ggagggcccg
     6241 ggcgagcgcg agcgagggcc gctcgtagaa gtcgcgcggg agcgggcgcg tcaccggccc
     6301 ttccgcagga agcggcgcag cttcgtgatc ggccacgcgt tgatcacgtc gtccctcgtg
     6361 agccatccgc gctgcgccgt cccgatgccg aaccggaggt tgtcgaggtg cagggtcgag
     6421 tgggcgtcgg tatcgatggc gaacttgacg ccgtgtcgct tcgcccagag gacgtgctcg
     6481 tcgcgcagat cgaggcggtc ggggaaggag ctcacctcga gggcggtgcc ggtgcgcgcc
     6541 gcggccttga agacctcctc gaggtcgagc tcgatgggag gtcgcctgcc gatctgccgc
     6601 gccgtggggt ggccgatgat gttcacgcac gggtgctcca tcgcccggat catgcgcgtg
     6661 gtcatcttcg ccttgggcat cgtgaggtgg gagtggatcg aggccacgag cacgtcaaac
     6721 cccgagagga actcctcgtc ccagtccacc gatccgtccg gctggatgtt gagctcggag
     6781 ccgtggagga gggtcacctt ggaccgctcg tcgagctccc ggatgcgctc ccgttgggac
     6841 agcgccttct cccgcgtcat ccgctgcatg tagaggagct cggcgtgatc ggtgatcgcg
     6901 cagtaccggt agccgacccg ctcggccgcg gccaccatct cctcgagcgt ggccagaccg
     6961 tcggtgaggt ccgtgtgcgt gtggaggtcg cccttcacgt ccttccgctc gatcagcgtg
     7021 gggaggccgt ccccgagcgc cgcctcgatc tcgccccggt cctcgcggag gggcggaggg
     7081 atccagggca gaccgagccg ctcgtagacc tcctcctcgg tctcggccac gatcagcctc
     7141 ccgctctcgg ctcggaacag cccgtattcg gagagcttca gccccttgcg gacggccatc
     7201 tcccggacgt ggacgttgtg cgccttcgag ccggtgaagt actggagcgc ggccccccag
     7261 acggcgggct ccaccacgcg gaggtcgacc tggaggccct tcgtcgtgag caccgaggtc
     7321 ttcgtctcac ccttggccag cacctcacgg acgtaggggc ccgaggcgaa cgcgtccatc
     7381 accgggaccg ggtcctcgga ggccgccagg agatcgatgt ccccgatcgt ctccgccatc
     7441 cgccggaggg acccggcgaa ggtggcccgc cggacctggg gcacctcggt caggtggccg
     7501 agcagctcct cggccaggtc catggccacg ccgatctgca cgcgaccgcc ggagcgctgg
     7561 agcatctgga tgcctcgcag gaggttctcc tcggtcttgg gcccgaagcc cggcagctcg
     7621 cggatccgct gctcgtggag ggcgtcgagc agctcctcgg gggaggcgat ctggagctcc
     7681 tcgtagagct tcagcgcctt cttggggccg agggtgggga tgcccagcag ggcccgcacg
     7741 ccgtctggga tgtcggcccg gaggtcctcc agcgcctgga aggtgccggt ccggaggatc
     7801 tcctggatct tctcggccac cgaggcgccc acgttgggga tcgcccggca gccggccgcg
     7861 tcgagcgtcg agacctcggc gtggtagccg ccgaccgagc gcgcggcctt ctcgtaggcc
     7921 cgggacttga agctgtcgtc cccgaggatc gctagcaggt ccgagtactc ctgcaggtgg
     7981 gcggcgacct cgtcgttcga tctggccacg gctcaggcga tgccgagcag gcggagcagc
     8041 aaggccttct gggcgtggag gcggttctcg gcctggtccc agacgaccga gtgggggccg
     8101 tcgatcacct cgtcggtgat ctcctcgccc cggtgggccg ggaggcagtg caggacgaac
     8161 acgtcctccg cggccacggc caccttctcg ccgtcgagcc ggtacggggc gaacaccagc
     8221 acccgctcgg tggcctcggc ctcctgcccc atcgaggccc agacgtccgt gtagagcacg
     8281 tccaccccgg tcgccgccgc gatcgggtcg gccgtcacct cgacgctccc ccccgtctcc
     8341 gtcgcgatct cggcggcccg ctccacgatc tgggggatgg gctcgtagcc ggcgggcgtg
     8401 gccacccgca cgtgcatccc cgccttggcc ccgcccagca gcagggagtg ggcgacgttg
     8461 ttgccgtcac ccaggtacgc gagggtccgt ccggcgagct cccccttctg ctcgaggacg
     8521 gtgaggaggt cggcgaggca ctggcacggg tgctcgaggt ccgagagcgc gttcaccacc
     8581 gggaccgtcg aggtggacgc gagggcctcg aggcgctcct gcccgaacgt gcggaggacc
     8641 accacgtcga ggtaccggga gaggacccgg ccggtgtcct cgatcgtctc gccccggccc
     8701 agctgcagct cgctcgaaga gagggccagc gggtgcccgc ccatgagggc gataccgacc
     8761 tcgaacgaca ccctggtccg ggtggagggc ttctcgaaga tcatgcccac cgcccgcccg
     8821 gcgagcgccg tggcgtggtc ctcgggcgag gccttcacct tgcgggagag gtcgagcagc
     8881 ccgtgcaact cctcggccgt gaggtcgtcc atcgagagga agtggcgcgc cacgggccgg
     8941 agtctagcgc gggccccagc cgagggtcga acctcagtcg gccgcggcgt cggtggcgcg
     9001 agcccgcagc gcccgcagga ggtcgtctcg tccctcgagg aggacccgac ggatcgggcc
     9061 ggggaggtca tcccggccca ggacctcgtc ggtcatcgcc acggtctcct cggagacgat
     9121 cgccgacggg aaggcgccgc gggcgaacga gagggccagg gagaggtcga accgctccca
     9181 cgcgtcgtcc agggtgtcga agtagcggcg ggcgtagggg gcgaggacct cctcctgctc
     9241 ggggtgctgg aagccgccca ggatggcccg agccgacgcc agccgcggcg gctcggggcc
     9301 ggtcagcgcc tcccacgcct cggccttggc ctccgcggtg gggcgggccg cccgggcccc
     9361 ggaggcctca cgcgccccct cgtccgtggg gtcccgctcc tgctccgcgg cgatcaagcc
     9421 tgcgtccgct cgtcccgcgg aggccagcga gcgtacgagg tgccaccgca cgtccgtgtc
     9481 cacggccagc ccctcgatcc gctcctcccc gtcgagcaga gcgcgcacga gatccaggtg
     9541 ctcggggccg cgggccgcgg cggcgaacgt ccgggcccag atcagctgga ggtcgctgtc
     9601 cgccgccgcg gcccgcagcg cctgcagggc cgccccagcc agcgcgctcc cccccgcgtc
     9661 gcggttcgcc gggtcgccca gcagcgagac cgccgtggcc gcctgaccca ggatgcgctg
     9721 gacggccgag ggcatcgtct cggcgtggac gtggtccagc acggtcgcca ggaactcccg
     9781 ggcgggccag tccccgtccc gggtcatgtc ccagagcgcg cgccagcaga cggcacggct
     9841 gagcccgtcc ccgatccgcg agaggccctc ccggagggtc ccgagcgagc gtccgtcgaa
     9901 ccggaccttg gcgtacgtga ggtcctcgtc gttgagcagc acgagcggcg cgtccgagga
     9961 gagctccggc acctcggtcc gtgcgccggt gacgtcgagc tccgcgcggt gggcccggac
    10021 cagcttctcg ccctccggcg agaaagcccc gaccgcgagg cggtgcggcc ggaggggctc
    10081 cccctcctgg aggatcgcca cgccctgccc gacctcgggg gtgagcgcgt tgatcccgga
    10141 ggcgcggagc cagacgtcgg cccaggcccc gagatcccgt ttcgaggact cctcgtggac
    10201 ggccaggaag tccgcgagcg cggcgttccc gaacgcgtgg cgctcgaagt agacccgcat
    10261 cccctccagg aaccgctcca ggcccaccca ggccacgagc tggcgcatca cggcggcccc
    10321 cttcgcgtac gtgatgccgt cgaagttgag gaacaccgac tccacgtcgg ggacgtcggc
    10381 cacgatgggg tgcgtggtcg ggagctggtc ctgccgggag gcccacggct tctccgtgtg
    10441 ggcgaagctc gtccacggct ccgtgaagcg cgtggccctc tccatcgcca gcatcgaggc
    10501 gaacgtggcg aacgtctcgt tgagccacag gtcgtcccac cagcgcatcg tcaccaggtc
    10561 tccgaaccac atgtgcgcca tctcgtggag gatcgtttcg gcccgcccct cccgcctcgc
    10621 atcggtcacc ctggaccgga agatgtgcat ctcgttgaag gtgacgagcc cggggttctc
    10681 catcgcgccg gccgcgtact cggggacgaa gacctggtcg tacttcccga aggcgtaggg
    10741 cgtggcgaaa gcctcctcga agaagtcgaa cccctgcttc gtgatctcga ggatctcgtc
    10801 cgggtccaga tgctcggcga gggtcgcgcg gcagtagacc cccaggtcga tctcgccgcg
    10861 gcgatcgcgc gccacgtgga atggccccgc gacgacggcc gcgaggtagg tggacatcct
    10921 ggggctctcg gagaacgacc agcgccgggc gtcaccctcg gcctcgggct cggcatccgc
    10981 ggccatgttc gaggccacct cccaggaggc cggcgcggtc acgtcgatgc ggaacgtgcc
    11041 cttgacgtcg ggctggtcga agcacgggta gattcggtgg gcgccgaacg gctccgcgtc
    11101 ggagaagagg tagacggccc cgtccacggg gtcctcgaac ctggtcatgc cgtgctcccc
    11161 cgcccggtag gccatgtcgg ccaccacccg cacctcgttc cgctcggcga ggccgcccag
    11221 cgcgatccgg tgctcgccga ccgcgccgtc ggggagggcc tcaccgttca gctccgcggc
    11281 gtgcacctcc gccgccgtgc agtcgaggaa cgtcgaggcc ccgggctcgg cgacgaaacg
    11341 gatcacggtc tcgcaccgga actgctcggc gcccgtcagg tcgaggtgga tctggtaggc
    11401 gacgtcccgg acgagcgccg cgcggccggc ggcctcgtcc ctcgtgaggt tctccttcat
    11461 ttcaccccgc tctcagctcg gccccgacgt ccgacgccag ccgggcctcg atcgcggcca
    11521 cgagctcctc ggcctcctcg tcggtcagcg tgcggtcggc ggcccggagg tccacggcga
    11581 acgccaggga ccgcttcccc tcgggcagcg gctcgccatc gtacacgtcg aagagcagga
    11641 cgcggtcgag gagcgggccc gccgcgtcca cgagcgaggc ccgcacgtcc gcggccggca
    11701 ccgaccggtc cacgacgaac gcgaggtcgc ggcggaccgg ggggaagcgg gagacgggcc
    11761 ggagctcgac gagccccgcg cccgcggcca cgagaggggc ggcccggagt tcgaagacgg
    11821 ccacccgccc ggggacgtcg aaccgctccc ccaccgaggg gtgcagctcg ccgagctcgc
    11881 cgatcgcctc cccgtccagc agcacgcggg ccgaccggcc cggatggtac ggaggccccg
    11941 gcggatcgcc caggctccat ccgtccaccc cgagctcctc gaggagatgc tccagcgcgc
    12001 ccttcgcatc gaggacgtcc tgggggcggc gctccgccgg ccacgcctcg agggccgggc
    12061 cggtcagcag ggcggccacg gcgtcctcct cgaccacccc ctcctccagc cgcatcacgt
    12121 gccctcgttc gaagaggcgc accgccgccc ggcggtggcc gatcgagagc gcggccgccc
    12181 gcaggaggcc ggggaggagg ctcgatcgca ggaacccctg ctcctcggag acggggttgg
    12241 ccacccggac gcccgcggcc cgctcgtcct cgaacagctc gaggtcctcg gtcgaggcga
    12301 agctcaccga gtggatctgc atgaggcccg tgcggaccag cgcgtcctcg atgcggcgca
    12361 gggcccgctg ctcggggtcg agcccgcccg cctgggccac cccgggaagg gtcgagggca
    12421 gggcgtcgta gccccggacc ctccccacct cctcgatcag gtcggcctcg atctcgaggt
    12481 cgacccggcg gcccgggacc tcgacctcga cggcgccctc ccgctcctct ccctcgatcc
    12541 ccagccggcc catcacgtcc aggatgtcgc cggcggagac ctccatgccc aggagcatgc
    12601 tggccctggg cgggcgcacg gcgacccggc ggcgcggagg cgagcttccc acgtcgatct
    12661 cgcccgagag gacggtgccc ccggcccatg ccgcgacgag cccggccgcg tggagcgcgg
    12721 cgtcggccac cgcctcggga tccgctcctc gctcgaagcg gaccgacgcc tcggtccgca
    12781 gccccaggcg ccgggccgtc cggagcacgc cgcgcgggtc gaagtaggcg ctctccaaca
    12841 ggacgtcggt cgtggcgtct cccacctcgg tggcgtcgcc gcccatcacg ccggccacgg
    12901 cgacggcccg ctgggggtcg gcgatcatca ggtcctcggg cgtgaacgcc cgctccacac
    12961 cgtccagggt cacgagccgc tccccgtcgg tcgcccgccg cacgacgatc gcgccgccgc
    13021 tgatggtccg gaggtcgaag gggtgcatcg ggtgccccag ctccagcagg gcgtagttcg
    13081 tggcgtccac cacgttggag acggggcgca tcccacacgc cgtgaggcgg atctgggcgc
    13141 gcaggggcga tggggcgacg gtgacccccc ggatcacccg agccaggtac cgggggcacc
    13201 gctccgggtc gtcgacccgc accgaggcca ccgccgccgc cttctcgtcc ccctcgggga
    13261 cggacggggt gggcacccgc agctcgtcgc cggtgatggc ggccacctct cgggccacgc
    13321 cgagcaccga gaggagatca gggcggttgg ggaagacctc gatgtcgatc accgcatcgt
    13381 cgaggcccag gaacgccgcg acatcctccc ccgactcccc ctcgagcagg atcaggatgc
    13441 cgccgtggtc cgcggagatc ccgagctcct tcggggagca gagcatcccg tcggaggtct
    13501 cccctcggat cgtccgccgc tccagctgcc cgtcgaggcc cggcagggtc gcgccgggcg
    13561 gcgcgtaggg caccagatcg ccggggccca tgttccgcac accgaccacg acgcgccgct
    13621 cctccccgcc cgcgtcgacc gccgccaggg acaggcgatc cgccctcggg tggtcgctca
    13681 cctcgagcac ccgggccacg acgacgccgg tcagcccctc ccagggccgg aggacggact
    13741 ccacctcgac accgtgctcg gtgagggcga cggcgacctc ctcgggcgac agggacgtgg
    13801 ggacgagctc ccgcagccag gagtacggga ccttcatgac cagccccggg cgaactgctc
    13861 caggaaccgg acgtccccct cgtagaacag gcggatgtcg gggatgccgt gacgcagcat
    13921 cgctacccgc tccacaccga tcccccacgc gtagcccgtg tagcgctcgg ggtccacgcc
    13981 gccgttctcc aggaccctgg gatgcaccat cccggcgccc atgatctcca gccagccgtt
    14041 cccgcagatg cggcagcccg cgccgtcgca ggcgaagcac gagacgtcga cctgggcccc
    14101 cggctcgacg aaggggaagt agtcggggcg gaggcggacg cgctggcggg gggtgaacat
    14161 cgcccgcgcc cacgcctcga gcgtaccctt catgtcggcc agcgagagcc cctcgtccac
    14221 ggcgagcccc tcgacctgac ggaacacggg cgaatgggtg gcgtcgagcg tgtcgttccg
    14281 gtaggtgcgg cccgggacca ccacgtagag ggggggaggc tgggtctcca tcgccttgat
    14341 ctgcatcgga gaggtctggg tccggaggag caggccggac gcgcccggca cgtcgaggta
    14401 cagggtgtcc tggggcgtcc gcgccgggtg gtcgggcggg atgttgaggg cctggaagtt
    14461 gtaccagtcc gtctcgacct cgggcccctc catcacgcgg tagccgaggg ccacgaacgc
    14521 gtcgaccata gcccgctcgg tctgggtcag gacgtggagc gcgcccatcg gaaggcgcct
    14581 gccggggagg gtgacgtcga cggcgtccac ctccgccacc gcccgctccc gctcggccat
    14641 gagctcccgc tcccgctccg cgagggcctg gcggaaggac tcgaacacct cgttgatcgc
    14701 cttccccagc ctgggccggt cctccggcgc ggcctggccg agggacctct ggatcgcagg
    14761 gacgggagcc cgcctcccga gaacggcgac ctccgcgtcc cgcagctcct cgagcgaggc
    14821 ggcctcgccg atcgcggcga ggccgcgctc gcgctcccga tcgaggtcgg cgatcgtctc
    14881 cgcggggtcc atgtcgacgg ctagtctact gaccgtgacc atcccgacgc ggcaccggtg
    14941 ctagggatcc gctggtcgga cgccgacccc agcggcctgc tgatcttcac gatggaagtg
    15001 gagatccccc ggccccccga ggtggtgtgg ccctacctcg tggactggga ggggctcgac
    15061 cgctggatgg aggaggcccg ggccttccgg gtcctcagcg aggcgcggga aggcgtgggg
    15121 gtggaggcag aggccaccat ctcgatcctc gggatcacga cgagggacct ggtccgggtg
    15181 acgaggtggg agccgccggc cgtcctcgag atcgagcacc tggggtgggt gagcgggcgg
    15241 ggcctgatgg agctcggggt gacggccggc ggctcccacc tcttctggcg cgaggcgctc
    15301 cgtccgccgc tcgggcccct cggacggctc gggatgcgcc tgatgtcgcc ggtgatccac
    15361 cgcacgttcc gtcgggatct ggagacgctc cgcgcgctga tcgagcggga gacctaggcg
    15421 ggcaggcgca ggaggagccg gatccctccg tcggtctcgg cccccagcga cccccccagg
    15481 cgctccgcca cgagcctcac cgcgtagagg ctgacgcggc ccccgtgctc gggggacccg
    15541 aggacttccg tcggctcctc gtcctgccgg ttccccgctc gccacgcctc caccacggcg
    15601 tacgatccct cctcggcaac tctgagggac accggcccct cgctccccca ccacacggcg
    15661 ccgtccacga ggccgaagag cagggcgcgg atccggtcgg gatccgagcg ggcccgcgcc
    15721 tccccctcga cctcgacgtc ggggaagtcc tcgctggccg cgaagaggtc cgccagccac
    15781 ctggcggccg cgccgacgtc cgcgcgatcg ctcgtcgagg tgaggggcgc ccccgagagg
    15841 atgcgcaccg cgtcgaccac gaggcggacg agcgcgcccg tccggtggcc gtccagggcc
    15901 atgccctcga cgatctccct ccgttcctcg tcggagaacc cgtcccaccg cctgcccagg
    15961 gtcgtcacga accccgtgag cgcggtgagc ggcgagcgga ggtcgtggcc ggcggggccg
    16021 atcagcccgg cgatctcggc gagcccgccg ggcgcctctc gggctcggac ggcctcgaac
    16081 agcgtcacgg ccgccgaggc cgccaggttg agcgactcgg cccggccccg gatcggcacg
    16141 cggatcgtgg cgtcggccat cgcccggatc tccggctcca ggccgtgcgc ctcgttcccg
    16201 aacacgagcg ccgtcggcgg ggagaggtcg gcctcgtaga tcgtccgctc cccgtccgcg
    16261 gccgtcgcca ggacctggag ccctccgcga cgaagggcct ccagggcctc ggccacgtcc
    16321 acgtctcggg cgaccgggag gtggaagagc gagcccgccg acgcccgcac ggtcttgggg
    16381 ttgtagacgt ccaccgactc gcggccgaag accaccccgt ccgcccccgc ggcgtctgcc
    16441 gtgcggagga tcgtccccgc gttgcccggg tcccggaccg agctcagcac cgcgacgagc
    16501 gagggatcgg gctgcagctc ccccaggggg acgtccacgt aggtggccac cccgacgatg
    16561 ccctgcgggg tcacggtcga cgtgaggtgc tcgacgacct gctgggtgac ggcctcggtc
    16621 cgcacccccg actcccgggc ccggtccacc agagcgtcga cccggccgtg ctcgtccgtc
    16681 gtgtggaaga ggacctccac gcggccggcg tcgatcgcct cccgggtgac ctgggccccc
    16741 tccaccagga agcgcgcctc ccgatcccgg aaggcgcgct tcttcagctt ggccgccgcg
    16801 acgatgcgcg ggttcttggt ggaggtgatc acggccccgc ggccgcccgc ccctactccc
    16861 ccagcccccg gcgcgcgacc tcgacgagcc cggcgaagcc ctcggggtcg gtggcggcga
    16921 tgtcggccag gaccttccgg tcgaggtcga cgtccgcggc cttcaggccg gtcatgaacc
    16981 gggagtagga cagcccgtgc tgcctggagg ccgcgccgat gcgtgtgatc cacagccggc
    17041 ggaactcccc cttccgccgg cgacggtcac ggtaggcgta gacgcccgag tggagtagct
    17101 gctccttcgc cacccggtac ctgcgggagc gggcgccccg gtaacccttg gcctgggcca
    17161 ggatctcccg ccgcttcttc cgtccgtgga cgcctcgctt cacgcgcggc atcagcgcct
    17221 ccccaggagt cgcagaacgg tccgcctctc ggcggtcacc tcgttggtcc tggccagacg
    17281 ccgcttcctg gaggacgact tcttccccag gaggtggccg cggttcgctt tccggtgcag
    17341 aaccttgccg gatcgcgtca cgcggaagcg cttcgcggcg cctcggtggg tcttcgtctt
    17401 cgccatcctg tcctccctca ccccttcttc gtgggcgaga tcaccatcac catgttcctg
    17461 ccgtccagct tcggcatctg ctcgacctgc gccatgtcct ccaggtcccc caccagccgg
    17521 tcgaggatct tcctccccag ctcggtatgc gccatctcgc gtccccggaa catgatcgtc
    17581 accttcacct tgttgcccgc cgtgaggaag cgcacgacgt ggcccttctt cgtctcgtag
    17641 tcgtgggtgt cgatcttggg acggaacttg atctccttga cctccacccg cgcctgccgc
    17701 ttcctcgcct ctttctgccg gacgtcgcgc tcgtacttgt acttcccgta gtccatgatc
    17761 cgggcgaccg ggggcttcgc ctgcggcgcg acctccacga gatcgaggtc cagctcgtcg
    17821 gcctggcgca gcgcgtcccg tgtggagacg atgccgatct gcccgccatc gggtcctacc
    17881 aggcggacct gcggagcgcg gatccgctcg ttgatgcggg gttcaaccgg tacgccgaat
    17941 cacctccata cgaaacgcgc cggcgaacgg cggcggcgc
//
LOCUS       DASUTW010000229         5646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1495431, whole genome shotgun sequence.
ACCESSION   DASUTW010000229 DASUTW010000000
VERSION     DASUTW010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495431"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VF058_07870"
     CDS             complement(<1..350)
                     /locus_tag="VF058_07870"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6208256.1"
                     /translation="MTETYKLILAGQEADAASGDTFESTDPSTGKPFATVAKAGPEDV
                     QRAAEAARRAFDDGPWPRMKGSERARVLMDVASRIKEQGKELAETEAQDSGGTIRKGR
                     FSDVAMAISTFRVYA"
     gene            423..2060
                     /locus_tag="VF058_07875"
     CDS             423..2060
                     /locus_tag="VF058_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6208257.1"
                     /translation="MDPFVQEPPQPPDRFREDRPLRHALERLLPAEVFEEALPELAEM
                     AAIATGELQELANRAEANPPRHVPFDAWGRRVDLVEVDPAWTRLVEIGQERGVVALPY
                     EDRFGEHARVVQMGLLSLYLPVSATANCPLAMTDAAVRVLLNEDPELAERYVPRLTAR
                     SDAWASGQWMTETTGGSDVGRTETVASDRGDGTWALRGTKWFTSATTADVALALGRPE
                     GSEEGSRGLSLFLLELRDGDRWNGITVRRLKDKMGTRALPTAELDLDGTVAAPVGGIG
                     RGVAKIAAMLNVTRLHAAAGSLGAAGYGLSLARDYAGRREAFGRKLADLPVHRGWIAR
                     VAAEYEAMNALAFRAAELQGAVEQGSGVPELARVTIPLTKMALGRQGVWATSHLIESF
                     GGMGYLEDSGLPRLLRDAHVQSIWEGTTSVLALDVLRALGASGVAEAFGEDVAARTRA
                     ATASLLEEPAARVLAALETLRPMMAEPDEPQARRLAWGMARTYQAALLCEAADWALDV
                     HGDDRAATALKLFASEPLVGEEPPADPDALGRLAFGR"
     gene            2101..2439
                     /locus_tag="VF058_07880"
     CDS             2101..2439
                     /locus_tag="VF058_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6208258.1"
                     /translation="MPVIEECCPPLLLAPLKEDDAERLASALRVLADPARLRLVSIIG
                     AGPGGEACVCDLTEPMGLSQPTVSHHLKVLHEAGLLERERRGRWVYYRLVDGPLRSVA
                     EALSPAASTS"
     gene            complement(2370..2870)
                     /locus_tag="VF058_07885"
     CDS             complement(2370..2870)
                     /locus_tag="VF058_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6208259.1"
                     /translation="MAIEVRALAEEELEDALPLIAGYQVFYEAQPDDEHNRGFFRRFL
                     APSDEGLLLGAWENGRLAGFATIYWTYSSTLPGDIALMNDLFVAEDARGRGVGEALID
                     ACVEAARERGLPILEWYTATDNHRAQRLYDRYPGAEKTTWHAYDVEAAGESASATDRR
                     GPSTSR"
     gene            2939..3859
                     /gene="paaA"
                     /locus_tag="VF058_07890"
     CDS             2939..3859
                     /gene="paaA"
                     /locus_tag="VF058_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEX6208260.1"
                     /translation="MTSYEERTAEFKARIDAGEKIEAGDWMPDEYRRSALKFIEMHAN
                     SEVMGCLPEREWIPRAPSLRRKLSLTAKVQDEAGHGQILYRVAEDLGKSREQMFDDLV
                     NGRTKFHNVFHYPAETWGDVAVIGWLIDGAALVTQKALLDCSYAPYVRAMKRICAEES
                     LHLRHGEDLTLELVSGTDEQREMFQDAVNRWWKPIMHFFGPPSKPDKDVLLYWGIKTR
                     TNEDLRQEFFSTYVPKLWDLGISVPDSELRYDEEAGEWIWSDPDWDEFWQVVKGNGPM
                     TQTRLFWRKAIWDHHAWVRETFDRIPAAAV"
     gene            3870..4244
                     /locus_tag="VF058_07895"
     CDS             3870..4244
                     /locus_tag="VF058_07895"
                     /inference="COORDINATES: protein motif:HMM:NF018002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208261.1"
                     /translation="MWDVLQRRRAGADWEVVGGVKAPDADFAILLARESLFRRREGET
                     YAVRRRDTEDLIEVPDPTGIGGVIDREFRRMDSFAGVGGKHRRIHEQMRERGLVIDRP
                     RPPDRRQRRSAKQGDAEETARA"
     gene            4237..5106
                     /gene="paaC"
                     /locus_tag="VF058_07900"
     CDS             4237..5106
                     /gene="paaC"
                     /locus_tag="VF058_07900"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEX6208262.1"
                     /translation="MPEDDPRLILLLALADDELVIGHRHSEWTGWAPHLEEDLAFSSI
                     AQDEIAHARVLLELAEPLAGRGVDELALGRSPGEYRHAVICERPNGHWGYTVARQYLY
                     DTADDVRLAALEGSSDADLAAAVKTIRLEERYHVEHARIWFRRLAEGPVQAREHLSEG
                     LEQAVPEALALFEELPGEEALLADGVLPRSMSDLLSTWLVGLGADLESVSLDYVLSRH
                     KRVGELVPTSSGEAEEGSTLEVPGLERRGDRWVAVGGFAGAGGRRGKHTEDFDELWEE
                     MTMLYRKVPGATW"
     gene            5103..5624
                     /gene="paaD"
                     /locus_tag="VF058_07905"
     CDS             5103..5624
                     /gene="paaD"
                     /locus_tag="VF058_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017435996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEX6208263.1"
                     /translation="MTPIAAADLRDRVRRALERVMDPEIPTASIVDLGMVERVQVRDD
                     LVEVDLLPTFVGCPAKDVIGEDAEAAVREVSGDREVRMRFVFDPPWTTDRITEKGKAA
                     LREYGISPHWEGGPPDRGAVVVPLLAKPAGIPCPYCGSDRTVTESSFGPTPCRAAHFC
                     RACRNAFEGFKVK"
BASE COUNT          778 a         1972 c         2106 g          790 t
ORIGIN      
        1 gcgtacacgc ggaaggtcga gatcgccatc gccacgtccg agaagcggcc cttgcggatc
       61 gtgccgccgg agtcctgggc ctcggtctcc gcgagctcct tgccctgctc cttgatgcgg
      121 ctcgccacgt ccatcaggac ccgggcccgc tcggagccct tcatgcgcgg ccaggggcca
      181 tcgtcgaagg cccgccgcgc ggcctcggcc gcccgctgca cgtcctcggg gccggccttg
      241 gccaccgtgg cgaacggctt gccggtggag ggatcggtgc tctcgaaggt gtcgccggag
      301 gcggcgtcgg cctcctggcc cgcgaggatg agcttgtaag tctccgtcat gacgcggtcc
      361 tcctgggcgc ggatcgggtc gggccactat atcgcggggc cctcgtgcga tgatgcccgg
      421 gtatggaccc cttcgtccag gagccgccgc agccgcccga ccggttccga gaggaccgtc
      481 ccctgcgcca cgcgctggag cggctgctcc cggccgaggt gttcgaggag gcgctccccg
      541 agctcgccga gatggcggcg atcgccacgg gcgagctcca ggagctggcc aaccgcgccg
      601 aggccaaccc gccgcgtcac gtgcccttcg acgcctgggg acggcgggtg gacctcgtgg
      661 aggtggaccc cgcgtggacg cggctcgtgg agatcgggca ggagcgaggc gtcgtggcgc
      721 tcccgtacga ggatcggttc ggcgagcacg cccgggtggt gcagatgggt ctgctgtcgc
      781 tctacctgcc ggtgtcggcg accgcaaact gccccctggc gatgaccgac gctgccgtcc
      841 gggtgttgtt gaacgaggac ccggagctgg ccgagcggta cgtcccccgg ctgaccgccc
      901 gcagcgacgc ctgggcctcg ggccagtgga tgaccgagac gacgggcggc tcggacgtgg
      961 ggaggaccga gacggtggcc agcgatcggg gcgacgggac gtgggcgctg cgcggcacga
     1021 agtggttcac gtcggccacg accgccgacg tggccctggc gctgggccgg cccgagggat
     1081 ccgaggaggg gagccgcggc ctgtcgctct tcctcttgga gctgcgggac ggcgaccgct
     1141 ggaacgggat cacggtccgg cggctgaagg acaagatggg gacgagggcg ctcccgacgg
     1201 ccgagctcga cctcgacggg accgtggcgg cccctgtcgg agggatcggt cggggcgtgg
     1261 ccaagatcgc ggcgatgctc aacgtgacgc ggctgcacgc cgcggcgggg agcctcgggg
     1321 cggccggcta cgggctgtcg ctggcccggg actacgcggg gcgccgcgag gccttcgggc
     1381 ggaagctcgc cgacctcccg gtgcatcgag gatggatcgc gcgggtggcg gccgagtacg
     1441 aggcgatgaa cgcgctggcg ttccgggccg ccgagctgca gggggcggtg gagcaaggga
     1501 gcggggtccc cgagctggcg cgcgtgacga tccccctgac gaagatggcg ctgggccgcc
     1561 agggcgtctg ggccacgagc cacctgatcg agtcgttcgg agggatggga tacctggagg
     1621 actcgggtct cccacggctc cttcgggacg cccacgtcca gtcgatctgg gaggggacga
     1681 cctccgtgct ggccctcgac gtgctgcggg cgctgggggc ctccggcgtg gcggaggcgt
     1741 tcggggagga cgtggcggcg aggacccgcg cggcgaccgc ctcgctcctc gaggagcccg
     1801 ccgcgcgggt cctcgccgcc ctggagacgc tccgacccat gatggccgag cccgacgagc
     1861 cgcaggcccg gcgcctggcc tgggggatgg cccgcaccta ccaggccgcc ctgctgtgcg
     1921 aggccgccga ctgggcgctc gacgtccacg gggacgaccg ggccgccacc gcgctcaagc
     1981 tgttcgcgtc cgagccgctg gtgggggagg agccgccggc cgatcccgac gcgctcggcc
     2041 gcctggcgtt cgggcgttga cgcttctatc gaccaccgtc aatatggttc cgtgcgagag
     2101 atccccgtga tcgaagagtg ctgcccgccg ctgctcctgg cgcctctgaa ggaggacgac
     2161 gccgagcggc tggcatcggc cctccgcgtc ctggccgatc cggcccgcct ccgcctggtc
     2221 agcatcatcg gcgccgggcc cggcggcgaa gcgtgcgtct gcgacctcac cgagccgatg
     2281 gggctctccc agccgaccgt cagccaccac ctgaaggtgc tgcacgaggc gggcctgctg
     2341 gagcgcgagc gccgcggcag gtgggtctac taccggctcg tcgacggccc cctgcggtcg
     2401 gtggccgagg cgctctcgcc cgccgcctcg acgtcgtagg cgtgccaggt ggtcttctcc
     2461 gctcccgggt agcggtcgta gagccgctgg gcccggtggt tgtcggtggc cgtgtaccac
     2521 tcgaggatgg ggagcccccg ctcgcgggcc gcctcgacgc acgcgtcgat cagcgcctcg
     2581 ccgacccctc ggccccgcgc gtcctccgcc acgaacaggt cgttcatcaa cgcgatgtcg
     2641 cccggtagcg tcgaggagta ggtccagtag atcgtggcga agccggccag ccggccgttc
     2701 tcccaggcgc cgagcaggag cccctcgtcg ctgggcgcca ggaaccgacg gaagaaccct
     2761 cggttgtgct cgtcgtccgg ctgggcctcg tagaagacct gatacccggc gatcagcggc
     2821 agggcgtcct cgagctcctc ctcggccagc gcccgcacct cgatcgccat cgaggcatcc
     2881 tagcccggat ggtcggctcg ggcgccgggt agtatgaggg gcgaggagga cgcatcccat
     2941 gacgagctac gaggagcgca ccgccgagtt caaggcccgg atcgacgcgg gtgagaagat
     3001 cgaggccggc gactggatgc ccgacgagta ccgtcggtcg gccctgaagt tcatcgagat
     3061 gcacgccaac tccgaggtga tgggctgcct gcccgagcgc gagtggatcc cccgagcccc
     3121 gtctctccgc cgcaagctct cgctgaccgc gaaggtgcag gacgaggcgg gccacggaca
     3181 gatcctctac cgcgtggccg aggacctcgg gaagtcgcgg gagcagatgt tcgacgatct
     3241 cgtcaacggc cgcacgaagt tccacaacgt cttccactac ccggccgaga cctggggcga
     3301 cgtggccgtg atcggctggc tgatcgacgg cgccgcgctc gtcacgcaga aggccctcct
     3361 cgactgctcg tacgccccct acgtccgggc catgaagcgg atctgcgccg aggagtccct
     3421 ccatctgcgc cacggcgagg acctcaccct cgagctcgtc tccggcaccg acgagcagcg
     3481 cgagatgttc caggacgcgg tcaaccgctg gtggaagccg atcatgcact tcttcggacc
     3541 gccttcgaag ccggacaagg acgtcctcct ctactggggc atcaagaccc ggacgaacga
     3601 ggatctgcgc caggagttct tctccaccta cgtccccaag ctgtgggacc tcgggatcag
     3661 cgtccccgac tccgagcttc gctacgacga ggaggcgggg gagtggatct ggtccgaccc
     3721 cgactgggac gagttctggc aggtcgtcaa gggcaacggc ccgatgaccc agacgaggct
     3781 cttctggcgg aaggcgatct gggaccacca cgcgtgggtg cgggagacgt tcgacaggat
     3841 ccccgcggcc gcggtgtgac gcgatgatcg tgtgggacgt cctccagcgc cgtcgcgccg
     3901 gcgccgactg ggaggtcgtc ggcggggtga aggcgccgga cgccgacttc gccatcctgc
     3961 tggcccgcga gtccctcttc cggcgccgcg agggcgagac ctacgcggtc cgccgccgcg
     4021 acaccgagga cctgatcgag gtccccgacc cgaccgggat cggcggcgtg atcgaccggg
     4081 agttccgcag gatggactcg ttcgccgggg tcggcggcaa gcaccgcagg atccacgagc
     4141 agatgcgcga gcgcggactc gtgatcgacc gcccgcggcc tccggaccgc cgccagcgac
     4201 gctccgcgaa gcagggcgac gccgaggaga ccgcccgtgc ctgaggacga cccccgtctg
     4261 atcctcctgc tcgcgctggc cgacgacgag ctggtcatcg ggcaccgcca ctccgagtgg
     4321 acgggctggg ccccgcacct cgaggaggac ctcgccttca gctcgatcgc ccaggacgag
     4381 atcgcccacg ccagggtgtt gctcgagctg gccgagccgc tggccggccg tggggtggac
     4441 gagctcgcgc tgggccgctc gcccggcgag taccgccacg ccgtgatctg cgagcggccc
     4501 aacgggcact ggggctacac ggtggcccgc cagtacctgt acgacacggc cgacgacgtc
     4561 cggctcgccg cgctcgaggg gtcgtctgac gccgacctgg ccgccgccgt caagacgatc
     4621 cgcctcgagg agcgctacca cgtggagcac gcccggattt ggttccggcg cctggccgag
     4681 gggccggtcc aggcccggga gcatctgtcc gagggactgg agcaggccgt gcccgaggcc
     4741 ctggccctgt tcgaggagct gcccggcgag gaggccctcc tcgcggacgg tgtcctgccc
     4801 cgctccatga gcgatttgct gtccacctgg ctcgtggggc tcggggccga cctcgagtcg
     4861 gtctcgctcg actacgtgct gtcgcggcac aagcgagtgg gggagctcgt ccccacgtcc
     4921 tcgggcgagg ccgaggaggg gtcgacgctc gaggtgccgg ggctggagcg gcgcggcgat
     4981 cgctgggttg ccgtgggagg tttcgccgga gcaggggggc gccggggcaa gcacaccgag
     5041 gacttcgacg agctgtggga ggagatgacg atgctgtacc ggaaggtccc gggggcgacc
     5101 tggtgacgcc gatcgccgcc gccgacctcc gggatcgggt gcgccgcgcc ctggagcgcg
     5161 tgatggaccc ggagatcccc accgcctcga tcgtggatct ggggatggtc gagcgcgtcc
     5221 aggtgcgcga cgacctcgtc gaggtggacc tcctgcccac gttcgtcggc tgcccggcca
     5281 aggacgtgat cggggaggac gccgaggccg ccgtccgcga ggtctccggg gaccgcgagg
     5341 tccggatgcg gttcgtcttc gacccgccct ggacgaccga ccggatcacc gagaagggca
     5401 aggccgcgct ccgcgagtac ggcatctcgc cgcactggga gggcggcccc cccgaccggg
     5461 gcgccgtggt cgtccccctg ctggcgaagc cggcggggat cccgtgcccg tactgcggct
     5521 cggaccgcac ggtcaccgag tcgtcgttcg gacccacgcc gtgccgtgcg gcccacttct
     5581 gccgggcgtg ccgcaacgcc ttcgagggct tcaaggtgaa gtgagaggcg cccgccgcgg
     5641 agcggc
//
LOCUS       DASUTW010000230         4214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_459977, whole genome shotgun sequence.
ACCESSION   DASUTW010000230 DASUTW010000000
VERSION     DASUTW010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459977"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VF058_07910"
     CDS             <1..273
                     /locus_tag="VF058_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208264.1"
                     /translation="EERLIFPWGRELFRERLRHEVDTERPAFAEALGVAETIIELDGR
                     TLRLDVQISVAGDPERFHTAVSRRLTENGETIRERTWSEDVPRDHH"
     gene            complement(350..775)
                     /locus_tag="VF058_07915"
     CDS             complement(350..775)
                     /locus_tag="VF058_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX6208265.1"
                     /translation="MGKRASEKPRLLSGGNPQVPKGDGDEPVQTYIAAMPGWKRDVGR
                     YLDDLIERTVPDVRKAVRWNSPFYGIEGKGWFLSFHCFTRYVKVTWLNGSSLDPLPPG
                     ESKHEGVRYLDLHEDEDLDEQLIGSWIRQAAGLPGDETF"
     gene            complement(787..1428)
                     /locus_tag="VF058_07920"
     CDS             complement(787..1428)
                     /locus_tag="VF058_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208266.1"
                     /translation="MGRARRRASALAIGAGAAWGALYWLGRTWGSTREERRRYFPGDE
                     IVEPAKVVTNHAATLRATPEEIWPWLLQVGWHRGGWYTYRWVDRLLFPANRPAADSIL
                     SEYQDLKVGDRIPDGPPESGCFFEVEMLEPHKVMVLHSWTHLPPQVREARLDWTWTFH
                     LEPVGERETRFVFRVRGNLEPLWMRLLYHLAIVPADFVMGRSMCLGLKQRVER"
     gene            complement(1530..2192)
                     /locus_tag="VF058_07925"
     CDS             complement(1530..2192)
                     /locus_tag="VF058_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208267.1"
                     /translation="MNENESRTRWGAAVVALAPLVMAAGLFAHPFIAVVVDEAALAEA
                     VTSDPTRWALAHFVFAIGAVLLTLAFVVMRSYLRHAGEDRWSRWALPFAVLGSGLFAI
                     LPGLEFAPYAASEVGADIQATQEALVPFFFPLLVSGGVLFAIGAYGFARAIVASGVMS
                     RGMALVAATSLIVLAIARFVPLGVVQFYVHGAAALVALLPIALTMWGRPEAQMVGQPA
                     TG"
     gene            complement(2309..3337)
                     /locus_tag="VF058_07930"
     CDS             complement(2309..3337)
                     /locus_tag="VF058_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006127978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6208268.1"
                     /translation="MSDSPLTAPQAPGTTEAPLLEVRDLHVHFRGRVGLGAGLLGRKG
                     SQARAVDGVDLSIRRGEVLVLAGESGCGKTTLARTIMGLEKPTSGRILFEGKPVRPRD
                     LRAYRRQVQMVFQDPTGSLNPRQTIYETVAEGLRIHGIHDEEERRVADALSAAGLRPP
                     EALFLRYPHELSGGQRQRVVIAGALALEPSVIVADEPVSMLDASVKGEILKLLLGLRE
                     RLGLTVLLVTHDLGVAWTVADRVAVMYLGKIVELGPAERVLSEPAHPYTKALLEVAPE
                     HGGPRTLLTGEPPDPTRIPEGCRFHPRCPVVASGRAAAAGIEERCRGEIPLLREVGRD
                     HLAACHYA"
     gene            complement(3465..>4214)
                     /locus_tag="VF058_07935"
     CDS             complement(3465..>4214)
                     /locus_tag="VF058_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019992269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6208269.1"
                     /translation="DLLAMKPGRLRAVRWAEASIVFQGAMHSMNPVQRVGDQIAEAMV
                     LHGAAPDNGVRKEVGGLLERVGLPARRARDFPHEMSGGQRQRVMIAMALACSPSLVIA
                     DEPTTALDVMVQAQVLTLLRDLQRDLGLALLFITHDLSVLVELCDRIAVMYAGRIAEE
                     GPARAVFDDAKHPYAAALSGAFPRIGDPAYRGSPGGLGGDPPDPAALPSGCPFHPRCP
                     MRFEPCDGEEPGPFPAGDGRRAACYLLEPGR"
BASE COUNT          640 a         1537 c         1467 g          570 t
ORIGIN      
        1 gaggagcggc tgatcttccc gtggggtcgc gagcttttcc gtgagcgcct gcggcacgag
       61 gtggacacgg agcgtccggc cttcgcggaa gcgctcgggg tcgccgagac gatcatcgag
      121 ctggacggtc ggaccctccg cctggacgtg cagatctccg tggccggcga ccccgagcgc
      181 ttccacaccg ccgtctcgag acgcctcacc gagaacggcg agacgatccg cgagcgcact
      241 tggtccgaag acgtcccccg ggaccaccac tgatgggttc gtcccccagg cgatcaagac
      301 ctcacggcgg tcatgtccac cgcacaggat cgcagccgag cgcgccttct cagaaggtct
      361 cgtcgccggg caacccggcg gcctgacgga tccagctccc gatgagctgc tcgtcgaggt
      421 cttcatcctc gtggaggtcg aggtagcgca cgccctcatg cttggactcc ccggggggga
      481 gggggtccaa cgatgagccg ttgagccagg tcaccttcac gtacctggtg aagcagtgga
      541 agctgaggaa ccatcccttg ccctcgatgc cgtagaaggg cgagttccag cggaccgcct
      601 tccgcacgtc ggggacggtg cgctcgatga ggtcgtccag atagcggccg acatcgcgtt
      661 tccagccggg catcgcagcg atgtaggtct ggacgggctc gtcaccgtcg cccttcggaa
      721 cctgcgggtt gccgcccgac aggagtcgcg gcttctctga tgccctcttt cccatgcttc
      781 ttcatgctag cgctcaacgc gctgcttgag gccgaggcac atgctccggc ccatcacgaa
      841 gtcggccggg acgatcgcga ggtggtagag gagccgcatc cagagcggct cgaggtttcc
      901 gcgcacgcgg aacacgaacc tcgtctcgcg ctcgccgact ggctcgaggt ggaaggtcca
      961 cgtccagtcg agccgggcct cgcgcacctg cggcgggagg tgggtccagg agtgcagcac
     1021 catcaccttg tgcggctcga gcatctcgac ctcgaagaag cagcccgact cgggcggccc
     1081 gtcggggatg cggtcgccga ccttcaggtc ctgatactcg gagaggatcg agtccgcggc
     1141 cggccggttc gcggggaaga gaagtcggtc cacccagcgg tacgtgtacc agccgccccg
     1201 gtgccacccg acctggagga gccacggcca gatctcctcg ggcgtcgcgc ggagggtggc
     1261 ggcgtggttc gtgacgacct tggccggctc gacgatctca tcgcccggga agtagcgcct
     1321 ccgctcctcg cgcgtggacc cccacgtccg cccgagccag tacagggctc cccacgctgc
     1381 tcccgcaccg atcgccaggg cactcgccct ccgcctcgca cgacccatcc cgacacctcc
     1441 tggtcgagag tgtgaccgta ggcgtcggag ccccgatggg gccacttgtc ccccagaaga
     1501 ggggacgagt ctcccatcag gggaccggct caaccggtgg ccggctggcc gaccatctgg
     1561 gcctcgggcc ggccccacat cgtcagcgcg atcggcaaca gagccacgag ggccgctgcg
     1621 ccgtgcacgt agaactgcac cacgcccagg ggcacgaagc gggcgatcgc caggacgatg
     1681 agcgaggtcg cagccaccaa ggccatccct ctgctcatga ccccactggc cacgatggcc
     1741 cgcgcgaagc cgtaggcccc gatggcgaag agcactccgc cgctcaccaa cagtgggaag
     1801 aagaacggga cgagagcctc ctgggtcgcc tggatgtcgg cgccgacctc ggacgcggcg
     1861 tacggcgcga actccagccc cgggaggatg gcgaacagcc cgctcccgag cacggcgaac
     1921 ggcagggccc agcggctcca gcggtcctcg cccgcgtgac gcaggtagct ccgcatcacc
     1981 acgaaggcga gggtcaggag gacggctccg atggcgaaca cgaagtgcgc gagcgcccac
     2041 ctcgtcggat cggacgtgac ggcctcggcc agcgccgcct catccacgac gacggcgatg
     2101 aacgggtggg cgaacagccc ggccgccatc accagcgggg cgagggcgac gaccgccgct
     2161 ccccatcgcg tcctgctctc gttctcgttc atggtgtctc gcctccctgc gggcacacgc
     2221 atccagcgat gaccctcagt tccgacgcta ggggcgatga ggggcgcggg gaatcgggag
     2281 ttctccccat gcgagacggg cgccgcggct acgcgtagtg acaggcggcg aggtggtctc
     2341 gtccgacctc gcggaggagg gggatctcgc cgcggcagcg ctcctcgatc cctgcggccg
     2401 ctgctcgacc cgatgccacc accgggcacc tggggtggaa gcgacaccct tcggggatcc
     2461 gcgtcgggtc ggggggctcg cccgtcagaa gcgtcctggg ccctccgtgc tccggggcca
     2521 cctcgagcag cgccttcgtg tacgggtggg ccggctcgct gagcacccgc tcggccgggc
     2581 cgagctccac gatcttcccg aggtacatca ccgcgacgcg gtcggccacc gtccaggcca
     2641 cgccgaggtc gtgcgtcacg agcaggaccg tcagcccgag gcgctcgcgc aggccgagca
     2701 gcagcttcag gatctcgccc ttgaccgagg cgtcgagcat cgagacgggc tcgtccgcga
     2761 cgatcacgct cggctcgagc gcgagcgcgc ccgcgatcac gacgcgctgg cgctggcccc
     2821 cgctcagctc gtggggatag cgcaggaaca gcgcctccgg ggggcggagg cccgcggccg
     2881 acagcgcgtc ggcgacgcgg cgctcctcct cgtcgtggat cccgtggatg cggaggccct
     2941 cggccaccgt ctcgtagatc gtctggcgag ggttgagcga cccggtcggg tcctggaaga
     3001 ccatctggac ctgccggcgg taggcgcgga ggtcgcgggg gcggacgggc ttcccctcga
     3061 agaggatccg gcccgaggtg ggcttctcga gccccatgat cgtccgggcc agcgtggtct
     3121 tgccgcagcc ggactcgccg gccaggacga ggacctcgcc ccgccggatc gagagatcga
     3181 cgccgtccac cgctcgagcc tgggagccct tgcggccgag caagccagcg cccagcccaa
     3241 cgcgcccgcg gaagtggacg tggaggtcac ggacctcgag gagcggggcc tcggtcgtgc
     3301 cgggggcctg tggcgcggtg agcggcgagt cgctcatggt gcccggctcc caggggtggt
     3361 tcggatagcg gacaggtgac cgggatagcg gacacccatg cgaggcaccc ccctgggccc
     3421 cggtccatgg cggtgaacgc cggcgggcag gaggccgcgg ccactcatcg gcccggctcc
     3481 agcaggtagc aggccgcccg ccgcccgtcg ccggcgggga acggccccgg ctcctccccg
     3541 tcgcacggct cgaaccgcat gggacaccgc gggtggaacg ggcacccgct cggcagcgcg
     3601 gccggatcgg gcgggtcgcc ccccagcccg cccggcgacc cccggtacgc gggatccccg
     3661 atccgcggga acgcccccga cagcgccgcc gcgtacgggt gcttcgcgtc gtcgaagacg
     3721 gctcgggccg gcccctcctc cgcgatccgt cccgcataca tcaccgcgat ccggtcgcag
     3781 agctccacga gcaccgagag gtcgtgcgtg atgaacagca gggcaagccc caggtcccgc
     3841 tggaggtccc gcagcagcgt cagcacctgg gcctggacca tcacgtcgag cgcggtcgtg
     3901 ggctcgtctg cgatcaccag cgagggcgag cacgcgagcg ccatcgcgat catcacccgc
     3961 tggcgctgcc cgccgctcat ctcatgtggg aagtcgcggg cccgccgcgc gggcagcccg
     4021 acgcgctcca gcagcccgcc cacctccttc cgcactccgt tgtccggcgc cgccccgtgc
     4081 aggaccatcg cctccgcgat ctggtcgccc acccgctgga ccgggttcat cgagtgcatc
     4141 gcgccctgga agacgatcga ggcctcggcc caccgcaccg cgcgcaggcg ccccggcttc
     4201 atcgccagca ggtc
//
LOCUS       DASUTW010000231         2375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_720263, whole genome shotgun sequence.
ACCESSION   DASUTW010000231 DASUTW010000000
VERSION     DASUTW010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720263"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VF058_07940"
     CDS             complement(<1..375)
                     /locus_tag="VF058_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX6208270.1"
                     /translation="MATTETGYRAPEVVKLVGVTYRQLDYWARTELVKPSVRDANGSG
                     TQRLYGFQDLVLLRSIKNLLETGVTLQKIRKAVDFLRGHLGEAPQEMTLLSDGRNIYA
                     AKSRDEIIDLMQQGQGTMFTLQV"
     gene            complement(447..1955)
                     /locus_tag="VF058_07945"
     CDS             complement(447..1955)
                     /locus_tag="VF058_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HEX6208271.1"
                     /translation="MADDQAAFEALQAKLVPLWSSIESFNPDPQTIVVVPALPPGIFS
                     GSRVQAYEERYLFLLLLLRQPNARMIYVSSMPIDPLILDYYLGLLPGVIPSHARERLF
                     AVAPGDGSDASLSDKLLARPRLLGRIRSLIPDPDRAHLVPFFATERERRLALALGIPM
                     YAADPRFARFGTKSGCRRLFSEEGVPHPAGREGVRDLDDVAAAIEELRRQRPGMRAAI
                     VKLDEGVSGMGNATVDLEGLPDPDGASAGDALAERVRTMSTEVGEPVEAYAERLAGEG
                     GIVEERVEGADVRSPSVQMRVSPLGKLEVLSTHDQLLGGPSGQTYLGARFPADPEYAP
                     MISREGRKVGERLAAEGVIGRFAVDFVVTRDVGAWQPYAIEVNLRKGGTTHPFLTMQF
                     LTDGSYDETTGAFTAPSGRAKSFVASDHVESDAYRGLTPSDLFDLAVRRGLHFDPARQ
                     CGVVFHMMTALPEHGQTGLTAVGDSSQEADDLYRRTVEVLDEETRGAYAPDA"
     gene            complement(1995..>2375)
                     /locus_tag="VF058_07950"
     CDS             complement(1995..>2375)
                     /locus_tag="VF058_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017591351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX6208272.1"
                     /translation="WIGAAEATAIALAMQGVTPMRPMTHDLFKNVLDELHVALEKIEI
                     TELQEGTFYAMLHLQRNGSSHEVSSRPSDAIALAVRTGVPIFAAEDVLEAAAVEIHDD
                     DAEEEVERFKQFLEDVTPEDFAEE"
BASE COUNT          341 a          848 c          829 g          357 t
ORIGIN      
        1 cacctggagg gtgaacatcg tcccctgacc ctgctgcatc aggtcgatga tctcgtccct
       61 cgacttggcg gcgtagatgt tccggccgtc ggagaggagg gtcatctcct ggggcgcctc
      121 gccgaggtgc ccgcggagga aatccaccgc cttccggatc ttctggaggg tcacaccggt
      181 ctccagcagg ttcttgatcg agcggagcag caccaggtcc tggaagccgt agagcctttg
      241 ggtccccgac ccgttggcgt cccgcacgct gggcttcacc agctcggtgc gcgcccagta
      301 gtccagctgc cggtaggtga cgcccacgag cttcaccacc tcgggggcgc ggtatcccgt
      361 ctcggtcgtc gccatggcca cctctcccag tcacattacc gtaattacga gagtgtggcc
      421 gagcctagcg tcgggacccg ggggggtcaa gcatccggcg cgtaggcgcc ccgcgtctcc
      481 tcgtccagga cctccacggt ccggcggtac aggtcgtcgg cctcctgaga gctgtcgccc
      541 accgccgtca ggcccgtctg cccgtgctcg ggcagggccg tcatcatgtg gaagacgacg
      601 ccgcactgcc gcgcggggtc gaagtggagg ccgcggcgga ccgccagatc gaagaggtcc
      661 gagggcgtga gcccgcgata ggcatccgac tcgacgtggt cgctggccac gaaggacttc
      721 gccctcccgc tgggggccgt gaaggccccg gtcgtctcgt cgtacgaccc gtcggtgagg
      781 aactgcatcg tgaggaacgg atgggtggtc ccgcccttcc ggaggttcac ctcgatcgcg
      841 tagggctgcc acgcgccgac gtcccgcgtc acgacgaagt ccacggcgaa gcgcccgatc
      901 accccctccg ccgccagccg ctcccccacc ttgcggccct cccgagagat catcggagcg
      961 tactccgggt cggccgggaa gcgggcgccg aggtaggtct gcccgctcgg gcctcccagc
     1021 agctggtcgt gggtggagag gacctcgagc ttccccaacg gggagacgcg catctggacg
     1081 ctcgggctcc ggacgtcggc gccctccacg cgctcctcga cgatcccgcc ctcccccgcc
     1141 aggcgctcgg cgtacgcctc gaccggctct ccgacctccg tcgacatcgt ccgcacgcgc
     1201 tcggccaggg cgtccccggc cgacgcgccg tccggatcgg gcagcccctc caggtcgacg
     1261 gtggcgttgc ccatcccgga gacgccctcg tcgagcttca cgatcgcggc ccgcatgccc
     1321 ggccgctgcc gacgcagctc ctcgatcgcg gcggccacgt cgtcgaggtc gcggacgccc
     1381 tccctgcccg cagggtgtgg gacgccctcc tccgagaaga gccgccggca cccgctcttc
     1441 gtcccgaacc gcgcgaagcg agggtcggcc gcgtacatcg ggatccccag ggccagggcc
     1501 aggcgccgct cccgctccgt ggcgaagaac gggacgaggt gggcccggtc gggatcgggg
     1561 atcaggctcc ggatccggcc caggagacgg ggccgcgcca gcagcttgtc gctcagcgag
     1621 gcgtcggacc cgtcgccggg ggccacggcg aacagccgct ccctggcgtg gctggggatc
     1681 accccgggca gcaggccgag gtagtagtcg aggatcagag ggtcgatcgg catcgagctg
     1741 acgtagatca tccgcgcgtt cggctggcgc aggagcagga ggaggaacag atagcgctcc
     1801 tcgtaggcct ggacccgcga gcccgagaag atccccggtg gcagcgcggg gacgacgacg
     1861 atcgtctggg gatcggggtt gaaggactcg atcgagctcc agagcgggac gagcttggcc
     1921 tgaagagcct cgaacgccgc ctggtcgtcg gccacggccg atgataggcc gcccgccgtc
     1981 gcccgggcca gcccctactc ctcggcgaag tcctccgggg tgacgtcctc gaggaactgc
     2041 ttgaagcgct cgacctcctc ctcggcgtcg tcgtcgtgga tctccacggc ggccgcctcg
     2101 agcacgtcct cggcggcgaa gatcgggacg ccggtgcgaa cggccaaggc gatcgcgtcg
     2161 ctggggcggg aggagacctc gtggctcgag ccgttgcgct ggaggtgcag catcgcgtag
     2221 aacgtgccct cctggagctc ggtgatctcg atcttctcca gggccacgtg cagctcgtcc
     2281 aggacgttct tgaagaggtc gtgggtcatg ggccgcatcg gcgtgacgcc ctgcatcgcc
     2341 agcgcgatcg cggtggcctc cgcggcgccg atcca
//
LOCUS       DASUTW010000232         3541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1755756, whole genome shotgun sequence.
ACCESSION   DASUTW010000232 DASUTW010000000
VERSION     DASUTW010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755756"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VF058_07955"
     CDS             <1..849
                     /locus_tag="VF058_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX6208273.1"
                     /translation="HGTNDMLNFLAARRGYCEQFAGTMAVMLRLLGYPARVAIGFTAG
                     DADEDGVFRVTTEQAHAWVEMYFPLYGWLPFEPTPTRDNPTASHLTAPLPELPGTGQG
                     PDAATQQGGRFQAQDPRLDHVERRFGIGPAARLQLEEEEPTSLWPRIVLGLLLVLLAI
                     GGVPTWKWISRRRRLRRASSPREAVLAAFDVLEERAADVGLERRPEETLLEYEARLRG
                     TITLRDGDLERMTGLVTRAAYGSDDVDAAEARLAADALKILTADLRRHAGRGRALVGA
                     IRPARD"
     gene            complement(846..1334)
                     /locus_tag="VF058_07960"
     CDS             complement(846..1334)
                     /locus_tag="VF058_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX6208274.1"
                     /translation="MEGFTSHQASRLTGCTRRQLRYWDEIGLVNPSVQSTGGRPGVPR
                     LYSFRDLIALKVVKSLLDGGMSLQRVRRAYGFLRERAGMDVHLSEVKLITDGKSIFKV
                     ARREGEILDALRNGQMAFFVALDSIVRSIDAEVEEFRQDRRQFERALREATRQVQVEA
                     AT"
     gene            complement(1451..1828)
                     /locus_tag="VF058_07965"
     CDS             complement(1451..1828)
                     /locus_tag="VF058_07965"
                     /inference="COORDINATES: protein motif:HMM:NF022684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HEX6208275.1"
                     /translation="MPLSDHEQRILEEIEKRLREEDPRLAETVAKATVHAHIVRRVRL
                     GIVAFVAGFVMLMLFMVSIWVALAGFGVMLASALFVYHTIRRMAHDQMEAARREGGPS
                     VIGSLARLVDRFRGRSEPPADQR"
     gene            complement(1887..3068)
                     /gene="dinB"
                     /locus_tag="VF058_07970"
     CDS             complement(1887..3068)
                     /gene="dinB"
                     /locus_tag="VF058_07970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018500069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX6208276.1"
                     /translation="MIRPPEPILHVDMDAFYASVEVLKDPALKGKPVVVGGSGSRGVV
                     MSASYESRRFGVRSAMPAARARRLCPEAVFVRPDFSSYRAYATRLREIFLSFTPLVEP
                     LSLDEAFLDVGGASRLFGEPVEIAEKVRARVLEETELVCSVGVAPNKFLAKMASTQAK
                     PNGLLVVPAGGVEAFLHPLPVDALWGVGEKTDELLRRLGVRTIGELAATSPRVLERVL
                     GEQQGRHLAALARGEDDRSVIPYEAPKQVSHEETFDRDLDAEDDVRRELLRLSFRVAA
                     RLRKEGYRARTVTLKARLASFTTLTRSRTLPDPTDVGNDLYRIVAELFGALPSRGRLR
                     IRLLGVAAAGLRPSGVDQLALVRAGRWDDAERALDRIERRFGEGAAFPASLLDRGRSR
                     G"
     gene            complement(3065..>3541)
                     /locus_tag="VF058_07975"
     CDS             complement(3065..>3541)
                     /locus_tag="VF058_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208277.1"
                     /translation="DLGQRSLGFLWSMQGVGALLASILLIGLRRATGAEMVVIAFGVL
                     IAGAGLLVYAATGVFGVAIPGAILFGFGFPLFYSTGQALIQRISSAPGKVSAVFVVIS
                     EVGPLVAALGIGVLGRVDVQVWLVGAAGGLLVFGGMGLWASRRPALLVEEAPASAS"
BASE COUNT          551 a         1328 c         1232 g          430 t
ORIGIN      
        1 cacggcacga acgacatgct caacttcctc gcggcgcgtc gcgggtactg cgagcagttc
       61 gcggggacga tggcggtgat gctgcgcctc ctcggctacc cggcgcgggt ggcgatcggg
      121 ttcaccgccg gggacgccga cgaggacggc gtcttccggg tgacgaccga gcaggctcac
      181 gcgtgggtcg agatgtactt cccgctctac gggtggctcc cgttcgagcc cacgccgacg
      241 cgcgacaacc ccaccgcctc ccacctcaca gcgcccctgc cggagctccc cggcacgggg
      301 caggggccgg acgcggccac gcagcagggc ggtcggttcc aggcccagga cccgcgcctg
      361 gaccacgtgg agcggcggtt cgggatcggc ccggccgcgc gcctccagct cgaggaggag
      421 gagcccacat cgctctggcc ccggatcgtc ctgggcctcc tgctcgtgct cctggccatc
      481 ggcggcgtgc cgacctggaa gtggatctca cgccggcgcc gcctgcggcg cgcctcgtct
      541 ccgagggagg cggtcctggc ggccttcgac gtgctggagg agcgggccgc ggacgtgggg
      601 ctcgagcgcc gtcccgagga gaccctgctg gagtacgagg cgcgcctccg cggcacgatc
      661 acgctgcgag acggcgacct ggagcggatg acggggctcg tgacccgcgc ggcctacggg
      721 agcgacgacg tggacgccgc ggaggcgcgt ctcgccgccg acgccctcaa gatcctcacc
      781 gccgatctgc ggcggcacgc ggggcggggc cgcgcgctcg tgggggcgat ccgccccgcc
      841 cgcgactagg tagccgcctc gacctggacc tgcctcgtgg cctcccgcag cgcccgctcg
      901 aactggcgcc ggtcctgccg gaactcctcg acctcggcgt cgatcgatcg cacgatcgaa
      961 tccagcgcca cgaagaaggc catctgcccg ttcctgagcg cgtcgaggat ctcaccctcc
     1021 cggcgggcca ccttgaagat gctcttgccg tcggtgatga gcttgacctc ggacaggtgc
     1081 acgtccatcc ccgcgcgctc gcggaggaac ccgtaggcgc gacggacgcg ctgcagcgac
     1141 atccccccgt cgagcagcga cttcacgacc ttcagcgcga tgaggtcgcg gaacgagtag
     1201 aggcgcggga cgccgggccg tccgccggtg ctctgcaccg acgggttcac gagcccgatc
     1261 tcgtcccagt agcgaagctg tctgcgggtg cagcccgtca ggcgcgatgc ctggtgcgac
     1321 gtgaagccct ccatcgaacc tccgatcgaa ctcgcccggg cgggagcttt ccgaccgcat
     1381 ggcccccggg ggaagtggta gacgtagttg tacggcaacg gggacccggt gcgcaagcaa
     1441 gaaaagggat tcagcgctgg tcggcgggag gctccgagcg gccgcggaag cggtcgacga
     1501 ggcgggccag ggacccgatc accgacggcc ctccctcccg acgggccgcc tccatctggt
     1561 cgtgggccat ccgccggatc gtgtggtaga cgaagagcgc cgacgcgagc atcacgccga
     1621 aacccgccag cgccacccag atcgacacca tgaacagcat cagcatcacg aagcccgcca
     1681 cgaaggcgac gatgccgagg cggacccgac gcacgatgtg ggcgtggacg gtggccttgg
     1741 ccacggtttc ggccaggcgg gggtcctcct cccggaggcg cttctcgatc tcctcgagga
     1801 tgcgctgctc gtgatccgac aggggcatgc cggccgctcc tttcctcgcg cccaattata
     1861 gggagtaccc caccttgggg gtcccctcaa ccacgggacc ggccccggtc gaggagcgag
     1921 gccgggaacg cggcgccctc gccgaaccgc cgctcgatcc gatcgagcgc ccgctccgcg
     1981 tcgtcccagc ggccggcccg gaccagcgcg agctggtcca cgcccgatgg gcgcaggccc
     2041 gccgccgcaa cgcccagcag gcggatccgc aggcgcccgc gtgaggggag cgccccgaag
     2101 agctccgcta cgatccggta gaggtcgttg cctacgtcgg tcgggtcggg cagcgtcctc
     2161 gaccgggtga gggtcgtgaa gctggcgagc cgggccttga gggtcacggt cctcgcgcgg
     2221 tacccctcct tccgcaggcg cgccgccacc cggaacgata accgcagcag ctcccgacgg
     2281 acgtcgtcct cggcgtcgag gtcccggtcg aaggtctcct cgtggctcac ctgcttcggg
     2341 gcctcgtacg ggatcaccga acggtcgtcc tcgccgcggg ccagcgcggc gaggtgccgg
     2401 ccctgctgct cccccagcac ccgctccagg acgcgcggcg aggtcgcggc cagctctccg
     2461 atcgtccgca cccccagccg ccggagcagc tcatcggtct tctctcccac gccccacagc
     2521 gcgtccacgg ggagcggatg aaggaacgcc tcgacgccgc ccgcgggcac cacgagcagg
     2581 ccgttgggct tggcctgggt ggaggccatc ttcgccagga acttgttcgg cgccacgccc
     2641 acggagcaga cgagctcggt ctcctcgagg acgcgcgccc ggaccttctc cgcgatctcg
     2701 accggctccc cgaacagccg ggaggcgccc cccacgtcga ggaacgcctc gtccagggag
     2761 aggggctcca cgagcggcgt gaacgagagg aagatctcgc ggaggcgcgt ggcgtaggcc
     2821 cggtaggacg agaagtcggg gcggacgaag accgcctccg ggcagaggcg gcgggcccgg
     2881 gccgccggca tcgccgaccg caccccgaac cgccgcgact cgtaggaggc cgacatcacc
     2941 acgccgcgcg acccggagcc tcccacgacg accggcttcc ccttcagcgc ggggtccttc
     3001 aggacctcga ccgacgcgta gaacgcgtcc atgtccacgt ggaggatggg ctcgggagga
     3061 cggatcacga cgcgctcgcg ggggcctcct ccacaaggag ggcgggacgg cgcgaggccc
     3121 acagacccat cccgccgaac acgagcaggc cccccgcggc gccgacgagc cacacctgca
     3181 cgtccacccg ccccaggacg ccgatgccga gcgccgccac gaggggcccc acctcgctga
     3241 tcacgacgaa gacggcgctg accttccccg gcgcgctcga gatccgctgg atcagggcct
     3301 ggcccgtgga gtagaagagg gggaacccga acccgaacag gatcgcgccc gggatcgcca
     3361 ccccgaacac gccggtcgcc gcgtacacaa ggagccccgc ccccgcgatc aggacgccga
     3421 acgcgatcac caccatctcc gcgccggtcg cccgccgaag cccgatcagc aggatcgagg
     3481 cgagcagcgc gccgacgccc tgcatcgacc acaggaagcc cagggaacgc tgcccgagat
     3541 c
//
LOCUS       DASUTW010000233         4643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_720484, whole genome shotgun sequence.
ACCESSION   DASUTW010000233 DASUTW010000000
VERSION     DASUTW010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720484"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VF058_07980"
     CDS             <1..568
                     /locus_tag="VF058_07980"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX6208278.1"
                     /translation="IVFRLIERMGGGMSDEDAKSLYAGVVTDTGRFQYEATTPETLRV
                     GAALRERDFDHARLAQALYEDGSFAALGVMGKALERAQLDGDLRLVWTWITRKDLEEA
                     GVQIQETDDMIDAIRVAREADVAAVLKEQREGGFKVSLRSRGDTNVAAVAEAFGGGGH
                     RLAAGYSWEGGLDEAVAALRKALRQAAT"
     gene            578..1423
                     /gene="truB"
                     /locus_tag="VF058_07985"
     CDS             578..1423
                     /gene="truB"
                     /locus_tag="VF058_07985"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018504594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX6208279.1"
                     /translation="MSGPEGIVLVDKPKGITSHDAVDQVRRALGTRKVGHAGTLDPMA
                     TGLLVMGVGRATRLLRFLSGLEKEYEGTGRLGVETDTLDAEGEVVREAPVAVDERALR
                     DAMASLTGEIDQRPPAYSAVRVGGERLYRAARRGEAVEAPARRVRVETFELARYEAPE
                     LDFRVVCSSGTYVRSLVADLGARLGCGAHLTRLVRTRIGPYRIAQAAPPERVEAPLPI
                     ERAVVHLPRVELDEEEARAARHGRALGPSGHDGPHAVFDADGRLIGVWQDEGPRARPL
                     VVLAG"
     gene            1449..2372
                     /locus_tag="VF058_07990"
     CDS             1449..2372
                     /locus_tag="VF058_07990"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX6208280.1"
                     /translation="MEVITELEAVRPDGPCAVTIGFFDGVHRGHQTVIRRLVAAAEER
                     DLRPVAVTFDRHPREVLTPGDVPGLLTTTDRKAHLIEELGVDLLVVMPFDAEVASWPP
                     ERFVDDVLVERLGVAHATVGANFTFGHRAAGTVEVLTDLGRARGFSVEGVGLLEVEGR
                     RISSTSIREALAAGDLEWPRVALGRRHVIDGVVVPGAGRGDDLGWPTANLQTDPRALL
                     PARGVYAGRGLLEEGAWPAAINVGINPTFGEEPLHVEAFLLGFEGDIRGRDLAIELWE
                     RLRDEERFESSEALSRQIERDVERTRAVVSE"
     gene            2416..2682
                     /gene="rpsO"
                     /locus_tag="VF058_07995"
     CDS             2416..2682
                     /gene="rpsO"
                     /locus_tag="VF058_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX6208281.1"
                     /translation="MITKEDKQGLIAEYRQGEGDTGSPEVQVALLTKRIEELNEHLKT
                     HRKDHHSRRGLLLMVGKRRRLLEYLKRDDIERYRALIGRLGLRR"
     gene            2733..>4643
                     /locus_tag="VF058_08000"
     CDS             2733..>4643
                     /locus_tag="VF058_08000"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX6208282.1"
                     /translation="MEPITVSGKVGGTDLSFETGRLAKLAHGAAVVTLGETQVLVTCA
                     WGAPREGIDFFPLTVDVEERMYAAGKIPGGFFRREGRPSEQAILTCRLTDRPLRPTFR
                     EGFRQEVHVVSTILSADQANPYDIITINGASAAVMLAGLPFDGPIGAVRVAMISGSWV
                     ANPTYQDLDLATFDMVVAGRRNDAGGTDILMVEAEAPEEALSHMSEGQPAPTEEVVAE
                     GLEEAKRAIGEAIDLQEELVRRAGVEPKEFPVTVDYEDEVMEAVQEVAGSGLSDALTI
                     ADKTERNERLDAIKEDLGTSLTERWGEETYEERARQVKAAFKALQKEIMRKRVIEEGI
                     RIDGRKPDEIRPISCAVDLVPRAHGSGLFERGETQVLNVATLGMLRMTQMIDTLDPEE
                     EKRYMHHYNFPPFSTGEPGFMRGPKRREIGHGALAERALASVIPSEEEFPYALRLVSD
                     VLASNGSTSMASACASTLSLMDAGVPIKAPVAGIAMGLIVEGDRFVTLTDILGAEDAL
                     GDMDFKVAGTKDFITALQLDTKVTGLPAEVLSGALKQAREARLFILDRMLETIPEPRS
                     ALSAKAPRVEAIQIPVDKIGEVIGPKGKRINEIIAETGADVDIHDDGTVFIGSKEGEG
                     AERAKEMINAIVN"
BASE COUNT          706 a         1567 c         1768 g          602 t
ORIGIN      
        1 gatcgtcttc cggctgatcg agcgcatggg aggcgggatg tcggacgagg acgcgaagtc
       61 gctgtacgcg ggcgtcgtga ccgacaccgg gcggttccag tacgaggcga ccacgcccga
      121 gacgctccgg gtgggggcgg cgctccggga gcgggacttc gaccacgccc gcctggccca
      181 ggccctctac gaggacggct cgttcgccgc gctgggcgtg atggggaagg ctctggagcg
      241 cgcccagctg gacggagatc tccgcctggt ctggacctgg atcacgcgga aggacctcga
      301 ggaggccggt gtccagatcc aggagaccga cgacatgatc gacgcgatcc gagtggcccg
      361 ggaggcggac gtggccgcgg tgctgaagga gcagcgcgag ggaggcttca aggtctcgct
      421 ccgctcccgc ggagacacga acgtggcggc ggtggccgag gccttcggcg ggggagggca
      481 ccgcctggcg gccggctact catgggaggg cggcctggac gaggccgtcg ccgcgctgcg
      541 gaaggccctg cgccaggcgg cgacctaggc agccgcggtg agcgggcccg aggggatcgt
      601 cctcgtcgac aagccgaagg ggatcacgtc tcacgacgcc gtggatcagg tccggcgggc
      661 gctgggcacg cggaaggtgg gacacgccgg gaccctcgat cccatggcga cggggctcct
      721 cgtgatgggc gtggggaggg ccacgcgcct cctgcggttc ctgtcgggcc tggagaagga
      781 gtacgagggg accggccgtc tgggcgtgga gaccgacacg ttggacgccg agggcgaggt
      841 cgtccgggag gccccggtcg cggtggacga gagggccctt cgcgacgcga tggcctcgct
      901 cacgggcgag atcgaccagc ggccgcccgc gtactccgcc gtgcgggtgg gcggggagcg
      961 gctctacagg gcggctcgca ggggggaggc cgtggaggcc ccggcccggc gggtccgcgt
     1021 ggagacattc gagctcgcgc ggtacgaggc gcccgaactg gacttccgcg tggtgtgctc
     1081 aagcggcacc tacgtgcgct cgctcgtcgc ggatctgggc gcgcgcctgg ggtgcggcgc
     1141 ccacctgacc aggctcgtcc gcacccggat cggtccctat cggatcgccc aggccgcgcc
     1201 cccggagcgg gtggaggcgc ccctcccgat cgagcgcgcc gtggttcacc tgccgcgggt
     1261 ggagctcgac gaggaggagg cccgagcggc gcggcacggg cgggccctcg ggccctccgg
     1321 ccacgacggg ccccacgccg tcttcgacgc cgacgggcgg ctgatcgggg tctggcagga
     1381 cgagggcccg agggcccgcc cgctcgtggt cctggccggc tgaggcctgc gctaccctca
     1441 cttccgcgat ggaggtgatc acggagcttg aggccgtccg cccggacggt ccgtgcgccg
     1501 tcacgatcgg gttcttcgac ggcgtccacc gcggccacca gacggtcatc cgccgcctag
     1561 tggccgcggc cgaggagcgg gacctccggc cggtggccgt caccttcgac cgccacccac
     1621 gcgaggtcct cacgccgggc gacgtgcccg gcctcctgac caccaccgat cggaaggccc
     1681 acctgatcga ggagctcggg gtcgacctcc tcgtcgtgat gcccttcgac gccgaggtgg
     1741 cctcgtggcc cccggagcga ttcgtggacg acgtcctcgt ggagcggctg ggcgtggcgc
     1801 acgccaccgt cggggcgaac ttcaccttcg ggcaccgggc cgccgggacg gtcgaggtcc
     1861 tgaccgacct ggggcgagcc cgggggttct ccgtcgaagg cgtgggcctt ctcgaggtcg
     1921 agggacgcag gatctcctcg acctcgatcc gcgaggccct ggctgccggc gacctggagt
     1981 ggccccgggt cgcgctcggc cgccgtcacg tgatcgacgg ggtcgtcgtg ccgggggcgg
     2041 gccggggcga cgacctggga tggccgacgg cgaacctcca gaccgacccc cgcgcgctgc
     2101 tgccggcgcg cggcgtctac gctggccgcg gcctgctgga ggagggcgcc tggcccgccg
     2161 cgatcaacgt ggggatcaac cccacgttcg gcgaggagcc cctccacgtc gaggcgttcc
     2221 tgctgggctt cgagggagac atccggggcc gggatctcgc gatcgagctc tgggagcggc
     2281 tccgcgacga ggagcgcttc gagtcgtccg aggctctctc gcgccagatc gagcgggacg
     2341 tggagcggac gcgggccgtc gtgtccgagt agtccccccg gagcccgaga ggccgtgtgc
     2401 taccctggtc gacggatgat cacgaaggaa gataagcaag ggctcatcgc cgagtaccgg
     2461 cagggcgagg gcgacacggg gtcgcccgag gtccaggtgg cgctcctcac caagcggatc
     2521 gaggagctga acgagcatct caagacccac cggaaggacc atcactcgcg ccgcggcctt
     2581 ctcctgatgg tggggaagcg ccgacggctc ctcgagtacc tcaagcgcga cgatatcgag
     2641 cggtaccgcg cgctgatcgg ccggctcggg ctccggcggt aggagggagc tcctaccgca
     2701 cctcgagcag cccacgaact cgaggaggaa ccatggaacc catcaccgtc tccggcaagg
     2761 tcggaggcac cgacctgtcg ttcgaaaccg gccgtctcgc gaagctcgcg cacggcgccg
     2821 cggtcgtgac cctgggggag acccaggtgc tcgtcacgtg cgcctggggc gccccgcggg
     2881 agggcatcga cttcttcccg ctgaccgtgg acgtcgagga gcggatgtac gccgcgggga
     2941 agatcccggg cggcttcttc cgccgcgagg ggaggccgtc ggagcaggcg atcctcacgt
     3001 gccgcctgac cgaccgaccg ctccgcccga cgttccgcga gggcttccgt caggaggtcc
     3061 acgtggtctc gacgatcctc tcggcggacc aggccaaccc ctacgacatc atcacgatca
     3121 acggcgccag cgccgcggtc atgctggcgg ggctgccgtt cgacggcccg atcggggccg
     3181 tgcgcgtggc catgatcagc ggcagctggg tggccaaccc cacctaccag gacctcgacc
     3241 tcgcgacctt cgacatggtg gtcgcgggcc gccggaacga cgcgggcggg acggacatcc
     3301 tcatggtgga ggcggaggcg cccgaggagg cgctctcgca catgtccgag ggccagccgg
     3361 ccccgaccga ggaggtcgtg gccgagggcc tcgaggaggc caagcgcgcc atcggcgagg
     3421 ccatcgacct gcaggaggag ctcgtccggc gggcgggggt ggagccgaag gagttccccg
     3481 tgacggtgga ctacgaggac gaggtcatgg aggccgtcca ggaggtcgcg ggatcgggcc
     3541 tctccgacgc gctgacgatc gccgacaaga ccgagcgcaa cgagcggctg gacgcgatca
     3601 aggaggacct cgggacgagc ctcaccgagc ggtggggcga ggagacctac gaggagaggg
     3661 cccggcaggt caaggccgcg ttcaaggccc tccagaagga gatcatgcgg aagcgggtga
     3721 tcgaggaggg gatccggatc gacgggcgga agcccgacga gatccgcccg atctcctgcg
     3781 ccgtggacct cgtgccgcga gcccacggct cgggcctctt cgagcggggg gaaacccagg
     3841 tcctgaacgt ggccacgctg ggcatgctgc ggatgacgca gatgatcgac accctcgacc
     3901 ccgaggagga gaagcggtac atgcaccact acaacttccc gcccttctcc acgggggagc
     3961 ccggcttcat gcgcggcccg aagcgccgcg agatcgggca cggggcgctg gccgaacggg
     4021 ccctcgcatc cgtgatcccc agcgaggagg agttcccgta cgccctgcgg ctcgtctccg
     4081 acgtgctggc gtcgaacggc tccacctcga tggcctcggc gtgcgcctcg acgctctcgc
     4141 tgatggacgc gggggtgccg atcaaggccc cggtggccgg gatcgccatg gggctgatcg
     4201 tcgagggcga tcgcttcgtg acgctgaccg acatcctggg cgccgaggac gccctcggcg
     4261 acatggactt caaggtggcc gggacgaagg acttcatcac ggcgctccag ctggacacga
     4321 aggtcaccgg cctcccggcc gaggtcctca gcggcgccct gaagcaggcc cgcgaggcga
     4381 ggctcttcat cctcgaccgg atgctggaga cgatccccga gccgcggtcc gcgctgtcgg
     4441 ccaaggcgcc gcgcgtggag gcgatccaga tccccgtgga caagatcggc gaggtcatcg
     4501 ggccgaaggg gaagcggatc aacgagatca tcgccgagac cggggccgac gtggacatcc
     4561 acgacgacgg cacggtcttc atcggctcca aggagggcga gggggccgag cgggccaagg
     4621 agatgatcaa cgcgatcgtc aac
//
LOCUS       DASUTW010000234         2415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_850663, whole genome shotgun sequence.
ACCESSION   DASUTW010000234 DASUTW010000000
VERSION     DASUTW010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850663"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VF058_08005"
     CDS             <1..316
                     /locus_tag="VF058_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX6208283.1"
                     /translation="GRRPTIPNHSVCLDCKRAGHVCVLVAHGTPCLGPVTHTGCGALC
                     PGYSRGCYGCFGPMETPDTTALSGRLRDLGMSEGDLVRVYRTFNTNAPAFKEESERHD
                     AS"
     gene            303..1598
                     /locus_tag="VF058_08010"
     CDS             303..1598
                     /locus_tag="VF058_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEX6208284.1"
                     /translation="MTHRDRTLHVAALARVEGEGAMLVRLEGGRVAEVQLNIYEPPRF
                     FEAFLRGRGHTEPPDITARICGICPVAYQMSACRAVEAAAGVEVPEQIHRLRRLLYCG
                     EWIESHALHIYLLHAPDFLGYHGAVEMAKDHREIVERGLGLKKVGNEVLELIGGRAIH
                     PINVRVGGFYRAPTHRELGPLAANLERAREAALETVRWVAGFDFPHFEQDYETVSLVH
                     PDVYPILEGQIGSSGGLDVAVAEYEEHFEEVHVPHSTALHARIRGGGAYLAGPIARYN
                     LNRDRLSPLALQAAREVGLEREVRNPFRSIIVRAVETLYACDEALRLIEGYEEPDEPF
                     VEVPARAGTGAGATEAPRGLLYHRYVTDEEGLIREAKIVPPTSQNQAQIEDDLRRFVE
                     PRIEMDTERLTWECEQAIRNYDPCISCATHFLTLEVRGG"
     gene            1595..2074
                     /locus_tag="VF058_08015"
     CDS             1595..2074
                     /locus_tag="VF058_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEX6208285.1"
                     /translation="MTDAVVIGVGNEFARDDAAGIEVVRRLWAEAPLGVEVVEHDGEP
                     ARLMELWAGRKRAWVVDAVRATDGSPGTVHRLEIGAEPIPDRPRRDSTHHLGLGDAVE
                     LSRAMGRLPGRLVLIAIEGAWFDTGVGMTPAVTDAVERVAGELRREVRASARTGGGR"
     gene            2074..2304
                     /locus_tag="VF058_08020"
     CDS             2074..2304
                     /locus_tag="VF058_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX6208286.1"
                     /translation="MCLALPARVIEVDPGGRSARVEVEGRTLEVSLDLLAVQGIEVRP
                     DGWLLVSAGAALEALDPATAEDLRTGWEERAP"
BASE COUNT          343 a          837 c          919 g          316 t
ORIGIN      
        1 cggccgccgg cccacgatcc cgaaccacag cgtctgcctg gactgcaagc gggccggcca
       61 cgtgtgcgtc ctggtggcgc acggcacgcc gtgcctgggg ccggtcacgc acacgggctg
      121 cggagccctc tgccccggct attcgcgggg ctgctacgga tgcttcggcc cgatggagac
      181 gccggatacg acggcgctca gcgggaggct ccgggacctg ggcatgtccg agggggacct
      241 cgtccgcgta taccggacct tcaacacgaa cgccccggcc ttcaaggagg agagcgagcg
      301 gcatgacgca tcgtgaccgg acgctccacg tcgcggcgct ggctagggtc gagggcgagg
      361 gggccatgct cgtgcggctc gagggcggcc gcgtggccga ggtccagctg aacatctacg
      421 agccgccgcg gttcttcgag gcgttcctgc ggggccgggg gcacaccgag ccgcccgaca
      481 tcaccgcccg catctgcggc atctgcccgg tggcctacca gatgagcgcg tgccgggcgg
      541 tcgaggcggc ggccggcgtg gaggtgcccg agcagatcca ccgcctccgg cggctgctct
      601 actgcggcga gtggatcgag agccacgccc tccacatcta cctgctccac gccccggact
      661 tcctcgggta ccacggcgcg gtggagatgg cgaaggacca ccgcgagatc gtggagcgag
      721 gcctcgggct gaagaaggtc gggaacgagg tgctggagct gatcggaggg cgagcgatcc
      781 atccgatcaa cgtccgggtg ggcgggttct accgcgcccc cacccaccgc gagctcgggc
      841 cgctggcggc gaacctggag cgggcccggg aggccgccct ggagacggtt cgctgggtgg
      901 ccggcttcga cttcccccac ttcgagcagg actacgagac ggtctcgctg gtgcacccgg
      961 acgtctatcc gatcctggag gggcagatcg gctccagcgg ggggctggac gtcgccgtgg
     1021 ccgagtacga ggagcacttc gaggaggtgc acgtgcctca ctccaccgcc ctccacgcga
     1081 ggatccgggg aggcggcgcg tacctggccg gccccatcgc ccgctacaac ctcaaccgcg
     1141 atcgtctctc gccgctcgcg ctccaggccg cccgggaggt cggcctggag cgcgaggtgc
     1201 gcaacccctt ccgcagcatc atcgtgcggg ccgtggagac gctctacgcg tgcgacgagg
     1261 ccctgcggct gatcgagggc tacgaggagc ccgacgagcc gttcgtcgag gttccggccc
     1321 gggcgggcac gggagccggc gccaccgagg ccccccgggg gctcctgtac caccggtacg
     1381 tcacggacga ggagggcctg atccgtgagg ccaagatcgt gccgccgacc tcccagaacc
     1441 aggcccagat cgaggacgac ctccgccgct tcgtcgagcc gcggatcgag atggacaccg
     1501 agcgactgac gtgggagtgc gagcaggcga tccgcaacta cgacccctgc atctcctgcg
     1561 ccacccactt tctcaccctg gaggtccgcg ggggatgacc gacgccgtcg tgatcggcgt
     1621 ggggaacgag ttcgcgcgcg acgacgccgc ggggatcgag gtggtccgcc gcctgtgggc
     1681 ggaggccccg ctgggggtcg aggtggtgga gcacgacggg gagcccgcgc ggcttatgga
     1741 gctctgggcc ggacggaagc gggcctgggt cgtggacgcg gtgagggcga cggacggctc
     1801 gccgggcacg gtgcaccgcc tggagatcgg ggccgagccg atcccggacc ggccccgccg
     1861 cgacagcacg caccacctcg gcctgggcga cgccgtcgag ctgtcccggg ccatggggcg
     1921 gctccccgga cggctcgtcc tcatcgccat cgagggagcg tggttcgaca caggcgtcgg
     1981 gatgacgccc gccgtgaccg acgcggtgga gcgggtcgcg ggcgagctcc gccgagaggt
     2041 gcgcgcctcg gcccgcacgg ggggcggtcg ctgatgtgcc tcgccctccc cgcgcgggtg
     2101 atcgaggtgg atccgggcgg ccgctcggct cgggtcgagg tcgagggccg caccctcgag
     2161 gtctcgctag acctcctggc cgtccagggg atcgaggtgc ggccggacgg ctggctgctc
     2221 gtgagcgccg gcgccgccct cgaggcgctg gacccggcca ccgccgagga tctgcgaacg
     2281 ggatgggagg agcgcgcgcc atgatgagga acgacacgag agcgatcggg atcggcctcg
     2341 ccctcgcgac ggcctgcatc agcggggtcg ccgtcttcgt gaacggctac ggggtcgcgg
     2401 ccttcgagga cgcca
//
LOCUS       DASUTW010000235         3628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1240876, whole genome shotgun sequence.
ACCESSION   DASUTW010000235 DASUTW010000000
VERSION     DASUTW010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240876"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..164
                     /locus_tag="VF058_08025"
     CDS             9..164
                     /locus_tag="VF058_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX6208287.1"
                     /translation="MAYGATTKEVATQLGISPHTVKTHLERIFEKLGANDRAQAVAIA
                     IRQGLVQ"
     gene            220..897
                     /locus_tag="VF058_08030"
     CDS             220..897
                     /locus_tag="VF058_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208288.1"
                     /translation="MLRRISLLLLLAVGVAASITGGPALSPPAEARPVREVAAGRVSA
                     LVPQKWDFRPMRSEVRAGEGIQASGDLRRWGSLERREIGLEAYWVDAAKIGLPSDYYY
                     LAAEGPAMTRLPVGVGCRQDRVDILSDHRPAFDRRRFSPGDYVATAGGTCVTRRGVTR
                     WASFIAAPGYGPVRRIGIPESGLYFAFVMVPDGPRADRRAERLLSAVSFGGTEVAEFI
                     AAARGQL"
     gene            complement(894..1970)
                     /gene="gcvT"
                     /locus_tag="VF058_08035"
     CDS             complement(894..1970)
                     /gene="gcvT"
                     /locus_tag="VF058_08035"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX6208289.1"
                     /translation="MEQALQHTPLEEEHRALGAALAPFGGWLMPIEYEGVLKEHAAVR
                     ERVGIFDLTHLGKVEVRGPRVLRHLQRILTNDLSKVAVGEARYTLVLTERGGIVDDLI
                     AYRTAEEEWMIVPNAGNTARVLGILEDGDADVRHRPDLVTLAVQGPEAIGLVAPSFPE
                     VEGLSFMEVAASSFEGTEVLVARTGYTGEPGVELFVPEDAGTALWRRLLERGATPVGL
                     GARDTLRLEMGYPLHGNDISEDRTPLEAGLSWAVAMDKGDFTGREALARIKEEGGPSE
                     RLRGLRMLGRRIPRPHFPVSKDGRPVGETTSGTFSPTLRVGIAMAYLDRGVEPGDTVE
                     VDVRGKGAEAEVVRPPFVDRSPKA"
     gene            2072..3460
                     /gene="lpdA"
                     /locus_tag="VF058_08040"
     CDS             2072..3460
                     /gene="lpdA"
                     /locus_tag="VF058_08040"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX6208290.1"
                     /translation="MADADVVVIGAGTGGYSCALRAAQLDKKVVLVERDDRVGGTCLL
                     RGCIPTKAILESAATMDRINRAEEWGIKASGEPDWAGVRAFEDAIVDKLVSGVAGLLK
                     ARKVEVVQGTAKLTGPGKIEVDTADGKRSLEAPDVVVATGSQPKLLPGMELGKRVITS
                     DQALFADSLPKSVVVIGAGAVGLEFASLYRSFGADVTLIEALPRLAPLEDEDISKEVD
                     RAFRKRGIATHAGAKVQDVKETQKQVTVTFETAKGETETAKAEVCLVAVGRGPISDGV
                     GLADVGVEMERGYVKVDGQLRTSVNGVWAVGDVAATPYQLAHVSMAEGIAVAERIAGL
                     DVPELDYAGVPRVTFSSPEIASVGLTEEQAKEHGHEVETDTLSFRGIGKANIVGEGGS
                     VKVVAEKDGPVLGVHMVGPHVTDLVAEAMLITNWEAIPAEVAALIHPHPTLSEGIGEA
                     HLALAGKPLHTM"
     gene            3534..>3628
                     /locus_tag="VF058_08045"
     CDS             3534..>3628
                     /locus_tag="VF058_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX6208291.1"
                     /translation="MAEPVTMPQLGETVVEGTIVKWLKSEGDSVDR"
BASE COUNT          522 a         1295 c         1308 g          503 t
ORIGIN      
        1 tccagaaggt ggcctacggg gccaccacca aggaggtcgc cactcagctc gggatctccc
       61 cgcacaccgt caagacccac ctcgagcgca tcttcgagaa gctcggggcg aacgaccggg
      121 cgcaggccgt ggccatcgcc atccgccagg gcctcgtcca gtagggtcta gccgcgcgtc
      181 catccctccc ctacactcgg cccaaggggg gaacctcgga tgctgcgccg gatctcgttg
      241 ctgctcctcc tcgccgtcgg cgtcgcggcg agcatcacgg gcgggcccgc cctcagccct
      301 ccggccgagg ctcgccccgt ccgggaggtg gcggccgggc gcgtctccgc tctcgtcccc
      361 cagaagtggg acttccggcc catgcgctcg gaggtccgtg cgggcgaggg gatccaggcg
      421 tcgggggacc tgcggcggtg gggatcgctg gagcggcgtg agatcgggct cgaggcctac
      481 tgggtcgacg ctgccaagat cgggctcccc tccgactact actacctggc cgccgagggc
      541 cccgcgatga cgaggctccc cgtcggcgtg ggctgcaggc aggaccgggt cgacatcctc
      601 tccgaccatc gacccgcctt cgatcgtcgg cgcttctcgc ccggcgacta cgtggccacg
      661 gccggcggca cgtgcgtcac gcggcgaggc gtcacccgct gggcctcgtt catcgccgcc
      721 cccggctacg ggccggttcg ccggatcggg atccccgaat ccggcctcta cttcgccttc
      781 gtcatggtcc ccgacggacc gcgagcggat cggcgcgcgg agcgcctgct gtctgccgtc
      841 tccttcgggg ggaccgaggt ggcggagttc atcgcggcgg cccggggtca gctctaggcc
      901 ttcgggctgc ggtccacgaa gggcgggcgg acgacctcgg cctcggcacc cttcccccgg
      961 acgtcgacct ccaccgtgtc gcccggctcg accccacgat cgaggtatgc catggcgatc
     1021 cccacccgca gggtgggcga gaacgtgccg ctcgtggtct cccctaccgg acggccgtcc
     1081 tttgacacgg ggaagtgggg ccgggggatg cgccggccga gcatccggag gccccggagg
     1141 cgctcgctcg gcccgccctc ctccttgatc cgcgccagcg cctcgcggcc ggtgaagtcg
     1201 cccttgtcca tcgccaccgc ccaggacagc ccggcctcga gcggggttcg gtcctcggag
     1261 atgtcgttcc cgtggagcgg gtagcccatc tccagacgga gggtgtcccg cgccccgagg
     1321 cccaccggcg tcgcaccgcg ctccagcagg cggcgccaga gggccgttcc cgcgtcctcg
     1381 gggacgaaca gctcgacgcc gggttcgccc gtgtagcccg tcctggccac gagcacctcg
     1441 gtgccctcga acgacgacgc ggccacctcc atgaacgaga ggccctccac ctccgggaag
     1501 gacggcgcca cgagcccgat cgcctccggg ccctggaccg ccagcgtcac gaggtcggga
     1561 cggtgccgca cgtccgcgtc gccgtcctcg aggatcccca ggacccgcgc cgtgttgccc
     1621 gcgttgggta cgatcatcca ctcctcctcg gcggtccggt aggcgatgag gtcgtcgacg
     1681 atcccgcccc gctcggtgag cacgagcgtg taccgggcct cccccacggc caccttcgac
     1741 aggtcgttcg tgaggatcct ctggaggtgc cggagcacgc gggggccccg cacctcgacc
     1801 ttcccgagat gcgtgaggtc gaagatcccg acccgctccc ggaccgccgc gtgctccttc
     1861 aggacgccct cgtactcgat cggcatcagc cagccgccga acggcgccag cgcggccccc
     1921 agcgctcgat gctcctcctc cagcggcgtg tgctgcagcg cttgctccaa tgggctcctc
     1981 ggtgcgtgga cgggtcgaac ggtagtatga cgggtgcggc ccgcggcggg cccccgtccg
     2041 ggccccaggc caacgacgaa aggatcttgg catggccgac gcagacgtcg tcgtgatcgg
     2101 cgcaggcacc ggcggctact cgtgcgccct ccgagccgcc cagctcgaca agaaggtcgt
     2161 gctggtggag cgggacgacc gggtcggggg gacctgtctc ctccgcggct gcatccccac
     2221 gaaggcgatc ctcgagtcgg cggccacgat ggacaggatc aaccgggccg aggaatgggg
     2281 catcaaggcc tccggcgagc ccgactgggc cggcgtccgg gcgttcgagg acgcgatcgt
     2341 cgacaagctc gtgagcggcg tcgccggact gttgaaggcc cgcaaggtcg aggtcgtgca
     2401 gggcaccgcg aagctcacgg gtccgggcaa gatcgaggtc gacaccgctg acggcaagcg
     2461 gtcgctcgag gcgcccgacg tcgtggtcgc caccggctcc cagcccaagc tgctccccgg
     2521 gatggagctc ggcaagcgcg tgatcacgag cgaccaggcg ctgttcgcgg actccctccc
     2581 aaagtcggtg gtcgtgatcg gggccggcgc ggtcggcctc gagttcgcct cgctctaccg
     2641 ctcgttcggg gcggacgtca cgctgatcga ggcgctcccc cgtctcgcgc cgctggagga
     2701 cgaggacatc tccaaggagg tggaccgcgc gttccggaag cggggcatcg cgacgcacgc
     2761 cggtgcgaag gtccaggacg tcaaggagac gcagaagcag gtcacggtga cgttcgagac
     2821 ggcgaagggc gagaccgaga ccgccaaggc cgaggtctgc ctcgtcgccg tggggcgggg
     2881 tccgatctcg gacggcgtcg ggctcgcgga cgtcggcgtc gagatggagc gcggctacgt
     2941 caaggtcgac gggcagctgc gcacgagcgt gaacggggtc tgggcggtgg gcgacgtcgc
     3001 ggccaccccc taccagctgg cccacgtctc gatggccgag gggatcgcgg tcgcggagcg
     3061 gatcgcggga ttggacgtcc ccgagctcga ctacgcgggc gtgccccggg tgacgttctc
     3121 cagcccggag atcgcgtcgg ttggcctcac cgaggagcag gccaaggagc acggtcacga
     3181 ggtcgagacc gacaccctct cattccgggg catcggcaaa gccaacatcg tgggcgaggg
     3241 ggggtcggtg aaggtcgtgg ccgagaagga cggccccgtc ctcggcgtgc acatggtcgg
     3301 cccgcacgtg accgacctcg tggccgaagc gatgctcatc acgaactggg aggcgatccc
     3361 cgccgaggtg gccgccctga tccaccctca ccccacgctc tcggagggca tcggggaggc
     3421 gcacctggcg ctcgccggca agccgctgca cacgatgtga agggcgcgtc ccggttgggg
     3481 gccggggcgc ggtggtacaa caacgcgagg acgacacgag gaggtcggga gacgtggccg
     3541 agccggtgac gatgccgcag ctgggcgaga ccgtcgtcga ggggacgatc gtcaagtggc
     3601 tgaagagcga gggggacagc gtcgaccg
//
LOCUS       DASUTW010000236         4727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_334422, whole genome shotgun sequence.
ACCESSION   DASUTW010000236 DASUTW010000000
VERSION     DASUTW010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334422"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VF058_08050"
     CDS             <1..579
                     /locus_tag="VF058_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX6208292.1"
                     /translation="TERGRERFRGIERADSVTLDPHKGMFLPYGTGCLLVREGRRLLD
                     AHATGEARYLQDLALEGESLNFSDYSPELSRDFRGLRVWLPLKLHGWEAFREALDEKL
                     DLARLLHEGIRDAPGIEVPWEPELSIVAFRSTDGEEASKEVLERINASQQIFLSSTMI
                     KGRFTLRAAILSFRTHRDRIEEAIEIIRSAAG"
     gene            complement(593..1321)
                     /locus_tag="VF058_08055"
     CDS             complement(593..1321)
                     /locus_tag="VF058_08055"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX6208293.1"
                     /translation="MEPLVAIGGRRLRPGSVGKWPDRGAAAVPDLFVQALHRAGAIEA
                     ILMPVEIDVDRAHAILDRFDALVLSGGGDIHPSRYGGEDHHKLWGVQAERDAFDLALA
                     RAAVERAMPFLAVCRGMQALNVALGGTLDPHLPDREGMIAHRKPGPDDDVLHDVRLEP
                     GTRTALAAGAELVAGASSHHQGVEKLGEGLVATGWSDDGLVEAVEHEDGWTVGVQWHP
                     EVTAGYDPANQGLFDVLVREAAGR"
     gene            complement(1331..2290)
                     /locus_tag="VF058_08060"
     CDS             complement(1331..2290)
                     /locus_tag="VF058_08060"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX6208294.1"
                     /translation="MTPPPVLVTRPLAEEILAFLRERCEVEVPGEDRAMPRDELLDRL
                     PGKHGALTMLTDRIDDEALDAAGPSLRVLANHAVGYDNVDVAACTRRGVIVTNTPDVL
                     TDATADMTWGLILSACRRIAEGDRLVRTGTPWEWSPGFMLGHEVTGKTLGIVGLGRIG
                     RAVARRAAGFEMRVLVHTRTPPGDTQVVGLDELLTESDVVSIHLPLNEETRHRIGARE
                     LGLMKETGVLVNTSRGPVVDEAALADALRAGRIAAAGLDVYEEEPRIHQRLLELENTV
                     LAPHLGSATVDTRTAMGMLAARNLTAVLSGLPPPTPINPEALG"
     gene            complement(2287..2955)
                     /locus_tag="VF058_08065"
     CDS             complement(2287..2955)
                     /locus_tag="VF058_08065"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX6208295.1"
                     /translation="MTRQHRRPAPRMRDLPAPRKNPSLAGPLFRTVWAWPYRFALAGL
                     HRMGVRPWQLTLLSLINNAICGWLIVSGRHFLGGILLLPAGLFDVFDGGVARLRGEES
                     RKGALLDAVNDRLADGIVFGAILYSLVDQGLTGAGKLALAALVVSLFVSHVRAEGEAR
                     GVDMSGGLFQRLERYIALMFGLTAPGMLAPVLAVLTGLGAFTILQRGVHAWRRLPRAE
                     RQAP"
     gene            3021..4439
                     /gene="zwf"
                     /locus_tag="VF058_08070"
     CDS             3021..4439
                     /gene="zwf"
                     /locus_tag="VF058_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX6208296.1"
                     /translation="MVVPALDPVEIVIFGASGDLTRRKLLPALFHLFIGDLMPEDFGV
                     VGYARTEMSDEAFREMALEAVREYARCDVDGETWGAFARRLHYVTGEFTEEGAFARLR
                     GRLDHLDEEHGTDGRRRLYYCGTPPVAYADIVRRLGEEGMAPGSRIVVEKPFGRDLET
                     ARDLNRILHESFDEEQIYRIDHYLGKETVQNILAFRFANSIIEPLWNRRYVDHVQLTV
                     AEDEGIEGRGAFYEATGAMRDMVQAHLFQVLTFLAMEPPSAFEPEPLRDEKMKVLRSM
                     RPVDPSAVVRGQYEGYREEEGVDPASEMETYATLRVEIDNWRWAGVPFFLRTGKRMAE
                     KRAEATIQFKEPPHLVFERAGIDADPNRLTLTIQPDEGISLSFMAQGPGVGMSLVPAS
                     LEFDYASAFDTELVEAYEVLLLEAMRGDRTLFLRADIVERAWELLEPVLEAPSQVVPY
                     EAGSWGPKDADDLIAPRNWFLA"
     gene            complement(4436..>4727)
                     /locus_tag="VF058_08075"
     CDS             complement(4436..>4727)
                     /locus_tag="VF058_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208297.1"
                     /translation="ILLRYAAHATQLARWDEATSSWTNLEATLLRGTLQIYGPSTELG
                     TFVALGPPTPPPQEEGWPFWARWALYAGAGVVVVLAVEVYLRWRRRRTEKSG"
BASE COUNT          683 a         1702 c         1687 g          655 t
ORIGIN      
        1 acggagcggg gccgcgagcg cttccggggg atcgagcggg ccgactcggt gacgctcgac
       61 ccgcacaagg ggatgttcct cccgtacggc acgggctgcc tgctggtccg cgagggccgc
      121 cggctgctcg acgcccacgc cacgggcgag gcccgctacc tccaggatct ggccctggag
      181 ggcgagtcgc tgaacttctc cgactactcg cccgagctct cgcgggactt ccgcgggctc
      241 cgggtgtggc tgccgttgaa gctccacggc tgggaggcgt tccgcgaggc cctggacgag
      301 aagctcgacc tggctcgcct gctccacgag gggatccggg acgccccggg gatcgaggtg
      361 ccctgggagc ccgagctctc gatcgtcgcg ttccgttcca cggatggcga ggaggcgagc
      421 aaggaggtgc tggagcgcat caacgcgtcc cagcaaatct tcctctccag cacgatgatc
      481 aaggggcgct tcaccctgcg cgcggcgatc ctctcgttcc ggacccaccg ggaccggatc
      541 gaggaggcga tcgagatcat ccgctcggcc gccggctgac gccggtccgc cgtcagcggc
      601 cggccgcctc gcggacgagc acgtcgaaga gaccctggtt ggcggggtcg tatcccgccg
      661 tcacctcggg gtgccactgg acgccgaccg tccacccgtc ctcgtgctcg acggcctcca
      721 cgagcccgtc gtcgctccag cctgtggcca cgagcccctc gccgagcttc tccaccccct
      781 ggtggtgcga ggaggcgccg gccacgagct cggctccggc cgcgagtgcc gtccgggtcc
      841 ccggctcgag ccgaacgtcg tgcagcacgt cgtcgtccgg ccccggcttc cggtgggcga
      901 tcatgccctc ccggtcgggg agatggggat cgagcgtccc gccgagggcg acgttcagcg
      961 cctgcatgcc ccgacagacg gcgaggaacg gcatcgctcg ctccacggcc gcccgggcca
     1021 gcgcgaggtc gaaggcgtca cgctccgcct ggacgcccca cagcttgtga tggtcctcac
     1081 ccccgtacct cgacggatgg atgtcgccgc ctccgctcag caccagcgcg tcgaagcggt
     1141 cgaggatggc gtgcgcccga tcgacgtcga tctcgacggg catgaggatc gcctcgatgg
     1201 cccccgctcg atggagggcc tggacgaaga ggtctggcac cgccgccgcc ccgcgatcgg
     1261 gccacttccc gaccgagccg gggcgcagcc ggcgccctcc gatcgcgacg agcggttcca
     1321 tgtccccagc ctagccgagc gcctcggggt tgatcggcgt ggggggcggc agtcccgaca
     1381 gcacggccgt caggttgcgg gcggccagca tccccatcgc ggtccgggta tcgaccgtgg
     1441 cgctccccag atgcggggcg agcacggtgt tctccagctc cagcagccgc tggtggatcc
     1501 tgggctcctc ctcgtagacg tcgaggccgg cggccgcgat ccgcccggcc cggagcgcgt
     1561 ccgcgagcgc ggcctcgtcc acgaccgggc cccgcgacgt gttcacgaga accccggtct
     1621 ccttcatcag gccgagctcc cgagccccga tgcggtggcg ggtctcctcg ttcagtggga
     1681 ggtggatcga gacgacgtcg ctctcggtca gcagctcgtc gaggccgacg acctgcgtgt
     1741 ccccgggcgg cgtccgcgtg tgcaccagca cccgcatctc gaagccggcc gctcggcggg
     1801 ccaccgcgcg gccgatccga ccgagcccaa cgatccccag cgtcttgccg gtgacctcgt
     1861 gtccgagcat gaagcccggg gaccactccc agggcgtccc ggtccgcacg aggcggtcac
     1921 cctccgcgat gcgacggcag gccgagagga tcagccccca ggtcatgtcg gccgtggcat
     1981 cggtcaggac gtcgggggtg ttcgtcacga tcacgccgcg ccgcgtgcag gccgcgacgt
     2041 ccacgttgtc gtagccgacc gcgtggttgg ccaggacgcg cagcgagggc cccgccgcgt
     2101 cgagggcctc gtcgtcgatc cggtcggtga gcatcgtcag cgcgccgtgc ttcccgggca
     2161 gccggtccag cagctcgtcg cggggcatgg cccggtcctc gcccgggacc tcgacctcgc
     2221 agcgctcccg caggaaggcg aggatctcct cggccaaagg gcgggtcacg aggacgggcg
     2281 gaggggtcac ggagcctgac gctccgcccg ggggaggcgc cgccacgcgt ggaccccccg
     2341 ctggaggatc gtgaaggcgc cgagccccgt gagcaccgcc aggaccgggg ccagcatccc
     2401 gggggcggtc aggccgaaca tgagcgcgat gtaccgctcc agccgctgga acagcccgcc
     2461 ggacatgtcc accccgcgag cctctccctc ggcccgcacg tggctcacga agagggagac
     2521 gacgagcgcc gcgagcgcga gcttccccgc ccccgtcagc ccctggtcca cgagcgagta
     2581 gaggatcgcc ccgaagacga tcccgtcggc cagccggtcg ttcacggcgt cgaggagtgc
     2641 gcccttcctc gactcctccc cgcgaagcct ggccaccccg ccgtcgaaga cgtcgaagag
     2701 cccggccggg agcagcagga tcccccccag gaagtgccgg ccggagacga tcagccaccc
     2761 gcagatcgcg ttgttgatca gcgaaaggag cgtgagctgc caggggcgga cgcccatccg
     2821 gtgcaggccg gccagggcga accggtaggg ccaggcccag acggtccgga agaggggccc
     2881 ggccaggctg gggttcttcc tgggcgccgg gaggtcgcgc atccgcggcg ccggccggcg
     2941 gtgctgtcga gtcatcggcg gcagtatccg tcgccggccg gcgccgcggc aacgagcggc
     3001 gcctgacata ggatggcgag atggtcgtgc ctgctctcga ccccgtcgag atcgtcatct
     3061 tcggcgcctc cggcgacctc acccgccgca agttgctgcc cgccctcttc cacctcttca
     3121 tcggggatct catgccggag gacttcggag tcgtgggcta cgcgcgcacc gagatgagcg
     3181 acgaggcgtt ccgagagatg gcgctggagg ccgtccgcga gtacgcgcgg tgcgacgtcg
     3241 acggagagac ctggggcgcg ttcgcccggc gcctgcacta cgtgacgggg gagttcacgg
     3301 aggagggcgc cttcgcccgg ctgcgcgggc ggctcgacca cctcgacgag gagcacggga
     3361 ccgacggccg ccgccgcctg tactactgcg ggaccccgcc ggtggcctac gccgacatcg
     3421 tccgcaggct gggggaggaa gggatggccc ccggctcccg catcgtcgtg gagaagccct
     3481 tcggccgcga cctggagacc gcccgagacc tcaaccggat cctccacgag tccttcgacg
     3541 aggagcagat ctaccgcatc gaccactacc tggggaagga gaccgtccag aacatcctcg
     3601 ccttccgctt cgccaactcg atcatcgagc ccctctggaa ccggcggtac gtggaccacg
     3661 ttcagctcac cgtggccgaa gacgagggca tcgagggccg aggggccttc tacgaggcca
     3721 ccggcgcgat gcgggacatg gtgcaggccc acctcttcca ggtgctgacc ttcctggcga
     3781 tggagccccc gtcggccttc gagccggagc ccctgcgcga cgagaagatg aaggtcctgc
     3841 gctcgatgcg gccggtggat ccctcggccg tggtccgcgg ccagtacgag ggctaccgcg
     3901 aggaagaggg ggtggatccg gcctccgaga tggagacgta cgccaccctc cgcgtcgaga
     3961 tcgacaactg gcggtgggcg ggcgtcccgt tcttcctgcg gacggggaag cggatggccg
     4021 agaaacgggc cgaggcaacg atccagttca aggagccccc gcacctcgtg ttcgaacgcg
     4081 cggggatcga cgccgacccc aaccgcctca cgctgacgat ccagccggac gaggggatct
     4141 cgctgtcgtt catggcgcag gggccgggcg tggggatgtc gctcgtaccc gcctcgctgg
     4201 agttcgacta cgcctcggcc ttcgacaccg agctggtgga ggcctacgag gtgctgctgc
     4261 tcgaggcgat gcggggcgac cggacgctgt tcctccgggc cgacatcgtg gagcgggcgt
     4321 gggagctgtt ggagccggtg ctggaggcgc cgtcgcaggt cgttccgtac gaggcgggct
     4381 cgtggggtcc gaaggatgcg gacgacctga tcgcgcctcg gaactggttc ctggcctaac
     4441 ctgacttctc ggtccgccgg cgccgccacc gcaggtagac ctccaccgcc aggacgacca
     4501 cgacgccggc ccccgcgtac agtgcccagc gggcccagaa gggccacccc tcctcctgcg
     4561 gcggcggcgt aggcgggccc agggcgacga acgtcccgag ctccgtagaa ggcccgtaga
     4621 tctggagggt tcctcgcagc agggtggcct ccaggttggt ccacgagctc gtggcttcgt
     4681 cccaccgcgc cagctgggtg gcgtgggccg cgtagcggag caggatc
//
LOCUS       DASUTW010000237        15753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1500487, whole genome shotgun sequence.
ACCESSION   DASUTW010000237 DASUTW010000000
VERSION     DASUTW010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15753
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500487"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1191)
                     /gene="rodA"
                     /locus_tag="VF058_08080"
     CDS             complement(49..1191)
                     /gene="rodA"
                     /locus_tag="VF058_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX6208298.1"
                     /translation="MALEAAPARRWLGEVAPIRHLDPTLIFVSIGLTIYGLLMVYSAT
                     HRTLATFGEDPGFYLKRQLAFMMLGLIVLVIASVFDYRLVKVYAPFIFAICGFLLFLV
                     LTPLGSETAGAQRWLSLFGFQFQPSEVAKLALIAMLAAYLSEIRGPVRLEHVWRATGL
                     TVIPMALVFLQPDIGTSMVFAVILVTMLIVSGAKAKQIGILAIAALIALFAAFRLGVV
                     QEYQIERLTGFLDPANDPLRAGYNRAQAEIAIGAGGLFGTGYGSGTQTNLDFVPEQHT
                     DFIFTVVGEEFGFVGAVLLLSMYGILVWRGFRIAMMSKDAFGSLLALGIAGMLAFQVF
                     VNVGMTLGIMPITGIPLPFLSYGGTALLTNFLAVGLLLNVHMRRFK"
     gene            complement(1191..3095)
                     /gene="mrdA"
                     /locus_tag="VF058_08085"
     CDS             complement(1191..3095)
                     /gene="mrdA"
                     /locus_tag="VF058_08085"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX6208299.1"
                     /translation="MSEDRMSNRMRVLAAFVAFLFAALVTRLWFLQVLAAEEYQERAE
                     SNRVRIIPEPAPRGRILDANGRVLVDNRRSIQVVVNRRHVENPEELLFRLSEVLEVPV
                     NELAERYEDPTYLPYQPVPVADDVPERKAAYLVAHRREFPGVSYRVVGVRDYVHGSLA
                     AHVLGYLGEINEEELKSPAFRRYRPGDEVGRGGVEQHYEEFLRGKEGYRSIQVDAQGR
                     SGQVLGSRRPEPGNDLYLSIDLGLQTAAEESLAQGVRAANGVVTSEGRTLNAKAGAVV
                     ALDPNTGHVRAMASFPAYNPEVFVDGLSQREWRRFDRDPTHPLNNRAIQGLYPAASTF
                     KPFVAAAAVKAGIAGPEGYFPCPPTFEVPGDTSGTVFHNWHSGNDGFLSLAGSLERSC
                     DTVYYQFGLDFWRLRDERGDVMQRQLRKWGFGRPTGVDVPGELEGRVPDSEWKQQVHE
                     LYPKYFPEGLWLPGDNINMSIGQGDLLVTPLQLASAYAAIANGGTLYRPEVGLRVEAP
                     DGTVLKAVAPREIRRIPMQKKAREQVLAGLRAAVQGVGGTATTAFAGFPGSVAGKTGT
                     AEVIVDGQETTHSWFAAFAPAEDPEILVVAIVEEGGHGSQVAAPIVRRILEQYFGITP
                     SAFRIGVATD"
     gene            complement(3097..3603)
                     /gene="mreD"
                     /locus_tag="VF058_08090"
     CDS             complement(3097..3603)
                     /gene="mreD"
                     /locus_tag="VF058_08090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX6208300.1"
                     /translation="MRRGLAWAAVVVTALLLQSTVFAQVKLAGAKPELMFLITIVMAM
                     LEGPSVGAVTGFTAGMGQDFLLDQPKGLTALTLTLVGYAVGQVRQYIVTPGPLTPVLL
                     VGGFTAIGVLFYGLVAFLLGQLGTGFLFLLRTALLSAVYNAVLTPLLYPVLKRVAEAS
                     KTSRVHRW"
     gene            complement(3643..4488)
                     /gene="mreC"
                     /locus_tag="VF058_08095"
     CDS             complement(3643..4488)
                     /gene="mreC"
                     /locus_tag="VF058_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX6208301.1"
                     /translation="MAIYRRERSTRLLVVALVVTSLVIITLDIRGGQRGPLAAAGTIS
                     LANISPLQDGIATVFRPIGSFFSNLASLGSLRAENERLQAEVAELQAVRVRFLEYERR
                     LKQLTALLEFKETQEVETIGATVISEVPTNFEASVTIDRGTADGVELDMAVVAAEGLV
                     GRVVSVSERWSKVLLLIDVDHAVHARLASTGETGILRGQRERDLRFDLIDPGTPVEAG
                     EPVVTSGQDGVYPPGIEIGAVSHVAPNESGFQEQILVRPYVDVSRLSEVLVVLSGGVV
                     EGSQR"
     gene            complement(4516..5538)
                     /locus_tag="VF058_08100"
     CDS             complement(4516..5538)
                     /locus_tag="VF058_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX6208302.1"
                     /translation="MTGLLGRDMAVDLGTANTLVYVRGRGIVLNEPSVVAINTMTKQV
                     LAVGAEAKRMIGRTPSHIQAVRPLKDGVIADFDITEKMLRYFIQKVHTRRFLAKPRVV
                     VCVPSGITGVEQRAVEEATISAGARAAYIIEEPMAAAIGAGLPVHEPTGNMVVDIGGG
                     TTEGAIISLGGIVTATSVRVGGDELDEAIIQYVKKEYSLVLGERTAEAIKMAIGSAFP
                     MPDEMIAEIKGRDLVTGLPKTIHVSAEEIRKGLEEPVNAVVDAIKNTLDRCPPELAAD
                     IMDQGIVLTGGGALLKGLDERLKHETGMPIHVAENPLACVVMGSGKCLEEFEALKQVL
                     ASSTRR"
     gene            5919..6842
                     /locus_tag="VF058_08105"
     CDS             5919..6842
                     /locus_tag="VF058_08105"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX6208303.1"
                     /translation="MRIAVLFPGQGSQYAGMADPWTDHPRGKETLAEASDVLGYDVVA
                     VASDEEKLGLTDVVQPAMFACDLAALRVLEAEGATFAMTAGHSLGEFVALAAAGAVPV
                     DQALTAVVERGRAMQEASDTSPGGMTALVGVSPQEAAEIVATAGRGDVLTVANENSPR
                     QTVLSGSIPAIERAEELARSRKVRTVRLKVAGAFHSKLMEPALARVRAAIAKLDFSEP
                     RFPVVPNASGRPTSQPSALRDLLSRHLVSPVKWERSIRAMADAGIDLFVEAGPGDVLS
                     KLVKRSVPGSRAVPVGSPADARAFVEQEGVR"
     gene            6839..7129
                     /locus_tag="VF058_08110"
     CDS             6839..7129
                     /locus_tag="VF058_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208304.1"
                     /translation="MSITFEPFDHSPTTLRVLVSPGAGRLRYLPPLRFAGGRELVEAG
                     QPVARVLQGSGEVILRAPVGGVVSSILGIEGEPLVAGQAILAIEPELAPEGS"
     gene            7126..8130
                     /locus_tag="VF058_08115"
     CDS             7126..8130
                     /locus_tag="VF058_08115"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX6208305.1"
                     /translation="MTGRHAQIAGFGSALPERVVTNDDLAAMGVDTSDEWIRTRTGIG
                     ARRFVAEGETASMLGARAAREALEAAGVDPGAVDLTICATFTGDQPLPSTASFLQAEL
                     GIHGAAFDLAAACAGFIYATEMARGQIVTGGAETVLVVGVEVLSPILNLEDRTTCVLF
                     GDGAGAAVLVPGDEPGIIGSVLENDGRHAHILEGPSGGSASPISAETIARGEHFIRMA
                     DGREVFKRAVRGMSDAARRLLEKAGLALGDVTMLVPHQANARIIRAVAERLSVPPERV
                     YIDLEEVGNTSAASVPIALDHAWREGRLSPGDLVLTAAFGAGLAWGANLIRWTAEPPA
                     "
     gene            8127..8864
                     /locus_tag="VF058_08120"
     CDS             8127..8864
                     /locus_tag="VF058_08120"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEX6208306.1"
                     /translation="MSGRIALVTGAGGGLGRAAALELAGRGMPVACAYGTNDAGAKET
                     AEMIEERGGEAQAFGADVADEQEVRRLFREIKEWRGAPLVLVANAGVRRDGPTETYPL
                     EQWQRTLDVNLTGVFLCVREALRGMQLARWGRVILVSSAAGLRGNPGQASYSASKAGV
                     LGLTRSLSREVGARGITVNAVAPGYVETAIVAEMTEAARERMMGLIATGRPGRPEEIA
                     AAIGFLAGEEASYVNGAVLPVDGGMTA"
     gene            8896..9147
                     /gene="acpP"
                     /locus_tag="VF058_08125"
     CDS             8896..9147
                     /gene="acpP"
                     /locus_tag="VF058_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009556086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX6208307.1"
                     /translation="MERKEIEERVRKVLAEQLARDPDEVTLEASFEDDLDADSLDLVE
                     AVLALEEEFGIEIPEEEMEGVKTVKEAVDLAASKLGVSA"
     gene            9148..10377
                     /gene="fabF"
                     /locus_tag="VF058_08130"
     CDS             9148..10377
                     /gene="fabF"
                     /locus_tag="VF058_08130"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX6208308.1"
                     /translation="MTERKVVLTGIGPVTPVGTGREPFWDALVSGRSGIGEITLFDAS
                     EHPVRIGGEVEGFDAADWMDRKAAKRTDRAVHLAVAAAKLAWEDAGSPQIDPQRAAVV
                     VSTGVGGLRSMEEQIKILNERGPGRVSAFTVPAMMANAATGTVAMEFGFTGPNACITT
                     ACAAGAHGVGEGYRYIKHGMADLALAGGTEAGITPLSLAAFAQMQALSRNADPERASR
                     PFDADRDGFVISEAACVLVLEEEERARARGATIYAEVAGYGTSADAFHITQPEPQGAG
                     AVIAMQMALTDAGEPAEAVDYINAHGTSTPLNDAAETRAIKKAFGDYAYEVAVSSTKS
                     MTGHMVGAAGAAEAAAAALAVRDGVIPPTINHETPDPECDLDYVPNEKRETDVRLALS
                     NAFGFGGQNAVLALRRI"
     gene            complement(10378..11118)
                     /locus_tag="VF058_08135"
     CDS             complement(10378..11118)
                     /locus_tag="VF058_08135"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEX6208309.1"
                     /translation="MAISVAPKARIGARLREARVRRGVSVEDAALHTRIKPRFLRALE
                     ADAPPSAFRAPVYARAFLREYAGFLGLDPEPLVADYATAHGIGDGQPIRLPRPVERRS
                     HRKLIATLLWIASLGAMGALVVVSARSVERSIPPAPLPAEASPPAVEAGAPAGEEDEI
                     DLRLRVKDARTWLRVRVDGELAEDGRRKPGFVKTFRADRRIRLEVANAGAVRLRLGGE
                     PLGAPGAEGVTYRATFVLVDGEVQVRRP"
     gene            complement(11194..11598)
                     /gene="ndk"
                     /locus_tag="VF058_08140"
     CDS             complement(11194..11598)
                     /gene="ndk"
                     /locus_tag="VF058_08140"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010978231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX6208310.1"
                     /translation="MTTQETLVIAKPDAVRRRLVGEILRRFEERGLRITGLRMMRIDQ
                     DLADRHYEEHRDKPFFGELVEFITSGPVVVARIEGEDAVAAVRELMGPTDPKDAPPGT
                     IRGDYGEVITENLVHGSDSPENAKRELDLFFG"
     gene            complement(11600..12880)
                     /locus_tag="VF058_08145"
     CDS             complement(11600..12880)
                     /locus_tag="VF058_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX6208311.1"
                     /translation="MRFEDAVADLEARGEGRMIPTLDRIRAVVELMGDPQLAYPTVHV
                     AGTNGKTTTTRLVERLLCAHGVSAGALTSPHLHTVRERLTFCGDPIEEQEFAETYAHL
                     RHFLEEVDREGERVTYFETLAALAALWFADKPVDVGVFEVGMGGSWDATNVIRSEVAV
                     ITPIGLDHPELGSTVEEVAAEKAGVLKEGTRAVVREQRPEARKVLEARAAEVGATLLH
                     EGEDFTVPVRDLAVGGQRLAVRGVRGEYRGLSLPLHGEHAARSAAAAVAAAELTLDRE
                     LGANAVREALAGASSPGRLEVVARHPLVVLDGGHNPDGAAALAAAVGEAFIWERLYLV
                     LAVLETKDATGIASALAPRTTSAFVAASSHPRAMDPERLAAVAERAGIPAIAHPTVAA
                     ALDAATKAAGPDDLILVTGSLYTVADARPRYVKG"
     gene            complement(12882..15521)
                     /locus_tag="VF058_08150"
     CDS             complement(12882..15521)
                     /locus_tag="VF058_08150"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX6208312.1"
                     /translation="MTTASAYEPSEVESRLYADWIERGYFRAEPTEEREPFSIVLPPP
                     NVTGALHIGHALDHTIQDALIRRRRMQGYEALWLPGTDHAGIGTEVLVGRRLLEEGID
                     KQAIGREAFIERVWEWRERYGGRIVEQMKALGSSCDWDRLRFTMDEGLQHAVRVAFVR
                     LYEDGLIYRGERIINWCPTDQTALSDSEVEHEEVEGELVTFRYPMADGDGHIEVATTR
                     VETMLGDTGIAVHPDDERYQDLVGRSVRHPLDGRELPIVADETVDPSFGTGAVKVTPA
                     HDPNDFEIAERTGLEARNVLTSEARLNDAVPEAFRGLDRYEARRAVRDRLDELGLIVR
                     EERPYVHSVGHCYRCRSEIEPWLSGEQWFVKVDPLKGPAMEAAESGGLVFSPPRWKDR
                     YLEWLEGLRDWNISRQLWWGHRIPVWYCSNGHEFASIEDPKGCAQCGSAEIEQDPDVL
                     DTWFSSQLWPFSTLGWPEETEDLHYFYPTSVLVTGYEILYLWVARMVMSGLRLTGDLP
                     FHTVVIHGLVRDAQGRKMSKSLGNVIDPLDMIERYGADALRFALSRMASPEQQNLPLA
                     EDQIEAGKHFANKLWNAARLVRTNLEGDDLSPPSPERLTVTDRWLLSRVEACHAEVDA
                     AMETYRFADAAQALYRFLWSEYCDWGLEMAKPRFYEGSEEDRRDAAAVLAWVLERSLR
                     LLHPIMPFVTEEIRRRFVLEGSIMIASWPEPMPAHRDEEAEARVGFAMDLVTAVRRFR
                     SDHRVPPSLELRVRVEGDAAQRQILGTLSEEVRRLARVGSIVTTGEASSPSGSARLHV
                     QGAEVFIPLEGVLDPEAECDRLRSRIEALGEEAGKAERKLANEGFVTKAPAEVVEEQR
                     RRLTRLKEEEAALREQLDELGCG"
BASE COUNT         2347 a         5664 c         5489 g         2253 t
ORIGIN      
        1 acatgcacca ccaggatagc aggaggaggt gccggctccg ggaggctcct acttgaacct
       61 gcgcatgtgc acgttcagca ggaggcccac cgccaggaag ttggtgagca gcgcggtgcc
      121 cccgtaggag aggaacggta acgggatccc cgtgatcggc atgatcccca gggtcatccc
      181 cacgttgacg aagacctgga acgcgagcat ccccgcgatc cccagggcga gcagggagcc
      241 gaaggcgtcc ttcgacatca tggcgatcct gaacccgcgc cagaccagga tgccgtacat
      301 cgagaggagg aggacggccc ccacgaatcc gaactcctcg ccgaccaccg tgaagatgaa
      361 gtccgtgtgc tgctcgggga cgaagtcgag gttcgtctgg gtccccgacc cgtaccccgt
      421 cccgaacagt ccgccggcgc cgatcgcgat ctcggcctgc gcccggttgt agccggcgcg
      481 gagcgggtcg ttcgcggggt cgaggaaccc ggtcagccgc tcgatctggt actcctgcac
      541 cacccccagc cggaacgcgg cgaagagcgc gatcagtgcc gcgatcgcca ggatcccgat
      601 ctgtttcgcc ttcgcccccg agacgatcag catcgtcacg aggatcacgg cgaagaccat
      661 cgaggtcccg atgtcgggct ggaggaagac cagggccatc gggatcaccg tgaggccggt
      721 ggcccgccag acgtgctcca ggcgcacggg gccccggatc tccgagaggt atgcggcaag
      781 catcgcgatc agcgcgagct tcgcgacctc cgagggctgg aactggaacc cgaacaggct
      841 cagccagcgc tgggcgccgg ccgtctcgct gccgagcggg gtcagcacga ggaagaggag
      901 gaagccgcag atggcgaaga tgaacggcgc gtacaccttc accagccggt agtcgaagac
      961 cgaggcgatc acgagcacga tgagccccag catcatgaag gcgagctgcc gcttgaggta
     1021 gaagcccggg tcctcgccga acgtggccag cgtccggtgg gtggcggagt agaccatcag
     1081 caggccgtag atcgtcagcc cgatgctgac gaagatcagc gtggggtcga ggtggcggat
     1141 cggggccacc tcgccgagcc accgccgcgc cggagccgcc tcgagcgcca tcagtccgtc
     1201 gccaccccga tgcggaacgc gctgggcgtg atcccgaagt actgctcgag gatccggcgc
     1261 acgatcggag ccgccacctg cgagccgtgc ccaccctcct cgacgatggc aaccaccagg
     1321 atctccggat cctcggccgg ggcgaacgcc gcgaaccatg agtgggtcgt ctcttgaccg
     1381 tccacgatca cctcggccgt gccggtcttg ccggccacgg accccgggaa gccggcgaac
     1441 gccgtggtgg ccgtcccccc gacgccctgc acggccgccc gcaggccggc caggacctgc
     1501 tccctcgcct tcttctgcat ggggatcctt cggatctcgc gcggcgccac ggccttgagg
     1561 acggtcccgt cgggcgcctc cacgcggagg ccgacctcag gccggtacag cgtgcccccg
     1621 ttcgcgatcg ccgcgtaggc ggaggcgagc tgcagcgggg tcacgaggag gtccccctgc
     1681 ccgatcgaca tgttgatgtt gtcgccgggg agccagagac cctcggggaa gtacttcggg
     1741 tagagctcgt gcacctgctg cttccattcg ctgtcgggga cccgaccctc gagctctccc
     1801 gggacgtcga ccccggtggg ccggccgaac ccccacttcc ggagctgccg ctgcatcacg
     1861 tcgccgcgct cgtcccgcag ccgccagaag tccagcccga actggtagta caccgtgtcg
     1921 caggaacgct ccagggaccc cgccagcgag aggaaaccgt cgttccccga gtgccagttg
     1981 tggaacacgg tccccgacgt gtcgcccggc acctcgaacg tcggggggca cgggaagtag
     2041 ccctcggggc cggcgatccc cgccttcacc gccgcggcgg ccacgaacgg cttgaaggtg
     2101 gaggccgcgg gatagaggcc ctggatcgcc cggttgttga gcggatgggt cgggtcgcgg
     2161 tcgaaccgcc tccattccct ctggctgagg ccgtccacga acacctcggg gttgtaggcg
     2221 gggaacgagg ccatggcgcg gacgtgaccg gtgttcgggt ccagggcgac cacggctccc
     2281 gccttggcgt tgagcgttcg gccctccgag gtgacgaccc cgttggcggc cctcacgccc
     2341 tgggccaggc tctcctcggc cgccgtctgg agcccgaggt cgatcgagag gtacaggtcg
     2401 ttgccgggct ccgggcgacg ggagccgagc acctgcccgg accggccctg ggcgtcgacc
     2461 tggatgctcc ggtagccctc cttgccccgg aggaactcct cgtagtgctg ctcgacgccg
     2521 ccccgaccga cctcgtcgcc cggccggtag cgtcggaacg cgggtgactt cagctcctct
     2581 tcgttgatct ctcccaggta gcccagcacg tgggccgcga gcgacccgtg cacgtagtcg
     2641 cgcacgccga ccacgcgata cgagacgccg gggaactccc tgcggtgcgc cacgaggtag
     2701 gccgccttcc gctcggggac gtcgtcggcc acgggcaccg gctggtacgg caggtaggtg
     2761 gggtcctcgt atcgctcggc cagctcattc accggcacct cgagcacctc ggagagacgg
     2821 aagaggagct cttcggggtt ctcgacgtgg cgccggttga cgaccacctg gatgctccgt
     2881 cggttgtcca cgagaacccg accgttcgcg tcgaggatcc tcccccgcgg ggctggctcc
     2941 gggatgatcc ggacgcggtt ggactccgca cgctcctggt actcctcggc ggcgaggacc
     3001 tgaaggaacc acagccgagt caccagggcg gcgaacagga acgccacgaa cgcggcgagc
     3061 acccgcatcc ggttcgacat ccgatcctcg ctcatgtcac cacctgtgca cccggctcgt
     3121 cttggacgcc tcggccacgc gcttcagcac ggggtacagg aggggcgtca gcacggcgtt
     3181 gtagaccgcg gacagcagag ccgtccgcag caggaagagg aagccggtcc cgagctggcc
     3241 cagcaggaag gccacgagcc cgtagaagag cacgccgatc gccgtgaacc cgcccaccag
     3301 cagcacgggg gtgagggggc cgggcgtgac gatgtactgg cgcacctgcc ccaccgcgta
     3361 gcccacgagc gtgagcgtca gcgccgtgag gcccttgggc tgatcgagga ggaagtcctg
     3421 ccccatcccc gccgtgaacc ccgtgacggc gcccaccgac gggccctcga gcatcgccat
     3481 cacgatcgtg atgaggaaca tcagctccgg cttcgctccc gcgagcttca cctgggcgaa
     3541 gacggtggat tgcagcagca gggccgtgac caccacggcg gcccacgcga gtccgcgtcg
     3601 cacggagagt cagtctatcc ctctccatat cccccggtcg gcctaccgtt gactgccctc
     3661 gaccacgccg ccggagagga cgaccagcac ctccgacagc cgggacacgt cgacgtacgg
     3721 gcgaacgagg atctgctcct ggaagcccga ctcgttcggc gcgacgtggg agacggcccc
     3781 gatctcgatg cccggcggat agacgccgtc ctggcccgag gtcacgacgg gctcgcccgc
     3841 ctcgacgggg gtcccggggt cgatcagatc gaacctcagg tcccgctccc gctgcccccg
     3901 gaggatcccc gtctcgccgg tcgaggcgag ccgcgcatga accgcgtgat ccacgtcgat
     3961 cagcaggagg accttggacc agcgctcgga gaccgacacc acacgcccca cgaggccctc
     4021 ggcggcgacc acggccatgt cgagctccac cccgtcggcc gtgcctcggt cgatcgtcac
     4081 agaggcctcg aagttcgtgg ggacctcgct gatcacggtg gcgccgatcg tctccacctc
     4141 ctgcgtctcc ttgaactcca ggagcgcggt gagctgcttg agccgccgct cgtactcgag
     4201 gaaccggacc cgcacggcct ggagctccgc cacctcggcc tggagccgct cgttctcggc
     4261 ccggagcgag cccagcgaag cgaggttgct gaagaagctc ccgatcggac ggaagaccgt
     4321 ggcgatgccg tcctggagcg ggctgatgtt ggcgagggag atcgttcccg cggcggccag
     4381 cggtccccgc tggccgccgc ggatgtccag cgtgatgatg acgaggctcg tgacgaccag
     4441 cgccaccacg agcagacggg tgctccgctc gcgcctgtag atcgccatac gctgggagtc
     4501 tagccctgcg ggttgctagc gtcgcgtcga ggaggcgagg acctgcttca gggcctcgaa
     4561 ctcctccaag cacttcccgg accccatcac cacgcacgcc agcgggttct cggccacgtg
     4621 gatcggcatc ccggtctcgt gcttcagccg ctcgtcgagg cccttgagga gggctccgcc
     4681 gcccgtcagc acgatcccct ggtccatgat gtcggcggcc agctcgggcg ggcagcggtc
     4741 gagcgtgttc ttgatcgcgt ccaccacggc gttgaccggc tcctcgaggc ccttgcggat
     4801 ctcctcggcc gagacgtgga tcgtcttggg gagcccggtg acgaggtcac ggcccttgat
     4861 ctcggcgatc atctcgtcgg gcatggggaa ggccgacccg atcgccatct tgatcgcctc
     4921 ggccgtccgc tccccgagaa cgagcgagta ctccttcttc acgtactgga tgatcgcctc
     4981 gtccagctcg tccccgccca cgcggaccga ggtggcggtg acgatcccgc ccagcgagat
     5041 gatggccccc tcggtggtgc cgcccccgat gtccacgacc atgttgccgg tcggctcgtg
     5101 gacgggaagc ccggcgccga tcgcggcggc catcggctcc tcgatgatgt aagcggcgcg
     5161 ggcaccggcc gagatcgtgg cctcctcgac cgcacgctgc tcgaccccgg tgatcccgct
     5221 cgggacgcag accacaacgc gaggcttggc caggaaccgc cgcgtgtgca ccttctggat
     5281 gaagtaccgc agcatcttct cggtgatgtc gaagtcggcg atcacgccgt ccttcagcgg
     5341 gcgcacggcc tggatgtggg acggggtccg gccgatcatc cgcttcgcct cggcccccac
     5401 ggccaggacc tgcttcgtca tcgtgttgat cgcgacgacg gagggctcgt tcaggacgat
     5461 gccacgcccg cggacgtaga cgagggtgtt ggcggtgccg aggtccacgg ccatgtcccg
     5521 accgaggagc ccggtgatgc cctccggccg gcggtcgcga gagagccgct cggtccgctg
     5581 tggctcaccg ttctgcgtca atctcacctt cccggggaag ctcgaggccg ggtggcctct
     5641 gtctacagga ctccatacgg taatgcaacg acgcggccgc gtccaaccgg actcgtttgt
     5701 gcgcaaccgt cgcgaagggt acaacgaaac ggtgcctgag cgctgggccc tcgcgggatg
     5761 tcgccggagg gccggccccg cctccactcc aacctaccga gtagtatgtc tgacctgcga
     5821 aaacctaccg gccgttaagt tgcgcgaccc gggtaaccgt ggcaccctgc gtcgagtctg
     5881 agaagcgggt cgcccaccta tactccgagg gaggtgtcgt gaggatcgcc gtgctgttcc
     5941 cgggccaggg gtcccaatac gccgggatgg ccgacccctg gaccgaccac cccaggggca
     6001 aggagacgct cgccgaggcc tccgacgtcc tcgggtacga cgtcgtggcg gtggcgagcg
     6061 acgaggagaa gctggggctg accgacgtcg tccagccggc gatgttcgcc tgcgacctgg
     6121 ccgcgctccg ggtgctcgag gccgagggtg cgaccttcgc catgaccgcc ggacactccc
     6181 tcggcgagtt cgtggccctg gcggcggcgg gcgccgtgcc ggtggaccag gcgctcaccg
     6241 cggtggtcga gcggggacgc gccatgcagg aggcctccga cacctccccc ggcgggatga
     6301 cggctctggt cggcgtctcc ccacaggagg ccgccgagat cgtggccacc gccggacggg
     6361 gcgacgtcct caccgtggcc aacgagaaca gccccaggca aaccgtcctc tctgggtcga
     6421 tccccgcgat cgaacgggcc gaggagctgg cccgaagccg caaggtccgc acggtgaggc
     6481 tgaaggtcgc gggggcgttc cactcgaagc tgatggagcc cgccctcgcc cgcgtccggg
     6541 cggcgatcgc caagctcgac ttctcggagc cccggttccc cgtggttccg aacgcgagcg
     6601 ggcgccccac ctcgcagccg tccgccctcc gcgacctgct ctcccgccac ctggtgtccc
     6661 cggtgaagtg ggagcgctcg atccgcgcga tggcggacgc cgggatcgac ctcttcgtgg
     6721 aggcggggcc cggcgacgtc ctgtccaagc tggtgaagcg ctccgtcccc ggctcccgcg
     6781 ccgtcccggt gggaagcccc gcggacgcgc gagcgttcgt cgaacaagaa ggagtccgat
     6841 gagcatcacg ttcgagccgt tcgaccactc gccgacgacc ctccgggtgc tcgtcagccc
     6901 tggcgcgggt cgactcaggt acctccctcc gctgcgattc gccggcggtc gcgagctcgt
     6961 ggaggccggc cagccggtgg cccgcgtgct ccagggctcc ggcgaggtca tcctcagggc
     7021 gccggtgggg ggcgtcgtgt cctcgatcct cgggatcgag ggcgagccgc tcgtcgccgg
     7081 ccaggcgatc ctcgccatcg agccggagct cgccccggag ggctcgtgac cgggcggcac
     7141 gcccagatcg ccggattcgg ctccgccctc cccgagcggg tcgtgacgaa cgacgatctc
     7201 gccgcgatgg gcgtggacac ctcggacgag tggatccgca cccgaacggg gatcggggcg
     7261 aggcgattcg tggccgaggg cgagacggcc tcgatgctgg gcgcgcgggc cgcgagggag
     7321 gcgctcgagg cggcgggcgt ggatccgggc gccgtggacc tcacgatctg cgccaccttc
     7381 accggcgacc agcccctccc ctcgaccgcc tcgttcctcc aggcggagct ggggatccac
     7441 ggggcggcct tcgacctcgc cgccgcctgc gcggggttca tctatgccac cgagatggcc
     7501 agggggcaga tcgtcacggg cggggccgag acggtgctcg tcgtcggcgt cgaggtgctc
     7561 tcgccgatcc tcaacctgga ggaccgcacg acctgcgttc tcttcggcga cggcgccggc
     7621 gccgcggtgc tcgtcccggg ggatgagccg gggatcatcg gctcggtcct cgagaacgac
     7681 ggccggcacg cgcacatcct cgaggggccc tcgggcggct cggcctcgcc catctcggcc
     7741 gagaccatcg cgcgtgggga gcacttcatc cggatggccg acgggcgcga ggtgttcaag
     7801 cgggccgtgc gcggcatgtc cgacgccgcg aggcgcctgc tggagaaggc cggcctcgcc
     7861 ctgggcgacg tcacgatgct cgtcccccat caggccaacg cccgcatcat ccgcgccgtg
     7921 gccgagcgcc tctccgtccc gcccgagcgg gtctacatcg acctcgagga ggtggggaac
     7981 acctccgccg cctccgtccc gatcgctctc gaccacgcgt ggcgcgaggg caggctgtcg
     8041 cccggcgacc tggtgctgac ggccgccttc ggggccggcc tggcctgggg agcgaacctg
     8101 atccggtgga ccgcggagcc gccggcgtga gcgggcgcat cgccctggtg accggcgcgg
     8161 ggggcggcct gggccgcgcc gccgcgcttg agctcgcggg ccgcgggatg ccggtggcgt
     8221 gcgcctacgg cacgaacgac gcgggggcca aggagacggc cgagatgatc gaggagcgcg
     8281 gcggcgaggc ccaggccttc ggggccgacg tcgccgacga gcaggaggta cgccgcctct
     8341 tccgcgagat caaggagtgg cggggagccc ccctcgtcct cgtggccaac gccggcgtcc
     8401 gccgcgacgg ccccacggag acgtacccgc tggagcagtg gcagaggacg ctcgacgtca
     8461 acctcacggg ggtgttcctc tgcgtgcggg aggccctccg gggcatgcag ctcgcccgat
     8521 ggggacgggt gatcctcgtc tcctccgccg cgggcctgcg gggcaacccg ggccaggcct
     8581 cctacagcgc ctcgaaggcc ggcgtgctgg gcctgacccg ctcgctctcc cgggaggtgg
     8641 gcgcgcgcgg gatcacggtg aatgccgtgg cgcccggcta cgtcgagacc gcgatcgtgg
     8701 ccgagatgac cgaggcggcc cgcgagcgga tgatggggct gatcgcgacc gggaggccgg
     8761 ggcgccccga ggagatcgcc gccgcgatcg gcttcctcgc cggcgaggaa gcctcctacg
     8821 tgaacggagc ggtgctccct gtcgacgggg gtatgaccgc gtagggttgt cgggaactga
     8881 accaaggagg aagggatgga gaggaaggag atcgaggagc gcgtccgcaa ggtcctggcc
     8941 gagcagctgg cccgggaccc ggacgaggtc acgctggagg ccagcttcga ggacgacctc
     9001 gacgcggact ccctcgacct cgtggaggcc gtgctcgcgc tcgaggagga gttcggcatc
     9061 gagatccccg aggaggagat ggagggggtc aagaccgtga aggaggcggt cgacctcgcc
     9121 gcatccaagc tgggggtttc cgcctaggtg accgagcgga aggtcgttct caccggcatc
     9181 gggcccgtca caccggtcgg aactgggcgc gagccgttct gggacgcgct cgtctcggga
     9241 cgatcgggca tcggggagat caccctcttc gacgcctccg aacatcccgt gcggatcggc
     9301 ggggaggtcg agggcttcga tgccgccgac tggatggacc gcaaggccgc caagcgcacc
     9361 gatcgcgcgg tgcacctcgc cgtggcggcg gccaagctgg cgtgggagga cgcgggatcc
     9421 ccgcagatcg accctcagcg cgccgccgtc gtcgtctcca cgggggtggg tggcctccgc
     9481 tccatggagg agcagatcaa gatcctgaac gagcgggggc ccgggagggt gtcggccttc
     9541 acggtgcccg cgatgatggc caacgcggcc acgggaaccg tggcgatgga gttcgggttc
     9601 acgggaccca acgcgtgcat cacgaccgcc tgcgcggcag gggcccacgg cgtcggggag
     9661 gggtaccgct acatcaagca cggcatggcc gacctggccc tggccggggg cacggaggct
     9721 gggatcaccc cgctgtcgct ggccgcgttc gcccagatgc aagcgctctc caggaacgca
     9781 gatcccgagc gggcgtcccg tcctttcgac gccgaccggg acggcttcgt gatctcggag
     9841 gcggcctgcg tgctcgtcct cgaggaggag gagcgggccc gggcccgggg ggcgacgatc
     9901 tacgcggagg tcgccggata cgggacctcg gccgacgcgt tccacatcac ccagccggag
     9961 ccccaggggg cgggggcggt gatcgcgatg cagatggcgc tcaccgacgc cggggagccg
    10021 gcggaggcgg tcgactacat caacgcccac ggcacctcga caccactcaa cgacgccgcc
    10081 gagacccggg ccatcaagaa ggcgttcggc gactacgcgt acgaggttgc ggtctcgtcc
    10141 acgaagtcga tgacggggca catggtcggg gcggccggcg ccgccgaggc cgccgcggcc
    10201 gcgctggccg tgcgcgacgg cgtgatccct cccacgatca accacgagac gcccgacccc
    10261 gagtgcgacc tcgactacgt gcccaacgag aagcgcgaga cggacgtcag gctggcgctc
    10321 tcgaacgcgt tcgggttcgg gggccagaac gcggtcctcg cgctccgacg gatctgatca
    10381 gggcctgcgg acctgcacct cgccgtccac caggacgaag gtcgcccgat acgtgacgcc
    10441 ctcggcgccc ggggctccca gaggctcgcc gcccagcctg agcctgacgg cgccggcgtt
    10501 cgccacctcg agccggatcc ggcggtcggc ccggaacgtc ttcacgaacc ccggcttccg
    10561 ccgtccgtcc tcggccagct ctccgtcgac ccgcacgcgg agccaggtcc tcgcgtcctt
    10621 cacccggagc cggaggtcga tctcgtcctc ctcgcccgcc ggggcgccgg cctcgacggc
    10681 cgggggcgag gcctccgccg ggaggggcgc gggcgggatc gatcgctcca cgctccgcgc
    10741 ggagaccacc accagcgctc ccatcgcgcc cagcgacgcg atccacagga gcgtggcgat
    10801 cagcttcctg tgcgatcgtc gctcgacggg ccgtgggagc cggatcggct ggccgtcccc
    10861 gatcccgtgg gcggtcgcgt agtcggcgac gaggggctcg ggatccagcc ccaggaagcc
    10921 cgcgtactcc cggaggaagg ccctcgcgta cacgggcgcg cggaaggccg agggcggagc
    10981 gtctgcctcc agggcgcgca agaacctggg cttgatccgt gtgtggagcg ctgcatcctc
    11041 gaccgagacg cctcgacgga cccgggcttc cctcaggcga gccccgatcc gtgccttcgg
    11101 agcaaccgag atggccatgc gaacctctcc atccggtgcc cctcccccgt gagcgcgatt
    11161 ataggaccgc ctcggccgaa cggtgggacc tggtcacccg aagaagaggt cgagctcgcg
    11221 cttcgcgttc tcgggggagt cgctgccgtg gacgaggttc tcggtgatga cctccccgta
    11281 gtcgccgcgg atcgtgcccg ggggcgcgtc cttcgggtcg gtgggcccca tcagctcgcg
    11341 caccgccgcc acggcgtcct cgccctcgat cctcgccacc accaccgggc cggacgtgat
    11401 gaactcgacg agctctccga agaacggctt gtcgcgatgc tcctcgtagt ggcggtcggc
    11461 gagatcctgg tcgatgcgca tcatccgaag gcccgtgatg cggaggccgc gttcctcgaa
    11521 gcggcgcagg atctccccca cgaggcgacg ccggacggcg tcgggcttcg cgatcacgag
    11581 cgtctcctgc gtcgtcatct cagcctttca cgtagcgggg gcgggcatcc gcgacagtgt
    11641 acagagagcc ggtcacgagg atgaggtcgt cggggcccgc ggccttcgtc gccgcgtcga
    11701 gcgcggcggc cacggttggg tgcgcgatcg cggggatccc cgcacgctcg gcgacggccg
    11761 cgagccgctc cgggtccatc gcccgcgggt ggctcgacgc ggcgacgaag gccgacgtcg
    11821 tcctgggcgc gagcgccgag gcgatcccgg tcgcgtcctt cgtctccagc accgccagca
    11881 ccaggtagag acgctcccag atgaaggcct cccccaccgc cgccgccagc gcggcggccc
    11941 catctgggtt gtgccctccg tcgaggacca cgagcgggtg gcgggccacc acctcgaggc
    12001 gccccggcga gctcgcgccg gccagcgcct cacggaccgc gttcgccccg agctcccggt
    12061 cgagggtgag ctcggccgcg gccacggccg cggccgcgct gcgggccgcg tgctcaccgt
    12121 ggagaggcag ggacaggccc ctgtactcgc cgcgcacccc gcggaccgcg aggcgctggc
    12181 ccccgacggc caggtcccgc accgggaccg tgaagtcctc gccctcgtgg agcagcgtgg
    12241 cccccacctc ggcggcgcgg gcctccagga ccttccgggc ctcgggccgc tgctcccgca
    12301 cgaccgcccg cgtcccctcc ttcaggaccc ccgccttctc cgccgcaacc tcctcgacgg
    12361 tgcttcccag ctcgggatgg tcgaggccga tcggcgtgat caccgccacc tccgagcgga
    12421 tcacgttcgt cgcgtcccag gagccgccca tccccacttc gaacaccccg acgtcgacgg
    12481 gcttgtcggc gaaccatagg gcggccaggg cggcgagcgt ctcgaagtac gtgacccgct
    12541 cgccctcccg atccacctcc tcgaggaagt gccggaggtg ggcgtaggtc tcggcgaact
    12601 cctgctcctc gatcgggtcc ccgcagaagg tgaggcgctc ccggactgtg tggaggtgcg
    12661 gggaggtcag cgcgcccgcg gagacgccgt gggcgcagag cagccgctcg acgagacgcg
    12721 tggtcgtcgt cttcccgttc gtgccggcca cgtggaccgt cgggtaggcg agctgcgggt
    12781 cgcccatcag ctcgaccact gcgcggatcc ggtcgagggt ggggatcatc ctgccctcgc
    12841 cccgcgcctc caggtcggcg acggcgtcct cgaagcgcac gtcagccgca gcccagctcg
    12901 tcgagctgct cgcggagcgc tgcctcctcc tccttgagcc gggtcagcct ccgccgctgt
    12961 tcctccacga cctcggccgg ggccttcgtc acgaagccct cgttggccag cttccgctcg
    13021 gccttcccgg cctcctcgcc cagcgcctcg atccgacttc gcaggcgatc gcactcggcc
    13081 tcggggtcga ggaccccctc cagcgggatg aacacctcgg ctccctgcac gtggaggcgc
    13141 gccgagcccg agggagagga agcctccccg gtcgtgacga tggagcccac ccgggccagc
    13201 ctccgcacct cctcggagag cgtgcccagg atctgtcgtt gggcggcgtc gccctccacc
    13261 ctcaccctca gctcgaggga cggcggcacc cggtgatcgc tgcggaagcg ccggaccgcc
    13321 gtgacgagat ccatggcgaa ccccactcga gcctcggcct cctcgtcgcg atgagcgggc
    13381 atcggctcgg gccacgaggc gatcatgatc gacccctcca gaacgaagcg ccgacggatc
    13441 tcctctgtga cgaacggcat gatcggatgg aggaggcgca ggctccgctc gaggacccac
    13501 gccaggaccg cggcggcgtc gcggcgatcc tcctcggatc cctcgtagaa ccgcggcttc
    13561 gccatctcca ggccccagtc gcagtactcg gaccagagga accgatagag ggcctgcgcc
    13621 gcgtccgcga accggtaggt ctccatcgcg gcgtccacct ccgcgtggca cgcctcgacc
    13681 cgggagagca gccaccggtc ggtcacggtg agacgctcgg gcgacggcgg cgagagatcg
    13741 tcgccttcga ggttcgtgcg gacgaggcgc gcggcgttcc agagcttgtt ggcgaagtgc
    13801 ttcccggcct cgatctggtc ctcggccagg ggcaggttct gctgctcggg cgaggccatc
    13861 cgcgacagcg cgaagcgaag cgcgtcggcc ccgtaccgct cgatcatatc gaggggatcg
    13921 atcacgttcc ccagcgactt ggacatcttg cggccctggg catctcggac cagcccgtgg
    13981 atcacgacgg tgtggaaggg cagatcgccg gtcagccgca gccccgacat caccatccgg
    14041 gccacccaga ggtagaggat ctcgtagccc gtcaccagga ccgacgtggg atagaagtag
    14101 tggagatcct ccgtctcctc gggccacccc agcgtggaga acggccagag ctgggacgag
    14161 aaccaggtgt cgaggacgtc cgggtcctgc tcgatctcgg cgctcccgca ctgtgcgcac
    14221 cccttcgggt cctcgatcga ggcgaactcg tgcccgttcg agcagtacca gacggggatc
    14281 cggtggcccc accagagctg gcgggagatg ttccagtccc gcaggccctc cagccactcc
    14341 aggtatcgat ccttccagcg gggcggtgag aagacgaggc cccccgactc ggccgcctcc
    14401 atggcggggc ccttcagcgg gtcgaccttc acgaaccact gctcccccga cagccaaggc
    14461 tcgatctcgg accggcatcg atagcagtgg cccacggagt ggacgtacgg cctctcctcc
    14521 cggacgatga ggcccagctc gtccagccgg tcgcggacgg cccgtcgggc ctcgtaccgg
    14581 tccaggcctc ggaaggcctc gggaacggcg tcgttgagcc tggcctcgga cgtgaggacg
    14641 ttccgggcct ccagcccggt ccgctcggcg atctcgaagt cgttggggtc gtgcgctggc
    14701 gtgaccttga cggcgccggt gccgaacgac ggatccaccg tctcgtcggc cacgatcggc
    14761 agctctcgcc catccagggg atgccgcacc gagcgtccca ccaggtcttg gtagcgctcg
    14821 tcgtccgggt ggaccgcgat ccctgtgtcc cccagcatcg tctcgacccg ggtcgtggcc
    14881 acctcgatgt ggccgtcgcc gtcggccatg gggtaccgga aggtcacaag ctctccctcc
    14941 acctcctcgt gctccacctc ggagtcggag agcgccgtct gatccgtggg gcaccagttg
    15001 atgatgcgct cgccccggta gatcagcccg tcctcgtaca ggcggacgaa cgccacgcgc
    15061 accgcgtgct gcagcccctc gtccatcgtg aagcgcagcc ggtcccagtc acacgagctg
    15121 ccgagggcct tcatctgctc gacgatccgg cccccatacc gctcccgcca ctcccacacg
    15181 cgctcgatga aggcctcacg tccgatcgcc tgcttgtcga tgccctcctc cagcaggcgt
    15241 cggccgacca ggacctcggt tccgatcccc gcgtgatccg tgcccggcag ccagagcgcc
    15301 tcgtacccct gcatacggcg acggcggatc agcgcgtcct ggatcgtgtg atcgagcgcg
    15361 tgcccgatgt ggagagcgcc cgtcacgttc gggggcggca gcacgatgga gaagggctcg
    15421 cgctcctcgg tcggctcggc gcggaagtac cctctctcga tccagtccgc gtaaagtcgc
    15481 gactcgacct cggatggctc gtaggcggac gccgtggtca tggccgcgag tgtaccgcga
    15541 gcccttccgg ccgcccgatc agcgaggctt cactcggtcc cccgaacgac cgatagagag
    15601 acggaggctc ccgtgaggga gtcgtcccaa ggaaaggctg aggatggtgc agcgcgaggg
    15661 acccgggccc gggggcaccg ctcccgaagc ccccgcgccg cccgctgctg cgggcggctt
    15721 gatcgtgcga gtcttccagg agacgcccga cat
//
LOCUS       DASUTW010000238         7725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_75597, whole genome shotgun sequence.
ACCESSION   DASUTW010000238 DASUTW010000000
VERSION     DASUTW010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7725
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75597"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1077
                     /locus_tag="VF058_08155"
     CDS             85..1077
                     /locus_tag="VF058_08155"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX6208313.1"
                     /translation="MRWVGASGPARPDGTPMRPDTPYPLASVTKLYIGTAILQLHEQG
                     HVDLDAPITAYLPVDRVARLHRLGGVDHTPRITVRHLLSHTSGLPDFLEDRPSGGRRI
                     YEEIEDGVDRSWTFDDALEIARGMRPRFEPQDLSADRQKARYSDTGFQVLLMIIEAVA
                     GRPFHEVFEERLFRPLGLRHTWVPGRSEPLDPPAHEPAVVWAKGRPLDIPQAMASFND
                     LIGTADDTLRFMWALTRGEVFRDRATYRLMHERWNRIFYPMRYGLATMRYRIPRLFAP
                     GRRTLTLVGHSGATGSWLFHCPELDLVLTGTVDETSARAVPFRFLPRVLKALQS"
     gene            complement(1194..1670)
                     /locus_tag="VF058_08160"
     CDS             complement(1194..1670)
                     /locus_tag="VF058_08160"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX6208314.1"
                     /translation="MTARKDEPESAEQATPVTLQLEEAGYRFRVDFGLPGAPPLLMDE
                     PEPLGEGSGPNAPRLLVAAVVNCLSASLLFCLRKARLEVRGMRASGSAMFTRSEAGRL
                     RVQGIRVRIEPEVVGGESGRLRRCLEIFEDYCVVTQSVRGGIDVDVEVATTSAEAA"
     gene            complement(1865..2500)
                     /locus_tag="VF058_08165"
     CDS             complement(1865..2500)
                     /locus_tag="VF058_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208315.1"
                     /translation="MGGMRSARVGILTVVALTALGGLALAAGLPESLRSSPQDFLESL
                     GLEPEEPGATGGGEDGFDPAPLREGSEASEHASQTAIDVLTVIQAWRNGEFESGCEFG
                     QAVSAAARGAEPEDDFDPCERDGSNGEEASGAGKAKGEEMSAAGKAKGQAAAEAGKAK
                     GAEASAAGKAKRGDAPGGGASNGGGGGGSGPPSNPGPPPGAGPPGGTPGGP"
     gene            2635..4155
                     /locus_tag="VF058_08170"
     CDS             2635..4155
                     /locus_tag="VF058_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006621280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEX6208316.1"
                     /translation="MRRIALLPALTLLISLALPAAPSGGATVLHVTTTDDEIVNDGDC
                     SLREALIAAETDAPRDACPAGSGADVVRLPAGVFVLTIPPDGTPDDGEDGDLDIFDQV
                     TVRGAGARRTILDGNGTDRLLHVGPMSVVSVMDMTISNGWTDDAFGGGAILNVTASLT
                     LRRVTVKGSEATAGDGGGIESTNAGAVLTVVDSSVIDNHTTDFGGNGGGIANGNGSMA
                     NLTRVTVSGNSAFQQGGGIANEAGSTIDLTSATITDNVANADEGGAEGTGGGIQHNGL
                     AATLRSTLVAGNRDLEPNVAAHDPDCSSILPFTSEGHNLIGEPSPGCDFPAASTDLMG
                     EDPLLRSVRDNGGPTDTHALRPGSPTLNRIPRANAACTARDQRGVPRSGRCDIGAYEL
                     VRCRGVIINRVGTGGKDRLRGTAKRDGILGLGGGDTIRGGKGNDAICGGPGRDVLIGG
                     AGRDVLVGEKGPDTLRGGGGRDLLLGGPGGDALRGGAGRDRCRQGKGSGPISGCEQ"
     gene            complement(4136..4975)
                     /locus_tag="VF058_08175"
     CDS             complement(4136..4975)
                     /locus_tag="VF058_08175"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEX6208317.1"
                     /translation="MTVLGPGADGIVIATPDPRSAAMDRLTLRSAGIPAALVEDLGLA
                     LAAEPEGDDPPSEAAAAWAVVTRRPAVAREGDRLAVGRPDGSVEISKPATFSSDLLGG
                     DPPPRRAAVFGGAWIGEGEPEYREAVELGRRAAGAGVHLVCGGYGGIMEAVSRGAAGA
                     GGAAIGISIAAWDGRVTPNPWLTHVVAARDLLARFPLLLDADVWVAFPGGVGTLAEIA
                     LCWNLMQMSIEPRPLLLVGERWGTLVEAFRRELIVTESGHHDLIRVCDVDEAAGALTA
                     RSR"
     gene            complement(4972..6393)
                     /gene="thiI"
                     /locus_tag="VF058_08180"
     CDS             complement(4972..6393)
                     /gene="thiI"
                     /locus_tag="VF058_08180"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HEX6208318.1"
                     /translation="MARLMLKTAGEIGVKSPRTRRRFLRVLRRNVRRALNRGGVQASV
                     QAGWGRIMVRTSDPEAATEVLSRVFGLHSVSLVETVSFDGLDDLVAKAAERSRDRIRG
                     RTFAIRPRRSGEHDFRSHDVAVKLGAALLEDSAGVNLDQPEVEVPVEIVNDEAHLVLE
                     ATKGAGGLPLGTGGRALAMFSGGFDSPVATWMAMSRGTTVDLVVFDLGGCAQTDGALT
                     VAKALADRWAPGLEPVAHVVDLLPVVAALTQRVEGRLRQLVLKRAMYRAATILADERQ
                     VDALVTGESIGQASTQTLRNLSVVERATTYPVLRPLVGMSKEEIMGRAREIGTHEASA
                     RVHEYCSIATGPVETAARFEEVADADGEIEESVLRAGVEKRRVVELATWTPGPLPEYV
                     LEQALEGMTVIDVREEGEGPDVGDRRLPFSRLHEWLPQLDPKEPYLFVCSVGSRSELV
                     AEQLWGQGYRAYCLAGGAHRLRR"
     gene            complement(6409..6864)
                     /locus_tag="VF058_08185"
     CDS             complement(6409..6864)
                     /locus_tag="VF058_08185"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEX6208319.1"
                     /translation="MATPSKPKRPRDELGRPLPWDAPNRLPMEDFDRLPLEENHRLAI
                     AYWNDGNFFPAHEAWESCWKQSKGTEEEELFKGLSQLGAGYVHARRMNHHGAKVLLRR
                     GFSRVVRYLPERRGLDLRAIAGAAREHADEVERAQRAGEPVPQVTPPTI"
     gene            6946..>7725
                     /locus_tag="VF058_08190"
     CDS             6946..>7725
                     /locus_tag="VF058_08190"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007031535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX6208320.1"
                     /translation="MTAALLTDLYELNMVASYLKRDMTAPGTFSLFVRELPPGWGFFV
                     ATGLEACLDELEGLRFTEDDLEYLRRTLDYDEDTLRGLADLRFTGDVVAVPEGRVVYP
                     TEPLLEVTAPLPEAQLVETILLNRVTYQTAVASKAARCVLAAPDKDMVDFALRRVHGG
                     EAGMGIARATAIAGFTATSNVEAARRFGLTASGTMAHSYIEAFESEEEAFRAFAQDFP
                     DQVVLLVDTYDTMRGVQKAIEVVRELPEVHLRAIRLDSGDLG"
BASE COUNT         1112 a         2935 c         2645 g         1033 t
ORIGIN      
        1 cccccccccc cccccccccc ccccctcgcc tcccggaaga agatccccca cgtcatcctg
       61 ggcctggcga gcggggatgg gtcgctgcgc tgggtcgggg cttctggacc cgcccgcccg
      121 gacgggaccc cgatgcggcc cgacaccccg taccctctcg cgagcgtcac gaagctctac
      181 atcgggaccg cgatcctgca gctccacgaa caaggccatg tcgacctcga tgcccccatc
      241 accgcgtacc tgcccgtgga ccgggtcgcc cgcctgcatc ggctcggcgg cgtggaccac
      301 acgcccagga tcaccgtccg gcacctcctc agccacacct ctgggctccc ggacttcctg
      361 gaggaccgtc cgagcggcgg ccgccgcatc tacgaggaga tcgaggacgg cgtggaccgg
      421 tcgtggacct tcgacgacgc cctcgagatc gcacgtggga tgcggccccg cttcgagccg
      481 caggacctct ccgccgatcg ccagaaggcc cgctactcgg acaccggttt ccaggtgctc
      541 ctgatgatca tcgaggccgt agccggtagg cccttccacg aagtgttcga ggagcggctg
      601 ttccgcccgc tcggcctgcg gcacacatgg gtccccggcc gatcggagcc gctcgaccct
      661 cccgcccacg agccagcggt cgtgtgggcc aagggacgtc cactcgacat cccccaggcg
      721 atggccagct tcaacgacct gatcgggacc gccgacgaca ccctgcggtt catgtgggcg
      781 ctgacgcgcg gcgaggtgtt ccgggaccgc gcgacgtacc ggctgatgca cgagcggtgg
      841 aatcggatct tctacccgat gcggtacggg ctcgccacga tgcgctaccg catccccagg
      901 ctcttcgccc ccggccgccg gaccctgacc ctggtggggc actccggagc gacggggtca
      961 tggctgttcc actgccccga gctcgacctc gtgctcacgg ggacggtgga cgagaccagc
     1021 gcccgcgccg tcccgttccg gttcctcccc cgggtgctga aggcgctgca gagctagcgt
     1081 gcgcgtctgg gacggcggcg cgcttggcca cgatccgctt gggccggatg cgtcgtcttc
     1141 ttcaccgatg cggcaccttc acacgagtcg ctgcgactcg cggataggac ggctcaggcg
     1201 gcctccgcgc tcgtggtggc cacctcgacg tctacatcga tcccgccccg cacgctctgc
     1261 gtgaccacgc agtagtcctc gaagatctcc aggcaacgcc ggagcctccc cgactccccg
     1321 cccacgacct ccggctcgat cctgacccgg atcccttgga cccgcagacg ccccgcctcg
     1381 ctccgggtga acatcgcgga ccccgaggcc cgcatgcccc ggacctccag ccgagccttg
     1441 cgaaggcaga acaggaggct cgcggagagg cagttgacca ccgccgccac gaggagccga
     1501 ggggcgttgg gccccgaccc ctccccgagg ggctccggct cgtccatgag caggggcgga
     1561 gccccgggca gcccgaagtc cacccggaac cgatagcccg cctcctcgag ctgaagggtg
     1621 acgggcgtcg cctgctcggc gctctccggc tcgtccttcc tcgcggtcat ggtcgtcctc
     1681 ctctcggggg gcggccccgg ctcccgaacg cacgctcggt gcttccgacc gtagcaggcc
     1741 cctcgagcgg ccggcaggac cctgaggccc gcccgcaggg ggactcccgg cccccgagat
     1801 gggaacgccc ccggcgcgcg ggcgcgccgg gggcgttccg ccccgtgggg cgagagggcg
     1861 ctccttacgg gcctcccggg gtgccgccgg gaggcccggc gcccgggggc ggacctgggt
     1921 tggaaggcgg accggagccg ccgcctccgc ctccgttcga agcgcccccg ccgggggcgt
     1981 ctccacgctt ggccttgccc gcagccgagg cctcggcccc cttggccttc ccggcctcgg
     2041 cggccgcctg gcctttggcc ttccccgcgg ccgacatctc ctcgcccttc gcctttccgg
     2101 ccccggacgc ctcctcgccg ttggagccgt cccgctcgca cgggtcgaag tcgtcctcgg
     2161 gctccgcgcc gcgggcggcc gcggacacgg cctgtccgaa ctcgcatccg gactcgaact
     2221 cgccgttccg ccaagcctgg atcacggtca ggacgtcgat cgccgtctgg ctggcgtgct
     2281 ccgacgcctc gctgccctct cggagcgggg cgggatcgaa gccgtcctct ccgccgccgg
     2341 tcgcacccgg ctcctcgggc tccagaccga ggctctcgag gaagtcctgg ggagagctcc
     2401 gaagcgactc gggcaggcca gccgcgagag cgagaccccc caacgcggtc agcgcgacca
     2461 cggtgaggat ccccacccga gccgatcgca tccctcccat ctccgttcct cctccggctg
     2521 tctctgccga ggtaatcgcc ggagccgccc gtgaggaaac gggttggatc caagccttga
     2581 cggagggggg cccgactgct ttcctctccc cctaaagcgg ccaggagata tcggatgcga
     2641 cggatcgcgc tgcttcccgc cctcacgttg ctgatctcgc tcgcgcttcc cgcggctccg
     2701 tccggggggg ccacggtcct ccacgtcacg acgaccgacg acgagatcgt gaacgacggg
     2761 gactgctctc tgcgtgaggc gctcatcgcc gcggagaccg acgcaccgcg cgacgcgtgc
     2821 cccgcgggca gcggagcaga cgtcgtgcgt ttgccggccg gcgtcttcgt ccttaccatc
     2881 ccccccgacg ggacgcccga tgacggcgag gacggcgacc tcgacatctt cgaccaggtg
     2941 acggtccgcg gcgcaggggc gcgacggacg atcctcgacg ggaatgggac ggaccgcctc
     3001 ctccacgtcg gcccgatgtc cgtagtcagc gtgatggaca tgacgatcag caacgggtgg
     3061 accgacgatg ccttcggtgg aggggcgatc ctgaacgtga ccgcctcgct cacgctgcgg
     3121 cgtgtcaccg tgaagggcag tgaggccacc gccggcgacg ggggcggtat cgagtccacc
     3181 aacgccggcg ccgtcctcac cgtcgtcgac agcagcgtga tcgacaacca caccaccgac
     3241 ttcgggggca acggcggagg catcgccaac gggaacggct cgatggccaa cctcacgcgg
     3301 gtgaccgtga gcgggaactc agccttccag cagggtgggg ggatcgccaa cgaagcggga
     3361 agcacgatcg atctcacctc ggcgacgatc acggacaacg tcgcgaacgc cgacgagggc
     3421 ggagcggaag gcacgggggg cggcatccag cacaacggcc tggcggcgac gctgcggagc
     3481 acgctcgtcg cgggcaacag agacctcgaa cccaacgtcg ccgcccacga ccccgactgc
     3541 tcgagcatcc ttccgttcac atccgagggc cacaacctga tcggcgagcc gtccccagga
     3601 tgtgacttcc ccgccgcgtc gaccgacctg atgggcgagg acccgctgct gcggtcggtg
     3661 cgcgacaacg gcggtcccac cgatacgcat gccctgaggc cgggaagccc gaccctgaac
     3721 aggatccctc gagcgaatgc agcctgtacc gctcgtgacc agcgcggggt tcctcggagc
     3781 ggccgatgcg acatcggcgc ctacgagctc gttcgctgcc gcggcgtgat catcaaccgc
     3841 gtgggcacgg gcgggaagga tcgcctcagg ggaaccgcga agcgcgatgg catcctcggg
     3901 ctgggaggag gcgacacgat ccgcggtggg aagggcaacg acgcgatctg cggcgggccg
     3961 ggaagggacg tcctgatcgg cggcgcgggc agagacgtcc tcgtgggcga gaaggggccc
     4021 gacaccctcc gggggggcgg cggccgggac ctgctgctgg ggggcccggg gggcgacgcg
     4081 ctccggggcg gcgcgggccg ggacagatgc cgccagggga agggcagcgg accgatcagc
     4141 ggctgcgagc agtaagggcc cccgccgcct cgtccacgtc gcacacgcgg atcaagtcgt
     4201 ggtggccgga ctcggtgacg atcagctccc gtcggaaggc ctccacgagc gtcccccacc
     4261 gctcccccac cagcagcagc gggcgcggct cgatcgacat ctgcatgagg ttccagcaga
     4321 gggcgatctc cgccagcgtg cccacgccgc ccgggaacgc cacccacacg tccgcgtcca
     4381 gcagcagggg gaagcgggcc aggaggtcgc gggccgccac gacgtgggtg agccaggggt
     4441 tcggcgtgac gcggccgtcc caggccgcga tcgagatgcc gatcgctgcg ccgccggcgc
     4501 cggcggcccc cctgctgacc gcctccatga tgccgccgta gccgccgcag acgaggtgga
     4561 cgccggcgcc ggcggcgcgc ctccccagct ccaccgcctc ccggtactcg ggctccccct
     4621 ccccgatcca ggccccgccg aacaccgcgg cccgccgggg cggcgggtcc ccgcccagga
     4681 gatcggagga gaacgtcgcc ggcttggaga tctcgacgct gccgtccggc ctccccaccg
     4741 cgaggcggtc gccctcccgc gccacggccg ggcgacgagt gacgacggcc caggccgccg
     4801 cggcctcgga cggcgggtcg tcgccttcgg gctcggcggc gagggccagc ccgaggtcct
     4861 ccacgagagc cgcggggatc ccggcggacc gaagcgtcag gcggtccatc gccgccgacc
     4921 tgggatcggg cgtggcgatc acgatcccgt cggccccggg gccgaggacg gtcatcggcg
     4981 gagccggtgc gctcccccgg ccaggcagta ggcgcggtag ccctgacccc agagctgctc
     5041 ggccacgagc tcggaccgcg accccaccga gcacacgaag aggtagggct ccttcggatc
     5101 gagctggggc agccactcgt gcagccggct gaagggcagg cgccggtcgc cgacgtccgg
     5161 cccctcgccc tcctcccgga cgtcgatcac ggtcatcccc tcgagcgcct gctcgagcac
     5221 gtactcgggc aacggccccg gcgtccaggt tgcgagctcc acgacccggc gcttctccac
     5281 cccggcccgg agcaccgact cctcgatctc cccgtcggcg tcggccacct cctcgaaccg
     5341 agcggccgtc tccaccggcc ccgtcgcgat cgagcagtac tcgtggacgc gggccgaggc
     5401 ctcgtgggtc ccgatctcgc gggcccggcc catgatctcc tccttgctca tccccacgag
     5461 cggacggagc accgggtagg tcgtggcccg ctccacgacc gagagattgc gaagcgtctg
     5521 ggtcgaggcc tgcccgatcg actccccggt gacgagcgcg tccacctgcc gctcgtcggc
     5581 caggatcgtc gccgcccggt acatcgcgcg cttgagcacg agctggcgaa gccggccctc
     5641 gacgcgctgg gtcagcgcgg ctaccacggg aaggagatcc acgacgtgcg ccaccggctc
     5701 gagcccggga gcccaacggt cggccagcgc cttcgccacc gtcagcgctc cgtcggtctg
     5761 ggcgcagccc ccgagatcga acacgacgag gtcgaccgtc gtgccccgac tcatcgccat
     5821 ccacgtggcc acaggggagt cgaagccgcc ggagaacatg gccagggccc ggccgcccgt
     5881 cccgagcgga aggcccccgg cgcccttcgt cgcctcgagc accaggtggg cctcgtcgtt
     5941 gacgatctcc acagggacct cgacctcggg ctggtcgagg ttcacgccgg ccgagtcctc
     6001 gagcagagcg gccccgagct tcacggccac atcgtgagaa cggaagtcgt gctcccccga
     6061 gcggcggggg cggatcgcga acgtcctccc ccgtatccgg tcgcgcgacc gctcggcggc
     6121 cttcgccacg aggtcgtcga ggccgtcgaa cgataccgtc tcgacgagcg agaccgagtg
     6181 gaggccgaag acgcgggaca ggacctcggt ggccgcctcc gggtcggagg tacgaaccat
     6241 gatccggccc cagccggcct gcacgctggc ctggaccccg ccccggttga gggcgcgccg
     6301 gacgttgcgg cgcagcacgc ggaggaaccg ccgccgcgtc ctgggcgact tcacgccgat
     6361 ctccccggcc gtcttcagca tcagtcgagc catcggctcc atggtaggtc agatggtcgg
     6421 aggggtgacc tgggggacgg gctcgccggc ccgctgggcc cgctccacct cgtcggcgtg
     6481 ctcgcgggcg gcgcctgcga tcgcccggag gtccagcccc cgccgctccg ggaggtagcg
     6541 caccacccga gagaacccac gtcgcagcag caccttggcc ccgtggtggt tcatgcggcg
     6601 ggcgtgcacg tagcccgccc cgagctgcga cagccccttg aacagctcct cctcctcggt
     6661 ccccttcgac tgcttccagc acgactccca cgcctcgtgg gcggggaaga agttgccgtc
     6721 gttccagtag gcgatcgcca ggcggtgatt ctcctccagg gggagccggt cgaagtcctc
     6781 catggggagc ctattcgggg cgtcccacgg gaggggtcgg cccagctcgt cgcggggccg
     6841 cttgggcttg gagggcgtgg ccatcggagc gaggataatg ggccctgggg gatgggggat
     6901 gggggatggt ctcgcggatc ccacggactc tctatgctcc agggcatgac cgccgcgctc
     6961 ctcaccgacc tctacgagct caacatggtg gcgagctacc tgaagcgcga catgaccgcc
     7021 ccgggcacgt tcagcctctt cgtccgcgaa ctcccccccg ggtggggctt cttcgtcgcc
     7081 accggcctgg aggcctgcct cgacgagctg gaaggcctcc gcttcacgga ggacgacctc
     7141 gagtacctgc ggcgcaccct cgactacgac gaggacacgc tccgaggcct ggccgacctc
     7201 cgcttcaccg gtgacgtggt cgccgtcccc gaagggcggg tcgtctaccc cacggagccc
     7261 ctgctggagg tgaccgctcc gctccccgag gcccagctcg tcgagacgat cctcctcaac
     7321 cgcgtcacct accagaccgc ggtggcgtcg aaggcggccc gctgcgtcct ggccgccccc
     7381 gacaaggaca tggtcgactt cgccctccgc cgcgtccacg ggggggaggc gggcatgggg
     7441 atcgcccggg ccacggcgat cgccgggttc acggccacca gcaacgtcga ggccgcccgg
     7501 cggttcgggc tgaccgcctc cggaacgatg gcccactcct acatcgaggc cttcgagagc
     7561 gaggaggagg ccttccgggc gttcgcccag gacttccccg accaggtggt gctcctcgtc
     7621 gacacctacg acacgatgcg cggggtgcag aaggcgatcg aggtggtgcg cgagctcccc
     7681 gaggtccacc tccgagcgat ccgcctcgac agcggggacc tcggg
//
LOCUS       DASUTW010000239         5399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_594531, whole genome shotgun sequence.
ACCESSION   DASUTW010000239 DASUTW010000000
VERSION     DASUTW010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594531"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VF058_08195"
     CDS             complement(<1..646)
                     /locus_tag="VF058_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6208321.1"
                     /translation="MSQVATEPIDDSGLGPGPGGEPAQQIVGRSPWELFWRRFRQDRL
                     ATAGIIIIAVITALALAAPLFSNIVGHAPNQVFAAEYPAGALDEFGLPIGPNRDFWFG
                     ADTAGRDLFVRVLYGAQTSLFVALLATFISLVIGVIGGLVAGYYRGKVDTFVSRLIDI
                     VLAMPVLLLALGLVAACGSQQGCLGGVIKPGRLLVAYVIGLFSWPYIARIVRGQV"
     gene            complement(776..2035)
                     /gene="apgM"
                     /locus_tag="VF058_08200"
     CDS             complement(776..2035)
                     /gene="apgM"
                     /locus_tag="VF058_08200"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX6208322.1"
                     /translation="MKKLFYVCLDGLGDEPLPALGDRTPLEAASTPLLDDLAARGRQG
                     LVHTVGPDVAPESDIAVFAILGYDPREEHPGRGVLEAIGSGTAFEDGDLAYRVNFATA
                     EDGRIADRRVGRDLSSEEAVALADEVNREVELPGASAELRATVEHRGVLVIRASEGRL
                     SAQVSNTDPAYAKVGPLGVALETFGDRPLPAEPLDDTEEARRSAGLTNAWSERAAAVL
                     EASEVNARRRADGRLPANLVLARDAGDHVPRLVPIKERFGPSWGCFVEMPVERGIAMV
                     LGMGEVPAPTPEDLAERYAAWGQLALEAIEGYDALYIHIKGPDVPAHDGRAGDKRDVI
                     ETIDASFFGTLLPGLDPGGTIVAVTADHSTSCLRKAHTADPVPLIVAGGGVDPDGTTT
                     FGERAAADGSIGTISGVQIVPTLVDLL"
     gene            complement(2032..2802)
                     /gene="tpiA"
                     /locus_tag="VF058_08205"
     CDS             complement(2032..2802)
                     /gene="tpiA"
                     /locus_tag="VF058_08205"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX6208323.1"
                     /translation="MADRRPIIAANWKMHKTHLEAIETVQKLSYLLEVADTERVEVVI
                     CPPFTALRSVQTVISSDRLPFRIGAQDVHWEEKGALTGEVSPPMLAALDCAYVIVGHS
                     ERREHFGETDADVARKARAALAAGIVPIVCVGETLEERDAGRTEEKVTRQVREGLAKV
                     PPDRAGELVLAYEPIWAIGTGRAATPQDAGEVIAAIRATLASAFSDEASRAVRIQYGG
                     SVKAGNIREFMAHPEVDGALVGGASLDPEELALIVRYR"
     gene            complement(2795..3991)
                     /locus_tag="VF058_08210"
     CDS             complement(2795..3991)
                     /locus_tag="VF058_08210"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX6208324.1"
                     /translation="MGRLRGIEDLDDPSGRRVFVRADFNVPLEGGEVGDDLRIRATLP
                     TIEELRGRGAALVLASHLGRPKGEPRADLRMDPVATRLEELAGVPVAKVDDVTGPEAE
                     QAAAGLAPGELLLLENLRFDPGEEANDPSFARRLAGMADAYVNDAFGAAHRAHASVVG
                     IAENLPSVAGRLMAREIEVLSRLLEGPERPFVAILGGAKVSDKLGVVRSLMERVDALL
                     IGGAMAFTFILAQGGEVGSSMAEAERVDEVRETMEEASRRGVLIVLPEDVVAAAEPAQ
                     DARKQTVRAGEVPTGLMGLDIGPRSVEEFARVLADARTIFWNGPMGVVELEPFSLGTR
                     GVAQAVAGADAFSVVGGGDSIAAIRRLGFEDDVDHLSTGGGASLEFLEGRELPGVAPL
                     MEEDDG"
     gene            complement(3994..5001)
                     /gene="gap"
                     /locus_tag="VF058_08215"
     CDS             complement(3994..5001)
                     /gene="gap"
                     /locus_tag="VF058_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013154027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX6208325.1"
                     /translation="MSIKVGINGFGRIGRNFFRAAKQRGLDLDFVGVNDITDAGTLAH
                     LLKYDSVLGTLDADIQASEGGITVDGDEIRVLAERDPGALPWKDLGAEVVVESTGLFT
                     KREDAEKHLSAGAQKVVISAPAKGEDITIVLGVNHEAYDPSSHHVISNASCTTNCVVP
                     MAKVLADAFGIDRALMTTIHAYTNDQNILDLPHKDLRRARAAAINIIPTSTGAAKATS
                     LAIPELKGKMDGVAMRVPVPDGSLTDLVCVLEQEPSADEVNGAFAAAASSGPLRGILR
                     YSEDPIVSSDIVGDPHSCIFDAPSTMAIGNLVKVLGWYDNEWGYSNRLVDLVEMVGSS
                     L"
     gene            complement(5070..>5399)
                     /locus_tag="VF058_08220"
     CDS             complement(5070..>5399)
                     /locus_tag="VF058_08220"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX6208326.1"
                     /translation="PRDADLVLDCRFLPNPHWIEELRPLPGTDEAVRSYVISQHAYGE
                     FMKRLRSLLDFVLPGYVAEEKSYLTIAIGCTGGRHRSVVVAEEVAGHLESQGRRVALD
                     HRDLERE"
BASE COUNT          771 a         2007 c         1858 g          763 t
ORIGIN      
        1 ggacctggcc ccggacgatg cgggcgatgt agggccagct gaagaggccg atcacgtagg
       61 ccaccagcag ccgccccggt ttgatcacgc cccccaggca cccctgctgg ctgccgcacg
      121 ccgccacgag ccccagggcc aggagcagga cgggcatggc cagcacgatg tcgatcagcc
      181 gggagacgaa ggtgtccacc ttcccccggt agtagccggc caccagcccc ccgatgaccc
      241 cgatcacgag cgagatgaac gtggccagga gggccacgaa gagcgacgtc tgcgccccgt
      301 agagcaccct cacgaacagg tcgcggccag cggtgtcggc cccgaaccag aagtccctgt
      361 tgggcccgat cggcaagccg aattcgtcca gggcccccgc cggatactcg gccgcgaaga
      421 cctggttcgg cgcgtgcccc acgatgttgg agaagagcgg cgcggccagg gccagggccg
      481 tgatgacggc gatgatgatg atgccggccg tggcgagccg atcctggcgg aagcggcgcc
      541 agaagagctc ccacgggctc cgtccgacga tctgctgcgc gggctctccg cccgggcctg
      601 gaccgaggcc agaatcgtcg atcggttcgg tcgcgacctg cgacacgcgc cccccttcca
      661 ggagaacctg ccggatacta cccggcccga gccctctgag caaaggtcac gaggctgcac
      721 cgggcacagt gcatctcggg gaggtatacg cgctcccctc gggggaggat tggggtcaga
      781 ggagatcgac gagggtgggg acgatctgga ccccggagat cgtcccgatc gagccgtccg
      841 ccgccgcccg ctcgccgaag gtggtcgtgc cgtccgggtc caccccgccg ccggccacga
      901 tcagcggcac cggatcggcc gtgtgggcct tccggaggca cgaggtggag tgatcggcgg
      961 tcacggccac gatcgtcccg cccgggtcga gtcccgggag gagggtgccg aagaacgagg
     1021 cgtcgatcgt ctcgatcacg tctcgcttgt ccccggcacg accgtcgtgc gccggcacgt
     1081 ccggcccctt gatgtggatg tacagcgcgt cgtacccctc gatcgcttcc agcgccagct
     1141 gtccccacgc ggcgtaccgc tcggcgaggt cctcgggggt gggcgccggc acctctccca
     1201 tccccaacac catggcgatg ccccgctcga ccggcatctc cacgaagcat ccccatgacg
     1261 gcccgaatcg ctccttgatc gggacgagcc gagggacgtg atcgccggcg tcccgggcca
     1321 ggacgaggtt ggcgggcagg cgcccgtcgg cccggcggcg ggcgttcacc tcggaggcct
     1381 ccaggaccgc cgcggcccgc tcgctccagg cgttcgtgag gccggcgctc cggcgggcct
     1441 cctcggtgtc gtcgaggggc tcggcgggca gcggacgatc cccgaacgtc tccagggcca
     1501 ccccgagcgg gccgaccttc gcgtaggcgg gatccgtgtt cgacacctgc gccgagagcc
     1561 ggccctccga ggcccggatc acgaggaccc ctcgatgctc cacggtcgcc cggagctcgg
     1621 ccgaggcgcc ggggagctcg acctctcgat tgacctcgtc cgcgagcgcg accgcctcct
     1681 cggaggagag gtcgcgaccc acgcgccggt ccgcgatgcg gccgtcctcg gcggtggcga
     1741 agttgacccg atacgccagg tcgccgtcct cgaaggccgt ccccgagccg atggcctcga
     1801 ggacgccccg ccccgggtgc tcctcgcggg ggtcgtagcc gaggatggcg aagaccgcga
     1861 tgtcggactc cggggccacg tcggggccga ccgtgtggac gagcccctgc cggccccggg
     1921 cggccaggtc gtcgagcaga ggggtggagg cggcctccag gggggtgcgg tccccgaggg
     1981 ccggcagggg ctcgtccccc aggccgtcga ggcacacgta gaacagcttc ttcatcggta
     2041 cctcacgatc agcgcgagct cctcggggtc gaggctggcc ccgcccacga gggcgccgtc
     2101 gacctcgggg tgggccatga actcgcggat gttccccgcc ttcacgctgc ccccgtactg
     2161 gatccggacc gcccgggacg cctcgtcgga gaaggccgag gcaagcgtgg cgcggatcgc
     2221 ggcgatcacc tcgcccgcgt cctggggggt cgcggcccgg cccgtcccga tggcccagat
     2281 cggctcgtag gcgagcacga gctcgccggc ccggtcgggc gggaccttgg ccagcccctc
     2341 gcggacctgc ctcgtcacct tctcctcggt cctccccgca tcccgctcct cgagcgtctc
     2401 gcccacgcac acgatgggca cgatccccgc cgcgagcgca gcccgcgcct tgcgggccac
     2461 gtcggcatcg gtctcgccga agtgctccct gcgctcggag tggcccacga tcacgtaggc
     2521 acagtccagc gcggccagca tcgggggcga gacctcgccc gtcagcgcgc ccttctcctc
     2581 ccagtggacg tcctgggcgc cgatccggaa cgggaggcgg tcgctggaga tcaccgtctg
     2641 gacgctgcgg agggctgtga agggcgggca gatcacgacc tcgacccgct cggtgtcggc
     2701 cacctccagc aggtacgaga gcttctgcac ggtctcgatc gcctccaggt gcgtcttgtg
     2761 catcttccag ttcgccgcga tgatcgggcg tcggtcagcc atcgtcctcc tccatcaggg
     2821 gggccacccc ggggagctcc cgcccctcca ggaactcgag cgacgctccg ccgccggtgg
     2881 acaggtggtc cacgtcgtcc tcgaacccga gccgccggat cgccgcgatg ctgtcgcccc
     2941 cgcccacgac cgagaaggcg tcggccccgg ccaccgcctg ggccactccg cgggtcccga
     3001 gcgagaacgg ctccagctcc accacgccca tcggcccatt ccagaagatc gtccgggcgt
     3061 cggccagaac gcgggcgaac tcctcgaccg agcgggggcc gatgtcgagc cccatgagcc
     3121 cggtcgggac ctcgccggcg cgcaccgtct gcttccgcgc gtcttgggcc ggctcggccg
     3181 cggccacgac gtcctcggga aggacgatca gcaccccccg ccgggacgcc tcctccatcg
     3241 tctcgcggac ctcgtcgact cgttcggcct cggccatgga cgagcccacc tccccgccct
     3301 gggccaggat gaaagtgaag gccatggcgc ccccgatcag gagcgcgtcc acccgctcca
     3361 tgagcgagcg cacgaccccg agcttgtccg agaccttcgc ccctcccagg atcgccacga
     3421 agggccgctc cgggccctcc aggaggcggg acagcacctc gatctcacgg gccatgaggc
     3481 ggccggccac cgaggggagg ttctcggcga tccccacgac cgaggcgtgc gccctgtggg
     3541 ccgccccgaa cgcgtcgttc acgtacgcgt cggccatccc ggcgagccgg cgggcgaagc
     3601 tcgggtcgtt cgcctcctcg cccgggtcga agcgaaggtt ctcgaggagc agtagctccc
     3661 cgggagcgag cccggcggcg gcctgctccg cctccggccc cgtcacgtcg tcgaccttgg
     3721 ccacggggac gcccgccagc tcctcgagac gcgtggccac cgggtccatc cggaggtcgg
     3781 cccgcggctc gcccttcggc ctccccaggt ggctggcgag cacgagcgcc gcgcccctcc
     3841 cccgaagctc ctcgatcgtg gggagcgtgg cccggatacg caggtcgtcg ccgacctcgc
     3901 cgccctccag cggcacgttg aagtccgccc gcacgaacac ccggcgcccc gacgggtcgt
     3961 cgaggtcctc gatcccgcgc agcctcccca ccgtcagagc gaggagccga ccatctcgac
     4021 gagatccacg agccggttcg agtagcccca ctcgttgtcg taccagccga ggaccttcac
     4081 caggttcccg atcgccatcg tggacggcgc gtcgaagatg cacgagtggg gatcgcccac
     4141 gatgtcgctg gagacgatcg gatcctcgct gtagcggagg atcccccgca ggggaccgga
     4201 gctcgcggcg gccgcgaacg ccccgttcac ctcgtccgcc gagggctcct gctccagcac
     4261 gcagacgagg tcggtcaggg atccgtcggg gaccggcacg cgcatcgcca cgccgtccat
     4321 cttccccttc agctcgggga tggccaggga cgtggccttc gcagcgcccg tggacgtcgg
     4381 gatgatgttg atggcggccg cgcgcgcccg gcgcagatcc ttgtgcggca gatcgaggat
     4441 gttctggtcg ttcgtgtagg cgtggatcgt cgtcatcagc gcgcggtcga tcccgaaggc
     4501 gtccgccagg accttcgcca tggggacgac gcagttcgtg gtgcacgacg cgttggagat
     4561 gacgtggtgg ctcgacgggt cgtaggcctc gtggttcacg cccaggacga tcgtgatgtc
     4621 ctcgcccttg gcgggggcgc tgatgacgac cttctgagcc ccggccgaga ggtgcttctc
     4681 cgcgtcctcg cgcttcgtga agagcccggt ggactccacg acgacctccg cccccaggtc
     4741 cttccagggg agggcccccg gatcgcgctc ggcgaggacc cggatctcgt caccgtccac
     4801 ggtgatgccc ccctcgctcg cctggatgtc ggcatcgagg gtccccagca ccgagtcgta
     4861 cttcagcagg tgggcgaggg tgccggcatc ggtgatgtcg ttcacgccga cgaagtcgag
     4921 atcgagtccc cgctgcttcg ccgcccggaa gaagttgcgg ccgatcctgc cgaagccgtt
     4981 gatgcctacc ttgatgctca tgcgtctccg cctcctgttc cgggtgggtc acatcctagt
     5041 acccggcgac ggacgacccg aagcgaggct cactcccgct ccaggtcccg gtggtcgagg
     5101 gccacccggc gcccctggga ctcgaggtgg ccggcgacct cctcggccac gaccacggac
     5161 cggtgccgcc cgcccgtgca gccgatggcg atcgtcagat aggacttctc ctccgccacg
     5221 tagccgggca ggacgaagtc gagcagggag cgcagccgct tcatgaactc cccgtacgcg
     5281 tgctggctga tcacgtagga gcggaccgcc tcgtccgtcc ccggcagggg ccgcagctcc
     5341 tcgatccagt gagggttggg caggaaccgg cagtcgagga cgaggtccgc gtctcgggg
//
LOCUS       DASUTW010000240         4867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_465578, whole genome shotgun sequence.
ACCESSION   DASUTW010000240 DASUTW010000000
VERSION     DASUTW010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465578"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="VF058_08225"
     CDS             complement(<1..1302)
                     /locus_tag="VF058_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX6208327.1"
                     /translation="MTRPPVGAAGLDPAEARARLERDGPNLLPAPAGPALWRELLRQL
                     VHFFALMLWVAGGLAFLAGLPQLGIAIFAVVLVNAGFAFAQEFRAERAAQRLRDLLPR
                     RVIVIRGGVRREIDAAELVRGDIVLLAAGDRISADLRVTEAHGMWVDTSTLTGESVPS
                     PVEAGAAVYAGTFLAEGEGAASVVEVGDATRLAGIARLAQAGERPTPPLALELRRLVR
                     TISAIAIGVGVAFFGVATLVGTAPAEGIVLAIGITVALVPEALLPTVTLSLAIGAQRM
                     AARRALVRRLESVETLGSTTFICTDKTGTLTMNEMSAVEVWTPAGWAMIRGHGYEPWG
                     EIEVGGARVGESLREAALAAVRCSTGRVVEREGRWSPLGDPTEVALDVLARRVAGRVV
                     DETEPERARFPFDPRRRRMSLVVGDRVLVKGAPDAVLPLCRT"
     gene            complement(1337..1690)
                     /locus_tag="VF058_08230"
     CDS             complement(1337..1690)
                     /locus_tag="VF058_08230"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6208328.1"
                     /translation="METKAEELIVDAAEVQRRPWQPLGEEVGVRYRALWVGAGSGSYA
                     GVMRMDRGTTIRRHAHAYAVHHMWVLEGECAIGERTLEPGAYAFVPAGVEHGIDSAGP
                     EGCTFFYLYLRAALD"
     gene            1830..2417
                     /locus_tag="VF058_08235"
     CDS             1830..2417
                     /locus_tag="VF058_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6208329.1"
                     /translation="MKARDIMSRPAITVTPETPLKRVAELMVDHRIALVPVVDASGGL
                     LGVISEADLLRGNATPERGEIITSAAREGAVAGTAATVMTMDVVAAEETADVADLFPL
                     MVERHLKGVPVVSDGIVTGVLARRDLLRLLVRTDTDVHDDVMEVLDHVGEEPFSVDVA
                     EGVVTINRWLGPSARRRIEALVRAVPGVMAVVFKE"
     gene            complement(2569..2985)
                     /locus_tag="VF058_08240"
     CDS             complement(2569..2985)
                     /locus_tag="VF058_08240"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEX6208330.1"
                     /translation="MTFDVLSEVNYLAAVIAALAYFAIGALWYSPMLFSKPWQRAIGL
                     VPQEGQSPSPMLFVVTGIAYFVQAVTLGAIARSTGATDLVDGLVLGIFVSMGIIAMGL
                     WVNVSYEQRATSLLWINAGNALVGIVVMSIIMTVWD"
     gene            complement(3348..3650)
                     /locus_tag="VF058_08245"
     CDS             complement(3348..3650)
                     /locus_tag="VF058_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX6208331.1"
                     /translation="MIEGEELDEAQRASLSTPSAVEDHPADVATETFERAKDLSIVEQ
                     LEDRLADVDRALERIEDGSYGVCEACGKPIVRERLAAIPAARFCVEDQARLEASGG"
     gene            3814..4536
                     /locus_tag="VF058_08250"
     CDS             3814..4536
                     /locus_tag="VF058_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX6208332.1"
                     /translation="MSDPGMLDFDWRGRTGPFRIRLAPRVFPPSRTSMLVAETLEIEP
                     GETVIDVGCGSGVLSFVAARLGAARVYGTDVVREAVEVATANARALGLEDRTEFREGS
                     LLDPVEDVVADVVIGDVSGIPDSVAELTGWFPGGHAGGPTGAEVPVALIESVADHLRP
                     GGRMYLPTGTIQDEPRVIEAARRIFGAANMESLAEREFPLPSLVAGSKAVARLMADGL
                     LALRRRGSRLLWRLAIWRCVRR"
     gene            4619..4837
                     /locus_tag="VF058_08255"
     CDS             4619..4837
                     /locus_tag="VF058_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208333.1"
                     /translation="MSLFLFAVGAVLTWAIEVEAQGIDLNLVGVILMIVGAVGFVLSM
                     LFWSPWAPYGRRDTTPPPRDPHGADHVH"
BASE COUNT          704 a         1781 c         1702 g          680 t
ORIGIN      
        1 ggtcctgcac aagggcagga ccgcgtccgg ggcccccttc accagcacgc gatcgccgac
       61 caccagcgac atccgacggc gccgcggatc gaaggggaac ctcgcgcgct cgggctccgt
      121 ctcgtccacg acccgtcccg ccacccgccg ggccaggacg tcgagggcca cctcggtcgg
      181 atcgccgagc ggtgaccacc ggccctcccg ctccaccact cgacccgtcg agcatcggac
      241 ggccgccagg gccgcctctc tgagcgactc gccgacgcgg gctccaccga cctcgatctc
      301 cccccagggc tcgtagccgt ggccgcggat catggcccat ccggccgggg tccagacctc
      361 gaccgccgac atctcgttca tcgtcagcgt gcccgtcttg tcggtgcaga tgaaggtcgt
      421 ggagcccagg gtctccacgg actcgagacg acggaccagg gccctcctgg ccgccatgcg
      481 ctgggcgccg atcgcgagcg acagggtgac cgtgggcaag agcgcctcgg gcacgagcgc
      541 caccgtgatc ccgatggcga gcacgatccc ctcggcgggc gccgtgccca ccagggtggc
      601 cacgccgaag aaggcgaccc cgacgccgat cgcgatggcc gagatcgtcc tcaccaggcg
      661 gcggagctcc agcgccaggg gcggcgtggg gcgctccccg gcctgggcca gtcgggcgat
      721 ccccgcgagc cgcgtcgcgt cccccacctc gaccacggag gccgcgccct cgccttccgc
      781 gaggaaggtc cccgcgtaga ccgcagcccc cgcctcgacc ggcgacggga cgctctcgcc
      841 ggtcagcgtc gaggtgtcca cccacatgcc gtgggcctcc gtgacgcgga ggtcggccga
      901 gatccggtcg ccggccgcga gcaggacgat gtccccccgc acgagctcgg ccgcgtcgat
      961 ctccctccgc acgccacccc ggatcacgat gacccggcgg gggaggagat cgcggagccg
     1021 ctgcgcggcc cgctcagccc ggaactcctg ggcgaaggcg aagccggcgt tcaccaggac
     1081 cacggcgaag atggcgatcc ccagctgggg gagcccggcc aggaaggcca ggcctcccgc
     1141 gacccacagc atgagcgcga agaagtggac gagctgacgc aggagctccc gccacagggc
     1201 cggcccggcg ggtgccggca ggaggttggg gccgtcgcgc tccaggcggg cccgggcctc
     1261 cgcggggtcg agccccgccg cgccgaccgg ggggcgcgtc atggatcgga cggcccccgc
     1321 cacggcccgc tctccctcag tccagggcgg cccggaggta caggtagaag aacgtgcacc
     1381 cctccgggcc cgccgagtcg atgccgtgct cgacgccggc cgggacgaag gcgtaggctc
     1441 ccggctccag cgtccgctcc ccgatggcgc actcgccctc cagcacccac atgtgatgga
     1501 cggcgtacgc gtgcgcgtga cggcggatcg tcgtcccccg gtccatccgc atcacgcccg
     1561 catacgaacc ggagcccgct ccgacccaga gggctcggta gcgcacgccg acctcctcgc
     1621 ccaacggctg ccacggacgg cgctgtacct cggcggcgtc gacgatcagc tcctcggcct
     1681 tcgtctccat ggctcctcct ctgcctccac attgcgtgcg cggagagcga gggggcaggg
     1741 ctcatggtcc cttccgacgt gcgaccttcg gcccatgccc gctccgggcg tcggcgggag
     1801 agtgagatcg acggaaggag cgtggtcgca tgaaagcacg cgacatcatg tccaggcccg
     1861 ccatcaccgt gacgcccgag accccgctga agcgcgtcgc cgagctcatg gtcgatcacc
     1921 gcatcgccct cgtacccgtg gtggacgcct cgggaggcct cctcggcgtc atctctgagg
     1981 ccgatctgct ccgggggaac gcgacgccgg agcgcggcga gatcatcacc agcgccgctc
     2041 gtgagggcgc cgtggcgggg acggcggcga cggtcatgac gatggacgtc gtggccgcgg
     2101 aggagaccgc ggacgtcgcg gacctcttcc cgctgatggt cgagcgacac ctgaaggggg
     2161 tccccgtcgt gtccgacggg atcgtcacgg gcgtcctggc gcgcagggac ctgctcaggc
     2221 tcctggtgcg gacggacaca gacgtgcacg acgacgtgat ggaggtgctc gaccacgtgg
     2281 gcgaggagcc cttcagcgtg gacgtggccg agggcgtcgt gacgatcaac cggtggctgg
     2341 ggccctccgc gcgtcgccgg atcgaggcgc tcgtgcgcgc ggtcccgggc gtgatggccg
     2401 tcgtcttcaa ggaatagcgc gcgtgaccta cgggctccgg gaggtcgagg gctcgcccgg
     2461 cgtggcggac gccctcgccc cgtcctgggg aggcccgtcc tggggaggcc cgtccccggg
     2521 taggagcccg ggacgaggcc ggcgggccgg agctgccggt ccgccggctc agtcccagac
     2581 cgtcatgatg atcgacatca cgacgatccc caccagcgcg ttgccggcgt tgatccacag
     2641 cagcgaggtc gctcgctgct cgtaggagac gttgacccag agccccatcg cgatgatgcc
     2701 catcgacacg aagatcccca gcacgagccc gtccaccagg tccgtggccc cggtggaccg
     2761 ggcgatcgcc cccagcgtca ccgcctgcac gaagtaggcg atccccgtga cgacgaacag
     2821 catggggctg ggggactgcc cttcctgggg caccagccca atcgcgcgct gccagggctt
     2881 cgagaacagc atgggcgagt accagagcgc cccgatcgcg aagtaggcga gcgcggcgat
     2941 cacggcggcg aggtagttga cctcgctgag cacgtcgaac gtcaaggtgt cccccccttg
     3001 tttcgcttgc cgctcagtta tagacgggga cgtgcgaaag cgccagcggg gagcacggcg
     3061 ccctccctcc cgcgcgctgg tgggtcacat ctgcacggcc gcccctcctg tcgggttggg
     3121 cggtcgatct ccggagacgg ccacgatacc cgcggaacgg tcatacggtg agatcgggat
     3181 gggcacgagg gttgcgcggg cggcccgagg gaaaggtacg tccccgagga ggtgagcgca
     3241 tgttcatccc gatcgggatc gggacgctga tcgtcatcat cgtcctgatc gccctgctga
     3301 cctgaaccac cggagggcgg cgacccaggg tcgccgcctc ttccgtctca gcccccagat
     3361 gcctccagcc tggcctggtc ttccacgcag aaccgggcgg cagggatcgc cgcgagccgc
     3421 tcacggacga tcggcttccc gcacgcctcg cacaccccgt aggagccgtc ctcgatgcgc
     3481 tccagcgcgc ggtccacgtc ggcgagacga tcctcaagct gctcgacgat cgagagatcc
     3541 ttcgctcgct cgaacgtctc cgtggccacg tccgccgggt ggtcttccac cgccgacggc
     3601 gtggagagcg aggcgcgttg ggcctcgtcg agctcctctc cctcgatcat cgccccccgg
     3661 agctcctcga gctcccggcg atcctacagc agccgcttgc gtgcctcgtc ccggttcatc
     3721 tcatcacctc gcgtctatcg taacggtcct tccggtcacc gtccccaggt gggggtggcg
     3781 cggagccccg atcgctggga ggatggtcct cacatgagtg atccggggat gctcgacttc
     3841 gactggcggg gcaggaccgg tccgttcagg atccgcctcg cccccagggt cttcccaccc
     3901 agccgcacga gcatgctggt cgccgagacg ctcgagatcg agcccggcga gaccgtgatc
     3961 gacgtggggt gcggctccgg ggtgctcagc ttcgtcgccg cccgcctggg cgccgcccgc
     4021 gtgtacggga cggacgtggt ccgcgaggcc gtggaggtgg cgaccgccaa cgcgcgtgca
     4081 ctcggcctcg aggaccgtac cgagttccgc gagggcagcc tgctggatcc ggtggaggac
     4141 gtcgtggccg acgtcgtgat cggagatgtc tcggggatcc ccgactcggt cgccgagctc
     4201 acggggtggt tcccgggcgg gcacgccgga ggccccaccg gcgccgaggt ccccgtggcc
     4261 ctgatcgagt ccgtcgccga tcacctgcgt cccggcggac gcatgtacct cccgacgggc
     4321 acgatccagg acgagccccg cgtgatcgag gccgcccgca ggatcttcgg ggccgcgaac
     4381 atggagtcgc tggccgagcg ggagttcccc ctcccgtccc tcgtggccgg ttcgaaggcg
     4441 gtggcccgcc tgatggccga cggcctcctg gcgctccggc gccgcggaag caggcttctc
     4501 tggcgcctcg ccatctggcg ctgcgtccgt cgctgacgcc gggtttccca cgccccctcc
     4561 cagggaatcc aacggagcga acaaccaagc gggaggtgac gcagatatgg gtatcggtgt
     4621 gagcctgttc ctgttcgccg tcggcgccgt gctgacctgg gcgatcgagg tggaggcgca
     4681 ggggatcgac ctcaacctcg tgggtgtgat cctgatgatc gtgggagccg tcggcttcgt
     4741 gctctcgatg ctcttctgga gcccgtgggc gccctacggg cggcgcgaca cgactccacc
     4801 gccgcgcgat ccgcacggcg cggaccatgt ccactgaggg actggagacg cgaagaagcc
     4861 ccggccg
//
LOCUS       DASUTW010000241        11350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_725198, whole genome shotgun sequence.
ACCESSION   DASUTW010000241 DASUTW010000000
VERSION     DASUTW010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11350
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725198"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..119
                     /locus_tag="VF058_08260"
     CDS             <1..119
                     /locus_tag="VF058_08260"
                     /inference="COORDINATES: protein motif:HMM:NF014674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208334.1"
                     /translation="RGGKEGVIGFLVGQVMQRSKGAANPQEAQRLLRERLAR"
     gene            225..734
                     /locus_tag="VF058_08265"
     CDS             225..734
                     /locus_tag="VF058_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208335.1"
                     /translation="MSGNARMQRIGLVMLGLLLGAIMVAPAFAHHSPQHTRKQLRALD
                     REVQQFNVRATLGQTKVLLKVGKVTVRGECHDEAGTTETLVVVSGPNGTVVTGDDTSH
                     VIGPATTKDNRTIENPGASATSGVEVSDGYDDQFTTRHRRSVVTGTASSVANDDAGTC
                     LFFGSYIQS"
     gene            976..1263
                     /locus_tag="VF058_08270"
     CDS             976..1263
                     /locus_tag="VF058_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208336.1"
                     /translation="MDDAGTTTAIVFVSGPNGTVVTGSSTSIIIGPGTTKDDRSIENE
                     GASASSGTEVSDGYDDQYTLRYRRIVVTGTASSTANGDSGTCRFFGSYIRS"
     gene            1410..1703
                     /locus_tag="VF058_08275"
     CDS             1410..1703
                     /locus_tag="VF058_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208337.1"
                     /translation="MADDRRRDSVAIGGLESFAGHVAAGLDDRSRDRLNVATLLGFGP
                     RFLHSRGRFHEGGSSSVVWGQVVDERRKVGGEIPGKPYTSGRVNLDTLIKEAS"
     gene            1703..2581
                     /gene="gnd"
                     /locus_tag="VF058_08280"
     CDS             1703..2581
                     /gene="gnd"
                     /locus_tag="VF058_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX6208338.1"
                     /translation="MRIGIVGLGRMGGNMRDRLRGAGHEVLGYERKEGHRDVASYEEL
                     VGALDPPRVAWSMVPAGAATEAVVGELAGLLSEGDLIVDGANSHFRDSMRRAGELADR
                     GIAYVDAGVSGGVWGLEGGYGLMVGGPDDAVASISPLLDALATEGHWVHVGPSGSGHF
                     TKMIHNGIEYGMMQAYAEGFEILHRSEDFPDIDLHAVAEAWRHGTVIRSWLLDLISAA
                     LERDPELSELAAWVDDSGFGRGTVSQAIEEAVPAPAIAAALFARFASRDDSSFAMRLV
                     AALRREFGGHAVQPPD"
     gene            2646..4019
                     /locus_tag="VF058_08285"
     CDS             2646..4019
                     /locus_tag="VF058_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEX6208339.1"
                     /translation="MADEPTQFLLQSDDLPSAWYNIMADVPGEPLPPLHPGTKEPVGP
                     DAFSAIFPMSLIEQEVSAERWIDIPGPVLDVYRLWRPTPMYRARRLEQALDTPARIYF
                     KYEGTSPVGSHKPNTAVAQAYYNKEDGTKLLTTETGAGQWGSALAMAARFFGLECLVF
                     MVKASYEQKPYRRVLMETFGAEVRPSPTSTTEAGKKVLAEHPDSTGSLGIAISEAIEA
                     AVQDETTKYSIGSALNHVLLHQTVIGLETQKQMEMAGEKPDVIIGCVGGGSNYAGFSY
                     PFMADKLAGTLDARFLATEPAACPTLTRGEYRYDFADTGEMTPLVPMYTLGHTFVPAP
                     VHAGGLRYHGDAPSLSALVRAGLMEAKAYTQNPVFEAATQFTAAQGILPGPEPAHAIR
                     AVIDEAIEAREAGEERVILFNLSGHGFFDLQAYDDYLNGRLPEHELDEEDLARGMAEV
                     PDVPVPG"
     gene            4056..5231
                     /locus_tag="VF058_08290"
     CDS             4056..5231
                     /locus_tag="VF058_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020139400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6208340.1"
                     /translation="MAKADLNPSDLLDLEHLLSDEETAVQDTVRGWVGDRVLPEIGDW
                     FEAGEIPSRDLAKELGAMGLLGMHLEGYGTAGASAVMYGLACLELEAGDSGIRSLVSV
                     QGSLAMFPIWKFGSEEQKQEWLPKMAAGEAIGCFGLTEPDFGSNPAGMRASATKDGSD
                     WLLNGTKMWITNGGVADVAIVWARTDDGVRGFIVPTDTPGFTTNDIHKKISLRASVTS
                     ELILENVRLPDDAVLPEVTGMKGPLSCLNEARYGIVWGSMGAARACFESALEYAKTRV
                     QFDRPIGGFQLTQRKLVDMAVELHKGLLLALHLGRMKDEKRARPEHVSFGKLNNVREA
                     IEICREARGILGASGVTLEYPVIRHMNNLESVLTYEGTNEVHTLILGKALTGLDAFE"
     gene            5235..6062
                     /locus_tag="VF058_08295"
     CDS             5235..6062
                     /locus_tag="VF058_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEX6208341.1"
                     /translation="MPPFRGSAGFTERWEHLDVGLEDGVCTVTFSRPDRLNALTFDAY
                     ADLRDLLAELPHREDARVVLITGEGRGFCSGGDVHDIIGALQDMDPRGLLDFTRMTGT
                     VVQRMRECPLPIVAAINGVAAGAGAVIALASDLRIAARSASFRFLFTQVGLAGADMGS
                     AYLLPRLIGTARATELLLLGEGVDAERALEIGLVTQVVEDEELAKAAGDLARRLASGP
                     ALAHETTKMLLTREQDMGLGPSIELEAFAQALLMKSEDHAEFYRAFLEGRDPRWSGR"
     gene            6059..6457
                     /locus_tag="VF058_08300"
     CDS             6059..6457
                     /locus_tag="VF058_08300"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017584560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6208342.1"
                     /translation="MTELFNPSELPKPSGFSHAASASGRMVFVAGQIGEGPDEGLPEG
                     IAEQMDLACANVVTALRAAGAEPERVVWMQIFTTDMAAYREASREIGEAYRKHFGKHF
                     PAMALVEVSGLFEPAALVELTAVAVVPDGG"
     gene            complement(6476..6940)
                     /locus_tag="VF058_08305"
     CDS             complement(6476..6940)
                     /locus_tag="VF058_08305"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX6208343.1"
                     /translation="MAWRSAAMLAALMALTACGGGEPAAVEEGEGEDKTPVSSSEAAR
                     EESLSREVDPDKTQVRITASGIAFDTDRLVAPADQPFQIVLDNQDDLEHNVAVFETIS
                     GVPVFRHPLFRGELIRGPVEIVYDVDALPKGKYVFYCDAHIAEKMKGDFIVR"
     gene            complement(7012..9741)
                     /locus_tag="VF058_08310"
     CDS             complement(7012..9741)
                     /locus_tag="VF058_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX6208344.1"
                     /translation="MRAPFVGRARELEALEAVLRTVEHAEGPAACLIEGDPGTGKSRL
                     LEEALARTDLEHLSVLGYEPERNIPLSSARSLLSRLDLVEHHGPVDLGVFADAPDTLR
                     VCEAAFRAIAGRERVLIAVDDLQWVDDATRGLCHYLLRAAAAEGHTVGLLLATRPSVE
                     ATTFATAVERLLRLIRIQLAGLDREEGIELAAALAPGLDREDAVRTWERARGSPFWLG
                     ALLAAGGTDVRVLLENRLRGLGAQQGRLLSLLALAGLPLEPDEAADALGVEVRRAEEA
                     IDDLVRAGLAVHGQDGVSLAHDLIRTAAVERVPDASRATIHAGLARVFEHRGAGEANL
                     LEALDHRREAGLPSLDLSLRLATSPRRRLLGDDGLARLAAVADQHEPEEAAALMEHLV
                     SLAAELGRHEVALERAAAIADVLGDPRARARMHLAAAEAATELREAGEARRHLDRARA
                     LAEGDASLAIEIDAAEAGALRWAEMRPEEARAMAGRALAGARTAMEGPEALEPRARSA
                     YLRALLAASDDLLATDDPERMLALAEETEAAARGVDEVERFRAIGQRGMALRALGRTR
                     EAETALRLAYYEARRRVFPRMMLEVGWVLVNILHSLGRLEDAQELSEELRGLQARIAV
                     SRPPRVFAITLPHVLELSRGSWRRGMAGLQAEAGAEHDPHMRLHARMAAATAMARLDP
                     SGGAAQVRELLAGAEADAEAAGCGRCRSEVRLVGAEALARIGHLGDARVALEAGRRAL
                     TTDAWLAARAMLAEGVIEASEGRTEALEAFLEEAERQGLVMDILWARLEMAATMVGRG
                     DRGRAAEVLREAGRAAERIGAITEQRLAEQRLRALGVRTWKRGAGAGELTEREREVAG
                     LVAAGASNPEIAASLFLSRKTVERHVSNILAKLGVDNRTQLAARIGGDEGAPR"
     gene            complement(9780..10895)
                     /locus_tag="VF058_08315"
     CDS             complement(9780..10895)
                     /locus_tag="VF058_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6208345.1"
                     /translation="MDAFALTDEQRRLIEEARGIARDRLAEIEGEPGRVNRPLVRALA
                     ERGLLPLMFPEDSEVSAMTLSLLREAVAMESTEAETAMALQGLGAYPILQSGQAEIVE
                     RWIPRVRAGEAVAAFALTEPGGGSDAAALQLRAEPDGDGWRLTGEKAFISNAPDADVY
                     TLFARTTADAGAKGVSAFVVPGDAEGLSGEHVELVAPHAIGHLRLEGVRVGPKDLLGE
                     LDGGFRVAMRTLDLFRPSVGAFAVGMAQRALDLAVAYADERTSMGRPLREHQAVSQQL
                     GEMATRVEAARLLVYAAASAYDRGERVTKASAMAKLFATETAQRVVDAAIQVHGARGL
                     VRGHPLEHLYREVRSPRIYEGTSEIQREVVARELYRD"
     gene            10965..>11350
                     /locus_tag="VF058_08320"
     CDS             10965..>11350
                     /locus_tag="VF058_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208346.1"
                     /translation="MGPRGGTGERRPLVPLDAYGRVLAAVLLAVGWTIASPEAAWSRA
                     VTVALFAGTVFVAFGASGVRGPVAGGVLVALGVAVVAAVLWEAAGEPAVTVVVGLALV
                     LASLGAIVRRLVAHPVVTTSTVVGALT"
BASE COUNT         1606 a         4048 c         4106 g         1590 t
ORIGIN      
        1 tccgcggcgg caaggagggc gtgatcggct tcctcgtcgg ccaggtgatg cagcgctcca
       61 agggcgccgc gaacccccag gaggcccagc gcctcctccg cgagcgccta gcccgatagg
      121 cctattgacg cggcccactc agtcggattt gcttcgcggg cacaagcgag cctgacgggc
      181 gaacgagggg ttccccgcac gctcgccctg cggagggagg tcacatgtcg gggaacgcga
      241 ggatgcagcg gatcgggctc gtaatgctcg ggctgctgct gggagcgatc atggtcgcac
      301 cggcgttcgc gcaccactcg ccccagcaca cgcggaagca gctccgcgcg ctcgacaggg
      361 aggtgcagca gttcaacgta cgggcgacgc tgggacagac caaggtcctt ctgaaggtgg
      421 ggaaggtcac ggtgaggggc gagtgccacg acgaggcggg gaccaccgag accctcgtcg
      481 tcgtctcggg cccgaacggc acggtggtca caggggatga cacgagccat gtcatcgggc
      541 ctgccacgac caaggacaat cggaccatcg agaacccggg cgccagcgcg acgtcgggcg
      601 tcgaggtctc cgacgggtac gacgatcagt tcacgacccg ccaccgacgg tcggtggtca
      661 cggggacggc ctcatccgtg gccaacgacg acgcggggac ctgtctcttc ttcggcagct
      721 acatccagag ctgaagtggg tgaccgatgg cgcggaagga tgcttggtcc caacacgcat
      781 cgttgatcct cctcggagtc ctgctgggcg ccatcatggt ggcgccggcg ttcgcgcacc
      841 acacgcctca gcacacgagg aagcagctcc gcacgctcga ccgggaggtc caggcgtacg
      901 acgtcagtca gggcctcgga gggccagacc aagcgactgg cgaaggtggg gaagctcgcg
      961 gtgaggggtc agtgcttgga tgacgcgggc acgaccaccg cgatcgtctt cgtctccgga
     1021 ccgaacggta ccgtcgtgac ggggtccagc acgagcatca tcatcggccc cggaacgacg
     1081 aaagacgatc ggagcatcga gaacgaggga gcgagcgcgt cctcgggcac ggaggtatcg
     1141 gacggctacg acgatcagta cacgctccgg taccggcgca tcgtcgtgac gggcaccgcg
     1201 tcgtcgacgg ccaacggcga cagcgggacc tgccggttct tcgggagcta catcaggagc
     1261 tgacaagggg ggccgcggga cgcgggacgc gggacgcggc ccccctcccc ccgccgcact
     1321 agactcgcct cgatgagcga gcccgtgcgg cggatccggg atcgggacca tgccctctgg
     1381 aagcccacgg agatcgtcga ccgtcttggg tggctgacga tcgccgacgc gattcggtcg
     1441 cgatcggcgg gctggagagc ttcgcggggc acgtggccgc cggtctcgac gatcgctctc
     1501 gagacaggct gaacgtggcg accctgctcg ggttcgggcc tcgcttcctc cactcgaggg
     1561 gacggttcca tgagggaggc tcgagctcgg tcgtgtgggg ccaggtggtg gacgagaggc
     1621 ggaaggtggg cggggagatc ccggggaagc cgtacacgtc cgggcgggtc aacctcgaca
     1681 ctctcatcaa ggaggcgagc tgatgcggat cgggatcgtg gggctgggcc ggatgggggg
     1741 gaacatgcgg gaccgcctgc ggggcgcggg ccacgaggtc ctggggtacg agcggaagga
     1801 gggacaccgc gacgtcgcct cctatgagga gctggtgggg gccctggacc cgccgagggt
     1861 cgcctggtcg atggtccctg ccggcgccgc caccgaggcg gtggtcggcg agctcgccgg
     1921 cctcctctcc gaaggggacc tcatcgtgga cggcgccaac tcgcacttcc gggactcgat
     1981 gcgccgggcc ggcgagctcg ccgaccgggg catcgcctac gtggacgccg gcgtctcggg
     2041 cggcgtctgg ggcctcgagg gcggatacgg cctcatggtc ggcggacccg acgacgccgt
     2101 ggcgagcatc tcgcccctcc tggacgcgct cgccacggag ggacactggg tccacgtggg
     2161 cccttcggga tcgggccact tcacgaagat gatccacaac gggatcgagt acggcatgat
     2221 gcaggcctac gccgagggct tcgagatcct ccaccggtcg gaggacttcc ccgacatcga
     2281 cctgcacgcg gtcgcggagg cctggcgcca cggcacggtg atccgctcgt ggctgctgga
     2341 tctgatcagc gccgcgctcg agcgcgatcc cgagctctcg gagctggccg cctgggtgga
     2401 cgactccggg ttcgggcgcg gcactgtctc gcaggcgatc gaggaggccg tccccgcgcc
     2461 ggcgatcgcg gccgccctct tcgcccgctt cgccagccgc gacgacagct cgttcgcgat
     2521 gcggctcgtg gccgcgctcc gccgcgagtt cggcggccac gccgtccagc cccccgattg
     2581 agcgagcgag ttgaccgcgc ggggggaccg cgcacagaat cgtggccccg gcacgaggag
     2641 gaccgatggc cgacgagccg acccagttcc tgcttcagtc cgacgacctt ccgagcgcct
     2701 ggtacaacat catggccgac gtcccgggag agccgctccc gccgctccac ccggggacga
     2761 aggagccggt gggcccggat gccttcagcg cgatcttccc catgtccctg atcgagcagg
     2821 aggtctcggc cgagcggtgg atcgacatcc cgggtccggt cctcgacgtc taccggctgt
     2881 ggaggcccac acccatgtac cgggcccggc gcctggagca ggccctggac acgccggccc
     2941 gcatctactt caagtacgag ggcacctcgc cggtcggctc ccacaagccg aacaccgcgg
     3001 tggcccaggc ctactacaac aaggaggacg gtacgaagct cctcaccacc gagaccgggg
     3061 ccgggcagtg gggctcggca ctcgccatgg ccgcgaggtt cttcggcctc gagtgcctcg
     3121 tcttcatggt gaaggcctcc tacgagcaga agccctatcg ccgcgtgctg atggagacgt
     3181 tcggagccga ggttcgtccc tcgccgacct ccaccaccga ggcggggaag aaggtgctgg
     3241 ccgagcaccc cgactccacc ggctcgctcg gcatcgcgat cagcgaggcg atcgaagcgg
     3301 ccgtccagga cgagacgacg aagtactcga tcggctcggc gctcaaccac gtgctgctgc
     3361 accagacggt gatcggcctc gagacccaga agcagatgga gatggcgggg gagaagcccg
     3421 acgtgatcat cggctgcgtg ggcggcggct cgaactacgc cgggttcagc tatcccttca
     3481 tggccgacaa gctggccggg acgctggacg ctcgcttcct ggccaccgag cccgccgcgt
     3541 gccccaccct cacccgaggg gagtaccggt acgacttcgc cgacacaggc gagatgaccc
     3601 cgctcgtccc catgtacacg ctgggccaca cgttcgtccc ggcgccggtg cacgcgggcg
     3661 gcctccgcta ccacggggat gccccctcgc tgtccgcgct ggtccgggcc ggcctgatgg
     3721 aggcgaaggc ctacacgcag aacccggtgt tcgaggccgc cacccagttc acggccgccc
     3781 aggggatcct cccagggccc gagccggcgc acgcgatccg ggcggtgatc gacgaggcga
     3841 tcgaggcccg cgaggcgggg gaggagaggg tgatcctctt caacctctcc gggcacgggt
     3901 tcttcgacct ccaggcctac gacgactacc tgaacggacg gctccccgag cacgagctgg
     3961 acgaggagga cctggcgcgt gggatggccg aggttccgga cgtgcccgtc cctgggtagg
     4021 agccgggcga ccgcatccga taccctgggg ggccgatggc caaggccgat ctgaacccgt
     4081 ccgacctgct cgacctggag cacctcctct ccgacgagga gaccgccgtg caggacaccg
     4141 ttcgcgggtg ggtgggcgat cgcgtcctcc ctgagatcgg cgactggttc gaggccggcg
     4201 agatcccctc gcgcgacctg gccaaggagc tgggcgcgat gggcctcctc gggatgcacc
     4261 tggagggcta cgggaccgcc ggcgcctccg cggtgatgta cgggctggcc tgcctcgagc
     4321 tggaggcggg cgacagcggg atccgctcgc tcgtctcggt ccagggctcg ctggcgatgt
     4381 tccccatctg gaagttcggg tccgaggagc agaagcagga gtggctgccc aagatggcgg
     4441 ccggggaggc gatcggctgc ttcgggctaa ccgagcccga cttcgggtcg aacccagcgg
     4501 gcatgcgggc ctccgcgacg aaggacggct ccgactggct cctcaacggg accaagatgt
     4561 ggatcacgaa cggcggggtg gccgacgtgg cgatcgtctg ggcccggacc gacgacgggg
     4621 tgcgcgggtt catcgtcccc accgacacgc ccgggttcac cacgaacgac atccacaaga
     4681 agatctcgct gcgcgcctcg gtgacgagcg agctgatcct ggagaacgtg cggctgcccg
     4741 acgacgcggt gctgcccgag gtcaccggca tgaagggccc gctctcgtgc ctcaacgagg
     4801 cccgctacgg gatcgtctgg ggctcgatgg gggcggcgcg ggcctgcttc gagagcgcgc
     4861 tggagtacgc gaagacccgc gtgcagttcg acaggccgat cggcggcttc caactgaccc
     4921 agcgcaagct cgtggacatg gcggtggagc tgcacaaggg gctgctgctg gcgctgcacc
     4981 tcgggcggat gaaggacgag aagcgggccc ggcccgagca cgtcagcttc gggaagctca
     5041 acaacgtgcg cgaggcgatc gagatctgcc gcgaggcccg cgggatcctc ggtgccagcg
     5101 gggtgacgct cgagtacccc gtgatccgcc acatgaacaa cctcgagtcg gtcctcacct
     5161 acgagggcac gaacgaggtc cacacgctga tcctggggaa ggccctcacc ggcctcgacg
     5221 ccttcgagta ggccatgccg ccgttccgtg ggtctgcggg gttcacggag cgctgggagc
     5281 acctcgacgt cggtctcgag gacggcgtct gcacggtcac gttctcgcgc ccggaccggc
     5341 tcaacgccct gaccttcgat gcgtacgccg atctgcggga cctgctggcg gagctgccgc
     5401 accgcgagga tgcccgcgtc gtgctgatca ccggcgaggg gcgcggattc tgctcgggcg
     5461 gtgacgtgca cgacatcatc ggagccctcc aggacatgga cccgcgcggg ctcctcgact
     5521 tcacccggat gacggggacc gtggtccagc ggatgcggga gtgcccgctc ccgatcgtgg
     5581 ccgcgatcaa cggcgtggcg gccggcgcgg gcgcggtgat cgcgctcgcc tcggacctgc
     5641 ggatcgccgc gcgttccgcc tcgttccggt tcctcttcac gcaggtgggg ctggcggggg
     5701 cggacatggg ctccgcctac cttctcccgc gcctgatcgg gaccgcccgg gccacggagc
     5761 tgctgctgct gggcgagggg gtggacgccg agcgcgcgct ggagattggg ctggtcacgc
     5821 aggtggtcga ggacgaggag ctggcgaagg cggcggggga cctggcgcgc cgcctcgcct
     5881 cgggcccggc gctggctcac gagacgacga agatgctgct gacgcgagag caggacatgg
     5941 gcctcgggcc gtcgatcgag ctcgaggctt tcgctcaggc cctcctcatg aagagcgagg
     6001 atcacgcgga gttctaccgg gcgttcctcg agggccggga cccgcggtgg agcggccggt
     6061 gaccgagctg ttcaacccct cggagctccc gaagccgagc ggcttctccc acgccgcgtc
     6121 cgcgtcgggg cggatggtgt tcgtggcggg ccagatcggt gaggggccgg acgaggggct
     6181 ccccgagggg atcgccgagc agatggacct cgcctgcgcc aacgtggtga cggcgctgcg
     6241 ggcggccggc gccgagcccg agcgggtcgt ctggatgcag atcttcacca ccgatatggc
     6301 ggcctaccgc gaggcgtcgc gggagatcgg ggaggcgtac cggaagcact tcggcaagca
     6361 cttccccgcg atggcgctcg tcgaggtgtc ggggctgttc gagccggccg cgctcgtgga
     6421 gctgaccgcg gtggcggtcg tgcccgacgg aggatgacct ggatcgctct ccgcgctatc
     6481 ggacgatgaa gtcccccttc atcttctccg cgatgtgcgc gtcgcagtag aagacgtact
     6541 tccccttggg aagcgcatcg acgtcgtaga cgatctccac cgggcctcgg atcagctccc
     6601 ctcggaacag cgggtggcgg aagaccggga cgccgctgat cgtctcgaac accgcgacgt
     6661 tgtgctccag gtcgtcctgg ttatcgagga cgatctggaa gggctggtcc gccggcgcga
     6721 ccaggcggtc cgtgtcgaag gcgatcccgc tcgcggtgat cctgacctgg gtcttgtcgg
     6781 ggtcgacctc ccgggagagg ctctcctcgc gcgcggcctc gctcgaggac acgggggtct
     6841 tgtcctcccc ctccccttcc tcgacggcgg ccggctctcc accgccgcac gccgtcaggg
     6901 ccatcagcgc ggccagcatc gctgccgatc tccatgccat ggctcgacct cctcgtgggg
     6961 gccccgtgtg ggccgttcgc cagcgacgct agcgacgccg agaggggcgg ttcatcgggg
     7021 agctccctca tccccgccga tgcgggcggc gagctgggtc cggttgtcca ccccaagctt
     7081 ggcgaggatg ttcgagacgt gccgctccac ggtcttccgg gagaggaaga gggaggcggc
     7141 gatctcgggg ttgctggccc ccgccgccac gaggcccgcc acctcacgct cgcgctcggt
     7201 gagctcgccc gcgcccgcgc cgcgcttcca cgtccggacg ccgagggccc gcagcctctg
     7261 ctcggcgagc cgctgctccg tgatcgcgcc gatgcgctcc gccgcccgcc cggcttcgcg
     7321 gagcacctcg gccgcccgac cccggtcgcc tcgccccacc atcgtcgcgg ccatctccag
     7381 gcgcgcccag aggatgtcca tcacgaggcc ctgacgctcc gcctcctcca ggaaggcctc
     7441 cagggcctcg gtccggccct ccgaagcctc gatcacgccc tcggccagca tggcccgcgc
     7501 ggcgagccac gcgtccgtgg tcagggctcg ccgacccgcc tcgagcgcga cccgcgcatc
     7561 ccccaggtgg ccgatccggg ccagcgcctc ggcgcccacg aggcgaacct cggagcggca
     7621 ccggccgcag ccggcggcct ccgcatccgc ctccgcaccg gcgagcagct cgcgtacctg
     7681 ggccgcgccc cctgacgggt cgaggcgggc catcgccgtc gccgcggcca tcctcgcgtg
     7741 gaggcgcatg tgggggtcgt gctcggctcc cgcctccgcc tgcagaccgg ccatccctcg
     7801 tcgccacgag ccccgcgaca gctccagcac gtggggcagc gtgatggcga agacccgggg
     7861 cggtcgggag acggcgatcc tcgcctggag ccctcggagc tcctccgaga gctcctgggc
     7921 gtcctccagg cgcccgaggg agtgcaggat gttcacgagc acccagccca cctccagcat
     7981 catccgcggg aagacgcgtc ggcgggcctc gtagtaggcg agccggagcg ccgtctccgc
     8041 ctcacgcgtg cggcccaggg cgcggagggc catccctcgc tgcccgatcg cccggaagcg
     8101 ctctacctca tcgacccctc tcgcggccgc ctccgtctcc tccgcgaggg cgagcatccg
     8161 ctcagggtcg tcggtggcga gcaggtcgtc cgacgccgcc agcagggcgc gaaggtacgc
     8221 gctgcgcgcg cgaggctcga gcgcctcggg gccctccatc gccgtccgcg ccccggcgag
     8281 cgcgcgtccg gccatcgccc gcgcctcctc cggacgcatc tcggcccagc ggagggcccc
     8341 ggcctccgcc gcgtcgatct cgatcgcgag cgaggcgtcg ccctccgcca gggcccgggc
     8401 ccggtccagg tggcgccgcg cctcgccggc ctccctcagc tccgtcgcgg cctcggcggc
     8461 ggccaggtgc atccgggcgc gcgcccgcgg atcgccgagc acgtcggcga tcgccgccgc
     8521 tcgctccagg gcaacctcgt gccggcccag ctccgccgcg agcgacacca gatgttccat
     8581 gagggctgcg gcctcctcgg gctcgtgctg atcggcgacc gcggccagcc tggcgagccc
     8641 atcgtcgccc aggagtcggc gacgcggcga ggtcgcgagc cgcagggaga ggtcgaggga
     8701 ggggagcccc gcctcccggc gatggtccag cgcctcgagg aggttggcct ccccggcgcc
     8761 ccggtgctcg aagacccggg ccaggcccgc gtggatcgtg gctcgactcg cgtcgggcac
     8821 ccgctcgacg gccgccgtcc ggatgagatc gtgggccagc gagacgccgt cctgcccgtg
     8881 caccgccagg cccgcccgga cgagatcgtc gatcgcctcc tccgctcgcc gtacctcgac
     8941 ccccagggcg tccgccgcct cgtcgggctc gagggggagc ccggcgaggg cgaggaggga
     9001 gagaaggcgt ccctgttggg ctcccaaccc ccgcagccgg ttctctagga ggacgcggac
     9061 gtcggtcccg cccgccgcta acagcgctcc gagccagaag ggagaccctc gagcacgctc
     9121 ccacgtccga accgcgtcct cgcggtccag gcccggggcg agggccgcgg cgagctcgat
     9181 cccctcctcc cggtcgagcc cggcgagctg gatgcggatc agccgaagga gccgctcgac
     9241 cgccgtggcg aaggtggtcg cttcgactga tgggcgcgtg gcgagcagga gcccgacggt
     9301 gtgtccttcg gccgccgctg cccgtaggag gtagtggcac agccctcggg tggcgtcgtc
     9361 gacccactgg agatcgtcca cggcgatgag cacgcgctcc cttcccgcga tcgcccggaa
     9421 cgccgcttcg cagaccctca aggtgtcagg cgcgtcggcg aacaccccca ggtcgaccgg
     9481 tccgtggtgc tccaccaggt cgaggcgcga gaggagggag cgggccgagg acagtgggat
     9541 gttccgctcc ggctcgtagc cgaggaccga gaggtgctcg aggtcggtac gggccagcgc
     9601 ctcctccagc aggcgggact tcccggtgcc gggatcgccc tcgatgaggc acgcggccgg
     9661 accctccgca tgctccaccg tccggagcac ggcctcgagg gcctcgagct cgcgggctcg
     9721 gccgacgaac ggcgccctca cggggagagc gtactcctgc gccgaacgtc ggggagccct
     9781 cagtcccggt agagctcgcg ggccacgacc tcgcgttgga tctcgctggt cccctcgtag
     9841 atgcggggcg agcgcacctc gcggtagaga tgctccaggg ggtggccccg cacgagcccg
     9901 cgagcgccgt ggacctggat cgcggcgtcc acgacccgct gggccgtctc ggtggcgaac
     9961 agcttcgcca tcgcgctggc cttcgtgacg cgctctcccc ggtcgtaggc gctcgccgct
    10021 gcgtacacga gcagtcgcgc cgcctccacc cgcgtggcca tctcgccgag ctgctgggac
    10081 acggcctggt gctcgcggag cggcctgccc atcgaggtcc gctcgtccgc gtaggctacg
    10141 gccaggtcga gggcgcgctg ggccatcccc acggcgaacg ccccgacgct cggacggaag
    10201 agatcgagcg ttcgcatggc cacgcggaag cccccgtcga gctccccgag gaggtccttg
    10261 ggcccgaccc ggacgccctc caggcggaga tgcccgatcg cgtgcggcgc cacgagctcg
    10321 acgtgctcgc cggagaggcc ctcggcgtcg ccggggacca cgaacgcgct gacgcccttg
    10381 gcccccgcgt ccgccgtggt ccgagcgaac agcgtgtaca cgtcggcgtc gggggcgttc
    10441 gagatgaagg ccttctcgcc ggtcagccgc cagccgtccc cgtcgggctc ggcgcggagc
    10501 tgcagcgcgg ccgcgtcgct cccgcccccc ggctcggtca gcgcgaaggc ggccacggcc
    10561 tcgccggccc gcacccgagg gatccagcgt tccacgatct ccgcctgccc cgactggagg
    10621 atcgggtagg ctcccaggcc ctggagcgcc atcgccgtct cggcctcggt cgactccatc
    10681 gccacggcct cgcgcaggag ggagagcgtc atcgcggaga cctcgctgtc ctcggggaac
    10741 atgaggggga gcagcccccg ctcggcgagc gcccggacca gcgggcggtt cacccgcccg
    10801 ggctccccct cgatctctgc caggcggtcg cgcgcgatcc cgcgcgcttc ctcgatcagc
    10861 cgccgctgct cgtcggtcag cgcgaacgcg tccatctcgc gcactctacc ggctggcacg
    10921 ggctccacgt aaccgggcct cgccccgggt acgatcgacc ggacatgggt ccgaggggag
    10981 ggaccgggga gcgtcggccc ctggttcccc tggatgccta cggcagggtc ctggcggcgg
    11041 tcctgctggc ggtggggtgg acgatcgcct ctcccgaggc agcgtggtcc cgcgccgtca
    11101 ccgttgcgct gttcgcggga accgtcttcg tcgcgttcgg cgcttcgggc gttcgggggc
    11161 cggtggcggg aggggtcctc gtggccctgg gcgtggcggt ggtcgcggcg gttctgtggg
    11221 aggcggccgg tgagccggcc gtgaccgtcg tagtcggcct cgccctcgtg ctggcctcgc
    11281 tcggagcgat cgtccgccgg ctggtggcgc atcccgttgt gaccacgagc accgtggtgg
    11341 gcgcgctgac
//
LOCUS       DASUTW010000242         2655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_855127, whole genome shotgun sequence.
ACCESSION   DASUTW010000242 DASUTW010000000
VERSION     DASUTW010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855127"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..504)
                     /locus_tag="VF058_08325"
     CDS             complement(55..504)
                     /locus_tag="VF058_08325"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6208347.1"
                     /translation="MSGIDVTASTIVRAPREVVAAYVTDHRNDPVWIGGITESQLEGD
                     PPVAVGSRVRRVASFLGKRIEYVNEVVRLDPGSVLEMRSVKSPFPMVVTYSFEDADGG
                     TRTSVRVQGDPSALYRLAGPLLARQVKRSVQGDVERLREILEKGRSS"
     gene            619..1611
                     /gene="pip"
                     /locus_tag="VF058_08330"
     CDS             619..1611
                     /gene="pip"
                     /locus_tag="VF058_08330"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018742196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEX6208348.1"
                     /translation="MDSGEADRARATRRGLYPNLEPHARGVLDVGDGHHVSWESSGNR
                     DGHPAVVLHGGPGSGCRPWHRRLFDPAGYRIVTFDQRGCGLSTPHAADPGVDLSTNTT
                     GHLLADLERVRTHLGVDRWLVTGGSWGSALALAYAERHADRVRAMVLWGVTAARRSEF
                     DWLFRGGAGAFFPEQWERLTNAIPNDKRHLDVVDAYAELLFDPDPEVVRRSAEGWCRW
                     ESATPSWPPTTGLDERFEDPRFALGLARLVTHYVRHDAWLPNDDVFRRLEVLAGIPAL
                     LIQGRFDFQSPLGSAWAIHRRWPSSELIVVDEAGHAATTGITDQIVEATDRLGR"
     gene            complement(1627..2070)
                     /locus_tag="VF058_08335"
     CDS             complement(1627..2070)
                     /locus_tag="VF058_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6208349.1"
                     /translation="MTGRSEGVWFEVEERIEATPAEVFEHLIDPARYVRWMGAEAVLE
                     PRPGGTYRVHIPHRGVALGEFVTVEAPHRIVFTWGWDGDDVVPLGSSTVEVTLHERDG
                     ATLVRLRHSGLPDEESGRAHEGGWRKYLGRLDAVTTGGDPGPDLP"
     gene            complement(2067..2372)
                     /locus_tag="VF058_08340"
     CDS             complement(2067..2372)
                     /locus_tag="VF058_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6208350.1"
                     /translation="MDQLTFDAISSPRRRDILRLVWDRELAAGEIAAHFDVSWPAISQ
                     NLNVLRRAGLVTERRDGRRRLYRADRKALGPLAAALREMWRADLRALKREVERGRRR"
     gene            2457..>2655
                     /locus_tag="VF058_08345"
     CDS             2457..>2655
                     /locus_tag="VF058_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208351.1"
                     /translation="MGREMMFAVDIEAEPAAVFEALSTEPGLESFWTSNCDARPEVGS
                     VARFGFGKAPMDLRMRIDVLEP"
BASE COUNT          373 a          934 c          934 g          414 t
ORIGIN      
        1 ggcaggtctc gagctcgccc tcgcgaatcg cgaagtgagc cgttccgtgg atcgtcacga
       61 cgaacgcccc ttctcgagga tctccctgag ccgctcgacg tcgccctgaa ccgaccgctt
      121 gacctgccgg gccagcagcg gtccggccag gcggtacagg gcgctcgggt cgccctggac
      181 gcggacgctg gtccgggtcc cgccgtcggc gtcctcgaac gagtaggtga ccaccatcgg
      241 gaaggggctc ttcaccgacc gcatctcgag caccgatcca gggtccagac gcacgacctc
      301 gttcacgtac tcgattcgtt tgcccaggaa cgaggccacc cgccggaccc gggacccaac
      361 cgcgacgggc ggatcgcctt cgagctgaga ctcggtgatc ccgccgatcc agacggggtc
      421 gttgcgatgg tccgtcacgt aggcggccac cacctccctc ggcgcccgca cgatcgtcga
      481 ggcggtgacg tcgatcccgc tcatcgatgc ctccatcggg tctcaggcaa cattcaatag
      541 aacggttgaa tatagggcga tcggggtggg tgtgtcgagg cggcccgaca ccctggggag
      601 gtgtgccaga gtgccgccgt ggactcgggg gaagcggatc gggctcgggc gacgcggcgc
      661 ggcctctacc cgaacctcga gccgcacgcc cgcggcgtgc tcgacgtcgg cgacggccac
      721 cacgtgtcct gggagtcgag cgggaaccgg gacgggcatc cggccgtggt gctgcacggc
      781 gggccgggtt cgggctgccg gccatggcat cgacgcctgt tcgatccggc tgggtatcgc
      841 atcgtgacgt tcgaccagcg tggctgcgga ctgagcaccc cgcacgctgc ggaccccggg
      901 gtcgatctct ccaccaacac caccgggcat ctgctcgccg acctcgagcg agtccgcacg
      961 catctgggcg tcgatcgctg gctggtgacc ggcggctcgt gggggagcgc cctcgctctg
     1021 gcctacgccg agcgacacgc ggaccgggtg cgggcgatgg tgctgtgggg cgtgaccgcg
     1081 gcacgtcggt ccgagttcga ttggctgttt cgtggcggcg ccggcgcctt cttccccgaa
     1141 cagtgggagc gcctgacgaa cgccatcccg aacgacaagc ggcatctcga cgtggtcgac
     1201 gcctacgccg agctgctgtt cgatcccgac cccgaggtcg tgcggcggtc cgcggagggg
     1261 tggtgccgat gggaatcggc cacgccctcc tggcccccca cgacgggtct cgacgagcgt
     1321 ttcgaggacc cacggttcgc gctcggcctt gcccgactcg tgacccacta cgtccgtcac
     1381 gacgcctggc tgcccaacga cgacgtgttt cgccggttgg aggtcctggc ggggatcccg
     1441 gccctcctga tccaggggcg gttcgacttc caatccccgc tggggtctgc ctgggcgatc
     1501 caccgccgct ggccgagctc cgagctgatc gtcgtggacg aggcgggcca tgcggccacc
     1561 acggggatca ccgatcagat cgtcgaggcc accgaccgcc tgggccgctg acggctgggc
     1621 cgttggctac gggaggtccg ggccggggtc acccccggtg gtgacggcgt ccagtcggcc
     1681 gaggtacttg cgccagccgc cttcgtgggc ccgccccgac tcctcgtcgg gcaagcccga
     1741 gtggcgcagg cgaacgaggg tggcgccgtc gcgctcgtgc agcgtgacct cgacggtgct
     1801 ggagcccagc gggacgacgt cgtctccgtc ccatccccag gtgaacacga tccggtgggg
     1861 agcttcgacc gtcacgaact cgccgagcgc aacaccccgg tgggggatgt gcacgcggta
     1921 cgtgccaccg ggcctgggtt cgagcacggc ctccgcgccc atccaccgga cgtaccgggc
     1981 ggggtcgatc aggtgctcga agacctcggc gggggtggcc tcgatccgct cctcgacctc
     2041 gaaccacacg ccttcggacc gacccgtcat cgccggcgac cccgctcgac ctcgcgcttc
     2101 agggcgcgca ggtccgcccg ccacatctcg cgcagcgccg cggccagcgg gcccagcgcc
     2161 ttccggtccg cccggtacag gcgccgccgt ccgtcccggc gctcggtgac cagcccggcg
     2221 cggcgcagca cgttcaggtt ctgcgagatc gccggccagc tcacgtcgaa gtgcgccgcg
     2281 atctcgccgg cggccagctc ccgatcccac acgagccgga ggatgtcccg ccggcgcggg
     2341 ctcgagatcg cgtcgaacgt gagctggtcc atcagcgtta tttaagcatc accttcagct
     2401 tgacggacga tgaagctcac tcttaaatgt ggtgctccga gatggggagg tgggtggtgg
     2461 gacgggagat gatgttcgcg gtcgacatcg aggccgagcc ggcggccgtg ttcgaggcgt
     2521 tgtccacgga accgggcctc gagtcgttct ggacgtccaa ctgcgatgcc cggcccgagg
     2581 tcgggtccgt ggcccggttc gggttcggca aggcgccgat ggaccttcgg atgcggatcg
     2641 acgtgctcga gcccg
//
LOCUS       DASUTW010000243         6975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_466793, whole genome shotgun sequence.
ACCESSION   DASUTW010000243 DASUTW010000000
VERSION     DASUTW010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6975
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466793"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..140
                     /locus_tag="VF058_08350"
     tRNA            67..140
                     /locus_tag="VF058_08350"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:101..103,aa:Arg,seq:acg)
     gene            474..1796
                     /locus_tag="VF058_08355"
     CDS             474..1796
                     /locus_tag="VF058_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6208352.1"
                     /translation="MTTKTLLRGGCVLTLGAKTPNYMQADVLIDEGRVAEVGPGLRAR
                     DAEIVDATDTIVMPGFVDTHRHAWTSLLRNLGEGGSNGHADVPAAASTIPYEPDDVYA
                     ATLIGLLGAVESGITTVVDWSDVRADDGPASEAALQAHADAGLRTVFVQVPGDGDDEG
                     ALRESLARLAPRAEPSTSIALGSAAPFDADRLARRWAVARELGLRIYGHLASGSAGAG
                     TVADLASRGLLGEDVTLVHCSVLDEKDLDAVADSGASVSLSPSSDMAEGSGSPPIQEL
                     IDHGIRPGLGVDHERLAPGDAFAQMRATISLQHATVFDLKLAGKAGLPKLMSTRDVIR
                     YATSEGARVAGLGGVTGSIEPGKQADIIVLRTDRPNIFPVNDPIGAVVWGMDTSNLDW
                     VFVGGRALMREGVLEADVARARDLASRARERVGVASGIVPGAAGGGEG"
     gene            1793..2842
                     /locus_tag="VF058_08360"
     CDS             1793..2842
                     /locus_tag="VF058_08360"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6208353.1"
                     /translation="MKTERRPPSSAITALVVGSRYMPVYLAIGLLVIVAWIWAPETLS
                     GPGLRAIAPFGTFLAIAALGQMLVVMTGGIDLSVPGSFTLAAMITVGVGQASDERLWP
                     AIGTALLMVALVGLVNGILIGGLGLNALIVTLAVGQIVSGIAYRYYTGVAIQTPVPPA
                     LSEWTSARFLGVTRTFWVGVLLTLALIVIFRFTTPGRRFQVVGANPMASWIAGLRVNV
                     NQVSAYVVAAVLYGVAGILLAAFLGTPSISIGVPYLLGPIAAVVIGGASLTGGLASPL
                     STWAAAFFLAALNQMMRVMGLPTALQFVVFGLVIIGGMLVSGDRIIRGVEHLLRERRR
                     SDHAQAIGGPTDGTG"
     gene            2916..4202
                     /locus_tag="VF058_08365"
     CDS             2916..4202
                     /locus_tag="VF058_08365"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX6208354.1"
                     /translation="MQRFKFVWIVAVLVLVAAACGGDGEGTTTTGGDDGDTTTTGEGP
                     LAEYGESTDADPALVEKALGPVEDVPDIVLASIARADQDLDDATIATAMDCWNNVECE
                     TGSGGELTMGVADGGRGNVWRQVTHMEAILQALTYPEIGTIVSRDAQWAEDPAVAEAD
                     IRFLVQRGVDFIIGYPDQGTNIAGAILEAEAAGIPYIPYSAGWVGLPDQEGALVPGQD
                     YLTIVGEDLCALGESFAEVLNQEIGSGTIGVMGGTPGNALSAGWQQCLTPALNDGIEL
                     VGPPADTYWVNDFALQTVLGWLSTDPEIKGYAYEYADGMYTALDAYEQLDVPVEDITI
                     AVRTDEQTLFCEWAERSEPSFKIYFSAGGNFQSRVAVTAAMMHLKGAEIPPEIVVPHV
                     MREVTPEDCDPDRVTPTVSGTSLVPDEVLAEMYANG"
     gene            4199..5782
                     /locus_tag="VF058_08370"
     CDS             4199..5782
                     /locus_tag="VF058_08370"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX6208355.1"
                     /translation="MSGTGGGSAARDVVLELRGVYRQFGAVRALTDVSFDCRPSEIHA
                     VVGENGSGKSTLLGIASGFVDPDRGTILIGGKRLRRDSPAEARKLGLAMAYQDTSLIL
                     EQPVKNNLYLAAPPDKRPPFHRRKRWARRLLAELDLDLELFPDMPAGALSLSERQQFE
                     VAKALVTDPLVLLLDEPTTALGPHEVEALHRTLEACRDRGVGVVYVSHRLPEVLEVAD
                     RITVLRDGRSQGTFEAREVAEPDLVDLIVGRPFEAAFPQPTPAAAEAREVLVVDGLQG
                     QSFGPVTLTLESGEIVGVAGAEGNGQPQLFDCLAGRQPPKAGRVVVDARELMLISTHE
                     AVRAGIMMLPGDRKHEALMPVLGVRVNSTIQSLRRFGVLGFLRRRKERTSVRDLIERL
                     EIRTPSLEQPVQFLSGGNQQKVAVSRSFLREPSVILAYEPTQGVDVGSRFDIYEALRS
                     RADAGAGILVKSSDPLELSGLCDRVLVMSRGQIVEEIPGDELDERRIVEAIVRGPGMS
                     RAARSPLGVAMPKAGRDTA"
     gene            5779..>6975
                     /locus_tag="VF058_08375"
     CDS             5779..>6975
                     /locus_tag="VF058_08375"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6208356.1"
                     /translation="MSARIDAARRLVRKVLRDARDVNKWRKIVRFRLWMPVALQVLLL
                     IAIASYTTARFPGFINATNVTNILILAAPLAVAAMGQTHALLAGYLDLSVGAMVTFGV
                     VIASFLIGPDATASQIALGGGVILLAGLGLGVVNTVLVLGFKIPSIIATLATLSILNG
                     ISLTLRETPGGIINRDFTASLGTTLGPLPISFLLVVLAAGALDYWLHASGSGLQLRST
                     GFDERSAKRSGVRTKWVQGRALVLSALFAAVASFLVMTRSGVGNAAIGDSYVLNSITA
                     AVLGGAALSGGRATFVGTLVASVLLALIISALPFLGLSGEHGLMIIGVLVLLAIVLFQ
                     AGDMKELVKRNYKRARRLAIGSRPPKEAEIPDFYPSSTDFGAAPTGRILIRGGTVLSM
                     DPSVGDF"
BASE COUNT          985 a         2386 c         2554 g         1050 t
ORIGIN      
        1 cgcgggattg ctaatcccgt tgggggttca agccccctcg cgcgttcgaa tcgcgccgcc
       61 tccgctgcgc ccgtagctca attggataga gcgtctgact acggatcaga aggctggggg
      121 ttcgagtcct cccgggcgcg ctccatcatc ctcgatcctc gtggaggtcc acgaggatct
      181 ccacgagctg gatcacggtc gctaccccca ccacagcgag actactaccg ttgtgtagca
      241 tcgctgatcg ctcccgagcg agccgcgagc cgccggcaag gcccggacac ctctcgccat
      301 cgccaggtcg cgcttctggc gcacccgaga caagatgtgt agtgttactg aacggtgagg
      361 gggactcgct cgagagtgag catcggtccg gagaggggga cgatggcggg caccgtcatc
      421 agcggcttcg acgcggcccg ggcgtcgatc cgcgccacgc cccggagcgg cgcgtgacca
      481 cgaagaccct cctgcgcggc ggatgcgtcc tcaccctggg ggcgaagacc cccaactaca
      541 tgcaggccga cgtcctcatc gatgagggac gggtggccga ggtggggccc ggcctccgcg
      601 cacgcgacgc ggagatcgtc gacgccaccg acaccatcgt catgcccggg ttcgtcgaca
      661 cccaccgtca cgcgtggacg tcgctgctac ggaacctcgg tgagggcggg tcgaacggac
      721 acgcggacgt ccccgcggcg gcctccacca tcccgtacga gcccgacgac gtctacgcgg
      781 ccaccctgat cggcctgctc ggcgccgtgg agtcggggat caccaccgtc gtggactggt
      841 cggacgtccg ggcggacgac ggcccggcct cggaggcggc gctccaggcg cacgccgacg
      901 ccgggctgcg cacggtgttc gtccaggtcc ccggcgacgg ggacgatgag ggtgccctcc
      961 gcgagtcgct cgcccgtctc gccccccgcg cggaaccgtc gacctccatc gcgctcggtt
     1021 cggcggcgcc gttcgacgcc gaccgtctcg ctcgccgatg ggcggtggcc cgggagctcg
     1081 gcctgcgcat ctacggtcac ctggcgtccg gttccgcagg ggccgggacg gtggccgatc
     1141 tggcgagccg gggacttctg ggcgaggacg tcacgctggt ccactgctcg gtcctcgacg
     1201 agaaggacct cgacgcggtc gccgactccg gcgcgtcggt ctcgctgtcc ccctcgagcg
     1261 acatggccga gggctccggg tccccgccga tccaggagct gatcgatcac ggcatcaggc
     1321 cggggctggg ggtcgatcac gagcgcctgg ctccaggcga cgccttcgcg cagatgcggg
     1381 ccacgatctc gctccagcac gcgaccgtct tcgacctcaa gctggcgggg aaggccggtc
     1441 tccccaagct gatgagcacc cgggacgtga tccgctatgc cacctccgag ggtgcccgcg
     1501 tcgcgggact gggcggggtg accggctcga tcgagcccgg caagcaggcg gacatcatcg
     1561 tgctgcgaac cgaccggccc aacatcttcc cggtgaacga tccgatcggc gcggtggtat
     1621 ggggcatgga cacctccaac ctcgactggg tgttcgtcgg cggccgcgcg ctgatgcggg
     1681 agggcgtcct ggaagccgac gtggcgaggg cccgcgatct ggcctcccgg gcgcgagagc
     1741 gggtgggggt cgcgtccggc atcgtgccgg gcgcagcggg cgggggcgag gggtgaagac
     1801 cgagcgccgc cccccctcgt cggcgatcac cgccctcgtc gtgggctccc ggtacatgcc
     1861 cgtgtacctg gcgatcgggc tcctggtgat cgtggcgtgg atctgggcgc ccgagacgct
     1921 gagcggcccg gggctgaggg ccatcgcgcc gttcggcacc ttcctggcca tcgccgcgct
     1981 gggccagatg ctggtggtca tgaccggggg catcgacctc agcgtccccg ggtcgttcac
     2041 gctcgcggcg atgatcacgg tgggggtggg gcaggcctcc gacgagcgcc tgtggccggc
     2101 gatcggcacc gccctgctga tggtggcgct cgtagggctg gtcaacggca tcctgatcgg
     2161 tgggctgggc ctgaacgcgc tcatcgtgac gctggcggtg ggccagatcg tcagcggcat
     2221 cgcgtaccgc tactacacgg gggtcgcgat ccagaccccg gtgccgccgg cgctctccga
     2281 gtggacatcg gcgaggttcc tgggggtgac gaggaccttc tgggtcggcg tcctcctgac
     2341 gctcgcgctg atcgtgatct tccggttcac cacccccggc cgcaggttcc aggtggtggg
     2401 ggcgaacccc atggcgtcgt ggatcgcggg tctgcgcgtg aacgtcaacc aggtgtccgc
     2461 ctacgtggtg gccgcggtcc tctacggggt tgctgggatc ctgctcgccg ccttcctggg
     2521 gacgcccagc atctcgatcg gggttccgta cctcctgggc ccgatcgcgg cggtggtgat
     2581 cggcggcgcg tcgctaacgg ggggcctggc cagtcccctg tccacgtggg cggcggcctt
     2641 cttcctggcg gcgctcaacc agatgatgag ggtgatgggc ctgcccacgg ccctgcagtt
     2701 cgtggtattc gggctggtga tcatcggcgg catgctcgtc tccggcgatc ggatcatccg
     2761 cggcgtggag cacctgctcc gcgagcgccg gcggtccgac catgcacagg cgatcggcgg
     2821 tcccacggat gggacggggt gagaaaggag gagcggatct ggcgagccta ccgatgcgcg
     2881 agagctggaa gcgtccactg agagggggga gcagcatgca acggttcaag ttcgtgtgga
     2941 tcgtggcggt cctcgtgctg gtcgccgcgg cgtgcggcgg cgacggggag gggaccacga
     3001 cgaccggcgg cgacgacggc gacacgacga ccaccggcga gggcccgctg gccgagtacg
     3061 gggagagcac cgacgccgac cccgcgctcg tggagaaggc cctgggcccg gtcgaggacg
     3121 tgccggacat cgtgctggcc tccatcgccc gcgcggacca ggatctcgac gacgccacga
     3181 tcgccacggc gatggattgc tggaacaacg tcgagtgcga aaccggcagc ggcggcgagc
     3241 tgaccatggg ggtcgccgac ggaggccggg gcaacgtctg gcgccaggtg acgcacatgg
     3301 aggcgatcct ccaagcgctc acctacccgg agatcggcac gatcgtctcc cgcgacgccc
     3361 agtgggccga ggacccggcc gtggcggagg ccgacatccg gttcctggtc cagcggggcg
     3421 tcgacttcat catcggctac ccggaccagg ggacgaacat cgcgggcgcg atcctcgagg
     3481 ccgaggccgc cggcatcccg tacatcccct actcggccgg atgggtcggg ctccccgatc
     3541 aggagggcgc gctcgtaccg ggccaggact acctcacgat cgtgggcgag gacctgtgcg
     3601 cgctcgggga gagcttcgcg gaggtgctca accaggagat cggctccggc acgatcggcg
     3661 tgatgggtgg gacgcccggc aacgccctgt ccgccggctg gcagcagtgc ctcacgccgg
     3721 ccctcaacga cgggatcgag ctggtcggtc ccccggcgga cacgtactgg gtgaacgact
     3781 tcgccctcca gacggtcctc ggatggctgt ccacggaccc agagatcaag gggtatgcgt
     3841 acgagtacgc cgacggcatg tacacggcgc tcgacgcgta cgagcagctc gacgtccccg
     3901 tcgaggacat cacgatcgcg gtgcggaccg acgagcagac gctgttctgc gagtgggccg
     3961 agaggagcga gccgagcttc aagatctact tctcggccgg cggcaacttc cagtcgcggg
     4021 tcgcggtcac cgcggcgatg atgcatctga agggcgccga gatcccgccc gagatcgtcg
     4081 tcccccacgt gatgcgtgag gtgaccccgg aggactgcga tcccgaccgg gtgaccccga
     4141 cggtgtcggg gacgtcgctc gtgcccgacg aggtgctggc ggagatgtac gcgaacggat
     4201 gagcggtacc ggaggagggt cggcggccag ggacgtggtc ctcgagctgc ggggcgtcta
     4261 ccggcagttc ggcgcggtgc gagcgctgac ggatgtcagc ttcgactgcc gccccagcga
     4321 gatccacgcc gtggtgggcg agaacggctc cggcaagtcg accctcctcg ggatcgccag
     4381 cgggttcgtg gaccccgatc ggggcacgat cctcatcggc gggaagcgcc tccgccggga
     4441 ctccccggcg gaggcgcgca agctcggcct ggccatggcg taccaggaca cctcgctgat
     4501 cctggagcag ccggtcaaaa acaacctgta cctggcggcg ccgcccgaca agcgacctcc
     4561 gttccaccgg aggaagcgtt gggcgcgccg tctcctggcc gagctggacc tggacctcga
     4621 gctcttcccc gacatgccgg cgggggccct ctcgctgtcg gagcgacagc agttcgaggt
     4681 ggccaaggct ctggtgaccg acccgctcgt cctgctgctg gacgagccga cgacggcgct
     4741 ggggccccac gaagtggagg cgctgcaccg gacgctcgag gcgtgccgcg accgcggcgt
     4801 cggcgtcgtg tacgtcagcc accgcctgcc ggaggtcctg gaggtcgccg accggatcac
     4861 ggtgctgcgg gacgggcgga gccagggcac cttcgaggcg agggaggtgg ccgagcccga
     4921 cctcgtggac ctcatcgtcg gccggccctt cgaggccgcc ttcccgcagc cgacaccggc
     4981 ggcggcggag gcccgtgagg tgctcgtcgt cgacggcctc cagggccagt cgttcggtcc
     5041 ggtgaccctc acgctggaga gcggcgagat cgtgggcgtg gccggggcgg agggcaacgg
     5101 ccagccgcag ctgttcgact gcctggccgg gcgacagccc ccgaaggcgg gcagggtcgt
     5161 ggtcgacgca cgggagctga tgctgatctc gacccacgag gccgtccggg cgggcatcat
     5221 gatgctgccc ggcgaccgga agcacgaggc gctgatgccg gtgctcgggg tgcgggtgaa
     5281 ctcgacgatc cagtcgctgc gacgcttcgg ggtgctcggg ttcctccgga ggaggaagga
     5341 gcggacgagc gtgcgggacc tgatcgagcg gctggagatc cggaccccct cgctcgagca
     5401 gcccgtccag ttcctctccg gcggcaacca gcagaaagtg gccgtctcgc gctccttcct
     5461 gcgcgaaccc tcggtgatcc tggcctacga gccgacccag ggcgtcgacg tgggctcgcg
     5521 gttcgacatc tacgaagccc tccggtcgcg ggccgacgcg ggggcgggga tcctcgtgaa
     5581 gtccagcgat ccgctggagc tctcgggcct ctgcgaccgg gtcctggtga tgtcccgagg
     5641 gcagatcgtg gaggagatcc cgggggacga gctcgacgag cgccggatcg tggaggcgat
     5701 cgtccgaggc cccgggatgt cgcgggcggc gcggtccccg ctcggcgtgg ccatgccgaa
     5761 ggcggggcgg gacacggcgt gagcgctcgc atcgacgccg ccaggcggct ggtccgcaag
     5821 gtcctgcgag acgcccgcga cgtgaacaag tggcggaaga tcgtccgctt ccgcctctgg
     5881 atgccggtcg cgctgcaggt gctgctgctg atcgcgatcg cttcgtacac gaccgcccgg
     5941 ttcccgggct tcatcaacgc gacgaacgtc acgaacatcc tcatcctcgc cgcgcccctg
     6001 gccgtggcgg cgatgggcca gacccacgcg ctcctcgccg gctacctcga cctgtcggtg
     6061 ggtgcgatgg tcacgttcgg cgtggtgatc gcctcgttcc tgatcggccc cgacgccacg
     6121 gcctcccaga tcgcgctcgg ggggggcgtg atcctcctgg cggggctcgg gctcggggtc
     6181 gtcaacacgg tgctcgtgct cgggttcaag atcccgtcca tcatcgcgac gctggccacc
     6241 ctcagcatcc tcaacgggat ctcgctgacc ctccgcgaga cgccgggcgg catcatcaac
     6301 cgggacttca ccgcgtcgct gggcacgacg ctcggtccgc tcccgatcag cttcctcctc
     6361 gtcgtgctgg cggccggggc gctcgactac tggctgcacg ccagcggctc gggcctccag
     6421 ctgcgctcca cggggttcga cgagcggtcg gccaagcgca gcggcgtgcg cacgaagtgg
     6481 gtgcagggac gggcgctcgt gctctcggcg ctcttcgccg cggtcgcctc gttcctcgtc
     6541 atgactcgct cgggcgtggg gaacgcggcg atcggggaca gctacgtcct gaacagcatc
     6601 accgcggccg tcctcggcgg cgccgcgctc tcgggcgggc gggccacgtt cgtggggacg
     6661 ctggtggcgt cggtcctgct ggccctgatc atcagcgcgc tgccgttcct cggcctcagc
     6721 ggggagcacg ggctgatgat catcggcgtg ctcgtcctgc tcgccatcgt gctgttccag
     6781 gccggcgaca tgaaggagct cgtgaagcgg aactacaagc gggcccgccg tctcgcgatc
     6841 ggcagccggc cgccgaagga ggccgagatc cccgacttct acccctcaag caccgacttc
     6901 ggcgcggcgc cgacggggag gatcctgatc cgcggcggga ccgtgctgag catggacccc
     6961 tcagtgggcg acttc
//
LOCUS       DASUTW010000244         7089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_467026, whole genome shotgun sequence.
ACCESSION   DASUTW010000244 DASUTW010000000
VERSION     DASUTW010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7089
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467026"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VF058_08380"
     CDS             complement(<1..225)
                     /locus_tag="VF058_08380"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX6208357.1"
                     /translation="MSVEILPVAGLPEIGEDDDLAALIADAAELKDGDVVAVTQKVVS
                     KAEGRLVPEGEDWVGREAQRVVARRGDLVIA"
     gene            complement(222..1151)
                     /gene="cofD"
                     /locus_tag="VF058_08385"
     CDS             complement(222..1151)
                     /gene="cofD"
                     /locus_tag="VF058_08385"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEX6208358.1"
                     /translation="MRVTALAGGVGAGKLLRGLVRAVDPPDVTVIGNVGDDMVHLGLH
                     VSPDLDSVAYWLSGRMDRERGWGRAEESFRAMQELGRLGGQAWFSLGDRDLATHLHRT
                     QRLNEGATLSEVTAELCEALGVPCRVLPVTEDPVRTWIDVEGGSLPFQMYWVAHRAEP
                     KVERVRFEGAEKARPAPGTLAAIAEADAIVICPSNPVVSVMPILAVPGVHEALRERKD
                     RVVGISPIVGGTVLQGMADKLMPAMGLEVSAAGAAEAYRGLLAAWVVDERDRDLAPKI
                     EERESLRVGVTDTIMVDDEASERLARFALELLG"
     gene            1202..3664
                     /locus_tag="VF058_08390"
     CDS             1202..3664
                     /locus_tag="VF058_08390"
                     /EC_number="2.5.1.147"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009479191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional FO biosynthesis protein CofGH"
                     /protein_id="HEX6208359.1"
                     /translation="MAPIPAEVRRALARAEAGKSLSLGDVTALLAARGEELDRLLAVA
                     SRLRELGWGRTITYSPKVFIPLTMLCRDHCHYCTFAKPPARIEAPYLTPEQAVEIAEA
                     GRRAGCTEALFTLGDRPEDRYPEARAWLDERGHASTLDYVREVAIRVIEETGLLPHLN
                     PGVMSWEEMARLKHVSVSMGLMLEQASDRLLEKGLPHHGSPDKVPAVRLRMIEEAGRL
                     SIPFTTGMLIGIGETIEERAETLLAIREAERRYRHVQEVIVQNFRAKPRTRMRDHPEP
                     DRDDLLAAIATARVVLGPRVSVQAPPNLSDEGFPRLLDGGLNDWGGVSPVTPDHVNPE
                     RPWPKLERLRAATEARGFALRPRLPVYPRYVLKPDPWISGKMRAPLQALAGPDGYPSG
                     RRPEPIPWQDPDVQWKPREIDLAFAKAAGAGLREDALDVYGDFEQLDVTRRWAQERVT
                     PERLDAEIRGALGRAAGGLPITDDEALALFRAEGPALDALAEVADDLRRETVGEEVTY
                     VVNRNINFTNVCYTGCRFCAFAQREIDPESYTLTLHEVADRAEEAAAAGATEVCMQGG
                     LHPDLPGTFYFDLLDAVKARVPEIHVHAFSPMEVLNGATRLGISFREFLQECRRRGLG
                     SIPGTAAEILDDDVRWLLTKGKLPADTWEEIVRTAHEVGLPSSSTIMYGHVDAPPHWV
                     AHIRRLAGLQDDTGGFTEIVPLPFVHQNSPIYLAGKARPGPTHEENRRMHAIARILLH
                     GLIPNVQVSWVKLGLEASQLMLRGGANDLGGTLMEETISRMAGAEWGIRKEPEELEQA
                     IRTIGRVPAQRTTTYGRAAARL"
     gene            3751..4005
                     /locus_tag="VF058_08395"
     CDS             3751..4005
                     /locus_tag="VF058_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015772132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX6208360.1"
                     /translation="MHVILGRGAVHHYIPWQERARCREFDPEIFFPEKGGSSREAKRI
                     CAQCEVRIECLKYALRHDERYGVWGGMSERERRRLKRMAS"
     gene            complement(3986..4336)
                     /locus_tag="VF058_08400"
     CDS             complement(3986..4336)
                     /locus_tag="VF058_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208361.1"
                     /translation="MLFRPDDFRRAAVFLRAAVFLLAVAFLRPVAFLRAAVFLLAVAF
                     LRPAVFRRAVVFLLAVAFLRPAVFLRAAAVFLRAGAFLALDLFLPVAFFLAEDLFRAG
                     GIGHPPLPIRTPSA"
     gene            4484..>7089
                     /locus_tag="VF058_08405"
     CDS             4484..>7089
                     /locus_tag="VF058_08405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6208362.1"
                     /translation="MLAVLVVKDGADWLKRSLAALARQSYDRLGVLAVDNASTDGSGE
                     LLRDLLGPRRVVSLRENVGFPGAVARAMEIPAAREADFLLLLHDDTVLHPDAVARLVA
                     AAEGGVGVVGPKVLDGERRVLREIGYTIDRFGYRYSALEEGEIDQGQHDSPREVLFVS
                     GTAMLVARDAWLRAGPPDARLRPSHGDLDFCWRVRIAGFRVVMDPSAVVYHEAAGDAG
                     LREGARSDRTRYHEERSALTAMLKNYRLGTLLWVMPMYLVQGVAKVVLFLLTRRFADA
                     RQVLGAWAWTLWRFPGTVRRRVRLHAVRAVKEREVTATMAPTSARLRRWAGQVSAVLF
                     PARGASELAPEEEIALVPLRQRVGGFLGRNPAPVALGIGLLLTVVAFRGVLFASPLEG
                     GAIPTFPDSAREVFAAYLDGTVPAGFGGADGASPALVPLGLLSVLTLGNPRTLAWIIV
                     ALTPLLAGFAAYRAALRRTGAPGAAVAAGASYALSAVVLWAASEGRVGEMAFLIGLPW
                     ILGRIADPFERPVGPPLRWALGTAVVLAITGSFFPAAWAPVAIATVVTLIVGGRRERR
                     LSGAGIALATLVMAAVLAFPFVLTLARVGVGRSAELGIAAEFGSILRLSPGEAPGSWL
                     PALFLPVGGVLGFALATQRAAAWRGLVSASLALPLAWLAAAGYLPSAAAEPIAYLGLA
                     AFTLSALVGVGFGSMAAGGERSAFGGTQIVFGLLSVVVAGGLLLQAGQAVRGAWAVGQ
                     DRVSPAYPVVAAADRGSAFRVLWLGRVGGGSFPAPGGPADGVVEARRASVRYAVTGRS
                     GRSILSVGLPPGGPAYDELERALRTMLAGRIRHGGSLLAPFAVAYVVAGEGDLPPEAR
                     LRLDAQLDLLPGA"
BASE COUNT          925 a         2645 c         2551 g          968 t
ORIGIN      
        1 ggcgatcacg agatcgccgc gacgcgccac cacccgctgg gcctcccgcc ccacccagtc
       61 ctcgccttcg ggtacgagcc gcccctcggc cttcgagacg accttctgcg tcacggccac
      121 gacgtccccg tccttcagct ccgccgcatc cgcgatcagg gccgcgagat cgtcgtcctc
      181 gccgatctcg gggagccccg cgaccgggag gatctccacg ctcatcccag gagctccagc
      241 gcgaagcgcg cgaggcgctc cgaagcctcg tcgtccacca tgatcgtgtc ggtgacgccc
      301 acgcggaggc tctcccgctc ctcgatcttc ggggccaggt cgcggtcccg ctcgtcgacc
      361 acccaggcgg ccagcaggcc ccggtacgct tccgccgcgc cggccgccga cacctcgagg
      421 cccatcgcgg gcatcagctt gtcggccatc ccctggagca cggtcccgcc tacgatcggc
      481 gagatcccga cgacgcggtc cttccgctcc cgaagggcct cgtggacccc gggcacggcc
      541 aggatcggca tgacgctcac cacagggttc gaggggcaga tcacgatcgc gtcggcctcg
      601 gcgatcgccg ccagcgtgcc cggcgccggc ctcgccttct cggccccctc gaaccgcacc
      661 cgctccacct tcggctcggc tcggtgcgcg acccagtaca tctggaacgg gagcgatccc
      721 ccctcgacgt cgatccaggt ccgcacggga tcctcggtca cggggagcac gcgacacggg
      781 accccgagcg cctcgcacag ctcggccgtc acctccgaga gcgtcgcgcc ctcgttcagc
      841 cgctgggtcc gatggaggtg tgttgccagg tcgcggtccc cgagcgagaa ccaggcctgc
      901 cccccgaggc gccccagctc ctgcatcgcg cggaacgact cctccgcccg cccccagcct
      961 cgctccctat ccatccgccc gctcagccag tacgcgaccg agtcgaggtc gggcgagacg
     1021 tgcagcccga gatgcaccat gtcgtccccg acgttgccga tcacggtcac gtcagggggg
     1081 tccacggccc ggacgaggcc tctgaggagc ttccccgcgc ccacccctcc tgccagcgcc
     1141 gtcacccgca tgcgcacagc ctacctttga ccgaaccgag ggccggggct acgctccggc
     1201 catggctccc atccccgcgg aggttcgccg ggccctggcc cgcgccgagg caggcaagtc
     1261 cctgtccctc ggagacgtga ccgcgctgct cgcggcccgg ggagaggagc tggaccgcct
     1321 cctcgcggtc gcctcccgcc tccgcgagct gggctggggc cgcacgatca cctactcgcc
     1381 caaggtcttc atcccgctca cgatgctgtg ccgggaccac tgccactact gcacgttcgc
     1441 caagcccccg gcgcggatcg aggccccgta cctcacgccc gagcaggccg tggagatcgc
     1501 cgaggccggc cgccgggccg gctgcaccga ggccctcttc accctcgggg atcgtcccga
     1561 ggaccgctac cccgaggccc gggcctggct cgatgagcga ggccacgcga gcacgctcga
     1621 ctacgtccgc gaggtggcga tccgggtgat cgaggagacc ggcctcctgc cgcatctcaa
     1681 ccccggggtc atgtcctggg aggagatggc ccgcctcaag cacgtctcgg tctcgatggg
     1741 gctgatgctg gaacaggcga gcgaccgcct cctggagaag ggcctgcccc accacggctc
     1801 gcccgacaag gtccccgccg ttcgcctccg gatgatcgag gaggccgggc ggctctcgat
     1861 cccgttcacc accgggatgc tcatcgggat cggggagacc atcgaggagc gcgcggagac
     1921 cctgcttgcg atccgggagg ccgagcgccg ctaccgccac gtccaggagg tgatcgtcca
     1981 gaacttccgg gccaagcccc gcaccaggat gcgggaccat cccgagcccg atcgcgacga
     2041 cctgctggcc gcgatcgcca ccgcccgcgt cgtcctcggg ccgcgggtca gcgtccaggc
     2101 cccgcccaac ctctccgacg agggcttccc ccggctgctc gacggcggcc tcaacgactg
     2161 gggcggggtc tccccggtca cccccgacca cgtgaacccc gagaggccct ggcccaagct
     2221 ggagcgcctc cgcgccgcga ccgaggcccg aggcttcgcc ctccgccccc gcctacccgt
     2281 ctacccgcgc tacgtgctca agcccgaccc ctggatctcg gggaagatgc gggcgccgct
     2341 ccaggccctg gccgggcccg acggctaccc cagcggccgc cgccccgagc ccatcccctg
     2401 gcaggacccc gacgtgcagt ggaagccgcg cgagatcgac ctcgcgttcg ccaaggcggc
     2461 cggggccggg ctgcgggagg acgccctcga cgtctacggc gacttcgagc agctcgacgt
     2521 cacccgccgg tgggcccagg agcgggtgac ccccgagcgg ctggacgcgg agatccgggg
     2581 ggcgctggga agggccgccg gcggcctccc catcaccgac gacgaggccc tggcgctgtt
     2641 ccgggccgag gggccggcgc tggacgccct ggcggaggtg gccgacgacc tgcgccgcga
     2701 gaccgtgggc gaggaggtca cctacgtcgt caaccggaac atcaacttca ccaacgtctg
     2761 ctacacgggg tgccgcttct gcgccttcgc ccagcgcgag atcgatcccg agtcctacac
     2821 gctcaccctt cacgaggtgg ccgaccgggc cgaggaggcg gcggcggccg gggccaccga
     2881 ggtctgcatg cagggcgggc tccaccccga cctccccggg accttctact tcgatctgct
     2941 ggacgcggtg aaggcccgcg tgcccgagat ccacgtccac gccttcagcc cgatggaggt
     3001 gctgaacgga gcgacgcgcc tgggcatctc gttccgggag ttcctccagg agtgccgccg
     3061 ccggggcctg gggtcgatcc cgggcaccgc tgccgagatc ctcgacgacg acgtgcggtg
     3121 gctgctgacg aaggggaagc tcccggccga cacgtgggag gagatcgtcc ggaccgcgca
     3181 cgaggtcggc ctgccctcca gctccaccat catgtacggg cacgtggacg caccgcccca
     3241 ctgggtggcg cacatccggc gcctcgccgg gctccaggac gacaccggcg gcttcaccga
     3301 gatcgtcccc ctgccgttcg tccaccagaa ctcgcccatc tacctggccg ggaaggcccg
     3361 gcccggtccc acccacgagg agaaccgccg gatgcacgcg atcgccagga tcctgctcca
     3421 cggcctgatc cccaacgtcc aggtctcgtg ggtgaagctc ggcctggagg cgtcgcaact
     3481 gatgctccgg ggcggggcca acgacctcgg ggggaccctg atggaggaga cgatctcgcg
     3541 gatggccggc gcggagtggg ggatccgcaa ggagcccgag gagctcgagc aggcgatccg
     3601 gaccatcggg cgcgtgcccg cccagcgcac cacgacgtac ggtcgcgccg ccgcccgtct
     3661 gtgagaaagg cggcggcggc gcttcacacg cctccggaaa aaagtcatca tagggcctgg
     3721 acaagaagag ggcgtcccgg tcagaattac atgcatgtga ttctggggag gggcgccgtg
     3781 caccactaca tcccatggca ggagcgagcg aggtgccggg aattcgaccc ggagatcttc
     3841 ttccccgaga agggagggtc ctcccgggag gcgaagcgga tctgcgccca gtgcgaggtg
     3901 cggatcgagt gcctgaaata cgcactgcgc cacgacgagc ggtacggcgt ctggggtggg
     3961 atgagcgaac gggagcggcg acgcctcaag cggatggcgt cctgatcggg agaggggggt
     4021 gaccgatgcc accggcgcgg aagaggtcct ccgccaggaa gaaggcgacc ggtaggaaga
     4081 ggtccagcgc gaggaaggcg cctgcgcgca agaagaccgc ggcggcccgc aggaagacgg
     4141 cggggcgcag gaaggccacc gcgaggagga agacgacggc ccgccggaag acggcggggc
     4201 gcaggaaggc caccgcgagg aggaagacgg cggcccgcaa gaaggccacg ggccgcagga
     4261 aggccaccgc gaggaggaag acggcggccc gcaagaagac ggcggcacgc cggaagtcgt
     4321 ccggacggaa gagcacgcgg cgccggtagg cgcgccgggc tcggggaggg ggccgcgagg
     4381 ccccttctcc ctttccggat ggtgctatgc tccgccgcgg tctcgggcca cccgcagccc
     4441 gcggccgcat ccatccctcg tgggatctgc agacgcccct agcgtgcttg cggtcctcgt
     4501 cgtcaaggac ggggccgact ggctgaagcg gtcgctggcc gcgctcgccc ggcagtccta
     4561 cgaccggctc ggcgtcctcg cggtggacaa cgcgtccacc gacggctcgg gggagctgct
     4621 gcgcgacctc ctgggccccc gccgggtggt gagcctccgc gagaacgtgg ggttcccggg
     4681 ggcggtggcc cgggccatgg agatccccgc ggcccgagag gcagacttcc tgctgctcct
     4741 ccacgacgac acggtgcttc accccgacgc cgtcgctcgc ctcgtggccg cggcggaggg
     4801 cggcgtcggg gtggtgggcc cgaaggttct cgacggcgag cgacgcgtgc tccgcgagat
     4861 cggctacacg atcgaccgat tcggctaccg ctactcggcg ctcgaggagg gtgagatcga
     4921 ccagggccag cacgactcgc cccgcgaggt gctgttcgtc tccggcacgg ccatgctggt
     4981 ggcccgcgac gcgtggctcc gggccggccc acccgacgcg cgcctccgtc cctcgcacgg
     5041 cgatctcgac ttctgctggc gggtccgcat cgccgggttc cgggtcgtga tggacccctc
     5101 ggccgtcgta taccacgagg cggcggggga cgccggcctc cgggagggcg caaggagcga
     5161 tcgcacccgg taccacgagg agcggtcggc cctgaccgcc atgctcaaga actaccgtct
     5221 gggcacgctt ctgtgggtca tgccgatgta cctggtgcag ggcgtggcca aggtggtgct
     5281 gttcctcctg acgcgtcggt tcgccgacgc ccgtcaggtg ctcggggcgt gggcgtggac
     5341 gctttggcgc ttcccgggga cggtgcggcg acgggtccgg ctccacgcgg tccgtgcggt
     5401 gaaggagcga gaggtcaccg ccacgatggc cccgacctcg gcccgcctgc ggcgttgggc
     5461 ggggcaggtc tcggcggtgc tcttcccggc gaggggcgcg agcgagctgg cgcccgagga
     5521 ggagatcgct ctcgtgcctc tgcggcagcg ggtcggcggg ttcctggggc gtaacccggc
     5581 ccccgtggcg ctggggatcg ggctcctcct gaccgtggtg gcgttccggg gggtcctgtt
     5641 cgcctcgccc ctcgagggcg gcgcgatccc caccttcccc gacagcgccc gagaggtgtt
     5701 cgccgcctac ctcgacggga ccgtccccgc tggcttcggc ggcgccgacg gcgccagccc
     5761 cgccctcgtc ccgctgggcc tcctgagcgt cctgaccctc ggaaaccccc ggaccctggc
     5821 gtggatcatc gtcgccctca cgccgctcct ggcggggttc gcggcgtacc gcgccgccct
     5881 gcgccggacc ggcgcccccg gggcggccgt ggcggcgggg gcctcctacg ccctctcggc
     5941 cgtcgtgctg tgggccgcct ccgagggccg ggtgggcgag atggccttcc tgatcggcct
     6001 cccctggatc ctcggccgca tcgccgatcc gttcgagcgg ccggtgggcc cgccgctgag
     6061 gtgggcgttg ggaaccgccg tcgtcctggc catcacgggg tcgttcttcc cggccgcgtg
     6121 ggcgccggtg gcgatcgcga cggtggtgac gctgatcgtc ggcgggcggc gggagcgccg
     6181 cctctcgggg gccgggatcg ccctcgccac gctcgtcatg gcggccgtgc tcgccttccc
     6241 cttcgtgctc accctggctc gggtgggggt gggccggagc gccgagctcg ggatcgccgc
     6301 ggagttcggg tcgatcctga ggctctcgcc cggggaggcc cctggctcgt ggctgcccgc
     6361 tctcttcctc ccggtgggcg gcgtcctcgg cttcgccctc gccacgcagc gtgccgctgc
     6421 gtggcgaggg ctcgtctcgg cgagtctcgc cctcccgctc gcctggctgg cggcggccgg
     6481 gtacctgccg agcgcggcgg ccgagcccat cgcgtacctt gggctcgccg cgttcacgct
     6541 gagtgcgctc gtgggcgtgg ggttcggctc gatggccgcg gggggcgagc gatcggcgtt
     6601 cggcggcacg cagatcgtct tcgggctcct ctcggtcgtg gtggccgggg gcctcctcct
     6661 ccaggccggg caggcggtgc ggggggcctg ggcggtgggg caggaccgcg tgagccccgc
     6721 ctatccggtc gtggccgccg ccgaccgggg atccgcgttc cgagtcctct ggctcggacg
     6781 agtgggcggg ggctcgttcc ctgcgcccgg ggggcccgcc gacggggtgg tcgaggctcg
     6841 gagggcctcg gtgcggtacg cggtcaccgg ccgctcgggg cgctcgatcc tctcggtggg
     6901 gcttcccccc ggagggccgg catatgacga gctggagcgc gcgctccgca cgatgctcgc
     6961 cggccgcatc cgccacggcg gctcgctgct cgcgcctttc gccgtggcct acgtggtggc
     7021 gggcgagggc gacctcccgc ccgaggcccg gctcaggctg gacgcgcagc tcgacctgct
     7081 gccgggggc
//
LOCUS       DASUTW010000245         7475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_856046, whole genome shotgun sequence.
ACCESSION   DASUTW010000245 DASUTW010000000
VERSION     DASUTW010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7475
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856046"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /gene="sepH"
                     /locus_tag="VF058_08410"
     CDS             complement(<1..1260)
                     /gene="sepH"
                     /locus_tag="VF058_08410"
                     /inference="COORDINATES: protein motif:HMM:NF040712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HEX6208363.1"
                     /translation="MKRIYLVGVSDDNKRLQLAQGKGARFGSMELPISPKLRALLREV
                     EADRLAKRGRKPPEEELKEEKARSRKAEAEAKQAREGLEAASRAAREAEKRAREAEKR
                     AAQAERRAQRAEAEATEARRRAEEAEKKAEAAEKRAGEAADRVREQTLKPDGSPAKGG
                     LAPGGFERATGGMEGVHAIIRPTSEATPRAAPRPKAPARRRAPARATGEKPAAERPKE
                     ERPAEELPKKEAAPRRKSPPMPEPKLTPAEIQTLLRSGRGIRSVAKQAEAPVDWVRRL
                     AAPIESERLGVVTQLMQSYVVRARLGRSAVPIGTAIVENLRDRGVRFPERVAEDGWTA
                     WRPDNREWRVRFTYESRGRKQRADWRFEPQSKTVVPENDLAGELAYRAPADGDGVGGA
                     GAALRPQRVSGGSRAAPTPPTKKAASRK"
     gene            complement(1340..1570)
                     /locus_tag="VF058_08415"
     CDS             complement(1340..1570)
                     /locus_tag="VF058_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208364.1"
                     /translation="MRIDCERCPYRRGSLRCADCLVEFVLRLEDGDVVVEADEETDGT
                     VELDADEARAIRVLSDAGLIEPVRIDPLEDAG"
     gene            complement(1645..2784)
                     /locus_tag="VF058_08420"
     CDS             complement(1645..2784)
                     /locus_tag="VF058_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX6208365.1"
                     /translation="MSGPLEGIRVLDLTRLLPGNHCTLLLADLGADVVKVEEPGRGDY
                     VRWFEPLVDGVSAIHRAVNRGKRSVALDLKRPEGVDAFRRLAAGTDALIESFRPGVMD
                     RLGVGYEALSVENPRLVWCSISGFGQDGPYRDRVGHDLNYLGYAGVLHAAGPPGGVPW
                     PPPVQPSDLAAGMAGALGVVASLMDVARTGRGRFVDASMLDAAVSWGVMGMAVLLGTG
                     SPPPRGGTMLTGGLACYRVYRCAGGGLLSVAALEERFWRNLCRALEAPDLLEGHLDPA
                     EQGRLTARLEEIFATQPRDEWVERLAGLETCVGPVLDMAEAMEDPQVRHRGLAEVDGE
                     VVGPGPTLRVSGAEADLRPAPGLGEHTEEVLRETGLSDEEIAALR"
     gene            complement(2781..2984)
                     /locus_tag="VF058_08425"
     CDS             complement(2781..2984)
                     /locus_tag="VF058_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208366.1"
                     /translation="MATRFRCISCGNLTRFDVVEVRRTRAYYHFALSGELQVEDEEVL
                     ASERESVTCRWCGSTEVEEVEVA"
     gene            3058..3993
                     /locus_tag="VF058_08430"
     CDS             3058..3993
                     /locus_tag="VF058_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020518192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6208367.1"
                     /translation="MASHRARSDGDTLVRSAGLTKIYGEVTAVRDLDLVVGRGEVYGF
                     LGPNGAGKTTTLRMLLGLIHPTSGSAEVLGRPPGDPDGLLRVGAMVEEPAFYPYLTGR
                     DNLRVVTRYAGLPEDGIEDALRTVELLPRADSKFKTYSQGMKQRLGIAAALLKDPDLM
                     ILDEPTNGLDPAGMVEMRTLIRRLGTGERTVLLSSHLLAEVQQICDRVGVISKGRLVF
                     EGSVDELRGRAAVAIRAEPLDLARRRAEEVLGPDRVEVRDGELIVDADPGEVPNLNRE
                     LVLADVQVSELRPASRSLEEAFLQLTGEGGEDDEG"
     gene            4007..4828
                     /locus_tag="VF058_08435"
                     /pseudo
     CDS             4007..4828
                     /locus_tag="VF058_08435"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            4923..6533
                     /locus_tag="VF058_08440"
     CDS             4923..6533
                     /locus_tag="VF058_08440"
                     /inference="COORDINATES: protein motif:HMM:NF005907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HEX6208368.1"
                     /translation="MRVVQSSFEDLGAPLSQVTFCFVDLETTGGSPADSRITEIGAVT
                     YEGGERLGSFQTLVDPLVPIPRFITHLTGIDDLEVRGAPPIEAALPSFLEFARGTILV
                     AHNASFDYRFLCAEADRLEYERPPGPPVCTAKLARRVLRNEVPNARLATLADYFRTAT
                     RPQHRALADAEACAEVFHGLLELGGRLGILTLSDLREAMRARGTPHWRKAPMADGLPN
                     GPGVYLFRSKDGGVLYVGKAKDVRARVRSYFYGDDRKRIDDLLASAEGVEAIPCRTEV
                     EALVLEARLIRQHSPPYNRRGKAWRRACYLKLDLAEAWPRLKVVRKVSGDGAYLGPFR
                     SSKQATLAKEALEEVVPIRRCTRSMGRSTRFSPCALADLGRCVAPCDGRVAPERYEEL
                     VRSLHSFLLAPGELLAALEDRMEALAAQERFEEAALARDRLRALVTALARSRIERWLV
                     NAGRLEVESEEGLRLAFRKGALVRRGDERGFTLPVPLDAAQEVRAATSWLAKARLRLV
                     DAEEAPREPVDGGAAIARLARRLATASR"
     gene            6557..>7475
                     /locus_tag="VF058_08445"
     CDS             6557..>7475
                     /locus_tag="VF058_08445"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX6208369.1"
                     /translation="MEQAVIVEGARTPIGKFLGSFSEVPTVDLGIAATQEAIRRAGIE
                     PDDVEELIFGHARQAGNGPNTARQVSYRSGIPQEVPAYNVNMACGSGTKAVQLAAQEI
                     MLGHREVVVAGGMENMTRTPALLLDFRFGYRMGDVPVKDGMYKDGFLDPLCGLVMGET
                     AENLAEKYEIPREEQDEFALRTQQKAAAATERLAKEIVPVEAPGKKGETVTVTEDEHP
                     RPETTMEGLAKLPAVFRQGGSVTPGNASGVTDGAAAMVLMSERRAKAEGREILARIVG
                     TSSAGVDPAYMGIAVVPAVRRLEDSTGLSL"
BASE COUNT          985 a         2846 c         2583 g         1061 t
ORIGIN      
        1 cttcctggag gccgccttct tcgtgggggg cgtcggcgcg gccctgctcc cgcccgacac
       61 tcgctggggc cggagggccg cgccggcgcc ccccaccccg tcgccgtcgg ccggcgcccg
      121 gtacgccaac tctccggcca ggtcgttctc cgggaccacg gtctttgact gcggctcgaa
      181 ccgccagtcg gcccgctgtt tcctcccacg ggactcgtag gtgaaccgga cccgccactc
      241 acggttgtcg gggcgccacg ccgtccaccc gtcctcggcg acccgctccg ggaaccgcac
      301 gccgcggtcc cgcaggttct ccacgatcgc cgtcccgatc ggcacggccg acctccccag
      361 ccgggcccgc acgacgtagg actgcatcag ctgcgtcacc accccgagcc gctccgactc
      421 gatcggggcc gccagccgcc ggacccagtc caccggggcc tcggcctgct tggccaccga
      481 gcggatcccc cgtccggatc gcaggagggt ctggatctcg gccggggtga gcttcggctc
      541 gggcatgggc ggggacttcc tacggggggc cgcctccttc ttggggagct cctccgctgg
      601 gcgctcctcc ttggggcgct ccgcggcggg cttctcgccg gtcgcgcggg ccggagcgcg
      661 tcggcgcgcg ggagccttgg gcctgggcgc cgcgcgcggg gtcgcctccg acgtgggccg
      721 gatgatggcg tggaccccct ccatgccccc ggtggcgcgc tcgaagccgc cgggagccag
      781 gccccccttc gccggcgacc catcgggctt cagggtctgc tcacggaccc ggtccgccgc
      841 ctccccggcg cgcttctccg cggcctccgc cttcttctca gcctcctcgg cccgccgccg
      901 cgcctcggta gcctcggctt cggcccgctg ggcccgcctc tcagcctggg cggcacgctt
      961 ctcggcttcc cgagcgcgct tctcggcttc ccgggccgcc cgggaggccg cctccagccc
     1021 ctcgcgggcc tgcttggcct cggcctcggc cttccgagag cgggccttct cctccttcag
     1081 ctcctcctcg ggcggcttcc tgccccgctt ggccagccgg tcggcctcga cctcacgaag
     1141 gagggcgcgg agcttggggc tgatggggag ctccatgctc ccgaacctcg cccccttgcc
     1201 ctgcgccagc tggagccgct tgttgtcgtc ggacacgccg acgaggtaga tccgcttcaa
     1261 ggcatcccct gacctggccg ttcagggcat tgtggcccct caccaggagc agggcaacga
     1321 acccccggcg gggcacccct cagccggcgt cctcgagcgg gtcgatcctg accggctcga
     1381 tcaagcccgc gtcggacaga acgcggatcg cccgggcctc gtccgcgtcc agctcgaccg
     1441 tcccgtccgt ttcctcatcg gcctcgacga cgacgtcgcc gtcctcgagg cgcaggacga
     1501 actcgaccag gcagtcggcg cagcgcaggc tgccgcgccg gtaggggcat cgctcgcaat
     1561 cgatccgcac ggcgtctcct ctcgggctcg ctgtcgtcgg ggccggtcga gcaccacggt
     1621 agcggccccc tctgacacga acgctcagcg cagggccgcg atctcctcgt cgctgaggcc
     1681 ggtctcccgc aggacctcct cggtgtgctc cccgaggccg ggcgccggcc tgagatccgc
     1741 ctccgctccc gagacccgca gcgtgggccc cgggccgacg acctcaccgt ccacctcggc
     1801 caggcctcgg tgccggacct gcgggtcctc catcgcctcc gccatgtcga gcaccggacc
     1861 gacgcaggtc tcgaggcccg cgagccgctc cacccactcg tcccggggct gcgtcgcgaa
     1921 gatctcctcc aggcgggccg tcaaccgacc ctgctccgcg gggtcgaggt gcccctcgag
     1981 cagatccggc gcctccagcg cccggcacag gttccgccag aagcgctcct cgagcgcggc
     2041 caccgacagc aggccgccgc ccgcgcaccg gtacacgcgg tagcaggcca gcccgcccgt
     2101 cagcatggtc cctccgcggg gcggcgggct ccccgtcccc agcagcacgg ccatgcccat
     2161 cacgccccac gacaccgcgg cgtccagcat cgaggcgtcc acgaaccgcc ccctccccgt
     2221 tcgcgccacg tccatcagcg aggccacgac gcccagcgcc cccgccatcc ccgcggccag
     2281 gtccgagggc tggaccggcg gtggccacgg cacgccgccc ggcggccccg ccgcgtgcag
     2341 gacccccgcg tagccgaggt agttcagatc gtggcccacg cggtcgcggt acggcccgtc
     2401 ctggccgaac ccgctgatcg agcaccagac gagccgcggg ttctccaccg agagcgcctc
     2461 gtagccgaca ccgagacggt ccatcacccc gggccggaac gactcgatca gcgcgtccgt
     2521 ccccgccgcc agccgccgga acgcgtccac cccttccggc cgcttcaggt cgagcgccac
     2581 cgaccgcttc ccgcggttca ccgctcgatg gatcgcgctc actccgtcca cgagcggctc
     2641 gaaccagcgc acatagtccc ctcggcccgg ctcctcgacc ttcaccacgt ccgcccccag
     2701 atcggccagc agcagcgtgc agtggttgcc cgggagcagc cgggtcaggt cgaggacgcg
     2761 gatcccctcg agtggcccgc tcacgccacc tcgacctcct ccacctcggt cgagccgcac
     2821 cagcggcacg tcaccgactc gcgctccgag gccagcacct cctcgtcctc gacctggagc
     2881 tcgcccgata gcgcgaagtg gtagtaggcg cgcgtccgcc gcacctcgac gacgtcgaag
     2941 cgcgtgaggt tgccgcacga gatgcagcgg aatcgcgtcg ccatggcggg ggccatcgta
     3001 ccggagcgat cctccggctt ggatggggcg cgctggcgag gggtaggctc ggggccgatg
     3061 gcctcccacc gggcacgctc ggatggcgac accctggtcc gctccgccgg gctgacgaag
     3121 atctatgggg aggtgaccgc ggtccgcgac ctcgacctcg tcgtcgggcg cggcgaggtg
     3181 tatgggttcc tcggcccgaa cggcgcgggg aagaccacca cccttcggat gctgctgggg
     3241 ctgatccacc ccacctcggg ctccgccgag gtcctgggcc ggcccccggg cgatcccgac
     3301 gggctcctcc gcgtcggagc gatggtcgag gagcccgcct tctatcccta cctgacgggg
     3361 cgggataacc tccgcgtcgt cacccgatac gccgggctcc cggaggacgg gatcgaggac
     3421 gccctccgaa ccgtggagct gctgccccgg gccgacagta agttcaagac ctactcccag
     3481 ggcatgaagc agcggctcgg gatcgccgcc gcgctcctca aggacccgga cctcatgatc
     3541 ctggacgagc ccacgaacgg cctcgacccc gcggggatgg tcgagatgcg gacgctcatc
     3601 cgccgcttgg ggacggggga gcgaaccgtc ctcctctcca gccacctgtt ggccgaggtt
     3661 cagcagatct gcgaccgcgt gggcgtgatc tccaaggggc ggctcgtctt cgagggctcg
     3721 gtggacgagc tgcgcggccg cgccgcggtc gcgatccgcg cggagcccct cgacctggcc
     3781 cgccggcgcg cggaggaggt cctcggcccc gaccgcgtcg aggtccgcga cggcgagctc
     3841 atcgtggacg ccgatcccgg cgaggtcccg aacctgaacc gggagctcgt cctcgcggac
     3901 gtgcaggtgt ccgagctccg gcccgcatcc agatccctgg aagaggcctt cctccagctc
     3961 accggcgaag gaggcgagga cgacgaaggg tagcctcgcc gccgagctcc tcaaggtccg
     4021 caagcggccg gcgatgtgga tcctcgtcgg cgtctacttc gtgatgggcc tcttcttcgg
     4081 ctacgtggtc ccctacctaa tcttcaagac cggaggcggc cccgagctcg agggcgtgcc
     4141 cctggagcag atcctgaatg aggcgctgcc gcacaacctc gtcccaagcg tggtgggcgg
     4201 cttccccctg ttcggcgggg cgatcgcgct gatcatcggg gcgctgatcg ccagcagcga
     4261 gtacggctgg gggacgctca agacgatcct cgtccagcgc ccgaggcggg tcgaggtcct
     4321 ggcggggaag ttcctggcga cggccctcgt cacgctggcg atcaccgtcg tcttcttcct
     4381 cctgagcgcg ctggccagct ggaccatcgc cttggccgag aacagggccg tctcgtggcc
     4441 cgggatcgtc gagctgctgc agggggtcgg ggctggctgg ctgatcctcg gcatgtggag
     4501 cctgttcggc atgacgctcg ggatcctgtt ccgaagcacc gcgctggcga tcggcctcgg
     4561 cctcgtctgg gcgctcgtga tcgagagcct gatcagcggc ttcgccaacc tgctcgaggt
     4621 cctggcgacg atccagcagt ggctgcccgg cgtgaacgcg ggcgcgctgg cggcgtcgct
     4681 cggggctcgg gcccaggatc aggcgggcgg cgtcccgggg gtgaccacgg tggccgacgg
     4741 gcccgaggcc acggcgctcc tcgtcgccta cgtggcgggg atggcgctcc tctccacgct
     4801 cgtgctccgc cgccaggaca tctcgtagac ggatcctcct tccatatgct tgacctcgaa
     4861 cacatgttcg ggtatggtcc cgtctgcgcg cgcccatccg atcgattcga tcaggaggag
     4921 gcgtgcgggt cgtccagtcg tcgttcgagg acctcggggc tcccctctcc caggtcacgt
     4981 tctgcttcgt ggacctcgag acgacgggcg gctcgcccgc cgactcccgc atcaccgaga
     5041 tcggggcggt cacgtatgag ggcggcgagc ggctcggctc gttccagacc ctcgtggacc
     5101 cgctggtccc catcccccgg ttcatcacgc acctgaccgg gatcgacgac ctggaggtcc
     5161 ggggcgcgcc gccgatcgag gccgcgctcc cctcgttcct ggagttcgct cgcgggacga
     5221 tcctcgtcgc gcacaacgcg tccttcgact accggttcct ctgcgccgag gccgaccgcc
     5281 tcgagtacga gcgcccgccc gggccgcccg tctgcaccgc gaagctggcc cggcgcgtgc
     5341 tccggaacga ggtccccaac gcgaggctgg ccacgctcgc cgactacttc cgcaccgcga
     5401 cccgccccca gcaccgggcg ctcgccgacg ccgaggcctg cgccgaggtc ttccacggcc
     5461 tcctcgagct cggcgggcgc ctgggcatcc tcacgctctc tgacctccgg gaggcgatgc
     5521 gggcccgagg caccccgcac tggaggaagg ccccgatggc cgacggcctc cccaacgggc
     5581 cgggcgtgta cctgttccgg agcaaggacg gcggggttct ctacgtgggg aaggcgaagg
     5641 acgtgcgggc ccgcgtgcgc tcctacttct acggggacga ccgcaagcgg atcgacgacc
     5701 tcttggcgag cgccgagggg gtcgaggcga tcccctgccg gaccgaggtc gaggcgctgg
     5761 tgctcgaggc gaggctgatc cggcagcact cccctcccta caaccgaagg gggaaggcgt
     5821 ggcggcgggc ctgctacctg aagctcgacc tggccgaggc ctggccgaga ctcaaggtgg
     5881 tccggaaggt gagcggcgac ggcgcgtacc tggggccgtt ccgaagcagc aagcaggcca
     5941 cgctggccaa ggaggcgctc gaggaggtcg tcccgatccg ccgctgcacc cgctcgatgg
     6001 ggcgctccac ccggttctcg ccgtgcgcgc tcgcggacct cggccgctgc gtcgcgccct
     6061 gcgacgggcg ggtcgccccc gagcgctacg aggagctcgt tcggtcgctg cactccttcc
     6121 tcctcgcacc gggcgagctc ctcgcggcgc tcgaggatcg gatggaggcg ctcgcggccc
     6181 aggagcggtt cgaggaggcc gcgctggcgc gggaccgcct ccgggcgctc gtcacggcgc
     6241 tggccaggtc gagaatcgag cggtggctgg tgaacgcggg gcggctggag gtcgagtccg
     6301 aggagggcct gcgcctggcc ttccgaaagg gagcgctcgt ccggcgaggc gacgagcgag
     6361 ggttcacgct gcccgtgccg ctggacgcgg cccaggaggt ccgcgcggcg acgagctggc
     6421 tggcgaaggc ccggctcagg ctggtcgacg ccgaggaggc gccgcgggag cccgtcgacg
     6481 ggggcgccgc catcgccagg cttgctcggc gtctcgccac ggcctcgcgg tagaggccgc
     6541 tggtaggatg ggcgccgtgg agcaggccgt catcgtcgag ggcgcgcgga cgccgatcgg
     6601 gaagttcctc gggtccttct ccgaggtccc caccgtcgac ctcggcatcg ccgccacgca
     6661 agaggcgatc cggcgagccg gcatcgagcc tgatgacgtc gaggagctga tcttcgggca
     6721 cgcccgccag gccgggaacg gccccaacac ggcccgccag gtctcctacc gcagcggcat
     6781 cccccaagag gtccccgcct acaacgtcaa catggcctgc ggatccggca cgaaggcggt
     6841 ccagctcgcg gcgcaggaga tcatgctcgg ccaccgagag gtcgtcgtgg ccggcggcat
     6901 ggagaacatg acgcgcacgc cggccctcct cctcgacttc cgcttcggct atcgcatggg
     6961 cgatgtgccg gtgaaggacg ggatgtacaa ggacgggttc ctcgacccgc tgtgcggcct
     7021 ggtgatgggc gagacggcgg agaatctcgc cgagaagtac gagatccctc gcgaggagca
     7081 ggacgagttc gcgctgcgaa cccagcagaa agccgcggcc gccaccgagc ggctggccaa
     7141 ggagatcgtc cccgtcgagg cacccgggaa gaagggcgag accgtcaccg tcacggagga
     7201 cgagcacccg cggcccgaga ccacgatgga gggccttgcc aagctccccg ccgtcttccg
     7261 ccagggcggc agcgtgaccc ccggcaacgc cagcggggtg accgacgggg ccgcggcgat
     7321 ggtcctgatg tccgagcgcc gggccaaagc cgagggccgc gagatactgg cccggatcgt
     7381 ggggacctcg tccgcaggcg tcgaccccgc ctacatgggc atcgccgtcg tgccggccgt
     7441 ccggaggctc gaggactcga ccggcctctc cctcc
//
LOCUS       DASUTW010000246         3919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_206257, whole genome shotgun sequence.
ACCESSION   DASUTW010000246 DASUTW010000000
VERSION     DASUTW010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206257"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1980)
                     /locus_tag="VF058_08450"
     CDS             complement(79..1980)
                     /locus_tag="VF058_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX6208370.1"
                     /translation="MLWLAPLAPLAAAGLAAVVRAGWGPWVAGGGVVVSLVVALAGAA
                     GELTASWGWGPALGLSLSADGIARVVVVLVPAVALPVLAYSASDHRGDPGRRRLLSLL
                     ALFVGAMQLVVLAGDLLTLLIGWELVAACSWALIGHRWREDAPRAGQEAFVVTRVGGV
                     GLVVAAGAALHGTGSLRFESLGAAGEWDLHLIAAGVLAAALAKSAQPPFSPWLFSAMA
                     GPTSVSALLHSATMVAAGAYALARLAPALEPTGWFGPAVIGFGLAGALLGGIVSLLQS
                     DVKRALAASTTAQYGLVLIAVGVLAVPAAAGHLAAHAVAKASLFLAAGVAIHAAGSGD
                     LARMRLGRALPAIAALFAVGAASLAGVFPLGGAFTKDAVIGAAWEYATWAGVGALLAG
                     ALSALYAGRIALLAYGPGEARRPAAPPALQRAAIAVLTLLVVLLGLLWVPPLREGLAG
                     IVGADAVAPAAWEVLLSLGAVLLAAVVVLLLDRGRALASLGLPDRLRVPAADWLGLPA
                     AGRALVVTPVLALSRGLARVDDRVVDAGVRAVGRVADLFARAFRAWGERGMDGVVEGV
                     GRGTLASADGSRRVDERVVDGSVEGMARGVGAAGAASRLLQSGLAHHSYVIIAVGLAV
                     AAAVAFLVR"
     gene            complement(1980..2285)
                     /gene="nuoK"
                     /locus_tag="VF058_08455"
     CDS             complement(1980..2285)
                     /gene="nuoK"
                     /locus_tag="VF058_08455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006911983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX6208371.1"
                     /translation="MSQELLLVVAAALFGIGVYGALSQQTFVMIMMGLELMIAGGILA
                     IGTFWAQSAAGSPKGQVLAIVAMTVMAVEAALGFALVIAVYRVRKADMTEKLKRLKG"
     gene            complement(2282..2833)
                     /locus_tag="VF058_08460"
     CDS             complement(2282..2833)
                     /locus_tag="VF058_08460"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007068915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX6208372.1"
                     /translation="MIVDVLSWVFGVGSVLCAWRVFRADSMVRAAYWLLAAFVFVGLL
                     LVLLTAQFLGLVLILMMAGEMTIMAVFMVMFMMNPAGLNPMTMVHQHRTSIAAGVVAF
                     LGLAAVGLLGSFPERAVAAGARVTRETGMELLGDSMVVFETAAVALLATMIGTVALAS
                     RRGRAGDAYEGSVPPSMDPEEAK"
     gene            complement(2830..3759)
                     /locus_tag="VF058_08465"
     CDS             complement(2830..3759)
                     /locus_tag="VF058_08465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX6208373.1"
                     /translation="MRSAAAVLATMAAMALGVYVAAALDAMAGAAVGGRRSVGRSLTF
                     PMRRGLALLLRPRARTERPDAEAWALAPAALAGLAAVGLATIPLAPGLAVADVSAGIV
                     LFGAAMALVMVAVFLQGWGPNSLHPLIAGYRFAGQALSYEMPLALVLIGAALPAESLS
                     VGAIVESQAHLWNALRQPLGMPLFLVAVAGLAFWGPLALPEGEDLAGGTSAEVSGAAR
                     LTWKAARLMVLVAGAAMTAAVFLGGWQGPWIPGWAWTILKTLAVLVLLVWARHRFVRV
                     PVERFVWVSWVVLIPLALVDVFLAGAQALWAAP"
BASE COUNT          648 a         1601 c         1350 g          320 t
ORIGIN      
        1 tcgcggctcg cgggcagcag cgcgatcgcg atcgccgccg ccagcggcac gaagatggca
       61 gccgtcagca tgctccgctc atctcaccag gaaggcgacc gcggccgcca ccgcgagccc
      121 gacggcgatg atcacgtagg agtggtgggc cagccccgac tggagcagtc ggctcgcggc
      181 cccggccgcc cccacacccc gcgccatccc ctcgaccgag ccgtccacga cgcgctcgtc
      241 cacccgacgg gagccgtccg ccgacgccag cgtccccctc ccgacgccct ccaccacgcc
      301 gtccatgcct cgctcccccc acgcgcggaa ggcccgggcg aagaggtcgg cgacccgccc
      361 caccgcccgg acgccggcgt ccacgacgcg atcgtcgacc cgggccagcc cgcgggagag
      421 ggccaggacg ggcgtcacca ccagggctcg acccgctgcg gggagcccca gccagtcggc
      481 ggccgggacc cgcagccggt ccggcagccc gagcgacgcc agcgcgcggc cgcggtcgag
      541 gaggaggacg accaccgccg cgagcaggac cgctcccagc gagagcagga cctcccacgc
      601 cgcgggggcg accgcgtcgg cccccacgat cccggccagg ccctccctga ggggcgggac
      661 ccagaggagg ccgaggagca cgacgagcag ggtcaggacg gcgatcgcgg cccgctggag
      721 cgcggggggg gctgctggcc ggcgcgcctc ccccgggccg taggcgagca gggcgatcct
      781 ccccgcgtag agcgcggaga gcgcgccggc cagcagcgcc cccacccccg cccacgtcgc
      841 gtactcccac gccgccccga tcaccgcgtc cttcgtgaac gcgccgccca gcgggaaaac
      901 gccggccagc gaggccgccc ccaccgcgaa cagcgcggcg atcgcgggca gggcccggcc
      961 gaggcgcatc ctcgcgagat cgcccgagcc cgcggcatgg atcgccacgc cggccgccag
     1021 gaacagcgac gccttggcca cggcgtgcgc cgccaggtgc ccggcggccg cgggcacggc
     1081 gagcaccccc accgcgatca gcacgagccc gtactgcgca gtcgtggagg cggcgagcgc
     1141 ccgcttcacg tccgactgga gcagggacac gatgcccccg agcagcgcac cggccagccc
     1201 gaagccgatc accgcgggac cgaaccatcc ggtcggctcc agcgccgggg ccagacgggc
     1261 cagggcgtag gcgccggcgg ccaccatcgt ggcggagtgg agcagggccg agaccgacgt
     1321 ggggccggcc atcgccgaga agagccacgg cgagaacggc ggctgggccg acttggccag
     1381 ggccgcggcc aggacgcccg ccgcgatcag gtgcaggtcc cactcgcccg ccgcgcccag
     1441 ggactcgaag cgcagggagc ccgtcccgtg gagcgcggcc cccgccgcaa ccacgagccc
     1501 cacgcccccc acccgggtca ccacgaaggc ctcctggccc gcccggggcg cgtcctcacg
     1561 ccaccggtgc ccgatcaggg cccaggagca cgcggccacg agctcccacc cgatcagcag
     1621 cgtgaggagg tcgcccgcga gcacgaccag ctgcatcgcc ccgacgaaca gggccaggag
     1681 cgagaggaga cggcgccggc ccggatcccc ccgatgatcc gacgccgaat acgccagcac
     1741 cggcagcgcc acggccggaa ccaggacgac cacgacgcgg gcgatcccgt ccgccgacag
     1801 cgagaggccg agcgcgggcc cccaccccca cgacgcggtc aactcccccg ccgcgcccgc
     1861 cagcgccacg accagggaga ccacgacccc gccgccggcg acccaggggc cccatccggc
     1921 ccggaccacg gcggccaggc ccgcggcggc gagcggcgcg agaggggcga gccagagcat
     1981 cagcccttca gcctcttcag cttctcggtc atgtcggctt tccgaacgcg gtagacggcg
     2041 atcacgagcg cgaaccccag cgcggcctcc accgccatca cggtcatggc gacgatcgcc
     2101 aggacctgtc ccttgggcga gcccgctgcg ctctgcgccc agaaggtccc gatcgcaagg
     2161 atgccgcccg cgatcatcag ctcgaggccc atcatgatca tcacgaacgt ctgctggctg
     2221 agcgccccgt agacgccgat cccgaacagc gcggccgcca cgacgagcag gagctcctgg
     2281 ctcacttcgc ctcctccggg tccatcgagg gaggcaccga gccctcgtac gcgtcgccag
     2341 ccctcccccg tcgcgacgcc agcgccaccg tcccgatcat cgtggccagg agggccacgg
     2401 cggccgtctc gaagaccacc atcgagtccc cgagcagctc catccccgtc tcgcgggtca
     2461 cgcgggctcc cgccgccacc gcccgctccg ggaaggagcc caggagcccc accgcggcca
     2521 gcccgaggaa cgccaccacc cccgccgcga tcgaggtccg gtgctgatgg accatcgtca
     2581 tggggttcag cccggccggg ttcatcatga acatgaccat gaagacggcc atgatcgtca
     2641 tctcgccggc catcatcagg atcaggacga gccccaggaa ctgggccgtc agcaggacca
     2701 gcaggagccc gacgaagacg aacgcggcca gcagccagta ggcggcccgc accatcgagt
     2761 cggcccggaa cacgcgccac gcgcacagca ccgagccgac gccgaagacc caggagagga
     2821 cgtcgacgat cacggcgccg cccacagagc ctgggccccg gccaggaaga cgtccacgag
     2881 cgccagcggg atcagcacga cccacgagac ccacacgaac cgctcgaccg gcacgcggac
     2941 gaagcggtga cgagcccaca ccagcaggac cagcacggcg agcgtcttga ggatcgtcca
     3001 ggcccacccg gggatccacg ggccctgcca tcccccgagg aagacggcgg ccgtcatcgc
     3061 cgcccccgcc acgagcacca tcagccgggc cgccttccag gtcagacgcg ccgcgccgga
     3121 gacctcggcc gaggtgccgc cggcgagatc ctccccctcg ggcagggcca gcgggcccca
     3181 gaacgccagc cccgccaccg ccacgaggaa cagcggcatc ccgagcggct ggcgaagcgc
     3241 gttccacagg tgggcctggg actcgacgat cgcgccgacc gagagcgact cggccggcag
     3301 ggcggctccg atcagcacca gcgccagggg catctcgtag gagagcgcct gcccggcgaa
     3361 gcggtagccc gcgatgaggg gatggaggga gttcgggccc catccctgga ggaacaccgc
     3421 caccatgacg agcgccatcg cggccccgaa cagcacgatg cccgcgctca cgtcggccac
     3481 cgccagcccg ggcgccagcg ggatcgtggc cagcccgacg gcggccaggc cggccagggc
     3541 ggcgggggcg agcgcccagg cctcggcgtc ggggcgctcg gtcctggcgc gggggcggag
     3601 gaggagcgcg agcccccgcc gcatggggaa cgtcagcgac cgccccacgc tgcggcggcc
     3661 gccgaccgcc gcgccggcca tcgcgtccag cgccgccgcc acgtagacgc cgagcgccat
     3721 cgccgccatc gtcgccagca cggcggcggc ggacctcacg ccgggacctc cctcagtgcc
     3781 gcgccctctt cgaggtcgag gtcgaggctc accacggtcg tcacgagatc gccccactcc
     3841 atgcccgtca ggaggcccgg caggaggtcg aggagcgcgc cggccccgag tccgggcgct
     3901 ccgatcggcc cgcggggcc
//
LOCUS       DASUTW010000247         4317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1373705, whole genome shotgun sequence.
ACCESSION   DASUTW010000247 DASUTW010000000
VERSION     DASUTW010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373705"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="VF058_08470"
     CDS             complement(<1..787)
                     /locus_tag="VF058_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6208374.1"
                     /translation="MRVGVQLPEVEREVRWPEYVAMARAAEEVGFDSLWVGDHLLYRD
                     DGRPERGPWDAWTLLAGLAAVTERVRLGPLVACTAFRPPGVLARTAAAVHELSGGRLV
                     LGLGAGWNEEEFRAFGIPFDHRASRFEESFEIVRRLLAGERVTFRGRFHEAQDAVLLP
                     RPTDRIPLMIGSTGMRVLAGALPHVDIWNTWYDWYGNTPEGFARGNAEIDRAVERVGR
                     EPDAVERSACVLVVVDEGAGERPAPEGVRPLTGGPEEVGAGRRQ"
     gene            811..1095
                     /locus_tag="VF058_08475"
     CDS             811..1095
                     /locus_tag="VF058_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208375.1"
                     /translation="MAEIACPSCGEEERLEGSRREGSVEIACLACGHRWERDTRRRCG
                     LCGSEDLRYTPEPLWERGRGEQRTPAGRRDAWACNACGGRDVTSAAPRPG"
     gene            complement(1096..1545)
                     /locus_tag="VF058_08480"
     CDS             complement(1096..1545)
                     /locus_tag="VF058_08480"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HEX6208376.1"
                     /translation="MAQARKQPGVERIYRDDHIEVTWEPSLCIHYGACYGQLPRVFRP
                     RERPWVRLEEASADEIGKVVQDCPTGALGFRRLDGGPQEKVPEETEIRLDPKGPIYLR
                     GRVRIEREGEVLREGTRVALCRCGQSGNKPFCDGTHLKIGFTDEPEA"
     gene            1634..2248
                     /locus_tag="VF058_08485"
     CDS             1634..2248
                     /locus_tag="VF058_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013287440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX6208377.1"
                     /translation="MGRAFVQGIVAGYGIAIPVGAIAVLIVDLALRRGFPAAAAAGAG
                     AASADLLYAALAMLAGSAIAAALEAVGERMRLLSAVVLLGIAVWLGARALREPAPAEA
                     SGPERGGLLRIFSGFLGLTLLNPVTIVYFTALILGLRGEGLEAGSAKVAFVIGAFLAS
                     LSWQWLLAGAGSVLRHRLPAGARRVTSLLGSGIVALLALRILLS"
     gene            complement(2255..2974)
                     /locus_tag="VF058_08490"
     CDS             complement(2255..2974)
                     /locus_tag="VF058_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007265183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX6208378.1"
                     /translation="MERREGYRAARRDSSLAVLEGFHALKHAIRFGAELLDVVASEDA
                     PLDELAADLAPDVAGRLRAVERIPAEGFAELTPTPPATGIAALARRPPVDVAAVLGDP
                     GPAPVVFLEKPRRLENVGAVVRVAAAASAAGVLSSGPHDPWHPAAVRGSAGLHFAVPV
                     ARVDALPASDRPLVALDPEGIELGPGVLPPRAVAAFGTERVGLGREILRRADLRVRIP
                     MREGVSSLNLATAVAVVLYTR"
     gene            3006..4007
                     /locus_tag="VF058_08495"
     CDS             3006..4007
                     /locus_tag="VF058_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEX6208379.1"
                     /translation="MEIATTDYHTAGEPFRIVTGGAPALEGPSILDKRAFAAERLDEV
                     RQLLIHEPRGHADMYGCFVTDPEDEDADLGVVFFHNAGYSTACGHGTIALVTWALESG
                     RLDPQGLEVEDGEVRVVVDVPSGRLPTWARLVGDRVRSVRFRNVPSFCGARGIEVAGV
                     TADVSYGGAFYASVGAADAGLTVEPEHTEGFIALGREIQAALNERGIGEHPAEERLSG
                     VYGVIFHEPAPEPEARVHQRNVAIFADGEVDRSPCGSGTSARLALLHADGGIGLGEWM
                     LHDGIVGTRFRGRIVEETGIHGLPAVVTEVEGSAHRSGEHRFVLEEDDPLGTGFLLR"
     gene            4012..>4317
                     /locus_tag="VF058_08500"
     CDS             4012..>4317
                     /locus_tag="VF058_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX6208380.1"
                     /translation="MAITIALAGDTMLGRGVAERIEERGPRSLVAPEVVSLMRRADLC
                     MLNLECCISERGEPWPAPGKPFFFRAPATAVETLVDLGVDAVTLANNHALDFGEVALL
                     "
BASE COUNT          520 a         1574 c         1634 g          589 t
ORIGIN      
        1 tctggcggcg gccggcgccg acctcctcgg gaccgccggt cagcggcctg acgccctccg
       61 gggcggggcg ctcgccggcc ccctcgtcca ccacgacgag cacgcacgcg ctccgctcca
      121 ccgcgtccgg ctcgcgcccc acccgctcca cggcccgatc gatctccgcg ttcccccggg
      181 cgaagccctc cggggtgttg ccgtaccagt cgtaccaggt gttccagatg tcgacgtggg
      241 ggagcgcgcc ggcaagcaca cgcatgcccg tgctcccgat catcagcggg atcctgtcgg
      301 tcggccgcgg gagcagcacc gcgtcctgcg cttcgtggaa gcgcccgcgg aacgtcaccc
      361 gctctcccgc cagcaggcgc cggacgatct cgaacgactc ctcgaacctg gacgcccgat
      421 ggtcgaaggg gatcccgaag gcccggaact cttcctcgtt ccaccccgcc cccagcccga
      481 gcacgagccg ccccccgctc agctcgtgga ccgcggccgc cgtgcgcgcc agcacgcccg
      541 gcggccggaa cgccgtgcac gccacgagcg gccccaggcg cacccgctcg gtgaccgcgg
      601 cgagccccgc gagcagggtc caggcgtccc acgggccgcg ctcgggccgc ccgtcgtccc
      661 ggtagaggag gtgatcgccg acccagagcg agtcgaagcc cacctcctcg gcggcgcgcg
      721 ccatcgccac gtactcgggc cagcgcacct cgcgctcgac ctctgggagc tggaccccga
      781 cgcgcatgga gtgagtctag tctgggggtc gtggccgaga tcgcgtgtcc ttcctgcggc
      841 gaggaggagc gactcgaggg atcccgtcgt gaggggtcgg tcgagatcgc gtgcctcgcc
      901 tgcgggcacc gctgggagcg ggacacgcgg cgccgctgcg ggctctgcgg atccgaggac
      961 ctccgctata cgccggagcc gctgtgggag cgggggcgcg gcgagcagcg gacgccggcc
     1021 ggccggcggg atgcgtgggc ctgcaacgcg tgcggcggga gggacgtcac gtcggccgcc
     1081 ccgcggccgg ggtgatcagg cctcgggctc gtcggtgaag ccgatcttga ggtgggtgcc
     1141 gtcgcagaac ggcttgttcc ccgactgtcc gcagcggcac agggcgacgc gggtcccttc
     1201 ccgcagcacc tcgccctcgc gctcgatccg aacccgcccc cggaggtaga tcgggccctt
     1261 cggatcgagt cggatctccg tctcctcggg caccttctcc tgggggccgc cgtcgagccg
     1321 ccggaacccc agcgcccccg tcggacagtc ctggacgacc ttcccgatct cgtccgcgct
     1381 cgcctcctcc aggcggaccc acggcctctc ccggggccgg aagaccctcg ggagctggcc
     1441 gtagcacgcc ccgtagtgga tgcagagcga cggctcccac gtgacctcga tgtggtcgtc
     1501 gcggtagatc cgctcgaccc ccggctgctt ccgtgcctgt gccaccgcgt cccctctcgt
     1561 cccttctgcg catggtaccg ggtgggggcc ggggcgtcgc tggtacgatg cccgccgtcg
     1621 agggaggggg ctcgtgggga gggcgttcgt ccaggggatc gtggcagggt acgggatcgc
     1681 gatccccgtg ggcgcgatcg cggtgctgat cgtggatctc gccctgcggc gggggttccc
     1741 ggccgcggcg gccgcgggcg cgggggcggc gtcggccgat ctcctctacg cggcgctggc
     1801 gatgctcgcg gggagcgcca tcgccgcggc actcgaggcg gtcggggagc ggatgcgtct
     1861 cctcagcgcc gtcgtcttgc tggggatcgc ggtgtggttg ggcgcgcggg cgctccggga
     1921 gccggcaccc gcggaggcct ccgggccgga gcggggtggt ctgctccgca tcttctccgg
     1981 gttcctcggg ctcaccctgc tcaacccggt cacgatcgtg tacttcaccg ccctgatcct
     2041 cggactgcgc ggcgaagggc tcgaggccgg atcggcgaag gtcgccttcg tcatcggcgc
     2101 cttcctcgcc tccctgtcct ggcagtggct gctggccggc gccgggtccg tcctccgcca
     2161 ccggctcccg gcgggggccc ggcgcgtcac gagcctcctc ggcagcggga tcgtcgccct
     2221 cctggcgctc cggatcctcc tgagctgatc cgggtcagcg cgtgtagagg acgacggcga
     2281 ccgccgtcgc gaggttgagg cttgagacgc cttcccgcat ggggatgcgc acccggagat
     2341 cggctcggcg caggatctcc cggcccaggc cgactcgctc ggtcccgaac gccgccacgg
     2401 cccggggcgg gaggacgccg ggacccagct cgatcccctc cgggtcgagc gcgacgagcg
     2461 gacggtcgga tgcgggcagg gcgtcgaccc gagccaccgg gacggcgaag tggagccccg
     2521 cgctcccacg gaccgccgcc ggatgccagg ggtcgtgcgg ccccgagctg aggactccgg
     2581 cggccgacgc cgccgcggcg acgcgcacca cggcgcccac gttctcgagg cggcgaggct
     2641 tctccaggaa gaccaccggt gcgggtcccg ggtcgcccag cacggccgcg acgtccaccg
     2701 gcggtctgcg ggccagcgcg gcgatgcccg tggccggagg cgtgggcgtg agctccgcga
     2761 agccctcggc ggggatccgc tcgacggctc gcagccgacc ggccacgtcc ggcgcgaggt
     2821 cggccgccag ctcgtcgagc ggggcgtcct ccgaggctac gacgtcgagg agctcggccc
     2881 cgaaccggat ggcgtgcttc agggcgtgga acccttccag cacggccagc gaggagtccc
     2941 gccgcgccgc ccggtacccc tccctccgct ccacgccgcg agtctcccac gcgtatcctg
     3001 cggccgtgga gatcgcgacc accgactacc acaccgcggg ggagcccttc cggatcgtga
     3061 cgggcggggc gccggccctc gagggaccct cgatcctcga caagcgggcc ttcgccgccg
     3121 agcgcctgga cgaggtccgg cagctgctga tccacgagcc ccgggggcac gcggacatgt
     3181 acgggtgctt cgtcaccgac cccgaggacg aggacgccga cctcggggtc gtcttcttcc
     3241 acaacgcggg ctacagcacc gcctgcggcc acggcacgat cgccctggtc acgtgggccc
     3301 tggagtcggg caggctcgac ccgcaggggc tggaggtcga ggacggcgag gtccgggtcg
     3361 tggtggacgt cccctcggga cggctcccga cctgggctcg gctcgtggga gaccgggtgc
     3421 gctcggtgcg cttccggaac gtgccgtcct tctgcggggc tcgggggatc gaggtggcgg
     3481 gggtcacggc agacgtctcg tacggcggag ccttctacgc ctcggtgggc gcggccgacg
     3541 cggggctcac cgtggagccc gagcacacgg aggggttcat cgccctgggc cgagagatcc
     3601 aggccgcgct gaacgagcgg gggatcgggg agcacccggc ggaggagcgt ctgagcggcg
     3661 tgtacggggt gatcttccac gagccggctc ccgagcccga ggcccgggtc catcagcgga
     3721 acgtggcgat cttcgccgac ggcgaggtgg accgctcgcc ctgcgggagc gggacctcgg
     3781 cccggctggc cctgctccac gccgacggtg ggatcgggct gggggagtgg atgctgcacg
     3841 acgggatcgt ggggacgcgg ttccggggac ggatcgtgga ggagacgggg atccacggcc
     3901 tccccgcggt ggtcaccgag gtggagggat ccgcgcaccg cagcggggag caccggttcg
     3961 tgctggagga ggacgacccg ctggggacgg gcttcctcct gcgctgaggc cgtggcgatc
     4021 acgatcgcgc tggccggcga cacgatgctc gggcgggggg tggccgagcg gatcgaggag
     4081 cgcgggccga ggtccctggt cgctcccgag gtggtctcgc tgatgcggag ggccgacctc
     4141 tgcatgctga acctggagtg ctgcatctcg gagcgtgggg agccgtggcc ggcgcccggg
     4201 aagccgttct tcttccgggc gccggccacg gcggtcgaga ccctggtgga cctgggcgtg
     4261 gacgccgtga cgctggccaa caaccatgcg ctcgacttcg gcgaggtggc cctgctc
//
LOCUS       DASUTW010000248         2688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_79607, whole genome shotgun sequence.
ACCESSION   DASUTW010000248 DASUTW010000000
VERSION     DASUTW010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79607"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VF058_08505"
     CDS             <1..220
                     /locus_tag="VF058_08505"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HEX6208381.1"
                     /translation="SVAAAESATVLVPFVPLLREGPVSDRLVTQDNVDQVPGEVSLVL
                     ALEDLVELGEAGHYGVKPGANGLVPSLG"
     gene            220..891
                     /locus_tag="VF058_08510"
     CDS             220..891
                     /locus_tag="VF058_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6208382.1"
                     /translation="MPALAFVAARDEESRVGETVRAIRDLPVDEIVVVDDGSRDGTAA
                     EALAAGARVLVAPRPMGKGEALEAALDRAPAADVYLFLDGDLGATASAAVPLLELVRD
                     GKADLAIAELPRQPGHGGFRVVKRLSARLIRSLSGFRAREPMSGQRAMTREVLEAVRP
                     LAPGFGVETAMTIDAVRAGFRVMEVEVPMSHRVTGRDLAGFAHRARQGRDVLAAAWPR
                     AIGRR"
     gene            888..1697
                     /locus_tag="VF058_08515"
     CDS             888..1697
                     /locus_tag="VF058_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208383.1"
                     /translation="MTWVSLAAGALVGAALAWFSQLRPPRGLVRENHAGLPVPAVLGL
                     ALVGGAVVGHAVGRPEAEAGVLEVAAVVGLLVVFVVGVLDDLAGGGPRGLGGHLRSLA
                     SGRPTTGILKLVTGIAAGVAAAVAAGGGPLRIVAGAVVVATAVNVLNALDVVPGRSLK
                     WGVIALAACQPAAWGTRVGSLVAAALGAAVASLPFDLGERAMLGDGGSNPLGFLVGVA
                     LLVVLPTPGLLVAAVALLALQVAAETVTISRLIEVAPPLRWLDGLGQRRRP"
     gene            1768..>2688
                     /locus_tag="VF058_08520"
     CDS             1768..>2688
                     /locus_tag="VF058_08520"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX6208384.1"
                     /translation="MAEHTKYIFVTCGVSSSLGKGITAASLGRLFRSRGLRVSLQKLD
                     PYVNVDPGTMNPFEHGEVFVLEDGAETDLDLGHYERFVDENLYRGSNVTTGAIYSSVI
                     AKERRGEFLGKTVQVIPHITDEIKARIRGLARSTDADVIIVEVGGTVGDIESLPFLEA
                     IRQLRNEVGRDNCAFVHVSLLPHIGPTGELKTKPTQHSVRELRSIGIQPDAIVCRSDR
                     PIGHGLKEKISLLCDVEIGGVVSAVDADSLYEVPLTLHEEGLDTFLARHLRIDAEPDL
                     TEWRALVDRIRSAEGEVTIAVVGKYVNLPDA"
BASE COUNT          315 a          922 c         1041 g          410 t
ORIGIN      
        1 gagcgtggcg gccgccgaga gcgcgacggt gctggtgccg ttcgttcccc tgctccgcga
       61 gggtcccgtg tcggatcggc tcgtcaccca ggacaacgtg gatcaggtcc cgggcgaggt
      121 ttcgctcgtg ttggccctcg aggacctggt cgagctgggg gaggctggcc actacggggt
      181 gaagccgggc gcgaacggcc tggtgccctc gctcggctga tgcccgcgct ggcgttcgtg
      241 gcggcccgcg acgaggagag ccgtgtgggc gagaccgtcc gggcgatccg cgacctcccc
      301 gtggacgaga tcgtggtggt ggacgacggg tcgcgggacg ggacggcggc cgaggccctc
      361 gccgcgggcg cccgggtgct ggtcgccccg cgccccatgg ggaaggggga ggcgctggag
      421 gccgcgctcg accgggcgcc cgcggccgac gtctacctgt tcctggacgg ggacctgggg
      481 gcgacggcct cggccgccgt cccgctgctc gagctcgttc gggatgggaa ggccgacctg
      541 gcgatcgccg agctccctcg tcagccgggc cacggcggct tccgagttgt gaagcggctc
      601 tcggcccggc tgatccggag cctgtccggg ttccgggccc gcgagccgat gtccggccag
      661 cgggccatga cccgggaggt cctggaggct gtgcggccgc tggctccggg cttcggcgtg
      721 gagacggcca tgacgatcga cgcggttcgg gcggggttcc gggtgatgga ggtcgaggtt
      781 cccatgtccc accgggtcac cggtcgcgac ctcgccggct tcgcgcaccg ggcgcgtcag
      841 ggtagggacg tcctggccgc ggcctggccc cgggcgatcg ggcggcgatg acctgggtct
      901 cgctggcggc gggggccctg gttggggcgg cgctggcctg gttctcgcag ctgaggcccc
      961 cgcgggggct cgtccgagag aaccatgcgg ggctgccggt gcccgcggtg ctggggctcg
     1021 ccctcgtggg gggcgcggtg gtggggcacg ccgtggggcg accggaggcg gaggccggcg
     1081 tcttggaggt ggccgcggtc gtcggcctgc tggtcgtctt cgtggtcggg gtgctggacg
     1141 acctggccgg cggggggcct cggggcctgg ggggccacct gcggagcctg gcgagcggcc
     1201 ggcccacgac ggggatcctg aagctcgtga ccgggatcgc ggcgggcgtg gccgcggcgg
     1261 tcgcggcggg cggcggaccg ctccggatcg tggccggggc cgtggtggtc gccaccgccg
     1321 tcaatgtcct caatgcgctg gacgtggtcc cggggcgatc gctgaagtgg ggggtgatcg
     1381 ccctggccgc gtgccagccc gcggcctggg gaacgagggt cggctcgctc gtcgcggccg
     1441 ccttgggagc cgccgtcgcc tcgctcccgt tcgacctcgg tgagcgggcg atgctcggcg
     1501 acggcggctc caaccccctc ggcttcctcg tgggggtggc cctgctcgtg gtgctgccga
     1561 ccccggggct gctcgtcgcc gcggtcgcgc tgctggcgct ccaggtcgcc gccgagaccg
     1621 tcacgatcag ccgcctgatc gaggtagcac cgcccttgcg gtggctcgac gggctggggc
     1681 agcggcgccg tccctagtgg ggtccgggat ccgggtcacc gaaaggccac acgcctcctg
     1741 ggccttctgc taccatggcc tcccgaggtg gctgagcaca cgaagtacat cttcgttacc
     1801 tgcggggtgt cgtcgagcct cgggaagggc atcaccgccg cgtctctggg tcgcctcttc
     1861 cgctcccgcg ggctccgcgt ctcgctccag aagctcgacc cctacgtcaa cgtcgacccc
     1921 gggacgatga atcccttcga gcacggggag gtcttcgtcc tcgaggacgg cgcggagacc
     1981 gatctcgacc tcgggcacta cgagcgcttc gtggacgaga acctctaccg aggctccaac
     2041 gtcaccaccg gggccatcta ctcctcggtg atcgccaagg agcggcgcgg cgagttcctg
     2101 ggcaagaccg tccaggtcat cccgcacatc accgacgaga tcaaggcccg gatccggggg
     2161 ctcgcccgat ccaccgacgc cgacgtcatc atcgtcgagg tcggggggac ggtgggcgac
     2221 atcgagtccc tcccgttcct ggaggcgatc cgccagctcc gaaacgaggt ggggcgcgac
     2281 aactgcgcgt tcgtgcacgt ctcgctgctg ccccacatcg gccccaccgg cgagctgaag
     2341 acgaagccca cccagcactc ggtgcgcgag ctgcgatcga tcggtatcca gcccgatgcg
     2401 atcgtgtgcc gctcggaccg tccgatcggc cacggcctca aggagaagat ctcgctcctc
     2461 tgcgacgtcg agatcggtgg ggtcgtctcg gcggtggacg cagactccct gtacgaggtc
     2521 ccgctgacgc tgcacgagga ggggctggac acgttcctcg cccgccacct gcgcatcgac
     2581 gccgagcccg acctcaccga gtggcgggcg ctcgtggacc ggatccgctc ggcggagggc
     2641 gaggtgacga tcgccgtcgt cgggaagtac gtgaacctgc ccgacgcc
//
LOCUS       DASUTW010000249         5340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1634357, whole genome shotgun sequence.
ACCESSION   DASUTW010000249 DASUTW010000000
VERSION     DASUTW010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1634357"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VF058_08525"
     CDS             <1..191
                     /locus_tag="VF058_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208385.1"
                     /translation="IPNIVNVAVVAAVGLLLWLSIRGRLESIERRMDRLDDSVNALRS
                     DLMQVALAVGAKPRSGSG"
     gene            complement(341..1024)
                     /locus_tag="VF058_08530"
     CDS             complement(341..1024)
                     /locus_tag="VF058_08530"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX6208386.1"
                     /translation="MRAVRSGSAPESAVERAYRMTREAILTGEYPPGFPLRVGEIAER
                     SGVSSIPVREALRRLEAERFVESEANKGARVAELSFPDLADAYRLRILLEVEAVRLAF
                     GDLAEEDLLQARRLLEDMTRLWSEGRIEEGHEAHRRLHFLIYEKAGSTWLLHVITSIW
                     DHTERYRRLALRWGAEPEELGPRHEALLETLHGSDLDAVIAAVRQHFEDPMRAIESRL
                     RATEGAEDL"
     gene            1108..1737
                     /locus_tag="VF058_08535"
     CDS             1108..1737
                     /locus_tag="VF058_08535"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HEX6208387.1"
                     /translation="MATTGTELPRPAIRDEIVPARGVTAFEILAGQTVRIEDVEGQQA
                     VDLICYNLHDLAEKFWAAHTAKLNGTIYVTTGHVLYSDQANPMMTFVEDTVGVNDLIC
                     GSCSHVLDVLRYGEEKAAPKGCMDLFEESIRPWGLERKDVPMCLNIFLDYPVEEGGHV
                     AIDKDAPSKPGDHVDLRAEMDLLVSITACPQENNPCNGFNPTPIRVAVY"
     gene            1741..2202
                     /locus_tag="VF058_08540"
     CDS             1741..2202
                     /locus_tag="VF058_08540"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6208388.1"
                     /translation="MTRKTVIDANYGRGIDLLRGAEGTDQLVHSKGIRLDLPGFTWIM
                     LSGVVALDDDLQLVGEGDIRVQTRFVLEQIQADLRRQGASMDDICRVRVYVSGLTPER
                     FRAIHEERARFFRSEHYPASTLVEVAGLIKPELLIEIDVDAMILGDDDGHE"
     gene            2236..3258
                     /locus_tag="VF058_08545"
     CDS             2236..3258
                     /locus_tag="VF058_08545"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6208389.1"
                     /translation="MTRLRWLRVAALVGALALVASACGDDGGGEGDGDLPTVVFQGFP
                     ADPAALPLLVMQEEGLDRDNGFIGEYLAVDPDAATNTFLIGESDIAMEQDGVNMTIVQ
                     QEGHEAVLFAPGLNMVTGIVVAEDSPYQDPTDLVGERVGHFGIDSGTTSTIALMIREI
                     YGLDILSEYDFRETGPEALPELLKSGEVEAILDFEPLALRAVLETPGRYLFEPTKAWA
                     EHTGGWSPWLTNLAAREDWLAENEDIAIGIRDAWMEGIRILEDSDYELLREEPYNSFM
                     ELKSDEELDAFIEYCADLPCFGSSWTEEDITELNNYLGLFAEHDILIEETASEPVAVI
                     LEDYYG"
     gene            3286..4098
                     /locus_tag="VF058_08550"
     CDS             3286..4098
                     /locus_tag="VF058_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6208390.1"
                     /translation="MTERPNSPTGALRTRRRVSWLDLALGAAGLGSLLLLWWAGSRTT
                     TFVPQISEVVAGLPEFLTDEETWPDVLATVRRVFGALAIAMVAGFLAAYVMHRGGLWG
                     RVMERYVSIMLGIPSTIAALLALFIFKRSEIGVYFVVSIITFPFVALTLLQGLRAADK
                     RLDEMGEAYRLPTSAHARHIAAPHLIPYTFAALRNEYAHAWKVVVLAELFAVNTGMGA
                     RFARAFDRFLLVDAMHWLIVFMVILLGTEFLLLLPTERRVLRWRGSGKEAAT"
     gene            4095..4901
                     /locus_tag="VF058_08555"
     CDS             4095..4901
                     /locus_tag="VF058_08555"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6208391.1"
                     /translation="MSTERVSAPGGGHGYIRVENLRVSYDGVTPALTDLHMEVEKGEF
                     VAVLGPSGCGKSTLLHVMSGVMRPQAGRIEIGGRAIDGSAGADAPPIGYVFQDHRLLP
                     WRTVAQNIAIVLRAAGVPKEEIRRRTDRYLEMLHIDAFRDMWPMRLSGGQRQRASIAR
                     ALALQPAVVLMDEPFSTLDEVTARLMRQQLAELWQASEQTIVFVTHSIREALFLADRI
                     YILTKGPARVFDVVDVPIDRPRRYEDPKLTELEGEIVDRVMDVWGLGGAV"
     gene            4901..>5340
                     /locus_tag="VF058_08560"
     CDS             4901..>5340
                     /locus_tag="VF058_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208392.1"
                     /translation="MATSDQATARAGGMLRSRDRVERAQAFVVASLLALAIWQSATLI
                     SDGWVPSLARIFTAFVEDATTLDVWGQIWITFRRILITFSAATVIGIVLGVAMGLSRR
                     VTAFFRPIVVMGLAVPDPVYIIVAILVLGTDESSGLIALVIALVP"
BASE COUNT          863 a         1799 c         1821 g          857 t
ORIGIN      
        1 tgatccctaa catcgtcaac gtggcggtcg tcgccgcggt gggattgctc ctctggttgt
       61 cgatcagggg caggctggaa tcgatcgaac gccggatgga ccgactcgac gactccgtga
      121 acgctctgcg ctccgacttg atgcaggtcg cgctggccgt cggcgcgaag ccgcgctccg
      181 ggtccggatg actcaacacc ccaggacatc cttgagctgt tgggcctgta acgccgtccc
      241 cacggtgacc ggaggctact gatgaccagg actgagcgaa gggtcgacca cgagaacccc
      301 aagcgcatcc cccgcccggt ccccggtgga accgagagca tcagagatcc tcggcgccct
      361 ccgtggcgcg gaggcggctc tcgatcgcgc gcatcggatc ctcgaagtgc tggcgaaccg
      421 cggcgatcac cgcgtccaga tcgctgccgt ggagcgtctc gagcagcgcc tcgtgcctcg
      481 gcccgagctc ctcgggctcc gccccccacc gcagcgccag gcgccggtac cgctcggtgt
      541 ggtcccagat cgaggtgatc acgtgaagga gccacgtcga tcccgccttc tcatagatca
      601 ggaagtgcag ccgccggtgc gcctcgtgcc cctcctcgat ccggccctcc gaccacagcc
      661 gggtcatatc ctcgaggagc cggcgggcct ggagcaggtc ctcctccgcc agatccccga
      721 acgccaaccg gacggcctcc acctcgagga ggatccgcaa ccgatacgcg tcagctaggt
      781 cagggaacga cagctcggcc acccgtgcac ccttgttggc ctccgactcg acgaaccgct
      841 cggcctccaa ccgccgcagg gcctctcgga cggggatcga actgacgccg ctccgctcgg
      901 cgatctctcc cacccttaat gggaagcccg gcgggtactc gccggtgaga atggcctctc
      961 gggtcatacg gtacgctcgc tccacagccg actcgggggc gctacccgac cgtaccgccc
     1021 tcatccaatc cctccccgat ttgaccaggc cccggaacgg ttctatatct tatcgtatat
     1081 ctcatactca gcagaggagg cccctgaatg gcgaccaccg ggaccgagct cccccgcccc
     1141 gcgatccgcg acgagatcgt ccccgccagg ggcgtcaccg cgttcgagat actggcgggc
     1201 cagacggtca ggatcgagga cgtcgagggg cagcaggccg tcgacctcat ctgctacaac
     1261 cttcacgatc tcgcggagaa gttctgggcc gcgcacaccg ccaagctgaa cggcactatc
     1321 tacgtcacca ccggtcacgt gctctattcc gaccaggcca acccgatgat gaccttcgtc
     1381 gaggacaccg tcggggtgaa cgacctcatc tgcggatcat gctcccacgt cctagacgtg
     1441 ctccgctacg gggaagagaa ggcagctccc aaggggtgca tggacctctt cgaggagtcg
     1501 atccggcctt gggggctcga gcggaaggac gtcccgatgt gcctgaacat cttcctcgac
     1561 tacccggtgg aggagggcgg gcacgtcgcc atcgacaagg acgccccctc caaacccggg
     1621 gaccacgtgg acctgcgggc cgagatggac ctcctcgtct cgatcacggc ctgcccccag
     1681 gagaacaacc cgtgcaacgg cttcaacccg accccgattc gggtggcggt gtactgaagg
     1741 atgaccagga agaccgtgat cgatgccaac tacggcaggg ggatcgacct gctgcgtggc
     1801 gccgagggca cggaccagct cgtccactcc aaggggatcc gcctcgacct ccccgggttc
     1861 acctggatca tgctctccgg cgtcgtggcc ctcgacgacg acctccagct ggtcggcgag
     1921 ggcgacatcc gggtgcagac ccggttcgtg ctggagcaga ttcaggcgga cctccggcgc
     1981 cagggcgcct cgatggacga catctgtcgg gtccgcgtgt acgtgtcggg gctcaccccc
     2041 gagcgcttca gggcgatcca cgaggagcga gcgaggttct tccgctcaga acactacccg
     2101 gccagcacgc tggtggaagt ggccggcctg atcaagccgg agctcctcat cgagatcgac
     2161 gtcgacgcca tgatcctcgg cgacgacgat gggcatgagt agcgattcgg ccgcgaggcc
     2221 gggagggagg ggacgatgac aagactcagg tggctcaggg tggcggccct ggtgggggca
     2281 ctcgcgctcg tcgcctccgc ctgcggcgac gacggaggag gcgaggggga cggcgacctc
     2341 ccgaccgtcg tcttccaggg attcccggcg gacccggccg cgctccccct gctcgtgatg
     2401 caggaggaag ggctcgatcg ggacaacggg ttcatcggcg agtacctcgc ggtcgacccc
     2461 gacgccgcca cgaacacctt cctgatcggc gagagcgaca tcgccatgga gcaggacggg
     2521 gtcaacatga cgatcgtcca gcaggaaggc cacgaggcgg tgctgttcgc gcccgggctc
     2581 aacatggtga ccgggatcgt ggtggccgag gattccccct accaggaccc gacagacctg
     2641 gtcggtgagc gggtgggcca tttcgggatc gactcgggaa ccacctcgac gatcgcgctg
     2701 atgatccggg agatctacgg cctcgacatc ctcagcgagt acgactttcg ggagacgggg
     2761 cctgaggccc tccccgaact gctgaagagc ggcgaggtgg aggcgatcct ggacttcgaa
     2821 ccgctggcgc tgcgggcggt cctggagacg cccggtcggt acctgttcga gccgactaag
     2881 gcgtgggctg agcacaccgg cgggtggtcg ccgtggctga cgaacctcgc ggcccgtgag
     2941 gactggctcg ccgagaacga ggacatcgct atcggcatcc gcgacgcctg gatggagggg
     3001 atccggatcc tcgaagactc ggactacgag ctgctccggg aggagccgta caactccttc
     3061 atggagctga agagcgatga ggagctggac gcgttcatcg agtactgcgc cgacctgccg
     3121 tgcttcggct cgagctggac cgaagaggac atcaccgagc tgaacaacta cctggggctg
     3181 ttcgcagagc acgacatcct catcgaggag acggcctcgg agcccgtcgc cgtgatcctg
     3241 gaggactact acggctaggc tccgggcacg gagcggggtg aggcggtgac ggagcggccc
     3301 aacagcccga cgggcgccct gaggacgagg agacgggtct cgtggctcga cctcgccctc
     3361 ggggctgccg ggctcgggtc gctgctgctc ctctggtggg cggggagccg cactacgacg
     3421 ttcgtcccgc agatcagcga ggtcgtcgcc ggcctcccag agttcctcac cgacgaggag
     3481 acctggccgg acgtgctggc caccgtccgg cgcgtgttcg gcgcgctggc gatcgccatg
     3541 gtggccggct tcctcgccgc ctacgtgatg caccgcggcg gcctgtgggg gcgggtgatg
     3601 gaacggtacg tgagcatcat gctcgggatc cccagcacga tcgcggcgct cctcgcgctg
     3661 ttcatcttca agcggtcgga gatcggcgtg tacttcgtcg tgtcgatcat cacgttcccg
     3721 ttcgtggcgc tgaccctgct gcaggggctc cgggccgcgg acaagcggct ggacgagatg
     3781 ggggaggcct accggctgcc cacctcggcc cacgcgcgcc acatcgccgc cccgcacctg
     3841 atcccctaca cattcgcggc cctccgcaac gagtacgcgc acgcctggaa ggtcgtggtg
     3901 cttgcggagc tgttcgcggt caacaccggc atgggggcgc ggttcgcccg ggcgttcgac
     3961 cggttcctgc tggtcgacgc gatgcactgg ctgatcgtgt tcatggtcat cctcctgggg
     4021 acggagttcc tcctgctcct cccgacggag cggcgggtct tgcgatggcg tggctcgggg
     4081 aaggaggccg ccacgtgagc acggaacggg tgtcggcgcc cggaggtgga cacggctaca
     4141 tccgcgtgga gaacctcagg gtctcctacg atggcgtgac gccggccctg accgacctcc
     4201 acatggaggt cgagaagggc gagttcgtgg ctgtcctcgg tccgagcggt tgcgggaagt
     4261 ccacgctgct gcatgtgatg tccggggtga tgcggcccca ggcggggcgg atcgagatcg
     4321 gcggccgggc catcgacggg tcggccggtg cggacgctcc cccgatcggc tacgtcttcc
     4381 aggaccaccg cctcctcccg tggcgaaccg tggcccagaa catcgccatc gtcctgcgcg
     4441 cggccggggt ccccaaggag gagatccgcc ggaggaccga ccgctacctc gagatgctcc
     4501 acatcgacgc cttccgggac atgtggccga tgcggctgtc cggcggacag cggcaacggg
     4561 cctcgatcgc tagagcgctt gcgctccagc cggcggtggt gctgatggac gagcccttca
     4621 gcaccctgga cgaggtgacg gcacgactca tgcgccagca gctcgccgag ctgtggcagg
     4681 cgagcgagca gacgatcgtg ttcgtgaccc actcgatccg ggaggcgctg ttcctcgccg
     4741 accggatcta catcctgacg aagggtccgg ccagggtctt cgacgtggtg gacgtcccga
     4801 tcgaccggcc gcgccggtac gaggacccga agctcacgga actggagggt gagatcgtgg
     4861 accgggtgat ggacgtgtgg ggcctcggcg gagcggtgta gtggccacct cggaccaagc
     4921 gacggcgcgc gccgggggga tgctccggtc gcgggaccgg gtcgagcgag cgcaggcgtt
     4981 cgtcgtggcg tcgctcctcg ccctcgcgat ctggcagtcc gccaccctga tcagcgacgg
     5041 ctgggttccg tcgctcgcgc gcatcttcac ggcgttcgtc gaggacgcca cgacgctcga
     5101 tgtgtggggt cagatctgga tcaccttccg gcggatcctc atcacgttct cggccgccac
     5161 ggtcatcggg atcgtcctcg gggtggcgat gggcctgagc cggcgggtca cggcgttctt
     5221 ccggccgatc gtcgtgatgg ggctggccgt ccccgacccc gtctacatca tcgtggcgat
     5281 cctggtgctc gggaccgacg agtcgtcggg actcatcgcc ctggtcatcg cgctcgtccc
//
LOCUS       DASUTW010000250         2881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_599610, whole genome shotgun sequence.
ACCESSION   DASUTW010000250 DASUTW010000000
VERSION     DASUTW010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599610"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VF058_08565"
     CDS             complement(<1..516)
                     /locus_tag="VF058_08565"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX6208393.1"
                     /translation="MHVVATDAHRDHAPRTEFVLGEVVPAYEVPDRVDRILEAISEAH
                     PLGEPTEHGRRPLERIHAPAYLDHLESAWRDWVAEGGAGPLVADTFALPGVAAGPRPR
                     GAEARVGAYTFDASTPILEGTWRAAAAAADCALTGADLLLGGERAAYALCRPPGHHAG
                     RAFAGGYCYLNS"
     gene            750..1382
                     /locus_tag="VF058_08570"
     CDS             750..1382
                     /locus_tag="VF058_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208394.1"
                     /translation="MNEQSLRQSNGILAAAAERAAAGELIPATPAELARAVGVDDRLA
                     VARAVRALLARGRLEQEGDRYRLLDTRPLEPGEPATVRRPSRRRAKSREAETQDEGVP
                     TYEQVGRAIVERLIELSAESAELRASLERARSESEAARREALEATRAASRERQRATDM
                     EQESRMLRQRLEMTESNLRRIIEAAQSRPAAPLEDSDAKAILDVLSKKDA"
     gene            1528..2781
                     /locus_tag="VF058_08575"
     CDS             1528..2781
                     /locus_tag="VF058_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208395.1"
                     /translation="MSLIAQIPSDFLGLSASQWFWLAIVGWSLSVVLGGLLAARAVQG
                     APGWGEVGPERIALDTPAILRAVAPDEDAAGGVLRWLLGEVDADAVAHLELSPRGEML
                     RIEPRGLEEASVARLAELARSALLGSGEPAEGAGVARWLGAGGTNALVAAGTTVQGHE
                     EPLRFSRYLLEWVRSAERGRPRGDLEDRLREVPGVTWVEVAGRTARLLAEDEATADVT
                     LAEVARRLEGTGIAPVLIDAARPDVHVAEAVGRPEASMVAIPEAEPSEAQRVPTEPIG
                     TKEPLMASAIGGQEPRIRLLDVALRENGATTADVRVSWNKQEIRGRGHGRATQAGRSF
                     AAAQAVADALRPLLDTDVVIEGLYVATTKEGLDVLISEVRMEGERYVGAVLERRDRPD
                     WTGARAVLDAVNRRLVQVAGRSGRI"
BASE COUNT          382 a         1027 c         1109 g          363 t
ORIGIN      
        1 gctgttgagg tagcagtagc cgccggcgaa ggcccggccg gcgtgatggc cgggaggccg
       61 gcagagcgcg taggcggccc gctcgccccc cagcaggagg tcggcccccg tgagggcgca
      121 gtccgccgcc gccgcggccg ccctccacgt cccctcgagg atcggcgtgg aggcgtcgaa
      181 ggtgtaggcc ccgacccggg cctcggcgcc ccgcggtcgc ggcccagcgg cgacgccggg
      241 gagggcgaac gtgtcggcca cgagcggtcc ggcgcccccc tcggccaccc agtcccgcca
      301 cgcggactcc aggtgatcga ggtaggccgg ggcgtggatc cgctccagcg ggcgtcgccc
      361 gtgctcggtg ggctcgccca gcggatgggc ctccgagatg gcctcgagga tccggtccac
      421 cctgtcgggg acctcgtagg cgggaaccac ctcgcccagc acgaactcgg tccgcggggc
      481 gtgatcgcgg tgagcgtcgg tggcgacgac gtgcatcgga gcagtctgcc cggcgcggcg
      541 gccgggggca agggaaagac ggaaccgcga gagtccgaag actcccgcgg ttccaaggcg
      601 atttgtacac cgagctctgg ccccgcgcaa ggcccagccc tgcttttgtc actctttctc
      661 ggttcccgct gccggggcgg tcgcgttcgg ggggttcgtc ccggtaccac ctccctccca
      721 tgcagcccga cggtgtacaa atacagtcca tgaacgagca gagcctgcgg cagagcaacg
      781 gcatcctggc ggcggccgcc gagcgggcgg cggcgggaga gctgatcccc gccacccccg
      841 ccgagctggc gcgggcggtc ggggtggacg atcgcctggc ggtggccagg gccgtccgcg
      901 ccctgctggc ccggggccgg ttggagcagg agggcgaccg gtaccgcctc ctcgacacga
      961 ggccgctcga gcccggcgag ccggcaacgg ttcgtcgccc ctcgaggaga cgcgccaaga
     1021 gccgggaggc cgagacccag gacgaggggg tccccaccta tgagcaagtg gggcgggcga
     1081 tcgtggagcg tctgatcgag ctctcggccg agtccgcgga gctcagggcc tcgctcgagc
     1141 gggcccgttc cgagagcgag gccgcccgcc gagaggcgct cgaggcgacc cgcgccgcct
     1201 cacgggagcg ccagcgggcc acggacatgg agcaggagtc ccggatgctg cgccagcgcc
     1261 tcgagatgac cgagtccaac ctccggcgca tcatcgaggc cgcccagagc aggcccgcgg
     1321 ccccgctcga ggactccgac gccaaggcga tcctggacgt tctctccaag aaggacgcct
     1381 gaggattccc ccagacgacc gacggggcta ccacccccgg ggatgacgcc gttgggcgat
     1441 tgcgcacatg cgctgccctc tgctttcctg acctggggcc gctcgtcgtt ggaaggaggt
     1501 tgtgtgcccg ggccgatcct gggttccatg tccctcatcg ctcagatccc gtccgacttc
     1561 ctcggcctct cggcctccca gtggttctgg ctcgccatcg tgggctggtc cctgagcgtg
     1621 gtcctcggcg gcctgctcgc cgcccgcgcc gtccagggcg ctcccgggtg gggagaggtg
     1681 ggcccggagc gcatcgcgct cgacaccccc gcgatcctcc gggcggtggc gcccgacgag
     1741 gacgcggcgg ggggtgtgct tcggtggctg ctgggggagg tcgatgcaga cgccgtggct
     1801 cacctcgagc tctccccgcg tggggagatg ctgcggatcg agccccgcgg gctggaggag
     1861 gcgtcggtcg cccggctggc cgagctcgct cgatcggccc tgctggggtc cggggagccc
     1921 gccgagggcg ccggcgtcgc gcgctggctc ggtgccggcg ggacgaacgc gctcgtggcc
     1981 gccggcacga cggtccaggg gcacgaggag cccctccggt tctcgcgcta cctgctcgaa
     2041 tgggtgcgat cggcggagcg tgggcgtccg cggggggacc tcgaggaccg ccttcgagag
     2101 gtcccgggtg tgacgtgggt ggaggtcgcg ggccggacgg cccggctcct cgcggaggac
     2161 gaagcgaccg ccgacgtcac gctggccgag gtggcccggc ggctcgaggg caccggcatc
     2221 gccccggtat tgatcgacgc ggcccggccg gacgtccacg tagccgaggc ggtgggtagg
     2281 ccggaggcct ccatggtggc catcccggag gccgagccgt ccgaggccca gcgcgtcccc
     2341 accgagccga tcggcacgaa ggagcccctc atggcgtcgg cgatcggcgg acaggagccg
     2401 aggatccggc tgctcgacgt cgccctccga gagaacggcg cgaccacggc ggacgttcga
     2461 gtgagctgga acaagcagga gatccgggga cggggccacg ggcgcgccac gcaagccggc
     2521 cggtccttcg ccgcggcgca ggcggtggcc gacgcgcttc gtccgctcct ggacaccgac
     2581 gtcgtgatcg agggcctcta cgtcgccacg acgaaggagg gtctcgacgt cctgatctcc
     2641 gaggtcagga tggaggggga gcggtacgtg ggagcagtcc tggagcgccg ggaccggcca
     2701 gactggacgg gggcccgcgc cgtcctcgac gcggtcaacc gccggctggt gcaggtcgcc
     2761 ggccgcagcg gtcggatctg agcgctcacc gccgccggat cagcaggagc gtccccgtca
     2821 gcaccgcggc caccgccagg gcggcgaccg cgactcccag gggcaccgcc ggggccccct
     2881 c
//
LOCUS       DASUTW010000251         2795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_599669, whole genome shotgun sequence.
ACCESSION   DASUTW010000251 DASUTW010000000
VERSION     DASUTW010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599669"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..1383
                     /locus_tag="VF058_08580"
     CDS             268..1383
                     /locus_tag="VF058_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX6208396.1"
                     /translation="MRLSYSSISTYERCPAQFRFQYEERLPTAPGPALSFGASLHEAL
                     HRFHDRPVPVAPPVEELLQILDEVWTSEGFRTPEEERPYLEHGRQVLRQYHLDNAADY
                     RIPAALEHHFSIDVDGVTVSGVIDRMDRLPGGGYEIIDYKTNRRLPPKRVVEDDLQLS
                     IYALAAREVWGIEPERLTLYYLLPGQRMTTTRSPAQLDDLRRRVAAVAERIEAGRFEP
                     RENRLCDWCDFQPRCPVFRHRYEEDGPPKIKEIVDEWVRLKREDRERWRRLEELGATI
                     RAYAEEHGLKRLYGSEGAVALVRRVEQAIDPARVRELLEPLGRYEEVLSVDPRALGDL
                     VERRQLPPDVEDALLASREAVRVTTALHLRELDRSRR"
     gene            1407..1823
                     /locus_tag="VF058_08585"
     CDS             1407..1823
                     /locus_tag="VF058_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208397.1"
                     /translation="MPPRKKSLAARVRATAEPFLGEKERVLRAAEAMKGTRTTLAALL
                     GPIGYFLVLRPRHVALTNRRIIVATPPARGGGAPKLDLALDRTATEVVEHRPGRLWTR
                     LILQDEGGRRVALNFARLWHEEAEFLRRRLTRRERA"
     gene            complement(1801..2694)
                     /locus_tag="VF058_08590"
     CDS             complement(1801..2694)
                     /locus_tag="VF058_08590"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX6208398.1"
                     /translation="MTRVTVRGAPGRLDAVLALAAGISRADAQRAIEAGGVLVDGEPR
                     PKSHRLRGGEQIEAELPEAGRLEPEPGGVPVRYEDEHLLIVAKPPGLVTHPTASRRTG
                     TLVNRLLGMGVSLSAGSDPDRPGIVHRLDAGTSGLMLVAKSDAAHEGLVRLLARRAVE
                     REYLALVRGVAEHDDFAVEAPLERRGARVRVRRGGGAEAETRFSVLERLEGATLLSAR
                     PRTGRTHQIRVHLASIGHPILGDRAYGGGGDDARALGLGRPFLHAHRLALTHPITGER
                     IEVEESLPEDLEAALRRARGE"
     gene            complement(2691..>2795)
                     /locus_tag="VF058_08595"
     CDS             complement(2691..>2795)
                     /locus_tag="VF058_08595"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX6208399.1"
                     /translation="FIDFHVWPVFNVADSAIFLGAATLLLLGLRRSEP"
BASE COUNT          379 a         1069 c          986 g          361 t
ORIGIN      
        1 gaggccgatc gccggcatcc ccatctcggc ggcccggtcg agcatcgagg gcgcgctgct
       61 gcccggcgtg atgcgcgcga acccgtccag gagcgagaac tccgtgtgcg tgtggagatg
      121 gacgaagctg ctgctcactg cccctccggg ggaagggtcg gccacggcct cccgcgctgt
      181 ggataacctg tggattgatt acacccttgt aattcctgcg ctcccaaatc tatactccgg
      241 cccgaggccg gtcaagggga gacggcgatg cgcctgtcct actcgagcat ctccacgtac
      301 gaacggtgcc ccgcgcagtt ccgcttccag tacgaggagc ggctgcccac ggcgccgggg
      361 ccggcgctgt ccttcggggc ctcccttcac gaggcgctcc accgcttcca cgaccggccg
      421 gtgccggtgg cgccccccgt ggaggagctc ctgcagatcc tggacgaggt gtggacctct
      481 gaggggttcc gaacccccga ggaggagcga ccctacctgg agcacgggcg gcaggtgctt
      541 cgccagtacc acctcgacaa cgcggccgac taccggatcc cggcggccct cgagcaccac
      601 ttctcgatcg acgtggacgg cgtcacggtc tcgggcgtga tcgaccgcat ggaccgcctc
      661 cccggcggcg gctacgagat catcgactac aagacgaacc gccggctccc gccgaagcgc
      721 gtcgtcgagg acgacctcca gctctcgatc tacgcgctgg ccgcgcgcga ggtctggggc
      781 atcgagcccg agcggctgac cctctactac ctcctccccg gccagcggat gaccaccacc
      841 cgctccccgg cccagctcga cgacctccgc cggcgggtcg ccgcggtggc cgagcggatc
      901 gaggcggggc gcttcgagcc ccgggagaac cggctttgcg actggtgcga cttccagccg
      961 cggtgccccg tgttccgcca ccgctacgag gaggacggcc cgccgaagat caaggagatc
     1021 gtggacgagt gggtgcggct caagcgcgag gaccgcgagc ggtggcgccg cctggaggag
     1081 ctgggggcca cgatccgggc ctacgccgag gagcacggcc tgaagcggct gtacgggagc
     1141 gagggcgcgg tggcgctcgt ccggcgggtg gagcaggcga tcgatcccgc ccgcgtccgc
     1201 gagctcctcg agccgctggg ccgctacgag gaggtcttgt ccgtggatcc ccgcgcgctg
     1261 ggggacctcg tcgagcggcg ccagctgccg cccgacgtcg aggacgccct gctcgcttcc
     1321 cgggaggccg tccgggtgac caccgctctc cacctccgcg agctcgaccg cagccggcgg
     1381 tgacggcgct ccgctaggct gagccggtgc cacccaggaa gaagagcctc gccgcgcggg
     1441 tccgcgccac cgcagagccg ttcctcgggg agaaggaacg ggtgctgcga gccgccgagg
     1501 cgatgaaggg gacccggacc acgctggccg ccctgctggg gccgatcggg tatttcctcg
     1561 tcctgcgccc ccgccacgtg gcgctcacga accggcggat catcgtcgcg acccccccgg
     1621 cccggggcgg gggggcgccc aagctcgacc tggccctgga ccgcacagcc accgaggtcg
     1681 tcgagcaccg tcccgggagg ctctggaccc gcctcatcct ccaggacgag gggggccgcc
     1741 gcgtcgccct caacttcgcc cgcctctggc acgaggaggc cgagttcctc cgccggcgcc
     1801 tcactcgccg cgagcgcgcc tgagcgccgc ctccagatcc tcggggagcg actcctccac
     1861 ctcgatccgc tcaccggtga tcgggtgggt gagggccagg cggtgggcgt ggaggaacgg
     1921 cctgcccagg ccgagcgccc tcgcgtcgtc ccctcccccg ccgtaggccc ggtcccccag
     1981 gatcgggtgg ccgatcgagg cgaggtggac ccggatctgg tgggtccgtc cggtccgggg
     2041 ccgagccgac agcagcgtgg cgccctccag gcgctccagg accgagaacc tcgtctcggc
     2101 ctccgcgccg ccaccacgcc ggacgcgcac cctcgccccc cggcgctcca gcggggcctc
     2161 cacggcgaag tcgtcgtgct cggcgacgcc gcgcacgagg gcgaggtact cgcgctcgac
     2221 ggcacgccgg gcgaggagcc tgaccaggcc ctcgtgcgcg gcgtcgctct tggccacgag
     2281 catcagcccg gaggtccccg cgtccagccg gtgcacgatg cccgggcggt cggggtcgct
     2341 gcccgcggag aggctcaccc ccatccccag cagccggttg accagggtgc cggtccggcg
     2401 cgacgcggtc gggtgcgtga cgaggcccgg cggcttggcc acgatcagga ggtgctcgtc
     2461 ctcgtaccgg accggcaccc cgcccggctc cggctcgagc cgcccggcct cgggcagctc
     2521 ggcctcgatc tgctcgcccc cccggaggcg gtgcgacttc ggcctcggct ccccgtccac
     2581 cagaacgcct ccggcctcga tcgcccgctg ggcgtccgcc cgggagatcc cagccgccaa
     2641 ggccagcacc gcgtcgagcc ggcccggcgc cccccggacc gtgacccgtg tcacggctcc
     2701 gagcggcgca gccccaggag cagcagcgtg gccgctccca ggaagatcgc gctgtcggcc
     2761 acgttgaaca ccggccagac gtggaagtcg atgaa
//
LOCUS       DASUTW010000252         3652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_208049, whole genome shotgun sequence.
ACCESSION   DASUTW010000252 DASUTW010000000
VERSION     DASUTW010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208049"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2182)
                     /locus_tag="VF058_08600"
     CDS             complement(<1..2182)
                     /locus_tag="VF058_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX6208400.1"
                     /translation="MLQEILGPDEVDAHLLDHLMARTDGNPLFVEEILSTLDEASGGA
                     AIERLDVPPTIQEAIQRRVERLPGRARRVLQVAAVIGERFELEPIARVLGLGEDEALE
                     DLRALVREHLVLEEPSGQLVFRHALTRDSIYEPLLGEERRRLHRDVARALQDLHEDDL
                     EERAAEIATHFAAAEQAPEARRFAEMAAARASALGAPADARGHLRRALELTDDPHDRA
                     RTLLAIGNLSRSVGDLRTAAEELRLAAEMFEELGDRRLRAHALLDLALPTLMEGDRAG
                     ALRLLESVLGMLESEGESEELAKAYRALAGHYMLGANLSEAVRWGRRAVDLAERVGAD
                     EVLLDARIDVGTALASGDDREEGMSVLASVVEDAGSRGFLRLAARAAVNLSCVLVQNG
                     RYEEAMRAADEGRAISERGGEELGRRLCQSNLASARRLVGEWEEAERLLLDVLEGSKD
                     VASVKFAAMANLELVQLRADQGRWEDVQVVVDELEPYVLERPELQNLAPLRLAAARLH
                     AARGDVDRAWGELVALRDAWEKTDDTLLIAPSLGFAVELAAETGDRERGSEWLGLLEG
                     VAAASPSPEAAALLEEAAGVLAVSDSPAEAAERLAAAADAWRALGRPLDVARSLRWLA
                     EARLRSGAEARAVEPLEEAQRILVDLGAAHELALTRAALRRAGEKVPRGPRPSTREAP
                     GGLTRRELEVARLVGQGRSNAEIARALVISPKTSAAHVSNILTKL"
     gene            complement(2375..2860)
                     /locus_tag="VF058_08605"
                     /pseudo
     CDS             complement(2375..2860)
                     /locus_tag="VF058_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            2980..3396
                     /locus_tag="VF058_08610"
     CDS             2980..3396
                     /locus_tag="VF058_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6208401.1"
                     /translation="MAGNEHPNVRLAREGFAAFERGDMSWMDEHLADDIVWHVGGNSK
                     WSGAYEGKAAVLEFFGRQTGAMGGQPPSVEIHDVVGGDDHVVVLGNATATSPDGQSAE
                     WKYAQIFHIRDGKATEVWGMAENDADVDPFLDALPG"
     gene            complement(3445..>3652)
                     /locus_tag="VF058_08615"
     CDS             complement(3445..>3652)
                     /locus_tag="VF058_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208402.1"
                     /translation="EVALYAGGVRSATVVAERPSVLHRLTRDRLHEMEQRDPFAAAAL
                     HRLFARLLAERLTDTIRTVQALID"
BASE COUNT          495 a         1349 c         1344 g          464 t
ORIGIN      
        1 cgagcttcgt gaggatgttg ctcacgtggg cggcgctcgt cttcggggag atgacgagcg
       61 cccgcgcgat ctcggcgttc gagcggccct gcccgaccag gcgggccacc tcgagctccc
      121 ggcgggtgag ccctcccggc gcctcgcggg tggacggccg ggggccccgg ggcaccttct
      181 cgccggcgcg ccggagcgcg gcccgggtca gcgccagctc atgggccgcc cccaggtcga
      241 ccaggatgcg ctgggcctcc tccagcggct cgaccgcccg agcctcggcg cccgagcgga
      301 gccgggcctc ggccagccac cgcaggctcc gcgcgacgtc cagcggccgg cccagggcgc
      361 gccatgcgtc ggccgcggcg gcgagccgct cggccgcctc ggccgggctg tcgctcacgg
      421 cgagcacgcc ggcggcctcc tccagcagcg cggcggcctc cggcgagggg gacgcggccg
      481 cgacgccctc gagcaggccg agccactcgc tccccctctc ccggtccccc gtctcggccg
      541 ccagctcgac cgcgaagccc aggctcgggg cgatcaggag ggtgtcgtcg gtcttctccc
      601 atgcgtcccg gagtgccacg agctcgcccc acgcccgatc cacgtcgcct ctcgcggcgt
      661 gcagcctcgc cgcggccagg cggagcggcg cgaggttctg gagctcgggg cgctccagca
      721 cgtagggttc gagctcgtcc acgacgactt gcacgtcctc ccagcgcccc tggtcggctc
      781 ggagctggac gagctccagg ttggccatcg ccgcgaactt caccgaggcg acgtccttcg
      841 agccctccag cacgtcgagg aggagtcgct ccgcctcctc ccactcgccg accaggcggc
      901 gcgcgctggc gaggttcgac tgacacaggc ggcgacccag ctcctcgcct ccgcgctccg
      961 agatcgctcg cccctcgtcg gccgcgcgca tcgcctcctc gtaccgcccg ttctgcacga
     1021 gcacgcagct caggttgacc gcggcccggg ccgcaagccg taggaagccc cgggagccgg
     1081 cgtcctcgac gaccgaggcg aggaccgaca tcccctcctc acgatcgtcc cccgacgcga
     1141 gcgccgtccc gacgtcgatc cgggcatcga ggaggacctc gtcggcgccg acccgctcgg
     1201 cgaggtcgac cgccctgcgg ccccaccgga cggcctcgga gaggttggcg ccgagcatgt
     1261 agtggccggc cagggcccga taggccttgg ccagctcctc ggattcgccc tcggactcca
     1321 gcatccccag caccgactcg aggaggcgga gcgcgccggc gcggtcgccc tccatgagcg
     1381 tgggaagggc gaggtcgagg agagcgtgcg ccctcagccg tcgatccccg agctcctcga
     1441 acatctcggc agcgaggcgg agctcctcgg cagccgtccg caggtccccc acggagcgtg
     1501 agaggttgcc gatcgcgagc agggtcctgg cccggtcgtg ggggtcgtcc gtgagctcga
     1561 gggctcggcg caggtgaccc cgggcgtcgg ccggggcgcc gagcgcggag gccctcgcgg
     1621 cggccatctc cgcgaagcgg cgggcctccg gagcctgctc tgcggccgcg aagtgcgttg
     1681 cgatctcggc cgcgcgctcc tccaggtcgt cctcgtggag gtcctggagc gcccgggcca
     1741 cgtcgcgatg gagccttcgc cgctcctctc ccagcagcgg ctcgtagatg ctgtcccggg
     1801 tgagcgcgtg ccggaacacg agctggcccg agggctcctc caggaccagg tgctcccgca
     1861 ccagcgcgcg cagatcctcg agggcctcgt cctcgccgag gcccagcacc cgggcgatcg
     1921 gctcgagctc gaagcgctcg ccgatcaccg ccgcgacctg gagcacccgg cgggcccgcc
     1981 cgggcagacg ctccacccgt cgctggatcg cctcctggat cgtgggaggg acgtccaggc
     2041 gctcgatcgc ggcgccgccg gaggcctcgt cgagggtgct gaggatctcc tccacgaaca
     2101 gggggttgcc gtccgtgcgc gccatcaggt gatcgaggag gtgggcgtcc acctcgtccg
     2161 ggccgaggat ctcctggagc atcgtccgca cgtggtcgcg gtcgaggggg ccgaggacga
     2221 tctcccggca cagccgctgg cgccggagct ctgcgatcgt gtggactacg tccgggcggt
     2281 cggcgacctc atcgacccga tacgtcccca ccacgagcgc ggggatgtcg gggagggtcc
     2341 gggccaggat ggggaggagc tccacggtgg ccccgtccgc ccagtgcagg tcctcgagca
     2401 ccaacaggag cgggcggtcc acggcggccc cgaccctcgc ctcgcgttcg aggagcgtgc
     2461 agacggagcg agcgaccccg tagcgctcct cgctcgaggc gggagggtcc tgagtgggcg
     2521 cggggacgag ctcggggagg agacgcccca gccatcgctc ctcgacgccc gtgagcgcct
     2581 cggtcagtca gtcggacagg aggtcccgga gggcttcggc gatcggcgcg tacgagaggg
     2641 tccggagctg ctcgagacag gcgccgacgg cgatgcgtgc gcccagatcc tcggcgtgct
     2701 cacgcagccc acgcaggagg cgcgacttcc cgatccccgc ctcgccggag acgatcacga
     2761 gcccgccctc cgcccgcccg gcggaggcga gggcgtccgt gagcgccccc agctcctctc
     2821 gccggccgac caagacgggg cagacggcct tcgtcagcat gagagggaat catcgcatac
     2881 ggcgggcaac gcaggctcgg cggacgggag gcccgggtcg ctgttgacaa agcggggccc
     2941 caggcggtag gtctcgtccc actcgaggaa ggaggggtca tggcaggtaa cgagcacccc
     3001 aacgtcaggc tggctcgaga aggcttcgcg gccttcgagc gaggcgacat gtcctggatg
     3061 gacgagcacc tcgccgacga catcgtctgg cacgtcggcg ggaacagcaa gtggtccggc
     3121 gcgtacgagg gcaaggccgc ggtcctcgag ttcttcggtc gtcagacggg ggcgatggga
     3181 ggccagccgc cctcggtgga gatccacgac gtcgtcggcg gagacgatca cgtggtcgtc
     3241 ctggggaacg ccacggcgac gtcgccggac ggtcagagcg cggagtggaa gtacgcgcag
     3301 atcttccaca tcagggacgg gaaggcgacc gaggtctggg gcatggcgga gaacgacgcc
     3361 gacgtcgacc ccttcctgga cgccctcccc ggctgaccac gggggtcgcc cccggacgag
     3421 gcgacctccc ccgggatgac cggatcagtc gatcagcgcc tggaccgtcc ggatcgtgtc
     3481 ggtcaggcgc tcggccagca ggcgggcgaa cagccggtgg agggccgcgg ccgcgaaggg
     3541 gtcccgctgc tccatctcgt ggagtcggtc ccgggtgagg cggtgcagga cgctgggccg
     3601 ctcggccacc acggtggcgg agcggacgcc ccccgcgtag agcgctacct cc
//
LOCUS       DASUTW010000253         6384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1116792, whole genome shotgun sequence.
ACCESSION   DASUTW010000253 DASUTW010000000
VERSION     DASUTW010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116792"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VF058_08620"
     CDS             complement(<1..607)
                     /locus_tag="VF058_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6208403.1"
                     /translation="MVGVLLVDPEPFFGEALSAAIHGQDGVEVLGRSTDEREALRMCA
                     QRSPDVVLTEVALDAGSGLNLARRLGGDTRVVVLTRRPEGDVLLDSVDAGAVGCLGHD
                     LSVERLLQHLRDVVDRGRFAVNPDSLHETLRRATATAAGDALGVGRLTPREREVLSLV
                     ARGLDNARIANALYLSPHTVRTHVGNILRKLEVHSRAEAARV"
     gene            659..1618
                     /locus_tag="VF058_08625"
     CDS             659..1618
                     /locus_tag="VF058_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208404.1"
                     /translation="MSDRETDAAGATALEEQRRALEEQRRALDDAPWSYRGPRLHALG
                     FDFGIRANDGEVGAYLGGLFEAFEADGEPDHVYSFIARPEEEMRYSLYFDGEHVVSSR
                     EAGGALRYFQWDVNERVIARNRGYILIHAASAADETGRGIVMPAAMDSGKTTLVSGLV
                     RAGLRYLSDEAAAVDPRTGVLHAYAKALTIDPGSWDVLPDLRPSLEGRLAPFAQDQWY
                     VPADSIRRGAVASSARPAFVIAPRYVGEGRTELLPLSRAQALQTLVENAFNFRLFDGR
                     MALTVLADLVRGAECYRLMVAELPEACDLVLELVEASGEGGTE"
     gene            1615..1998
                     /locus_tag="VF058_08630"
     CDS             1615..1998
                     /locus_tag="VF058_08630"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX6208405.1"
                     /translation="MSEEKLQPKDVEPEVAPRPRATVAHVEIDGEGVLYDEVSNQIHL
                     LNPIATLIWSGLDGRTTLEELSAELSDAFGADLDRVRFDVLEAVRGFAEEDLLEDRGA
                     PPPEDAEAAQEASDEPRYLKDPPSP"
     gene            1999..2811
                     /locus_tag="VF058_08635"
     CDS             1999..2811
                     /locus_tag="VF058_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208406.1"
                     /translation="MLSIDRLVWTATRTLEVGSLWIGVRVSSDDVERAVAAAFGPYLV
                     EGLEPVPNFSVRFEDATDEQGVRYFQILYRGDCQVVRTMHRSRVIRALRSSVARFAGE
                     GSGLFRLRQVVLVSSGRAMLIPPDLLPRVEEIEADLRERGIEVLDPAFAHLDPATGEV
                     VVPERVIPLDEDTLRALDADGDRPPLPPGAEGARLPVAHWAVIRRDREEALTRMDVFA
                     ALMPSIEEEADVPIADAMRAVAGALSTASVGTIYHQKGEELLRTLGGLPGAG"
     gene            complement(2872..3396)
                     /locus_tag="VF058_08640"
     CDS             complement(2872..3396)
                     /locus_tag="VF058_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208407.1"
                     /translation="MSDELEPQGIKRREFIRKAAVTGATVAWAAPVVRTLAGAPAFAQ
                     TATPQGKDFSYIALCISCNGSHCRIKIDLDDNGNKAQCETGDSFQTPGCSLSCGATSN
                     CPSNACADIQASSADGGATVTVTLPPGCSIPHDGVAVGKCGNPPENGECVETFVTGTD
                     GSGRTTVTFSMCDV"
     gene            complement(3572..3931)
                     /locus_tag="VF058_08645"
     CDS             complement(3572..3931)
                     /locus_tag="VF058_08645"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX6208408.1"
                     /translation="MDRELPRPEAGSGEPRSEGLTVPPSYVPHRVPTVSELEVGEGLI
                     LYVEEADLIHRLNASAALVWRLFDGVATAGQLSREIAEEYELDVDEVSIQIASLVGEL
                     DALGLVSGQPEAERPTE"
     gene            4112..6010
                     /locus_tag="VF058_08650"
     CDS             4112..6010
                     /locus_tag="VF058_08650"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEX6208409.1"
                     /translation="MPRSDDRSTDRGLPSRIVAHIHSDVPLVLLDATLILIAYLIPLA
                     LRFEGGIPGENWRAFWMVLPAIAGIHLAANFLFGLYGEMWRYASVEEARRVALAGIVA
                     GGLVIAGDMLMIDTLIPRERPIPLAVVMLGSILALIGFGTVRFQSRLFAFKRRTEPEE
                     HHRVLLVGAGQAGAMVSKDLRTNRHLGLRPVGFVDDDPRKIGLALQEVRVLGPREAIP
                     SLVERLRVDQVLLTIPSATSEVVRDVAALCEQADVTLKVLPSVRDIMGGKVAARDIRD
                     LRIEDLLGRSQADIDLEAVAAFVRHRRVLVTGAGGSIGAEICRQVAAFDPAALTLLDH
                     DETHLHDVLTELEGDVPIETALVDIRDRERVIRAMMRHRPQVVFHAAAHKHVPLLEIH
                     PEEAVLTNVMGTANVVDAAVAAHVERFVLISTDKAVKPVSVMGASKWLAEQIARSALG
                     NGSIFCAVRFGNVLGSRGSVIPTMFRQIARGGPVTVTHPAMSRYFMSIQEAVELVLQA
                     AALAEGGEVFTLEMGEPVNILDMAKRIIRLSGRVPGKDVRISIIGARPGEKLVEDVID
                     PDEELLSSAHPDIMVSRPVPPSRSALHAAIGELDVMVRDGRTGDLADHIKAVASGLPV
                     PLDAEAAS"
     gene            6007..>6384
                     /locus_tag="VF058_08655"
     CDS             6007..>6384
                     /locus_tag="VF058_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208410.1"
                     /translation="MTSRTIVVIHRLAMVAEGIAAALSRYPWLVPVGIATDTAEGERL
                     ARLADAVAIDPEVSGADLLARRLGRAGIRVVRLGEDGGDGVAVPLAAPVASLVAALVP
                     GGPVASERSALTDREREILQLVAQ"
BASE COUNT          905 a         2187 c         2314 g          978 t
ORIGIN      
        1 cgacgcgggc cgcctctgcc cgtgagtgca cctcgagctt cctcaggatg ttgcccacgt
       61 gggttcggac cgtgtggggg ctgagataga gggcgttcgc gatccgcgcg ttgtcgagcc
      121 ctcgggccac caggctcagg acctcccgct cgcggggggt gagccggccg accccgagcg
      181 cgtccccggc ggccgtcgcg gtggcgcgcc ggagcgtctc gtggagcgag tcggggttca
      241 ccgcgaaccg tccgcgatcg actacgtcgc ggaggtgttg gagcagtcgc tcgaccgaga
      301 ggtcgtgccc gaggcatccg acggcgccgg catccaccga gtcgaggagg acgtcgccct
      361 cgggtcgtcg agtcagcacg acgacgcgtg tgtcgcctcc gagccgccgg gccaggttga
      421 ggccggatcc ggcgtcgagg gccacctcgg tgagcaccac gtcgggggag cgctgggcgc
      481 acatccgcag ggcctcccgc tcgtcggtgc tgcggcccag gacctctacc ccatcctggc
      541 cgtggatggc ggcgctcagc gcctctccga agaagggctc gggatcgacg aggaggaccc
      601 ctaccatgtt cgacatcgta cagtgcaggg gttgggcctc tccccgacga ggaccgagat
      661 gagcgatcga gagaccgacg cggcgggcgc aacggcgctc gaggagcagc ggcgtgcgct
      721 cgaggagcag cggcgtgcgc tcgacgacgc cccgtggtcc taccgcggtc cgcgccttca
      781 cgcgctcggg ttcgacttcg ggatccgggc gaacgacggg gaggtcggcg cctacctggg
      841 ggggctgttc gaagccttcg aagcggacgg ggagcccgat cacgtgtact ccttcatcgc
      901 tcgacccgag gaggagatgc ggtactcgct ctacttcgac ggcgagcacg tggtcagctc
      961 ccgggaggcc ggcggggcgc tgcgctactt ccagtgggac gtgaacgagc gcgtgatcgc
     1021 ccggaaccgg ggctacatcc tgatccacgc cgcgtccgcg gcggacgaga cggggcgcgg
     1081 catcgtgatg ccggccgcga tggactcggg caagaccacg ctcgtctcgg ggctggtgag
     1141 agccggcctc cgatacctga gcgacgaggc ggcggccgtg gatccgcgga cgggcgtgct
     1201 ccacgcctat gccaaggcgc tgacgatcga tccagggtcc tgggacgtgc tgcccgacct
     1261 ccgtccctcg ttggaggggc ggctcgcgcc cttcgcgcag gaccagtggt acgtgcccgc
     1321 ggactcgatc cgacgcgggg ccgtggcgtc gtccgctcgg cccgccttcg tgatcgcccc
     1381 tcggtacgtg ggcgagggca ggaccgagct cctgccactg tcgagggcgc aggcgctgca
     1441 gacgctggtg gagaacgcgt tcaacttccg cctgttcgat ggaaggatgg cgctgacggt
     1501 cctcgccgat ctcgtgcggg gggccgagtg ctaccggctc atggtggcgg agctgccgga
     1561 ggcctgcgat ctcgtgctcg agctcgtcga ggcgtcgggc gaggggggga ccgagtgagc
     1621 gaggagaagc tgcagccgaa ggacgtcgaa ccagaggttg ctccgcggcc gagggcgacg
     1681 gtggcccacg tcgagatcga cggcgagggt gtgctctacg acgaggtgag caaccagatc
     1741 cacctgctga acccgatcgc gaccctgatc tggagcggac tggacgggag gaccaccctg
     1801 gaggagctct cggctgagct gagcgacgcc ttcggcgccg acctggaccg cgtccgcttc
     1861 gacgtgctcg aggcggtccg ggggttcgcc gaggaggatc tgctcgaaga ccggggggca
     1921 ccccctcctg aggatgcgga ggcggcccag gaggccagcg acgagccacg gtacctcaag
     1981 gaccccccca gcccatgaat gctcagcatc gaccgtctgg tctggacggc aacgaggacc
     2041 ctcgaggtcg gatcgctctg gatcggggtc cgcgtcagct cggacgacgt cgagcgggcg
     2101 gtggcggcgg ccttcgggcc ctacctggtc gaaggcctcg agcccgtgcc gaacttctcc
     2161 gttcggttcg aggacgccac cgatgagcag ggcgttcggt acttccagat cctgtaccgg
     2221 ggtgactgcc aggtcgtccg gacgatgcat cggtcgcgcg tcatccgcgc cctccggtcc
     2281 tcggtcgcca ggttcgcggg cgaggggtcc ggtctcttcc ggctgcgcca ggtggtcctc
     2341 gtctcgagcg gccgggcgat gctgatcccg cccgacctcc tgccgcgggt ggaggagatc
     2401 gaggccgacc tgcgcgagcg ggggatcgag gtcctggatc cggcgttcgc ccacctcgac
     2461 cctgccaccg gcgaggtggt ggtcccggag cgggtcatcc ccctggacga ggatacgctg
     2521 cgggccctcg acgcggacgg agaccgtccg ccgctcccgc cgggcgcgga gggggcccgc
     2581 ttacccgtcg cgcactgggc cgtcatccgc cgggaccgcg aggaggccct cacccggatg
     2641 gacgtgttcg ccgcgctgat gccctcgatc gaggaggagg ccgacgtccc gatcgccgac
     2701 gccatgcggg ccgtggcggg agccctctcc acggcctcgg tggggacgat ctatcaccag
     2761 aagggcgagg agctgctgcg gaccctcggc ggtcttccag gggccggctg accccggaca
     2821 caaccgaggg cccgacgccg gcgccgggcc ctcggtcgat ctaccgggtc cttacacgtc
     2881 acacatgctg aacgtgaccg tcgtgcggcc ggagccgtcc gtgcccgtca cgaacgtctc
     2941 gacgcactcc ccgttctcgg gtgggttccc gcacttgccg acggcgacgc cgtcgtgcgg
     3001 gatcgagcac ccgggcggga gcgtcacggt gaccgtggcg ccgccgtccg ccgaggacgc
     3061 ctggatgtcc gcgcacgcgt tcgacgggca gttcgacgtg gccccgcagc tgaggctgca
     3121 gcccggggtc tggaagctgt ccccggtctc gcactgggcc ttgttgccgt tgtcgtccag
     3181 gtcaatcttg atccggcagt gcgacccgtt gcagctgata cacagcgcga tgtaggagaa
     3241 atccttcccc tgcggggtgg ccgtctgggc gaaggccggc gcgcccgcca gcgtccgcac
     3301 gaccggggcc gcccacgcga ccgtcgcacc cgtcaccgcg gccttgcgga tgaactccct
     3361 ccgcttgatg ccctggggtt cgagctcgtc actcattccc tgactcctct cgttcctctg
     3421 gtctggatcg tagaggcggt ggagccgcga acgcgatacg cggtcgggcg taagtccgct
     3481 cgccacgacg gggaagcccc ccgagcccag cgccacaggt cagaggggtg acggatgggg
     3541 tctctacgat gcggagcgta ggggcgtctg gtcactcggt cgggcgttcg gcctccggtt
     3601 gcccggaaac cagccccagc gcgtcgagct cgccgaccag cgacgcgatc tggatcgaca
     3661 cctcatcgac gtcaagctcg tactcctccg cgatctcccg cgagagctgg ccggcggtgg
     3721 ccactccgtc gaagagccgc caaacgagtg cggccgacgc gttgagcctg tggatcaggt
     3781 cggcctcctc gacgtagagg atcagcccct cgcccacctc gagctcggat acggtgggca
     3841 cgcggtgcgg cacgtacgag gggggtacgg tgagcccctc cgacctgggc tcgccgctcc
     3901 cggcctccgg acgcgggagt tctctgtcca cgacgggcta gtgtcgggcc ggacgggccg
     3961 gagggcatcc cgcaggaagc gtagatatga cgaccggacc cgtactgcat caggtgtaga
     4021 gaagatacga gacccgaccg atgaggtacg cagaccctcg ccctacgctg ttcgaccgat
     4081 gccagggaga tcgcccgcgg gggaggaagg ggtgcctcgt tccgacgacc ggtcgacgga
     4141 ccggggactt ccttcgagga tcgtcgctca catccacagc gacgtcccgc tcgtgctcct
     4201 cgacgcgaca ctcatcctga tcgcctatct cattccgctc gctctccgat tcgagggtgg
     4261 gatccccggc gagaactggc gcgccttctg gatggtgctc ccggcgatcg ccgggatcca
     4321 cctcgctgcc aacttcctct tcggtctcta cggggagatg tggcggtacg cgagcgtgga
     4381 ggaagcgagg agggtggccc tggccggcat cgtggccggt gggctcgtca tcgcgggaga
     4441 catgctgatg atcgacaccc tgatcccccg ggagcggcct atccctctcg cggtcgtgat
     4501 gctgggctcg atcctggcgc tgatcgggtt cgggaccgtc cggttccaga gccgtctctt
     4561 cgcgttcaag cgacggaccg agcccgagga gcatcaccgc gtgctgctgg tcggggctgg
     4621 ccaggcgggg gcgatggtct ccaaggacct ccggaccaac cggcaccttg ggctccgtcc
     4681 cgtggggttc gtggacgacg acccacggaa gatcggactg gctctccaag aggttcgcgt
     4741 cctcggcccc cgtgaggcca tcccctcgct ggtcgagcga ctccgggtgg accaggtgct
     4801 gctcacgatc ccgtcggcga ccagcgaggt cgtccgcgac gtcgccgcgc tgtgcgagca
     4861 ggccgacgtg accctcaagg tcctgccctc ggtccgagac atcatgggcg ggaaggtggc
     4921 ggcccgcgac atccgcgacc tgcggatcga ggaccttctg ggccgcagtc aggccgacat
     4981 cgacctcgag gccgtcgcgg ccttcgtgcg gcaccggcgg gtgctcgtga cgggcgccgg
     5041 cggctcgatc ggcgcggaga tctgccgcca ggtcgcggca ttcgatcccg cggcgctgac
     5101 cctcctcgac cacgacgaga cgcacctcca cgacgtgttg accgagctcg agggcgacgt
     5161 cccgatcgag acggcgctcg tggatatccg cgaccgggag cgggtgatcc gggccatgat
     5221 gcgccaccgc ccccaggtcg tcttccacgc ggccgcgcac aagcacgtgc ccctgctcga
     5281 gatccaccca gaggaggcgg tcctcaccaa cgtgatgggg actgccaacg tggtcgatgc
     5341 ggcggtggcg gcgcacgtcg agcgcttcgt cctcatctcg accgacaagg cggtcaagcc
     5401 cgtgagcgtg atgggggcgt cgaagtggct cgccgagcag atcgcccgca gcgctctggg
     5461 gaacgggagc atcttctgcg cggtccgctt cgggaacgtc ctggggagcc gggggagcgt
     5521 gatccccacg atgttccggc agatcgcccg gggcgggccc gtgaccgtca cccacccggc
     5581 gatgtcgcgc tatttcatga gcatccagga ggccgtggag ctcgtgctcc aggcggccgc
     5641 tctggccgag gggggagagg tgttcaccct cgagatgggg gagccggtca acatcctgga
     5701 catggccaag cgcatcatca ggctctccgg acgcgtcccc ggcaaggacg tccgaatctc
     5761 gatcatcggc gcgcgccccg gcgagaaact cgtcgaggac gtgatcgacc ccgacgagga
     5821 gctcctctcc agcgcgcacc cggacatcat ggtgtcgcgc ccggtccctc cgagccgctc
     5881 ggccctccac gcggcgatcg gtgagctcga cgtgatggtg agggatggga ggaccggcga
     5941 cctcgcggat cacatcaagg ccgtcgcctc gggcctcccc gtgcctctcg acgcggaggc
     6001 ggcgagttga cctcccgcac gatcgtggtg atccatcggc tcgccatggt ggccgagggc
     6061 atcgccgcgg ctctctccag gtacccctgg ctcgtgccgg tgggcatcgc caccgacact
     6121 gccgagggcg agcggctcgc gcggctcgcc gacgcggtgg cgatcgaccc ggaggtctcc
     6181 ggggcggatc tcctggcccg ccgcctgggc cgtgccggga tccgcgtggt gcggctgggc
     6241 gaagacggcg gcgacggggt cgcggtcccg cttgccgcgc ccgtggcctc gctcgtggcg
     6301 gcgctggtgc cgggaggtcc ggtggcctcg gagcgctccg cgctgaccga ccgggaacgc
     6361 gagatcctcc agctcgtcgc ccag
//
LOCUS       DASUTW010000254         6842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1246871, whole genome shotgun sequence.
ACCESSION   DASUTW010000254 DASUTW010000000
VERSION     DASUTW010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6842
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246871"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="VF058_08660"
     CDS             complement(<1..1280)
                     /locus_tag="VF058_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208411.1"
                     /translation="MRRAALAIVLGAILATTSPVAGRSSEARWIAYVRAGEQGGPGSL
                     IAVRPGGRGERTLLDTGVVGADAGGAGAVFALVRHGDTTELVRVRIGSGAADGPRATG
                     SLSGVAAVHGRVAVERRIASGSDVPKHLREAIDELASASITVLAPPRRPPGTTHTVAS
                     AGGRRYELRFTNDPDGVLSHAEQVNVFVGASPRPVAPLPDALPVEVRGTAGSFSCGAS
                     ACFLDWEEGSSYAVGEFGRPEDAIEFAESLRPMEDLVGPSWREGGAVAAPQLLVLGGP
                     EETVLESVEGFCECSFHPVDWDGAGERLLVVTRAEGFTTVTEYGRPDGPEVLHEAAGR
                     AILDASYTPAGVAMIESPAVGRPGPVRTIDGRTLARRAISFDVEGSTMAVVRGNGNVL
                     VRDLESGAERIVGTGAVSVTVSPGGVAPAPSEEAP"
     gene            1364..2005
                     /locus_tag="VF058_08665"
     CDS             1364..2005
                     /locus_tag="VF058_08665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6208412.1"
                     /translation="MFTDLAPDYDRLNTLLTLGRVRSWRRALVRAAGVRPGDRVLDVC
                     AGTGASLAAVGEAVGARGLAVGVDFTPAMLSRAEGVRVLGDALRLPFLDRSFDATVSA
                     FALRDVADQPACIAEMVRVTRPGGGVAILEVGRPRRQPMRAGFDLWFRGAVPRLGAAF
                     GQGESHRFLARSIAYLPEPQALLGMLRDAGLERVRAQDLTLGAARLFGGFRPA"
     gene            complement(1992..2150)
                     /locus_tag="VF058_08670"
     CDS             complement(1992..2150)
                     /locus_tag="VF058_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208413.1"
                     /translation="MSETRLPSGDPLSTRTRPEEGAWELRVIEDDLEMTLWSDEPVLI
                     EDTDLTRA"
     gene            complement(2378..2941)
                     /locus_tag="VF058_08675"
     CDS             complement(2378..2941)
                     /locus_tag="VF058_08675"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HEX6208414.1"
                     /translation="MKRVAILALALAACGEPAVTVVPEADLPQDVFGSPRPEALTELP
                     DSTIVYFVLNGRLIAVPRQLPAEAPSLPAAAVEALLQGPSSPWRTAIPEGTRVLSVEV
                     DGIVATVDLTDEFERSAPGHRPALRVAQVVYTLTETPPVEAVRFRIEGSPADVLTGRD
                     EVVPGSVTRANYQRFAPLEEDESGEAA"
     gene            complement(2938..4326)
                     /locus_tag="VF058_08680"
     CDS             complement(2938..4326)
                     /locus_tag="VF058_08680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6208415.1"
                     /translation="MARRRPRGLRRRIMVAFIGGALGVSALVAGSTYYLADRYLVNQR
                     IEAATEQSFSNIAFAVRYVEPEQASLAELAGLLEARGSFDVLLMKGEQWFPSTLSITQ
                     RSVPAPVLEEVRRGQVAYRFQRDEPFLVFGSPIPGSDITAFFFYPVREVAEALALLWK
                     VLLMVSLGAVMLAAIAAVRVSRRVVHPVRLASDAARRVAEGLLATRLPVAERDEIGTM
                     AHSFNEMAAALEERIARERRFVGDVSHELRTPLTTLRASADYLLERADELPEQHVRAV
                     ELLSADLDYLRRLVDDLLDISRLESGPIQLSLEPLNVGDLVREVVAQRSRVADGEVRV
                     EVDGGGDSLTTLADKRRLERVVGNLVDNALTHGGLPVTVRVGAANGTITLAVEDRGPG
                     IGGRDLPRIFDRFYKADPSRPRLDGRGSGLGLAFAQENAKLHGGEIEVESSPGAGATF
                     LFRLPRVSRTGT"
     gene            complement(4326..5015)
                     /locus_tag="VF058_08685"
     CDS             complement(4326..5015)
                     /locus_tag="VF058_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6208416.1"
                     /translation="MTGQLIVLVDDDARLREIVGMALEGEGYRVLPAGSAEEASDLLA
                     REEPDLLILDVMLPGQDGFELCRDVRTRSPVPILMLTAKTDTVDVVVGLESGADDYVT
                     KPFVTKELVARIRALLRRSRGPEDGNGQRRIAVGDLEILPDAASVTKGGEEIHLTKTE
                     FRLLTTMASRPNQVFTREMLLSQVWEYDYFGDSRIVDVHIRRLRSKIEADPRRPEIVQ
                     TVRGIGYKVVG"
     gene            complement(5309..5770)
                     /locus_tag="VF058_08690"
     CDS             complement(5309..5770)
                     /locus_tag="VF058_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX6208417.1"
                     /translation="MDPIREEHRHLLPHIESLRRAADLAPGDEARKVAAEAHRFLVEH
                     LLPHAEAEDRVLYPAVEEAMGASGAAATMSRDHVEVETLTRHLGGLLPRLEGDPGDAA
                     WAELRRILYGLHALVAVHFAKEEEIYLPILDARLSAEEGERIVHEMHGEHG"
     gene            complement(5812..6279)
                     /locus_tag="VF058_08695"
     CDS             complement(5812..6279)
                     /locus_tag="VF058_08695"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX6208418.1"
                     /translation="MATPPEEGFRPLPSHYDHCFGCGREHPTGLHLRMEGGGREVRGS
                     FTVTEHHQGAPMLAHGGVVAAAMDEGMGYLLYLLRTPAVTGRLEVDYRKPVPVGATLE
                     LRGILEKVEGRRIHARMTGHLGGDLAVEARAVYVEVGLDHFLPDRGLEAPYNP"
     gene            6402..6734
                     /locus_tag="VF058_08700"
     CDS             6402..6734
                     /locus_tag="VF058_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208419.1"
                     /translation="MRKGLWKLVAGGSAIGIGAAAGIAVAGTDAQAPQPVELGETRGT
                     STLDIEGTPVTVASPSPLGDSLDDGSAGASVDTSGSADTSGSADTSGTDTMDGADSPD
                     DDISGPGS"
BASE COUNT          923 a         2481 c         2517 g          921 t
ORIGIN      
        1 ggcgcctcct cggagggcgc cggagcgacc cccccagggc tcacggtgac ggagaccgcc
       61 cccgtcccga cgatccgctc ggcccccgac tccaggtctc tgacgagcac gttcccgttc
      121 ccgcggacga ccgccatggt cgatccctcc acgtcgaacg agatcgccct ccgggcgagg
      181 gtgcgcccgt cgatggtccg cacgggcccg ggccggccca cggcggggga ctcgatcatc
      241 gccacgcctg ccggcgtgta ggaggcgtcc aggatcgccc ggccggcggc ctcgtggagg
      301 acctccggac cgtccggacg cccgtactcg gtcaccgtag tgaacccctc ggccctggtc
      361 acgacgagca gccgctcgcc cgcgccgtcc cagtccaccg ggtggaacga gcactcgcag
      421 aaaccctcca cggactcgag cacggtctcc tcgggccccc cgagcacgag gagctggggc
      481 gcggcgaccg ccccgccctc gcgccacgac ggaccgacga ggtcctccat gggccgcagg
      541 gactccgcga actcgatcgc gtcctcgggt cggccgaact cccccaccgc gtagctcgag
      601 ccttcctccc agtcgaggaa gcaggcggag gctccgcacg agaaggagcc cgcggtaccg
      661 cggacctcca cggggagggc atcgggcagg ggggcgacgg gccgagggga ggcgccgacg
      721 aagacgttca cctgctcggc gtgggacagc acaccgtccg gatcgttcgt gaagcggagc
      781 tcgtagcgcc gcccgccggc gctggccacc gtgtgggtcg tccccggcgg ccttcgaggc
      841 ggggcgagca ccgtgatcga cgccgatgcg agctcgtcga tggcctcccg gaggtgcttc
      901 gggacgtcgg atccggacgc gatccggcgc tccacggcga cccggccgtg cacggccgcc
      961 acccccgaca gggatccggt ggctcggggg ccgtccgccg ctccggaccc gatccgcacc
     1021 ctgacgagct cggtcgtgtc gccgtggcgg accagggcga agacggcgcc cgcccccccc
     1081 gcgtcggctc cgacgacgcc ggtgtccagg agggtccgct cgcctctgcc gccggggcgg
     1141 acggcgatga gggagcccgg gccgccctgc tcccctgctc gcacgtaggc gatccaccgc
     1201 gcctccgagg accggcccgc caccggggac gtggtcgcga ggatggcgcc cagcacgatc
     1261 gcgagggcgg ctcgccgcac gccgccgagc gtacacgcta gggtgggggg tgtcgccgaa
     1321 ggatccggac atccgacccg acaggccgag gtcgatccgt gcgatgttca cggacctcgc
     1381 tcccgactac gatcggttga acaccctcct caccttgggc cgggtgcggt cgtggcgccg
     1441 agccctggtg cgggcggcgg gcgtccggcc gggggatcga gtgctcgatg tctgcgccgg
     1501 cacgggcgcc tccctggctg cggtcgggga ggccgtggga gcccgggggc tcgccgtggg
     1561 cgtcgacttc acgccggcga tgctttcgcg ggcggagggg gtgcgcgtgc tcggggacgc
     1621 cctccgtctg cccttcctcg accggtcctt cgacgccacg gtcagcgcct tcgccctgcg
     1681 cgacgtcgcg gaccagccag cctgcatcgc cgagatggtg cgagtgacgc gccccggcgg
     1741 aggtgtggcc atcctcgagg tggggcgccc ccgccgtcag ccgatgcggg cggggttcga
     1801 tctgtggttc cggggcgcgg tgccgaggct gggcgcggcg ttcgggcagg gcgagtcgca
     1861 caggttcctg gcccgctcca tcgcctacct gccggagccg caggcgctcc tggggatgct
     1921 tcgggacgcg gggctcgaga gggtgcgggc gcaggacctc accctgggcg ccgcccgcct
     1981 gttcggcggg ttcaggcccg cgtgaggtcg gtgtcttcga tcaggaccgg ctcgtcgctc
     2041 cagagcgtca tctcgaggtc gtcctcgatg acgcggagct cccaggcgcc ctcctccggc
     2101 cgggtacggg tagagagggg atcccccgaa gggaggcggg tctcgctcac gagacctcct
     2161 gccgccggag gagggccatc tgggatgtgc ccccggctcc tcggtcgcgc cgggacggac
     2221 cgcgcacgct catccgacga cccggcgcgg gggccgggac cggagacgct cccctgtgag
     2281 ggggtgggga cgaacgcccg atcctgcacg gcaccgagga tgcccccgag gcgtgtcgtt
     2341 cccataacgg atatatgacg gttctgtgac gatggcctca ggccgcttcg cccgactcgt
     2401 cctcctcgag cggcgcgaag cgctggtagt tggccctcgt caccgacccc ggcaccacct
     2461 cgtcccggcc ggtcagcacg tctgcggggc tcccctcgat ccggaaccgc accgcctcca
     2521 ccggaggcgt ctccgtcagc gtgtacacga cctgggccac gcgcagggcc gggcggtggc
     2581 ccggggcgct ccgctcgaac tcgtcggtga ggtccaccgt ggccacgatc ccgtcgacct
     2641 cgaccgacag cacgcgggtg ccctcgggga tcgcggtcct ccaggggctg gaggggccct
     2701 ggaggagggc ctccaccgcg gccgccggga gggacggagc ctccgccggc agctgtctgg
     2761 ggaccgcgat gaggcgcccg ttgagcacga agtagacgat cgtgctgtcg ggaagttcgg
     2821 tgagggcctc gggccggggg gatccgaaga cgtcctgcgg caggtcggcc tcgggcacga
     2881 ccgtcacggc gggctcgccg cacgccgcca gcgccagcgc gaggatggcg acccgcttca
     2941 tgtgcccgtc cgggagacgc gcgggagccg gaagaggaac gtcgcgccgg cgcccgggct
     3001 gctctccacc tcgatctcgc ccccgtggag cttggcgttc tcctgggcga aggccagccc
     3061 caagcccgag ccccgtccat cgagccgggg gcgggagggg tcggccttgt agaagcgatc
     3121 gaagatccga ggcaggtccc ggccgccgat cccgggtcct cgatcctcca ccgcgagggt
     3181 gatcgtgccg ttggccgcac cgacccggac ggtgactggg aggccgccgt gggtgagcgc
     3241 gttatcgaca aggttgccta ccacccgctc gaggcgtcgc ttgtcggcca gggtcgtgag
     3301 cgagtcccct ccaccgtcca cctcgacgcg cacctcgccg tccgccaccc gcgaccgctg
     3361 agcgaccacc tccctgacga ggtcccccac gttcagcggc tccagtgaga gctggatcgg
     3421 tcccgactcg agccgcgaga tgtcgaggag gtcgtccacg agcctccgca ggtagtcgag
     3481 atcggcggag agcagctcga cggcccgcac gtgctgctcg ggcagctcgt cggcccgctc
     3541 cagcaggtag tcggccgagg cgcgcagggt cgtcagcggt gtgcgcagct cgtgggagac
     3601 gtcgccgacg aagcgccgct ccctggcgat gcgctcctcg agggcggcgg ccatctcgtt
     3661 gaaggagtgg gccatcgtcc cgatctcgtc gcgctcggcg accggaagcc gggtggccag
     3721 gagaccctcc gccacgcggc gcgcggcgtc gctggccagc cggaccgggt gcaccacgcg
     3781 ccggctcacc cgcaccgcgg cgatcgccgc cagcatgacg gcgcccaggg acaccatgag
     3841 cagcaccttc cagaggaggg ccagggcctc cgccacttcc cgtaccgggt agaagaagaa
     3901 cgccgtgatg tctgacccgg ggatcgggga gccgaacacc aggaagggtt cgtcccgttg
     3961 gaaccggtag gcgacctgcc cccggcgcac ctcctcgagc acgggggcgg ggaccgatcg
     4021 ctgggtgatc gagagggtgg aggggaacca ttgctcaccc ttcatgagca gcacgtcgaa
     4081 ggacccgcgg gcctcgagca ggcccgccag ctccgccagg gacgcctgct ccggctcgac
     4141 gtaccggacg gcgaaggcga tgttcgagaa cgactgctcc gtcgccgcct cgatccgctg
     4201 gttcacgagg tagcggtcgg cgaggtagta ggtgctgccg gcgacgaggg ccgagacgcc
     4261 gagggccccc ccgatgaagg ccaccatgat ccgccgcctg aggccgcgtg ggcgccgccg
     4321 cgccatcacc ccaccacctt gtagccgatc ccgcggacgg tctgcacgat ctcgggccgc
     4381 cgcggatccg cctcgatctt ggaccgcagg cgccggatgt gcacgtccac gatcctggag
     4441 tcgccgaagt agtcgtactc ccacacctgg ctcagcagca tctcgcgagt gaacacctgg
     4501 ttgggcctgg acgccatcgt cgtgaggagc cggaactcgg tcttcgtgag gtggatctcc
     4561 tcgcccccct tggtgacgct ggccgcgtcc gggaggatct cgagatcgcc cacggcgatg
     4621 cgccgctgcc cgttgccgtc ctcgggccct cgcgagcggc ggagcagcgc tcggatcctc
     4681 gccacgagct ccttcgtcac gaagggcttc gtgacgtagt cgtcggctcc ggactccagg
     4741 cccacgacga cgtcgacggt gtcggtcttc gccgtgagca tcaggatcgg gaccggggag
     4801 cgggtgcgga cgtcccggca gagctcgaac ccgtcctgcc cggggagcat cacgtcgagg
     4861 atgaggaggt cgggctcctc gcgggccagg agatccgatg cctcctccgc ggagccggcg
     4921 ggcaggacgc gatagccctc cccttcgagc gccatgccca cgatctctcg gagacgagcg
     4981 tcgtcgtcca ccagcacgat gagctgcccg gtcacgggcc catgatgcca ccgccgggcc
     5041 gaccgggtgg agcccggtcg gcccggcggg acggggccct gctcctcgat gaacgcgggg
     5101 ccgagatgcc cgggcgccgg gcacccgccc gcccggtcac cgctccgccg aaaaagcgac
     5161 ccgaaaccgt taacgcagat gcggtacctg ccgataccag gggcgaggct ccccggcgtc
     5221 cgccggggac cgtggtgcgt ggcggcggct cgagtcgggt cccccgggtc gccgcccgca
     5281 cccatcttca gccgcgctcc cctgttcgtc agccgtgctc gccgtgcatc tcgtggacga
     5341 tccgctcgcc ctcctcggcg ctcagccgcg cgtcgaggat cgggaggtag atctcctcct
     5401 ccttggcgaa gtggaccgcc accagcgcgt ggagcccgta gaggatccgc cggagctccg
     5461 cccaggccgc gtcgcccggg tcgccctcca gccgggggag caggcccccg aggtggcgcg
     5521 tgagcgtctc gacctcgacg tggtcccggc tcatggtggc cgcggcgccg gaggcgccca
     5581 tcgcctcctc gaccgccggg tagagcacgc ggtcctccgc ctccgcgtgg ggcagcaggt
     5641 gttcgacgag gaagcggtgg gcctccgcgg cgaccttcct cgcctcgtcc cccggcgcca
     5701 ggtccgccgc ccgccggagg gattcgatgt gggggaggag gtggcggtgc tcctctcgga
     5761 tcggatccat cggttggctc atgggctctc gcctcctgtc cccaccgtac ctcaggggtt
     5821 gtagggggct tcgagtccac ggtcgggcag gaagtggtcc aggccgacct cgacgtagac
     5881 ggcgcgggcc tcgacggcca ggtcccctcc gaggtgcccg gtcatgcggg cgtggatcct
     5941 ccgcccctcg accttctcca ggatgccgcg gagctcgagt gtcgctccga ccgggacggg
     6001 cttgcggtag tcgacctcca ggcgcccggt gaccgccggc gtccgcagca ggtagaggag
     6061 gtatcccatg ccctcgtcca tcgccgccgc gaccacgccg ccgtgggcca gcatcggcgc
     6121 gccctggtga tgctcagtga cggtgaacga gccgcggacc tcgcggccgc caccctccat
     6181 ccgcagatgg aggccggtgg ggtgctcgcg cccgcagccg aagcagtggt cgtagtggga
     6241 ggggagcggg cggaacccct cctccggagg tgtcgccatg cgcggagacc ttaggtccgg
     6301 tatcccgcgc ggtcaacctg aaggaaccct caggccaggc tcaggggtgc ctcaggcggg
     6361 gcggcgacca tcgcttcaga caccgaccga aggaggtcag gatgcggaag ggattgtgga
     6421 agctcgtggc gggaggctcg gcgatcggga tcggcgccgc ggcggggatc gcggtcgccg
     6481 ggacggacgc gcaggctccc cagccggtcg agctgggcga gacgaggggg acctcgacgc
     6541 tcgacatcga ggggacgccg gtgacggtgg cctccccgag cccactcggc gactcgctgg
     6601 atgacgggag cgcgggggcg tcggtcgaca cctcggggag cgccgacacg tccgggtcgg
     6661 cggacacctc gggaaccgac acgatggacg gggccgactc gcccgacgac gacatcagcg
     6721 ggccgggcag ctgatgccgg agcgcggaat cccgcgggac caggcgctgc cgggcgccga
     6781 ggggctcacc gggcccctcg gcgcccgggc cgtggcgtcg ttcctggagg cgagggggtg
     6841 gc
//
LOCUS       DASUTW010000255         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1247413, whole genome shotgun sequence.
ACCESSION   DASUTW010000255 DASUTW010000000
VERSION     DASUTW010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247413"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..559)
                     /locus_tag="VF058_08705"
     CDS             complement(215..559)
                     /locus_tag="VF058_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX6208420.1"
                     /translation="MKAKEAAMIGRIWHGWTAPQNAEAYQRYLEDEILADIRSGREGF
                     KGTYVLRREDGDQVEFLTLALWDSMEAIQALVGDDHEAAYLPDEERRLLDRFEERVVH
                     YQVVLARVTDRP"
     gene            complement(869..1198)
                     /locus_tag="VF058_08710"
     CDS             complement(869..1198)
                     /locus_tag="VF058_08710"
                     /inference="COORDINATES: protein motif:HMM:NF024636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HEX6208421.1"
                     /translation="MTAEVEERSEAPAAARAPHEDEPIRRAKQRAEAIQGLYIHLLVY
                     LVINAGLFVINLLTKGDGGTWWFYWPVAIWGIALLIHVVTIFVPVFSPEWAERRAARM
                     LEEDRRF"
     gene            complement(1182..1958)
                     /locus_tag="VF058_08715"
     CDS             complement(1182..1958)
                     /locus_tag="VF058_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208422.1"
                     /translation="MSQATTTVRHAARGAETEPHTEGPGATRIFASVVGTLAALAGIE
                     HGVGEILQGSVPPESIVIESWPASEAFEILGGEPAMTVVPNLVATGILAIAVSVILGV
                     WAVGFADRRRGGLVLILLSLLLFLVGGGFGPPLIGVIAGIAATGIGAVPRRRPGTLGR
                     ALGTAWAWILGAAVLGYLGLVPGIPLLATFGGVESAALVAALGLFAFAALFLALAAAR
                     ARDRMVLPRESFSREAGWPVPASPGPTYGPGGGERHDGGG"
     gene            2447..>2719
                     /locus_tag="VF058_08720"
     CDS             2447..>2719
                     /locus_tag="VF058_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX6208423.1"
                     /translation="MFREKFWLSLVLTLPVVVWSAHIQELLGYRAPGFPGSDWIPPVL
                     GTAVFLYGGLVFLQAAVRELRARLPGMMVLIALAITVAFVFSWVVQL"
BASE COUNT          436 a          976 c          900 g          407 t
ORIGIN      
        1 gtggggcggg cccgttcccg tccgactccg ccggcccagt caagaccttt ccaggcggag
       61 acggggaccc gagcctgcgc agcgaggaga cgaaggatgc gctcgcgaat ctgatccctg
      121 ccggtaccga gctcctggag aagcaactca caacgaccga cccggcttag tagtgttcgt
      181 acgctgtagc cttcgcctcc gcaattgcgg ggggtcacgg acgatccgtg actctcgcaa
      241 gaacgacctg gtagtggacc acccgttctt cgaagcgatc gaggagccgt ctctcctcgt
      301 ccggtaggta ggcggcctcg tggtcgtccc cgacgagcgc ctggatggcc tccatggagt
      361 cccagagcgc cagtgttagg aactcgacct gatccccgtc ctcgcgtcgg agaacgtaag
      421 tgcctttgaa cccttccctc cccgatcgga tgtcggcgag gatctcgtcc tccaggtagc
      481 gttgatacgc ctctgcgttc tgcggggccg tccacccgtg ccatatccgt ccgatcatcg
      541 cggcctcctt cgccttcatc cccaacgtgg gtgagcggcc tgcggtcgca ccgcccgctc
      601 actcccaccg tccccggcga tgatcgcatc acgttccgtg gggccgagac aagcgaagag
      661 aggcggcatg taccgaccac cgcgaggcat gggggctggg cgatgaggtc atcgccgctc
      721 ccctcgggag atcaccgggg aaggcgggcc tcaggcagcc tcgaaggcaa aggagccgag
      781 ggtggccgcc cagatcgggg cgatcgcggc cgcgatcggc aggatcttcg tcggtccgaa
      841 gaatcccacc atcagcacct ggctggcctc agaaccgcct gtcctcctcc agcatccgag
      901 ccgcccgccg ctccgcccat tcgggcgaga acaccggcac gaagatcgtc accacgtgga
      961 tcagcagcgc gataccccag atcgccacgg gccagtagaa ccaccaggtc ccgccatctc
     1021 ccttggtgag caggttgatc acgaacagtc ctgcgttgat caccaggtag acgaggaggt
     1081 ggatgtacag gccctggatc gcctcggccc gctgcttcgc ccgccggatc ggttcgtcct
     1141 cgtgcggcgc ccgggcggcg gccggtgcct cgctccgttc ctcaacctcc gccgtcatgt
     1201 ctctcacctc cacccggacc ataggtgggc cccggcgacg cgggcacggg ccatccggcc
     1261 tcccttgaga aggactctcg ggggagcacc atccggtccc gggcccgcgc cgcggccagg
     1321 gccaggaaca gcgcagcgaa ggcgaacagt ccgagcgcag ccaccagggc ggcgctctcc
     1381 acgcctccga acgttgccag gagagggatg ccggggacca gcccgaggta tccaagcacc
     1441 gcggcaccca ggatccaggc ccaggccgtc ccgagggctc ggccgagggt ccccgggcgc
     1501 cgccggggaa cggccccgat gccggtggcg gcgatcccgg cgatgacgcc gatgagcggc
     1561 gggccgaagc ccccacccac caggaagagc agaagcgaca gcaggatcag caccagaccg
     1621 ccccgtcgcc gatccgcgaa cccgaccgcc cagactccca ggatcaccga gacggcgatc
     1681 gcgaggatcc ccgtcgccac caggttgggg acgaccgtca tggccggctc ccctcccagg
     1741 atctcgaagg cctccgaggc cggccacgac tcgatgacga tgctctcagg ggggaccgag
     1801 ccctggagga tctcgcctac cccgtgctcg atgcccgcca gcgcggctag cgtcccgacc
     1861 accgaggcga agatcctcgt ggcgccgggg ccctcggtgt ggggctccgt ctcggcccct
     1921 cgagccgcgt gtcgtacggt cgtcgtcgcc tgtgacatga ggatgcctcc tcccatgacc
     1981 cgatccatcg aaccgcagac cccgcgtggg gggtagggtc ggagagccgg cgagtcctgg
     2041 gtccttcggc gggccggtcc agccgaccgg cggatggccc tgccaggcac cgacgtaggg
     2101 cgccgggtgc tcgccgccga ctcgcccggg ccagagacgc ccgtgaacac cgtcagccgg
     2161 cgcttcggga tctcgatgct gacgtccttg aggttgttcc cgcgcgcgcc gtgcacgcgt
     2221 atcagatcct ggctgtcggc gacgtgcgac gcgggggact acgttcgcat gcgttgagac
     2281 tacggtgtca ggcaaggaca atgggactct cgccatgagc gaagccacgc cagaccacca
     2341 gggtcacgga accggcccgg acgccgacct cgcgacgcag gagcgagctg ggaaggcatc
     2401 ggcggagcac gagggccacg ataaacacga gggccacagc ccggagatgt tccgagagaa
     2461 gttctggctc tccctcgtcc tcaccctccc ggtggtcgtc tggtcggccc acatccagga
     2521 gctcctcggg taccgggcgc cggggttccc cggctcggac tggatcccac cggtgctggg
     2581 gacggcggtc ttcctctatg ggggcctggt cttcctccag gcggccgtgc gcgagctccg
     2641 ggctcgcctg ccggggatga tggtcctcat cgccctggcc atcaccgtgg ccttcgtctt
     2701 ctcctgggtg gtgcagctg
//
LOCUS       DASUTW010000256         4259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1636955, whole genome shotgun sequence.
ACCESSION   DASUTW010000256 DASUTW010000000
VERSION     DASUTW010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1636955"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VF058_08725"
     CDS             <1..978
                     /locus_tag="VF058_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX6208424.1"
                     /translation="SDDPAAEHRIKEINLAYQTLSDPARRRQYDMYGGEGFTPDMFDF
                     MGDVSDIFEMFFGSPWRGRRSRRPERGEDARLAIALDFEEAAFGVEKEIEVETLAPCE
                     ACDGSGREPGTEAVRCAHCGGNGEVSDVRRSVFGTVMTSRICSACGGTGQQIPSPCQS
                     CRGEGRVPRTKTITVEVPAGVADGVELRIEGSGHQGRRGTPPGDLYVGLRVRPHPVFE
                     RRGQDLVAVLEVPLTQAILGGEVEIETLDGPEVLKIPSGTKPGTVVRLGGRGVPHLGR
                     RGRGDLYVELDVALPGKLSKKERALVEQLAELRDEPTGRDAAPGRLRRP"
     gene            975..1742
                     /locus_tag="VF058_08730"
     CDS             975..1742
                     /locus_tag="VF058_08730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEX6208425.1"
                     /translation="MSLPHFFAGAPEPGDEVMLDRDDARHATRALRLRPGDRLTSSDG
                     RGGVAVARITEVGKDGVRALVEERRQVPPETPRLTVVLAAPKGERLTWAIQKLTEVGT
                     DRIVLAESGRSVRRWSGARAARARGRAEAIAREAAKQSRRARLPDLEGPTTWADALEG
                     AEEPLILLWEGATEGLAAVLPQEPPRALTLAVGPEGGITEEEARDAESRGAALAALGL
                     TILRTETAALAGAAIALSRYGRLGKRGASLDPGDASG"
     gene            1753..2097
                     /locus_tag="VF058_08735"
     CDS             1753..2097
                     /locus_tag="VF058_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX6208426.1"
                     /translation="MEDCLFCRIARGEQEADIVHAGEDVVAFRDINPQAPTHILIVPK
                     EHLESARAIRDRDGDLLVDVFHVAEHLAKAEGIERSGWRLVTNVGPDAGQSVHHLHFH
                     LLGGRPMAWPPG"
     gene            2250..3176
                     /locus_tag="VF058_08740"
     CDS             2250..3176
                     /locus_tag="VF058_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX6208427.1"
                     /translation="MVALLGERDEFLKIIESAFGSSIIVRGNEIAISGPPEESERVAV
                     LFEELLTLLERGQPLSPESVERTIDMIKRDEGRPSEVLTEVLRTARRKTIAPKTVGQQ
                     RYVDAIHRSTVTFAIGPAGTGKTYLAVAAAVRALQEKEISRIILTRPAVEAGERLGFL
                     PGTLYEKIDPYLKPLYDALYEMIEADTFQRSMERGTIEVAPLAYMRGRTLNDSFIILD
                     EAQNTSPEQMKMFLTRLGFGSRAVVNGDVTQVDLPSGQRSGLMVVEEILEGIEGIEFI
                     HLGARDVVRHKIVQDIVEAYRVFGEQQARASE"
     gene            3183..3626
                     /gene="ybeY"
                     /locus_tag="VF058_08745"
     CDS             3183..3626
                     /gene="ybeY"
                     /locus_tag="VF058_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX6208428.1"
                     /translation="MSDDPSSRPIVLISNRQDLPVDEQALARVARETLRAEGRPDVEL
                     SVSLVTRDEIADLHERYLGEPGPTDVLSFPQDDEAEDGVPLLGDVVVCPEVAAGQEGP
                     ESDVGLLLVHGILHLLGYDHETDEDRQEMWARQAGYAGVTMEGSS"
     gene            3632..>4259
                     /locus_tag="VF058_08750"
     CDS             3632..>4259
                     /locus_tag="VF058_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEX6208429.1"
                     /translation="MIVAVVLLVLFGSVLAMAEASISRMSRVRAIALEDEGRRNASRL
                     VMIETDPPRYLNAIYLWVMLAQNGSAILVAIIADRLYGSLGITLLSVAFTLVYFIIVE
                     AMSKTFGILHSDRVALALAPVVMGLSTVLAWPTRGLIGIANILLPGKGLRAGPFVSEE
                     EIRAMAEAGSEEGAIEEEEKELIHSIFEFGDTIVREVMVPRPDMVTIGK"
BASE COUNT          628 a         1480 c         1565 g          586 t
ORIGIN      
        1 agcgacgacc ccgccgccga gcaccgcatc aaggagatca acctcgccta tcagaccctg
       61 tcggacccgg cgcgtcgacg tcagtacgac atgtacgggg gcgaggggtt cacgcccgat
      121 atgttcgact tcatgggcga cgtctcggac atcttcgaga tgttcttcgg gagcccgtgg
      181 cgaggccgtc gatcccggcg tccggagcgc ggcgaggacg cccgcctggc gatcgcgctc
      241 gacttcgagg aggccgcctt cggcgtggag aaggagatcg aggtcgagac gctcgccccg
      301 tgcgaggcgt gcgacggctc gggtcgggag ccgggtaccg aggcggtccg ctgcgcccac
      361 tgcgggggga acggggaggt ctccgacgtg cgtcgcagcg tcttcgggac ggtgatgacg
      421 tcgaggatct gctcggcgtg cgggggcacg ggccagcaga ttccctcgcc atgccagagc
      481 tgccggggcg aggggcgcgt gccccggacg aagacgatca ccgtggaggt gccggcgggc
      541 gtggccgacg gcgtggagct ccggatcgag gggtccgggc atcagggaag gcgggggacg
      601 ccccccggcg acctctacgt cgggctccgc gtccggccgc atcccgtgtt cgagcggagg
      661 gggcaggacc tggtggccgt gctggaggtc ccgctcaccc aggcgatcct ggggggcgag
      721 gtcgagatcg agacgctcga cggtccggag gtcctgaaga tcccctcggg cacgaagccc
      781 gggaccgtgg tcaggctggg cggccgcggc gtcccgcacc tgggacgccg cggccgcggc
      841 gacctgtacg tcgagctcga cgtcgccctc cccgggaagc tctccaagaa ggagcgggcg
      901 ctcgtcgagc agctagccga gctccgggac gagcccaccg ggagggacgc ggcgcccggc
      961 aggctccgcc ggccgtgagc ctcccgcact tcttcgccgg agccccggag cccggcgacg
     1021 aggtgatgct ggaccgcgac gacgcccgtc atgccacgcg agccctgcgc ctccgccccg
     1081 gcgaccggct cacctcgagc gatgggcggg gcggggtagc ggtggcgcgc atcaccgagg
     1141 tggggaagga cggcgttcgg gccctggtcg aggagcgacg ccaggtcccc ccggagaccc
     1201 cgcggctgac cgtggtgctg gcggctccca agggggagcg gctgacctgg gcgatccaga
     1261 agctcaccga ggttgggacc gatcggatcg tgctcgcgga gtccggccgc agcgtccgcc
     1321 ggtggagcgg cgcgcgcgcc gcgagggcca ggggtcgtgc cgaggcgatc gcccgcgagg
     1381 cggctaagca gtcgcggcgc gcgcgcctcc ccgacctcga gggtcccacg acctgggccg
     1441 atgcccttga gggcgcggag gaaccgctga tcctcctctg ggagggcgcc accgaggggc
     1501 tggccgcggt cctgccccag gagccgcccc gggccctcac cctggccgtg ggccccgagg
     1561 gaggcatcac cgaggaggag gcccgggacg ccgagtcgcg gggcgccgcg ctcgccgccc
     1621 tcgggctgac gatcctccgg accgagacgg ccgccttggc cggcgcggcg atcgccctct
     1681 cgcgctacgg tcggctcggc aagcgcggcg cgtccctcga cccgggtgac gcgagcggat
     1741 agcatcgggc ccatggagga ctgcctcttc tgccgcatcg cccggggcga gcaggaggcc
     1801 gacatcgtcc acgcggggga ggacgtcgtc gcgttccgcg acatcaaccc gcaggccccc
     1861 acccacatcc tgatcgtccc caaggagcac ctggagtccg cccgcgcgat cagggatcgc
     1921 gacggggacc tgctcgtgga cgtcttccac gtcgcggagc acctggccaa ggcggagggt
     1981 atcgagcggt cgggctggcg gctggtcacg aacgtcgggc ccgatgcggg ccagtccgtc
     2041 catcacctgc acttccacct gctcggcggc aggccgatgg cctggccccc gggctgagcc
     2101 cccgcccgcg gcggccccca tggagcgcga gggagccagg ggtataatcg ccccgagggt
     2161 cgccggagcg accctcttcc caacccccca ggagcgagtt ggccgagagc accacccagg
     2221 tgaagatcct cgtccccggc gaccgatcga tggtcgccct cctcggcgag cgggacgagt
     2281 tcctcaagat catcgagagc gccttcgggt cctccatcat cgtgcgcggg aacgagatcg
     2341 cgatctcggg tcccccggag gagtcggagc gcgtcgccgt cctcttcgag gagttgctca
     2401 cgctgctcga gcggggccag ccgctcagcc ccgagtcggt cgagcggacg atcgacatga
     2461 tcaagcggga cgaggggcgg ccgtccgagg tgctgaccga ggtcctgcgc accgcccgcc
     2521 ggaagacgat cgccccgaag acggtgggcc agcagcggta cgtggacgcg atccaccgct
     2581 ccaccgtcac gttcgccatc gggccggccg gcaccggcaa gacctacctg gccgtggcgg
     2641 ccgccgtccg cgctctccag gagaaggaga tctcccggat catcctcacg cgcccggcgg
     2701 tggaggcggg cgagcgcctc ggcttcctcc ccgggaccct ctacgagaag atcgacccgt
     2761 acctcaagcc gctctacgat gccctctacg agatgatcga ggccgacacc ttccagcggt
     2821 cgatggagcg gggcacgatc gaggtggcgc ccctcgcgta catgcggggg cggaccctca
     2881 acgactcgtt catcatcctc gacgaggccc agaacacctc gcccgagcag atgaagatgt
     2941 tcctcacccg cctcggcttc gggtcgcgcg ccgtggtgaa cggcgacgtg acccaggtcg
     3001 acctccccag cggccaacgc tcggggctga tggtggtcga ggagatcctc gaggggatcg
     3061 agggcatcga gttcatccac ctcggggccc gcgacgtcgt ccggcacaag atcgtccagg
     3121 acatcgtgga ggcgtaccgt gtgttcggcg agcagcaggc tcgcgcgagc gagtgatcct
     3181 cgatgagcga cgacccctcc agtagaccga tcgtcctcat ctccaaccgt caggacctgc
     3241 cggtggacga gcaggcgctc gcgcgcgtgg cgcgggagac gctgagggcc gagggccggc
     3301 ccgacgtcga gctctcggtg agcctggtga cgcgggatga gatcgccgac cttcacgagc
     3361 gctacctggg cgagccgggc cccaccgacg tgctgtcgtt cccgcaggac gacgaggccg
     3421 aggacggcgt gcccctgctg ggcgacgtgg tggtctgccc cgaggtggcc gcggggcagg
     3481 agggccccga gtcggacgtg gggctgctgc tcgtccacgg catcctccac ctcctcggct
     3541 acgaccacga gaccgatgag gatcgccagg agatgtgggc ccggcaggcc ggatacgccg
     3601 gcgtcacgat ggaggggtcc tcgtgatctg gatgatcgtg gccgtcgtgc tcctcgtcct
     3661 gttcgggtcg gtcctggcca tggccgaggc ctcgatcagt cggatgagcc gggtgcgggc
     3721 catcgcgctc gaggacgagg ggcgtcgcaa cgcgtcccga ctcgtgatga tcgagaccga
     3781 cccgccccgc tacctcaacg cgatctacct ctgggtgatg ctggcccaga acggctcggc
     3841 catcctcgtg gcgatcatcg ccgaccgcct ctacggcagc ctggggatca ccctgctgtc
     3901 ggtggcgttc acgctcgtct acttcatcat cgtcgaggcg atgtcgaaga ccttcggcat
     3961 cctgcacagc gaccgcgtgg cgctggccct ggccccggtc gtcatgggcc tgtccacggt
     4021 gctggcctgg cccacgcgcg gcctgatcgg catcgccaac atcctccttc ccgggaaggg
     4081 cctgcgggcc ggccccttcg tctccgagga ggagatcagg gcgatggccg aggccggcag
     4141 cgaggagggc gcgatcgagg aggaggagaa ggagctgatc cactcgatct tcgagttcgg
     4201 cgacacgatc gtgcgcgagg tgatggtccc gcggcccgac atggtcacga tcgggaagg
//
LOCUS       DASUTW010000257         2990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_860279, whole genome shotgun sequence.
ACCESSION   DASUTW010000257 DASUTW010000000
VERSION     DASUTW010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860279"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VF058_08755"
     CDS             complement(<1..659)
                     /locus_tag="VF058_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6208430.1"
                     /translation="MRALGRDGMRPETRYAKSSGGYVAYQVFGDGPRDIVFVPSWSQN
                     LDAMWDEPTLVRYFQRLSRAGRVICFDKRGSGVSDPVPLANIPTLEEWMDDAVVAMDA
                     AGSEQAAVIGDAEGGPMAMLLAATVPDRVIALALVNTFARWSRAPDYPIGMPEGAFER
                     LMDIYEQTWGQNPDMLLLTAPSVADDPHIREWFVRYQRLSMPLGAATAMYRWVSSLDV
                     RA"
     gene            727..1038
                     /locus_tag="VF058_08760"
     CDS             727..1038
                     /locus_tag="VF058_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208431.1"
                     /translation="MKLGRRVPDDNLLPFVYGLMAIERRTLRWIWPRFIWVEGITGVW
                     IYFACFRIATPRWECWMFVALAFAGVIALIIRLLMGRRRLRMAEGSNLWGGPSLEDSS
                     V"
     gene            1547..2101
                     /locus_tag="VF058_08765"
     CDS             1547..2101
                     /locus_tag="VF058_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208432.1"
                     /translation="MLGRDTQRGRRLGRLLLSGAAVIALTAAACADGSSDSGGGTEAG
                     GAGGDSGDLTIVMDEYGFIPDTLNVSAGDEIEVTIVNDGNEPHELMIGMPMGGGPEWE
                     TDLFGMMDPEALSGEGYHMEGVEHEEEEEEHGMGHGAEVEVEPGGQVTLRMHVPEDAS
                     GEWEMGCYLPGHYEAGMHGTLVVS"
     gene            complement(2184..2588)
                     /locus_tag="VF058_08770"
     CDS             complement(2184..2588)
                     /locus_tag="VF058_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208433.1"
                     /translation="MDEFSFDVEGPSRPAGYPRPTAALPAGQAVLVTLQNDGGIVHEW
                     RVGEQVLADGGYEDDLLAMMEPQVLSGTGYRLVKHEEDAEAPGGLTVEVEPGASVTLR
                     LHVPADAAGTWEMGCFITGHYEAGMRAELRIE"
BASE COUNT          457 a         1008 c         1048 g          477 t
ORIGIN      
        1 gcccgcacgt cgagcgagga cacccaccgg tacatggccg tcgccgcccc caaaggcatg
       61 gagagccgct ggtaccggac gaaccactcc cggatgtggg gatcgtcggc gacgctcgga
      121 gcggtgagca ggagcatgtc cgggttctgc ccccaggtct gctcgtagat gtccatgagg
      181 cgctcgaagg cgccctcggg catgccgatc gggtagtcgg gggcgcggga ccagcgggcg
      241 aaggtgttca cgagcgcgag cgcgatgacg cggtcgggga ccgtcgcggc gagcagcatg
      301 gccatcggtc caccctccgc gtcgccgatg actgccgcct gctcggaccc ggcggcgtcc
      361 atggccacca ccgcgtcgtc catccactcc tccagcgtgg ggatgttcgc gaggggaacc
      421 gggtcggaga ccccggagcc gcgcttgtcg aagcagatca cgcgcccggc gcggctcagt
      481 cgctggaagt agcggacgag cgtgggctcg tcccacatcg cgtcgaggtt ctggctccag
      541 ctcgggacga agacgatgtc ccgcggcccg tcgccgaaga cctggtaggc gacgtacccc
      601 ccactcgact tcgcgtatct cgtttccggc cgcatcccgt cccttcccag cgccctcatg
      661 ctgacacgag ccgcgtggcc gctaccaacg ccgcgtagcc gggatctcgg tgcggtatgg
      721 gatgccatga agctgggcag gagggtcccg gacgataacc tcctcccgtt cgtgtacggg
      781 ctcatggcca tcgagcgccg gaccctgcgg tggatctggc cgcgcttcat ctgggtggaa
      841 gggatcaccg gtgtgtggat ctacttcgcc tgtttccgca tcgcgacccc ccgatgggag
      901 tgctggatgt tcgtggccct cgcgttcgca ggagtgatcg ctctcatcat ccggctccta
      961 atgggcagac gcagactgag gatggcggaa ggctccaacc tttggggcgg cccttccttg
     1021 gaagactcca gcgtgtagcg atcgatagtc ccgtggggcc cagtgacgac tgaatccgag
     1081 ccagttcgac cctcctcgat ccgaccgagg agtcgaccga cgagccgggg gcgtggccgc
     1141 ccggcgagcc ggggccgggg tgggggccga ccggcgcgcc ggagctgggg tcgacccaga
     1201 caccagctcg cgcagctccc gcgacagtcg ggggcaattg ccaatcgcag cgaagccctt
     1261 ccctctctgg tcgccccgct gcacgtcggc agtcgccttc tacatgagtt cgccgatcgt
     1321 cttcacgtcc tcagcgatcg cgcgccctct acccggtcgt cgagctgttc gcgctcgtcc
     1381 tcctgctcga cggcgtcttc gcgaccgcgt tcgtgttgct ggagcgtggg cgtttcgtct
     1441 cgggaaacga acgggcaaac ggcccggcgg aggcgggact tccgactctg cccatgatcc
     1501 gaggcggcgc ccaccctcgt cgataaccgt gcgagaggag agcgagatgc ttggacgaga
     1561 cacccaacga ggccgcagac tggggcggct cctgctcagc ggcgccgccg tcatcgccct
     1621 cacggccgcc gcgtgtgccg acggatcctc cgactccgga ggtgggacgg aggctggcgg
     1681 cgccggcggg gattcgggtg acctgacgat cgtcatggac gagtacgggt tcatcccgga
     1741 cacgctgaac gtctcggccg gagatgagat cgaggtcacg atcgtgaacg acggcaacga
     1801 gcctcacgag ctgatgatcg ggatgccgat gggcgggggt cccgagtggg agaccgacct
     1861 gttcggcatg atggacccgg aggccctgag cggtgagggg taccacatgg agggcgtgga
     1921 acacgaggag gaggaggagg agcacgggat gggtcacggc gccgaggtcg aggtggagcc
     1981 gggcggccag gtgaccctcc ggatgcacgt gcccgaggac gcctcgggtg agtgggagat
     2041 gggctgctac ctgcccggtc actacgaggc gggcatgcac ggaacgctgg tcgtgtcgtg
     2101 acgagtgacg ggcgcgctct gcgcgcccgt ctgcttctcc agcaacaggt tcccgggtgg
     2161 gccgtctcca ggacgaggat ctgctactcg atgcgcagct ctgctctcat gccggcctcg
     2221 tagtggccgg tgatgaagca gcccatctcc cacgtgccgg ccgcgtcggc cggcacgtgg
     2281 aggcgaaggg tgactgaggc gccgggctcg acctcgacgg tgagcccgcc cggtgcctcg
     2341 gcgtcttcct cgtgcttgac cagccggtat ccggtgccgc tcaggacctg cggctccatc
     2401 atcgccaaca ggtcgtcctc ataccctcca tccgcgagga cctgttcccc gaccctccac
     2461 tcgtgcacga tgccgccgtc gttctgcagt gtgaccagga cggcttgacc ggcggggagg
     2521 gcggcggtcg gtcgcgggta gcctgccggt cgagagggtc cctcgacgtc gaagctgaac
     2581 tcgtccatca gaagcaccag cggctgtggc tccggcgatt ccgtctgctg agactcggtc
     2641 gggctcgccg tctcggtggg acccgcggag tcgcagcctg tggccagccc agccaacaag
     2701 gccgccaccc cgatcaggga ggcgggtctg gtccatcctg cgccccatcc ccctgcagcc
     2761 acgacgtcac actaccatcc atccacccag ggctttccca tgccgccgag accgcggccg
     2821 aagcccccgc cgaggctgtt ccgggtttcg gaagtcgcgg gcgagccgcc cacagagccc
     2881 ggcggcgcgt tgacgggata ggagataagg ccctccgaaa cgtcgccccc taaacgcccc
     2941 gatccggtcg cggtcgctta cgcccggccc aggtcgggat gggatggggg
//
LOCUS       DASUTW010000258         2205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_471932, whole genome shotgun sequence.
ACCESSION   DASUTW010000258 DASUTW010000000
VERSION     DASUTW010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471932"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VF058_08775"
     CDS             <1..377
                     /locus_tag="VF058_08775"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006696738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX6208434.1"
                     /translation="FWPAPGSITSGFGWRVHPIFGTRRFHSGVDIDAGCGDPIWAAER
                     GTVISAGWSGGYGNATVIDHGDGLATLYAHQESIGVATGQRVGRGQLIGRVGTTGWST
                     GCHLHFEVRVNGTPVDPVPYLS"
     gene            411..1532
                     /locus_tag="VF058_08780"
     CDS             411..1532
                     /locus_tag="VF058_08780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX6208435.1"
                     /translation="MNQVRPVFIGALIVTVLAAGTFVAGTRLGDPRSSGWDVLEDTAD
                     ELQREAARPVSEQELVRAAIEGMLTVLDDPYAASLDPDQADGVRDLIDGSIIGIGVWL
                     RESPQGLRVTGVVEGGPAQRAGLAEGDVIVEAEGRSMEDVSVSDAAAVFTGKAGSRME
                     LVVERDGETRAVRVQRARIEIPDVEARMVEGAGYARLRQFGRGAADELGDAVRDLLDR
                     GAHGVVLDLRGNAGGLADEALDVADLFLDGGVVARIRERGKPERTERAGSEHLGSFPV
                     VVLVDGDTASASELVAGALQDRDRATLVGSTTFGKGSVLTVHEVSGDTTIQYTTAFFF
                     TPDGHAIEGQGVSPDVEVESSRGQDGDPQLDRALRVLGA"
     gene            1537..2001
                     /gene="smpB"
                     /locus_tag="VF058_08785"
     CDS             1537..2001
                     /gene="smpB"
                     /locus_tag="VF058_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX6208436.1"
                     /translation="MAEGQKTVATNRRARFDYEIDERYEAGIVLTGSEVKSLRGGKAS
                     LSEAYGRITDDEVWLEGLHIPPYAQDSTRREYDPRRPRKLLLRRVEIERLKGKLNERG
                     YTLVPMRVYFSHGLAKLEVGVGRGKRRYEKRQAIAEREHKREIEREMARRNR"
     gene            2035..>2205
                     /gene="ssrA"
                     /locus_tag="VF058_08790"
     tmRNA           2035..>2205
                     /gene="ssrA"
                     /locus_tag="VF058_08790"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          330 a          751 c          829 g          295 t
ORIGIN      
        1 tgttctggcc ggcgcccggc tcgatcacct cgggcttcgg gtggcgggtc catccgatct
       61 tcggcacgcg caggttccac tccggggtgg acatcgacgc cggctgcggc gatccgatct
      121 gggcggccga gcgcggcacg gtcatctcgg cgggatggag cgggggctac ggcaacgcca
      181 ccgtgatcga ccacggcgac ggcctggcca cgctctacgc gcaccaggag agcatcgggg
      241 tcgccaccgg gcagcgggtg ggccggggac agctgatcgg ccgcgtgggc accacgggat
      301 ggtccacggg ctgtcacctc cacttcgagg tccgcgtcaa cggcaccccc gtcgaccccg
      361 tgccctacct gagctgagaa cccgcggcga gcgggtaccc tgaaggcatc gtgaaccagg
      421 tccgccccgt cttcatcggc gcgctcatcg tcacggtcct cgcggccggg accttcgtcg
      481 ccggcacccg tctgggggat cctcgctcct cgggatggga cgtcctggag gacaccgccg
      541 acgagctgca gcgtgaggcc gcccgccccg tctccgagca ggagctggtt cgagcggcga
      601 tcgaggggat gctgaccgtc ctcgacgacc cctacgctgc ctcgctcgac cccgaccagg
      661 ccgacggggt ccgggacctg atcgacggca gcatcatcgg gatcggggtg tggctccggg
      721 agtcgcccca gggcctgcgc gtcaccgggg tcgtggaggg tggcccggcc cagcgggccg
      781 ggttggccga gggcgacgtc atcgtcgagg ccgagggccg ctcgatggag gacgtcagcg
      841 tgagcgacgc ggcggcggtc ttcaccggga aggccggatc gcggatggag ctcgtggtgg
      901 agcgcgacgg cgagacccgg gcggtccgcg tccagcgggc caggatcgag atccccgacg
      961 tcgaggcgcg catggtcgag ggggcgggct acgcgcgcct ccgccagttc ggacggggag
     1021 cggcggacga gctgggcgat gccgtccggg atctgctcga ccggggcgcc cacggggtgg
     1081 tgctcgatct gcgggggaac gcaggggggc tggcggacga ggccctcgac gtggccgatc
     1141 tgttcctcga cggcggcgtg gtagcccgga tccgcgagcg cgggaagccg gagcggacgg
     1201 agcgcgccgg aagcgagcac ctcgggtcgt tccccgtcgt ggtcctggtc gacggggaca
     1261 ccgcctcggc ctcggagctc gtggccggcg cgctccagga ccgggaccgt gccaccctcg
     1321 ttggctcgac caccttcggg aagggatcgg tgctgaccgt gcacgaggtc tcgggcgaca
     1381 ccacgatcca gtacacgacc gccttcttct tcacgcccga cggccacgcg atcgaggggc
     1441 agggggtgag ccccgacgtc gaggtcgagt ccagccgcgg ccaggacggg gatccgcagc
     1501 tcgaccgggc cctccgggtc ctcggcgcct gacgcgatgg ccgaggggca gaagaccgtc
     1561 gccaccaatc gccgagcccg cttcgactac gagatcgacg agaggtacga ggccgggatc
     1621 gtcctcaccg gctccgaggt gaagtccctc cgaggaggga aggcgtcgct gtccgaggcc
     1681 tacggtcgaa tcaccgacga cgaggtctgg ctggagggcc tccacatccc cccatacgcg
     1741 caggacagca cccgccgcga gtacgacccc cgccgccccc gcaagctcct gctgcggcgc
     1801 gtggagatcg agcgtctcaa ggggaagctc aacgagcgcg gctacacgct cgtgccgatg
     1861 cgcgtgtact tctcgcacgg tctggccaag ctcgaggtcg gcgtgggccg cgggaagcgc
     1921 cggtacgaga agcgccaggc tatcgccgag cgcgagcata agcgcgagat cgagcgcgag
     1981 atggcccgcc gcaaccgctg acctgcgccg atatacttgg aggcgaactg catagggggt
     2041 gaccggcttc gacccgggtg gagcaccgtc cgggggaagc gagccgaggt ccccggagtc
     2101 ctcgttaaag agccgggacc aagataagcg ccgattctga actcgcgctt gctgcctaaa
     2161 tagcagccgt ccgccgcgga gcgtctgcgg ccgcgggccg ggcgt
//
LOCUS       DASUTW010000259         4367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1766736, whole genome shotgun sequence.
ACCESSION   DASUTW010000259 DASUTW010000000
VERSION     DASUTW010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766736"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1785)
                     /locus_tag="VF058_08795"
     CDS             complement(<1..1785)
                     /locus_tag="VF058_08795"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6208437.1"
                     /translation="MAERALELQRKRHHDHLGVNHWMLALLERHGAMAEALVDGLEAE
                     LVRRQVRRRLDEGDSGGALELNDTVSRALQASGQEEASERYLASIVLAAAGYSVRAGS
                     VQGAVTPTQAQAGSRPGPPTPMLDEFGVDLTQQAREGKLPPVVGRESEIRLVCTTLCR
                     RTKRNPALIGPAGTGKTAIVEGLAQRVVAGQVPDPLKGIRIVAIQPSSLVAGAGIAGQ
                     FEERVKAVLKEAEGPGIVLFIDELHSIVGSGGLMGATDVGAQLKPALARGVSCIGATT
                     DDEYRRFIERDRAMERRFNPIAVEEMAREDTLMVLEAHRDVLGEMRGVEVADDVLHWL
                     LDFGEDFLRNRAFPDKAVDLLEQCVATAVMNGESKVDVPAARAVAEDMVRVPADLQAR
                     VSEVGRRLTEAALLKDQGVDELVARLSVTMRGLDLKPTRPNAVILLADQAAETSEELA
                     ETMAETLLGDSKRVVELDFGQFDRAEDVNTLLGAPPAYIGFGEHLPLHDLARMPWSVL
                     VCKNVDGCHPQVREVLAQALSDGFLTERSGKRVYLSDAVVVLTAPSEGAGRLSPGVLS
                     EEGTSSMERVLGARLLEQVDVVSTTRPVQ"
     gene            2030..2467
                     /locus_tag="VF058_08800"
     CDS             2030..2467
                     /locus_tag="VF058_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208438.1"
                     /translation="MVEKTRGKVKKFLQRSFGGAFREQGDILWGRQGTTVVMIRVLPW
                     PSLEDAQVQVIAGIARDVDPTVECLSFLLKENQDFILGGFALEGDRDIIFRHGIIGSK
                     MDQKELEASVLAVASTGDKYDEIITSRFGGVRISDLAGEPAAS"
     gene            complement(2633..>4367)
                     /locus_tag="VF058_08805"
     CDS             complement(2633..>4367)
                     /locus_tag="VF058_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013734229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX6208439.1"
                     /translation="ACNFTQKTLNAQAAGAIALVIYNNVGGSPFSPGGSAPGITIPTV
                     MVSQDDGNTIVAGLPATGTLIPSEGISNNQRVGVDSIAWGHEGGNDLTIASVDPGAAD
                     SPLSVDVSGDDITVNLGTDAGGALSSTAADVVAAINGDSEASALVFAYTYRGSSGSGI
                     AQPSPRRNLSDFLSAPESVSREPRTVKLLRIGKTRDGSKPGVFAYAQEHAREWVPPLV
                     AIETAERLLRNYESHGPTKQLVNRLDIFILPSTNPDGGEYSFHDFNSQRKNMTRHCAV
                     TGSYDLNARNSWGVDNNRNYTEYSGFDGYSGSSASCTSGTYRGPAELSEPESANIDWV
                     AGTFENIKFSMNLHSSGNYFMWSPGSYAMPGRVSAPRPTLAEESFFWGASSRILTEIK
                     QHRGLSVTPARTGPISDVLYSAAGNSGDMLWYKYGIYAWNFEVGTSFQPPFENPNPNG
                     GSAHAESQEFSNGLMELMRVAHDFDRDHERPVSSIEVTASATPGMVNVVFETTEPAAV
                     FYETDGSTPTYDSTLYASGGIREAGELLTVAEGTVFHFFSVDAAGNVENNYIPDGTRK
                     NFNKATAVLGG"
BASE COUNT          626 a         1544 c         1495 g          702 t
ORIGIN      
        1 ctggaccggc ctggtggtgc tcacgacgtc gacctgctcg aggagcctgg cgccgaggac
       61 ccgctccatc gaggaggtcc cctcctcgga gaggacgccc ggggagagac gtcccgcgcc
      121 ctcggagggc gcggtcagga ccacgacggc gtcgctgagg tacacgcgct tcccgctccg
      181 ctcggtcagg aacccgtcgg agagcgcctg ggccaggacc tcgcggacct gagggtggca
      241 gccgtccacg ttcttgcaca ccaggacgct ccagggcatc cgggccaggt cgtgcagggg
      301 caggtgctcc ccgaacccga tgtaggcggg gggggcgccc agcagcgtgt tcacgtcctc
      361 ggctcggtcg aactgtccga agtccagctc cacgacgcgc ttggaatccc cgaggagggt
      421 ctccgccatc gtctccgcga gctcctcgct cgtctcggcc gcctggtcgg ccaagaggat
      481 taccgcgttg ggccgcgtgg gcttgaggtc gagccctcgc atcgtgaccg agagcctggc
      541 gaccagctcg tccacaccct gatccttcaa gagggccgcc tcggtgaggc gccgtccgac
      601 ctcggacacc cgcgcctgaa ggtccgccgg cacccgcacc atgtcctcgg ccaccgcgcg
      661 ggcggccgga acgtcgacct tggactcgcc gttcatcacc gcggtggcga cgcactgctc
      721 gaggaggtcc accgccttgt ctgggaaggc ccggttccgc aggaagtcct ccccgaagtc
      781 gagcagccag tggaggacgt cgtcggccac ctccacgccc cgcatctccc ccagcacgtc
      841 gcggtgggcc tccagcacca tcagggtgtc ctcgcgggcc atctcctcca ccgcgatcgg
      901 gttgaaccgg cgctccatcg cccggtcccg ctcgatgaac cgtcggtact cgtcgtcggt
      961 ggtggcgccg atgcacgaga ccccccgggc cagcgccggc ttgagctggg cccccacgtc
     1021 ggtggccccc atcagcccgc cgctccccac gatcgaatgc agctcgtcga tgaagaggac
     1081 gatgcccggc ccctcggcct ccttgagcac cgccttcacc cgctcctcga actgcccggc
     1141 gatgccggcc cccgccacga ggctcgacgg ctggatcgcc acgatccgga tccccttgag
     1201 agggtccggg acctggcccg ccaccacgcg ctgggccaga ccctccacga tggcggtctt
     1261 gccggtgccg gccggcccga tcagagccgg gttcctcttt gtacggcggc acagcgtggt
     1321 gcacacgagg cggatctcgc tctcccgccc cacgaccggc ggcagcttcc cctcgcgggc
     1381 ctgttgggtg aggtcgacgc cgaactcgtc gagcatcggg gtcggcgggc cgggacggct
     1441 ccccgcctgg gcctgcgtgg gtgtcaccgc cccctgcacc gaccccgccc gcacgctgta
     1501 tccggcagcc gccaggacga tcgaggcgag gtaccgctcg ctcgcctcct cctgcccgga
     1561 ggcctggagg gcgcgcgaca cggtgtcgtt cagctcgagg gcgcctcccg agtccccctc
     1621 gtccagccgg cgacggacct gccgccggac cagctcggcc tcgaggccgt cgacgagcgc
     1681 ctcggccatg gcgccgtgac gctccagcag ggcgagcatc cagtggttca cgccgagatg
     1741 gtcgtgatgg cgcttccgct ggagctcgag ggctcgctcg gccatgcggc gggccccgcg
     1801 agtgagttgg agtgacccga cctcttctga cggtgccatc ggtcgtcccc tttcgccttc
     1861 tctcctcata cgaaagcgcc tggcgagggg gcgaggtagg gccgaaggtc ccggcctgtt
     1921 cggtcctcct cgatggggcc gtccggccct acggaacgtc ggaggcctca cctacgatga
     1981 ccctgcacgc gccagcgggt gcgcgcggag gtgcgttatg gatcccaggg tggtcgagaa
     2041 gaccaggggc aaggtgaaga agttcctcca gcggagcttc ggaggggcct tccgagagca
     2101 gggagacatc ctctgggggc ggcaggggac gaccgtggtg atgatccgcg tgctcccgtg
     2161 gccgtcgctt gaggacgcgc aggtccaggt gatcgccggg atcgcgaggg acgtcgatcc
     2221 cacggtcgag tgcctgagct tcctcctcaa ggagaaccag gacttcatcc tgggcgggtt
     2281 cgccctcgag ggcgatcggg acatcatctt ccggcacggc atcatcgggt cgaagatgga
     2341 ccagaaggag ctcgaggcct cggtgctggc cgtggcctcc accggggaca agtacgacga
     2401 gatcatcaca agccggttcg gcggggtgag gatcagcgac ctggcgggcg agccggcggc
     2461 gtcctgacga cgtcggcgag cttcctgcca accctccccg gaggggcgag cgagtaggac
     2521 ggaacgccca gtcgcgtcgc ccgcgccgac ttcggcctcc gcgcgaggtg agggggcgcg
     2581 gaggccgggg gatcctcccc ggcctccgcg ccccctcggc gctgcggcag gctcagcctc
     2641 ccagtacggc cgtcgccttg ttgaaattct tcctcgtacc gtccgggatg tagttgttct
     2701 cgacgttccc ggccgcatcg acggagaaga agtggaacac ggtcccctcc gcgacggtca
     2761 gcagctcgcc tgcctcgcgg atcccacccg aagcgtacag ggtggagtcg tacgtcggcg
     2821 tgcttccgtc cgtctcgtag aagaccgcag cgggctccgt ggtctcgaac accacgttga
     2881 ccatgcccgg cgtggcgctc gccgtcacct cgatcgagct caccggccgc tcgtggtccc
     2941 tgtcgaaatc gtgggccacc cgcatgagct ccatcagccc gttggagaac tcctgggact
     3001 ccgcgtgcgc ggatcccccg ttggggttgg ggttctcgaa cggcggctgg aacgaggtac
     3061 ccacctcgaa gttccaggcg tagatgccgt acttgtacca gagcatgtcc ccggagttcc
     3121 ccgccgccga gtacagcacg tcggagatcg ggcccgtcct ggccggggtc accgagagac
     3181 cgcggtgctg cttgatctcg gtgaggatcc gagaggacgc cccccagaag aaggactcct
     3241 cggccagggt cggccgcggc gccgagacgc gacccggcat cgcgtaggac ccgggcgacc
     3301 acatgaagta gttgcccgac gagtggaggt tcatcgagaa cttgatgttc tcgaaggtgc
     3361 ccgccaccca atcgatgttc gccgactcag gctcggacag ctccgccgga ccccggtacg
     3421 tgccgctcgt gcagctcgcg gacgacccgg agtacccgtc gaacccgctg tactcggtgt
     3481 agttccggtt gttgtccacg ccccacgagt tcctcgcgtt gaggtcgtag ctcccggtca
     3541 ccgcgcagtg gcgggtcatg ttcttccgct gactgttgaa gtcgtggaag gagtactcgc
     3601 ccccgtcagg gttcgtcgag ggcaggatga agatgtcgag ccggttcacc agctgcttcg
     3661 tcggaccgtg cgactcgtag ttccgcagca gccgctccgc cgtctcgatc gccacgagcg
     3721 ggggcaccca ctcacgcgcg tgctcctgcg cgtaggcgaa caccccgggc ttcgagccgt
     3781 cgcgggtctt tccgatccgc agcagcttca cggtccgcgg ctcgcgcgac accgactcgg
     3841 gcgcgctgag gaagtcgctc aggttcctcc gaggggaggg ctgcgcgatg ccgctgccgc
     3901 tgctgccccg gtaggtgtag gcgaatacga gcgcgctcgc ctcagagtcg ccgttgatcg
     3961 cggccaccac atcggcggcc gtgctcgaca gggctccgcc cgcgtcggtg cccaggttga
     4021 cggtgatgtc gtcaccggag acatccaccg acagcgggga gtccgccgcc ccagggtcga
     4081 cgctggcgat cgtgaggtcg ttgccgccct cgtggcccca ggcgatcgag tccacgccga
     4141 ccctctggtt gttgctgatg ccctcgctcg ggatcagggt cccggtggcc ggcaggccgg
     4201 cgacgatggt gttcccgtcg tcctgggaga ccatcaccgt cgggatcgtg atccccggcg
     4261 cggagccgcc gggagagaag gggctgccgc ccacgttgtt gtagatcacg agcgcgatcg
     4321 cgccggcggc ctgggcgttc agggtcttct gcgtgaagtt gcaggcg
//
LOCUS       DASUTW010000260         2632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_602944, whole genome shotgun sequence.
ACCESSION   DASUTW010000260 DASUTW010000000
VERSION     DASUTW010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602944"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VF058_08810"
     CDS             complement(<1..654)
                     /locus_tag="VF058_08810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX6208440.1"
                     /translation="MIGVVTDSAAGLPPELATRMGIEVVPLYLSVDGRSLRDGELPDL
                     YERLERKPGSVTTSMPAPADFEEAFARLPEGELVCVTLAASVSGLHGAARMAAEAGRK
                     RVEVIDSGGASMGEGWVALAAARAAAAGGSLEEVAAAARAAAERTRLVATIDTMEHLR
                     RSGRVGWIAALAATGLQLKPVFEMVRGEIRPVAKPRTRRRALDRVAAEALRDLEGGRA
                     "
     gene            748..1167
                     /locus_tag="VF058_08815"
     CDS             748..1167
                     /locus_tag="VF058_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6208441.1"
                     /translation="MTRSAERALAEVPLFEGLTRDQIRSLLDATVEVTCDAGQSMVDE
                     GMLAIDFYLILDGEAEVVSGGRHVASLGPGDHFGELAVIDGEPRSATVQATTRLRALR
                     LVADDFHRLLRTTPELAHRILVEAVRRLRSSPPPPTA"
     gene            1170..2435
                     /locus_tag="VF058_08820"
     CDS             1170..2435
                     /locus_tag="VF058_08820"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6208442.1"
                     /translation="MSPEERPARDERAGILGRGPFRMLLGGQAVSSLGDWVGTLAFIA
                     AAYELTGSPAAVGGVLVIRLLPPLLAAPLGGVLADRFDRRRVMVLTNLGMAVLISLAP
                     FAGLALLYAIAFSSEVLAMLFLPARDSTVPDLVPADSLPQANGLVLATSYGAIPFAAA
                     LFSGLRLLTPLPEQPLAVPFLFDALTFVVAAVVIARIPIPKRRGGADLRVVEGLGEAY
                     RFARRSPTVRSLAAGMGVAMLGGGVLFAVGIAYVRETLGGGDVEFGYLVSLWGLGMAA
                     GLGGVRLFVRRGEALAFRAAVATCGGALIAMALLPFLWLAFVGALVFGASFAVAIMLA
                     MTLIQRLDEELRGRLVGGAHMLFRVALAIGALGLGGLASGLESVSLVGVDLDGNQIGM
                     AGGGVLILLGAAAASRLTSPDGPPAPDPA"
     gene            complement(2395..>2632)
                     /locus_tag="VF058_08825"
     CDS             complement(2395..>2632)
                     /locus_tag="VF058_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006696691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX6208443.1"
                     /translation="DLRIQELLDRVGREGITEVILATNPNLEGNATAMYVAGLLKPLG
                     IRVTRLASGLPVGGDLEYADEVTLGQALEGRREM"
BASE COUNT          284 a         1015 c          926 g          407 t
ORIGIN      
        1 cgctcgaccc ccctccaggt cccgcagggc ctccgcggcc acgcggtcga gcgcgcgtcg
       61 ccgcgtcctc ggcttggcca cgggccggat ctctccgcgg accatctcga agaccggctt
      121 cagctggagg ccggtcgccg ccagggccgc gatccacccc acccggcccg accggcgcag
      181 gtgctccatc gtgtcgatcg tggccaccag ccgcgtccgc tcggcggcag cgcgggcggc
      241 ggcggccacc tcctccagcg accctcccgc ggccgccgcc cgcgccgccg ccaacgccac
      301 ccacccctcc cccatcgacg cgccgcccga atcgatcacc tcgacccgct tccgcccggc
      361 ctccgccgcc atccgcgccg ccccgtgcag ccccgacacc gaggccgcga gcgtgacgca
      421 cacgagctcc ccctcgggaa gtcgggcgaa cgcctcctcg aagtccgcgg gagccggcat
      481 cgaggtcgtc acgctcccgg gcttccgctc cagccgctcg tacagatcgg ggagctcccc
      541 gtcacggagg ctccgcccgt cgacgctgag gtacagcggc acgacctcga tccccatccg
      601 agtggccagc tcggggggca gccccgcggc gctgtcggtc acgaccccga tcatgaggtg
      661 attctcgtga gccgagggag cgagggccac ccgctagggc tatgcgggag ggccggagtc
      721 tcctacgatg cgggacgagg tgagcacatg acgcgaagcg ccgagcgggc gctggccgag
      781 gtccccctct tcgagggcct gacgagggac cagatccggt cgctgctgga cgcaacggtc
      841 gaggtcacgt gcgacgcggg ccagtcgatg gtggatgagg gcatgctggc catcgacttc
      901 tatctgatcc tcgacggcga ggccgaggtc gtctcgggcg gccggcacgt cgcgtcgctg
      961 gggcccgggg accatttcgg cgagctcgcg gtgatcgacg gcgagccacg ctcggcgacc
     1021 gtgcaggcca ccaccaggct gcgggcgctt cggctcgtcg ccgacgactt ccaccggctg
     1081 ctccggacga cacccgagct cgcgcaccgc atcctcgtcg aggcggtgcg ccggctccgg
     1141 tcgtcgccgc cgcccccgac ggcctgacgg tgtcgcccga ggagcgcccc gcccgggacg
     1201 agcgggccgg gatcctcggc cgaggcccgt tccggatgct cctgggcggg caggccgtct
     1261 cctcgctggg cgactgggtg ggcaccctgg ccttcatcgc ggcggcctac gagctcacgg
     1321 ggtctcccgc cgcggtgggc ggcgtcctcg tcatccgtct gctgccgccc ctcctggccg
     1381 cgccgctggg aggggtcctc gccgatcggt tcgaccgtcg ccgggtgatg gtcctgacca
     1441 acctgggcat ggccgtcctc atctcactgg cgccgttcgc tgggctcgcg ctcctctacg
     1501 cgatcgcgtt ctcctccgag gtcctggcga tgctgttcct gccggctcgg gactccaccg
     1561 tgcccgatct cgttccggcc gactcgctcc cccaggcgaa cggcctcgtc ctggccacct
     1621 cctacggcgc gatcccgttc gcggccgccc tcttctcggg cctccgtctg ctcacgcccc
     1681 tccccgagca gccgctcgcg gtgcccttcc tcttcgacgc cctcaccttc gtcgtggcgg
     1741 ccgtcgtgat cgcgcggatc cccatcccca agcggagggg tggggcggac ctccgcgtgg
     1801 tcgaggggct gggcgaggcc taccgcttcg cgcggcgatc gcctacggtc cgcagcctgg
     1861 ccgcgggcat gggcgtggcg atgctcgggg gcggcgtgct gttcgccgtc ggcatcgcct
     1921 acgtccggga gacgctgggc gggggcgacg tcgagttcgg ctacctcgtg tcgctgtggg
     1981 ggctggggat ggcggccgga ctggggggcg tccggctgtt cgtgcgcagg ggcgaggcgc
     2041 tcgccttccg cgccgccgtc gccacctgcg ggggggccct gatcgcgatg gccctgctcc
     2101 ccttcctctg gctcgcgttc gtcggagcgc tcgtcttcgg cgcgtcgttc gccgtggcga
     2161 tcatgctggc gatgacgctc atccagcggc tcgacgagga gctccgtggg aggctggtcg
     2221 ggggtgccca catgctgttc cgcgtcgcgc tcgcgatcgg ggcgctgggg ctcgggggcc
     2281 tagcctcggg cctggagagc gtttcgctgg tcggggtcga cctggacggc aaccagatcg
     2341 ggatggcggg cggaggcgtc ctgatcctgc tgggtgcggc cgcggcgagc cgcctcacat
     2401 ctcccgacgg ccctccagcg cctgacccag cgtgacctcg tcggcgtact cgaggtcgcc
     2461 ccccaccggg agcccgctgg ccaggcgcgt taccctgatc ccgagcggct tcaagagccc
     2521 cgccacgtac atcgcggtgg cgttcccctc gaggttgggg ttcgtggcca ggatcacctc
     2581 ggtgatcccc tcgcgcccca cccggtccag cagctcctgg atccgcaggt cg
//
LOCUS       DASUTW010000261         5597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1510047, whole genome shotgun sequence.
ACCESSION   DASUTW010000261 DASUTW010000000
VERSION     DASUTW010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510047"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1471
                     /locus_tag="VF058_08830"
     CDS             137..1471
                     /locus_tag="VF058_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX6208444.1"
                     /translation="MAGLRLLPDAPLNLDGYLAAGGGEGLRAAVEVPPEEVIERVRRS
                     GLRGRGGAGFPTGTKWRSVRDDEHTRKFLAVNGAEGEPGTFKDRWLMRKNPYAVLEGT
                     AIAAYAAGVEQAFIGVKAGFEREIERLGQAMGEMEGAGILGPTPIQVVPGPDEYLFGE
                     EKALLEVIEGNPPLPRIVPPYMEGLFRKPGEANPTVVNNVETLANVPFILREGPEAFR
                     SVGTEGSPGTMIFTISGDVRRPGVHELELGSSVYDLVFEVAGGPADGRKVKAVFPGAS
                     STVLSLEQIDTPLDFDSMRRVGSGLGSAGFVVYDDTACIVRTTLTYSRFLYVESCAQC
                     PACKHGTGQITELLERIDRGEGREQDLETILARAMTVTDAQRCALPTGETLIVQSAVQ
                     QFWEEFAAHLGRACESDREIPVPKYVDYDPQTGFALDERYHLKRPDWTYAEG"
     gene            complement(1468..2784)
                     /locus_tag="VF058_08835"
     CDS             complement(1468..2784)
                     /locus_tag="VF058_08835"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6208445.1"
                     /translation="MTAGRAAVALALALAACTSPSPPSPDPTAAPTPAPSPTADDAHV
                     LGVIASVTGPSRLADRPFLDGMALAEAELNGGGGVAGRPLRLEVVDDAGDAPAPARAL
                     SDMLRADGPEAILVVGEGRPVAALRPEVQARGRPVVLLGADLYSDRLLSRQVFQTSPP
                     LRWQARAVARYLSRDRGHRRIVAVQQPGDPARAAFLDELRREGVDVEATLRPGDGRLA
                     DRAEGADALIALVDLERLDAVLRAVDRLDDPPQLVLPADLLRTDLDGVPPGTVAPGPY
                     TWAPWARPLSRVRAFRTSFRRTIGRIPVASEQEGYDAVRILAAGLEAGRTGDGLVRFL
                     ERHRPTGPVYSSTSVTLGPDDHLFQGELWLGLYTVPGPGERPEPWLAGTPWRPIIRTF
                     TYTGQRTLFPERDRRVFFPFWRPGRPSPRFRRSRWGIVTGPEDPLH"
     gene            complement(2781..4259)
                     /gene="lysS"
                     /locus_tag="VF058_08840"
     CDS             complement(2781..4259)
                     /gene="lysS"
                     /locus_tag="VF058_08840"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007502298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX6208446.1"
                     /translation="MTTEDPREARRRKLEALRERGVAPFALRFDRSARAADLHEKHAG
                     LEADAETGEVVRVAGRLMTLRRHGRLSFATLRDGRGEIQLMLAEGTLSPDGLALLDDL
                     DLGDWVGAEGEVVTTKRGELSVRATELTLLTKALRPLPEKWHGLKDVEARYRQRYLDL
                     VANPEVAERVRQKARVLHAMRDHLDGLGFVEVETPVLQSVPGGGLAKPFLTHHDALDV
                     PMYLRIALELHLKRLLVGGLERVYEIGRTFRNEGVSTKYNPEFTMLEAYQALASYEDM
                     MELVEGLVKAAADAAIGTRRFTYRGTEIDLDAPWPRRTVIELVSEAVGEEVSLDTPAL
                     ELRRLAERHGIDPHATWGPGKLVLELFEKLVEESLIAPIIVKDFPREVSPLARPHRDD
                     PRLTEHFDLIMGGVELAPAYSELADPDEQRARFEEQAAQKAAGEEETHPFDEEFLVAL
                     EHGMPPAGGLGLGIDRLLVILTDAPSIREVILFPALRPQPPE"
     gene            complement(4256..5023)
                     /locus_tag="VF058_08845"
     CDS             complement(4256..5023)
                     /locus_tag="VF058_08845"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008746360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX6208447.1"
                     /translation="MLLAVDVGNTETVLGVFNDRELTRHWRISTVADRTADELALMFG
                     GLLEHQDLSFDRNVTAVVVASVVPSATSALREMVRRYFGFRPLIVEPGVKTGIPVLTD
                     NPKEVGADRVLNAVAAVELHGSPAVIVDFGTATTFDAVSDKGEYVGGVIAPGVQISAA
                     ALYEHTARLPRVELVAPSSVIGKSTVESVQSGIMYGYASMVDGVVERIAKELGEPTVI
                     ATGGLAPVMIEECRSIDHHEPWLTLQGLRIVFERNTG"
     gene            complement(5025..>5597)
                     /gene="nadC"
                     /locus_tag="VF058_08850"
     CDS             complement(5025..>5597)
                     /gene="nadC"
                     /locus_tag="VF058_08850"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX6208448.1"
                     /translation="ERTALNFLGRLSGVATQAARFVEAVGGTGVVVRDTRKTTPGLRS
                     LEKYAAALGGVTPHRAGLFDGILIKDNHVAAAGGVAEAVRAAKASRPDLRVECEVETP
                     EEAEAAVGAGAEEVLLDNMPLEAMREAVARVAGRARLEASGGVTLENARAVAETGVDA
                     LSSGAITHGAPWLDLSLEVLEDVDLPAEEG"
BASE COUNT          776 a         2003 c         2084 g          734 t
ORIGIN      
        1 tcgaggccac ccacgagcac gagcacaacc accccgcgct cgagcacgtc cacgaggcgc
       61 acgaggacgc ggagaaggag cacctgcggg aggcgcacat ccacgaccac gcctccccgg
      121 cacagtcccc ggcgtgatgg cggggctgcg gctcctcccc gacgcgccgc tcaacctcga
      181 cgggtacctg gccgccgggg gcggcgaggg cctgcgggcc gccgtggagg tcccgccgga
      241 ggaggtgatc gagcgggtgc gccgctcggg tctcaggggg cggggcggcg ccggcttccc
      301 caccgggacg aagtggcgct cggtccgcga cgacgagcac acgaggaagt tcctcgcggt
      361 gaacggcgcg gagggggagc cggggacctt caaggaccgc tggctgatgc ggaaaaaccc
      421 ttacgccgtg ctggagggca cggcgatcgc ggcctacgcg gcgggcgtcg agcaggcctt
      481 catcggggtg aaggcggggt tcgagcggga gatcgagcgc ctggggcagg cgatgggcga
      541 gatggagggg gcggggatcc tggggccgac cccgatccag gtggtgccgg gccccgacga
      601 gtacctcttc ggagaggaga aggccctcct ggaggtgatc gagggcaacc cgcccctccc
      661 ccgcatcgtc cccccctaca tggagggcct gttccggaag cccggggagg ccaaccccac
      721 cgtcgtgaac aacgtggaga ccctggccaa cgtcccgttc atcctgcgag aggggcccga
      781 ggcgttccgt tcggtcggga ccgaggggtc gcccgggacg atgatcttca cgatctcggg
      841 cgacgtgcgc cggccgggcg tgcacgagct cgagctgggg agctccgtct acgacctcgt
      901 cttcgaggtg gcgggggggc cggcggacgg ccggaaggtg aaggcggtgt tccccggggc
      961 gtcctcgacg gtactctcgc tggagcagat cgacacgccg ctcgacttcg actcgatgcg
     1021 gcgggtcggg tcggggctgg ggtcggccgg gttcgtcgtc tacgacgaca ccgcttgcat
     1081 cgtgcggacg accctgacgt actcgcggtt cctgtacgtg gagtcgtgcg cccagtgccc
     1141 cgcctgcaag catggcacgg gccagatcac cgagctgctg gagcggatcg atcgaggcga
     1201 gggccgcgag caggacctgg agacgatcct ggcccgggcg atgacggtga ccgacgccca
     1261 gcggtgcgcg ctcccgacgg gggagacgct gatcgtgcag agcgccgtcc agcagttctg
     1321 ggaggagttc gccgctcacc tcgggcgggc gtgcgagagc gaccgggaga tcccggtgcc
     1381 gaagtacgtc gactacgacc cgcagacggg gttcgcgctg gacgagcggt accacctgaa
     1441 gcggcccgac tggacgtacg ccgagggcta gtggaggggg tcctcgggcc ccgtgacgat
     1501 cccccaccgc gagcgacgga acctggggct gggtcgcccc ggacgccaga aggggaagaa
     1561 gactcggcga tcgcgctccg ggaagagcgt ccgctgaccc gtgtaggtga aggtgcggat
     1621 gatcggacgc cagggggtgc ccgcgagcca gggctccggg cgctcgcccg gccccggcac
     1681 cgtgtacagc cccagccaca gctccccctg gaagaggtgg tcgtcgggcc ccagcgtgac
     1741 cgaggtcgag gagtagacgg ggccggtggg gcggtgccgc tccaggaagc ggacgagccc
     1801 atcgcccgtg cggcccgcct ccagccccgc ggccaggatg cgcacggcgt cgtagccctc
     1861 ctgctcggag gccacgggga tccgtccgat ggttcggcgg aacgaggtgc ggaaggcgcg
     1921 gacacgggag agggggcggg cccacggcgc ccaggtgtag gggccggggg ccaccgtgcc
     1981 gggcgggacg ccgtcgaggt ccgtacggag gaggtccgcg gggaggacga gctggggcgg
     2041 gtcgtcgagg cggtcgacgg ctcggaggac cgcgtcgagc cgctcgaggt cgaccagggc
     2101 tatcagggcg tcggccccct cggcccgatc ggcgaggcgc ccgtcgccgg gacggagggt
     2161 cgcctccacg tcgacgccct cgcgccggag ctcgtccagg aacgcggccc gggccgggtc
     2221 cccgggctgc tggacggcca cgatccgccg gtggccgcgg tcgcgggaca ggtagcgggc
     2281 cacggcgcga gcctgccagc gcagcggggg gctcgtctgg aagacctgtc gggagaggag
     2341 gcggtcgctg tagaggtccg cccccaggag gacgacggga cgtcctcgcg cctgaacctc
     2401 gggtcggagg gcggcgaccg ggcgaccctc gccgaccacg aggatggcct ctgggccgtc
     2461 cgctcggagc atgtcggaga gggcccgggc gggggcgggg gcgtcgccgg cgtcgtccac
     2521 cacctcgagc ctgaggggcc tgccggcgac gccgccgccc ccgttgagct ctgcctcggc
     2581 cagcgccatg ccgtccagga agggccggtc ggcgagcctg ctggggccgg tcacggacgc
     2641 gatcaccccg agcacgtgcg cgtcgtccgc cgtggggctc ggagccgggg tcggggcggc
     2701 cgtcgggtcg ggcgacggcg ggctcgggct ggtgcatgcc gccagcgcca gcgcgagggc
     2761 gacggccgct cgtcccgcgg tcactcgggg ggctgagggc ggagggcggg gaagaggatg
     2821 acctcgcgga tcgagggcgc gtcggtgagg atcacgagga ggcggtcgat ccccaggccc
     2881 aggcccccgg ccggcggcat cccgtgctcc agagccacca agaactcctc gtcgaagggg
     2941 tgggtctcct cctcgccggc ggccttctgc gcggcctgct cctcgaaccg ggcgcgctgc
     3001 tcgtcggggt ccgccagctc cgagtacgcg ggcgccagct cgacccctcc catgatcagg
     3061 tcgaagtgct cggtcagcct ggggtcgtcc cggtgcggcc tggccagcgg cgacacctcc
     3121 cgagggaagt ccttcacgat gatgggggcg atcagcgact cctcgaccag cttctcgaag
     3181 agctccagaa cgagcttgcc cgggccccac gtcgcgtgcg ggtcgatccc gtggcgctcg
     3241 gcgagccgcc gcagctccag ggcgggggtg tccagcgaca cctcctcgcc gaccgcttcg
     3301 gacacgagct cgatcaccgt gcgccgcggc cacggcgcat cgaggtcgat ctccgttccc
     3361 cggtaggtga accgcctcgt cccgatcgcg gcgtcggccg ccgccttcac cagcccctcg
     3421 acgagctcca tcatgtcctc gtagctggcg agggcctggt acgcctcgag catcgtgaac
     3481 tcggggttgt acttcgtgga gacgccctcg ttgcggaagg tgcgcccgat ctcgtagacg
     3541 cgctccaggc cgccgacgag caggcgcttc aggtggagct cgagcgcgat gcggaggtac
     3601 atggggacgt cgagcgcgtc gtggtgggtg aggaagggct tggccaggcc cccgccgggc
     3661 accgactgga gcaccggtgt ctccacctcg acgaagccca gaccgtcgag gtggtcgcgc
     3721 atcgcatgga ggacgcgcgc cttctgacgg acccgctcgg cgacctccgg gttggccacc
     3781 agatcgaggt agcgctgccg atacctcgcc tcgacgtcct tcaggccgtg ccacttctcg
     3841 gggagcgggc ggagcgcctt cgtcagcagg gtcagctcgg tggcccggac ggacagctcc
     3901 ccccgcttcg tcgtcaccac ctcgccctcg gcccccaccc agtctccgag gtcgagatcg
     3961 tcgaggaggg ccagcccgtc cggcgacagc gtcccctccg ccagcatcag ctggatctcg
     4021 cccctcccgt cgcgaagcgt ggcgaacgag agcctcccgt ggcggcggag cgtcatgagg
     4081 cggccggcga cccgcacgac ctcgcccgtc tcggcgtcgg cctcgagacc ggcgtgcttc
     4141 tcgtggaggt cggccgcccg cgcggagcga tcgaagcgca gcgcgaacgg agcgacgcct
     4201 cgctcacgca gggcttccag cttgcggcgg cgcgcctctc gcggatcctc cgtcgtcatc
     4261 ccgtgttcct ctcgaagacg atgcgcagcc cctggagcgt gagccacggc tcgtgatggt
     4321 cgatcgagcg gcactcctcg atcatgacgg gcgccagccc cccggtggcg atcacggtcg
     4381 gctccccgag ctccttcgcg atccgctcca cgacgccgtc caccatcgag gcgtagccgt
     4441 acatgatgcc ggactgcacg ctctccacgg tgctcttccc gatcaccgag ctcggcgcga
     4501 ccagctccac gcgcgggagc ctggcggtgt gctcgtagag cgcggcggcc gagatctgca
     4561 cgccgggggc gatcacgccg cccacgtact cgcccttgtc agagaccgcg tcgaaggtcg
     4621 tggccgtccc gaagtccacg atcacggccg gggacccgtg gagctcgacc gccgccaccg
     4681 cgttcaggac ccggtcggcc cccacctcct tggggttgtc ggtgagcacg gggatcccgg
     4741 tcttcacgcc gggctccacg atcaggggcc ggaagccgaa gtagcggcgc accatctctc
     4801 ggagggccga cgtggcggac ggcaccacgc tcgcgacgac caccgcggtg acgttgcggt
     4861 cgaaggagag gtcctggtgc tcgaggaggc cgccgaacat cagcgccagc tcgtccgcgg
     4921 tgcggtcggc gacggtcgag atccgccagt gccgggtcag ctcgcggtcg ttgaacaccc
     4981 cgagcaccgt ctcggtgttg ccgacgtcca cggccagcag catctcagcc ctcctccgcg
     5041 ggcaggtcca cgtcctccag cacctcgagc gacaggtcca gccacggcgc tccgtgcgtg
     5101 atcgccccgg acgagagcgc gtccaccccc gtctccgcca cggcccgcgc gttctccagg
     5161 gtcacgcccc ccgaggcctc gagccgggcc cggcccgcga cccgggccac ggcctcccgc
     5221 atcgcctcca gcggcatgtt gtcgaggagc acctcctcgg ccccggcgcc caccgccgcc
     5281 tcggcctcct ccggcgtctc gacctcgcac tcaacgcgga gatcgggccg cgaggccttc
     5341 gccgcccgga cggcctccgc cacgcccccc gccgccgcca cgtgattgtc cttgatgagg
     5401 atgccgtcga acagcccggc acggtggggg gtcacccctc cgagcgccgc ggcgtacttc
     5461 tccagcgagc ggaggcccgg cgtggtcttc cgcgtgtcgc ggacgaccac gccggtgcct
     5521 ccgaccgcct ccacgaaccg ggcggcctgc gtggccacgc ccgagagcct ccccaggaag
     5581 ttgagggcgg tgcgctc
//
LOCUS       DASUTW010000262         2583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_212074, whole genome shotgun sequence.
ACCESSION   DASUTW010000262 DASUTW010000000
VERSION     DASUTW010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212074"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /gene="dnaG"
                     /locus_tag="VF058_08855"
     CDS             complement(<1..1224)
                     /gene="dnaG"
                     /locus_tag="VF058_08855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX6208449.1"
                     /translation="MARIRQDDVEAVRERTDIVRLIQQHVALKKAGRSFTGLCPFHQE
                     KSPSFTVDPGKGVYYCFGCGKGGNAFHFVMEVEQLTFPEAVERLAGPAGVTLRYEGGG
                     REDRRERSRRDALFRANDLAADLYHRMLMEGREGEEARRYLASRSITKETAERFRIGY
                     APGYPDFVLRRLGRELSPEILVQAGVAMKDQRGGVRDRFRARVTFPIADLSGRSVGFG
                     ARLLQGEGPKYLNSQETPVYRKGELLYNLDRAKGEIGKVGTAHVVEGYTDVIALAQGG
                     VETAVATCGTALSEGHFRLLSRFAARMVLAFDSDEAGARAAERAAGLFEAYPLEVAVL
                     VLPEGLDPADLVKERGADAFRELAEGAVPLVEYMLRRSLRDADLESPEGRSRAVRSAL
                     PIVAGLDDLVRREQYA"
     gene            complement(1240..2241)
                     /locus_tag="VF058_08860"
     CDS             complement(1240..2241)
                     /locus_tag="VF058_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX6208450.1"
                     /translation="MGSIRERQEDLERRTLSDRAALAAESKGREREEPAHHMRTAFQV
                     DRDRIVHSKAFRRLKHKTQVFISPEGDHYRVRLTHTLEVTQIARTIARALRLNEDLTE
                     AIALGHDLGHTPFGHLGEEALTPFLDRPFHHSEQSLRVVDHLERDGQGLNLTWEVRDG
                     ILGHPWKMPPPATLEGWTVRMADRIAYVNHDIDDAIRAGLLTEESLPRDVTAVLGAGH
                     SDRIEALVDDIVAQGVESGEVGMSPPIAEALDALRDFMFERVYLRPESRGEQEKAIGV
                     IRALFEHYLEHPEEVPAEYAEAPGGPATRVADYIAGMTDRYALRTYERLFLPQEWML"
     gene            complement(2283..>2583)
                     /locus_tag="VF058_08865"
     CDS             complement(2283..>2583)
                     /locus_tag="VF058_08865"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX6208451.1"
                     /translation="AYLVSQGIPDSAVDPSPVGRTSLESLEAASRYMEAEALQSAFLV
                     SDPWHNLRIRRMARDLGIEAYVSATFQSAARSQGTRLAGYVRETFAYVFYRLTGS"
BASE COUNT          352 a          929 c          928 g          374 t
ORIGIN      
        1 cgcgtactgc tcccgacgga cgaggtcgtc caggccggcc acgatcggga gggcgctccg
       61 gacggcccgc gagcgcccct ccgggctctc gagatccgcg tcccgcagcg agcggcgcag
      121 catgtactcg acgagcggca ctgccccctc cgccagctcc cggaacgcgt cggcccctcg
      181 ctccttcacg aggtcggcgg ggtcgaggcc ctcggggagg accaggacag ccacctcgag
      241 cgggtaggcc tcgaacagac cggcggcccg ctcggcggcg cgtgccccgg cctcgtccga
      301 gtcgaacgcc agcaccatcc gggcggcgaa gcgcgagagg agccggaagt gcccctccga
      361 gagggcggtc ccgcaggtgg ccacggccgt ctccaccccg ccctgggcca gcgcgatcac
      421 gtccgtgtag ccctccacca cgtgggccgt tccgaccttg ccgatctcgc ccttggcccg
      481 gtcgaggttg tagaggagct cgccctttcg gtagacgggg gtctcctggg agttcaggta
      541 cttggggccc tcgccctgga gcaggcgcgc cccgaacccc acgctgcgcc ccgagaggtc
      601 ggcgatgggg aacgtcaccc gggcccggaa ccggtcccgg acgccgccgc gctgatcctt
      661 catcgccacg ccggcctgca cgaggatctc gggcgagagc tcgcggccca gccgccggag
      721 gacgaagtcg gggtagccgg gcgcgtaccc gatccggaac cgctcggcgg tctccttcgt
      781 gatgctgcga gaggcgaggt agcggcgggc ctcctcgccc tcgcgcccct ccatcagcat
      841 ccggtggtag aggtcggcag cgaggtcgtt ggcgcggaag agggcgtcgc gccgcgagcg
      901 ctcccggcgg tcctcccggc cgcctccctc gtagcggagg gtgacgccgg ccgggccggc
      961 cagccgctcc acggcctcgg ggaaggtcag ctgctcgacc tccatcacga agtggaaggc
     1021 gttgccgccc ttgccgcacc cgaagcagta gtagacgccc ttccccggat ccacggtgaa
     1081 ggaggggctc ttctcctggt ggaaggggca gagccccgtg aacgaccggc cggccttctt
     1141 caaggcgacg tgctgctgga tcaggcggac gatgtcggtt cgctcccgga ccgcctcgac
     1201 gtcatcctgc ctgatgcgcg ccacgcgctc tcctccgcct cacagcatcc attcctgggg
     1261 aaggaagagc cgctcgtacg tccgcagcgc gtaccggtcg gtcatgccgg cgatgtagtc
     1321 ggcgacccgg gtggccgggc cccccggcgc ttcggcgtac tccgccggga cctcctcggg
     1381 atgctccagg tagtgctcga acagcgccct gatcaccccg atcgccttct cctgctcgcc
     1441 ccggctctcc gggcggaggt agacccgctc gaacatgaag tcgcggagcg cgtcgagcgc
     1501 ctcggcgatc gggggcgaca tccccacctc gcccgactcg acgccctggg ccacgatgtc
     1561 gtcgacgagg gcctcgatcc ggtcgctgtg cccggcgccg agcaccgcgg tgacgtcccg
     1621 agggagcgac tcctcggtga gcaggcccgc gcggatcgcg tcgtcgatgt cgtggttgac
     1681 gtaggcgatg cgatcggcca tgcggacggt ccagccctcg agggtggcgg ggggcggcat
     1741 cttccaggga tggcccagga tgccgtcccg gacctcccag gtcaggttca ggccctgccc
     1801 gtctcgctcc aagtggtcca cgacgcggag gctctgctcg ctgtgatgga acggccggtc
     1861 caggaacggc gtgagcgcct cctcgcccag gtgcccgaac ggggtgtgac cgaggtcgtg
     1921 gccgagggcg atggcctcgg tgaggtcctc gttgagccgg agggcccgag cgatggtccg
     1981 ggcgatctgc gtgacctcga gcgtgtgggt gaggcggacg cggtagtgat ccccctcggg
     2041 cgagatgaac acctgcgtct tgtgcttcag gcggcggaag gccttggagt ggacgatgcg
     2101 gtcccggtcg acctggaagg cggtccgcat gtggtgggcc ggctcctccc gctcccggcc
     2161 cttggactcg gcggcgagcg ccgcgcgatc cgacagcgtc cgacgttcca gatcctcctg
     2221 acgctccctg atcgagccca cgggccgaag ggtagcacga gcctccgacg cctcctccgg
     2281 gctcaggagc ccgtcagccg gtagaagacg tacgcgaacg tctcccgcac gtagccggcc
     2341 agccgcgtcc cctggctcct ggccgccgac tggaaggtgg ccgagacgta ggcctcgatg
     2401 ccgaggtccc gggccatccg gcggatcctg aggttgtgcc aggggtccga gacgaggaac
     2461 gcgctctgga gggcctcggc ctccatgtac cgggaggccg cctccaggct ctccagggag
     2521 gtgcggccga ccggcgaggg gtccacggcc gagtccggga tcccctggga cacgaggtag
     2581 gcc
//
LOCUS       DASUTW010000263         2927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1641111, whole genome shotgun sequence.
ACCESSION   DASUTW010000263 DASUTW010000000
VERSION     DASUTW010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641111"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..169
                     /locus_tag="VF058_08870"
     CDS             <1..169
                     /locus_tag="VF058_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-en-1-yl diphosphate
                     synthase"
                     /protein_id="HEX6208452.1"
                     /translation="GPGEAREADLGIAAGKGYGQLIVRGKVQEKRIPESEFVEALVAE
                     AKKLAAETPRA"
     gene            217..1926
                     /locus_tag="VF058_08875"
     CDS             217..1926
                     /locus_tag="VF058_08875"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX6208453.1"
                     /translation="MSELFAHTLREPPGDEEAPSARLLAQGAFVRKLMSGVFTMLPLG
                     LRVLRKVEGIVREEMDAKGAQEVRMPAILPSEPWKISGRWQAYAAENLMFVLHDRHDR
                     ELGLGPTHEEVVTPLVDADVSSYRDLPLNLYHIQWKYRDEARPRSGLIRAREFLMKDA
                     YTFDRDLDGLRVSYGKMVDAYRRIFTRCGLDFRVVEADPGLIGGDVNHEFMAPAEVGE
                     DLFVHCTSCDYAANVEAAEAHWPKAPAQEEPKPMEVVPTPGRATIEAVADLLQVPEHR
                     VLKCLVYKVKDELVAVLLPGDREVSENKLARLFAPEEVGMLTDADFEKAGLAKGYIGP
                     QGLEGVTVIADHSIRTGANWVAGANEPDAHVTGVNLDRDFAVDRWEDLSGVKEGDVCP
                     RCHEGTLMLGRSIEVGHTFQLGTRYSDPLEATFVDEDGSERPFLMGCYGIGVSRIISA
                     VVEQHLDEKGIAWPRAIAPYDVVLIPTNMDDEQVVAVAERLYGELTEAGIEVVIDDRD
                     ERAGVKFTDAELIGYPVAVVVGKRGIEAGALEVRFRATGDQREVPLNDAAAALPGLVA
                     EAP"
     gene            1969..2250
                     /locus_tag="VF058_08880"
     CDS             1969..2250
                     /locus_tag="VF058_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208454.1"
                     /translation="MAAKMRSEFRRFAMVANDPEAVIQRIGRDTWDCIVIDVGGTWAR
                     EVFPSQEAAEGACRALGVRHQIGWEDQRLAQRMNRRDHWGEPGGQRRAV"
     gene            2370..2822
                     /gene="rimP"
                     /locus_tag="VF058_08885"
     CDS             2370..2822
                     /gene="rimP"
                     /locus_tag="VF058_08885"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX6208455.1"
                     /translation="MDLETTIGPVVEGMGLELVEATFRKEGGRRVLRVTVDRDGGIDL
                     DTIAETSEKVSRRLDALDFGGDAAYTLEVSSPGVERPLRKPEDFAKRVGERVKVKTYA
                     PIDGERSHRGEIVAADEEAVTIRVEDAERRMAWDDIASARTIFEWGPS"
     gene            2819..>2927
                     /locus_tag="VF058_08890"
     CDS             2819..>2927
                     /locus_tag="VF058_08890"
                     /inference="COORDINATES: protein motif:HMM:NF020118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HEX6208456.1"
                     /translation="MNLEFLEALRELERERGISFEVLLHGMEEALASAWK"
BASE COUNT          448 a          945 c         1117 g          417 t
ORIGIN      
        1 cgggcccggc gaggctcgcg aggccgacct ggggatcgcg gccgggaagg gctacggcca
       61 gctgatcgtc cgggggaagg tccaggagaa gcgcatcccc gagtcggagt tcgtggaggc
      121 cctggtggcc gaggccaaga aactggccgc cgagacgccg cgagcctgat ccgggcggcc
      181 gggcgccgcc tccctgtatc ctgacggacc atgcggatgt ccgagctgtt cgcgcacacg
      241 ctccgggagc ctcccggcga cgaggaggct ccctcggcca ggctgctcgc gcagggggcc
      301 ttcgtccgga agctgatgtc cggcgtgttc acgatgctgc cgctcggtct ccgcgtcctg
      361 cgcaaggtcg aggggatcgt gcgggaggag atggacgcca agggagccca ggaggtgcgg
      421 atgccggcca tcctcccgtc ggagccctgg aagatatccg gccgctggca ggcctacgcc
      481 gcggagaacc tgatgttcgt cctccacgac cggcacgacc gcgagctcgg cctcggcccc
      541 acccacgagg aggtcgtgac cccgctcgtg gacgccgacg tgtcgtcgta ccgggacctg
      601 ccgctgaacc tctaccacat ccagtggaag taccgggacg aggcgcgccc tcggtccggc
      661 ctgatccggg cccgcgagtt cctgatgaag gacgcctaca cgttcgaccg cgacctcgac
      721 ggtcttcgcg tctcctacgg caagatggtg gacgcctaca ggcggatctt cacccggtgc
      781 gggctcgact tccgtgtggt ggaggccgac cccggcctga tcgggggcga cgtcaaccac
      841 gagttcatgg ctcccgccga ggtgggcgag gacctcttcg tccactgcac gagctgcgac
      901 tacgcggcca acgtcgaggc ggcggaggcc cactggccca aggcccccgc gcaggaggag
      961 ccgaagccga tggaggtggt ccccacgccg gggcgcgcca cgatcgaggc ggtggcggac
     1021 ctcctccagg tgccagagca cagggtgctg aagtgcctcg tctacaaggt gaaggacgag
     1081 ctggtcgccg tcctgctgcc cggcgaccgt gaggtcagcg agaacaagct cgcccggctg
     1141 ttcgctcccg aagaggtggg gatgctgacg gacgccgact tcgagaaggc gggcctggct
     1201 aagggctaca tcggtcccca gggcctcgag ggcgtgacgg tgatcgccga tcactcgatc
     1261 cggacgggcg cgaactgggt ggcgggggcg aacgagccgg acgcgcacgt caccggggtg
     1321 aacctcgacc gcgacttcgc ggtcgaccgg tgggaggacc tctcgggggt gaaggagggg
     1381 gacgtctgcc cccgctgtca cgaggggacg ctgatgctgg gtcgctcgat cgaggtcggg
     1441 cacacgttcc agctcgggac ccgctactcg gatcctctcg aggccacgtt cgtggacgag
     1501 gacggctccg agcgaccgtt cctgatgggc tgctacggga tcggcgtcag ccggatcatc
     1561 tcggccgtcg tggagcagca cctcgacgag aagggcatcg cctggccccg agccatcgct
     1621 ccgtacgacg tcgtgttgat ccccacgaac atggacgacg agcaggtcgt ggcggtcgcc
     1681 gagcggctgt acggagagct gaccgaggcc gggatcgagg tcgtgatcga cgaccgcgac
     1741 gagcgggccg gcgtgaagtt caccgacgcc gagctgatcg ggtacccggt cgcggtggtg
     1801 gtcgggaagc gggggatcga ggcgggggca ctggaggtcc ggttccgggc gaccggcgac
     1861 cagcgcgagg ttcccctgaa cgacgcggcc gccgcgctcc ccggcctcgt cgccgaggcg
     1921 ccctgagggc ggttgcgggt gggcccgacc tcccgtacgg ttccttcgat ggcggccaag
     1981 atgcgctccg agttccggcg cttcgcgatg gtggcgaacg accccgaggc cgtgatccag
     2041 cgtatcgggc gtgatacctg ggactgcatc gtgatcgacg tcggcgggac gtgggcccgt
     2101 gaggtcttcc cctcgcagga ggccgccgag ggggcctgcc gggcgctcgg cgtccgccac
     2161 cagatcgggt gggaggacca gcggctggcc cagcggatga accgccggga ccactggggc
     2221 gagccgggag gccagcgccg ggccgtctga ttcgagcggg tcgcccccct cgcggggcgg
     2281 ctttcccgtg cccgagatcg actcgctata ctggctcgga gggaacacgt caagcgtggg
     2341 cgggcgccca cgcttgttgt ttggaggcga tggacctgga gaccacgatc ggcccggtcg
     2401 tcgagggcat gggcctcgag ctcgtcgagg cgaccttccg gaaggagggt ggccgccgcg
     2461 tgctccgcgt caccgtggac cgtgacggcg gcatagacct cgacacgatc gcggagacca
     2521 gcgagaaggt ctcgcggcgg ctcgacgcgc tcgacttcgg cggggatgcg gcctacacgc
     2581 tcgaggtctc ctcgccgggc gtcgagcggc cgctgaggaa gccggaggac ttcgccaagc
     2641 gggtgggcga gagggtgaag gtcaagacgt acgcgccgat cgacggcgag cgctcgcaca
     2701 ggggcgagat cgtggcggcc gacgaggagg cggtcacgat tcgcgtggag gacgccgagc
     2761 ggcggatggc atgggacgac atcgcctccg ccagaacgat cttcgagtgg gggccgtcat
     2821 gaacctggag ttcctcgagg cgctgcggga gctcgagcgc gagcggggca tctcgttcga
     2881 ggtgctgctg cacggcatgg aggaggccct ggcctccgcc tggaagt
//
LOCUS       DASUTW010000264         3845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1380825, whole genome shotgun sequence.
ACCESSION   DASUTW010000264 DASUTW010000000
VERSION     DASUTW010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380825"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..1286)
                     /locus_tag="VF058_08895"
     CDS             complement(174..1286)
                     /locus_tag="VF058_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208457.1"
                     /translation="MEDAGQPATRAPEDRPRFDWDEGPARPRPPRPRRLAARIFAFIG
                     AVVLWLSIAFLLVLVTGEILTKDYYLSEGLWEAAARRFDAASAVVIGVFLIVSMGLIA
                     KRVGYRFRDGLMVFIPIYSYVFMVKMLWRWSSLPERPWDDRGRDRIDGPDPGEPTEVE
                     SRASRRPIMIGGLGIMALAVWIVLFSIQPWAPEQGGWRTTEANGVSLSLPSSFAVFTD
                     PDEIAQVVMEGTDPSAREVAEIIQQFPDFFVLLASEFSEEGTETGSLVLVLRLPSFGV
                     PFDEMISSYEEDFEGYELASPVSRQAVDVGVSGLRAVRFDYDHRLPGFPGEVHSADFV
                     VDGGMQVWLLQTINPADRWEEHTSRLDRIVASLAFT"
     gene            complement(1320..1955)
                     /locus_tag="VF058_08900"
     CDS             complement(1320..1955)
                     /locus_tag="VF058_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208458.1"
                     /translation="MTLRWGAPSAGATADTYGVYRDGTLLENLEAGSSTFVDTTVLPE
                     QEYEYLVRTLSKGVSSEDATVDVETPAAPLALARVAGTYDIRARTTSRSGYDDYPDQQ
                     TRAWSLKPRCKEGTCDVVWRDLAGKDQPIRLSRKGPSYRGSGSSRTFALCQGVPATSS
                     VDVALTVEKAKVVKGRWIATRLTGTLHHAESAQLGCGTSSIDLAVRAIRVG"
     gene            complement(2273..2539)
                     /locus_tag="VF058_08905"
     CDS             complement(2273..2539)
                     /locus_tag="VF058_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208459.1"
                     /translation="MDQTLFMVEGYVAGITREQVVSGVARLKAAATEMGREGIEILHV
                     GSTFVPSEGSIFSLVRGASRSDVEELSRRGRFPFHRITEAEFVE"
     gene            2698..3636
                     /locus_tag="VF058_08910"
     CDS             2698..3636
                     /locus_tag="VF058_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208460.1"
                     /translation="MIVGSRHAQSFWRRSLVLVAALAVALTNALPAEAVAPQTVVATN
                     KAEWKAAATDQWLAWTVWTGRADIVYAKPVGGSRFRVNRKGSNGVLGAIDGTTLVYQE
                     FTLGRRGRSSIWFMDLETRTKTKAPGAVNSRQWEYWPSLSGDWLLFARHLNTGGRRLY
                     LFNRTTQERRLVASMSRGRRVLLTGQVRGEYVSFEKTTWDRRGRATSCNVYRYDIASS
                     ATTLIPNPEKKCQWGPSVNDEGTVYFARGGFACGRGVSLRSHPIGGPTQVIWQLPKKN
                     SLTTTYAWDREDDATNVYFGTYKCGRWADIKMIPGL"
     gene            complement(3614..>3845)
                     /locus_tag="VF058_08915"
     CDS             complement(3614..>3845)
                     /locus_tag="VF058_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6208461.1"
                     /translation="APLGLTPSEQRVADLVASGMTNKEVAAALFISPKTVDSNLSRIY
                     RKLGVRSRTELAAKFPPESAPDAAEITDPESS"
BASE COUNT          668 a         1288 c         1305 g          584 t
ORIGIN      
        1 gaccaagagc gatacgagga cgtctggcag gacttgcagg gcgagctggc cgccctctcg
       61 tcacgcgggg agcgccgggt ggccgagggc gcgagccaca tgatccacca cgaccgcccc
      121 gacctcgtgg tcgaggcgat cggcgacgtg gtcgacgcag aacggcgatc gccctaggtg
      181 aaggcgagcg aagcgacgat ccgatcgagc cgagacgtat gctcctccca tcggtcggcc
      241 gggttgatgg tctgcaggag ccagacctgc atgccgccgt ccaccacgaa gtcggccgag
      301 tgaacctcgc cggggaatcc gggcaggcgg tggtcgtagt cgaaccggac ggcacggaga
      361 ccggagaccc ccacgtccac cgcctgccgt gagacggggc ttgccaattc gtagccctcg
      421 aaatcctcct cgtatgagga gatcatctcg tcgaagggca ccccgaagct ggggagccgc
      481 aggacgagca cgaggcttcc ggtctccgtc ccctcttcgg agaattcgga ggcgaggaga
      541 acgaagaagt ctgggaactg ctgaatgatc tccgcgacct cccgggccga ggggtcggtg
      601 ccttccatca cgacctgagc gatctcgtcg gggtccgtga agacggcgaa gctcgatggg
      661 agggagagag acaccccgtt cgcttccgtc gtgcgccatc ccccctgttc gggtgcccac
      721 ggctggatcg agaagagaac gatccacacg gcgagggcca tgatcccgag ccccccgatc
      781 atgatcggac gccgagatgc ccgggactcg acttccgtcg gctcccccgg atcgggtccg
      841 tcgatccggt cccggcctcg gtcgtcccag ggacgttccg ggagggagga ccagcgccac
      901 agcatcttca ccatgaagac gtaggagtag atcgggatga agaccatgag cccgtcgcgg
      961 aacctgtagc caacgcgctt ggcgatgagc cccatgctca cgatcaggaa gactccgatc
     1021 acgacggccg aggccgcgtc gaatctccgg gcggcagcct cccacaggcc ctccgatagg
     1081 tagtagtcct tcgtaaggat ctccccagtc accaggacga gaaggaacgc gatcgacagc
     1141 cacagcacca cggcgccgat gaaggcgaag atgcgcgcgg cgagacgtcg agggcgaggc
     1201 ggacggggcc tcgcggggcc ttcatcccag tcgaagcggg gccggtcctc cggtgcccgc
     1261 gtggcgggct gacctgcgtc ttccatcgaa cctggcggat gcggatccat cgccgcctcc
     1321 tatccgactc ggatcgcccg gaccgccaag tcgatgctgc tcgttccgca ccccagctga
     1381 gcggactccg cgtgatgaag cgttccggtc agccgggtcg cgatccagcg gcccttgacg
     1441 accttcgcct tctcgaccgt gagggcgacg tccaccgacg acgtggccgg caccccttga
     1501 catagtgcaa acgtccggga ggagcccgat cctcggtacg agggaccctt gcggctgagc
     1561 ctgatgggct ggtccttgcc ggccagatcc ctccagacga cgtcacacgt cccctccttg
     1621 catcgaggct tgagcgacca ggcgcgggtc tgctggtcgg ggtagtcgtc gtagcccgac
     1681 ctcgaggtcg tccgcgcgcg gatgtcgtag gtcccggcga cccgggccag ggcgagcgga
     1741 gcggcgggag tctcgacgtc gaccgtcgcg tcctcggatg agaccccctt cgagagcgtc
     1801 cgcaccaagt actcgtactc ctgctccggg agaacggtcg tgtcgacgaa cgtcgaggaa
     1861 cccgcctcca ggttctccag cagcgtcccg tccctgtaga cgccgtacgt gtcggcggtc
     1921 gctccggcgc tcggcgcacc ccacctcagc gtcacctcga agggcctggc ttgtgcctcg
     1981 agatcggcca cggccccggg gaccgaaggg gatggtgatg gcgagggcgg aggcgaggcg
     2041 gcggctggcg acgttgagtc tcctcgtccg gcgatcgtga tgaggagagc ggtcaccgcc
     2101 aggatcacga cccccgcgac cgcggtcagg atggtctggc gccggcgacg ccgctcgggc
     2161 ggcggcggcc cgggcgtctc acccggatga tcgaggctgc ccgtggaccc tcccccgtcg
     2221 ggggcactct ctgtgggcgg aacgataatg ttcatgccac cccacccctc cctcactcca
     2281 cgaactcggc ctcggtgatg cggtggaagg ggaaacgacc acgcctgctc agctcctcga
     2341 catcgctccg ggaggcgccc cgaacgagcg agaagatcga gccctctgac gggacgaacg
     2401 tggagccgac atggaggatc tcgatgccct cccgccccat ctcggtcgcc gccgccttca
     2461 gcctggcgac gcctgacacg acctgctccc gggtgatgcc ggccacgtac ccctccacca
     2521 tgaagagcgt ctgatccatc accgcgaggg taggtgggca agcgatccga ggaatcggga
     2581 ggatccccct atctgtgggg tgccgaagac ggaaagcaca gatcggggga tcctcccgat
     2641 tcctcgcgcc ttcccctctc ctacgctcac agcagtccaa agccgaggag gccgaccatg
     2701 atcgtcggat cgagacacgc tcagtcgttc tggcggaggt ccctcgtcct cgtcgccgcc
     2761 ctcgcggtgg ccctgaccaa cgctcttcca gcggaggcgg tcgctcctca gaccgtcgtc
     2821 gccaccaaca aagccgagtg gaaagctgcg gcgacggacc agtggctcgc ctggacggtg
     2881 tggaccggga gggcggacat cgtgtacgcg aagccggtcg gcggctccag gttccgggtc
     2941 aaccgcaagg gatccaacgg ggtgctggga gcgatcgacg gcaccacgct cgtctaccag
     3001 gagttcaccc tcggacgtcg cggccgctcc agcatctggt tcatggacct cgagacgagg
     3061 acgaaaacga aggctccggg ggccgtgaac tccaggcagt gggagtactg gccgagcctc
     3121 tcgggagact ggctcctctt cgcgcgccac ctcaacaccg gcgggcgacg cctctacctg
     3181 ttcaaccgca ccacgcagga gcgcaggctc gtcgcttcga tgtcgcgagg aaggagggtg
     3241 ctgctgacgg gacaggtccg cggcgagtac gtctccttcg agaagaccac ctgggaccgt
     3301 cggggcagag cgacctcgtg caacgtctac cgatacgaca tcgcgtcgag cgcgacgacc
     3361 ctgatcccga acccggagaa gaagtgccag tggggcccgt cggtgaacga cgagggcacc
     3421 gtgtacttcg ctcgtggtgg gttcgcctgc ggccgggggg tctccctgcg gagccacccg
     3481 atcggtgggc ccacccaggt catctggcag ctgcccaaga agaactcgct gacgacgacc
     3541 tacgcgtggg accgtgagga cgacgcgacg aacgtgtact tcgggaccta caagtgcggg
     3601 cggtgggccg acatcaagat gattccgggt ctgtgatctc ggcagcgtcg ggcgcggact
     3661 cgggagggaa cttcgcggcg agctcggttc gagacctgac gcccagcttc cggtagatcc
     3721 gggagagatt ggagtcgacc gtcttggggc tgatgaacag cgccgcggcc acctccttgt
     3781 tggtcatgcc gctggccacg aggtcggcga cgcgctgctc gctcggcgtg aggcccaggg
     3841 gcgcg
//
LOCUS       DASUTW010000265         2879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1380875, whole genome shotgun sequence.
ACCESSION   DASUTW010000265 DASUTW010000000
VERSION     DASUTW010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380875"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VF058_08920"
     CDS             <1..503
                     /locus_tag="VF058_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX6208462.1"
                     /translation="MSGARVEVDLRPQEAGPDGTEAAELLFAGGPGQAPQPLRKVASG
                     GELSRTMLACRTILADLDEVPTLVFDEVDAGIGGRAGVAVGRRLARLAGSRQVLVVTH
                     LPQIACFADRHFHVAKEDGRTTVRALEGEERVAELSRMLSGMPTEEAATHAEQLLAEA
                     DKEKRG"
     gene            500..1207
                     /locus_tag="VF058_08925"
     CDS             500..1207
                     /locus_tag="VF058_08925"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX6208463.1"
                     /translation="MRARWVAAAALLALGVWIPALVGVAGGARSTPPRQPSIEGGSAP
                     PATPVFAEALGIRLHLPASSPRLVAFHEASMEGALPMLPHGRCRPCRHPRFRPGPAGD
                     LRYAVMDPRGRGSPPTSAVDVVVSGEARVLSPVSGRVIEARRYRLYGRHPDVRVAIAP
                     DGAPGIEVVLLHLRSGRLAPGDRVVAGVTPLGRARSFSFRSQVDRYTAGRLPHVHVEV
                     VDARARNQAQSRRGPGG"
     gene            1200..2306
                     /gene="steA"
                     /locus_tag="VF058_08930"
     CDS             1200..2306
                     /gene="steA"
                     /locus_tag="VF058_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HEX6208464.1"
                     /translation="MDRRTKRLLTRVQRGEIAVIDHEDLDRVAAEGLVAREVAAVVNA
                     SPSITGRYPNLGPLLLAEGGIPLVDGVGPLLMEKVREGMQVRVEGDRVYADDALVGVG
                     VRQTPETVRRAMEEAREGLSEQLQAFARNTLEYMQRERDLLFGGEGMPELHEDLAERH
                     VLVVVRGPNYREDLAALRPYIREVKPLLMGVDGGADALLEAGYRPDIVIGDMDSVSEE
                     ALRSGAQIVLHAYPDGTAPGRARLEALGLPFSVLRSSGMSEDVAYLLAHEKGAELIVS
                     VGSHGNLREFLDKGRKGMSSTFLVRLRVGEILVDAKGVSRMYRSRIRGRDVAMLVGAA
                     LFSMVAVVAVSQPLRLFFKQLWELIRQALFSIGF"
     gene            2349..>2879
                     /locus_tag="VF058_08935"
     CDS             2349..>2879
                     /locus_tag="VF058_08935"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HEX6208465.1"
                     /translation="MISFRFHLVSLVAVFLALGLGVLTGTTVLNRGIVAQLERTTDRL
                     SEQSGQLREEVARLEARLGEWGDFGAEIQAYVVTGRLAEEEIVLVTQEGTDPAAVDAA
                     RQALELAGASVRLDLTVRLRMALTDTGDRDTIARVLEADAEDDPGALRDRAAETIADQ
                     VAFGSTSGNVIDRLADE"
BASE COUNT          363 a         1011 c         1103 g          402 t
ORIGIN      
        1 ggatgtcggg ggcccgcgtc gaggtggacc tccgccccca ggaggcgggg cccgacggca
       61 ccgaggccgc cgagctgctc ttcgccggcg ggccggggca ggccccgcag ccgctgcgca
      121 aggtggcctc ggggggcgag ctctcccgga cgatgctcgc ctgccgcacg atcctcgccg
      181 acctcgacga ggtccccacc ctcgtcttcg atgaggtcga cgccggcatc gggggacggg
      241 cgggggtggc cgtgggccga cgcctggcca ggctggccgg ctctcggcag gtcctcgtcg
      301 tgacccacct ccctcagatc gcgtgcttcg ccgaccgcca cttccacgtc gcgaaggagg
      361 acggcaggac gacggtccgc gccctggagg gggaggagcg ggtggccgag ctgtcgcgga
      421 tgctctcggg gatgccgacc gaggaggcgg ccacgcacgc cgagcagctc ctggccgagg
      481 cggataagga gaagcggggg tgagggcccg gtgggtcgcc gccgcggccc tgctcgccct
      541 cggcgtgtgg atcccggccc tggtcggggt ggcgggcggc gcgagatcca ctccgccccg
      601 ccagccgtcg atcgagggcg gatccgcgcc gcccgccacc cccgtgttcg ccgaggccct
      661 ggggatccgg ctccacctac cggcctcctc gccgcggctc gtggccttcc acgaggcctc
      721 gatggagggc gctctcccga tgcttccgca cggacggtgc cggccttgtc gccatccccg
      781 cttccggccc gggcccgcgg gcgacctgcg atacgcggtg atggaccccc gggggagagg
      841 gtcgccgccg acgtccgcgg tggacgtcgt cgtgagcggc gaggcccggg tcctgtcgcc
      901 cgtgagcggc cgcgtgatcg aggcccgtcg ctaccgactc tacgggcgtc accccgacgt
      961 gcgggtggcg atcgcccccg acggggcgcc ggggatcgag gtggtcctgt tgcacctccg
     1021 cagcggtagg cttgcaccag gggatcgcgt cgtggcgggt gtcacgccgc tgggacgggc
     1081 caggtcgttc tcgttccgct cccaggtcga tcggtacacg gcgggccggc tgccgcacgt
     1141 gcacgtggag gtggtggatg ctcgggctcg gaaccaggcg cagagccggc gcggcccggg
     1201 tggataggcg tacgaagcgc ctcctcaccc gggtgcagcg cggcgagatc gccgtgatcg
     1261 accacgagga cctcgaccgc gtcgccgcgg agggcctcgt ggccagggag gtcgccgccg
     1321 tggtgaacgc cagcccctcg atcaccggcc gctacccgaa cctggggccg ctcctcctgg
     1381 ccgagggcgg catccccctc gtggacgggg tggggccgct cctcatggag aaggtccgcg
     1441 aggggatgca ggtccgggtg gagggcgacc gcgtctacgc cgacgacgcc ttggtgggcg
     1501 tgggcgtccg ccagaccccc gagaccgttc gccgggcgat ggaggaggcg cgggagggcc
     1561 tctccgagca gctccaggcc ttcgcccgca acacgctgga gtacatgcag cgggagcgcg
     1621 acctcctctt cgggggggag gggatgccgg agctccacga ggacctcgcc gagcggcacg
     1681 tcctggtcgt ggtccggggc ccgaactacc gggaggatct cgcagccctt cgtccctaca
     1741 tccgcgaggt gaagccgctc ctgatggggg tggacggcgg cgccgacgcc ctgctcgagg
     1801 ccggctaccg ccccgacatc gtgatcgggg acatggacag cgtctccgag gaagccctca
     1861 ggtcgggcgc ccagatcgtg ctgcacgcct acccggacgg caccgccccc ggccgggccc
     1921 gcctggaagc gctcgggctg ccgttctcgg tgctgcggtc ctcagggatg agcgaggacg
     1981 tggcctacct cctggcccac gagaagggcg cggagctcat cgtctccgtg gggagccacg
     2041 ggaacctccg ggagttcctg gacaagggga ggaagggcat gtcgagcacg ttcctggtgc
     2101 ggctccgcgt gggggagatc ctggtggacg ccaagggcgt ctcccggatg taccggagcc
     2161 gcatccgggg acgggacgtc gccatgctcg tgggagccgc gctcttctcg atggtcgccg
     2221 tggtggccgt gtcccagccc ctgcgcctct tcttcaagca gctgtgggag ctgatccgcc
     2281 aggcgctctt ctcgatcggc ttctgatcgg ggctacgata ggcggcgcat gatccgggcc
     2341 gggcgccgat gatctcgttc cgcttccacc tcgtgtcgct ggtggcggtg ttcctcgccc
     2401 tggggctggg cgtgctgacg ggcaccaccg tcctcaaccg gggcatcgtg gcgcagctcg
     2461 agcggaccac cgatcgactc tccgagcagt cgggccagct tcgcgaggag gtcgcccggc
     2521 tcgaggcgag gctgggcgag tggggcgact tcggcgcgga gatccaggcg tacgtcgtga
     2581 cgggcaggct ggcggaggaa gagatcgtcc tggtgaccca ggagggcacc gaccccgcgg
     2641 cggtggacgc cgcccggcag gccctcgagc tcgccggcgc cagcgtccgc ctcgatctca
     2701 cggtccgcct gcggatggcc ctcaccgaca ccggggaccg ggacaccatc gctcgggttc
     2761 tcgaggccga cgcggaggac gacccgggcg cgctccggga ccgcgcggcc gagacgatcg
     2821 ccgatcaggt ggccttcgga tccaccagcg ggaacgtgat cgaccgcctg gccgacgag
//
LOCUS       DASUTW010000266         5056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_475189, whole genome shotgun sequence.
ACCESSION   DASUTW010000266 DASUTW010000000
VERSION     DASUTW010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475189"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VF058_08940"
     CDS             <1..373
                     /locus_tag="VF058_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="HEX6208466.1"
                     /translation="HDPVVIGMCETTAPVVDVTVSPDVLWPPDHRYRDVTASVSVTDA
                     DPNPTVELVSVTSNEPDDGEDDGSTVNDIVIVDDFNFRLRAERSGTGTGREYTITYLV
                     TDACGNSTTASAVVSVPRSRG"
     gene            462..1652
                     /locus_tag="VF058_08945"
     CDS             462..1652
                     /locus_tag="VF058_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208467.1"
                     /translation="MPLVFIHGVGTREGPGFEDAVDARDAMFRRFMLARTVTDPSAVT
                     ITNPYWGRHGARLAWDHASLPGSPVEALGPEDEDLAVLSAPILEDRPPSGAPLLATAR
                     RSLEEAVDLLWAAAAEISAGASDELARMAARAVDYARHEPDLGWLDEVQTDEEFLEEL
                     ERRVEAWTGDEAAPSGEPEPAYEALGAAESWARIGEAADRIKGFVGRLAGRAVVEVAR
                     APAHRSTALFLGDILVYLRQRGMAAAPGPIVVEVADALDAARQAVTAEDPRLIVVAHS
                     MGGNIAYDLLTHFRPDLEVDVLVTVGSQVAFFEELKLFAESDEAVPKSAGDRAPKPPN
                     VSRWINIFDRNDVLGFVAEGVFSEVEDYEYSTGKGVLFAHTTYFVRPSFHDRLGERVA
                     GPAA"
     gene            1649..2788
                     /locus_tag="VF058_08950"
     CDS             1649..2788
                     /locus_tag="VF058_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX6208468.1"
                     /translation="MTTLFDDEADGPRTHLFTMGVGTYRHLAGGDEPLDHLRGVGLGQ
                     LSSPPLSARAVTDWFIAEMDNPDAPLGSVELLLSPSQAYDLPDGRSVEVEAGTIDNLR
                     RAFDAWFARCDRHEGNVAILSFCGHGLMREVVALLAEDFGRSAQRPFEASVDFTMTFE
                     GMATCRARRQLYVVDACRQVMRSTLALQRIDATALAIGSFQREYERDAPILYASAEDD
                     RAYARAGHPTRLTEALLRGLRGLGSEIVGGGWVVTSRRLLEATSDLVVRENLPDAPMQ
                     RPRLGGESAGLAVPLHRLPGPPKVPVTIECLPGEAMEVAELSLVPVRPGPSYGRGPSP
                     ERWTLEVEPGDYDAAARFSGGDYPECRKPFVAMPPPPPPFPLEVG"
     gene            2788..4983
                     /locus_tag="VF058_08955"
     CDS             2788..4983
                     /locus_tag="VF058_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HEX6208469.1"
                     /translation="MGLLRLRVDSRSDIASGAPLVASVLTEDLETVTEVTVPLGGMRD
                     VQVGPGSYVIRAVAPTGSALTQRASVGPEASVEVELGHVASPHEWLELHALLGEVSAS
                     GTSDELGSGVWLRVWRWEADGTWTIEPVTWTTTWESEHVLARAAGLQPAQRVLQVGGE
                     SIPWRLLALPSGERIEVLLRRADGTPELDAGVEASVVTDRTSGEVLHGYLARGRTEAA
                     GIVAEEMLQGSRPDGLESPTSAAVLGYYLVRRGQEERLAGLALVERFPWLPDAWILEG
                     WRALRGGDEQAGRAHLLRAASMGAPLHSEGMRLLSTGLRLLEAASPADADLRSATESL
                     SAVAAATNWGEPLTTYYGLDPTTPTLAPPTGIPDGPHTRIGPDRGGTPPPRPRWGRWV
                     LGSAVVAAVIVGVVWWATRGGGGLAIDPSSLDYGERPIEAAGASEAVTLSNDTGAEVL
                     VRNAKIRGPDADDFDLTTDTCSGVALPDGRSCSLVVSFTPTEEGAREARLVIRIEGEG
                     GPIRVPLDGTGVSGEPEEEEGSPVIVVDPPTITFPADVREGAVHELTITNDGDAVLEV
                     AASVDPADEGIALDPDSVSACAEIQPGDSCTMSVLFVPFRGSRGEANVVIENNAIEQP
                     LQVRVSGTPRAGPTPAEVGTTDWDGSQANVLVRNTGTRAFTPAFDFENQRFSIASHTC
                     GELVIGGECAVNISFDAEGAEDLVGALVIEDGSGEGPVVVPLEYPTPVD"
BASE COUNT          692 a         1748 c         1892 g          724 t
ORIGIN      
        1 ccacgacccc gtcgtgatcg ggatgtgcga gacgacggcc ccggtcgtgg acgtgacggt
       61 ctcgccggac gtgctgtggc cgcccgatca caggtaccgg gacgtcacgg cctccgtctc
      121 ggtcaccgac gcggacccga acccgacggt cgagctcgtc tcggtgacct cgaacgagcc
      181 ggacgacggc gaggacgacg ggagcaccgt caacgacatc gtgatcgtgg acgacttcaa
      241 cttccggctc agggccgagc gctcgggcac gggcacgggg cgggagtaca cgatcaccta
      301 cctcgtgacg gacgcctgcg ggaacagcac gacggcgtcc gcggtggtct ccgttcctcg
      361 aagccggggc tgagcggccg tagcgcgttc ggcggaggcc gaacggccga tggtgtccgg
      421 tgggcggccg ctctactgtt cccggactcg aggaggttgt catgcctctg gtgttcatcc
      481 acggcgtggg gacccgggag ggcccggggt tcgaggacgc ggtcgacgcc agggacgcca
      541 tgttccggcg gttcatgctc gcccgaacgg tgaccgatcc ctctgccgtt acgatcacga
      601 acccgtactg gggacgccac ggcgcccgtc tcgcctggga tcatgcatcc ctccctgggt
      661 cccccgtcga ggcgctggga cccgaggacg aagatctcgc cgtgctgagc gccccgatcc
      721 ttgaggatcg cccgccgagc ggcgcccccc tgctggccac ggctcggcgg tcgctcgaag
      781 aggcggtgga cctgctgtgg gcggccgcgg ccgagatctc cgccggcgcc tcagacgagc
      841 tcgcacggat ggcggcccgg gccgtggact acgccaggca cgagccggat ctcgggtggc
      901 tggacgaggt gcagacggac gaggagttct tggaggagct cgagcgccga gtggaggcgt
      961 ggaccgggga cgaggcggcc ccctcgggtg agcccgagcc cgcctacgag gcgctgggcg
     1021 ccgccgagag ctgggcccgg atcggagagg ccgccgaccg gatcaagggc ttcgtcgggc
     1081 ggctcgcggg tcgggccgtc gtggaggtgg ccagggctcc cgcccaccgc agcaccgcgc
     1141 tcttcctcgg cgacatcctc gtgtacctcc gtcagcgggg gatggccgcc gcgccggggc
     1201 ccatcgtcgt ggaggtggcg gacgcgctgg acgccgcgcg gcaggccgtg accgccgagg
     1261 atccgcgcct gatcgtggtc gcccacagta tgggagggaa catcgcgtac gacctcctca
     1321 cccacttccg cccggacctg gaggtggacg tgctggtgac ggtgggatcg caggtcgcgt
     1381 tcttcgaaga gctgaagctg ttcgccgaga gcgacgaggc cgtcccgaag agcgcgggcg
     1441 atcgcgcgcc gaagcccccg aacgtctcgc gctggatcaa catcttcgat cgcaacgacg
     1501 tgctggggtt cgtggcggag ggggtcttca gcgaggtcga ggactacgag tactcgacgg
     1561 ggaagggggt cctcttcgcg cacaccacgt acttcgtgcg gcccagcttc cacgaccgcc
     1621 tcggcgagcg cgtggcgggg ccggcggcat gacgacgctc ttcgacgacg aggccgacgg
     1681 cccgcgaacc catctgttca cgatgggagt ggggacgtac cggcacctgg caggtggtga
     1741 cgagcccctc gaccatctgc ggggcgtggg cctgggccag ctctcctcgc cgcccctgtc
     1801 cgcccgcgcg gtgacggact ggttcatcgc agagatggac aacccggatg ctcctctggg
     1861 aagcgtggag ctgctgctgt cgccgtccca ggcctacgac ctcccggacg gccgctcggt
     1921 cgaggtggag gcgggcacga tcgacaacct ccgacgggcc ttcgacgcgt ggttcgctcg
     1981 atgcgatcgt cacgagggca acgtggcgat cctctccttc tgcggccatg gcctcatgcg
     2041 agaggtcgtg gcgctgctcg cggaggactt cggacggagc gcccagcgtc ccttcgaggc
     2101 ttcggtcgac ttcacgatga ccttcgaagg gatggccacg tgccgagccc gacgacagct
     2161 ctacgtggtc gatgcgtgcc ggcaggtgat gcgctccacc ctggctctcc agcggatcga
     2221 cgccacggcc ctcgcgatcg gcagcttcca gcgcgagtac gagcgagacg ccccgatcct
     2281 ctacgcctcc gctgaggacg atcgcgcata cgcgagggcg gggcatccca cccgtctgac
     2341 ggaagcgctg ctcaggggcc tccgcggcct ggggagcgag atcgtcggcg gcggatgggt
     2401 cgtgacgtcc cggaggctgc tcgaggcgac ctcggacctc gtcgtccggg agaacctgcc
     2461 cgacgccccg atgcagcgcc cccgcctcgg cggtgagtcc gccggcctcg ccgtgccgct
     2521 gcatcgcctc cccggcccgc ccaaggtgcc ggtcacgatc gagtgccttc ccggcgaggc
     2581 gatggaggtg gccgagctct ccctcgtgcc ggtgcggccc gggccctcgt acggccgcgg
     2641 gccgtcgccc gagcggtgga cgcttgaggt ggagccggga gactatgacg ccgcagctcg
     2701 cttctccggc ggcgactacc cggagtgccg gaagccgttc gtggccatgc cgccaccacc
     2761 accacccttc ccattggagg tgggctgatg gggctcctgc gcctccgggt cgacagccgg
     2821 tccgacatcg cctccggcgc tccgctcgtg gcctccgtcc tgaccgagga cctggagacg
     2881 gtcacggagg tcacggtgcc gctcggcggg atgcgcgacg tccaggtggg gccgggatcc
     2941 tacgtgatca gggcggtcgc tccgaccggc tccgcgctga cccagcgggc gtcggtgggc
     3001 ccggaggcgt cggtcgaggt cgagctgggg cacgtcgcct ctccccacga gtggctcgag
     3061 ctgcacgcgc tgctcggcga ggtgtccgcc tccgggacgt cggacgagct cgggtcgggg
     3121 gtctggctgc gggtctggcg ttgggaggcc gacgggacgt ggacgatcga gccggtcacg
     3181 tggacgacga cctgggagag cgagcacgtc ctggcccggg ccgccgggct ccagcccgcc
     3241 cagcgggtcc tgcaggtcgg gggggagtcg atcccatgga ggctcctggc gctgccttcc
     3301 ggcgagcgga tcgaggtgct gctgcggcgg gccgacggca cgccggagct cgacgccggc
     3361 gtggaggcct cggtcgtcac cgatcggacg agcggcgagg tgctccacgg ctacctcgcc
     3421 cgcggacgaa ccgaggccgc gggcatcgtg gccgaggaga tgcttcaggg atcccggccg
     3481 gacggactcg agagccccac gtccgccgcc gttctcggct actacctggt gcggaggggg
     3541 caggaggagc ggctggccgg gctggcgctg gtcgagcgat tcccctggct ccccgacgcc
     3601 tggatcctcg aagggtggcg cgcgctccga gggggggacg agcaggccgg tcgggctcac
     3661 ctgctccgcg cggcctcgat gggtgcgccc ctccactcgg agggcatgag gctcctctcc
     3721 acgggcctcc ggctcctgga ggcggcatcc cccgcggacg ccgacctccg gagcgcgacc
     3781 gagagcctgt ccgcggtcgc cgcggccacg aactgggggg agcccctcac gacgtactac
     3841 gggctcgacc ccacgacccc cacgctcgct ccgcccaccg gcatcccgga cgggccgcac
     3901 acccggatcg gcccggaccg aggcggaact ccgcctccca ggccccgttg gggccgctgg
     3961 gtcctcggct cggccgtggt cgcggcggtg atcgtcgggg tcgtctggtg ggcgacgagg
     4021 ggcggcggag gcctcgccat cgacccgtcc tcgctcgact acggcgagcg tccgatcgag
     4081 gccgcggggg cctccgaggc ggtcaccctc tcgaacgaca ccggggcgga ggtgctggtc
     4141 cgaaacgcga agatccgcgg gccggacgcc gacgacttcg acctgacgac cgacacgtgc
     4201 tccggcgttg cccttcctga tgggcggtcg tgctccctgg tggtctcctt caccccgacg
     4261 gaggaggggg cccgggaggc gaggctcgtg atccgcatcg aaggcgaggg agggccgatc
     4321 cgggtcccgc tcgacgggac cggcgtgtcg ggggagccgg aggaggagga gggaagcccg
     4381 gtgatcgtcg tggatccccc gaccatcacc tttcccgccg acgtccggga gggggccgtc
     4441 cacgagctga cgatcacgaa cgacggagat gccgtcctcg aggtcgcggc gagcgtggac
     4501 ccggcggatg agggcatcgc cctcgatccc gactcggtgt cggcatgcgc cgagatccag
     4561 cccggcgaca gctgcacgat gtccgtgctg ttcgtcccct tccgcggctc ccggggtgag
     4621 gccaacgtgg tcatcgagaa caacgccatc gagcagccgc tgcaggtccg ggtctcgggc
     4681 acgcctcggg ccggccccac ccccgcggag gtcgggacga cggactggga tgggtcgcag
     4741 gcgaacgtgc tcgtcaggaa cacgggcacg agggccttca cgccggcttt tgacttcgag
     4801 aaccaacggt tcagcattgc ttcgcacacc tgcggggagc tggtcatcgg cggggagtgc
     4861 gcggtcaaca tcagcttcga cgctgaggga gccgaggact tggtgggcgc gctggtgatc
     4921 gaggacgggt cgggggaggg gccggtcgtc gtcccgctcg agtacccgac gccggtcgac
     4981 tagcggaacc cgcgggcgac ctcggcctcg gcgacccggc ccaccccgat caggtatgcg
     5041 gcctcgcgga gcgtga
//
LOCUS       DASUTW010000267         3210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_88168, whole genome shotgun sequence.
ACCESSION   DASUTW010000267 DASUTW010000000
VERSION     DASUTW010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88168"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VF058_08960"
     CDS             complement(<1..394)
                     /locus_tag="VF058_08960"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX6208470.1"
                     /translation="MAENVAVVRERIEAACRRAGRDPSGVRLVAVTKTVDPSMVREAA
                     AAGIEDFGENYVQELEGKREAAPGARWHFLGRVQSNKAHRVAKLADMVQTVEPGRAWR
                     RLASAAEDEPIACLIQVDFTGERVGVEAA"
     gene            complement(400..1167)
                     /gene="pgeF"
                     /locus_tag="VF058_08965"
     CDS             complement(400..1167)
                     /gene="pgeF"
                     /locus_tag="VF058_08965"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX6208471.1"
                     /translation="MERRALTDGLNALVPEGMESHGFLAAFTERRGGVSEPPYDSLNL
                     GLHTGDRPDVVAENRRLVVRTLGLDRWAAGEQVHGGGVVRVDERWASRGWDGPPIDGA
                     DALAVARAGLPVAVLTADCLPIALASPLEGLLVAVHAGWRGLAAGIVDAALASFDRPG
                     EIHAAIGPAIGPCHYEVGEDVGRAVSEGSATGAVTDRRNGRLYLDLPATAVRVLRAGG
                     VRRIDRADLCTACEEDRFFSHRRDGITGRQALVAVRL"
     gene            complement(1273..2448)
                     /gene="ftsZ"
                     /locus_tag="VF058_08970"
     CDS             complement(1273..2448)
                     /gene="ftsZ"
                     /locus_tag="VF058_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018837710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX6208472.1"
                     /translation="MHAPQNYLAVIKVVGIGGGGVNAVNRMIEAGLKGVEFIAINTDA
                     QALLMSEADVKLDIGRETTRGLGSGSDPEIGRRAAEEHGDEIEEILKGADMVFITAGA
                     GGGTGTGGAPILARIARDLGALTIGVVTRPFSFEGKRRATQADMGIQELREHVDTLII
                     VPNDRLLQVSSEETPMLDAFRLADEILYQGVEGITSLIMTPGLINLDFADVKTVMTSA
                     GSALMGIGHGAGDDRAVEAAQNAVSSPMLEASIEGARGVLLSISGGSGLTLHEVNRAA
                     ETITRAAHPEANIIFGAVIDDSLGDECRVTVIAAGFDKLGTTTIVRPRTFEVEVGGED
                     LEEEEIPVPSILRESEDEDDVFQEAGEEEEEARPARVVISADDTDADEDLDIPDFLK"
     gene            complement(2578..>3210)
                     /locus_tag="VF058_08975"
     CDS             complement(2578..>3210)
                     /locus_tag="VF058_08975"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX6208473.1"
                     /translation="FEVRRVEVRGNGRVGPGQIRKLVQPAQGRNLWLVPLEDLARAVS
                     SHPWVARADIRRELPSTLVVEIEERIPIGWVADPGGGAAVAADGIVVQRRPEAAGLVS
                     LGAVDEIQRPGTPTVAPAASLRVAASLPARIRREVAAVSRRGEGIILRMRSGARILYG
                     RAEALGAKRATLAAVLSWIDENEVPAGIVDLRAPATPAIRPVSLRAGTRG"
BASE COUNT          438 a         1256 c         1059 g          457 t
ORIGIN      
        1 cggcggcctc gacgcccacc cgctcgcccg tgaaatccac ctggatcagg cacgcgatcg
       61 gctcgtcctc ggccgccgac gccagccggc gccacgcgcg gccgggctcc accgtctgca
      121 ccatgtctgc cagcttcgcc acccgatggg ccttgttgct ctgcaccctc cccaggaagt
      181 gccaccgtgc cccgggcgcg gcctcccgct tgccctccag ctcctgcacg tagttctcgc
      241 cgaagtcctc gatccccgcc gcggccgcct cccggaccat cgacggatcg accgtcttcg
      301 tcaccgccac caggcgcacc ccggacggat cccgccccgc gcgacggcag gccgcctcga
      361 tccgctcgcg gaccacggcc acgttctcgg ccaccccgct cacagccgca ccgccaccag
      421 cgcctgccgc ccggtgatcc catcccgacg gtgggagaag aaccgatcct cctcgcaggc
      481 agtgcacagg tcggctcgat cgatcctgcg gacgcctccc gcccggagca cgcgcacggc
      541 ggtcgcgggg aggtcgaggt acaggcggcc gttccggcgg tcggtgacgg cgcccgtcgc
      601 cgatccctcc gagacggcgc ggccgacgtc ctcgccgacc tcgtagtggc acgggccgat
      661 ggccgggccg atcgccgcgt ggatctctcc aggtcgatcg aagctcgcca gggcggcgtc
      721 gacgatcccg gccgccaggc cccgccatcc cgcgtggacc gcgaccagaa gcccctcgag
      781 aggagaggcc agcgcgatgg gaaggcagtc cgcggtgagc accgccaccg gcagcccggc
      841 ccgggcgacc gccagcgcgt cggccccgtc gatcggcggc ccgtcccagc cccgggatgc
      901 ccatcgctca tccacccgaa cgacaccgcc gccgtggacc tgctcgcccg ccgcccaccg
      961 gtcgagcccc agcgtccgga ccacgaggcg ccggttctcg gcgacgacgt cgggccggtc
     1021 ccccgtgtgc aggccgaggt tcaggctgtc gtacggtggc tcactcacgc cccctcgacg
     1081 ctcggtgaag gccgcgagga agccgtgaga ttccatgccc tccgggacga gggcgttgag
     1141 gccgtcggtc agcgcgcgtc gctccatcga gggtgggtgg gggcggggag ggccacaccg
     1201 tcaaggcggg ggaagggcgc ctcgttcggg ccctccccgc ccccacacct cgtggggggc
     1261 cgttccggcc ccctacttca ggaagtccgg gatgtcgagg tcctcgtcgg catcggtgtc
     1321 gtcggcgctg atcaccaccc gggccggacg ggcctcctcc tcctcctctc cggcttcctg
     1381 gaacacatcg tcctcgtcct cggactctcg caggatcgag gggacgggga tctcctcctc
     1441 ctcgaggtcc tccccgccga cctcgacctc gaaggtccgg gggcggacga tcgtcgtcgt
     1501 ccccagcttg tcgaagccag ccgcgatcac cgtgacgcgg cactcgtcac ccagcgagtc
     1561 atcgatcacc gcgccgaaga tgatgttcgc ctctgggtgg gcggccctcg tgatcgtctc
     1621 ggctgcgcgg ttgacctcgt ggagggtgag ccccgagccg cccgagatgg agagcagcac
     1681 gcccctggcc ccctcgatcg aggcctcgag catcggagac gagacggcgt tctgcgccgc
     1741 ctccaccgcg cggtcgtcgc cggccccgtg cccgatcccc atcagggccg agccggcgct
     1801 cgtcatcacg gtcttcacgt ccgcgaagtc gaggttgatg aggccggggg tcatgatgag
     1861 gctcgtgatg ccctccaccc cctggtagag gatctcgtcc gcgagccgga aggcgtcgag
     1921 catcggggtc tcctcgctcg agacctggag gaggcggtcg ttcgggacaa tgatcagcgt
     1981 gtcgacgtgc tcccgcagct cctggatccc catgtccgcc tgcgtcgccc gccgcttgcc
     2041 ctcgaaggag aagggccgcg tcaccacgcc gatcgtcagg gcgcccaggt cgcgcgcgat
     2101 ccgggccagg atcggggcgc cacccgtgcc ggtgccgccg cccgcgccgg ccgtgatgaa
     2161 caccatgtcg gcgcccttca ggatctcctc gatctcgtcc ccgtgctcct cggcggcccg
     2221 ccggccgatc tccgggtccg accccgagcc cagcccgcgg gtcgtctccc ggccgatgtc
     2281 cagcttcacg tcggcctcgg acatcaggag cgcctgagcg tcggtgttga tggcgatgaa
     2341 ctcgaccccc ttgaggccgg cctcgatcat ccgattcacg gcgttgacgc cgccgccgcc
     2401 gatcccgacg accttgatga ccgccaggta gttctgtgga gcgtgcatcg cgttcatccg
     2461 ctgcctcccc ggtagcctca gcgcccccac gctcgaagcc tcaacctaaa ctcaaggttg
     2521 aaagttatgt aaacctgccc tacagggcga ggaatctaca ccatcctgga gggggcgtca
     2581 accccgggtt ccggctcgga gggagacggg ccggatggcc ggggtcgcgg gtgcccggag
     2641 gtcgacgatg ccggcgggga cctcgttctc gtcgatccag ctcaggacgg ccgccagcgt
     2701 ggcccgcttc gcccccagag cctccgccct cccgtagagg atcctcgccc ccgacctcat
     2761 ccggaggatg atcccctcgc cgcggcggga gacggccgcc acctcgcggc ggatccgagc
     2821 ggggagggag gccgcaactc ggagagaggc cgccggggcg accgtcggcg tccccgggcg
     2881 ctggatctcg tccacggctc cgagcgagac gaggcccgcg gcctccggcc gccgctggac
     2941 cacgatcccg tccgcggcca ccgccgctcc cccgccaggg tccgcaaccc agccgatcgg
     3001 gatgcgctcc tcgatctcga ccacgagggt cgaggggagc tcgcgacgta tgtcggcgcg
     3061 ggccacccac ggatgggacg acacggcccg ggcgagatcc tccagcggca cgagccagag
     3121 gttcctccct tgggcgggct ggacgagttt ccggatctga cccggcccca ccctcccgtt
     3181 cccgcgcacc tccacgcgac ggacctcgaa
//
LOCUS       DASUTW010000268         4716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_735765, whole genome shotgun sequence.
ACCESSION   DASUTW010000268 DASUTW010000000
VERSION     DASUTW010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735765"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VF058_08980"
     CDS             complement(<1..203)
                     /locus_tag="VF058_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208474.1"
                     /translation="MPRSDERFLGPRDRARSRMLERRRRVLTVLLEGIGLTALIGSVP
                     PLREMWVLSGILVALLGAYVWMLL"
     gene            complement(443..1645)
                     /gene="glp"
                     /locus_tag="VF058_08985"
     CDS             complement(443..1645)
                     /gene="glp"
                     /locus_tag="VF058_08985"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX6208475.1"
                     /translation="MRSIQEARESILRAVEPLAPIELPLQESHGCVLAADVMAEYDLP
                     PFSSAEVTGYAARAADIHAATEDAPVPLRLSSGEPGRPAGGTVGWGEAVAVAAGAPVP
                     AGADCVVPLDRCRVEGGGVAVVAPVESGEFIRPAGRDVRAGETLVPAGRKLSSAEMAI
                     LAAAGRPAPPVYPRLRVGVMSIAQALAEPGRPAAFGQAREAASFAVVGALRDAGAVPY
                     RVGMVPPVEGELREAVVTNLARADSFVCTVGASDADLDPEALDVLDVEFEDVAMFPGM
                     TFGFGVVEGVSFFVLPASPVSAFVLFEMLVRPAILRMMGRRDVSRPEVRAVLEEDVSG
                     PSGVTMFVPARVEHRDGWRATPTGGGDQDSFAALVRANGLVMIPPGDTEPAAGDEVRV
                     RIFRPLER"
     gene            complement(1648..2523)
                     /gene="galU"
                     /locus_tag="VF058_08990"
     CDS             complement(1648..2523)
                     /gene="galU"
                     /locus_tag="VF058_08990"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX6208476.1"
                     /translation="MKVRKAVIPAAGLGTRFLPATKAQPKEMLPVVDKPAIQYVVEEA
                     VRAGIEDILIITGRGKRTIEDHFDRSVELEFYLEQAGKHEELKAVRHISEMASIHYIR
                     QKDPRGLGHAVAVAEPHVQGEPFAVLLGDDIVVADPLLERMIGVYERYGRSVIAVQEV
                     ARSDIHLYGSIKPEYVEDDLARVLDIVEKPAADEAPSTLGAIGRYVLTPEIFEALRET
                     SPGAGGEIQLTDAIKMLASEQAVYALVYEGERFDIGKKLDYLRATIELAVDRDDLGPG
                     LRAYLADLVQRKKLL"
     gene            complement(2560..4530)
                     /locus_tag="VF058_08995"
     CDS             complement(2560..4530)
                     /locus_tag="VF058_08995"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX6208477.1"
                     /translation="MTLRARLTLFFAGIVVVPLVAATLVLQILVTQEVRRRSDARLEA
                     ASATLSTLWQERLTVAERETGRAASVAAELLPGRGTALDERLEVERSAAGLDFLVVTN
                     PQGGIIASSMGSPLFARGVAPPTAFDIVGERSVPGLLRARAPISVSGNLGGVVGGWFV
                     DTEFLRELVGPADVDLILRSGMRVLAATEASLGDLPARGPFPVSVDDGRRVTVTQIRG
                     QTGEIVLVTPEEVAGVHGAVWGVAVLGLLIASMLGYLLAGVIGRPVYELAEGARRVAA
                     GDLHTRVMVGDRGDIGNLADAFNQMTENLETYVTRLEESRDELRRNLDRLGATLRSTH
                     DLDAMLSVIVDTAAATLHASTAALFLLTPSGKRLRLQAVLGYEPSAATEMRLGKGIAG
                     RAAEGRPVLVPSRLEEIPERSPAEPPATTAIAVPLVRGTSTIGVLALYGRTLPDPFTE
                     DDAATLASFAGQASVAIENVLLHQEAERLSLTDGLTGVWNRRFLQLQLQKEIDRAQRF
                     GRPLSLLLVDIDRFKATNDEHGHLVGDEVLVEVVRRIVDSTRSNIDVVARFGGEEFVA
                     LLPETPREGARVVAEKVRQAISLRPVQAEGVGAGVHVTVSVGVASYPEDGTTVDALLR
                     AADRAMYRAKEAGRDRVELAVSGESTARGSGG"
BASE COUNT          708 a         1761 c         1629 g          618 t
ORIGIN      
        1 agcagcatcc agacgtaggc gccgagcagg gccacgagga tcccgctgag gacccacatc
       61 tcgcgcagcg gagggacgga cccgatgaga gcagtcagcc cgatcccctc cagcagcacg
      121 gtgaggaccc ggcgtctccg ctccagcatc cgcgagcggg cccggtccct cggcccgagg
      181 aaccgctcgt ccgaacgagg catcagcacc ctgcgttgct gcgtgaacac cgagaagtct
      241 cctggcgtcg gccgcacgcc ccctctcgag cgccgcggca tgagcagtac ggcccagatg
      301 atgcccaaaa ccgcgagtag aagccaatcc aacgcccagc acctccgtcg gccgttcccc
      361 ggtcgagccg ccagatatgg gccttcggcg cacgctagca cccaagatgt agtggttcaa
      421 acatttccgg cgatctcgcc cctcagcgct ccaggggccg gaagatgcgc acgcggacct
      481 cgtctccggc cgccggctcc gtgtcgccgg gggggatcat gacgaggccg ttggcccgca
      541 ccagggccgc gaagctatcc tggtcgcccc cgccggtggg cgtcgccctc cacccgtccc
      601 ggtgctccac gcgggcgggg acgaacatcg tgacgccgga cgggcccgag acgtcctcct
      661 ccaggaccgc cctcacctcg gggcgggaga cgtcgcgtcg ccccatcatg cggaggatgg
      721 ccgggcggac gagcatctcg aagagcacga acgccgagac gggcgaggcg gggaggacga
      781 agaaggagac cccttccacg acgccgaagc cgaacgtcat gccggggaac atcgccacgt
      841 cttcgaactc gacgtcgagg acgtcgagcg cctcgggatc gaggtccgcg tcggacgctc
      901 cgacggtgca gacgaacgag tcggcgcggg ccaggttcgt cacgaccgcc tcgcggagct
      961 ccccctccac ggggggcacc atccccacgc gatacgggac cgcccccgcg tcccggaggg
     1021 cgcccacgac ggcgaacgag gcggcctccc tcgcctggcc gaaggcggcg gggcgcccgg
     1081 gctccgccag ggcctgggcg atgctcatca cgcccacccg cagccgtggg tacacgggcg
     1141 gagccgggcg ccccgccgcc gccaggatcg ccatctccgc ggacgacagc ttcctcccgg
     1201 cggggacgag ggtctccccc gcccgcacgt cgcgaccggc cggccggatg aactcgcccg
     1261 actcgacggg cgctacgacg gcgacccctc ccccctcgac acggcaccgg tcgagcggga
     1321 ccacgcagtc ggcgcccgcc gggaccggcg cgccggcggc caccgccacg gcctcgcccc
     1381 agccgaccgt tcctcccgcg ggccgaccgg gttccccgga cgacagcctg agcggcaccg
     1441 gggcgtcctc ggtcgcggcg tggatatcgg cggcccgagc cgcgtagccg gtcacctcgg
     1501 ccgaggagaa cggcggcaga tcgtattcgg ccatcacgtc tgcggcgagc acgcacccat
     1561 gggactcctg gagcggcagc tcaatcgggg cgagaggctc caccgcccgg aggatggact
     1621 cgcgcgcctc ctggatggag cgcacgatca gagcagcttc ttcctctgga ccaggtccgc
     1681 gaggtacgcg cggagccccg gccccaggtc gtcgcggtcg acggcaagct cgatcgtggc
     1741 gcgtaggtag tccagcttct tcccgatgtc gaaccgctca ccctcgtaga cgagggcgta
     1801 gacggcctgc tcgctggcga gcatcttgat cgcgtcggtg agctggatct ctccgcccgc
     1861 ccccggggag gtctcgcgga gcgcctcgaa gatctccggc gtcagcacat accgcccgat
     1921 cgccccgagc gtggacggcg cctcgtcggc cgcgggcttc tccacgatgt ccaggacccg
     1981 cgccaggtcg tcctcgacgt actcgggctt gatcgagccg tagaggtgga tgtcgctgcg
     2041 ggcaacctcc tgcaccgcga tcacgctgcg cccgtagcgc tcgtacacgc cgatcatccg
     2101 ctccagcagc gggtcggcca cgacgatgtc gtcgcccagc agcaccgcga acggctcgcc
     2161 ctggacgtgc ggctcggcca cggccacggc gtgccccagc cctcgcgggt ctttctgacg
     2221 gatgtagtgg atcgaggcca tctcggagat gtgccgcacc gccttcagct cctcgtgctt
     2281 ccccgcctgc tccaggtaga actcgagctc gacggagcgg tcgaagtggt cctcgatcgt
     2341 gcgcttccca cgacccgtga tgatgaggat gtcctcgatc cccgcccgga ccgcctcctc
     2401 gaccacgtac tggatcgccg gcttgtccac gaccggcagc atctccttgg gctgggcctt
     2461 cgtggccggc aggaacctcg tgccgagacc ggccgccggg atcacggcct tcctgacctt
     2521 cacgctccct ccagcgtcga cggacggtgc gccggaagtt tagccgcccg agcccctcgc
     2581 ggtcgactcg cccgagacgg ccagctccac ccggtcccgc cccgcctcct tggcccggta
     2641 catcgcccgg tcggcagccc gcaggagcgc gtcgaccgtc gtgccgtcct ccgggtagga
     2701 ggccaccccg acgctcacgg tgacgtggac gcccgccccg actccctccg cctgcaccgg
     2761 gcgaagggag atggcctggc gcaccttctc ggccacgact cgggcgccct cccgaggggt
     2821 ctccggcagc agcgccacga actcctcgcc tccgaaccgg gccaccacgt cgatgttgct
     2881 gcgggtcgag tccacgatcc ggcggacgac ctcgaccagg acctcgtcgc cgacgaggtg
     2941 gccgtgctcg tcgttggtcg ccttgaagcg gtcgatgtcg acgaggagca gggagagcgg
     3001 ccgcccgaag cgctgggccc ggtcgatctc cttctggagc tggagctgga ggaacctccg
     3061 gttccacacg cccgtcagcc cgtcggtcag ggacagacgc tcggcctcct ggtggaggag
     3121 gacgttctcg atcgccaccg aggcctggcc ggcgaacgag gcgagcgtcg ccgcgtcgtc
     3181 ctcggtgaag gggtcgggga gcgtgcggcc gtagagcgcg agcacgccga tcgtgctcgt
     3241 gccgcggacg agcgggacgg cgatggctgt ggtggccggg ggctcggccg gcgaccgctc
     3301 cgggatctcc tccaggcggg acggcacgag gacgggacgg ccctccgccg ctcggccggc
     3361 gatccccttc cccaggcgca tctcggtggc cgccgacggc tcgtacccca ggaccgcctg
     3421 gagccgcagc cgcttccccg agggcgtgag gaggaacagc gcggcggtgg acgcgtgcag
     3481 cgtcgcggcg gccgtgtcca cgatcacgct cagcatcgca tcgaggtcgt gggtggaccg
     3541 gagcgtggcc ccgagccgat cgaggttccg gcggagctca tcgcgcgact cctccagccg
     3601 ggtcacgtag gtctcgaggt tctcggtcat ctggttgaag gcgtcggcga ggttcccgat
     3661 gtcgccgcgg tccccgacca tcacgcgggt gtgcaagtct ccggcggcga cccgacgcgc
     3721 accctcggcg agctcgtaca cgggcctccc gatcacgccg gcaagcaggt agcccagcat
     3781 gctcgcgatc agcagcccca gcacggcgac cccccagacg gcgccgtgga cgcccgccac
     3841 ctcctcgggc gtcacgagca cgatctcgcc ggtctgccca cggatctggg tcaccgtgac
     3901 acgacggccg tcatcgacgg agacggggaa cggcccccgg gcggggaggt ctccgaggga
     3961 ggcctccgtc gcggccagca cccgcatccc ggatcgaagg atcagatcga cgtccgccgg
     4021 tcccacgagc tctctcagga actccgtgtc cacgaaccag cctccgacga cgccccccag
     4081 gttgcccgac acggagatcg gagcccgggc ccggagcagc cccggcacgc tgcgctcgcc
     4141 cacgatgtcg aaggccgtgg ggggagcaac cccccgggcg aacaacgggg accccatcga
     4201 cgatgcgatg atgccgccct gagggttcgt gacgacgagg aaatcgagac ccgcggcgga
     4261 gcgctccacc tcgagtcgct catcgagcgc tgtgccgcgg ccggggagga gctcggccgc
     4321 cacggacgcc gcgcggccgg tctcccgctc ggccacggtc aggcgctcct gccagagggt
     4381 cgagagcgtg gcggaggcgg cctcgagccg ggcgtcggac cgacggcgca cctcctgggt
     4441 aaccaggatc tggaggacga gcgtggccgc gacgaggggg acgacgacga tgccggcgaa
     4501 gaacagcgtg aggcgagcgc gcagggtcac ggcagggccc ctcgcgcgtt ggcttcccgg
     4561 gatgagcgac gcgcgcgcat cggttctctc cctttcgttg tcggcacctg ctcacccacg
     4621 acttaatcag gagcgaccgt gaccgacgat cccgtcaagg ccgccctccg tgcggagatg
     4681 cgccgcgtgc gggcggcgat cccggagggc gagcgt
//
LOCUS       DASUTW010000269        19601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_88582, whole genome shotgun sequence.
ACCESSION   DASUTW010000269 DASUTW010000000
VERSION     DASUTW010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 19601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19601
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88582"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..153)
                     /locus_tag="VF058_09000"
                     /pseudo
     CDS             complement(1..153)
                     /locus_tag="VF058_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513969.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 6 protein"
     gene            399..1019
                     /locus_tag="VF058_09005"
     CDS             399..1019
                     /locus_tag="VF058_09005"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX6208478.1"
                     /translation="MEHEAFNPVVCDLDGTLVDSGPAIIATVRLACAEVGAPVAEDRD
                     LGFCVGPPLEETLPMLVGDTELAGRAAEAYRALHPAVLSEGSSPAHPGAREALASFAG
                     AGISVAVATYKLTSLATATLEAGRLAGLVDIVVGRASPHDRRSKADLIVEALRLLAPH
                     RSKPVYIGDHADDEIAAAAAGVTYLRFGPLTWGDLELRVLSGESGQ"
     gene            complement(1052..1846)
                     /locus_tag="VF058_09010"
     CDS             complement(1052..1846)
                     /locus_tag="VF058_09010"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX6208479.1"
                     /translation="MKELRLLDLSQDFGMHTPGFAGYAGPQIRWIKRLAFETVGGQEL
                     CTTLHVGTHLDAPIHFFSGGKTIGELDLQWLVGPACVVDLERMGVGDYDLYGPEEFER
                     WERDTGLSIDRGDILIVHTGYHRFYPDNWTDRSQVDETRYFIRHPGPTREFAEWILDR
                     GVRWMAIDAGSQDHPMNTVIRKIRADEAEDAEEKLGRPLADIWPKKDYQIMHTLLFPH
                     NVIHIENLGGDIDKVLDQKVMFGCFPWRFQGGEAAFCRAVVFLDGA"
     gene            complement(1896..2696)
                     /locus_tag="VF058_09015"
     CDS             complement(1896..2696)
                     /locus_tag="VF058_09015"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HEX6208480.1"
                     /translation="MVVLSLNTVAPAAMHALAASREGRVLGWGPRAGYVQLDSFVLAV
                     NPAGTPLMPNGVTVEVATLPPISGRQTWVDAGLIRTHGLSLVWDPTAPPVWDPRVSSQ
                     VVTRETVEARGRAIAEITGRLRLDVHPAALEAGGTLRQGADVLVAAREMLGRGPGLTP
                     EGDDVLAGAVATVRAFGGACGLDPDRWVAPFEELDLDGFTTGLSATLLRLALRGHVIQ
                     PVRPLLDLSRQASSSDVEKLLRVGHSTGRAYAAAIAGTAAALAATAPG"
     gene            complement(2696..4105)
                     /locus_tag="VF058_09020"
     CDS             complement(2696..4105)
                     /locus_tag="VF058_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HEX6208481.1"
                     /translation="MTDGGILGRHPRVANIGVDVFADEMEGQGVEVTRVDWRPPAEGS
                     ETALALLSRAAPQVAAGNDEVIARLQGSRPLLVGVGTAGDVIPGMDHSTLLHAGPPIE
                     WVDMSGPVRGAVIGAAIHEGLAATPQEAERLAASGAFRFSPTHEHCAVGPMAGIVSAS
                     MPVWIVENDPRGNRAYCTMNEGLGKVLRYGAFDREVLDRLAWIRKVLAPLMQDAIAGL
                     GEPLDLRSLIAQALQMGDEGHNRNRAGTSLFLRELFPSLLALDAPSADKIAAVRFVSD
                     NDHFFLNLTMPAAKATADAAAGIRESSIVVTMTRNGTEFGVRLAGTGDRWFTGPAGIV
                     EGLFLPGFGPEDANPDLGDSVITETVGLGGLAMASAPAIVNFVGGSAADAVDATTSMY
                     GITWSESAHYRIPALDFRGTPLGIDCREVVHTGVLPVINTGIAHRDPGIGQVGAGVVR
                     PPMRAFVEALSALAEAAVP"
     gene            complement(4102..5553)
                     /locus_tag="VF058_09025"
     CDS             complement(4102..5553)
                     /locus_tag="VF058_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdrA family protein"
                     /protein_id="HEX6208482.1"
                     /translation="MRISKEVGGRAGVLAAAVVMGTPLNVGLVGDLGFELPDAGPNDL
                     LVAIRAGSAEDADAAVATAEALLAERAPHGVDGGVVAAPRSIAAAARQDSGLSIAFVS
                     VPGAHAAYELASALENGLHVFCFSDGVGLLEEATLKQLAAERGLLLMGPDCGTAIVDG
                     VAFGFANAVRRGPVGVVGASGTGTQELCCLLDMAGVGVSHAIGVGGRDLSSEVGGIMT
                     LRALELLASDPATSVIVVVSKPPDTAVADRVVADAARTSRPAVVCFLGTDRPSSGLPN
                     GVEVTGSLEEAARMAADLAGRPLIPSADPQPETVTAGDIRGLFTGGSLCDEAMALVAR
                     RVGRVASNTPLRPDWALEDVRRSEGHTFIDFGDDRLTEGRPHPMIDPTIRNRRFLEEA
                     ADAAVGVVVLDVVLGFGAHPDPASELGPLVEGALDGRAGTLSVVVDVCGTPADPQDVT
                     AQAARLGEAGALITRSAAQAARTALRAAGPVDR"
     gene            complement(5604..6878)
                     /locus_tag="VF058_09030"
     CDS             complement(5604..6878)
                     /locus_tag="VF058_09030"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6208483.1"
                     /translation="MTTVDLILRRARLEQRDGIVDIAVEGGAIAAIAPRVPAEAPVEL
                     EAEGRLVSPAFVDPHIHLDKVEVFPLLPPNRTGTLSEAIELLHRTKRAASVEEIAARA
                     GAVIRRAVAAGTTFIRTHVDVDTVGGLRPLEGVARARAEHADLCEIQIVAFPQEGIER
                     DPGTEDLMREAMERGADVVGGMPHWERTPDASQRHVDVCLDLAVRHDADVDMHIDETD
                     DPRSRTLEVLIDATESYGWGGRVAAGHVCAMAGWDDGYAADMVARAASAGVALITNPC
                     TNLLLQGRMDNEPRRRGLLRVKEALAAGVVVGCGQDCVNDAFYPFGAADQLQVALVLA
                     HAAQLSTPSEIAAALRMVRHDAARILRLERYGVAEGSQADLVVLDAEDLHEALRYQVP
                     RRWVIRRGRVAAETVTGRRLHREPMADAAADA"
     gene            complement(7212..7550)
                     /locus_tag="VF058_09035"
     CDS             complement(7212..7550)
                     /locus_tag="VF058_09035"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX6208484.1"
                     /translation="MADSVVAARCEKCGKLSYPTHFSCPSCGGTSFESTPVEGGGTLL
                     TWTRVYALPLDYADLFITLGIVEMDSGIRATGRLEIDEPRTGARVRASVGKVREIGGK
                     TISGLVFRTA"
     gene            complement(7552..8736)
                     /locus_tag="VF058_09040"
     CDS             complement(7552..8736)
                     /locus_tag="VF058_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HEX6208485.1"
                     /translation="MRDVAIVGIGSTQFGRAQWSLLKLMAEASSEAINDASLGERSID
                     AVYVGNMGSVRNNRQAGIASALVDRLNLLPAAADAVENGPASGASAVKNGFLAIASGF
                     YDLVLVCGGERMQEVNNFETTEFVSYLSHRYAEYVYGVTLPALAGMFARLYMERYDVH
                     PEHLAMVAIKNHANGVLNSRAHLRTPVTMEGLITGPDAMMNNPWIADPLRFFDCCPVS
                     DGASAVLLAPVEIAGELTDRPVTIAGIGQATDTHSVHERPDPTELTAVHVASEKAFAM
                     ARLEPSDIDVAELHDAFTILEIAESEQAGFFPKGEGHKALEAGDTEIGGRLPINPSGG
                     LKARGHPVGATGVSQVVEVARQLRGDAGDYQVSGSPEHGFALNFGGFGNNVLATVLKR
                     GV"
     gene            complement(8733..9422)
                     /locus_tag="VF058_09045"
     CDS             complement(8733..9422)
                     /locus_tag="VF058_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208486.1"
                     /translation="MIDELEAMLRPPALPMAEYPPKSILLASGEPMVVRQASIEEVDA
                     MLRYIQPLVWLNRDYYDIVAARLFAELLGWRRYRVRNEYCLVGVIDGVLAGIVNGRMF
                     DEKHGVSYHTLALRRGLRIGAHLFASKMEHHIEFLGQEEVWIVAESPIGHRRWMIEYP
                     LAPKSEIQHELGGASSWVLSRDTYFAAKPRLVSGTRPVSEEQLASSYPIGEPDLQTML
                     VQIEDTLEARI"
     gene            9658..10284
                     /locus_tag="VF058_09050"
     CDS             9658..10284
                     /locus_tag="VF058_09050"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX6208487.1"
                     /translation="MADLLRDEIRRGELAPGARLRQEEVAGRLGVSTTPVREAFQALQ
                     AQGLLTLDPHRGVVVFQPTVQDVRESYEIREALETLVISQAVPLLTTDDLRELQAIVD
                     EMKVADNDERWIDLNTRFHSRLNAASGRKRLCEMIEGIRNATSAYMNMVVPNARASGR
                     GDSGHEEILRACRKGDVEAAVGAMRSHLNHTVQQTLRIIGNEQETAGS"
     gene            complement(10281..11699)
                     /locus_tag="VF058_09055"
     CDS             complement(10281..11699)
                     /locus_tag="VF058_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX6208488.1"
                     /translation="MPDLDHRHHLVLRGGVVVTMDAGRTVLDRGDVAVAGGRIVAVAP
                     GGQIPDAAGGRVVDCSGCAVLPGLVDCHNHLFQGLCRGLGDGLALWDWLTRLIWPYAD
                     HVTPEDARAAARLAAVEAVRAGTTSLLDNHYAPADPASTLAVAEAMEEVGLRGVVARG
                     IFGPRTDVARDRGIPDLLFGRSAEEELEATAACLDARPAGGRVEVWPAPINPTYVEPD
                     LLVRAVRLARDARVRWHTHCAEVRADVATSMGTHGVRPLVRLAREGLLEGGTVAHGIW
                     LDEEEIMHLAQAGAAVSHCPVANQYLASGVAPVGELRRAGVAVGLGTDGPAGGQRQDL
                     FECMKSSVLLQRVHALDPSAAQAEDALEIATIGGAALLGIDGGSLEPGKLADLVVVDL
                     RGPHLRPLHRAATALAYSARGADVRMTVVGGEVVFEDGRCTRVDEGEVLVEAQARAEA
                     LVDRAGLSHLRAVWRPPSGPPG"
     gene            11717..13999
                     /locus_tag="VF058_09060"
     CDS             11717..13999
                     /locus_tag="VF058_09060"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6208489.1"
                     /translation="MPGPDSSGSAIGLGLPRAEDAELLNGSARFVGDLLPDGCLHLAF
                     VRNREPHARLLGVSLERARAAPGVATALAAADLGPSSSMPSAPWLGLPEAFSRPVLAR
                     DRVRFVGEPVAVVVAEHRARGEDAAELAEVALEPLPPVLDPTEGADSASPRLFESESN
                     VVLEERAGHEDAGAAVMNAPVVVEGTFRIGRLAHLSLEARAVLAVPEPRGGLTVWCSH
                     QAPHRLRSGLAAALGLDAGLIRVRVPAVGGAFGGKSQTYPEYVLACRLALELGRPVRW
                     VEDRREAFLAAVHGRGSNIRLRVGAARDGTLLALETAIDGDLGAYPETGALVPTFAAW
                     LISGPYRIPRLSSVVRGVVTNKPPTAPYRGAGRPEAAYVLERSIDLLADRLRLDPGEV
                     RRRNLIPADAFPYASPTGAVYDSGDYRRALDRALELAAYEELRRDEAWRLGDDLGGRV
                     VRGVGMAVYVERSGGTSDLADYARVDLEPDGTLTIRSGACSHGQGHRTALAQVAAAAL
                     EVDLDRVRVVQGDTAEVSSGGGTFGSRSMQIGGAAVHLACCEFIALAIRRAAEALGTD
                     RASLQYEGGMVRSAGTELPLEDLACRTGPLSAESRFSGQQAFPFGAYVATVDIDRETG
                     AVRVTRLVAVDDCGVVVNPLLLEGQIQGSIVQGLGQALTEEVGYEPDGRPTTATLFSY
                     VTPGPVELPDLVLDRTVTPNPNVPTGAKGGGEAGTIGAPPAIVNAIVDALRGYDTSVI
                     TMPVTPEVVWKALSSPRRVG"
     gene            complement(13974..15329)
                     /locus_tag="VF058_09065"
     CDS             complement(13974..15329)
                     /locus_tag="VF058_09065"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HEX6208490.1"
                     /translation="MSFDLAIRGASVWREDGTFRRLDLGIREGKVVALAVPAWDFPSA
                     GRVVDAEGRLLLPGLIDTHVHLRDPGFTYKEDYETGTRAAAAGGVTMVVDMPNTDPVP
                     NTVERFRAHRANAASKSLVDFNHWASPTVPEEIRGIAREGAMGFKFFMISHHYPYDNP
                     EQFISIAEPHRIYRTMVEIAATGRPCLVHPHNQSMWQELTREFVEAGTTLPKHREAAY
                     TYAGNFVQNSAIATVMLLADVTRCRLRVLHNNYPPVIRLVRGMKAAGYQAVMEQNPWA
                     IFGKGIPDHTIDEGPIWESLNDGTIDLLASDHAPHSTDELLESRADAFNSIVSSLTIV
                     EHMLALFLTRIAEGAPLTVHRLVQLFSTNVAKHLDVYPRKGTIRVGSDADLVLVDLDE
                     RGIVDSTKLYSKCGNTPYEGWPLRGMPVMTVAGGRVVMEDRVVTAEPGSGRFIPPDGA
                     S"
     gene            complement(15326..16324)
                     /locus_tag="VF058_09070"
     CDS             complement(15326..16324)
                     /locus_tag="VF058_09070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6208491.1"
                     /translation="MPDGTPRFGILLPHFGPHANRDRIIERSAEIERLGFDSIWVRDH
                     VVFRPHGHEGGDSTFWEPTLALAAIAAVTSRVTLGTGALIPYRHPLHTALLFGTLDQL
                     TGGDRLILGFGLGSFDHEFEAVGMGAWDRRKVLPEQVEIMRKVWREDGVSHQGEFYSF
                     SDVAISPKPRSGAVPVWYSGSSPASVRRAVEFCDGWVPGRMPRRDFRKRVERMTRLAQ
                     EAGRRVPATGTIPYVSPGRTMEEATAGLHLDALIEETAKRYPPGESGVFESLEDLDGA
                     LVAGPPDVLVEEVVKHVESGVQHFVFDMRLRFDQYEEMLDLIAEDVLPEVRRICAV"
     gene            16366..16902
                     /locus_tag="VF058_09075"
     CDS             16366..16902
                     /locus_tag="VF058_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfopyruvate decarboxylase"
                     /protein_id="HEX6208492.1"
                     /translation="MGPPPDRPGRERSAGPAATIVEGLKGAGVSLAAYVPDSLLRSTF
                     RLLREDPDIRTVQTSNEADLPGIVVGAYLGGVRAVLMMENSGIRQGCEPIARFTYSYR
                     FPMVMLLSHRGGFPEPTWWGHAHAQTMAPILDALRVPWRLLETPEEVGPAVAGAFTHS
                     RSSQWPVALVLSGECLER"
     gene            16904..17545
                     /locus_tag="VF058_09080"
     CDS             16904..17545
                     /locus_tag="VF058_09080"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6208493.1"
                     /translation="MQRRDVLSTLARLVTPEDLVVTWRSTTWDDWAELAPAGARNTLP
                     LAILGSISTTALGLAIALPYRRVVAVESDGSVLMNAGAMATLGAERPPNLTVVVLDNG
                     MYESIGGPPTLTARHADLARMAEGAGCPNSLAVDGQAALEEAAARLLDDGEMGYLVAR
                     IKPGRPAGTPRTPMTWPRTDGVEDKYRFLRYVEELEGIVIHPDPGPGPGRVGG"
     gene            complement(17542..17934)
                     /locus_tag="VF058_09085"
     CDS             complement(17542..17934)
                     /locus_tag="VF058_09085"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6208494.1"
                     /translation="MSEEAPSEGKVVDPDTAPRRDMQLERGYAIQLVDPNLGAERLDM
                     HINVLKARGAPGPYHLHTNCENIYFILEGDVVVTIDGVAHQMKPGMAVFIPPNVPHSA
                     SNVGDTEARLVEIYTPPGQDFVHVAKEG"
     gene            complement(18237..18956)
                     /locus_tag="VF058_09090"
     CDS             complement(18237..18956)
                     /locus_tag="VF058_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017728907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX6208495.1"
                     /translation="MGWRELIPPADLEVYGVAGYGGRMELGRRPACLVIDVTYGFVGK
                     PGLSLRDAIRVHPNACGPSGWKAVPRIREVLDAARAASIPVFYTAGMSRHVEENLGRW
                     RDKHRRTLERPPDTQVIVEEIAPGPGEVLIEKTKPSAFFGTPLTATLIDLGIDSVIVA
                     GCTTSGCVRASVTDAFSYGFRVAVVEDGTFDRAELPHAVNLFDMDSKYANVMPAADVA
                     AYLRSLQPGRDADMGTDVATR"
     gene            complement(18961..>19601)
                     /locus_tag="VF058_09095"
     CDS             complement(18961..>19601)
                     /locus_tag="VF058_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6208496.1"
                     /translation="VVLAFKELAIDSRVLWTALADSLTVLVIGFSAAIVVGTLFGVLM
                     GRFRLLEYVLDPYVSFLYALPTVVIMPLLIIWIGIGSTTRVAIVFSTSLVPVLLNTMA
                     GAKLVSQDLIDVARNYGATNRQTLRSIVVPAILPYFFAGLNVASGTALIGMVLGEMLL
                     VIRGLGGLIVEYSNFFRTDRMFVALLAIVALSVAFRSGIRRLRGALMPWSEV"
BASE COUNT         3095 a         6948 c         6550 g         3008 t
ORIGIN      
        1 ggactccccg ggcgtcttca cccacaggta cgcgtcgacc agcgggttgc cggtgtccaa
       61 ggtcggcctc ggcccgaggc cccggcccgg cgggttgcac cagtcctggg gatccgggta
      121 ggcaggaggc gcccacggcc ccaggccgtt gcggcggttc gttacctggg ccagccgacg
      181 gcctgctctg ggaaccagtc gcctgcggcc gtcgatccga accgcctctc ggcccaaacc
      241 gcctctcggc ggcccagcgg agcgctcact attggctgtg atgttccggc tccgatgagc
      301 gaacgcagcg acgaacaatc ccttgggttc taacgctttg gcgtagccag atgcccgatc
      361 ctcgtaacgt ggcaggaact ggaaggaggt gccgcctcgt ggagcatgaa gcgttcaatc
      421 cggtggtctg tgatctggat gggacacttg tcgactccgg gcccgccatc attgccacag
      481 tccgcctagc atgcgcggaa gtcggggctc cggtcgccga ggaccgcgat ctgggtttct
      541 gtgtgggccc tcccctggag gagaccctac cgatgctcgt cggcgacacc gagctggctg
      601 gacgggcagc tgaagcctac cgggctcttc accctgccgt cctgagtgag ggctcctccc
      661 ccgcgcatcc tggagcaagg gaggcactgg cctcgtttgc aggagcgggt atctccgtgg
      721 cagtcgccac atacaaactg acgtcgctgg ccacggccac tctggaggca gggaggctcg
      781 ccggtctcgt ggacatcgtg gtcggtaggg catccccaca cgatcgtcgc agcaaagccg
      841 acctgattgt tgaggctctt cgacttctgg ctcctcatcg cagcaagccc gtgtatatcg
      901 gcgaccatgc ggatgatgag atcgccgctg ccgccgcggg tgtgacctac cttcgtttcg
      961 ggcctctgac gtggggtgac ctagagctac gcgtcctgtc gggcgaatcg ggccagtgag
     1021 ccgagaccgt agccggcgca gaccgaccag cttatgcccc gtccaagaac acgaccgcgc
     1081 gacagaacgc ggcctcaccc ccctgaaacc gccaagggaa gcacccgaac atcaccttct
     1141 ggtcaagtac cttgtcgatg tcaccgccga ggttctcgat gtggatcacg ttgtgcggga
     1201 acagcaaggt atgcatgatc tgataatcct tcttcggcca gatgtcggcc agcgggcggc
     1261 ccagcttttc ctcggcgtcc tctgcctcgt ccgcacggat ctttcggatc acggtgttca
     1321 tcgggtggtc ctggctgcca gcatcgatgg ccatccatcg gactccgcgg tcaaggatcc
     1381 attccgcgaa ctcgcgggta ggtccggggt ggcggatgaa gtaccgggtc tcgtccacct
     1441 gactccgatc ggtccagtta tccgggtaga agcggtggta tccggtgtgc acgatgagga
     1501 tgtcgccccg gtcgatgctg aggcctgtat cacgctccca acgctcgaac tcctcgggcc
     1561 catagaggtc gtagtccccg actcccatcc gctccaggtc gaccacgcac gccggcccca
     1621 ccagccattg caagtccagc tctccgatgg tcttcccccc ggagaagaag tggatcgggg
     1681 cgtccaggtg cgtaccgacg tgcagcgtcg tgcacaactc ctgcccacca acggtctcga
     1741 aggccaatcg cttgatccac cggatctggg gcccggcgta cccggcgaag ccgggcgtgt
     1801 gcatcccgaa gtcttgggac aggtcaagca gccgaagctc cttcatccat tcacctcccc
     1861 gggctctcgc ccgcttcgca tcgtcatgga tacagtcatc ccggcgcggt cgcggcaaga
     1921 gcagccgccg tgcccgcgat cgcggccgcg tacgctcgcc ccgtcgagtg accaactcga
     1981 aggagcttct ccacatccga gctcgacgcc tggcgggaca ggtccaggag cgggcggacc
     2041 ggctggatca cgtgtccgcg gagggccaac cggagcaggg tcgcggatag accggtcgtg
     2101 aagccatcga gatcgagctc ctcgaacggg gcgacccatc gatccgggtc caacccgcag
     2161 gcgccgccga aggcccgcac ggtcgcgacg gcccctgcga gcacgtcgtc accctcgggg
     2221 gtcagtccgg gtccccgacc cagcatctca cgggccgcga cgagcacgtc agcgccttgg
     2281 cgcaaggtcc cgccggcctc caacgctgcc ggatggacat cgagccggag cctccccgtg
     2341 atctccgcga tcgcccgccc tcgggcttcc actgtctcgc gtgtaaccac ctgggagcta
     2401 acccggggat cccagaccgg gggcgccgtg ggatcccaga ctagagagag tccgtgcgtc
     2461 cggataagac ccgcgtctac ccacgtctgg cggccggaga tcgggggaag cgtggctacc
     2521 tcgacggtca ctccgttggg catgagcggg gtcccggcgg gattcacagc caacacgaac
     2581 gagtccagct gcacataccc ggcgcgtggc ccccacccca gcacgcgccc ctccctgctg
     2641 gcggccagag catgcatggc cgccggggcg actgtattca gggacaagac caccatcagg
     2701 gcaccgctgc ttcggccagg gcgctcaggg cttcgacgaa cgcccgcatc ggcgggcgaa
     2761 ccacgccagc accgacctga ccgatgccag gatcgcggtg ggcgatgccc gtgttgatca
     2821 ccggcagcac gcccgtgtgg accacttcgc ggcagtcgat accgaggggg gtcccccgga
     2881 aatcgagcgc ggggatccgg tagtgcgcac tctccgacca cgtaatgccg tacatcgagg
     2941 tcgtcgcgtc gaccgcgtcc gcggcggagc cccccacgaa gttgacgatg gcgggagcgc
     3001 tggccatggc cagcccaccg aggccgacgg tctcggtgat cacgctgtcg cccaggtcgg
     3061 ggttggcgtc ctccggaccg aaccctggga ggaacagccc ctcgacgatc ccggcggggc
     3121 cggtgaacca gcggtccccg gtccccgcca gccggactcc gaactccgtt ccgttcctgg
     3181 tcatggtcac cacgatcgag ctctcgcgta tgccggccgc ggcgtcggcg gtggccttcg
     3241 ccgcgggcat ggtcaggttc aagaagaagt ggtcgttgtc gctcacgaac cgcaccgccg
     3301 cgatcttgtc ggcggacggg gcgtcgagcg ccaggagtga cgggaacagc tcgcggagaa
     3361 agagtgaggt tcccgcccgg ttgcggttgt gcccctcgtc gcccatctgg agggcttgag
     3421 cgatcaaaga tcgcaggtcc agcggctctc cgagcccggc gatcgcatct tgcatcagcg
     3481 gggcgagcac cttccggatc cacgccaggc ggtccaagac ttcccggtcg aacgccccat
     3541 agcggaggac cttcccgagc ccctcgttca tcgtgcagta cgctcggttc ccccgcgggt
     3601 cgttctccac gatccacacc ggcatcgatg cactgacgat cccggccatc ggccctacgg
     3661 cgcagtgctc atgcgtggga gagaagcgga atgcacctga ggcagcgagc cgctcggctt
     3721 cttgaggcgt ggcggccagg ccctcatgga tcgcagcgcc gatcaccgcc ccccgcacgg
     3781 ggccggacat gtcgacccac tcgattggcg gaccggcatg caagagagtc gagtggtcca
     3841 tgccggggat gacgtcgcct gccgtgccca ccccaacgag gagcggacgc gatccttgga
     3901 gccgggcgat cacctcgtcg ttccctgcgg ccacctgagg ggccgcgcgg gagagaagcg
     3961 ccagcgcggt ctcgcttccc tcggcgggag ggcgccaatc gacccgggtc acctcgacgc
     4021 cctgaccctc catctcgtcc gcaaacacgt ccaccccgat gttggcgacc ctggggtgcc
     4081 taccgaggat tccgccgtcg gtcatcggtc cacgggtccc gcggctcgga gtgccgtacg
     4141 cgctgcctga gctgcgcttc gggtgatcag tgcccccgcc tcaccaagcc gtgcggcctg
     4201 ggccgtgacg tcctggggat ccgccggcgt cccgcaaacg tccacgacaa cggagagtgt
     4261 ccccgcgcgc ccgtcaagag caccttccac caacggaccc agctccgacg caggatcggg
     4321 gtgcgcgccg aaccccagga ccacgtccag caccaccacc cccaccgccg cgtcagccgc
     4381 ctcctcaagg aaccgacggt ttcttatggt cgggtcgatc atcgggtggg gacgaccttc
     4441 ggttagccgg tcgtcgccga agtcgatgaa ggtatgtccc tcgcttctcc tcacgtcctc
     4501 gagagcccaa tcagggcgaa gaggggtgtt cgaggccacc cggccgaccc gtcgagccac
     4561 caatgccatc gcctcgtcgc agagagaccc gcccgtgaac aatccccgga tatctcctgc
     4621 ggtgaccgtt tccggttgag gatcggcgct ggggatgagc ggccgcccgg caagatccgc
     4681 ggccatccga gcggcttcct ccagcgaccc ggtcacctcc acgccgttcg gcagcccgga
     4741 ggatggccgg tccgtgccaa ggaagcacac caccgcgggc cgtgaggtcc gcgcagcatc
     4801 cgcgacgacg cggtccgcca ctgccgtgtc cggcggcttg gacaccacca cgatcacgct
     4861 cgtggcggga tccgacgcga gcagttccag ggcccggagg gtcatgattc cgccaacctc
     4921 ggaagagagg tcccggccac cgacgccgat cgcgtgagag acgcccacgc ccgccatgtc
     4981 gagcaagcag caaagctcct gggttcctgt tccggaggca cccactaccc ccacggggcc
     5041 ccggcggaca gcattggcga agccgaaggc cacgccgtcc acgatggccg tcccgcaatc
     5101 gggacccatc aagaggagcc cccgctccgc ggcgagctgc ttcagcgtgg cctcctcgag
     5161 cagacccacg ccatcggaga agcagaacac gtggagaccg ttctccaggg cggacgccaa
     5221 ctcgtatgcg gcgtgagccc ctggcacgga gacgaacgcg atcgagagcc cagagtcctg
     5281 tcgtgcggct gcagcgatcg agcggggagc cgcgaccacg ccgccgtcga ccccgtgggg
     5341 ggcccgctcg gccagcaggg cctccgcggt tgcgaccgct gcatcggcgt cctcggccga
     5401 cccggcccgg atggccacca agagatcgtt gggcccagcg tccgggagct cgaagccgag
     5461 gtcaccgacc agcccgacgt tgaggggcgt gcccatgacc acggcggccg caagtacccc
     5521 ggcccgcccc ccgacctctt tcgagatccg catcagggca acggagtcgc gataggtatc
     5581 cggcaggacg acgacccgtt cggtcatgcg tctgccgccg cgtccgccat gggctcccgg
     5641 tggagacgtc gtcccgtaac cgtctcggcc gccacgcgac cccgccggat cacccatcgc
     5701 ctcggaacct ggtagcgcag agcctcgtgc aagtcctctg cgtcgagcac gacgaggtcc
     5761 gcctggctgc cctccgccac gccgtaccgc tcgaggcgga ggatacgcgc cgcatcgtgg
     5821 cggaccatcc ggagggcggc ggcaatctcg gacggcgtgg agagctgcgc ggcgtgggcc
     5881 aggaccagtg ccacctggag ctggtccgcc gccccgaacg ggtagaaggc atcgttcacg
     5941 cagtcctggc cgcagccaac caccacgccc gctgccagcg cctccttcac ccggaggagc
     6001 ccgcgccgac gcggctcgtt atccattcga ccctggagga gcaggttcgt gcaggggttg
     6061 gtgatgagcg ccacacctgc cgatgcggcg cgggcgacca tgtccgctgc atagccatcg
     6121 tcccaccccg ccatggcgca tacgtggcca gcggcaaccc ggccgcccca tccatacgac
     6181 tccgtggcgt cgatcagcac ctccagggtc ctcgatctag gatcgtcggt ctcgtcgatg
     6241 tgcatgtcca cgtccgcgtc gtggcgaact gcgaggtcca ggcacacgtc gacatggcgc
     6301 tgggacgcat cgggtgtccg ctcccagtgg ggcatccccc caaccacatc cgccccacgc
     6361 tccatcgcct cgcgcatcag atcctcggtc ccggggtccc gctcaatgcc ctcctggggg
     6421 aacgccacga tctggatctc gcacaggtcg gcgtgctcgg cgcgggcccg ggcgactccc
     6481 tcgagcggtc ggaggccgcc cacggtgtcc acgtcgacat gggtgcggat gaacgtggtc
     6541 cccgcggcaa cggcgcgacg aatcacagcg cctgcgcggg cggcgatctc ctccacagag
     6601 gccgctcgct tcgttcggtg aagcagctcg attgcctcgc tcaaggtccc cgtgcggttc
     6661 ggcggaagca gagggaaaac ctcaaccttg tcgagatgga tgtgggggtc cacgaatgcg
     6721 ggactgacca aacggccctc cgcctcaagc tcgacgggtg cttccgcagg cacgcgcgga
     6781 gcgatcgcgg cgatggcgcc tccctcgacc gcgatgtcca caatcccatc gcgttgctcg
     6841 aggcgggctc gccggaggat caggtccact gtcgtcaccc gaaccgatac aaccggaggc
     6901 cgttgtccac cagtaccctt ccactcgcct cctccccagg cgagccgggg atcaggtcga
     6961 cttgagtatc ctcggacggg cgccgcgtcc gggctgacac gacgtagccg tcgtcgtagc
     7021 cttggattga gcccgttacc acggcgccgg ttgccagcgc atacctcctc gcatgaccca
     7081 gtagtcctcc acggtgaccg gctccggcgc gaacctctcg ctgcaccgga gcacgaaccg
     7141 cggttcgacg atgtggaagt gcccgtcgaa caggcaccgg cccatatctc ccgcgggctg
     7201 cggccgcgac gtcacgcggt tctgaacacc agaccgctga tcgtcttgcc cccgatctcg
     7261 cggaccttcc ccacgctggc cctcacccgc gcccccgtcc tcggctcgtc gatctcgagc
     7321 ctgccggtgg cccggatacc cgagtccatc tcgacgatgc cgagcgtgat gaacaggtcc
     7381 gcgtagtcga ggggcagcgc gtagacccgg gtccaggtca ggagcgttcc gcccccctcg
     7441 acgggcgtgc tctcgaagct ggtccccccg caggagggac acgagaagtg ggtcgggtag
     7501 gagagctttc cgcacttctc gcacctcgcg gcgacgaccg aatctgccat ctcacacccc
     7561 ccgcttgagc acggtcgcga gcacgttgtt cccgaagccg ccgaagttga gggcgaaccc
     7621 gtgctcgggc gagcccgata cctggtagtc gccagcgtcc ccgcgcagct ggcgagccac
     7681 ctcgacgacc tgggacacgc cggtcgcccc gaccgggtga cccctggcct tgagaccgcc
     7741 ggacgggttg atgggcagcc gccctccgat ctcggtgtcc ccggcctcga gcgccttgtg
     7801 gccctcgcct ttcgggaaga acccggcctg ctcgctctcc gcgatctcaa ggatggtgaa
     7861 ggcatcgtgc agctctgcca cgtcgatgtc cgagggttcc agccgggcca tcgcgaacgc
     7921 tttctccgac gcgacatgca ccgccgtgag ctcggtggga tccggccgct cgtggaccga
     7981 atgagtgtcc gtggcctgac cgatgcctgc gatcgtcacc ggccggtccg tgagctcgcc
     8041 ggcgatctct accggcgcaa gcaggactgc ggaggcgccg tcgctcaccg gacaacagtc
     8101 gaagaaccga agcgggtcgg cgatccacgg attgttcatc atcgcgtcgg gacccgtgat
     8161 cagaccctcc atcgtgaccg gggtccggag gtgcgcccgg ctgttgagga ccccgttcgc
     8221 gtggttcttg atggccacca tcgcgaggtg ctcgggatgg acgtcgtacc gctccatgta
     8281 caagcgggcg aacatcccgg caagcgccgg aagggtgacc ccatagacgt actccgcgta
     8341 ccggtgcgac aggtacgaga cgaactcggt tgtctcgaag ttgttgacct cctgcatccg
     8401 ctccccaccg cacacgagga ccaggtcgta gaaaccggag gcgatcgcga ggaagccgtt
     8461 cttgactgcc gaggcgccgg aggccggacc gttctcgacg gcgtcggctg ctgccgggag
     8521 caggttgagg cgatcgacca gtgcgctagc gatgccggcc tgacgattgt ttcggaccga
     8581 acccatgttg ccgacgtaga ccgcgtcgat cgaccgctcg ccgagggacg cgtcgttgat
     8641 ggcctcggac gacgcctccg ccatcagctt cagcaggctc cactgggccc ggccgaactg
     8701 ggtggacccg atcccgacga tggcgacgtc tctcatatgc gggcctccag agtgtcctcg
     8761 atctgcacaa gcatcgtctg caggtctggc tcaccgatcg ggtacgaaga ggccagctgc
     8821 tcctcggaga ccggccgcgt acctgaaacg agccgtggct tcgcggcgaa gtacgtgtcg
     8881 cggctgagga cccacgacga tgccccgccg agctcgtgct ggatctcgga cttcggggcc
     8941 aacgggtact cgatcatcca tcggcgatgc ccgatcgggc tctcggccac gatccagacc
     9001 tcctcctggc ccaggaattc gatgtggtgc tccatcttcg aggcgaatag gtgcgcacct
     9061 atccgcagcc cgcgcctgag tgcgagggtg tggtagctga cgccgtgctt ctcatcgaac
     9121 atccgcccgt tcacaatgcc ggcgaggacc ccgtcgatga cccccaccag gcagtattcg
     9181 ttgcgcacgc ggtaccgcct ccacccgagc agttccgcga ataggcgtgc agccacgatg
     9241 tcgtagtagt cacggttcaa ccacacaagc ggctggatgt atctcagcat cgcgtcgacc
     9301 tcttcgatcg aggcttggcg gacgaccata ggctcgccgc tcgccagcag gatcgacttg
     9361 ggcgggtact cagccatcgg caatgccggg ggacgcagca tcgcttcgag ttcgtcgatc
     9421 atccgctcct cctcgtccgt cggggaacgc gtgcctcccc ggccccgggc cggcgaacgt
     9481 atattatgtc ggcgatatta gaggcatgtc gaccctgggg cgaaccggcg cctaccgtac
     9541 ggtggccttc tggagcggtg aagcagggtc cgcgtatagt atccgggctg gtcctcccga
     9601 ccggcggtcg gaggcgtcgt gggtaaggtt atcgagaggc ggaccacgag cgccgtggtg
     9661 gccgacctcc tccgcgatga aatccgccga ggggaactcg cgccgggcgc acgcctacgt
     9721 caagaggagg tcgccggacg cctcggcgtg agcaccaccc ctgtccgtga ggccttccag
     9781 gcgctacagg cacagggcct gcttaccctc gacccgcacc gaggcgttgt ggtcttccag
     9841 cccaccgttc aggacgtgcg ggagagctac gagatccggg aggccctgga gacgctggtc
     9901 atctctcaag cggtcccctt gctcaccacg gacgacctgc gcgagctcca ggcgatcgtc
     9961 gacgagatga aggtcgcgga caacgacgag cggtggatcg acttgaacac ccggtttcac
    10021 tcccgcctga acgcggcctc cggccgcaag cgactctgcg agatgatcga ggggatccgc
    10081 aacgccacga gcgcctacat gaacatggtg gtgccgaatg cgcgcgcctc ggggcggggc
    10141 gactccggcc atgaggagat cctccgggcc tgcaggaagg gcgacgtcga ggcggcggtc
    10201 ggggcgatgc gctcgcacct gaaccacacc gtccagcaga ccctgaggat catcgggaac
    10261 gagcaagaga ccgcgggcag ctagccaggc gggcccgacg gcggtcgcca gacggcccgc
    10321 agatgggaca gcccggcccg atccaccagc gcctcggccc gcgcctgtgc ctcgaccagg
    10381 acctcgccct cgtcaacccg cgtgcaccgg ccgtcctcga acacgacctc accccccacc
    10441 acagtcatgc gcacgtcggc acctctagcg gagtacgcaa gggcggtcgc cgcgcggtgc
    10501 agggggcgca ggtgcgggcc ccgaaggtcc acgactacca ggtcggccag cttgcccggc
    10561 tcgagcgacc caccgtcgat ccccaggagg gccgctcctc cgatggtggc aatctccagg
    10621 gcatcctccg cctgcgccgc cgacggatcc agcgcgtgta cccgctgcag cagcaccgac
    10681 gacttcatgc actcgaacag gtcctggcgc tgaccgccgg ccgggccatc ggtccccaga
    10741 ccgaccgcca cccctgcccg gcggagctcc ccgaccggcg ccacgcccga cgccaggtat
    10801 tggttggcca cggggcagtg cgacaccgcg gcgccggcct gcgccaggtg catgatctct
    10861 tcttcgtcca gccagatccc gtgggcgacc gtccccccct ccagcagccc ctcacgggcc
    10921 aggcgcacga gcggccggac gccgtgagtg cccatcgagg tcgcgacgtc ggcccggact
    10981 tcggcgcaat gggtgtgcca gcggacgcgc gcatcccggg ccaggcggac cgcacggacc
    11041 aggaggtcgg gctcgacgta cgtcggattg attggggcgg gccagacctc cacccggccg
    11101 cctgccggcc gggcatccag gcacgccgcg gtggcttcga gttcctcttc cgcgcttcgc
    11161 ccgaacagca ggtcggggat cccccggtcc ctggccacgt cggtccgggg gccgaagatg
    11221 ccccgggcga ccacgcctct gagcccgacc tcctccatcg cctccgcgac ggcaagggtg
    11281 gaggccgggt cggcgggggc gtagtggttg tcgagcaggc tcgtcgtgcc ggcccgcacg
    11341 gcctccaccg cggcgagccg ggcagccgcc cgggcatcct ccggcgtgac gtggtcggca
    11401 tatggccaga tcagccgggt gagccagtcc cacagcgcga gaccgtcgcc caacccccga
    11461 cagagcccct ggaacaggtg gttatgacag tccacgaggc cgggaaggac tgcgcatccc
    11521 gagcagtcga cgacccgccc cccggcggcg tctgggatct gcccaccggg cgccacggcg
    11581 acgatccgtc cgcccgccac cgcgacgtcg ccgcgatcga ggacggtccg gccggcgtcc
    11641 atcgtcacga ccaccccgcc ccgtagcacg aggtggtgac ggtggtctag gtccggcatc
    11701 tgtatatcat gtacagatgc ctggcccgga ctcaagcggc tcggcgatcg gcttgggcct
    11761 ccccagggcc gaggacgccg agcttctcaa tggctctgcc cggttcgtgg gggacctgct
    11821 ccccgacggc tgcctccacc tcgccttcgt ccggaaccgc gagccacacg cccggctgct
    11881 cggggtgtcg ctcgaacggg cccgcgcagc accgggcgtg gcgacggcct tggccgccgc
    11941 cgacctcggg cccagttcgt caatgccgtc ggcaccatgg ctcgggctcc cggaggcgtt
    12001 ctcccgtcct gtcctggccc gagaccgggt ccggtttgtc ggggaacccg tggcggttgt
    12061 ggtggccgag caccgagccc ggggcgagga tgccgccgag ctggcggagg tcgccctcga
    12121 gcctctgccc ccggtgctgg acccgaccga gggcgccgac tcggcctccc caaggctgtt
    12181 cgagtcggag agcaacgtcg tcctcgaaga gcgcgccggc cacgaggacg cgggagccgc
    12241 ggtcatgaac gcccccgtgg tcgttgaggg gaccttccgg atcgggcggc tcgcccacct
    12301 gtcgctcgag gcaagggccg tcctggccgt tcccgagccg cgcggaggcc tcacggtttg
    12361 gtgttcccac caggcccccc accggctgcg ctcaggactg gccgcggcgc tgggcctcga
    12421 tgccgggctg atccgggtgc gcgtccccgc ggtgggcggc gcgttcgggg ggaagagtca
    12481 gacttacccg gagtacgtcc tggcctgccg ccttgccctg gagctcggcc gcccggtccg
    12541 gtgggtggag gaccgccggg aggccttcct ggcggccgtg cacgggcggg ggtcgaacat
    12601 tcgcctgcgc gtcggcgctg ctcgcgacgg gaccctcctg gcgctggaga cggcgatcga
    12661 cggcgatctc ggtgcctacc cagagaccgg agcgctggtg ccgacgttcg cagcctggct
    12721 gatctcgggg ccctaccgga tcccccgcct ctcatccgtc gtccgcggcg tggttacgaa
    12781 caagccaccc accgcaccct atcggggggc cggccggccc gaggccgcgt acgtcctgga
    12841 gcggtcgatc gacctcctgg ccgaccgact ccggctggac ccgggcgagg tccgtcgccg
    12901 gaacctcatc cccgccgacg ccttccccta cgcgtcgccg actggcgccg tgtacgacag
    12961 tggcgactac cgccgggcac ttgaccgcgc cctcgagctg gctgcctacg aggagctccg
    13021 ccgcgacgag gcctggcgcc tcggcgacga tctgggcggg cgggttgtgc ggggggtggg
    13081 gatggccgtg tacgtcgagc gttccggagg aacgagcgac ctcgccgact acgcgcgggt
    13141 cgacctcgag ccggacggca cgctgaccat tcggagcgga gcctgctctc acggccaagg
    13201 acaccgcacc gccctcgccc aggtcgccgc tgcggcgctg gaggtcgacc tggaccgggt
    13261 ccgggtggtc caaggcgaca ccgccgaggt ttcgagcggg ggcggcacgt tcgggagccg
    13321 ctcaatgcag atcgggggcg cggccgtcca cctcgcatgc tgcgagttca tcgccctggc
    13381 gatccggcgg gctgcggagg ccttgggcac cgaccgggcg tccctccagt acgaaggagg
    13441 gatggtccgg tcggctggca cggagctccc gctggaggac ctcgcctgca ggaccggacc
    13501 tctgtctgcg gagtcacggt tcagcggaca gcaggcgttc ccgtttgggg cctatgtcgc
    13561 gacagtcgac atcgaccggg agaccggagc ggtgcgagtc acccggctcg tggcggtgga
    13621 cgactgcggg gtggtggtga acccgctgct gctcgaggga cagatccagg gctcgatcgt
    13681 gcagggcctc ggccaggctc tcaccgagga ggtagggtac gagccggacg ggcgacccac
    13741 gacggcgacg ctattctcct acgtcactcc aggcccggtg gagttgcccg acctggtgct
    13801 ggatcggacc gtaacaccga accccaacgt gccgacgggc gcgaagggag gaggcgaggc
    13861 cggtacgatc ggggcccctc cggcgatcgt taacgcgatc gtggacgccc tgcgcggata
    13921 cgacacgagc gtgatcacga tgccggtgac gcccgaggtg gtatggaagg ccctcagctc
    13981 gccccgtcgg gtgggatgaa ccggcccgat cctggttcgg cggttaccac ccggtcctcc
    14041 atcacgaccc ggccgccggc aacggtcatt accggcatgc cccggagcgg ccagccttcg
    14101 tacggggtgt tcccgcactt cgagtagagc ttcgtggagt cgacaatgcc gcgctcatcg
    14161 aggtccacta ggacgaggtc ggcgtcggag cccacccgga tcgtgccctt gcgtgggtac
    14221 acgtcgaggt gcttggccac gttcgttgag aagagctgaa ccaggcggtg caccgtgagc
    14281 ggggcgccct ccgcgatgcg ggtgaggaac agcgccagca tgtgctcgac gatcgtgagc
    14341 gagctcacta tcgagttgaa cgcgtccgcc cgactctcca gaagttcgtc ggtcgagtgg
    14401 ggcgcgtggt cgctcgccag gaggtcgatc gtcccatcgt tcagcgactc ccagatcggc
    14461 ccctcgtcga tcgtgtggtc cgggatgccc ttgccgaaga tcgcccatgg gttctgctcc
    14521 atcacggcct ggtaccccgc cgccttcatc ccccgaacga gcctgatcac cggcgggtag
    14581 ttgttgtgta gcactcggag ccggcatctg gtcacgtcgg ccagaagcat caccgtggcg
    14641 atggcggagt tctggacgaa gttccccgcg tacgtgtagg cggcctcccg gtgcttcggg
    14701 agggttgtgc ccgcctcgac gaattcccgg gtgagctcct gccacatgct ctggttgtga
    14761 gggtggacca ggcagggccg gccggtcgcc gcgatctcca ccatggtccg gtagatccgg
    14821 tgcggctcgg cgatcgagat gaactgctcc ggattgtcgt acgggtagtg gtggctgatc
    14881 atgaagaact tgaaccccat cgctccctcc cgggcgatcc cccggatctc ctcgggcaca
    14941 gtgggtgacg cccagtggtt gaagtcaacc agcgacttcg acgctgcgtt ggcccggtgg
    15001 gcccggaacc gctcgaccgt gttcggcacc gggtccgtgt tgggcatgtc gaccaccatc
    15061 gtcacgccgc cggccgcggc ggcccgggtc ccggtctcgt agtcctcctt gtaggtgaac
    15121 cctgggtcgc gaaggtggac gtgggtgtcg atcagcccgg ggaggagcag ccgaccctcg
    15181 gcgtcgacca cccgaccggc cgacgggaag tcccaggcgg ggacggcaag cgccacgacc
    15241 ttcccctccc ggatcccgag gtcgaggcga cgaaaggtgc cgtcctcccg ccacacggag
    15301 gctccacgga tcgccaggtc gaaactcata ctgcgcagat ccgccggact tcgggcagca
    15361 cgtcctcggc gatcaggtcg agcatctcct cgtactggtc gaaccgaagc cgcatgtcga
    15421 acacgaagtg ctgcacgccc gactcgacgt gcttcacgac ctcctcgacg agcacgtctg
    15481 gggggccggc caccagcgca ccgtcgagat cctcgaggga ctcgaacacg ccggactcac
    15541 ctggcggata tctcttcgcg gtctcctcga tgagggcgtc caggtgcagg cccgcggtgg
    15601 cctcctccat cgttcgtccc gggctcacgt aggggatcgt cccggtcgcc ggcacgcgac
    15661 ggccagcctc ctgcgccagc cgggtcatcc gctccacccg tttgcggaag tcccgccggg
    15721 gcatccgccc ggggacccac ccgtcgcaga actccaccgc gcgccgcacc gaggccgggc
    15781 tggaccctga gtaccaaacc gggacggcac cagaccgggg cttggggctg atggccacgt
    15841 ctgagaacga gtaaaactcg ccctggtggc tgacgccgtc ctcccgccag accttccgca
    15901 tgatttcgac ctgctcgggg aggaccttcc gccggtccca ggcccccatc cccacggcct
    15961 cgaactcgtg gtcgaacgac cccagcccga acccgaggat caggcggtcg cccccggtca
    16021 gctggtccag ggtgccgaag agcagggccg tgtggagagg atggcggtac gggatcaggg
    16081 ctccggtgcc cagggtcacc cggctggtca ccgcggcgat cgccgcgagg gccagggttg
    16141 gttcccagaa cgtggagtcg cccccttcat ggccgtgcgg ccggaagact acgtggtccc
    16201 ggacccagat cgagtcgaag ccgagccgct cgatctcggc cgaccgctcg atgatgcgat
    16261 cacggttcgc gtgcgggccg aagtgcggga gcaggatgcc gaatctcggg gttccgtcag
    16321 gcacgagcgc ctccaagtca gggaggggcg aatactatat ccagcatggg gcccccgccg
    16381 gatcggcctg gccgcgagcg ttcggcgggc cccgccgcga ccatcgtcga ggggctcaag
    16441 ggtgcgggcg tctccctcgc cgcttacgtg cccgactcgc tcctgcgctc taccttccgg
    16501 ctcctccgcg aggaccccga catccgcacg gtgcagacct cgaacgaggc cgacctgccg
    16561 ggaatcgtcg tgggggccta cctggggggt gtgcgggccg tattgatgat ggagaactcc
    16621 gggatacgac aggggtgtga accgatcgcc cgcttcacgt actcctaccg cttcccgatg
    16681 gtgatgctcc tgtcccacag gggcgggttc ccggagccga cctggtgggg ccacgcacac
    16741 gcccagacga tggcacccat cctcgacgcc ctccgggtcc cgtggcggct gctggagacc
    16801 ccggaggagg tcggccccgc cgtggccggg gcgttcaccc actcgcggtc cagccagtgg
    16861 ccagtggctc tcgtgctctc cggcgagtgc ctggagcgct gaggtgcagc ggcgggacgt
    16921 gctctccacg ctggcccggc tggtcacgcc cgaggacctg gtggtcacct ggcggagcac
    16981 cacctgggac gactgggcag agctcgcccc ggccggggcc cggaacacgc ttccgctggc
    17041 catcctgggg tcgatctcca ctaccgcgct cggcctggcc atcgcgctcc cctaccggcg
    17101 ggtcgtagcg gtggagtccg acggctcggt gctgatgaac gccggcgcga tggccacctt
    17161 gggcgcggag cggcccccga acctcaccgt ggtcgtcctc gacaacggga tgtacgagag
    17221 catcggcggg ccgcccaccc tcaccgcgcg ccacgccgac ctggccagga tggccgaggg
    17281 cgccgggtgt ccgaacagtc tcgccgtgga cgggcaggcg gccctcgagg aggccgcggc
    17341 caggctcctc gacgacggcg agatgggcta cctggtcgcc aggatcaagc ccgggcgtcc
    17401 ggcaggcacg ccacgcacgc ctatgacgtg gccccgcacc gacggcgtgg aggacaagta
    17461 ccgcttcctc cggtacgtcg aggaactcga gggcatcgtg atccaccctg acccgggccc
    17521 cggcccggga cgggtcggag gctagccctc cttcgcgacg tgcacgaagt cctggcccgg
    17581 cggcgtgtag atctccacga gtcgcgcctc cgtatccccg acgttcgacg cggagtgggg
    17641 cacgttcggc ggaatgaaca cggccattcc gggcttcatc tggtgggcga ccccgtcgat
    17701 cgtgacgacg acgtcgccct ccaagatgaa gtagatgttc tcgcagttgg tgtggaggtg
    17761 gtacgggccc ggcgctccac gcgccttcag cacgttgatg tgcatgtcga gccgctcggc
    17821 tccgaggttc gggtccacta gctggatcgc atacccgcgc tcgagctgca tgtctctacg
    17881 gggcgcggtg tccgggtcca cgaccttgcc ctcgctgggg gcctcctcgc tcatcctcgt
    17941 tcctcctctc cactccgtgg ccgtccgggc ttctcccgaa tctcggcctg ctcgccacct
    18001 cgcaggtacc ggtcgaacca gtcgaggcca tcgccttccc cctacctccg gggtcgaacg
    18061 agaggacggc gcatcccgcc tcgacgaagg gccgagccgc cgggctttgt ccgccatggt
    18121 cccgccgaac cccaggcaga cgacgatccc ggcgcattga gcaccgtgcg aagggacggg
    18181 gaacagcggc gcggcgaggg accagccgcc gctcacgaac aggacgtttg ctgggctcat
    18241 cgggtggcga cgtcagtccc catgtccgcg tcccggccgg gctgcagcga gcgcaggtac
    18301 gccgccacgt ccgcggccgg catgacgttg gcgtacttgg aatccatgtc gaacaggttc
    18361 acggcgtggg gcagctcagc acggtcgaag gtcccgtcct cgaccacggc gacccggaag
    18421 ccgtaggaga acgcgtcggt caccgacgct cgcacgcagc cggacgtcgt gcagcccgcc
    18481 acgatgacgg agtcgatgcc gaggtcgatc agggtcgccg tgaggggggt gccgaagaag
    18541 gcgctcggct tggtcttctc gatgaggacc tcccccggac ccggggcgat ctcctccacg
    18601 atcacctggg tatccggcgg acgctcgagc gtcctgcggt gcttgtcccg ccaccggccg
    18661 aggttctcct cgacgtgacg cgacatcccc gctgtgtaga agacggggat cgaggcggcc
    18721 cgtgcggcgt cgaggacctc ccggatgcgg ggaaccgcct tccacccgga aggtccgcag
    18781 gcgttgggat gcacgcggat cgcgtcccgg agcgagaggc ccggcttgcc gacgaagccg
    18841 taggtgacgt cgatcacgag gcatgccgga cggcggccga gctccatccg gcccccgtac
    18901 ccggccaccc cgtagacctc gaggtcggcc ggggggatca gctcccgcca tcccatgcgc
    18961 ctagacctcg ctccacggca tcaacgcgcc ccggaggcga cggatgccgg atcggaaggc
    19021 cacgctcagc gcgacgatcg ccagcagcgc gacgaacatg cggtcggtgc ggaagaagtt
    19081 ggagtactcg acgatgagac cccccaggcc ccgaatgacc agaagcatct ccccgagaac
    19141 catcccgatg agtgccgttc cgctcgcgac gttaagcccc gcgaagaagt acggcaggat
    19201 cgccggcacc acgatgctcc gcagggtctg acgattggtg gctccgtagt tgcgggcgac
    19261 gtcgatgagg tcctggctga caagcttcgc gccggccatc gtgttgagca gcaccggcac
    19321 caggctcgtg ctgaagacga tcgccacccg ggtcgtggac ccgatgccga tccagatgat
    19381 caagagcggc ataatcacca cggtcggaag cgcatacagg aaggacacgt aggggtccag
    19441 cacgtactcc aacagcctga accggcccat caacaccccg aagagagtgc ccaccacgat
    19501 ggcggcggag aagccgataa cgagcaccgt gaggctgtcg gcgagcgccg tccatagcac
    19561 gcggctgtcg atggccagct ccttgaaggc gagcacgacc t
//
LOCUS       DASUTW010000270         6803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_736804, whole genome shotgun sequence.
ACCESSION   DASUTW010000270 DASUTW010000000
VERSION     DASUTW010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736804"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2351
                     /locus_tag="VF058_09100"
     CDS             <1..2351
                     /locus_tag="VF058_09100"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX6208497.1"
                     /translation="PWTPLDSVTIGKAIAFSLSFDVDIELTLDYLQYVGTGQALGFDG
                     HALFTQDAFRSQPFSDASTVPDATGAPLATVDRHTARAASKAAARLDPDTVRLARGWL
                     RRIERVPFFDPVLDEDLGAGSNEWGVAGSLTDSGFPMIANDPHLALDMPSTFYPIHLR
                     AGSMDVYGEGFAGAPGVILGRNRFISWGATTNPMDVTDTFQEQIVPDASSPSGLSTVF
                     QGQREPVIPIPEQYRANVGGNVVPVPPSPSIPPATLIVPRRNQGPIVALDAAAGTALS
                     VQYTGFSGTRELETFLIWDRARNLEDFRRGLEAFDFGSQNWAYADVRGNLAHFTSAEM
                     PLREDLEAGSVNGLPPWFIRNGTGGNEWLSRTTTHPGQAIPYEILPPEEMLQVVNPSA
                     GFFVNANNDPAGTTLDNDPLNQLRPSGGIFYLNPGYDGFRAGRITEMLRERMGVGERI
                     SFEDVQEMQSDVALIDAEVLVPHVLGAWERAQGSATSELADLAADAGVAEAVARLEAW
                     DFTTPTGIPEGYDAADEAGRLSPPSPEEIDHSVAATVYALWRSHVLANVIDGTLQGLG
                     LDPPGSGQAMASLRHHLEAFGSAQGVGASGIDFFRVPGVADPGDERDVLLLRSVREAL
                     DTLASPGMAAAFGGSSDQDDYRWGRLHRIVFDHPLGGPFSLPPAGGAFPQPLPGLPGI
                     PTDGGFGVVDASSHNPRARTVNGFMFGGGPVRRFVGEMAPGLARTRSESSLPGGTSGL
                     LGDPRSFNLLPDWLTNEAYPQLLRESDLRATLVSVQRLVPAS"
     gene            complement(2355..3551)
                     /locus_tag="VF058_09105"
     CDS             complement(2355..3551)
                     /locus_tag="VF058_09105"
                     /inference="COORDINATES: protein motif:HMM:NF014237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX6208498.1"
                     /translation="MIATLVAAVLGTLISTSPASAAGCLRWGALPPSSPGDVSELEAL
                     EGAVGRPVDYLGYYQGWAARDAFDTTRAGAAVARGATPVVAWEPWDWTGGVNQPEFRL
                     ARVIAGDHDDYIRAWAQDAKAFGGRIILRFAYEMNGDWMPWSESVNGNRPGEYVRAWR
                     HVHRIFDQVGARNVRWIWAPNVVSPAFTPLKRLYPGDRWVDLVGIDGYNWGTTQSSWG
                     STWQSFDRIFRPTIRKLRRITSKRILITEIASTEHGGDKAAWTRKTLRAMGRYPFIVG
                     FMWFNYDKETDWRIQSSASSLRSFRKALSHHRFSCRLRLKARSRSVRRGAPAKLVVRV
                     RPYGGERAHVVLSRGGKKVRARWTGPSPSKLVFEVPARRAARYVARLRGPQPRMRSNP
                     VRVRVR"
     gene            complement(3728..5080)
                     /locus_tag="VF058_09110"
     CDS             complement(3728..5080)
                     /locus_tag="VF058_09110"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6208499.1"
                     /translation="MDRLDFSLGRLLVLSVLIGAIWLSINAPLDGALNWYLTFAWSFF
                     LPLVLIGLLGGARVRDRLTSRYEGQVSDLVIFLIPTVARRDVLPALHRVVDSVLLYAP
                     ANLRRFRVDLVVDEGSDGIPELEAVYGDHPLVRILVVPKEYETPGGAIHKARATHYAL
                     LVRDAEGESGPDVFVYHLDDDTGVGGDTISSIAEFIADDDGTYDLAQGVLAFPHHLAS
                     STFCAYADSIRPGDDVTRFRFFTGVLGKPLGGLHGEHLLVRASVESEIGWDFGGTKVE
                     DAHFAMRFAARGHRSKALDSCSYGASPSSLRDLITQRRRWASGLIQLIFDRSIPLRTK
                     PFIAYSVLLWITGFFYHPVLMLALASAVGPGANTSPVLAVVTPIWAVNLAFILTQYLE
                     GLRVNLATTQGRPRRLRAALLMIPGFYVFTAVEAYAAALGLKDAILGRRDFQVIRKPV
                     "
     gene            5621..6637
                     /locus_tag="VF058_09115"
     CDS             5621..6637
                     /locus_tag="VF058_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208500.1"
                     /translation="MGADTPSPTPSFVEVNEPRTSEEDFQAGVAVLAYGDEEPDAFRE
                     KSAAALDRLAELHVNSVSFVFPVVQGDWRATEIGPDPERTPSDEAIRAFIEEAERRGF
                     TVLLRPVLDEESLTPDGKWRGSIEPSDLDAWFASYGRLMKRYAQLSAEAGADILAVGT
                     ELTSLQDEAERWRRLIREVRRVFDGQITYATNWDAIGPAYAPTFVGALDFLSVDAFFP
                     LEVGEAAAAGEMARGWRPWLRELRRMGPALRNVVFTEVGVRAQRGAHLKPYVWEHGGE
                     TDPETQRRYYAATCRAVGEEIGGLYWWVVDIHHAVEAEAVEDFSPMGKPAEEEMARCF
                     RSVA"
BASE COUNT         1038 a         2341 c         2454 g          970 t
ORIGIN      
        1 agccgtggac gccgctggac agcgtgacga tcgggaaggc gatcgcgttc tcgctgtcgt
       61 tcgacgtgga catcgagctc acgctggact acctgcagta cgtggggacc gggcaggcgc
      121 tggggttcga cgggcacgcg ctcttcaccc aggacgcttt ccggtcgcag cccttctccg
      181 acgcctcgac ggtgccggac gccaccggtg ccccgctcgc gacggtcgat cgtcacacgg
      241 cgcgcgccgc gtcgaaggcg gcggcgcgcc tcgacccgga cacggtccgt ctcgcgcgcg
      301 gctggctccg tcggatcgag cgcgtcccgt tcttcgaccc ggtcctggac gaagacctcg
      361 gggcgggctc gaacgagtgg ggtgttgcgg ggtccctcac cgactcgggc ttcccgatga
      421 tcgccaacga cccgcacctg gccctcgaca tgccctcgac gttctatccg atccacctcc
      481 gggccggctc gatggacgtc tacggcgagg gcttcgcggg cgcgccgggg gtcatcctgg
      541 ggcggaaccg cttcatctcg tggggcgcca ccacgaaccc gatggacgtg accgacacgt
      601 tccaggagca gatcgtcccc gacgcgagct ccccgagcgg gctgagcacc gtgttccagg
      661 gccagcggga gccggtcatc ccgatccccg agcagtaccg cgcgaacgtg ggcgggaacg
      721 tggttcccgt gccgccgagc ccgagcatcc ctccggccac cctgatcgta ccccgccgga
      781 accagggacc gatcgtcgct ctcgatgccg cggcggggac ggcgctctcg gtgcagtaca
      841 cgggcttcag cgggacgcgg gagctggaga cattcctgat ctgggaccgc gcccggaacc
      901 tcgaggactt ccgccggggc ctcgaggcct tcgacttcgg gtcccagaac tgggcctacg
      961 ccgacgtccg ggggaacctg gcccacttca ccagcgccga gatgccgctc cgcgaggacc
     1021 tcgaggcggg ctcggtgaac gggctgccgc cgtggttcat ccggaacggc accgggggca
     1081 acgagtggct gtcgcggacc acgacgcacc cgggccaggc gatcccgtac gagatcctcc
     1141 cgcccgagga gatgctgcag gtcgtgaacc cctcggccgg tttcttcgtg aacgccaaca
     1201 acgacccggc cggcaccacg ctcgacaacg accccctgaa tcagctgcgg ccgagtgggg
     1261 ggatcttcta tctgaacccg ggctacgacg ggttccgtgc cgggcggatc acggagatgc
     1321 tccgcgagcg gatgggggtc ggcgagcgaa tctcgttcga ggacgtgcag gagatgcagt
     1381 cggacgtggc gctgatcgac gcggaggtcc tcgtgcctca cgtcctcggg gcgtgggagc
     1441 gcgctcaggg gagcgccacg tcggagctcg ccgatctggc tgcggatgcg ggcgtcgccg
     1501 aggccgtggc gcgcctggag gcctgggact tcacgacgcc gaccggcatc cccgagggct
     1561 acgacgccgc ggatgaggcc ggccggctct cgccgccgtc gccggaggag atcgaccaca
     1621 gcgtggcggc cacggtctac gcgctctggc ggagccatgt cctggcgaac gtgatcgacg
     1681 ggaccctgca gggcctgggt ctcgacccgc ccgggagcgg ccaggcgatg gcgtcgctcc
     1741 gccatcacct cgaggccttc gggtcggcgc agggcgtcgg ggcctccggc atcgacttct
     1801 tccgggtccc tggggtggcc gatcccgggg acgaacgaga cgtgctcctc ctgcgcagcg
     1861 tccgagaggc gctcgacacc ttggcctccc cgggcatggc cgccgcgttc ggcggctcga
     1921 gcgatcagga cgactaccgg tgggggaggc tgcaccggat cgtcttcgat catccgctgg
     1981 gcggcccctt cagcctgccg cccgccggcg gggccttccc ccagccactg cccggcctcc
     2041 cggggatccc caccgacggg gggttcggcg tggtggacgc gtccagccac aacccgaggg
     2101 cgaggaccgt gaacggcttc atgttcgggg gagggccggt gcggcgcttc gtgggcgaga
     2161 tggctcccgg gctggccagg acccggtccg agtcgagcct gcccggaggg acgagcgggc
     2221 tgctcggcga tcctcgctcc ttcaacctgc tgccggactg gctgacgaac gaggcgtacc
     2281 cacagctcct ccgagagagc gacctccggg ccacgctcgt ctcggtgcag cggctcgttc
     2341 cggcctcctg agggtcagcg gacccgaaca cgcaccgggt tcgagcgcat ccgtggctgg
     2401 ggaccgcgga ggcgagccac gtagcgtgcg gccctccgag ccggaacctc gaaaacgagc
     2461 ttcgaaggac tggggccggt ccatcgcgcc cggaccttct tccctccccg ggagagcacg
     2521 acgtgggctc gctcgcctcc gtacggcctc acgcggacga cgagcttcgc gggcgcgccc
     2581 cgtcggacgc tccgcgacct cgccttcagc cgcagccggc agctgaagcg atggtgcgag
     2641 agggccttcc ggaaggagcg gagggagctg gccgagctct ggatccgcca gtcggtctcc
     2701 ttgtcgtagt tgaaccacat gaagccgacg atgaaggggt accgacccat cgccctgagc
     2761 gtcttccgtg tccaggccgc cttgtcgccg ccgtgctccg tgctcgcgat ctcggtgatg
     2821 aggatgcgct tggatgtgat gcgacggagc ttcctgatcg tcgggcggaa gatccggtcg
     2881 aaggactgcc aggtgctgcc ccatgaggac tgggtggtcc cccagttgta accgtcgatc
     2941 cccacgaggt ctacccagcg atcgccgggg tagaggcgct tcagcggcgt gaaggcgggc
     3001 gagacgacat tgggcgccca gatccacctc acgttgcgcg cgccgacctg gtcgaagatg
     3061 cggtggacgt gcctccaggc ccgcacgtac tcgccgggcc ggttgccgtt gacgctctcg
     3121 gaccagggca tccagtcccc gttcatctcg taggcgaacc gcaggatgat gcgccccccg
     3181 aacgctttcg cgtcctgggc ccaggcccgg atgtagtcgt cgtggtcgcc ggcgatgacg
     3241 cgggccagtc ggaactccgg ctggttgacc ccgccggtcc agtcccaagg ctcccaggcc
     3301 acgacgggag tggcgccccg ggcgacggcc gcgccggccc gcgtcgtgtc gaaagcgtcg
     3361 cgcgccgccc agccctggta gtagccgagg tagtccaccg gtcgtcccac ggcgccctcc
     3421 agggcctcga gctcgctcac gtcaccggga gaggagggag ggagagcgcc ccaccgaagg
     3481 cagccggccg cggaggcggg ggaggtcgag atcagggtcc ccaggacggc cgcgaccagg
     3541 gtcgcgatca ccgctctcgt cctgcgcgga tgcctccctc tcatgccccc cgcatcggca
     3601 cggtccggag acgcgttgag cagggcgacc aggcggagcc gatggggagg tcgggcagct
     3661 ggcgggaacc cggcacccgc acagggtgcc gggcctcacg cccgatttca actctttcgc
     3721 ttggatctca cacgggcttg cggatcacct ggaagtcccg gcgccctagg atcgcgtcct
     3781 tcagcccgag agccgcagcg taggcctcca cggccgtgaa gacgtagaac cccgggatca
     3841 tgaggagggc agccctcaac cggcggggcc ggccctgcgt cgtggccagg ttcacccgca
     3901 gcccctcgag gtactgggtc aggatgaacg cgaggttcac cgcccagatc ggggtgacca
     3961 ccgccaggac cggggaggtg ttcgccccgg gccccacggc ggaggcgagc gccagcatca
     4021 ggacggggtg gtagaagaag ccggtgatcc acagcaggac cgagtaggcg atgaacggct
     4081 tcgtccggag cgggatcgag cggtcgaaga tgagctggat cagcccgctg gcccacctcc
     4141 gtcgctgagt gatgaggtcc cggaggctcg agggcgacgc tccgtaggaa caggagtcga
     4201 gcgccttaga gcggtggccg cgcgccgcga accgcatcgc gaagtgggcg tcctcgacct
     4261 tcgtgccgcc gaagtcccag ccgatctcgg attcgacgct ggcccggacc agcaggtgct
     4321 cgccgtgcag gccccccagc ggcttcccca gcacgcctgt gaagaaccgg aacctcgtga
     4381 cgtcgtcgcc agggcggatc gagtccgcgt aggcgcagaa cgtcgacgag gcgaggtggt
     4441 gggggaaggc cagcaccccc tgcgccaggt cgtaggtgcc gtcgtcgtcg gcgatgaact
     4501 cggcgatcga ggagatcgtg tcgcccccaa cgccggtgtc gtcatcgagg tggtagacga
     4561 agacgtccgg gccgctctct ccctccgcgt cccgcaccag gagcgcgtag tgggtggcgc
     4621 gggccttgtg gatcgccccg ccgggcgtct cgtactcctt cgggacgacg aggatccgaa
     4681 cgagcgggtg gtcgccgtag acagcctcga gctcggggat gccgtcggaa ccctcgtcga
     4741 ccaccaggtc cacgcggaac cggcggaggt tggccggcgc gtagagcagc accgagtcca
     4801 ccacccggtg cagcgcgggc agcacgtcac gccgggccac ggtcgggatc aggaagatca
     4861 cgaggtcgga gacctgcccc tcgtacctcg aggtcagtct gtctcgcaca cgggctcccc
     4921 ccagcagccc gatgaggacc agggggagaa agaacgacca ggcgaacgtc aggtaccagt
     4981 tgagcgctcc atcgagcggc gcgttgatgg agagccagat cgccccgatc aggaccgaga
     5041 gcaccagcag ccgcccgagg gagaagtcga gccgatccat gatcggcgtg tggctgatcc
     5101 tggggagagg tcgggcgatg cgatggggct ccagctccct gggccgcagg tccacccggg
     5161 ggaaggggaa ccgccggagg acacgcggtc gtggcgcggt ctggggccgc aggtccaccc
     5221 gagggaaggg gaaccggcgg aggacacgcg gccggccccg gatgtgccgg gctcagctcc
     5281 tcctcatgtg cacggttcgg ttgggtcgcc atttgtcacc tcgcatgtca gtcaacggta
     5341 tcgagcaggg tgaactctcg ttacccagcg agtggaagaa cgcaaacaca aaaaacgaaa
     5401 aggtgaagtt cgccaacccc cggacgcatg accgtccccg ggtggggaag agttcctggg
     5461 tcgtctaccc cacacgagag gtgaagaacc catcgagccc acgttccggc gccccgcgct
     5521 tctgatcggc tcggtcgtcg ccttcgtcgt cgcgacgtcg tgcggcggcg agccgctcct
     5581 ggacctcgat cccgagcccg agtccccgag cagcccgacg gtgggcgcgg acacgccgtc
     5641 gccgacgccc tcgttcgtgg aggtgaacga gccccgcacc tccgaggagg acttccaggc
     5701 gggcgtcgcg gtcctcgcct atggcgacga ggagcccgac gcgttccggg agaagagcgc
     5761 cgccgcgctc gaccgcctcg cggagctcca cgtcaactcg gttagcttcg tcttccccgt
     5821 ggtccaaggc gactggcggg ccaccgagat cggacccgac ccggaacgga cgccctctga
     5881 cgaggcgatc cgggccttca tcgaggaggc cgagcgtcga ggattcaccg tgctgctccg
     5941 accggtcctc gacgaggaga gcctcacacc ggacgggaag tggcgcggca gcatcgaacc
     6001 gagcgacctc gacgcctggt tcgcctccta cggccggctg atgaagcggt acgcgcagct
     6061 ctccgcggag gccggggccg acatcctcgc ggtggggacc gagctgacga gcctccaaga
     6121 cgaggccgag cgctggcgac ggctcatccg ggaggtccgc agggtgttcg acgggcagat
     6181 cacgtacgcc acgaactggg acgcgatcgg ccccgcctac gccccgacct tcgtgggcgc
     6241 cctggacttc ctctccgtgg acgcgttctt cccgctggag gtgggcgagg ccgccgccgc
     6301 tggggagatg gcgcgggggt ggcggccctg gctccgggag ctgcggcgga tggggccagc
     6361 gctccggaac gtcgtgttca ccgaggtcgg ggtgcgggca cagcgcgggg cccacctcaa
     6421 gccgtacgtc tgggagcacg ggggggagac ggacccggag acccagcgcc ggtactacgc
     6481 ggccacgtgc agggcggtgg gcgaggagat cggcgggctc tactggtggg tggtggacat
     6541 ccatcacgcc gtcgaggctg aggccgtcga ggacttcagc ccgatgggga agccggccga
     6601 ggaggagatg gcccgatgtt tccggagcgt cgcctgaggc ccggcgtctt gtctggctcg
     6661 ggccgcggcg ttagagtgcc gggcacgctt ccgggcaggg gggttcacat gcgaccgtcg
     6721 tcacgctcaa cgctccgcgt cctcgccgcc accgccgtcg cggtcctcgc gctcgcgtcg
     6781 ggcccggtgg cccaggccga ccc
//
LOCUS       DASUTW010000271         4959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_608093, whole genome shotgun sequence.
ACCESSION   DASUTW010000271 DASUTW010000000
VERSION     DASUTW010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608093"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..115
                     /locus_tag="VF058_09120"
     tRNA            44..115
                     /locus_tag="VF058_09120"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:76..78,aa:Ala,seq:cgc)
     gene            171..419
                     /locus_tag="VF058_09125"
     CDS             171..419
                     /locus_tag="VF058_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208501.1"
                     /translation="MAKVLVSIDDRLLARIDRAARESGLSRSGYLSRLAAREVESAPG
                     PGRDRKVHRALGRLDELFREHPVPEDATAAVRAERNAR"
     gene            416..823
                     /locus_tag="VF058_09130"
     CDS             416..823
                     /locus_tag="VF058_09130"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX6208502.1"
                     /translation="MTDLVLDASVLLKWFAPAPERGRKEAFDLRKEFEEGRLSVIVPS
                     LIFLEVLNVAGRRWGWESDALADLASALDELGFDVREPELPSVAAWVAQGLTAYDAAY
                     VALAERSRVDLVSDDDEVLGVAGELARPLIRPA"
     gene            complement(893..1294)
                     /locus_tag="VF058_09135"
     CDS             complement(893..1294)
                     /locus_tag="VF058_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6208503.1"
                     /translation="MGGFAGEPSALTTILVVSDVERSKAWYLDVLGADLYGEYGGTSV
                     VLSFLGAWLLLVTGGGPTEDKPTVTFVEQADPDRVDHAFTIRVADCHAAYEDLRGRGA
                     EFLTPPIEHGRETRAFFRDPDGHLFEISAVT"
     gene            1350..1697
                     /locus_tag="VF058_09140"
     CDS             1350..1697
                     /locus_tag="VF058_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6208504.1"
                     /translation="MVVDELTDEAVDEVFRALADPTRRDILRRSGLDAPSVSRLAEAY
                     PMSFAAVQKHVAVLERAGLVAKERRGREHLVRTVPEAVRLARRALDRLEATWRERVER
                     MSDLLDEEKGRER"
     gene            1694..2179
                     /locus_tag="VF058_09145"
     CDS             1694..2179
                     /locus_tag="VF058_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016824173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX6208505.1"
                     /translation="MSVTSIDKDLDGLTFTLIADFDAPVERVWELWADPRKLERWWGP
                     PGYPATFEKHDLAPGGEVAYYMTSPEGERNHGWWRFVSVDPPKALEIQMGFADEEGNP
                     IADMSASAVRVQLSERGGGTRMELRFSFDSPEEMERVMEMGMAEGLQQSVGQMDALLA
                     G"
     gene            2200..2811
                     /locus_tag="VF058_09150"
     CDS             2200..2811
                     /locus_tag="VF058_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6208506.1"
                     /translation="MRELIVQTFLTLDGVMQAPGGPEEDPSGGFEHGGWSVTYWDEEM
                     GRVMDEVTSTPFAMVLGRRTYDIMAAHWPHASEEEGAKVFNEATKYVASRGRPTLEWS
                     NSVLIEGDAAEGIAALKGEDGPELQVHGSANLIQGLLPTGLVDRFRLWTFPVVLGSGK
                     RLFGDGTIPSGLRLIDQGVSSTGVVMATYEPAGEVVTGSFALE"
     gene            2866..>4959
                     /locus_tag="VF058_09155"
     CDS             2866..>4959
                     /locus_tag="VF058_09155"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6208507.1"
                     /translation="MRELPTGTVTFLFTDIEGSTRLLQELGDGYATVQGEHDAILAQA
                     FQASGGVEVRREGDAFFVVFQKPIAALEAAVAAQRSLAEYDWPHGSPLRVRMGLHTGE
                     GALGGADYVGIDVNRAARIAAAAHGGQILLSEATRGLVEHALPGGVSLRDLGEHRLKD
                     IAHPERLYDVAIDGLPSDFPPLRTLEARPHNLPVQLTSFIGREREIEEATRLLQRARL
                     LTFTGPGGSGKTRLALQVAAETISDHEDGVFFVDLSAVTDPALVPSAIVGVLGVPEEP
                     GRPLSESLEDHLRGLDLLLILDNFEQVVEAAPIVEQLLTAAPRLRVIVTSRTPLGVRG
                     EHERPVPPFEPPDPGHLPDLPALCETEAVRLFTERALAVNPRFEVSEENAEEVAEVLV
                     RLDGLPLAIELAATRTKLLAPDQMLERLDHRLSLLEAGGRTLPERQRTLRGAIAWSYD
                     LLEESERTMFARLSIFAGGWTLASAEAVCDTLGLGLDPLDAMTSLVDKSLIRLAEASG
                     GDPRFSMLETIREFGREQLEALGELDDARRRHAEHFLDLALEVEPHLMDEDQSGWLDR
                     CDREHANIRRALRWAIEQGELARAQEAAGALWRFWHLRGHLAEGRRWFEELLAHPDGQ
                     GRTAARAKALTGAGGIAWWQADVPAARGFYEESLAIERGRGSPVRIAVAAYDMAHAAI
                     AGDEFEDALRLLEEAL"
BASE COUNT          734 a         1667 c         1828 g          730 t
ORIGIN      
        1 ggacctgcac ggctgacccg gggtgctacc atcgacccgt cccgggggtg tagctcaggg
       61 gtagagcgct gcgttcgcaa cgcagaggtc aggggttcaa atcccctcac ctccacatcg
      121 agccgctgtc tgcgctggac agaaggcgga tatccggata tcctcgggtc atggccaagg
      181 tgctcgtctc gatcgatgac cgtctcctgg cccgtatcga ccgcgcagcc agggagtcgg
      241 gtctgagccg cagcggctac ctcagccgcc tcgcggccag ggaggtggag tcggcccccg
      301 gtcccggccg cgatcggaag gtgcaccggg ctctcggccg cctagacgag ctcttccgcg
      361 agcaccccgt cccggaggat gcaacggcgg ccgtcagggc cgagcggaac gctcggtgac
      421 ggacctcgtt ctcgacgcgt cggttctgtt gaagtggttt gcgcccgcac ccgagagggg
      481 gcggaaggaa gcgttcgatc tccgcaagga gttcgaggag ggtcgtctct cggtgatcgt
      541 tccctcactc atctttctcg aggtgctgaa cgtggctggg aggaggtggg gctgggagag
      601 cgacgcgctc gccgacctcg cttcggccct ggacgagctc ggcttcgacg tcagggagcc
      661 ggagctcccc tcggtcgcgg cctgggtcgc gcaagggctg acggcgtacg atgccgcgta
      721 cgtcgcgctc gcggagcgaa gccgggtcga tctcgtctcc gacgacgatg aggtcctcgg
      781 ggtggccggc gagctcgctc gtccgctcat tcggccggct tgaccgggga gcgcatcgag
      841 gcggcacgac acgacccgat cgtgtcccgg ccgactcgtt cacgggcgag tctcacgtga
      901 ccgcgctgat ctcgaacagg tgtccgtccg ggtctcggaa gaaggccctc gtctcccgcc
      961 cgtgctcgat cggcggcgtg aggaactcgg cgccgcggcc ccgcaggtcc tcataggctg
     1021 cgtggcagtc ggcgacccgg atcgtgaatg cgtgatccac gcggtcgggg tccgcctgct
     1081 cgacgaaggt cacggtcggc ttgtcctcgg tcggcccgcc gccggtcacg agcaggagcc
     1141 aggcgccgag gaacgagagc accaccgacg tacctccgta ctcgccgtac agatcggccc
     1201 ccagcacgtc gaggtaccag gccttcgacc gctcgacgtc ggagacgacc aggatcgtcg
     1261 tcagcgcgct gggctccccc gcgaagcctc ccacgccgcg catcgtacag gcttgtcgga
     1321 tcggcgcccc cgcggtaacc tacaaccgta tggttgtaga tgagctgacc gacgaggccg
     1381 tggacgaggt gttccgggcc ctggccgacc ccacgcggcg cgacatcctc cgtcgctccg
     1441 ggctggacgc tccatccgtc tcacggctgg ccgaggccta ccccatgagc ttcgccgccg
     1501 tgcagaagca cgtcgcggtg ctggagcggg ccggcctcgt cgcgaaggag cgtcgtgggc
     1561 gcgagcacct cgtgcgcacc gtgcccgagg ccgtgcgcct ggcccgccgc gccctcgatc
     1621 ggctcgaggc gacgtggcgg gagcgggtag agaggatgtc ggacctgctc gacgaggaga
     1681 aaggacgaga gcgatgagcg tcaccagcat cgacaaggac cttgacggcc tgaccttcac
     1741 cctgatcgcg gacttcgacg cgccggtgga gcgggtgtgg gagctgtggg ccgaccctcg
     1801 gaagctggag cggtggtggg ggccgccggg gtatccggcg accttcgaga agcacgacct
     1861 ggcccccggc ggcgaggtcg cgtactacat gacgagcccg gagggagagc ggaaccacgg
     1921 ctggtggcgc ttcgtctccg tcgacccgcc gaaggcgctg gagatccaga tgggcttcgc
     1981 cgacgaggag gggaacccga tcgcggacat gtccgccagc gcggtgcggg tccagctgtc
     2041 cgagcgcggg ggaggcacga ggatggagct gcggttcagc ttcgactctc ctgaggagat
     2101 ggagcgggtc atggagatgg gcatggccga gggtctccag cagtcggtcg gccagatgga
     2161 cgccctgctg gcgggctgac cacccggaag gacgcgctga tgagagagct gatcgtacag
     2221 acgttcctga cgctggacgg ggtcatgcag gccccgggag gccccgagga ggaccccagc
     2281 ggcggcttcg agcacggcgg atggtccgtg acctactggg acgaggagat gggccgggtc
     2341 atggacgagg tgacgagcac gcccttcgcc atggtcctgg ggcgcaggac ctacgacatc
     2401 atggccgcgc actggccgca cgcgtccgag gaggagggcg cgaaggtctt caacgaggcc
     2461 acgaagtacg tggcctcgcg cggacggccc accctggagt ggagcaactc cgtgctgatc
     2521 gagggggacg ccgccgaagg catcgcggcc ctgaaggggg aggacgggcc ggagctccag
     2581 gtgcacggca gcgcgaacct gatccagggc ctgctgccga ccgggctcgt ggaccgcttc
     2641 cggctgtgga ccttccccgt ggtcctcggg tcggggaagc gcctgttcgg cgatgggacc
     2701 atcccctccg ggctgcggct catcgatcag ggagtctcct ccacgggggt ggtgatggcc
     2761 acgtacgagc ccgcgggcga agtcgtcacg ggatcgttcg ctctggagtg acctcgcgcg
     2821 ccgccccggg tcccgaatcc gcgaacgcgc tacatttcgt ccgccatgag ggagctcccg
     2881 accgggacgg tcaccttcct gttcaccgac atcgagggct cgacccggct gctccaggag
     2941 ctcggcgacg gctacgcgac cgtccaggga gagcacgacg ccatcctcgc ccaggcgttc
     3001 caggcctcgg gcggggtcga ggtccgccgc gaaggagacg cattcttcgt ggtgttccag
     3061 aagccgatcg ccgcgctcga ggccgccgtc gccgcccagc gaagcctcgc cgagtacgac
     3121 tggccccacg gttcgcccct ccgggtccgg atggggctcc acaccggcga gggggcgctg
     3181 ggcggcgccg actacgtggg catcgacgtg aaccgggccg cgcggatcgc ggccgcggcg
     3241 cacggcggtc agatcctgct ctcggaggcg acgcggggcc tcgtggagca cgcactcccg
     3301 ggcggcgtct cgctgcgcga cctgggggag catcgcctca aggacatcgc ccatcccgag
     3361 cgcctctacg acgtggcgat cgacggcctg ccctcggact tccctccgct ccggacgctc
     3421 gaggctcgcc cgcacaacct cccggtccag ctcacgtcct tcatcggtag ggagcgtgag
     3481 atcgaggaag cgacccgtct cctgcagcgc gcgcggctgc tgacgttcac cggcccgggg
     3541 ggcagcggga agacccgact ggccctccag gtggcggccg agacgatctc ggaccacgag
     3601 gacggagtgt tcttcgtcga cctctcggcg gtcacggatc cggcactcgt cccctccgcg
     3661 atcgtcggcg tcctgggcgt ccccgaggag ccgggtcgcc cgttgagcga gagcctggag
     3721 gaccatctcc gcggcctcga tctcctgctg atcctcgaca acttcgagca ggtcgtggag
     3781 gcggctccca tcgtcgagca gctcctcacg gcagctccga ggctcagggt gatcgtcacc
     3841 tcccgcacgc cgctcggggt gcgcggcgag cacgagcggc ccgtgccgcc gttcgagccg
     3901 cccgaccccg ggcacctccc ggatctccca gcgctctgcg agaccgaggc cgtgcggctt
     3961 ttcaccgagc gagcgttggc cgtgaacccg cgcttcgagg tcagcgagga gaacgccgag
     4021 gaggtggcgg aggtcctggt ccgcctcgac ggcctcccgc tggctatcga gctcgcggcg
     4081 acccggacga agctcctcgc cccggaccag atgctcgagc gactggacca tcgcctctcg
     4141 ctgttggagg cgggcggacg gacccttccc gagcgccagc gcaccctccg aggcgcgatc
     4201 gcctggagct acgacctgct ggaggagtcc gagcggacga tgttcgctcg tctgtcgatc
     4261 ttcgccggcg gctggaccct cgcctccgcg gaggcggtgt gcgacacgct ggggctcggc
     4321 ctggatcccc tggatgcgat gacctcgctc gtggacaagt cgctgatccg gctggccgag
     4381 gcctcgggag gcgatccacg gttctcgatg ctggagacga tccgggagtt cggccgagag
     4441 cagctcgagg cgctgggaga gctcgacgac gcccgccggc gccacgccga gcacttcctg
     4501 gatctggcgc tggaggtcga gccacacctc atggacgagg accagagcgg atggctcgat
     4561 cgctgcgacc gggagcacgc caacatccgg agggccctgc ggtgggcgat cgagcagggc
     4621 gagctcgctc gggctcagga ggcggccgga gcgctgtgga ggttctggca cctgcggggg
     4681 catctggccg aggggaggag gtggttcgag gagctcctgg cgcacccgga cgggcagggc
     4741 cggacggcgg ccagggcgaa ggccctcacg ggggcgggcg ggatcgcgtg gtggcaggcg
     4801 gacgtgcccg cggcgcgggg gttctacgaa gagtccctcg cgatcgagcg agggcggggg
     4861 agcccggttc ggatcgcggt ggccgcgtac gacatggcgc acgcggccat cgccggtgac
     4921 gagttcgagg atgctctgcg gctgctggag gaagcgctc
//
LOCUS       DASUTW010000272         7682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_348613, whole genome shotgun sequence.
ACCESSION   DASUTW010000272 DASUTW010000000
VERSION     DASUTW010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7682
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348613"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..3139)
                     /locus_tag="VF058_09160"
     CDS             complement(20..3139)
                     /locus_tag="VF058_09160"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6208508.1"
                     /translation="MSQQACRSCGELLPADARFCPSCGAAVAAAEGEERKLVTVLFAD
                     VIGSTRLAARLDAERYRAVMRAFAAGAEEVVASLRGRSEKFVGDAVMAVFGVPKVNED
                     DALRAVRAAMWIRGRAERLSQDLGLEEPLRLRIGLNSGPVAAGSGDGAQLLVSGAAVN
                     LAARLQQAAEPGEILAGDTTWRLTRDAVEYGDPRIVPARGFDEPVEAHPVRSLSTRSS
                     RRTIPFVGRSRERGLLLDTFERVRETSRPHLVTILGEPGIGKSRLAEEVLASVPEDVS
                     VLRGSAGASGDETLGPIAQMLRDELDLAGVASADDVRERLEEMVAGCCDAGEVERTTA
                     RLGLALGLGEEDRGGHPYRVGEVRSGMEGLVIGLGRRGPVALVFEDLHEAHQELLELI
                     EALVRGVRGSPLLALSLARDELLEKRSAWGAGIPDALILRLEPLPDEEAATLARAAGD
                     ALEEPTRERVVRLSGGNPYFIVETTGMLLERPPADGPARWVIPPTVQAAAASRIDHLD
                     EPARRLLRTASVFPGGRFQVGHLAAITAPDPGVLESLEEAELLVPDGRPGRWRFRHEM
                     LRHVAYESLAKRERVRLHLAVADHLEAQDVHLPQAIAHHVELAARASIDLGGTDPGLV
                     DRAVEALAAAGDATRRRIESRAAADLYRRGLDLAGPESGWGVREARMLAGLGEARYWL
                     GEFEQARAALVRALELGGEDPATQAAAGRFLADVLLNVDRDVDRATVMFETALSAARS
                     LDDPWTISRTLLMAGWAPYWRNDFEGARRSFSEALEVARKAGDAWAEARALVSLASVV
                     SSGGNQREVLELAEEALAIGRELDEPFTVAVAQQSVGNALRRMLRLDEALPHLRAAVA
                     GFRELDARWELASTLGDLAYVHRLAGRPEEADGPLREALALCLDLGERALTTWTAAEL
                     AFLLLGQGRVEEARELLEGDVPLEDQEPALGVSARMAKALLARMEGDEEAAREHASAA
                     VAMERERGLENQIATAVYWVGSTFGPDLVGGEDAVRRAEETLRQAGWLHAIREAELAR
                     QPKKTSATS"
     gene            3193..4227
                     /locus_tag="VF058_09165"
     CDS             3193..4227
                     /locus_tag="VF058_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEX6208509.1"
                     /translation="MAGIRVDLFSDTVTLPTEGMRKAIAEAEVGDEQKFEDPTVNLLQ
                     DRVADLLGAEAGLYMPSGTMCNEAAFAVHCRPGDEIIADRNAHPVIAEGGGPAVISGA
                     MVQGIEGERGVFTAQQVREAIRPPLRLSPRSRVMSVENTSNYGGGRCWPIERIREVTA
                     VAEEHGLAAHMDGARLLNAVVATGTAAAAYAEPFDTAWIDFSKGLGAPVGAVLVGSRD
                     LIEEAWRWKQRLGGAMRQAGIVAAACVYALDHHVDRLADDHENARALAEGLADLPGVG
                     LDPETVETNIVVFELEGMPAERFAGRLLDEHGVRVSTLGPSIVRAVTHLDVDREGVEA
                     ALAGARAILR"
     gene            complement(4220..4906)
                     /locus_tag="VF058_09170"
     CDS             complement(4220..4906)
                     /locus_tag="VF058_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208510.1"
                     /translation="MTEHTLVLEDRTTCPSCAAVNPSDSPFCWQCYAPFARTVPGAPA
                     WAPPDAAGETSEAVPSAGTPADPRRRWLGFALILALVAGGFLAWRALTGGPFPGAFRG
                     YERLETAEATRFEEGVSDLGEALGIEMHGAQYGHGSELAVLAIVAEEGASPQELGSRF
                     LEGPAPRPRDLLEVQRDGARFLCFPADAEVPGAGCTWIDRDSVGVVYGRFVNVEQQLE
                     LSAELRAAVI"
     gene            complement(4950..5789)
                     /locus_tag="VF058_09175"
     CDS             complement(4950..5789)
                     /locus_tag="VF058_09175"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX6208511.1"
                     /translation="MSRPLIGAHVPARGRPARAVDEAEARGAEAFQIFVSNPRAWALP
                     RISRGAAQEFRERREAAGSPPAFAHASYLVNIASPNPQFWRRSVELAMGEARVAAALA
                     LDGLVVHAGAGGPGERAAAVARAGAAATAIAEMGAPVLLELTAGGSGTVASTLTEAVD
                     LLEAAGGDPRLGIVVDTCHLFAAGTPLDDPRQARVALDPLDAPPLSGRLRLVHANDAR
                     DPRGSRRDRHEHVGEGHIGEESFRTILTHEAVRRVPVIIETPGKVEEDRRNIALLRRL
                     AGD"
     gene            complement(5786..>7682)
                     /gene="pknB"
                     /locus_tag="VF058_09180"
     CDS             complement(5786..>7682)
                     /gene="pknB"
                     /locus_tag="VF058_09180"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEX6208512.1"
                     /translation="VEPSLVVGHDPLQCGDEAAPPEMIETTVDGRYQILARVAAGGMG
                     EVFRAHDPVLNREVALKMLHPTLAGDTDFIDRFRREARSAAKLSHPNIVAVHDWGQAG
                     ETYFMVMEYIRGPNLRTVLADGVPLQPAQAAEVLSQVLAALGHAHEHGIVHRDVKPEN
                     ILITKQGVVKVADFGLARALAESRVTHAPGTVTGTVQYLAPEQIQGEPADPRTDLYAA
                     GVVLYELLVGRVPFTGETSVAIAYRHLQDPMPVPSSANPMVPTSLDEVVAHATEKDRE
                     RRVESAERMRKELANSTAELPPAPPLRALAEAAPSVDEVPPDRARTVTIPEAMPPRKR
                     RRRRLATFARVIALLAVLAAAGWATWTYLVPHRTDAPRLQGLTIEEAQGRAEEAQLAL
                     VVGGREYSSDVEAGRILSQEPGAGEVVERGTEIRVVLSRGPELVAIPDVTDGTLQEAR
                     RALRRAGLTWDVRHVYHPQAPEGTVVDQNPLAEREIEVGTQVIVTVSRGPRPISVPGV
                     EGRPASEAEAELEAAGLRVATTQEFSDRVPRGNVIRQSPEPEAQVLPGTTVELVVSRG
                     PREFPMPRVIGKDRDEAITELQRLGLEVETTRVPGTTGTRVVGQSPRAGTTVPAGSRV
                     TIYIGGG"
BASE COUNT          991 a         2870 c         2842 g          979 t
ORIGIN      
        1 aggtcgtccc gccggagctc tacgaggtgg ccgaggtctt cttcggctga cgcgccagct
       61 cggcctcccg gatcgcgtgc agccaccccg cctgccggag ggtctcctcc gcgcgccgga
      121 cggcgtcctc gcctccgacc aggtcggggc cgaaggtgct tccgacccag tagacggcgg
      181 tggcgatctg gttctcgagc ccgcgctctc gctccatggc caccgcggcg gaggcgtgct
      241 cccgggcggc ctcctcatcc ccctccatcc tggccagcag ggccttcgcc atccgcgcgc
      301 tgaccccgag cgccggctcc tggtcctcaa ggggcacgtc gccctccagc agctcccgcg
      361 cctcctcgac ccggccctgt cccaggagga ggaacgccag ctcggcggcg gtccacgtgg
      421 tcagcgcccg ctccccaagg tcgaggcaga gggcgagcgc ctcgcgcagg gggccgtcgg
      481 cctcctcggg cctccccgcg agtcggtgga cgtacgccag gtcgcccagg gtgctggcca
      541 gctcccatcg ggcgtcgagc tcccggaacc ccgccaccgc cgcccgcagg tgggggaggg
      601 cctcgtccag gcgcagcatc cgccgcaggg cgttccccac gctctgctgg gccacggcca
      661 cggtgaacgg ctcgtcgagc tcccgcccga tcgccagggc ctcctccgcg agctcgagca
      721 cctcccgctg attgccaccc gacgagacga ccgaggccag cgagacgagc gcccgggcct
      781 cggcccaggc gtccccggcc ttccgagcga cctccagggc ctcggagaac gagcgccgcg
      841 cgccctcgaa gtcgttccgc cagtacggcg cccacccggc catcagcagc gtgcgtgaga
      901 tcgtccaggg gtcgtccagc gagcgggcgg cggagagcgc cgtttcgaac atgaccgtcg
      961 cccggtcgac gtcccggtcc acgttcagca ggacgtcggc caggaagcgc ccggccgcgg
     1021 cctgcgtcgc cgggtcctcc ccgccgagct cgagggcgcg cacgagcgcc gcccgagcct
     1081 gctcgaactc gcccagccag tagcgggcct cgcccagccc ggccagcatc ctggcttccc
     1141 ggacgcccca cccggactcg ggccccgcca ggtccaggcc ccttcggtag aggtcggccg
     1201 cggcccgtga ctcgatccgg cgcctcgtcg cgtcgcccgc cgcggcgagg gcctccaccg
     1261 cccggtccac gagccccggg tcggtcccac cgagatcgat cgaggcccgg gccgccagct
     1321 cgacgtggtg ggcgatcgcc tgcggaaggt gcacgtcctg cgcctcgagg tggtcggcga
     1381 cggcgaggtg cagccggacg cgctcgcgct tggcgaggct ctcgtacgcg acgtgacgga
     1441 gcatctcgtg gcggaagcgc cacctccccg gtcgaccgtc ggggacgagg agctcggcct
     1501 cctccagcga ctccaggacg ccggggtcgg gagcggtgat cgccgccagg tgcccgacct
     1561 ggaacctccc gcccgggaaa accgacgccg tccgcaggag gcgccgggcg ggctcgtcca
     1621 gatgatcgat ccgggaggcc gcggcggcct ggaccgtggg cgggatcacc caccgggccg
     1681 gcccgtccgc gggcggtcgc tccaggagca tgccggtcgt ctccacgatg aagtaggggt
     1741 tgccccccga gaggcggacc acccgttccc gcgtcggctc ctcgagggcg tcgccggcgg
     1801 cgcgcgcgag cgtggcggcc tcctcgtcgg ggagcggctc caggcggagg atgagcgcgt
     1861 cggggatccc cgcgccccag gcggaccgct tctccaggag ctcgtcccgg gccagggaga
     1921 gggccagcag gggcgagccc cgtaccccgc gcacgagggc ctcgatcagc tcgaggagct
     1981 cttggtgggc ctcgtgcagg tcctcgaaga cgagcgcgac ggggccccgt cgcccgagcc
     2041 cgatgaccaa cccctccatc ccgctgcgga cctcgcccac gcggtaggga tggccgcccc
     2101 gatcctcctc gccgagcccg agggcgagcc ccagcctggc ggtcgtccgc tcgacctcgc
     2161 cggcgtcgca gcacccggcc accatctcct cgaggcgctc ccggacgtcg tcggccgagg
     2221 cgacgccggc gaggtcgagc tcgtcccgca gcatctgcgc gatcggcccc agggtctcgt
     2281 cgccagaggc cccggcgctg ccccgcagga cgctcacgtc ctcgggcacg ctggccagga
     2341 cctcctcggc caggcggctc ttgccgatcc cgggctcgcc caggatcgtc acgaggtggg
     2401 gtcgggacgt ctctcgcacc cgctcgaagg tgtccaggag caaacctcgc tcgcgggatc
     2461 gccccacgaa ggggatcgtc cgccgggaag acctcgtgga gagcgaccgg acggggtggg
     2521 cctcgacggg ctcgtcgaag ccccgggcgg gcacgatccg ggggtcgccg tactcgaccg
     2581 cgtcgcgggt caggcgccac gtcgtgtcgc cggccaggat ctcaccgggc tcggcggcct
     2641 gctggaggcg ggcggccagg ttcacggcgg cccccgagac gaggagctga gctccgtcgc
     2701 cgctgccggc cgccacgggc ccggagttca ggccgatccg gaggcggagc ggctcctcga
     2761 gcccgaggtc ctgcgagagc cgctcggccc ttccccggat ccacatcgcg gcgcggacgg
     2821 cccggagggc gtcgtcctcg ttcaccttgg gaaccccgaa cacggccatc acggcgtcgc
     2881 ccacgaactt ctccgagcgt ccgcggagcg aggcgacgac ctcctcggcc cccgcggcga
     2941 aggcccgcat cacggcccgg taccgctcgg cgtcgaggcg ggccgcgaga cgggtcgagc
     3001 cgatcacgtc ggcgaacagg acggtgacga gcttccgctc ctcgccctcc gccgcggcga
     3061 cggcggcgcc gcacgagggg cagaacctcg cgtcggcggg gaggagctcg ccgcagctgc
     3121 ggcaggcctg ttggctcatc ggaggccaag catagcgatg ggagcggcga gcggcgtgta
     3181 tcctcggcgg tcgtggccgg gatccgcgtg gaccttttca gcgacaccgt gaccctcccc
     3241 accgagggga tgcggaaggc gatcgccgag gccgaggtgg gggacgagca gaagttcgag
     3301 gaccccaccg tcaacctcct gcaggatcgg gtggccgacc tgctgggggc ggaggccggt
     3361 ctctacatgc cctcggggac gatgtgcaac gaggcggcgt tcgccgtcca ctgccggccc
     3421 ggagacgaga tcatcgccga ccgcaacgcc catcccgtca tcgcggaggg gggcgggccc
     3481 gcggtgatct cgggggcgat ggtgcagggg atcgaagggg agcggggcgt gttcaccgcc
     3541 cagcaggtcc gggaggcgat ccgtccgccc ctccggctct cgcctcgcag tcgggtgatg
     3601 tccgtcgaga acacctcgaa ctacggggga ggccgctgct ggccgatcga gcggatccgg
     3661 gaggtcacgg ccgtggcgga ggagcacggg ctcgccgcac acatggacgg tgcgaggctg
     3721 ctcaacgcgg tcgtggcgac cggcacggcg gcggcggcgt acgcggagcc cttcgacacc
     3781 gcctggatcg acttcagcaa agggctgggc gccccggtcg gcgccgtgct cgtcggctcg
     3841 cgcgacctca tcgaggaggc ctggcggtgg aagcagcggc tgggcggtgc gatgcggcag
     3901 gctgggatcg tggccgcggc ctgcgtctac gcgctggacc accacgtcga tcgcctggcc
     3961 gacgaccacg agaacgcgag ggccctggcc gagggcttgg cggacctccc cggcgtgggg
     4021 ctggacccgg agaccgtgga gacgaacatc gtcgtgttcg agctcgaggg aatgcccgcc
     4081 gagcggttcg ccggccgcct gctggacgag cacggggtcc gggtcagcac cctgggtccg
     4141 tcgatcgtcc gggcggtgac gcatctggac gtggatcggg agggcgtgga ggcggcgttg
     4201 gcaggggcgc gagcgatcct cagatgacgg cggcgcgcag ctccgccgag agctcgagct
     4261 gctgctccac gttcacgaag cgtccgtaga cgaccccgac cgagtcccgg tcgatccagg
     4321 tgcagcccgc gccaggcacc tccgcgtccg cggggaagca gaggaacctc gcgccgtcac
     4381 gctgcacctc gagcaggtcg cgcgggcgtg gggccggccc ctcgaggaac cgcgacccca
     4441 gctcctgagg cgaggcgccc tcctcggcca cgatggccag caccgcgagc tcggatccat
     4501 gtccgtactg tgccccgtgc atctcgatcc ccaaggcctc gccgagatcg ctcacgccct
     4561 cctcgaagcg ggtggcctcc gccgtctcga ggcgctcgta cccgcggaag gcccccggga
     4621 aggggcctcc ggtcagggcg cgccaggcga ggaaaccccc ggcgaccagc gcgaggatga
     4681 gggcgaagcc gagccacctg cgccgggggt cggcgggtgt cccggcggag gggacggcct
     4741 cagaggtctc gccggcggcg tcgggcgggg cccatgcggg cgccccaggg acggtccggg
     4801 cgaacggcgc gtaacactgc caacagaacg ggctgtcgga ggggttgacg gccgcgcagc
     4861 tgggacaggt cgtccgatcc tcgaggacga gcgtgtgctc ggtcatacgg cgcgtagtat
     4921 cggcatcccc tcggcgccct tgagctcggt cagtcgcccg cgagccggcg caggagggcg
     4981 atgttgcgac ggtcctcctc caccttcccc ggggtctcga tgatcacggg gacgcgccgg
     5041 acggcctcgt gggtcaggat cgtccggaag ctctcctcgc cgatgtggcc ctccccgacg
     5101 tgctcgtggc ggtcgcggcg agagccacgg ggatcccggg cgtcgttggc atggaccagg
     5161 cggagccggc ccgagagcgg cggcgcgtcg agagggtcaa gggcgacgcg cgcctgccgg
     5221 ggatcgtcga gcggggtccc ggcggcgaag agatggcacg tgtcgaccac gatcccgagg
     5281 cgcgggtccc cgccggccgc ctcgaggagg tccaccgcct ccgtgagtgt ggaggccacc
     5341 gtgcccgagc cgccggcagt cagctcgagc agcacggggg cgcccatctc ggcgatcgcg
     5401 gtggcggcgg cgccggcccg ggccacggcc gcggcccgct cgcccgggcc ccccgcgccc
     5461 gcgtgcacga cgaggccgtc cagggccagc gccgccgcca ccctcgcctc gcccatcgcc
     5521 agctccaccg accgtcgcca gaactggggg ttgggggagg cgatgttcac gaggtaggag
     5581 gcgtgggcga acgcgggcgg gctcccggcg gcctcccgcc gctcgcggaa ctcctgggcc
     5641 gcgccccgcg agatgcgggg cagcgcccac gccctcgggt tcgacacgaa gatctggaag
     5701 gcctcggcgc cccgcgcctc cgcctcgtcc acggcccggg cgggacgccc acgggccggg
     5761 acgtgggccc cgatgagggg ccggctcatc ccccgccgat gtagatcgtg acccggctcc
     5821 cggccgggac cgtcgtcccg gcccgtgggc tctgcccgac cacgcgggtc ccggtggtcc
     5881 cgggaacgcg ggtggtctcg acctccaggc ccaggcgctg gagctcggtg atcgcctcgt
     5941 ccctgtcctt cccgatcact cgcggcatcg ggaactcccg gggaccccgc gagaccacga
     6001 gctccaccgt cgtcccgggg aggacctgcg cctccggctc gggggactgc cggatgacgt
     6061 tcccccgggg cacgcggtcg gagaactcct gcgtcgtggc cacacgcaga ccggcggcct
     6121 ccagctccgc ctcggcctcg gacgcgggcc gcccctcgac gcccgggacg ctgatgggac
     6181 gcggcccccg gctgaccgtg acgatgacct gcgtccccac ctcgatctcc cgctccgcga
     6241 gcgggttctg atcgaccaca gtgccctcgg gggcctgcgg gtggtagacg tggcgcacgt
     6301 cccaggtcag cccggctcgg cggagcgccc gccgcgcctc ctggagggtc ccgtccgtga
     6361 cgtccgggat cgccaccagc tcggggccgc gggagagcac cacgcggatc tcggtgccgc
     6421 gctccacgac ctcgccggcc cccggctcct gggacaggat gcggcccgct tcgacgtcgg
     6481 aggagtactc acggcccccg accacgagtg ccagctgcgc ctcctccgcc cgaccctgcg
     6541 cctcctcgat cgtcagcccc tggaggcggg gcgcgtcggt acggtggggg accaggtagg
     6601 tccacgtcgc ccatcccgcg gcagccagca cggccagcag cgcgatcacg cgggcgaacg
     6661 tggcgaggcg ccggcggcgg cgcttcctgg gcggcatggc ctcggggatc gtcacggtcc
     6721 gggcccggtc gggcggcacc tcgtccaccg agggcgcggc ttcggccagc gcccgaagcg
     6781 ggggcgcggg aggcagctcg gcggtggagt tcgcgagctc cttccgcatc cgctcggcgc
     6841 tctccactcg gcgctcccgg tccttctcgg tggcgtgcgc cacgacctcg tccagggagg
     6901 tcgggaccat cgggttggcg ctcgagggga ccggcatcgg gtcctgcagg tgccggtagg
     6961 cgatcgccac cgaggtctcg ccggtgaacg ggacccggcc gaccagcagc tcgtagagga
     7021 cgaccccggc cgcgtagagg tcggtgcgcg gatcggccgg ctccccctgg atctgctcgg
     7081 gcgccaggta ctgcaccgtg ccggtgacgg tccccggcgc gtgcgtcacc ctcgattcgg
     7141 ccagggcgcg ggccagcccg aagtcggcca ccttcaccac gccctgcttc gtgatgagga
     7201 tgttctcggg cttgacgtcc cgatggacga tgccgtgctc gtgcgcgtgc cccagcgcgg
     7261 cgagcacctg ggagagcacc tcggcggcct gcgccggctg gagcggcacc ccgtcggcca
     7321 ggaccgtccg gaggttgggg ccccggatgt actccatcac catgaagtaa gtctcgccgg
     7381 cctgccccca gtcgtggacg gcaacgatgt tcgggtggga gagcttcgcc gccgagcgcg
     7441 cctcccgccg gaaccgatcg atgaagtcgg tgtcccccgc gagggtgggg tgcagcatct
     7501 tcagcgcgac ctcgcggttg aggacgggat cgtgcgcccg gaagacctcg cccatgccgc
     7561 cggcggcgac cctggcgagg atctggtagc gtccgtctac ggtcgtctcg atcatctcgg
     7621 ggggagcggc ctcgtctccg cattgtaggg ggtcatgccc gactaccagg gaaggctcga
     7681 cc
//
LOCUS       DASUTW010000273         2901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1645197, whole genome shotgun sequence.
ACCESSION   DASUTW010000273 DASUTW010000000
VERSION     DASUTW010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645197"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(483..1091)
                     /locus_tag="VF058_09185"
     CDS             complement(483..1091)
                     /locus_tag="VF058_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208513.1"
                     /translation="MAELTRRRATSPWREGWGRTIGIGVVAGIVASAIMGVFAMIAAA
                     TYQDIGFFTPLHHIATPLVGDDAMMASMEAAARGELFELDVGPALVGLGVHMMVGAVW
                     GLLFFGLARAIGLRGAAVLVPAGIAFGLLVMLFMSWVTLPITAEVVGGGEAVEDIPSM
                     VGWGTFTVEHVLFGFVLGTWAAARRSAMARHAADREEPRLAA"
     gene            1206..1928
                     /locus_tag="VF058_09190"
     CDS             1206..1928
                     /locus_tag="VF058_09190"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX6208514.1"
                     /translation="MAALALGHEARVMVHEAAWVARCVGRGEWAPLSNGDLEDLAARL
                     STRIYEAGEQLFAQGQEPDGVYIVREGRVELVWRGSMRRRLVVQILHPGDVEGDICTI
                     LAIAPPYSARALDRVEALVISPADLEALLTRPQVSRRWLSSVAARLVHSQRRILQLQD
                     HEVRGKVASLLLDEERGGVVELPQESLAALLSVRRQAVNGALREFERAGLVRTSYRLV
                     RIEDRAGLESVAGRRASREHAA"
     gene            complement(2106..2492)
                     /locus_tag="VF058_09195"
     CDS             complement(2106..2492)
                     /locus_tag="VF058_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6208515.1"
                     /translation="MPSEGTDRPRAGDVAAREVPTCRPTERVSDVAARVASAGWDRCV
                     VINEERVVFGLLRSEELRAEDDATVEEVMRPGPSTFRPHVPIREMADFMAEHDLSSAP
                     ITTSDGRLVGLLTREDAERAAKETRK"
     gene            complement(2589..>2901)
                     /locus_tag="VF058_09200"
     CDS             complement(2589..>2901)
                     /locus_tag="VF058_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX6208516.1"
                     /translation="EARSVRKPTLLAIDSSTERPSLRVRICGQPLDAGGRMPRNVDRE
                     TVRRLVAEGAQLVDVLPASEYREDHLPGAINLPLERLDDEWKQVLDPGRPVVVYCWDS
                     A"
BASE COUNT          443 a         1042 c         1005 g          411 t
ORIGIN      
        1 gatgaacaac aagccgaaca ggatccgtcc gatcacgaac agggcgtcca tgcacgtcac
       61 ctcccgacac ggagaatccc tttccagccg catgaacgcc catcgcgccc gatctcttcc
      121 cgcgggggcg ctcccggaat cgtggctcgg cgggcccccc gccacgggaa ccaaccacgg
      181 accttgtccg ggcccggaca gttggggcgg gcatcgaccg gggtacggga acgacctgag
      241 gcgggagacg ccgacgagag gaggcgaaca tggcgatggc atgcggcaag tgcggggcgc
      301 aattcgatac tcaggaagag ctggacaaac acgtccggga gcaacacccg gaggaatcga
      361 aggaggatga acccgaaggc gggtgagcct cgccggggga gtccttcagc gaaacggccc
      421 ctccgccgtc cgatccccgg cggcggaggg gcgtcctctt gtccggccac gttggacgct
      481 gctcaggccg cgagccgggg ttcctcgcgg tccgccgcgt gccgggccat cgcgctccga
      541 cgagccgcgg cccaggtccc gaggacgaag ccgaacaaca cgtgctcgac ggtgaaggtg
      601 ccccagccga ccatgctcgg tatgtcctcg acggcctcgc cgccgcccac gacctcggcc
      661 gtgatcggca gggtgaccca gctcatgaag agcatcacca ggaggccgaa ggcgatgccc
      721 gccgggacca acacggcggc gccgcgaagg ccgatcgccc gcgcgaggcc gaagaacagg
      781 aggccccaaa cggcgcccac catcatgtga acccccaggc cgacgagcgc cgggcccacg
      841 tcgagctcga acagctcgcc tcgtgcggcc gcctccatcg aggccatcat ggcgtcgtcc
      901 ccgaccaggg gggtggcgat gtggtgcagg ggcgtgaaga acccgatgtc ctggtacgtc
      961 gcggccgcga tcatggcgaa cacccccatg atcgccgagg ccacgatccc cgcgaccaca
     1021 ccgatgccga tcgttcgtcc ccatccttct cgccacgggc tcgtggcccg tcgccgggtg
     1081 agctcggcca tcacacgtca cctccttgac ctttcgctac ccaagtccgg ccgggggtcg
     1141 tttgggattg tccgggaacg gacagatagg cacgaaggac cgcgtcggca gaagggaggc
     1201 acccgatggc tgctctcgcg ctggggcatg aggcccgggt catggtccac gaggcggcgt
     1261 gggtcgcccg gtgtgtgggc cgtggggaat gggctccgct ctcgaacggc gacctcgagg
     1321 atctcgccgc ccggctttcc acccgcatct acgaggccgg cgagcagctc ttcgcccagg
     1381 ggcaggagcc ggacggcgtg tacatcgtgc gcgagggccg cgtcgagctc gtgtggcgcg
     1441 gctcgatgcg ccggcggctg gtcgtccaga tcctgcaccc gggcgacgtc gagggcgaca
     1501 tctgcacgat cctggcgatc gcgccgccgt attcggcccg ggcgctcgac cgtgtcgagg
     1561 ccctcgtgat ctcccccgcg gacctcgagg ccctcttgac gcgccctcag gtgtcgcgcc
     1621 ggtggttgtc cagcgtcgcc gctcgcctgg tgcattcgca gcgacggatc ctccagctcc
     1681 aggaccacga ggtccggggg aaggtggcct cgctcctgct ggacgaggaa cgcggcggag
     1741 tggtggagct cccgcaggag tcgctggccg cgttgctctc ggtccggcga caggcggtca
     1801 acggcgccct tcgggagttc gagcgagcgg ggctcgtccg aacgtcctac cggctcgtcc
     1861 gcatcgagga ccgagccggc ctggagtcgg tggccgggcg acgggcgtcc cgagagcacg
     1921 ccgcctgacg tcaccctccg tgggcgtgcg gctcggggac ggcctgccgg agagccaggt
     1981 tcacgcagcg gtagcacaag gccgttccgc attcggctcg ttcgcagagc tcacagcggt
     2041 cgatgcccgc ccgcagcccg tgcagacgag gacggttgga tccggcatcc cgccgtcggc
     2101 cgagctcatt tccgagtctc cttcgccgcc cgctctgcat cctccctcgt gagcaggccc
     2161 accagcctcc cgtccgacgt cgtgatcggg gcgctcgaca ggtcgtgttc cgccatgaag
     2221 tcggccatct cccggatggg gacatggggg cggaacgtgc tcggacccgg cctcatgacc
     2281 tcttcgaccg tcgcgtcgtc ctcggcccgc aactcctcgg agcgcagcag gccgaacacc
     2341 acccgctcct cgttgatcac cacgcaccgg tcccaaccgg cggaggcgac gcgagccgcg
     2401 acgtccgaga cccgttcggt cgggcggcag gtcgggacct cccgggcggc gacgtcgccg
     2461 gcgcgcggcc ggtccgtgcc ttcgctgggc aagccggccg ccagccagtc gagcttgccc
     2521 tcgacgtagt cgtagacctc ggcgacaccg agcgcttcga gccgccacgc ggctcgtggg
     2581 ctcaggtctc aggccgagtc ccagcagtag acgaccaccg ggcgccccgg gtcgagcacc
     2641 tgcttccact cgtcgtcgag gcgctccagc ggcaggttga tcgccccggg caggtgatcc
     2701 tcccggtatt cggaggccgg gagcacatcg acgagctgcg ccccctccgc gaccaggcgg
     2761 cggacggtct cgcggtcgac gttcctcggc atgcggcctc ctgcgtccag cggctgtccg
     2821 cagatgcgta cccggagcga aggccgctca gtcgagctgt cgatggccaa gagtgtcggc
     2881 ttgcggacac tccgcgcctc g
//
LOCUS       DASUTW010000274         8269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_738449, whole genome shotgun sequence.
ACCESSION   DASUTW010000274 DASUTW010000000
VERSION     DASUTW010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8269
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738449"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VF058_09205"
     CDS             complement(<1..488)
                     /locus_tag="VF058_09205"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEX6208517.1"
                     /translation="MRASDILEAIGNTPLVGIQRLSPKPEVRLWAKLEGQNPTGSLKD
                     RIAKAMIEAAEASGELTPDRVILEPTSGNTGIALAMVARRKGYRMMVVMPENASPERV
                     QLLELYGAEIVFSPADAGTNGSIEVARRMAQDPRYFMPFQYGNPANPRAHHEGTGAEI
                     VR"
     gene            974..1156
                     /locus_tag="VF058_09210"
                     /pseudo
     CDS             974..1156
                     /locus_tag="VF058_09210"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            1140..1847
                     /locus_tag="VF058_09215"
     CDS             1140..1847
                     /locus_tag="VF058_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208518.1"
                     /translation="MNPYGELLERAGRRFPPPDAGLDDLHDRLRRRRRGRRIVAGAVS
                     LALFVGTAAALGSRIPFVGSERSGAPADVDVVVSRILPDRVPPLALKYESHRGTEAMT
                     GLVVGDTLVRLASLPATEGATRVFQRNDPLLIVAVLALRFEDARSAQIAYALLEGDVR
                     RGDPDAPTVTEPDPRLGVESVALVSASNRRYRGARTTALLWQRGDLVMGALTIGRAPR
                     ERLERIARDMDDRAQTG"
     gene            complement(1857..2126)
                     /locus_tag="VF058_09220"
     CDS             complement(1857..2126)
                     /locus_tag="VF058_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD family protein"
                     /protein_id="HEX6208519.1"
                     /translation="MISVKLPTLLRGHAGGRPEVQSEGGSLGELLENLERRYPGLTDG
                     LRDGRGLRRHVNVYVNGEDVRYTGGLDTSLGEGDTVMILPAVAGG"
     gene            complement(2150..2983)
                     /gene="moeB"
                     /locus_tag="VF058_09225"
                     /pseudo
     CDS             complement(2150..2983)
                     /gene="moeB"
                     /locus_tag="VF058_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897946.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
     gene            complement(2980..3411)
                     /locus_tag="VF058_09230"
     CDS             complement(2980..3411)
                     /locus_tag="VF058_09230"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEX6208520.1"
                     /translation="MTIEIPRDRYEEVLAHLRAGRPNEACGVLAGVDGKVAAVYPMRN
                     AEESPVAYRFDEREQIEVFREVEERGWELLAFFHSHTHTEAYPSPTDVRHAHWEGSDG
                     EPLPAYPGTRYVIVSLMGEEPDVRAFAFEGGAPVEEEVRVV"
     gene            complement(3440..4489)
                     /gene="selD"
                     /locus_tag="VF058_09235"
     CDS             complement(3440..4489)
                     /gene="selD"
                     /locus_tag="VF058_09235"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4442..4444),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX6208521.1"
                     /translation="MRDEVRLTRYSHGAGUACKLGPSDLSQVLGQLGPTEYPAEVVVS
                     QETRDDAAVYRLPDGTGLVQSVDFFTPIVDGARDWGRIAAANALSDLYAMGARPTLAL
                     NLVGWPREELPLDLLAEVLRGGAEIVARAGAVVVGGHSIDDPEPKYGMAVTGFVELDR
                     LVRNSTAPPGATLVLTKPLGLGILTTGIKRERATEEQAAAAVETMTTLNDRAAEAMVE
                     TGASAATDVTGFGLLGHLHEMLAASSVAAEVDAASVPFLAGALELAEEGVVPGGTRRN
                     AAFLDPLVDRDLDEHEWLALVDAQTSGGLLIATRSPDALTAALEARGVAGASIGRVVE
                     GEPGRIGVLGRPTGR"
     gene            4549..4944
                     /locus_tag="VF058_09240"
     CDS             4549..4944
                     /locus_tag="VF058_09240"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX6208522.1"
                     /translation="MARPARQVALILARDLASQLSTPMFIVDTEGTVIYFNEPAEAIL
                     GKTWAETGELSAEEWGTIFRQRTLEGEPLPREQIPLSIALVEKRPAHKSLRITGMDGV
                     EHTISSTAIPLMAHPDEIVGAVAIFWVTD"
     gene            4944..5804
                     /locus_tag="VF058_09245"
     CDS             4944..5804
                     /locus_tag="VF058_09245"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6208523.1"
                     /translation="MRATIRGCRGSLAAPGPGTVRYGGNTSCVEVQTAGDALIVLDAG
                     TGIRLLGLELEESCPPVIHLMLTHLHLDHLEGLGFFAPLYIPEIEIHLWGPPSPVQSL
                     RARVERYLSPPLFPVELSDIPSRVMFHDVPEEPWEIGGATISAAPLKHPGPTVGYRIE
                     EEGRSIVYMPDHEPALGSDLRRVPPSWISGYDLAEGADVLMHDAQYTAEEYTERLGWG
                     HSALEHVVDFALITKVERLVMTHHDPNHDDDELERMLAEARGMWGEGPGDLVLAREGM
                     TIVPGEDVSP"
     gene            5865..6425
                     /locus_tag="VF058_09250"
     CDS             5865..6425
                     /locus_tag="VF058_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208524.1"
                     /translation="MANVFDRAKEAVIGVVKGAGDIVNALRSTVTDVVVQTLKEAGEI
                     TGTALGVVTDAVRGAIRGTKEAGAEATESARAIVSGAIEAVAQVGGDVVTGARNAVRG
                     AIQGAVEVGGDVATAAVGAVEGAVETAGKVGQDVMIVGKAAIETAVETAGTVASGSVD
                     AVKQGLRSSAAVPREVVESALGKDES"
     gene            6464..7591
                     /locus_tag="VF058_09255"
     CDS             6464..7591
                     /locus_tag="VF058_09255"
                     /inference="COORDINATES: protein motif:HMM:NF019522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEX6208525.1"
                     /translation="MTLGTVAAAIAAATVLGIIALRPRGEPDANLRELGIASEVYGAA
                     ITSVEVGPCPGAEEAGGVRCRLVGARLLQGPDEGRTVTISLSDTPSTPAFEVGEEIVL
                     AHTPEAQEGFRYQFADRQRRPVLWILFGLFVLAVTVLGGLRGIAALAGLGASVVLLIL
                     FVLPSILDGRSPLAVAVVGASAIAFLALYLAHGFTLMTTVALLGTVASLALTTVLAEL
                     FVGVARISGLATEEATIVQVGAAEIDLAGLILGGVVIGALGAIDDMTVTQASTVWELH
                     GANPRLGKRGLFRSGMRVGRDHVASTVNTLLLAYAGASMPLLLLFVLSEQSLGTVANS
                     EVVATEIVRTLVGSIGLVASVPITTWLASWVVGASPAPEAA"
     gene            7588..>8269
                     /locus_tag="VF058_09260"
     CDS             7588..>8269
                     /locus_tag="VF058_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX6208526.1"
                     /translation="MSELRVFDLWGVPFDGGATLGWPGARNGPDRVRRALSWMAMREE
                     EGLVRAVDPGEDLPVPEVADRGDVDVVPHDLMATIDAVSDAVTGSVTASRVPIVVGGE
                     DSLFFPSVRGLHDAVEGSVGVIHFDAHFDLLDENRRQGRFSHSSGMRRSLELERVAAG
                     HCVQVGTRTFNFPSSAAFVEKIGLLQIPAVEVHEQGVTGTVERVLERVGGVDHVFWSL
                     DIDVIDPAH"
BASE COUNT         1142 a         2937 c         2993 g         1197 t
ORIGIN      
        1 tcccggacga tctcggcccc ggtcccctcg tggtgggccc gagggttcgc ggggttgccg
       61 tactggaacg gcatgaagta gcgggggtcc tgggccatgc ggcgggccac ctcgatcgag
      121 ccgttcgtcc cggcgtcggc cggggagaag acgatctccg ccccgtacag ctccaggagc
      181 tggacccgct cggggctggc gttctcgggc atcaccacca tcatccggta ccccttccgg
      241 cgggccacca tcgccagcgc gatccccgtg ttcccggagg tgggctcgag gatcacgcga
      301 tcgggggtga gctcgccgct ggcctcggcc gcctcgatca tggccttggc gatgcggtcc
      361 ttcagggagc ccgtggggtt ctggccctcg agcttggccc agagccggac ctccggcttg
      421 ggagagaggc gctggatccc gaccagcggg gtgttcccga tcgcctcgag gatgtcgctc
      481 gctcgcatcc gccgattatc ccagccgggg ggcgcgggat ggccgccgcc tcgatccggt
      541 gcgacgatgt aagcggatcg aggtggtcgt gcgtccatgc gcgggatggg tgaacggatc
      601 acgaccgtga gcgcggccgc gccggccgtg atggaggttg tccccgtgga cggacccggc
      661 acgcagccgg ccatctcctt cgaggatctc taccgggagg agcatgagcg gctgtaccgg
      721 gccctctgcc tggtgacgag gaaccgccac gaggcggagg acctcgtcca ggaggcgttc
      781 ctccgcgtgc tggagcgatg ggagcggccg ggagcgatcg gcgatcccgt cgggtacctc
      841 taccggacgg cgatgaacgc cttccgatcc gggtaccgac gggctgcgct ggccctgaag
      901 cgaggcatcg gggtcgtccc ccccccccga cgacgagctc gagcggctcg agggcaccga
      961 ctgggtcctc gcgacgctgg ccccgctcag cagacgccag cgggccgcgg tgatcctcgt
     1021 ggacctcctc ggatgctcgt cggaggaggc cgggcggatg ctctcgctga gcgcctcgac
     1081 ggtccgcgtc cacgtggccc gggcccacgc cgcgctccgg cagcggatgg aggtggacga
     1141 tgaacccgta cggtgagctg ctggagcggg cgggacggcg gttcccgccc cccgacgcag
     1201 ggctggacga cctccacgac cgtctgcgcc gtaggcgccg gggccggcgc atcgtggcgg
     1261 gggcggtcag cctcgctctc ttcgtgggga ccgcggccgc gctcggctcg cggatcccct
     1321 tcgtcgggag cgagcggtcc ggagcgcccg ctgacgtgga cgtggtcgtc tcgcggatcc
     1381 tccccgaccg tgtcccgccg ctggcgctga agtacgagag ccaccggggg acggaggcca
     1441 tgacggggct cgtggtgggc gacacgctcg tgcggctcgc gagcctcccc gcgaccgagg
     1501 gggcaactcg ggtcttccag aggaacgacc ccctcctcat cgtggccgtc ttggccctcc
     1561 ggttcgagga cgcgcgctcc gcccagatcg cctacgccct gctcgagggc gacgtccgaa
     1621 gaggggaccc ggatgcgccg accgtgacgg agcccgaccc gcgcctcggg gtggagtccg
     1681 tcgccctcgt cagcgccagc aatcgccggt accgaggcgc ccgaacgacg gcgctcctgt
     1741 ggcagcgagg ggacctcgtc atgggggcac tgaccatcgg cagggctcct cgcgagcgcc
     1801 tcgagaggat cgcacgggac atggacgatc gggcgcagac gggctgacgg gtcggctcag
     1861 ccaccggcga cggcgggaag gatcatgacc gtgtcgccct cgcccagcga cgtgtcgagg
     1921 cctcccgtgt accggacgtc ctccccgttg acgtagacgt tgacgtgccg ccggagacct
     1981 cggccgtccc ggaggccgtc cgtcaggccc gggtaccgcc gctccaggtt ctccagcagc
     2041 tcgccgaggc tcccaccctc gctctgcacc tccggccgac cccccgcgtg gccgcggagg
     2101 agggtgggga gcttcacgct gatcacgagg ccacctcggg cgcccgctcg gcctcgggcg
     2161 gcgcctcacc cggcgtcagg ccgcagaacg cctcgtagtc gatcagctcg gtcaccgagg
     2221 gctcctcacc gcagaccggg caggcggggt cccaccgcag gttcatcgtt cggaactcca
     2281 tcgcgagcgc gtcgagcagc aggagccgtc ccgcgagcgg ctccccgatc cccgcgacga
     2341 gcttgatcgc ctccgtcgcc tggatcgacc caacgatccc cggcagcacg ccgagcacgc
     2401 cggcctcggc gcacgagggc acggtcccgg gcggcggggg ctccgggaag aggcatcggt
     2461 agcagggatg ctcccggccg gggaggaaca cgctggcctg cccctcgaac tggtagatcg
     2521 acccgtacac gagcggcttc cccaggatct gcgaggcgtc gttcgccagg taccgcaccg
     2581 ggaagttgtc ggtcccgtcg acgatcacgt cgtagggctc gaagatctgg aacgcgttct
     2641 gcgaggtgag cggcgtgtcg tgggccacga cctcgatcgt ggggttgaga tcgagcaggc
     2701 gctccctccc ggactcgacc ttggggcgcc ccacgtcgtc cgtgtcgtgg aggagctgcc
     2761 gctggaggtt cgtcacgtcg accttgtcga agtcgaggag gccgatccgc cctacccccg
     2821 ccgcggcgag gtacatggcc accggggacc ccaggccgcc ggccccgatc acgagcaccg
     2881 agccgtcggc gagcctcccc tgcccctcgg ccccgagctg gggcaggaag atgtgccgcg
     2941 cgtacctcag gacctgctcc tccgtgaacc ggggctcggt catacgaccc gaacctcctc
     3001 ctcgaccggg gctcccccct cgaaggcgaa cgcgcggacg tcgggctcct cccccatcag
     3061 cgagacgatg acgtagcggg ttcccggata cgccgggagc ggctcgccgt ccgagccctc
     3121 ccagtgcgcg tggcggacgt ccgtgggcga cgggtaggcc tcggtgtggg tgtgcgagtg
     3181 gaagaaggcc agcagctccc agccccgctc ctcgacctca cggaagacct cgatctgctc
     3241 ccgctcgtcg aaccggtagg cgaccgggct ttcctccgcg ttgcgcatcg ggtagacggc
     3301 ggccaccttg ccgtccacgc cggcaagcac gccgcacgcc tcgttgggcc tgccggcgcg
     3361 gaggtgggcc agcacctcct cgtaccggtc ccgcgggatc tcgatcgtca cgacgctaat
     3421 cctagcaaca gggtcggatt caccgtccgg tcgggcgacc caggacgccg atccggcccg
     3481 gctccccttc cacgaccctc ccgatcgagg cgcccgcgac gccacgcgcc tcgagagcgg
     3541 cggtgagggc atcggggctc ctcgtggcga tcagcaggcc gccggacgtc tgtgcgtcca
     3601 cgagggccag ccactcgtgc tcgtcgaggt cccggtccac gaggggatcg aggaaggccg
     3661 cgttgcgccg ggtgccgccc gggaccaccc cctcctccgc gagctcgagg gcgccggcca
     3721 ggaagggcac cgacgccgcg tcgacctcgg cggccaccga cgaggccgcg agcatctcgt
     3781 ggaggtgccc caggagaccg aacccggtca cgtcggtggc cgccgaggcc cccgtctcga
     3841 ccatcgcctc cgccgcgcgg tcgttcagcg tggtcatcgt ctcgacggcc gcggccgcct
     3901 gttcctccgt ggcccgctcg cgcttgatcc cggtcgttag gatccccagg cccagcggct
     3961 tcgtcaggac gagcgtggcg cccggcggcg ccgtgctgtt ccggacgagg cggtcgagct
     4021 ctacgaaccc cgtgacggcc atcccgtact tcggctccgg gtcgtcgatc gagtgcccgc
     4081 ccactacgac ggcgcccgcc cgggccacga tctcggcgcc cccccgcagg acctccgcca
     4141 gcaggtcgag cggcagctcc tcccgcggcc acccgacgag gttcagcgcc agggtgggac
     4201 gggcccccat cgcgtagagg tccgagaggg cgttggccgc ggcgatccgc ccccagtccc
     4261 gggccccgtc cacgatgggc gtgaagaagt ccaccgactg cacgagcccc gtgccgtcgg
     4321 gcaggcggta gacggccgca tcgtctctcg tctcctggga gacgacgacc tcagccgggt
     4381 attcagttgg gccgagctgc cccagcacct gggacaggtc cgagggaccg agcttgcacg
     4441 ctcacccggc gccgtgactg tatctcgtca ggcgcacctc gtcgcgcatg ggccgaggat
     4501 acccgcgggg ggttgagcgg gacccgatcc ctcgctaggc tccccgcaat ggcacgccca
     4561 gcacggcagg tcgctctgat cctggcccgg gatctcgcct cgcagctgtc cacccccatg
     4621 ttcatcgtgg ataccgaggg cacggtgatc tacttcaacg agcccgcaga ggcgatcctg
     4681 gggaagacct gggctgagac gggcgagctc tcggccgagg aatggggaac gatcttccgg
     4741 cagcggacgc tggagggcga gccgctcccc agggagcaga tccccctctc gatcgccctc
     4801 gtcgagaagc gcccggcgca caagtccttg cggatcacgg ggatggacgg ggtggagcat
     4861 acgatctcct ccacggcgat cccgctcatg gcccaccccg acgagatcgt tggcgcagtc
     4921 gcaatcttct gggtcaccga ctgatgcggg ccacgatccg agggtgccgt ggctccctgg
     4981 cggctccggg acccgggacc gtccgctacg ggggcaacac ctcatgcgtc gaggtccaga
     5041 ccgcaggcga cgcgctcatc gtgctcgacg cgggaacggg catccgcctc ctcggcctcg
     5101 agctcgagga gtcgtgtccg cccgtgatcc acctcatgct gacccacctg cacctcgacc
     5161 acctcgaggg gcttgggttc ttcgcccccc tgtacatccc cgagatcgag atccatctgt
     5221 ggggcccgcc ctcccccgtt cagagcctgc gcgcgcgcgt ggagcggtac ctgtccccgc
     5281 ccctcttccc cgtcgagctg agcgacatcc cctcccgcgt gatgttccac gacgtgcccg
     5341 aggagccctg ggagatcggc ggcgcgacga tctccgccgc ccccctgaag caccccgggc
     5401 ccacggtcgg gtaccggatc gaggaggagg ggcgctccat cgtgtacatg cccgaccacg
     5461 agcccgcgct cggctccgac ctcaggcggg tgccgccctc gtggatctcg ggctacgacc
     5521 tcgccgaggg cgccgacgtg ctgatgcacg acgcgcagta cacggccgag gagtacaccg
     5581 agcgccttgg gtgggggcac tccgctctgg agcacgtggt ggacttcgcc ctcatcacga
     5641 aggtggagcg cttggtcatg acgcaccacg atcccaacca tgacgacgac gagctcgagc
     5701 ggatgctcgc cgaggcccgc gggatgtggg gcgagggccc cggcgacctc gtcctcgcgc
     5761 gcgaggggat gacgatcgtc cccggcgagg acgtctcgcc ctgatcgcga gcggggaacg
     5821 cgccggacta tagtcagaca accgaatcga tcggaggtgc agtcgtggcc aacgtcttcg
     5881 accgcgcgaa ggaggccgtg atcggcgtcg tcaagggcgc gggcgacatc gtcaacgcct
     5941 tacgctccac cgtcaccgac gtcgtggtac agaccctgaa ggaagcgggc gagatcaccg
     6001 gcacggcgct gggcgtcgtg accgacgccg tccgcggcgc gatccggggc acgaaggagg
     6061 ccggcgccga ggccaccgag agcgcccggg cgatcgtatc cggtgcgatc gaggcggtgg
     6121 cccaggtcgg cggggacgtg gtcaccgggg ccaggaacgc cgtccggggc gcgatccagg
     6181 gagcagtgga ggtcggcggc gacgtggcca ccgcggcggt cggcgccgtg gagggcgccg
     6241 tcgagacggc cggcaaggtc ggccaggacg tcatgatcgt ggggaaagcc gcgatcgaga
     6301 cggcggtcga gaccgcaggg accgtcgcgt cggggtcggt ggacgcggtg aagcagggcc
     6361 tgcgatcgtc cgccgccgtt ccgcgcgagg tcgtggagtc ggccctgggg aaggacgagt
     6421 cgtagcgcac aggatcgagg ggcggatccc ccgaagggtc cgcgtgacct tggggacggt
     6481 ggccgcggcg atcgccgcgg ccaccgtcct cgggatcatc gccctccggc cccggggcga
     6541 gcccgacgcg aacctccggg agctggggat cgccagcgag gtctacgggg ccgcgatcac
     6601 aagcgtggag gtcggtccct gcccgggcgc cgaggaggcg ggcggggttc gctgccgcct
     6661 ggtcggcgcc cgcctcctgc aggggcccga cgagggccgg acggtgacca tcagcctctc
     6721 cgacaccccc tccacgcccg ccttcgaggt cggcgaggag atcgttctgg ctcacacgcc
     6781 cgaggcgcaa gagggctttc ggtaccagtt cgccgaccgc cagcgtcgcc ccgtgctctg
     6841 gatcctcttc gggctgttcg tgctcgccgt gacggtgctc ggaggactcc gcgggatcgc
     6901 ggcgctggca ggtctggggg ccagcgtcgt actgctgatc ctcttcgtgc tcccctcgat
     6961 cctggacggc cggagcccgc tggccgtggc ggtggtgggc gcctcggcca tcgcgttcct
     7021 ggccctgtac ctggcccacg gcttcacctt gatgaccacg gtggccctgc tggggacggt
     7081 ggcgagcttg gcgctgacca ccgtgctggc cgagctcttc gtgggggtcg ctcgcatcag
     7141 cggcctcgcc accgaggagg ccacgatcgt gcaggtgggc gcggccgaga tcgacctggc
     7201 gggactgatc ctcggcgggg tggtgatcgg cgcgctcgga gcgatcgacg acatgacggt
     7261 gacccaagca tcgacggtct gggagctcca cggcgcgaac cctcgcctcg ggaagcgagg
     7321 cctgttccga tccgggatgc gggtgggacg cgaccacgtc gcctccacgg tgaacacgct
     7381 gctgctcgcc tacgcgggag cctcgatgcc cctcctgctg ctcttcgtcc tctccgagca
     7441 gtcgctgggg accgtggcga acagcgaggt cgtggccacg gagatcgtcc gaacgctcgt
     7501 gggaagcatc gggctcgtgg ccagcgtccc catcacgacg tggctggcgt cgtgggtggt
     7561 gggcgcctcg ccggcgccgg aggcggcgtg agcgagctcc gcgttttcga cctctggggc
     7621 gtcccgttcg acggcggggc cacgcttggc tggccgggcg cccggaacgg gcccgaccgg
     7681 gtccgccgcg cgctgtcttg gatggcgatg cgcgaggagg aggggctggt ccgggcggtg
     7741 gaccccggcg aggacctgcc cgtccccgag gtggccgacc gaggcgacgt ggacgtcgtg
     7801 ccgcacgacc tgatggccac gatcgacgcg gtgtcggatg ccgtgacggg atccgtcaca
     7861 gcgagccgcg tgccgatcgt cgtggggggc gaggactcgc tcttcttccc ctcggtgcgg
     7921 gggctccacg acgcggtgga gggatcggtc ggcgtcatac acttcgacgc gcacttcgac
     7981 ctgctcgacg agaaccggag gcaggggcgg ttcagtcact ccagcgggat gcggcggagc
     8041 ctggagctcg agcgcgtggc ggccgggcac tgcgtccagg tcgggacgcg gaccttcaac
     8101 ttccccagct ccgcggcgtt cgtggagaag atcggcctgc tgcagatccc ggcggtcgag
     8161 gtgcacgagc agggggtgac cggcacggtc gaacgggtcc tggagcgcgt gggcggggtc
     8221 gatcacgtct tctggtcctt agacatcgac gtgatcgacc ccgcccacg
//
LOCUS       DASUTW010000275         5686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_869335, whole genome shotgun sequence.
ACCESSION   DASUTW010000275 DASUTW010000000
VERSION     DASUTW010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869335"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..338
                     /locus_tag="VF058_09265"
     CDS             18..338
                     /locus_tag="VF058_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018024577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX6208527.1"
                     /translation="MSIELTEAAAQKVRELLDQEGAEAALRVAVQPGGCSGLRYSMYL
                     DDELTDSDQIAEQYGVRVAVDRMSAPYLQGAKVDFVDSLEATGFTIDNPAAQGTCACG
                     HSFN"
     gene            557..1546
                     /locus_tag="VF058_09270"
     CDS             557..1546
                     /locus_tag="VF058_09270"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208528.1"
                     /translation="MAARVTVVGSYPKPPHEGEEFTLRRTLHALDRGDASVVDVRAAQ
                     DDLVREVIEEQARAGIELVTDGQVRWDDAQTRFAEGLEGFGTTGLLRYFDTNTYFRQP
                     VVKAEVARRGRIVLDEFLFARDHSPVPVKQALIGPYTLAALSVDEHYGHLRRLVEDLA
                     AALNEEARELAEAGCEVIAFDEPALARIPGSRPGDLAVLGDVADRLTAGVGATTVLQT
                     FFGDVAEHGPAFFDLPFDAFGLDLVAGPGNLEAVHEFPADRILQAGIVDSRNTRLEDP
                     DDLARRIEGLAATVDPERLWLSPSAGLEYLPREAAQRKCALLAEAARRFRGDR"
     gene            1543..2520
                     /locus_tag="VF058_09275"
     CDS             1543..2520
                     /locus_tag="VF058_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013987812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX6208529.1"
                     /translation="MSDVLLTTQVGSLPKPDYLLRARSAFARGEISREELRDLERRAT
                     REWVEFQNEIGIDVVVDGEQYRGDMVAFFAEELEGFSRGGLVRSYGNRYYRKPIITGP
                     IRRTHPITVEWWSYASSLTDRPVKGMLTGPYTIRDWSFDEHYDTRRDAVLALAEAIRD
                     EAVALQEAGAEYIQIDEPAVSTRMDELDLAIEALGVVTEPLSVHTITHICYGDFHNAY
                     PRMLDLPVDQIDLELTNSDFDLLDRFAEHPFTKSIGLGVVDVHSHRIETVDEVVKGIE
                     RTLEVIPPGRVYVDPDCGLKTRTVEEAKDKLRVVVEGARRVRSRLGIDP"
     gene            2589..3971
                     /locus_tag="VF058_09280"
     CDS             2589..3971
                     /locus_tag="VF058_09280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELWxxDGT repeat protein"
                     /protein_id="HEX6208530.1"
                     /translation="MPRHTIRTLLVVLALVGPTLVPGVAGAAPRVRLVKDIHPGGDSN
                     PGGFVSLGDRTLFSADDGVRGRELWRTDGTRRRTKRVKDIWPGAGGSAPRSLARMGKR
                     VLFVADDGVHGLELWRTNGRPTGTRLVKDINPGGSAFPAMGPIDLVGVGGTLFFAADD
                     GTRGRELWRSDGTRRGTKLVKNIWPGADDGSPGELTRFGKHLLFVANDGTRGRELWRS
                     DGTRRGTKLVKNIWPAGDSSLPNFITAFRRVAYFQANDGTRGRELWRTNGRPKGTRMV
                     KDIRPGPDGSSPEDFFMVGGRLIFEANDGTRGFEPWRSDGTRKGTKILKDVAPGMTSS
                     SPDYGFVFRGILYQTMSDGSRGSELWRTDGTRRGTKLVKDIYPGPDSSIPRYFVVAGR
                     KRLVFAATSATGQELWRTGGTRKGTKPTRDIWPGPTGSNPFGLYSTKSHVLFSADDGT
                     RGRELWAYRR"
     gene            4077..>5686
                     /locus_tag="VF058_09285"
     CDS             4077..>5686
                     /locus_tag="VF058_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX6208531.1"
                     /translation="MTKQVERKEDVVVRFAGDSGDGMQLTGDRFTNATAVIGNDLATL
                     PDFPAEIRAPAGTLHGVSAFQIHFSSHDILTPGDHPNVLVAMNPAALKANIGDLQAGG
                     TIIANENAFVDRNLEKAGYETNPLEDGSLADYQVFPVKMTDMTLEAVKGLEVTKKEGE
                     RAKNFFALGLVSWLYGRPTEITIGWIEEKFAKRPAIRDANLAAFRAGYNFGETAELFA
                     TSYTIERAPAEPGIYRNTNGTVALAWGLIAASVKSGLPMFYAGYPITPASELLHELSR
                     HKNFGVRTFQAEDEIAAANMALGAAFAGHLGVTGTSGPGLDLKAETISLAVTLELPMV
                     IVDVQRAGPATGMPTKTEAADLMLAMYGRHGESPLPIVAAYSPAQCFDAAFEAVRIAV
                     EYRTPVILLSDTFLGNSSEPWLIPDPDTLPQIDPRFATEANHDEGFMPYLRDEKLARP
                     WAVPGTPGLEHRIGGLEKVADSTGNISYDPENHERATHLRLQKVKNVADSLPPLEVDD
                     PDGDAELLVVGWGSTQGTIKAALAAVRDR"
BASE COUNT          921 a         1953 c         2035 g          777 t
ORIGIN      
        1 ggtataatcg tcaggtcatg tcgatcgagc tcacggaagc ggcggcgcag aaggtcaggg
       61 agctcctgga ccaggagggc gccgaggcgg ctctgcgcgt ggcggtccag cccggcgggt
      121 gctcggggct ccggtactcg atgtacctgg acgacgagct caccgactcg gaccagatcg
      181 ccgagcagta cggcgtccgc gtggccgtgg accggatgag cgccccgtac ctccaggggg
      241 cgaaggtcga cttcgtggac tccctcgagg ccacgggctt cacgatcgac aaccccgccg
      301 cccagggcac ctgcgcctgc ggccactcct tcaactaggc gggaaatcac tccttaccga
      361 gcggagacct ctgtgctagg gttgcgccca gcatcaggag ggttccggcg gatgccggag
      421 ctcggcaacc tgggaggacc cccaaggtgc catcccgcgc tcgcgcgggg atgcggtccc
      481 gatcggcgga ggccgggacg gggcaaccaa cggtccctct gcttgccgaa ccctccatga
      541 cgtggaagga gcacgcatgg ccgcgcgcgt cacggtggtc gggtcgtacc cgaaaccgcc
      601 gcacgaggga gaggagttca ccctccgcag gaccctgcac gcgctcgatc ggggcgacgc
      661 ctcggtggtc gacgtgcggg ccgcccagga cgatctcgtc cgcgaggtga tcgaggagca
      721 ggcgcgagcc gggatcgagc tcgtcaccga cgggcaggtc cgctgggacg acgcccagac
      781 ccggttcgcc gaggggctgg aggggttcgg cacgacggga ctcctccgct acttcgacac
      841 caacacctac ttccgccagc cggtggtcaa ggccgaggtg gcgaggaggg gccgtatcgt
      901 gctcgacgag ttcctcttcg cccgggacca cagccccgtc cccgtcaagc aggcgctgat
      961 cggcccgtac acgctggccg cgctcagcgt ggacgagcac tacgggcatc tgaggaggct
     1021 cgtggaggac ctggcggcgg cgctgaacga ggaggcgcgg gagctggccg aggccgggtg
     1081 cgaggtgatc gcgttcgacg agccggcgct ggcccggatc cccgggtcga ggcccgggga
     1141 cctggccgtg ctcggcgacg tcgccgatcg cctgaccgcg ggcgtgggcg cgacgaccgt
     1201 cctccagacg ttcttcggcg acgtcgccga gcacggcccc gccttcttcg accttccctt
     1261 cgacgcgttc ggcctcgacc tcgtggccgg accggggaac ctggaggcgg tccacgagtt
     1321 ccccgcggac cggatcctcc aggcggggat cgtggactct cgcaacactc ggctggagga
     1381 ccccgacgac ctggcccggc ggatcgaggg tctggccgcg acggtcgatc ccgagcggct
     1441 ctggctgagc ccctccgcgg ggctggagta tctgccgcgc gaggccgcgc agcggaagtg
     1501 cgcgctgctg gccgaggcgg cccgacggtt caggggggac cgatgagcga cgtgctgctg
     1561 acgacccagg tggggagcct ccccaagccc gactacctcc tccgggcccg ctcggcgttc
     1621 gcccggggcg agatctcccg ggaggagctg cgggatctcg agcgccgcgc cacccgcgag
     1681 tgggtggagt tccagaacga gatcgggatc gacgtggtcg tggacggcga gcagtaccgc
     1741 ggcgacatgg tggctttctt cgccgaggag ctcgaggggt tctcccgggg cgggctcgtc
     1801 cgctcctacg gcaaccgcta ctaccggaag ccgatcatca cgggcccgat ccggcgaacg
     1861 caccccatca ccgtggagtg gtggtcgtac gcgagctcgc tcaccgaccg cccggtgaag
     1921 ggcatgctga ccgggcccta cacgatccgg gactggtcgt tcgacgagca ctacgacacg
     1981 aggcgcgacg cggtgctggc gctggccgag gcgatcaggg acgaggccgt ggcgctgcag
     2041 gaggcggggg ccgaatacat ccagatcgac gagccggcgg tctccacgcg gatggacgag
     2101 ctcgacctgg ccatcgaggc gctcggcgtg gtgaccgagc cgctctcggt gcacacgatc
     2161 acccacatct gctacggcga cttccacaac gcctacccgc ggatgctcga cctgcccgtg
     2221 gaccagatcg acctggagct gaccaactcg gacttcgacc tgctggatcg cttcgcggag
     2281 cacccgttca cgaagtcgat cgggctgggc gtggtggacg tccactcgca ccggatcgag
     2341 accgtggacg aggtcgtgaa ggggatcgag cggaccctcg aggtgatccc cccggggcgc
     2401 gtctacgtcg atcccgactg cggcctgaag acccggacgg tcgaggaggc gaaggacaag
     2461 ctccgcgtgg tggtcgaggg cgcccggcgg gtccggagca ggctcggcat cgacccctag
     2521 ccttccgggg cccgatgccg tacgatgcgg ccctccggtc ggcgttccaa gcgagaggga
     2581 ggctcaccgt gcccaggcac accatccgca ccttgctcgt cgtcctcgcc ctcgtcggac
     2641 ccacgctcgt ccccggcgtc gcgggcgccg ctccgcgcgt ccggctcgtc aaggacatcc
     2701 atccgggagg cgactccaac cccggcggct tcgtctccct gggggatcgc acgctcttct
     2761 cggcggacga cggcgtccgc ggccgcgagc tctggaggac cgacgggacc cggaggcgca
     2821 cgaagcgcgt gaaggacatc tggcccggcg cgggcggatc ggctccacgg tccctggccc
     2881 gcatggggaa gcgggtcttg ttcgtggcgg acgatggcgt ccacggattg gagctctgga
     2941 ggacgaacgg gaggccgacg ggtacgcggc tggtgaagga catcaaccca gggggatccg
     3001 ccttccccgc catggggccg atcgatctgg ttggcgtggg cggaacgctg ttcttcgccg
     3061 ccgacgacgg gacgcggggc cgcgagctgt ggaggtcgga cgggacccgc cggggcacga
     3121 agctggtcaa gaacatctgg ccgggcgcgg acgacggctc gccgggggag ctgacgcggt
     3181 tcgggaagca cctcctgttc gtggccaacg acggcacccg aggccgagag ctgtggaggt
     3241 cggacgggac ccgccggggc acgaagctgg tcaagaacat ctggccggcg ggggactcga
     3301 gcctcccgaa cttcatcacc gccttcagga gggtcgccta cttccaggcc aacgacggca
     3361 cccgaggtcg ggagctgtgg cgcacgaacg gcaggcccaa gggcacgagg atggtcaagg
     3421 acatcaggcc gggcccggac ggcagctccc ccgaggactt cttcatggtg ggaggtcgcc
     3481 tgatcttcga ggcgaacgac ggtactcggg gcttcgagcc ctggcgctcg gacggcacgc
     3541 ggaaggggac gaagatcctg aaggacgtcg cgccggggat gacctcgagc agccccgact
     3601 acggcttcgt cttccgaggg atcctgtacc agacgatgtc cgacgggagc cgtggatcgg
     3661 agctgtggcg gaccgacggc acccgccggg gcacgaagct ggtcaaggac atctatccgg
     3721 ggcccgactc gagcatcccg aggtacttcg tggtggcggg gaggaagcgg ctggtcttcg
     3781 cggccacgtc ggccacgggg caggagctct ggaggacggg cggcacgcgg aaggggacga
     3841 agccgacccg ggacatctgg cccggtccca ccggctccaa cccgttcggc ctctactcga
     3901 cgaagtcgca cgtgctcttc tcggcggacg acggcacccg cggccgcgag ctgtgggcgt
     3961 accgtcggtg accgaggccg cttcgggacg accgaccacg cgttcgccca cgcggtaaca
     4021 tcggagacga gcccgatccg agaggagagg cggccggtcg gcgccgacac gatgcgatga
     4081 cgaagcaggt cgagcggaag gaagacgtgg tcgtccggtt cgccggtgac tcgggcgacg
     4141 gcatgcagct caccggggac cgcttcacga acgcgaccgc cgtgatcggg aacgacctgg
     4201 ccaccctccc ggacttcccc gcggagatcc gcgccccggc cggcaccctt cacggggtct
     4261 cggccttcca gatccacttc tcgtcacacg acatcctcac gcccggggat cacccgaacg
     4321 tcctcgtggc gatgaacccc gcggccctga aggccaatat cggcgacctc caggccggcg
     4381 ggacgatcat cgccaacgag aacgctttcg tggaccgcaa cctcgagaag gcaggctacg
     4441 agacgaaccc gctcgaggac ggctcgctcg ccgactacca ggtcttcccc gtgaagatga
     4501 ccgacatgac cctggaggcc gtgaaggggc tcgaggtcac gaagaaggag ggggagcggg
     4561 ccaagaactt cttcgccctg ggcctcgtct cgtggctcta cgggcgtccc accgagatca
     4621 cgatcggctg gatcgaggag aagttcgcca agcggcccgc gatccgcgac gcgaacctgg
     4681 cggcgttcag ggccggctac aacttcggcg agaccgcgga gctgttcgcg acctcttaca
     4741 cgatcgagcg ggcgcccgcg gagcccggga tctaccgcaa cacgaacggc acggtcgccc
     4801 tggcctgggg cctgatcgcg gccagcgtca agagcggtct cccgatgttc tacgcgggct
     4861 acccgatcac ccccgcctcc gaactgctcc acgagctctc ccgccacaag aacttcgggg
     4921 tgcgcacgtt ccaggccgag gacgagatcg cggccgcgaa catggcgctc ggcgcggcgt
     4981 tcgcggggca cctcggcgtc accggcacga gcggtcccgg gctcgacctc aaggccgaga
     5041 cgatctcgct ggccgtcacg ctggagctgc ccatggtgat cgtcgacgtc cagcgggcgg
     5101 gccccgccac cgggatgccc acgaagaccg aagccgccga cctgatgctc gcgatgtacg
     5161 ggcggcacgg agagtcgccc ctccccatcg tggcggccta ctcgccggcc cagtgcttcg
     5221 acgccgcctt cgaggccgtg cgcatcgcgg tcgagtaccg gacgccggtg atcctgctct
     5281 cggacacgtt cctggggaac tcctcggagc cgtggctgat ccccgacccc gacaccctcc
     5341 cgcagatcga cccgaggttc gccaccgagg cgaaccacga cgagggattc atgccgtacc
     5401 tgcgcgacga gaagctcgcg aggccctggg ccgtccccgg gacgcccggc ctcgagcacc
     5461 gcatcggggg gctggagaag gtggccgact ccaccgggaa catctcctac gaccccgaga
     5521 accacgagcg cgccacgcac ctgcggctgc agaaggtgaa gaacgtcgcc gactcgctgc
     5581 cgccgctcga ggtcgacgat cccgatggcg acgcggagct gctcgtcgtc ggctggggat
     5641 ccacgcaggg gacgatcaag gcggccctcg cggccgtccg cgatcg
//
LOCUS       DASUTW010000276         5070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_351059, whole genome shotgun sequence.
ACCESSION   DASUTW010000276 DASUTW010000000
VERSION     DASUTW010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351059"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1434
                     /locus_tag="VF058_09290"
     CDS             172..1434
                     /locus_tag="VF058_09290"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX6208532.1"
                     /translation="MIGRTEYASGLRVITERMPGVRSVTLGVWVPVGSRDEPARTAGA
                     SHFLEHLLFKGTKARSARDIAEAFDAVGGELNAFTGREQTVFYARVLDKDLPMAVDHL
                     TDMIERSLLRSRDVEAERQVILEEIHMTEDAPDQLVHDDFARTLWGGHPLGRAILGTR
                     ETIEGMGRDQIRRFWKRHYVPGNLVFVAAGNVEHEALTGLIESLVETGAPVSGRRSTV
                     RDGGTPPRPSGSWEVRRRRTEQAHIAYGTAGLARRDPRRFAFGVVNNALGEGMSSRLF
                     QEVREKRGLAYSVYSYHAMYAETGLFCVYAGTTPVRATEVLRVIRGQIEDLAEKGLGP
                     DELERAKGHMKGGLVLSQEDTSSRMNRLGKSEVAHGEILTVDEVIERIDTVTAEDARD
                     VAADVLGREPSLAVIGPLGKDALREVLT"
     gene            1434..2219
                     /gene="dapB"
                     /locus_tag="VF058_09295"
     CDS             1434..2219
                     /gene="dapB"
                     /locus_tag="VF058_09295"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX6208533.1"
                     /translation="MRVGVIGACGKMGAMTSRAVVDDPELSLVAAVHPSRAGEDLAVA
                     VGHPRAEGRVSAQLEALVQADAEVAVDFTTPDVVMDDVRFGCTHGIHMVVGTTGVGPD
                     EQATIREWLKDSDANVVVAPNFSIGAVVAQRLAEEAARHFPAAEIIELHHDAKVDAPS
                     GTAAATARRIADARGEAWKGPDAETIEGVRGGSVDGIPVHSVRLPGLVAHQEVLFGGR
                     GQTLSIRHDSTDRSSFMPGVVLAIKEVARRPGLTVGLESLLEL"
     gene            complement(2216..2638)
                     /locus_tag="VF058_09300"
     CDS             complement(2216..2638)
                     /locus_tag="VF058_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208534.1"
                     /translation="MRRYLVVANQTLGGAHLVRRLRAAAARGPCSFFFLVPATPPQDH
                     ATWTEGEAEALAGERLARALIRARELGVEVDGDVGDPDPVQAIADAMIDDRYDEIIVS
                     TLPPGISRWLKLDLPHRVERRFDVPVTHVVGEPEPARG"
     gene            complement(2676..3206)
                     /locus_tag="VF058_09305"
     CDS             complement(2676..3206)
                     /locus_tag="VF058_09305"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HEX6208535.1"
                     /translation="MRYLELRRHADNEGDALTEAGKATAERVGRSMEGPFDGVFTSPA
                     KRAATTAAWFLRGMGQQLPQLHGVTEGLTSPEEKRWREAGERTGTWKVDDIEREDPEL
                     LRGELPRLADAILQMFAAIQDGQRALVVGHSPFLEAAVYALTGVVPEPLGKCEGILLA
                     HDGERVTVEGDLRLPG"
     gene            complement(3206..3520)
                     /locus_tag="VF058_09310"
     CDS             complement(3206..3520)
                     /locus_tag="VF058_09310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HEX6208536.1"
                     /translation="MKAHIWLRKKDGMSDEEFKDHWLTKHAPIAGDGYEHLTGYTVAL
                     VTRVPEGQERPYDGVAILEWADRDGFKADIASPVAKESTDDLANFTASFGLLFIEEYT
                     VK"
     gene            complement(3585..>5070)
                     /locus_tag="VF058_09315"
     CDS             complement(3585..>5070)
                     /locus_tag="VF058_09315"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEX6208537.1"
                     /translation="AIVSSNVDLPIDYCDGGSDPTTIRVPAGRFRLSVGPAGDNAGLT
                     GDLDITDDVVIRGAGMGRTIIDANGLDRVFDVDTGFAGILVVLRDLTITGGSVTGTSG
                     GGIWNNGILVLKNVEVRGNESLDAVTGGGGGIWNYDTGILSVVESRIVGNRTTGSGTG
                     GGLRNRGTASVFDTLIKGNRSRDGGGIMSFPTPATLTVRDSALVGNIAFDGDGGGFNN
                     NNGATATFENVTVSKNRAARFGGGIYHFSGSSSFSHLTVADNRADFDGNAVGDGGGLA
                     VSIGTVELRASAFARNKVGSGAAASDCSGGAQIQTLGGNIERGPNECNLLPLSGDVLA
                     NPKLGKLRNLGGPTPTHALAQKSKARGLVSASDCAPTDQRGVPRRGRCDAGAYELAAC
                     AGVVVNRVGTKGRDVLRGTRGPDGFLALAGNDVVRGRGGGDAMCLGGGRDRAFGGAGN
                     DVLRGHGGNDVLKGQAGNDALIGGPGRDRCVQGSGTGRLRGCER"
BASE COUNT          681 a         1823 c         1837 g          729 t
ORIGIN      
        1 gaggagggcg gctcggagga ggcctcggcc gacgaaggcc gacgcgacga ccgcccgcgg
       61 gaggagcgtc cgcgtgagga gcgcggcgag cgtggtgggg gcggcggcgg cgagcgccgc
      121 aggcgccgcc gccgccgccc cgagggcgac cgcggggagt agcccaccca ggtgatcggc
      181 cgcaccgagt acgcgtccgg gctccgggtc atcaccgagc ggatgcccgg ggtccggtcg
      241 gtcacgctcg gcgtctgggt cccggtgggt tcgcgcgacg agccggcccg caccgccggg
      301 gcctcgcact tcctggagca cctcctcttc aaggggacca aggcgcgctc ggcccgggac
      361 atcgccgagg ccttcgacgc ggtgggggga gaactcaacg ccttcaccgg gcgggagcag
      421 acggtgttct acgcacgggt gctggacaag gacctcccga tggcggtgga ccatctcacc
      481 gacatgatcg agcgctcgct cctgcgctcg cgcgacgtgg aggccgagcg gcaggtgatc
      541 ctcgaggaga tccatatgac cgaggacgcc cccgatcagc tcgtccacga cgacttcgcc
      601 cggaccctgt ggggcggcca cccgctgggg cgcgcgatcc tgggcacccg ggagacgatc
      661 gaggggatgg ggcgcgacca gatccgcagg ttctggaagc gccactacgt ccccggcaac
      721 ctcgtgttcg tcgcggcggg gaacgtggag cacgaggcgc tgaccgggct catcgagtcg
      781 ctcgtggaga cgggcgcacc cgtctcgggg cggcgctcga ccgtccgcga cggcgggacc
      841 cctccccggc cctccggcag ctgggaggtt cgccggcgca ggaccgagca ggcgcacatc
      901 gcctacggca ccgcgggcct ggcccgccgc gacccccgcc gcttcgcctt cggcgtcgtg
      961 aacaacgcgc tgggggaggg gatgtcgtcc cggctgttcc aggaggtgcg cgagaagcgg
     1021 ggcctggcct actccgtcta ctcgtaccac gcgatgtacg cggagaccgg gctcttctgc
     1081 gtctacgctg ggacgacccc ggtcagggcc acggaggtcc tgcgggtgat ccggggccag
     1141 atcgaggacc tggccgagaa ggggctgggg ccggacgagc tggagcgggc caaaggccac
     1201 atgaagggcg ggctcgtcct ctcccaggag gacacctcct cgcggatgaa ccgcctgggg
     1261 aagtcggagg tggcgcacgg ggagatcctc accgtggacg aggtgatcga gcggatcgat
     1321 accgtgacgg ccgaggacgc cagggacgtg gccgccgacg tgctcgggcg cgagccctcg
     1381 ctggccgtca tcgggccgct gggcaaggac gccctccggg aggtgctgac gtgatccggg
     1441 tgggcgtgat cggggcctgc ggcaagatgg gggcgatgac cagccgggcc gtggtcgacg
     1501 atccggagct ctcgctcgtc gcggcggtcc atccgtcgcg ggccggcgag gacctcgccg
     1561 tcgcggtggg gcacccccgg gcggagggca gggtctccgc gcagctcgag gccctcgtgc
     1621 aggccgacgc cgaggtcgcg gtggacttca ccacgcccga cgtcgtgatg gacgacgtcc
     1681 gcttcggatg cacccacggg atccacatgg tggtggggac cacgggcgtg gggcccgacg
     1741 agcaggccac gatccgggag tggctgaagg actcggacgc gaacgtcgtc gtggcgccga
     1801 acttctcgat cggcgctgtg gtggcgcagc gactggccga ggaggcggcg cggcacttcc
     1861 cggcggccga gatcatcgag ctccatcacg acgccaaggt cgacgcgccg tcgggcaccg
     1921 cggccgccac ggcccggcgg atcgccgacg cgcgcggcga ggcgtggaag ggccccgatg
     1981 ctgagacgat cgagggcgtc cgcggcggat ccgtcgacgg catcccggtc cacagcgtgc
     2041 gactcccggg cctcgtcgcc catcaggagg tcctcttcgg cggacggggg cagaccctct
     2101 cgatccggca cgactcgacc gaccgctcgt cgttcatgcc cggggtggtg ctggcgatca
     2161 aggaggtcgc tcgccgtccg ggcctgacgg tcggcctcga atccctcctg gagctctagc
     2221 cccgcgccgg ctcgggctcg ccgaccacgt gggtcacggg cacgtcgaac cgtcgctcca
     2281 cgcggtgcgg caggtcgagc ttgagccagc gcgagatccc tggtggcagc gtggacacga
     2341 tgatctcgtc gtacctgtcg tcgatcatcg cgtcggcgat cgcctggacg gggtcggggt
     2401 cgcccacgtc tccgtccacc tccacgccga gctcccgggc ccggatcagc gcccgggcca
     2461 gccgctcacc ggccagcgcc tccgcctcgc cctcggtcca agtggcgtga tcctggggcg
     2521 gagtggccgg aacgaggaag aagaacgagc agggcccccg ggccgcggcg gctcgcagac
     2581 gccggaccag gtgcgcaccg ccgagcgtct ggttggcgac gaccaggtag cgacgcatcg
     2641 gcatcacctc ctcgccccat cgtaggagat cgcgctcagc ccgggagccg gaggtcgccc
     2701 tccacggtga cgcgctcgcc gtcgtgggcg agcaggatgc cctcgcactt ccccaggggc
     2761 tccggcacca ccccagtcag cgcgtagacg gcggcctcga ggaatgggct gtgtcccacc
     2821 acgagcgccc gctgcccgtc ctggatcgct gcgaacatct ggaggatggc gtcggccagc
     2881 ctgggcagct cgccgcgcag cagctcgggg tcctcccgct cgatatcgtc caccttccag
     2941 gtcccggtgc gctccccggc ctcccgccag cgcttctcct cgggggacgt gagcccctcg
     3001 gtgacgccgt ggagctgggg gagctgctgg cccatcccgc ggaggaacca ggcggcggtc
     3061 gtggcggcgc gcttcgcggg cgaggtgaac accccgtcga agggaccctc catcgagcgg
     3121 cccacgcgct cggccgtggc cttccccgcc tcggtgaggg cgtcgccctc gttgtcggcg
     3181 tgccggcgaa gctcgaggta ccgcactact tgaccgtgta ctcctcgatg aagaggagac
     3241 cgaaggaggc ggtgaagttg gccaggtcgt cggtgctctc cttggccacc gggctcgcta
     3301 tgtcggcctt gaacccgtcg cggtccgccc actcgaggat cgccacgccg tcgtacggcc
     3361 tctcctgtcc ctcggggacc ctggtgacga gggccaccgt gtagccggtc aggtgctcgt
     3421 agccgtcgcc ggcgatcggg gcgtgcttcg tcagccagtg gtccttgaac tcctcgtcgg
     3481 acatgccgtc cttcttgcga agccagatgt gagccttcac ggcggcctcc ttcgggatgc
     3541 ggtcaccgca tcctatccgg cggcggaggg cggtgggcga ccgcctaccg ctcgcagccg
     3601 cgaagcctcc cggtgcccga cccctggacg cagcggtccc gtccaggacc gccgatcagc
     3661 gcgtcgttcc cggcctgccc cttgagcacg tcgttgccac cgtgcccccg aagaacatcg
     3721 ttcccggccc cgccgaacgc cctgtcccgg ccccccccga ggcacatggc gtcgccgccg
     3781 ccccgtcccc ggacgacgtc gttgcccgcg agcgcgagga acccatcggg gccccgcgtc
     3841 ccccggagca cgtcgcgccc cttcgtgccg acccggttga ccacgactcc cgcgcaggcc
     3901 gcgagctcgt aggctcccgc gtcgcagcgg cccctgcgcg ggacgccgcg ctggtccgtc
     3961 ggggcgcagt cggaggcgga cacgagaccg cgcgccttgc tcttctgggc cagcgcgtgg
     4021 gtcggcgtgg ggccgccgag gttgcgcagc ttcccgagct tcgggttggc gaggacgtcc
     4081 cccgacaggg ggaggaggtt gcactcgttg gggcctcgct cgatgttccc cccgagcgtc
     4141 tggatctggg cccctccgga gcagtcggac gcggcggcgc cgctgcccac cttgttgcgg
     4201 gcgaacgcgg aggctcgcag ctccacggtc ccgatggaga cggcgaggcc gccgccgtca
     4261 cccaccgcgt tgccgtcgaa atcggcccgg ttgtccgcca ccgtgaggtg cgagaaggag
     4321 gacgagccgc tgaagtggta gatgccgccc ccgaaccgcg ccgctcgatt cttgctcacg
     4381 gtcacgttct cgaacgtggc ggtggccccg ttgttgttgt tgaacccgcc gccgtccccg
     4441 tcgaaggcga tgttgccgac gagcgcgctg tcccgcaccg tgagcgtcgc cggcgtcggg
     4501 aagctcatga tgccgccccc gtcccggctc cggttcccct tgatcagggt gtcgaacacc
     4561 gacgcggttc cacggttccg taggccccct ccggtgccgg agccggtggt ccgattgccc
     4621 acgatgcggc tctcgacgac ggagaggatc cccgtgtcgt agttccagat cccaccgcct
     4681 cctcccgtca ccgcgtccag cgactcgttg ccccgtacct cgacgttctt cagcacgagg
     4741 atgccgttgt tccagatccc tccgcccgag gtgccggtga cggagccgcc cgtgatcgtg
     4801 aggtcgcgga ggacgacgag gatcccggcg aaccccgtat ccacgtcgaa gacgcgatcc
     4861 aggccgttcg cgtcgatgat ggtccgtccc atccccgccc cgcggatcac gacgtcgtcg
     4921 gtgatgtcga ggtcgcccgt gagcccggcg ttgtcgccgg cggggcccac cgacagcctg
     4981 aagcgcccgg ccgggacgcg gatcgtcgtg gggtcgcttc ctccgtcgca gtagtcgatc
     5041 gggaggtcca cgttcgacga cacgatcgcc
//
LOCUS       DASUTW010000277         6246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_869877, whole genome shotgun sequence.
ACCESSION   DASUTW010000277 DASUTW010000000
VERSION     DASUTW010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869877"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VF058_09320"
     CDS             complement(<1..760)
                     /locus_tag="VF058_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6208538.1"
                     /translation="METRAFGGTGLELPVIGFGTWNVFDVGPQGEARAAEVVAAGLAA
                     GVRVFDSSPMYGRAEEVLGRALGDRRGDAVIATKIWTPSLEEGQRQFASQLDFYEGRV
                     ELLQVHNLVAWEQHLGWMERERDQGRIGHLGATHYSPRAYDELETVMRSGRISFVQIP
                     LSPWEREAEERILPLAEELGLGVIVMRPRGSGKAGRGPDPEELAPLRELGIETWAQAL
                     LKWVLSDPRVHLPIPATSSPDHARENAAAGEPPFL"
     gene            complement(760..1602)
                     /locus_tag="VF058_09325"
     CDS             complement(760..1602)
                     /locus_tag="VF058_09325"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX6208539.1"
                     /translation="MSFLIVSNPEAGSAEEGMADRALHLLPDAEAFHLEPDADLGRAV
                     DEAVRDDRVVVAAGGDGTVSACVQHLVGRGTLGVLPAGTRNHLARDLDLNDEEAALAA
                     LKERRTSRVDLGRLDDRHFINNVALGLYPQLVEKRERDEERIGRWPAMLRAASRVLRE
                     GEPVVGTIAADGDARKVGAWMVFVGNNRFLTTPGRIGRRERLDEGVLDLRLLLVGTRA
                     RLAYSVVRGRTWGPRRVVRREARRVELRLLQEGALAVDGEVEEPVRRAEIEIVPRALT
                     LVVP"
     gene            complement(1612..2901)
                     /locus_tag="VF058_09330"
     CDS             complement(1612..2901)
                     /locus_tag="VF058_09330"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6208540.1"
                     /translation="MARLAVIGGGVAGLAAARAGVRAGWDVILLEASQRLGGKVLTEE
                     VDGVPWEWGPDAIVATKPRGEDLLEELGLLEDAVPPATGRAYLLVGGELRPIPPGLAM
                     GFPKGPGALMEAARSGILTRAEALRGAVEGAMPGRPRGSVAEVARARLGRGVAEKLTL
                     PLVSAVFGTADQDLKAAIPEMAEARSLTAGAARRPSVRFLGLRGGMGRLAEALVEEGV
                     EVRTGVRAEAIEGSAGAYVVRADGAGVEADAVVVAVPAPSARPLLPSLPEGLGDYTSS
                     AVIHLRYPEGALGRPLDAAGYVVADPGIVQACSWVSSKWPHLRESGPHLRAVVVDPEA
                     EDDEALAAAVVEEVGRVMEAGADPHVVRVRRWPVALPRYAPGHLETVRRTREALPPSI
                     QLAGASFDGVGVPDCVAGGEEAFRRIASLWETTPGAG"
     gene            complement(2903..3691)
                     /locus_tag="VF058_09335"
     CDS             complement(2903..3691)
                     /locus_tag="VF058_09335"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017574098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX6208541.1"
                     /translation="MSKRSRQRQLQKLAERRAEERRRRRRRRIAAGVVGGAIALGGLT
                     VGALALFGGEEPREEPVAGPTPTPTQEETMTEPTVACGGEVPEAADQRKPTYEQAPEM
                     QLQDGADYRAVLKTSCGRIEIDLFEGQTPISVNNFVFLAREGFYDGTVFHRVIADFMN
                     QGGDPEGTGMGGPGYEFEDEIVADLTFDRPGLLAMANAGPGTNGSQFFITAAPTPHLD
                     GAHTIFGEVTGGMDVVNQINGLPTDPSDKPVDTVYIESIEIREA"
     gene            complement(3703..5097)
                     /locus_tag="VF058_09340"
     CDS             complement(3703..5097)
                     /locus_tag="VF058_09340"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HEX6208542.1"
                     /translation="MTRSSDLQAVAEQLGREPTTQFEVVARCPGGRHPLVIRNHPVDR
                     DGNPFPTLYWLTCPEAVAGVSRLESEGWIARLDERVEEDPECRERFEDAALDYAAERE
                     RLFPGAGALSGVGGSGGGIKCLHAHYAHHLAGGANPIGAWVAERVEPIHEEPRGRVAA
                     VDMGTNSIRLLVADPKDDDVVEISRDLVITRLGQDVDRTKRLDPVALDRTIRVLERYA
                     RRARALRAYRIRVGATSAVRDASNRERLFAEVARVAGASAEVLTGEREAELSFLGATA
                     DAPAQPPYLVFDIGGGSTEFAFGTERPEASVSLQLGSVRTHERVKPEDLPTAEELAAM
                     DRVVEEALAEVRDAIPSGAATLVGVAGTTTTVQGIALGLDSYDPEALHGSVLSLDQAE
                     RVSERLASMTVAERAAIPVMTRGREDVIVAGSRILLGILRTFGFDDCLVSERDILDGL
                     AIEMVRSRNGDPTR"
     gene            complement(5102..5497)
                     /locus_tag="VF058_09345"
     CDS             complement(5102..5497)
                     /locus_tag="VF058_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX6208543.1"
                     /translation="MQPPFRKGAGPIADLEIGAVVTGTVQRITPYGAFIELEGGKVGL
                     VHISEIDRNYVRDVRDHLRETDEVEAKVVAIKDDGKIDLSIKALQDPPPKRLRRGQDP
                     GLEQKLKRFMRQSEERQVDVRKSTRDKRR"
     gene            5777..>6246
                     /locus_tag="VF058_09350"
     CDS             5777..>6246
                     /locus_tag="VF058_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208544.1"
                     /translation="MRKARRSHRGPGDSVRAEPEVRPFDESAAAWLIGEGVRYLNSAD
                     KEGELAYRRVTELLKDRNDTVEILTTILRQAGGGDAPLRWNLFYLLGDVGDAGAADLL
                     AEASLEALPDESSEDACEGPRDQELLVRTMAVHALGRVAGRHPEAGEQLLRIIG"
BASE COUNT          842 a         2440 c         2078 g          886 t
ORIGIN      
        1 cgaggaaggg cggctcgccg gccgccgcgt tctcccgggc gtggtcgggg ctcgaggtgg
       61 ccgggatcgg caggtgcacc cgcgggtccg acagcaccca cttcagcagg gcctgggccc
      121 aggtctcgat ccccagctcc cggagcggcg ccagctcctc gggatccggc ccccgccccg
      181 ccttccccga cccccgcggc cgcatcacga tcaccccgag cccgagctcc tcggccagcg
      241 gcaggatccg ctcctcagcc tcccgctccc acgggctcag cgggatctgc acgaaggaga
      301 tccgccccga ccgcatcacc gtctccagct cgtcgtaggc ccgcgggctg tagtgggtcg
      361 cccccaggtg cccgatccgc ccctggtccc gctcccgctc catccagccc aggtgctgct
      421 cccacgccac gaggttgtgg acctggagca gctccacgcg gccctcgtag aagtcgagct
      481 gactcgcgaa ctgccgctgt ccctcctcga gcgacggggt ccagatcttc gtggcgatca
      541 ccgcatcgcc ccgccggtcc cccagcgccc ggcccagcac ctcctcggcc cgcccgtaca
      601 tcggcgagga gtcgaacacg cgcacgccgg ccgccagccc cgccgccacg acctcggccg
      661 cgcgcgcctc cccctgagga cccacgtcga acacgttcca ggtcccgaag ccgatcacgg
      721 gcagctcgag ccccgtcccc ccgaacgccc tcgtctccat cagggcacca ccagcgtcag
      781 agctcgcggc acgatctcga tctcggccct ccgcaccggc tcctccacct cgccgtccac
      841 ggccaacgcg ccctcctgca ggaggcgcag ctcgacgcgc cgggcctcgc ggcgcacgac
      901 cctccgaggg ccccaggtgc gaccccgaac cacgctgtag gcgagacggg cccgggtccc
      961 cacgaggagc agccggaggt ccagcacgcc ctcgtccagg cgctccctcc gcccgatccg
     1021 gccgggggtc gtcaggaacc gattgttgcc cacgaacacc atccacgcac cgaccttccg
     1081 cgcatcgccg tcggcggcga tcgtccccac cacgggctcc ccctcgcgaa gcacccggga
     1141 cgcggctcgc agcatcgccg gccatcggcc gatccgctcc tcgtcacgct cccgcttctc
     1201 cacgagctgc ggatacagtc cgagggcgac gttgttgatg aagtggcggt cgtcgagccg
     1261 gcccaggtcc acccgcgagg tcctgcgctc cttcagcgcg gccagcgcgg cctcctcgtc
     1321 gttcaggtcg aggtcccggg ccaggtggtt gcgcgtgccg gcgggcagca cgccgagcgt
     1381 ccctcggccg acgaggtgct gaacgcaggc gctgacggtg ccgtccccgc ccgccgccac
     1441 caccacgcga tcgtcccgca cggcctcgtc caccgcccga cccaggtcgg catccggctc
     1501 caggtggaag gcctcggcgt cggggagcag gtgcagggcg cgatcggcca tgccctcctc
     1561 ggcagacccg gcctccgggt tgctcacgat gaggaagctc accgtggggt cctaccccgc
     1621 accgggcgtc gtctcccaca gagaggcgat cctccggaac gcctcctccc cgccggccac
     1681 gcagtccggg acgcccacgc cgtcgaagga agctcccgcg agctggatgc tgggcggcag
     1741 ggcttctcgg gtcctccgga cggtctccag atgccccgga gcgtaccggg ggagggcgac
     1801 gggccaccgg cggacgcgga ccacgtgcgg atcggctccg gcctccatca cgcggcccac
     1861 ctcctccacg acggccgcgg ccagggcctc gtcgtcctcg gcctccgggt ccaccacgac
     1921 ggcccgcagg tggggccccg actcccgcag gtgaggccac ttcgacgaga cccaggagca
     1981 ggcctgcacg atccccgggt ccgcgacgac gtaccccgcg gcgtcgagcg gccgacccag
     2041 cgccccctcc gggtagcgga ggtggatcac ggccgaggac gtgtagtcgc cgaggccttc
     2101 cgggagcgag gggaggaggg ggcgcgccga cggcgccggc acggccacca cgacggcatc
     2161 ggcctccacg ccggcgccgt cggcgcgcac cacgtaggcg cccgccgaac cctcgatcgc
     2221 ctccgcgcgg acgccggtcc ggacctcgac cccctcttcg accagcgcct cggccagccg
     2281 ccccatcccg ccgcgcagcc ccaggaaccg caccgaaggc cgtcgcgccg ccccggccgt
     2341 gaggctccgc gcctccgcca tctcggggat cgccgccttt agatcctggt cggcggtccc
     2401 gaagaccgcc gacaccagcg gcagggtgag cttctcggcc acgccccggc ccagcctggc
     2461 ccgcgccacc tccgcgacgc tgcccctcgg acggcccggc atcgcgccct ccaccgcgcc
     2521 ccgcagggcc tcggcccgcg tcaggatccc cgagcgggcc gcctccatca gcgcccccgg
     2581 ccccttaggg aaccccatgg ccaggccggg cgggatcggc cgcagctcgc ctccgaccag
     2641 caggtacgcg cggccggttg ccggcggcac cgcgtcctcg agcagcccga gctcctcgag
     2701 gaggtcctcg ccccggggct tcgtggccac gatcgcgtcg ggcccccact cccagggcac
     2761 cccgtcgacc tcctcggtga gcaccttgcc ccccaggcgc tgggaggcct ccagaaggat
     2821 aacgtcccat cccgcccgga cgccggcgcg ggccgcggcg aggcccgcga ccccgccccc
     2881 gatcaccgcg aggcgcgcca cctcacgctt cgcggatctc gatcgactcg atgtagacgg
     2941 tgtcgacggg cttgtcgctg gggtcggtgg ggagcccgtt gatctggttc accacgtcca
     3001 tgccgcccgt gacctcgccg aagatcgtgt gcgccccgtc gaggtgcggc gtgggggcgg
     3061 ctgtgatgaa gaactggctg ccgttcgtcc ccgggccggc gttggccatc gccagcagcc
     3121 ccggacgatc gaaggtcagg tcggccacga tctcgtcctc gaactcatag cccgggcccc
     3181 ccatgcccgt cccctcggga tcgccgccct ggttcatgaa gtcggcgatc acccgatgga
     3241 agaccgtgcc gtcgtagaac ccctctcgag cgaggaacac gaagttgttc acgctgatcg
     3301 gggtctggcc ctcgaacagg tcgatctcga tccgcccgca ggaggtcttc agcaccgccc
     3361 ggtagtcggc gccgtcctgg agctgcatct ccggggcctg ctcgtaggtc ggcttcctct
     3421 ggtcggcggc ctcggggacc tcgcccccgc aggccacggt cggctcggtc atcgtctcct
     3481 cctgggtcgg ggtcggcgtg gggccggcca cgggctcctc ccgcggctcc tccccaccga
     3541 acagcgcgag agcacccacg gtgaggccgc cgagcgcgat cgcgcccccc acgacccccg
     3601 ccgcgatccg cctgcgacgc ctccggcggc gctcctcggc gcgtcgctca gcgagcttct
     3661 ggagctggcg ctgcctcgat cgcttgctca tcgggagaga ggttagcggg tggggtcgcc
     3721 gttccgcgag cgaaccatct cgatcgccag accgtcgagg atgtcgcgct cggagacgag
     3781 gcagtcgtcg aagccgaacg tccggaggat gccgaggagg atgcgggagc cggccacgat
     3841 cacgtcctcg cggccccggg tcatcacggg gatcgccgcc cgctcggcca cagtcatcga
     3901 ggcgagccgc tccgagacgc gctcggcctg atccagggac agcacgctgc cgtgcagcgc
     3961 ctcggggtcg tacgagtcga gccccagcgc gatcccctgc acggtggtgg tcgtcccggc
     4021 cacccccacc agcgtggccg cccccgaggg gatcgcgtcc ctcacttcgg cgagcgcctc
     4081 ctccaccacg cggtccatcg ccgccagctc ctcggcggtc gggagatcct ccggcttcac
     4141 ccgctcgtgg gtgcgcacgc tccccagttg gagcgagacg gatgcctccg gcctctcggt
     4201 cccgaaggcg aactctgtgc tgccgccccc gatgtcgaag acgaggtagg gaggttgggc
     4261 cggcgcgtcg gccgtggccc ccaggaagga gagctcggcc tcacgctccc cggtgaggac
     4321 ctcggccgac gcgccggcca cccgcgccac ctccgcgaag agccgctccc tgttcgaggc
     4381 gtcccgcacc gcgctcgtgg ccccgacccg gatgcggtac gcgcggaggg ctctcgccct
     4441 ccgcgcgtac cgctccagca cccggatcgt ccggtcgagg gcgactggat cgagccgctt
     4501 cgtccggtcc acgtcctgtc ccagcctcgt gatcacgagg tcccgcgaga tctccaccac
     4561 gtcgtcgtcc ttcgggtccg ccaccagaag gcggatcgag ttcgtcccca tgtccaccgc
     4621 ggccacgcgc ccccgcggct cctcgtggat cggctccacg cgctcggcga cccaggcccc
     4681 gatcggattc gctccccccg ccaggtggtg ggcgtagtgc gcgtggaggc acttgatccc
     4741 cccgccgctc cccccgacgc cgctcaacgc ccccgcgccc ggaaacagcc gctcccgctc
     4801 cgccgcgtag tcgagcgccg cgtcctcgaa ccgctcccgg cactcggggt cctcctcgac
     4861 ccgctcgtcg agccgggcga tccatccctc gctctccaga cgcgacaccc ccgccaccgc
     4921 ctcgggacac gtgagccagt acagcgtggg gaacgggttc ccgtcccgat cgaccgggtg
     4981 gttgcggatc acgagcggat gccggccgcc gggacaccgc gcgacgacct cgaactgcgt
     5041 cgtcggctcg cgccccagct gctccgcgac ggcctggagg tccgagcttc gggtcagggc
     5101 ctcagcgccg cttgtcgcgg gtggacttgc ggacgtccac ctgacgctcc tcgctctgcc
     5161 gcatgaagcg cttcagcttc tgctcgaggc ctgggtcctg cccccggcgc agccgcttcg
     5221 gcggagggtc ctggagcgcc ttgatcgaga gatcgatctt gccgtcgtcc ttgatggcga
     5281 cgaccttggc ctcgacctcg tcggtctcgc ggagatggtc ccggacgtcg cggacgtagt
     5341 tgcggtcgat ctcggagatg tgcacgaggc cgaccttgcc gccttcgagc tcgatgaagg
     5401 cgccgtacgg ggtgatgcgc tggacggtgc cggtaacgac ggcaccgatc tcgaggtctg
     5461 cgatcggtcc tgctcccttc cggaaggggg gctgaatgcc cccgtcggcc tcgaactatc
     5521 ccacgcctct tccgagcccg tcaacgcgcc gccgggcttg gagggcggcg cgcccgcgcc
     5581 ttccgaaacc cggacagcct cggcggggtc ggtggcggtg ggcttcggct cggccggggt
     5641 cggggtcgat cggggtcggg gtcggggtcg gggtcggggt cgggtggggg ggcccctctc
     5701 ctcccaaaca ctcgcttgac ccgctcctcg caaagtgctt tagtgggtat aagtcagggg
     5761 ataccggaag gtagccatgc gcaaagcaag gcgaagccat cgagggcccg gtgattcggt
     5821 tcgggctgag cctgaagttc ggcccttcga cgagtccgca gcggcatggt tgatcggtga
     5881 aggcgttcgc tatctgaaca gcgcagacaa ggaaggcgag ctcgcgtatc ggcgggtgac
     5941 agagttgctc aaggaccgca atgacacggt ggagattctg accaccatac ttcgacaagc
     6001 cggtgggggc gacgcaccgt tgcgctggaa cctcttctat ctgcttggcg acgttgggga
     6061 tgcgggggcg gcagaccttc tagcggaggc ctcgctcgag gctttgccag acgagagctc
     6121 ggaggatgcc tgcgagggac cccgtgatca ggaattgctg gtgcgcacca tggcggttca
     6181 tgccctgggt cgcgtagcag gtcgccatcc agaagccgga gagcaacttc tcaggatcat
     6241 tggtga
//
LOCUS       DASUTW010000278         5293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_611180, whole genome shotgun sequence.
ACCESSION   DASUTW010000278 DASUTW010000000
VERSION     DASUTW010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611180"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..157
                     /locus_tag="VF058_09355"
     CDS             <1..157
                     /locus_tag="VF058_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6208545.1"
                     /translation="WVTEQAIQVLGGYGYIKDFPVEKWYRDAKIYTLFEGTSEIQRLV
                     ISRALAG"
     gene            complement(159..386)
                     /locus_tag="VF058_09360"
     CDS             complement(159..386)
                     /locus_tag="VF058_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208546.1"
                     /translation="MARRPAEPTPRRRRGRRRQASLWHLFLGFLGVLATIAFMSVVSQ
                     PWRLAVLALGLAILGYMAAVYGYDSRDGKDW"
     gene            complement(415..978)
                     /locus_tag="VF058_09365"
     CDS             complement(415..978)
                     /locus_tag="VF058_09365"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX6208547.1"
                     /translation="MKTPSHTGRRARGLLALGLVAALVLPFAGSAHSHPIDKFWRYGK
                     DVRHHKWREHPIGQRKHKRWHRNHPDATRREHRRFHHRRLVHPNRSKHRFRIVAKQSG
                     QASYFSGRVGACGKTLTGLYAAHRSWPCGSRVSVKRGHRYVIVRVLDRGPYTRGRVID
                     LSPAAFDRLGSLREGVMDVRIFRLKKR"
     gene            1162..2115
                     /locus_tag="VF058_09370"
     CDS             1162..2115
                     /locus_tag="VF058_09370"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020572463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX6208548.1"
                     /translation="MLEEVTLAAPRGYCAGVERAVDTVERALQLYGAPIYVRKQIVHN
                     LHVVRDLESKGAIFVEEETEVPEGATVVLSAHGVAPEVYRNAAARGLHVIDATCPLVT
                     KVHLEARRFAKEGYTILLIGHEGHEEVVGTSGEAPDHIRLVGSKEEARTVEVGDPSKV
                     AYLSQTTLSVDETNEIIEVLRERFPSIVGPPRDDICYATQNRQDAVKALAEDVGLVLV
                     IGSQNSSNSKRLAEVATDYGAPAHLIDDESEIDAAWLEGVERVGVTSGASAPEWLVER
                     VVERLRELGAPEVGQLQVAEEHVRFGMPYGVRDKDLRAAGV"
     gene            complement(2116..3312)
                     /locus_tag="VF058_09375"
     CDS             complement(2116..3312)
                     /locus_tag="VF058_09375"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEX6208549.1"
                     /translation="MDDSPRRNVKDLLAEAKDTSELMVDLAYAAVFFNDEDLADEVDA
                     LEELMQGYIHDLNWISMLAARSPEDAEAMASVLAIARAIESIGDAAADIARVVTVKLG
                     IPTMLRADLRHADEIVGRVKVREESEVVGRSLRDLTLPTETGMWVIAIRRGMEWETDP
                     GPDSVVSAGDVLLYQGPEEGTNLIRDQAGAPPLPSPPDEEPLALSELDRAVDLLVEMK
                     NSAEVAVGLAYSSLLFREPALAAEVAALETRSDTLLDDLESWVLRASPEARNPDELRG
                     LLRLGYASEMVFDAAREMTRAVESGDELHPVVQAALEATDEVAVETIVEAGSGAEGAT
                     LKDLSLETEAGMLALAIQRGRRWIHRPRPRFRLEAGDRVIAIGPEDGIPGLEGVTSAP
                     AESTTG"
     gene            complement(3341..4555)
                     /locus_tag="VF058_09380"
     CDS             complement(3341..4555)
                     /locus_tag="VF058_09380"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX6208550.1"
                     /translation="MPRVRLLSYWRAEQRTIRQGLVAMLLASVAMLGAGVALGFITDT
                     LESLPGLMVMVPAAIAARGNVFGALASRLGTAIHAGLFTTTREREGVLYQNVFAAAAL
                     TLSISLLMAVLAKTLSATFGVTSISILDFALISILGGVLASVVVLGLTIVLAVTAQRR
                     GWDLDSVAAPLITAAGDILTVPAIFLATFAVGYRWVSPTLTAILTVVTLVAAIRAAIT
                     DLPATRRIARESLPVLTIAATIDILAGLMIESRIEQFVAFPVLLILIPPILGDAGGLG
                     GILSSRLASKLHLGVITPRGLPEAAALSDLSIVALFAVVVFPLVGVAAHVVAGLVGIA
                     TPGMGTVVGVSLLAGLLATAAASVVAYYAAIASFRMGMDPDSHGIPLITSSMDLAGVV
                     ALILAILAFGIG"
     gene            4736..5053
                     /locus_tag="VF058_09385"
     CDS             4736..5053
                     /locus_tag="VF058_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208551.1"
                     /translation="MSGAYIQEVRPFTTVNVALPPADQSGGVKYGATWLSLGTDFGTA
                     TVRLAIGRPGDFRIEDNLQVPTGRQVVRRLRGGIVDQVASIVHLGGPPVSVLVEFETR
                     PDF"
     gene            5104..>5293
                     /locus_tag="VF058_09390"
     CDS             5104..>5293
                     /locus_tag="VF058_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6208552.1"
                     /translation="MTAQEGVIEVAGLRMRYGSVDVLDSVTFSVSRREVVVLLGPNGA
                     GKTTTIEVVEGFRVRSAGD"
BASE COUNT          830 a         1769 c         1944 g          750 t
ORIGIN      
        1 ctgggtcacc gagcaggcga tccaggtcct cggcggctac ggctacatca aggacttccc
       61 cgtcgagaag tggtaccggg acgccaagat ctacaccctg ttcgagggga ccagcgagat
      121 ccagcgcctc gtcatcagcc gagcgctggc cggctgagct accagtcctt cccgtccctg
      181 gagtcgtagc cgtagacggc tgccatgtag ccgaggatgg cgagcccgag ggccagcacg
      241 gcgaggcgcc agggctggct cacgaccgac atgaaggcga tcgtggccag caccccgagg
      301 aaccccagga agaggtgcca caggctcgcc tgcctgcggc gacctcgtct cctcctcggg
      361 gtcggttccg ccgggcgcct cgccatgcga cctcctcgag gtcatcctaa cgggctaccg
      421 cttcttcagc cggaagatcc tcacgtccat gaccccttcg cgcaggctcc ccagccggtc
      481 gaaggcggcg ggggagaggt cgatcacccg accccgcgtg tagggccctc gatcgaggac
      541 ccggacgatc acgtaccggt gtccgcgctt caccgagacc ctcgacccac agggccacga
      601 ccggtgcgcg gcgtacaggc cggtgagcgt cttgccacac gctcccaccc ttccactgaa
      661 gtaggaggcc tgtccggact gtttcgctac gatccggaac cggtgcttac tccggttcgg
      721 gtggacgagg cggcggtgat ggaagcggcg atgctcccgg cgggtggcgt cggggtggtt
      781 gcggtgccag cgcttgtgct tgcgctggcc gatcgggtgt tcccgccact tatggtggcg
      841 gacgtccttc ccgtagcgcc agaacttgtc gatggggtgg ctgtgggccg acccggcgaa
      901 gggaaggacc agtgcggcga cgagtcccag ggccagcagc ccccgggccc gtcgcccggt
      961 atgcgagggc gtcttcatgc gtacctcctg ttcggctcgc atcgcccctc caccccgacc
     1021 tcgcccgcac gcagacggac gaacagcccg agagggagga atcgagcgca cgatcgtgcg
     1081 cgccagcagg gtacagcaag ttcggggaaa cgccacaggc ccgtcggcgt gggtgcggga
     1141 ggcatcgggt agcatggggt cgtgctcgag gaggtcaccc tcgcggcccc acggggctac
     1201 tgcgccggcg tggagcgagc cgtggacacg gtggagcggg ccctccagct ctacggcgcc
     1261 cccatctacg tgcggaagca gatcgtgcac aacctccacg tggttcggga cctcgagtcg
     1321 aagggcgcga tcttcgtcga ggaggagacc gaggtccccg agggggccac cgtggtcctc
     1381 tcggcccacg gggtggcgcc cgaggtctac cgcaacgccg ccgcgcgcgg cctccacgtg
     1441 atcgacgcca cgtgcccgct cgtgaccaag gtgcacctcg aggcgcgacg tttcgccaag
     1501 gagggctaca cgatcctgct gatcggacac gaggggcacg aggaggtcgt ggggacgagc
     1561 ggcgaggccc ccgaccacat ccgtctcgtc ggatccaagg aggaggcccg gacggtcgag
     1621 gtcggcgacc cctcgaaggt ggcctacctc tcccagacca cgctctcggt ggacgagacg
     1681 aacgagatca tcgaggtcct gcgcgagcgc ttcccctcga tcgtggggcc gccccgcgac
     1741 gacatctgct acgcgaccca gaaccgccag gacgcggtga aggcgctggc cgaggacgtg
     1801 ggcctcgtcc tcgtgatcgg gtcgcagaac tcctcgaact ccaagcggct ggccgaggtg
     1861 gcgaccgatt acggcgctcc cgcccacctg atcgacgacg agtccgagat cgacgcggcc
     1921 tggctcgaag gggtggagcg ggtgggggtc acgtcgggcg cgtccgcccc cgagtggctg
     1981 gtggagcggg tggtggagcg gctccgcgag ctgggcgccc cggaggtggg ccagctccag
     2041 gttgccgagg agcacgtccg tttcgggatg ccctacggcg tccgggacaa ggacctgcgg
     2101 gccgccggcg tctgatcagc cggtcgtgga ctcggcaggc gcggaggtga ccccctccag
     2161 gccggggatc ccgtcctcgg ggccgatcgc gatgacgcgg tcgccggcct ccagccggaa
     2221 ccgaggcctc ggccggtgga tccaccgacg cccccgctgg atcgccagcg ccagcatccc
     2281 cgcctcggtc tccagcgaga ggtccttcag cgtggcgccc tcggcgcccg agccggcctc
     2341 cacgatggtc tccaccgcga cctcgtcggt cgcctccagc gcggcctgga ccaccgggtg
     2401 cagctcgtcg ccgctctcca cggcccgggt catctcgcgg gcggcgtcga agaccatctc
     2461 cgaggcgtag cccaggcgca gcagcccgcg gagctcgtcg gggttgcggg cctcgggtga
     2521 ggctcgcagg acccaggact cgagatcgtc gagcagcgtg tcggagcggg tctcgagggc
     2581 cgccacctcc gccgccagcg cgggctcccg gaagagcagg gacgagtagg cgaggccgac
     2641 ggcgacctcg gccgagttct tcatctccac gaggaggtcc acggcccgat ccagctccga
     2701 gagtgcgagc ggctcctcgt cgggcggcga ggggaggggc ggcgcgcccg cctggtcgcg
     2761 gatcaggttc gtgccctcct cggggccctg gtacaggagc acgtcgccgg ccgagaccac
     2821 ggaatcgggc cccgggtcgg tctcccactc catgccccgg cggatcgcga tcacccacat
     2881 cccggtctcg gtggggagcg tgagatcgcg gaggcttcgg cccacgacct cggactcctc
     2941 tcggaccttg acccggccca cgatctcgtc ggcgtggcgc aggtcggcgc ggagcatcgt
     3001 cgggatcccg agcttcacgg tgacgacgcg cgcgatgtcg gcggcggcgt ccccgatgct
     3061 ctcgatcgcc cgggcgatgg ccaggacgct ggccatcgcc tcggcgtcct cggggctgcg
     3121 cgcggccagc atcgagatcc agttgaggtc gtggatgtat ccctgcatca gctcctcgag
     3181 ggcgtcgacc tcgtcggcca ggtcctcgtc gttgaagaag accgccgcgt aggccaaatc
     3241 caccatgagc tccgaggtgt ccttcgcctc ggcgaggaga tccttgacgt tgcgtcttgg
     3301 gctgtcgtcc atctgccgcc tcccgagtat acggccgctc ctatccgatc ccgaaggcga
     3361 ggatggcgag gatgagcgcc accacgccgg caaggtccat cgagctggtg atcagcggga
     3421 tgccgtggct gtccgggtcc atccccatcc ggaagctggc gatcgccgcg tagtaggcga
     3481 cgaccgaggc ggcggcggtg gcgaggagcc cggcgaggag gctgacgccg acgacggtcc
     3541 ccatcccggg ggtggcgatc ccgaccaggc ctgccacgac gtgagcggcc acgcccacca
     3601 gcgggaagac gaccaccgcg aacagggcga cgatcgagag gtcggagagc gcggcggcct
     3661 ccgggaggcc tcgaggtgtg atcacgccca ggtggagctt cgaggccagg cgtgaggaga
     3721 ggatgccgcc cagtccgccc gcgtccccca ggatcggcgg gatcaggatc aggaggacgg
     3781 ggaaggccac gaactgctcg atgcgcgact cgatcatgag gcccgcgagg atgtcgatcg
     3841 tagcggcgat cgtgagcacg gggaggcttt cgcgggcgat gcggcgggtg gcgggcaggt
     3901 ccgtgatcgc cgcgcggatc gccgcgacga gcgtgaccac cgtcaggatc gccgtgagcg
     3961 tcggggacac ccagcggtag cccacggcga atgtggcgag gaagatcgcg ggcaccgtga
     4021 ggatgtcgcc ggccgcggtg atgagcgggg cagccacgct gtcgaggtcc cagccccggc
     4081 gctgtgcggt cacggcgagc acgatcgtca ggccgagcac cacgacgctg gccagcacgc
     4141 cccccaggat cgagatgagg gcgaagtcga ggatcgagat gctcgtgacg ccgaaggtgg
     4201 cggagagggt cttggccagg acggccatca ggagtgagat cgagagggtg agcgcggcgg
     4261 ctgcgaagac gttctgatag aggacgccct ctcgttcgcg cgtcgtggtg aagaggccgg
     4321 cgtggatcgc ggtgccgagc cgggaggcca gcgcgccgaa gacgttgccc cgagcggcga
     4381 tcgccgccgg gaccatcacc atcaggccgg ggagcgactc cagcgtgtcg gtgatgaagc
     4441 cgagcgcgac gccggccccc agcatggcga cggaggccag gagcatcgcc accagcccct
     4501 ggcggatcgt ccgctgctcc gcacgccagt acgagagaag gcgaaccctc ggcatctgtc
     4561 acccgtcatc gctcgacgtc tcctggggga gtgccccggg aagtatcgcc cgtcgcgggg
     4621 agccgctcaa cagcgggcgc gcgcgtggga ccggggctgt agggagaacg accggttggc
     4681 tccccctcga tggaggacta caagtgacgt caaacctcga cggggaggag gagggatgtc
     4741 aggagcctac atccaggagg ttcgaccgtt caccaccgtg aacgtcgcgc tgcctccggc
     4801 cgatcagtcg ggaggggtga agtacggcgc gacgtggctc tcgctcggca cggacttcgg
     4861 gaccgcgacc gttcggctgg cgatcgggcg gcccggcgac ttcaggatcg aggacaacct
     4921 gcaggtgccc accggtcgcc aggtggtgcg gaggctgagg ggtggcatcg tggatcaggt
     4981 cgcctcgatc gtgcacctgg gaggtccgcc ggtttcggtg ctcgtggagt tcgagacccg
     5041 gccggacttc tgatcggatc gctgccggcc ggagcgcttc ggtgagtatg ctccggagca
     5101 ggcatgaccg cacaggaagg agtgatcgag gtcgccggcc tccgcatgcg gtacgggtcg
     5161 gtcgacgtcc tggacagcgt gaccttctcc gtcagccgaa gagaggtcgt ggtcctgctg
     5221 ggaccgaacg gcgcggggaa gaccacgacc atcgaggtcg tcgaggggtt ccgcgtccga
     5281 tcggccgggg acg
//
LOCUS       DASUTW010000279         3661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_92917, whole genome shotgun sequence.
ACCESSION   DASUTW010000279 DASUTW010000000
VERSION     DASUTW010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92917"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..782
                     /locus_tag="VF058_09395"
     CDS             258..782
                     /locus_tag="VF058_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208553.1"
                     /translation="MSDELEPQGIKRREFIRKAAVTGATVAWAAPVVRTLAGSPAFAQ
                     TATPQGKDFSYIALCITCNGSHCRIKIDLDDNGNKAQCETGDNFQTPGCSLSCGASSN
                     CPSGACADIQATSPDGGKTVTVTLPAGCSIPHDGVAVGKCGNPPENGECVTTFVTGTD
                     GSGHTTVTFSMCDV"
     gene            complement(841..1614)
                     /locus_tag="VF058_09400"
     CDS             complement(841..1614)
                     /locus_tag="VF058_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208554.1"
                     /translation="MRTLEVGSLWIGVRVSSDAAERAVAGAFEPFLVEGPDPEPNFSV
                     RFEDASDGIRPFQILYRGDCQVVRTTHRSRVIRALRSSIARFAGEGSSLFRLRQVVLA
                     SQAGAILIPPDLLPRVEAIEADLRERGIEVLDPAFALLDPATGEVVIPERAIPLGEDA
                     LGALDADGDGPPLPMDGEGARFPVAHWQVIRRDREEPLTRMDVFAALMPSIEEEADVP
                     IADAMRAVAGALSTASVGAVYHQKGEELLGTLAGLPGAG"
     gene            complement(1648..2028)
                     /locus_tag="VF058_09405"
     CDS             complement(1648..2028)
                     /locus_tag="VF058_09405"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX6208555.1"
                     /translation="MTEKLQPKDVEPEAAPRPRATVAHVEIDGEGVLYDEVSNQIHLL
                     NPIATLIWSGLDGRTTLEELSAELSDAFGADLARVRFDVLEAVRGFAEQDLLEDEGAP
                     PIEEPEAAQEASDEPRYLKDPPSP"
     gene            complement(2025..2984)
                     /locus_tag="VF058_09410"
     CDS             complement(2025..2984)
                     /locus_tag="VF058_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208556.1"
                     /translation="MSDRDTDPASATALEEQRRALEEQRRALDDAPWSYRGPRLHALG
                     FDFGIRANDEEVGAYLGSLFESFEADGEPEHVYSFIDRPGEEMRYEIYFDGEHVVSSR
                     EAGGALRYFQWDVNERVIARNRGYILIHAASAADEAGRGIVMPAAMDSGKTTLVSGLV
                     RAGLRYLSDEAAAVDPRTGVLHAYAKALTIDPGSWDVLSELRPSLEGPLAPFAQDQWY
                     VPVGSIRDGAVASSARPAFVIAPRYVGEGRSELLPMSRAEALQALVENAFNFRLFDGR
                     MALRALADVVRGAECYRLMVAELTEACDLVLGLVEASGEGRTE"
     gene            3036..>3661
                     /locus_tag="VF058_09415"
     CDS             3036..>3661
                     /locus_tag="VF058_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6208557.1"
                     /translation="MVGVFLVDPEPFFGEGLSAAIDGEGGMEVLGRTTDEREALRMCA
                     QRSPDVVLTEVALDAGSGLNLARRLGGDTRVVVLTRRPEGDVLLDSVDAGAAGCLGHD
                     LSVERLLVHLRDLVDVGRFAVNPDTLHETLLRATATATGDAHGVGRLTPREREVLSLV
                     AQGLDNARIAKALYLSPHTVRTHVGNILRKLEVHSRAEAARVAIHAWDR"
BASE COUNT          566 a         1336 c         1234 g          525 t
ORIGIN      
        1 cgcgtcgctg gtcggagagc tcgacgcgct ggggctggtt tccgggcagc cggaggccga
       61 ccgcccgacc gagtgactag acccccctac gccctgcatc gtagagaccc gatccgtcac
      121 ccctctgacc tgtggcgctg gtgcggcggg cttccccgac gtcgcgaccg gacttacgcc
      181 cgaccgcgta tcgcgttcgc ggcaccaccg cctctaccat ccagaccacg aggaacgaga
      241 acgagaggag tcagggaatg agtgacgagc tcgaacccca gggcatcaag cggcgggagt
      301 tcatccgcaa ggccgcggtg acgggtgcga cggtcgcgtg ggcggctccg gtcgtgcgga
      361 cgctggcggg ctcgccggcc ttcgcccaga cggcgacccc gcaggggaag gacttctcct
      421 acatcgcgct ctgtatcacc tgcaacgggt cgcactgccg gatcaagatc gacctggacg
      481 acaacggcaa caaggctcag tgcgagactg gggacaactt ccagaccccg ggctgcagcc
      541 tcagctgcgg cgcctcgtcc aactgcccgt cgggcgcatg cgcggacatc caggcgacct
      601 cgccggacgg cggtaagacg gtcaccgtga cgcttcccgc gggctgctcg atcccgcacg
      661 acggcgtcgc cgtcggcaag tgcgggaacc cgcccgagaa cggcgagtgc gtcaccacct
      721 tcgtgaccgg gaccgacggc tccggccaca cgacggttac gttcagcatg tgtgacgtgt
      781 aagacccggt ggatcaaccg agggcccggc ccggcgtcgg gccctcggtc gtgtccgggg
      841 tcagccggcc cctggaaggc cggcgagggt gcccaggagc tcctcgccct tctggtgata
      901 gaccgccccg accgacgccg tggagagcgc ccccgccacg gcccgcatcg cgtccgcgat
      961 cgggacgtcg gcctcctcct cgatcgaggg catcaaagcg gcgaagacat ccatccgagt
     1021 gaggggttcc tcgcggtccc ggcggatgac ctgccagtga gcgaccggga agcgggcacc
     1081 ctccccgtcc atcgggagcg gcggaccgtc tccgtccgcg tcgagggccc ccagcgcatc
     1141 ctcgcccagg gggatggccc gctccgggat cacgacctcg ccggttgcgg ggtccaggag
     1201 ggcgaacgcc gggtccaaga cctcgatccc tcgctcgcgg aggtccgcct cgatcgcctc
     1261 cacccgcggc aggagatcgg gcgggatcag gatcgctccg gcctgcgagg cgaggaccac
     1321 ctggcgcagc ctgaagagac tcgatccttc cccggcgaac ctggcgatcg aggaccggag
     1381 cgcccggatg acccgggacc gatgggtcgt ccggacgacc tgacagtcac cccgatacag
     1441 gatctggaag ggccggatgc cgtcagacgc gtcctcgaac cgaacggaga agttcggctc
     1501 gggatcgggg ccctcgacca ggaagggctc gaaggccccc gccaccgccc gctcggcggc
     1561 gtccgagctg acgcggacgc cgatccagag cgatccgacc tcaagggtcc tcattgccgt
     1621 ccagaccaga cggtcgatgc tgagcattca tgggctgggg ggatccttca ggtaacgtgg
     1681 ctcgtcgctg gcctcctggg ccgcctccgg ttcctcgatt gggggggcgc cttcgtcctc
     1741 gagcagatcc tgctcggcga accctcggac cgcctcgagc acgtcgaagc ggacgcgggc
     1801 aaggtcggcg ccgaaggcgt cgctcagctc ggccgagagc tcctcgagcg tggtccttcc
     1861 gtccagcccg ctccagatca gggtcgcgat ggggttcagc aggtggatct ggttgctcac
     1921 ctcgtcgtag agcacgccct cgccgtcgat ctcgacgtgg gccaccgtcg ccctcggccg
     1981 cggggcggcc tcgggctcga cgtccttcgg ctgcagcttc tcggtcactc cgtcctcccc
     2041 tcgcccgacg cctcgacgag cccgagcacg agatcgcagg cctccgtcag ctccgccacc
     2101 atgagccggt agcactcggc tccacgcacg acatcagcga gggccctcag cgccatccgc
     2161 ccatcgaaca ggcggaagtt gaacgcgttc tccacgagcg cctgcagtgc ctcggccctc
     2221 gacatcggca gaagctcgct cctgccctcg cccacatacc gaggcgcgat cacgaaggcg
     2281 ggccgagcgg atgacgccac cgccccgtcc cggatcgatc ccaccggcac gtaccactgg
     2341 tcctgcgcga acggcgcgag cggcccctcc aacgagggac ggagctcgga gagcacgtcc
     2401 caggaccccg gatcgatcgt cagcgccttg gcgtaggcgt ggagcacgcc cgtccgcgga
     2461 tccacggccg ccgcctcgtc gctcaggtat cggaggccgg ccctcaccag cccagagacg
     2521 agcgtggtct tccccgagtc catcgcggcc ggcatcacga tgccccgccc cgcctcgtcc
     2581 gccgcggacg cggcgtggat caggatgtag ccacggttcc gggcgatcac gcgctcgttc
     2641 acgtcccact ggaagtagcg cagcgccccg ccggcctccc gggagctgac gacgtgctcg
     2701 ccgtcgaagt agatctcgta acgcatctcc tcccccggtc gatcgatgaa ggagtacacg
     2761 tgctccggct ccccgtccgc ctcgaaggat tcgaagaggc ttcccaggta ggcgccgacc
     2821 tcctcgtcgt tcgcccggat cccgaagtcg aatccgagcg cgtgaaggcg cggaccgcga
     2881 taggaccacg gggcgtcgtc gagcgcacgc cgctgctcct cgagcgcacg ccgctgctcc
     2941 tcgagcgccg tggcgctcgc cgggtcggtg tctcgatcgc tcatctcggt cctcgtcggg
     3001 gagaggccca acccgtgcac tgtacgatgt cgaccatggt aggggtcttc ctcgtcgatc
     3061 ccgagccctt cttcggagag gggttgagcg ccgcgatcga cggtgagggt gggatggagg
     3121 tcctcggcag aaccaccgac gagcgggagg ccctgcggat gtgcgcccag cgctcccccg
     3181 acgtggtgct caccgaggtg gccctcgacg ccggatccgg cctcaacctg gcccggcggc
     3241 tcggcggcga cacgcgggtc gtcgtgctta ctcgacgacc cgagggcgac gtcctcctcg
     3301 actcggtgga tgcgggcgcg gccgggtgcc tcggacacga tctctcggtt gagcggctgc
     3361 tcgtgcatct gcgggacctg gtcgacgtcg gacggttcgc ggtgaacccc gacaccctcc
     3421 acgagacgct gctgcgcgcc accgcgacgg cgacggggga cgcgcacggg gtcggccggc
     3481 ttaccccccg ggagcgggag gtcctgagcc tggtggccca aggcctcgac aacgcgcgga
     3541 tcgccaaggc cctctatctc agcccccaca cggtccgaac ccacgtgggc aacatcctga
     3601 ggaagctcga ggtgcactca cgagccgaag cggcccgcgt cgcgatccac gcgtgggatc
     3661 g
//
LOCUS       DASUTW010000280         6075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_94415, whole genome shotgun sequence.
ACCESSION   DASUTW010000280 DASUTW010000000
VERSION     DASUTW010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94415"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VF058_09420"
     CDS             <1..391
                     /locus_tag="VF058_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX6208558.1"
                     /translation="GVGRRLKEHDPSVQVIAAEPELGDSVYGLRSLEEGFVPPILDES
                     LLDGKHLVAAGDALLATRMLAEQEGIFAGISSGAVLHVARKVGARAAEGDIVCLLPDG
                     GWKYLSTGAWTGAASEAEKRVEETVWW"
     gene            385..1206
                     /gene="murI"
                     /locus_tag="VF058_09425"
     CDS             385..1206
                     /gene="murI"
                     /locus_tag="VF058_09425"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX6208559.1"
                     /translation="MVGDARAIGMFDSGVGGLTVARSVLDLLPREPLVYYGDTARAPY
                     GPRPLDEIRAFSREIAAYLLHRDVKILVVACNSVEVAAIEDVTAEAGVPVVGVIDPGV
                     RAAARVTRSGRIGVIGTEATIASGAYQRAADRHGIDARLVARACPSFVPFVERGDTTS
                     EELLQAARGYLEPVRDAGVDTLILGCTHYPLLAGMIRMVMGPDVVLISSAEETARDVY
                     AALVRDDRLREEPGPPRHEFLCSGDPDRFRELGRVFLGPEMDDVALASVQREPLG"
     gene            1206..1964
                     /locus_tag="VF058_09430"
     CDS             1206..1964
                     /locus_tag="VF058_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6208560.1"
                     /translation="MELTVLGSDGTWPRADGAASGYVVSHDGYVVWQDMGTGTMAHLQ
                     RHLGLLDVDAVIVSHVHADHFVDLFPYFYARRYGSETPAPRIPLVVPTGTRERIRSML
                     SDAGWEEFPDCFEINEIEPGEGLDLGPFRLSTARMAHPVPTLGVRYEADGVALAYSAD
                     TGPTDELVELARGADLLVAEATWLEDGKVYPPDLHMTAKEAGEQAAKADAGRLMLAHI
                     QPIFDPDHSREKASEVFDGPVGVARPDVRWEAGA"
     gene            1961..2680
                     /gene="rph"
                     /locus_tag="VF058_09435"
     CDS             1961..2680
                     /gene="rph"
                     /locus_tag="VF058_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX6208561.1"
                     /translation="MSRPDGRGPRELRPIRYETEYLAFAEGSVLLEMGGTRVLCAATW
                     EPRVPRWLDGTGKGWVTAEYSMLPRSTKERTPREVNRGRPSGRTQEIQRLIGRSLRAV
                     TDLERMGERSITVDCDVLQADAGTRTASITGGYVALALALRSLEEQGLAPSGVLTDSV
                     AAVSTGIVGGLPLLDLCYDEDAEAEVDFNVVMTGSGKLVEVQGTAEGDPFSRDQLEEM
                     LTLAEEGIARLTEIQREALGS"
     gene            2677..3267
                     /gene="rdgB"
                     /locus_tag="VF058_09440"
     CDS             2677..3267
                     /gene="rdgB"
                     /locus_tag="VF058_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX6208562.1"
                     /translation="MSRAMPDRLVIASRNPGKIREIQAIASDWRVEWVTDADWPEVDE
                     TGRTYLQNAKRKAHAVAEATGLPALADDSGIEVDALGGAPGPRSARFAGEAADDERNL
                     RELLRAVAGVPGPGRTARYRAVAVIAWPDGEEAWAEGVCEGLLRDEPSGSGGFGYDPI
                     FEPVGWDRTMAELTSVEKDRISHRGRAIRALREELG"
     gene            3360..3512
                     /locus_tag="VF058_09445"
     CDS             3360..3512
                     /locus_tag="VF058_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208563.1"
                     /translation="MCLTCGCGEPNADHGDGRHITLNDLQAAAEAAEISADEAANNLQ
                     ETYKNA"
     gene            3572..4081
                     /locus_tag="VF058_09450"
     CDS             3572..4081
                     /locus_tag="VF058_09450"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEX6208564.1"
                     /translation="MALIQMRAPEGRPWKLREWIQGKPLGVPTHSMVVHFPVAFYLGV
                     IVFDVMSRITPNEGLVLASTYMLLLGLLGTLIAAPLGLIDWLGMVKGSSKRRLATRHM
                     VFQLLAAAFFIVALVLRWPDRTASQAEISWIVIEAVGYLVLIVGQHLGGLLVYQKAMR
                     VGTGGADRG"
     gene            4124..5044
                     /locus_tag="VF058_09455"
     CDS             4124..5044
                     /locus_tag="VF058_09455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6208565.1"
                     /translation="MARQDLHPGDVDDLRLYLEAAAREPLLTKEEEVELAMSLEHGKE
                     AEERLASGRIRSEKTKATLAADIKEAERSRKRFILANLRLVVSIAKKYQGQGLPLLDL
                     IQDGNIGLMRAIELFDWRRGFKFSTYATWWIRQAITRAIADRGRQIRIPVHVHERIRK
                     IRKTRERLMQQTGRDPTPDELSRAAGVPVEMLEELEAAVRKEPRSLQEPVGEDAELGD
                     LVAQVELESPLDAVEDTLLREEIAQAVGTTLEPMEQEVVSLRYGLGNGRAMSLREVAE
                     LVGVSAETVRKVERQALGKLRASDLLEEIW"
     gene            5048..5788
                     /locus_tag="VF058_09460"
     CDS             5048..5788
                     /locus_tag="VF058_09460"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX6208566.1"
                     /translation="MPVVVVGASSSVGRALVPLLVERAPEVRAIVRSPSQAEGLRALG
                     AKVAVARAPDPDALAMLFHDAHTVCHLAGGMRMPSAEAYEEANLETVRRTVEGARTAG
                     AERILFLSTSGADPEDANAYLRSKGRAEELVRGSGLEHAIVRATRLVAPASPWFRDVA
                     ALAARRPAAVVGDGSASLAPVASADVAAILAAADDRAGSISTTLGVEGPDVVTADDLV
                     GIVAGRRRRPRHLALGARASRAVGEPVA"
BASE COUNT          853 a         2070 c         2330 g          822 t
ORIGIN      
        1 gggcgtgggc cgccgtctga aggagcacga cccgagcgtg caggtgatcg cggccgagcc
       61 ggagctgggg gactcggtct acggcctgcg ctcgctcgag gagggcttcg tccccccgat
      121 cctcgacgaa tcgctgctcg acgggaagca cctggtggcc gccggcgacg ccctcctggc
      181 gacccggatg ctcgcggagc aggaggggat cttcgccggg atctcctcgg gcgccgtgct
      241 gcacgtcgcc cggaaggttg gggcacgggc cgcggaaggc gatatcgtat gcctcctgcc
      301 cgacggcgga tggaagtact tgtcgaccgg cgcctggacc ggcgccgcgt ccgaggcgga
      361 gaagcgggtg gaggagacgg tctggtggta ggcgacgcgc gagcgatcgg gatgttcgat
      421 tcgggtgtgg gcgggctgac cgtggcccgc tcggtgctgg atctccttcc gcgggagccg
      481 ctcgtctact acggcgacac ggcccgcgct ccctacggac cccgtcccct ggacgagatc
      541 cgggccttct cgcgcgagat cgccgcgtac ctgctccatc gggacgtaaa gatcctcgtc
      601 gtggcgtgca actcggtcga ggtcgccgcc atcgaagatg tgaccgccga ggccggcgtc
      661 ccggtggtgg gggtgatcga cccgggcgtg cgcgccgcgg ccagggtcac ccggagcggg
      721 cggatcggcg tgatcgggac cgaggccacg atcgccagcg gggcctacca gcgggcggcc
      781 gaccgccacg ggatcgacgc caggctcgtg gcccgggcct gcccgtcgtt cgtcccgttc
      841 gtcgagcggg gggacaccac gagcgaggag ctgctgcagg ccgctcgcgg gtacctcgag
      901 ccggtccggg acgccggcgt cgacacgctc atcctcggct gcacccacta ccccctgctc
      961 gccgggatga tccggatggt gatgggcccc gacgtcgtgc tgatctcgtc cgccgaggag
     1021 accgcgcgtg acgtctacgc cgcgctggtc cgggacgacc gtctgcggga ggagcctggg
     1081 cccccgcggc acgagttcct ctgctccggg gatcccgatc gcttccgcga gctggggagg
     1141 gtcttcctgg gaccggagat ggacgacgtc gcgctggcca gcgtccagag agagcccctc
     1201 ggctgatgga gctcacggtc ctcggctccg acgggacctg gcctcgcgcg gacggcgcgg
     1261 cgagcggcta cgtggtgagc cacgacggct acgtggtctg gcaggacatg ggcacgggga
     1321 cgatggcgca cctgcagcgg cacctgggat tgctcgacgt cgacgcggtg atcgtctcgc
     1381 acgtccacgc cgaccacttc gtggacctct tcccgtactt ctacgctcgc cgctacggat
     1441 ccgagacgcc cgccccccgg atcccgctcg tcgtccccac ggggacccgg gagcggatcc
     1501 ggtcgatgct ctccgacgcg ggctgggagg agtttccgga ctgcttcgag atcaacgaga
     1561 tcgagccggg ggaggggctc gatctcgggc cgttccggct gagcaccgca cggatggccc
     1621 acccggttcc cacgctgggg gttcgctacg aggccgacgg cgtggcgttg gcgtattccg
     1681 ccgacaccgg tcccaccgac gagctcgtgg agctggccag gggggccgat ctgctggtcg
     1741 ccgaggccac ctggctcgag gacgggaagg tgtacccgcc cgatctccac atgaccgcga
     1801 aggaggccgg cgagcaggcc gccaaggccg acgccggccg gctgatgctg gcgcacatcc
     1861 agccgatctt cgaccccgac cactcccggg agaaggcctc cgaggtcttc gacgggcccg
     1921 tgggcgtggc gcgcccggac gtccgctggg aggccggggc gtgagcagac ccgacggccg
     1981 cgggccgcgg gagctccgcc cgatccgcta cgagaccgag tacctggcgt tcgccgaggg
     2041 atccgtgctc ctggagatgg gggggacccg agtcctgtgc gcggccacgt gggagccacg
     2101 ggtgccgcgc tggctcgacg ggaccgggaa gggatgggtg acggcggagt actcgatgct
     2161 gccgcgctcg accaaggagc ggacccctcg ggaggtgaac cgcgggcgcc cctcggggag
     2221 gacccaggag atccagcggc tgatcggtcg cagcctccgg gccgtgaccg acctggagcg
     2281 gatgggggag aggtcgatca cggtggactg cgacgtcctg caggccgacg cagggacccg
     2341 gacggcctcg atcaccggcg ggtacgtcgc cctggccctg gctctccgga gcctcgagga
     2401 gcaggggctg gctccctcgg gcgtcctcac cgactcggtg gccgccgtga gcacggggat
     2461 cgtcgggggg ctgccgctgc tcgatctctg ctacgacgaa gacgccgagg ccgaggtgga
     2521 cttcaacgtg gtgatgacgg gctcggggaa gctggtcgag gtgcagggga ccgccgaagg
     2581 cgatcccttc tcgcgcgacc agctcgagga gatgctcacg ctggccgagg agggcatcgc
     2641 ccggctcacc gagatccagc gggaggcgct cgggtcgtga gccgggcgat gcccgaccgg
     2701 ctcgtgatcg catcccgcaa cccgggaaag atccgcgaga tccaggcgat cgcgagcgat
     2761 tggcgggtcg agtgggtgac ggacgccgac tggcccgagg tcgacgagac cggccgcacg
     2821 tacctccaaa acgcgaagcg gaaggcccac gcggtcgccg aggccacggg gctccccgcc
     2881 ctcgccgacg acagcgggat cgaggtggac gccctgggcg gtgcgcccgg gccccggtcc
     2941 gcccgcttcg ccggcgaggc cgcggacgac gagcgcaacc tccgagagct gctgcgcgcc
     3001 gtcgcgggcg tcccggggcc cgggcgcacc gcccgctacc gggccgtggc cgtcatcgcc
     3061 tggcccgatg gcgaggaggc ctgggccgag ggcgtctgcg agggcctgct ccgcgacgag
     3121 ccctcgggct cgggagggtt cggctacgac ccgatcttcg agcccgtggg ctgggaccgg
     3181 acgatggccg agctcacgtc cgtggagaag gaccgcatca gccaccgggg gagggccatc
     3241 cgggcgctgc gcgaggagct ggggtgacgg gcttgcgttt cgtaacgaca agcgatatta
     3301 gttttaggga agtgggaccg gccggcccgg ccggccattc gaccggaagg aggagcgcca
     3361 tgtgcctcac ctgcggatgt ggtgagccga acgccgacca cggcgacggc cggcacatca
     3421 ccctgaacga cctgcaggcg gccgccgagg ccgcggagat ctcggccgac gaggccgcga
     3481 acaacctgca ggagacctac aagaacgcct agactcggcc tggcccgggg ccccggccgc
     3541 ccggccgggg cctccggagg gaggaggacc gatggcgctg atccagatgc gggctcccga
     3601 ggggcgtccc tggaagctga gggagtggat ccagggcaag ccccttggcg tgcccactca
     3661 ctcgatggtg gtgcacttcc ccgtcgcctt ctacctgggc gtgatcgtct tcgacgtgat
     3721 gagccgcatc accccgaacg aggggctggt gctggcctcg acctacatgc tgctgctggg
     3781 gctcctgggc acgctcatcg cggcgcccct gggcctcatc gactggctgg gcatggtgaa
     3841 gggatcgtcc aagcggcggc tggccacgcg ccacatggtg ttccagctct tggccgcggc
     3901 gttcttcatc gtcgcgctcg tcctgcggtg gcccgatcgg accgcctcgc aggccgagat
     3961 ctcctggatc gtgatcgagg ccgtcggcta cctcgtcctg atcgtgggcc agcacctggg
     4021 cgggctcctc gtctaccaga aggcgatgcg cgtcggcacg gggggcgccg accgagggtg
     4081 agacctgcgg cctcggccgg cgctaggatg gccccagagg cgcatggcga ggcaggatct
     4141 ccatcccggc gacgtcgacg acctccgcct ctacctcgag gcggcggccc gcgagcccct
     4201 cctcacgaag gaggaggagg tcgagctcgc catgtccctg gagcacggca aggaggccga
     4261 ggaacgcctc gcctccggcc ggatccgctc cgagaagacg aaggcgacgc tcgccgccga
     4321 catcaaggag gccgagcgtt cgaggaagcg attcatcctg gccaacctgc gcctcgtggt
     4381 ctcgatcgcc aagaagtacc agggccaggg cctcccgctg ctggacctga tccaggacgg
     4441 gaacatcggc ctgatgcgcg cgatcgagct cttcgactgg cgacgaggct tcaagttctc
     4501 cacctacgcg acctggtgga tccggcaggc gatcacgcgg gccatcgccg accgcgggcg
     4561 ccagatcagg atcccagtcc acgtccatga gcggatccgc aagatccgga agacccgtga
     4621 gcggctgatg cagcagacgg ggagggaccc cacgcccgac gagctgtcgc gggccgcggg
     4681 cgtcccggtc gagatgctgg aggagctgga ggcggcggtg cggaaggagc cgcggtcgct
     4741 gcaggagccg gtgggggagg acgccgagct gggcgacctc gtggctcagg tcgagctgga
     4801 gagccccctc gatgccgtgg aggacacgct gctccgcgag gagatcgcgc aggccgtggg
     4861 gaccaccctc gagccgatgg agcaggaggt cgtctccctc cgctacggcc tggggaacgg
     4921 ccgggcgatg agccttcgag aggtggccga gctggtagga gtctcggccg agacggtgcg
     4981 caaggtcgag cggcaggcgc tggggaagct ccgcgcctcc gatctcctcg aagagatctg
     5041 gtagaccgtg ccggtcgtcg tggtcggcgc ctcgagctcc gtgggccgcg ccctcgtccc
     5101 cctgctcgtg gagagggccc ccgaggtccg ggccatcgtg cgcagcccgt cccaggcgga
     5161 gggcctccgg gccctggggg cgaaggtcgc ggtggcccga gcgccggacc ccgacgcgct
     5221 ggcgatgctc ttccacgacg cccacacggt gtgccacctc gcggggggga tgcggatgcc
     5281 gagcgccgag gcctacgagg aggccaacct cgagaccgtg cggcgcacgg tcgagggcgc
     5341 ccgcacggcg ggggccgagc ggatcctctt cttgtcgacc tcgggggcgg accccgagga
     5401 tgccaacgcg tacctgcgct cgaaggggag ggcggaggag ctcgttcggg ggtcgggcct
     5461 ggagcacgcg atcgtgcggg ccacccggct cgtggcgccg gccagcccct ggttccgcga
     5521 cgtggccgcg ctggccgcgc ggagaccggc cgcggtcgtg ggggacggct cggcctcgct
     5581 ggccccggtg gcctcggccg acgtggccgc gatcctggcg gccgccgacg accgggcggg
     5641 gtcgatctcg acgaccctcg gggtcgaggg gcccgacgtc gtgacggcgg acgacctcgt
     5701 cgggatcgtg gcgggaagga ggcggcggcc gcgccacctg gccctggggg cgcgcgcgtc
     5761 gcgcgccgtc ggggagccgg tcgcctagga ctatctggac ctcttggcgc gcgacgcgcg
     5821 cgccgacgcc cccgacgccg cggcggagtt cgacgtcccc ctcacaccgc ttcgcgaggc
     5881 gataggatgg gccgtccgcg ggcgacgcgc cgcctcggcg ggcggtcgag gggggcgtga
     5941 gccgtgccga taacactcgg ggtgggctca gggaggcgtt ccgcgtgagc aagcgatcac
     6001 tccggctcga ggagggtgtc gccctcgctc tccgggccgt ccgacagccg cggaccggcg
     6061 cggccgccgt gctgt
//
LOCUS       DASUTW010000281        14443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1260825, whole genome shotgun sequence.
ACCESSION   DASUTW010000281 DASUTW010000000
VERSION     DASUTW010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14443
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260825"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VF058_09465"
     CDS             complement(<1..252)
                     /locus_tag="VF058_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208567.1"
                     /translation="MVVGVLEVVVATSFAALIFSGPLAEHLPAGIGLGLVAAAAILIT
                     VALVSSHRGTFGSVQDISAAVLSIVAASIVADLPPEDPRI"
     gene            complement(441..935)
                     /locus_tag="VF058_09470"
     CDS             complement(441..935)
                     /locus_tag="VF058_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019985724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6208568.1"
                     /translation="MRLRNLTTEDLWLYEAIHCDSRMMEHLGGPLPKAGLAEKLERDA
                     AATEADEVWVLVIVLEEETAAGTVSIWEHEVGGELLTEIGWMVLPDHQGKGVGSAGVR
                     MALDRARETGRWDVLHAFPPVSNLASNAMCRTLGFTLLGERTNRFRDRQLRVNHWRLD
                     LRSA"
     gene            complement(940..1263)
                     /locus_tag="VF058_09475"
     CDS             complement(940..1263)
                     /locus_tag="VF058_09475"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX6208569.1"
                     /translation="MSARGHVLPRPRLSRRAAGLAAISFVLLLGLVFPLRQYVIQRSE
                     IAGLERQVQRLEEERVTLERRVERLRDPEYLERIARQCLGMVRPGEIAFVAVPEDGQP
                     LPASC"
     gene            complement(1260..2537)
                     /gene="eno"
                     /locus_tag="VF058_09480"
     CDS             complement(1260..2537)
                     /gene="eno"
                     /locus_tag="VF058_09480"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX6208570.1"
                     /translation="MPPIERIRAREVLDSRGNPTVEAEVELADGSLGLAAVPSGASTG
                     EHEALELRDGGDRFKGKGVLKAVENIHERIEPALQDVEALDQREVDRMLLDLDGTIDK
                     SGLGANAILAVSLANAKAAAVSSGLPLYRYLGGPNAHLLPLPLLNVINGGAHADNAIE
                     LQEFMLAPRGAASFSEAIRWGSEVFHTLKEQLHGKGLSTAVGDEGGFAPDLPGSAAAC
                     EALVGAIEAAGLEPGSDVAIAIDAAASEVFRDDAYHVEGEARSAEQMAEYYDRLVTDF
                     PIVSIEDPLDENDWEGWAAVTSLLGNRIQLVGDDLLVTNPERLSRAVAEASCNAILVK
                     PNQIGSLTETLDVVDAAVAASFGVVISHRSGETEDSTIADLAVATNAGQIKAGAPSRG
                     ERTAKYNRLLRIEEELGGTARYAGAAVYRRFGR"
     gene            complement(2585..3262)
                     /locus_tag="VF058_09485"
     CDS             complement(2585..3262)
                     /locus_tag="VF058_09485"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6208571.1"
                     /translation="MDRAAWIVELRRVNEREEDALAPTYDQLWGEIDEPHRAFVERFL
                     SMVPPAGRVLDAACGTGKYLGMVLESGRSVLGVDHAGASLVEAKTKFPKAETERHDLQ
                     DLPYRDAFDGVMCVDAVEMIPPEDWPEVLRRFRRAIRGSGPLYLTVEVAPPEEVRAGT
                     EGGRRRGLPLVDGEVIWEEHGYHHYPSLDRVRTWLGEAGFAIEGEAEQLLEDEGFAYR
                     HVLARAV"
     gene            complement(3279..4175)
                     /locus_tag="VF058_09490"
     CDS             complement(3279..4175)
                     /locus_tag="VF058_09490"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6208572.1"
                     /translation="MTHLLSLVGIVSISFSAIFVRLADVSPDTAAFFRAAYAVPALVL
                     VWLVTRRRDERTPRERWMAFGAGLFLALDFSFWHRAIGDIGAGLATVIGNTQVVFVGI
                     YAWIFLRERPTRLAFVLVPTVFLGVLLISGLGREDAFGSNPLGGVFWGVLTGLGYTGF
                     LILLRRSNRSLSPPAGPMMEATTGALLGMAILAPIFDPAFSVVPTWPAHGWLLALALI
                     CQVFGWLLITVALPRLPSLETSVMLLLQPMLTVLWAYLLFSEILSIVQWLGVALALIG
                     VGTLAAKGSVEPDVERDTVLQQ"
     gene            complement(4172..5026)
                     /gene="mazG"
                     /locus_tag="VF058_09495"
     CDS             complement(4172..5026)
                     /gene="mazG"
                     /locus_tag="VF058_09495"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX6208573.1"
                     /translation="MAEVHGTPDPLDHVPTGPVPSGRGGERLLDLVRVVARLRAPDGC
                     PWDRAQTHMSLRRHLLDEAYELIEAIEEGTPDDLKEELGDVLLQAIFHAQMAADEGAF
                     DVDDVAEVTVEKLIRRHPHVFAEVDADTPEAVYANWERIKAEEKGERVVDEGVPKALP
                     ALIAAQKVQRRASGRGFDWAALAGAVEKVREELAELEEAAAAHAGRDVEGEAKDALEE
                     EVGDVLFAVTALARKLGVDAESALRSTIRKFVIRFETMAERAREEGVRLEDLAEDDLL
                     RRFREARA"
     gene            complement(5091..6035)
                     /locus_tag="VF058_09500"
     CDS             complement(5091..6035)
                     /locus_tag="VF058_09500"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX6208574.1"
                     /translation="MRLRLLSLLAAASLVLSACTAFEPAAAVVGPHRIEDEEFLRLLD
                     FIMADPQFAEAAVANPDVERATLQRQLLTFLIQHEVVEERAREEGIQVDRQRVEELMQ
                     DQAIQDQIGRSDATAQDVRAFVRAQLIRSDVGQAVVEDEASDERLQAAYEERAREFTE
                     VHASHILVGSEEEASRIVAEATPSNFARLARELSDDQGSGANGGDLGTRAAGAYVPEF
                     ADALLELPEGRIGGPVETQFGFHVILVHSREAIPFEQAREQLLQELGPEVFREWMLER
                     LRSLTVRVNPRYGTFDEDTGEVVERRATTPLPGPQTTP"
     gene            complement(6046..9366)
                     /gene="mfd"
                     /locus_tag="VF058_09505"
     CDS             complement(6046..9366)
                     /gene="mfd"
                     /locus_tag="VF058_09505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX6208575.1"
                     /translation="MRKLLEPLIESEALQAVLARPGPVRVDPAGHAYAIAALAEALEG
                     PVLAVTGGPRESAPLAHDVAAYLGADRAAHLPAWEALPVEGISPSPETSARRHAAIRR
                     AREASGPFVLVAPAVAVVQRLLPHVGSVDPLVVQQGEEIEPDELADRLVALGYTRADL
                     VTHRGEFAVRGGIVDVFPGAAPRPVRIELFGDEVESLREFSASTQTSTGRLPRMEADP
                     VRELLPTPEVMERALEAVPRHKDRFRDALDRVSQGLLFEGMEQAGPLIFEDLPLVRDR
                     LPGGAWAVVTAPRLAEERAESTRRECEALADALGWTGAETMAAPGEALEGMTRVDLSD
                     VTEGVDLGIEGWGPSLGDSGRVAGRAASLAGEGYTVAVTAQGHGSLDRAVEVLSSAGV
                     SPDSAISDGPVTGFVFPDGRVAVVGEDDLFGRRRRTHEAPRLAGRSAGAFALELSPGD
                     FVVHRVHGVARYAGMIRRRIGDAERDYLLLEYAKGDRLFVPADQVDVVSKYVGGESPR
                     VHRLGTNDWVRTKARVKRALRDMAGELVRLYSARMAAKGHAFPPDDVWQHELETAFPY
                     EETPDQLTAIDEVKRDMEASRPMDRLLCGDVGYGKTEVALRAAFKAATSGKQVAVLVP
                     TTLLAEQHFVTFAERYAPFPVRVAMLSRFLSAKEQREVLDGLASGEIDVVVGTHRLLG
                     ADVRFKDIGLVIVDEEQRFGVAHKERLKQLRLDVDVLTMTATPIPRTLEMALTGIRDM
                     SVVDTPPEDRQPVLTYVGSWEDDVALGAARRELRRGGQVFWVHNDTRTIDRRAGWLAE
                     RIPEARIGVAHGQMDEKVLEKTMLAFWNGEVDVLLTTTIIESGLDVPTANTLVIERAD
                     ALGLAQLYQLRGRVGRSAERAFAYLFFPPQRQLTEEAHARLAAIGRHTALGSGFQIAM
                     RDLEIRGAGNLLGAEQSGHIAAVGFDTYARLLQEAVAELQGEPVPEEAPVRIDLPIKA
                     YLPPDYLGQESLRLDLYREIAGARDEADLDRIRSATEDRFGPLPLEGEALIELTRLRI
                     VCERHGVEEVSTFRGQVRVRPFVPDVEDLPERTAWHAATSTFNLDPPALAGADLARWL
                     RDRLVEASGARTEPAASG"
     gene            9582..10334
                     /locus_tag="VF058_09510"
     CDS             9582..10334
                     /locus_tag="VF058_09510"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX6208576.1"
                     /translation="MAPTVESDSHPATDAVLRVGSDPSFQGWRLTDHSYTIGRDEASS
                     IRLGDPRVSRQHARLYRQAGQWWISDLGSANGTWVNGQQVEQSALSPGDRIQVGGTDL
                     ILTVPQARAAEGVVPRNDPTAAVNRGGPGTTFHIRDQAGDIDNIGRDQNIYVGSDAAS
                     LEELRKAGPALKALFAVAILLCVAGLVVFIGGMFFMDTTLPDPRNPGFIDDVLEGPET
                     PLNVIVGFGLFLTGFVLAVIASLILALKRRKE"
     gene            10339..10755
                     /locus_tag="VF058_09515"
     CDS             10339..10755
                     /locus_tag="VF058_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208577.1"
                     /translation="MVEDDSGGGATFNIGGQQGNITNVGRDMHVHGQQTNQALIASSE
                     ILTELAQVREQVRELDLPEEDAREVDQALTEAETELQQPEPDAGRVGGHIERATEILK
                     RVGGLATAGMALAGPLGKIGALLGPVGRSILRLLGV"
     gene            complement(10760..11929)
                     /locus_tag="VF058_09520"
     CDS             complement(10760..11929)
                     /locus_tag="VF058_09520"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6208578.1"
                     /translation="MTPAFLLIALSTLAYFVAEGALIPTVPRYVEGPLGGSEAAVGMV
                     VGAFAVTALLFRPWAGRLADRRGRRFVMLIGMVLFGSSVAGYVVAESIPVMVAMRLLT
                     GAGAAFFFVGAATAVTDLAPEARRGEAISFFSLSLYAGVGAGPVLGEVAVSASGFDLA
                     WWLIVAASVLAFVLALPVPDTRPPERDLGPLKIVHPAAVVPGLVILASIWGMAGFFAF
                     TPLYVDRLGLEDSRFVFLLFSGVVLLIRGFGAKLPDRLGPGRAARAALTASALALLVI
                     GTFESTPGLLIGTAILGVGVGLAFPALMSMTIANTKPAERGAAVGTFSAFVDLAFGVG
                     PLTLGYVAEGLGLRSVYFAAAGVALLGLLMLAVRRQRRRRGRRPEPEMPVPEMPA"
     gene            12018..14054
                     /locus_tag="VF058_09525"
     CDS             12018..14054
                     /locus_tag="VF058_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX6208579.1"
                     /translation="MQRILVGTDRSSSADRAVRWAADLASRFEAELVVLQVILPEEET
                     EIEEGARAQHALEELRLLAEEVAGPRGKARVVVDADPAAAICRAAAEEVVDSVVVGNY
                     GMAGRKRFLLGNIANRVSHSAPCSVIIANTVSPEDRVVHPTREEREAAETEPHLLPRA
                     ARIGRVMAKAGLRDLFRPGASDEDLRDRAGKLRTAFEELGPTFGKLGQVLATRPDLLP
                     QPFIHELSSLHSSVPPVSEEEIVAVMEQELGVPWEDVFESIEPEPLAAGTMAQVHVAT
                     LADGDEVVVKIQRPTARQDILQDLGLLQMFAEKTRDKKALRAVVDLPAIVEQLSESLQ
                     RELDFSVEAKNAERLREVLAPYPRLDVPRVYTEFSTERLLVMEAIRGVPVHEAPLGPE
                     RIEAARQLLESYYRQVLGDGFFHADPHPGNLLWADGKVWFLDVGMVGELGPEVRELLV
                     LLIMAFWQQDVAFLSDMVLMLTGEDQRQDLDLHAFQEELGHMVDRYRGLSLRDIQLGP
                     ILQEITEISVRHQVRLPASLALTGKALAQVQLIVSDLDPELDPFSVAGSFLVKGVGSR
                     VLSRLDPQKLFYEGQKLKVRASRMIETLERLAGSRPGQKLQVDFRGMDKLEDTVRATG
                     RRLSMAIGAGAAFIATSATAVAADVASWVPMTLGGVGGALTLGLAYDLIARRRR"
     gene            complement(14055..14441)
                     /gene="msrB"
                     /locus_tag="VF058_09530"
     CDS             complement(14055..14441)
                     /gene="msrB"
                     /locus_tag="VF058_09530"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004216835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX6208580.1"
                     /translation="MEKTDEEWRRELTPERYAVLRQQATEAPFTSELLQVKEDGTFRC
                     AACGTELFSSQTKYDSGTGWPSFYEPMAEDNVELRPDTSHGMMRTEVVCATCGGHLGH
                     VFDDGPEPTGLRYCINGCALEFDSEE"
BASE COUNT         2116 a         5340 c         5065 g         1922 t
ORIGIN      
        1 gatgcgaggg tcttccgggg gaaggtcggc cacgatggac gcagccacga tggacagcac
       61 cgcggcggag atgtcctgga cgctgccgaa cgtgccccgg tggctactga cgagggccac
      121 ggtgatcagg atcgccgcgg cggccacgag tccgaggccg atgccagccg gcaggtgctc
      181 ggccagcggc ccgctgaaga tgagagcggc gaaggaagtg gccacgacca cctcgaggac
      241 ccccaccacc acgccggccg tcagggtcgg gaacagactc gccccacgcc ccgatcgggg
      301 aggatgtccg tcatgtgccg tcgccgtcgc ggagccttcc gcggacgtcc ccatggtccg
      361 gtcccccttc agcccgagcg gcctccgcga tggtggcaca cgccccgcgc cgaagcgaga
      421 ggaggccggc cgccggacct ctacgccgac cgcaggtcga gccgccagtg gttaacgcgc
      481 agctggcgat cacggaaccg gttcgtgcgc tcgccgagca gcgtgaaccc tagggtccgg
      541 cacatcgcgt tcgaggccag gttcgagacc ggggggaacg cgtggagcac gtcccaccgc
      601 ccggtctccc gagcacggtc gagcgccatc cgcacccccg cgctccccac gcccttcccc
      661 tggtggtcgg ggagcaccat ccacccgatc tcggtgagta gctcgccgcc cacctcgtgc
      721 tcccagatgc tcacggtccc cgcggcggtc tcctcctcga ggacgatcac gaggacccac
      781 acctcgtcgg cctcggtggc ggccgcgtcc cgctcgagct tctcggccag gcccgccttg
      841 ggcaggggcc cgccgaggtg ctccatcatc cgggagtcgc agtggatcgc ctcgtagagc
      901 cagaggtcct cggtcgtgag gttcctcagc cgcacgggct cagcaggagg cgggaagggg
      961 ctggccgtcc tcggggaccg cgacgaaggc gatctcgccg ggccgcacca tccccaggca
     1021 ctggcgggcg atccgctcca ggtactcggg gtcccgcagc cgctccacgc ggcgttcgag
     1081 cgtgactcgc tcctcctcga gacgctggac ctgtcgctcc aggcctgcga tctcgctccg
     1141 ctggatcacg tactggcgaa gggggaacac gagccccagc agcaggacga acgagatggc
     1201 ggcgaggccg gccgcccggc ggctcagccg gggtcggggc aggacgtgac ccctcgcgct
     1261 catcgtccga acctccggta gaccgcggcc cccgcgtacc gggcggtgcc ccccagctcc
     1321 tcctcgatcc tgaggaggcg gttgtacttc gccgtcctct cgccgcgcga cggagcgccg
     1381 gccttgatct ggcccgcgtt cgtggccacg gcaagatcgg cgatcgtgga gtcctcggtc
     1441 tcgcccgatc ggtgggagat cacgacgccg aagctcgcgg ccaccgcggc gtccacgacg
     1501 tcgagcgtct cggtgaggct cccgatctgg ttcggcttga ccaggatcgc attgcacgag
     1561 gcctccgcga cggcgcgcga gagacgctcc gggttcgtca ccagcaggtc gtccccgacg
     1621 agctggatcc ggttgcccag caggctcgtg acggcagccc agccctccca gtcgttctcg
     1681 tcgagggggt cctcgatcga cacgatgggg aagtcggtca cgaggcggtc gtagtactcg
     1741 gccatctgct cggccgatcg ggcctcgcct tccacgtgat aggcgtcgtc ccggaagacc
     1801 tcgctggccg ccgcgtcgat cgcgatcgcc acgtcggacc ccggctcgag gccggccgcc
     1861 tcgatcgcgc ccaccagggc ctcgcaggcc gccgccgagc cggggaggtc gggcgcgaac
     1921 ccgccctcgt cccccacggc ggtggacagt cccttcccgt ggagctgctc cttcaacgtg
     1981 tggaagacct cggagcccca gcggatcgcc tccgagaacg atgcggcgcc gcgaggcgcc
     2041 agcatgaact cctggagctc gatcgcgttg tccgcgtgcg ccccgccgtt gatcacgttc
     2101 aagaggggca gggggaggag gtgcgcgttc ggcccgccga ggtaccggta cagcggcagg
     2161 cccgacgaga cggccgcggc cttggcgttc gccagcgaga cggcgaggat cgcgttggcc
     2221 ccgagcccgg acttgtcgat tgtcccgtcg aggtcgagca gcatgcggtc gacctcgcgc
     2281 tgatcgaggg cctccacatc ctggagcgcc ggctcgatgc gctcgtggat gttctcgacc
     2341 gccttcagga cgcccttgcc cttgaagcgg tcgccgccgt cgcgcagctc gagggcctcg
     2401 tgctcgccgg tggaggcgcc tgagggcacg gcggccaggc ccagcgagcc gtcggccagc
     2461 tcgacctcgg cctccacggt ggggttgccg cgggagtcga ggacctctcg cgcgcggatg
     2521 cgttcgatgg ggggcatggg catccgattc taggacggag agggccgccg aacacgcatc
     2581 tccgtcagac ggcgcgcgcg agcacgtgcc ggtaggcgaa cccctcgtcc tcgaggagct
     2641 gctccgcctc gccctcgatc gcgaagccgg cctctccgag ccacgtccgc acccggtcga
     2701 gagacgggta gtggtggtag ccgtgctcct cccagatgac ctcgccgtcc accagcggga
     2761 ggccgcggcg ccgtcctccc tcggttccgg cccggacctc ctccggagga gcgacctcga
     2821 ccgtgaggta gagggggccg cttccgcgga tcgcccggcg gaagcgacgc aggacctccg
     2881 gccagtcctc cggcgggatc atctccacgg cgtccacgca catcacgccg tcgaacgcgt
     2941 cgcggtaggg gaggtcctgg aggtcgtgcc gctccgtctc ggctttcggg aacttcgtct
     3001 tcgcctcgac gagagaggcg ccggcatgat ccacacccaa gaccgagcga ccgctctcca
     3061 ggaccatgcc gaggtacttg ccggtgccgc acgcggcatc gaggaccctc ccggcgggcg
     3121 ggaccatcga caggaaccgc tcgacgaagg ctcggtgcgg ctcgtcgatc tcgccccaga
     3181 gctggtcgta cgtgggagcg agggcgtcct cctctcgctc gttcacgcgc cggagctcga
     3241 cgatccaagc ggcccgatcc atgcgggtca tcgtgccact actgctggag gacggtgtcg
     3301 cgctcgacgt ccggctccac gctccccttc gcggcgagcg tccccacccc gatcagcgcg
     3361 agcgccacgc cgagccactg cacgatcgag aggatctccg agaacagcag gtaggcccag
     3421 agcacggtca gcatcggctg gagcagcagc atcaccgagg tctcgagcga gggcaggcgg
     3481 gggagggcga ccgtgatcag cagccagccg aagacctggc agatgagggc cagggcgagc
     3541 agccagccgt gggctggcca cgtggggacc accgagaagg cagggtcgaa gatcggggcc
     3601 aggatcgcca tcccgaggag cgcgccggtc gtggcctcca tcatcgggcc ggccggcggc
     3661 gacaggctcc ggttcgatcg gcggagcagg atcaggaagc cggtgtagcc cagcccggtc
     3721 agcacgcccc agaagacccc gcccagcggg ttgctcccga acgcgtcctc cctccccagc
     3781 cccgagatca gcagcacgcc caggaacacg gtcgggacga gcacaaaggc gagcctggtc
     3841 ggtcgctccc gcaggaagat ccaggcgtag atcccgacga agacgacctg ggtgttcccg
     3901 atcacggtgg ccaggcccgc gccgatgtcg ccgatcgccc ggtgccagaa cgagaagtcg
     3961 agggccagga agagccccgc gccgaaggcc atccaccgct cgcggggcgt gcgctcgtcc
     4021 cggcgacggg tgaccagcca tacgagcacg agggccggga ccgcgtaggc cgcccggaag
     4081 aaggccgcgg tgtccgggga gacgtcggcg agcctcacga agatggcgga gaacgagatg
     4141 ctgacgatgc cgaccagcga gaggaggtgg gtcacgcgcg ggcctcgcgg aaccgccgca
     4201 ggaggtcgtc ctcggcgagg tcctcgagcc gaacgccctc ctcgcgggcc cgctcggcca
     4261 tcgtctcgaa cctgatgacg aacttccgga tcgtggagcg gagggcgctc tcggcgtcca
     4321 cacccagctt ccgggccagg gccgtgacgg cgaagaggac gtcgcccacc tcctcctcga
     4381 gggcgtcctt ggcctcgccc tcgacgtctc gtccggcgtg ggccgcggcc gcctcctcga
     4441 gctcggcgag ctcctcgcgg accttctcca cggctccggc tagcgcggcc cagtcgaagc
     4501 cccggccgga ggcacgacgc tggaccttct gggcggcgat cagcgcgggg agggccttgg
     4561 ggacgccctc gtccacgacg cgctccccct tctcctcggc tttgatccgc tcccagttcg
     4621 cgtagacggc ctcgggggtg tcggcgtcca cctccgcgaa gacgtgggga tggcgccgga
     4681 tcagcttctc cacggtgacc tcggccacgt cgtcgacgtc gaaggcgccc tcgtcggccg
     4741 ccatctgcgc gtggaagatc gcctggagca ggacgtcgcc cagctcctcc ttgaggtcgt
     4801 cgggggtccc ctcctcgatc gcctcgatca gctcgtaggc ctcgtcgaga aggtggcggc
     4861 ggagcgacat gtgggtctgc gcgcggtccc aggggcagcc gtccggggcg cggaggcgag
     4921 cgacgacccg aacgagatcg aggagtcgct cgccgccgcg ccccgacggc acagggcccg
     4981 tggggacgtg gtcgagcgga tcgggggtgc cgtggacctc ggccacggcg cctatcctac
     5041 tgccgtgccg tcgagcacct cgcggtcgcc cgggctcccg cccgccgcgc tcagggcgtc
     5101 gtctgcggac ccggcagcgg cgtggtggct ctccgctcga ccacctcgcc cgtgtcctcg
     5161 tcgaacgtcc cgtaccgggg gttcacgcgg acggtcaggg agcggagccg ctcgagcatc
     5221 cactcccgga agacctcggg ccccagctcc tggagcagct gctccctcgc ctgctcgaag
     5281 gggatcgcct cgcgactgtg caccaggatc acgtggaacc cgaactgggt ctccaccggc
     5341 cccccgatcc ggccctccgg gagctccagc agcgcgtcgg cgaactccgg gacgtaggcg
     5401 ccggcggccc gggtgcccag gtcccccccg ttcgcccccg atccctggtc gtcggagagc
     5461 tcccgagcca gccgggcaaa gttcgagggc gtggcctcgg ccacgatccg gctcgcctcc
     5521 tcctcgctgc cgacgaggat gtgcgaggcg tgcacctcgg tgaactcccg ggcccgctcc
     5581 tcgtacgccg cctgcagccg ctcgtccgag gcctcgtcct cgaccaccgc ctgccccacg
     5641 tcgctgcgga tgagctgggc ccgcacgaac gcgcgcacgt cctgggcggt ggcgtccgag
     5701 cgtccgatct ggtcctggat cgcctgatcc tgcatcagct cctccacgcg ctggcggtcc
     5761 acctggatgc cctcctcgcg ggccctctcc tcgaccacct cgtgctggat caggaaggtc
     5821 agcagctgtc gctggagcgt ggcccgctcg acgtcgggat tggccacggc cgcctccgcg
     5881 aactgcgggt cggccatgat gaagtcgagc agcctgagga actcctcgtc ctcgatcctg
     5941 tggggcccga cgaccgcggc ggccggctcg aaggcggtgc acgccgagag gacgagcgag
     6001 gcggcggcga ggagactcag gaggcgcagg cgcacgggcc ggattctagc cggatgccgc
     6061 cggctccgtc cgggcgcccg aggcctccac gagccggtcg cgcagccagc gggcgaggtc
     6121 ggcgccggcc agggcgggcg gatccaggtt gaaggtgctt gtcgccgcgt gccaagcggt
     6181 gcgctcgggc aggtcctcca cgtccgggac gaagggccgg acccgcacct ggccccggaa
     6241 cgtcgagacc tcctccacgc cgtggcgctc gcagacgatc cggagccgcg tgagctcgat
     6301 gagggcctcg ccctcgagcg gcaggggccc gaaccggtcc tcggtagcgc tccggatccg
     6361 gtcgaggtcc gcttcgtccc gtgcccccgc gatctcccgg tagaggtcga gccgcagcga
     6421 ctcctggccc agatagtccg gggggagata ggccttgatc gggaggtcga tccggaccgg
     6481 ggcctcctcg ggcaccggct cgccctgcag ctcggccacg gcctcctgga ggagcctggc
     6541 gtacgtgtcg aatcccactg cggcgatgtg tcccgactgc tcggccccca gcaggttgcc
     6601 cgcgccgcgg atctcgaggt cgcgcatcgc gatctggaag cccgagccca gcgcggtgtg
     6661 ccgcccgatc gcggccaggc gggcgtgggc ctcctcggtg agctggcgct ggggcgggaa
     6721 gaagaggtag gcgaaggcgc gctcggccga gcgccccacg cggccccgga gctggtacag
     6781 ctgcgcgagg ccgagcgcgt cggcccgctc gatcacgagc gtgttggcgg tggggacgtc
     6841 cagccccgac tcgatgatcg tcgtcgtcag gaggacgtcg acctccccgt tccagaaagc
     6901 cagcatcgtc ttctccagga ccttctcgtc catctggccg tgggccacgc cgatccgcgc
     6961 ctcggggatc cgctccgcga gccaccccgc ccgccgatcg atcgtcctcg tgtcgttgtg
     7021 gacccagaag acctggcccc cccggcgcag ctcgcgacgg gcggcgccca gcgcgacgtc
     7081 gtcctcccag gacccgacgt acgtcagcac gggctgacgg tcctcgggcg gcgtgtccac
     7141 gacgctcatg tcgcggatgc ccgtcagcgc catctccagc gtccggggga tcggggtggc
     7201 cgtcatcgtc aggacgtcca cgtcgagacg gagctgcttc agccgctcct tgtgggccac
     7261 gccgaaccgc tgctcctcgt ccacgatcac gaggccgatg tccttgaagc gcacgtcggc
     7321 cccgagcagg cggtgggtcc cgaccacgac gtcgatctct ccggaggcca ggccgtccag
     7381 cacctcgcgc tgctccttcg ccgacaggaa gcgcgagagc atcgccacgc gcacggggaa
     7441 gggcgcgtac cgctcggcga aggtcacgaa gtgctgctcg gccaggaggg tggtcggtac
     7501 gagcaccgcc acctgcttcc ccgaggtggc cgccttgaac gccgcgcgca gggcgacctc
     7561 ggtcttcccg tagcccacgt cgccgcacag caggcggtcc atcggcctgg acgcctccat
     7621 gtcgcgcttc acctcgtcga tcgcggtgag ctgatcgggt gtctcctcgt acgggaaggc
     7681 cgtctcgagc tcgtgctgcc agacgtcgtc gggggggaac gcgtggccct tcgcggccat
     7741 ccgcgcggag tacaggcgaa cgagctcgcc cgccatgtcg cggagggccc gcttcacccg
     7801 ggccttcgtt cggacccagt cgttcgtccc gagtcggtgc acgcgcgggc tctcgccccc
     7861 cacgtacttg gagacgacgt cgacctggtc ggcggggacg aagagccgat cgcccttcgc
     7921 gtactccagg aggaggtagt cccgctcggc gtcgccgatc ctccgccgga tcatccccgc
     7981 gtaccgcgcc acgccgtgga cgcggtggac gacgaagtcg ccgggggaga gctcgagggc
     8041 gaaggcgccc gcggaacggc ccgccagccg cggcgcctcg tgcgtccggc ggcgcctgcc
     8101 gaagaggtcg tcctcaccca ccactgccac gcggccgtcg gggaagacga agccggtcac
     8161 cggcccgtcc gagatcgcgg agtcgggtga gaccccggcg ctcgacagca cctccaccgc
     8221 gcggtcgagg ctgccgtggc cctgggcggt gacggcgacc gtgtagccct ccccggccag
     8281 cgacgcggcg cggccggcca cccggcccga atcgcccagg gacggccccc acccctcgat
     8341 cccgagatcc acgccctcgg tgacgtcgga gaggtcgacc cgggtcatgc cctcgagcgc
     8401 ctcgccggga gcggccatcg tctcggcccc cgtccatccg agcgcgtcgg ccagggcctc
     8461 gcactcgcgg cgggtggact cggcgcgctc ctcggccagg cgcggagcgg tcacgacggc
     8521 ccaggccccg ccgggaaggc ggtcccgcac gagcgggagg tcctcgaaga tgagcgggcc
     8581 ggcctgctcc atcccctcga acagcaggcc ctgcgagacg cggtccagtg cgtcgcggaa
     8641 gcggtccttg tggcgaggca cggcctccag ggctcgctcc atgacctcgg gcgtggggag
     8701 gagctcgcgg accgggtcgg cctccatccg ggggagcctc ccggtcgagg tctgggtgga
     8761 ggccgagaac tcgcgcagcg actccacctc gtccccgaac agctcgatgc ggaccggtcg
     8821 cggggccgcc ccggggaaga cgtccacgat ccccccgcgc acggcgaact ccccgcggtg
     8881 ggtcacgagg tcggcccgcg tgtagcccag cgccaccagt cgatccgcca gctcgtcggg
     8941 ctcgatctcc tcgccctgct gcacgacgag cgggtccacc gagcccacat ggggcagcag
     9001 ccgctggacg acggccaccg cgggagcgac gagcacgaac ggaccagagg cctcccgcgc
     9061 tcgccggatc gcggcgtggc ggcgagccga ggtctccggc gatgggctga tcccctccac
     9121 cggcagggcc tcccaggcgg ggaggtgggc cgcgcgatcg gcgcccaggt acgcggccac
     9181 gtcgtgggcc aggggcgccg attcgcgcgg cccacccgtc accgcgagca ccggcccctc
     9241 cagcgcctcc gccagcgccg cgatcgcgta ggcgtgaccg gccgggtcca cccgcaccgg
     9301 gcccggccgc gcgaggaccg cctgcagcgc ctcgctctcg atcaggggtt ccagcagctt
     9361 ccgcacagca cgacggggcc tccgccgacc tcggcggggc cctcgatctc ctttccggtt
     9421 ccgtcccggc cggatgccgg gcacccagcg attctagtgc ggggcggggc gatcgggacg
     9481 ctcgaagagc ccttcggcgg cgtccgtcgc tctcgacacc gaccctttac agcacatggg
     9541 ccgaagcgca gaatcgacgg gtcgctcagg gagggagact ggtggccccc acggtcgagt
     9601 ccgactcgca tcccgccacc gacgcggtcc tccgcgtcgg atccgacccc tcgttccagg
     9661 ggtggcgtct gaccgatcac agctacacga tcgggcggga cgaggcgtcc tcgatccgcc
     9721 tcggcgatcc ccgcgtgtcg aggcagcacg cccgcctgta tcgccaagcg ggtcagtggt
     9781 ggatatccga cctgggctcg gccaacggga cgtgggtcaa cgggcagcag gtcgagcagt
     9841 cggcgctgtc gccgggcgac cgcatccagg tcgggggcac cgacctcatc ctgacggtgc
     9901 cccaagcccg agcggcggag ggcgtcgtcc cccgcaacga cccgacggcg gccgtcaacc
     9961 gcggcggccc gggcacgacg ttccacatca gggaccaggc cggcgatatc gacaacatcg
    10021 gccgggacca gaacatctac gtcggctccg acgccgccag cctcgaggag ctgcggaagg
    10081 cggggccggc cctcaaggcg ctcttcgcgg tcgccatcct cctgtgcgtc gccgggctcg
    10141 tcgtgttcat cggcgggatg ttcttcatgg acaccacgct gccggacccg cgaaaccccg
    10201 gcttcatcga cgacgtgctg gagggccccg agacccccct caacgtgatc gtcggattcg
    10261 ggctcttcct cacggggttc gtgctggcgg tgatcgcctc gctgatcctg gccctcaagc
    10321 gacggaagga gtagcggcat ggtcgaggac gactccggcg gcggagcaac cttcaacatc
    10381 ggaggacagc agggcaacat cacgaacgtc ggccgcgaca tgcacgtgca cgggcagcag
    10441 acgaaccagg ccctgatcgc ctcgtccgag atcctcacgg agctggcgca ggtacgggag
    10501 caggtgcgcg agctcgacct cccggaggag gacgcccgtg aggtggacca ggcgctgacc
    10561 gaggccgaga cggagctcca acagcccgag ccggacgccg gccgggtggg cggtcacatc
    10621 gagagggcaa ccgagatcct gaagcgggtg ggcggtctgg ccaccgccgg catggcgctg
    10681 gcggggccgc tggggaagat aggcgcgctg ctcgggccgg tgggacgctc gatcctgcgg
    10741 ctgctcggcg tctgatcgct caggcgggca tctcggggac cggcatctcg ggctccggcc
    10801 ggcgcccccg gcggcgccgc tgccgccgca ccgcgagcat caagaggccg agcagcgcca
    10861 cccccgccgc ggcgaagtac accgaccgca gccccagccc ctcggcaacg taccccagag
    10921 tcagcggccc caccccgaag gccaggtcca cgaacgcgga gaacgtcccc accgccgcgc
    10981 cccgctccgc gggcttcgtg ttggcgatcg tcatcgacat cagcgccgga aaggcgagcc
    11041 ccacgcccac gccgaggatc gccgtcccga taagcaggcc cggcgtcgac tcgaacgtcc
    11101 cgatcaccag gagcgccagc gccgacgccg tcagcgccgc gcgggccgct cggcccggcc
    11161 ccagccggtc gggcagcttc gccccgaagc cgcggatcag caggaccacc cccgagaaca
    11221 gcaggaagac gaaccgcgag tcctccagcc ccagccggtc cacgtacagc ggcgtgaagg
    11281 cgaagaaccc ggccatcccc cagatgctgg ccaggatcac gagacccggg acgaccgccg
    11341 ccgggtggac gatcttcaac ggcccgaggt cccgctcggg cggcctcgtg tcgggcaccg
    11401 gcagcgcgag gacgaaggcc agcaccgacg ccgccacgat cagccaccag gccaggtcga
    11461 agcccgacgc cgagacggcc acctctccca gcaccggacc ggcccccaca cccgcgtaca
    11521 gcgagagcga gaagaacgag atggcctccc cccgacgagc ctcgggggcg aggtcggtca
    11581 ccgcggtggc cgcgcccacg aagaagaacg cggcaccggc gccggtcagg agccgcatcg
    11641 cgaccatgac cgggatcgac tcggccacga cgtagccggc caccgaggag ccgaacagaa
    11701 ccatcccgat cagcatcacg aagcggcgac cgcgcctgtc ggccagccgg cccgcccacg
    11761 gccggaacag cagggcggtg accgcgaacg cacccaccac catccccacc gcggcctcgc
    11821 tcccgccgag cggcccctcc acgtaccgag gcacggtggg gatcagggcc ccctccgcca
    11881 cgaagtaggc cagcgtcgac agggcgatca gcaggaaggc cggcgtcacc agcctcggcc
    11941 tcgtcgcctc gcgcgtcacc cgtctccccc cacggccggg caggctagca cccccgccgc
    12001 ggtatcatcc gctcgccgtg cagcgcatcc tcgtgggcac cgaccgctcc tcctccgccg
    12061 accgggccgt ccgctgggcc gccgacctgg cctcccggtt cgaggcggag ctcgtcgtgc
    12121 tgcaggtgat cctcccggag gaggagaccg agatcgagga gggggcccgg gcccagcacg
    12181 cgctcgagga gctgcgcctg ctggcagagg aggtcgccgg cccccggggc aaggcccgcg
    12241 tggtggtgga cgccgacccg gccgcagcga tctgccgagc cgccgccgag gaggtggtgg
    12301 actcggtggt cgtgggcaac tacggcatgg ccggccggaa gcggttcctc ctgggcaaca
    12361 tcgccaaccg cgtctcccac agcgccccct gcagcgtgat catcgccaac accgtctctc
    12421 ccgaggaccg ggtcgtccac cccacccgcg aggagcggga ggcggccgag accgagcccc
    12481 acctcctccc ccgcgccgcg cggatcggca gggtcatggc caaggccggg ctccgcgacc
    12541 tcttccggcc cggcgcctcg gacgaggacc tccgcgaccg cgccgggaag ctacggaccg
    12601 cgttcgagga gctgggccca acgttcggga agctgggcca ggtgctcgcc accagaccgg
    12661 acctcctccc ccagcccttc atccacgagc tctcgagcct gcacagcagc gtcccgcccg
    12721 tctccgagga ggagatcgtc gcggtgatgg agcaggaact cggggtcccg tgggaggacg
    12781 tcttcgagag catcgagccc gagcccctgg ccgcggggac gatggcccag gttcacgtcg
    12841 cgacgctggc cgacggcgac gaggtggtcg ttaagatcca gcggccgacc gcgcgccagg
    12901 acatcctcca ggacctcggg cttctgcaga tgttcgcgga gaagacgcgg gacaagaagg
    12961 cgctccgggc cgtcgtggat ctcccggcga tcgtggagca gctctcggag tcgctccagc
    13021 gcgagctcga cttctcggtc gaggcgaaga acgccgagcg gctgcgcgag gtgctggcgc
    13081 cctacccgag gctcgacgtc ccccgcgtct acacggagtt ctcgaccgag cggctgctgg
    13141 tcatggaggc gatccgcggg gtcccggtcc acgaggcgcc cctcgggccc gagcgcatcg
    13201 aggccgcccg tcagctcctc gagtcctact accggcaggt tctcggggac ggcttcttcc
    13261 acgccgaccc gcacccgggg aacctcctgt gggccgacgg caaggtctgg ttcctggacg
    13321 tggggatggt cggggagctc ggccccgagg tccgcgagct cctggtgctg ctgatcatgg
    13381 cgttctggca gcaggacgtg gcgttcctct cggacatggt tctgatgctg acgggcgagg
    13441 accagcgcca ggacctggac ctgcacgcgt tccaggaaga gctggggcac atggtggacc
    13501 gctaccgcgg gctctccctg cgcgacatcc agctcggacc gatcctccag gagatcaccg
    13561 agatctccgt ccgccaccag gtgcggcttc cggcctcgct ggcgctcacc gggaaggccc
    13621 tggcccaggt ccagctgatc gtctcggacc tcgaccccga gctcgatccg ttctcggtgg
    13681 ccggctcgtt cctcgtcaag ggcgtgggct cgagggtgct gagccggctg gatccccaga
    13741 agctcttcta cgaaggacag aagctgaagg tgcgggcctc gcggatgatc gagacgctgg
    13801 agcggctggc cgggtcgagg ccgggccaga agctccaggt cgacttccgg gggatggaca
    13861 agctcgagga cacggtgcgc gccaccggcc ggcggctctc gatggcgatc ggggcgggag
    13921 cggccttcat cgccacgagc gccaccgccg tagcggccga cgtggcgtcg tgggtcccga
    13981 tgacgctggg gggcgtcggc ggcgcgctga ccttgggact ggcgtacgac ctgatcgcgc
    14041 gccgccgccg ctgactactc ctccgagtcg aactccagag cgcagccgtt gatgcagtag
    14101 cgcagccccg tgggctcggg gccgtcgtcg aagacgtggc ccaggtgtcc gccgcaggtc
    14161 gcgcagacca cctcggtccg catcatcccg tgcgaggtgt cgggccggag ctcgacgttg
    14221 tcctcggcca tcggctcgta gaaggagggc cagccggtgc ccgagtcgta cttcgtctgt
    14281 gacgagaaga gctcggtccc gcacgccgcg cagcggaacg tgccgtcctc cttcacctgc
    14341 agcagctcac tcgtgaacgg cgcctcggtg gcctgctggc gcaggaccgc gtaccgctcc
    14401 ggggtcagct cccggcgcca ctcctcgtcc gtcttctcca ccg
//
LOCUS       DASUTW010000282        12264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1131072, whole genome shotgun sequence.
ACCESSION   DASUTW010000282 DASUTW010000000
VERSION     DASUTW010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12264
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131072"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /gene="ftsL"
                     /locus_tag="VF058_09535"
     CDS             <1..382
                     /gene="ftsL"
                     /locus_tag="VF058_09535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEX6208581.1"
                     /translation="RTRRPRPAGRRAPARHARKRLPFLLFSLVVVSAMIVLLVSAQAI
                     VAQSAFRITELRERVDVIENEHAQLRLEAARLSSPERIMRAARDAGLVLPDRVEILAV
                     SGKGASRIRSHLGTLASADLGGEG"
     gene            379..2064
                     /locus_tag="VF058_09540"
     CDS             379..2064
                     /locus_tag="VF058_09540"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX6208582.1"
                     /translation="MSRRPPAGRVRLLLAVLVLGFSGIATRVSVLHLRDAEAYEALAH
                     NQRVREVVLPARRGSIFDRNGHDLAISLDARAIYANPLVIDRPWKVAGRIAPLAGERR
                     RAIARALEREQPFVYLARRVDLAHAEEVEALGIPGVGLLEESKRYYPSGTLAAQVLGF
                     VGVDGLGLEGIELAYDEVLGGTPGTMVVEQDPEGRPIPQGDAWIEEPVPGESLVLTID
                     RDLQWQAQRALQKAVKRNGAKGGTVVVLDPSTGDVLAMATYPWFDANEYTDVPQDVLR
                     NRAVSDVYEPGSVNKVITAAAALEEGVVGPREAIDVPDAYQLGDKLFRDVHPHAPQAM
                     TLSDIIAESSNVGTIKVAERLGRDRLATWLTRFGFGRRTGIDFPGEAEGILLDRDDWW
                     VTSMGTIPMGQGIAVTPLQMASVYATVANDGVRIRPRLVRGTIRPGQRFEPSPPGPRR
                     RVIGKDTARALREMLVRVVEEGTGDLARVEGYEVGGKTGTARKPLEDALGYSDEYVAS
                     FIGFAPADEPRIVVAAMLDEPDTVYGGVAAAPLFREVARFALADLQVPPTEPG"
     gene            2097..3560
                     /locus_tag="VF058_09545"
     CDS             2097..3560
                     /locus_tag="VF058_09545"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX6208583.1"
                     /translation="MASTPQLSDLLAGLPNADVRGDARTPVAGLAYQADAVSPGGLFF
                     CVPGGRHDGHDFADRALASGASVLVVERSLDLEATQVLVPSVREAMGPIAAAFHGRPA
                     ERISLVGITGTNGKTTTTYLLEGVFRAAGLTPGVIGTTGVRIDGRPVPQARTTPEAPD
                     LHAMLARMVDEGVGAVAMEVSSHGLHQHRVGGARFACAVFTNLSQDHLDYHGTLDEYF
                     EAKARLFTPRMSDRAAVNVDDAMGRRLLDTGLPTITYGRDEDADLRAVDEEVGAGGVR
                     FVADGLEVRSPLRGAFNVHNALAALAASRQLGIEDEEAAAGIASVGHVPGRLEPVDEG
                     QGFLVLVDYAHTPDSLEGALLAARPLTGDGGRVIAVFGCGGDRDRGKRPLMGEAATRR
                     ADLTVITSDNPRSEEPVEIIREIEPGARRGGGAFVVEPDRRTAIRLALEGAGEGDVVV
                     IAGKGHETGQEFADHTIPFDDRVVAGEELRVLMGDGS"
     gene            3557..4930
                     /gene="murF"
                     /locus_tag="VF058_09550"
     CDS             3557..4930
                     /gene="murF"
                     /locus_tag="VF058_09550"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017565734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX6208584.1"
                     /translation="MRPRTLSSVAELTRGRLAGADATVRGATADSRDVRPGELFVALR
                     GERTDGHRFVDAAFEAGAAGALVEAEGEYAGPVVVTPESARGLLELAADEREARDAHV
                     VAITGSTGKTSVKDLTAAVLASRHRVHASPRSFNTEVGVPVTLLRAPEDAEAIVLEMG
                     ARGRGHIALLAGVARPEVGIVTNVGAAHLEMFGSRDAVAEAKAELVEHLEPDGAAVLN
                     ADDPVVRGFGDRTAARVLLYGQDPDADVRGEDLRLDEQGRASFTLRTPEGSERVELSI
                     VGEHMASNALAAAAAGAALGLSPGECAAALKDARLSAWRMEVAETPHGVRVLNDAYNA
                     NPSSMAAGLKAAVWMSRGGRCIAVLGWMAELGASSDEEHERVGELVARLGVDHLVTVG
                     EHAARIAVGAAREGVEPGRITEVRDADEAAAEVLRIAEAGDLVFLKGSRVVGLERVAS
                     LLMEGGA"
     gene            4927..5985
                     /gene="mraY"
                     /locus_tag="VF058_09555"
     CDS             4927..5985
                     /gene="mraY"
                     /locus_tag="VF058_09555"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX6208585.1"
                     /translation="MTAILLAAAVGLIVTLLGTPLAISVFRFLGWGQRVREPTDPHYP
                     AHGAKIGTPTMGGLVILAGVFLGFVTTRLVTGGLSATGLLLMGVALSLGALGYVDDLL
                     KVKGHHSLGLGKTSKLLVQAVVAVLFAIGLDRLTGVSTELSFIRETGIDLGIGFLIWV
                     FLMVTATSNAVNLTDGLDGLASGSVALIMGAYVIVSFWQVRHACVLLDVPGCYDITPQ
                     GSLDAAVVAAATMGAAAGFLWWNAAPARIFMGDTGSLALGGLIAAMAIVTHTQLLLVI
                     LGGLFVLETASVILQVVSFRVFRRRLFRMAPIHHHFEVLGWPEFTVIVRFWIVAGLAV
                     AFGLGLFYADFLARGGLL"
     gene            5982..7328
                     /gene="murD"
                     /locus_tag="VF058_09560"
     CDS             5982..7328
                     /gene="murD"
                     /locus_tag="VF058_09560"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX6208586.1"
                     /translation="MTLDVRDRTVVVVGLAASGLAAVRVLRGEGAVVRVTEARPREEV
                     EGEVGAAEAAGAEVLAGGHAPEHLRGADLVVTSPGVPEGAPVLAWALDRGIRVWSEIE
                     LGARLSAAPYVGVTGTNGKTTTTEMVAAALRADGRDAVACGNAGHPFSLAATEGHDVL
                     AVEVSSFQLRFHERFNPRVSVLLNVAPDHLDWHGSLEAYGAAKARIYTCQGEGDLHVG
                     NADDPVAAEISRSAPCPVAWTTLGEPDEGQVGYVADRLVSRLDAERDLGPVRPASPPH
                     MADAAAAAAAALGFGAAPEAVGEALRGFRPLAHRGEVVARRGDVVFVDDSKATNPHAA
                     LASIAGWDRVALIAGGRAKGVDLAPLTEAAPRLLGVVAIGEAAGEVARAFEGIRPVAR
                     ADSIDEAVGLAAEMAAPAGTVLLAPACASQDMFRDYDERGRLFAAAARALAEREAG"
     gene            7325..8518
                     /gene="ftsW"
                     /locus_tag="VF058_09565"
     CDS             7325..8518
                     /gene="ftsW"
                     /locus_tag="VF058_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX6208587.1"
                     /translation="MSTAAARKRRPRHLRLVEPEERASRSPSRAERNDRHVTMLVVST
                     AALTLLGLIMVLSASSVEAYARFGSSFLFFNRQVVYAVVGSLAMAVTMRMRHRAWERL
                     AGPMLLLSVVLLAMVLLPGFGTVAGGSARWIELGPVTIQPSEFAKLSVVAFGAALLSR
                     RWHRIEDLRQLAVPLLPVAGVVCGLILLQPDLGTAVIIVTSVFVLLVAAGARMRHLLV
                     GAFATGTVGLGLIYVEGYRWSRFVSYLDPWADPQGSGYQVIQSLIAITSGGPFGVGLG
                     ASRQKWQYVPNAHTDFIYSIIGEELGLAGMLVVLGLFAVLVYAGIRVAIAAPDAFSRL
                     LAAGIVGWIGGQALINLGAVTGLLPITGVPLPFVSFGGSSLVVSLAAVGILASIARRG
                     RAERG"
     gene            8522..9616
                     /gene="murG"
                     /locus_tag="VF058_09570"
     CDS             8522..9616
                     /gene="murG"
                     /locus_tag="VF058_09570"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX6208588.1"
                     /translation="MRVVLAAGGTAGHIFPGLALARRLRDGHGAQVLFVGREGGQEAV
                     LVPEAGFRLATVESRPFVRRLSPAALAAPVAALRASRRARGFLRTADVAVGMGGYTSV
                     PVALAAWRERVPLVLHEQNAVPGLANRVAARWARAVAVSFSEAASRFPRGARTVVTGN
                     PVRQSIARIHQERDALAKEAREELGLEESRRTVVVFGGSQGALHLDRAAADAARLLSG
                     SDLQLLIVTGPAHLEEVEGRLPGGPLPVRAVPFVDRMELVYAVADLAVARAGAGTVAE
                     LSVAGVPALLVPYPYATGNHQEANARAMQRAGGAAVLLDDQLTAEVLAERVIEMLEPT
                     RLAAMAERAASFGRPGAAEALARVVVEAAR"
     gene            9613..11037
                     /gene="murC"
                     /locus_tag="VF058_09575"
     CDS             9613..11037
                     /gene="murC"
                     /locus_tag="VF058_09575"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009883147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX6208589.1"
                     /translation="MKDYEPRPGTIPTLEIPRLDLRRVHLVGVGGAGMSGIARLLLAR
                     GIAVSGSDLKDSPGLALLRGAGAEVTVGHRPENVGSAEAVVVSTAIGRDNPEVVAARR
                     RGLPVYARAQVLAAMMAERRGIAIAGTHGKTTTTSMTAVIFERAGLDPTYVVGGDLNE
                     SGSNARSGDGDYFIAEADESDGSFLLLDPEIGVVTNVEPDHLDFYRGGEDVEAAFAAF
                     CRRADRLVACGDDPGVRRVLDAAPSEVVTYGMGEGNDVRLGSVETMNGEGRGVVVVDG
                     ERVEVSLSIVGRHHLLNATAAMLTARAVGVPLESAAEALRSFTGVRRRFEPRGDARGA
                     RFVDDYAHHPTEITATLEAARSTGPARLVAVFQPHRYTRTEALWRPLGGSLATADLVV
                     LTDVYGAGEMPVPGVTGKLLVDSLLDRAPRARVIYLPSRADVAPFLAGEIRNGDLVLT
                     LGAGDVTMVADETLSRIREANGGG"
     gene            11027..11965
                     /gene="murB"
                     /locus_tag="VF058_09580"
     CDS             11027..11965
                     /gene="murB"
                     /locus_tag="VF058_09580"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX6208590.1"
                     /translation="MAADAGRDAARILTEVAGDRVRRDFPLAPLTSFRLGGPALVYLE
                     AWSQEDLAALGRALEETGLPLLVIGKGSNLLISDEGFPGIVVRLGKGFRWAGREELRI
                     TAGGAMPLPAVANVALAHSLAGLEFGVAIPASLGGSVRMNAGAHHRSLDEVLASVEIY
                     DARAREPRIIPAIEMGFSYRDSRLPELSIVIAATAELRADDPAAIRARMDEARRWRRE
                     TQPLAEPNCGSVFKNPEGDHAARLIDRAGAKGLRLGGARVSEKHANFFIAGEGATASD
                     VRALIEEVRRRVHDAAGVWLEPEVHLAGSFDEEGAA"
     gene            11962..>12264
                     /locus_tag="VF058_09585"
     CDS             11962..>12264
                     /locus_tag="VF058_09585"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX6208591.1"
                     /translation="MTRRRNRAGIVVLAAVLLGILVGTVALRSPLFEVRRVEVRGAGR
                     LAPSEVRVLVEGARGRNLWLVPLDEIARSVSAHPWIARAEVGRRLPSTLVVEVEERI"
BASE COUNT         1500 a         4269 c         4739 g         1756 t
ORIGIN      
        1 aaggacccgc cggccccgcc cggccgggcg gcgggcgccg gcgcgccacg cccgcaagcg
       61 cctcccgttc ctcctcttct ccctcgtcgt cgtctccgcg atgatcgtgc tcctcgtcag
      121 cgcccaggcc atcgtggcgc agtcggcctt ccgcatcacc gagctccggg agcgggtgga
      181 cgtgatcgag aacgagcacg cgcagctccg gctcgaggcg gcccggctct cgtcgcccga
      241 gcgcatcatg cgtgcggccc gcgatgcggg actcgtcctt ccggaccgcg tcgagatcct
      301 cgccgtctct gggaaggggg cgagcaggat ccgctcgcac ctgggaaccc tggccagcgc
      361 ggatctcgga ggggagggat gagccgccgg cccccggcag gccgcgtccg actcctgctg
      421 gcggtcctgg tgctcgggtt ctcggggatc gccacgaggg tctccgtcct ccacctgcgg
      481 gacgcagagg cctacgaagc cctcgcccac aaccagcgtg tccgcgaggt ggtcctgccg
      541 gcccggcgcg ggtcgatctt cgaccggaac gggcacgacc tcgccatctc gctcgacgcc
      601 agggcgatct acgcgaatcc cctggtgatc gaccggccct ggaaggtagc cggccgcatc
      661 gcccccctgg cgggcgagcg tcgccgggcc atcgcccggg ccctggagcg cgagcagccc
      721 ttcgtgtacc tggcccgacg cgtggatctc gcccacgccg aggaggtgga ggcgctgggg
      781 atcccggggg tgggcctcct cgaggagagc aagcgctact acccgtcggg gacgctggcc
      841 gcgcaggtgc tcggcttcgt cggcgtggac gggctcggcc tggagggcat cgagctcgcc
      901 tacgacgagg tcctgggcgg cactccgggc acgatggtcg tcgagcagga ccccgagggg
      961 cgaccgatcc cccaggggga cgcctggatc gaggagccgg tccccggcga gtcgctcgtg
     1021 ctcacgatcg accgggacct ccagtggcag gctcagcgcg ccctccagaa ggccgtcaag
     1081 cgcaacgggg cgaagggcgg caccgtcgtg gtcctcgatc cctccacggg agacgtgctg
     1141 gcgatggcga catacccgtg gttcgacgcc aacgagtaca ccgacgtccc tcaggacgtc
     1201 ctgcgcaacc gcgccgtcag cgacgtctac gagccgggct cggtgaacaa ggtgatcacc
     1261 gcggcggcgg cgctggagga gggtgtcgtc gggccccgcg aggcgatcga cgtgcccgac
     1321 gcctaccagc tcggggacaa gctgttccgt gacgtccacc cgcacgcccc gcaggcgatg
     1381 accctgtcgg acatcatcgc ggagtcgtcg aacgtgggga cgatcaaggt ggccgagcgg
     1441 ctggggcggg atcgcctggc gacctggctc acgaggttcg gcttcgggcg gaggaccggg
     1501 atcgacttcc ccggcgaggc cgagggcatc ctgctcgatc gcgacgactg gtgggtgacg
     1561 agcatgggca cgatcccgat ggggcagggg atcgccgtca cgccgctcca gatggcatcg
     1621 gtctacgcca cggtggccaa cgacggcgtg cggatccgcc cccgcctcgt gcgggggacg
     1681 atccgaccgg gccagcggtt cgagccctcc cctcccgggc cacggcgtcg cgtgatcggc
     1741 aaggacacgg cccgggccct ccgagagatg ctcgtccggg tggtcgagga ggggaccggc
     1801 gacctggctc gcgtggaggg gtacgaggtg gggggcaaga cggggacggc gcgcaagccc
     1861 ctcgaggacg ccctgggcta cagcgacgag tacgtcgcct cgttcatcgg cttcgccccc
     1921 gccgacgagc cgaggatcgt ggtcgcggcg atgctggacg agcccgatac ggtctacggc
     1981 ggcgtggccg ccgccccgct cttccgcgag gtggcccggt tcgccctggc ggacctccag
     2041 gtccctccca cggagccggg atgatggtcc cggtgctagg atttcgtcgg cccacggtgg
     2101 catcgacgcc ccagctctcc gacctcctcg ccgggctccc caacgcggac gtgcgcggcg
     2161 atgcgaggac ccccgtcgcc gggctggcgt accaggccga tgcggtctct cccggcggcc
     2221 tgttcttctg cgtcccgggc gggcggcacg acggccacga cttcgccgat cgggcgctgg
     2281 cgagcggcgc ctcggtcctg gtggtggagc gatcgctcga cctcgaggcg acccaggtcc
     2341 tggttccctc ggtgagggag gcgatgggcc cgatcgccgc ggccttccac gggaggcccg
     2401 ccgagcggat ctcgctcgtg gggatcaccg gcacgaacgg gaagacgacg acgacgtacc
     2461 tgctggaggg cgtgttccgc gccgcgggcc tgacccccgg cgtgatcggg acgaccggcg
     2521 tgcggatcga cggccggccc gtcccccagg ccaggaccac cccggaggcc cccgacctcc
     2581 acgcgatgct ggctcggatg gtggacgaag gggtcggggc cgtggccatg gaggtctcct
     2641 cgcacgggct ccaccagcac cgggtgggcg gggcgcggtt cgcctgcgcg gtgttcacga
     2701 acctgtccca ggaccatctc gactaccacg ggaccctcga cgagtacttc gaggcgaagg
     2761 cccggctgtt cacgccccgg atgagcgatc gggcggcggt gaacgtcgac gacgcgatgg
     2821 gccggcggct gctcgacacg ggcctcccga cgatcaccta cggacgggac gaggatgccg
     2881 acctgcgggc cgtggacgag gaggtcgggg ccggcggcgt gcggttcgtg gcggacggcc
     2941 tcgaggtgcg ctcgccgctc cggggggcct tcaacgtcca caacgccctc gcggcgctgg
     3001 cggcctcccg gcagctcggg atcgaagacg aggaggccgc ggcgggaatc gcctccgtcg
     3061 gccacgtgcc cggccgcctg gagcccgtgg acgagggcca gggcttcctg gtgctcgtgg
     3121 actacgcgca cacgcccgac agcctcgaag gcgccctcct cgcggcccgc ccgctgaccg
     3181 gggacggcgg ccgcgtcatc gcggtgttcg gatgcggcgg ggaccgggac cgggggaagc
     3241 gcccgctgat gggggaggcc gccacgcggc gggcggacct gaccgtgatc accagcgaca
     3301 accccaggtc ggaggagccg gtggagatca tccgcgagat cgagcccggc gcgcggcgcg
     3361 gaggcggcgc cttcgtcgtg gagcccgacc ggcggacggc gatccggctg gcgctggagg
     3421 gggccggcga aggggacgtc gtcgtcatcg ccgggaaggg tcacgagacc gggcaggagt
     3481 tcgccgacca cacgatcccc ttcgacgacc gggttgtcgc aggcgaggag ctccgggtcc
     3541 tgatggggga cgggtcgtga ggcctcgcac cctctcctcg gtggccgagc tcacgagggg
     3601 gcggctcgcc ggcgcggacg ccaccgtgcg gggcgcgacg gccgacagcc gcgacgtccg
     3661 gcccggcgag ctcttcgtcg ccctgcgcgg cgagcgaacc gacgggcacc ggttcgtgga
     3721 cgcggctttc gaggccggcg cggcaggggc cctggtggag gcggagggtg agtatgccgg
     3781 ccccgtcgtc gtcacgccgg agtcggcgag gggactcctc gagctcgccg cagacgagcg
     3841 ggaggcccgg gacgcgcacg tcgtggcgat cactggctcg accggcaaga cctcggtcaa
     3901 ggatctcacg gccgccgtgc tggcttcgcg gcaccgcgtg cacgcgagcc cgcgctcgtt
     3961 caacaccgag gtgggcgtcc cggtgacgct gctgagggcc cccgaggacg cggaggccat
     4021 cgtcctggag atgggcgcgc ggggccgggg gcacatcgcg ctcctggccg gcgtggcccg
     4081 tcccgaggtg ggtatcgtca cgaatgtcgg ggcggcgcac ctcgagatgt tcgggagccg
     4141 cgacgcggtg gccgaggcca aggccgagct cgtggagcac ctcgagccgg acggcgcggc
     4201 cgtcctgaac gccgacgacc ccgtggtccg cggcttcggg gaccggaccg cggcccgcgt
     4261 cctcctgtac ggccaggatc ccgacgccga cgtccggggc gaggacctga gactcgacga
     4321 gcaggggcgg gcctcgttca ccctgcgcac cccggaaggg agcgagcggg tggagctgtc
     4381 gatcgtgggc gagcacatgg cgtcgaacgc gctggccgcc gcggctgcgg gggcggcgct
     4441 ggggctctcg ccgggggagt gcgccgccgc cctcaaggac gcccgcttgt cggcgtggcg
     4501 gatggaggtt gccgagaccc cccacggcgt acgagtcctc aacgacgcgt acaacgccaa
     4561 cccttcgtcc atggccgcgg gcctgaaggc ggccgtctgg atgtcgcgcg gcgggcggtg
     4621 catcgcggtc ctcggctgga tggccgagct cggcgcgtcc tcggacgagg agcacgagcg
     4681 ggtgggcgag ctcgtggccc gcctcggcgt ggatcacctc gtcacggtgg gcgagcatgc
     4741 ggcgaggatc gccgtcggcg cggccaggga gggcgtggag ccgggccgca tcaccgaggt
     4801 ccgtgacgcg gacgaggcgg cggccgaggt cctccggatc gccgaggcag gcgaccttgt
     4861 gttcctgaag ggctcgcggg tggtcggcct ggagcgcgtc gcctcgctcc tgatggaggg
     4921 cggcgcgtga cggcgatcct cctggccgcg gcggtggggc tgatcgtcac gctgctgggg
     4981 accccgctcg cgatcagcgt cttccgcttc ctgggatggg gccagcgggt acgggagccg
     5041 accgatcccc actaccccgc gcacggggcc aagatcggga cgcccacgat gggcgggctg
     5101 gtgatcctcg cgggcgtgtt cctggggttc gtcaccacga ggctcgtgac cggagggctg
     5161 agcgccaccg gcctcctcct gatgggcgtg gccctctcgc tcggcgccct ggggtacgtc
     5221 gatgacctcc tgaaggtgaa gggccaccac tcgctcggcc tggggaagac ctccaagctg
     5281 ttggtccagg cggtcgtggc ggtgctcttc gccatcggcc tggaccggct caccggggtc
     5341 tccaccgagc tctcgttcat ccgggagacg gggatcgacc tcgggatcgg cttcctgatc
     5401 tgggtcttcc tcatggtgac ggcgacctcc aacgccgtga acctcaccga cggcctggac
     5461 gggctggcgt cggggtcggt ggcgctgatc atgggagcct acgtgatcgt ctcgttctgg
     5521 caggtccggc acgcgtgcgt cctcctcgac gtgccgggct gctacgacat cacccctcaa
     5581 ggctcgctcg acgccgcggt ggtggccgcg gccaccatgg gggccgcggc cggcttcctc
     5641 tggtggaacg cggcgcccgc tcggatcttc atgggggaca cgggatcgct cgccctcggg
     5701 ggcctgatcg ccgccatggc gatcgtgacc cacacccagc tcctcctcgt gatcctgggc
     5761 ggcctcttcg tcctggagac ggcttccgtg atcctgcagg tcgtgtcgtt ccgggtgttc
     5821 cggcggcggc tcttccgcat ggcgccgatc catcaccact tcgaggtcct gggctggccc
     5881 gagttcaccg tcatcgttcg gttctggatc gtggcggggc tggcggtcgc gttcggcctt
     5941 gggctcttct acgccgactt cctggcccgg ggaggcctac tgtgacgctc gacgtccggg
     6001 accggacggt cgtcgtggtg ggcctggccg cgagcggcct ggcggcggtc cgggtgctcc
     6061 gaggcgaggg cgcggttgtg cgggtgaccg aggctcgccc tcgcgaggag gtcgaggggg
     6121 aggtcggcgc cgcggaggcc gcgggagcgg aggtcctcgc gggcggccac gcgcccgagc
     6181 acctgcgcgg cgccgacctc gttgtgacga gccccggcgt gcccgaggga gccccggtcc
     6241 tggcatgggc cctcgatcgc gggatccgcg tctggagcga gatcgagctg ggggcccggc
     6301 tgtccgccgc cccctacgtc ggggtgacgg ggacgaacgg gaagaccacc accaccgaga
     6361 tggtcgccgc cgcgctccgc gccgacgggc gggacgcggt ggcgtgcggg aacgcgggcc
     6421 atcccttctc cctggccgcg accgaagggc acgacgtcct ggcggtcgag gtgtcctcgt
     6481 tccagctgcg cttccatgag cgcttcaatc ccagggtctc ggtgctgctc aacgtggcgc
     6541 ccgatcacct cgactggcac gggagcctgg aggcgtatgg ggcggcgaag gcccggatct
     6601 acacgtgcca gggcgagggg gacctgcacg tcgggaacgc cgacgacccc gtggccgccg
     6661 agatctcccg atccgccccc tgcccggtgg cgtggacgac gctgggcgag ccggacgagg
     6721 gccaggtggg ctacgtcgcc gaccggctcg tgtcccggct ggacgccgag cgcgacctcg
     6781 ggcccgtccg tccggcgagc cctccacata tggccgacgc ggcggccgca gccgcggcgg
     6841 cgctcggctt cggggcggcg ccggaagccg tgggggaggc cctccgaggc ttccgtccgc
     6901 tggcccaccg cggcgaggtc gtggcccgcc gcggcgacgt cgtcttcgtg gacgactcca
     6961 aggccacgaa cccgcacgcg gccctggcct cgatcgcggg gtgggaccgt gtggccctga
     7021 tcgccggagg gagggccaag ggcgtggacc tcgcgccgct gacggaggcg gcgccgcgcc
     7081 tcctcggggt cgttgccatc ggcgaggccg caggggaggt ggcccgcgcg ttcgagggga
     7141 tccgccccgt ggcccgcgcc gactcgatcg acgaggcggt ggggctcgcc gccgagatgg
     7201 cggcgcccgc cgggaccgtc ctgctggccc ccgcctgcgc cagccaggac atgttcaggg
     7261 actacgacga gcgcgggagg ctgttcgccg ccgcggcccg agcgctcgcc gagagggagg
     7321 cgggatgagc accgccgcgg cccggaagcg tcggccccgt cacctccgac tcgtcgagcc
     7381 ggaggagcgg gcctcccgca gcccgtcccg cgccgagcgc aacgaccgac acgtcacgat
     7441 gctcgtggtg agcacggccg cgctcaccct cctcggtctg atcatggtgc tctcggcctc
     7501 ctctgtggag gcgtacgcgc ggttcgggtc gagcttcctc ttcttcaacc gacaggtcgt
     7561 ctacgccgtc gtcggctctc tggcgatggc cgtgacgatg cggatgcgcc atcgggcctg
     7621 ggagcggctg gcgggcccga tgctgcttct gtcggtcgtg ctcctggcca tggtcctcct
     7681 gccggggttc gggacggtgg ccggcggttc ggctcgctgg atcgagctcg ggcccgtgac
     7741 gatccagccg tcggagttcg ccaagctctc cgtggtcgcg ttcggggccg ccctgctgtc
     7801 gcgccggtgg cataggatcg aggatctccg tcagctggca gtgcccctgc tcccggtcgc
     7861 cggggtcgtg tgcgggctga tcctcctcca gcccgacctg gggacggccg tcatcatcgt
     7921 gacctcggtc ttcgtcctgc tggtggcggc gggcgcacgg atgcgccacc tgctcgtcgg
     7981 cgcgttcgcc acgggcacgg tcgggctcgg cctgatctac gtggaggggt accgctggtc
     8041 gcgattcgtc tcgtacctcg atccgtgggc cgatccccag ggcagcgggt accaggtgat
     8101 ccagtcgctg atcgcgatca cctcgggggg cccgttcggg gtggggctgg gcgcctcccg
     8161 ccagaagtgg cagtacgtcc cgaacgcgca caccgacttc atctactcga tcatcgggga
     8221 ggagctcggg ctcgcgggga tgctcgtcgt cctggggctc ttcgccgtcc tcgtgtacgc
     8281 ggggatccgc gtggccatcg cggcgccgga cgccttctcg cgcctgctgg ccgcggggat
     8341 cgtgggctgg atcggcggcc aagcgctcat caacctgggg gccgtgaccg gtctcctccc
     8401 gatcaccggc gtgccgctgc cgttcgtctc cttcggcggg tcctcgctcg tcgtctcgct
     8461 ggccgcggtg ggcatcctgg cgtccatcgc gcggcggggc cgggcagaga ggggctgagg
     8521 gatgcgggtg gtcctggcag ccggggggac ggccgggcac atcttccccg ggctcgccct
     8581 ggcgcgtcga ctgcgggacg gccacggtgc gcaggtgctc ttcgtgggac gggagggagg
     8641 ccaggaggcg gtcctcgtcc ccgaggcagg cttccggctg gccacggtgg agtctcgacc
     8701 gttcgtgcgg aggctctcgc ctgccgctct cgctgcgccg gtggccgcgc tccgcgcctc
     8761 gcggcgggct cgagggttcc tccggaccgc cgacgtcgcc gtggggatgg gcgggtacac
     8821 gtcggttccg gtcgcgctgg ccgcctggcg ggagcgcgtg cccctcgtcc tccacgagca
     8881 gaacgcggtg cccggcctgg cgaaccgcgt ggccgcccgg tgggcgagag ccgtcgcggt
     8941 atcgttctcc gaggccgcgt ctcggttccc gcgcggggcc cgcacggtcg tcacggggaa
     9001 cccggtccgc caatcgatag cccggatcca ccaggagcgc gacgcgctgg cgaaggaggc
     9061 ccgcgaggag ctcggcctgg aggagagccg ccggaccgtc gtcgtcttcg gagggagcca
     9121 gggggccctc cacctggatc gggcggccgc cgacgccgcg cgactgctga gcggctccga
     9181 cctccagctc ctgatcgtca ccgggccggc ccacctcgag gaggtggagg gacggctccc
     9241 cggagggccg ctgccggtac gcgccgtccc gttcgtggac cgcatggagc tcgtgtacgc
     9301 cgtggccgac ctggccgtgg cccgtgcggg ggcgggcacc gtcgcagagc tgagcgtcgc
     9361 cggcgtgccc gccctgctgg tcccgtatcc ctatgccacc ggcaaccacc aggaggcgaa
     9421 cgcccgggcg atgcagcggg cggggggagc ggcggtcctg ctcgacgacc agctgaccgc
     9481 cgaggtgctc gcggagcggg tgatcgagat gctggagccg acccggctcg ccgcgatggc
     9541 cgagcgcgcc gcatcgttcg ggaggcccgg cgcggcggag gcgctggccc gggtcgtcgt
     9601 ggaggccgcc cgatgaagga ctacgagccc cggccgggga cgatccccac gctggagatc
     9661 ccccggctcg acctgcgtcg ggtccacctc gtgggcgtgg gcggcgcggg gatgagtggg
     9721 atcgccaggc tgctgctcgc tcgggggatc gccgtgtcgg gttccgacct caaggactcc
     9781 cccgggctcg cgctcctgcg cggcgccggg gcggaggtga ccgtggggca tcgccccgag
     9841 aacgtcggct cggccgaggc ggtggtggtc tcgacggcga tcggccggga caaccctgag
     9901 gtcgtggcgg cccgccgacg ggggctcccc gtctacgctc gggcccaggt gctggccgcc
     9961 atgatggcgg agcggcgggg catcgccatc gccgggaccc acgggaagac gaccacgacc
    10021 tcgatgacgg ccgtgatctt cgagcgcgcc gggctcgacc ccacgtacgt cgtgggcgga
    10081 gacctgaacg agagcgggag caacgcgcgc tcgggggatg gtgactactt catcgccgag
    10141 gccgacgagt ccgacggctc cttcctcctg ctcgatcccg agatcggcgt ggtgacgaat
    10201 gtggagccgg accacctcga cttctaccgg ggcggggagg acgtcgaggc ggcgttcgcg
    10261 gccttctgcc gtcgggccga tcggctcgtc gcctgcgggg acgatccggg ggtccggcgc
    10321 gtcctggacg ccgcgccgag cgaggtcgtc acctatggca tgggcgaggg caacgacgtc
    10381 cgcctgggga gcgtcgagac catgaacggc gaagggcgag gcgtcgtcgt tgtcgacggg
    10441 gagcgcgtcg aggtgtcgct ctccatcgtc ggccgccacc acctcctgaa cgcgaccgcg
    10501 gcgatgctga ccgcccgagc cgtgggcgtg ccactcgaat ccgcggccga ggcgctccgg
    10561 agcttcacgg gggtgcggcg ccggttcgag ccgcggggcg acgcgcgcgg ggcacggttc
    10621 gtggacgact acgcccacca tcccaccgag atcacggcga cgctcgaggc ggctaggtcc
    10681 acgggaccgg cccggctcgt ggccgtcttc cagccccacc gctacacgag gaccgaggcg
    10741 ctctggcggc cgctcggcgg cagcctggcc accgcggacc tcgtcgtcct caccgacgtg
    10801 tacggcgcgg gtgagatgcc cgtcccgggg gtgaccggga agctcctggt cgactcgctg
    10861 ctggaccggg cgccccgggc ccgggtgatc tacctgccgt cgcgagccga cgtggcgccg
    10921 ttcctggccg gcgagatccg taacggcgac ctcgtcctga cgctgggagc cggcgacgtc
    10981 acgatggtgg cggacgagac gctctccagg atccgggagg cgaacggtgg cggctgacgc
    11041 cgggcgcgac gcggcccgga tcctgaccga ggtggctgga gaccgggtcc gccgggactt
    11101 cccgctggcc cctctgacgt ccttccggct cggcggccct gccctcgtgt acctggaggc
    11161 gtggtcgcag gaggacctgg cggcgctggg gcgagccctg gaggaaacgg ggctccccct
    11221 gctcgtgatc gggaagggat cgaacctgct gatctccgac gaggggttcc cggggatcgt
    11281 ggtgcgactg ggcaagggct tccgctgggc ggggagggag gagctgcgga tcacggccgg
    11341 aggagcgatg cctctccccg cggtggcgaa cgtcgccctg gcgcactcgc tggcggggct
    11401 ggagttcggc gtggcgatcc ccgcgtcgct ggggggctct gtccgcatga acgcgggggc
    11461 ccaccaccgg tcgctcgacg aggtcctcgc gtcggtcgag atctacgacg cgcgcgctcg
    11521 ggagcctcgc atcatcccgg cgatcgagat ggggttctcc tatcgggatt cgcgtctccc
    11581 ggagttgagc atcgtgatcg cggcgaccgc cgagctccgg gcggacgacc cggccgcgat
    11641 ccgtgcacgg atggacgagg cccggaggtg gcggcgcgag acccagcccc tcgccgagcc
    11701 gaactgcggg agcgtcttca agaaccccga gggtgaccac gcggcgcgcc tcatcgaccg
    11761 ggcgggggcc aagggccttc ggctcggcgg ggcgcgcgtg tcggagaagc acgcgaactt
    11821 cttcatcgcc ggcgaagggg cgacggcgtc ggacgtccgc gccctgatcg aggaggtgcg
    11881 gcgacgggtg cacgacgcgg cgggtgtctg gctcgagccc gaggtccatc tcgccggatc
    11941 gttcgacgag gagggagctg cgtgacgcgg agacggaatc gggccgggat cgtggtcctc
    12001 gccgcggtgc tgctggggat cctggtcgga acggtcgccc tccgctcgcc gctcttcgag
    12061 gttcgccggg tggaggtgcg gggggccggg cggctcgccc cctcggaggt acgggtcctc
    12121 gtggagggcg ctcgcggccg caacctgtgg ctggtcccgc tcgatgagat cgctcggtcc
    12181 gtgtccgctc acccctggat cgcgcgggcg gaggtgggtc gtcgcctccc ctcgacgctg
    12241 gtggtggagg tcgaggagcg catc
//
LOCUS       DASUTW010000283         7981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1390050, whole genome shotgun sequence.
ACCESSION   DASUTW010000283 DASUTW010000000
VERSION     DASUTW010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7981
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390050"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..613
                     /locus_tag="VF058_09590"
     CDS             98..613
                     /locus_tag="VF058_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020525939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX6208592.1"
                     /translation="MGEAREFGRYFEDFEVGDVYKHRPGKTITEYDDHLFCMITMNHH
                     PLHTNVHYAEEATQFGRNVVVGNLVYSLALGMSVPDVSGKAIANLEVESLKHAAPMFH
                     GDTLYAETTVLDKKESRSKPDRGVVSVETRGYNQDGQEVLYFRRKVLIPKRPAEEGHP
                     WPGWDIKRPKE"
     gene            complement(617..1726)
                     /locus_tag="VF058_09595"
     CDS             complement(617..1726)
                     /locus_tag="VF058_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6208593.1"
                     /translation="MTTETTTVRVLLVDDVSASRETLRRALAFDERIEVVGEAGSGPE
                     AVEKAEQLRPDLVLMDVRMPDGDGVEATRHIVTRFPQIRVVALTAHDDVDTVRDMLTA
                     GATGYALKGASVDELLGAVHKAWRGEAHIDERVLPSAVHELRRLLREERERRTEVERL
                     ARIRQEFVQVLSHELRTPLTVMSGALRYLIRRGLAAEEEPLIASALQRGIELERMIEG
                     LELIGESRHDDAASATPCGAIEAALVRVEGDPDAVECEGGVWPGVRDRHLARIAFELI
                     DNAMRHGRPPVEVRAWASDGLAHLVVSDGGDADLRPELFEAFSAGDMSATRSRGGLGL
                     GLFVAQRLCEADLGKLAIRRDRGRTIAEVVYEIPA"
     gene            1867..2556
                     /locus_tag="VF058_09600"
     CDS             1867..2556
                     /locus_tag="VF058_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX6208594.1"
                     /translation="MRDLDALRGEIVSCTRCPRLVEWRERAARERPKRYEGWEYWARP
                     LPGFGDPRARLLIVGLAPAAHGGNRTGRIFTGDRSGDWLFRSLHRAGFANQPESVSRD
                     DGLRLDGCYVAAVNRCAPPGNRPSPEERDTCLPYLVRELELLTGVRVIVALGSFAWDG
                     ALRALRARGVEIPRPKPRFGHGVEVEIGPYRLLGSYHPSQQNTFTGKLTEEMFDAVFE
                     RARELIEGGAR"
     gene            2553..3125
                     /locus_tag="VF058_09605"
     CDS             2553..3125
                     /locus_tag="VF058_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208595.1"
                     /translation="MTRDPGWREAMRRLPTLLIPLGREIGISHLRTRVPAIIYVRAVF
                     LSFVFLPYVFGAFLLLLGFPLGGAPFEPIEVGLAAAGLGGAVAAARTRSRELRADDAT
                     QAADRYVSLFFLAAALASAGAMFGFVGVFITEELWVYLPGLIGSTLALVWVAPTIRDV
                     ERRQRQISLGGSMVSLVDALLDPLPPRTKP"
     gene            3147..5099
                     /locus_tag="VF058_09610"
     CDS             3147..5099
                     /locus_tag="VF058_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6208596.1"
                     /translation="MPRGHFPMVLREEDKRPIERATVRRVVQTFRPYRRRVLLIALTI
                     LVTAGLGVVNPLLIKVIFDTALFTPDGVQLGALYFWVSLMVAVPIVAGVISIGQTYLS
                     NIVGQRVMQDLRNALYRHLQRMPLRFFTSTRTGEIQSRLSNDIGGVQSVVTDTASSLL
                     SNTVIVLSTVIAMAILSWQLTVLSLAMLPFFLWLTAKVGKARQQVAASTQRSLADLSA
                     LAEETLSVSGVLLSKAFGRQQYHEDRFSKENERLAGLQIRQTMIGRSFFATVQIFFSI
                     APALVYLVAGWVISGSPSPAAAGITAGTIVAFTTLQSRLFFPIGQMLQVSVEVQSAMA
                     LFDRIFEYLDMEPEIQDRPDAVRLVHDDVEGRVRFRDVWFRYEGAALEPEPDGEPEQD
                     GERATPRRLWTLQDVDLEIEPGQLAALVGPSGAGKTTITYLVPRFYDVQRGAVEIDGR
                     DVRDVSLESLGETVGMVTQETYLFHASVRENLMYGRPDATEEEMIAAAKAAFIHDRVM
                     LLPEGYDTPVGERGYKMSGGEKQRLAIARVILKDPKILILDEATSSLDTTSERLVQQA
                     LRPLMEGRTTIAIAHRLSTILGADVIFVLDEGRVVERGTHDELLERGGLYATLYERQF
                     HGGSVEAELSDGVLLPSGEFVPRESA"
     gene            5124..5759
                     /gene="pgl"
                     /locus_tag="VF058_09615"
     CDS             5124..5759
                     /gene="pgl"
                     /locus_tag="VF058_09615"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX6208597.1"
                     /translation="MERRLLVVDDLVSAALGVFLGLGPRTIALAGGATPQPVYAALAE
                     HVYPWEEVDAFMGDERCVPLDHPESNFGKAREALLSKVPARAHPMPGETCDAEAYERT
                     LRERFGDEVPSLDLVFLGIGEDGHTASLFPGDPALDERQRWVVPVERPDFRRLTLTFP
                     VLNAAAAVMFLIEGEPKREALTRLLDGDPEVPAARVEAERVLVVADRAAAG"
     gene            complement(5760..6533)
                     /locus_tag="VF058_09620"
     CDS             complement(5760..6533)
                     /locus_tag="VF058_09620"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX6208598.1"
                     /translation="MINRKQRFRHRPDPIMPHEGPLPAEGEIASLVGGKSGALRAAIF
                     GVNDGLVSNLSLIMGVAGAGVSPEVILLAGIAGLLAGAFSMAAGEYISMRVQRELFER
                     LIHLEAHELGVDPEGETEELAELLAKRGIPEELAPRVAESLMEDPATALDVHAREELG
                     LDPEELGSPWGAAASSFVTFALGALVPLAPYLFAEGALAAWLAVGLALGALFGVGALM
                     TMFTGRSAALSGGRMMLIGGAAALVTFFVGKLLDVTALG"
     gene            complement(6530..7387)
                     /locus_tag="VF058_09625"
     CDS             complement(6530..7387)
                     /locus_tag="VF058_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX6208599.1"
                     /translation="MDHVVFEREPDLDRPVLICAFTGWNDGGEAASTAAAYLRDRWSA
                     ERFARLDPEEFFDFQVTRPTVRLVDGVTRRIEWPENDFYAASLPHRDVALFLGIEPNV
                     RWRTYTEQIIGTARAIGAESILLLGAFLADVPHTAPSPVTGSAPDEETAKRLGLAGSR
                     YEGPSGIVGVLHDAATRAGLETISFWAAVPHYLPAGPNPRAALALADKVARFLDVEIA
                     TDTLERAGATWESQVTAMIEDNEELASYVEQLERSAQESDDLGEVPSGEALAAELERF
                     LRDQRGDGS"
     gene            complement(7413..>7981)
                     /locus_tag="VF058_09630"
     CDS             complement(7413..>7981)
                     /locus_tag="VF058_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX6208600.1"
                     /translation="LRGIRLDSGDLGELAHRARAMLDEAGLSEVRIFASGGLDERKVA
                     ELANTPIDAFGVGSKVGVAEDSPTLDSVYKLVEYDGRPVAKLSTAKATLPGRKQVFRT
                     EGGDTLALRDESLPGEPVLVPAMRSGERLNRDTWREARDRCRADLAALPEDRHGLDAP
                     PIEIRRSEALAALDEEIRAGIRRRELSQ"
BASE COUNT         1105 a         2834 c         2845 g         1197 t
ORIGIN      
        1 ccttcctctc ctgggaacgt atgcccgcgg gcggcgcggt caaccgggcg ccgaccgtcg
       61 gtccgctagc atgccagcga gaccaaggag ggacgcgatg ggcgaggcac gagagttcgg
      121 gcggtacttc gaggacttcg aggtcggcga cgtctacaag caccggcccg gcaagacgat
      181 caccgagtac gacgaccacc tcttctgcat gatcacgatg aaccaccacc ccctccacac
      241 gaacgtgcac tacgccgagg aggccaccca gttcgggcgc aacgtcgtag tggggaacct
      301 cgtgtactcg ctcgcgctcg ggatgagcgt ccccgacgtc agcggcaagg ccatcgccaa
      361 cctcgaggtc gagtcactca agcacgcggc gccgatgttc cacggcgaca ccctctacgc
      421 cgagaccacc gtcctcgaca agaaggagtc gaggtcgaag cccgaccgcg gcgtcgtctc
      481 cgtggagacg cgcggctaca accaggacgg gcaggaggtg ctgtacttcc gccgaaaggt
      541 cctgatcccg aagcggcccg ccgaggaagg gcacccctgg cccggctggg acatcaagcg
      601 gccgaaggag taggcctcag gcggggatct cgtagacgac ctcggcgatg gtccgcccgc
      661 ggtcgcggcg gatcgcgagc ttccccaggt cggcctcgca cagccgctga gccacgaaca
      721 ggccgagccc gaggcctccc cggctcctcg tggcggacat gtcgccggcc gagaacgcct
      781 cgaacagctc gggcctgagg tcggcgtccc cgccgtcgga gacgaccagg tgcgccagcc
      841 cgtcgctggc ccacgctcgc acctcgaccg gaggccggcc gtgccgcatc gcgttgtcga
      901 tcagctcgaa ggcgatcctc gccaggtgcc ggtcgcggac ccccggccaa accccgcctt
      961 cgcattcgac ggcgtcgggg tcgccctcga cccgcaccag cgccgcctcg atcgcgccgc
     1021 acggggtggc cgaggcggcg tcgtcgtggc ggctctcgcc gatcagctcc aggccctcga
     1081 tcatccgctc gagctcgatg ccccgctgga gggcggacgc gataagcggc tcctcctcag
     1141 cggccaggcc gcggcggatc aggtagcgga gggcgccgga catcaccgtc agcggcgtgc
     1201 gcagctcgtg ggagaggacc tggacgaact cctgacggat gcgcgccagc cgctcgacct
     1261 cggtgcgccg ctcgcgctcc tcgcgcagga gccgccgcag ctcgtgcacg gcgctcggca
     1321 gcacccgctc gtcgatgtgg gcctccccgc gccaggcctt gtggacggcg cccaggagct
     1381 cgtccaccga ggcgcccttc agcgcgtagc cggtggcgcc ggccgtgagc atgtcgcgga
     1441 ccgtgtccac gtcgtcgtgc gcggtgagcg ccacgacgcg gatctgcggg aaccgcgtca
     1501 cgatgtgacg cgtggcctcg accccgtcac cgtcgggcat ccgaacgtcc ataagcacga
     1561 ggtcgggccg gagctgctcg gccttctcca cggcttcggg gccggagccc gcttcgccca
     1621 cgacctcgat ccgctcgtcg aaggcgagcg cacgtcgcag cgtctcccgg ctggcggaga
     1681 cgtcgtcgac gaggaggacc ctgacggtcg tggtctctgt ggtcatcgag ttgatacctc
     1741 tggcatcggc ccggtgcggg agcgcttgag ccatcatggc tggacggcct cggcatttcg
     1801 accgcccgtt ggggggattc cgtcctcccc aagcggccca ccacacctgc ttcaatcgga
     1861 cccctcatgc gggacctgga cgcgctccgc ggcgagatcg tctcgtgcac gcgctgcccg
     1921 aggctcgtcg agtggcgcga gcgggccgcc agggagcgcc cgaagcgcta cgagggctgg
     1981 gagtactggg cccggccgct gcccgggttc ggggacccgc gggcccggct gctgatcgtc
     2041 ggcctcgcgc ccgcggccca cggcgggaac cggacgggcc gtatcttcac gggggatcgg
     2101 tcaggggact ggctgttccg ctcgctgcat cgagcgggat tcgccaacca gcccgagtcg
     2161 gtgtcgcgcg acgacgggct gcggctggac ggctgctatg tcgcggcggt gaacaggtgc
     2221 gccccgcccg ggaaccgccc cagcccggag gagcgcgaca cctgtctgcc gtacctcgtg
     2281 cgcgagctgg agctgctgac gggggtccgt gtgatcgtcg cgctggggtc gttcgcctgg
     2341 gacggcgcgc tgcgcgcgct gcgggcccga ggggtggaga tcccgaggcc gaagccgagg
     2401 ttcggccacg gcgtcgaggt cgagatcggc ccgtatcgct tgctcgggtc gtaccatccg
     2461 agccagcaga acaccttcac ggggaagctg accgaggaga tgttcgacgc cgtcttcgaa
     2521 cgagccagag agctcatcga aggaggggcg aggtgacgcg agatcccggc tggcgggagg
     2581 cgatgcgccg gctcccgacg ctgttgatcc cgctcggtcg agagatcggc atttcccacc
     2641 tgcggacgcg ggtgccggcc atcatctacg tccgcgccgt gttcctctcg ttcgtgttcc
     2701 tcccgtacgt cttcggggcc ttcctgctcc tgctgggctt ccccctgggc ggcgccccct
     2761 tcgagcccat cgaggtcggc ctcgcggccg cgggtctggg cggggccgtg gccgcggcgc
     2821 ggacgcggtc gagggagctc cgggccgacg atgcgaccca ggctgcggat cggtacgtgt
     2881 cgctcttctt cctcgccgcc gccctcgcct cggcgggcgc gatgttcggg ttcgtggggg
     2941 tcttcatcac cgaggagctc tgggtgtacc tgcccgggct gatcgggtcc acgctggcgc
     3001 tcgtctgggt ggccccgacg atccgggacg tcgagcggcg gcagcggcag atcagccttg
     3061 gcggctccat ggtctccttg gtggacgccc tcctcgatcc actcccgccg cgaaccaagc
     3121 cctgacggcg gctatcctta gatcccgtgc ctcgaggtca cttcccgatg gtcctccggg
     3181 aggaggacaa gcgtccgatc gagcgggcca ccgtccgcag ggtcgtccag accttccgtc
     3241 cctaccgacg gagggtgctc ctcatcgcgc tcacgatcct tgtcactgcc ggcctgggcg
     3301 tcgtcaaccc gctcctgatc aaggtcatct tcgacacggc cctgttcacg cccgacgggg
     3361 tgcagctcgg ggcgctgtac ttctgggtgt cgctgatggt ggccgtcccc atcgtggccg
     3421 gcgtgatctc gatcgggcag acctacctct ccaacatcgt gggacagcgc gtgatgcagg
     3481 acctgcggaa cgcgctctac cggcacctcc agcggatgcc gctccgcttc ttcacctcga
     3541 cccggaccgg tgagatccag tcccggctct cgaacgacat cggcggcgtg cagagcgtgg
     3601 tgaccgacac ggcctcgagc ctcctgtcga acacggtcat cgtcctctcc accgtgatcg
     3661 cgatggcgat cctctcctgg cagctcaccg tgctctccct ggcgatgctg cccttcttcc
     3721 tctggctgac ggcgaaggtg gggaaggccc gccagcaggt cgcggcctcg acccagcggt
     3781 cgctggccga cctctcggcg ctggccgagg agaccctctc ggtctccggg gtcctgctct
     3841 cgaaggcctt cgggcgtcag cagtaccacg aggaccggtt ctcgaaggag aacgagaggc
     3901 tggccgggct ccagatccgc cagaccatga tcgggcggtc gttcttcgcc acggtgcaga
     3961 tcttcttctc gatcgcgccg gcgctcgtct atctcgtggc cggctgggtg atcagcggca
     4021 gcccgagtcc ggcggccgcg gggatcacgg ctgggacgat cgtggccttc accacgctgc
     4081 agtcaaggct gttcttcccg atcgggcaga tgctccaggt cagcgtcgag gtccagagcg
     4141 cgatggcgct gttcgatcgg atcttcgagt acctcgacat ggagcccgag atccaggacc
     4201 ggccggacgc ggttcggctg gtgcacgacg acgtggaggg gcgggtgcgg ttccgggacg
     4261 tctggttccg gtacgagggc gcggcccttg agcccgagcc cgacggcgag cccgagcagg
     4321 acggcgagcg ggcgacgccg cgccgactgt ggaccctcca ggacgtggac ctcgagatcg
     4381 agccgggcca gctggcggcg ctggtcgggc cgagcggcgc cggcaagacc acgatcacgt
     4441 acctggtccc ccgcttctac gacgtccagc ggggtgcggt ggagatcgac ggccgagacg
     4501 tgcgggacgt ctcgctggag tcgctgggcg agacggtcgg gatggtgacc caggagacct
     4561 acctcttcca cgccagcgtc cgcgagaacc tgatgtacgg gcggcccgac gccaccgagg
     4621 aggagatgat cgccgcggcc aaggccgcgt tcatccacga ccgggtgatg ctgctgcccg
     4681 agggctacga caccccggtg ggggagcgcg gctacaagat gtcgggcggc gagaagcaac
     4741 ggctggcgat cgcccgggtg atcctgaagg acccgaagat cctcatcctc gacgaggcga
     4801 cctcctcgct cgacacgacc tcggagcgcc tcgtccagca ggcccttcgc cccctgatgg
     4861 agggccgaac gacgatcgcg atcgcccatc gcctctctac gatcctcggg gccgacgtga
     4921 tcttcgtcct cgacgaaggg cgggtggtcg agcgcggcac gcacgacgag ctgctcgagc
     4981 gcggcgggct gtacgccacg ctctacgagc ggcagttcca cgggggttcg gtggaggccg
     5041 agctctcgga cggcgtcctg ctcccctcgg gcgagttcgt cccccgcgag tcggcctgag
     5101 ccctcccggg taggcttccg cccgtggagc ggaggctgct cgtcgtcgac gacctcgtca
     5161 gcgcggcgct cggggtgttc ctcgggttgg ggccacggac gatcgcactg gccggcggcg
     5221 ccaccccgca gccggtgtac gcggcgctgg ccgagcacgt gtatccctgg gaggaggtcg
     5281 acgcgttcat gggcgacgag cgatgcgtgc cgctcgatca tcccgagtcg aacttcggga
     5341 aggcgcgcga ggcgctgctc tcgaaggtcc cggcgcgagc ccaccccatg cccggcgaga
     5401 cgtgcgacgc ggaggcctac gagcggacgc tccgggagcg cttcggggac gaggttccct
     5461 cgctcgacct cgtcttcctc gggatcggcg aggacgggca caccgcctcg ctcttccccg
     5521 gcgatcccgc gctcgacgaa cggcagcggt gggtggtgcc cgtggagcgt cccgacttcc
     5581 gacgcctcac gctcaccttc ccggtactga acgcggcggc ggccgtgatg ttcctgatcg
     5641 agggcgagcc gaagcgggag gccctgacga ggctgctgga cggcgatccc gaggttcccg
     5701 cggctcgggt cgaggccgag cgggtcctcg tggtggcaga ccgcgcggcg gccggctgat
     5761 caacccaggg cggtgacgtc gaggagcttc ccgacgaaga acgtcacgag cgcggcggcg
     5821 cctccgatca gcatcatccg gccgcccgag agcgcggcgc tgcgtcccgt gaacatcgtc
     5881 atcagcgcgc ccaccccgaa cagcgccccc agcgccaggc ccaccgcgag ccacgccgcg
     5941 agcgcgccct cggcgaagag gtagggcgcc agcgggacga gggcgccgag cgcgaacgtc
     6001 acgaacgagg acgcggcggc gccccacgga gagccgagct cctcgggatc gagccccagc
     6061 tcctcgcggg cgtggacgtc gagcgccgtg gccggatcct ccatcaggct ctcggcgacg
     6121 cggggcgcga gctcctcggg gatcccccgc ttcgccagca gctccgccag ctcctcggtc
     6181 tctccctcgg ggtccacccc gagctcgtgg gcctcgaggt ggatcaggcg ctcgaagagc
     6241 tcgcgctgga cccgcatcga gatgtactcg cccgccgcca tcgagaaggc tccggcgagc
     6301 agccccgcga tgccggcgag caggatcacc tccgggctca cgcccgcgcc ggccacgccc
     6361 atgatgagcg agaggttcga gacgaggccg tcgttgacgc cgaagatcgc cgcgcgcagg
     6421 gcgccgctct tcccgccgac gagcgaggcg atctccccct cggcgggcag gggcccctca
     6481 tgcggcatga tcgggtcggg tcggtgccgg aaccgctgct tccggttgat cacgagccgt
     6541 cccctcgctg atcacggagg aagcgctcga gctcggccgc cagcgcctcg ccgctgggca
     6601 cctccccgag atcgtcgctc tcctgggccg agcgctccag ctgctcgacg taggaggcca
     6661 gctcctcgtt gtcctcgatc atcgcggtca cctgcgactc ccacgtggct cctgcacgct
     6721 ccagggtgtc ggtcgcgatc tcgacgtcca ggaatcgggc caccttgtcc gccagggcga
     6781 gggcggctct ggggttgggt ccggcgggga ggtagtgggg gacggccgcc cagaaggaga
     6841 tcgtctcgag gccggcccgc gtggcggcgt cgtgcagcac gccgacgatg ccgctcggcc
     6901 cctcgtaccg ggagccggcc agcccgaggc gcttcgcggt ctcctcgtcg ggcgcggagc
     6961 cggtcacggg cgagggagcc gtgtgcggca cgtcggccag gaaggcgccc agcagcagga
     7021 tcgactcggc tccgatcgcc cgtgccgtcc cgatgatctg ctcggtgtag gtccgccagc
     7081 ggacgttcgg ttcgatcccg aggaagagcg ccacgtcccg gtgggggagc gaggcggcgt
     7141 agaagtcgtt ctcgggccac tcgatgcggc gggtgacccc gtccacgagc cggaccgtgg
     7201 ggcgggtcac ctggaagtcg aagaactcct cggggtcgag gcgggcgaag cgctcggcgc
     7261 tccaccggtc gcggaggtag gcggcggcgg tgctggccgc ctcgcctccg tcgttccatc
     7321 cggtgaacgc gcagatcagg acgggccggt ccaggtcggg ctcgcgctcg aagacgacgt
     7381 ggtccacgtt cacagtctcc cccgtcggcg gctcattggc tcagctcccg gcggcggatg
     7441 ccggcccgga tctcctcgtc gagcgcggcg agggcctcgg acctgcggat ctcgatgggc
     7501 ggggcgtcga gcccgtgacg gtcctcgggc agcgcggcga ggtcggcccg gcagcgatcg
     7561 cgcgcctccc gccaggtgtc tcggttcagc cgctccccgc tcctcatcgc ggggaccagc
     7621 accggctcgc cggggagcga ctcgtcccgc agggcgagcg tgtcgccgcc ctcggtgcgg
     7681 aagacctgct tgcggccggg cagtgtggcc ttcgcggtgg agagcttggc cacggggcgg
     7741 ccgtcgtact ccacgagctt gtagacgctg tcgagggtgg gggagtcctc ggccacgccc
     7801 accttgctcc ccaccccgaa cgcgtcgatg ggcgtgtttg ccagctcggc caccttccgc
     7861 tcgtccaggc caccgctggc gaagatgcgg acctcggaga ggccggcctc gtccagcatg
     7921 gcgcgggccc ggtgcgcgag ctccccgagg tccccgctgt cgaggcggat ccctcggagg
     7981 t
//
LOCUS       DASUTW010000284         5783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_354789, whole genome shotgun sequence.
ACCESSION   DASUTW010000284 DASUTW010000000
VERSION     DASUTW010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354789"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2569)
                     /gene="aceE"
                     /locus_tag="VF058_09635"
     CDS             complement(<1..2569)
                     /gene="aceE"
                     /locus_tag="VF058_09635"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX6208601.1"
                     /translation="MMEPFKYQLPDADPQETAEWLEALRQLMEDSPSRARFLIYQLTR
                     EAQARHLGVPSMVSTPYINTIPPEQEPEFPGDEDMERRIRRLIRWNAAVMVSRANEHY
                     EGIGGHISTYASGASLVEVGFNHFFRGRSHPGGGDHVYFQGHGAPGIYARAFLEGRLT
                     EDQLEAFRREAFGDGLSSYPHPRLMPRFWEFPTVSMGLSPIAAVYQARFDRYLLNRGI
                     RDTSDQRVWAFVGDGEMDEPESLAGLTIASREGLDNLIFVVNCNLQRLDGPVRGNGKV
                     IQELEALFRGAGWHVIKVIWGREWDDLLARDSDGVLVSKMNQTVDGEFQRYAVDDGAY
                     IREHFFGPDPRLQELVAHLSDEDLRRLRRGGHDYSKLYAAYKRATELTGAPVVILAKT
                     VKGWTIQGLEARNVTHQQKKLASQELKVFRDRLELPIEDDEIHEGEAPYMTAERFPEV
                     AEYLRERRSELGGFVPERRVAPQEMTLPGDDVYAEFDAGSEQPVATTMAMVRLIRKLM
                     RDERVGERVVPIIPDEARTFGMESLFPEFKIYAPHGQVYEPVDAAHVLAYKEAKDGQV
                     LEEGITEEGSMASFQAAGTSYATHGEPMIPFFIFYSMFGFQRIGDLIWQAADMRARGF
                     LLGATAGRTTLNGEGLQHEDGHSLLLASTNPAVLAYEPTFAFEIAAIVRDGLRRMQDE
                     GEDLIYYLTLQNEPYPQPKRPEGLEDDEILRGMYRFRSTDGERRLRAQILGSGSIMQE
                     ALRAQELLAGHHDVAADIWSATSYHAMRLEAIEAERWSRLHPGEAPRTPLVTRQLEGA
                     EGPVIAVSDWMRAVPDQIARWVPGPYRSLGTDGFGRSDTRPALRRFYEVDAEHVVVAT
                     "
     gene            complement(2628..3095)
                     /locus_tag="VF058_09640"
     CDS             complement(2628..3095)
                     /locus_tag="VF058_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX6208602.1"
                     /translation="MQRDREAGFTLVELMIVLLILAILIAIAIPTFLEYRRRASNRAV
                     QGHLNTALKVQRGIKADPPFTFSADGSELEAIEPGLDYDAAINPTCAAYVDQYCIRVE
                     MLGPDEVLLIALSTSGDYWAIRDVTDGPGAGTFYNQGGDAAPPAAAAVVDDSW"
     gene            complement(3155..3226)
                     /locus_tag="VF058_09645"
     tRNA            complement(3155..3226)
                     /locus_tag="VF058_09645"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3192..3194),aa:Val,seq:cac)
     gene            3435..4154
                     /locus_tag="VF058_09650"
     CDS             3435..4154
                     /locus_tag="VF058_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEX6208603.1"
                     /translation="MSDDIGRKMLAEFVGTFALIFVGAGSIVAASVIGGGDLAPNLVT
                     IALAHGLVIAVMVSAIGHVSGGHLNPAVTIGAWTAGKIRTGDAAAYLVTQLAAGVAGA
                     LLLRAVVPEDAWRSVSLGATLLGDVTVAQGIAIEAVLTFLLVWVIFAVAVDPDGTFRA
                     VAGLPIGLTVALDIMMGGPFTGGSVNPARSFGPAVAGGYWTDHWVYWIGPVAGGVLAA
                     VLYDLLVLRRRRPEPTLEEAP"
     gene            4159..5223
                     /locus_tag="VF058_09655"
     CDS             4159..5223
                     /locus_tag="VF058_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007029492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX6208604.1"
                     /translation="MSPPPEMLEALRLLVEAESFSSDPGGLERCGLVLRDLCREILGS
                     PPEAEGHVLRWQRPGDRPVLLLCHYDTVWPTDTLAHFPFEVRDGVARGPGAFDMKAGI
                     VQGLFAIRDSSGPVTMLLTHDEERGSAESQEAIEREALASRAVLVLEPSADGGAVKTA
                     RKGVAHWRIEVKGRAAHAGLEPEKGVNAAIEIARQLERISSFGDPAAGTTVTVTMVRA
                     GSAENVVPEEAWASVDARVWTREEGERLERAFASLEPLTEGAHLSVKGGMNRGPLGRE
                     ASGDLYERLRRLGYDLPAAEVGGGSDGNFTAALGVPTLDGLGAEGGGAHARDEHVLVG
                     AMPLRAEMVARLIDDLAADG"
     gene            5216..>5783
                     /locus_tag="VF058_09660"
     CDS             5216..>5783
                     /locus_tag="VF058_09660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6208605.1"
                     /translation="MAEVAIRDLAEVDDLRSALELFERIWGAEDRMFAVEVLRAVATH
                     GGAILGAFLDDRMVGAQVAFLGRDDGELLLHSHVTGVEPTLQHEGIGSALKWAQRDWA
                     LERGIELITWTFDPMISRNAYLNLRKLGASARRFRRDFYGRMDDDINVGERSDRLEIT
                     WRLRDPRVEGAAAGGPVARDAEGAAVLLD"
BASE COUNT          806 a         1985 c         2111 g          881 t
ORIGIN      
        1 gcgtggccac gacgacgtgc tccgcgtcca cctcgtagaa gcggcgcagc gccggcctgg
       61 tgtcggagcg tccgaacccg tcggtcccga gcgagcggta gggccccggc acccaccgcg
      121 cgatctggtc ggggacggcc ctcatccagt ccgacaccgc gatcacgggc ccctcggccc
      181 cctcgagctg ccgggtcacg agcggagtgc ggggcgcctc acccgggtgg agccggctcc
      241 agcgctcggc ctcgatcgcc tcgagccgca tcgcatggta ggaggtcgcg ctccagatgt
      301 cggcggccac gtcgtggtgc ccggccagca gctcctgggc ccgcagagcc tcctgcatga
      361 tcgagcccga ccccaggatc tgggcccgga ggcggcgctc cccgtcggtc gagcggaacc
      421 ggtacatccc cctgaggatc tcgtcgtcct ccaggccctc gggccgcttc ggctgggggt
      481 agggctcgtt ctggagcgtg aggtagtaga tcaggtcctc gccctcgtcc tgcatcctgc
      541 gcaggccgtc gcggacgatc gccgcgatct cgaaggcgaa ggtgggctca taggcgagca
      601 cggcggggtt cgtggaggcg agcagcagcg agtgcccgtc ctcgtgctgg agcccctccc
      661 cgttcagcgt ggtccggccg gccgtggcgc ccaggaggaa cccccgggcc cgcatatccg
      721 ccgcctgcca gatcaggtcg ccgatccgct ggaacccgaa catcgagtag aagatgaaga
      781 aggggatcat gggctcgccg tgcgtggcgt aggacgttcc ggcggcctgg aaggaggcca
      841 tcgagccctc ctcggtgatc ccttcctcca ggacctgccc gtccttcgcc tccttgtagg
      901 cgaggacgtg cgccgcgtcg accggctcgt agacctgccc gtgcggcgcg tagatcttga
      961 actccgggaa gagcgactcc atcccgaagg tccgggcctc gtccgggatg atcgggacca
     1021 cgcgctcccc cacccgttcg tccctcatca gcttccggat caggcggacc atggccatcg
     1081 tcgtggccac gggctgctcg gaacccgcgt cgaactccgc gtagacgtcg tccccgggga
     1141 gggtcatctc ctggggcgcc acgcgacgct ccgggacgaa gccaccgagc tcgctccggc
     1201 gctcccgcag gtactcggcc acctcgggga agcgctcggc cgtcatgtac ggcgcctctc
     1261 cctcgtggat ctcgtcgtcc tcgatgggga gctccagccg gtcgcgaaaa accttcagct
     1321 cctgggaggc gagcttcttc tgctggtggg tgacgttcct ggcctccagg ccctggatcg
     1381 tccatccctt caccgtcttg gccaggatca ccaccggcgc tcccgtgagc tcggtggcgc
     1441 gcttgtaggc ggcgtagagc ttcgagtagt cgtggccccc gcggcgcagc cgtcggagat
     1501 cctcgtcgga gaggtgcgcg acgagctcct ggagccgcgg gtccggcccg aagaagtgct
     1561 cccggatgta cgcgccgtcg tccaccgcgt accgctggaa ctcgccgtcc accgtctggt
     1621 tcatcttgga gaccagcaca ccgtcgctgt cgcgggccag caggtcgtcc cactcccggc
     1681 cccagatcac cttgatcacg tgccacccag cgcccctgaa gagcgcctcc agctcctgga
     1741 tcaccttccc gttgccgcgg accggcccgt cgagccgctg gaggttgcag ttgacgacga
     1801 agatcaggtt gtcgaggccc tcacgggacg cgatcgtcag accggccagc gactcgggct
     1861 cgtccatctc gccgtccccg acgaaggccc acacgcgctg gtccgacgtg tccctgatcc
     1921 cccggttcag caggtagcgg tcgaagcggg cctggtacac ggccgcgatc gggctgagtc
     1981 ccatcgagac cgtggggaac tcccagaacc ggggcatcag ccgcgggtgg gggtaagagg
     2041 acaggccgtc gccgaacgcc tcgcggcgga aggcctccag ctggtcctcg gtcaggcggc
     2101 cctccaggaa ggcccgggcg tagatccccg gcgccccgtg gccctggaag tacacgtggt
     2161 ctcccccgcc cgggtgactc ctgccccgga agaagtggtt gaagcccacc tcgaccaggg
     2221 aggccccgga ggcgtaggtc gagatgtgcc ccccgatgcc ctcgtagtgc tcgttggcgc
     2281 gggacaccat gaccgcggcg ttccagcgga tcaggcggcg gatgcgcctc tccatgtcct
     2341 cgtcgccggg gaactccggc tcctgctcgg gcgggatcgt gttgatgtag ggggtggaga
     2401 ccatggacgg gacgccgagg tggcgagcct gcgcctcccg cgtgagctga tagatcagga
     2461 agcgagcgcg agagggcgag tcctccatca gctgccggag ggcctccagc cactccgcgg
     2521 tctcctgggg atccgcgtcg ggaagctggt acttgaacgg ctccatcatc gattccccac
     2581 ccctttcccc atcgatcgta cccaacggat gggtgagacg gagcccgcta ccaggagtcg
     2641 tccacgacgg cggccgcggc aggcggcgct gcgtccccgc cctggttgta gaaggtgccc
     2701 gcgcccgggc cgtcggtcac gtcgcggatc gcccagtagt cgcccgacgt ggagagcgcg
     2761 atcagcagca cctcgtccgg cccgagcatc tcgacccgga tgcagtactg gtcaacgtag
     2821 gcggcgcagg tggggttgat cgccgcgtcg tagtcgagcc ccggctcgat cgcctccagc
     2881 tcgctgccgt cggcgctgaa ggtgaacgga gggtcggcct tgatgcctcg ctggaccttg
     2941 agcgcggtgt tgaggtggcc ctgcacggcc cggttcgagg cccgccgccg gtactcgagg
     3001 aacgtgggga tcgcgatcgc gatgaggatc gcgaggatga gcaggacgat catgagctcc
     3061 acgagcgtga agcccgcctc ccggtcccgt tgcatgcgca gccgccgcat ttcattgaag
     3121 tgtcggcacg gaagaggcgt ccttgagcgc agggtgggcg cggcaggatt tgaacctacg
     3181 acctctcgcg tgtgaggcga gcgctctccc actgagctac gcgccctggc cgctccgagg
     3241 atagcatcag caggcccccg gaaaggtgac ggcggggcgt gcccgcgtcg ccccgccgcc
     3301 ggtccggacc cgaaggcccg gacgatgcgc gtagtgtgcc acggaatcac ggaaaggtgc
     3361 aaaggaaccg cgggtgcttg cgccctcccc cctcggccgg tttgctaggt cgcaccggac
     3421 ccgaagggag ggacatgagc gacgacatcg gacggaagat gctcgcggag ttcgtgggga
     3481 cgttcgcgct gatcttcgtg ggggccggat cgatcgtggc ggcctccgtg atcgggggag
     3541 gcgacctcgc ccccaatctc gtcacgatcg cgctggccca cggcctcgtc atcgcggtga
     3601 tggtctctgc gatcgggcac gtgtcgggag ggcatctcaa tcctgcggtt accatcggag
     3661 catggacggc ggggaagatc aggaccgggg acgccgcggc ctacctcgtg acccagctgg
     3721 ctgcgggcgt ggccggcgcc ctcctgctgc gcgcggtcgt cccggaggac gcgtggcgct
     3781 cggtctccct gggggccacc ctgctgggag acgtcaccgt cgctcagggg atcgcgatcg
     3841 aggcggtcct gacgttcctg ctggtctggg tgatcttcgc ggtcgcggtg gaccccgacg
     3901 ggaccttccg ggccgtggcc gggctgccga tcgggctcac ggtggcgctc gacatcatga
     3961 tgggcgggcc cttcacgggg gggtccgtga acccggcgcg ctcgttcggc ccggcggtgg
     4021 ccggggggta ctggaccgac cactgggtgt actggatcgg gcctgtggcc ggcggcgtgc
     4081 tcgccgcggt gctgtacgac ctgctcgtgc tgcgacgccg gcgcccggag cccacgctgg
     4141 aggaggctcc ctgacgaggt gagccctccc cccgagatgc tcgaggccct gcggctcctc
     4201 gtcgaggccg agtccttctc gtcggatccg ggcgggctgg agcggtgcgg cctcgtcctg
     4261 cgggacctgt gccgggagat cctggggagc cccccggagg ccgaggggca cgtgctgcgc
     4321 tggcagcgac ccggggatcg gcccgtcctc cttctctgcc actacgacac ggtgtggccc
     4381 acggacaccc tggcgcactt cccgttcgag gtccgggacg gcgtggcccg ggggcccggg
     4441 gccttcgaca tgaaggcggg gatcgtgcag gggctcttcg ccatccggga cagcagcggg
     4501 ccggtgacga tgctgctcac tcacgacgag gagcgcggga gcgccgagtc ccaggaggcg
     4561 atcgagcggg aggcgctggc ctcgcgggcg gtgctcgtgc tggagccctc cgccgacgga
     4621 ggcgcggtga agacggcgcg caagggcgtg gcccactggc ggatcgaggt gaaggggcga
     4681 gccgcgcacg ccgggctcga gcccgagaag ggcgtgaacg cggcgatcga gatcgcccgc
     4741 cagctggagc ggatctcctc gttcggggat cccgcggccg ggaccacggt caccgtgacc
     4801 atggtccggg cggggtccgc ggagaacgtc gttcccgagg aggcctgggc cagcgtggac
     4861 gcccgcgtgt ggacgcgaga ggaaggagag cgcctggaga gagccttcgc atcgctcgag
     4921 ccgctgaccg agggcgccca cctctccgtc aagggtggga tgaaccgggg accccttggg
     4981 cgggaggcga gcggggacct gtacgagcgg ctccggcgtc tcgggtacga cctcccggcg
     5041 gccgaggtgg gcggtgggag cgacgggaac ttcacggcgg ccctcggcgt ccccaccctc
     5101 gacgggctcg gcgccgaggg tggcggggcg cacgcccgcg acgagcacgt cctcgtgggc
     5161 gcgatgccgt tgcgggccga gatggtggcc cgcttgatcg acgatctggc cgccgatggc
     5221 tgaggtcgcg atccgagacc tcgccgaggt cgacgacctc cgctccgccc tggagctctt
     5281 cgaacggatc tggggtgccg aggaccgcat gttcgcggtc gaggtgctcc gcgccgtggc
     5341 cacgcacggc ggcgcgatcc tcggggcgtt cctggacgac cggatggtgg gggcccaggt
     5401 cgcgttcctg ggccgggacg acggtgagct gctcctccac tcgcacgtca cgggcgtcga
     5461 gccgaccctc cagcacgagg ggatcggctc cgcgctgaag tgggcgcagc gcgactgggc
     5521 gctggagcgt gggatcgagc tcatcacctg gacgttcgac ccgatgatct cgaggaacgc
     5581 gtacctcaac ctccggaagc tgggcgcctc cgcccgccgg ttccggcgag acttctacgg
     5641 tcggatggac gacgacatca acgtaggcga gcgctcggac cggctcgaga tcacatggcg
     5701 cctccgggac cccagggtgg agggtgcggc cgcgggcggc cccgtggctc gcgacgccga
     5761 gggggccgcc gtcctgctcg acg
//
LOCUS       DASUTW010000285         9655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_484073, whole genome shotgun sequence.
ACCESSION   DASUTW010000285 DASUTW010000000
VERSION     DASUTW010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9655
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484073"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VF058_09665"
     CDS             <1..810
                     /locus_tag="VF058_09665"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6208606.1"
                     /translation="VLVVHGITQGADGGLSDLAEELVPDGCRVLVPSRFGYLGSAMPD
                     DATPEMQADAFAEFIGALGITDAVVVAGSAGSTSALHLCVRHPARVRALVLISANVPG
                     SHQSQGMPPRPLFKAIFGSDPILWAMITYLPGVMARLSQVLVVPEGVQLIPGDEEKLS
                     RAMRQVLPGKRRVTGEVFDAYVSNPDVNRIELSQVLAPTLVMHARDDPGPPYASAVEM
                     AHRIPDAQLVTVESGGHLMLGDHADAISEVARFILEHGSSHAEGPQGDEGR"
     gene            complement(897..1169)
                     /locus_tag="VF058_09670"
     CDS             complement(897..1169)
                     /locus_tag="VF058_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208607.1"
                     /translation="MDDEELLARAEEALTEIKVSHGLSDRHASVLAALRIRLKGSAGS
                     SLEEMLAAAGDLKPGLEEEMAKAEDEPKPSLDDLLSEPDAKPERPS"
     gene            1249..2157
                     /locus_tag="VF058_09675"
     CDS             1249..2157
                     /locus_tag="VF058_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208608.1"
                     /translation="MSLRRSGVVAFLFLGILMLAPGAVAQENGIDDDDVVILTGRLNV
                     AEGESVQNAVIFNGPAVIEGTVEEWLVVFNGRTEITGTVREDVIVFAGDVVLRSGSEV
                     QGDVITLEDPQIEEGATIGGSVDDLQTRFDLYDSTFIGRFAWWLAYTVSTLVLGLVLL
                     FLAPRLDLAAITSLRDRAGAAIGFGLMVFFLLPIVAGLLLVTVVGIPLGLFLLGGLAL
                     IYSIGYVVGGLALGRLAVREPTSRYVAFFLGWGALRLVALVPFLGGLAWFVGTVLGLG
                     TLWVAGRWAPGEVRPPTPAPAPPATA"
     gene            complement(2260..4185)
                     /locus_tag="VF058_09680"
     CDS             complement(2260..4185)
                     /locus_tag="VF058_09680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX6208609.1"
                     /translation="MVTIALTFSLVGALILSRRKDNGLGWLFAVNGPVVVVSGLVRVL
                     GYRGLVGDLGFETGRSATWAADMSDPLVVALMTVFLYLLFPEGRLRSHGERRTALVAA
                     VGVLLATMGAFLEPHLQEYPDVGTPFALAIPAAVSWTLVGTGYAVIIGTVVSSIVLLI
                     RRLRRARGRERDQLRLLVWATVVATILIVPPIFSPPGASWTRVMYLLGGLGFLLIPVS
                     VGVAILRHRLLDIDLVIRRTVVVAVLGAFITVVYVGMVVGVGAMIGRSGNVVLSAVAA
                     AVVALAFQPLRRWAQQLSNRVVYGERATPYEVLHEFSERVAGSYGTEDVLPRMAAILG
                     EGTGASRAQVWMLFGSELRPVAVWPSGVRPSDAAWAPGPELPPMPDVSLAVPVRDDEE
                     LLGALSVTKDASDPVTQTEERLVGDLAHQAGLVLRNVRLVEDLRASRTRLVAAQDEER
                     RKLERDIHDGAQQHLVALTVKAQLADSMVDRDAAKARQLLAEIKAETTEALEALRDLA
                     RGIYPPLLADKGLAAALEAQARKVTVPTEVNADGAGRYPEQVEATVYFCCLEALQNTA
                     KYADASRATVSISASEGALTFQVSDDGRGFDPASTPRGSGLQNMSDRLEAVGGALEIR
                     SAPGGGTTLTGRVPLHA"
     gene            4526..5362
                     /locus_tag="VF058_09685"
     CDS             4526..5362
                     /locus_tag="VF058_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX6208610.1"
                     /translation="MRFKRRYRSRNVRDRRFATPARPGISGLPIGAAGGGIGGILVLL
                     LILFLNGTLGGGGAEAPEAPPQGQDPEAELVSFVSFVFDDAQSTWTDIFEESGRNYRP
                     AEMVLFRDATDTACGFASSDVGPFYCPADERVYLDLGFFEELRDRFGAKGDFAQAYVI
                     SHEVAHHVQHLLGINERVQRLSQQNPSQRNELSIQQELQADCFAGVWGKSTRERGLLQ
                     EGDVQEGLGAAAAVGDDRIQREATGRVNPETWTHGSAEQRRTWFQRGFRTGDGEACDT
                     FG"
     gene            complement(6585..7616)
                     /locus_tag="VF058_09690"
                     /pseudo
     CDS             complement(6585..7616)
                     /locus_tag="VF058_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003936224.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            7795..8670
                     /locus_tag="VF058_09695"
     CDS             7795..8670
                     /locus_tag="VF058_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208611.1"
                     /translation="MGTHRWLGVGLITLGAGLIVSAILGPLVLGIVDYPLSESLTNQT
                     LGLDAVSLTLVAPVSVLAGALALRGHPAGPVLGFGPAAYGWYMLLQFVVGPEYAYYPG
                     ALPLHLGLFVLSGTIAVGSWRAIRSEELPPATRRADRLRGVALLAFSGFLLSRYVPLV
                     ADALRGEGLSAEALEGVTMFWSIFLLDLGVVLPATIAAAVGVIAGAPWGRKAMYALVG
                     WWAFVPASVAAMAIVMVGNDDPNASTGQAAVFVVVAVLVLAFAAVVYRGLFRADRVAS
                     APAGEASRRHLTRVG"
     gene            complement(8674..>9655)
                     /locus_tag="VF058_09700"
     CDS             complement(8674..>9655)
                     /locus_tag="VF058_09700"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX6208612.1"
                     /translation="PEALDRALRVRRTLLAIWSMRGAPHIVPASDLEVFTLGALPLDA
                     ASFRRSMGGWSDALENAGFDVHETLDEMVAAARDLLDGRTMNVNEFRDLLRERVPALA
                     RVRKPEFARDPMPEPLFRALGTAGAVCIVSGRGTSSELGRTDQWLGSSPDPASKEEAR
                     AELARRFLHAYGPSSPRRFAEWTQRSTEDAKQAFSLIEGELEEVSFDGRSWMLRRDAK
                     TLSAPSEPRGVRLLPVQDPYLQQRDRATLLADAAHRKRLWQPIRGPGGVLSAGELVAT
                     WRSTIKGRRLEVTVEPFGRLTSAVREEVTGEAELAAVIRGCETARVTFGS"
BASE COUNT         1374 a         3375 c         3428 g         1478 t
ORIGIN      
        1 gttctcgtcg tccacggcat cacgcaaggt gcggacggcg gtctcagcga cctcgccgag
       61 gagctcgtcc ccgacggatg ccgagtgctc gtcccgtccc ggttcggcta cctgggctct
      121 gcgatgccgg acgacgcgac cccggagatg caggccgatg cgttcgcgga gttcatcggc
      181 gcgctgggca tcaccgatgc ggttgtggtg gcgggctcgg cagggagcac gtccgccctc
      241 cacctctgcg tgcgccatcc ggcccgggtc cgtgcgctcg tcctcatctc cgccaacgtt
      301 cccggttccc accaatcgca agggatgccc ccgagaccgc tgttcaaagc gatcttcggg
      361 agcgatccga tcctgtgggc catgatcacc tacctgcccg gcgtcatggc acggctgagc
      421 caggtgctgg tggttccgga aggggtgcag ctgatcccgg gggacgagga gaagctctct
      481 cgagcgatgc ggcaggtcct acccgggaag cggcgggtca ccggcgaggt tttcgatgca
      541 tacgtgtcga acccggacgt caaccggatc gagctctcac aggtcttggc cccgacgctc
      601 gtcatgcacg cgcgcgacga ccccggtcct ccctatgcga gcgcggtaga gatggcgcac
      661 cgcatcccgg atgcgcagct cgtcacggtg gagagcggtg gtcatctcat gctgggtgac
      721 cacgccgacg cgatcagcga ggtcgctcgc ttcatcctcg agcacggttc gtcccacgcg
      781 gagggtcctc agggggatga aggtaggtga tccaccgagc gatgggagcc atgaggatcc
      841 tgctggactg gtacagcacc cggtgggccg tggtcggttc cgatgggcgc tgacactcag
      901 gatggccgtt cgggcttcgc gtcgggctcg gacaggagat cgtccaggga gggcttgggc
      961 tcgtcctccg ccttcgccat ctcctcctcg agcccgggct tgaggtcgcc cgccgctgca
     1021 agcatctcct ccagcgaact gccggcgctc cccttcaggc ggatccgaag ggcggccagg
     1081 accgaggcgt gacggtccga caggccgtgg ctgaccttga tctcggtgag agcctcctcg
     1141 gcccgggcca ggagctcctc gtcgtccatg ggcacagtct gccaccggcg tcgcagacgc
     1201 acatcggatg agcttgcacg tgcgcggtcg gagggtcaga gtgacggcat gagcctgcgc
     1261 cggtccggcg tcgtggcgtt cctcttcctc gggatcctga tgctcgcgcc cggggccgtc
     1321 gcccaggaga acggcatcga cgacgacgac gtggtgatcc tgaccggtcg tctcaacgtc
     1381 gccgagggcg agtcggtgca gaacgcggtc atcttcaacg gccctgcggt gatcgagggc
     1441 acggtcgaag agtggctcgt cgtcttcaac ggaaggaccg agatcaccgg gaccgtgcgc
     1501 gaggacgtca tcgtgttcgc gggggacgtc gtcctgcgct ccggctccga ggtgcagggc
     1561 gacgtcatca ccctggagga tccccagatc gaggagggcg ccacgatcgg gggcagcgtc
     1621 gacgacctcc agacccggtt cgacctctac gactcgacct tcatcgggcg cttcgcatgg
     1681 tggctcgcct acacggtgtc gacgctcgtg ctcggcctcg tcctgctctt cctcgccccg
     1741 aggctggacc tcgcggccat cacgtcgctc cgagaccgtg cgggcgcggc gatcgggttc
     1801 ggcctcatgg tgttcttcct gctccccatc gtggcggggc tgctgctcgt caccgtcgtc
     1861 gggatcccgt tgggactgtt cctcctcggc ggcctcgctc tgatctactc gatcggctac
     1921 gtagtcggag ggctcgccct ggggcggctc gcggtcaggg agcccacctc ccgctacgtc
     1981 gccttcttcc tgggttgggg ggccctgcgg ctggtggcgc tggtcccgtt cctgggcggc
     2041 ctcgcttggt tcgtgggaac ggtgctcggg ctcgggacgc tctgggtcgc cgggcgctgg
     2101 gcgccgggcg aggttcggcc ccccacgccc gcaccggccc cgcccgccac ggcctgaacg
     2161 cgcggccccc cgacgctaag ggaacccgcc ggatcgcgat cggccgctgc ccaacgacac
     2221 cgtgacggag aggaacatcg agaggcgtcc aggctcgcat caggcgtgga gcggcacgcg
     2281 cccggtgagc gtcgtgccgc ctcccggcgc cgaccgtatc tcgagggctc cgcccacggc
     2341 ctccaagcga tcgctcatgt tctggaggcc cgagcctcga ggcgtggagg cgggatcgaa
     2401 cccgcgcccg tcgtccgaaa cctggaacgt cagcgcgccc tccgaggccg agatcgagac
     2461 ggtcgcccgc gaggcgtccg cgtacttcgc ggtgttctgc agcgcctcga ggcagcagaa
     2521 gtagacggtg gcctccacct gctccgggta gcgcccggcg ccgtcggcgt tcacctcggt
     2581 cggcacggtg accttccgtg cctgcgcctc gagggctgcg gccagcccct tgtcggccag
     2641 gaggggaggg tagatccccc gggccagatc gcgcagcgcc tcgagggctt cggtggtctc
     2701 ggccttgatc tcggcgagca gctggcgggc cttcgcagcg tctcggtcca ccatcgagtc
     2761 ggccagctga gccttcacgg tgagcgcgac gaggtgctgc tgcgcgccgt cgtggatgtc
     2821 ccgctccagc ttgcgacgct cctcgtcctg cgccgctacc agcctggtcc gcgaagcccg
     2881 caggtcttcg accagccgca cgttccggag caccaggccc gcctggtggg cgaggtcgcc
     2941 gacgagccgc tcctcggtct gcgtgaccgg atccgacgcg tccttcgtca cgctcagcgc
     3001 tccgaggagc tcctcgtcgt cccggaccgg gaccgccagg ctcacgtcgg gcatcggggg
     3061 cagctccggt ccgggtgccc aggccgcgtc ggacgggcga acgcctgatg gccacacggc
     3121 cacgggcctg agctcgcttc cgaagagcat ccaaacctgg gctcgcgacg cgcccgtccc
     3181 ctcacccagg atcgcggcca tcctcggcag cacgtcctcg gtgccgtagg atcccgccac
     3241 gcgctcggag aactcgtgca ggacctcgta cggggtggcc cgctccccgt agacgacgcg
     3301 gttggacaac tgctgagccc agcggcgcag cggctggaac gccagcgcaa cgaccgcggc
     3361 cgccaccgca gacagcacga cgttgccgga cctgccgatc atcgctccca cgccgaccac
     3421 catcccgacg tacaccacgg tgatgaacgc gccgaggacg gcgaccacga cggtccgacg
     3481 gatgacgagg tcgatgtcga gcagacgatg acgcaggatc gccaccccga ccgagacggg
     3541 gatcaaaagg aacccgaggc ctccgaggag gtacatcact cgggtccagg acgctccggg
     3601 cggcgagaag atcggcggaa cgatcaggat ggtggccacg accgtcgccc agaccaggag
     3661 gcggagctgg tctcgctcgc ggcctcgcgc cctccggagc ctccggatga ggagcacgat
     3721 cgaggagacg accgtgccaa tgatcaccgc gtaaccggtc ccgacgagcg tccacgacac
     3781 ggcggcgggg atcgcgagcg cgaagggcgt gccgacgtcg gggtactcct ggaggtgcgg
     3841 ctccaggaac gcacccatcg tcgcgagcag aaccccgacg gccgccacga gcgccgtccg
     3901 ccgctcgccg tgggaccgca ggcgtccctc cgggaacagg aggtagagga agaccgtcat
     3961 gagggcgacc accaggggat ccgacatatc ggctgcccag gtcgcggagc ggccggtctc
     4021 gaacccgagg tctccgacga gacctcggta gccgagtacg cggacgagcc cggagaccac
     4081 gacgacgggc ccgttgaccg cgaagagcca ccccagtccg ttgtccttcc gtcgcgagag
     4141 gatcagggca ccgacgaggg agaacgtcag tgcgatagtg accaccaaga ctccgaacgg
     4201 tagcgactgt gggacgccga ccgcctgtga cgagaccggc gcgaccatta cgaggctgat
     4261 cgcggatacg cccgtcgcca accccgcgag ggaccaaccc gtgatcgtgg ccgtccgagc
     4321 cctcacgctt cctcctcgtc cgtcgggtca ttctctcttt ttggcttggt acggaggaag
     4381 ggtggaagca ccacggaccg aggggatgtg ctcactacct tccgccgtgc ggaccgattc
     4441 gccgggatcc gcgagccgag cggttgcgac tcgcgtctcg atgatggggg cgtgggagtg
     4501 cgagcgacgg gggagaatct tggccgtgag gttcaaacgc cgctaccgaa gccgcaacgt
     4561 ccgcgatcgc aggttcgcca ccccggcgcg cccgggcatc tcaggcctcc cgatcggcgc
     4621 cgccggcggg ggcatcgggg gcatcctcgt cctcctgctg atcctcttcc tcaacggcac
     4681 gctgggcggg ggaggggcgg aggcgccgga ggcgcctccc cagggtcagg atcccgaggc
     4741 tgagctcgtc tccttcgtct cgttcgtgtt cgacgacgcg cagagcacgt ggacggacat
     4801 cttcgaggag tcggggagga actatcggcc ggccgagatg gtgctgttcc gggacgccac
     4861 ggacacggcc tgcggcttcg ccagctccga cgtcggaccc ttctactgcc ccgccgacga
     4921 acgcgtctac ctggatctcg ggttcttcga ggagttgcgg gatcggttcg gagccaaggg
     4981 ggacttcgcc caggcctatg tgatctccca cgaggtcgcc catcacgtcc agcacctcct
     5041 cgggatcaac gagcgggtcc agcgtctctc ccagcagaac ccctctcagc ggaacgagct
     5101 gtcgatccag caggagcttc aggcggactg cttcgccggg gtatggggga agagcacgag
     5161 ggagcgaggg ctgctgcaag agggcgacgt ccaggaaggt ctgggagccg cggccgcggt
     5221 gggggacgac cgcatccagc gggaggcgac cggccgggtg aaccccgaga cgtggaccca
     5281 cggttccgcg gagcagcgcc gcacctggtt ccagcggggg ttccggaccg gggacggcga
     5341 agcctgcgat acgttcggct gagggtagca gcggtcccga agagcagggc gggatacgat
     5401 cccggcgtga cggaagagcg ggcttccagg gacgacgatc tcgtggacga ggcgagcagg
     5461 gagtccttcc cggccagcga tcctccgtcg ttctgggccg ggcccccgcg ggaaagccct
     5521 aagcccaagg atgcctccga cgaaccgcgg atcaccgcgg gccctgacgg ctgatccgca
     5581 gcagccactc gtcactcccg ggtatcgtgg ggcccgtcgt gggcgacgtc tcggtggtat
     5641 tcgggaccgg cccgctcggg ctggcggtga tccgccggct cgcgggtcga ggggagcggg
     5701 tgagggccgt caaccgctcc gggcgtgccc agacgcccga cggcgtggag gtcgaggccg
     5761 ccgacgtcgc cgatcgcggg gacgccgtcc gggcgtgtcg gggcgcgacc gtcgtctacc
     5821 actgcgcgaa ccctccctat catcgatggg cggagctcca cccgccgctc atggccggga
     5881 tcatcgaggg agtctccgcc agcggtgccc gcgtggtgtt cggcgacaac ctgtacgctt
     5941 acgggccggt cggcggtccc ctcaccgagg agcttccgta ccgcccgctc ggaccgaacg
     6001 ggaggacgcg agccgagatc gccgccgcgc tgatgcgggc ccacgacgag ggcagggtgc
     6061 gagcgacgat cgggcgtggc tcggacttct tcggtccgca cgcccgacgt tcgaccgttg
     6121 gggatcgggt gttcgagcgg gcgctggccg ggaagccggc tcagctcctg ggcgaccccg
     6181 aggcgcctcg ctcggtgacc tacatcgatg acttcgcccg ggcgctggtc gcgctggcgg
     6241 acagcgaaga ggccgggagc gtttggcacg ttccgtgccc gcccccgctg ccgacgcgac
     6301 gcttcgtcga gatggtcttc cagggagttg gccgtcggcc gaggatccgc tccgcccccc
     6361 ggtgggtgct gtcgctggcg tcccccttca atccgacgat ccgtgccgtc agggagcagc
     6421 tctaccagca cgagcacccg tggatcgtca gcagcgagaa gttcgagggg gcgttcggct
     6481 ggggggcgac gccgctcgcg gacgcgatcc cggccaccgt ggcctggtac cggggtcggc
     6541 gctgacgcgt ccgcgagcgg cgtcgcccgg ccgcagcctc tcggtaggac caccttgagg
     6601 gcgcccgtct cgcccgctct ggagaactcg tcgtacgcct tggggaagtc ccccaggtcg
     6661 tagcggtggg tgacgaagcg gcccacgtcg agctgcccgg acccgatcag gcgcagcagg
     6721 gtgggcgtcg agtacgtgtc caccaggccc gtggtgatcg tgatgccctt gatccacagg
     6781 tcctcgagat ggagggtggc cggcttcccg tgcacgccca cgttggccac ccggcctccc
     6841 ggccggatca ggcgggtgca gagctcgaag gtctcgggga cccccaccgc ctccacggcc
     6901 acgtccgcgc cgaggccgtc cgtgagatcc atgatctcct tctcgggatc ctgctgcccg
     6961 ttgttcacct gcacgcccgc cccgaactgc ttggcggcgt cgagccgcgc gtcggcaagg
     7021 tcgatcgcga cgatctggct cggggagaag agctgagccg tcaggatcgc agccagcccg
     7081 atcggtcccg cccccaccac ggccacggtg tccccggggc ggatccctcc gttcagcacg
     7141 ccgacctcgt atcccgtcgg gacgatgtcg gcgagcatga ggacggcctc gtccgacacc
     7201 ccctcgggga tgggatgggt ggacgtgtcc gcgaacggaa ctcggacgga ctcagcctgc
     7261 gttccgtcga tcaggtgccc gaggatccac ccgcctccac cgatgcagag gccgtaccgg
     7321 ccctcccggc agaagcggca gcggccgcat gccgtgatgc acgagacgag cacgcgatct
     7381 ccgggctgga gcgtggagac ccccgagccc acctcctcga cggtgcccac agcctcgtgc
     7441 ccgaggatgc ggccgtcggt gacggcgggc acgtccccct tcaggatgtg gaggtccgtg
     7501 ccgcagatcg tgaccgcgtc cacgcggacg atcgcgtccg tcgggtcctg gatctcgggg
     7561 ttcgggacct cctcccatgc cttctcgccg gctccgtgaa acaccatggc tcgcatcgcc
     7621 gacctcctct cagagacgcc tcctcgaccc cttacccgct gaggggggcc ctcgcacctg
     7681 cgatccgcgc gtcgggagcg acgaaggtca cgtcgtcgtc gggacctacg gcccacaaag
     7741 cgtcccggcg ggcggcacta ccgtggcgag gtcgtcgatg cgaggagcag aacgatggga
     7801 acccaccgtt ggctgggggt cggcctcatc acgctcgggg cgggcctcat cgtgagcgcg
     7861 atcctggggc cgctggtcct cgggatcgtc gactaccccc tgtcggagtc cctgacgaac
     7921 cagacgctcg gcctcgacgc ggtctccctg accctggtgg cgccggtcag tgtcctcgca
     7981 ggggcgctgg ccctgcgcgg ccatcccgcc gggcccgtgc tcggcttcgg gcccgccgcc
     8041 tacggctggt acatgctgct ccagttcgtg gtggggcccg agtacgcgta ctacccgggc
     8101 gcgctcccgc tccacctggg gctgtttgtc ctgagcggca cgatcgccgt ggggtcgtgg
     8161 agggcgatcc ggagcgagga gctgccgccc gcgacccggc gagccgatcg cctgcgtggg
     8221 gtggccctcc tggccttctc gggcttcctg ctctcgcggt acgtgcccct cgtggccgac
     8281 gcgctcaggg gcgaggggct gtcggccgag gcgctcgagg gcgtgacgat gttctggtcg
     8341 atcttcctcc tcgacctcgg ggtcgtgctc cccgcgacca tcgccgcggc cgtgggggtt
     8401 atcgccggcg cgccgtgggg gaggaaggcg atgtacgcgc tcgtgggctg gtgggccttc
     8461 gtcccggcct cggtggcggc gatggcgatc gtgatggtgg ggaacgacga cccgaacgcg
     8521 tcgacggggc aagccgccgt gttcgtggtg gtcgcggtgc tggtcctggc gttcgccgcg
     8581 gttgtctacc gcgggctgtt ccgggcggat cgcgtggctt cggcgcccgc gggtgaagcc
     8641 agccggaggc acctcaccag ggtcgggtag gcgtcagctc ccgaaggtca ctcgagccgt
     8701 ctcgcatcca cggatcaccg ccgcgagctc ggcctcgccg gtcacctcct cccggacggc
     8761 gctcgtgagc cgcccgaagg gctcgaccgt cacctcgagt cgtcggccct tgatcgtcga
     8821 gcgccacgtg gcgacgagct cgccggcgga gagaactccc cccggcccgc ggatcggctg
     8881 ccagagccgc ttccgatggg ccgcgtccgc cagcagcgtg gcgcgatcac gctgctgcag
     8941 ataggggtcc tggacgggga gcaggcggac gccccttggc tcgctcgggg cggagagggt
     9001 cttcgcgtcg cggcggagca tccaggacct cccgtcgaag ctcacctcct ccagctctcc
     9061 ctcgatcagc gagaaggcct gcttcgcgtc ttccgtgctc cgctgggtcc actcggcgaa
     9121 acggcgcggg ctgctcggcc cgtaggcgtg gaggaagcgg cgggccagct cggcccgagc
     9181 ttcctccttc gaggccgggt cgggcgagga tcccagccac tgatccgtgc gaccgagctc
     9241 cgagctggtg ccccggccgc tcacgatgca gaccgccccc gccgtcccca gggcccggaa
     9301 caggggctcg ggcatcgggt cccgggcgaa ctccggcttc cggaccctgg ccagcgccgg
     9361 cacgcgctcg cggaggagat cccggaactc gttgacgttc atggtccggc cgtcgaggag
     9421 gtcgcgggcg gcggccacca tctcgtcgag ggtctcgtga acgtcgaaac ccgcattctc
     9481 cagggcgtcg gaccacccgc ccatcgaccg gcggaaggag gcggcgtcga gaggcagggc
     9541 tcccagggtg aagacctcga gatcggacgc cggcacgatg tggggggcgc cccgcatgct
     9601 ccagatcgcc agcagcgtgc ggcgcacccg cagggcccga tcgagagcct cgggg
//
LOCUS       DASUTW010000286        13134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_484138, whole genome shotgun sequence.
ACCESSION   DASUTW010000286 DASUTW010000000
VERSION     DASUTW010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13134
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484138"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            401..2755
                     /locus_tag="VF058_09705"
     CDS             401..2755
                     /locus_tag="VF058_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX6208613.1"
                     /translation="MTVRSGRRALVALLAAMALAPGLAPATAQVSQGTWWVTEPEPDA
                     AENNGFIPYYEIGPRLREIETSSNRVKVEVIGQSVEGRNLFLVTVSDPQAMGRLGQYK
                     ALRNLMLRDPERAQEMAAEFQDFKVPFYVHGSIHGNEYPGVDASIELIERLAYENDPE
                     TRMVLDNTILLFNVVANPDGRVAGRRTNSNGFDLNRDFITQSQPETKAAVEVITEWNP
                     MVTLDLHGFVNPMLIEPCTPPHNNNYEYDLYIKWALDQAKAMEQELADDLGYSAQIPF
                     RDFPEAWDDWPPVYTPMYAMYHGSYGHTLETPFRDERGTAAHVTAVWGALKFASQNRM
                     GMIHDQIEIFKRGFLDMDQVSIADEYGEVHDFVAEFPRAHVIPVGEGQRSDAEAANLV
                     RFLVANDVQVERATGPVTVGSETYEAGSYVVYMDQPKRGLANTILGPGWDISDVVSRL
                     YSAPGAWSLGYLWGADVVTAERDQAFDAKTVPVNGASAQGGVDSGKAAAYALEVDSPT
                     AVRAVNALQSEGLSLELATEPVDSSGGTLAPGTVLIPSGAKNRLNQLAQEGLRFRAVA
                     SLPEREALEPVRIAGRVSDLELWVLRELGFDVDQVTGGQIASGAVDLAEYDVLLVTLT
                     NFWNQLGTAGRAAVQAFWEDGGGFVGVRSGGGQVANSSGHFPVSWISRSGTGMARLSY
                     EQDSPVVSAYPPEDTAQVETPTWFTSVPAGASVDARFLDEDFFVAGHWQDRSGAAGQP
                     LIVRGNGTFGDARVVLFGIDPLFRAHPQRTFPAVASSVYWTSAG"
     gene            complement(2814..3377)
                     /locus_tag="VF058_09710"
     CDS             complement(2814..3377)
                     /locus_tag="VF058_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208614.1"
                     /translation="MRRLVLHVLATAMVLGLAPALPANADDVVCRGVIGPKTVDDNIR
                     VPRGATCTLKGTTVGGNVFVKRGAILRAIGARIEGNVQAKRAKNVIIRARAFVDGDVQ
                     MERGRRGRVVRSRVEGNIQLKAQRGRLRVVRNVVDGDVQAFSNRGGVVIRGNRIDGNL
                     QCKSNRPAPVGGNNRVSGNKEGQCKRL"
     gene            complement(3504..4913)
                     /locus_tag="VF058_09715"
     CDS             complement(3504..4913)
                     /locus_tag="VF058_09715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6208615.1"
                     /translation="MTEQVDVVVLGLGTGGEEIGGRLAMAGLDVVGIEPELIGGECAY
                     WACLPTKMMIRAANLLAEARRVNEMSGRAEVTPDWSRVAARVREQVTGGWDDSMAAAR
                     FEGRGGTLVRGRGRFMGPRTVSVGDRTFAAERGVVIATGSVPRIPQIPGLDEVDFWTN
                     RDAIQVEELPASLLILGGGAIGCELGQVFSRFGVDVTIVEAGDRLLRSEEPEASEVIA
                     SVFDAEGIRVRTGSRAERVASRNGSVVATLGTGEELTAEALLVAVGRVVHADGLGLEE
                     AGVALSNGSIEVDEHMRAADGVWAMGDVTGQGMFSHMALFQAPIVEAGILGRTPPSAE
                     SSSRTRGTFTDPEIGTVGLTEDEARAEGRDVVVKVKQVPATFRGWLHGPGNEGVIKLI
                     ADRGTGTLVGATAVGPRGGDVLGFLAAAVHARIPIEELRRMIFAFPSFYAGVGEAIGA
                     YARALVQVLDPDAEPFLDV"
     gene            5072..6463
                     /gene="fumC"
                     /locus_tag="VF058_09720"
     CDS             5072..6463
                     /gene="fumC"
                     /locus_tag="VF058_09720"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014327027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX6208616.1"
                     /translation="MERRTETDSMGPIEVPADRYWGAQTQRSLHHFSIGDDRMPTEVV
                     HALGILKKAAALVNMDLEKLDRERGELIVRAADEVIEGTLDDHFPLFVWQTGSGTQSN
                     MNANEVIANRAIELAGGELGSKDPIHPNDHVNMSQSSNDTFPTAMHVAAARAVTERLL
                     PSLRALRDALDAKAKEFSDIVKIGRTHLQDAVPLTLGQEFSGYVAQLDDDLKRIEEAL
                     DGLHELAIGGTAVGTGLNAPEGFGEMAAARIAELTGLPFRAAPNAFAALAAHDALVHA
                     SAALRTLAVSLMKIANDVRWLASGPRSGLGELELPENEPGSSIMPGKVNPTQSEALTM
                     VCVQAMGNDAAVAIAGSQGNFELNVFKPVMIRNVLHSVAILADAMGTFREFCVEGIRP
                     NRERIAELVDRSLMLVTALTPKIGYDKAAEIAKKAHAEGSTLKEAALSLGYLTQEEYD
                     QAVRPERMVGPDA"
     gene            6460..7374
                     /locus_tag="VF058_09725"
     CDS             6460..7374
                     /locus_tag="VF058_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6208617.1"
                     /translation="MSDPVVSVDGLRKRYGDTIAVEDVSFEVRRGEIFGLLGPNGAGK
                     TTTVECLQGLRAPDAGSLHVLGLDPTHQAAELRRRIGSQLQQSALPDRIKVREALHLF
                     GSGADGTEAERLLLEWGLAEKRGARFQALSGGQRQRLFIALALLNQPEVVFLDEMTTG
                     LDPGARRVAWQLISAIRERGTTVVLVTHFMDEAEHLCDRVAIVDRGRIGALGSPQELI
                     VRHAGAVRVRFTAELPDASFLETIDGVTSVASRGSEFAVDGSGAVLARVAAALSSRGI
                     EPEDLRVERSTLEEVFLALVGTSDGMGR"
     gene            7371..8114
                     /locus_tag="VF058_09730"
     CDS             7371..8114
                     /locus_tag="VF058_09730"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6208618.1"
                     /translation="MRALVRTWWIEMKLFAREPLTVVFTLALPVVMLFVLASVFGNTP
                     DESVYRGVGPIDYYVPAYAALVTASLGLIALPTHLAEYRERGILRRFRASTVPLWTVF
                     AAHALVAVVASAVGSALLIAVAAAAYDIAAPVSPALFVGAFALGALTFAAIGGLLGAA
                     MPTTRAAQGAGVLLWFVMLMIAGAGPPPEVLTDATRAVADATPLRHVILVMQDPWLGF
                     GWNGTQTLIVLAWLAGSALLAGLLLRRRV"
     gene            complement(8116..8520)
                     /locus_tag="VF058_09735"
     CDS             complement(8116..8520)
                     /locus_tag="VF058_09735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX6208619.1"
                     /translation="MALDLEGIAGKLQDEVVVWLTTVSPEGQPQSVPVWFLWRDDAFL
                     IFSQPDKPKLRNLAANPRVSLHFRGTRNGEDIATLEGEARRAEDHPPADQVPPYVEKY
                     AAEIEKLRWTPQSFAADYSQPILVRPTRVLTW"
     gene            8585..10258
                     /locus_tag="VF058_09740"
     CDS             8585..10258
                     /locus_tag="VF058_09740"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX6208620.1"
                     /translation="MQGEREPRAPRWGWILVAAATAAAGAYLAPDPWTEGEGRLEVTL
                     RGAEPVEVPVEVGPGEPVEARAGRVELLVETGFPVDEPIEATVRVPGERPSLGGVSLA
                     LVLPDGARETIPILRREDDALVAGRRPPVGSAAVMGALGAVVILWVTEAIPLFVTSLA
                     IPVALVIAGAEAADALSPFFHPIIALFFAGFLMAEAMRRTRLDRLAAISIVARAGRSP
                     ATLFAAVLGVAAFLSMWMSNTAAVAVLIPIALAVTEPLDSPGYGRAVILGLAYAATIG
                     GVGSAIGTPANPLAIEFLGDFVGRRITFVGWFAYGLPMVVVFLPILGVYLWLRYRARP
                     DAERFEDARRAARRELAETGPLTRDQLVVLAVFAGVVALWLTQQWHGLDTGIVALMGA
                     IALAALGRILPSDLGRISWPSLLTFGGGLTLGFFLVESGTSDWMATRLGTLEGVPSWL
                     AIAVVAAVALALTTVASNTATAAMLIPLAIPLAGILGVDPVRLVIVVAIASSIDFALV
                     VGTPPTMLAYATGLYSTREIFRVGILLDVLGLTLLVTAVSAIWSLLGVV"
     gene            10312..11277
                     /locus_tag="VF058_09745"
     CDS             10312..11277
                     /locus_tag="VF058_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX6208621.1"
                     /translation="MRVAAAQTHPAWGDKEAGTKRVLEWIGRAADERVQLVAFGEAFL
                     SGYPFWVSRTDGARFDAADQKEAYAFYLEAAVELGGPELAQISEAVRDLGVFTYLGLT
                     ERGAGPARGTVYCTLVAIDPARGVVSAHRKLTPTYEERLVWGAGDGHGLRTHDVGPSR
                     VGGLNCWENWVPQARHAMYAQGEDLHVAVWPGSTGLTRDATRFVALEGRCFVLSAGAL
                     LSPGDVSDEFPLRGPALDRAPEVMYDGGSGVAGPDGAWVAEPVSGEERLVVADLDMSR
                     VREERQSFDPTGHYARPDVFEVRVHRRRLDAVTFDEDEGRSSGGP"
     gene            11277..>13134
                     /locus_tag="VF058_09750"
     CDS             11277..>13134
                     /locus_tag="VF058_09750"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6208622.1"
                     /translation="MPGDVADARERLRPYLPRLLVRWIEESPGEASREVAGSVAFADI
                     SGFTRLSERLAELGKLGAEEVAEAIDSSFTTLLAVAYEEGGSLIKFGGDALLLFFEDE
                     GHAVRAGRAAAGMRRALREMGAIRTPRGRVTLRMSIGVHSGTFHFFVVGESHRELVLA
                     GPGTTEVVAMETAAEAGEIVLSPATAAALPQRLLGEDKGPGRLLRRRPPGSPSGLPSI
                     DVTGSLEELARCLPVAVRTAVLGGSLDREHRLVTVAFLRFEGIDRVIEEEGPEAAATA
                     LAAVVEGVQRAADRHDLTFLGTDVDRDGGKIILASGTPLTVGNDEERILLAVREIVDA
                     DVPLPIRIGVHGGRVFAGDIGPAYRRTYTVMGDAVNLAARLMARAEPGQVISSRWVLR
                     RSRTIFRAERLPAFRVKGKEAPITACSVGSAEGAKAGSSQEHPFVGREREMTVLLEGL
                     ASARAGAGRVVEVIGDAGIGKSRLVRELRDRVGDAAVYAATCELYESQRPYRPFRDLL
                     RDVLRVPHDASEREVETTLRGLVERHAPQLLPWLPLLALPLHVDLPPTREVEELEERF
                     RRERVEQATGELLGALLSGPTMITIEDAHWMDEASASLLSHLVRTVADRPWLL"
BASE COUNT         1816 a         4741 c         4655 g         1922 t
ORIGIN      
        1 gcgacgaccc cgagcgcggt ctcgttcacg atgaacctcc tgtgcaaacg acggtgcgct
       61 cggtacgagg gctgcggtcg ctgccgtggg caggaggagc gcacccgcaa ccgcatccag
      121 tctatcaacc cgcggagaaa gagtgacaag acgagcgtcc cgggttgcgc ccggacgccc
      181 ggacaatgta caaacgctcc ggaaccgctg gggtcttcgg gcctcggcgg ttccgtcatc
      241 tccgacccga tttccctcgg ccggccccgc tggtttaccc tccggtcgaa cccgcagccg
      301 ccatgatcga gcgccccgag atcgccgacc gagacccctt caccggccgc cggacggcat
      361 ggctcgatcg gacgtcggct tccgaccaga ggaggttgcc atgacggtac gctcagggag
      421 acgcgccctc gtcgcgctgc tggcggcgat ggcgctcgcg ccgggcctgg caccggccac
      481 cgctcaggta tcgcagggca cgtggtgggt gaccgagccc gagcccgacg cggccgagaa
      541 caacggcttc atcccgtact acgagatcgg gccccgcctg cgcgagatcg agaccagcag
      601 caaccgggtg aaggtcgagg tcatcgggca gtccgtcgag ggccgcaacc tgttcctggt
      661 cacggtctcg gacccgcagg cgatggggag gctgggccag tacaaggccc tccggaacct
      721 gatgctccgc gacccggagc gggcccagga gatggccgcc gagttccagg acttcaaggt
      781 ccccttctac gtccacgggt cgatccacgg caacgagtac ccgggcgtgg acgcatcgat
      841 cgagctgatc gagcgcctcg cctacgagaa cgaccccgag accaggatgg tgctcgacaa
      901 cacgatcctg ctcttcaatg tggtggcgaa cccggacggt cgcgtcgccg gtcggcgcac
      961 caactcgaac gggttcgacc tcaaccgtga cttcatcacg cagtcccagc cggagacgaa
     1021 ggccgcggtg gaggtcatca ccgagtggaa cccgatggtg acgctcgacc tgcacggctt
     1081 cgtgaacccg atgctgatcg agccgtgcac gccgccgcac aacaacaact acgagtacga
     1141 cctgtacatc aagtgggcgc tggaccaggc caaggcgatg gagcaggagc tcgccgacga
     1201 cctcggctac tcggcccaga tcccgttccg cgacttcccg gaggcctggg acgactggcc
     1261 ccccgtgtac acgccgatgt acgccatgta ccacgggtcg tacggccaca cgctggagac
     1321 ccccttccgg gacgagcgcg ggactgccgc ccacgtcacc gcggtgtggg gcgcgctgaa
     1381 gttcgcctcc cagaaccgga tggggatgat ccacgaccag atcgagatct tcaagcgagg
     1441 cttcctcgac atggaccagg tctcgatcgc cgatgagtac ggcgaggtgc acgacttcgt
     1501 cgcggagttc cctcgggcgc acgtgatccc cgtcggggag ggccagcgga gcgacgcgga
     1561 ggccgcgaac ctcgtgcggt tcctcgtggc gaacgacgtc caggtcgagc gggccacagg
     1621 gccggtgacg gtggggagcg agacctacga ggcgggctcc tacgtcgtct acatggacca
     1681 gccgaagcga gggttggcca acaccatcct cgggccggga tgggacatct ccgacgtcgt
     1741 gtcccgcctg tactcggcgc cgggagcctg gagcctcggg tacctgtggg gggccgacgt
     1801 ggtcacggcc gagcgggacc aggccttcga cgcgaagacg gtcccggtga acggcgcctc
     1861 ggcgcagggc ggcgtggact cggggaaggc cgccgcctac gcgctggagg tggacagccc
     1921 caccgccgta cgggcggtga acgcgctgca gtcggagggc ctgagcctgg agctcgccac
     1981 cgagccggtc gactcctcgg gcggcacgct ggcgccgggg acggtgctga tcccgtccgg
     2041 ggccaagaac cgcctcaacc agctcgctca ggagggactg cggttccgtg cggtggcctc
     2101 gctgcccgaa cgcgaggcgc tcgagcccgt ccgcatcgcc gggcgcgtct cggacctcga
     2161 gctctgggtc ctgcgagagc tcggattcga cgtcgaccag gtgaccggcg ggcagatcgc
     2221 gagcggcgcc gtggacctgg cagagtacga cgtgttgctc gtcacgctga cgaacttctg
     2281 gaaccagctg gggacggccg gccgagccgc cgtccaggcg ttctgggagg acggcggggg
     2341 cttcgtcggc gtccgctccg gcggcgggca ggtcgcgaac agcagcggcc acttcccggt
     2401 gagctggatc tctcggtccg gcaccggcat ggcgcgcctg agctacgagc aggacagccc
     2461 cgtggtgagc gcctacccgc ccgaggacac ggcccaggtc gagaccccga cctggttcac
     2521 gtcggtcccg gccggcgcct cggtggacgc gaggttcctc gacgaggact tcttcgtggc
     2581 cggccactgg caggaccgct cgggcgcggc ggggcagccg ctgatcgtcc gcgggaacgg
     2641 gacgttcggc gacgcccgcg tcgtgctgtt cgggatcgat ccgctgttcc gggcccaccc
     2701 gcagcgaacc ttccccgctg tggcgtcctc ggtgtactgg acgtcggcgg gctgagcgag
     2761 aagggggcgg cggcggcccc gcgatgtggg gccgccgccc cccctccttc aggctagagg
     2821 cgcttgcact gcccttcctt gttcccgctc acgcggttgt tcccgccgac cggagccggc
     2881 ctgttcgact tgcactgaag gttcccatcg atgcggttgc cgcgtatcac gaccccaccc
     2941 cggttcgaga acgcctggac gtccccgtcc accacgttcc tcacgaccct cagccgcccg
     3001 cgctgggcct tgagctggat gttcccctcg acgcgagagc gcacgaccct tcccctgcgc
     3061 ccacgctcca tctggacgtc gccgtccacg aaggccctgg cccggatgat cacgttcttc
     3121 gcccgcttgg cctggacgtt gccctcgatg cgggcgccga tcgcccggag gatcgctccg
     3181 cgcttgacga agacgttccc tcccacggtc gtgcccttca gggtgcaggt cgctccccgc
     3241 ggaaccctga tgttgtcgtc caccgtcttc ggtccgatca ccccccggca taccacgtca
     3301 tcggcgttcg ccggcagggc gggcgccagg cccaggacca tcgccgtcgc cagcacgtgc
     3361 agcaccagtc tgcgcatcga cttcctccct caggttgacc tcgatcacac tctctcctca
     3421 cccggtgagt cgatcgtgag acccacgtga gaccgtcctc atcaggcaga cggccgccgc
     3481 ggcgtctcgg accggccggc cgactacacg tcgaggaagg gctccgcgtc ggggtccagc
     3541 acctgcacca gcgcccgcgc gtaggcgccg atcgcctcgc caacgcccgc gtagaagctg
     3601 gggaaggcga agatcatccg ccggagctcc tcgatcggga tccgagcgtg gacggcagcc
     3661 gccaggaacc cgagcacgtc gccgcctcgc ggcccgacgg ccgtggcgcc gacgagcgtc
     3721 ccggttccac ggtcggcgat cagcttgatc accccctcgt tcccgggccc gtggagccac
     3781 ccacggaacg tcgcgggcac ctgcttcacc ttcacgacga cgtcccgccc ctccgcgcgg
     3841 gcctcatcct cggtcagccc caccgtgccg atctccgggt cggtgaacgt ccccctggtg
     3901 cgcgaggagg actccgccga aggcggcgtc cttcccagga tccccgcttc gacgatcggc
     3961 gcctggaaga gcgccatgtg gctgaacatc ccctggccgg tgacgtcgcc catggcccag
     4021 acgccgtcgg cggcccgcat gtgttcgtcc acctcgatcg acccgttcga gagcgcgacg
     4081 cccgcctcct cgagcccgag gccgtccgcg tgcacgaccc gccccaccgc cacgaggagc
     4141 gcctcggctg tgagctcctc ccccgtcccg agggtggcga ccacggaacc gttccgggac
     4201 gccacccgct cagcgcgaga gcccgtgcgc acccggatgc cctccgcgtc gaacacggag
     4261 gcgatcacct cgctcgcctc cggctcctcc gaccgcagga gacgatcccc cgcctccacg
     4321 atggtgacgt cgacgccgaa ccgagagaac acctgtccca gctcgcaccc gatcgcgcct
     4381 ccgccgagga tgagcagcga ggcggggagc tcctcgacct ggatcgcgtc gcggttggtc
     4441 cagaaatcga cctcgtcgag gcccgggatc tgtgggatcc gaggcacgga tcctgtggcg
     4501 atcaccacgc cgcgctcggc cgcgaaggtc cgatcgccca ccgaaacggt cctcgggccc
     4561 atgaagcggc ctcgcccacg gaccagcgtc cccccgcgcc cctcgaagcg cgccgcggcc
     4621 atcgagtcgt cccacccgcc cgtcacctgt tcgcggacgc gcgctgccac acgagaccag
     4681 tcgggcgtca cctccgcccg cccggacatc tcgttcaccc gccgggcctc cgccaggagg
     4741 ttggccgccc ggatcatcat cttcgtgggc aggcacgccc agtacgcgca ctcgccgccg
     4801 atcagctcgg gctcgatccc gaccacgtcg agccccgcca tggccagccg cccgccgatc
     4861 tcctcacctc ccgttcctaa cccgaggacg acgacgtcaa cctgttccgt catgggatcc
     4921 tcacctccgt cggagagtcg atggcgtccc cggggggtgg ccgcgcccgc tcgcatcgcc
     4981 tgcgcctcgc cggggttccc cgatcatatc gggcgcgtcg agccggaggg agacgctcgc
     5041 tcccgtagaa tcgggccggg agcacatcgc gatggagagg cggacagaga cggacagcat
     5101 ggggccgatc gaggtgcccg ccgaccggta ctggggggcg cagacgcagc gctccctcca
     5161 ccacttctcg atcggcgacg atcggatgcc gaccgaggtg gtccacgccc tcgggatcct
     5221 caagaaggcc gcggcgctcg tgaacatgga tctcgagaag ctcgaccgcg agcggggaga
     5281 gctgatcgtc cgggccgccg acgaggtgat cgaggggacc ctcgacgatc acttccccct
     5341 cttcgtatgg cagacgggct cgggcaccca atccaacatg aacgcgaacg aggtgatcgc
     5401 gaaccgggcg atcgagctgg cggggggcga gctgggctcg aaggacccga tccaccccaa
     5461 cgaccacgtc aacatgtcgc agtcctcgaa cgacacgttc cccacggcga tgcacgtggc
     5521 cgcggcccgg gccgtgaccg agcgactgct cccgagcctc cgggccctcc gcgacgcgct
     5581 cgacgccaaa gcgaaggagt tctcggacat cgtgaagatc gggcggaccc acctccagga
     5641 cgcggtcccg ctcacgctgg gccaggagtt ctccggctac gtagctcagc tggacgacga
     5701 cctgaagcgg atcgaggagg ccctggacgg gctccacgag ctggccatcg gcgggaccgc
     5761 ggtcgggacc ggcctgaacg cgccggaggg cttcggcgag atggccgccg caaggatcgc
     5821 cgagctgacg gggctcccct tccgggccgc tcccaacgcc ttcgcggccc tcgccgccca
     5881 cgacgccctc gtccacgcga gcgccgccct ccggaccctg gcggtctccc tcatgaagat
     5941 cgccaacgac gtccgctggc tcgcctccgg gccccgctcc ggcctcgggg agctcgagct
     6001 ccccgagaac gagcccggct cctcgatcat gccgggcaag gtgaacccca cgcagtccga
     6061 ggccctgacg atggtctgcg tccaggcgat ggggaacgac gccgcggtgg cgatcgccgg
     6121 ctcccagggc aacttcgagc tgaacgtctt caagcccgtg atgatcagga acgtcctgca
     6181 ctcggtggcg atcctggccg acgcgatggg cacgttccgc gagttctgcg tggaggggat
     6241 ccggccgaac cgcgagcgga tcgccgagct cgtagaccga tcgctgatgc tcgtgacggc
     6301 cctgacgccc aagatcggct acgacaaggc ggccgagatc gcgaagaagg cgcacgccga
     6361 aggctcgacg ctgaaggagg ccgccctgtc gctcggatac ctcacccagg aggagtacga
     6421 ccaggccgtc cgccccgagc ggatggtcgg gccggacgcg tgagcgaccc cgtcgtctcc
     6481 gtcgacggtc tgcggaagag gtacggcgac acgatcgcgg tcgaggacgt cagcttcgag
     6541 gtccggcggg gtgagatctt cggactcctc ggcccgaacg gcgcggggaa gacgaccacc
     6601 gtggaatgcc tccagggcct ccgcgcgccc gacgcgggct cgctccacgt cctgggcctc
     6661 gaccccacgc accaggccgc cgagctgcgg cgtcggatcg gcagccagct ccagcagtcc
     6721 gcgctccccg accggatcaa ggtgcgcgag gcgctccatc tgttcggctc aggggccgac
     6781 gggacggagg ccgagcgcct cctcctggag tgggggctgg ccgagaagcg gggcgccagg
     6841 ttccaggccc tctcaggcgg acagcgccag cggctgttca tcgccctggc cctgctcaac
     6901 cagccggagg tcgtgttcct cgacgagatg accaccggcc tcgaccctgg ggcgcggcgg
     6961 gtggcgtggc agctgatctc ggccatccga gagcggggca cgacggtcgt cctcgtgacg
     7021 cacttcatgg acgaggccga gcacctgtgc gaccgcgtgg cgatcgtgga tcggggccgg
     7081 atcggcgcgc tgggctcgcc ccaggagctc atcgtccgac atgcgggcgc cgtccgggtg
     7141 cggttcacgg ccgagctccc cgacgcgtcg ttcctcgaga ccatcgacgg cgtgacgtcc
     7201 gtggcgtctc gcggcagcga gttcgcggtg gacgggtcgg gggccgtgct ggccagggtg
     7261 gccgccgcgc tctcgtcccg cgggatcgag ccggaggacc tccgcgtcga gcgctccacg
     7321 ctggaggagg tcttcctggc gctcgtcggc accagcgatg ggatggggcg atgagggcgc
     7381 tcgtccgcac ctggtggatc gagatgaagc tcttcgcccg cgagccgctc accgtcgtgt
     7441 tcacgctcgc cctgccggtc gtgatgctct tcgtcctggc cagcgtgttc ggcaacacgc
     7501 ccgacgagag cgtctaccgg ggtgtggggc cgatcgacta ctacgtgccc gcgtacgccg
     7561 cgctggtgac ggcctccctg ggcctgatcg ccctccccac gcacctggcc gagtatcggg
     7621 agcggggcat cctgcgccgg ttccgggcct ccacggtccc gctgtggacg gtcttcgccg
     7681 cccacgcgct cgtcgctgtc gtagcctcgg cggtcgggag cgctctgctg atcgccgtgg
     7741 cggctgccgc ctacgacatc gccgcgcccg tctcccccgc cctgttcgtc ggcgcgttcg
     7801 cgctgggcgc gctgaccttc gctgcgatcg gagggctcct cggggcggcg atgccgacga
     7861 cgcgagcggc gcagggcgcg ggcgtgctgc tctggttcgt catgctgatg atcgccgggg
     7921 ccggaccgcc tcccgaggtg ctcaccgacg ccacgcgggc ggtggccgac gccacgcccc
     7981 tgcggcacgt gatcctcgtg atgcaggacc cgtggctcgg cttcggctgg aacggcacgc
     8041 agacgctcat cgtcttggcc tggctcgcgg gatcggccct gctggcgggc ctcttgctgc
     8101 ggcgtcgcgt ctaggtcacc aggtcaggac gcgggtgggc ctgacgagga tcggctggga
     8161 gtagtccgcg gcgaagctct gcggggtcca tcggagcttc tcgatctccg cggcgtactt
     8221 ctccacatag ggagggacct ggtcggcggg tggatggtcc tcggctcgcc tggcctcccc
     8281 ctcaagcgtg gcgatgtcct cgccgttccg ggtgccccgg aagtgaagcg agacccgggg
     8341 gttggcggcc aggttccgaa gcttcggctt gtccggctga ctgaagatga ggaaggcgtc
     8401 gtcgcgccag aggaaccaga ccgggacgct ttgcggctgc ccctccggcg agacggtggt
     8461 cagccacacg acgacctcgt cctgcagctt gccggcgatc ccctcgaggt ccagggccac
     8521 gagcacagcc tagcgggaga ccgcgctcgc ggcgagggcg gtcggcaccg taggctcgat
     8581 atccatgcag ggcgagcgtg agccccgcgc gccgcgctgg ggctggatcc tggtggccgc
     8641 cgcaaccgcg gcagcgggag cctacctggc gccggacccc tggaccgagg gcgagggtcg
     8701 gctcgaggtg accctccggg gggccgagcc cgtcgaggtc ccggtggagg tcgggcccgg
     8761 cgagccggtc gaggcccggg ccggtcgcgt cgagctcctg gttgagaccg gcttcccggt
     8821 ggacgagccg atcgaggcca ccgtgcgcgt ccccggggag cgcccctcgc tggggggcgt
     8881 ctcgctcgcc ctcgtcctcc cggacggcgc cagggagacg atcccgatcc ttcggcggga
     8941 ggacgacgcg ctcgtggccg gtcggcggcc gccggtgggc tcggcggccg tcatgggggc
     9001 gctgggcgcc gtcgtgatcc tctgggtgac tgaggcgatc ccgctgttcg tcacctcgct
     9061 ggccatcccg gtggccctgg tgatcgctgg ggccgaggcg gccgacgcgc tctcgccgtt
     9121 cttccatccg atcatcgcgc tcttcttcgc gggcttcctt atggccgagg cgatgcgacg
     9181 gacccgcctc gaccggctgg ccgcgatctc gatcgtggcc cgggccggcc ggagccccgc
     9241 aacgctcttc gcggccgtcc tcggcgtcgc cgcgttcctc tcgatgtgga tgtcgaacac
     9301 ggccgcggtg gccgtgctga tcccgatcgc gctggcggtg accgagccgc tggacagccc
     9361 gggttacggg cgggccgtga tcctcggtct ggcctacgcg gccacgatcg gaggcgtggg
     9421 cagcgcgatc ggcacacccg ccaacccgct agcgatcgag ttcctgggcg acttcgtggg
     9481 gaggcggatc acgttcgtgg gctggttcgc ctacggcctc cccatggtcg tcgtcttcct
     9541 tccgatcctc ggggtctatc tgtggctgcg gtaccgggcc cggccggacg ccgagcgctt
     9601 cgaggacgcg cggcgcgcgg cgaggcggga gctggccgag acgggacccc tcacccggga
     9661 ccagctcgtg gtgctggcgg tcttcgccgg ggtcgtcgcc ctgtggctga cccagcagtg
     9721 gcacggactc gacacgggga tcgtggcgct catgggggcg atcgcgctcg cggcgctggg
     9781 ccgcatcctc ccctcggacc tcgggcggat ctcgtggccc tctctgctca ccttcggcgg
     9841 gggcctcacg ctggggttct tcctggtcga gtcggggacc tccgactgga tggccacgcg
     9901 gctcggcacg ctggagggcg tcccgtcatg gctggcgatc gcggtggtgg cggccgtcgc
     9961 cctggccctc accaccgtcg cgtcgaacac ggccacggcc gcgatgctga tccccctggc
    10021 catcccgctg gcggggatcc tcggcgtcga tccggtgcgg ctcgtgatcg tcgtggcgat
    10081 cgcctcgtcg atcgacttcg ccctggtggt cgggacgccg cccacgatgc tggcgtacgc
    10141 gacgggcctc tactcgaccc gggagatctt ccgggtgggg atcctgctcg acgtcctcgg
    10201 cctgacgctg ctcgtcacgg ccgtgtcggc gatctggtcg ctcctcgggg tcgtgtgacc
    10261 gtggcgggca cggccccgat ggggcacagt gagacccaga aggaggggga gatgagggtt
    10321 gccgcggcgc agacccatcc ggcgtggggc gacaaggagg ccgggaccaa gcgggttctg
    10381 gagtggatcg ggagggcggc cgacgagcgc gtccagctcg tggccttcgg cgaggccttc
    10441 ctctcgggct atcccttctg ggtctcccgg accgacggcg cgcgattcga tgccgccgac
    10501 cagaaggagg cctacgcgtt ctacctcgag gccgcggtcg agctgggcgg cccggagctg
    10561 gcccagatct ccgaggccgt ccgcgatctc ggcgtcttca cgtacctcgg cctcaccgag
    10621 cgcggcgccg ggcccgcccg cggcaccgtg tactgcacgc tcgtggcgat cgatccggcc
    10681 cgcggcgtcg tgagcgccca ccgcaagctc accccgacct atgaggagcg cctcgtctgg
    10741 ggcgccggcg acggccacgg gctccggacg cacgacgtcg ggccctcgcg agtcggaggt
    10801 ctgaactgct gggagaactg ggtcccccag gccaggcacg cgatgtacgc gcagggcgag
    10861 gacctccacg tcgccgtctg gcccggctcc accgggctga cccgggacgc cacgcgcttc
    10921 gtggcgctcg agggccgctg cttcgtgctc tccgctgggg ccctcctctc gccgggggac
    10981 gtgagcgacg agttcccgct gcgggggccg gcgctggata gagccccgga ggtgatgtac
    11041 gacggcggct cgggcgtcgc cggcccggac ggcgcgtggg tcgcggagcc ggtctccgga
    11101 gaggagcggc tcgtggtggc cgacctcgac atgagccgcg tgcgcgagga gcggcagagc
    11161 ttcgacccca cgggccacta cgccaggccc gacgtgttcg aggtccgcgt gcaccgccgc
    11221 cgcctggacg ccgtcacttt cgacgaggac gaggggagaa gctcgggagg gccgtgatgc
    11281 cgggcgacgt ggccgacgcc cgcgaacgcc tccgtcccta cctgccccgg ctcctcgtgc
    11341 ggtggatcga ggagtcgccg ggcgaggcga gccgcgaggt ggccgggtcc gtcgccttcg
    11401 ccgacatctc cgggttcacc aggctctccg agcggctcgc cgagctcggg aagctgggcg
    11461 cggaggaggt cgcggaggcg atcgactcct cgttcaccac gctgctggcc gtcgcctacg
    11521 aagagggcgg cagcctgatc aagttcgggg gcgacgccct gctcctcttc ttcgaggacg
    11581 aggggcacgc ggtccgggcc ggcagggccg ccgcggggat gcgtcgggcc ctccgcgaga
    11641 tgggagcgat ccgaacgccc cggggacggg tgaccctccg gatgtcgatc ggcgtgcaca
    11701 gcggcacctt ccacttcttc gtggtgggcg agtcccaccg cgagctcgtg ctcgccggcc
    11761 cgggcaccac cgaggtcgtg gccatggaga ccgccgccga ggcaggggag atcgtgctga
    11821 gccccgccac ggccgccgcg ctgccacagc gcctgctggg cgaggacaag ggccccggac
    11881 gtctcctgcg gcgccggcct cccgggtccc cgagcggcct cccatcgatc gacgtcacgg
    11941 gctccctcga ggaactcgcc cgctgcctcc cggtcgccgt gcggacggcc gtgctcggcg
    12001 gctccctcga ccgggagcac cgcctggtca ccgtcgcctt cctgcggttc gagggcatcg
    12061 accgcgtgat cgaggaggag gggccggaag cggcggccac ggcgctggcg gccgtggtgg
    12121 agggggtcca gcgcgccgcc gaccggcacg acctgacctt cctgggcacc gacgtcgacc
    12181 gcgacggggg gaagatcatc ctcgcctccg gcacgcccct gacggtgggg aacgacgagg
    12241 agcggatcct gctcgccgtc cgcgagatcg tggacgcgga cgtgccgctc ccgatcagga
    12301 tcggggtgca cggaggacgg gtcttcgccg gcgacatcgg ccctgcctac cgccgcacct
    12361 acaccgtgat gggcgacgcg gtgaacctcg cggcgaggct gatggcgaga gcggaaccgg
    12421 ggcaggtgat ctcgagcagg tgggttctcc gccggtcgcg cacgatcttt cgcgccgagc
    12481 ggctcccggc gttccgggtc aagggcaagg aggcgcccat caccgcctgc tccgtgggat
    12541 cagccgaggg ggcgaaggcc gggtcctccc aggaacaccc gttcgtgggc agggagcggg
    12601 agatgaccgt gctgctcgag gggctcgcat cggccagggc gggcgcgggc cgggtggtcg
    12661 aggtgatcgg cgacgcgggc atcgggaagt cgcggctcgt ccgggagctc cgggatcggg
    12721 tcggcgacgc cgcggtctac gcggcgacct gcgagctgta cgagtcgcag aggccctacc
    12781 gaccgttccg cgacctcctc cgcgacgtgc tccgggtccc ccacgacgcc tccgagcgcg
    12841 aggtcgagac caccctccga gggctggtcg agcggcacgc cccccagctc ctcccctggc
    12901 tgccgctcct ggcgctcccg ctccacgtcg atctcccccc cacccgagag gtcgaggagc
    12961 tcgaggagcg cttccgtcgg gagcgggtgg agcaggccac gggcgagctc ctgggcgcgc
    13021 tgctctccgg gccgacgatg atcacgatcg aggacgccca ctggatggac gaggcctccg
    13081 ccagcctcct ctcgcacctc gtccgcacgg tcgcggaccg tccgtggctg ctcg
//
LOCUS       DASUTW010000287         2511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_873862, whole genome shotgun sequence.
ACCESSION   DASUTW010000287 DASUTW010000000
VERSION     DASUTW010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873862"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..632
                     /locus_tag="VF058_09755"
     CDS             117..632
                     /locus_tag="VF058_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX6208623.1"
                     /translation="METWDAIRSRRNVREYQDRPIPPEHLDRILEASRRTPSSQNLQR
                     WEFVLVTDRERLRELSGVWRGAGHVGRAAAAIALLAPETADGREATSIHYDLGQATMS
                     IMLAAADLGVGSSHAGVADRGLAQRVLGHPEDRSCRWIVALGYPKDRPLKPVERPDRR
                     PFEDVVRRERW"
     gene            complement(636..1529)
                     /locus_tag="VF058_09760"
     CDS             complement(636..1529)
                     /locus_tag="VF058_09760"
                     /inference="COORDINATES: protein motif:HMM:NF041045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX6208624.1"
                     /translation="MPRSPRLSHPVLFYADEDEFIAGTVPFLRRGIENDEVVFVAARP
                     DYLESVRVELGSDASAAIWKNTHVWHSHHASRLRAFYELVSGAPRGARLRLVGEPVWP
                     ERPEEVREWQRYESALNAVLAPFPVSLLCLYDAASLDPEIVETAGRTHPGDRYEEPDS
                     FLRAHRADPSSPPRTAEVVEQVRDPAPARGLALDRAIRAGVDPDAAVGLSIAVNEILT
                     NALLHGGGDARLSLWREGASFVCQVEDEGPGIADPLAGYRPPAHRTGGRGLWLARQLV
                     DLMEILPVEVGAAVRLCVPVG"
     gene            complement(1683..>2511)
                     /locus_tag="VF058_09765"
     CDS             complement(1683..>2511)
                     /locus_tag="VF058_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX6208625.1"
                     /translation="GYAADATIERFASPNRVTVTASPVRMTDNDECSPIWPWSCPHQA
                     TNDFDGYLDVLATDYGAWADRAQREAMYGMDYTTNVGLTSIPPEIVNDPATGRERLLI
                     RMANHHLYSDATLFEGFAHLRIPNLFLRRAYGVDHPSSLTGSGIDTAGTGSAATVSVM
                     PEPGGGAMLVDIEGMRFSRKRVRISRGVITPTKPKDVRARRTPKRVRLRFTEAKQRGS
                     KITRYRASCVRGGTALTGGAKRSPVVVKGLSPGVKYACRVRAISKAGPGRWSKKVRA"
BASE COUNT          369 a          912 c          883 g          347 t
ORIGIN      
        1 ccgcgtcgag ggcggcgagg agatgagcgg cgggccgacc gtctgaggcc ccggggcgcc
       61 gccgtcccat cgggacggcg gcggcccgcc tccctctacc ctctaggatc ctcctcatgg
      121 agacgtggga cgcaatccgc agcaggcgca acgtccgcga gtaccaggac cgcccgatcc
      181 ctcccgagca cctcgaccgc atcctggagg cgtcgcgccg gaccccatcg tcccagaacc
      241 tgcagcgctg ggagttcgtc ctcgtcaccg acagggaaag gctgcgggag ctgagcggcg
      301 tatggcgcgg ggccggccac gtcggccgag cggccgcggc gatagccctc ctcgcccccg
      361 agaccgccga tggacgggag gcgacctcga tccactacga cctgggccag gccacgatgt
      421 cgatcatgct cgccgccgcc gatctcgggg tggggagcag ccacgccggc gtcgccgacc
      481 ggggcctggc ccagcgcgtc ctgggacacc ccgaggaccg ctcctgccgg tggatcgtcg
      541 cgctcggcta tccgaaggac cgcccactca agccggtcga gcgccccgac cgtcgtccgt
      601 tcgaggacgt ggtccggcgc gaacgctggt agccgtcagc cgaccggcac gcagagcctg
      661 acggcggctc cgacctcgac cgggaggatc tccatcaggt cgacgagctg tcgggcgagc
      721 cagaggcccc ggccccccgt gcggtgcgcc ggcggccggt agccggcgag gggatcggcg
      781 atccccggcc cctcgtcctc gacctgacac acgaaggagg ctccctccct ccagagcgat
      841 aggcgggcgt ccccgccgcc gtggaggagc gcgttggtca ggatctcatt aaccgcgatg
      901 ctcaacccga ccgcggcgtc cgggtccaca ccggcccgga tcgcacggtc cagggccaag
      961 ccacgcgccg gggccgggtc acgcacctgt tcgaccacct cggccgtcct cgggggggat
     1021 gacggatccg ctcggtgggc gcggaggaac gaatcgggct cctcgtagcg gtcgccggga
     1081 tgcgtccgcc ccgcagtctc gacgatctcc gggtcgagcg aggccgcgtc gtagaggcag
     1141 aggagcgaga ccgggaaggg cgccagtacc gcgttgagcg ccgactcgta gcgctgccac
     1201 tcgcggacct cctccggccg ctcgggccag acgggctctc ccaccaggcg gagcctggca
     1261 cccctcgggg cgccgctgac cagctcgtag aacgcgcgca gccgcgaggc atggtgagag
     1321 tgccagacgt gcgtgttttt ccagatcgcc gccgacgcgt ccgagcccag ctcgacccgg
     1381 acggactcga ggtagtcggg tcgagccgcc acgaagacca cctcgtcgtt ctcgatccca
     1441 cggcggagga aggggacggt ccccgcgatg aactcgtcct cgtccgcgta gaacagcacg
     1501 gggtgcgaga gccgggggct acgaggcatc cccggtccac gccatggact ggcccggtcg
     1561 acgggtcatg ggcgcatctg ctcccggcct tcggggggcc ctctcgtgag cgaccggtcg
     1621 ctcataggcc tgcccctacc ctcgacccgg tccttccaca catggcccga ggcgctcgct
     1681 cgctaggccc gcaccttctt cgaccagcgg ccgggaccgg ccttggagat cgcccgcacc
     1741 cgacacgcgt acttcacgcc cggcgacagg cccttcacca ccacggggga gcgcttcgcg
     1801 ccccccgtca gcgcggtgcc cccgcggacg caggaggctc ggtagcgcgt gatcttggag
     1861 ccgcgctgct tggcctcggt gaagcgcaga cggacccgct tgggagtccg acgggctcgc
     1921 acgtccttcg gcttcgtggg cgtgatcacc ccgcgtgaga tgcggaccct cttgcgggag
     1981 aaccgcatcc cctcgatgtc gacgagcatg gcgccgccgc ccggctccgg catcaccgac
     2041 accgtcgccg ccgatcccgt acctgcggta tcgatgcccg agccggtgag cgaggacggg
     2101 tgatcgacgc cgtaggcgcg ccggaggaac aggttgggga tgcgaagatg cgcgaacccc
     2161 tcgaagaggg ttgcgtccga atagaggtga tggttggcca tccggatcag caggcgctcc
     2221 cgcccggtgg cggggtcgtt gacgatctcg ggggggatgc tcgtcagccc gacgttcgtc
     2281 gtgtagtcca tcccgtacat cgcctcacgc tgggccctgt ccgcccacgc cccgtaatcg
     2341 gtggcgagca cgtcgaggta gccgtcgaag tcgttcgtcg cctggtgcgg gcaggaccac
     2401 ggccagatcg ggctgcactc gtcgttgtcg gtcatccgca cgggagaggc ggtcaccgtc
     2461 acacgattcg gggacgcgaa gcgctcgatc gtggcgtcgg cagcgtagcc g
//
LOCUS       DASUTW010000288        24960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_873940, whole genome shotgun sequence.
ACCESSION   DASUTW010000288 DASUTW010000000
VERSION     DASUTW010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 24960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24960
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873940"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..417)
                     /locus_tag="VF058_09770"
     CDS             complement(49..417)
                     /locus_tag="VF058_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007272310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6208626.1"
                     /translation="MAAGMDIMRLASENEDFRREVITGEHGQVVLMTIAAGGEIGEEV
                     HDDVDQILLFVAGRGEAVLDGQTGPVGPNDMVFVRAGTRHNFRNVGDEPLRVVTVYAP
                     PEHPAGTVHRTKEEADAAEH"
     gene            complement(464..1261)
                     /locus_tag="VF058_09775"
     CDS             complement(464..1261)
                     /locus_tag="VF058_09775"
                     /inference="COORDINATES: protein motif:HMM:NF021615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HEX6208627.1"
                     /translation="MGERGLELRARTVGELLTDAFEVYRRNFLVLVSIAAVVVVPLTL
                     LQYLLFVGVADVWGAAPGGVRTLDASEAWRVSLGGLALGLISFVVSQLATGAGTHAVV
                     SAVAGDEPGVGLSYRAALGRLGSLLLATLLMGLAVGAGFVLFIIPGLIVLVRLTATVP
                     AVVVERATGGRAISRSWELVRGRSWPVFGAMLVVLLVTGILTGIVTRPVGDHWFAQGL
                     AASIASVLTAPYVVAVVVLLYLDLRVRKEGLDRTTLRHELVEGRGPA"
     gene            complement(1326..1460)
                     /locus_tag="VF058_09780"
     CDS             complement(1326..1460)
                     /locus_tag="VF058_09780"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX6208628.1"
                     /translation="MDGSVAGAWRYEGGRVRLEPFERLSRATMVEVEEEAERLAAFHA
                     "
     gene            complement(1541..2293)
                     /locus_tag="VF058_09785"
     CDS             complement(1541..2293)
                     /locus_tag="VF058_09785"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004595063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX6208629.1"
                     /translation="MTGWTPTEMMVVASARQLAGERVCFVGVGPPNIACNLARRTVSP
                     GLQLVYEAGVFGARPARLPLSIGDPSLATGSDAIHGMFELFAFYLQGGLVDVGFLGAS
                     QIDRHGNINTTVIGEYGAPKVRLPGSGGACEIAINARKVFVITSQSRRSFVERVDFTT
                     SPGHLAGRRPEGWIGAGPTVVVTDLGVHGFDASGEMELRSLHPGVPLDRVRAETGWEL
                     RVSEDLSETPPPTGEELRLIREELDPEGVATR"
     gene            complement(2290..3189)
                     /locus_tag="VF058_09790"
     CDS             complement(2290..3189)
                     /locus_tag="VF058_09790"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020667539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX6208630.1"
                     /translation="MPDAVRELVRDGDTVAIEGFTHLISFAAGHEIIRQGRRDLTLCR
                     LTPDLIYDQMVAAGCARRLVFSWLGNPGVGSLHAVRRRVEGREGDPLELEEYSHFGMV
                     CRYVAGASRLPFFPLRSYEGSDLPKVNPRIVPIESPYDPGERLYAVPPLNPDVVVVHA
                     QRADAEGNAQIWGLLGVQREAAFAGERVLVVCEELVEPEVIRADPNRTVIPGLIVDAV
                     VVAPRGCHPSFAQGYYDRDNRFYREWDAISRDPERLEAWLDEWVRGVSSHEEYAEKLG
                     EERWAELAPGEAWSGRVNYGSYE"
     gene            complement(3239..4147)
                     /locus_tag="VF058_09795"
     CDS             complement(3239..4147)
                     /locus_tag="VF058_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208631.1"
                     /translation="MELRREPISLGTVIEFVGYAGAAVALSAASTVLGQEAGEGVRIA
                     FDLITIVVLLAAGWAVAGKPEPYPRMQSVFWFVAVFSVADLAGLLFGEVIEGLDERMV
                     VFLTALVAALFSLVLWWVLRRSLQVLVLILTGYIAIISLVFPDVGDFILGPPDLSAVG
                     FVTWLLGGAVILLGWLGWLGPRRTSLAVGSILALIGPFFFLVGGEEIVGEILALATAF
                     ALMVLGSWIEDRGVAGLGIAGTLIVASTIVANHVEDEGPAIAVLVVGLLLLGAAILVA
                     RGTVGGPPALEEGEVPPPPPPAPPAP"
     gene            complement(4259..5062)
                     /locus_tag="VF058_09800"
     CDS             complement(4259..5062)
                     /locus_tag="VF058_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6208632.1"
                     /translation="MSTLAMVLGDGLVVAKRNLIKIKRVPEILIWTTMSPIMFVLLFA
                     YVFGGSIDTGNVSYREFLIAGVFVQTVIFGSTFTGAGLADDITKGIIDRFRSLPMSRS
                     AVLVGRTTSDILYNSISIAVMSIAGLIVGWRIRTSLLPAIGGFLLLLLFAYAVSWIMA
                     FVGLKVSSLEVFQNASFMFIMPVTFVANTFVLSESLPGPLRVFAEWNPVSAVAQAVRE
                     AFGNTATNVPTPEAWSLQNPVLYGLIWVGIILLIFVPLSIRQFRKAATR"
     gene            complement(5059..6069)
                     /locus_tag="VF058_09805"
     CDS             complement(5059..6069)
                     /locus_tag="VF058_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011029976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6208633.1"
                     /translation="MIRTSSLVKRYGKVTALDGLDLEVPKGTVLGLLGPNGAGKTTAV
                     RILTTLLDADEGTAEVAGLDVRTQATEVRRVIGLSGQTAAVDEYLTGYENLEMVGRLY
                     HMGGPRSRARARELLEQFDLEEAGNRPAKTYSGGMRRRLDLAAALVAEPEVLFLDEPT
                     TGLDPRSRVQMWDTIENLAQGGSTVLLTTQYMEEADRLADDIVVIDRGQKIAHGTADQ
                     LKLQVGGERLELVLGDMADLESARSILETVCTDEVQVTEHTRSLTTAVRGGSEDLRRV
                     LDLLQQRSVQIDDIGLRRPTLDDVFLTLTGHSAGAADGGNGDGQMPDAERIQEQEKEV
                     AR"
     gene            complement(6149..6637)
                     /locus_tag="VF058_09810"
     CDS             complement(6149..6637)
                     /locus_tag="VF058_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020522255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6208634.1"
                     /translation="MGNQLQVSTPTDREIVMTRVFDAPRDLVFEAHTSCEHMRNWWGP
                     RAMEISECEVDFREGGTWRIVHRGPDGEEYGFSGEYREIVRPERITWTFEFDGAPGSV
                     SVETLTLEEHDGKTTVTATAVYDSKEARDAMIESGMETGAAETYERLDEYLMTLGERA
                     AS"
     gene            complement(6603..6986)
                     /locus_tag="VF058_09815"
     CDS             complement(6603..6986)
                     /locus_tag="VF058_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020472998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6208635.1"
                     /translation="MSTATRERLDATFSALADPTRRAILSRLSEGEASVTQLAAPFDM
                     TLPAVSKHLKVLERAGLIARSRERQWRPARLAAEPLREAAAWLDRYRRFWEESYDRLD
                     EYLDELQGRRKEEEDGEPAAGLDPH"
     gene            complement(7156..7740)
                     /locus_tag="VF058_09820"
     CDS             complement(7156..7740)
                     /locus_tag="VF058_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020515904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX6208636.1"
                     /translation="MDAIYFADADGFRAWLEAHHATATELWVGFHKKQVGVPSMTWEE
                     SVDVALCFGWIDGVRRSVDEKRYVNRFTPRRPGSLWSARNVERARALIDRGLMAPAGL
                     AAFRARREDRSPYSYERRDSIRLDEDLERRFRRSSKAWERFQAMAPSYRKSALHWVMS
                     AKRQETRERRLERLIEDSAAGRKVPPLAPGKREG"
     gene            7943..9223
                     /locus_tag="VF058_09825"
     CDS             7943..9223
                     /locus_tag="VF058_09825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELWxxDGT repeat protein"
                     /protein_id="HEX6208637.1"
                     /translation="MKDIKPGPGSSLNDEGIDFRRVGRWLLFRADDGVAGSELWRTDG
                     TPKGTRRVKDINPGPGHSSPAEFELMGGVLFFQADDGVTGTELWRTDGTRKGTRRVKD
                     INPGPADSSPQELTRLGKRILFTANDGAAGWELWRTDGTKKGTRRVKDINPGPSFAQP
                     HDFVRLGKKLLFSASDGTLSIELWRTDGTKKGTRLVKDIRPGPGDSSPGDLVRLGKRV
                     IFSATHDATGRELWRTDGTRKRTRLVKDLFPGGNAFPERLTVYGKVILFRADGPAGRE
                     LWRTDGTRKGTRRVKDINPGPGDSEVDDLVRFGKIVLFQADDGVTGTELWRTDGTKKR
                     TKRLKDIHPGPGSSIPDKFTTVGDWAVFKADDGTTGAELWRTDGTKKGTRRLRDLNPG
                     PGAAYPGYFTPLGRWFLFGAYDGVTGYELWKLRR"
     gene            9237..10703
                     /locus_tag="VF058_09830"
     CDS             9237..10703
                     /locus_tag="VF058_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX6208638.1"
                     /translation="MTDQLRLADLLVAISVATDLGMGQEPEKAARSCLLATRMARGLG
                     LPEAEVREVYLATLLRHLGCTATAHEEAHLFGGDELASRPPSEAADFGEAREMLKLTL
                     ATGRGAGLRRPRLLARALLAGRKESEGILRAICEVGSHLASRLGLGHAVERALYQVTE
                     RWDGTGTPEGAAGEEIVLPARIAEVATQAVIFDRADGPEAALAMVERRSGGWFDPGIV
                     DAFRRMGPELLAEGAEEDPWEAVLAAEPEPLAMVGAAGLDRVARAFADMVDLKSPYTL
                     GHSTGVAKLAVEAGRSLGLNEDDLSELRLSALMHDVGRTGISNGIWDKPGPLTTAERE
                     MVRMHPYHTERILARSTALAPLVPIAGAHHERQDGSGYHHGASGAEIPVAARILAAAD
                     AYQAMTQERPHRPARPPEEAAEVIAAEASAGRLDPDREVEVLRLVARGLSNKEIAERL
                     FISPRTAEHHVQHIYAKIGSSTRASAAVFAMEHDLLGP"
     gene            10793..11083
                     /locus_tag="VF058_09835"
     CDS             10793..11083
                     /locus_tag="VF058_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208639.1"
                     /translation="MTMFKGTGEEFDEGARNARENVAPALRELEGFAGLLILGDRSSG
                     RSMSVTFWETEDALRSSEEAAEEMRVETAARYDEEILGVETYDLVIDERGAR"
     gene            11080..11538
                     /locus_tag="VF058_09840"
     CDS             11080..11538
                     /locus_tag="VF058_09840"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6208640.1"
                     /translation="MTELRFTVDAPVPAERVIEALTDFSDRRPDLWPELDPDAYRVEE
                     LGVTSALVREGQRKPRLWALEEYDWSTPGTVTWTARESSFCAPGSFMSARVEPLPNGG
                     SRMHVTWSRKGVGLKGRMVVGLMRLTRGQPLAKSLSRALAGLPQATDAGG"
     gene            11648..15550
                     /locus_tag="VF058_09845"
     CDS             11648..15550
                     /locus_tag="VF058_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX6208641.1"
                     /translation="MRSSRALAIGITLSLTAGALLRAPPAASTQPQPPRASSRPAFVP
                     GELLIQFRPGTPAVERAAARARHGLTRVKGLPLPDLELVRFPGQGRPPEVAARVAADV
                     AVRAAEPNFLWYPDQVPNDPEFDELWGLHNDGQTGGEEDADIDAPEGWAVAADSSDLV
                     VAVIDTGIWIDHPDLQGQIWTNPGESGGGKETNGVDDDLNGYVDDVHGWDFFNNDATV
                     FDPNQPDDHGTHVAGTIAATIDNSIHVAGVSKARIMPLKFLGPEGGDTADAIEALDYA
                     IDKGAHLTNNSWGGGSESQMLQQKIEEANAAGILFVAAAGNDGVDTDTGPNAHYPSSY
                     SNANVISVAATTDTDALAPFSNFGDVTVDLGAPGASILSTVPRFAKAVLVGPDTDMGV
                     AYRSAFHTFGLEGITSNAHRQAILSAALTWMGVSSSSPILIVDDDDGATFEVAYSEAL
                     NALGYTNTVTHTVGHNSSGPMATFMDDHDAVIWFTGNEWDFTLTGQDQTRLNSYLSGG
                     AGRNLLLFGQDIGCDLTNDTGCDLTKDPPVTSTFIRDTLRADYVADIDVNFRPSGVDS
                     TPYASVPTIQLNSSTSLGGQFFSSALEARPGAIAGMRGAPTTWFNGTSMATPHVTGAA
                     ALVMATHPALGHLDVKARLLARGDPLPALAGKTVSGRRLNVASALGLPGPPTDVSAQA
                     AAGGGIDLSWEASSGSDVAGYNVYRGTMSGFPPSPANRIASLVAGTEHSDPGWNTVDG
                     TTYYYVVRAESSEGDEGEVSSEVSATADATPPEVTSRTPEDAETEVAVTTSISVTFSE
                     AVEPSTVGTSSFTLTGPSGAVPGSVSLDPSGTEATFTPAVHLEFGSPYTAEVMSDVED
                     PAGNPISPAVTWSFETTATDSVAPDAEIQQPIPGAYVNDQPHEATGAATDDVEVDEVE
                     VAVRWDSNEGPLYWDAGSADFVPGETWNEATIEPAVSPGSVTWSLGWMPPVADVEPFT
                     VLARAIDTSGNATDPPAEAGFSVDNTKPTGSVSIQNGNPFTGTRSVTLTLTASDGSDI
                     EFSVANDAEPDGPWQPFVQSLAWDLADGPDGTRDVHVLFRDAAQNESTVVESIVLDTT
                     GPTTVFDRLPVYQRARAFDVSWTAEDATSGLEALRVDVRETKQRGAWSSWTEWEAASG
                     TSAAFQGKPGRTYCFRLTATDLAGNASGLESACTAVPANERSFERRGSWKRRSAARSY
                     LGTFTESERRGDRLIVGRVKARTIALVHTRCKRCGKVEILFKGKVIRRLSLKAPKVKR
                     LAVRKVATFDHVRAGKLVIRVRSQGRRVPIEGVGVSRV"
     gene            complement(15555..15944)
                     /locus_tag="VF058_09850"
     CDS             complement(15555..15944)
                     /locus_tag="VF058_09850"
                     /inference="COORDINATES: protein motif:HMM:NF040028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6220 domain-containing protein"
                     /protein_id="HEX6208642.1"
                     /translation="MVRSLRIVYAALAWLFLAALILQVFFAGLMLFGEPEGRALHENT
                     GWMLHTAVLLLPVAAALARAGARAIWLTLGLTVATLIQPILTFVRETPAIAALHPVNA
                     ILMTMLSLIVAQGALRLARTERIPAAS"
     gene            complement(15993..16634)
                     /locus_tag="VF058_09855"
     CDS             complement(15993..16634)
                     /locus_tag="VF058_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007454953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6208643.1"
                     /translation="MGKVRFQLAISLDGYVAGPDQSEEHPLGVGGMKLHEWVFELEAW
                     RRQQGMDGGVVNASTQVVEEAQTGVGATVMGRNMFGGGPGPWPPDPPWDGWWGEEPPF
                     HGPVFVLTHHPRESLEMRGGTTFTFVTDGIEAAMEQARAVAGDQDVMIGGGANVIQQY
                     LSAGMVDEFELHLVPIILGAGKRLFENVGRCDLEQVRVIEAPGVTHLKYRVRR"
     gene            16709..17152
                     /locus_tag="VF058_09860"
     CDS             16709..17152
                     /locus_tag="VF058_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013476057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEX6208644.1"
                     /translation="MGNSYVKLGIALFLSLIVMFVLTMSMIVTIDHFYFNWSNLWMAI
                     IMVAPMGIIMLAVMGGMFENGRLNLALFAAFAGIFAGAFFLGRAETFVGDEGFLRSMI
                     PHHSRAILVCEEASITDPEIIELCDQIVETQREEIRQMEEILERY"
     gene            complement(17149..17730)
                     /locus_tag="VF058_09865"
     CDS             complement(17149..17730)
                     /locus_tag="VF058_09865"
                     /inference="COORDINATES: protein motif:HMM:NF042672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6624 domain-containing protein"
                     /protein_id="HEX6208645.1"
                     /translation="MGRRHAARGICLLAIALASCSSGEIQRDPELRRELLEMRDADQA
                     ERTGQAPGGVETDRARAERLKEIIDEHGWPTIDLVGRDGASAAWLIAQHADFDVALQE
                     RALELMREALDDDQVDPVDVAYLEDRVAVNRGRPQTYGTQIRCMDGRPQPATPIRGEA
                     GVDARRKEVGLDPLEDYYAELEEACAAEAAGMP"
     gene            18035..18208
                     /locus_tag="VF058_09870"
     CDS             18035..18208
                     /locus_tag="VF058_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208646.1"
                     /translation="MDDLASPFEEQRPHLRAVAYRLLGSVDDADDALRRVTAIEMLAD
                     AERLRDLDLEPIG"
     gene            18205..18651
                     /locus_tag="VF058_09875"
     CDS             18205..18651
                     /locus_tag="VF058_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6208647.1"
                     /translation="MTEVPWGRFAEVEPELAAFGAERLARPPAYLATVRSDGRPRVHP
                     VTPILPADALFVFMEPTSPKGRDLAKRGSFALHNGVPDTAGTGGEFWIGGRGFLVEDP
                     KIRAAAKEAAAYDPVDRYVLFELRISEARCNAYGDVRLPARRRWSV"
     gene            18775..19740
                     /locus_tag="VF058_09880"
     CDS             18775..19740
                     /locus_tag="VF058_09880"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX6208648.1"
                     /translation="MGHRGGRGIHQPRRVRARRRRALSVAVGVLILIPTGTSARQPST
                     CGGESVTILGTQADDLLVGTPGTDVIAAGSGDDVVLGLGGADRICGGRGDDRLRGGGG
                     RDLLFGGPGDDVARGGPGGDGLQGGRGRDRLLGGRGADVARGGPGGDRIRGGPGRDTA
                     VGGLIGGGGPDTVHGGRGSDRLTDGPGKDRLLGGRGPDMLIPSVHPEEDLLDGQAGRD
                     LVFYFHVPRGVRVHLALGITEGYGNDRLRRIEDAEGGRFDDVLIGDAGPNELLGAEGD
                     DRILGRGGPDLLDGFAGTDDLRGGPGRDRCIDGERHDSCEVIWPS"
     gene            complement(19760..20119)
                     /locus_tag="VF058_09885"
     CDS             complement(19760..20119)
                     /locus_tag="VF058_09885"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6208649.1"
                     /translation="MRPQPDPVVHIELRTNDVERARIFLGRLFGWNSERIRVGGRQYL
                     ALDVGDRVEGGIAARDGDPPAWLPYVEVADVDEAAERARALGASVVLPPTEGPAGWHA
                     ILGMPAGGEVGLWQPKR"
     gene            20206..21231
                     /locus_tag="VF058_09890"
     CDS             20206..21231
                     /locus_tag="VF058_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6208650.1"
                     /translation="MPATHADESETLALARAGDEEAFRLLVEPHRPKLHAHCYRMLGS
                     LHDAEDVLQDALLRAWRGLPGFDGRSAFGTWMYRITTNACLDAIAKRPKRVLPVDYGP
                     PASNGSEVGTRPAGVAWLEPYPDDAYAIEDPGPDARYEQRESIELAFVAALQHLAPRQ
                     RAVLILREVLGFSAKEVAEVLGSTPASVNSALQRARKAVQERIPARSQQATVRSLGDE
                     RVREIVERFTDAFDRGDTGAILSMLAEDATFSMPPYAGWVRGREAVGRSWLIPEGPSP
                     RLRYLPTRANGQPAVAAYALAPDGTRYVPLALDVLTLRDDLIGDVTAFRSPELFEAFG
                     LPPELAA"
     gene            complement(21272..22339)
                     /locus_tag="VF058_09895"
     CDS             complement(21272..22339)
                     /locus_tag="VF058_09895"
                     /inference="COORDINATES: protein motif:HMM:NF021599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
                     /protein_id="HEX6208651.1"
                     /translation="MIVEGGRRTLAERCRVQAGWCGRLGSPLYADLLRRAADDAEAGG
                     PVWRALRGREDAPEEDYVALRLMGSVHRLVLQGRAPALAAHYPSVGGRPGKRAWEDFR
                     DVVDAHGDDLRGLLDLPPQTNEVGRSAALLGGFLLVARESGLSLRLLELGASAGLNLR
                     WDRYRYESGEWGWGDPASPVRFEDVFESATPKGRDVVVGERRGCDLAPIDPTTEDGRL
                     TLLSYVWPDQTERIERLRGALRVAAEVPARVERADAASWLGKELGRQRRGEATDVFHS
                     VVLGYLDEGSRRELAGILEEAGAAATAEAPMAWLALERAGDVFEVRLRSWPGGHDRLL
                     ALTEPHGPPVRWLESDDQSAT"
     gene            complement(22341..22778)
                     /locus_tag="VF058_09900"
     CDS             complement(22341..22778)
                     /locus_tag="VF058_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018777848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX6208652.1"
                     /translation="MTEDVRTEQSIGIDAPIDEVWAALTTPDQIEQWFLGVRTETDWR
                     PGSPIVHRGEYQGKPYEDVGTILEFEPPRRLVHTHWSPVSGRPNVPESHEVVVWELAE
                     RGAGTVLTVEESNLASEEAKATSERTWGVVLENLKGFLESRAS"
     gene            complement(22809..23384)
                     /locus_tag="VF058_09905"
     CDS             complement(22809..23384)
                     /locus_tag="VF058_09905"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEX6208653.1"
                     /translation="MAATSSDPGRVAEPPSRPLETAITLVLEDQSPELARAHRELYPE
                     RIPEQIPLSLTLLYPWIPAAEVTDGDVERLRSFFADRPPLEFALTRVAAFPGLVVYAV
                     PDPDEDLRATMRALWARYPQHPPYGEEGSDPPPHCTLGRLEGDHAVTLEEVASRVEPL
                     LPISCTVRAATLLEEYEVDRFRTRTVLPFAG"
     gene            complement(23351..23953)
                     /locus_tag="VF058_09910"
     CDS             complement(23351..23953)
                     /locus_tag="VF058_09910"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX6208654.1"
                     /translation="MRGARRRRDERELVALPPTLPIPEDDGGARHLPGVRVPDVALAS
                     TEGGSMGLADLDSSRTVVYCYPRTGRPGEPPLREDWDRIPGARGCTAESCAFRDRHAE
                     IASLGVGVYGVSTQAPEDQREAARRLRLRFPLLSDAGLELVRALRLPTFEVAGRTLLK
                     RLTLIVRDGRIEHVWYPVFPPDRHAEEVLGWLRRHPIRAA"
     gene            complement(24084..>24960)
                     /locus_tag="VF058_09915"
     CDS             complement(24084..>24960)
                     /locus_tag="VF058_09915"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6208655.1"
                     /translation="DMGLEPSVVPKMGLAEKCRHCGRCVFGCPYDIKWDTRAFLDQAV
                     ARGAELVTGARVDRLMIEGGRARGVLVGRGPGRRLYEADLIVIAAGGFGTPAILERSN
                     LACEPGLFVDPVLTIAARIPAAWQCNEIEMPFVVQRPHYLLSPYFDWISALFNPAWRF
                     PVPDMVGVMIKLADEGTGSVSLRGRVDKRLTAVDRSRLEEAAGIATEILGRFGADPES
                     VFTGTVNAGHPGGALPLSEATVTSFHDERLPENVYVADASLLPRSLGGPPILTIIAMA
                     KRVARLCRDRAFAGV"
BASE COUNT         3868 a         9111 c         8516 g         3465 t
ORIGIN      
        1 gtcaccccca gatcgaagga cgggacaagc ctagagcaat gcgtgcgctc agtgctcggc
       61 cgcgtcggcc tcttccttgg tccggtggac ggtccccgcc gggtgctccg ggggcgcgta
      121 gaccgtcacc acacggagcg gctcgtctcc cacgttgcgg aagttgtgcc gcgtccccgc
      181 gcggacgaag accatgtcgt tcgggcccac gggtccggtc tggccgtcga gcacggcctc
      241 tccccgcccg gcgacgaaga gcaggatctg gtccacgtcg tcgtggacct cctccccgat
      301 ctccccgccc gcggcgatcg tcatgaggac gacctggccg tgctcaccgg tgatcacctc
      361 tcgacggaag tcctcgttct cggatgccag ccgcatgatg tccatccccg ccgccatggc
      421 gcctcctctc actgctcgct tccctaccct tgccgtggag gggtcatgcg ggcccgcgcc
      481 cctcgaccag ctcgtgccgg agggtcgtgc ggtcgaggcc ctccttcctc acccggaggt
      541 cgaggtacag gaggacgacg accgcgacca cgtagggcgc cgtcaggacc gatgcgatcg
      601 acgcggccag gccctgcgcg aaccagtgat cgccgacggg acgcgtgacg atgcccgtca
      661 ggatgcccgt gacgaggagg acgacgagca tcgccccgaa cacgggccag gagcggcccc
      721 tcaccagctc ccacgatctc gagatcgccc gccccccggt cgcccgctcg accaccaccg
      781 cagggaccgt ggcggtcagc cgaacgagca cgatgaggcc cgggatgatg aacaggacga
      841 acccggctcc caccgcgagg cccatcagga gcgtcgcaag aagaagcgac cctagcctcc
      901 cgagagccgc gcggtagctc agcccgacgc cgggctcgtc cccggccacc gccgagacga
      961 cggcgtgcgt cccggcgccg gtggcgagct gggagaccac gaagctgatg aggcccagcg
     1021 cgagccctcc caggctgacc ctccaggcct cggaggcgtc gagggtccga accccgcccg
     1081 gggcggcccc ccagacgtcc gcgaccccga cgaacaggag gtactgcagc agcgtgaggg
     1141 ggacgacgac caccgccgcg atcgacacca gcacgaggaa gttcctccgg tagacctcga
     1201 acgcgtccgt caggagctcc cccaccgtcc gggcccggag ctccaggcct cgctcgccca
     1261 catcgcacct ccctcggcgt cacccgctcc tcgggtcatc ccacacttcc cggggcgagg
     1321 caacctcacg cgtggaaggc tgcgagccgc tccgcctcct cctcgacctc caccatggtc
     1381 gctcgcgaga ggcgctcgaa cggctcaagc cgcacgcggc cgccctcgta ccgccaggcg
     1441 ccggccacag acccgtccac gaggaagggg gtaccgattg ggggttcttc gtatggaaga
     1501 tccgggggtc gagagcacgc actcgctcat tcggccgagc ctaacgggtg gcgacgccct
     1561 cagggtcgag ctcctcgcgg atcagccgga gctcctcccc ggttgggggc ggggtctcgg
     1621 agagatcctc cgagaccctc agctcccagc cggtctccgc ccggacccga tcgagcggga
     1681 cgcccgggtg gaggctccgc agctccatct cgcccgaggc gtcgaacccg tgcacaccca
     1741 ggtcggtcac gacgacggtg ggcccggccc cgatccagcc ttccggcctc ctccccgcaa
     1801 ggtgtccggg gctggtcgtg aagtcgaccc gctccacgaa cgagcgccgg gactgcgacg
     1861 tgatcacgaa gaccttccgg gcgttgatcg cgatctcgca ggcgccgccg gagccgggca
     1921 gacgcacctt cggggccccg tactccccga tcacggtcgt gttgatgttg ccgtggcggt
     1981 cgatctgaga ggcacccagg aacccgacgt cgaccagccc gccctggagg tagaaggcga
     2041 acagctcgaa catcccgtgg atcgcgtcgg agcccgtggc cagcgaggga tcgccgatcg
     2101 acagagggag cctggccggg cgcgccccga acacgccggc ctcgtagacg agctggaggc
     2161 ccgggctgac cgttcgccgg gccaggttgc aggcgatgtt gggcggcccg acgcccacga
     2221 agcacacccg ttcgccggcg agctgccggg cgctggccac gaccatcatc tcggtgggcg
     2281 tccagccggt cactcgtagc tcccgtagtt cacgcggccc gaccaggcct cccccggcgc
     2341 cagctcggcc caccgctcct cccccagctt ctccgcgtac tcctcgtgcg acgagacgcc
     2401 ccgcacccac tcgtcgagcc aggcctcgag gcgctcgggg tcgcgcgaga tcgcgtccca
     2461 ctcccggtag aagcggttgt cgcggtcgta gtagccctgg gcgaacgacg ggtggcatcc
     2521 tcggggcgcg acgaccaccg cgtcgacgat gaggccgggg atcaccgtgc ggttcgggtc
     2581 ggcccggatc acctcgggct cgacgagctc ctcgcagacc acgaggaccc gttccccggc
     2641 gaacgccgcc tcccgctgga ccccgaggag gccccagatc tgcgcgttgc cctcggcgtc
     2701 ggcccgctgg gcgtgcacca cgaccacgtc ggggttgagc gggggcacgg cgtagaggcg
     2761 ctcgcccggg tcgtaggggc tctcgatcgg cacgatccgg gggttgacct tcggaaggtc
     2821 gctcccttcg tagctcctga gggggaagaa ggggagccga gacgccccgg ccacgtagcg
     2881 gcacaccatc ccgaagtgcg agtactcctc gagctcgagc ggatctccct ctcgaccctc
     2941 cacccgccgc cgcaccgcgt ggagggaccc cacgcccggg ttccccagcc aggagaagac
     3001 gagccgcctc gcgcacccgg ccgccaccat ctggtcgtag atcaggtcgg gcgtgaggcg
     3061 acacagcgtg aggtcgcggc gcccctggcg gatgatctcg tgccccgcgg cgaacgagat
     3121 caggtgcgtg aacccctcga tcgccacggt gtcgccgtcg cggacgagct cccgaaccgc
     3181 gtcgggcatc gaggtcacct tcgacacgag cggacgctac accatcggcc gtgggtcgct
     3241 atggggcggg cggggcggga ggcgggggtg ggggaacctc gccctcctcc agcgcgggcg
     3301 gacccccgac ggtcccgcgg gccaccagga tcgccgcgcc gagcagcaag agccccacga
     3361 cgagcacggc gatggccggg ccctcgtcct cgacgtggtt ggcgacgatc gtggacgcca
     3421 cgatcagggt gcccgcgatc cccagaccgg cgacgccccg atcctcgatc caggacccga
     3481 ggaccatcaa cgcgaaggcg gtggccagcg ccaggatctc gcccacgatc tcctcgcccc
     3541 cgaccaggaa gaagaagggg ccgatcagcg cgaggatcga gccgaccgcg agcgaggtcc
     3601 tgcgcggacc cagccagccg agccagccca ggaggatcac ggcccctccg aggagccacg
     3661 tcacgaagcc gaccgcgctg aggtcgggcg gcccgaggat gaagtctccg acatccggga
     3721 acacgagcga gatgatcgcg atgtagccgg tgaggatcag caccaggacc tgcaggctcc
     3781 gacgcagcac ccaccacagc acgagggaga agagggccgc caccagggcg gtgaggaaca
     3841 ccaccatccg ctcgtcgagg ccctcgatca cctcgccgaa cagcagcccg gccagatcgg
     3901 ccaccgagaa cacggccacg aaccagaaga cgctctgcat ccgggggtag ggctcgggct
     3961 tcccggcgac cgcccagccc gcggccagca gcaccacgat cgtgatcagg tcgaaggcga
     4021 tgcgaacgcc ctcgcctgcc tcctgaccga gcaccgtcga ggccgcgctc agcgccaccg
     4081 cggcccccgc gtacccgacg aactcgatga ccgtccccag cgagatcggc tcgcgcctca
     4141 gctccatgtc ttcccccctc tcgatgatcg aaaggatgac atggcgaggc gggcctacca
     4201 agcacggtga cgcgcgcgga ggcccgggcc tccgcgcgcg agtcctcgat cccctagctc
     4261 agcgcgtggc ggccttccgg aactggcgga tcgagagcgg gacgaagatc aacaggatga
     4321 tcccgaccca gatcaggccg tagagcaccg ggttctggag ggaccaggct tccggcgtcg
     4381 ggacgttggt cgccgtgttc ccgaacgcct cccgcaccgc ctgcgccacg gccgagaccg
     4441 ggttccactc cgcgaacacg cggagggggc cgggaaggct ctcgctcagg acgaacgtgt
     4501 tggccacgaa cgtcaccggc atgatgaaca tgaacgaggc gttctggaag acctccaggc
     4561 tcgacacctt gaggccgacg aacgccatga tccacgagac cgcgtaggcg aacagcagga
     4621 gcagaaggaa gccaccgatc gccggcagga ggctcgtccg gatcctccac cccacgatga
     4681 ggcccgcgat cgacatcacc gcgatcgaga tcgagttgta caggatgtcg ctcgtcgttc
     4741 gcccgacgag cacggccgac cgcgacatag ggagcgaccg gaaccggtcg atgatcccct
     4801 tggtgatgtc gtcggcgagg cccgcgccgg tgaacgtgga gccgaagatc accgtctgga
     4861 cgaacacccc cgcgatcagg aactcgcgat agctcacgtt gcccgtgtcg atcgacccgc
     4921 cgaacacgta cgcgaacagc aggacgaaca tgatcggcga catcgtcgtc cagatcagga
     4981 tctcggggac ccgcttgatc ttgatcaggt tgcgcttggc gacgacgagg ccgtcgccca
     5041 gcaccatcgc cagcgtgctc atcgggccac ctccttctcc tgctcctgga tccgctcggc
     5101 gtccggcatc tggccgtcgc cgttgccgcc gtcggccgcg ccggccgagt gcccggtcag
     5161 cgtcaggaac acgtcgtcca gcgtgggacg acgaagcccg atgtcgtcga tctgcaccga
     5221 ccgctgctgg aggaggtcga gcacccgtcg aaggtcctca gacccgcctc gcaccgcggt
     5281 ggtcaagctc ctcgtgtgct ccgtgacctg cacctcgtcg gtgcagacgg tctccaggat
     5341 cgagcgggcg ctctccaggt cggccatgtc gccgagcacg agctcgagcc gctccccgcc
     5401 gacctgcagc ttgagctggt cggccgtgcc gtgggcgatc ttctggccac ggtcgatcac
     5461 gacgatatcg tcggccaggc ggtcggcctc ctccatgtac tgcgtggtga ggagcacggt
     5521 cgatcccccc tgcgcgaggt tctcgatcgt gtcccacatc tggacgcggc tccgaggatc
     5581 gaggcccgtc gtcggctcgt cgaggaagag cacctcgggc tccgccacga gcgcggcggc
     5641 caggtcgagc cgtcggcgca tcccacccga gtacgtcttg gccgggcggt tgccggcctc
     5701 ctccaggtcg aactgctcga ggagctccct ggccctggct cgggacctgg ggccgcccat
     5761 gtggtacagc cgtcccacca tctccaggtt ctcgtagccg gtcaggtact cgtccaccgc
     5821 ggccgtctgc cccgacagcc cgatcacccg ccggacctcg gtggcctggg tgcggacgtc
     5881 gaggcccgcc acctcggccg tcccctcgtc ggcgtcgagg agcgtggtga ggatccgcac
     5941 ggcggtagtc ttccccgctc cgttcggccc gaggaggccg agaacggtcc ctttggggac
     6001 ctccagatcc agcccgtcga gggccgtgac cttcccgtac cgcttcacca aactcgatgt
     6061 cctgatcatc cgtctccttc cgcaaaacag cgccggccgt cgtgccacaa cgtgacccgc
     6121 tcggcagatg ttcccggccg cgcgatcttc agctcgccgc tcgctcccca agcgtcatca
     6181 gatactcgtc caggcgctcg tacgtctccg cggcgcccgt ctccatgccc gactcgatca
     6241 tcgcgtctcg ggcctccttc gagtcgtaca cggcggtggc cgtcacggtg gtcttgccgt
     6301 cgtgctcctc gagggtaagg gtctccaccg acacgctccc cggcgccccg tcgaactcga
     6361 acgtccacgt gatccgctcg ggccgcacga tctcgcggta ctcgccgctg aacccgtact
     6421 cctccccgtc ggggccccgg tggacgatcc gccacgtccc tccctcccgg aagtcgacct
     6481 cgcactccga gatctccatc gccctcgggc cccaccagtt gcgcatgtgc tcgcatgagg
     6541 tatgggcctc gaacacgagg tcccgcggcg cgtcgaacac ccgggtcatc acgatctcac
     6601 gatcagtggg ggtcgagacc tgcagctggt tccccatctt cctcctcctt ccgtcgcccc
     6661 tggagctcgt ccaggtactc gtcgagccgg tcgtagctct cctcccagaa ccgccgatac
     6721 cgatccagcc aggcggcggc ctccctcaac ggctcggcgg ccagacgcgc cggcctccac
     6781 tgccgctccc tgctgcgggc gatcagcccg gcccgctcca acaccttcag gtgcttggag
     6841 acggccggca gggtcatgtc gaacggagcc gccagctgcg tgaccgaggc ctccccctcc
     6901 gacagacggc tcaagatcgc ccgccgcgtg gggtcggcca gggcggagaa cgtcgcgtcc
     6961 agcctctctc tcgtcgcggt actcatgaca ccccccggtt aaataaccga agggtaaact
     7021 acatcgcctt ccgatccgac gtcaagtaca gactcccggg gcgccgtcga accatcggtg
     7081 ggaagtaaga aatgtctccg gctcgctcag cgtcgcgacg ggaccggctg ctcggtcggg
     7141 ccggtgatcc ctgagtcagc cttcccgctt cccgggagcg agcggaggga ccttgcggcc
     7201 ggcggccgag tcctcgatca gtcgctccag ccggcgctcc cgtgtctcct gccgcttcgc
     7261 gctcatcacc cagtggagcg cggacttccg gtacgagggg gccatggcct ggaaccgctc
     7321 ccaggccttc gagctgcggc ggaaccgccg ctccaggtcc tcgtcgagcc tgatcgagtc
     7381 gcgtcgctcg taggagtagg gggaccgatc ctccctccgg gcccggaacg cggcgagccc
     7441 cgccggcgcc atcaggcctc gatcgatcag cgccctggcc cgctcgacgt tccgtgcgct
     7501 ccacaggctc cccggcctcc gaggggtgaa ccgattcacg taccgcttct cgtcgacgct
     7561 tctgcgcacc ccgtcgatcc acccgaagca cagcgccacg tccaccgact cctcccacgt
     7621 catgctgggg acgcccacct gcttcttgtg gaagcccacc caaagctccg tggccgtggc
     7681 gtggtgcgcc tcgagccaag cccggaaccc atccgcatcg gcgaagtaga tcgcatccat
     7741 cggccgccga atctacagtg accgctcgag aagagaaacc tggacgggtc cgggggcggg
     7801 tgttagggtc cgagcgatcc gagcacccgg ggtccccgaa atgggaacga cgagctcaag
     7861 agctccgatg gccgccgcgc tggcggtcgc cctgtgtctt gcgacgcctc tcacggccac
     7921 ggccgccgtc aggatcaggc cggtgaagga catcaagccc gggcccggca gctccctcaa
     7981 cgacgagggc atcgacttca ggcgcgtcgg acgctggctc ctcttcaggg ccgacgacgg
     8041 ggtcgccggg agcgagctct ggcgaacgga cgggacgccc aaggggacga gacgggtgaa
     8101 ggacatcaac cccggccccg ggcactcgag ccccgcggag ttcgagctga tgggtggggt
     8161 cctcttcttc caggcggacg atggagtcac cggtaccgag ctgtggcgga ccgacggcac
     8221 gaggaagggg acgaggcgag tgaaggacat caacccgggg ccggccgact cctctcctca
     8281 agaactgacg cggctcggga agcggatcct cttcaccgcc aacgacggcg ccgccggctg
     8341 ggagctttgg cggactgacg gcacgaagaa gggaacgaga cgggtgaagg acatcaaccc
     8401 ggggccgtcc ttcgcgcagc cgcacgactt cgttcgcctg gggaagaagc tcctcttctc
     8461 ggcatcggac gggaccctga gcatcgagct ctggcggacc gacggcacga agaaggggac
     8521 gcgcctggtg aaggacatcc ggcccggccc gggggactcc tcacccggcg acctcgtgcg
     8581 gctggggaaa cgcgtcatct tctccgccac ccacgacgcc acggggcggg agctctggcg
     8641 gaccgacggc acacggaagc ggacacgcct ggtcaaagat ctctttcccg gaggcaacgc
     8701 cttcccggaa cgactcaccg tgtacgggaa ggtgatcctc ttccgagccg acggcccggc
     8761 ggggcgggag ctctggcgga ccgacggcac gcggaaaggc acgaggcggg tgaaggacat
     8821 caacccggga cccggcgact cggaggtcga cgacttggtc aggttcggca agatcgtgct
     8881 gttccaggcg gacgatgggg tcaccggcac cgagctatgg cggaccgacg gcacgaagaa
     8941 gagaacgaaa cgcctgaagg acatccaccc ggggcccggc tcgtccatcc ccgacaagtt
     9001 cacgaccgtc ggggactggg ccgtgttcaa ggccgacgac ggcaccaccg gagcagagct
     9061 ctggcgcacc gacggcacga agaagggcac caggcgcctc agggacctca accccggccc
     9121 gggagccgcc tatccgggct acttcacgcc cctgggcaga tggttcctgt tcggcgccta
     9181 cgacggggtg acgggctacg agctctggaa gctgaggcgc tagtctgcgg gccagcgtga
     9241 ccgatcagct gcggctcgcc gatctgctcg tggccatctc ggtcgccacc gacctcggga
     9301 tgggtcagga gcccgagaag gccgcgcgct cctgcctcct ggcgacccgg atggcccggg
     9361 ggctcggcct gcccgaggcc gaggtgcgcg aggtctacct ggccaccctc ctgcgccacc
     9421 tcggatgcac ggccacggcc cacgaagagg cccacctgtt cgggggggac gagctcgcgt
     9481 cccggccgcc gtccgaggcc gccgacttcg gcgaggcccg ggagatgctg aagctcacac
     9541 tggccacggg gcggggggcg gggcttcgcc ggccccggct cctggcgcgg gccctcctcg
     9601 ccggccggaa ggagagcgag gggatcctca gggcgatctg cgaggtcggc tcccacctgg
     9661 cctcccgtct cgggctgggc cacgcggtgg agcgcgcgct gtaccaggtc acggagcgct
     9721 gggacggcac gggcacgccc gagggcgccg cgggcgagga gatcgtgctg ccggcgagga
     9781 tcgccgaggt cgcgacgcag gccgtgatct tcgatcgggc cgacggcccc gaggcggcgc
     9841 tggccatggt cgagcggcgg tcgggtggct ggttcgaccc ggggatcgtt gacgcgttcc
     9901 ggcggatggg acccgagctc ctggccgagg gggccgagga ggacccttgg gaggcggtgc
     9961 tcgcggccga gcccgagccc ctggcgatgg tcggggcggc ggggctggac cgcgtggctc
    10021 gggccttcgc cgacatggtg gacctgaagt ccccgtacac cctggggcac tccaccggcg
    10081 tcgcgaagct cgcggtcgag gccggacgat cgctcggcct gaacgaggac gacctgagcg
    10141 agcttcgcct ctcggccctg atgcacgacg tgggtcgcac cgggatctcg aacgggatct
    10201 gggacaagcc gggcccgctc acgaccgccg agcgggagat ggtccgcatg cacccgtacc
    10261 acaccgagcg gatcctggcc cggtcgacgg ccctcgcccc tctcgtgccg atcgccggcg
    10321 cccaccacga gcgacaggac ggcagcggct accaccacgg cgcctccggc gccgagatcc
    10381 ccgtcgcggc ccggatcctc gccgccgccg acgcctacca ggcgatgacc caggagcgtc
    10441 cccaccgacc ggcccggccg ccggaggagg ccgcggaggt gatcgcggcc gaggcctccg
    10501 cgggacgcct cgaccccgac cgcgaggtcg aggtcctccg cctggtcgcc cgggggctgt
    10561 cgaacaagga gatcgcggag cggctcttca tctccccccg cacggccgag caccacgtcc
    10621 agcacatcta cgcgaagatc ggcagctcca cccgcgcttc cgcggcggtc ttcgccatgg
    10681 agcacgacct cctgggcccg tagcccggcg gaatgggcag tcctaccgat gcgggcgcgc
    10741 cggcccgagc gccatcctcc tcacgaagga ggtggtgtcg atgttcgcac gggtgaccat
    10801 gttcaagggc acaggcgagg agttcgacga gggcgcgagg aacgcccggg agaacgtcgc
    10861 tccggcgctc cgcgagctcg aggggttcgc cggcctgctg atcctgggtg atcgctccag
    10921 cgggaggtcg atgtcggtga ccttctggga aacggaggat gccctccgct ccagcgagga
    10981 ggccgccgag gagatgcgcg tcgagaccgc ggccaggtac gacgaagaga tcctgggcgt
    11041 cgagacctac gacctcgtga tcgacgagcg gggtgcgcga tgaccgagct ccgcttcacc
    11101 gtggacgctc cggtcccggc cgagcgggtg atcgaggccc tgaccgactt ctcggatcgc
    11161 cgcccggacc tctggcccga gctggatccc gacgcttacc gggtggagga gctcggggtc
    11221 acgtcggccc tggtccgcga gggccagcgg aagccccggc tgtgggcgct cgaggagtac
    11281 gactggtcca cgcccggcac cgtgacctgg accgcgcgcg agagcagctt ctgcgccccc
    11341 ggcagcttca tgtcggcgag ggtcgagccc ctgccgaacg ggggcagccg gatgcacgtc
    11401 acgtggagcc gcaagggcgt ggggctcaag gggcggatgg tcgtcggcct catgcgcctc
    11461 acgcgaggcc agcccctggc caagagcctg tcgagggcac tggccggcct gccccaggcg
    11521 accgacgccg ggggctgagc tcgtaccggt caagcgtgct cctttggggg ccgatatgaa
    11581 ggtcgggctc gataagcgtc ctcgccggta tgctggtcga gcccggaccc gaagcccccc
    11641 gaggtacatg cgatcctcca gagccctcgc aatcggcatc accctcagcc tcacggccgg
    11701 agccctgctc cgggcgccgc ccgccgcctc gacccaaccg cagcccccgc gggcgtcgtc
    11761 gcgaccggcg ttcgtccccg gggagctgct gatccagttc cgaccgggga cgccggccgt
    11821 cgagcgggcc gcggcacggg cccgacacgg cctgacccgg gtcaaggggc tgcccctccc
    11881 cgacctcgag ctcgtgcggt tcccggggca gggccggccc cctgaggtcg cggcccgggt
    11941 cgcggcggac gtcgccgtcc gagccgctga gccgaacttc ctctggtacc cggatcaggt
    12001 accgaacgac ccggagttcg acgagctctg ggggctccac aacgacggcc agacgggcgg
    12061 ggaggaggac gccgacatcg acgccccgga gggctgggcc gtggccgccg actcgtccga
    12121 cctcgtcgtg gccgtgatcg acacggggat ctggatcgac caccccgacc tccagggcca
    12181 gatctggacc aacccgggcg agagcggcgg cggcaaggag accaacggcg tggacgacga
    12241 cttgaacggc tacgtcgacg acgtccacgg ctgggacttc ttcaacaacg acgcgaccgt
    12301 gttcgacccg aaccaacccg acgatcacgg gacgcacgtg gcggggacga tcgcggcgac
    12361 gatcgacaac tcgatccacg tggcgggggt gagcaaggcg aggatcatgc ccctcaagtt
    12421 cctggggcct gaggggggcg acacggcgga cgccatcgag gcgctcgact acgcgatcga
    12481 caagggcgcg cacctcacga acaactcctg ggggggcgga agcgagagcc agatgctcca
    12541 gcagaagatc gaggaggcga acgccgcggg catcctcttc gtcgcggccg ccggcaacga
    12601 cggcgtggat accgacaccg gaccgaacgc tcactacccc tccagctact cgaacgcgaa
    12661 cgtcatctcg gtagccgcga cgaccgatac cgacgcgctg gcccccttct cgaacttcgg
    12721 ggacgtcacc gtggatctgg gcgctccggg tgcgtcgatc ctctccacgg tcccgcgctt
    12781 cgccaaggcg gtcctcgtgg ggcccgacac cgacatgggg gtcgcctacc ggtccgcctt
    12841 ccacacgttc gggctggagg ggatcacctc caacgcgcac cgtcaagcga tcctctcggc
    12901 ggccctgaca tggatgggcg tctcctcgtc gagccccatc ctgatcgtgg acgacgacga
    12961 cggggcgact ttcgaggtcg cgtactccga ggccctgaac gcgctgggct acaccaacac
    13021 ggtcacccac accgtggggc acaacagtag cggtccgatg gcgacgttca tggacgacca
    13081 tgacgcggtc atctggttca cgggcaacga gtgggatttc acactgaccg gtcaggatca
    13141 gacccgtctg aatagctacc tctcgggcgg ggccggccgg aacctcctcc tgttcggaca
    13201 ggacatcggg tgcgacctca cgaacgacac cgggtgcgac ctcacgaaag acccaccagt
    13261 gaccagcacg ttcatccgag acacgctccg cgccgactac gtggcggaca tcgacgtgaa
    13321 cttccggccg tcgggggtag acagcacacc ctacgcttcc gtccccacga tccagctcaa
    13381 ctcctccacc agcctgggcg ggcagttctt ctcgagtgcg ctggaggccc ggccgggagc
    13441 catcgcgggg atgcgaggcg cgcccacgac ctggttcaac gggacctcga tggcgacccc
    13501 acacgtcacg ggagcggccg ccctggtcat ggcgacgcat cccgccctcg gccacctgga
    13561 cgtgaaagcc cggctcctcg ctcgagggga ccccctgccc gccctggcgg ggaagaccgt
    13621 ctctgggagg cggctcaacg tggcctcggc gctcggcctt ccgggaccgc ccacggacgt
    13681 gtcggctcag gcggccgccg gcggagggat cgacctctca tgggaggctt cctctggctc
    13741 ggacgtggcc ggctacaacg tgtaccgggg tacgatgagc gggttcccgc cctcacctgc
    13801 caaccgcatc gcctcgctcg tggccggcac cgagcactcg gatccgggct ggaacaccgt
    13861 cgacggcacg acctactact acgtcgttcg cgccgagagc tccgagggcg acgagggcga
    13921 ggtctcgagc gaggtctcgg cgaccgcgga cgccacccct cccgaggtga cctccaggac
    13981 ccccgaggac gcggagacag aggtcgccgt gaccaccagc atcagcgtca ccttcagcga
    14041 ggcggtggag ccgtcgacgg tcgggacgtc ctcgttcacc ctcaccggcc ccagcggcgc
    14101 cgtcccgggc agcgtgtccc tcgacccctc gggcaccgag gcgaccttca ccccggccgt
    14161 ccacctcgag ttcgggtctc cttacaccgc ggaggtgatg agcgacgtcg aggacccggc
    14221 cggcaacccg atctcgcccg ccgtgacgtg gtcgttcgag acgaccgcga cggactccgt
    14281 ggcccccgac gccgagatcc agcagccgat ccccggcgcc tacgtcaacg atcagcccca
    14341 cgaggcgacc ggcgcggcca cggacgacgt cgaggtcgac gaggtggagg tggccgtgcg
    14401 atgggactcg aacgagggcc ccctctactg ggatgcgggc tcggcggact tcgtcccggg
    14461 cgagacctgg aacgaggcca cgatcgagcc agcggtctca ccggggagtg tgacgtggtc
    14521 cctgggctgg atgcctccgg tcgccgacgt cgagccgttc accgtgctgg cccgggcgat
    14581 cgacacctcc ggcaacgcga cggacccgcc ggccgaagcc gggttctcgg tggacaacac
    14641 caagcccacc ggctcggtgt ccatccagaa cggcaacccg ttcaccggca cgagatccgt
    14701 gacgctgacc ctgacggcct ccgacgggtc cgacatcgag ttcagcgtgg cgaacgacgc
    14761 ggagcccgat gggccgtggc aacccttcgt ccagtccctg gcatgggacc tcgcggacgg
    14821 acccgacggg acccgggacg tgcacgtcct gttccgcgac gcggcgcaga acgagagcac
    14881 ggtcgtcgag tccatcgtgc tggacaccac cgggcccacc accgtcttcg atcggctgcc
    14941 ggtctaccag cgggcccgcg ccttcgacgt ctcgtggacc gcagaggacg cgaccagcgg
    15001 cctcgaggct cttcgcgtgg acgtccgcga gacgaagcag aggggcgcgt ggagctcctg
    15061 gaccgagtgg gaggccgcct ccggtacgtc cgccgccttc caggggaagc cgggccgcac
    15121 gtactgcttc cggctgactg ccaccgacct cgccgggaac gcatccggcc tcgagagcgc
    15181 ctgcacggcc gtccccgcca acgagcgatc cttcgagcga aggggctctt ggaagaggcg
    15241 atccgccgcc cgctcctacc tcgggacgtt cacggagtcc gaacggcgcg gcgaccggct
    15301 gatcgtcggg agggtcaagg cccggacgat cgcgctggtc cacacgcgct gcaagaggtg
    15361 cggcaaggtc gagatcctct tcaagggaaa ggtcatccga cgcctcagcc tcaaggctcc
    15421 aaaggtgaag cgcctcgcgg tgcgcaaggt cgccaccttc gaccacgtcc gggcggggaa
    15481 gctggtgatc agggtgcggt cgcaggggcg tcgcgtcccg atcgagggag tgggcgtcag
    15541 ccgggtctga gcggtcagct cgcggcgggg atccgctccg tccgcgccag ccgaagggca
    15601 ccttgagcca cgatcaacga cagcatggtc atcaggatcg cgttgaccgg gtggagcgcg
    15661 gcgatcgccg gcgtctcgcg cacgaaggtg aggatcggct ggatcagcgt ggccaccgtc
    15721 agtcctaggg tgagccagat ggcgcgcgct ccggcccgcg ccagcgcggc ggccaccggc
    15781 aagagcaaca cggccgtgtg cagcatccag cccgtgttct cgtggagggc acgaccctcg
    15841 ggctcgccga agagcatcag ccccgcgaag aagacctgga ggataagcgc ggccaggaac
    15901 agccaggcca gcgccgcgta gacgatgcga agggatctca ccatgtcgcc ggtgaggata
    15961 gccgaagtcg tcggcccgag gcgcagctcc gttcagcggc ggacgcggta cttcaggtgc
    16021 gtgacgccgg gcgcctcgat aactcgcacc tgctcgaggt cgcaacggcc gacgttctcg
    16081 aagagtcgct ttcccgcccc aaggatgatc gggaccaggt ggagctcgaa ctcgtccacc
    16141 atgcccgccg agaggtactg ctggatcacg ttcgcgcccc cgccgatcat cacgtcctga
    16201 tcgccggcga cggctctggc ctgctccatg gccgcctcga tcccgtcggt gacgaaggtg
    16261 aacgtcgtgc cgccgcgcat ctccagcgac tcccgcggat ggtgggtcag cacgaagacg
    16321 gggccgtgga aggggggctc ctctccccac cacccgtccc agggagggtc cggcggccag
    16381 gggccagggc cgccgccgaa catattccgt cccatcacgg tcgcaccgac gcccgtctgc
    16441 gcctcctcga cgacctgggt gctggcgttg acgaccccgc cgtccatccc ctgctgcctc
    16501 cgccacgcct cgagctcgaa tacccactcg tggagcttca tccccccgac cccgagcgga
    16561 tgttcctcgc tctggtcggg gccggcgacg tagccgtcca gcgagatcgc gagctggaac
    16621 ctgacctttc ccatcgtgcg acctcctcgg acgttcggcg gccccatctt cgggtaggga
    16681 ctcgtcactc cacaaccagg aggtaccgat ggggaattcc tacgtgaagc tggggatcgc
    16741 gctgtttctg agcctgatcg tgatgttcgt gctgaccatg tcgatgatcg tcacgatcga
    16801 tcacttctac ttcaactgga gcaacctctg gatggcgatc atcatggtgg ccccgatggg
    16861 gatcatcatg ctggccgtga tgggcggcat gttcgagaac ggccggctca acctcgccct
    16921 cttcgccgcc ttcgcgggga tcttcgcagg cgccttcttc ctcggacgcg cggagacctt
    16981 cgtaggggac gagggattcc tccgctcgat gatcccccac cactcccgcg cgatcctcgt
    17041 gtgcgaggag gcctcgatca ccgacccgga gatcatcgag ctctgcgacc agatcgtgga
    17101 gacgcagcgc gaggagatcc gccagatgga ggagatcctg gagcgctact agggcatccc
    17161 ggccgcctcg gccgcgcagg cctcctcgag ctccgcgtag tagtcctcga ggggatcgag
    17221 gcccacctcc ttccgccgcg catccacgcc cgcctcgcct cggatcggcg tggccggctg
    17281 ggggcgcccg tccatgcatc ggatctgcgt gccgtacgtc tggggccttc cccggttcac
    17341 ggccacccgg tcctccaggt aggccacgtc cacgggatcc acctggtcgt cgtcgagcgc
    17401 ctccctcatc agctcgaggg cccgctcctg gagggccacg tcgaagtcgg cgtgctgggc
    17461 gatcagccac gcggcgctgg cgccgtccct cccgaccaga tcgatcgtgg gccagccgtg
    17521 ctcgtcgatg atctccttca gccgctcggc cctcgcccgg tcggtctcca ccccacccgg
    17581 cgcttgaccc gtccgctcgg cctgatcggc gtcccgcatc tcgagaagct ccctgcggag
    17641 ctcggggtcg cgctggatct cgcccgagga gcaggaggcg agggcgatcg ccagcaggca
    17701 gatccctcgg gcggcatgcc ggcgtcccac ggagcaaggc tagcctctcg ccggttcggc
    17761 gcaccatgcc cgcaacgtcg ctccagcgtc gctcgggcac ggttgacatg tgacctggcg
    17821 ctcacctatc ggttgggcga aggccaccca tcccagcgtc ctgctggatc ggcgccccag
    17881 gccccctcgc cgtaggatcg cttcgccccg gtccacgtcc ctgacgccga gcggaatgaa
    17941 gctcacgtgc gcgtccatac gcctcttcct tccgtcacgg taacctcgtt cgatgcgtca
    18001 tctccatgac ggggcgcgga gcgaggatgt gaccgtggac gacctggcga gcccattcga
    18061 ggagcagcga ccccacctgc gggcggtggc ctatcggttg ctggggtcgg tcgacgacgc
    18121 cgacgacgcc ctccggaggg tcaccgccat cgagatgctc gccgatgccg agcgcctccg
    18181 ggacctcgac ctggagccga tcggatgacc gaggtcccct ggggccgctt cgcggaggtc
    18241 gagcccgagc ttgccgcgtt cggcgccgag cgcctggccc gacctcccgc ctacctcgca
    18301 acggtgcgga gcgacggccg tccgcgggtg catccggtca ccccgatcct gcccgcggat
    18361 gccctcttcg tcttcatgga gccgacctcg cccaagggcc gtgacctggc gaagcgagga
    18421 tcgttcgcac tccacaacgg cgtccccgac accgcgggca cgggcgggga gttctggatc
    18481 ggtgggagag gcttcctcgt cgaggatccg aagatccggg ccgcggcgaa ggaggcggcg
    18541 gcctacgacc ccgtggatcg ctacgtcctc ttcgagctgc ggatctcgga ggcgcgctgc
    18601 aacgcgtacg gtgacgtccg cctcccggcc cgccgccgct ggtccgtctg agcgcggcgg
    18661 cggcgctccg cgacgatcgc ggtcgggctt gccaggatca cggaacggga ggacgatcga
    18721 cccgtcgggg tcagcgcggg ccccgcaggc ccgcgtccga gagcgggcac gccgatgggc
    18781 caccgagggg gacgtgggat ccaccagccg aggcgagtcc gcgcccggcg ccggcgcgcg
    18841 ctgtccgtcg cggtcggcgt cctgatcttg atccccaccg ggacatcggc tcgccagccg
    18901 tcgacctgcg gcggggagag cgtcacgatc ctgggaaccc aggccgatga cctcctggtc
    18961 ggcacgccgg gcaccgacgt catcgcggcg gggtccggag acgacgtcgt ccttggcctc
    19021 gggggcgccg acaggatctg cggcgggcgg ggagacgacc gcctccgggg aggagggggc
    19081 cgcgatctcc tgttcggtgg cccgggcgac gacgtggcac ggggcggccc gggcggcgac
    19141 ggcctccagg gtggtcgcgg ccgcgaccgg ctcctcggtg gtcgcggcgc cgacgtggcg
    19201 cggggcggcc cgggcggcga tcggatccgg ggtgggccgg gcagggacac cgcggtagga
    19261 ggcctgatcg gcggcggcgg gccggacacc gtccacggtg ggcggggcag cgaccgtctc
    19321 accgacgggc cggggaagga tcgcctcctc ggcggccggg gacccgacat gctcatcccc
    19381 tcggtgcacc ccgaggagga cctcctggac ggccaggcgg gccgggacct cgtcttctac
    19441 ttccacgtgc cgcggggagt tcgcgtgcac ctcgccctcg ggatcaccga gggctacggg
    19501 aacgaccggc tgcggaggat cgaggacgcc gaaggcggca ggttcgacga cgtcctgatc
    19561 ggggacgccg gccccaacga gctgctcggg gccgagggcg acgaccgcat cctcggccgg
    19621 gggggacccg atctgctcga cgggttcgcc ggcacggacg atctcagagg tggtcccggc
    19681 cgagatcgat gcatagacgg cgagcgtcac gacagctgtg aggtgatctg gccgagctag
    19741 ggtgccgggc ccgccgggct cagcgcttcg gctgccagag gccgacctcc ccgccggccg
    19801 gcatccccag gatcgcgtgc caccccgcag gcccctccgt cggcggcagc acgacggacg
    19861 cgccgagggc ccgcgctcgc tcggccgcct cgtccacgtc cgccacctcg acgtagggca
    19921 gccacgcggg cgggtccccg tcgcgggccg cgatgccgcc ctccacgcga tcgccgacgt
    19981 cgagcgcgag gtactgccgg ccgccgacac ggatcctctc gctgttccag ccgaagaggc
    20041 gacccaggaa gatcctggcg cgctccacgt cgttcgtccg cagctcgatg tgcaccacgg
    20101 gatcgggctg tggccgcatc atcctcttcg actcgcaccg ggcccccgga ttcatcggga
    20161 gaaccgatga gctagccttc gctcatcggt ccaacggagt agatgatgcc tgcgacgcac
    20221 gcagacgaat cggagacgct ggccctggcc cgcgccgggg acgaggaggc gttccggctc
    20281 ctggtcgagc cgcatcggcc caagctgcac gcccactgct atcggatgct cggctcgctc
    20341 cacgacgccg aggacgtcct ccaggacgcg ctgctccggg cgtggcgagg gctgcccggg
    20401 ttcgatgggc ggagcgcctt tggcacgtgg atgtaccgga tcacgaccaa cgcctgcctc
    20461 gacgccatcg ccaagcggcc caagcgggtg cttcccgtcg actacgggcc ccctgcttcg
    20521 aacggctcgg aggtgggcac caggccggcc ggcgtggcct ggctcgagcc ctaccccgac
    20581 gacgcctacg cgatcgagga ccctggcccc gacgcccgct acgagcagcg cgagtcgatc
    20641 gagctcgcct tcgtggccgc gctccagcac ctggcccccc gccagcgagc cgtgctcatc
    20701 ctccgggagg tgctcggctt ctcggcgaag gaggtcgcgg aggtgctcgg ctcgacgccc
    20761 gcctccgtga acagcgccct ccagcgggcc cggaaggccg tccaggagcg gatcccggcc
    20821 cgaagccagc aggccacggt ccgctcgctc ggagacgagc gggtccggga gatcgtggag
    20881 cggttcaccg acgccttcga tcgaggcgac acgggcgcga tcctctcgat gctggccgag
    20941 gacgccacgt tctcgatgcc gccctacgcc ggctgggtcc ggggacggga ggcggtgggc
    21001 aggtcctggc tcatccccga gggaccgtcg ccgcggctcc gctatctccc cacgcgggcc
    21061 aacggccagc cggcggtggc cgcgtacgcg ctcgccccgg acggcacgag atacgtcccg
    21121 ctggcgctcg acgtcctcac gctgcgggac gacctcatcg gtgacgtcac ggcctttcgg
    21181 agccccgagc ttttcgaggc gttcgggctg cctcccgagc tggccgcgta ggccgagcac
    21241 cgaaccgaag cgcaggcccg gagttcttcg cctacgtcgc cgactgatcg tccgactcca
    21301 gccaccggac gggcggcccg tggggctcgg tgagggcgag caggcgatcg tgcccgccgg
    21361 gccaggagcg cagccggacc tcgaagacgt cgcccgcccg ctcgagcgcg agccaggcca
    21421 tcggggcctc cgcggtcgcc gccgctcccg cctcctcaag gatgccggcc agctcgcggc
    21481 gggatccctc gtccaggtag ccgagcacca ccgagtggaa gacgtccgtc gcctcccccc
    21541 ggcgctgccg accaagctcc ttgcccagcc agctcgccgc gtcggcccgc tcgacccggg
    21601 cggggacctc ggccgcgacc cggagcgcgc cccggagccg ttcgatcctc tccgtctgat
    21661 ccggccagac atacgacagg agcgtcagcc gcccgtcttc ggtcgtgggg tcgatcgggg
    21721 cgagatcgca tccacggcgc tcccccacca cgacatcccg gcccttcggc gtcgcgctct
    21781 cgaacacgtc ctcgaagcga acgggcgagg cggggtcgcc ccacccccac tcgcccgact
    21841 cgtagcggta tcgatcccag cgcaggttga ggccggcgct ggccccgagc tcgaggagcc
    21901 ggagcgacaa gcccgactca cgcgccacga ggaggaaccc tcccagcagc gccgccgacc
    21961 gaccgacctc gttcgtctgt ggagggagat cgaggagccc tcggaggtcg tcaccgtgcg
    22021 catcgacgac gtcccggaag tcctcccagg cgcgttttcc cggccgcccg ccgaccgacg
    22081 ggtagtgggc ggccagcgcc ggggcccggc cctgaaggac gagccggtgg accgacccca
    22141 tcagccggag ggccacgtag tcctcctcgg gagcgtcctc gcgcccacgc agcgcccgcc
    22201 agaccggccc cccagcctcc gcgtcgtcgg cggcccgtcg caggaggtcc gcgtagagcg
    22261 gagacccaag gcgcccgcac cagcccgcct ggacgcggca gcgctccgcc agcgtccgtc
    22321 ggcctccttc gacgatcacg tcacgaggct cggctctcca ggaacccctt caggttctcc
    22381 aggaccacgc cccacgtcct ctccgaggtc gccttggcct cctcggaggc gaggttggac
    22441 tcctcgaccg tgagcaccgt cccggccccc cgctccgcga gctcccagac gaccacctcg
    22501 tggctctccg ggacgttcgg tcggccggac accgggctcc agtgcgtgtg gacgagacgc
    22561 ctgggcggct cgaactccag gatcgtcccc acgtcctcgt acggcttccc ctggtattcg
    22621 ccgcggtgca cgatcgggct ccccggccgc cagtccgtct ccgtccggac gcccaggaac
    22681 cactgctcga tctggtcggg ggtcgtcagg gccgcccaca cctcgtcgat gggagcgtcg
    22741 atcccgatcg actgctcggt ccgcacgtcc tcggtcacgc ttctacctcc tccgaccatc
    22801 caatcgcatc atccagcgaa gggcaggacg gtgcgggtac ggaagcgatc aacctcgtac
    22861 tcctccagca gcgtcgccgc cctcaccgtg cacgagatgg ggagcagcgg ctccacgcgt
    22921 gaggcgacct cttcgagggt gaccgcgtga tcgccctcca gccgtccgag ggtgcagtgg
    22981 ggaggcgggt cgctcccctc ctctccgtac ggcggatgct gggggtagcg ggcccacaga
    23041 gcccgcatcg tggctcgcag gtcctcgtcc gggtccggca ccgcgtacac gaccagcccg
    23101 gggaaggcag ccacgcgggt gagcgcgaac tcgagcggcg ggcgatccgc gaagaacgac
    23161 cggagacgct cgacgtcgcc gtcggtcacc tcggccgcag ggatccacgg atagaggagc
    23221 gtcagcgaga gggggatctg ctcggggatc cgctccggat agagctcccg atgcgcccga
    23281 gcgagctccg gcgactggtc ctcgaggacg agggtgatgg ccgtctccag cggtcgggac
    23341 ggcggctccg ctacgcggcc cggatcggat gacgtcgcag ccatcccaga acctcctctg
    23401 catgccggtc cggagggaac accgggtacc agacgtgctc gatgcggccg tcgcggacga
    23461 tgagggtcag cctcttgagc aaggtgcggc cggccacctc gaacgtggga agccggagcg
    23521 cccggaccag ctccaatccg gcgtcggaca gcaaggggaa ccggaggcgg aggcggcggg
    23581 cagcctcgcg ctggtcctcg ggagcctgtg tcgacactcc gtagacgccg actcccaacg
    23641 atgcgatctc cgcgtgccgg tcgcggaagg cgcaggactc cgcggtgcat cctcgggcgc
    23701 cggggatgcg gtcccagtcc tccctcaacg gaggctcgcc gggccggccg gtgcgcgggt
    23761 agcagtagac gacggttcgt gagctgtcga ggtcggcgag gcccatcgac cccccctcgg
    23821 tggaggccag cgccacgtcg gggacgcgga cgcccggcag gtgacgggcg ccgccgtcgt
    23881 cctcggggat cggaagagtc ggggggaggg cgacgagctc tcgctcgtcc cgtcgccggc
    23941 gcgctccgcg catgggtcct cccttctgtc cccgaagcat ctcccctggg gcgtcatcgc
    24001 agctgctctt cacactagac agcggaagga gtcacgacca tgccggttcc gtgtcggcga
    24061 ggtcgcggct tcgtccgctc ccctcagaca ccggcgaacg cgcggtcgcg gcacagcctc
    24121 gcgacgcgct tcgccatggc gatgatcgtg aggatagggg gacccccgag cgagcgcggg
    24181 agcagcgacg cgtccgcgac gtacacgttc tcgggcaggc gctcgtcgtg gaagctggtc
    24241 accgtggcct cggacagcgg gagcgccccg cccggatgtc cggcgttgac cgtgccggtg
    24301 aacacgcttt cgggatccgc cccgaagcgc ccgaggatct cggtcgcgat ccccgccgcc
    24361 tcctcgagcc gggatcggtc caccgcggtg aggcgcttgt cgacccgacc gcgcagcgag
    24421 accgagccgg tcccttcgtc ggccagcttg atcatcacgc ccaccatgtc gggcaccggg
    24481 aagcgccaag ccgggttgaa gagcgcgctg atccaatcga agtacggcga caggaggtag
    24541 tgaggccgct gaaccacgaa cggcatctcg atctcgttgc actgccaagc cgcggggatc
    24601 cgcgccgcga tggtgaggac cgggtcgacg aacagaccgg gctcgcaggc gaggttggag
    24661 cgctcgagga tggccggggt cccgaacccg ccggctgcga tcacgatgag gtccgcctcg
    24721 tagagccgac gccccggtcc ccggcccacc aggacgcccc tggcccggcc tccctcgatc
    24781 atgagcctgt cgacccgagc gccagtcacg agctcggcac cgcgggccac cgcctggtcc
    24841 aggaaggccc gggtgtccca cttgatgtcg taggggcagc cgaacacgca ccgcccgcag
    24901 tgccggcact tctcggccag ccccatcttg ggcacgacgg acggctccag gcccatgtcg
//
LOCUS       DASUTW010000289        13466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1131670, whole genome shotgun sequence.
ACCESSION   DASUTW010000289 DASUTW010000000
VERSION     DASUTW010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13466
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131670"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VF058_09920"
     CDS             complement(<1..228)
                     /locus_tag="VF058_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX6208656.1"
                     /translation="MTEIMTATDAEWDKVLSSDKPVLVDFWADWCVPCHMVSPIVEEI
                     GREKSDLMSVAKLNVDDNPQTARRYGVMSIPT"
     gene            complement(287..979)
                     /locus_tag="VF058_09925"
     CDS             complement(287..979)
                     /locus_tag="VF058_09925"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX6208657.1"
                     /translation="MRRAAHLAALGLAVLTVSPSPAPAQPPPLQPPPAYYRPVVTDGM
                     TFPVARSNWHSVITFGRDWHDPRFRMIGGRWRLAGVHEGNDIISEEGTPVYSMTSGIV
                     EAVGWTFYSGTRVGVRGTDGRYYFYAHLSEVAPGIAPGSRVEAGTVLGRVGNTGYGDP
                     GTADEFPPHLHLGIMEGGSWVDPYPTVRRLYRESVRATTRAERRLARLVRNGDTERYE
                     RLLQRLYAEPEE"
     gene            complement(987..1913)
                     /gene="trxB"
                     /locus_tag="VF058_09930"
     CDS             complement(987..1913)
                     /gene="trxB"
                     /locus_tag="VF058_09930"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017620592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX6208658.1"
                     /translation="MTERQVVIIGSGPAGFTAALYAARANLEPLVLKGIEAGGQLMLT
                     TDVENYPGFPEGILGPELMELFEKQAVRFGAEMLQQNATKVDFSGRPLRVWSGDEEHV
                     ARSVIVATGAKAKMLGLPSEQKLLGHGVSTCATCDGFFFRDQELVVVGGGDSAMEEAL
                     FLTRFASKVTVVHRRDELRASKIMQDRAFENPKIGFVWDSTVTDVLGNGKVEGVRLRN
                     VKTGDESELAAEGMFLAIGHTPNTEIFRGQLEMDDVGYLKVEEPTTRTSVEGVFAAGD
                     AVDHIYRQAVTAAGMGCKAAIDAERWLERQGH"
     gene            complement(1967..2806)
                     /locus_tag="VF058_09935"
     CDS             complement(1967..2806)
                     /locus_tag="VF058_09935"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6208659.1"
                     /translation="MSHPHEQLAEYVDGALDASGRAEVEAHLASCGVCRAEVASATSA
                     RQTLRGLPEVEPPPGTTMTVLREARETRTPQRLARPLRIAAAVVLLAGAAAGGIYAYG
                     QLGEGAREPAPAADGGDAAERETEEPAAAPEEGSADQDARSGRVEALFSGPYPQVRRT
                     GTEHTPDSIVSLAEDLRDEAREQLEAGFPQDAFAFYEAFPVASFQPAAREALACAGQG
                     LEPERSVAPFVIEDASWEGRPAWVVSYLQGPEPAAAFDRVQILVVNREECALAHFAQQ
                     RLD"
     gene            complement(2803..3363)
                     /locus_tag="VF058_09940"
     CDS             complement(2803..3363)
                     /locus_tag="VF058_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6208660.1"
                     /translation="MADDRELVRAHLRGDRGAFSTLVERHQRRVYNIAYRMVGPHEAA
                     DATQEVFLTCLRRLSGFRGDSAFTTWLHRVTVNVCLDALRKRSREGPWRDAEGPQPVE
                     RDVADAVVASADVQRALALVPEEFRAPLVLHDLQDLPYEEIAEILGAPLGTVKSRIHR
                     GRVALARALRGEPSPPRDPSNVQETT"
     gene            complement(3356..4843)
                     /locus_tag="VF058_09945"
     CDS             complement(3356..4843)
                     /locus_tag="VF058_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX6208661.1"
                     /translation="MKTEHGRKLAGRYVLDSPLASGGMAEVWSGRDEVLGRPVAVKIL
                     HEHLATDEEFLDRFRREAITAARLSHPCVVRVFDTGVDDGVVFIVMELYEGASLADRL
                     RDGPMSPEEAARIARGILDGLAHAHGQEVVHRDVKPANVLLHGAQVKVSDFGIAKAAH
                     GGDLTTTGQLLGTARYLAPEQVDAASVDHRADLYAVGIVLYEMLTGRAPFEAETAIAE
                     ATLRLTEDPRPPGALRGGIPRDLEDVVMRALARDPDDRFQSAAEMRGALDGSVWTAPP
                     PTPPPSQDEPEPAPSFFRAWMLVPLTAVLVAAAVIVGGLLFGSLELGGPLGVRPRDDP
                     GSPAAPAGGPLEVASVQAHDPEGTGGEHDDEAPFAADGDETTAWTTEGYTTPDLGGIK
                     SGVGLIFDLGSPADVAGLEVATSAPGWTFQVFASGDLAPLEQPASFPQPVADGEGETS
                     FTAEEATTLAFPQARGRYVLLWITELTEVEGRFRALIGEAEFTGG"
     gene            complement(4873..5289)
                     /locus_tag="VF058_09950"
     CDS             complement(4873..5289)
                     /locus_tag="VF058_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208662.1"
                     /translation="MSGRPPSLAERLRDERGILIAALVRLVIFFALLGLVLFEAGSVI
                     WSRLSVQDTADAAATTAVTAYRDSKDVRVAEQEALRTVEDKDETAQMTFFQVHADGSV
                     TVRVRKTAQTLVIQHVGFLDGFTKARGSATIEPPSF"
     gene            complement(5286..6965)
                     /gene="murJ"
                     /locus_tag="VF058_09955"
     CDS             complement(5286..6965)
                     /gene="murJ"
                     /locus_tag="VF058_09955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX6208663.1"
                     /translation="MGKEVLAADDVVTGGPPAEGPPPPGSRPEGLVRSTALMSVGTTL
                     SRVTGFARVAAMTFAIGVAESRLADVYNVANNMPNIIYELVLGGVLSSVFVPVFVEQI
                     ASRTKDEAWHVARAVMTVTMVLLGAVMVIGIVAAPWIVRLYTFRVEAEAQAAAEALGT
                     LFLQLFMPQIVLYGIGAAATGLLNAHRRFVVPMFAPILNNLVVIASFLVFAWMYASAQ
                     PPAPGNITLAQKLVLGVGTTAGVLAMTVALWPSLRRIGFRWRWALDLRHPAIRKLIRL
                     SGWAVGYVIVNQIGLLIVIALAARDQGAYTAYQAAFIFFQLPHAIFSVSIMTALLPSM
                     SERWVGGDRAGFRRILARGVRTSAFIVVPAAAGYMVLARPIVRLLLEHGVTTAESTDL
                     LAGVLTYFALGLFSFSAFMLFLRGFYAMQDTRTPMLVNVFAVALHTGVNFILFPLMGV
                     EGLALGHATAYTFAAIVSALILRRRLGGLEGGYLLAGLGKVVAAAGFTAAAAWGAARL
                     AARAFGVDTLPDQLIQVGVGVLAGLIIFLAAALLFRMEELHLLKRAFLRRR"
     gene            complement(6929..8920)
                     /locus_tag="VF058_09960"
     CDS             complement(6929..8920)
                     /locus_tag="VF058_09960"
                     /inference="COORDINATES: protein motif:HMM:NF039636.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HEX6208664.1"
                     /translation="MTPRILPLLVPLVLLPFPGAIAQEEPSIVELTLVRHSPVGTPDR
                     PLEVSLSAHYPTGGPLRDLTASLWIYHPATSRSAYHQGLADEPPTAPLLIAPYPLRGP
                     LLEGETLEIPIRHDLEELAARGENALYPIKLQLESGGVSVAAVRSAFVFIQEKPLVPL
                     NLSLTVVLDPEIRFSPEVVLLDGSLETAIGEGGSIESLVSALEAEPVRATLALSPLLL
                     EWLDRMSEGYRVRQGDDERSVGADAPEAEAAARMLERIRSLARSAAIEVLALPYASPS
                     VPSLVASGLEEDLRRQISEGRDVVQTLLGVTVSTQTFHPPRSELDRDSVAALAEGGIE
                     SLVLSAERVRPLVPPDSIYSPSATVRLDPTMDAIVPDAQVEAYLTPPPEASTLRAMHL
                     LGEFAAHYFEQPSLLRGASLVLRGDSDGPFLRALLRAIRTTGPRSTWLRPVKASQLAQ
                     AVPPETRGRLRAPAAETFSPGFVEAVGETRDAIDQFESMAETPLDLPERLRTLLLAAE
                     ARRFALNEGEALGFIRMIRATLAEEFDKIDAPSGTSVTLTSQGGVIPVTLRSSAEYPV
                     RLRITLRSSRLEFLQGASREVVLDGEALSLTFPVRAQATGRFPVQILVDTPGGRLISE
                     SEIVVRSTAYNLVALAVTVGAALFLALWWGRRFWRRTTS"
     gene            complement(8948..9379)
                     /locus_tag="VF058_09965"
     CDS             complement(8948..9379)
                     /locus_tag="VF058_09965"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX6208665.1"
                     /translation="MKTRHEVSAGGVVVRGEGREAEVLLASRRVRSGDLVWGLPKGLI
                     EEGESGEDTAIREVQEETGWRASLLRHLRDIEYWFVWEGTRVHKKVRFYLMRAESEDP
                     NGRDHEMEEVRWFPLAEAPSVAGFDSERAVILEAAEAMGAG"
     gene            complement(9376..9828)
                     /locus_tag="VF058_09970"
     CDS             complement(9376..9828)
                     /locus_tag="VF058_09970"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX6208666.1"
                     /translation="MRTAVDVHNFLVEREIPHELVPTQGRLRPPERVAAVLGLEPDQV
                     GRVRLYQGEDLVAALVQADREPSPDLLRAAAGEVSEVDESRATDLTQFLAEATPPVGL
                     PDDTTTILDEGLARQEVLYFPGGEATSLLKIRPDDLVAATGARVAPLT"
     gene            complement(9828..11225)
                     /locus_tag="VF058_09975"
     CDS             complement(9828..11225)
                     /locus_tag="VF058_09975"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019324693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX6208667.1"
                     /translation="MPDPSQEHVADLLSLPPEVDELGTRFQDAGHELYVVGGSVRDAI
                     LRREIEADLDFTTDARPEDVIRVLQPWADARYLQGIRFGTVGAKKGDLRVEITTFREE
                     VYPEPDSRRPAVTFARDVKDDLSRRDFTINAMAVRVPDRLFVDPFRGLYDLGHRRLRT
                     PLDPEISFGDDPLRMLRAARFVSVLGVIPEPEVVDAIRRLRDRLEIVSAERIRDELSK
                     LLLGPEVVAGLRLVLETGLADFFLPELPALRLEQDPVHKHKDVLEHTLAVVERVESRL
                     PVRLAALLHDIGKPATREITPEGVQFHHHDVVGSRMAEERLRALRYPGDVIEKVRRLV
                     EMHLRFHTYGDGWSDGAIRRYVRDAGPLLDDLNMLTRADCTTQNPRKAAMLARLQDEL
                     EARIARLAEEENLARIRPPLDGNQVMERLGVEPGPVVGEALDHLLELRMERGPIGKEE
                     AFRLLDEWWDERRGR"
     gene            11262..11654
                     /locus_tag="VF058_09980"
     CDS             11262..11654
                     /locus_tag="VF058_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208668.1"
                     /translation="MKRLLGALVRVWALPNTLIGLLLGALSFQRPRLDEGVVVFDRAP
                     RGFLWLFGKLSRYSAITFGHVVLSVRAVEGSLRRHERHHVAQYEVLGPLFLPTYLGLF
                     VVKGYRGHPFEVEASEAARRAGPPGAPA"
     gene            complement(11620..12690)
                     /locus_tag="VF058_09985"
     CDS             complement(11620..12690)
                     /locus_tag="VF058_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX6208669.1"
                     /translation="MRVEEYLSFMVEAEASDLHFKVGLPAVIRVNGVLHRTHFPPLTD
                     EDAERLALELLPENRVPQFREGKEIDLGYTSPGGTRFRVNVYRQRGHVSVAIRLVRTR
                     IPTFEELMLPEGAQLLAKEPRGLVLVTGPAGTGKTTTIAAMLGWINQHRRVHIVTIED
                     PIEVMHEDLQAVVEQRELGVDTDSYAAALRAALRQDPDVIFIGEIRDRDTVEAAVQAA
                     ETGHLVIATLHTLDATETVNRVIDLFPAEIQQQARVALAAGLKGVLSQRLLPRADSEG
                     RVPAVEVLINTGRVAERIIDPEQTFTIPDVMAEGEFYAMQTFDQAIVKLVRDSLVTVE
                     DATQAATNPHDLMLALQAAQLV"
     gene            12801..12986
                     /locus_tag="VF058_09990"
     CDS             12801..12986
                     /locus_tag="VF058_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018753158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208670.1"
                     /translation="MAKRMTRAEAGRRGGLSTKRKYGSDFYQEIGRRGGKKGGQTTKK
                     RYGVEFYQKIGRKGGSR"
     gene            complement(13037..13453)
                     /gene="sodN"
                     /locus_tag="VF058_09995"
     CDS             complement(13037..13453)
                     /gene="sodN"
                     /locus_tag="VF058_09995"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HEX6208671.1"
                     /translation="MLSRLLRPRRVVRAHCDLPCGVYDPAQARIEAESVQKIQEKYQD
                     AEKQKKPSETVEDFRARCLIIKEQRADLVKHHLWVLWTDYFKPEHLEKYPDLHDLYWR
                     ATKAAGEAKKSMDPKQGQELLDLIGEIDKIFWETKK"
BASE COUNT         1969 a         4693 c         4859 g         1945 t
ORIGIN      
        1 cgtggggatg ctcatcacgc cgtagcggcg ggcggtctgc gggttgtcgt cgacgttcag
       61 cttggccacc gacatgaggt cggacttctc gcgcccgatc tcctccacga tgggggacac
      121 catgtggcag ggcacgcacc agtccgccca gaagtccaca agcacgggct tgtcggaaga
      181 gagcaccttg tcccactccg cgtccgtggc ggtcatgatc tcggtcatcc gttcgattcc
      241 tcctcggtac tgcgggcgtc tacctgcaac gacgtgcgtg gagcggctat tcctccggct
      301 cagcgtacag cctttggagc agccgctcgt accgctcggt gtccccgttc cgcacgagcc
      361 gcgcgaggcg ccgctccgct cgggtcgtgg cccgcacgct ctcgcggtac aggcgtcgca
      421 ccgtcggata ggggtctacc caggagccgc cctccatgat cccgaggtgg aggtggggcg
      481 ggaactcgtc cgccgtcccg gggtccccgt agcccgtgtt gcccacccgg cccaggaccg
      541 tgccggcctc gacccgtgac cccggcgcga tgccgggggc gacctcggag aggtgcgcgt
      601 agaagtagta gcggccgtcc gtccctcgga cgcccacccg cgtccccgag tagaacgtcc
      661 agcccaccgc ctccacgatc ccggaagtca tcgagtagac cggcgttccc tcctcggaga
      721 tgatgtcgtt cccctcgtgc acgccggcca gccgccaccg ccccccgatc atgcggaacc
      781 gaggatcgtg ccagtcgcgg ccgaaggtga tgaccgagtg ccagttcgaa cgagccaccg
      841 ggaaggtcat cccgtccgtg acgacgggcc ggtagtaggc ggggggtggt tggaggggag
      901 ggggctgggc cggggccggg gatggggaga cggtcaggac agcgagcccg agggccgcga
      961 ggtgggcggc gcggcgcatc ccggggtcag tggccctggc gctcgagcca ccgctcggcg
     1021 tcgatcgcgg ccttgcaccc catcccggcc gcggtcacgg cctgccggta gatgtggtcc
     1081 actgcgtcgc cggcggcgaa gacgccctcc acgctggtcc gcgtcgtcgg ctcctcgacc
     1141 ttgaggtagc ccacgtcgtc catctccagc tgtccccgga agatctcggt gttgggcgtg
     1201 tgtccgatcg ccaggaacat gccctccgcg gcgagctcgg actcgtctcc ggtcttgacg
     1261 ttccgaagcc ggacgccctc gaccttcccg ttccccagga catccgtcac ggtcgagtcc
     1321 cagacgaacc cgatcttggg gttctcgaag gctcggtcct gcatgatctt cgaggccctg
     1381 agctcgtcac gccggtgcac gaccgtgacc ttcgacgcga agcgggtgag gaagagcgcc
     1441 tcctccatcg ctgagtcccc gcccccgacg accaccagct cctggtcgcg gaagaagaag
     1501 ccgtcgcacg tggcgcaggt cgagacgccg tgtcccagca gcttctgctc cgaggggagg
     1561 cccagcatct tggccttggc cccggtggcc acgatcaccg agcgggcgac gtgctcctcg
     1621 tcccccgacc agacccgcaa cgggcgcccg gagaaatcca ccttggtggc gttctgctgc
     1681 agcatctccg ccccgaagcg gaccgcctgc ttctcgaaga gctccatgag ctcggggccg
     1741 aggatcccct cggggaaccc cgggtagttc tcgacgtcgg tggtgagcat cagctggccg
     1801 ccggcctcga tgcccttgag gacgagcggc tcgagattgg cccgagccgc gtagagggcg
     1861 gcggtgaagc ccgccggccc ggacccgatg atgacgacct gtcgctcggt cacttgccca
     1921 ttctcccagg agacgcccga tcatccaacg ccgggagggc gttcgtttag tccaggcgct
     1981 gctgggcgaa gtgggccagc gcgcactcct cccgattcac cacgaggatc tggacccgat
     2041 cgaaggcggc ggcgggctcg ggcccctgga ggtacgagac gacccaggcc ggccggccct
     2101 cccacgaggc gtcctcgatc acgaacgggg cgacgctgcg ctcgggctcc aggccctggc
     2161 cggcgcaggc cagggcttcc cgggccgccg gctggaacga ggcgaccggg aaggcctcgt
     2221 agaaggcgaa ggcgtcctgt gggaagccgg cctcgagctg ctcccgcgcc tcgtcccgga
     2281 ggtcctcggc cagcgacacg atcgagtccg gggtgtgctc ggtcccggtg cggcggacct
     2341 gggggtacgg tccgctgaag agcgcctcca cccgaccgct ccgggcgtcc tggtccgcgc
     2401 ttccctcctc gggggccgcg gccggctcct ccgtctcccg ctccgccgca tcgcctccgt
     2461 ccgcggccgg ggcgggctcc ctggcgccct ccccgagctg accgtacgcg tagatgcctc
     2521 cggcggcggc cccggccaag aggaccacgg cggctgcgat gcgcaggggc cgagccaagc
     2581 gctggggcgt ccgggtctcg cgggcctccc ggaggacggt catcgtcgtt ccggggggtg
     2641 gctcgacctc ggggaggcct cggagggtct gccgggccga ggtggccgag gccacctcgg
     2701 cccggcagac cccacacgaa gccaggtgcg cctcgacctc ggctcgcccc gaggcgtcga
     2761 gggccccgtc cacgtactcg gccagctgct cgtggggatg gctcacgtcg tctcctgcac
     2821 gttcgacgga tcccgagggg gtgagggttc ccctcggagg gcccgggcca gggccacccg
     2881 gccccggtgg atcctggact tcacggtgcc gagcggcgcc ccgaggatct cggcgatctc
     2941 ctcatagggg aggtcctgga ggtcgtggag gacgagcggc gcgcggaact cctcgggaac
     3001 gagggcgagg gctcgctgga cgtccgcgga ggccacgacc gcgtccgcaa cgtcccgctc
     3061 cacgggctgg ggcccctcgg cgtcacgcca cggtccctcc cgcgaccgct tccggagggc
     3121 gtcgaggcag acgttcaccg tcacccggtg cagccaggtg gtgaaggccg agtcccctcg
     3181 gaagccggag agcctccgca ggcacgtcag gaacacctcc tgggtggcgt cggccgcctc
     3241 gtgcggcccg accatccggt acgcgatgtt gtagacgcgg cgctgatgtc gctccacgag
     3301 cgtggagaag gctccccggt cgccccgaag gtgggcccgg acgagctccc ggtcgtcagc
     3361 cacccgtgaa ctcggcctcg ccgatcaggg cccggaaccg gccctcgacc tcggtcagct
     3421 ccgtgatcca gagcaggacg tagcgtccac gggcctgggg gaaggcgagc gtcgtggcct
     3481 cctcggcggt gaagctggtc tccccctccc cgtccgcgac gggctgaggg aaggatgcgg
     3541 gctgctccag cggggcgagg tcgccggagg cgaagacctg gaacgtccat cccggcgcgc
     3601 tcgtggccac ctcgaggccc gccacgtccg cgggcgatcc gagatcgaag atgaggccga
     3661 cgcccgactt gatccctccc aggtccggcg tcgtgtagcc ctcggtcgtc caggcggtcg
     3721 tctcgtcgcc gtcggcggca aagggcgcct cgtcgtcgtg ctcgcccccc gtcccctcgg
     3781 gatcgtgggc ctggaccgac gccacctcga gcgggccgcc ggccggcgcg gccggggagc
     3841 ccgggtcgtc gcgcgggcgg accccgaggg gaccgccgag ctccaggctc ccgaacagca
     3901 gaccgccgac gatcaccgcc gccgccacca ggacggccgt cagcggcacg agcatccagg
     3961 cccggaagaa cgagggggcc ggctcgggct cgtcctggga cgggggcggg gtgggtgggg
     4021 gagcggtcca gacggacccg tcgagcgcgc cgcgcatctc cgccgccgac tggaaccggt
     4081 cgtccgggtc ccgggccagg gcccgcatga cgacatcctc caggtcccgg gggatgcccc
     4141 ctcggagagc accggggggg cggggatcct cggtgaggcg cagagtggcc tccgcgatgg
     4201 cggtctcggc ctcgaagggc gcgcggccgg tcagcatctc gtagaggacg atgcccacgg
     4261 cgtagagatc cgcgcgatgg tcgaccgagg cggcgtccac ctgctcggga gcgaggtagc
     4321 gggcggtgcc gagcagctgc cccgtggtgg tcaggtcgcc gccgtgggcc gccttcgcga
     4381 tcccgaaatc gctcaccttc acctgggccc cgtggagcag gacgttggcg ggcttgacgt
     4441 cgcggtggac gacctcctgg ccgtgggcgt gggcgaggcc gtcgaggatg ccgcgggcga
     4501 tgcgcgccgc ctcctccggc gacatggggc cgtcgcgcag cctgtcggcc agcgaggccc
     4561 cctcgtacag ctccatcacg atgaagacca ccccgtcgtc cacgccggtg tcgaacactc
     4621 gcacgacgca ggggtgggag aggcgggccg ccgtgatcgc ctcgcgccgg aaccggtcga
     4681 ggaactcctc gtcggtggcg aggtgctcgt gcaggatctt gacggcgacc ggacggccca
     4741 ggacctcgtc gcgcccgctc cagacctccg ccatgcctcc cgaggcgagg ggcgagtcga
     4801 ggacgtagcg gccggcgagc ttccgcccgt gctccgtctt catcgagttc agtgtagttg
     4861 gtgccctcgg ggtcagaagg agggcggctc gatcgtggcg gacccccggg ctttggtgaa
     4921 cccgtccaga aagccgacgt gctggatcac cagcgtctgg gccgtcttcc ggacgcggac
     4981 cgtgacgctg ccgtcggcgt ggacctggaa gaaggtcatc tgggccgtct cgtccttgtc
     5041 ctcgaccgtc cgaagcgcct cctgctcggc cacccggacg tccttgctgt cccggtacgc
     5101 ggtgaccgcg gtggttgcgg cggcgtcggc agtgtcctgg acggagagcc tcgaccagat
     5161 cacggacccc gcctcgaaca ggaccagccc gagcagggcg aagaagatca cgagccgcac
     5221 gagggctgcg atcaggatgc cgcgctcgtc gcgcagtctc tcggcgaggg agggggggcg
     5281 gccgctcatc gccggcgcag gaatgctctc ttcaacaggt gcaactcctc catccggaag
     5341 agcagggccg cagcgaggaa gattataagc cccgccagga cgcccacccc cacttggatc
     5401 agctgatccg gcagcgtgtc gacgccgaag gcgcgggcgg cgaggcgggc cgctccccac
     5461 gcggcggccg cggtgaaccc cgcggcggcg accaccttcc ccagccccgc caggaggtag
     5521 ccgccctcca gaccgcccag ccgcctccgc aggatgagcg cggagacgat cgccgcgaac
     5581 gtgtaggccg tggcatgccc cagggccagc ccctccacgc ccatgagcgg gaagaggatg
     5641 aagttgaccc ccgtgtgcag cgcgaccgcg aagacgttga cgagcatcgg ggtccgggtg
     5701 tcctgcatcg cgtagaagcc cctcaggaac agcatgaacg ccgagaacga gaacaggccg
     5761 agggcgaagt acgtgagcac cccggccagc agatcggtcg actccgcggt cgtgacgccg
     5821 tgctcgagca gcaggcggac gatcggacgc gccagcacca tgtacccggc cgcggcgggc
     5881 acgacgatga acgccgaggt ccggacgccg cgggctagga tgcggcggaa gccggcccgg
     5941 tcgccaccca cccagcgctc ggacatggac ggcaggaggg ccgtcatgat cgagaccgag
     6001 aagatcgcgt gcggcagctg gaagaagatg aaggcagcct ggtaggcggt gtaggcgccc
     6061 tgatcccggg cggcgagggc gatcacgatc agcaggccga tctggttcac gatcacgtag
     6121 cccaccgccc agcccgagag ccggatcagc ttccggatcg cggggtggcg gaggtcgagc
     6181 gcccaccgcc accggaagcc gatccgccgc agcgagggcc agagggccac ggtcatggcc
     6241 agcacaccgg ccgtggtccc cacgccgagg accagcttct gggccagggt gatgttgccc
     6301 ggggctgggg gctgggcgct cgcgtacatc caggcgaaca cgaggaacga tgcgatgacc
     6361 acgaggttgt tgaggatggg agcgaacatc gggaccacga accgccggtg ggcgttgagc
     6421 aggccggtgg ccgccgcccc gatcccgtac aggacgatct ggggcatgaa cagctggagg
     6481 aacagggttc cgagcgcctc cgcggccgcc tgggcctcgg cctccacccg gaaggtgtag
     6541 aggcgaacga tccagggggc ggccacgatc ccgatcacca tgacggcccc gaggaggacc
     6601 atcgtgacgg tcatcacggc cctcgccacg tgccacgcct cgtccttcgt ccgggaggcg
     6661 atctgctcca cgaagaccgg tacgaacacc gaagagagga ccccgcccag caccagctcg
     6721 tagatgatgt tgggcatgtt gttggcgacg ttgtagacgt cggccaggcg cgactcggcc
     6781 acgccgatgg cgaaggtcat cgcggcgacc cgagcgaagc cggtgacccg cgagagggtg
     6841 gttccgacgg acatgagggc ggtgctccgc acgagcccct cgggcctcga gcccggcgga
     6901 ggtggcccct ccgcgggcgg gcccccggtc acgacgtcgt ccgccgccag aacctccttc
     6961 cccaccacag cgcgaggaac agcgccgcgc ccaccgtcac ggccagggcc acgaggttgt
     7021 aggccgtgga ccgcaccacg atctccgact cgctgataag cctgccgccc ggcgtgtcca
     7081 cgaggatctg gacggggaag cgccccgtgg cctgggcccg gacggggaag gtgagggaca
     7141 gggcctcgcc gtccaggacg acctcgcggg aggccccctg caggaactcg agccgagacg
     7201 accgcaaggt gatgcggagg cgaaccgggt actcggccga gctccggagg gtgacgggga
     7261 tcaccccgcc ctgggaggtc agcgtcaccg aggttcccga gggagcgtcg atcttgtcga
     7321 actcctcggc cagcgtggcg cggatcatcc ggatgaagcc cagcgcctcc ccctcgttga
     7381 gcgcgaagcg cctggcctcg gcggccagga gcagcgtcct gagccgctcg ggcaggtcga
     7441 ggggggtctc cgccatcgac tcgaactggt cgatggcgtc ccgggtctcg cccacggcct
     7501 ccacgaaccc cggggagaag gtctcggccg ccggggcgcg gagccgaccc cgagtctccg
     7561 ggggcaccgc ctgggccagc tgggaggcct tcacggggcg gagccaggtg gagcgggggc
     7621 ccgtggtgcg gatcgctcgg aggagcgcgc ggaggaaggg cccgtcgctg tcccctcgca
     7681 ggaccaggga tgctccccgc agcaggctcg gctgctcgaa gtagtgagcg gcgaactccc
     7741 ccaacaggtg catcgcgcgc agcgtggacg cctcgggcgg gggggtgagg taggcctcga
     7801 cctgcgcgtc ggggacgatc gcgtccatcg tgggatcgag gcgcacggtg gcggaggggc
     7861 tgtagatcga gtccggcggg acgagggggc ggacgcgctc agcgctcagc acgagggact
     7921 cgatgccgcc ctccgcgagc gccgcgacgc tgtccctgtc gagctcggac cggggcgggt
     7981 ggaaggtctg ggtcgagacg gtgacgccca gcagggtctg gaccacgtct ctcccctcgc
     8041 tgatctggcg gcggaggtcc tcctcgagcc cggatgccac gagcgaaggg accgaggggc
     8101 tggcgtaggg gagggcgagg acctcgatgg cggcgctcct ggccagcgat cggatccgct
     8161 ccagcatgcg ggcggccgcc tccgcctccg gggcgtcggc tccgaccgat cgctcgtcat
     8221 ctccctgtcg cacgcggtac ccctcgctca ttcggtcgag ccactcgagc aggagggggg
     8281 agagggcgag cgtcgcccgc acgggctcgg cctccagcgc ggagacgagc gattcgatcg
     8341 agcctccctc gccgatcgcc gtctccagcg agccgtccag gagcacgacc tccggcgaga
     8401 agcggatctc gggatcgagc acgacggtca gggagaggtt gagggggacc agcggcttct
     8461 cctggatgaa gacgaacgct gagcgaacgg ccgccacgga gacgccgccg gactcgagct
     8521 ggagcttgat cgggtacagc gcgttctcgc cccgggctgc gagctcctcg aggtcgtggc
     8581 ggatggggat ctccagcgtc tcgccctcca ggaggggccc tcgcagcggg tacggggcga
     8641 tcagcaaggg ggcggtgggc ggctcgtcgg ccaggccctg atggtaggcc gaccgtgagg
     8701 tggcgggatg gtagatccac aggctggccg tcaaatcacg cagtggtccc ccagtggggt
     8761 agtgggcgct cagcgagacc tcgagcgggc gatcgggcgt gccgaccggg ctgtggcgga
     8821 cgagggtcag ctcgacgatc gaaggctcct cctgcgcgat cgcgcccggg aaggggagaa
     8881 gcacgagcgg caccagcagg ggcaggatgc gaggcgtcac ggcgcggtca ttgtatgccc
     8941 ggctcggtca gccggccccc atggcctcgg ccgcctccag gatcaccgca cgctccgaat
     9001 cgaacccggc cacggagggg gcctcggcca gggggaacca ccggacctcc tccatctcgt
     9061 ggtcgcgccc gttcgggtcc tcgctctctg ccctcatgag gtagaagcgc accttcttgt
     9121 gcaccctggt cccttcccag acgaaccagt actcgatgtc gcggaggtgt cgcaggagcg
     9181 aagcccgcca acccgtctcc tcctgtacct cccgaatcgc ggtgtcctcg cccgactcgc
     9241 cctcctcgat caggcccttg gggaggcccc acacgaggtc gccggagcgg acccggcggg
     9301 aggcgaggag gacctcggcc tcccggccct cgccgcggac gaccacgccc ccggccgata
     9361 cctcgtgcct cgtcttcacg tgagaggcgc cacgcgggcc ccggtcgcgg cgacgaggtc
     9421 gtccggtcgg atcttcagga gcgaggtcgc ctccccgccg gggaagtaga ggacctcctg
     9481 cctggcgaga ccctcgtcga ggatcgtcgt ggtgtcgtcg gggaggccca ccgggggtgt
     9541 ggcctcggcc aggaactgcg tgaggtcggt ggcgcgggac tcgtcgacct cggacacctc
     9601 tcctgcggcg gctctgagaa ggtccggcga cggctcgcgg tctgcctgga ccagcgcggc
     9661 cacgaggtct tcaccctggt agaggcggac gcgtcccacc tggtcgggct ccagccccag
     9721 gaccgcggcg acgcgctcgg gggggcgcag gcggccttgc gtggggacga gttcgtgggg
     9781 gatctcccgc tccacgagga agttgtggac gtcgacggcc gttcgcatca gcgtcccctc
     9841 cgttcgtccc accactcgtc gaggagccgg aacgcctcct ccttcccgat cgggccccgc
     9901 tccatccgga gctcgaggag gtggtccagc gcctcgccga cgacgggccc gggctcgacg
     9961 cccagacgct ccatcacctg gttcccgtcg agcgggggac ggatccgagc caggttctcc
    10021 tcctccgcca ggcgggcgat ccgggcctcg agctcgtcct ggagcctggc cagcatcgct
    10081 gccttgcgtg ggttctgcgt cgtgcagtcg gccctcgtca gcatgttgag gtcgtcgagg
    10141 aggggcccgg cgtcccggac gtatcggcgg atggccccgt cgctccatcc gtcgccgtac
    10201 gtgtggaagc gcaggtgcat ctcgaccagc cgccgaacct tctcgatcac gtcgcccggg
    10261 tagcggagcg cgcgcagacg ctcctccgcc atccgggacc ccaccacgtc gtgatggtgg
    10321 aactgcacgc cctcgggagt gatctcgcgg gtggcgggct tcccgatgtc gtggaggagc
    10381 gcggccagcc ggaccggcag cctcgactcc acccgctcca ccaccgccag cgtgtgctcg
    10441 agcacgtcct tgtgcttgtg gacggggtcc tgctccaggc ggagcgcggg gagctcgggg
    10501 aggaagaagt cggcgagccc cgtctccagg acgagccgga gaccggccac gacctcgggc
    10561 ccgagcagca gcttcgagag ctcgtcccgg atccgctcgg ccgagacgat ctcgaggcga
    10621 tcgcggagcc gccgtatcgc gtccacgacc tcgggctccg ggatcacgcc gaggaccgag
    10681 acgaacctcg cggcgcgcag catccgcaac gggtcgtcgc cgaacgagat ctcgggatcg
    10741 agcggagtcc gtaggcgccg gtgtccgaga tcgtagaggc cgcggaacgg atccacgaag
    10801 agccggtcgg ggacgcgcac cgccatggcg ttgatcgtga agtcccggcg agagagatcg
    10861 tccttgacgt ctcgggcgaa cgtgacggcg ggccggcggg agtcgggctc cggatacacc
    10921 tcctctcgga aggtcgtgat ctcgacgcgg aggtccccct tcttcgcccc cacggtgccg
    10981 aagcggatcc cctgcaggta ccgcgcgtcc gcccacggct ggaggacccg gatgacgtcc
    11041 tcggggcggg cgtcggttgt gaagtcgaga tcggcctcga tctcccgccg gaggatcgcg
    11101 tcccgcacgg acccgcccac gacgtacagc tcgtggccgg catcctggaa gcgggtcccc
    11161 agctcgtcca cctcgggggg caggctcagc aggtcggcga cgtgctcctg ggaggggtcc
    11221 ggcacaacga ggaggatacc ctagggcgag gaggccacca cgtgaagaga ctgctcggcg
    11281 ccctggtgcg cgtctgggcg ctccccaaca ccctgatcgg cctgctgctg ggggccctct
    11341 cgttccaacg tccccggctc gacgagggcg tggtggtctt cgaccgggcc ccccgggggt
    11401 tcctctggct cttcgggaag ctctcccgct actcggcgat cacgttcggg cacgtggtcc
    11461 tctcggtgcg agcggtcgag gggagcctcc gccgccacga gcgccaccat gtcgcgcagt
    11521 acgaggtgct gggccccctc ttcctcccga cgtacctggg gctgttcgtc gtcaagggct
    11581 accgagggca cccgttcgag gtcgaggcgt cggaggccgc tagacgagct gggccgcctg
    11641 gagcgccagc atgagatcgt gcgggttcgt ggccgcctgc gtcgcgtcct ccacggtgac
    11701 gaggctgtcc cggacgagct tcacgatggc ctggtcgaag gtctgcatcg cgtagaactc
    11761 gccctcggcc atgacgtcgg ggatcgtgaa ggtctgctcg gggtcgatga tccgctcggc
    11821 gacccgccca gtgttgatca gcacctcgac cgccggcacc cggccctcgc tgtccgctcg
    11881 cgggagcagg cgctgggaga ggacgccctt cagccccgcg gccagggcca cacgggcctg
    11941 ctgctggatc tcggcgggga agaggtcgat cacccggttg acggtctcgg tggcgtcgag
    12001 ggtgtggagg gtcgcgatca cgaggtggcc ggtctcggcc gcctgcaccg ccgcttcgac
    12061 cgtgtcgcgg tcgcggatct cgccgatgaa gatcacgtcg gggtcctgcc ggagggccgc
    12121 gcggagggcc gcggcgtagg agtcggtgtc cacccccagt tcgcgctgct cgaccaccgc
    12181 ctggaggtcc tcgtgcatga cctcgatcgg gtcctcgatc gtgacgatgt ggacccggcg
    12241 gtgctggttg atccagccga gcatcgcggc gatcgtcgtt gtcttcccgg tccccgcagg
    12301 gcccgtcacc aacacgagcc ctctcggctc cttggccagg agctgcgcgc cctcggggag
    12361 catgagctcc tcgaacgtgg ggatcctggt ccggaccagg cggatcgcga cgctcacgtg
    12421 cccccgctgc cggtagacgt tcacccggaa cctggtcccg ccgggggatg tgtacccgag
    12481 gtcgatctcc ttcccctcgc ggaactgcgg tacgcggttc tccgggagga gctcgagggc
    12541 caggcgctcg gcgtcctcgt cggtcagcgg ggggaagtgg gtgcggtgca gcacaccgtt
    12601 cacgcggatg accgccggca gcccgacctt gaagtgcaga tcggaggcct cggcctccac
    12661 catgaacgag aggtactcct cgaccctcat atccgcttcc atcatcggcc ggatgcccgc
    12721 ctcccttaac ggacgggttt gccgaggcaa gccgggggta cctctaggcg tgcacctgcg
    12781 agcagaagga ggggacgaag ttggccaagc gcatgacgcg agcggaggca ggccgccgtg
    12841 gcggcctctc caccaagagg aagtacggga gcgacttcta tcaggagatc ggtcggcgcg
    12901 gcgggaagaa gggcggccag accacgaaga agcgctacgg cgttgagttc taccagaaga
    12961 tcggcaggaa gggcggctcc cggtaggggt cacccgcggg gccggggggc ggcccccggc
    13021 ccgcgggcac ccaccttcac ttcttggtct cccagaagat tttgtcgatc tccccgatga
    13081 ggtccagaag ctcctggccc tgcttcggat ccatggactt cttggcctcg ccggccgctt
    13141 tcgtggccct ccagtacagg tcgtggaggt ccgggtactt ctcgagatgc tccggcttga
    13201 agtagtccgt ccacagcacc cacaggtggt gcttgacgag gtccgcgcgc tgctccttga
    13261 tgatgaggca gcgcgccctg aagtcctcca ccgtctcgga cggcttcttc tgcttctccg
    13321 cgtcttggta cttctcctgg atcttctgga ccgattccgc ctcgatcctg gcctgggccg
    13381 ggtcgtagac gccgcagggg aggtcgcagt gcgcgcggac gacccgcctg ggcctcagca
    13441 gtcggctgag catgcctcct ccttcc
//
LOCUS       DASUTW010000290         2921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1002558, whole genome shotgun sequence.
ACCESSION   DASUTW010000290 DASUTW010000000
VERSION     DASUTW010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002558"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..1860
                     /locus_tag="VF058_10000"
     CDS             241..1860
                     /locus_tag="VF058_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX6208672.1"
                     /translation="MEREVDRRREALIRAGLTLASELSLPVVLQKLTELACLVADARY
                     GALGVLDRSGRRIERFVTHGVTSEERRAIGDLPKGRGILGLLIREARPLRLDRIQDDP
                     RSVGFPPHHPVMTSFLGVPIMVRGNVFGNLYLTEKRGGDRFTEEDEEAVMTLATQAAV
                     AVENARLYEEAQTARERLEAVGSVTAAILEGRETDDVLRLIAGHARDLAGGDLSSVTV
                     PVGGGRLGLRVVVGELADQLEGEEYESEGSIAEEVMRTGRPLMIPDLSRDPRKGQPVV
                     RIGRIGPGIYLPLTVGDRRFGTLLVANLLGGRTFDQEDAEVVRMFAAQAAVALEHARF
                     REELQRLAVLEDRERIAQELHDGVIQSLFAVGMSLQATAGMAKDPETSERVNHAVDAV
                     DGAIRDLRNYIFGLRPGALADRQLALSLEELAKSFDESTGLTVIASIDRDVAARLAGR
                     EAEVLQAAREAISNAVRHSGGETVRVSLRQKGKDAVLEVADDGSGFDPAHVEGRGQGL
                     GNLLHRAESLEGRLQIISDEEGTTVRITLPL"
     gene            1875..2540
                     /locus_tag="VF058_10005"
     CDS             1875..2540
                     /locus_tag="VF058_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010985800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6208673.1"
                     /translation="MADQPIRVMIVDDHEVVRQGVRSLLEAADDVVVAGEASGVSESV
                     ELAENTRPDVVIMDVRLIDGSGIEACREIRARRPETKVVMLTSFADDEALFASIMAGA
                     SGYVLKQVRGGDLLSSIRQVAQGHSLLDPAVTGPVLERLRKGKHLMKDERLARLSPQE
                     ERILTQVADGKTNREIGEDLGLAEKTVKNYVSSILSKLEVARRAEAAAYLARHTGTPG
                     DQP"
     gene            complement(2542..>2921)
                     /locus_tag="VF058_10010"
     CDS             complement(2542..>2921)
                     /locus_tag="VF058_10010"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HEX6208674.1"
                     /translation="AAAAADLDEEGRTAAVSAVLTTLMERLPRAHREHLMSHLPEDVR
                     SLGHHPRAPGEAATSIRTQREFAEAVEELARLDGAEGITGPVRALVVSGAVLAALRRL
                     IPEEDHDVAAVLPGELRDLWKAA"
BASE COUNT          428 a         1016 c         1072 g          405 t
ORIGIN      
        1 cccgtgctcg agcacggcca gggacccgat ctcctggtac tgcatgcggc ccgcggcgtc
       61 gagcagcgac tcgtcccggc tccccgccac gacctccggg cggtacaacg ctcgaacctt
      121 catcacacac ctcctccatc gctcgcatgg ccaccgtagg caggcccggt cccggccgac
      181 aggtccgaag ggcccctccg cgtcgggtcc tccgccccgt cctgctaccc tcgaaccggc
      241 atggagcggg aggtcgatcg tcgtcgcgag gcgctgatca gagccgggct caccctcgca
      301 tccgagctct cgctccccgt tgttctccag aagctgaccg agctcgcctg tctcgtggcc
      361 gacgcccggt acggcgcgct cggggttctc gatcgttccg gccgccggat cgagcgcttc
      421 gtcacccacg gggtcacgag cgaggagcga cgtgcgatcg gggacctgcc gaagggccgg
      481 ggcatcctcg ggctcctcat cagggaggct cgacctctcc ggctggatcg gatccaggac
      541 gacccgcggt cggtgggctt ccctccccac caccccgtga tgacgagctt cctcggcgtc
      601 cccatcatgg tccgggggaa cgtcttcggg aacctctacc tcaccgagaa gcggggcggg
      661 gaccgcttca ccgaggagga cgaggaagcc gtgatgaccc tggccaccca ggccgcggtc
      721 gcggtggaga acgcccggct ctacgaggaa gcgcagacgg cccgggagcg cctcgaggcc
      781 gtcggaagcg tcaccgccgc gatcctcgag ggccgggaga ccgacgacgt gctccggctg
      841 atcgcgggcc acgctcgcga cctggcgggg ggggacctgt cgtcggtcac cgtccccgtt
      901 ggcggggggc ggctgggcct ccgcgtggtg gtgggcgagc tcgccgacca gctcgagggc
      961 gaggagtacg agtccgaggg ctccatcgcc gaggaggtga tgcgcacggg ccggcccctc
     1021 atgatccccg acctctcccg ggatcccagg aagggccagc cggtggtgcg gatcggccgg
     1081 atcggacccg ggatctacct tcccctcacg gtcggggatc gtcgcttcgg gacgctgctg
     1141 gtggcgaacc tcctgggcgg acggacgttc gaccaggagg acgcggaggt cgtgcggatg
     1201 ttcgcggccc aggccgcggt cgccctcgag cacgcgcggt tccgggagga gctccagcgg
     1261 ctggccgtcc tggaggatcg ggagcggatc gcccaggagc tccacgacgg ggtgatccag
     1321 tcgctgttcg cggtggggat gtcgctccag gccacggccg ggatggccaa ggacccggag
     1381 acgagcgagc gcgtgaacca cgcggtggac gcggtggatg gcgcgatccg ggatctccgg
     1441 aactacatct tcgggctccg tcccggagcg ctcgcggatc gccagctggc gctctcgctg
     1501 gaggagctgg ccaagagctt cgacgagagc accgggctca cggtgatcgc atcgatcgat
     1561 cgcgacgtcg cggcgcgcct cgcgggccgg gaggcggagg tcctccaggc cgcgcgggag
     1621 gcgatctcga acgccgtccg gcactcggga ggagagaccg tgcgggtcag cctccggcag
     1681 aaggggaagg acgcggtcct ggaggtggcg gacgacggga gcgggttcga cccggcccac
     1741 gtcgaagggc gcggccaggg cctggggaac ctgctgcacc gcgcggagtc tctcgaaggc
     1801 cgccttcaga tcatctccga cgaggagggc accaccgtac gcatcaccct ccccctgtga
     1861 gaacattggc gacgatggcc gaccagccga tccgcgtgat gatcgtcgac gaccacgagg
     1921 tcgttcgcca gggcgtgcgg tcgctgctgg aggcggcgga cgacgtggtc gtcgcgggcg
     1981 aggcatccgg ggtgtccgag tcggtggagc tcgccgagaa cacccgcccc gacgtcgtga
     2041 tcatggacgt ccgcctgata gacgggagcg gcatcgaggc gtgccgcgag atccgtgctc
     2101 ggcgtcccga gacgaaggtc gtgatgctga cgtcgttcgc cgacgacgag gcgctcttcg
     2161 cctcgatcat ggccggggcg tcgggctacg tcctgaagca ggtgcggggg ggcgatctcc
     2221 tcagctcgat ccgtcaggtc gcccagggcc attcgctgct cgacccggcc gtgaccggcc
     2281 ctgtgctgga gcggctgcgc aagggcaagc acctgatgaa ggatgagagg ctggcccggc
     2341 tctcccctca ggaggagagg atcctcaccc aggtggccga cggcaagacg aaccgcgaga
     2401 tcggcgagga cctcgggctg gcggagaaga ccgtgaagaa ctacgtctcg agcatcctgt
     2461 ccaagctcga ggtggcgcgg cgcgccgagg ccgccgcgta cctggcccgc cacaccggga
     2521 cgccgggcga ccagccctga cctaggcggc cttccacaga tcgcgaagct ctcccgggag
     2581 caccgccgcc acgtcgtggt cctcctcggg gatcagccga cgcagcgccg ccagtaccgc
     2641 tccggagacg acgagggccc ggaccgggcc cgtgatgccc tcggctccgt cgagccgtgc
     2701 cagctcctcc accgcctccg cgaactcccg ctgcgtgcgg atcgaggtcg ccgcctcccc
     2761 gggagcccga gggtgatgcc cgagcgagcg cacgtcctcg gggaggtggc tcatgaggtg
     2821 ctcgcggtga gccctgggga ggcgctccat cagggtcgtg aggacggccg agaccgccgc
     2881 cgtccgcccc tcctcgtcga ggtcggcggc ggcggccgcg g
//
LOCUS       DASUTW010000291         3188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_355209, whole genome shotgun sequence.
ACCESSION   DASUTW010000291 DASUTW010000000
VERSION     DASUTW010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355209"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VF058_10015"
     CDS             complement(<1..675)
                     /locus_tag="VF058_10015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6208675.1"
                     /translation="MTTSVDAGTDPIDSLYSARLVARRRLLSRLVKARDLERQRIAAE
                     IHDEPLQVLSTISTRLQRLRQAQEDPALRSEIGGIQETLTAAIEHLRGLTFELARGPR
                     EEFGLLAELRAMLERAAEDADLEARLSERVIEEPPFETASAAYRILQEALANIQKHAR
                     ATRVDVAVESDDGLFVRIEDDGVGFEPEGVVPSPSHMGIAMMRQRAMAAGGWLRVESE
                     AGKGTAI"
     gene            837..2495
                     /locus_tag="VF058_10020"
     CDS             837..2495
                     /locus_tag="VF058_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208676.1"
                     /translation="MGTRSRARLATTAILASFGAVAVLASRPNAPFTPFLHPGDRSPL
                     PLRELSRLLGLDALDRDVAALVGAALLVASAIAFVYAARVAWRGDLSVSRVIWVGVAL
                     HVLAFVLPLFLSRDVYSYAYYGRMVSRYGVNPYLFTPGQFLQDPFYLYVSHFWIDSPS
                     VYGPAFSALAGWATGIASSVPGTVDAFKLVAAVAGVGTMLAVVAAARQVRPRRAAFAG
                     LLIGWNPVLIFHNVAGGHNDALVGLAVALAALLVLSRRDVLATGVLVLGCLVKATAVI
                     PLAMLVVGSVAARPKGRRLRSLAAHAAMGLVVGLPFVIRFFSLEDPSMGQLELATRQG
                     WLAPSRFLVVTLREAVNAVAGETAGDLTSALIRIVFPVVLALVLVSIGRHVARRPDRI
                     RPELIVAAMGWATLFGLMMAPILLPWYAAWMIPLAWLLPKPARGGAIFISVALVITEL
                     MSEPTRSPRVWEVMVIWLHWVATPIVLAVLVRLLLELRARLRMGPAEGASDPLLTERP
                     ILRSRVLRILDRAVSGREDVADEGERPGEGGAASSRGEREPVGG"
BASE COUNT          367 a         1167 c         1159 g          495 t
ORIGIN      
        1 gatcgccgtc cccttcccgg cctcgctctc cacccggagc catcctcccg cggccatcgc
       61 ccgctggcgc atcatggcga tccccatgtg gctgggcgac gggacgacgc cctcgggctc
      121 gaagccgacc ccgtcgtcct cgatccggac gaagaggcca tcgtcggact ccaccgccac
      181 gtccacccgt gtggcccggg cgtgcttctg gatgttcgcc agcgcctcct ggaggatccg
      241 gtaggcggcg gaggccgtct cgaagggcgg ctcctcgatc acccgctcgc tgagccgggc
      301 ttcgaggtcc gcgtcctcgg ccgcccgctc cagcatggcc cgcagctcgg cgaggagccc
      361 gaactcctcc cgcggccccc tggccagctc gaacgtgagg cctcgcaggt gctcgatcgc
      421 ggcggtgagc gtctcctgga tccctccgat ctccgaacgg agggcagggt cctcctgcgc
      481 ctggcgcagc cgctggagcc gcgtcgagat cgtggagagc acctggagcg gctcgtcgtg
      541 gatctcggcg gcgatccgct ggcgctcgag gtcgcgggcc ttcaccagcc ggctgagcag
      601 ccggcgccgc gctacgagtc gcgccgagta cagcgagtcg atcggatccg tccctgcgtc
      661 caccgatgtg gtcacgccat gcccccttgc tctcccgacg cggtccccgc accttgtatc
      721 ggcgggggcg gcggggaaac ttgatgtcgc ggatcgcccc accgcggtcg gatgggacct
      781 gggtcggtcg gcggaccgcc cagcctcggc gtccctgcta ccctcggccc atccccatgg
      841 gcaccaggag ccgcgctcgc ctcgccacga ccgcgatcct cgcgtccttc ggcgcggtgg
      901 cggtcctggc ctcccggccg aacgctccct tcacgccgtt cctccacccg ggcgaccgat
      961 ctccgctgcc cctccgggag ctctcccgcc tcctcggact cgacgcccta gaccgggacg
     1021 tcgcggccct cgtcggggcg gccctgctcg tggcctcggc gatcgccttc gtgtatgcgg
     1081 cccgggtcgc ctggagggga gacctctcgg tgtcgagggt gatctgggtg ggggtggcgc
     1141 tccacgtcct ggcgttcgtg ctgccgctct tcctgtcccg cgacgtctac agctacgcgt
     1201 actacggccg gatggtgtcg cggtacggcg tgaaccccta cctgttcacg cccggccagt
     1261 tcctgcagga tcccttctac ctgtacgtgt cgcacttctg gatcgactcc ccctccgtgt
     1321 acggacccgc gttcagcgcc ctcgccggct gggcgacggg catcgcctcg agcgtcccgg
     1381 ggacggtgga tgccttcaag ctcgtggcgg ccgtggcggg tgtggggacg atgctggccg
     1441 tcgtggccgc cgcccggcag gtccggcccc ggcgggccgc cttcgccggc ctcctgatcg
     1501 gatggaaccc cgtgctgatc ttccacaacg tcgccggggg gcacaacgac gcgctcgtgg
     1561 gcctggctgt ggccctggcc gcgcttctcg tgctgtcccg tcgagacgtg ctggccacag
     1621 gggtcctcgt cctgggatgc ctggtgaagg cgacggcggt gatcccgctg gcgatgctcg
     1681 tcgtgggctc cgtggcggcc cggccgaagg gtcggcggct gcggtcgctg gcggcacacg
     1741 cggctatggg tctggtggtg ggacttccgt tcgtgatccg cttcttctcg ctggaggacc
     1801 cctcgatggg ccagctcgag ctcgccacgc gacagggttg gctggccccg tcccgtttcc
     1861 tcgtggtcac cctccgggag gcggtgaacg ccgtggccgg cgagaccgcg ggagatctca
     1921 cctcggcgct catccgaatc gtctttcccg tggtcctggc gctcgtcctg gtctcgatcg
     1981 ggaggcacgt ggctcgacgg cccgacagga tccgacccga gctgatcgtt gcggcgatgg
     2041 ggtgggccac gctgttcggc ctgatgatgg ccccgatcct gctgccctgg tacgcggcgt
     2101 ggatgatccc gctggcgtgg ctcctgccca agcccgcccg aggcggggcc atcttcatct
     2161 cggtggcgct cgtgatcacc gagttgatga gcgagcccac gaggtcacct cgcgtgtggg
     2221 aggtgatggt gatctggctc cactgggtgg ccacgccgat cgtgctcgcg gtgctggtgc
     2281 ggctgctgct ggagctccgg gcccgcctcc ggatggggcc cgcggagggc gcgtccgacc
     2341 cgctcctgac cgagcggccg atcctacgct cgcgggtgct gcggatcctc gaccgggcgg
     2401 tgtcgggccg cgaggacgta gccgacgagg gcgagcgtcc cggcgaaggc ggcgccgcct
     2461 cttcccgcgg ggagcgagag ccggtcggcg gctagcccgc ccaggatcag cacgcccagg
     2521 cccgctgcgg gggcggcccc cagagcggcc aggagcccgg cgcccgtgag cccccaggcc
     2581 gaccacccca agccgaggag cgccaggacg agcgcggcga gcagcgagag gcccacccac
     2641 gaccaggggg agatcccggg gtcgggccct gaggggtcgg cggccggcgc ggtgaacccc
     2701 tcgggggcgc ggaggaggac ggcacggggc gagaggggct cgccgagccg ccgggcgtcg
     2761 gcgaacccgg ggtggaacgg ctcgaggacg aggatcgcgg cctcgtcgag caccgaacga
     2821 gcctgctcca gggaggcctc cgcgattccc acgtactcgg ggtcctcgct ccgggtgccg
     2881 ggccggccgg ccaggacgtc ctcggcgtcg cccacgacga ggtgtgtctc ggctatccgc
     2941 tcggccggga gggcggcccg gatcacgttg ccgaagcgag cgacgtggaa ggcggcggcg
     3001 agctcctccg tgtccaccgc cacgacgacg ggcgtgccct cggggagcgc ttcgaggacg
     3061 ggctggaccg acccgacgct gcgcagctcc tcctcgctca tgaagggggt ctgccggtac
     3121 cagccccaga gcgaggaggc tgccacgatg gcggcggccg cggcggcggc gatccggatc
     3181 cacccgcc
//
LOCUS       DASUTW010000292         3335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_484589, whole genome shotgun sequence.
ACCESSION   DASUTW010000292 DASUTW010000000
VERSION     DASUTW010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484589"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VF058_10025"
     CDS             <1..764
                     /locus_tag="VF058_10025"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6208677.1"
                     /translation="IAAATTFPRREPHAGTTGRRPTATLLALGLVGGSIPFVLFFEGL
                     ARASSTQAAFIHKTLLLWVAALAVPLLRERVGLPHLGAFALLIAGQAALTGGSGGFGW
                     GAGESMVLAATLLWAVELVIAKRLLRSFGSSTLAVARMGVGSVALLGWLAVTDRLELL
                     LGLGASQWAWALGTGAILAAFVGTWFAALARAQAVDVTAVLVFGAVVTGVLASGVQGA
                     PLASQAPGLLLVTVGTAIIAATALRPVPAPARETT"
     gene            761..1522
                     /locus_tag="VF058_10030"
     CDS             761..1522
                     /locus_tag="VF058_10030"
                     /inference="COORDINATES: protein motif:HMM:NF040399.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6390 family protein"
                     /protein_id="HEX6208678.1"
                     /translation="MTLGGSLLFARYAFPPNALGYCGPDASPTMAEHLAQGHADPDLE
                     RLARGFEGAWPYLRLIAESSGIGDPLDERVVEAYWVGGPLLDRVRVSALGRFLGETFR
                     GRAGSRWEDLEALAGAGGVPHHNLHVFGVYPWMGLLRMGHETEPMRVLEACRTRWARV
                     LAVEPGRALVRSRTLAWNGRRLALGHPGVSWATTEAGGREVAPRLAPGDLVAVHWDRV
                     CDRLTRRRARTLHRETLRALAAAQRSSVGPAAVLS"
     gene            complement(1525..2232)
                     /gene="otsB"
                     /locus_tag="VF058_10035"
     CDS             complement(1525..2232)
                     /gene="otsB"
                     /locus_tag="VF058_10035"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX6208679.1"
                     /translation="MPGDLPRLRRGPDPDRPGPDQAILDDEMRGALLRLRDRWPVAVV
                     SGRDLQDVRGRVGVDGVAYAGSHGFEIVTPGGRQELKGEDYLPDLDAAEGKLREALPG
                     GARLERKRAAIAVHVRGMEADEIPGVERVVDEVAARHTRLRVTGGKRIFELRPDLDWD
                     KGRALRWFLEVLGLTDAVPVYVGDDLTDEDAFQEIREDGLGVVVRGEHDERPTLAHAA
                     LDGPDEVRGFLEALADL"
     gene            complement(2324..2521)
                     /locus_tag="VF058_10040"
     CDS             complement(2324..2521)
                     /locus_tag="VF058_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208680.1"
                     /translation="MAEFQLAGRLVQGTRRPSRRYRRGRVCAHNGCDTVLSVYNPARF
                     CAQHEATQLPRTFGRQRLRSA"
     gene            complement(2609..>3335)
                     /locus_tag="VF058_10045"
     CDS             complement(2609..>3335)
                     /locus_tag="VF058_10045"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX6208681.1"
                     /translation="DEAAALLGEIVAAGPRWLEPDETFRLLDCYGIPLASWRLASTPE
                     EAGAAAGELGGEVALKAVAPGLIHKTEAGAVRLSLEGSEAVRGAAEEMAASVKEAGHE
                     VEGFLVQAMVSGGIEMLVGVVHDALFGPVVACGAGGTTAELVKDVAVRITPLTDLDAS
                     EMIRSLQTFPLLEGYRGAPGGDVGALEDLLLRVGAMVEDNPAIAEMDLNPVIVLPEGL
                     AVVDARVRAEIPAPSRPLSARRR"
BASE COUNT          385 a         1287 c         1204 g          459 t
ORIGIN      
        1 tgatcgccgc cgcgacgacg ttcccccgcc gcgagcccca cgcggggacg acgggccgcc
       61 gaccgacggc gaccctcctc gcgctggggc tggtcggcgg cagcatcccc ttcgtgctct
      121 tcttcgaggg cctggcccgc gcctcgtcca cgcaggcggc cttcatccac aagaccctgc
      181 tcctgtgggt cgcggccctc gcggtcccgc tgctccgcga gcgggtgggg ctcccgcacc
      241 tgggggcgtt tgccctgctg atcgcgggcc aggccgcgct caccggcggt tcgggaggct
      301 tcgggtgggg cgcgggcgag tcgatggtgc tcgccgccac gctcctgtgg gccgtcgagc
      361 tcgtgatcgc caagcgtctg ctccgctcct tcgggtcctc cacgctggcc gtcgcgcgga
      421 tgggcgtggg ctccgtcgcg ctcctcgggt ggctcgccgt gacggaccgc ctggaactcc
      481 tgctggggct cggggcgagc cagtgggcgt gggctctggg caccggcgcc atcctggcgg
      541 cgttcgtggg gacctggttc gcggcgctgg cgcgagccca ggccgtcgac gtcaccgccg
      601 tcctcgtctt cggcgcggtc gtcaccggcg tgctggcctc gggggtacag ggcgccccgc
      661 tcgcctccca ggcccccggt ctgctcctgg tgacggtggg caccgcgatc atcgccgcga
      721 ccgccctgcg gccggttccg gcccccgccc gggagacgac gtgaccctcg gcggctcgct
      781 gctcttcgcc cggtacgcct tcccccccaa cgcgctcggg tactgcggcc ccgacgcatc
      841 gcccacgatg gccgagcacc tggcccaggg gcacgcggac cccgacctcg agcgtctggc
      901 ccgcggcttc gagggggcct ggccctacct gcgcctgatc gccgagtcga gcgggatcgg
      961 ggacccgctc gacgaacgcg tggtcgaggc ctactgggtc ggagggcccc tcctcgatcg
     1021 ggtgagggtg agcgctctcg gccgcttcct gggcgagacg ttccgaggcc gggcggggag
     1081 ccggtgggag gacctcgagg cgctggccgg ggcggggggc gtcccgcacc acaacctgca
     1141 cgtgttcggc gtctacccgt ggatggggct gctccgcatg ggacacgaaa ccgagcccat
     1201 gcgggtcctc gaggcctgtc ggacacgatg ggcccgggtg ctggcggtgg agcccggacg
     1261 ggcgctcgtc cgctcacgga ccttggcctg gaacggacgg aggctcgcgc tcggccatcc
     1321 cggggtgagc tgggcgacga cggaggcagg agggagagag gtggcgcctc gcctcgctcc
     1381 cggcgatctg gtcgcggtgc actgggatcg ggtctgcgac cgcctcacgc ggcggagagc
     1441 acgcaccctg caccgggaaa ccctccgcgc gctcgccgcc gctcagcgga gctccgtcgg
     1501 ccccgcggcc gtcctgagct gacctcagag atccgccagc gcctccagga agccgcggac
     1561 ctcgtcgggg ccgtccaggg cggcgtgggc cagggtcggc cgctcgtcgt gctccccgcg
     1621 cacgacgacg cccaggccgt cctcgcggat ctcctggaac gcgtcctcgt cggtgaggtc
     1681 gtcccccacg tagacgggga cggcgtccgt cagccccagc acctcgagga accagcggag
     1741 ggctcggccc ttgtcccagt ccaggtccgg acggagttcg aagatccgct tcccgcccgt
     1801 gaccctgagg cgcgtgtgcc gggcggccac ctcgtccacg acccgctcca cgcccgggat
     1861 ctcgtcggcc tccatccccc ggacgtgcac ggcgatcgcc gcccgcttcc gctcgagccg
     1921 ggccccgccc gggagcgcct cccggagctt tccctcggcc gcgtcgaggt cgggcaggta
     1981 gtcctctccc ttcagctcct ggcggccccc cggggtgacg atctcgaacc cgtggctccc
     2041 cgcgtacgcc acgccgtcca cgcccacgcg cccgcgcacg tcctgaagat cccgcccgct
     2101 gaccacggcg accggccacc ggtcccgcag acgcagcagc gcgccccgca tctcgtcgtc
     2161 gaggatcgcc tgatcggggc cgggacgatc ggggtcagga ccccgtcgta gtcgaggaag
     2221 atcgccgggg atcgtccggc caggtcctcg agacgctccc tcgcgtcggg cagatcgcgg
     2281 atcggcacgc cctcgctcac gccgcccgcc ggtgtggggg gggtcaggcg gagcgcaggc
     2341 gctggcgtcc gaacgtgcgg ggcagctgcg tcgcctcgtg ctgggcgcag aacctcgcgg
     2401 ggttgtagac ggagagcacc gtatcgcagc cgttgtgcgc gcagacccgc cctctccggt
     2461 agcggcggct ggggcgtcgc gtcccctgaa cgagcctccc cgccagctgg aactcggcca
     2521 tgtgatccct ccgacacgga gcttctcagt ccggcggcct ccgtccgagg gccgagggtc
     2581 ccgcagccag agggaccgtc ggaccgggtc accgccgacg cgccgagagg ggcctgctgg
     2641 gggccgggat ctcggcccgg accctcgcgt ccacgacggc cagcccctcc ggcagcacga
     2701 tcacggggtt gaggtccatc tcggcgatcg cggggttgtc ctcgaccatg gccccgaccc
     2761 ggaggaggag atcctcgagc gcgccgacgt cgccgccggg cgcgccccgg tagccctcga
     2821 ggagcgggaa ggtctggagc gaacggatca tctcggacgc gtcgaggtcc gtcagcggcg
     2881 tgatcctgac ggcgacgtcc ttcaccagct cggccgtggt ccctccggcc ccgcaggcca
     2941 cgaccggccc gaacagcgcg tcgtggacca ccccgaccag catctcgatg ccgccggaga
     3001 ccatcgcttg gaccaggaac ccctcgacct cgtggccggc ctccttcaca gaggccgcca
     3061 tctcctcggc ggcgccgcgg acggcctccg acccctccag cgagagccgc acggccccgg
     3121 cctcggtctt gtggatcagc ccgggggcca ccgccttgag ggcgacctcg cccccgagct
     3181 ccccggccgc cgcgccggcc tcctccggcg tcgaggccag ccgccaggag gccagcggga
     3241 tcccgtagca gtcgaggagg cggaaggtct cgtcgggctc gagccagcgc gggccggcgg
     3301 ccacgatctc gccgaggagc gccgccgcct cgtcg
//
LOCUS       DASUTW010000293         2335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1132073, whole genome shotgun sequence.
ACCESSION   DASUTW010000293 DASUTW010000000
VERSION     DASUTW010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132073"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1629)
                     /locus_tag="VF058_10050"
     CDS             complement(67..1629)
                     /locus_tag="VF058_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208682.1"
                     /translation="MSSRRSWSVVIALVLSVGIAAPAHADGFHTALPPMLSLTGDAPP
                     GSSVVAIISSGDTIGDFTFEGIPDGIGLMPGAPGEVEVFVSHEQSTVPFLGFADFEDA
                     SVSQLTLDTSDAGVLEASVPIGPENGYIRFCSSFMAGPAEGFKTYTFFANEESSDIID
                     VPPGAPYGPDPAVAPQRQAGYAVVLNAETGAFTQVAGMGRHNHENSVVIPGGWGDRGK
                     GKKKGRAGKGAGPQIAILSTDDTFSPPTSQLYLYLANRESHIWQDKGSLWGFQATAKN
                     GSRVNPRNAFNGANDYLDLDPGDEIKGRFIRVPKRIARGKTALPPQEALERWSNRHNV
                     FQFIRLEDLAYDRHNPRVVYIADTGASRVVPDPATGRMQRGPSGTVGEADNGRIFRMV
                     MNKNNPRKVNSLTVYADGDWEGTDVFVPMRAPDNIDTSGASLMVQEDADDAKVWAHDF
                     ASGSWTHVATVDDPDGESSGIVDASAFFGDGAWLLDVQAHGSFESSEVIDGVTYKREN
                     GQLLLMILPGTT"
     gene            complement(1718..>2335)
                     /locus_tag="VF058_10055"
     CDS             complement(1718..>2335)
                     /locus_tag="VF058_10055"
                     /inference="COORDINATES: protein motif:HMM:NF042466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HEX6208683.1"
                     /translation="MLHTLGQEELERSLFPIGEQTKDETRAHARRLGLPVAGKPDSQE
                     LCFVPRADGGAFLEERAPHLVREGEMVDADGLVVGTHAGAHRFTIGQRRGLGVSLGER
                     AYVVDVDAAANRVVVGPGELLARKGLLAESVHWVGTPPAGPIEAEAQIRYNAPAVPSV
                     VEPLPGDDVRVTFRAPQRAIAPGQSVVFYAADELLGGGTIARSLR"
BASE COUNT          339 a          816 c          822 g          358 t
ORIGIN      
        1 agaaggagcg gggggggccg gccccgtcgg ggccggcgcc ccccgccaca cgctcgacga
       61 ccccgcctag gtcgttcccg gcaggatcat cagcaggagc tgaccgttct cccgcttgta
      121 ggtgacgccg tcgatcacct ccgacgactc gaaggagccg tgggcctgaa cgtccagcag
      181 ccaggccccg tccccgaaga acgcggaggc gtccacgatc ccgctggact ccccgtcggg
      241 atcgtccacc gtcgccacgt gcgtccagga tccggacgcg aagtcgtggg cccagacctt
      301 cgcgtcgtcg gcgtcctcct gcaccatcag ggacgccccg ctggtgtcga tgttgtcggg
      361 cgcccgcatc gggacgaaga cgtcggtgcc ctcccagtcg ccgtccgcgt acacggtcag
      421 ggagttcacc ttccgcgggt tgttcttgtt catgaccatc ctgaagatgc ggccgttgtc
      481 ggcctcgccc acggtgccgc tcgggccccg ctgcatccgg ccggtcgccg ggtcgggcac
      541 gacccgcgag gctcccgtgt cggcgatgta caccacgcgg gggttgtggc ggtcgtaggc
      601 caggtcctcc aggcggatga actggaagac gttgtgacgg ttcgaccagc gttcgagcgc
      661 ctcctgcggc ggaagcgccg tcttcccacg cgcgatccgc ttcggcacgc ggatgaaccg
      721 acccttgatc tcgtcgccgg ggtcgaggtc caggtagtcg ttcgccccgt tgaaggcgtt
      781 cctggggttc acgcggctcc cgttcttggc cgtggcctgg aacccccaga ggctcccctt
      841 gtcctgccag atgtggctct cgcggttggc caggtacagg tagagctggg acgtgggcgg
      901 gctgaaggtg tcgtcggtgg acaggatcgc gatctgcggc ccggcgccct tcccggcgcg
      961 ccccttcttc ttgcccttgc cccggtcgcc ccagccgccg gggatcacga cgctgttctc
     1021 gtggttgtgg cgtcccatgc cggcgacctg ggtgaaggcc ccggtctcgg cgttcaggac
     1081 gaccgcgtag ccggcctggc gctggggggc gacggcggga tccggaccgt agggggcgcc
     1141 cggcggcacg tcgatgatgt cgctcgactc ctcgttggcg aagaacgtgt aggtcttgaa
     1201 gccctcggcg gggcccgcca tgaacgagga gcagaagcgg atgtagccgt tctcgggccc
     1261 gatcgggacc gaggcctcca agacgccggc gtcgctcgtg tcgagggtga gctgggacac
     1321 cgaggcgtcc tcgaagtcgg cgaacccgag gaacggaacc gtggactgct cgtgggagac
     1381 gaagacttcg acctcgcccg gcgctccggg catcaggccg atcccgtccg ggatcccctc
     1441 gaaggtgaag tcgccgatcg tgtcgccgct cgagatgatc gcgacgaccg acgagccggg
     1501 cggagcatca cccgtgaggg agagcatcgg tgggagcgcg gtgtggaacc catccgcgtg
     1561 agccggagcg gcgatcccca cggacagcac cagcgcgata acgacggacc atgagcgtct
     1621 ggagctcata cgtgcctcct tccgctgggt tttggcccga cattgtgaca cccatgagca
     1681 agccggagtc aactccgggg gaacggctcg atggacctca gcggagcgag cgggcgatcg
     1741 tgccgccgcc gagcagctcg tcggccgcgt agaacacaac gctctgcccg ggggcgatcg
     1801 cccgctgggg agcgcggaag gtgacgcgga catcgtctcc cgggagcggc tcgacgaccg
     1861 aggggaccgc cggggcgttg tagcggatct gagcctcggc ctcgatcggg ccagccggcg
     1921 gggttccgac ccaatggacg gactccgcca ggaggccctt cctggccagc agctcgcccg
     1981 ggccgaccac gacgcggttc gccgcggcgt cgacgtccac gacgtaggcc cgctcaccga
     2041 gcgagacgcc cagcccgcgc cgctggccga tcgtgaagcg gtgcgccccg gcgtgcgttc
     2101 cgacgaccag accgtcggca tccaccatct cgccctcccg gacgaggtga ggggcccgct
     2161 cctcgaggaa cgcgccaccg tccgccctgg gcacaaagca cagctcctgc gagtcgggct
     2221 tccccgccac ggggaggccg agccgccgag cgtgagcgcg cgtctcatcc ttcgtctgct
     2281 ccccgatcgg gaagagggac cgctcgagct cctcctgccc cagcgtgtgc agcat
//
LOCUS       DASUTW010000294        12486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_225126, whole genome shotgun sequence.
ACCESSION   DASUTW010000294 DASUTW010000000
VERSION     DASUTW010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12486
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225126"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            561..956
                     /locus_tag="VF058_10060"
     CDS             561..956
                     /locus_tag="VF058_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX6208684.1"
                     /translation="MWAEANERIELVSAKKLAEEIVSGGILLLDVRLPIEIERRGTIA
                     GSVPVPRQVVEWWADPDSPYFRPGGIFGDFEKRVVTFCDGGGNGAFTAVALQELGYRN
                     VATLEGGFYGWVGAGLPVVDASDRPSTGT"
     gene            1252..4641
                     /locus_tag="VF058_10065"
     CDS             1252..4641
                     /locus_tag="VF058_10065"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6208685.1"
                     /translation="MSSRAFETSASSTQPSRAGHPPRGAFLGRERELAELLAGLDDVL
                     SGRGRLFLIGGEPGIGKSRLADELAAQARSRGATVLWGRCWEAGGAPAYWPWVQCLRS
                     YLRTHDEATVRDHMGAGAADMAQMLPEVEALVPDLPPPASVDPESARFRLFDSTVTLL
                     RNAGDAEVLVIVIDDLHAADTASLLLLRFLAGQLGETRVLLLATYRDVELTPDHPLTA
                     AIPELTREPTTHHLLLRGLDKPDVARLLHDAAEVTPPPALLSEVHRRTNGNPLFVGET
                     ITLLAAEGGFTPDAEAAPPRFTIPGAVRDVILRRLGHLSEPCRRALTLASVLGTEVTI
                     EALRQLGDISADEFPGIEDEAAASGLLVGLPGSPGRVRFSHGLVREALYEEVPPARRA
                     RLHLRAGELLERMYSGRTDPHIAEIAYHFFEGASAGEPGKAVDYATRAGDRAAGSLAY
                     EEAARLYRMALQALEVAPSEDEEARGDLLLALGDAQARSGDLSSARETFLRASDIARR
                     TGAAHQLGRAALGYGGRFVWARAGDDPHLVPLLQDALVLLGGSDDRMRVRLLTRLACA
                     LRDSPDRERSASLSKEAVDLARTLDDSASLAYSLEGRIWATWWPENPQERLEIAAELL
                     RLAEETREGELIVDGHVARWAALTELGDMGAARAEAEALSRAADELRQPAQRWVGNAT
                     RIMMLQLEGDFGAAEGLIQEALEARQSTPVRDNLSAARFQMFLLRREQGRVGETEAMV
                     RESAREFFWYPVHRTALVSLLLDVGRVGEARVAFEELARDGFRALPRDNYWLLGTSLA
                     AEACSMLGDEEAAAVLYEQLLPFQGRPAIGPAEGTVGALDRYLGLLAVTLGRPEDAER
                     HFEEAIQVNGRMGARPWTAHSQCDYARMLLSRGGPGDRERAEGLLSEALATCRTLGMA
                     ALERKVTELLGESPPSARAPAGPAEPCVFRREGEYWSVAFEGKAFRLRDLKGLRYLAR
                     LLAEPGREFHVLDLVATERGAAPATTRAEPGLGAPGDTGEVLDAKARAAYRDRVAALE
                     EEIEEARSFGDTERAARAEKERDFLVGELAGAVGLGGRPRRAGSEVERARVSVTQAIR
                     TALRRIREQSSALGAHLDRTVLTGTFCSYVPDPRAPVDWRT"
     gene            4781..5242
                     /locus_tag="VF058_10070"
     CDS             4781..5242
                     /locus_tag="VF058_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208686.1"
                     /translation="MATIAIQPQAARRSGPWWVLFLIAAAGIAGVSLVLVRTMTSEGE
                     VAGRTAAFSSVERATHTRALNQPEAASQIAGSTSFTFNAVQDLRALNEAEAATGGLDL
                     AAVAEVRAANTPSSPAHSPSSSEGIRHARALNEPSSKLASSVEAVTDPRWR"
     gene            5981..9241
                     /locus_tag="VF058_10075"
     CDS             5981..9241
                     /locus_tag="VF058_10075"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX6208687.1"
                     /translation="MADELDQEEDMDFRILGSFEVASNGEVADLGPPKQRTLLAVLLL
                     HANNLVPTDQLIELVWGDRPPRTAAHSIQIYVSELRKALEPMAGPPLILTRPPGYVLK
                     TDPDSIDARRFEGLVAAGAHELDAGDHPRARATLREALGLWRGRPLSDFAYEEFAQPE
                     IRRLESLRLDALEQLAATELASGREQEVLPLIEAVIRDDPRRERSRELQMLALYRTGR
                     HPEALRVFQEFRGLLADELGLDPSPRLRRLQEQILLHDPSLEPPAQPGPTPAGPHLRN
                     PYKGLQPFGEEDAKDFFGREDLVRRLLDALKGGIRLLALVGPSGCGKSSVVGAGLVPA
                     LRRGALPGSDRWMIVRMLPGARPSEELEAALLRVAGEPGARLEPLDGGDDGLLRAARE
                     LAPRVGPLVLVIDQFEELFSLSDEPSRAAFLRNLATAVGDPGGPVVILTLRGDFYDRP
                     LLHPEFAAHFTRGVVNVVPMTATEIEAAVVGPARRVGVEVDPALLAELIAETVDRPAG
                     LPLLQYALTELFVRRSESTLSLEEYRAVGGVRAALSRRAEELYGSLDEDRRWVAMQVF
                     LRLVRLGEGTRDARRRVPVGELTALDLDPVALSEVLKAFGRHRLLSFDRDPAGGGATV
                     EVAHEALLSEWERLAEWIEAHRGDLRRHGSLAVAVEEWESSGRHPDYLLTGNRLAELD
                     GWVRSTALELTESERRFPAAGLERLREEQAGEAERLEQQRRLERRARTRLWALAAALV
                     LLVGGATYGVLAFLGGGPPDAVLVFEGPGDGSFNDMADAAFDRAAAELGLDVDKVTGS
                     RASLESVLRRVSEQDVGLVVLALPAFPDDVEPVARDHPDTLYVLNSEGRGPDVIPNAI
                     YMNFANAEGSFLIGAAAALKSETGTIGFIGGLDFPFIWQFQAGYEAGARAVDPGIEVQ
                     AEYLARLPDESGGFDSPTLAAQVADRQFRAGADVIYHAAGRSGLGLFDTAEALSEELG
                     RHLWAIGVDADQYRSVVAGEAHPAWEPEAWPPHILTSMLKRYDNALFSILEDYSRGML
                     TPGGRELNLASGGVDISYTGGFIDDIRPELERLRQQIIEGDIEVPTIPAEKAGADF"
     gene            9347..9682
                     /locus_tag="VF058_10080"
     CDS             9347..9682
                     /locus_tag="VF058_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208688.1"
                     /translation="MKLAPTREHPAPVAPGEVVLAPPQRRRGLSRRLVLVTVVLALVA
                     GGVGGYVAYLVSEDGLTRGRATDAARWEAMGDAHLRDQVALARGRAAEAARWQALADA
                     IREGTLAGP"
     gene            9848..10024
                     /locus_tag="VF058_10085"
     CDS             9848..10024
                     /locus_tag="VF058_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208689.1"
                     /translation="MDGLLATLEQDDLLEEALAEWRGLAEESLPRRETELARIEARIR
                     KATNPLDRHFLAFE"
     gene            complement(10318..10392)
                     /locus_tag="VF058_10090"
     tRNA            complement(10318..10392)
                     /locus_tag="VF058_10090"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10357..10359),aa:Arg,seq:ccg)
     gene            10454..10828
                     /locus_tag="VF058_10095"
     CDS             10454..10828
                     /locus_tag="VF058_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6208690.1"
                     /translation="MASGRILIADDDPVILRLLEVNFSLEGFDVETAERGERVLEEAR
                     RVRPDVIVLDVMMPGMDGWEVCERLKEDPGTADIPIMVLSARTQEQDRERGRELGVSA
                     YVTKPFDPEELIRVARSLLPEE"
     gene            10842..>12486
                     /gene="argS"
                     /locus_tag="VF058_10100"
     CDS             10842..>12486
                     /gene="argS"
                     /locus_tag="VF058_10100"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX6208691.1"
                     /translation="MSEAEVLSLIRRALEDAQGELGLAEIPEPIEVVRPRQREHGDYA
                     TNVALAVGAALGRNPREVAEVVVRHLPESDLVTKAEVAGPGFINFTLAHVWLHRVLHE
                     VERQGEAYGRVDEGGGERVQVEFVSANPTGPLHVGTGRNAAVGDALASLLDAAGHRVE
                     REYYVNDAGTQITLFAASIEARYLERFGHEAKVPEGGYEGAYVRELAERIAEDVGEEL
                     LDLPQEERLARFREEGIRRSLDGIRATLERFGIRFDTWFPESQLVESGRIHQAMELLR
                     ERGFAYEDEGALWFEASRYGDEKDRVLIRSTGEHTYFAADVAYLREKFARGFDRLVYV
                     WGADHHGAVKRLRGAAQALGYDPEAAEFILLQLVALYRSGEPVKMSKRTGEIITLDEL
                     LDDVGPDAARYTLLTRSPDTALDFDIDLVASQSLDNPVYYVQYAHARIASILRFAEER
                     GVGMRPLDEVDLDLLQDETELALLRKIAELPEQVALAANLRAPHRLTRYVETVAAGFH
                     RFYTACRVVTDDEELTQARLHLAGAARRVIANVLAIIGVS"
BASE COUNT         1853 a         4148 c         4526 g         1959 t
ORIGIN      
        1 ctcgccggcg cgcagcaacg gtcgagcttc gatgacgtgc cccctcctct gccccggagc
       61 gctggcccgc gctcggatat gcacgttcgc atctcggggc tcctcgcgcc cgggtcgtcc
      121 ggaccgctgg gccagggacc atcgggctcg gcggtctgcc tctgtaggtg cgaccaacct
      181 gatccagtga cggtcagacc agcagccaaa aaggcggcgg aggcgcgaat gagaggccgt
      241 cgacgatgtc tcggtgttgt ccccgaccag gcacctttgc gtacacccaa ccgtacagaa
      301 gtcgagaagt ggtccgcagg tggagggcct acgagtcgtg ttgagcaagc gccatgggaa
      361 gattcggcgc gacggctaca ggcgtccgcg ctgactccga ttctccattc acaaggcaac
      421 cgagctcagt actgccgtcc ctggccgtcg atactgagcg gctggtggcg gaaccccgcg
      481 gcgtcgccgc gattcgtgta gtactaagat tcggggtact gaccatgtag gtggtagtgg
      541 cgcgaagatc ggtgctgcag atgtgggccg aagccaacga gcggattgag ttggtgtcgg
      601 ccaagaagct cgccgaggag atcgtctccg gtgggattct gcttctggat gttcgtctgc
      661 ccatcgagat cgagcggcgg gggacaattg ccggatcagt tcctgtgccg cggcaagtgg
      721 tggagtggtg ggcggatcca gatagtccgt attttcgacc tggcggcatc ttcggggatt
      781 tcgaaaagag ggtggtcacg ttctgcgacg gtggtggaaa cggtgccttc accgccgtgg
      841 cgctgcagga actcggatat cgtaatgtag ccacgctcga aggcggattc tacggatggg
      901 tcggtgctgg cctgcccgtc gtcgatgcct ccgacagacc ctctaccggt acctagctga
      961 cacggcggtt ccggatacat cgaccgatgt tccaccggat cgttcttcat ccgccggagt
     1021 tggcgcccat gacagccgcg cagcgaaaag ccgcggtccg aagcctggcg cttctccttg
     1081 acgcctcgat gcaggcgaac aggagcccgg gatcggagag aggcgcttgc ggaagtgcag
     1141 ctccgcggcg tggcggtttc caagaggcga gggaggtctg atgacctgac gtcgcaccct
     1201 cgatccaatt ggtgctggac accggagcgc gctccgagaa aggatgagcg ggtgagctcc
     1261 cgagcgtttg agacgtcagc gtcctccacg cagccgtctc gcgccggtca tcccccccgg
     1321 ggtgccttcc tgggccgcga gcgggaactg gccgagttgc tggccggtct ggacgatgtt
     1381 ctctcgggac gaggacgact gttcctcatc ggcggggagc ccgggatcgg caagagccgt
     1441 ctggccgacg agctggccgc gcaggcgcga agccggggcg ccaccgtgct ctggggacgc
     1501 tgctgggagg ccggcggagc ccccgcctac tggccttggg tccagtgcct gcgctcctac
     1561 ctccggacgc acgacgaagc gaccgtccgc gaccacatgg gggccggcgc cgccgatatg
     1621 gcacaaatgc tgccggaggt cgaagcgctc gtccccgatc tcccaccccc cgcgtccgtc
     1681 gatcccgaat ccgcccggtt ccggctgttc gactccacgg tcacgcttct tcgaaacgcc
     1741 ggggatgcgg aagttctggt gatcgtgatc gacgacctcc acgctgcgga cacggcttcg
     1801 ctccttcttc tgcggttcct tgccggccag ctgggggaga cgagagtcct gctcctggcg
     1861 acgtatcggg atgtcgagct cacgccggac catccactga ccgccgccat cccggagctg
     1921 acgcgagagc ccaccacgca tcacctcctc ctccggggcc tggacaagcc agacgtcgcg
     1981 cggctcctcc acgacgccgc ggaggtgacc cctccaccag ccctcctctc ggaggtgcac
     2041 cgccggacga acggcaaccc gctgttcgtg ggcgagacga tcaccttgct ggcagcggag
     2101 ggtgggttca cccctgacgc cgaggccgcg cccccgcgct tcacgatccc gggtgcggtt
     2161 cgagacgtca tcctgcgccg ccttggtcac ctcagcgagc cttgccggcg agccctgacg
     2221 ctcgcctccg tcctgggcac cgaggtcacg atcgaagccc tgcgccagct cggggatatc
     2281 tcggcggacg agttccctgg aatcgaggac gaggcggccg catcgggcct cctcgtaggg
     2341 cttccggggt ctcccggccg cgtgcgcttc tcccacggcc tcgtccgaga ggccctgtac
     2401 gaggaggtcc cgccggcccg gcgagcccgc ctccacctgc gagcgggaga gctgctggag
     2461 cggatgtact ctggccgcac cgacccgcat atcgccgaga tcgcctacca cttcttcgag
     2521 ggggcctccg caggtgagcc cgggaaggcg gtcgactacg cgacgcgggc gggagaccgt
     2581 gcggctggat cgctggccta cgaggaggcc gcccgcctct accggatggc ccttcaggcc
     2641 cttgaagtcg ccccctcaga ggatgaggaa gcgcgcggag atctcctcct cgcgctgggc
     2701 gatgctcagg cacgctcggg ggacctttcc tccgccaggg agacgttcct gcgtgcatct
     2761 gacatcgcaa ggagaacggg agcggcccac cagctggggc gcgcggcact cgggtacggc
     2821 ggccggttcg tctgggcccg tgccggcgac gacccgcacc tcgtcccatt gctccaagat
     2881 gccctggtcc tcctcggggg aagcgacgat cgcatgcggg tgcgcctgct gaccaggctc
     2941 gcctgcgcgc tccgcgactc ccccgaccgc gagcggagcg cctcgctgtc gaaagaggcc
     3001 gtggacctgg cccgaaccct cgacgactca gcgtcgctgg cttactcgct ggaggggcgc
     3061 atctgggcca cctggtggcc cgagaacccc caggagcgcc tcgagatcgc ggccgagctc
     3121 cttcggctgg cggaggagac tcgtgagggg gagctgatcg tggatgggca cgtggcgaga
     3181 tgggcagccc ttacggagct cggggacatg ggggcggcga gagcggaggc ggaagcgctg
     3241 tcgcgcgccg ccgacgagtt gcggcagccg gcgcagcgct gggtggggaa tgcgacccgg
     3301 atcatgatgc tgcaactcga aggcgatttc ggagcggccg agggtctcat ccaggaggct
     3361 ctggaggcgc gtcagtccac accggtccgc gacaacctgt cggcagcccg attccagatg
     3421 ttcctccttc gcagggaaca gggccgggtc ggcgagaccg aggccatggt ccgcgagtcg
     3481 gcgagggagt tcttctggta cccggtccac cggaccgcgc tcgtctctct tctgctggac
     3541 gtgggccggg tcggtgaggc gcgcgttgcg ttcgaagagc tggcccggga tggcttccgg
     3601 gcgttgcccc gagacaacta ttggctcctc gggaccagcc tggccgccga ggcgtgctcg
     3661 atgcttggcg acgaggaagc ggccgccgtc ctgtacgagc agcttctccc atttcagggt
     3721 cgtcccgcga tcggccccgc ggaggggacc gtgggggccc tcgaccggta tctgggcctg
     3781 ctggcggtga ccctcggtcg gcccgaggac gccgagcggc acttcgagga ggccatccag
     3841 gtgaacgggc gaatgggggc tcgaccttgg accgcccaca gtcagtgcga ctacgcgcgc
     3901 atgctcctct cccggggcgg gcccggcgat cgtgagagag ccgagggcct cctgtccgag
     3961 gccctcgcaa catgtcgaac cctcggtatg gccgcgctgg agcgcaaggt caccgagctg
     4021 ctcggtgagt ctcctccgtc ggctcgggca ccggccggcc ccgccgaacc gtgcgtcttc
     4081 cgccgggagg gggagtactg gtccgtggcc ttcgagggga aggccttccg cctccgagac
     4141 ctcaaaggcc tccgctacct ggcccggctg ctcgccgaac ccggccggga gttccacgtc
     4201 ctggaccttg tggccacaga gcggggagcc gcccccgcga ccacccgggc cgagccgggg
     4261 ctgggggcgc ccggggacac cggggaggtc ctcgatgcga aggcccgggc cgcctaccgg
     4321 gaccgggtgg cggcccttga ggaggaaatc gaggaggccc ggtcgttcgg ggacaccgag
     4381 cgggccgctc gggcggagaa ggagcgagac ttcctggtgg gagagttggc gggggcggtg
     4441 gggctcgggg ggcggccccg ccgtgcggga tcggaggtgg agcgggcccg ggtgagcgtc
     4501 acccaggcca tccgcaccgc cctgaggcgg atccgcgagc agtcttcggc cctcggggcc
     4561 cacctggacc ggaccgtgct caccgggacc ttctgctcct acgtccccga cccccgggcc
     4621 cccgtcgact ggcggacgtg acctgccggc gggtgctgcg gaagcgtaac gccccgcagg
     4681 cgtagttgta acgcccggtc tttctcccgt aagccccctc ctcacgcctc ttcttacgaa
     4741 aggccctcgc agggagggcc aaagaggcaa ggagatcgag atggcaacga tcgcaataca
     4801 gccccaggcg gcgcgtcgat cgggtccgtg gtgggtgctg ttcctgatcg ccgcggcggg
     4861 gatcgcgggg gtgtcgctgg tcctggtgcg gacgatgacc tcggaggggg aggttgcggg
     4921 acggacggcc gccttctcct ccgtggagag ggcgacccac acaagggcgc tgaaccagcc
     4981 cgaggcggcc tcgcagatcg ccggttccac ctcgttcacc ttcaatgcgg tgcaggacct
     5041 gcgggcactg aacgaggcgg aggcagcgac cggaggactg gatctcgccg cggtcgccga
     5101 ggtcagggcc gcgaacacgc cctccagccc ggcgcattcg ccaagctcct ccgagggaat
     5161 ccgccacgcc cgagccctga acgagccgag ctcaaagctg gcttcctcgg tggaagcggt
     5221 gacggatccg cggtggcggt gaaccgctga gtccccgatg cgccggaggg aggagggatc
     5281 aacccctcct ccctctcgcg cgtgggagtt ctggcccggg gatgggccgg atcctcgagg
     5341 aatcctcacc tccgggaggc tgcaatggca tttggcggac cggcctggat cacgagccgc
     5401 gttgatcaga gtctccaagg aatccggggc ggttcattat tggtcgcggg gttcatgcgt
     5461 tcctcccccg gaggagaccg ttgccgaagc ggtggctcgc ccgtccaaac catggtgggc
     5521 gcgttggtgg acccgcgggg gcggctccga gggcgatagg cgcttaggcc ccgcccccta
     5581 tgaccagatg gaggggggcc gctttcgcca caccgcccag ttcgtcctat ggcgacccga
     5641 ccgcgacgcg cgcttctgca cctacgagca gctccgtccc cgtcatccag actcggatga
     5701 aatcggctgc gcttgcttct gaccagcaac gatggacgca gcgagtcgac gttggactct
     5761 ctccgttgcc ttgccaaggt gggggcgcgg gttcgcgaat cccatcgtcc gctccaagtc
     5821 ctagcaggtc agtggcttgc gttcaggaaa caatcgtttg gggcccgaga atccagacgg
     5881 ttcgggcatc agtcattggg cccgacgctg acgctcctgg agcgcacgga cgggttcgta
     5941 catcgcagac acttggttcc tctgggacga acggctatcc ttggcggacg agctcgacca
     6001 ggaagaggac atggactttc ggatcctggg gtccttcgag gtggcctcca acggtgaggt
     6061 cgcggatctg gggcctccaa agcagcggac tctgcttgcc gtccttctcc tccacgcgaa
     6121 caacctcgtc ccaaccgatc agctgatcga gcttgtctgg ggggatcgcc cgccgcggac
     6181 cgcggcacac tcgatccaga tctacgtgtc cgagctgcgg aaggccctgg agcccatggc
     6241 cgggcccccc ctgatcctta cccgcccgcc cggatacgtg ctgaagaccg atcccgactc
     6301 gatcgatgcc cgccggttcg aggggctggt cgcggcaggc gcgcatgagc tggacgccgg
     6361 cgatcaccct cgggcaaggg cgaccctccg ggaggcgctg gggctctggc gtggccggcc
     6421 gctctcggac ttcgcctatg aggagttcgc gcagccggag atccgtcgcc tggagagcct
     6481 tcggctggac gctctcgagc aactggccgc cacagagctc gcgtccggcc gcgagcagga
     6541 ggtcctcccc ctgatcgagg cggtgatccg tgacgatccc cgcagggagc gctcccggga
     6601 gcttcagatg ctggccctgt accggaccgg tcggcaccct gaggcgctcc gagtgttcca
     6661 ggagttccgc ggacttctcg ccgacgagct tgggctggat ccttcgcccc ggctgcgacg
     6721 gctccaggag cagatcctcc tccacgaccc ttccctcgag cctcccgccc agccggggcc
     6781 caccccagcg gggccgcacc tgcggaaccc gtacaagggg ctccagccgt tcggcgagga
     6841 ggacgccaag gacttcttcg gccgggagga cctggtcaga cgcctgctcg acgccctgaa
     6901 gggggggatc cggctgctgg ccctggtggg tccctcggga tgcgggaagt ccagtgtcgt
     6961 gggggcgggc ctggtcccgg cgctccgtcg gggcgcgctc ccagggtccg accgatggat
     7021 gatcgtgcgg atgctgcccg gggcgcgtcc atccgaggag ctcgaggcgg cgctcctccg
     7081 agtcgcgggc gagccagggg cgcgcctcga acccctggac gggggggatg atggcctgct
     7141 ccgggcggca cgcgagttgg cgcctcgcgt cggcccgctc gtcctggtga tcgatcagtt
     7201 cgaagagctc ttctcgttgt cggacgagcc ctccagggcg gcattcctgc gcaacctggc
     7261 cacggccgtc ggcgacccgg gaggaccggt cgtgatcctc accctccggg gagacttcta
     7321 cgaccgtccc ctcctccacc cggagttcgc cgcccacttc acccgtggcg tggtgaacgt
     7381 ggttcccatg accgctacgg agattgaggc cgcggtggtg gggccggctc gccgggtggg
     7441 ggtcgaggtg gaccccgccc tgctggcgga gctgatcgcc gagacggtcg accgaccggc
     7501 ggggctgccg ctcctccagt acgccctcac cgagctcttc gtccgtcgct ccgagtccac
     7561 gctctcgctc gaggagtacc gcgccgtggg cggggtgcgt gcggccctgt cccgtcgcgc
     7621 cgaggagctg tacgggagcc tcgacgaaga ccggcggtgg gtggccatgc aggtcttcct
     7681 ccggctggtc cggctgggcg aggggaccag agacgctcgg cgccgggtgc cggtagggga
     7741 gctcaccgcg ctcgatctcg accccgtggc tctctccgag gtcctgaagg ccttcggccg
     7801 gcaccgcctc ctgtccttcg atcgcgatcc tgcgggcgga ggcgcgacgg tggaggtggc
     7861 ccacgaggcc ctcctgtcgg agtgggagcg cctggccgag tggatcgagg cccaccgtgg
     7921 agatctccgc cgccacggct cgctcgccgt ggccgtcgag gagtgggagt cttcgggccg
     7981 ccaccccgac tatctcctga ccgggaaccg gctggcggag ctcgatggat gggtccggtc
     8041 cacggcgctc gagttgaccg agtcggagcg tcggttcccg gccgccgggc tggagcggct
     8101 gcgggaggag caggctgggg aggcggagcg gctggagcag cagcggagac tggagcgccg
     8161 ggcccggacg aggctgtggg ccctggcggc cgctctcgtc ctgctggtcg gaggggccac
     8221 gtatggggtc ttggcattcc tgggcggggg ccctcccgac gcggtgctcg tgttcgaagg
     8281 gcccggcgat gggagcttca acgacatggc cgatgccgcc ttcgatcggg ccgccgctga
     8341 gctcggcctg gacgtggaca aagtgactgg gagccgcgcg agcctcgagt cggttctccg
     8401 acgggtgtcg gagcaggacg tgggcctggt cgtcttggct ttgcccgcgt tccctgacga
     8461 tgtcgagccg gtcgcccgcg accaccccga cactctgtac gtcctcaatt cggaaggacg
     8521 agggccggat gtcatcccga acgcgatcta catgaacttc gctaacgcgg agggctcctt
     8581 cttgatcggg gccgccgcgg ccctcaagag cgagacgggg acgatcgggt tcatcggggg
     8641 gctcgatttc cccttcatct ggcagttcca ggccgggtac gaggccgggg cccgagcggt
     8701 cgacccgggg atcgaggttc aggcggagta tctggcccgg ctacccgacg agagcggcgg
     8761 gttcgacagc cccacgctgg cggcacaggt cgccgatcgc cagttccgag ccggagccga
     8821 cgtcatctac cacgccgccg gtcgctcggg gctgggcctg ttcgataccg ccgaggctct
     8881 ctcggaggag ctgggcaggc acctgtgggc catcggggtc gacgccgatc aataccggtc
     8941 ggtcgtcgca ggggaggccc accccgcatg ggaacctgag gcctggcccc cccacatcct
     9001 cacctcgatg ttgaagcggt acgacaacgc actcttctcc atcctcgagg actactcccg
     9061 ggggatgctc acgccaggtg gccgggagct caacctggcc tctggggggg tggacatctc
     9121 atacaccggg gggttcatcg acgatatccg tccggagctg gaacggctcc ggcagcagat
     9181 catcgaggga gacatcgagg tccccaccat cccggcggag aaggctgggg ctgacttctg
     9241 accccccgcc aatcccccgc ctccacgtag agcctgcgta gagcgagcgt agtgccccgg
     9301 ttgaggaggg tcatccaccc tggctgccgg aaggaggtgg tgctacgtga agttggcacc
     9361 gacaagggag cacccggctc cggtggcgcc gggggaggtg gttctggccc cgcctcagcg
     9421 gcgcaggggg ctcagccgca ggctggtcct ggttacggtg gttctggcgc tcgtggccgg
     9481 tggcgtcgga gggtacgtcg cgtacctcgt ctcagaggac ggtctcactc ggggccgggc
     9541 gaccgacgcg gcccggtggg aggccatggg cgacgcccac ctccgagacc aggtggcgct
     9601 ggctcggggc cgggcggccg aggcggcccg gtggcaggcc ctggcggacg cgatccggga
     9661 ggggaccctg gcgggcccct gatcgcttcg cgggcgtccg ccgctcggcg ccgctgcgcc
     9721 caccatgagc atcccgctcg cggtgggcgt accggcgtgc tgagtgtcgc aggccagctg
     9781 ggcctgcgcg gcgttgtgga cagagaagtg ggatcggctc cgtgcagagg agctggagga
     9841 acgggtcgtg gacggcctcc tagccacgct tgagcaagac gacctcctcg aagaggcgct
     9901 cgccgagtgg agggggcttg ctgaggagag tctgcccagg cgggagacgg agcttgcccg
     9961 aatcgaggct cgcatccgga aggcgacgaa tcccctcgac cggcacttct tggcgttcga
    10021 gtaggggaag ctccgcgagg aggtgtgcac aacccgcatt gaggaactgt cgaaggagct
    10081 ggcgtcgctt gaggggcgcc ggtccaatct gatcgaggag atctcgggca gcaagcccga
    10141 actgccggac cctaaggagc tgaccgacct ccgcgaggat gtggagcgtt cgttgatggc
    10201 cgaggagttt cctcagcgga aagcggcgat gcaggcggtc gttgcggaga tcaaggtccg
    10261 gaaccgggcg cacatcccca gtcttccgcg taccaatatt tcgaccaccg tatggattgg
    10321 tacccccggc aggactcgaa cctgcgcccc tggctccgga ggccagtgct ctatccgctg
    10381 agctacgggg gcttgacccc catggtacag cgacgggaag gccgcagggg acccccctgg
    10441 tatcctcgcc cgcatggcgt ccgggcggat cctgatcgcg gacgacgacc cggtcatcct
    10501 gcggctgctc gaggtgaact tctccctcga gggcttcgac gtggagaccg ctgagcgggg
    10561 ggagcgggtc ctggaggagg ctcgccgggt ccggcccgac gtgatcgtcc tcgacgtgat
    10621 gatgcccggg atggacgggt gggaggtgtg cgaacggctg aaggaggacc cggggacggc
    10681 ggacatcccg atcatggtcc tctccgctcg gacccaggag caggatcgcg agcgcgggag
    10741 ggagctcggt gtgtcagcat acgtgaccaa gccgttcgac ccggaggagc tcatccgcgt
    10801 cgccaggagc ctcctgccgg aggagtgacg gcggccggcc catgagcgaa gccgaggtcc
    10861 tctcgttgat ccgtcgggcg ctcgaggatg cgcagggcga gctggggctc gccgagatcc
    10921 ccgagccgat cgaggtcgtc cgcccacggc agagggagca cggcgactac gctacgaacg
    10981 tcgcgctggc ggtgggggcg gccctgggga ggaatccgcg cgaggtcgcg gaggtcgtcg
    11041 tccgacacct ccccgagagc gacctggtca cgaaggccga ggtcgccggc ccgggcttca
    11101 tcaacttcac gctggctcac gtctggctcc atcgcgtcct gcacgaggtc gagcggcagg
    11161 gcgaggccta cggccgcgtg gacgaggggg gcggggagcg ggtgcaggtc gagttcgtct
    11221 cggccaaccc cacggggccc ctccacgtgg ggaccggtcg caacgccgcg gtgggcgacg
    11281 cgctcgcgtc gctgctggac gccgccggcc accgcgtcga gcgcgagtac tacgtgaacg
    11341 acgccggcac ccagatcacg ctgttcgcgg cctcgatcga ggcccgctac ctggagcggt
    11401 tcgggcacga ggcgaaggtg cccgagggcg gctacgaggg cgcgtacgtt cgcgagctgg
    11461 cggagcgcat cgccgaggac gtgggagagg agctgctgga cctgcctcaa gaggagcggc
    11521 tggcccggtt ccgggaggag gggatccgcc ggagcctcga cgggatccgg gccacgctgg
    11581 agcgcttcgg gatccggttc gacacctggt tcccggagtc ccagctcgtg gagagcggtc
    11641 ggatccacca ggcgatggag ctgctcaggg agcggggctt cgcctacgag gacgagggcg
    11701 cactgtggtt cgaggcctcc cggtacggcg acgagaagga ccgggtcttg atccgctcca
    11761 ccggggagca cacgtacttc gcggcggacg tggcctacct gcgggagaag ttcgcgcgag
    11821 gcttcgaccg cctcgtctac gtgtggggcg ctgaccacca cggcgcggtg aagcgcctgc
    11881 gcggcgcggc ccaggccctg gggtacgatc cggaggccgc ggagttcatc ctgctgcagc
    11941 tcgtggcgct gtaccggtcg ggagagcccg tgaagatgag caagcgcacg ggcgagatca
    12001 tcacactgga cgagctgctg gacgacgtgg ggccggacgc ggctcgctac acgctgctga
    12061 cgcgctcgcc cgacaccgcg ctggacttcg acatcgacct ggtggcgagc caatcgctgg
    12121 acaatcccgt ctactacgtg cagtacgccc acgcccggat cgccagcatc ctgcggttcg
    12181 ccgaggagcg gggcgtcggg atgcgcccgc tggacgaggt ggacctggac ctcctgcagg
    12241 acgagacgga gctcgccctc ctccgcaaga tcgcggagct ccccgagcag gtggccctgg
    12301 cggccaacct ccgggcgccg catcgcctga cccgctacgt ggagacggtg gccgccgggt
    12361 tccaccgctt ctacacggcg tgccgggtcg tgaccgacga cgaggagctg acccaggcgc
    12421 gcctgcacct cgcgggggcc gcgcgccggg tgatcgcgaa cgtgctcgcc atcatcgggg
    12481 tctcgg
//
LOCUS       DASUTW010000295         8863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_98514, whole genome shotgun sequence.
ACCESSION   DASUTW010000295 DASUTW010000000
VERSION     DASUTW010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8863
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98514"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1231)
                     /gene="sat"
                     /locus_tag="VF058_10105"
     CDS             complement(74..1231)
                     /gene="sat"
                     /locus_tag="VF058_10105"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEX6208692.1"
                     /translation="MTDTIDPHGGELVDLVLPEAEAAAAAEDAQHMPKIELGPREIAD
                     LEMLAVGAMSPLTGFMTEKDYTSVLHEMRLANGLVWTLPITLSVDEEGMKQVGRSDRV
                     ALTIGGEIHAILDVAETFRRDREVEAESAFGTTDDAHPGVAALRAAGDWVLGGSVSAL
                     KVPAHGTFVEHRRTPSETRRAFAERGWKRVVGFQTRNPVHRAHEYIQKCALEITDGLL
                     LHPLMGETKADDIPADVRMRCYLVLLDGYYPKDRVLLSIFPAAMRYAGPKEAIFHAIV
                     RKNYGCTHFIVGRDHAGVGDFYGTYDAQRIFDEFEGSDLGITPLFFEHAFWCERCEGM
                     ASTKICPHGEEDRISLSGTKVREMLRAGERPPMEFSRPEVADVLIEAMREN"
     gene            1285..2019
                     /locus_tag="VF058_10110"
     CDS             1285..2019
                     /locus_tag="VF058_10110"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX6208693.1"
                     /translation="MPPLDLPALNARFEDADPAQIVAWALDASERPAVASSFQVESTV
                     VIHLATRIRPEVPVLFLETGFHFRETLELKDGLTRMLGLNVVELRGETTPQEQAEAHG
                     PRLYERDPSLCCELNKVRPLDRALAAYDLWMTGLRRSSASTRARTRVVERHRTGEGHE
                     LLKVNPVAGWTHARARAYLEEHGLPHHPLYDLGYASIGCAPCTRALFPGEDERAGRWA
                     GVEKVECGIHLPPPATATAPPPSSHA"
     gene            complement(1988..3103)
                     /locus_tag="VF058_10115"
     CDS             complement(1988..3103)
                     /locus_tag="VF058_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX6208694.1"
                     /translation="MAERPRSDHWSDPRPPAWSRRLILQGLGLLALAWLTWTFLSRLS
                     QLLLWILIALFLSFALEPAVNWLSKRGMRRGVATGLIFLAAALVGILSIAVMVPFVVQ
                     GVLAVVRNLPSWLDQASRLTEDWFGVTVSTEDLRREIFSAASTLQSFAGDIVGNVIGV
                     GGAALGAVFQVFTIGFFLFYFVAEGPRVRRGVCSLLPRDKQDDVLFVWGTAIDKTGGY
                     FYSRLLLALINGITFFAVLFLLGIPGAPALALFSAVVATFIPIVGTYVGAAVPLLVAL
                     ASVGVARTLVVLGWVVLYQQIENLFLAPKISASTMELHPAVAFSAALAGGLLGGVVWA
                     FLALPIAATAQAAAGAYIERYEVIEDDLTRETTEEEP"
     gene            complement(3096..3824)
                     /locus_tag="VF058_10120"
     CDS             complement(3096..3824)
                     /locus_tag="VF058_10120"
                     /inference="COORDINATES: protein motif:HMM:NF022655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HEX6208695.1"
                     /translation="MVRPVRRDAPGPRGPGRAILITVLVLLLLAVAVDLGLRFLAERW
                     LEDRVRQTLALERRPDLDIGGFPFVTQFVRGEVERVDVTLRDLRVQGLHLDEVTIGLR
                     RVAFDRVDVLAGRSGSVRVRRGAATVEIDEPTLNGVLRDRGLPVSVELLGPRVRATSR
                     IEVEGRRATASAEGTLDLEGTTLVFDPGEVSVEGAFGVPPAALAFRVDLPLLFPGLTY
                     EDVRVREAVLALRADLDDTVLDLG"
     gene            complement(3825..4220)
                     /locus_tag="VF058_10125"
     CDS             complement(3825..4220)
                     /locus_tag="VF058_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007981659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX6208696.1"
                     /translation="MSLEGKALELFEGPNFAHLATINPDGSPQVSPVWVHARDGLIEL
                     NTSRGRVKDRNMTKDPRVALSITDANDPYIRASVQGEVVEQTEEGAVEHIDFLSEKYD
                     GVTPYPRLTPGMVRVIYRIRPGRVHVTGG"
     gene            complement(4253..4852)
                     /locus_tag="VF058_10130"
     CDS             complement(4253..4852)
                     /locus_tag="VF058_10130"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX6208697.1"
                     /translation="MRDVERALRGGSMPEGAVERFVERAWNEVDGVVAYAGVDSPFGR
                     LIVAATRRGIVCLAYPGEADAVQELAERLTPRLVEAPERLDEARRELDDYFEGRRDRF
                     DLAVDLSLVRGEFTRKVLRATSRIPFGRTLTYTEVATKAGSPRGSRAAGNALGSNPIP
                     IVVPCHRVIHASGGLGGYTGGLDRKEFLLRLEGVLGETG"
     gene            complement(4849..5349)
                     /locus_tag="VF058_10135"
     CDS             complement(4849..5349)
                     /locus_tag="VF058_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6208698.1"
                     /translation="MTSRNRAPLRSSGVGLPPFMDVLEEHGDMVYRYLRVAVGPEDAE
                     DCLQETLLSALRAYPDLADARNLRGWLLTIAKRKALDEHRARGRRPSPSGRLPEAAID
                     PPTEAEDGLWRAVRELPPKQREAVVYRFVCDLAHREIAELMSSTEAAARRNVHEGLRA
                     LREVWR"
     gene            complement(5346..6107)
                     /locus_tag="VF058_10140"
     CDS             complement(5346..6107)
                     /locus_tag="VF058_10140"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6208699.1"
                     /translation="MIARLLSRRYRSAAPFAATTADGVPLVGARLGRGPAALVLCHGF
                     MGWHRKARIVSFAERLAERFLVFVFDLRGHGGSAGVCTYGDREYLDVDAIVGLARREA
                     GLPVATLGASMGGIAVIRQAALGEPPDAVMAVSTPARWEGHATAAVRRVTRMTATPSG
                     RRMMRALGFRLTDTWGSPEDPERLVEKVAPTPLVIVHGRDDHYFDEENAWRLYARAGE
                     PKRLMLASRFGHAEDGFTPAFADLAARRLLEILPT"
     gene            6159..6830
                     /locus_tag="VF058_10145"
     CDS             6159..6830
                     /locus_tag="VF058_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6208700.1"
                     /translation="MRRLLILTDRRPGDVLPAAHALGTDVKTDSLDAGGLARVPDLGP
                     DLVVADSVPDPERCFGLLRELRARGGPPALVVLGRDQLAILPWQEVADDFVHPETGED
                     ELRLRVELLRSRVGETGEGVLRLGPVAINTLTYRVTAGGRALDLTFKEFELLRFLAQH
                     EGRVFTRAQLLREVWGYDFYGGTRTVDVHVRRLRAKLGPEHEHLIETVRSVGYRASSG
                     EPPLD"
     gene            7083..8477
                     /gene="glnA"
                     /locus_tag="VF058_10150"
     CDS             7083..8477
                     /gene="glnA"
                     /locus_tag="VF058_10150"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX6208701.1"
                     /translation="MAKDAGAEVVDLRFCDLPGLMQHFSVPVSELTEESFEDGFGFDG
                     SSIRGFQEIQESDMLLLPDPGAAYVDPFRPRPTLVLHCFVKDPVTGEMYTRDPRGIAR
                     KAELYLQQTGIAETSYWGPECEFYIFDGIRFDQNQHEGYYHIDAMEGVWNSGRDEEGG
                     NKGYKPRYKEGYFPVPPMDHYQDLRTEMMLNLERVGIQVEVHHHEVGTAGQAEIDIRY
                     DTLLRTADKVMTYKYVVKNTALAAGKTVTFMPKPIFEDNGSGMHTHQSLWSADENLFW
                     DEMGYAGLSDSARWYIGGLLQHAPALLAFAAPTTNSYRRLVPGYEAPINLVYSQRNRS
                     ACIRIPVYYKTPVAKRIEFRSPDPTANPYLAFSAMLMAGIDGIKNKTEPPDPIDKDLY
                     DLPPEEAAEVRQVPGSLDEVIAALESDHAFLLEGGVFTQDVVDTWIDYKRRNEIDELR
                     LRPHPYEFYRYFDA"
     gene            complement(8462..>8863)
                     /locus_tag="VF058_10155"
     CDS             complement(8462..>8863)
                     /locus_tag="VF058_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208702.1"
                     /translation="VPAGPMIEPSLVLVRDDLQAEAAAANAPAESAADLIRSWDDARA
                     LLVVYGGTVPDAGTSVRVEDVALEPEARHLIVTATLESEDPALQVVSIAWAAVSLDAD
                     LVARADTCALVLDGREVPDAACSVTIPYASK"
BASE COUNT         1383 a         3208 c         3082 g         1190 t
ORIGIN      
        1 tgaggatcgg gagggctaac gagccagcag gcaggccgtc ggggttcctg aggcgcccgc
       61 gacggccatc ccctcagttc tcgcgcatcg cctcgatcag gacgtcggcg acctcggggc
      121 ggctgaactc catcgggggg cgctcgcccg cccggagcat ctcgcgcacc ttcgtgccgg
      181 agagcgagat cctgtcctcc tcgccgtggg ggcagatctt ggtggaggcc atcccctcgc
      241 accgctcgca ccagaaggcg tgctcgaaga agagcggcgt gatgcccagg tcggacccct
      301 cgaactcgtc gaagatccgc tgggcgtcgt aggtcccgta gaagtccccc acgccggcgt
      361 ggtctcgccc cacgatgaag tgcgtgcagc cgtagttctt ccggacgatc gcgtggaaga
      421 tggcctcctt cgggcccgcg tagcgcatcg ccgccgggaa gatcgacagg aggacgcggt
      481 ccttcggata gtagccgtcg agcaggacca ggtagcagcg catccgcacg tcggcgggga
      541 tgtcgtcggc cttcgtctcc cccatcagcg ggtgcagcaa gagcccgtcg gtgatctcga
      601 gagcgcactt ctggatgtac tcgtgggccc ggtgcaccgg gttgcgggtc tggaacccga
      661 ccacgcgctt ccagccgcgc tcggcgaagg ctcgccgcgt ctcggacggc gtgcgccggt
      721 gctcgacgaa cgtcccgtgc gcggggacct tgagcgccga cacgctgccg ccgagcaccc
      781 agtcgcccgc cgcgcgcagg gcggcgacgc cggggtgggc gtcatcggtg gtcccgaacg
      841 cggattcggc ctcgacctcc cggtcccgcc ggaacgtctc ggccacgtcc aggatcgcgt
      901 ggatctctcc gccgatcgtc agcgccacgc ggtcgctccg gcccacctgc ttcatcccct
      961 cctcgtccac cgagagggtg atgggcaggg tccagaccag gccgttcgcc aagcgcatct
     1021 cgtgcaggac ggaggtgtag tccttctcgg tcatgaagcc ggtgagcgga gacatcgcgc
     1081 ccacggccag catctccagg tcggcgatct cgcggggccc cagctcgatc ttgggcatgt
     1141 gctgggcgtc ttcggcggcg gccgcggcct cggcctccgg gaggacgagg tccaccagct
     1201 cgccgccgtg ggggtcgatc gtgtcggtca tgggcttcct cgggtcgggg tccgcccgag
     1261 gatagcagtc ctatacttgg ccccgtgcca cccctcgacc tcccagctct caacgcgcgc
     1321 ttcgaggacg cggaccccgc ccagatcgtg gcgtgggcgc tcgacgcctc ggagcggccg
     1381 gccgtggcgt catcgttcca ggtcgagagc acagtcgtga tccatctcgc cacgcgcatc
     1441 aggcccgagg tgcccgtgct cttcctcgag accgggttcc acttccggga gacgctcgag
     1501 ctgaaggacg ggctcacccg catgctcggg ctgaacgtcg tggagctccg gggggagacc
     1561 accccgcagg agcaggcaga ggcccatggc ccccggctgt acgagcgcga cccctccctc
     1621 tgctgcgagt tgaacaaggt gcggccgctc gaccgggccc tcgccgcata cgacctctgg
     1681 atgacgggcc tccgccggag ctcggcctcc acgcgggccc gaacccgcgt ggtggagcgc
     1741 caccggaccg gggaaggaca cgagctgctc aaggtgaacc cggtggcggg atggacccac
     1801 gcccgggccc gggcctatct ggaggaacac gggctccccc accacccgct ctacgacctg
     1861 gggtacgcct cgatcggctg cgcgccctgc acccgggcgc tcttccccgg cgaggacgag
     1921 cgggcggggc ggtgggccgg ggtggagaag gtcgagtgcg ggatccacct cccgcccccg
     1981 gcgaccgcta cggctcctcc tccgtcgtct cacgcgtgag gtcgtcctcg atcacctcgt
     2041 accgctcgat gtaggcgccg gccgctgcct gcgcggtggc ggcgatcggg agggcgagga
     2101 aagcccagac cacgccgccc agcagtcctc cggcgagcgc cgccgagaag gccacggccg
     2161 gatggagctc catcgtgcta gcgctgatct tgggggccag gaagaggttc tcgatctgct
     2221 ggtagagcac gacccacccc aggacgacca gcgtccgggc cacgcccacg ctggccagcg
     2281 ccacgagcag ggggacggcg gcccccacgt aggtccccac gatcgggatg aaggtggcga
     2341 cgaccgcgct gaagagggcc agcgccggag cgcccgggat ccccagaagg aacaggaccg
     2401 cgaagaacgt gatgccgttg atcagcgcga ggaggaggcg cgagtagaag tagccgcccg
     2461 tcttgtcgat cgccgtgccc cacacgaaga ggacgtcgtc ctgcttgtcc cgcgggagca
     2521 gcgagcacac gccccgccgg acccgaggcc cctccgccac gaagtagaag aggaagaacc
     2581 cgatcgtgaa gacctggaac actgccccga gcgccgcgcc gcccaccccg atcacgttgc
     2641 ccacgatgtc gccggcgaag gactggagcg tcgaggccgc ggagaagatc tccctgcgga
     2701 ggtcctccgt cgagaccgtg accccgaacc agtcctcggt gagccggctg gcctgatcga
     2761 gccacgacgg gaggttccgg accacggcca gcacgccctg caccacgaag gggaccatga
     2821 cggcgatcga gaggatcccc acgagcgcgg cggccaggaa gatcaggccc gtggccacgc
     2881 cacgccgcat gccccgcttg gacagccagt tgacggcggg ctcaagcgcg aacgagagga
     2941 agagggcgat caagatccac agcagcagct gactcagccg ggaaaggaac gtccaggtga
     3001 gccaggccag cgccagcaag cccaggccct gcaggatgag ccgcctgctc cacgcggggg
     3061 gtcgcggatc gctccagtga tcggaccgcg gtcgctcagc caaggtcgag gacagtatcg
     3121 tccaggtcgg cgcgcagagc gaggaccgcc tcgcgcaccc ggacgtcctc gtaggtcagg
     3181 cccgggaaga ggagcggcag gtccacgcgg aaggcgaggg ccgcgggcgg caccccgaac
     3241 gcgccctcca cgctgacctc gccgggatcg aagaccagcg tggtcccctc gaggtcgagg
     3301 gtcccctcgg cggaggccgt cgccctccgt ccctcgacct cgatccgcga cgtggcgcgg
     3361 acgcggggac ccagcagctc cacgctgacg ggcagacctc ggtcgcggag caccccgttc
     3421 agcgtgggct cgtcgatctc caccgtggcc gccccccggc ggacgcgcac cgacccgctc
     3481 cgtccggcca gcacgtcgac ccggtcgaag gccaccctcc ggagcccgat cgtcacctcg
     3541 tccaggtgga gcccctggac cctcaggtcc cggagggtga cgtccacgcg ctcgacctca
     3601 ccccggacga actgggtaac gaaggggaag cccccgatgt ccaggtccgg ccggcgctcc
     3661 agggccaggg tctgccgcac ccgatcctcg agccagcgct cggccaggaa gcgcaggccc
     3721 aggtcgacgg ccacggccag caggagcagg acgaggaccg tgatcaggat ggcccggccg
     3781 ggcccccggg ggccgggcgc gtccctgcgg accgggcgga ccactcagcc gccggtgacg
     3841 tgcacccggc ccgggcggat gcggtagatc acccgcacca tgccgggcgt gagcctcggg
     3901 tagggcgtca cgccgtcgta cttctccgag aggaagtcga tgtgctccac cgcgccctcc
     3961 tcggtctgct ccacgacctc accctgcacc gaggcccgga tgtacggatc gttcgcatcg
     4021 gtgatcgaga gcgcgaccct cggatccttc gtcatgttcc ggtccttcac ccgcccccga
     4081 gaggtgttca actcgatcag tccgtcacgg gcgtggaccc agaccggtga gacctgcggc
     4141 gacccgtcgg ggttgatcgt cgccaggtgc gcgaagttgg gtccctcgaa cagttcgagc
     4201 gccttgccct ccaggctcat tcgttcctcc tcagggtcgg tctcctcgaa gattacccgg
     4261 tctcgccgag cacaccctcg agcctgagga ggaactcctt gcgatcgagc ccgcccgtgt
     4321 agccgcccag cccgccggag gcgtggatca cccgatggca ggggacgacg atcgggatcg
     4381 ggttcgatcc cagcgcgttg cccgccgccc tcgagccgcg ggggctgccg gccttcgtcg
     4441 ccacctcggt gtaggtgagc gtcctcccga acgggatccg cgaggtggcc cggagcacct
     4501 tgcgggtgaa ctcgccgcgg acgagcgaga ggtccaccgc caggtcgaag cggtcgcgcc
     4561 tgccctcgaa gtagtcgtcg agctcgcgcc gcgcctcgtc gaggcgctcc ggcgcctcga
     4621 cgaggcgcgg cgtcagccgc tcggccagct cctggaccgc gtccgcctcg cccggatagg
     4681 cgaggcagac gatcccccgc cgcgtggccg ctacgatcag ccgaccgaac ggcgagtcca
     4741 cgccggcgta cgccacgacg ccgtccacct cgttccaggc gcgctcgacg aagcgctcga
     4801 ccgccccctc gggcatcgac ccgccccgca gcgcccgctc cacgtctctc atctccaaac
     4861 ctcccgaagg gcccgcagcc cctcgtgcac gttccgcctg gccgcggcct cggtcgagct
     4921 catcagctcc gcgatctcgc gatgcgccag gtcgcacacg aaccggtaca cgaccgcctc
     4981 ccgctgcttc ggagggagct cccgcaccgc ccgccagagc ccgtcctcgg cctcggtggg
     5041 cgggtcgatc gccgcctcgg gcagccgccc cgagggcgag ggccggcgcc cccgggcccg
     5101 gtgctcgtcg agcgccttcc gcttcgcgat ggtgagcagc cacccgcgga ggttcctggc
     5161 gtcggccagg tccgggtagg cgcgcagggc cgacaggagc gtctcctgga gacagtcctc
     5221 cgcgtcctct ggccccaccg cgacccgcag gtagcggtac accatgtcgc cgtgctcctc
     5281 gagcacgtcc atgaacggcg gcagtcccac gcccgaagaa cggagcgggg cgcggtttcg
     5341 tgaggtcacg tcgggaggat ctcgaggagg cggcgagccg cgaggtcggc gaaggcgggg
     5401 gtgaagccgt cctcggcgtg gccgaagcgc gaggccagca tcagccgctt cggctccccc
     5461 gcccgcgcgt agagacgcca ggcgttctcc tcgtcgaagt agtggtcgtc gcggccgtgg
     5521 acgatgacga ggggggtagg ggccaccttc tccacgagcc gctcgggatc ctccggcgac
     5581 ccccaggtgt cggtcagacg gaaccccagg gcccgcatca tccggcggcc cgacggcgtg
     5641 gccgtcatcc gagtcacgcg ccggaccgcg gcggtcgcgt gcccctccca ccgcgcgggc
     5701 gtggacacgg ccatcaccgc gtcgggaggc tcgccgagcg cggcctgacg gatcaccgcg
     5761 atcccgccca ttgaggcacc cagggtggcc accgggaggc ccgcctcgcg gcgggccagc
     5821 cccacgatcg cgtccacgtc aaggtactcg cggtcgccgt acgtgcagac gccggcgctc
     5881 ccgccgtgcc cccggaggtc gaagacgaag acgaggaacc gctcggccag ccgctccgcg
     5941 aacgagacga tgcgcgcctt gcggtgccat cccatgaagc cgtggcagag gacgagcgcg
     6001 gccggacccc ggcccaggcg ggccccgacg agcgggacgc cgtcggccgt ggtcgccgcg
     6061 aacggggcgg ccgaccgata gcggcgggag aggagccgcg cgatcacgga ggcaagggta
     6121 cctcccccga tcgaggggcc gatgctacgc tcgtcccggt gcgacggctc ctcatcctca
     6181 cggatcgccg ccccggcgac gtgctccccg ccgcgcacgc cctggggacg gacgtcaaga
     6241 ccgactcgct ggacgccggc gggctggccc gcgtccccga tctcggcccc gacctggtgg
     6301 tggccgactc cgtccccgac cccgagcgct gcttcggact cctccgcgag ctgcgggcgc
     6361 gcggcggccc accggccctc gtggtcctgg ggcgggatca gctcgcgatc ctgccctggc
     6421 aggaggtggc cgacgacttc gttcatccgg agaccgggga ggacgaacta cggctccgcg
     6481 tggagctcct gcgatcccgc gtgggggaga ccggcgaggg cgtgctccgg ctggggccgg
     6541 tggcgatcaa cacgctgacc taccgcgtca cggccggggg ccgcgccctc gacctcacgt
     6601 tcaaggagtt cgagctgctg cgcttcctcg cccagcacga gggccgcgtc ttcaccaggg
     6661 cccagctgct ccgcgaggta tggggctacg acttctacgg aggcacccgg acggtcgacg
     6721 tccacgtccg gcggctgcga gccaagctcg gcccggagca cgagcacctg atcgagacgg
     6781 tccgcagcgt gggctaccgc gcctcgtccg gcgagccccc actagactaa gcgcgtgatc
     6841 cctggcgtcc cgatccctgt gatcgagaac gccagcgtcg cggccagcgc ggtcgcgagg
     6901 acccgggccc ttgctctgga tcgcatgctg ccgaggggac cagcgaatcc tcgatacctc
     6961 aaccccgatc cgttacagag agttaacggg caggagacgc tccggtgacg accgcccggt
     7021 aggcttccgg acatccgagc gaaggagggt tgcgcgtgag cacgccgaag gacgtcctgg
     7081 cgatggcgaa ggacgcggga gcggaggtgg tcgacctccg gttctgcgat ctccccgggc
     7141 tgatgcagca cttctccgtc cccgtgtccg agctgaccga ggagagcttc gaggacgggt
     7201 tcgggttcga cgggtcctcg atccgcggct tccaggagat ccaggagtcg gacatgctgc
     7261 tcctgccgga ccccggggcc gcctacgtcg accccttccg tccccgcccc accctggtgc
     7321 tgcactgctt cgtgaaggac ccggtcaccg gcgagatgta cacgcgcgac ccacgcggca
     7381 tcgcccggaa ggccgagctc tacctccagc agacggggat cgcggagacc tcctactggg
     7441 gcccggagtg tgagttctac atcttcgacg ggatccgctt cgaccagaac cagcacgagg
     7501 gctactacca catcgacgcg atggagggcg tgtggaactc gggccgggac gaggaggggg
     7561 ggaacaaggg gtacaagcct cgctataagg agggctactt ccccgtcccg ccgatggacc
     7621 actaccagga cctccgcacc gagatgatgc tgaacctgga gagggtcggg atccaggtcg
     7681 aggtccacca ccacgaggtg gggacggcag ggcaggccga gatcgacatc cggtacgaca
     7741 ccctgctgcg gaccgccgac aaggtgatga cctacaagta cgtcgtcaag aacacggcgc
     7801 tcgcggccgg caagacggtg acgttcatgc cgaagccgat cttcgaggac aacgggtccg
     7861 ggatgcacac gcaccagagc ctgtggtccg ccgacgagaa cctgttctgg gacgagatgg
     7921 ggtacgcggg gctgtcggac tcggcgcgct ggtacatcgg gggccttctg cagcacgcgc
     7981 cggcgctcct ggcgttcgcc gcgccgacca cgaactccta ccgcaggctc gtccccgggt
     8041 atgaggcacc gatcaacctc gtctactccc agcggaaccg ctccgcctgc atccggatcc
     8101 ccgtctacta caagacgccg gtggccaagc ggatcgagtt ccgctcgccc gaccccacgg
     8161 ccaacccgta cctcgcgttc agcgcgatgc tgatggcagg gatcgacggg atcaagaaca
     8221 agaccgagcc gccggacccg atcgacaagg acctctacga cctcccgccc gaggaggcgg
     8281 ccgaggtccg tcaggtcccc gggtcgttgg acgaggtgat cgccgcgctc gagtcggatc
     8341 acgcgttcct gctggagggc ggggtcttca cccaggacgt cgttgacacc tggatcgact
     8401 acaagcgcag gaacgagatc gacgagctgc ggctgcggcc gcacccctac gagttctacc
     8461 gctacttcga cgcgtagggg atcgtcacgg agcacgccgc gtcggggacc tcgcggccgt
     8521 caaggacgag cgcgcaggta tcggcgcggg ccacgaggtc ggcgtcgagc gagaccgcgg
     8581 cccaggcgat cgagacgacc tggagcgcgg gatcctccga ctcgagcgtc gcggtgacga
     8641 tcaggtggcg tgcctccggc tccagcgcga cgtcctcgac gcggaccgag gtcccggcgt
     8701 cgggcacggt ccccccgtag acgacgagca gggcccgcgc gtcgtcccag gagcggatca
     8761 gatcggcggc gctctcagcg ggggcgttgg cggccgcggc ctccgcctgg aggtcgtccc
     8821 ggaccaggac gagcgagggc tcgatcatgg ggccggcggg cac
//
LOCUS       DASUTW010000296         6128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1782320, whole genome shotgun sequence.
ACCESSION   DASUTW010000296 DASUTW010000000
VERSION     DASUTW010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1782320"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VF058_10160"
     CDS             complement(<1..726)
                     /locus_tag="VF058_10160"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6208703.1"
                     /translation="MRIVVTGAGGGVGRALREAAPAHHEIEWLAHDDLSVEDRHAVMQ
                     RVAGADPDVILHLAAETKVDDCERDPDAAYRANALGTANVALAARETGALLVALSTDY
                     VFDGTKGEPYHEFDRPNPISVYGTSKLAGEEEARALAPDHLIVRTAWVFGGGDDFVTG
                     AVRRLASGEEVAAIVDLHGTPTYVRHLAERLFPLAVSGLRGVVHLGGPERTTFFDLLA
                     RARGLGGLPGTPVEQKDADLGRPA"
     gene            complement(723..1670)
                     /gene="rfbB"
                     /locus_tag="VF058_10165"
     CDS             complement(723..1670)
                     /gene="rfbB"
                     /locus_tag="VF058_10165"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018959998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX6208704.1"
                     /translation="MNILVTGGAGFIGSHFVDLAMADPSRRVTVLDALTYAGTRENLE
                     GHREDERMRLIVGDVADPEAVREALEGADVAVHFAAESFVDRSIEAADPFVRTNVQGT
                     LTLLTACREAGVPMVQVSTDEVYGSIEDGAFTEDSPLAPNNPYAATKAAADLLCRSFR
                     ATFGVDVRILRGTNAYGPRQHREKAIPVFIAAALGGRPLPVYGDGSNRREWLHVTDFA
                     RAVRTVVEQGEPGGVYNAGGGRELANVDLAREICRLAGAAEELVSFVEDRPGHDLRYS
                     MLWERIAALGWKPEVPFDEGLAGTVEWFRAHPERLEGSP"
     gene            complement(1667..2131)
                     /locus_tag="VF058_10170"
     CDS             complement(1667..2131)
                     /locus_tag="VF058_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012036817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEX6208705.1"
                     /translation="MADRIEGVWVKDLRFIPDERGRLIEILRSDDEGFRKFGQVYMTT
                     TYPGVVKAWHLHKVQDDNFCCLKGMVKLVMYDARDASPTKGTINELFIGDHDPKLVRV
                     PPGVYHGWKGVSEEEAIVLNVPTEPYNHEAPDEYRAAWNTPDIPYSWDIVFK"
     gene            complement(2156..3217)
                     /locus_tag="VF058_10175"
     CDS             complement(2156..3217)
                     /locus_tag="VF058_10175"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HEX6208706.1"
                     /translation="MKALILAGGEGTRLRPITHTSSKQLIPVANTPILFYGLQAVADA
                     GIREVGIVVGDTAEEIEQACGDGSRWGVEITYIPQEAPLGLAHAVLISRDFLGDDDFV
                     MYLGDNILLEGVSRFVRQFEEHAPDAQIFLAKVPEPERFGVAVLEGDRIVRLVEKPKD
                     RISDLALVGVYLFDASVHEAVASVEPSWRGELEITDSIQYLIDHGKNVRAEMVEGWWK
                     DTGKLEDLLEANRMMLSRLEGGVEGEVDDASRLVGPVVVAPGAKVTRSVIRGPSIVGE
                     GTTIEDSLIGPYASIYTGCEITGSEVEDSIVMERCRIHGVPGIAESLLGKDVEVSRGR
                     RGRAAHRLMLGDQSQVEVL"
     gene            complement(3214..4527)
                     /locus_tag="VF058_10180"
     CDS             complement(3214..4527)
                     /locus_tag="VF058_10180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX6208707.1"
                     /translation="MSETKPTGKRRLLTERRLRNVGLVLVGVVTLVAFAVSSAALGFL
                     WYADSRVNRIPEDQTPDIVDVPEDPLDPINVLILGSDTREGLEEEEEQFGSPEMVEGE
                     RSDTIILGHFDPQEERAVLVHFPRDLRVEIPGHGEDKINAAFTLGGPNLAIKTVSRLT
                     GIPIHHYIEVNFNSFRTIVAALGGVEVCIDRPMIDPMAELELPEAGCYQMDGDTALAF
                     VRARNVEGDLIPDFARISRQQQFIRAVTNKLFSLGSVTRIPELVRAVAGSVRTDHALT
                     VTDLLDYGRELQGLAAEDPTGASTVDLRIVPGLAGPSFVEMTPEAEALFRRLREDRPL
                     GDLGTAPPGEVPPSPAQIAVKVVGAGRAAVAARAEDLLRDAGFIVLPGDRRPPREASE
                     IVHAAGAEAMAQVVAGYFPDLTLRQGGRALLGEADVAVVLGEDAP"
     gene            4555..>6128
                     /locus_tag="VF058_10185"
     CDS             4555..>6128
                     /locus_tag="VF058_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6208708.1"
                     /translation="MSDIVWEPTEEIIERANVTRFMRAHGIETYEELVRRSRDDVEWF
                     WDAVVKDLDIPFLEPYERVLDTSDGIPWARWFVGGKTNLAHATCDRWAEETPDAVAVL
                     WEGEDGPVRSVTYRELREMADRLANALASLGVGERDAVGIFMPMAPETVAAVLACAKL
                     GAVYLPIFSGYAADAVATRLRDADAKVLLTADGFPRRGSQVAMKEIADEAADASPTVE
                     KVVVWSRLGRSDLPWRADRDLRWEELTEKASPSFETLSLDSEHPLFIAYTSGTTGRPK
                     GSVHVHGGFTVKIAEEVAYQTDVQRGDVLFWVSDLGWIMGPWEITGAGALGATVFLYE
                     GAPNHPQPDRIWDMVERHRITHLGISPTLIRALIPAGDEWVERHDLSSLRILGSTGEP
                     WNPDPYLWFLEKVGGGRCPIINLSGGTEIGACFLSPLPINALKPATLRGPALGMAMDV
                     WGADGKPVAPGEVGELVCTKPWPAMTRGVWGDPQRYLETYWSRWPDVWVHGDWASVDE
                     DGFWFLHGRSDDTMNIA"
BASE COUNT          879 a         2287 c         2048 g          914 t
ORIGIN      
        1 ggccgggcgg ccgaggtccg cgtccttctg ctccaccggg gtccccggca ggccgcccag
       61 gccgcgcgcc cgcgccagta ggtcgaagaa cgtcgtccgc tccggccctc cgaggtggac
      121 gacgccgcgc agccccgaga cggccagcgg gaacagccgc tcggccaggt gccgcacgta
      181 ggtcggcgtc ccgtggaggt cgacgatcgc cgccacctcc tcgccgctcg ccagccgccg
      241 gacggccccc gtgacgaagt cgtcgccgcc cccgaagacc caggccgtcc gcacgatcag
      301 gtggtcgggg gcgagagccc tcgcctcctc ctcccccgct agcttcgagg tcccgtagac
      361 cgagatcggg ttcggccggt cgaactcgtg gtacggctcg cccttcgtcc cgtcgaacac
      421 gtagtccgtg gagagcgcca cgagcagcgc ccccgtctcc ctcgcggcca gcgccacgtt
      481 cgccgtcccc agcgcgttcg cgcggtaggc ggcatcggga tcccgctcgc agtcgtccac
      541 cttcgtctcg gccgcgaggt ggaggatcac gtcggggtcg gcgcccgcca cccgttgcat
      601 gaccgcgtgg cgatcctcga ccgagaggtc gtcgtgagcc agccactcga tctcgtggtg
      661 ggccggggcc gcctcgcgca gggccctgcc cacgcccccg cccgccccgg tgacgacgat
      721 cctcacggcg agccctccag ccgctccgga tgcgcgcgga accactcgac ggtccccgcc
      781 aggccctcgt cgaacggcac ctcgggcttc cagcccagcg ccgcgatccg ctcccacagc
      841 atcgagtacc ggaggtcgtg cccggggcgg tcctccacga acgagacgag ctcctcggcg
      901 gcacccgcga gccggcagat ctccctggcc aggtcgacgt tggcgagctc ccgcccgccg
      961 ccggcgttgt agacgccgcc gggctcgccc tgctccacca cggtccggac cgcccgggcg
     1021 aagtcggtca cgtgcagcca ctcccgacgg ttcgacccgt ccccgtacac ggggagcggc
     1081 cgcccgccga gcgccgccgc gatgaacacc gggatcgcct tctcgcgatg ctgcctgggc
     1141 ccgtaggcgt tcgttccccg caggatccgc acgtccaccc cgaacgtcgc ccggaacgac
     1201 cggcacagga ggtcggccgc ggccttcgtc gccgcgtagg ggttgttggg ggcgagaggc
     1261 gagtcctcgg tgaaggcgcc gtcctcgatc gagccgtaga cctcatcggt ggagacctgg
     1321 accatgggaa cgccggcctc ccggcacgcc gtcagcagcg tgagcgtccc ctggacgttc
     1381 gtccgcacga acggatccgc ggcctcgatg ctccgatcca cgaacgactc ggccgcgaag
     1441 tggaccgcga cgtccgcgcc ctccagcgcc tcccgaacgg cctcggggtc ggccacgtcg
     1501 cccacgatca gccgcatgcg ctcgtcctcc cgatgcccct cgaggttctc cctcgttccc
     1561 gcgtaggtca gcgcgtcgag gaccgtcacc cgacggctcg ggtcggccat ggcgagatcc
     1621 acgaagtggg agccgatgaa cccggctcct cccgtgacca ggatgttcac ttgaagacga
     1681 tgtcccagct gtaggggatg tccggcgtgt tccacgccgc ccggtactcg tcgggcgcct
     1741 cgtgattgta cggctccgtc ggaacgttga ggacgatcgc ctcctcctcc gacacgcctt
     1801 tccagccgtg gtacacgccg ggcgggaccc tcaccagctt ggggtcgtga tcgccgatga
     1861 acagctcgtt gatcgtgccc ttcgtcgggg aggcgtcgcg ggcgtcgtac atcacgagct
     1921 tcaccatccc cttgaggcag cagaagttgt cgtcctggac cttgtggagg tgccaggcct
     1981 tcaccacccc cgggtaggtc gtggtcatgt acacctgccc gaacttcctg aacccctcgt
     2041 cgtccgagcg caggatctcg atcagccgcc cgcgctcgtc ggggatgaag cgcaggtcct
     2101 tgacccagac gccctcgatc cggtccgcca cgggccagag tctacaggag ggtcgctaca
     2161 ggacctccac ctgggactgg tcgccgagca tgaggcggtg ggcggcccga ccgcgcctcc
     2221 ccctcgagac ctcgacgtcc ttgcccagca gcgactccgc gatcccgggg accccgtgga
     2281 tccggcaccg ctccatcacg atcgagtcct cgacctcgct gccggtgatc tcgcagcccg
     2341 tgtagatcga ggcgtagggg ccgatcagcg aatcctcgat cgtggttccc tcgcccacga
     2401 tggaagggcc gcggatcacc gaacgtgtga ccttggcccc gggcgcgacc acgaccggac
     2461 cgacgaggcg cgacgcgtcg tccacctcgc cctccacccc gccctccaac cgggacagca
     2521 tcatccggtt ggcctcgagg aggtcctcga gcttccccgt gtccttccac cagccctcga
     2581 ccatctcggc ccgcacgttc tttccgtggt cgatcaggta ctggatcgag tcggtgatct
     2641 cgagctcgcc ccgccacgag ggctccacgc tggcaacggc ctcgtgcacg ctcgcgtcga
     2701 agagatagac gcccacgagc gccaggtcgc tgatccggtc cttcggcttc tccacgagcc
     2761 gcacgatccg gtcgccctcc agcacggcca cgccgaaccg ctcgggttcg gggaccttcg
     2821 ccaggaagat ctgggcgtcg ggcgcgtgct cctcgaactg ccgcacgaac cgcgagaccc
     2881 cctccagcag gatgttgtcc cccaggtaca tcacgaagtc gtcgtcgccg aggaagtcgc
     2941 gcgagatcag gacggcgtgg gccagcccca gcggcgcctc ctgagggatg taggtgatct
     3001 cgacgcccca gcgggagccg tcgccgcagg cctgctcgat ctcctcggcg gtgtccccca
     3061 ccacgatgcc gacctcgcgg atgccggcgt ccgccaccgc ctgcagccca tagaagagga
     3121 tcggcgtgtt ggccaccggg atcagctgct tcgagctggt gtgggtgatg ggtcggaggc
     3181 gggttccctc gccgccggcc aggatcaggg ccttcacggc gcatcctccc ccaggaccac
     3241 cgccacgtcc gcctccccga ggagggctcg tcctccctgg cgcagggtga ggtcggggaa
     3301 gtaccccgcc acgacctgag ccatcgcctc ggcccctgcg gcgtggacga tctcgctcgc
     3361 ctcgcggggc gggcggcggt cgccgggcag gacgatgaac ccggcgtccc tcagcaggtc
     3421 ctcggctcgg gcggcgaccg ccgcccgccc cgcccccacc accttcacgg cgatctgggc
     3481 cggcgacggc gggacctcgc ccgggggcgc cgtgccgagg tcgcctagcg gccggtcctc
     3541 acggagcctc cggaacagcg cctcggcctc gggcgtcatc tccacgaacg acgggccggc
     3601 caggccgggg acgatcctga ggtccaccgt ggaggcgccg gtcgggtcct cggcggccag
     3661 cccctgcagc tcccgtccgt agtcgagcag gtcggtgacg gtcagggcgt gatcggtccg
     3721 caccgagccg gccaccgccc tcaccagttc ggggatcctc gtgaccgatc ccagcgagaa
     3781 gagcttgttc gtcaccgccc tgatgaactg ctgctggcgg gagatcctgg cgaagtcggg
     3841 gatcaggtcc ccctccacgt tccgggcccg cacgaacgcc agcgccgtgt cgccgtccat
     3901 ctggtagcag ccggcctcgg gcagctcgag ctcggccatc gggtcgatca tgggccggtc
     3961 gatgcagacc tcgacgccgc ccagcgccgc gacgatcgtg cggaagctgt tgaagttcac
     4021 ctcgatgtag tggtggatgg ggatcccggt cagccggctc acggtcttga tcgcgagatt
     4081 ggggcccccc agcgtgaacg ccgcgttgat cttgtcctct ccgtgtcccg ggatctccac
     4141 gcgcaggtcg cgggggaagt ggacgagcac cgcgcgctcc tcctggggat cgaagtggcc
     4201 caggatgatc gtgtccgagc gctcgccctc caccatctcg ggcgagccga actgctcttc
     4261 ctcctcctcg agcccctcgc gcgtgtcgct tcccaggatc aggacgttga tcgggtcgag
     4321 cgggtcctcg gggacgtcga cgatatcggg cgtctgatcc tccgggattc ggttgacccg
     4381 ggagtccgcg taccagagga acccgagcgc cgccgacgac acggcgaacg cgacgagcgt
     4441 gaccaccccc accagcacca gccccacgtt ccgcagccgc cgctcggtga gcagacgccg
     4501 cttcccggta ggcttggtct cgctcacggc agtcaagcgt acccgggagg gcagatgagc
     4561 gacatcgtct gggagcccac cgaggagatc atcgagcgag cgaacgtcac ccgcttcatg
     4621 cgggcgcacg gcatcgagac ctacgaggag ctggtccgcc gctcgcggga cgacgtcgag
     4681 tggttctggg acgccgtcgt gaaggatctc gacatcccct tcctggaacc ctacgagcgc
     4741 gtgctcgaca ccagcgacgg gatcccctgg gcgaggtggt tcgtcggcgg gaagaccaac
     4801 ctcgcccacg ccacctgcga ccgctgggcc gaggagaccc ccgacgcggt ggcggtgctg
     4861 tgggagggcg aggacggccc cgtgcgctcg gtcacgtacc gggagctgcg cgagatggcc
     4921 gaccgcctgg cgaacgcgct ggcctcgctc ggggtcggtg agcgcgacgc cgtggggatc
     4981 ttcatgccga tggctcccga gacggtggcc gccgtgctgg cctgcgccaa gctgggggcc
     5041 gtctacctcc cgatcttctc cggctacgcg gccgacgcgg tggccacccg cctccgcgac
     5101 gccgacgcca aggtcctgct caccgccgac ggctttcctc ggcgaggctc ccaggtggcg
     5161 atgaaggaga tcgccgacga ggccgccgac gcctccccca ccgtggagaa ggtggtggtc
     5221 tggtcccgac tgggccgatc cgacctcccg tggcgagccg atcgagacct gagatgggag
     5281 gagctgaccg agaaggcatc tccctcgttc gagacgctct cgctcgacag cgagcacccc
     5341 ctcttcatcg cctacacgag cgggacgacc ggccgcccca agggctcggt ccacgtccac
     5401 ggcgggttca ccgtgaagat cgccgaggag gtcgcctacc agaccgacgt ccagcggggc
     5461 gacgtcctgt tctgggtctc ggacctgggc tggatcatgg ggccttggga gatcacgggg
     5521 gcgggcgcgc tgggggcaac ggtcttcctc tacgagggcg cccccaacca cccgcagccc
     5581 gaccggatct gggacatggt ggagcggcac cggattaccc acctcggcat ctcccccacc
     5641 ctgatccgag cgctgatccc ggccggcgac gagtgggtgg agcgccacga cctgtcctcg
     5701 ctccgcatcc tgggctccac cggcgagccc tggaaccccg acccctatct ctggttcctg
     5761 gagaaggtcg gcgggggccg ctgcccgatc atcaacctct cgggcggcac cgagatcggc
     5821 gcgtgcttcc tgtcaccact cccgatcaac gccctgaagc cggccaccct gcgcggcccg
     5881 gcgctgggga tggcgatgga cgtgtggggg gccgacggga agccggtcgc ccccggcgag
     5941 gtgggcgagc tcgtctgcac gaagccctgg ccggccatga cgcggggcgt ctggggcgac
     6001 ccccagcgct acctcgagac ctactggtcg cgctggcccg acgtctgggt ccacggcgac
     6061 tgggccagcg tggacgagga cgggttctgg ttcctccatg ggcgcagcga cgacaccatg
     6121 aacatcgc
//
LOCUS       DASUTW010000297         4383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1524567, whole genome shotgun sequence.
ACCESSION   DASUTW010000297 DASUTW010000000
VERSION     DASUTW010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524567"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VF058_10190"
     CDS             complement(<1..233)
                     /locus_tag="VF058_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX6208709.1"
                     /translation="MIAFVEGVLAERGADRVVVSAGGLGYELLVSTTTLAALPAVGKP
                     SRLLTHLQVRDDSMVLFGFSSGAERDLFELLITV"
     gene            complement(230..727)
                     /locus_tag="VF058_10195"
     CDS             complement(230..727)
                     /locus_tag="VF058_10195"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX6208710.1"
                     /translation="MFERVVLGVDPGVASTGLAAVGGGRRRAQVIWATTVRTPADAPE
                     AQRLRSIASAVREAIATHRPEALAIERLLWGRNTGSAMGVARASGAVMVAGAEAGIPV
                     HEYAPLEVKMAITGDGNAGKQQVRRSLARIHRVEGVPRQPDAADAAAVALCHLHRAPL
                     AEAVR"
     gene            complement(777..1616)
                     /locus_tag="VF058_10200"
     CDS             complement(777..1616)
                     /locus_tag="VF058_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208711.1"
                     /translation="MRARPIVVLLALGLGACTAPADPAPTAPSGPTYRDRVEVEAAVE
                     AFLEGFAEGGEHPDELLASVAGDRLRDWARWINRYRPEREAVRAGRVEIESLRVESIR
                     GGVAVAVVDATVHQPARDEVGARRIVARSFNGPVELRREASGWRVVDIVRDGAPMSYA
                     IGVFDEPAAVEEAGIRVEVSSVYRFRSGTVATIRIENGTGMPIVLDRTHSLIQAAGRW
                     VGATEVAGDLDVPIPPGVAIEGSLAYHAIPIQWLPEKVFVHFLEGPDPIEIDLPRDGF
                     LPI"
     gene            complement(1655..2401)
                     /locus_tag="VF058_10205"
     CDS             complement(1655..2401)
                     /locus_tag="VF058_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX6208712.1"
                     /translation="MSGHSKWSTIKHKKAAQDQRRGKLFSKLIRAIEVAAREGGGSVE
                     GNPTLASAVQKAKEFSVPNENIERAIKRGTGDLGGVRYEEVVYEGYGPGGVAVLVATL
                     TDNRNRTGSDVRSTFTKRGGNLGDPGSVSFLFERKGVIRVSREEADEDRLLELVLEAG
                     AEDLVEDGDEWEVRTGPTAFADVRTVVEGAGIPISSAEVTMLPQTTVPVEGSEAKQVL
                     GLIEALDDLDDVQAVYANFDIPEEMLAEVG"
     gene            complement(2401..2961)
                     /gene="pdxT"
                     /locus_tag="VF058_10210"
     CDS             complement(2401..2961)
                     /gene="pdxT"
                     /locus_tag="VF058_10210"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX6208713.1"
                     /translation="MKAGILALQGDFREHGRVIASCGATPVEVRTPEDLASVECLVIP
                     GGESTTIGTLAGGRGLVDAIRERAEGGMPVFGTCAGMIVMAKELIGGEPLLSLMDVTV
                     ERNAYGRQVDSFEAEVEVRGEPEPVRGVFIRAPVVVEVGEGVEILAEHEGRPVVVEQG
                     NLLAASFHPELAPDPRLHERLLGKVR"
     gene            3071..3451
                     /locus_tag="VF058_10215"
     CDS             3071..3451
                     /locus_tag="VF058_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6208714.1"
                     /translation="MLERYPVHPTLPAADIDRARAWYDEKLDLRPTSEDPGGLWYESG
                     GVVFALFPTPHAGSATNTAMEWRVDDVAAVVEWLQGRGVEFETFEMEGVEWDGPIAKM
                     GPARGAWFKDSEGNTLAVTEGLEG"
     gene            complement(3448..3978)
                     /locus_tag="VF058_10220"
     CDS             complement(3448..3978)
                     /locus_tag="VF058_10220"
                     /inference="COORDINATES: protein motif:HMM:NF039189.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HEX6208715.1"
                     /translation="MAEERRAEGRAAYVISQLYYYVAAVIGVALLIGGAVAALFGLRE
                     IVLPREWESTEGGIRTMLTGAAFALPGLALLWTHLREARRREGLPFSGTFWGASLYFH
                     LVALIALGFVLIGTTGILVSLVDVVYRPSAGPGLFRPSRSEALRQMLDFAIFVVVAGP
                     VFWWHLGRGRRAVGSP"
     gene            complement(3981..>4383)
                     /locus_tag="VF058_10225"
     CDS             complement(3981..>4383)
                     /locus_tag="VF058_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX6208716.1"
                     /translation="RHVRTITGQMSRLRGMRDDELMSQAKELGAPFELVRQVASTGTL
                     PVVNFAAGGLATPADAALMMQLGVEGVFVGSGIFKSDDPATRARAIVEATTSYRDPEM
                     LAKISRGLGEPMKGIEISTLGEEGLLQTRGW"
BASE COUNT          635 a         1655 c         1502 g          591 t
ORIGIN      
        1 accgtgatca gcagctcgaa gaggtcgcgc tccgcgcccg aggagaagcc gaacagcacc
       61 atcgagtcgt cacggacctg gaggtgggtc aggagtcgcg agggcttccc gaccgccggc
      121 agcgccgcca gcgtcgtcgt cgagacaagg agctcgtatc ccagcccgcc cgcgctcacg
      181 accacccggt cggcgccccg ctcggccagg acgccctcca cgaacgcgat caccggacgg
      241 cctccgccag cggcgcacgg tgcaggtggc acagggccac ggccgcggcg tcggcggcgt
      301 cgggctggcg cgggacgccc tccacccgat ggatcctcgc cagggagcgg cggacctgct
      361 gcttccccgc gttcccgtcg ccggtgatcg ccatcttcac ctccagcggt gcgtactcgt
      421 ggaccgggat gcccgcttcc gcgccggcga ccatcacggc cccgctggcc cgggccaccc
      481 ccatcgcgct gccggtgttg cgcccccaga ggaggcgctc gatcgcgagc gcctccggcc
      541 ggtgcgtcgc gatcgcctct cggacggcgc tcgcgatcga gcgcaagcgc tgggcctcgg
      601 gcgcgtctgc cggcgtgcgc acggtcgtcg cccagatcac ctgcgcccgg cgccgacccc
      661 cgcccacggc ggcgaggccc gtggacgcca cacccgggtc cacgccgagc accacccgct
      721 cgaacacgtg ttcagtctag gggggggagg cggcccgctc aaggaggcgg cgggattcag
      781 atcgggagga agccgtctcg gggaaggtcg atctcgatcg gatcggggcc ctcgaggaag
      841 tgcacgaaca ccttctcggg cagccactgg atcgggatcg cgtggtaggc cagggagccc
      901 tcgatcgcga ccccgggcgg gatcgggacg tccaggtccc cggcgacctc cgtggcgccc
      961 acccatctcc ccgccgcctg gatcagggag tgggttcggt ccaggacgat cggcatcccc
     1021 gtgccgttct cgatccggat cgtggccacg gtccccgacc ggaagcggta cacgctggag
     1081 acctccacgc ggatgccggc ctcctccacc gccgccggct cgtcgaagac gccgatcgcg
     1141 tagctcatcg gcgcgccgtc ccggacgatg tccaccactc gccaccccga tgcctcgcgc
     1201 cgcagctcca ccgggccgtt gaacgaccgg gccacgatcc ggcgcgcccc cacctcgtcc
     1261 cgggccggct ggtgcaccgt cgcatccacg accgcgaccg ccaccccgcc ccggatcgac
     1321 tcgacgcgca ggctctcgat ctcgacgcgg cccgctctca cggcctcccg ctcgggccgg
     1381 tagcggttga tccagcgggc ccagtcccgg agacggtcgc ccgcgaccga ggccaggagc
     1441 tcgtcgggat gctcgccgcc ctccgcgaat ccctcgagga aggcctcgac cgcggcctcg
     1501 acctcgaccc gatctcggta ggtggggccg ctcggggcgg tgggggcggg atcggcgggc
     1561 gccgtgcagg cgccgagacc gagcgccagc agcacgacga tgggtcgagc ccgcatggct
     1621 cgagtatccc ccggagcgcg gctcttggcc cgggtcagcc gacctcggcc agcatctcct
     1681 cggggatgtc gaagttcgcg tagacggcct ggacgtcgtc gagatcgtcc agcgcctcga
     1741 tcagccccag cacctgcttc gcctcggatc cctccacggg gaccgtcgtc tgggggagca
     1801 tcgtgacctc cgccgaggag atggggatgc ccgcaccctc gacgacggtc cgcacgtcgg
     1861 cgaaggccgt cgggccggtg cgcacctccc actcgtcgcc gtcctcgacc aggtcctcgg
     1921 cgccggcttc cagcacgagc tccaagaggc ggtcctcgtc cgcctcctcc cgggacacgc
     1981 ggatcacgcc cttccgctcg aagaggaacg agacgcttcc cgggtccccc aggttcccgc
     2041 cgcgcttcgt gaacgtggag cggacgtccg agcccgtcct gttgcggttg tccgtcagcg
     2101 tggccacgag gaccgcgacc ccgccgggtc cgtacccctc gtagacgacc tcctcgtagc
     2161 ggacgccccc gagatcgccg gtcccccgct tgatcgcccg ctcgatgttc tcgttcggga
     2221 cggagaattc cttggccttc tggaccgccg aggccagggt cgggtttccc tcgacgctgc
     2281 ctccgccctc gcgcgcggcg acctcgatcg cccggatcag cttggagaag agcttccctc
     2341 ggcgctggtc ctgcgccgcc ttcttgtgct tgatcgtgga ccacttggag tgcccggaca
     2401 tcagcgcacc tttcccagca gccgctcgtg cagccgcggg tccggggcga gctcggggtg
     2461 gaaggacgcc gccagcaggt tcccctgctc cacgacgacc gggcgcccct cgtgctcggc
     2521 gaggatctcc acgccctccc ccacctcgac caccaccggg gccctgatga agacgccgcg
     2581 caccggctcg ggctcgccgc gcacctcgac ctcggcctcg aacgagtcca cctgccgccc
     2641 gtaggcgttg cgctcgacgg tgacgtccat cagcgagagg agtggctccc ccccgatcag
     2701 ctccttggcc atcacgatca tcccggcgca cgtcccgaag accggcatgc ccccctcggc
     2761 ccgctccctg atcgcgtcca cgagcccccg tcccccggcg agcgtgccga tcgtggtgga
     2821 ctccccgccg gggatcacca ggcactccac cgaggcgaga tcctcgggcg ttcggacctc
     2881 gacgggggtc gcgccgcagc tcgcgatgac ccggccgtgc tcccggaaat cgccctggag
     2941 ggcgaggatg cctgccttca tgtcatcgcc atgatagcga cgcaggcggg gggcttccca
     3001 agcgcctcgg gttggagcag gatgtcgacg atgcgagcga gcgctcgtgc ccgcggaagg
     3061 aggtgcggag atgcttgaac ggtacccggt gcacccgacg ctcccagccg cggacatcga
     3121 ccgggcgcgg gcctggtacg acgagaagct cgacctgcga cccacgagcg aggaccccgg
     3181 aggcctctgg tacgagtccg ggggcgtggt cttcgcgctg ttccccacgc cccatgcggg
     3241 cagcgccacg aacaccgcga tggagtggcg ggtggacgac gtggccgcgg tcgtcgagtg
     3301 gctccagggc cgcggggtgg agttcgagac cttcgagatg gagggcgtcg agtgggacgg
     3361 tccgatcgcg aagatggggc ccgcccgcgg cgcctggttc aaggacagcg aagggaacac
     3421 cctggcggtg acggagggcc tggagggcta gggggacccg acggcgcgcc ggccccgccc
     3481 gaggtgccac cagaagacgg ggccggccac gaccacgaag atcgcgaagt ctagcatctg
     3541 ccggagcgcc tccgaccggg agggccggaa caggcccggt cccgcggagg gccgatagac
     3601 gacgtccacg agcgagacca ggatcccggt cgtcccgatc agcacgaatc ccagcgcgat
     3661 cagcgcgacg aggtggaagt agagggacgc gccccagaac gtcccggaga aaggcagtcc
     3721 ctcgcgccgc ctggcctctc ggagatgggt ccagagcagc gccagcccgg gcagggcgaa
     3781 ggcggcgccg gtgagcatcg ttcggatgcc gccctcggtg gactcccact cccgaggaag
     3841 gacgatctcc cggagcccga agagggccgc caccgcgccg ccgatgagga gcgccacccc
     3901 gatcacggcg gcgacgtagt agtagagctg ggagatgacg taggcggccc tgccctcggc
     3961 ccgccgctcc tccgccatcc ctaccagccg cgggtctgga ggagaccctc ctcgcccagc
     4021 gtggagatct cgatcccctt catcggctct ccgagacctc gagagatctt cgcgagcatc
     4081 tcggggtccc ggtaggaggt cgtggcctcc acgatggccc gggccctggt ggccgggtcg
     4141 tctgacttga agatcccgga tcccacgaag acgccctcca ccccgagctg catcatgagg
     4201 gccgcatccg cgggcgtcgc caggcccccg gccgcgaagt tcacgaccgg cagggtcccg
     4261 gtgctcgcga cctgccgcac gagctcgaag ggggccccca gctccttggc ctgggacatc
     4321 agctcgtcgt cgcgcatccc ccgcagccgc gacatctgcc cggtgatcgt gcggacgtgg
     4381 cgg
//
LOCUS       DASUTW010000298         3961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1913317, whole genome shotgun sequence.
ACCESSION   DASUTW010000298 DASUTW010000000
VERSION     DASUTW010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1913317"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1431
                     /gene="ligD"
                     /locus_tag="VF058_10230"
     CDS             <1..1431
                     /gene="ligD"
                     /locus_tag="VF058_10230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX6208717.1"
                     /translation="GDAAARLAKYGEMRDFAETPEPPPVPAGGDGRSFVIQKHEATRL
                     HYDLRLEEGGVLASWAVPRGLPLTPGDRRLAVHTEDHPLDYGGFQGWIPEGHYGAGEV
                     RIFDRGTYEPLEWTDEKVSFRLHGTRHRGEYHLVKTDRDWLIFLGKASDAPQPEPPPA
                     LQPMMADPGHDPFDDPGWRFEPKLDGVRTLAYVTTDATRLLSRKGRDQTAQYPELDNL
                     ARWTNALWAVLDGEIVAVDDAGRPSFERLQERINLSAPSDIEKARRRIPVRLYAFDLL
                     WLDERDLTNEPLEERRRLLEGIVTESDRLGLTLYLDGEGERLFEAAKGLGYEGIVAKK
                     LGTRYQPGRRSKDWRKVKAVKRQDCVILGWTRGEGSRASSFGALLLGAYVGDELRWVG
                     QVGTGFTEQLTLQVLDRLREIREEEPPIGDRELASVKGATWVRPELVCEVEYLHVTKA
                     GKLRAPSFKGIRPDKAPQDCILEEAP"
     gene            1428..1889
                     /locus_tag="VF058_10235"
     CDS             1428..1889
                     /locus_tag="VF058_10235"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6208718.1"
                     /translation="MTLNRYRFRSVWRIPATPEVVLELLHELDAWSRWWPEIRESHRV
                     DEDRAVVRIRSALPYDLHLLLTRRHDTESILEAGIEGDLRGFARFRLRPDGDGSTRLL
                     FEQDVKARKPLMRRLAPIARPLFRWNHARMMRSGRRGLAELVATGTGRSRP"
     gene            complement(1861..2367)
                     /locus_tag="VF058_10240"
     CDS             complement(1861..2367)
                     /locus_tag="VF058_10240"
                     /inference="COORDINATES: protein motif:HMM:NF036411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5319 family protein"
                     /protein_id="HEX6208719.1"
                     /translation="MADEEPRPLDPDERRNIEADLEDLSAMRSVFEGQGMRGVAIACP
                     ECQTDHFYGWDLIIESLEHMLETGEPRMHEPAFDPREEDYVLWDYGKGYVDALADAGL
                     KETPGSEVTLLACAWCDTPVEAHFSYCPKCGRSMAPARLLRELVSRGMDEREARALLV
                     KAGFDPFR"
     gene            complement(2370..3074)
                     /locus_tag="VF058_10245"
     CDS             complement(2370..3074)
                     /locus_tag="VF058_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX6208720.1"
                     /translation="MEIERALVAFAHPDDAEFLCGGTVALWAKQGVEVTYVCATDGSA
                     GWNAPGMTREEIAARREEEMREVADLLGVKEVRFLGYPDGMLEPSLDLRRSLTREVRR
                     ARPDILVSFDPSELWSGRSYINHPDHRAVGEAVLAVTACDAPTRPQFPELLEEGLEPF
                     KVRGVWLASDEGEHVVDIGEAIDLKIQAVRAHQSQMENMGEFDADARVRRWARDQAEG
                     TPHEFVERFRAFRIEV"
     gene            complement(3102..3431)
                     /locus_tag="VF058_10250"
     CDS             complement(3102..3431)
                     /locus_tag="VF058_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208721.1"
                     /translation="MSFEDALRDLGFVFSRRGRGGETWEHRATPYLTHYVQLLEDGTA
                     LFTWEVAIGELMAAHGLQVGSDEHLNTFLFPREDARGPRDAAWVSAQMDAVEERLTAI
                     SLLSAQP"
     gene            complement(3454..>3961)
                     /locus_tag="VF058_10255"
     CDS             complement(3454..>3961)
                     /locus_tag="VF058_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208722.1"
                     /translation="AAAAEVEALEAEEDPAAREPIARRLEDEVAKRAREVASYLEPSL
                     TTPFAVACVPDAAYAACRKAHAVAFRHGVVIVAYSAALPVLLSLYALGSRYGQSGDVE
                     ACLAELESVLEGMEQTLENRVARATTMLQNAADEWRAGLGRARWAVAKGRGTAGALED
                     ERRLEAVE"
BASE COUNT          561 a         1471 c         1387 g          542 t
ORIGIN      
        1 ggggacgccg cggcccgcct cgccaagtac ggcgagatgc gcgacttcgc ggagacgccc
       61 gagcccccgc ccgtccccgc cggcggcgac gggcgctcct tcgtgatcca gaagcacgaa
      121 gccacccggc tccactacga cctgcggctg gaggagggcg gggtgctggc ctcgtgggcc
      181 gtcccgcgcg ggcttcccct gacgcccggc gaccgtcgcc tcgccgtcca caccgaggac
      241 caccccctcg actacggggg attccaagga tggatccccg agggccacta cggcgcgggg
      301 gaggtccgca tcttcgaccg tggcacctac gagccgctgg agtggaccga cgagaaggtc
      361 tcgttccgcc tccacgggac ccggcatcgc ggcgagtacc acctcgtgaa gaccgaccgg
      421 gactggctga tcttcctggg caaggcgtcg gatgcgcccc agcccgagcc gccgcccgcg
      481 ctccagccga tgatggccga cccggggcac gatccgttcg acgacccggg ctggcgcttc
      541 gagccgaagc tcgacggcgt ccggacgctc gcctacgtca ccaccgacgc cacgcggctc
      601 ctctcgcgga agggccgcga ccagacggcc cagtaccccg agctcgacaa cctggcccgg
      661 tggacgaacg cgctgtgggc cgtcctcgac ggcgagatcg tggccgtgga cgatgcgggg
      721 cgcccctcgt tcgagcggct ccaggagcgg atcaacctct ccgctccgtc cgacatcgag
      781 aaggcccgcc gccgcatccc cgtgcggctg tacgccttcg acctgctgtg gctcgacgag
      841 cgagacctga cgaacgagcc gctggaggag cgccgccgcc tgctcgaggg catcgtcacc
      901 gagtcggatc gtctggggct caccttgtac ctggacgggg agggcgagcg gctgttcgag
      961 gcggccaagg gcctcggcta cgagggcatc gtggccaaga agctcggcac ccgctaccag
     1021 cccggccggc ggtcgaagga ctggcggaag gtgaaggcgg tgaagcggca ggactgcgtg
     1081 atcctcggct ggacccgggg cgagggctcg cgggcgtcgt cgttcggagc gctgctgctc
     1141 ggggcctacg tgggtgacga gctccgctgg gtgggccagg tggggacggg cttcaccgag
     1201 cagctgaccc tccaggtcct ggaccgcctc cgcgagatcc gcgaggagga gcccccgatc
     1261 ggcgaccgcg agctggcctc ggtgaagggc gcgacctggg tgcggcccga gctcgtctgc
     1321 gaggtcgagt acctgcacgt gacgaaggcc gggaagctgc gcgccccctc gttcaagggc
     1381 atccgccccg acaaggcccc acaggactgc atcctcgagg aggccccctg accctcaacc
     1441 ggtaccgctt tcgctcggtc tggcgcatcc ccgcgacgcc cgaggtcgtc ctcgagctcc
     1501 tccacgagct cgacgcctgg agccggtggt ggcccgagat ccgggagagc caccgcgtgg
     1561 acgaggaccg cgcggtcgtc cgcatccgct ccgcgctccc ctacgacctg cacctcctgc
     1621 tgacccgccg ccacgacacc gagagcatcc tcgaggcggg gatcgagggg gacctccgcg
     1681 gcttcgccag gttccgcctg cgtcccgacg gcgacggctc gacccggctc ctcttcgagc
     1741 aggacgtcaa ggcgaggaag ccgctgatgc gaaggctcgc cccgatcgcc cgtccgctct
     1801 tccgctggaa ccacgcgagg atgatgcgct cggggcggcg cgggctcgcg gagctcgtcg
     1861 ctaccggaac gggtcgaagc cggccttgac cagcagggcc cgggcctcgc gctcgtccat
     1921 ccctcgactc acgagctcgc ggaggagacg ggccggggcc atggagcggc cgcacttcgg
     1981 gcagtacgag aagtgcgcct ccaccggggt gtcgcaccag gcgcaggcca ggagggtcac
     2041 ctcggagccg ggggtctcct tgaggccggc gtcggccagc gcgtccacgt accccttgcc
     2101 gtagtcccag aggacgtagt cctcctcgcg gggatcgaac gcgggctcgt gcatccgggg
     2161 ctcgccggtc tcgagcatgt gctccaggga ctcgatgatg aggtcccacc cgtagaaatg
     2221 gtcggtctgg cattcggggc aggcgatggc gacgccccgc atgccctgcc cctcgaagac
     2281 cgaccgcatc gccgataggt cctcgaggtc ggcctcgatg ttccggcgct cgtcgggatc
     2341 cagggggcgt ggttcctcgt cggccatcct cagacctcga tccggaacgc gcggaaccgc
     2401 tccacgaact cgtggggcgt gccctccgcc tggtcgcggg cccacctccg gacgcgggcg
     2461 tcggcgtcga actcgcccat gttctccatc tggctctggt gggcccgcac cgcctggatc
     2521 ttcaggtcga tcgcctcgcc gatgtccacc acgtgctcgc cctcgtcgga ggcgagccac
     2581 acgccgcgga ccttgaacgg ctcgaggccc tcctccagaa gctcggggaa ctgggggcgc
     2641 gtcggcgcgt cgcatgccgt gacggccaac acggcctccc ccaccgcccg gtggtcgggg
     2701 tggttgatgt agctccggcc gctccacagc tccgaggggt cgaacgagac gaggatgtcg
     2761 gggcgggccc ggcgcacctc tcgggtcagg gagcgccgca ggtcgagcga gggctccagc
     2821 atcccgtccg gatatcccag gaagcggacc tccttcaccc cgaggaggtc ggccacctcg
     2881 cgcatctcct cctcgcggcg cgcggcgatc tcctcgcggg tcatgccggg cgcgttccag
     2941 ccggccgagc cgtcggtggc gcagacgtac gtgacctcga cgccctgctt ggcccagagc
     3001 gccaccgtcc ctccgcagag gaactcggcg tcgtccgggt gggcgaaggc gacgagggcg
     3061 cgctcgatct ccacgcgcca agcgtagcgc gagcgaccgg ctcagggctg ggccgagagc
     3121 agtgagatgg ccgtcaggcg ctcctcgacg gcgtccatct gggccgagac ccatgccgcg
     3181 tcgcgggggc cccgggcgtc ctcgcgtggg aacaagaacg tgttgaggtg ctcatcggag
     3241 ccaacctgga gtccgtgggc cgccatcagc tccccgatcg cgacctccca ggtgaagagc
     3301 gctgtgccgt cctcgagcag ctgcacgtag tgggtgaggt acggcgtggc acggtgctcc
     3361 caggtctcgc cgcccctacc ccgccgggag aagacgaacc ccagatcccg cagcgcgtcc
     3421 tcgaacgaca cggctcgcag gatatcgtcg ccgctactcc accgcctcga gccgccgctc
     3481 gtcctcgagg gctcccgcgg tcccccgccc cttggccacc gcccagcgag cccgacccag
     3541 cccggcccgc cactcgtcgg cggcgttctg gagcatcgtc gtggcgcgag cgacccggtt
     3601 ctccagggtc tgttccatgc cctcgagcac gctctccagc tccgccaggc aggcctcgac
     3661 gtcgccgctc tgcccgtacc gcgacccgag cgcgtacagg gagagcagga ccggcagcgc
     3721 cgcagagtag gccacgatga ccaccccgtg ccggaaggcg accgcgtgcg ccttccggca
     3781 cgccgcgtag gccgcgtcgg ggacgcaggc cacggcgaag ggggtggtca gcgacggctc
     3841 gaggtacgac gcgacctcgc gcgcccgctt cgccacctcg tcctcgagcc gacgggcgat
     3901 gggctcgcgc gcggcggggt cctcctcggc ctccagggcc tcgacctccg cggcggcggc
     3961 c
//
LOCUS       DASUTW010000299         2784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_226706, whole genome shotgun sequence.
ACCESSION   DASUTW010000299 DASUTW010000000
VERSION     DASUTW010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226706"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..278)
                     /locus_tag="VF058_10260"
     CDS             complement(27..278)
                     /locus_tag="VF058_10260"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX6208723.1"
                     /translation="MLARSVGESVILAAGDREGFEVLDGAGAIIWDLLEEEVTSGQIT
                     ATLAAWFEGPRDVIARDVEGLLADLRQRGLIEEVDASRG"
     gene            complement(311..1621)
                     /locus_tag="VF058_10265"
     CDS             complement(311..1621)
                     /locus_tag="VF058_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX6208724.1"
                     /translation="MMKNTARLRRQILSREASVAVVGQGYVGLSVACAAAEAGFTVTG
                     IDSDHERVEDLSGGILSVAGVSDEVFRAAMATGRLGFSTEHAALADHGVILICVPTPV
                     RDSTPDVSFIENACEKVAADLGPGCLVVLESTTYPGTTEELVRPILETSGFFADRDFL
                     LAYSPERIDPGNEEYGMRNIPRVVGGVTPEAAGLATLFYEQLVDKVFAVSSTRAAELA
                     KLLENTFRHVNIALVNEMATLCHEMGIDVWEVIEATATKPFGFMPFSPGPGVGGHCIP
                     LDPTFLAWQVRRDAGHQFRILEQAEDVNAQMPRYVASRIGEALNEEGKPVRQSSVLVL
                     GVAYKPDVGDVRESPALGVMRHLVRTKAKLSFHDPYVESVTLGGDVLRRTELTLRSVS
                     AADCVALLTPHSAYDLEWIAEHARLIFDAQNAFGASVPPNVVRL"
     gene            complement(1649..2008)
                     /locus_tag="VF058_10270"
     CDS             complement(1649..2008)
                     /locus_tag="VF058_10270"
                     /inference="COORDINATES: protein motif:HMM:NF019021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX6208725.1"
                     /translation="MVDSVLDSPRDVVRCLITYADWWQPATSSILQVGAARRGGDRND
                     GMRAGTLETLDERTELRRRVDRLDDRERKLLFLWYVTELPVGEIARSVGVSRRHCFRV
                     RSRAIHKIVEMADDPRA"
     gene            complement(2056..>2784)
                     /locus_tag="VF058_10275"
     CDS             complement(2056..>2784)
                     /locus_tag="VF058_10275"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEX6208726.1"
                     /translation="VSPAAEAVRLLAVGVEHGPATAWTERGNGARGRHGGLKVLLVTS
                     PAPSEGKTSIVANLAAAYAEMGKRVLILSCDMRHPGTYSFFGMPSANGVGMSDALKGD
                     GEEVLSDYVRSTGHRRIEIVPSGSPTDRPGELLAGPNMRRALSEARERADLVLIDTPP
                     ILTAGDATHLLPYVDAVLLVARAGRTSARLAERTSEILKRLEVPAVGVVLNGVKDAAF
                     ARGYYRYAAKGDGAKNVPQPSPWE"
BASE COUNT          444 a          998 c          922 g          420 t
ORIGIN      
        1 ggaggaaggg ctctaccggc cgccagtcag ccacggctcg cgtcgacctc ctcgatgaga
       61 cctcgctgcc tcagatcggc caacagcccc tcgacgtccc gcgcgatcac gtcgcgcgga
      121 ccctcgaacc atgccgcgag cgtagccgtg atctggcccg acgtgacctc ctcctccagg
      181 aggtcccaga tgatggcacc tgccccgtcg agcacctcga agccctctcg gtcccccgca
      241 gcgaggatca cgctctcgcc gaccgatcga gcgagcacgg ccggagatcg ccggtagcga
      301 ccctccgcgc tcacaaccga accacgttgg gcggcacgct cgccccgaag gcgttctgcg
      361 cgtcgaagat gagccgagcg tgctccgcga tccattcaag gtcgtacgcg ctgtggggtg
      421 tgagcagcgc cacgcagtcg gccgcggaga cgctgcgcag ggtgagctcc gtccgtcgga
      481 gcacgtcgcc ccccagggtg acggactcca cataggggtc gtggaacgag agcttggcct
      541 tcgtcctgac gagatgcctc atcacgccca acgccggaga ctctcgcacg tcccccacgt
      601 cgggcttgta ggcgacgccc aggaccagga cgctcgactg gcgcacgggc tttccctcct
      661 cgttgagggc ctccccgatc cggctcgcga cgtacctggg catctgggcg ttgacatcct
      721 cggcctgctc gagtatccgg aactggtggc cggcgtcgcg gcgcacctgc cacgccagga
      781 aggtcgggtc gagcgggatg cagtgcccgc cgacgccggg gccgggcgag aagggcatga
      841 aaccgaaagg cttcgtggcc gtggcctcga tcacctccca gacgtcgatc cccatctcgt
      901 gacagagcgt cgccatctcg ttgaccaggg cgatgttcac gtgccggaag gtgttctcga
      961 ggagcttcgc cagctcggcg gcccgcgtgg acgagaccgc gaagaccttg tccacgagct
     1021 gctcgtagaa cagcgtggcc agtcccgccg cctccggggt gacacctccg acgacgcgcg
     1081 ggatgttccg catcccgtac tcctcgttcc ccgggtcgat ccgctcgggc gagtaggcga
     1141 ggaggaagtc ccggtcggcg aagaaccccg aggtctccag gatgggtctc acgagctcct
     1201 cggtggtccc cggatacgtc gtggactcga gcacgacgag gcagccgggg ccgaggtcgg
     1261 ccgccacttt ctcgcaggcg ttctcgatga acgagacgtc cggggtgctg tcccgcacgg
     1321 gggtcggcac gcagatcagg atcaccccgt ggtcggcgag cgcggcgtgc tccgtcgaga
     1381 agccgaggcg gccggtggcc atcgccgcgc ggaacacctc atcggacacc ccagccaccg
     1441 acaggatgcc gccggagagg tcctcgaccc tctcgtggtc ggagtcgatc ccggtcacgg
     1501 tgaacccggc ctccgcggcg gcgcaggcca ccgagaggcc gacgtagccc tggcccacga
     1561 cggcgacgga ggcctcgcgg gacaggatct gacgacgtag ccgtgcagtg ttcttcatca
     1621 tcgatctcca gacagaaggg ggtgatcctc aggcccgagg gtcgtcggcc atctcgacga
     1681 tcttgtggat cgcccgcgac cggacccgga agcagtgccg acgggacaca ccgaccgacc
     1741 gcgcgatctc gcccaccggg agctcggtca cgtaccagag gaagagcagc ttccgctcac
     1801 gatcgtccag ccggtcgacc ctgcggcgaa gctccgtacg ctcgtcgagc gtctcgaggg
     1861 tcccagcgcg catcccgtcg ttccggtctc cgccccgacg cgccgccccc acctggagga
     1921 tcgacgacgt ggccggctgc caccagtccg cgtaggtgat gagacacctg acgacgtcgc
     1981 gcggagaatc aagcacagaa tccaccactc aagaggatac ggggctcccc ggtgacacgg
     2041 tagatgggga aatccctact cccaggggct gggctggggg acgttctttg ccccgtcccc
     2101 cttcgccgcg tagcggtagt agccgcgggc gaacgcggcg tccttgaccc cgttcaggac
     2161 caccccgacc gccggcacct cgaggcgctt caggatctcg ctcgtccgct ccgcaagccg
     2221 ggcgctcgtc ctgccagccc tcgccacgag gagcacagcg tccacgtagg gcagcagatg
     2281 cgtcgcgtcg ccggcggtga ggatgggcgg cgtgtcgatc agcaccagat ccgcccgctc
     2341 ccgggcttcc gacagcgccc gtcgcatgtt cgggccggcc agcagctcgc cgggccgatc
     2401 cgtgggagat ccgcttggca cgatctcgat cctccggtgg cccgtggatc gcacgtagtc
     2461 ggaaaggacc tcttctccgt cccccttgag cgcgtcgctc atcccgacgc cgttggccga
     2521 tggcataccg aagaagctgt acgtcccggg gtggcgcatg tcgcaggaga ggatgagcac
     2581 ccgcttcccc atctcggcat aggccgccgc gaggttggcc acgatgctgg tctttccctc
     2641 ggaaggagcg ggacttgtga cgaggaggac cttgaggccc ccgtgtcgcc ccctggcgcc
     2701 gttgccccgt tccgtccacg cagtcgccgg accgtgctcc acgcccactg cgaggagacg
     2761 gacggcctct gcggccggcg agac
//
LOCUS       DASUTW010000300         6285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1526274, whole genome shotgun sequence.
ACCESSION   DASUTW010000300 DASUTW010000000
VERSION     DASUTW010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526274"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VF058_10280"
     CDS             complement(<1..712)
                     /locus_tag="VF058_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208727.1"
                     /translation="MRGFACLIVGSLLVSVAPLAQAAPAKTRRVNETSAGQPADSWSY
                     GSEISPNGRWVVFTSESTNLPGATGTHALVYVHDRETKKTRLVSKNSAGEPANEWSFD
                     GSISDDGRFVAFTSGATNLPGGTGTYELVYVHDRKTKKTRLVSKTSSGEPADSWSGES
                     SVSSNGRYVAFDSEASNLPGGAAHTGNMVYVHDRKAKKTRLVSKTSAGAPADDDSDDG
                     SISPDGRFVAFESDASNLP"
     gene            complement(815..1786)
                     /gene="hemB"
                     /locus_tag="VF058_10285"
     CDS             complement(815..1786)
                     /gene="hemB"
                     /locus_tag="VF058_10285"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017603124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX6208728.1"
                     /translation="MTFPQDRPRRLRRTTALRDLVRETDLAPRHLVAPLFVKEGIAEP
                     APISSMPGHHQHTLESLRKEARELAERGIRAFVLFGIPERKDAEGGEAWAEEGISQHA
                     VRALREELGEEVVLIADLCLCEYTDHGHCGVLDGEELDNDATLERYRRIALAQAAAGA
                     DLVAPSGMMDGQVAAIREALDEGGFPGTAILAYAAKYASSFYGPFREAAEGAPRFGDR
                     RSHQMDPGNADEALREIRADVAEGADLVMVKPALPYLDVIRRARDAVDVPVAAYNVSG
                     EYAMIKAAAERGWIDGTAAMLETLTAIRRAGAGLILTYFAREAADVL"
     gene            complement(1812..2567)
                     /locus_tag="VF058_10290"
     CDS             complement(1812..2567)
                     /locus_tag="VF058_10290"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX6208729.1"
                     /translation="MTALEGRTVVVTRSADRVEDLAALLRERGAVPLPAPAIRIEPVP
                     EGGPLDEAITDAAMRGYAWVVFTSGAGVAAGFARAAELGVDARALNAKVAAVGSGTAE
                     RLGDHGIEPELVPPEFTTASLAETFPAGSEKVLLARADIATPELEESLRGKGWQVTRV
                     DAYRTIPAHELPEAVAQALGEGRVDAVTFTSRSTVQGFTALAEVPAGVLVACIGPVTA
                     DAARAAGLRVDAVASPHTLEGLVEALERAFASR"
     gene            complement(2564..3451)
                     /gene="hemC"
                     /locus_tag="VF058_10295"
     CDS             complement(2564..3451)
                     /gene="hemC"
                     /locus_tag="VF058_10295"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX6208730.1"
                     /translation="MRIRIGTRRSRLALAQASEVAELLGGSGHEAELVPMTTAGDRGA
                     PAEASPQGTKGLFVAEITEALLAGGVDLAVHSAKDLPSDDPEGVVVAAVPRRSLPYDV
                     LVTATAGFDEEGTVGTSSLRRQAQLLRAYPSARVVPFRGNVDTRLRKLRQGEVDAVVL
                     AAAGLDRLGVRPAHAEPIPVDVMVPAPGQGALAVQARAEDPVAEAVATLDHASTRRAF
                     EVERALVGALGGGCRLPLGAYAEARSGTLRLLAVVARPDGSELLRAEVEGDDPGEVAA
                     HAAEQLRAAGADAILREAS"
     gene            complement(3451..3546)
                     /locus_tag="VF058_10300"
     CDS             complement(3451..3546)
                     /locus_tag="VF058_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208731.1"
                     /translation="MVGLKERWGAGVGEAHARVLAELFGLEPPKD"
     gene            complement(3582..4715)
                     /gene="hemA"
                     /locus_tag="VF058_10305"
     CDS             complement(3582..4715)
                     /gene="hemA"
                     /locus_tag="VF058_10305"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX6208732.1"
                     /translation="MPILALGVSYRRASVDVLERLAWGDEDLPKAYDHVRSMPSVEEA
                     VLLSTCNRVEVYAAVEGYHTGFQDLKRFLSESGGVEPEEFAEPLYSHYEDHTAEHLFS
                     VAAGLDSMVVGEPQILTQVRQAFQRARGEEAAGTQLSALFRYAIRAGRRARAETKIGA
                     SPSAFVEAGGEAAARHLGGLGGRSVLLVGAGEMAALALGWLRRRGVAEVTVLNRTPEK
                     AERLAARVGGSHGGLDELSDALARADLVVSSTTATQVVVGREDVERAAGGRRVFLLDL
                     AVPRDVDPAAREVPGVRVADIDDLRDVVAASREGIADEIEAAGRVIAEETRKFVTQRR
                     AAKLAPLIAALHERGEEVRAAELERLASRLSGLSDRERQAVRP"
     gene            complement(4728..5480)
                     /locus_tag="VF058_10310"
     CDS             complement(4728..5480)
                     /locus_tag="VF058_10310"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX6208733.1"
                     /translation="MENTPVPSCRARSRTHERPMTKRPIPEATVGRLPVYLRVLVEMA
                     EGGRSTVSSEALAEAAGVNSAKVRKDLSYLGSYGTRGVGYDVAYLIHQVRRELGLTQD
                     WPVLIAGVGNLGQALANYRGFDARGFRVAGLVDPARVGERVAGVEVRPPEEMPDLVRE
                     HGIQIGMICTPGPAAQEVADRMVEAGIRSILNFSPVVIACPEGVSLRKVDLSVELQIL
                     AFYEQRKAVLDEVRRGEALPQALGETAERPTA"
     gene            complement(5490..5723)
                     /locus_tag="VF058_10315"
     CDS             complement(5490..5723)
                     /locus_tag="VF058_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX6208734.1"
                     /translation="MAEVVMYARPGCHLCDEAREVILGLRAELGFDYSEVDVEGDAEL
                     EREHGLRVPVVAVDGEEAFEYRVDVDELRRLLS"
     gene            5767..>6285
                     /locus_tag="VF058_10320"
     CDS             5767..>6285
                     /locus_tag="VF058_10320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6208735.1"
                     /translation="MAEADDELARRVADRDPEAYEQIYRRYAPAAYGVALRVLRDPAL
                     AQEVAAESFLALWSTPEAYDASRGRFRSFFLSLVHHRAVDAVRREERLRERERKVNRA
                     PLPDEDWSEAVVEEADLIDRRREVREALSELPPEQREALELMYFRGYTQSRIAEERGI
                     PLGTVKTRVHAAM"
BASE COUNT          833 a         2426 c         2186 g          840 t
ORIGIN      
        1 cggggaggtt cgaggcgtcg gactcgaacg ccacgaaccg accgtcgggc gagatcgagc
       61 cgtcgtccga gtcgtcgtcg gcgggggcgc cggccgaggt cttgctgacg aggcgcgtct
      121 tcttcgcctt ccggtcgtgg acgtagacca tgttccccgt gtgcgcggcg ccccccggca
      181 ggttcgaggc ctcggaatcg aaggccacgt agcgaccgtt cgacgagacg gagctctcgc
      241 cgctccagct gtcggcgggc tcgcccgagg aggtcttgct gacgaggcgc gtcttcttcg
      301 tcttccggtc gtggacgtag acgagttcgt aggtgcccgt accccccggg aggttcgtgg
      361 ccccggatgt gaaggccacg aagcgtccgt cgtccgagat cgagccatcg aagctccact
      421 cgttcgccgg ctcgccggcc gagttcttgc tcacgaggcg ggtcttcttc gtctcccggt
      481 cgtggacgta gacgagggcg tgcgtgcccg tggcccccgg caggttcgtg gactcggatg
      541 tgaagaccac ccagcgcccg ttcggcgaga tctcggaccc gtagctccag ctgtccgcgg
      601 gctggcccgc ggaggtctcg ttgacccgcc gggtcttggc aggggccgcc tgcgcgagcg
      661 gcgccacgga gaccagcagc gaaccgacga tcaggcacgc gaagccacgc ataaacggac
      721 gcatggatcc tccctctccg gcgggccctg tcgatgaagc ggggcaaccg gagccagggc
      781 ggggcgcgga gtcaatcggt catccgggcg acggtcagag aacgtccgcg gcctcgcggg
      841 cgaagtaggt gaggatgagg ccggccccgg cgcgacggat cgccgtgagc gtctccagca
      901 tggcggcggt gccgtcgatc cagccccgct cggcggcggc cttgatcatc gcgtactcgc
      961 ccgagacgtt gtaggcggcc accggcacgt ccaccgcgtc gcgtgctcgg cggatcacgt
     1021 cgaggtacgg gagggccggc ttcaccatga ccaggtccgc cccctcggcc acgtcggccc
     1081 ggatctcgcg cagcgcctcg tcggcgttgc ccgggtccat ctggtgggag cggcggtcgc
     1141 cgaaccgggg ggcgccctcg gcggcctcgc ggaagggccc gtagaagctc gaggcgtact
     1201 tggccgcgta ggccaggatc gccgtccccg ggaagcctcc ctcgtccagc gcctcgcgga
     1261 tcgcggccac ctggccgtcc atcatccccg acggcgccac gagatcggct cccgccgcgg
     1321 cctgggccag cgcgatccgc cggtaccgct ccagcgtggc gtcgttgtcc agctcctcgc
     1381 catcgaggac cccgcagtgc ccatggtccg tgtactcgca gaggcagagg tcggcgatca
     1441 gcacgacctc ctcccccagc tcctcgcgga gggcgcggac agcatgctgg ctgatcccct
     1501 cctcggccca cgcctcgccg ccttcggcgt ccttccgctc ggggatcccg aagaggacga
     1561 acgcccggat ccccctctcg gcgagctcgc gggcctcctt ccggagcgac tcgagggtgt
     1621 gctggtggtg gccgggcatc gaggagatgg gggccggctc ggcgatcccc tccttgacga
     1681 agagcggggc gacgaggtgg cgcggggcga ggtcggtctc gcggacgagg tcgcggagcg
     1741 cggtggtcct gcggagccgc ctcggccggt cctgggggaa cgtcatggat cgaatggtag
     1801 agcgtttccg gctaccgcga ggcgaaggcc cgctccaacg cctcgaccag gccctcgagc
     1861 gtgtgaggcg aggccacggc gtccacgcgg aggccggcgg cccgtgcggc gtcggcggtg
     1921 acggggccga tgcaggccac gaggacaccc gccgggacct cggccagggc cgtgaagccc
     1981 tggaccgtgg accgggaggt gaaggtgacg gcgtccacgc ggccctcccc cagggcttgg
     2041 gccaccgcct cgggcagctc gtgcgcgggg atcgtgcggt aggcgtccac ccgcgtcacc
     2101 tgccagccct tgccgcggag cgactcctcg agctcggggg tcgcgatgtc ggcccgggcc
     2161 agcaggacct tctcgctccc ggccgggaac gtctcggcca gggaggccgt ggtgaactcg
     2221 ggggggacga gctcgggctc gatcccgtgg tccccgagcc gctccgcggt gccgctcccg
     2281 acggcggcga ccttggcgtt gagggcgcgg gcgtcgacgc ccagctccgc ggcacgcgcg
     2341 aaccccgcgg cgacgcccgc gcccgacgtg aacaccaccc acgcgtaacc tcgcatcgcc
     2401 gcgtcggtga tcgcctcgtc cagcgggccg ccctccggga ccggttcgat ccggatcgcc
     2461 ggcgccggga gcggaaccgc cccccgctcc cgcagcagcg cggcgagatc ctccacccga
     2521 tccgcgctcc gggtgacgac gaccgtgcgc ccctcgagcg ccgtcacgag gcctcccgga
     2581 ggatcgcgtc cgccccggcc gcccgcagct gctccgcggc gtgcgccgcc acctcgccgg
     2641 ggtcgtcccc ctccacctcg gctcggagga gctcgctccc gtcggggcgg gccaccacgg
     2701 ccagcaggcg caacgtcccg ctgcgggcct cggcgtacgc cccgagcggg agacggcagc
     2761 ccccgcccag cgcgcccacc agcgctcgct ccacctcgaa ggcgcggcgc gttgaggcgt
     2821 ggtccagggt ggccacggcc tctgccaccg ggtcctcggc cctggcctgc accgcgaggg
     2881 ccccctggcc gggcgccggg accatgacgt ccacggggat cggctcggcg tgcgcggggc
     2941 gcacgccgag ccgatccagc cccgccgccg ccagcaccac cgcgtccacc tcgccctggc
     3001 gcagcttccg cagccgcgtg tccacgttcc cccggaacgg caccacgcgc gcagagggat
     3061 acgcccgcag cagctgggcc tgacgccgga ggctcgaggt gcccacggtg ccctcctcgt
     3121 cgaagcccgc cgtcgccgtc accagcacgt cgtaggggag gctgcgcctg ggcacggccg
     3181 ccacgacgac gccctcgggg tcgtccgagg gcaggtcctt cgccgagtgc accgcgaggt
     3241 ccacgccgcc ggccagcagc gcctcggtga tctcggccac gaacagcccc ttcgtgccct
     3301 gaggtgaggc ctccgccggc gcgccccgat cccccgccgt ggtcatcggg acaagctcgg
     3361 cctcgtgccc gcttcccccc aggagctcgg cgacctcgct cgcctgggcg agcgccagcc
     3421 tcgaccgacg cgtgccgatc cggatccgca ctagtccttc ggaggctcca gcccgaacag
     3481 ctcggccagg acccgcgcgt gggcctcgcc cacgcccgcg ccccaccgct ccttcagccc
     3541 gaccacgggg tcgtggagca gcgtcttgac gatgcccttc gtcagggtct caccgcctgc
     3601 cgctcgcggt ccgagaggcc cgacagccgc gaggccagcc gctccagctc ggcggcccgg
     3661 acctcctcac cccgctcgtg gagcgcggcg atcagcgggg ccagcttcgc cgcccggcgc
     3721 tgggtcacga acttcctcgt ctcctcggcg atcacccgtc ccgccgcctc gatctcgtcc
     3781 gcgatcccct cgcgcgaggc ggccacgacg tcgcggaggt cgtcgatgtc ggccacgcgc
     3841 acgccgggga cctcgcgggc cgccgggtcc acatcgcggg gcaccgccag gtcgagcagg
     3901 aacacccgcc gccccccggc ggcccgctcc acgtcctccc gccccaccac gacctgcgtg
     3961 gccgtggtcg acgacacgac gagatcggcc cgggccagcg catccgagag ctcgtccagc
     4021 cccccgtgcg agccccccac ccgcgcggcg agccgctccg ccttctccgg ggtccggttc
     4081 aggaccgtca cctcggcgac cccccgtcgc cggagccacc ccagcgccag cgccgccatc
     4141 tcccccgccc cgaccagcag gacggaccgt ccgcccaggc cccccaggtg ccgcgccgcg
     4201 gcctcgcctc ccgcctccac gaaggcggag ggactggccc cgatcttcgt ctcggccctc
     4261 gcccttcgcc ctgcccggat cgcgtagcgg aacagcgccg agagctgcgt gcccgccgcc
     4321 tcctcacccc gggcccgctg gaaggcctgc cgcacctgcg tcaggatctg gggctccccc
     4381 acgaccatcg agtcgagtcc ggcggccacc gagaacaggt gctcggccgt gtggtcctcg
     4441 tagtgggagt agagaggctc cgcgaactcc tcgggctcca cgcctccgga ctccgacagg
     4501 aaccgcttca ggtcctggaa ccctgtgtga tatccctcca ccgccgcgta gacctcgacc
     4561 cggttgcagg tcgagagcag gacggcctcc tcgaccgacg gcatcgaccg cacgtggtcg
     4621 taggccttgg ggaggtcctc gtcgccccac gccagtcgct cgagcacatc caccgacgcg
     4681 cgacggtacg agacgcccag tgccaggatc ggcatgacgg cctcagtcta cgcggtgggt
     4741 cgctcggcgg tctctccgag cgcctggggc agggcctcgc cccgccgcac ctcgtcgagc
     4801 accgccttcc gctgctcgta gaaggcgagg atctggagct cgaccgagag gtccaccttc
     4861 cggagggaca cgccctcggg gcaggcgatc accacgggcg agaagttgag gatcgagcgg
     4921 atgcccgcct cgaccatccg atccgcaacc tcctgcgccg cagggcccgg cgtgcagatc
     4981 atcccgatct ggatcccgtg ctcgcggacc aggtcgggca tctcctcggg cgggcgaacc
     5041 tcgaccccgg ccacgcgctc gcccacccgg gccgggtcca cgagccccgc cacgcggaag
     5101 ccccgggcgt cgaacccccg gtagttggcc agggcctgcc ccaggttgcc cacgcccgcg
     5161 atcaggacgg gccagtcctg ggtcagcccg agctcgcggc gcacctggtg gatcaggtag
     5221 gcgacgtcgt atcccacccc gcgggtgccg taggagccga ggtaggagag gtccttgcgg
     5281 accttggccg agttgacgcc ggccgcttcg gccagcgcct ccgaggacac cgtggagcgt
     5341 ccgccctcgg ccatctccac cagcacgcgg aggtacacgg ggaggcgccc caccgtggcc
     5401 tcggggatgg ggcgcttcgt catcggtcgc tcgtgcgttc gtgaacgcgc acgacacgat
     5461 ggtaccggcg tgttctcgat cgcacaaggt caggagagca agcggcggag ctcgtccacg
     5521 tccacccggt actcgaaggc ctcctcgccg tccacggcca cgaccgggac cctgagcccg
     5581 tgctcccgct ccagctcggc gtcgccctcg acgtccacct cgctgtagtc gaagcccagc
     5641 tccgcgcgga gcccgaggat cacctcccgg gcctcgtcgc acaggtggca ccccgggcgc
     5701 gcgtacatca ccacctcggc cacggcggca tcctagggcc tcccccgcat ctgcgatact
     5761 cgctccgtgg ccgaggccga cgacgagctc gcccggcgcg ttgccgaccg ggatccggag
     5821 gcgtacgagc agatctaccg gaggtacgcg ccggccgcct acggcgtcgc cctccgggtc
     5881 ctgcgcgacc ccgcactggc ccaggaggtg gcggccgagt cgttcctggc gctctggtcc
     5941 acacccgagg cgtacgatgc ctcgcggggg aggttccggt ccttcttcct ctcgctcgtg
     6001 caccaccgag ccgtggacgc ggtccgccgc gaggagcgcc tacgggagcg tgaacggaag
     6061 gtgaaccggg cgcccctccc ggacgaagac tggagtgagg cggtggtcga ggaggcggac
     6121 ctgatcgacc gccgtcggga ggtccgggag gccctgtccg agctccctcc cgagcagcgc
     6181 gaggcgctgg agctgatgta cttcaggggc tacacgcagt cgaggatcgc ggaggagcga
     6241 gggatcccct tgggaacggt gaagacgagg gtgcacgccg ccatg
//
LOCUS       DASUTW010000301        10649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_359951, whole genome shotgun sequence.
ACCESSION   DASUTW010000301 DASUTW010000000
VERSION     DASUTW010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10649
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359951"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VF058_10325"
     CDS             complement(<1..876)
                     /locus_tag="VF058_10325"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6208736.1"
                     /translation="MLLELHIAGLGVIDDATLDLAPGLNVLTGETGAGKTMVTVGLSL
                     ALGVRARTGMVREGAERARVEARFEASGLAPASEWADDGELVLARSLSADGRSSARAG
                     GQLAQLSTLAELGADLVEVHGQHQTQRLLSPAAQLAFLDRFCGAEHLERLSGYRAAFR
                     RLREVHERLAHLDEAAREREREKDLLEYQVREIESASVRPGERAELEAEERRLGHAER
                     ILELVGAARAALADEDGGADELDAAAASIEEAAALDPAAGDLSQRAAALAVEARDLGG
                     AMRAYAEGLELDPARL"
     gene            1044..1439
                     /locus_tag="VF058_10330"
     CDS             1044..1439
                     /locus_tag="VF058_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEX6208737.1"
                     /translation="MTLNQGFGYVFGAIYALVGLLGFTVTGGVEFAGNQGEQLIGFEL
                     NPLHNIVHIAIGALLIVGAAGGLRASRMTNGAVGAAYLLVGVLGIFMVNEDWDFLALN
                     QADNVLHFATAVIALAIALWADKREPARA"
     gene            complement(1481..2317)
                     /locus_tag="VF058_10335"
     CDS             complement(1481..2317)
                     /locus_tag="VF058_10335"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX6208738.1"
                     /translation="MRAGLVVHPARPRALEVGAEIVTACRERGIATRSLDEVDVGADE
                     RAAGSLADGADLVVSVGGDGTLLRAALLASEAAAPVLGVNVGRLGFLSDIEPPEVPAM
                     LDAFVAGDLVVEERLTVEAEAEGAPWSDRQWALNEIMVEKRARQRVISLAVYVGGEYV
                     TTISGDGVIVATPTGSTAYSFAARGPIVHPRVECLILTPVSPHMVFDRPLVLAPDEQV
                     QIEVRGDEPGLLAPDGRGALELPLGARVRIRRSDRPARLLRRLGAESFFARLARKFSL
                     PS"
     gene            complement(2314..3078)
                     /locus_tag="VF058_10340"
     CDS             complement(2314..3078)
                     /locus_tag="VF058_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017559650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX6208739.1"
                     /translation="MELVRRGLVASRAEAREAIEAGRVLVGGAQAGKASSLVAPEDPV
                     AVEGEGRAYVSRGGVKLAAALDAFGIDPRGRAALDAGAATGGFTDVLLQRGAAHVTAV
                     DVGYGQLAWTIRKDPRVAVLERFNVRDLRPEDLEGRPELVVADLSFISLAKVLPALVR
                     VAADGADLVLLVKPQFEAGRADVGSGGVVRDPAVWARVIREVAAAASEAGAGPLGLVA
                     SPVRGPAGNVEFLLHARVGDQGAELDVEGAVAGVPS"
     gene            complement(3093..3443)
                     /locus_tag="VF058_10345"
     CDS             complement(3093..3443)
                     /locus_tag="VF058_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208740.1"
                     /translation="MRDVVRRYVEAGLGTLKPERAEELVRELVDWSKRNTERVIGIVQ
                     REVKRQLRVAGAATQDELRELRKRVRTLERRLDKAEKPSRSKASSKSTSKSSPSKQAS
                     TRKKSSARRTRPKA"
     gene            3546..3869
                     /locus_tag="VF058_10350"
     CDS             3546..3869
                     /locus_tag="VF058_10350"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX6208741.1"
                     /translation="MERRLEELLRRLRETDGSHVRASLPEPRVLVLEVSDHEASFWTE
                     LGDGRVGELRSGLPDEAHVRIQADSDDIVALADGELAFVPAYLSGRIRVEGGALEMLQ
                     LRRLL"
     gene            3869..4915
                     /locus_tag="VF058_10355"
     CDS             3869..4915
                     /locus_tag="VF058_10355"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6208742.1"
                     /translation="MPGRRFIGRSLRSLGGMLALGRLAARLDDNGPDPAEGDHLGAIR
                     GEVRTVRGPRASSLYTEWFPPAEEGGPTIVFTHGLCLTEAVWHYQKRDLCGGPYGAVT
                     WDLPGHGHSDRIAPGELTQEMGIDALARVVETYAGANGVVLVGHSLGGTLTLGYLARH
                     AEAARERVLGAVIVSTPLVQFAHSVAGRWPGAGLEARIMGKAVRYLVESDLVDRRLAR
                     DVGTDLDTLSYRVVRVGFGKHASPTQVRYVRDVIASVPPQVRADAYRAMTAYDVRSHL
                     GRVEHPILVVIGEEDRLVDPEESRTLATSLPGAEVLGLEDVGHAPFLEAPEAFNDAVL
                     AFARRLSRSEADTA"
     gene            4912..5715
                     /locus_tag="VF058_10360"
     CDS             4912..5715
                     /locus_tag="VF058_10360"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX6208743.1"
                     /translation="MIPIRDANPATRRPYVTWAFIALNVALFLLWQPRGSCPSDLRFT
                     TQAEEEACALELAFTFCHGAIPEELTSFRPMTELPATTQAARSAAQLQELRCPEKGVL
                     GSLFSSMFMHGGLLHLGGNMLYLWIFGNNVEDRMGHLVFPIFYLAGGVVATYAHVATA
                     PSSAIPLVGASGAIAAVLGAYLVMWPHARVTTLVIFFFITVVELPAVVVLGLWFLLQA
                     FQGVGSLGVDVGGVAWWAHIGGFAFGAVVAWLFFRGRREPEVIRPTFEY"
     gene            complement(5712..6908)
                     /locus_tag="VF058_10365"
     CDS             complement(5712..6908)
                     /locus_tag="VF058_10365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX6208744.1"
                     /translation="MDAPADRYDSFLFDLDGVLYRGDRVVPGAPETVAALRRSGGEVV
                     FLTNNSSRTREAVAERLERMGIAADPSQVVTSAMAAASMLKGAGTAFVIGEEGLRAAL
                     SAAGLEVLSGEPESADAVVIGWDRTADYEALRTASVLVQRGARLVATNTDASYPAPGG
                     DLWPGAGALVAAVETTTGVRAEVGGKPQTPLFEEALRIAGMPAERALMIGDRVETDIV
                     GAVRAGIDAVVVFSGAGTPSDLLDHDAPAVAAMNAVPGVLEPRPLVRIRPAGEGGEEA
                     RALADAVGLAAEGGPDEALVAEADGEDVATASVAIEEGTGYLHSVAVRPDARRLHVGT
                     LVVAAGARRAAHAGASELLLVTEDAAPFFARLGFEETDRLGLPGWVAERSRACSQSAT
                     AMRRAI"
     gene            complement(6910..7272)
                     /locus_tag="VF058_10370"
     CDS             complement(6910..7272)
                     /locus_tag="VF058_10370"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6208745.1"
                     /translation="MSEPRDDVYHLLERGRAFLASGHPHQAVMYLERAKLAEPEKASI
                     REPLGRALARIGRWARARREFAKAVAINPADDYAHFALALTCERTGQRRRAIAHLKLA
                     VAMSPREEYRRALGRLDR"
     gene            7302..8483
                     /locus_tag="VF058_10375"
     CDS             7302..8483
                     /locus_tag="VF058_10375"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEX6208746.1"
                     /translation="MPQVAPFDGLVYDPDVVGSVAAATAPPYDVILADELAHFRRGSP
                     YSITHVDLTEEATDAARGDQYTAAGRAFRRWREEGALVRVGRPAVYPYEMSFTYQGAD
                     RRIRGLILEVGLGEWGEGVVPHEKTMRGPVQDRLAVLRATGANLSPIYAVVDGPCPAQ
                     AEILERAAGTEPRFEVEDERGVVHRLWVEDELGDLVSWYRDRRLLVADGHHRYQTALT
                     HRREMREAHGSGPWDAVMMLVVDAATEDPPVLPIHRVVRVDPLPDLPGHLVRDLGEVL
                     AALREDDLHFGAAFTRDGELYHLVGTIEGRTPTVQALHDVLLAGMPGVEEVRFVPDPV
                     VAEEAIRAGTADLALFLPPTRVSDVNRVIASGGRLPQKSTYFWPKPRTGLVIRPLDAD
                     P"
     gene            complement(8487..8801)
                     /locus_tag="VF058_10380"
     CDS             complement(8487..8801)
                     /locus_tag="VF058_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX6208747.1"
                     /translation="MVTVNVVVLAGRLSADPEFVEMPSGDTVARLRLQVPEAGKRVLP
                     LPVAAWTSTTRRSCERLHKGDPVMVRGHLQRRFFRGTGGGRSIMEVVAREVQRLEMPE
                     NA"
     gene            8861..9820
                     /locus_tag="VF058_10385"
     CDS             8861..9820
                     /locus_tag="VF058_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6208748.1"
                     /translation="MTRTFEAAPEIIAIDTRMFGRQDVTSAYLVTGPRPALVETGSAN
                     VAEEVRAGLSSLGVGPDDLAHIVVTHIHLDHAGGAGTLAPHYPSATVWVHERGAPHLA
                     DPSRLMASAARIYGEDGLREMFGAVEPVPQERLRVVSDMEAVDLGEGRTLTALHTPGH
                     ASHHVAILDSSTGGVFVGDALGVFLPDVRILRPATPPPEFDLELAIGSIERIRGVEPS
                     RILFSHFGPVAEVEHVTRLAITRLERWTRIVEEALEETDDPARVAALLREGTARETNP
                     AEVDAARKEGVSQRYEWLSSYEMNASGIMRYLRKKREAAQAGS"
     gene            complement(9817..10422)
                     /locus_tag="VF058_10390"
     CDS             complement(9817..10422)
                     /locus_tag="VF058_10390"
                     /inference="COORDINATES: protein motif:HMM:NF024100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6208749.1"
                     /translation="MEEVRATARRGRVEDVLGLLESAAAAMEKGKATEAQRLAGQAKG
                     LATRSAAVREILGLAYYEGERFREALRELQAYRRISGRADHNHLIADAHRALGTPEKA
                     VPLAREALKARIPAEAKAEAIVVGGAALADLGRYDEAVALLRAFRTNPDEARPFDLRV
                     WYVLGDVLQRAGRTRDAEREFRRILRHDPDAFDVAERLASL"
     gene            complement(10566..>10649)
                     /gene="rrf"
                     /locus_tag="VF058_10395"
     rRNA            complement(10566..>10649)
                     /gene="rrf"
                     /locus_tag="VF058_10395"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1397 a         4118 c         3688 g         1446 t
ORIGIN      
        1 gaggcgtgcg gggtcgagct cgagcccctc ggcgtaggcc ctcatcgcgc cgccgaggtc
       61 gcgcgcctcc accgcgagcg ccgccgcgcg ctgcgagagg tcgcccgcgg ccgggtccag
      121 cgccgcggcc tcctcgatcg aggccgccgc ggcgtccagc tcgtcggcgc ccccgtcctc
      181 gtccgccagc gcggcccgag ccgcccccac cagctcgagg atgcgctcgg cgtgccccag
      241 gcggcgctcc tccgcctcga gctcggcccg ctcgcccggc cggacgctcg cggactcgat
      301 ctctcgcacc tggtactcga gcaggtcctt ctctcgctct cgctcccgcg ccgcctcgtc
      361 gaggtgggcc agccgctcgt ggacctcccg gagacgccgg aaggccgccc ggtagccgct
      421 cagcctctcc aggtgctcag ccccgcagaa ccggtccagg aacgcgagct gagcggcggg
      481 cgaaaggagg cgctgggtct ggtgctgacc gtggacctcg acgaggtcgg cccccagctc
      541 ggccagcgtg gagagctggg ccagctggcc ccctgccctg gcggaggatc ggccgtcggc
      601 cgagagcgac cgggccagca ccagctcgcc gtcgtccgcc cactcggagg ccggagccag
      661 gcccgaagcc tcgaaccgcg cctccacccg ggcccgctcg gccccctccc ggaccatccc
      721 ggtccgggcc cgcacgccga gggcgagcga gagccccacc gtgaccatcg tcttgccggc
      781 gcccgtctcc cccgtgagga cgttgagccc cggggcgagg tcgagggtgg cgtcgtcgat
      841 caccccgagc ccggcgatgt ggagctccag gagcatgtca gcggcgatcg tacctccacc
      901 caccgacgcg atcgcggatg tcggtccgga tggtggcctc gcccccctcc ccggcatacg
      961 atgacgctcg acgggccgca gagcggctat ccgggccggg cccgtcatcc gagctccgga
     1021 gaccggaagg agggaacggg gacatgaccc tcaaccaggg cttcggctac gtgttcggag
     1081 ccatctacgc cctcgtgggc ctgctcggtt tcaccgtcac cggcggggtc gagttcgcgg
     1141 gcaaccaggg cgagcagctg atcggcttcg agctcaaccc gctccacaac atcgtgcaca
     1201 tcgcgatcgg cgccctgctg atcgtcgggg ccgccggggg cctgagggcc tcccgcatga
     1261 ccaacggcgc ggtgggcgcg gcgtacctgc tggtgggggt cctcggcatc ttcatggtga
     1321 acgaggactg ggacttcctg gcgctgaacc aggccgacaa cgtccttcac ttcgccacgg
     1381 cggtgatcgc cctcgcgatc gcgctgtggg cggacaagag ggagcccgcc cgagcctgac
     1441 cccggaggcc gggcccgtcc cctccgggac gggcccggcc tcacgacggc agcgagaact
     1501 tcctggccag ccgggcgaag aacgactccg cccccagtcg gcgcaggagg cgagccggcc
     1561 ggtccgagcg ccggatcctc acgcgggccc ccagcggcag ctccagcgcc cccctcccgt
     1621 cgggagcgag gagcccgggc tcgtctcccc tcacctcgat ctggacctgc tcgtcggggg
     1681 cgaggacgag cggtcggtcg aacaccatgt gcggagagac cggggtgagg atcaggcact
     1741 cgaccctcgg atggacgatc ggtcctcggg ccgcgaacga gtaggccgtc gagcccgtgg
     1801 gcgtggccac gatcaccccg tcgcccgaga tcgtggtgac gtactcgccc ccgacgtaca
     1861 cggccagcga gatcacgcgc tggcgggccc gcttctccac catgatctcg ttgagcgccc
     1921 actgacgatc gctccacggc gcgccctcgg cctcggcctc cacggtgagc cgctcctcga
     1981 cgacgagatc cccggccacg aaggcgtcca gcatcgcggg gacctccgga ggctcgatgt
     2041 cgctgaggaa ccccaacctg cccacgttca ccccgagcac gggcgccgcc gcctccgagg
     2101 ccagcagcgc cgcccgcagc agcgtcccgt ccccgcccac agagacgacg aggtcagcgc
     2161 cgtccgcgag cgatccggcc gcccgctcgt ccgccccgac gtccacctcg tccagggacc
     2221 tggtggcgat ccctcgctcc cggcacgcgg tcacgatctc ggcccccacc tcgagcgccc
     2281 gggggcgggc cggatggacc accagccccg ccctcatgac ggcacccccg cgaccgcccc
     2341 ctccacgtcg agctcggcgc cctggtcgcc caccctggcg tggagcagga actcgacgtt
     2401 cccggcgggc ccccggaccg gcgaggcgac gagccccaac ggtccggccc ccgcctcgct
     2461 cgccgccgcg gccacctccc ggatcacccg ggcccacacg gccggatcgc gcacgacgcc
     2521 gccgctcccc acgtcggccc gacccgcctc gaactgcggc ttcacgagca gcacgaggtc
     2581 ggcgccgtcc gcggccacgc ggaccagcgc gggcagcacc ttcgccagcg agatgaagga
     2641 gaggtccgcg accacgagct cgggccgccc ctccaggtcc tccggccgca ggtcgcgaac
     2701 gttgaagcgc tcgaggaccg ccacccgggg atccttccgg atcgtccacg cgagctggcc
     2761 gtagccgacg tcgaccgcgg tcacgtgcgc cgctccccgc tgcaggagga cgtcggtgaa
     2821 gccgccggtg gcggccccgg catcgagggc ggctcggccg cgggggtcga tcccgaacgc
     2881 gtccagcgcc gcggcgagct tcaccccgcc ccgggagacg taggcccggc cctcgccctc
     2941 cacggccacg gggtcctccg gggccacgag cgacgaagcc ttgccggcct gggcgccgcc
     3001 gaccagaacg cggcccgcct cgatcgcctc ccgggcctcg gcgcgcgagg caacgaggcc
     3061 ccgccggacc agctccacgt cgagccgccg cctcaagcct tcgggcgggt gcgcctcgcc
     3121 gaggacttct tcctcgtcga ggcctgcttc gagggcgacg acttcgaggt cgacttcgag
     3181 gaggccttcg acctcgaggg cttctcggcc ttgtccagcc ggcgctccag cgtgcggacc
     3241 cgcttccgca gctcccgcag ctcgtcctgg gtggcggccc ccgccacccg cagctgtcgc
     3301 ttcacctcgc gctgcacgat cccgatcacg cgctcggtgt tcctcttcga ccagtccacg
     3361 agctcccgca ccagctcctc tgcccgctcg ggcttcagcg tgcccagccc cgcttccacg
     3421 tacctacgca cgacgtctcg catgagggaa gcataggaca gagcagcgag cgtggtgccg
     3481 gtatactcgg gccgttctcc ctttccatcc ccgaagcagg agcgtcgttg gcgagcctag
     3541 gcgaggtgga gcgaaggctg gaggagctgc tgaggcggct ccgagagacg gacgggtcgc
     3601 acgtccgcgc gtccctcccg gagccccgcg tgctcgtcct cgaggtgagc gaccacgagg
     3661 cgagcttctg gaccgagctg ggcgacggtc gggtgggcga gctccgctcc ggcctccccg
     3721 acgaggcgca cgtccggatc caggccgact ccgacgacat cgtggccctc gcggacggcg
     3781 agctcgcgtt cgtccccgcc tacctctcgg gccggatacg ggtcgagggc ggcgccctcg
     3841 agatgcttca gctccgaagg ctgctctgat gcccggacgc cgcttcatcg gcagatcgct
     3901 ccgctcgctc ggagggatgc tggccctggg ccggctggcc gcccgcctcg acgacaacgg
     3961 acccgacccc gccgagggag accatctcgg ggcgatccgg ggggaggtcc ggacggtccg
     4021 gggccctcgg gcctcctcgc tctacaccga gtggttccct cccgcggagg agggaggccc
     4081 cacgatcgtc ttcacccacg gcctctgtct gaccgaggcc gtgtggcact accagaagcg
     4141 ggacctgtgc ggcggaccct acggcgcggt cacgtgggac ctccccggcc acgggcactc
     4201 cgacaggatc gcccccgggg agctgacgca ggagatgggg atcgacgcgc tggcccgcgt
     4261 cgtggagacg tacgcggggg cgaacggggt cgtcctcgtg ggccacagcc tgggcgggac
     4321 cctcaccctc ggatacctgg cgcggcacgc ggaggcggct cgggagcgcg ttctcggcgc
     4381 ggtgatcgtc tccaccccgc tggtgcagtt cgcccactcg gtggccggcc ggtggccggg
     4441 agcgggactc gaggccagga tcatggggaa ggcggtgcgc tacctggtcg agagcgacct
     4501 cgtggaccgg aggctggcca gggacgtcgg gaccgacctc gacaccctct cctaccgggt
     4561 cgtgcgcgtg ggcttcggga agcacgcctc gcccacgcag gtgcgctacg tccgcgacgt
     4621 gatcgcctcg gtcccgcccc aggtgcgggc cgacgcgtac cgggccatga ccgcctacga
     4681 cgtccggtcc cacctgggcc gggtggagca ccccatcctc gtcgtgatcg gcgaggagga
     4741 ccgcctcgtg gatcccgagg agtcgcggac gttggcgacc tcccttcccg gagccgaggt
     4801 gctgggcctc gaggacgtgg gccacgcccc cttcctggaa gcccccgagg cgttcaacga
     4861 cgcggtgctg gcgttcgccc gccggctgag ccggtccgag gcggacacgg cgtgatcccg
     4921 atccgcgacg cgaacccggc cacccgccgc ccctacgtta cgtgggcgtt catcgcgctg
     4981 aacgtggcgc tattcctgct ctggcagccc cggggctcct gccccagcga cctccgattc
     5041 accactcagg ccgaggagga ggcctgcgcc ctcgagctcg ccttcacctt ctgccacggg
     5101 gccatccccg aggagctgac ctcgttccgc ccgatgaccg agctcccggc caccacgcag
     5161 gccgcgcggt ccgccgccca gctccaggag ctgcgctgcc ccgagaaggg ggtgctgggc
     5221 tcgctgttca gctcgatgtt catgcacgga gggctgctcc atctgggcgg gaacatgctc
     5281 tacctctgga tcttcgggaa caacgtcgag gaccgcatgg gccacctcgt gttccccatc
     5341 ttctacctgg cgggaggggt ggtggccacc tacgcgcacg tggccacggc gccctcgtcc
     5401 gccatcccgc tggtcggcgc ctccggcgcg atcgccgcgg tgctcggcgc ctacctcgtg
     5461 atgtggcccc acgcccgcgt gaccacactc gtcatcttct tcttcatcac agtggtcgag
     5521 ctgccggcgg tggtggtgct cggcctctgg ttcctgctgc aggccttcca gggcgtgggg
     5581 agcctgggcg tggacgtggg cggcgtggcc tggtgggccc acatcggggg gttcgccttc
     5641 ggggccgtcg tggcgtggct gttcttccgg gggagacgcg agccggaggt gatccgcccg
     5701 acgttcgagt actagatcgc ccggcgcatc gcggtcgcgc tctgcgagca ggcccgggac
     5761 cgctcggcca cccagcccgg gaggcccagg cggtcggtct cctcgaagcc cagccgggcg
     5821 aagaacgggg ccgcgtcctc ggtgacgagg agcagctcgc tcgcgcccgc gtgggcggcc
     5881 cgacgcgcgc cggcggccac caccagcgtg ccgacgtgga ggcggcgcgc gtcgggccgc
     5941 accgccaccg aatgcaggta ccccgtcccc tcctcgatcg ccaccgacgc ggtggccacg
     6001 tcctccccgt cggcctcggc cacgagcgcc tcgtcagggc ctccctccgc cgccagcccg
     6061 accgcatcgg ccagagcccg ggcctcctca cctccctccc cggccgggcg gatccgcacc
     6121 aggggcctgg gctccaggac ccccggaacg gcgttcatgg cggccaccgc cggggcgtcg
     6181 tgatccagca ggtccgatgg ggttccggcg cccgagaaga cgaccacagc gtcgatcccc
     6241 gcccggaccg ccccgacgat gtcggtctcg acgcggtcac cgatcatcag cgcgcgctcg
     6301 gcgggcatcc ccgcgatgcg gagggcctcc tcgaacaggg gcgtctgggg cttccccccg
     6361 acctcggccc ggacgcccgt ggtcgtctcc acggcggcca ccagcgcccc cgcccccggc
     6421 cacagatccc ctcccggagc cgggtacgag gcgtccgtgt tggtggccac gagcctcgct
     6481 ccccgctgga ccagtaccga cgcggtgcgg agggcctcgt agtcggccgt gcggtcccag
     6541 ccgatcacca cggcgtcggc gctctccggc tcgccggaga ggacctccag ccccgccgcg
     6601 gagagcgccg cgcgcagccc ctcctcgccg atcacgaacg cggtgccggc ccccttgagc
     6661 atcgaggccg cggccatggc cgacgtcacg acctgcgagg ggtccgcggc gatccccatc
     6721 cgctccagcc gctccgcgac cgcctcgcgg gtgcgagagg agttgttcgt caggaagacc
     6781 acctctcccc ctgaccgccg cagggcggcc acggtctcgg gagccccggg cacgacccgg
     6841 tcgccccggt agaggacccc gtccaggtcg aagaggaacg agtcgtagcg gtcggcgggg
     6901 gcgtccacgt cagcggtcga ggcggcccaa cgcccgccga tactcctccc ggggcgacat
     6961 cgccaccgcg agcttcaggt gcgcgatggc ccgcctgcgc tgccccgtgc gctcgcaggt
     7021 gagggccagc gcgaagtggg cgtagtcgtc cgcggggttg atcgccacgg ccttggcgaa
     7081 ctcgcgccgc gcccgcgccc agcgcccgat cctggccagg gccctcccca ggggctcgcg
     7141 gatcgaggcc ttctcgggct cggcgagctt ggcccgctcc agatacatca cggcctggtg
     7201 gggatgtccc gaggcgagga aggctcgccc ccgctccagg aggtggtaga cgtcgtctct
     7261 cggctcggac atcccgctcc tcgatggtct atacagacgg cgtgccacag gtcgccccct
     7321 tcgatgggct cgtctacgac cccgacgtgg tggggagcgt cgccgccgcg accgccccac
     7381 cgtacgacgt catcctcgcc gacgagctgg cccacttcag gcggggcagc ccctacagca
     7441 tcacccacgt ggacctgacc gaggaggcca cggatgccgc ccgcggcgat cagtatacgg
     7501 ccgccggccg ggccttccgg cggtggcggg aggagggcgc gctcgttcgg gtgggacggc
     7561 ccgccgtgta cccctacgag atgtccttca cgtaccaggg ggccgaccgg cgcatccggg
     7621 ggctgatcct cgaggtgggc ctcggggagt ggggcgaggg cgtggtccct cacgagaaga
     7681 cgatgcgggg gcctgtgcag gaccgcctgg ctgtcctccg agctacgggc gcgaacctct
     7741 cgcccatcta cgcggtggtg gacggcccct gcccggccca ggccgagatc ctggagcgcg
     7801 ccgccgggac ggagccccgc ttcgaggtcg aggacgagcg gggcgtcgtc caccgcctgt
     7861 gggtggagga cgagctcggc gatctcgtct cctggtaccg ggatcgccgc ctgctcgtcg
     7921 ccgacggcca ccatcgctac cagacggcgc tcacgcaccg ccgggagatg cgtgaggccc
     7981 acggatcagg gccctgggac gccgtgatga tgctggtcgt ggacgcggcc accgaggacc
     8041 cgccggtcct gcccatccat cgcgtggtcc gcgtggaccc gctccccgac ctccccggcc
     8101 acctcgtgcg cgatctcggc gaggtcctgg ccgcccttcg agaggacgac ctccacttcg
     8161 gagcggcctt cacccgagac ggcgagctct atcatctcgt gggcacgatc gagggccgga
     8221 ccccgaccgt ccaggcgctc cacgacgtcc tgctggccgg gatgccgggc gtggaggagg
     8281 tccgcttcgt ccccgacccc gtcgtggccg aggaggcgat ccgcgcgggg accgccgatc
     8341 tcgcgctttt cctccctccc acgagggtga gcgacgtgaa ccgggtgatc gcctccggcg
     8401 gccgcctgcc ccagaagtcg acctacttct ggcccaagcc caggaccgga ctcgtcatcc
     8461 ggccgctcga cgccgacccc tgaggctcaa gcgttctccg gcatctccag ccgctgcacc
     8521 tccctggcca ccacctccat gatcgatcgc ccgcccccgg tcccacggaa gaacctccgc
     8581 tggagatgac cacggaccat cacgggatcg cccttgtgca gacgctcgca gctccggcgc
     8641 gtggtgctcg tccaggccgc gaccgggagg gggaggacgc gcttgccggc ctcggggacc
     8701 tggaggcgga gcctggccac cgtgtcgccc gacggcatct cgacgaactc ggggtccgcc
     8761 gagagccgcc cggcgagcac gaccacgttc accgtgacca tgcccacctc ctttcggccg
     8821 gtaccgtagc aggggggtcc gacacgatag actccggcgc gtgacccgca cgttcgaggc
     8881 cgcccccgag atcatcgcca tcgacacgag gatgttcggt cgccaggacg tgacgtcggc
     8941 ctacctcgtc accggccccc gaccggctct cgtcgagacg ggctcggcca acgtggccga
     9001 ggaggtccgg gcggggctca gctccctcgg ggtcgggccg gacgacctgg cccacatcgt
     9061 ggtcacgcac atccacctcg accacgcggg cggcgccggc acgctcgccc cccactaccc
     9121 gtcggccacg gtctgggtcc acgagcgggg cgcgccgcac ctggcggatc cctcccggct
     9181 catggccagc gccgcgcgca tctacggaga ggacggcctg cgcgagatgt tcggggcggt
     9241 ggagccggtt cctcaggagc ggcttcgggt cgtgagcgac atggaggccg tggacctcgg
     9301 cgaggggcgc accctgaccg cgctccacac cccaggccac gccagccacc atgtcgcgat
     9361 cctcgactcc tccacgggag gggtcttcgt gggggacgcg ctcggggtct tcctcccgga
     9421 cgtgcggatc ctgcggccgg ccaccccgcc ccccgagttc gacctggagc tcgcgatcgg
     9481 cagcatcgag cggatccggg gcgtggagcc ctcgcggatc ctgttcagtc acttcgggcc
     9541 ggtggccgag gtcgagcacg tcacccggct cgcgatcacg cgcctggagc gatggacccg
     9601 gatcgtggag gaggcgctcg aggagaccga cgaccccgct cgcgtcgcgg ccctgctccg
     9661 cgaggggacg gcgcgggaga cgaaccccgc ggaggtcgac gcggcccgga aggagggagt
     9721 ctcccagcgc tacgagtggc tctccagcta cgagatgaac gcgtcgggga tcatgcgcta
     9781 cctccggaag aagcgcgagg ccgcgcaggc cggttcctag agcgaggcga gtctttccgc
     9841 gacgtcgaag gcgtccgggt cgtggcggag gatcctccgg aactcccgct ccgcatcccg
     9901 ggtccgtccg gcccgctgga ggacgtcccc caggacgtac cagacgcgca ggtcgaacgg
     9961 gcgcgcctcg tctgggttcg tgcggaaggc gcggagcagg gccacggcct cgtcgtagcg
    10021 accgaggtcg gcgagcgcgg cgccccccac gacgatcgcc tcggccttgg cctccgcggg
    10081 gatcctcgcc ttcagggcct ccctggccag gggcaccgcc ttctccggcg tgcccagcgc
    10141 ccgatgggcg tcggcgatca ggtggttgtg atcggcccgc ccgctgatcc ggcggtacgc
    10201 ctggagctcg cgcagcgcct cccggaaccg ctcgccctcg tagtacgcga gccccaggat
    10261 ctcgcggacg gcggcggatc tcgtggccag gcccttggcc tgcccggcga gccgctgggc
    10321 ctcggtcgcc ttccccttct ccatcgcggc cgccgccgac tctaggagcc ccaagacgtc
    10381 ctcgacccgc ccccgccggg ccgtggcccg gacttcctcc acgacctcct tcgggagggt
    10441 ggaccgccgc cgggtgggtc ggggtccgga ccggcgcggc ggcccgcccc ctcgacgccc
    10501 tcgtcctcgt ctccgatccc ccatgggcag agcgtacccg agccgccggg cctcctcgaa
    10561 agatgtctcg gcggcgacct actctcccac cccgtctcca gggcagtacc atcggcgctg
    10621 gagggcttaa cttccgggtt cgggatggg
//
LOCUS       DASUTW010000302         2687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_879647, whole genome shotgun sequence.
ACCESSION   DASUTW010000302 DASUTW010000000
VERSION     DASUTW010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879647"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..877)
                     /locus_tag="VF058_10400"
     CDS             complement(50..877)
                     /locus_tag="VF058_10400"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014911935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX6208750.1"
                     /translation="MCLAGTAETVRKHRHEGPVLTRRRFLAAGGAAAALAAMPAPALA
                     RSTRHRHTQDLTHVFRDGFPVYTGNSPSRQTLVTIPNDGFYAQRWTFDEHTGTHLDAP
                     GHFIEGNRLADELPPDELVRPLAVVDISGRAAKDPDAEVRVKDLVAFERRHGRIPRRA
                     VVAMHSGWDARAGDRDSYLNPDGSGVFHFPGFSLEAVEWLLERRDVQGIGVDTSSLDF
                     GPSTTFIVHTVLLGEERYGLEGLANLGRVPANRATVFVGLIPWEEGSGGPCRVIARW"
     gene            1019..1201
                     /locus_tag="VF058_10405"
     CDS             1019..1201
                     /locus_tag="VF058_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX6208751.1"
                     /translation="MQKQFEGRWDQVKGKVKETWGDLTDDELDQTEGKWEQLVGKIKA
                     KTGESQEEIERKLGSM"
     gene            complement(1266..1856)
                     /gene="cysC"
                     /locus_tag="VF058_10410"
     CDS             complement(1266..1856)
                     /gene="cysC"
                     /locus_tag="VF058_10410"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX6208752.1"
                     /translation="MTEIYAKEGMRTSNAPKPGNKGFTLWMTGLSGSGKSTLAERLVP
                     IFDERGLSVELLDGDVIRTHLSKGLGFSKEDRDTNILRIGWVANRLSAHGAAVITSAI
                     SPYKAVRDEVRAGMPERFVEVFVSASVEECARRDTKGLYERAFKGEIANFTGVSDPYE
                     APESPEILVDTERFSIEECLDQIVARLEELGHLPAS"
     gene            complement(1853..>2687)
                     /locus_tag="VF058_10415"
     CDS             complement(1853..>2687)
                     /locus_tag="VF058_10415"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6208753.1"
                     /translation="HMVRTKPWDFFMMVEMGTDRLHHVFWQYCDPDHPKYEPGNPYES
                     AFVDYYRMLDGEVGALLETFPDDVAVILMSDHGARAMAGGVLFNDWLMQEGYLVMERP
                     LTQPTPIAKAPIDWSKTTAWGDGGYYGRLFLNVKGREPQGTIEQEDYERVRAEIAGKL
                     EEMKDPDGRPLGTRVFRPENVYPEVRGVAPDLIVYFGDLEWRSVGTVGTGETFTYEND
                     TGPDGANHDKRGVFALSNLPGVEPGMVEAGFRLIDVGPTLMSLYDVPIPEGVEGRSIL
                     S"
BASE COUNT          423 a          931 c          932 g          401 t
ORIGIN      
        1 gcgggtctga catgaaccgc tcgtcagacc cgcccgaaac gcggcgatcc taccagcgcg
       61 cgatcacccg gcaggggccg ccggagccct cctcccacgg gatcaggccg acgaagaccg
      121 tggcgcggtt cgccgggacc cgccccaggt tggcgaggcc ctccagcccg tagcgctcct
      181 cccccagcag caccgtgtgc acgatgaagg tcgtggaggg gccgaagtcg agggaggacg
      241 tgtccacccc gatcccctgg acgtcgcgcc gctcgagcag ccactccacg gcctcgagtg
      301 agaacccggg gaagtggaac acgccggacc catccggatt gaggtacgag tcgcgatccc
      361 cggcccgcgc gtcccacccc gagtgcatgg ccacgaccgc gcgcctgggg atcctcccgt
      421 ggcgccgctc gaaggccacg aggtccttca ccctcacctc cgcatcgggg tccttcgccg
      481 cgcggcccga gatgtcgacc acggcgaggg gacggacgag ctcgtccggc ggcagctcgt
      541 cggccagccg gttcccctcg atgaagtggc cgggggcgtc gagatgcgtg cccgtgtgct
      601 cgtcgaacgt ccaccgctgc gcgtagaagc cgtcgttcgg gatcgtcacg agcgtctgcc
      661 ggctcgggga gttcccggtg taaaccggga acccgtcgcg gaagacgtgg gtgagatcct
      721 gcgtgtggcg gtgacgggtg ctccgtgcga gcgccggggc gggcatcgcg gcgagcgccg
      781 ccgccgctcc ccccgcggcg aggaacctcc gccgggtaag gaccggcccc tcgtgccggt
      841 gcttccgaac cgtctcagcg gtaccagcca ggcacatggc tccacctcct gcagctcggt
      901 aggtgaggag cctacgcccg cgtcgagggc ggacacaagc cggcgctcgc tccggttttc
      961 acgcccggac ccccgggtat ccaaggggga cgaacggaag gagcggacca tgggtggcat
     1021 gcagaagcag ttcgagggcc ggtgggacca ggtcaagggc aaggtcaagg agacctgggg
     1081 cgacctcacc gacgacgagc tcgaccagac cgaggggaag tgggaacagc tcgtcggcaa
     1141 gatcaaggcc aagacgggcg agtcgcagga ggagatcgag cgcaagctcg gaagcatgta
     1201 agacgccccc acgaggggca acgagggcgg caccctacga gcgggcgggg gtgccgctct
     1261 ctacgtcagg atgcaggcag gtggcccagc tcctccagac gagccacgat ctggtccagg
     1321 cactcctcga tcgagaagcg ctcggtgtcc acgaggatct cgggcgattc cggcgcctcg
     1381 taggggtcgc tcaccccggt gaagttggcg atctcgccct tgaaggcccg ctcgtagagc
     1441 cccttcgtgt cgcggcgggc gcactcctcg acggaggccg agacgaacac ctcgacgaag
     1501 cgctccggca tccccgcgcg gacctcgtcg cggacggcct tgtaggggct gatcgcgctc
     1561 gtgatcaccg cggcgccgtg ggcggagagg cggttcgcca cccacccgat ccggaggatg
     1621 ttcgtgtcgc ggtcctcctt ggagaacccg aggcccttgg agaggtgcgt gcggatcaca
     1681 tcgccgtcca gcagctccac cgagagcccg cgctcgtcga agatcggcac caggcgctcg
     1741 gcgagggtgg acttccccga gccggagagg ccggtcatcc agagggtgaa gcccttgttg
     1801 cccggcttcg gggcgttgga ggtccgcatg ccctccttcg cgtagatctc ggtcacgaga
     1861 ggatgctcct tccttccacg ccctcgggga tcgggacgtc gtacaggctc atcagggtgg
     1921 gacccacgtc gatcaggcgg aaccccgcct ccaccatccc gggctcgacg cccgggaggt
     1981 tggagagcgc gaacaccccc cgcttgtcgt ggttcgcgcc atcgggtccc gtgtcgttct
     2041 cgtaggtgaa ggtctcgccg gtgccgaccg tccccaccga ccgccactcc aggtccccga
     2101 agtacacgat gaggtcgggc gccaccccgc gcacctccgg gtagacgttc tccggacgga
     2161 acacccgcgt gccgagcggc cggccgtccg ggtccttcat ctcctcgagc ttcccggcga
     2221 tctcggcccg gacccgctcg tagtcctcct gctcgatcgt gccctggggc tcccgtccct
     2281 tcacgttgag gaagaggcgg ccgtagtagc cgccgtcgcc ccacgcggtg gtcttggacc
     2341 agtcgatcgg ggccttggcg atcggcgtgg gctgggtgag aggccgctcc atgacgaggt
     2401 agccctcctg catcagccag tcgttgaaga ggacgcctcc ggccatggcc cgagcgccgt
     2461 ggtcgctcat gaggatcacg gcgacgtcgt cggggaaggt ctccagcagc gcgccgacct
     2521 cgccgtcgag catccggtag tagtccacga acgcgctctc gtaggggttg cccggctcgt
     2581 acttcgggtg gtccggatcg cagtactgcc agaagacgtg gtggagccgg tcggtcccca
     2641 tctcgaccat catgaagaag tcccagggct tggtcctcac catgtgg
//
LOCUS       DASUTW010000303        15108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_361067, whole genome shotgun sequence.
ACCESSION   DASUTW010000303 DASUTW010000000
VERSION     DASUTW010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15108
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361067"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..877)
                     /locus_tag="VF058_10420"
     CDS             complement(29..877)
                     /locus_tag="VF058_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6208754.1"
                     /translation="MRKQRYLWLIGALTALMLVASACGGDEDTDAEPTAEPTETEETA
                     PAIETLQEGVLTVGSDIPYPPFEFREGGELTGLDIDLITEMATRLDLEVEVIDTDFDT
                     IFTQLAGGRFDVVASATTITPEREEEVNFTEGYYNAQQSLTVLADSGITSVDELGEEH
                     SVAVQRGTTGEAWAEENLAPQGVTLRTFTQTPDLYSAVEGGAVTGAINDEPSAIAEIA
                     ARDGLELVQTIDTGEVYGIAVNPGNEPLLEELNRVLAEMIEDGTYEEIYGRYPDLPPG
                     GNVAAG"
     gene            994..2160
                     /locus_tag="VF058_10425"
     CDS             994..2160
                     /locus_tag="VF058_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX6208755.1"
                     /translation="MSSSLAPRADFPVLEEAAYLNAASIALMPLPVRRSMQAFMEEIA
                     GRGTIGFDDDAEAAVYDGVRSRAARLLGSEPADVAVTTSASEALGQVAWWLRPGEGEN
                     VVSIDIEFPSVTYAWMRVAEETGLEVRLVRALEDPLRLTLDRVAELVDDRTAAISVSH
                     VQYATGHLLDLRALADLAHAHGALAVVDATQSAGVVPIDVQEADVDVLVSGGYKWLCA
                     TFGAAICYLHPRIAERFLPPLISWRSTPNPPDFDATRMPLAEGARRLEYSTVAYPSGI
                     GLGEAIEYILGLGVDRILEHDLGLTDKVAAGLEELGGTLISAPREVPRGSIATARFPG
                     RRPERLVERLIQAGVIAGPRLGGVRISPHLYNDVSDVDRALADLERILETPEDD"
     gene            2180..3340
                     /locus_tag="VF058_10430"
     CDS             2180..3340
                     /locus_tag="VF058_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX6208756.1"
                     /translation="MSGSLVPQDHFPVPDGTTYLNSASISLTARPVREEMRRFEDEVV
                     HLGTINLDDEAENRVYEGVRGSAAALLGCDADDIAVMTSATEALCQVAWWLRPGEGEN
                     VVSIDIEFPSVTYPWLRLAEDEGLEVRLVRAMEDPLRLTLDRVAELVDERTAAICVSH
                     VQYATGHLLDPTALSELAHAHDALLILDTTQSSGAVPLDVRSTDQDVVIASAYKWLTG
                     VPGAAFAYLRPEVAERFRPPFVGWRTTGEGAMAFDATRISLAEGGRRMEYATVAYEAG
                     ISLGAATRYLLDVGIEKIHEHDRGLCALLAEGLRVRGAEPLTPEDPDGRGTIVAARFP
                     GRDPFELAGALARRSVFVAPRLGGLRFSPHLHNDEDDVGRALEALDAALEGA"
     gene            3340..4320
                     /locus_tag="VF058_10435"
     CDS             3340..4320
                     /locus_tag="VF058_10435"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="cyclodeaminase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6208757.1"
                     /translation="MLLLSEEEIRRSVSSKEALEAVAEAFAALARGEARLPGVIYLDL
                     QEFNGDVHVKGAHMRGASHFAIKVAGGWYDNPSRGLPVGTGLVLVFSAETGAPAALLL
                     DNGYLTDLRTGAAGALAARHLARDPLSKVAMIGAGLEARFQLRALADVRALPRIEAWS
                     RDPANARRFAEEMRDELGADVEVAPDVESAVREADLVVTATPAREPLVRADWLSSGVH
                     VTALGSDGPAKQELDVDVLARADLVVVDDLEQAAEKGELHHALEAGALTRDRVAAQLG
                     DLVSGDHPGRTDDSQITVADLTGVGVQDAAVASLALERAEAAGLGRDLPT"
     gene            4322..5587
                     /locus_tag="VF058_10440"
     CDS             4322..5587
                     /locus_tag="VF058_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HEX6208758.1"
                     /translation="MDVRLDDRDRELLDGAHGEAGSLAMRILVRMARAQGAAGLRDVT
                     RAHIDGCLYHGRAGLDFARRMVDGGGRVAVPTTLNVASLDLLHPERYRGDPETARHAR
                     SLMDAYVELGCRPTWTCAPYQLPEARPALGEHVAWAESNAIVFANSVLGARTDRYGDF
                     LDICAAITGRVPEAGLHLDEHRRGEIMFRVEGIGSGLLGDPVLYPLVGHVVGRASGSR
                     VPVLVGLPGDASEDDLKALGAAAASSGSVALFHAVGLTPEAPTLDEALGGRPPLREET
                     IGPERLRAAREELTTSRGERLTGVSLGTPHFSVEEFRGLLEALEGGRIHPDVECYVST
                     GRDVLAEVESRGWLAPIEAAGVRIVTDTCTYVTPIMPPGGVVMTNSGKWAYYAPGNLG
                     IATVFGSLEECVRSALAGRVTVDEELWRG"
     gene            5580..5999
                     /locus_tag="VF058_10445"
     CDS             5580..5999
                     /locus_tag="VF058_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HEX6208759.1"
                     /translation="MAEGRALASGMAEGVLLVLREPLSFWGGMDPVTGEVVDAHHPQR
                     GEVLSGRVVAMPSGRGSSSASSVLAEAIRTGTAPAALVLGEPDPIVALGSVVASELYG
                     SACPVVVLDDAAYDLLRTGDRLRVTADEHGARVDMLA"
     gene            complement(5996..7420)
                     /locus_tag="VF058_10450"
     CDS             complement(5996..7420)
                     /locus_tag="VF058_10450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX6208760.1"
                     /translation="MTGRAKLIAGVAAGTVAVFVFLVRGGPSQEPELGRRSLSGPTAS
                     PSPSPEPPGPALRVRVGRVSAVTIHGRPRRPRLRAARRGVERTIEELYTRGFLEPVAG
                     GASPRVDDLFVPQTRKQARRDREVLTLGKASSRITTLRAPRARLNLRVLTDRGRRPTA
                     VLARARFAARAFRAGIEDEIRHRGSYLLRRAAGRWRIVSYDVRGRIPSAGKVARPMGA
                     PATRRGVLFILAIGSDARPGERVAGARADSLHIIGVNLRKGSAGIVGIPRDSFVPIPG
                     VGTRKINEALFYGGPELTVRTVERLSGIRIGAYLLTGFQGFRAAVTGVGGVRTVIPYA
                     MSDAASEAFFRPGRRRLGGRGALAFSRNRHDAPGGDFGRSLNQGRLLMATLREFRSDL
                     RRDPAALVRWVAVGARNLQTDLSLQDTLGLLLATTSVDPGKVRNTVVSGSGAMVGGQS
                     IVRLGSGAQATFRDLRSDGLLNGR"
     gene            complement(7417..8448)
                     /gene="solA"
                     /locus_tag="VF058_10455"
     CDS             complement(7417..8448)
                     /gene="solA"
                     /locus_tag="VF058_10455"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HEX6208761.1"
                     /translation="MDADVVVVGAGAVGSAAARELARRGLDVIALERFRIGHDRGSSH
                     GDARIFRHSYDAPDWVRMMKEAMGLWAELEDEAGEELLTRTGGIDLDAPPENATSLDA
                     EGMGYEVLSGAAVMERFPSLSLAPEEEVLFQADAGIVHADRAWRALVEGARRAGAEVR
                     EGVQVVELRADEDGVEARTDGGTISARVGVVAAGSWALDLLRPVGIAVPARVTRESPA
                     YFESEPPPVVVVDWHGEHLTYALPSPGFGLKAAEHHAGPEVDPDDWGGPDEASVARVR
                     AWVAERFAPMDPRPLHAEACLYTTTADERFVLERHGAVVVASACSGHGFKFVPWSGAR
                     AAELALRRS"
     gene            complement(8448..9596)
                     /locus_tag="VF058_10460"
     CDS             complement(8448..9596)
                     /locus_tag="VF058_10460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6208762.1"
                     /translation="MRAADVVIVGGGCVGASAAYHLTAGGAGSVLLLERADDLGTGST
                     AAAAGGFRYQFGSEVNVRLSLASIPMLIRFEEEHGIPLDVHQDGYLLLVRDEPSWEGF
                     RRAADMQRGLGVRVELLTGAEAAELIPGLVVDNVVGATFGPDDGIADTSGLTHGYATL
                     ARRAGAEIETGVDVAELLTEGDRVTGVRTEAGEVISAGAVVNAAGPWAGRLAITAGVD
                     LPLDPIPRNVVVTGPFPGAPERRTLVVDTPTSFYFHREGPGVLMGMGSRAEQPSFDIS
                     VDQTFVADEILPTAMRVFPPIEEAGIEHTWCGLYEMTPDRHPILGEAVRGLYLANGFS
                     GHGFQHSPIVGKLLAEMIVEGAASTVDVSSLSLDRFERGRPVVETHVI"
     gene            complement(9593..10525)
                     /locus_tag="VF058_10465"
     CDS             complement(9593..10525)
                     /locus_tag="VF058_10465"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6208763.1"
                     /translation="MTLSIVDATRLAELLPMGAAVDALEVAFGGTLPQAPLRSHVKVG
                     EADLLLMPAVERGGGAGVKLVTVNPENPDRGLPLIHGVYVLFAPGSLEPAAVVDGAAL
                     TGIRTAAVSGVATRHLARPDASRLAIFGAGTQARTHLEAMIAVRPIERLTVVSRTAER
                     AQDLVERARDLGLDARVGEPSDVREADVVCTCTTSPTPVFSGGELSDGVHVNAVGAYT
                     PDTRELDAETLRRGRVVVETREAALAEAGDLLLAIEEGAFSADAIVADLAEVVRGAEV
                     RTSDGDVTVFKSVGVAFEDLAVARAAVDRLEETE"
     gene            complement(10551..11303)
                     /locus_tag="VF058_10470"
     CDS             complement(10551..11303)
                     /locus_tag="VF058_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019433781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6208764.1"
                     /translation="MNDDGRRAASGTPLIELRGVYTSYGLVEVLRDITLEVREGEIAC
                     LLGANAAGKTTTLKTILGMVKPTAGEVYLRGERIDGENTQRIVAQGLTMVPEGRRLFG
                     KMSVRENLEIGTQLRKDEDGIQRDMERVLTLFPRLRERLDQKAGTLSGGEQQMCAIGR
                     ALMAGPKVLLMDEPSMGLAPILVEQVFEVIREINNQGTTIFLVEQNANMALSIAHRGY
                     VLQTGQVVMSDTAKTLRENPKTREAYLGELTE"
     gene            complement(11300..12091)
                     /locus_tag="VF058_10475"
     CDS             complement(11300..12091)
                     /locus_tag="VF058_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6208765.1"
                     /translation="MTDQLLVVKDLVKHFGGVRAVDGVDFHADEREIVSVIGPNGAGK
                     TTLFNVATGFYEADSGTIDFDGFNLLGLKPNRITALGIARTFQQIRLFPNLTVLENAM
                     VGQHARTRSGAFGAIFRPPWTRREEREIAERAREVLAFFGDSLLPYESMMAFELSYAD
                     RRRLEIARAMATHPKLLLLDEPTAGMNPVEKHGLVTLIGRLREEQGFTVVLIEHDMPV
                     VKGVSDRVVALDHGQVIAEGSYDEVSNNEQVIEAYLGKKKAVESA"
     gene            complement(12088..13926)
                     /locus_tag="VF058_10480"
     CDS             complement(12088..13926)
                     /locus_tag="VF058_10480"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6208766.1"
                     /translation="MAVPARATVRRDPNIRETSSWLNRSLVTTGLVGGAGALYFIAVG
                     MVEKFQALNVLTNVLALGRTLLIITFLVVGYVAARRLERQGHEAAGLRLSGGVVAGAV
                     AGAVAAVVLLFVDLIGVEHVRRMFQAFTPTLVNNVLSFGQSVMIGVLALLVGGAALGL
                     GGAGLQIFPPRLRRATTIGLLTALIVSLAHTFIIAVFRNLESNFGIDIPLTWLFTGTG
                     LAYSGLVLMFAAGFAFSWWRTRPEAAPETRRPEELTGERTLLDLVRGRPLRILGWLAV
                     AALLILMPFIVGQFLSQVINTVGIYVLLGLGLNIVVGFAGLLDLGYVAFFAVGAYATA
                     ILTSTASFLEGPFNFWVALPLVLLIAVFSGILIGAPVLRLRGDYLAIVTLGFGEIARV
                     FIASIWLQDFLGGAQGILRIPGPPFFGVNLRDPLNLYYIILAFCIIAAFVAWRLKESR
                     VGRAWAAMREDETVAEAMGISVINYKLLAFAIGAGVGCLGGAFFAIQVGSVFPNSMDL
                     LVSINALSVIVLGGMGSIPGVVLGAFGLVGLPELLREFEEFRLLIYGAVLVALMILKP
                     EGLLPEVRRRQELHERELEEEQYEDRVGEGAEPVLRAEASDDEETT"
     gene            complement(13926..15050)
                     /locus_tag="VF058_10485"
     CDS             complement(13926..15050)
                     /locus_tag="VF058_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6208767.1"
                     /translation="MADQTDTRPPEPGKTSPGGFQRGLMRVPVRSIILWILSIGLGIF
                     IIWGTSRTLSLEVSTFSGNGWKRLISDGVARGAIYALIAMGYTLVYGILFMINFAHGE
                     VFMSGAFTAFFMARWLAQTGFLNDNPVISLLILFAISMTVSMMLAVVLERVAYRPLRG
                     APRLVPLITAIGASLFLQNTFRGFYGASAEAYPTIDLLAGSIEILGWPMRKIQLVVVL
                     AAGALMLGLFLFIRKTRTGRAMRAVGEDKEIAALMGIDVDRTIMSTFAIGGLLAGAAG
                     ILFVYLFRGGAVNFFMGFFPGIKAFTAAVLGGIGNVVGAGVGGLLLGVLEAVGPFLLL
                     EGFNVPSPNQLQLVIAFSILVLVLIFRPGGLLGSPDEERA"
BASE COUNT         2197 a         5560 c         5241 g         2110 t
ORIGIN      
        1 cccaccgcct ccgacgctcc tcgatccctc agccggccgc cacgttcccg ccgggcggca
       61 ggtccgggta ccgaccgtag atctcctcgt acgtgccgtc ctcgatcatc tcggccagca
      121 ctcggttcag ctcctccagg agcggctcgt tgcccgggtt caccgcgatc ccgtagacct
      181 cgcccgtgtc gatcgtctgc acgagctcga ggccatcgcg ggcggcgatc tcggcgatcg
      241 ccgagggctc gtcgttgatc gcgccggtca cggctccgcc ctcgaccgcc gagtataggt
      301 cgggggtctg cgtgaaggtc cgaagcgtca ccccctgtgg ggcgaggttc tcctcggccc
      361 aggcctcgcc ggtcgtcccg cgctggaccg ccacggagtg ctcctcaccg agctcgtcca
      421 cgctggtgat cccgctgtcg gcgagcaccg tcagcgactg ctgggcgttg tagtagccct
      481 cggtgaagtt cacctcctcc tcacgctcgg gggtgatcgt ggtggccgag gccacgacgt
      541 cgaaccggcc tccggccagc tgcgtgaaga tcgtgtcgaa gtccgtgtcg atcacctcga
      601 cctcgagatc gagcctggtc gccatctcgg tgatcaggtc gatgtcgagc cccgtcagct
      661 cgccgccctc gcggaactcg aacggcggat acgggatgtc cgagcctacg gtcaagaccc
      721 cctcctggag cgtctcgatc gccggggccg tctcctcggt ttcggtcggc tcggccgtgg
      781 gctcggcgtc ggtgtcctcg tcgccgccac acgcgctcgc gacgagcatg agcgcggtga
      841 gcgccccgat cagccacagg taccgttgct tgcgcacgga tcccctccct tcatggattc
      901 attccctcgt tctcctgcga aatcctaggc aggccgcccg ctcatcacaa gcgagcgtag
      961 cgggagcccg gcgcggtgtg ccaggatgag cgcatgtcgt cctcgctggc gccgcgcgcc
     1021 gacttccccg tcctcgagga ggcggcgtac ctcaacgccg cctcgatcgc cctgatgccg
     1081 ctgcccgtcc gccggtcgat gcaggctttc atggaggaga tcgccgggcg cgggacgatc
     1141 ggcttcgacg acgacgccga ggcggccgtc tacgacgggg ttcgcagcag ggctgcgcgc
     1201 ctgctgggct ccgagccggc cgacgtcgcc gtcaccacga gcgcctccga ggcgctgggc
     1261 caggtggcgt ggtggctccg ccccggcgag ggcgagaacg tcgtctcgat cgacatcgag
     1321 ttccccagcg tgacgtacgc gtggatgcgg gtggcggagg agaccggcct cgaggtgcgg
     1381 ctggtgcgcg cgctcgagga tccgctccgg ctcacgctgg acagggtggc cgagctcgtg
     1441 gacgaccgga cggcggcgat cagcgtgagc cacgtgcagt acgcgaccgg ccacctgctc
     1501 gatctgcggg cgctggccga cctggcccac gcccacggcg cgcttgcggt ggtggacgcc
     1561 acgcagtcgg cgggggtggt cccgatcgac gtgcaggagg ccgacgtaga cgtgctcgtc
     1621 agcggggggt acaagtggct gtgcgccacg ttcggggcgg cgatctgcta cctgcaccca
     1681 cggatcgcgg agcggttcct gcctcccctg atcagctgga ggagcacgcc caaccccccg
     1741 gacttcgacg ccacgcggat gccgctcgcg gagggggcgc ggcggctcga gtactcgacg
     1801 gttgcctacc cctcgggcat cggtttgggc gaggcgatcg agtacatcct ggggctcggc
     1861 gtggaccgga tcctcgagca cgaccttggg ctcaccgaca aggtggccgc gggcctggag
     1921 gagctcgggg ggacgctgat ctcggcgccc cgggaggtgc cccgcggcag catcgccacc
     1981 gcccgattcc ccgggaggcg tcccgagcgg ctggtggaac ggctcatcca ggcgggcgtg
     2041 atcgccgggc cccggctcgg gggcgtccgc atctccccgc acctctacaa cgacgtctcc
     2101 gacgtcgacc gggccctcgc cgatctcgag cggatcctgg agacgcccga ggacgactga
     2161 ccggagcgga aggaaggcca tgagcggctc cctcgtgccc caggaccact tcccggtccc
     2221 cgacgggacg acctatctca actcggcgtc gatctcgctg acggcccggc ccgtccgcga
     2281 ggagatgcgc cgcttcgagg acgaggtcgt gcacctcggc acgatcaacc tcgacgacga
     2341 ggccgagaac cgcgtgtacg agggcgtccg aggctcggcc gcagcgctgc tcgggtgtga
     2401 cgccgacgac atcgccgtca tgacgagcgc cacggaggcc ctctgccagg tggcctggtg
     2461 gctccgcccc ggcgagggtg agaacgtcgt ctcgatcgac atcgagttcc ccagcgtgac
     2521 gtacccctgg ctgcggctgg cggaggacga ggggctcgag gtccggctgg tgcgggcgat
     2581 ggaggatccg cttcggctca cgctggaccg ggtggccgag ctcgtggacg agcggacggc
     2641 ggcgatctgc gtgagccacg tgcagtacgc caccggccat ctgctcgacc cgaccgcgct
     2701 gtccgagctg gcccacgcgc acgatgccct cctgatcctc gacaccacgc agtcatcggg
     2761 agccgtcccg ctcgacgtgc gctccaccga ccaggacgtc gtgatcgcca gcgcctacaa
     2821 gtggctcacg ggagtgccgg gcgccgcgtt cgcgtacctg cgaccggagg tcgccgagcg
     2881 gttccgcccg ccgttcgtcg gctggcggac gaccggcgag ggggcgatgg ccttcgacgc
     2941 cacgcgcatc tcgctggcgg agggtggccg gcggatggag tacgcgacgg tggcctacga
     3001 ggccgggatc tcgctgggcg cggcgacccg ctacctcctg gacgtgggga tcgagaagat
     3061 ccacgagcac gaccgtgggc tgtgcgccct cctggccgag gggctgaggg tccggggcgc
     3121 ggaaccgctc acaccggagg atcccgacgg ccgagggacg atcgtggcgg cgcgcttccc
     3181 gggacgggat ccgttcgagc tggccggcgc cctggcccgg cgaagcgtct tcgtcgctcc
     3241 tcgcctcggc ggcctccggt tctcgccgca cctccacaac gacgaggatg acgtgggccg
     3301 ggccctcgag gcgctcgacg ccgccctgga gggggcctga tgctcctgct gtccgaggag
     3361 gagatccgcc gctcggtctc gtcgaaggaa gccctggagg cggtggccga ggccttcgcc
     3421 gccctggccc gcggcgaggc gcgcctcccg ggggtgatct acctcgacct ccaggagttc
     3481 aacggagacg tgcacgtgaa gggagctcac atgcggggcg cctcgcactt cgccatcaag
     3541 gtggccgggg gttggtacga caacccgtcg aggggcctgc ccgtggggac ggggctcgtc
     3601 ctcgtgttca gcgcggagac gggggcgccg gcggcgctcc tgctcgacaa cgggtacctc
     3661 accgacctca ggacgggcgc cgccggcgcc ctcgccgcgc ggcatctggc cagggacccc
     3721 ctgtcgaagg tggccatgat cggtgccggg ctcgaggccc ggttccagct gcgggccctg
     3781 gccgacgtgc gggccctccc ccggatcgag gcctggagcc gtgaccccgc caacgcccgg
     3841 cggttcgccg aggagatgcg ggacgagctg ggggctgacg tcgaggtggc cccggacgtc
     3901 gagtcggccg tgcgggaggc cgatctcgtc gtcaccgcca ccccggcccg cgagcccctc
     3961 gtgcgtgccg actggctctc ctccggggtt cacgtcaccg ctctcggctc ggacggaccc
     4021 gccaagcagg agctcgacgt ggacgtcctg gcccgggccg acctcgtagt ggtggacgac
     4081 cttgaacagg ccgccgagaa gggcgagctt caccacgcgc tcgaggccgg cgccctgacg
     4141 cgcgaccgcg tggcggcgca gctcggagac ctcgtcagcg gcgaccaccc gggacgcacg
     4201 gacgacagcc agatcacggt cgcggacctc acgggcgtgg gcgtgcagga cgccgcggtc
     4261 gcatcgctgg cgctggagcg ggcggaggcg gcgggcctcg gccgcgacct ccccacctaa
     4321 ggtggacgtc cgcctcgacg accgggaccg cgagctcctc gacggggccc acggcgaggc
     4381 cggcagcctc gccatgcgca tcctcgtccg gatggcccgg gcgcaggggg cggcgggcct
     4441 ccgcgacgtg acgcgggccc acatcgacgg ctgcctctac cacgggcggg ccggcctgga
     4501 cttcgcccga cgcatggtgg acggaggcgg ccgcgtggcc gtcccgacca ccctcaacgt
     4561 ggcctcgctg gacctcctcc atcccgagcg ctaccggggc gatcccgaga cggcccgcca
     4621 cgcccgttcc ctgatggacg cctacgtgga gctcggatgc cggcccacgt ggacctgcgc
     4681 cccctatcag ctccccgagg cgaggccggc cctcggcgag cacgtggcct gggccgagtc
     4741 gaacgcgatc gtcttcgcca actcggtcct tggggcccgc accgatcgct acggcgactt
     4801 cctcgacatc tgcgcggcga tcaccgggcg cgtccccgag gcgggcctcc acctcgacga
     4861 gcaccgtcga ggcgagatca tgttccgcgt cgaggggatc ggctcggggc tgctgggcga
     4921 tccggtcctc taccccctcg tcggccacgt ggtggggagg gcgtcgggct cccgggtgcc
     4981 cgtcctcgtg ggcctgcccg gcgacgcctc cgaggacgac ctgaaggcgc tcggcgccgc
     5041 cgccgcgtcg tcgggctcgg tggcgctctt ccacgccgtg gggctgacgc ccgaggcgcc
     5101 cacgctcgac gaggcgctcg gcggccggcc cccgctccgc gaggagacga tcggccccga
     5161 acggctccgg gccgcgcggg aggagctcac gacctcccgc ggcgagcggc tgaccggggt
     5221 cagcctcggc accccccact tctcggtgga ggagttccgg gggctcctcg aagccctgga
     5281 aggggggcgg atccacccgg acgtcgagtg ctacgtctcg acggggaggg acgtcctggc
     5341 cgaggtcgag tcgaggggct ggctggcgcc catcgaggcc gccggcgtcc gcatcgtgac
     5401 cgacacgtgc acctacgtga ccccgatcat gccacccgga ggcgtggtga tgaccaactc
     5461 cgggaagtgg gcctactatg ccccggggaa cctcgggatc gccaccgtct tcggaagcct
     5521 cgaggagtgc gtgcggtcgg cgctggccgg gcgcgtcacc gtcgacgagg agctgtggcg
     5581 tggctgaggg acgggcgttg gcttcgggga tggccgaggg ggtcctgctc gtgctccggg
     5641 agccgctctc gttctggggc gggatggacc cggtgaccgg cgaggtggtc gacgcgcacc
     5701 accctcagcg cggcgaggtc ctctcggggc gcgtcgtggc catgccgtcg ggacgaggct
     5761 ccagcagcgc ctccagcgtc ctggccgagg ccatacggac cgggacggcc cccgccgccc
     5821 tggtgctcgg ggagccggac ccgatcgtcg ccctgggaag cgtcgtggcc tcggagctct
     5881 acgggtccgc ctgtcccgtg gtcgtgctgg acgacgcggc ctacgacctg ctgcgaaccg
     5941 gggaccggct cagggtcacc gccgacgagc acggggcccg cgtcgacatg cttgcctagc
     6001 ggccgttcaa gaggccgtcg gaccggaggt cccggaacgt ggcctgtgcc cccgagccca
     6061 gacgcacgat gctctgccct ccgaccatgg ccccgctccc cgagaccacg gtgttccgga
     6121 ccttcccggg gtccacgctc gtggtggcca gcagcagtcc caaggtgtcc tggaggctga
     6181 ggtccgtctg gaggttccgg gcgccgacgg ccacccaccg caccagcgcg gccgggtccc
     6241 tccgcagatc ggaccggaac tcccgcagcg tagccatcag cagccgtccc tggttcagcg
     6301 accgcccgaa gtcgccgccg ggggcgtcat gtcggttgcg ggagaacgct aacgcccccc
     6361 gacccccgag acgcctccgg ccgggacgga agaacgcctc ggaggccgcg tcgctcatcg
     6421 cgtaggggat cacggtccgt acgccgccca cgccggtcac cgcggcgcgg aacccctgga
     6481 agcccgtgag caggtaggcg ccgatccgga tgccgctcag ccgctccacc gtccgcaccg
     6541 tgagctccgg gcccccgtag aagagcgcct cgttgatctt gcgggtcccg acgccgggga
     6601 tcggcacgaa cgagtcgcga gggatcccca cgatgcccgc gctccccttc ctgaggttga
     6661 cgccgatgat gtgaagggag tctgcgcggg cgccggcgac gcgctccccg gggcgggcgt
     6721 cgctgccgat cgcaaggatg aagagcaccc cccggcgcgt cgccggcgcc cccatcggcc
     6781 gggccacctt cccggccgat gggatccgtc cccggacgtc gtaggacacg atccgccagc
     6841 gacccgccgc ccgtcggagc aggtacgacc cgcggtgccg gatctcgtcc tcgatcccgg
     6901 cccggaaggc cctcgccgcg aagcgcgccc gagccagcac ggcggtgggc cggcgcccgc
     6961 gatccgtgag gacccgcagg ttgagtcgag cgcggggcgc ccggagggtc gtgatccgag
     7021 acgaggcctt gcccagggtc aggacctcgc ggtcccggcg agcctgcttc cgcgtctgcg
     7081 ggacgaacag gtcgtcgacg cggggagacg ccccgcccgc gaccggctcc aggaaccccc
     7141 tcgtgtacag ctcctcgatc gtgcgctcga cgcctcgtcg ggcggcccgc aggcgcgggc
     7201 gccgggggcg cccgtggatc gtcacggccg acacccttcc cacccgcacg cggagcgccg
     7261 ggccgggcgg ctcgggcgag ggagacggcg acgccgtcgg gcccgacagg gagcggcggc
     7321 ccagctcggg ctcctggctc gggcctcccc gcacgaggaa cacgaacacc gctaccgtcc
     7381 cggcggccac gccggctatc agcttcgccc ggccggtcat gacctcctca aggcgagctc
     7441 ggcggctcgg gcgcccgacc aggggacgaa cttgaagccg tggccgctgc acgcggaggc
     7501 gacgaccacg gccccgtgcc gctctaggac gaaccgctcg tcggcggtcg tggtgtacag
     7561 gcacgcctcc gcgtgcagcg gcctcggatc catcggggcg aagcgctccg ccacccaggc
     7621 tcggacccgg gccaccgagg cttcgtcggg accgccccag tcgtcgggat cgacctcggg
     7681 cccggcgtgg tgctccgccg ccttcagccc gaaccccggg ctgggcagcg cgtaagtgag
     7741 atgctcgccg tgccagtcga cgacgacgac cggcggtggc tcggactcga agtaggctgg
     7801 ggactcgcgg gtgacgcgcg ccgggacggc gatgccgacc ggccggagga gatcgagggc
     7861 ccacgacccg gcggctacga cgccgacgcg cgccgagatc gttcccccat cggtgcgggc
     7921 ctccacccca tcctcgtccg ccctcagctc caccacctgg acgccctccc ggacctccgc
     7981 gcccgcccgg cgggctccct ccaccagcgc ccgccacgcc cggtcggcgt ggacgatccc
     8041 ggcgtcggcc tggaacagca cctcctcctc cggtgcgagg ctcagggagg ggaagcgctc
     8101 catcacggcg gcgccggaca gcacctcgta gcccatgccc tcggcatcga gcgacgtcgc
     8161 gttctcgggc ggggcgtcga ggtcgatccc gcccgtccgc gtcagcagct cctcccccgc
     8221 ctcatcctcg agctcggccc agagacccat cgcctccttc atcatgcgga cccagtccgg
     8281 agcgtcgtag gagtgtcgga agatccgcgc gtcgccgtgg ctcgagccgc ggtcgtggcc
     8341 gatccggaag cgctccaggg cgatcacgtc gagaccacgg cgggccagct cgcgagcggc
     8401 cgccgacccg acggcgcccg ccccgacgac gacgacgtcg gcgtccatca gatgacgtgg
     8461 gtctccacca cggggcgtcc ccgctcgaac cggtccaggg agagcgagga gacgtccact
     8521 gtggaggcgg cgccctccac gatcatctcg gccagcagct tccccacgat cggcgagtgc
     8581 tggaacccgt ggccgctgaa gccgttcgcc aggtacaggc cccgcacggc ttcccccagg
     8641 atcgggtgcc gatcgggcgt catctcgtag agcccgcacc acgtgtgctc gatccccgcc
     8701 tcctcgatcg gggggaacac gcgcatcgca gtgggcagga tctcgtcggc gacgaaggtt
     8761 tgatcgaccg aaatgtcgaa cgagggctgc tcggcccggg agcccatgcc catcagcaca
     8821 ccaggtccct cgcggtggaa gtagaagctg gtgggggtgt ccacgaccag cgtccggcgc
     8881 tccggggctc ccgggaacgg gccggtcacc accacgttgc gggggatcgg gtccagcggt
     8941 agatccaccc cggcggtgat cgcgagccgc ccggcccacg gtcccgccgc gttgacgacg
     9001 gccccagccg agatcacctc tcccgcttcg gttctcacgc ccgtgacccg gtctccctcg
     9061 gtgaggagct cggcgacgtc cactcccgtc tcgatctccg cccctgccct ccgcgccagc
     9121 gtcgcgtagc cgtgcgtgag ccctgaggtg tcggcgatcc cgtcgtctgg gccgaaggtc
     9181 gcccccacca cgttgtccac caccaggccc gggatcagct ccgcggcctc ggctccggtc
     9241 agcagctcga cgcggacgcc caggcctcgc tgcatgtcgg ccgcgcgacg gaacccttcc
     9301 cacgacggct cgtcgcgcac caggaggagg tacccgtcct ggtgcacgtc cagcgggatg
     9361 ccgtgctcct cctcgaaccg gatcagcatc gggatgctgg ccagggacag ccgcacgttc
     9421 acctcgctgc cgaactgata gcggaacccg ccggccgccg cggccgtgga gcccgtgccc
     9481 aggtcatccg ctcgctccag caggagaacc gaacccgccc ccccggccgt caagtggtag
     9541 gcggcgctgg cgcccacgca cccgcctccc acgatcacga cgtccgcggc cctcactcgg
     9601 tctcctccag ccgatcgacg gcggcgcggg ccaccgccag gtcctcgaag gccaccccca
     9661 ccgacttgaa gacggtgacg tcgccgtccg aggtgcggac ctcggccccc cggaccacct
     9721 ccgccaggtc ggcgacgatg gcgtccgccg agaacgcgcc ctcctcgatc gccagcagga
     9781 ggtcgccagc ctcggccagc gccgcctccc gcgtctccac cacgacccgc cctcgccgga
     9841 gggtctcggc atcgagctcc cgcgtgtcgg gcgtgtaggc acccacggcg ttcacgtgga
     9901 cgccgtcgga caattctcct ccggagaaga cgggcgtcgg gctcgtcgtg caggtgcaca
     9961 cgacgtcggc ctcgcgcacg tcggagggct cccccacgcg agcgtcgagt cccagatcac
    10021 gggctctctc cacgaggtcc tgggctcgct ccgccgttcg cgacaccacg gtgagtcgct
    10081 cgatcgggcg gacggcgatc atcgcttcga ggtgggtccg ggcctgtgtc cccgccccga
    10141 agatcgcgag gcgcgaggcg tcgggccggg cgaggtggcg cgtggcgacc cccgagaccg
    10201 ccgccgtccg gatgcccgtg agcgccgcac cgtccacgac cgccgcgggc tccagcgagc
    10261 ccggggcgaa caggacgtac acgccgtgga tcagcggcaa ccctcggtcc gggttctccg
    10321 gattcaccgt gacgagcttc accccggctc ccccacccct ctccaccgcg ggcatcagga
    10381 gcaggtcggc ctcccccacc ttcacgtgcg accggagcgg ggcctggggg agggtcccgc
    10441 cgaacgccac ctcgagcgcg tccacggccg cgcccatcgg cagcagctcg gccaggcgtg
    10501 tcgcatcgac gatggagagg gtcacggggg cggcgggacc ttccgacgca ctactcggtc
    10561 agctcgccga ggtaggcctc gcgggtcttg gggttctccc tcagggtctt ggcggtgtcg
    10621 gacatgacca cctgaccggt ctgcaggacg tacccgcggt gggcgatcga gagggccatg
    10681 ttcgcgttct gctccacgag gaagatcgtg gtcccctggt tgttgatctc ccggatcacc
    10741 tcgaagacct gttccacgag gatcggggcc aagcccatcg agggctcgtc catcaggagg
    10801 accttgggcc cggccatgag cgcccgcccg atcgcgcaca tctgctgctc gccccccgag
    10861 agcgtccccg ccttctggtc gaggcgctct cggagccgcg ggaacagggt caggacccgc
    10921 tccatgtcgc gttggatccc atcctcgtcc tttcggagct gggttccgat ctcgaggttc
    10981 tcccggaccg acatcttccc gaagagccgc cgcccctcgg gcaccatcgt cagcccctgc
    11041 gccacgatcc gctgggtgtt ctcgccgtcg atccgctcgc cccgcaggta gacctcgccg
    11101 gccgtcggct tcaccatgcc caggatcgtc ttgagcgtcg tcgtcttccc cgcggcgttc
    11161 gctcccagca ggcaggcgat ctcgccctcg cgaacctcca gcgtgatgtc ccggagcacc
    11221 tccacgagcc cgtagctcgt gtacaccccg cgcagctcga tgagcggggt ccctgaagcc
    11281 gcccgccgac catcgtcgtt cacgccgact ccaccgcctt cttcttcccc aggtaagcct
    11341 cgatgacctg ctcgttgttg gagacctcgt cgtacgatcc ctccgcgatc acctggccgt
    11401 ggtcgagggc cacgacgcgg tccgacaccc ctttcaccac gggcatgtcg tgctcgatga
    11461 ggaccacggt gaagccctgt tcctcgcgca gccgcccgat cagcgtgacg agcccgtgct
    11521 tctcgaccgg gttcatgccg gccgtcggct cgtcgagcag gaggagcttg gggtgcgtgg
    11581 ccatggcccg ggcgatctcc aggcgccggc gatccgcgta ggacagctcg aacgccatca
    11641 tgctctcgta cggcagcagg ctgtcgccga agaacgcgag cacctcgcgc gcccgctccg
    11701 cgatctcgcg ctcctcccgt ctcgtccacg ggggacggaa gatcgccccg aaggcccccg
    11761 agcgggtccg ggcgtgctgc cccaccatgg cgttctcgag cacggtcagg ttggggaaca
    11821 agcggatctg ctggaacgtc ctggcgatcc cgagcgcggt gatgcggttg ggcttcaggc
    11881 ccagaaggtt gaagccgtcg aagtcgatcg tgccgctgtc ggcctcgtag aagccggtgg
    11941 cgacgttgaa cagcgtggtc ttgccggcgc cgttggggcc gatcacgctg acgatctccc
    12001 gctcgtcggc gtggaagtcc accccgtcca ccgcgcggac ccccccgaag tgcttcacca
    12061 ggtccttgac gaccagcagc tggtcggtca cgtcgtctcc tcgtcgtcgg atgcctcggc
    12121 ccggagcacc ggctcggccc cctcgcccac ccgatcctcg tactgctcct cctcgagctc
    12181 gcgctcgtgc agctcctgcc gcctgcggac ctcgggcagg agcccctcgg gcttgaggat
    12241 catcagggcc acgaggacgg cgccgtagat caggagccgg aactcctcga actctcggag
    12301 gagctcggga aggccgacga gcccgaacgc gccgaggacg accccgggga tgctccccat
    12361 gcctccgagg acgatcacgg acagcgcgtt gatcgagacg aggaggtcca tcgagttcgg
    12421 gaacaccgac cccacctgga tggcgaagaa ggcgccaccc aggcagccca ccccggcgcc
    12481 gatcgcgaac gccagcagct tgtagttgat cacgctgatg cccatcgcct cggcgacggt
    12541 ctcgtcctcg cgcatcgccg cccacgctcg tcccacccgg gattccttca gccgccaggc
    12601 gacgaacgcg gcgatgatgc agaaggccag gatgatgtag tagaggttca gcgggtcgcg
    12661 caggttgacg ccgaagaacg ggggtccggg gatgcgcagg atcccctgcg cccccccgag
    12721 gaagtcctgc agccagatcg acgcgatgaa cacccgggcg atctccccga acccgagggt
    12781 gacgatcgcc aggtagtcgc ctcgcagcct caggaccggg gcgccgatca ggatgccgct
    12841 gaagaccgcg atcagcagga ccagcggcag ggccacccag aagttgaacg ggccctccag
    12901 gaacgaggcg gtggaggtca ggattgccgt ggcataggcg ccaaccgcga agaacgccac
    12961 gtagccgagg tccagcagcc ccgcgaaccc gacgacgatg ttgaggccga gcccgaggag
    13021 cacgtagatc ccgaccgtgt tgatcacctg gctgaggaac tggcccacga tgaacggcat
    13081 caggatcagc agggccgcca cggccagcca gcccaggatc ctgagcgggc ggccccggac
    13141 gagatcgagc agcgtccgct cccccgtcag ctcctcgggc ctcctggtct cgggagccgc
    13201 ttccggccgg gtccgccacc aggagaacgc gaacccggcc gcgaacatca ggaccagccc
    13261 cgaataggcg agaccggtcc ccgtgaacag ccacgtgagc gggatgtcga tgccgaagtt
    13321 cgactcgagg ttgcggaaca ccgcgatgat gaacgtgtgc gccaggctca cgatgagggc
    13381 ggtgagcagg ccgatcgtgg tggcgcggcg caggcggggc gggaagattt ggagcccggc
    13441 tcccccgagc ccgagcgcgg cccccccgac cagcagggcc agaacgccga tcatcacgct
    13501 ctgcccgaag gacaggacgt tgttgacgag ggtcggcgtg aaggcctgga acatccgccg
    13561 gacgtgctcc accccgatca ggtcgacgaa cagcaagacg accgccgcga cggcgcccgc
    13621 gacggcgccc gccacgacgc cccccgagag acgcagcccc gccgcctcgt ggccctggcg
    13681 ctcgagcctc ctcgcggcca cgtacccgac cacgaggaag gtgatgatga ggagcgtcct
    13741 gccgagcgcg agcacgttcg tgaggacgtt gagggcctgg aacttctcca ccatccccac
    13801 ggcgatgaag tacagggcgc ccgcgccgcc cacgagcccg gtcgtgacca ggctccggtt
    13861 gagccaggac gacgtctccc tgatgttggg gtctcgccgt accgtcgccc tcgcggggac
    13921 cgccatcacg cgcgctcctc gtccggagag ccgagcagcc cgccgggccg gaagatcagc
    13981 acgagcacca ggatgctgaa cgcgatcacg agctggagct ggttcggcga cggcacgttg
    14041 aagccctcca ggaggaggaa gggccccacc gcctctaaga ccccgagcag cagccccccg
    14101 acgccggcgc ccacgacgtt cccgatgcct ccgaggaccg cggccgtgaa cgccttgatc
    14161 cccgggaaga agcccatgaa gaagttcacc gcgccgccgc ggaacaggta cacgaagagg
    14221 atcccggccg ccccggccag gaggcccccg atggcgaagg tgctcatgat ggtacggtcc
    14281 acgtcgatgc ccatgagggc cgcgatctcc ttgtcctcac ccaccgcgcg catcgcccgc
    14341 ccggtcctcg tcttcctgat gaagaggaac aggcccagca tcagcgcccc ggcggcgagg
    14401 accaccacga gctgaatctt cctcatgggc cagccgagga tctcgatcga tcccgcgagg
    14461 aggtcgatcg tggggtacgc ttcagcgctg gcgccgtaga accctcggaa ggtgttctgg
    14521 aggaacagcg aggccccgat cgcggtgatg agcggcacga gcctgggagc gccccgcagc
    14581 ggccggtagg cgacgcgctc gagcacgacc gcgagcatca tcgagaccgt catcgagatc
    14641 gcgaagagga tcagcagcga gatgaccggg ttgtcgttca ggaaccccgt ctgcgcgagc
    14701 cacctcgcca tgaagaacgc ggtgaaggcc ccgctcatga acacctcgcc gtgggcgaag
    14761 ttgatcatga acaggatgcc gtacacgagc gtgtagccca tcgcgatcag cgcgtagatc
    14821 gcgccccggg ccacaccgtc gctgatgagc cgcttccagc cgttgccgct gaacgtgctc
    14881 acctcgagcg agagggtgcg gctcgtcccc cagatgatga agatccccag gccgatcgag
    14941 aggatccaga ggatgatcga tcggaccggc acccgcatga ggccccgctg gaagccgccg
    15001 ggcgacgtct tacccggctc ggggggtctg gtgtccgtct ggtcggccat cagcgctcgt
    15061 caaggtctgg gggagcggcc gcgggtgagc cggactggct cacccgcg
//
LOCUS       DASUTW010000304         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1009090, whole genome shotgun sequence.
ACCESSION   DASUTW010000304 DASUTW010000000
VERSION     DASUTW010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009090"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VF058_10490"
     CDS             complement(<1..495)
                     /locus_tag="VF058_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEX6208768.1"
                     /translation="MGHRAAVLTVSDGVSRGVREDASGDAVEALLRDAGYAVRRGVVP
                     DERTEIETVLRELSAGAELVVTTGGTGLGPRDATPEATRAVIEREVPGLAEAMRATGR
                     ASTPMADLSRGVVGSVGASLIVNLPGSPAGAVESLGAVLPVLPHALDLLAGRGEHEPS
                     GTRRG"
     gene            560..1708
                     /locus_tag="VF058_10495"
     CDS             560..1708
                     /locus_tag="VF058_10495"
                     /inference="COORDINATES: protein motif:HMM:NF028293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HEX6208769.1"
                     /translation="MDRRLVGDAAGSLADLGVLVPLAAALILVNGLDPGAVLIGAGVL
                     VLAAGVAFRVPFPVQPLKALAAVAVASRVAPEVIHAAGLEIALVLLLLSFRGVADRVA
                     RVFTKPVVRALQVGVGMLLVLASARLVLDPPAVFRGTPPSPWPALLAVAAFGGLWWAA
                     RAGRYEVALGVLAGGALAAWLAAGAELAPPSLHLPSLSLPSGGAFGEALVLLVIPQLP
                     LTFGNAVVAVDDLAHEYFGPRADRVTPSRVCLSCGLGNVVAGLIGGMPMCHGAGGLTA
                     HVRLGARTARMNLLLGGALLALGLFFAPQAPAILGLLPVWALAALLALAGLRHALLAA
                     DLRGASLAVAVVAGSIGALVGNLTVTLAVALVGAHAWRAVPRGQAPRS"
     gene            complement(1690..1881)
                     /locus_tag="VF058_10500"
     CDS             complement(1690..1881)
                     /locus_tag="VF058_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208770.1"
                     /translation="MADMGEAQRELAEIYERLKTVLSERGDELAPYEERNALKALAAL
                     WRVANGLDLDPGQLYDLGA"
     gene            complement(1874..>2414)
                     /locus_tag="VF058_10505"
     CDS             complement(1874..>2414)
                     /locus_tag="VF058_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HEX6208771.1"
                     /translation="YVEEVIDPETGGPVDYGEQGERVVTSFGRGFIPVLRYRTRDLVV
                     KVPGAGCACGRTFDLYDGGIRGRVDDMKLVRGTNVYPRAVEAIVREIPEIDEFQIHLY
                     TAEGIRDEIEVLVEIPDEGADVDGILARLGKELADAHESLRFGVQRAETGSLPRFELK
                     AKRLVDEREVIGGEGERRG"
BASE COUNT          242 a         1005 c          805 g          362 t
ORIGIN      
        1 gccccgcctc gtcccgctgg gctcgtgctc cccccggccc gcgaggagat cgagggcgtg
       61 cggaagaacc ggcaggacgg cccccaggct ctccacggcc ccggcggggc tccccggcag
      121 gttcacgatc agcgaggccc ccacggaccc gaccaccccg cgggacaggt ccgccatcgg
      181 ggtcgaggcg cggcccgtcg cgcgcatcgc ctcggccagg cccgggacct cccgctcgat
      241 cacggcgcgc gtggcctcgg gcgtcgcgtc ccgcggtccg aggccggtgc ccccggtcgt
      301 cacgaccagc tccgccccgg cggagagctc ccgcagcacc gtctcgatct ccgtgcgctc
      361 gtcgggcacg acgccccggc gcacggcgta gcccgcgtcg cgcagcagcg cctcgacggc
      421 gtctcccgag gcgtcctccc ggacgcctcg ggagacgccg tcgctcacgg tcagcaccgc
      481 ggcccgatgt cccatcgcgc tcatgatagg cagggatccc gcgacgcgcg ccgagcgacc
      541 tcgtgccaga ctcgagccca tggaccggcg cctcgtgggc gacgccgcgg gaagcctcgc
      601 cgatctcggc gtgctcgtcc cgctggccgc ggccctcatc ctcgtcaacg gcctcgaccc
      661 cggggccgtg ctgatcgggg ccggcgtcct cgtgctcgcc gccggcgtcg ccttccgggt
      721 cccgttcccc gtccagcccc tcaaggctct ggccgcggtg gcggtcgcct cgcgggtggc
      781 gcccgaggtc atccacgcgg cgggcctgga gatcgccctc gtgctgctcc tgctctcgtt
      841 ccgcggggtc gccgatcgcg tggcccgcgt gttcacgaaa cccgtggtcc gtgcgctgca
      901 ggtcggggtc gggatgctcc tggtcctggc ctcggcgcgc ctcgtcctcg accccccggc
      961 ggtgttccgg ggcacgccgc cctcgccctg gcccgccctg ctggccgtcg cggcgttcgg
     1021 aggcctctgg tgggcggccc gggccgggcg ctacgaggtg gccctggggg tcctggccgg
     1081 gggcgcgctg gccgcctggc tcgcggcggg cgcggagctc gcccctccct cgctccacct
     1141 gccctcgctc tcccttccct ccgggggcgc gttcggggag gcgctcgtgc tgctcgtgat
     1201 ccctcagctc ccgctcacgt tcgggaacgc ggtcgtggcc gtggacgacc tggcccacga
     1261 gtacttcggg ccgagagccg accgggtcac cccctcgcgc gtctgcctct cctgcggcct
     1321 ggggaacgtc gtcgccggcc tgatcggcgg gatgcccatg tgccacggcg ccggaggcct
     1381 caccgcgcac gtccgtctcg gcgcgcggac cgcccggatg aaccttctcc tcgggggagc
     1441 gctcctcgcc ctgggcctct tcttcgctcc gcaggcccca gcaatcctgg ggctgctgcc
     1501 cgtctgggcg ctggccgcgc tcctggcgct ggccggcctc cgccacgcac tgctcgcggc
     1561 ggacctgcgc ggcgcgagcc tcgcggtcgc cgtggtcgcg ggatcgatcg gcgcgctcgt
     1621 gggaaacctc acggtgacgc tcgcggtcgc gctcgtcggc gcgcacgcct ggcgcgccgt
     1681 gccgcggggt caggcgccca ggtcgtagag ctggcccgga tccaggtcga gcccgttcgc
     1741 cacccgccac agcgcggcca gcgccttcag cgcgttccgc tcctcgtagg gcgcgagctc
     1801 gtccccgcgc tcggacagca ccgtcttcag ccgctcgtag atctcggcca gctcgcgctg
     1861 ggcctcaccc atgtcagcca cgccgctccc cctcgccgcc gatcacctct cgctcgtcca
     1921 cgagcctctt ggccttcagc tcgaagcggg gcagggaccc cgtctccgcc cgctgcacgc
     1981 cgaaccgcag cgactcgtgc gcatcggcca gctccttccc cagcctggcc aagatcccgt
     2041 ccacatcggc cccctcgtcg gggatctcca ccaggacctc gatctcgtcc cggatcccct
     2101 ccgccgtgta gaggtggatc tggaactcat cgatctcggg gatctcgcgg acgatcgcct
     2161 ccaccgcccg cgggtagacg ttcgtccccc gcacgagctt catgtcgtcc acgcgccccc
     2221 ggatgccgcc gtcgtagagg tcgaaggtgc ggccgcatgc gcatcctgcg cccggcacct
     2281 tcaccaccag gtcccgcgtc cggtaccgca gcaccgggat gaacccccgc ccgaacgagg
     2341 tcaccacccg ctctccctgc tccccgtagt ccaccggccc ccccgtctcc gggtcgatga
     2401 cctcctcgac gtag
//
LOCUS       DASUTW010000305         8856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1138517, whole genome shotgun sequence.
ACCESSION   DASUTW010000305 DASUTW010000000
VERSION     DASUTW010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8856
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138517"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VF058_10510"
     CDS             complement(<1..321)
                     /locus_tag="VF058_10510"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF012915.4"
                     /note="binds the polymerase to DNA and acts as a sliding
                     clamp; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX6208772.1"
                     /translation="MKFRCERDTLAEALATAQRGVSTRPGIPALTGVMLEAGSEGLTV
                     TTTDLEVTTQVHVPVEVSEEGSALVPARLVAEMVKSLPPDDVDISADGSIARVTCRSF
                     EGTLR"
     gene            complement(518..1903)
                     /gene="dnaA"
                     /locus_tag="VF058_10515"
     CDS             complement(518..1903)
                     /gene="dnaA"
                     /locus_tag="VF058_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX6208773.1"
                     /translation="MWKEAVENLPGGLSDPDAHDLWTRAAGAARGRVSPSTYDMWFAD
                     VRGRRLEGDTIELATPTEYARAWLSSHHEELIRSAVTEAAGRPLDVRLVVEERPEAPP
                     EAPDERPASEDVPPYQRFPDRYTFETFVLGPSNRFAHAAAMAVAEAPPSKAYNPLFIY
                     GGVGLGKTHLLYAIATHMSKLIEGVRVKYVTSEAFVTEFIRAVRERQGYQFQRRYRDV
                     DVLLVDDIQFLARAEETQTEFFHTFNALHGSGSQIVIASDRPPQELAGLEERLRNRFR
                     WGLCVDVQPPDLETRIAILQKKAERDHMEVPDAVQHFIANKFDMNIRELEGALLRVAA
                     FASLSGVKVDLPLAERALEDLIPQTGQEIPPELIMEQSAQYFSLRRSDLVSKSRSRPL
                     TTARHIAMYLLRELSGLSLIKIGELFDRDHSTALHGIKKIEALMPARGSTYRQVQELT
                     KTVRSLARGGG"
     gene            2115..2249
                     /gene="rpmH"
                     /locus_tag="VF058_10520"
     CDS             2115..2249
                     /gene="rpmH"
                     /locus_tag="VF058_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX6208774.1"
                     /translation="MKRTYQPSNRRRKRRHGFRTRMRTRGGRAILARRRAKGRARLSA
                     "
     gene            2278..2556
                     /gene="rnpA"
                     /locus_tag="VF058_10525"
     CDS             2278..2556
                     /gene="rnpA"
                     /locus_tag="VF058_10525"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX6208775.1"
                     /translation="MGGRPLRSRRLTLYLAPTDGGPRTAVVVGRSVGGAVRRNRAKRL
                     IREVWKTVGPRLRESADAVLVARPEIEGATTTELIEEVEDVLTRAGVL"
     gene            2553..2828
                     /gene="yidD"
                     /locus_tag="VF058_10530"
     CDS             2553..2828
                     /gene="yidD"
                     /locus_tag="VF058_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX6208776.1"
                     /translation="MRRALWWAGLPARTLLLGLVRGYRRLFRGAYAGRCRFHPSCSAY
                     AEEAIRVHGAFKGVGLALWRVLRCSPLTPGGVDQVPPRRDGVIQGPA"
     gene            2873..3976
                     /locus_tag="VF058_10535"
                     /pseudo
     CDS             2873..3976
                     /locus_tag="VF058_10535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
     gene            3942..4592
                     /gene="jag"
                     /locus_tag="VF058_10540"
     CDS             3942..4592
                     /gene="jag"
                     /locus_tag="VF058_10540"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEX6208777.1"
                     /translation="MEEVERNAASVEEAVEAALEELGITEQEAQVEVLQEPQKGLLGI
                     GSKGAVVRVRRRTLLGQATEEQLRVAIDFLAGLMERLGVKAEVEEETHENVQYLDVVA
                     AGDPDAMGLLIGRHGQTLDAIQELTRAAVQRRTAERCRIVVDVEDYRKRRRAQLVERA
                     REAAEKVQRSGRRHTMEPMTPFERKIVHDVVAEFPGLESGSEGQEPKRRVVIRKRR"
     gene            4626..5192
                     /gene="rsmG"
                     /locus_tag="VF058_10545"
     CDS             4626..5192
                     /gene="rsmG"
                     /locus_tag="VF058_10545"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX6208778.1"
                     /translation="MKRLDEYEELLRDRAIPMGLIAASDAEGLRERHIDDSLRATRAF
                     RDDDACCIDVGSGAGLPGIPLAIALPDREFVLIESRRRRVGFLELAIDLLELANVRVL
                     PDRVESVGERVRAGLMAPADLATARAFGPLERSWRSAAPLLRPGGRLVYFGGRGLEDP
                     VGTARSAGVDGEISVLDSSSPLVIMGRR"
     gene            5270..6337
                     /locus_tag="VF058_10550"
     CDS             5270..6337
                     /locus_tag="VF058_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX6208779.1"
                     /translation="MEPPAEPPGPEREGEPAERPTEPASPIGPSAASPNPQVRVEGSL
                     EQPPAEAGGRIIAVANQKGGVGKSTTAVNLGAALAESGARVLVVDMDPQGNASTGLGI
                     SPQDREITTYQVLAAGLPAARAIMATAVEGLSAITSTIDLAGAEIELVSQFSREMRLA
                     RALDGVVEGYDYVIVDCPPSLGLLTVNSLAAADELIVPIQCEYYALEGLGQLLRNVRL
                     VQQNLNPRLRLTGIVLTMYDSRTKLAEQVVAEVRKYFGRRVYDTLIPRSVRLSEAPGY
                     GQPITTYDPGSKGAEAYRALASEVAQGPREVETPMPTEDMPEVVLPEPSPEDLDGEDE
                     QEPEPAVKRRRGLFRRKGDES"
     gene            6334..7179
                     /locus_tag="VF058_10555"
     CDS             6334..7179
                     /locus_tag="VF058_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011607911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX6208780.1"
                     /translation="MKQRGGLGRGLSALIPGATEEGGLLEIPLAAIAPNTRQPREAFD
                     EEGIAALASSIQEVGVLQPIVVRRREDGYELVAGERRLRAAKVAGLATIPAVIRDTDD
                     TDSLREAIIENIHREDLSPLEQAAAFQELLEDLGASHDELAERLGYSRSHVTNTIRLL
                     QLPPPVQRLVGEGRLSAGHARALLALDDVEGQEALALRVAAEGLSVRQVEELVRTYRG
                     GGERGAERAAPEPDPRAVQVEELLGDALATRVRIQRGRRKGKIVIEFGSDDDLDRIVS
                     AIAGS"
     gene            7270..7830
                     /locus_tag="VF058_10560"
     CDS             7270..7830
                     /locus_tag="VF058_10560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6208781.1"
                     /translation="MPRVVDVGLENLSSAPPKVLECAFWETEREDPADPRFYKEEWFS
                     STLLEWGSCGKLLVDEDEVLAFAQFAPPSLFPRVAGYRAGRVSPDAVYLAYCYVTTGH
                     RGRGLGSHMIRAVARDLVDRGFRAVESLGQSEWEGGWVLPEAFLAANRFAVVRPDPAV
                     PLMRLELFGVATPAGARAAEALPLPR"
     gene            complement(7827..>8856)
                     /locus_tag="VF058_10565"
     CDS             complement(7827..>8856)
                     /locus_tag="VF058_10565"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX6208782.1"
                     /translation="ARLLALGYTISQSELEEDRFEGSTDAAVRAFQQDRGLLVDGLVG
                     PQAWEELVEAGYALGDRVLYLRHPPFRGDDVRALQARLNTLGFDPGREDGIFGRQTEV
                     AMRDFQRNVGLPSDGIVGSTSLQALERLRASVPGPGRTLVRETEDLRLAGSLEGKTVA
                     IDPAHGPGDPGEVGPGGSVESEITYAIAERLAEALGGRGAKAVLLREPGEDPTVAARI
                     DRANASGADLLLSIHLNHHEDPTAEGAAAYYFGRLGAVSVAGRALAEVLLERITSRLG
                     LRDGRAHPKAFPLLRETPMPAVHLEPGFISNPREERLLSEGRFHHDLAEAIVEALERY
                     VAGLAGSD"
BASE COUNT         1299 a         2971 c         3312 g         1274 t
ORIGIN      
        1 gcgcagggtg ccctcgaagc tgcggcaggt cacccgggcg atcgagccgt ccgccgagat
       61 gtcgacgtcg tccgggggca gggacttcac catctccgcg acgagcctcg ccggcacgag
      121 ggcgctcccc tcctcggaga cttccaccgg gacgtgcacc tgcgtcgtga cctcgagatc
      181 ggtcgtggtg acggtgaggc cctcgctgcc cgcctcgagc atcacccccg tcagcgcggg
      241 gatccccgga cgggtcgaga ccccccgctg cgcggtcgcc agcgcctcag ccagcgtgtc
      301 gcgttcacac ctgaacttca caggccacct cccgctcttg agaaacgctc tcttcctttc
      361 caaaccagtg acagtcgtag gggctgtgga actgtggaga actcatcgtc cgcccagggt
      421 agcgcggggg tctgacatgg gacgccctgg ggacgggctc gggatgaggc gcgcgtccgg
      481 gcgccgcccg ccgccacgcc gcgatcttct ccacaactca tcctccacct ctggccagcg
      541 accggaccgt cttcgtgagc tcctgtacct gcctgtaggt cgagccgcgg gcgggcatga
      601 gggcctcgat cttcttgatc ccgtggagag cggtcgagtg atcgcggtcg aacagctctc
      661 cgatcttgat cagggagagc cccgagagct cgcgcaggag gtacatggcg atgtgccgag
      721 cggtggtcag gggacgagat ctgctcttgg acaccagatc cgagcgccgg aggctgaagt
      781 actgggccga ctgctccatg atcagctccg gggggatctc ctgccccgtc tgggggatca
      841 ggtcctcgag agcgcgctcg gcgagcggca ggtccacctt cacgcccgag agcgaggcga
      901 aggcggcgac ccgcagcagc gccccctcga gctctctgat gttcatgtcg aacttgttgg
      961 cgatgaagtg ctggacggcg tcgggcacct ccatgtggtc ccgctcagcc ttcttctgga
     1021 ggatggcgat ccgcgtctcg aggtcggggg gctggacgtc cacgcagagc ccccagcgga
     1081 accggttgcg aagacgctcc tcgagccccg ccagctcctg gggagggcgg tcgctggcga
     1141 tgacgatctg gctccccgag ccgtgaagcg cgttgaacgt gtggaagaac tcggtctggg
     1201 tctcctcggc ccgggccagg aactggatgt cgtcgacgag aaggacgtcg acgtcgcggt
     1261 agcgccgctg gaattggtag ccctgccgct cccggacggc acggatgaac tcggtgacga
     1321 aggcctcgga ggtgacgtat ttgacgcgca cgccctcgat cagcttgctc atgtgggtgg
     1381 cgatcgcgta gaggaggtgg gtctttccca gccccactcc cccgtagatg aagagcgggt
     1441 tgtaggcctt ggagggcgga gcctcggcca ccgccatcgc cgcggcgtgc gcgaaccggt
     1501 tcgacgggcc gaggacgaag gtctcgaacg tgtagcggtc ggggaagcgc tggtacgggg
     1561 ggacgtcctc ggaggccggc cgctcgtcgg gggcctccgg cggggcctcg ggccgctcct
     1621 ccaccacgag ccggacgtcg agggggcggc ccgcggcctc cgtcacagcg ctccggatca
     1681 gctcctcgtg atgggacgac agccaggccc gggcgtactc ggtgggggtg gccagctcga
     1741 tcgtgtcgcc ctccagccgg cggccgcgca cgtcggcgaa ccacatgtcg taggtgctcg
     1801 gcgagacccg tccgcgcgcg gcgccggccg ctctcgtcca caggtcgtgg gcgtcggggt
     1861 cgctcagccc tcccgggagg ttttccacag cctctttcca catgtgtgaa atccaccggg
     1921 gaagtgcaac ggggccgccg gaaggcgacc ccagggaagc gacgcggata ttagcacccc
     1981 cctcacgccg aaagcaatgg ggcgccgctg acctgcggct tcgtagccgc gccccggggt
     2041 ccggcgggca tcgccgcagg tcagcggcgc ccacgtggac cggccgggtc cacggtgcgt
     2101 atactcccgt attcgtgaag cgcacctatc agccgagcaa ccgcagacgc aagcgccgcc
     2161 acggcttcag gacccgtatg cgcacccggg gcggccgcgc gatcctcgcc aggcgacggg
     2221 cgaagggccg tgcccgcctc agcgcgtagc ctcaggggga gcgagatccg ggggctgatg
     2281 ggcgggaggc cgctgcgctc gaggcgcctc acgctgtacc tggctccgac cgacgggggc
     2341 ccccgtacgg ccgtcgttgt cgggaggtcg gtgggcgggg cggttcggag gaaccgggcg
     2401 aagcggttga tccgggaggt atggaagacg gtgggcccga ggctgaggga gtccgccgat
     2461 gcggtcctgg tggcgcggcc ggagatcgag ggggccacga cgacggagct gatcgaggag
     2521 gtcgaggacg tgctgacgcg ggcgggcgtg ctgtgaggcg ggccctctgg tgggccggcc
     2581 tgcccgctcg aaccctcctc ctgggcctcg tccggggcta ccggagactc ttccgcgggg
     2641 cctacgcggg gcggtgccgc ttccacccgt cttgctcggc gtacgccgag gaggcgatcc
     2701 gggtgcacgg ggccttcaag ggagtcggcc tggccctgtg gcgcgtgctc cgctgcagcc
     2761 ctctcacccc gggcggtgtg gaccaggtac ccccgaggcg tgacggggtc atacaggggc
     2821 ccgcctgatg gagcagatcg ccggcgttct cctgcagacc ctgggctccg cgctcagctt
     2881 cctctacgac ctgatcccgg tgtacggcgt ggcgatcgtg ctgctcacga tcgccgtgcg
     2941 gctgctgctg ctccccctca ccatcaagca gacccgctcc atgcaggcga tgggacgcct
     3001 ccagcccaag gtgaaggagc tccagcggaa gtacaagggc gaccggcaga agctgaacga
     3061 ggagatgatg aagctctacc gggagcacgg cgccaacccg ctgggcggct gcctccccct
     3121 cctcctccag ctcccggtct tcttcgccct ctacgcggtg ctgcgggcgg ccacgcccga
     3181 gatcgcgctc cccgcggagc cggtctcctt cgggaccgtg gaggacgcgt tctgccggcc
     3241 ggtgggcgag ccgggcatca ccgggacggc cccgaacgag ctggagtgcg acacggacgg
     3301 cgacgggaac ttcgagaggt cgatcccgat ctccgggtgg gaggagcggg acggggaggc
     3361 gatccggccc cgggacgtcc tgacccgatg caccccacag agcgagggcg gtgaggtgcg
     3421 gttccgctgc gagacgccgg tggggacggg ccacctcccc gaggacgggg ccctcttcga
     3481 ggcgatcatc gaggacggcg agccgttcct ggggatgtac ctctcatgct cggccacaca
     3541 ggcccagtcg gaggaggcct tccggcagtg ctcgcccagg ggggccgaag ggagcgctct
     3601 gaacctggtg ggatattatg ggttgatcgc cctgatggtc ctctccacct tctaccagac
     3661 gaggcagatg caggcggttt ccgggagcca gcagccggga gcccagacga tcgggcggat
     3721 catgccgatc ttcctggggt tcatctccct ctcgatcccg gccggcgtcc tggtgtactg
     3781 ggtgaccacg aacatgtggc agatcggcca gcagtacgtg atgatcaaga agcgcgaggc
     3841 cggtgagggt caggccgccc ccgccaagcc cgccgcggcc aagggcgacc gcgaggaccc
     3901 cgagcccaag ccgcagcccc ggaagagcgg aggacgtcga gatggaagag gtcgaaagaa
     3961 acgccgcaag cgttgaggaa gcggtcgagg ccgccctcga ggagctcggc atcaccgaac
     4021 aggaggctca ggtcgaggtc ctccaggagc cccagaaggg cctcttgggg atcgggagca
     4081 agggcgcggt ggtcagggtc cgtcggcgca ccctgctggg gcaggccacc gaggagcagc
     4141 tccgggtggc catcgacttc ctcgccggcc tgatggagcg gctcggagtg aaggctgagg
     4201 tcgaggaaga gacgcacgag aacgtgcagt acctcgacgt cgtggcggcc ggcgatcctg
     4261 acgcgatggg cctcctgatc gggaggcacg gccagacgct cgacgcgatc caggagctga
     4321 ccagggcggc cgtgcagcgg cgaaccgccg agcgatgccg catcgtcgtg gatgtggagg
     4381 actaccggaa gcgacggcgc gcccagctgg tggagcgggc ccgcgaggct gcggagaagg
     4441 tccagcggtc cggtcgccgc cacacaatgg agccgatgac ccccttcgag cggaagatcg
     4501 tgcacgacgt cgtggcggag ttccccgggc tggagtcggg gagcgagggg caggagccga
     4561 agcggcgcgt ggtgatccgg aagcggcgat aggtttcacg tgaaacgctc ccgggtcggt
     4621 tccacgtgaa acgtctcgac gagtacgagg agctcctcag ggaccgggcc atcccgatgg
     4681 gactgatcgc cgcctccgac gcggagggcc tcagggagag gcacatcgac gactcgctcc
     4741 gcgcgacccg ggccttccgg gacgacgacg catgctgcat cgatgtgggc tcgggtgccg
     4801 gcctcccggg gatcccgctc gccatcgcgc tccccgatcg cgagttcgtg ctgatcgagt
     4861 cccgccgccg ccgggtcggg ttcttggagc tcgccatcga cctgctggag ctggcgaacg
     4921 tccgcgtgct ccctgatcga gtcgagtcgg tgggggagcg ggtccgggcc gggctgatgg
     4981 ccccggccga cctggccacc gcccgcgcct tcggccccct ggaacggagc tggcgctcgg
     5041 ccgctcccct cctcaggccc ggcggtcgcc tcgtgtactt cggtggccgg ggactggagg
     5101 accccgtggg gacggcgcgc tcggcggggg tggacgggga gatctcggtc cttgattcct
     5161 cgtcgccgct cgttatcatg gggcggcggt gagcctcgac gacgagcggc ctcacgcgga
     5221 gccttccccg ccggacgggg ggtccccgca cgccccctcc gacccgggcg tggagccccc
     5281 cgccgagccc ccggggccag agcgtgaggg cgagccggcg gaacgaccca cggagccggc
     5341 gagccccatc ggcccgtcag cagcttcccc gaaccctcaa gttcgagtcg agggttcgct
     5401 cgagcagcct cccgccgagg ccggcggcag gattatcgcg gtggccaacc agaaaggggg
     5461 cgtggggaag agcaccaccg ccgtgaacct gggcgcggcc ctggccgagt ccggggcccg
     5521 ggtcctggtc gtggacatgg atccgcaggg gaacgcgtcg accgggttgg ggatctcgcc
     5581 gcaggaccgc gagatcacca cctatcaggt cctggccgcg gggcttccgg cggcccgggc
     5641 catcatggcg acggcggtgg agggcctctc cgcgatcacc tcgaccatcg atctggcggg
     5701 ggccgagatc gagctcgtga gccagttctc gagggagatg cgcctggccc gggccttgga
     5761 cggggtggtt gaggggtacg actacgtgat cgtggactgc ccgccctcgc tgggcctcct
     5821 gacggtgaac tcgctggccg ctgccgacga gctgattgtc cccatccagt gcgagtacta
     5881 cgcgctggag gggctggggc agctgcttcg gaacgtgagg ctcgtccagc agaacctcaa
     5941 cccgagactg cgactgaccg ggatcgtgct gacgatgtac gactcgagga cgaagctggc
     6001 cgagcaggtc gtggctgagg tccggaagta cttcgggcgc cgtgtgtatg acacgctcat
     6061 acctcgaagc gtgcggctgt cggaggcgcc ggggtacggg cagccgatca cgacctacga
     6121 ccccgggtcg aagggggcgg aggcgtaccg ggcgctggcg tcggaggtgg cccagggccc
     6181 gcgggaagtc gagaccccca tgccgaccga ggacatgccg gaggtggttc tccccgagcc
     6241 cagcccggag gacctcgacg gcgaggacga gcaggagccc gagccggcgg tcaagaggag
     6301 gcgggggctc ttccggagga agggagacga gtcgtgaaac agcggggtgg actcggaagg
     6361 gggctctcgg ccctgatccc gggggcgacc gaggaaggtg ggctcctgga gatcccgctc
     6421 gccgcgatcg cccccaacac ccgtcagccg agggaggcgt tcgacgagga ggggatcgcg
     6481 gcgctggcca gctcgatcca ggaggtggga gtcctccagc ccatcgtggt gcggcggcgc
     6541 gaggacgggt acgagctggt ggcgggggag cggcgtctac gcgcggcgaa ggtggccggg
     6601 ctggccacga tccccgcggt gatcagggac accgacgaca ccgactcgct ccgcgaggcg
     6661 atcatcgaga acatccaccg ggaggacctc tcgcccctgg aacaggccgc cgcgttccag
     6721 gagctgctcg aggacctcgg tgcctcgcac gacgagctcg cggagcgcct ggggtactcg
     6781 cggtcccacg tcacgaacac gatccggctg ctccagctgc cgcccccggt ccagcgcttg
     6841 gtgggggagg ggcggctgtc ggccggccac gctcgcgccc tgctggccct ggacgacgtg
     6901 gaaggtcagg aggcgctcgc cctccgcgtg gcggcggagg ggctctccgt ccgccaggtg
     6961 gaggagctcg tccgcactta ccgcggaggg ggagagcggg gtgcggaacg ggcggcgccg
     7021 gagcccgacc cgcgggccgt gcaggtcgag gagctcctgg gggacgcgct cgccacccgg
     7081 gtcaggatcc agcgggggcg gcggaagggg aagatcgtca tcgagttcgg gtccgacgac
     7141 gacctggaca ggatcgtctc agcgatcgcg gggtcatgag cccggagggg cggcgagccg
     7201 cgagggccat cgccctcgga gcgctggtgg gggcactgat cgcgctgctg gcccgccgtc
     7261 ggggctgacg tgccccgggt cgtcgacgtg ggtctcgaga acctgtcctc ggcgccgccg
     7321 aaggtcctgg agtgcgcgtt ctgggagacc gagcgagagg accccgccga cccccgcttc
     7381 tacaaggagg agtggttctc ctccacgctg ctggagtggg ggagctgcgg gaagctgctg
     7441 gtcgacgagg acgaggtact ggccttcgcg cagttcgccc cgccgtcgct cttcccgcgc
     7501 gtcgccgggt accgagcggg tcgggtctcg cccgacgcgg tgtacctcgc ctactgctac
     7561 gtgacgacgg gccaccgcgg gcgagggctg ggctcccaca tgatcagggc ggtggcccgc
     7621 gacctcgtgg atcgcggctt ccgcgccgtg gagtcgctgg gccagtcgga atgggagggc
     7681 gggtgggtgc tccccgaagc cttcctcgcc gcgaatcgct tcgccgtggt ccggccggac
     7741 ccggcggtgc cgctgatgcg cctggagctc ttcggcgtgg cgacgcccgc cggggcccgc
     7801 gccgccgagg cgcttcccct gcctcgctag tcggagcccg ccagccccgc gacgtaccgc
     7861 tcgagggcct ccacgatcgc ctccgccaga tcgtggtgga accgtccctc gctcaggaga
     7921 cgctcttctc gagggttcga gatgaagccg ggctcgagat ggaccgccgg catcggggtt
     7981 tcacggagga gagggaaggc cttggggtgg gcccgtccgt cgcggaggcc gagtcgagac
     8041 gtgatccgct ccaggagcac ctcggccagg gcccgtcccg ccaccgagac ggcgccgagc
     8101 cgaccgaagt agtaggcggc cgccccctcg gcggtggggt cctcgtgatg gttgaggtgg
     8161 atcgagagga ggaggtcggc gccggaggcg ttcgctcggt cgatccgagc ggccaccgtg
     8221 ggatcctcgc cgggctcccg aagcagcacc gccttcgcgc cgcgcccccc gagcgcctcg
     8281 gccaggcgct ctgcgatggc ataggtgatc tcgctctcca cggaccctcc cgggcccacc
     8341 tcccccgggt cccccggacc gtgcgcgggg tcgatggcga ccgtcttccc ctcgagtgag
     8401 ccggccagcc ggaggtcctc ggtctctcgg accaacgtcc ggcccggtcc ggggaccgag
     8461 gcccggagcc gctccagcgc ctggaggctc gtgctcccca cgatgccgtc ggaggggagg
     8521 cccacgttcc tctggaagtc gcgcatcgcg acctcggtct ggcggccgaa gatcccgtcc
     8581 tcccggccgg ggtcgaatcc gagggtgttg agccgggctt ggagggcccg gacgtcgtcg
     8641 cctcggaagg gaggatgccg aaggtacagg acgcgatcgc cgagcgcgta gccggcctcg
     8701 acgagctcct cccaagcctg ggggcccacg agcccgtcca cgagcagccc tcgatcctgc
     8761 tggaacgccc ggacggccgc gtcggtggat ccctcgaacc gatcctcctc cagttcgctc
     8821 tggctgatcg tgtagcccag ggcgagcagg cgcgcc
//
LOCUS       DASUTW010000306         2978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1268777, whole genome shotgun sequence.
ACCESSION   DASUTW010000306 DASUTW010000000
VERSION     DASUTW010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268777"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VF058_10570"
     CDS             complement(<1..240)
                     /locus_tag="VF058_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease domain-containing protein"
                     /protein_id="HEX6208783.1"
                     /translation="MVEMTLVGVRVELPANQPIVLLRETEGQRYLPIWIGAAEATAIA
                     LAMQGVTPMRPMTHDLFKNVLDELHVALEKIEITEL"
     gene            complement(286..873)
                     /gene="hpt"
                     /locus_tag="VF058_10575"
     CDS             complement(286..873)
                     /gene="hpt"
                     /locus_tag="VF058_10575"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX6208784.1"
                     /translation="MLRAMEEGARVLYREDAIEQRVRELAAEISRDYAGRDPILVSVL
                     KGGAWFLADLIKATTINCRIDFMAISSYGGAEESMGVVRILQDLDLDIGGHHVIMVED
                     IVDTGLTLSYLLSTLSSREPASLAVCALLDKSVRRIAPVEIRYRGFDCPDRFVVGYGL
                     DHDERYRNLRCILEVEPEELRRDPLALVPYLDRRG"
     gene            complement(904..2127)
                     /locus_tag="VF058_10580"
     CDS             complement(904..2127)
                     /locus_tag="VF058_10580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX6208785.1"
                     /translation="MRRIVGATLAATLASTTPAAGQTGPPPTPVPPFGSPSPYPQSLE
                     TPAPSARPPRIEAPSAVLVDLTTGRVLWERRADHPRPIASLTKIMTVLIALESSDLDD
                     VVTVSDRATAEEGSELGLVAGERISVEDLLYALMLQSSNDGAIALAEHLGGSVDRFAR
                     MMNRRARELGLEDTSFTSPHGLDDRGRSTARDLAALTTEAFTHPAFVRIARTKVRAIP
                     AAEGPPRRIQSRNAMLWLYPGSVGVKTGFTSAAGFCLVAAAERDGRRFAAVVLGGPSA
                     AFSDAAAVLDHGFFTWRVRPVVEEGARLQPVSIDGRDVPVETGATIEGLVRRGQGAVL
                     RSLLDPSAGLPVRAGDVVGEVAAIVEGEEVGRAPLVAAADVGSRAQAPTLPPWWDDVL
                     DSIASTVPRWIEAVL"
     gene            complement(2141..2851)
                     /locus_tag="VF058_10585"
     CDS             complement(2141..2851)
                     /locus_tag="VF058_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX6208786.1"
                     /translation="MAGKEHLSIGEVLVSLKPEFPDVTISKIRFLESEGLIEPDRTES
                     GYRRFSESDVERLHEILRLQRDEYLPLKVIRDRLDRGPERNGPRPAVAGPRDEPSPED
                     EMALPATDVQLTPEELAQAGGVELSLIEELESFGLLSSRTVSGVRYYDGDSLVLLKIL
                     KDFFTYGVEPRHLRMYRHFADREAAFFQAIILPVLRQRNPEARKAASESLSDLARISR
                     QLKQALLRVNLREYLGNG"
     gene            complement(2851..>2978)
                     /locus_tag="VF058_10590"
     CDS             complement(2851..>2978)
                     /locus_tag="VF058_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX6208787.1"
                     /translation="IHDMGSLNGTYVNRDRVDSTQLASGDEVQIGKFKLVFFMGE"
BASE COUNT          449 a         1067 c         1044 g          418 t
ORIGIN      
        1 gagctcggtg atctcgatct tctccagggc cacgtgcagc tcgtccagga cgttcttgaa
       61 gaggtcgtgg gtcatgggcc gcatcggcgt gacgccctgc atcgccagcg cgatcgcggt
      121 ggcctccgcg gcgccgatcc atatcgggag gtagcgctgg ccctcggtct cacgcagcag
      181 cacgatcggc tggttggccg gcagctccac ccggaccccg acgagcgtca tctcgaccat
      241 gcccttcgac tgtatcctca gtcccgcaga ccggcaaccg ttccgtcagc cgcggcgatc
      301 gaggtagggc acgagagcca gcgggtcgcg ccggagctcc tcgggctcca cctcgaggat
      361 gcaccggagg ttgcggtacc gctcgtcgtg gtcgagaccg tagccgacga cgaaccggtc
      421 gggacagtcg aagccccggt agcggatctc caccggcgcg atccgccgca ccgacttgtc
      481 gagcagtgcg cagacggcga gcgaggcggg ctctcgcgag gacagcgtcg agagcaggta
      541 cgagagggtg aggcccgtgt ccacgatgtc ctcgaccatg atcacgtgat ggccaccgat
      601 gtcgaggtcc aggtcctgga ggatccggac gacgcccatg ctctcctcgg ccccgccgta
      661 cgaggagatg gccatgaagt cgatgcggca gttgatcgtg gtggccttga tgagatcggc
      721 caggaaccag gcgccgccct tgagcaccga gacgaggatg gggtcccggc ccgcgtagtc
      781 gcggctgatc tcggcggcca gctctcggac ccgctgctcg atcgcgtcct cccggtagag
      841 gacgcgcgca ccctcctcca tcgcccgtag catacccgcg cctccggccc gagccgtgga
      901 gcctcagagg acggcctcga tccaccgcgg gacggtcgag gcgatcgagt cgaggacgtc
      961 gtcccaccac ggcgggaggg tgggggcctg ggcccggctc ccgacgtcgg cggcggccac
     1021 gagcggggcc cggccgacct cctccccctc gacgatcgca gccacctcgc cgacgacgtc
     1081 gccggcccgg acgggcaggc cggccgaggg atcgagcagg gaccgaagca cggccccctg
     1141 ccccctgcgc accagcccct cgatcgtggc cccggtctcg acggggacgt cgcggccgtc
     1201 gatcgacacc ggctgcagcc gcgcgccctc ctcgaccacc gggcgcaccc gccaggtgaa
     1261 gaagccgtga tccaggaccg ctgcggcgtc ggagaaggcg gccgagggcc cgcccaggac
     1321 gacggcggcg aagcgccggc cgtcccgctc ggccgcggcc acgaggcaga agccggccgc
     1381 tgaggtgaag cccgtcttga cgccgacgct ccccggatac agccagagca tcgcgttccg
     1441 gctctggatc cggcggggcg ggccctcggc cgccgggatg gcccggacct tggtgcgcgc
     1501 gatccgcacg aacgcgggat gggtgaaggc ctcggtggtg agggcggcca ggtcccgggc
     1561 cgtggaccgt ccgcggtcgt ccaggccgtg ggggctcgtg aacgacgtgt cctcgaggcc
     1621 gagctcgcgg gcccgacggt tcatcatccg agcgaaacga tccaccgacc ctccgaggtg
     1681 ctcggcgagc gcaatcgccc cgtcgttcga ggactgcagc atcagcgcgt agaggaggtc
     1741 ctccaccgag atccgctccc ccgccacgag ccccagctcg gacccttcct cggccgtcgc
     1801 gcgatcgctg acggtgacca cgtcgtcgag gtccgaagac tccagcgcga tgagcaccgt
     1861 catgatcttc gtcaggctgg cgatggggcg cgggtggtcc gcccgccgtt cccacagcac
     1921 ccggccggtc gtgaggtcga cgaggacggc cgacggagcc tcgatgcggg gcggccgggc
     1981 agacggggcc ggcgtctcca gcgactgagg gtacggggag ggcgacccga acggagggac
     2041 cggggtcggg ggcggccccg tctgcccggc cgccggcgtg gtcgaggcga gggtcgccgc
     2101 cagggtcgct ccgacgatcc gtcgcatccg aggggcgcgt tcagccgttc ccgagatact
     2161 cgcgcaggtt cacgcgcaga agggcctgct tgagctgccg cgagatccgc gccaggtcgc
     2221 tcagcgactc cgacgccgcc ttccgcgcct cagggttccg ctgacgaagg acggggagga
     2281 tgatcgcctg gaagaaggcc gcctctcggt cggcgaagtg ccggtacatc cgaaggtgcc
     2341 tgggctccac gccgtaggtg aagaagtcct tgaggatctt gagcagcacc agactgtccc
     2401 cgtcgtagta gcgcaccccg ctcaccgtcc gcgacgaaag gagcccgaac gactccagct
     2461 cctcgatcag cgacagctcc accccgccgg cctgcgccag ctcctcgggc gtgagctgga
     2521 cgtcggtggc cggcagcgcc atctcatcct cgggcgacgg ctcgtcgcga ggcccggcca
     2581 ccgccggccg cggcccgttc cgctcggggc cccggtccag ccgatcccgg atgaccttga
     2641 gcgggaggta ctcgtcgcgc tggagccgca ggatctcgtg gagccgctcc acgtccgact
     2701 ccgagaacct ccgatagccc gactcggtgc ggtccggctc gatcaggccc tcgctctcca
     2761 ggaaccggat cttggagatc gtgacgtcgg ggaactcggg cttcagcgag acgaggacct
     2821 ccccgatgga caggtgctcc ttccccgcca tcactccccc atgaagaaca cgagcttgaa
     2881 cttgccgatc tgcacctcgt ccccgctggc gagctgcgtg ctgtccaccc ggtcgcggtt
     2941 gacgtacgtg ccgttgaggc tccccatgtc gtggatga
//
LOCUS       DASUTW010000307        15922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1658536, whole genome shotgun sequence.
ACCESSION   DASUTW010000307 DASUTW010000000
VERSION     DASUTW010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15922
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1658536"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VF058_10595"
     CDS             <1..547
                     /locus_tag="VF058_10595"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6208788.1"
                     /translation="SELPGITGSFLRVHFGIILALLAAWGVWWLLFRTTKGFEFRAVG
                     SNPNAARYAGMSVGLTYVLAMSLAGSLAGLGGASQVLGLQKFLTPGFASGIGFTAIAL
                     ALLGKAHPLGVVAASLLFGMLQAGAVSMQAATRTPVDIIVVIQALIIVFIAAPSLVRA
                     IYRISMGREVGAEVFTKGWSG"
     gene            544..1794
                     /locus_tag="VF058_10600"
     CDS             544..1794
                     /locus_tag="VF058_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6208789.1"
                     /translation="MSATAATVSGRISVRRARVLGILFLLLAAFTAGFFGPRAPGGET
                     ASFALGANAPDLSLPVQATLYALSALMAFLGAVQLVRGFGPRTYVILGIVLGVFIFSL
                     LVYAARGATLNLTAMLNSTLLRSVPITLGALSGVLCERSGVINIGIEGMFLAAAFTGA
                     IVGSASGSLWAGLISGVLVGGLLGWVLAVLSIRYRVDQIVAGTVINIFALGITSFLSA
                     SILQENQALNNPGRFQVFEIPLLSEIPVAGPVLFRNNAFVYLMFGLLFLIHIALFRTR
                     WGLRVRAVGEHPLAADTVGIRVLFERYRSVIFGGMVAGLGGAFFTLGSVGRFDEGMTA
                     GRGFIALAAMIFGRWSPWGAFGAALVFGFADSLQTRLAILEVGLPSEFLLMAPYIATI
                     LVVAGLVGRARPPAADGKPYIKEQ"
     gene            1982..3031
                     /gene="aroF"
                     /locus_tag="VF058_10605"
     CDS             1982..3031
                     /gene="aroF"
                     /locus_tag="VF058_10605"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017617063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX6208790.1"
                     /translation="MVVVMKMGASDAEVDGVVEKVTESGGEAFVSRGRFWTIIGLVGD
                     TARFLELPLESLPGVDRVVRVGRPYKMVARELHPEPSTVRIGGVAIGRQSVTVIAGPC
                     AIESEEQALASAEMARRAGAHLLRGGAYKPRTSPYAFQGLRERGLEILAACREATGLP
                     VVTEVLEARDVERVAEVADGLQIGARNMHHFPLLQEVGQTGKPIVLKRGLSATVEEWL
                     MAAEYVAQQGNSDIVLCERGIRTFEPSTRNTLDLAGMVVAREASHLPVIADPSHATGQ
                     RGLVTPMAKAAVAAGADGLMVEVHPRPDEALCDGPQALTGGDLDALVAEVSALARSMG
                     RFVAEAPASVGESGT"
     gene            3028..4032
                     /gene="trpS"
                     /locus_tag="VF058_10610"
     CDS             3028..4032
                     /gene="trpS"
                     /locus_tag="VF058_10610"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX6208791.1"
                     /translation="MTEPKRVLSGIQPTGEIHIGNLIGAVRQWVADQDEFDTFYVIVD
                     LHAITVPQEPGALREATVRMAASLIAAGLDPERCTLFVQSHVHEHAELAWVLGTLAGF
                     GELRRMTQFKDKAARQQEASVSVGLFTYPVLQAADILMYQADRVPVGADQRQHIELTR
                     DIAGRFNTRYGETFVLPEAAIPRVGARVMDLQDASTKMSASASSQQGVLRLADDPEVL
                     RKKIRSAVTDSGREIVAREDKPEISNLLTIMSVATGRDVSSLEAEYGGKGYGDFKGDL
                     AEAVIEFLRPIQERYAALRGDPAELARQLEIGAGKAQAVAAKTLQTVYERVGFLPRGG
                     "
     gene            complement(4029..5372)
                     /locus_tag="VF058_10615"
     CDS             complement(4029..5372)
                     /locus_tag="VF058_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6208792.1"
                     /translation="MYRTATATVHALRGVTIEFPRSRMTAVAGPSGSGKSSLLRLLAG
                     LDRPTSGILEVDGVQLHRATGRARRAFRRRRVGYVFQRPSDNFIPHLTVGQHLAMAAR
                     SAGEGAPSMKEVAEELGIGGRLDHLPSALSGGEQERAAVAQALVRGTDIIVADEPTAD
                     LDTASTETLLARLSALASEGLTIILATHDASVRRAADHVVELDHGSLVPASRRRTPAP
                     AGRASARGTTAPLLVTATGLVRSFARGPETVHAVDHADLTLREGEVVALLGRSGSGKT
                     TLLDLLGGWERPDAGAITWHGDVAVREPAWEDVSIVPQQLGLIEEFSLRRNVEYPLRL
                     AGTPDADRRVDGLFEKLRLSHLGDRFPGEVSVGEQQRAALARALARRPRLLLADEPTG
                     HQDRESARAVAQLLGEAASEGTCCLVATHEQHLSAFADRVVRMADGRVSEDGSRP"
     gene            complement(5404..8097)
                     /locus_tag="VF058_10620"
     CDS             complement(5404..8097)
                     /locus_tag="VF058_10620"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX6208793.1"
                     /translation="MGLRANPLWTTAPSALLHSPGLWTAVAAGTLLLAVAATFAPAFL
                     SATERELLAREIDDPSVTRYGAGISFEISSLPLDRTVRGRPLHVGQRRVFSGRVSEDR
                     RLAAVVEGILSPEVETRDGSRSRSAKLMFRTDAARHVRILEGEAEGVLIPELLARGLL
                     VGPGDSIEVRHQGGPPVSLTVGGVYETLYTTPRTGYWRSWAEEIWPCLGCDPPPQPIL
                     VDRAQLLDAMSTLGVESAAFAWDAPLRSIGALTASDAEGVARTFSAFLAEATGVPGAR
                     SEEAEILLCCGFRFEPFRTRTALTSAMPAVLTIVERRMIALEGPVRVLSLAGVLVAVV
                     TVAAAGAFATAARRREYTLLYATGLRTSSVALKGFLESVAPAAVGAAAGLGLSLLLVA
                     WLAPEGPMSGPTLRSTIFGAAIVWAAAASLVGAVTAALFRRHAEVAHRTGMGLPFDVA
                     LILVGIYLLDRVTGAGALVVDPETGIETPSLALLALPVAGLAGIGGLAARAFHLGLSR
                     AREGLRMRSEALYLAVHRLAGARRLALLLVAAGAASIGLLLHALTVARSLDATVHAKA
                     RVFVGSEVAAQIDPEFVPTREYGLPHTVVTRWRSGGRFAEVGRQFDVLAVDPDTVPGA
                     AFWDDSFSHAPLEELMARLEASGDGTLPVIVAGLGDDLPERTTLILGRTEVSARIVAT
                     ARAFPGMSSGDPLVVAGEEALRGDGPSGPLVGGRATGAVWVRATPRVAARRLIASGIH
                     PDDILTARQVEDLPSIEAAAGTFAILAGLGLVSGLLVLVVLVMYVQARQRAQVVSYAL
                     ARRMGLRRGANVLALVLEVGTMLLTSFLVGAVVGVGISFFVVPEIDPLPAIATEPLMR
                     SPVTASILTLTALAACTAMASWLAERAARRTNVAEVMRVAE"
     gene            complement(8174..8956)
                     /locus_tag="VF058_10625"
     CDS             complement(8174..8956)
                     /locus_tag="VF058_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208794.1"
                     /translation="MPKAELVFGNVLDGRVHEADGVEGPSFVYGFSAPGRALPFAVIR
                     HWKAPQGVVVEGFEIISPNGTTAYRSTTRTRRMPGQMDLTEIVDVVDDAVLPELGVHV
                     VSFLIDGEVEGQVEFQYVLQQPPDKLPKELEDGFRKTDAIWVGVDRDGRDETIPVWFA
                     YHQGRVYLLHAIKDDSPEQRVPGADDATDLLVITRHKYRDTRLSRLHATARVIEPSDG
                     DFARYAGILADRRRDRHGPPEDAIASWRQGHVILELTPLVPG"
     gene            complement(8994..10175)
                     /locus_tag="VF058_10630"
     CDS             complement(8994..10175)
                     /locus_tag="VF058_10630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX6208795.1"
                     /translation="MADEPFSDDVFKDVPLFRELQRVLFSGSGPVNWELARQVGIATA
                     VGDRPDPEPTDEDRAMLEPVVRAAELAVADLTGLEAPPEVSRIRPVRRAKWVEASVTG
                     LRGLFEPGAEKLGKMMLEAPGHELGGEGAPGLEGLAGLGALMGRMGPLLMGTQVGMVL
                     GMLGRRALGQYDLAVPREGQDALLFVVPALEELEREWDLPRDDVRAWVAVHEVTHAFQ
                     LGRGWVREHFLEQIRELAAGMEFDLSGLEERLAGVDLSDPERLQEAIGDPADLLGQKL
                     TDEQRLLMRRVQALMAAAEAHADHVLEVLGERMLTQAARIEEAMLRRREDRSEHERMA
                     ERLLGFELTDEHLRLGRAFVERVARATDERALASMWESAESLPSMPELEEPTLWLSRM
                     A"
     gene            10203..11057
                     /locus_tag="VF058_10635"
     CDS             10203..11057
                     /locus_tag="VF058_10635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6208796.1"
                     /translation="MRIRPAEESDLRALAEIQVAGREWLARRYEPETLHQFPVDPDGA
                     AFTIRHVHGTGAVFVAEDPEPVGFSSAVIRDGVWFLSQLWVAPDRHGAGIGSALLDAA
                     LAWGRGSSTFSVVASPFPGAQTVYMRRSMFPVWHSMEFEGEAPGVEMPEGADELTTGD
                     QPWVDELDREVRGIARPEDHELFREHARGIALRRGDEPLGYLYAWPEGKLGPAAIRDG
                     RDTPDLLRAGIAAGPSRLSLIVPTTNWAALQEAIRLRLRLVSRTVFMASRAMPDGSRY
                     LSSGGALA"
     gene            11096..12091
                     /locus_tag="VF058_10640"
     CDS             11096..12091
                     /locus_tag="VF058_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX6208797.1"
                     /translation="MRAGDLAIDLGTANTLVFSQERGIILNQPTVLAVNGRDGSVLAV
                     GEEAWEMIGRTPANIVAVRPMRSGAITDYDLTAQMMRLVLRRVGATRFHKPRVLLCVP
                     SAITEVERRAVSEASISAGARSVQLIEEPMAGAIGAGLPVHEPLGNLLVDVGGGTTEV
                     AMISMGGIVEARSTRVGGFDLDDAVQHYLRQRYGLAVGERAAETVKIAIGTAYPAGVP
                     PYSIRGREITSGMPKEVQLTQEEIREAISDLVAHIVDAARDTLASSPPELGHDVLERG
                     MFLTGGGGLLRGLDMRIAQECEVPVHLTDSPLETVVLGAGKCLDLPADVPGLFAQ"
     gene            12103..12939
                     /locus_tag="VF058_10645"
     CDS             12103..12939
                     /locus_tag="VF058_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX6208798.1"
                     /translation="MAADLEAGDWGVAVASKFPAVGAVVPWARAGVGAVATQAWANTS
                     FGPRGLDLMAGGAEANEALRQMLDEDEGRVQRQVGMVDSHGIVATFTGEGCMEWAGGH
                     TGEGFACQGNILVGPEVVEAMAEAYEATEGDLLDRLLAAVAAGDAAGGDRRGRQSAAI
                     LVVREGGGYGGYTDRYVDLRIDDHPDAVTELARLVDVYDREILVRDDPLLEATPELVS
                     DLQRRLGRLGRYRGEAHGAYDEDTRAAIEGFAGEYNLEGRLRDDEMISGSLVDEIRDV
                     TP"
     gene            complement(12936..13220)
                     /locus_tag="VF058_10650"
     CDS             complement(12936..13220)
                     /locus_tag="VF058_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208799.1"
                     /translation="MELFLLVMTAAVVGWLLWQLTEGSHDHRWTGVIEPPAPDAGLRS
                     LRSTPDERIHPVVAATLLVLSILIVALGVAAGVYFAGRFAIERLGQMLGL"
     gene            13278..14243
                     /locus_tag="VF058_10655"
     CDS             13278..14243
                     /locus_tag="VF058_10655"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEX6208800.1"
                     /translation="MRRLLLSALPLVSLATALSLVDIGMFTIGPGSAEEVFPRIQLDG
                     VGVTEPSGRLLLTTVSIPRATVFHAVWAGWDPAVELVPEEAILGQRTEREHERLTRSQ
                     MDESKIAAVSIAATDLTDYPEEHGPGALVQNVLGGTAAAGKLLPGDLIVSVDDDPVDD
                     VDDVSRAIVATEARRALALTVEAGGEERAVRIRPRMSEEEGRPIIGVILIEPFPFEVE
                     IESGRIGGPSAGLMWTLGVYDLLSEEDLVDGRRIAGTGAIDLAGNVYAVGGAAQKVRA
                     ARQAGAEVFLVPRENLEEARSVDAGIRVVPVRNVRAALRFLRGTS"
     gene            14270..>15922
                     /locus_tag="VF058_10660"
     CDS             14270..>15922
                     /locus_tag="VF058_10660"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX6208801.1"
                     /translation="MDRPPPPRRAAPRRRWLLWVGGAVLAIVVVFGALSGFYVDILWF
                     REVEQSSVFWTIFWSRVLLATVFGALFFALLYANLLIARAVRPRYQVFAPGEEAVDRY
                     RTLIEPYARWALLGVSVLLALFAAAGVAAQWEAYQQWRVAGSVSFGATDPVFERDVAF
                     HVLSLPFQRFVQSWLFSSLVAVTVVTAAAHYLWGGIRFRAVAERVTPQVKAHLSVLLG
                     LIVLVRAWGYRLGQFDLLLSPRGTVTGASYTDLNAQLPALQLLVVISIIVTVLFLVNI
                     RFRGWALPAIGLGLLVLTSVLAGAVWPAAIQRFRVDPQELQREREFITRNIDFTRQAY
                     GLDGVEAESFPAEPELTREELEENIGTVEDIRLWNPSTLARAYFQLQRIRPYYEFVDV
                     DVDRYEIDGEKVLTMLAPREIAQDTIPGGQTWQNRHLVYTHGFGAAASRADQLDVAGT
                     PRFLLRDVPPAGDLAASLEQPRVYFGEETDVPFSLVRTEQQELDFPVGENEFARTTYD
                     GEGGIEVGGFFRRLAFAWRYRDVNLLISGLVRDDTKILINTDL"
BASE COUNT         2176 a         5600 c         5866 g         2280 t
ORIGIN      
        1 atcggagctg ccgggcatca cgggctcctt cctccgcgtc cacttcggca tcatcctggc
       61 cctgctggcg gcctggggcg tctggtggct gctcttccgg accaccaagg gcttcgagtt
      121 ccgagccgtc ggctcgaacc cgaacgcggc ccggtacgcg gggatgagcg tggggctgac
      181 ctacgtcctg gcgatgagcc tcgccgggtc cctggccggg ctcgggggcg cgtcgcaggt
      241 gctcgggctc cagaagttcc tgacgcccgg cttcgccagc ggcatcgggt tcacggccat
      301 cgcgctggcg ctgctcggca aggcacaccc gctgggcgtc gtcgcggcgt cgctgctgtt
      361 cgggatgctc caggccggcg cggtctcgat gcaggccgcc acccggacgc cggtggacat
      421 catcgtggtg atccaggccc tgatcatcgt gttcatcgcg gcgccctcgc tcgtccgggc
      481 gatctaccgg atcagcatgg gccgtgaggt gggggcggag gtcttcacca aggggtggag
      541 cggatgagcg ccacggccgc caccgtctcc ggccgcatct ccgtccgcag ggcgcgagtg
      601 ctggggatcc tgttcctgct gctggccgcc tttacggccg ggtttttcgg ccccagggcc
      661 cccgggggag agaccgcatc cttcgccctg ggggcgaacg ccccggacct ctccctcccg
      721 gtccaggcga ccctgtacgc gctctcggcc ctgatggctt tcctgggggc cgtccagctc
      781 gtgcgcggct tcggccctcg gacctacgtg atcctgggaa tcgtccttgg ggtcttcatc
      841 ttctccctcc tcgtgtacgc ggcccgcggc gccaccctga atctcaccgc gatgctcaac
      901 tccacgctcc tccgctcggt ccccatcacc ctcggggcgc tgtcgggcgt cctgtgcgag
      961 cggtccggcg tgatcaacat cgggatcgag gggatgttcc tggcggcggc gttcaccggt
     1021 gccatcgtgg ggagcgcctc ggggagcctc tgggccgggc tgatctcggg cgtcctcgtc
     1081 ggggggctcc tgggttgggt gctggccgtc ctctcgatcc gctaccgcgt ggaccagatc
     1141 gtcgcgggga ccgtcatcaa catcttcgcc ttggggatca cgtcgttcct ctcggcgagc
     1201 atcctgcagg agaaccaggc gctgaacaac ccgggccggt tccaggtgtt cgagatcccg
     1261 ctcctctcgg agatccccgt ggccgggccg gtcctgttcc ggaacaacgc cttcgtgtac
     1321 ctgatgttcg ggctgctgtt cctgatccac atcgcgttgt tccgcacccg gtggggcctc
     1381 cgggtgaggg cggtcgggga gcatccgctc gccgccgaca cggtcggcat ccgcgtgctc
     1441 ttcgagcggt atcggagcgt gatcttcggc gggatggtgg cgggcctcgg cggcgcgttc
     1501 ttcacgctag gctccgtggg acgcttcgac gaggggatga cggcgggccg gggattcatc
     1561 gccctggccg cgatgatctt cggtcgctgg tcgccgtggg gggccttcgg ggcggcgctg
     1621 gtgttcgggt tcgccgactc cctccaaacg cgcctggcga tcctggaggt ggggctcccc
     1681 tccgagttcc tcctgatggc gccgtacatc gccacgatcc tggtggtggc ggggctcgtg
     1741 ggccgggcca ggccgccggc ggcggacggc aagccctaca tcaaggagca gtaagcgggt
     1801 tgtgcgatcc gggccctccc tccacaatga gggcgacatg aagaacggtc ggcacggctt
     1861 cgcgtggtat cggagcggcg gagggcgctg ctcggactga cctgcggagc gaccggcaac
     1921 caggaccccg ggcagcggcc cggggttccg tgcttcccgg gccctcccac ggaggagcgc
     1981 catggtcgtc gtgatgaaga tgggggcgag cgacgccgag gtcgacggcg tcgtggagaa
     2041 ggtgaccgag tccggtggcg aggcgttcgt ctcgcgcggc cgcttctgga ccatcatcgg
     2101 gctggtcgga gacaccgcgc ggttcctcga gcttccgctc gagtccctgc ccggcgtgga
     2161 ccgggtggtg cgggtgggcc gcccgtacaa gatggtggcc cgggagctgc atcccgaacc
     2221 gtccaccgtg cgcatcgggg gcgtggcgat cggaaggcag tcggtgaccg tgatcgcggg
     2281 tccctgcgcg atcgagtccg aggagcaggc cctcgcctcc gcggagatgg ccaggcgggc
     2341 cggcgcccat ctgctccggg ggggcgccta caagccccgg acgtccccct acgcgttcca
     2401 ggggctccgc gaacgggggc tggagatcct cgcggcgtgt cgcgaggcca ccggcctccc
     2461 cgtcgtcacc gaggtgctgg aggcccgcga cgtggagagg gtggcggagg tggccgacgg
     2521 cctccagatc ggcgcccgga acatgcacca cttcccgctg ctgcaggagg tgggacagac
     2581 ggggaagccg atcgtcctga agcgcggact gtcggcgacc gtggaggagt ggctgatggc
     2641 cgcggagtac gtcgcccagc aggggaacag cgacatcgtc ctgtgcgagc gggggatccg
     2701 gaccttcgag ccgtccaccc ggaacacgct cgacctcgcc gggatggtgg tggcccggga
     2761 ggcctcccac ctcccggtga tcgccgaccc ctcgcacgcc accggccagc gcggtctcgt
     2821 cacgccgatg gccaaggccg cggtggcggc gggggccgac gggctgatgg tcgaggtcca
     2881 cccccgcccc gacgaggcgc tctgcgacgg gccccaggcg ctgacgggcg gcgacctcga
     2941 cgcgctcgtg gccgaggtgt cggcgctggc ccggtcgatg ggtcggttcg ttgcggaggc
     3001 cccggccagc gtgggagagt ccggcacatg accgagccga agcgcgtcct ctccggcatc
     3061 cagcccaccg gcgagatcca catcgggaac ctgatcggcg cggtccgcca gtgggtggcc
     3121 gatcaggacg agttcgacac tttctacgtg atcgtggatc tgcacgcgat cacggtcccg
     3181 caggagcccg gggccctccg ggaggccacg gtgaggatgg ccgccagcct gatcgcggcg
     3241 ggcctggacc cggagcgctg cacactcttc gtccagtcgc acgtgcacga gcacgcggag
     3301 ctggcgtggg tgctggggac gctcgcgggg ttcggcgagc tgcgccgcat gacgcagttc
     3361 aaagacaagg ccgcgcgcca gcaggaggcc agcgtcagcg tcggcctctt cacctacccc
     3421 gtgctccagg ctgcggacat cctgatgtac caggccgacc gcgtccccgt gggagcggac
     3481 cagcgtcagc acatcgagct gacgcgggac atcgcggggc ggttcaacac gcggtacggg
     3541 gagacgttcg tgctccccga ggccgccatc cccagggtgg gggccagggt catggacctc
     3601 caggatgcct cgacgaagat gtccgcgtcc gcctcgtcgc agcagggggt cctgcggctg
     3661 gcggacgatc cggaggtgct ccgcaagaag atccgctcgg cggtgaccga ctcgggccgg
     3721 gagatcgtgg cccgcgagga caagccggag atctcgaacc tgctcacgat catgtcggtg
     3781 gccaccggca gggacgtctc gtcgctggag gccgagtacg gggggaaggg ctacggcgac
     3841 ttcaagggag acctggccga ggccgtgatc gagttcctgc gtccgatcca ggagcggtac
     3901 gccgccctgc ggggcgaccc ggccgagctg gcccggcagc tggagatcgg cgcgggcaag
     3961 gcccaggcgg tcgccgccaa gacgctccag acggtctacg agcgggtcgg cttcctgcct
     4021 cgtggcggct agggacggct cccgtcttcg gacactcgtc cgtccgccat cctgaccacg
     4081 cggtccgcga acgcgctgag atgctgctcg tgggtggcca cgaggcagca ggtcccctcg
     4141 gaggcggcct cgcccagaag ctgggccacc gcccgggccg actcgcggtc ctggtgcccc
     4201 gtcggctcgt cggcgaggag gagacggggc cgcctggcca gggcccgggc cagggccgcc
     4261 cgctgctgct ccccgaccga gacctccccc gggaagcggt ccccgaggtg ggagaggcgc
     4321 agcttctcga acagaccgtc aaccctccga tcggcgtccg gggtgcccgc cagccgcagc
     4381 gggtactcga cgttcctgcg gagggagaac tcctcgatga ggccgagctg ctgggggacg
     4441 atggagacgt cctcccaggc cggctcgcgg accgccacgt ccccgtgcca cgtgatcgcc
     4501 ccggcgtcgg gccgctccca cccgcccagg aggtcgagga gggtcgtctt gccggacccc
     4561 gatcgtccca gcagcgccac cacctctcct tcacgcagcg tcaggtcggc gtggtcgacg
     4621 gcgtgaacgg tctccgggcc gcgcgcgaac gagcggacca gcccggtcgc ggtgacgagg
     4681 agcggggccg tcgtcccccg ggcgctcgcc cggccggccg gtgcaggagt gcggcggcga
     4741 gacgccggga ccagcgagcc gtgatcgagc tcgaccacgt ggtcggccgc ccgacgaacg
     4801 ctggcgtcgt gcgtggcgag gatgatcgtc aggccctcgg aagccagggc cgagagccgc
     4861 gcgagcagcg tctcggtgga tgcggtgtcg agatccgcgg tgggctcgtc cgcgacgatg
     4921 atgtccgtcc cccggaccag ggcctgcgca acggccgctc gctcctgctc tccccccgag
     4981 agcgcggagg gcaggtggtc caggcgacct ccgatgccca gctcctcggc gacctccttc
     5041 atggagggcg ctccctctcc cgcggatcgg gccgccatcg cgaggtgctg tcccacggtc
     5101 aggtggggga tgaagttgtc ggagggtcgc tggaagacgt accccacccg tcgtcggcgg
     5161 aacgctcgcc gggcgcgccc cgtcgccctg tggagttgga cgccgtccac ctcgaggatc
     5221 ccggacgtcg ggcgatccag cccggccagc agccggagga gggacgactt cccgctgccc
     5281 gacggcccgg ccaccgccgt catcctcgag cgtgggaact cgatcgtgac gcctctgagg
     5341 gcgtgcaccg tggccgtcgc cgtccggtac accttttcga gctcgcgaca ccgcgccgcc
     5401 cactcactcg gcaacgcgca tcacctccgc cacgttcgtg cgacgggccg cccgctccgc
     5461 cagccacgag gccatcgccg tgcaggccgc cagggccgtc agggtcagga tcgaggcggt
     5521 cacgggcgac ctcatgaggg gctcagtggc gatcgcgggg agggggtcga tctcgggcac
     5581 gacgaagaac gagatgccca cgcccaccac ggcgcccacc aggaaggagg tcagcagcat
     5641 cgtccccacc tcgagcacga gggccaggac gttcgcgccg cggcgaagcc ccatccggcg
     5701 cgccagcgca taggaaacga cttgagcccg ctgccgcgcc tgcacgtaca tcaccaggac
     5761 cacgaggacg aggagaccgc tcaccagccc cagccccgcc aggatggcga aggtgccggc
     5821 ggccgcctcg atcgagggca ggtcctcgac ctgccgggcg gtgaggatgt cgtcggggtg
     5881 gatgcccgac gcgatgagcc ggcgcgccgc gacccgaggg gtggcccgca cccagacggc
     5941 cccggtcgcc ctcccgccga ccagcgggcc cgaggggccg tcgccccgca gggcctcctc
     6001 gcccgcgacc acgaggggat cgcccgagct catgccgggg aacgcccgag cggtggcgac
     6061 gatccgggcg gagacctcgg ttctgccgag gatcagcgtg gtgcgctcag ggagatcgtc
     6121 gccgaggccg gcaacgatca ccggaagcgt tccgtcgccg gaggcctcca gccgggccat
     6181 cagctcctcg aggggcgcgt gggagaacga gtcgtcccag aaggccgccc cgggcaccgt
     6241 gtcggggtcc acggcgagga cgtcgaactg ccgccccacc tccgcgaacc gccctcccga
     6301 ccgccagcgg gtcaccacgg tgtgggggag gccgtactcg cgggttggga cgaactcggg
     6361 gtcgatctgg gccgccacct cgctcccgac gaagacgcgc gccttcgcgt ggacggttgc
     6421 gtccagcgac cgggccacgg tcagggcgtg gagcagcagc ccgatgctgg ccgcgcccgc
     6481 cgccaccagg agcagggcga gccgccgtgc cccggccagg cggtgaacgg cgaggtagag
     6541 ggcctccgac cgcatccgca gcccctctcg ggcccgtgag aggccgaggt ggaaggcgcg
     6601 agcggccagt cctccgatgc cggccagccc agcgaccggg agcgcgagca gtgccaggct
     6661 gggggtctcg atcccggtct ccggatcgac gaccaacgcc cccgcgccgg tgacccggtc
     6721 gaggaggtag atcccgacca ggatgagggc cacgtcgaac ggcagcccca tcccggtccg
     6781 gtgggcgacc tccgcgtgcc gtcggaagag cgccgccgtc accgcgccca ccaacgaggc
     6841 cgccgccgcc cagacgatcg cggcgccgaa gatcgtcgat cggagggtgg gccccgacat
     6901 cgggccctcg ggcgccagcc aggccacgag cagaaggctc agtccgaggc cggccgcggc
     6961 gccgacggcg gcgggcgcca cggactccag gaagcccttc agcgccacgc tgctcgtccg
     7021 gaggccggtg gcatacagga gcgtgtactc gcgccgccgg gccgcggtgg cgaaggcccc
     7081 cgccgcggcc accgtgacca cggcgaccag cacgccggcc aggctgagca cgcggacggg
     7141 accctccagc gcgatcatcc ggcgctccac gatggtcagg accgccggca tcgcggaggt
     7201 gagggctgtc ctcgttcgga acggctcgaa ccggaacccg cagcagagga ggatctccgc
     7261 ttcctccgag cgagcgccgg gcaccccggt ggcctcggcc aggaacgcgg agaaggtgcg
     7321 agccacccct tccgcgtcgg aggctgtgag cgccccgatc gaccggagag gggcgtccca
     7381 cgcgaaggcc gccgactcca cgcccagcgt cgacatcgcg tccagcagct gggctcggtc
     7441 cacgaggatc ggctggggcg gagggtcgca gccgaggcac ggccagatct cctcggccca
     7501 ggaccgccag tagcccgtcc ggggcgtcgt gtacagcgtc tcgtacacgc cccccacggt
     7561 caggctgacg ggggggccgc cctgatgacg gacctcgatc gagtcgccgg ggccgacgag
     7621 caggccccgc gccagcagct ccgggatgag gacgccctcc gcttcgccct ccaggatccg
     7681 gacgtggcgg gcggcgtccg tccggaacat cagcttggcg gagcgggacc tcgatccgtc
     7741 ccgggtctcg acctcggggc tgaggatgcc ctcgaccacg gccgccaggc gccggtcctc
     7801 cgacacccgt cccgagaaga cccgccgctg cccgacgtgg aggggccgac cgcgcacggt
     7861 gcggtccagg ggcaggctgc tgatctcgaa cgagatcccc gcgccgtagc gggtcacgga
     7921 cggatcgtcg atctccctgg ccaggagctc ccgctcggtg gcggagagga acgcgggggc
     7981 gaacgtggct gcgacggcca ggaggagcgt gcccgccgcg acggcggtcc acaggcccgg
     8041 actgtggagc agggccgagg gagcggtggt ccagagcggg ttggctcgca ggcccatcct
     8101 gcggctcatc ggctcatccc ggtcggcacc attggttcta cgtcctcccg gccgggccgg
     8161 atgtcctccc ggctcagccg ggcacgagcg gggtgagctc gaggatcacg tggccctgcc
     8221 gccacgacgc gatcgcgtcc tccggcggcc cgtgacggtc tcgccgacgg tcggccagga
     8281 tccccgcgta tcgagcgaag tccccgtcgc tcggctcgat caccctcgcc gtggcgtgga
     8341 gcctgctcag cctcgtgtcg cggtacttgt gccgcgtgat cacgaggaga tcggtggcgt
     8401 cgtcggcccc gggcacccgc tgctccgggg agtcgtcctt gatcgcgtgg aggaggtaga
     8461 cacggccctg gtgataggcg aaccagacgg ggatcgtctc gtccctcccg tcccggtcca
     8521 cgcccaccca gatcgcgtcg gtcttgcgga agccgtcctc gagctccttg gggagcttgt
     8581 cgggcggctg ctggagcacg tactggaact cgacctgacc ctccacctcg ccgtcgatca
     8641 ggaacgagac gacgtgcacc ccgagctcgg gcaggaccgc gtcgtccacc acgtcgacga
     8701 tctcggtgag gtccatctgg ccgggcatgc ggcgggtcct ggtggtgctg cggtaggcgg
     8761 tggtcccgtt cgggctgatg atctcgaagc cctccaccac gacgccctgc ggcgccttcc
     8821 agtggcggat cacggcgaac ggcagggcgc gcccgggggc gctgaagccg tacacgaacg
     8881 agggcccctc gaccccatcc gcctcgtgga cccgtccgtc caggacgttc ccgaatacga
     8941 gctccgcctt gggcacggcg cgaggatagc agagggggtg gagcggctcg tgatcaggcc
     9001 atgcgggaca gccagagggt gggctcctcc agctcgggca tcgaggggag gctctcggcg
     9061 ctctcccaca tcgaggccag cgcccgctcg tcggtggccc gggccacccg ctccacgaac
     9121 gcgcggccca ggcggaggtg ctcgtcggtg agctcgaacc cgaggaggcg ctcggccatc
     9181 cgctcgtgct cgctccggtc ctcgcgtcgg cggagcatgg cttcctcgat ccgtgcggcc
     9241 tgcgtcagca tccgctcccc gaggacctcc aggacgtgat cggcgtgggc ctcggccgcg
     9301 gccatcagcg cctggacccg ccgcatcagc aggcgctgct cgtcggtgag cttctgcccc
     9361 aggaggtcgg cggggtctcc gatcgcctcc tggagacgct cggggtcgga gaggtccacc
     9421 ccggccagcc gctcctcgag cccggagagg tcgaactcca tcccggccgc gagctcgcgg
     9481 atctgctcca ggaagtgctc ccggacccac cctcggccga gctggaaggc gtgcgtcact
     9541 tcgtggacgg cgacccaggc ccggacgtcg tcgcgaggga ggtcccactc gcgctccagc
     9601 tcctccagcg ccgggaccac gaagaggagg gcgtcctgtc cctcgcgggg cacggccagg
     9661 tcgtactgcc caagggcgcg gcgcccgagc atccccagga ccatcccgac ctgggtgccc
     9721 atgaggagcg gccccatccg gcccatcagc gcgcccaggc cggccaggcc ctccaggccc
     9781 ggggcgccct cgccgccgag ctcgtggccg ggcgcctcca gcatcatctt ccccagcttc
     9841 tcggcgcccg gctcgaagag cccgcggagg ccggtcacgc tggcctcgac ccacttcgcg
     9901 cgccggaccg gtcggatcct ggagacctcg ggcggggcct cgagcccggt gaggtcggcc
     9961 accgcgagct cggcggcgcg gacgaccggc tcgagcatcg ctcgatcctc gtcggtgggc
    10021 tcggggtcgg ggcggtcgcc gaccgcggtg gcgatcccca cctgacgggc cagctcccag
    10081 ttcacggggc cggagccgga gaagagcacc cgctggagct cccggaagag ggggacgtcc
    10141 ttgaagacat cgtcggagaa cggctcgtcg gccatcggct ccattgtagg ctcgccctcg
    10201 ccatgcggat ccgcccggcc gaggagtcgg acctccgcgc cctggccgag atccaggtgg
    10261 ccgggcgcga gtggctggcg cgccgctacg agcccgagac cctgcatcag ttccccgtgg
    10321 acccggacgg ggcggcgttc acgatccggc acgtgcacgg gaccggggcg gtgttcgtgg
    10381 ccgaggatcc cgagcccgtg gggttctcct cggccgtgat ccgcgacggt gtgtggttcc
    10441 tctcccagct gtgggtggcg ccggatcgtc acggggctgg catcgggtcg gccctgctgg
    10501 atgcggcgct ggcctggggc cggggatcct cgaccttcag cgtggtggcg agcccgttcc
    10561 cgggcgccca gaccgtctac atgcggcgct cgatgttccc cgtctggcac tcgatggagt
    10621 tcgagggcga ggcacccggg gtcgagatgc ccgagggagc ggacgagctg acgacggggg
    10681 accagccgtg ggtggacgag ctcgaccgcg aggtgcgcgg catcgccagg cccgaggacc
    10741 acgagctgtt ccgggagcac gccaggggga tcgccctgag gagaggcgac gagccgctcg
    10801 gatatctgta cgcgtggccg gaagggaagc tggggccggc cgcgatccgc gacggccggg
    10861 acacgccgga cctgctccgc gcggggatcg cggccggccc gagccgtctc tcgctcatcg
    10921 tccccacgac gaactgggcc gcgctccagg aggcgatccg actgcgcctc cggctcgtct
    10981 cgaggacggt gttcatggcc agccgcgcca tgcccgacgg gtcccgctac ctctccagcg
    11041 gcggcgccct ggcctgagaa ggagccttcg gtgggtcggg ggctagaatg gccggatgcg
    11101 agccggggat ctcgccatcg atctggggac cgccaacacg ctcgtcttct cccaggagcg
    11161 aggcatcatc ctcaaccagc cgaccgtgct ggccgtgaac gggcgggacg ggtcggtgct
    11221 ggccgtcggc gaggaggcct gggagatgat cggccgcact cccgccaaca tcgtcgccgt
    11281 ccgccccatg cgcagcgggg cgatcaccga ctacgacctc accgcccaga tgatgcggct
    11341 cgtcctgcgc cgcgtcgggg ccacgcggtt ccacaagccc cgggtcctgc tgtgcgtgcc
    11401 gtcggcgatc accgaggtgg agcggcgcgc ggtgagcgag gcctcgatct cggccggcgc
    11461 ccggtcggtc cagctgatcg aggagccgat ggccggggcg atcggggcgg gcctccccgt
    11521 ccatgagccg ctggggaacc tcctcgtgga cgtggggggc ggcacgaccg aggtggcgat
    11581 gatctcgatg ggaggcatcg tcgaggctcg ctccacgagg gtgggagggt tcgacctcga
    11641 cgacgcggtc cagcactacc tccggcagcg gtacgggctg gcggtcgggg agcgtgccgc
    11701 cgagaccgtg aagatcgcga tcgggacggc ctaccctgcc ggcgtccccc cgtactcgat
    11761 ccggggccga gagatcacgt cggggatgcc caaggaggtc cagctcactc aggaggagat
    11821 ccgcgaggcc atctcggacc tcgtcgcgca catcgtcgac gccgcgcggg atacgctcgc
    11881 ctcgagcccg cccgagctgg gccacgacgt tctggagcgc gggatgttcc tcacgggagg
    11941 cggggggctg ctgcgggggc tggacatgcg gatcgcccag gagtgcgagg tgccggtgca
    12001 cctcaccgac tcgccgctgg agacggtcgt gctgggggcg gggaagtgcc tcgacctgcc
    12061 ggccgacgtc ccggggctgt tcgcacagtg acgttctcga tcgtggctgc ggacctggaa
    12121 gcgggggact ggggcgtggc cgtcgcctcg aagttcccgg cggtgggggc cgtggtgccg
    12181 tgggcgcggg cgggcgtcgg cgccgtggct acgcaggcct gggccaacac ctcgttcggg
    12241 cctcgcggcc tcgacctgat ggccggcggg gccgaggcga acgaggcgct caggcagatg
    12301 ctggacgagg acgaggggcg cgtgcagcgg caggtcggga tggtcgattc gcacggcatc
    12361 gtggccacgt tcacgggaga gggttgcatg gagtgggccg gcgggcacac cggggagggc
    12421 ttcgcctgcc aggggaacat cctcgtgggc ccggaggtgg tcgaggcgat ggccgaggcc
    12481 tacgaggcga ccgaagggga tctgctcgac aggctgctgg cggcggtcgc cgccggcgat
    12541 gccgccggcg gcgaccgccg cggacggcag tcggccgcga tcctcgtcgt ccgggaggga
    12601 ggcgggtacg gcggctacac cgaccgatac gtggaccttc ggatcgacga tcaccccgac
    12661 gcggtgaccg agctcgcaag gctcgtcgac gtctacgacc gtgagatcct ggtgcgggac
    12721 gacccgctgc tggaggccac ccccgagctc gtgtccgacc tccagcggcg cctgggccgg
    12781 ctcggacgct accggggcga ggcgcacggc gcgtacgacg aggacacgcg ggcggcgatc
    12841 gaggggttcg ccggcgagta caacctggag ggaaggctcc gagacgacga gatgatctcg
    12901 ggctccctcg tggacgagat ccgggacgtg acgccctaaa gcccgagcat ctggccaagc
    12961 cgctcgatcg cgaagcggcc ggcgaagtac acgcccgcgg cgaccccgag ggccacgatc
    13021 aggatcgaaa ggacgaggag cgtcgcggcg acgaccggat ggatccgctc atcgggggtg
    13081 cttcgcagcg accggagacc ggcgtctgga gcggggggct cgatcacccc ggtccagcgg
    13141 tggtcgtgcg acccctcggt cagctgccag agcagccatc ccacgacggc ggccgtcatc
    13201 accaggagga agagctccat gaccccgatg ttcccatcag aagggattcc atgctccccg
    13261 gcgtacgatt gatccgcatg cgacgactcc tgctctccgc gctgccgctg gtctccctcg
    13321 ccacggccct ctctctcgtg gatatcggca tgttcaccat cgggcctgga tcggccgagg
    13381 aggtcttccc ccggatccag ctcgacggcg tgggcgtcac cgagcccagc ggccggctcc
    13441 tgctgacgac cgtcagcatc cctcgggcca cggtgttcca cgccgtgtgg gcgggatggg
    13501 acccggcggt ggagctggtc cccgaggagg cgatcctggg ccagcgcacc gagcgggagc
    13561 acgagcgcct cacccgatcc cagatggacg agagcaagat cgcggccgtc agcatcgcgg
    13621 ccacggacct caccgactac cccgaggagc acgggccggg cgcgctcgtg cagaacgtcc
    13681 tcggggggac cgccgccgcg gggaagctgc tcccgggtga cctgatcgtg tcggtggacg
    13741 acgatcccgt cgacgacgtg gacgacgtgt cgcgggcgat cgtcgccacc gaagcccggc
    13801 gggccctggc cctcaccgtc gaggccgggg gcgaggagcg cgccgtccgc atccgcccgc
    13861 ggatgtccga ggaggagggc cgtcccatca tcggggtgat cctgatcgag cccttcccct
    13921 tcgaggtcga gatcgagagc gggcggatcg gcgggccctc ggccggcctg atgtggaccc
    13981 tcggcgtcta cgacctcctg tccgaggagg acctggtgga cggacggcgg atcgcgggca
    14041 ccggcgcgat cgacctggcc gggaacgtct acgcggtcgg cggcgccgcg cagaaggtcc
    14101 gcgcggcgag gcaagcgggc gccgaagtct tcctcgttcc gagggagaac ctggaggagg
    14161 cgcggtcggt ggacgccggg atccgggtcg tccccgtccg gaacgtccgg gccgccctgc
    14221 gcttcctccg cggaaccagc tgatgcccgt ctgataggct ggcctggctt tggaccgacc
    14281 tccacctccc cggcgcgcgg cgccccggcg acgctggctg ctgtgggtcg gcggcgccgt
    14341 cctggcgatc gtggtcgtct tcggcgcgct gtccggcttc tacgtggaca tcctctggtt
    14401 ccgcgaggtc gagcagtcga gcgtcttctg gacgatcttc tggtcgaggg tcctcctggc
    14461 gaccgtgttc ggcgctctct tcttcgcgct cctctacgcc aacctgctga tcgcgcgggc
    14521 cgtgcgcccg cgctaccagg tgttcgcgcc gggggaggag gccgtggacc gctaccgcac
    14581 gctcatcgag ccctacgcgc ggtgggcgct cctgggggtc tcggtcctcc tggcgctgtt
    14641 cgccgcggcc ggggtggccg cgcagtggga ggcctaccag cagtggcgcg tggcggggag
    14701 tgtctcgttc ggcgcgaccg atcccgtctt cgagcgcgac gtggccttcc acgtgctctc
    14761 gctcccgttc cagcggttcg tgcagtcgtg gctgttctct tcgttggtgg ccgtcacggt
    14821 ggtgaccgcg gccgcgcact acctgtgggg agggatccga ttccgggccg tggccgagcg
    14881 cgtcaccccg caggtcaagg cgcacctctc ggtgctcctg gggctcatcg tgctggtccg
    14941 ggcctggggc taccgcctgg gtcagttcga cctcctcctc tcgccgcggg gcaccgtcac
    15001 cggcgcctcc tacacggacc tgaacgccca gctgcccgcg ctccagctcc tcgtcgtgat
    15061 ctcgatcatc gtgacggtgc tgttcctggt gaacatccgg ttccgtgggt gggccctgcc
    15121 cgcgatcggg ctggggctgc tggtgctgac ctcggtgctg gccggcgccg tctggcccgc
    15181 cgcgatccag cgcttccgcg tcgaccctca ggaactccag cgcgagcggg agttcatcac
    15241 caggaacatc gacttcacgc ggcaggccta cgggctcgat ggggtcgagg cggagtcgtt
    15301 ccccgcggag ccggagctga cccgtgagga gctcgaggag aacatcggga ccgtggagga
    15361 catccggctg tggaacccga gcactctggc tcgcgcctac ttccagctcc agcggatccg
    15421 cccctactac gagttcgtgg acgtcgacgt ggatcgctac gagatcgacg gcgagaaggt
    15481 gctgacgatg ctcgcgcccc gtgagatcgc gcaggacacg atcccgggcg ggcagacgtg
    15541 gcagaaccgg cacctcgtgt acacgcacgg cttcggtgcg gcggccagca gggcggacca
    15601 gctggacgtg gccggcaccc ctcgcttcct cctgcgggac gttcccccgg cgggcgatct
    15661 ggcggcctcg ctggagcagc cccgcgtgta cttcggggag gagaccgacg tccccttctc
    15721 gctcgtccgc accgagcagc aggagctcga cttcccggtg ggcgagaacg agttcgcgcg
    15781 caccacctac gacggtgagg ggggcatcga ggtgggcggg ttcttccgtc gcctcgcctt
    15841 cgcgtggcgg taccgcgacg tgaacctgct gatctccggg ctcgtgcggg acgacacgaa
    15901 gatcctgatc aacaccgatc tc
//
LOCUS       DASUTW010000308         3050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1659401, whole genome shotgun sequence.
ACCESSION   DASUTW010000308 DASUTW010000000
VERSION     DASUTW010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659401"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VF058_10665"
     CDS             <1..430
                     /locus_tag="VF058_10665"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX6208802.1"
                     /translation="VMLSDRLDDAEVQQRWMAEAVATLGSPDAGVLSLEGAVDGQIQL
                     LWAPDAERMYLVASGMDSVEEGVYHVWLANEDEWWSAGTFVPDEGVAMVPMDDDPGSF
                     DEVAITHEETEEAPRPSATPLAHAEVAEPTAAPSPTPAEG"
     gene            complement(454..1149)
                     /locus_tag="VF058_10670"
     CDS             complement(454..1149)
                     /locus_tag="VF058_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208803.1"
                     /translation="MIAFPLLATLVALVFGAHLALRYARRRAPHEGLWSLAMLMYAGA
                     SAVLALGVQDGWSAGEFRIYWLLGAVLTVPYFAAGELYLLARRRRWIAHLGVAALALA
                     TVWGVIEVWGAPVDRAVLGETFPLGREVFGDGTLPHRLAQYFSFPAYFFLLGGATYSA
                     GRMRGKEELKDRFRGTALIAIGATIVAIGSGVGAGLGNFALFSVSLAAGAAVTYWGFL
                     VATRRRTAAPAAR"
     gene            complement(1146..2651)
                     /locus_tag="VF058_10675"
     CDS             complement(1146..2651)
                     /locus_tag="VF058_10675"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX6208804.1"
                     /translation="MEALPEIVRRAAEADPDHPALIFHDRVTTYADLDRLVDRTAAAL
                     HDLGIVKGDRVAIMLGNIPEFVHAFHGTLRLGAVATPINAMLTPEELGYVLADAEARA
                     VIVAIDLVPAVLAVRDRLGSLDFILVVGPPPSPPGTRSFDDLLDRAKSPPPVDVRRDD
                     LAVLAYTAGTTDQPKGAMLSHGNLLANLEQISEVPILQGAESDVALIALPLFHIYALN
                     AALNLILMEGATAVIVDRFDAAATLRLVPLHGVTILFGVPQMFEAWLAAARRAEPDLS
                     TVRLAVSGAAPLPGAVLEAFEERFDCTIWEGYGLTETAPVVTTNALGERAKPDSIGLP
                     LPGVEVRLLDEDGTDAVEGDPGEILVRGPNVFKGYWRRPEETEEAFTEGWLRTGDIAY
                     RDEDGYLFLVDRSKDLIIVSGFNVYPREVEEALAAHPAVAEAAVIGVPDERTGEAVRA
                     FVVARGEVSNEELLEYCRGRLARFKLPRDIELVDSLPKHATGKVVRRSFRE"
     gene            2747..>3050
                     /locus_tag="VF058_10680"
     CDS             2747..>3050
                     /locus_tag="VF058_10680"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX6208805.1"
                     /translation="MALSATSFASATVMVKLAFREGSGLGVLVLSRSALGAALLWGLA
                     LARGTELRVPAGRWPAVWFMGALSAGVALLLFGATERIPASTTTLLLYVYPAMVAAI"
BASE COUNT          442 a         1085 c         1127 g          396 t
ORIGIN      
        1 cgtgatgctg tcggaccgtc tcgacgacgc cgaggtccag cagcgctgga tggccgaggc
       61 ggtggccacg ctggggagcc cggacgccgg cgtcctctcg ctcgaggggg cggtggacgg
      121 gcagatccag ctgctgtggg cgccggacgc cgagcggatg tacctcgttg cctcggggat
      181 ggattcggtg gaggagggcg tctaccacgt ctggctggcg aacgaggacg agtggtggag
      241 cgcaggaacg ttcgtccccg acgagggcgt ggcgatggtc ccgatggacg acgatccggg
      301 ctccttcgac gaggtggcga tcacccacga ggagacggag gaggcgccca ggccgtcggc
      361 caccccgctg gcccacgcgg aggtggccga gcccaccgcc gccccctccc ccaccccggc
      421 cgagggctga cgcggaccgc aagccccccc gggtcagcga gcggccggcg cggcggtccg
      481 ccggcgggtg gccacgagga atccccagta ggtgacagcc gcgccggccg cgagcgacac
      541 cgagaacagc gcgaagttgc ccagccccgc gcccacgccc gagccgatcg ccacgatcgt
      601 ggccccgatc gcgatcagcg ccgtcccccg gaaccggtcc ttgagctcct ccttgccccg
      661 catgcggccc gcggagtagg tggcgccgcc caggaggaag aagtacgcgg ggaacgagaa
      721 gtactgggcg agccggtgcg ggagcgtgcc gtcgccgaag acctcgcggc ccagcgggaa
      781 ggtctccccg agcaccgcgc gatcgaccgg ggcgccccag acctcgatca ccccccagac
      841 ggtggccagg gccagcgccg cgacccccag gtgcgcgatc caccgtctcc gccgcgccag
      901 caggtacagc tctcccgccg cgaagtacgg caccgtcagc accgctccca gcagccagta
      961 gatccggaac tcccccgccg accacccgtc ctgcacgccc agcgccagca ccgccgaggc
     1021 ccccgcgtac atcagcatcg ccagcgacca cagcccctcg tgaggcgccc gccgccgcgc
     1081 gtaccgcagg gccaggtggg cgccgaagac cagcgccacc agcgtggcca gcagcgggaa
     1141 ggcgatcact cgcggaagct ccggcgcacg accttcccgg tggcgtgctt ggggagcgag
     1201 tccacgagct cgatgtcgcg aggaagcttg aagcgggcca gccgcccgcg gcagtactcc
     1261 agcagctcct cgttcgagac ctccccccgg gccacgacga aggcccggac cgcctccccc
     1321 gtccgctcgt cgggaacgcc gatcaccgcg gcctcggcca cggcggggtg cgcggccagc
     1381 gcctcctcga cctccctcgg gtacacgttg aagcccgaga cgatgatgag gtccttcgag
     1441 cggtcaacga ggaacaggta gccgtcctcg tcccggtagg cgatgtcgcc ggtgcggagc
     1501 cagccctcgg tgaaggcctc ctcggtctcc tcagggcgcc gccagtagcc cttgaagacg
     1561 ttggggccgc ggacgaggat ctcgccgggg tcgccctcca ccgcatcggt cccgtcctcg
     1621 tcgaggagac gaacctcgac gccgggcagc ggcagcccga tcgagtccgg cttggcccgc
     1681 tcgccgagcg cgttcgtggt gacgacgggg gcggtctcgg tgaggccgta cccctcccag
     1741 atcgtgcagt cgaaccgctc ctcgaacgcc tccaggacgg cgccagggag gggggccgcg
     1801 cccgagacgg ccagcctcac ggtcgagaga tcgggctcgg ctctgcgggc ggcggccagc
     1861 caggcctcga acatctgggg caccccgaag aggatcgtga cgccgtggag gggcacgagt
     1921 cggagcgtgg ccgcggcgtc gaagcgatcc acgatgaccg ccgtggcccc ctccatcagg
     1981 atgaggttga gcgcggcgtt gagcgcgtag atgtggaaga gcggcagggc gatcagcgcc
     2041 acgtccgact cggctccctg gaggatcggg acctcggaga tctgctccag gttcgccagg
     2101 aggttgccgt ggctcagcat cgcacccttg ggctggtcgg tggtgcctgc cgtgtaggcc
     2161 agcacggcga ggtcgtcgcg acggacgtcc accggtggcg ggctcttggc ccgatcgagg
     2221 aggtcgtcga acgagcgcgt ccccggcggg gacgggggcg ggcccaccac caggatgaag
     2281 tcgagggagc cgagccggtc ccgcacggcc aggaccgccg gcacaagatc gatggccacg
     2341 atgaccgccc gggcctcggc gtcggccagg acgtacccga gctcctcggg cgtgagcatc
     2401 gcgttgatcg gggtggccac ggccccgagg cgcagggtcc cgtggaaggc gtggacgaac
     2461 tcggggatgt tcccgagcat gatggccacg cggtcgccct tcacgatccc caggtcgtgg
     2521 agggcggccg ccgtgcggtc caccaggcgg tcgagatcgg cgtaggtggt cacccgatcg
     2581 tggaagatca gggccgggtg atccggatcc gcctccgccg ctcgccggac gatctcgggc
     2641 agtgcctcca tcggaggtga gtctaggcta gggtcggtcg gtccggcatg agccagggag
     2701 gaggagcgtc tcgcgcacga ccacccgcct gatcggcatc gtcctcgtgg ccctctcggc
     2761 cacgagcttc gcgtcggcga ccgtcatggt gaagctggcc ttccgcgagg gctcgggtct
     2821 gggggtcctg gtgctgtcgc ggtcggccct cggggcggcc ctcctctggg ggctggccct
     2881 ggctcggggg acggagctcc gggtccccgc ggggcggtgg cccgccgtgt ggttcatggg
     2941 cgcgctgtcg gcgggggtgg cgctgctcct gtttggcgcc accgagcgga tcccggcctc
     3001 cacgacgacg ctgctgctgt acgtctaccc ggcgatggtg gcggcgatct
//
LOCUS       DASUTW010000309         5307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1398197, whole genome shotgun sequence.
ACCESSION   DASUTW010000309 DASUTW010000000
VERSION     DASUTW010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398197"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..509)
                     /locus_tag="VF058_10685"
     CDS             complement(165..509)
                     /locus_tag="VF058_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX6208806.1"
                     /translation="MAYDEDLAARVRDVLAEVDGSTDEREMFGGIAFMVGGHMCCGVT
                     AEDLMLRLGPDRAEEALDEPHVRPMDFTGRPMKGYVYVSAKGLETEEDLARWIGLARA
                     FVAELPPKGKGR"
     gene            complement(574..1659)
                     /locus_tag="VF058_10690"
     CDS             complement(574..1659)
                     /locus_tag="VF058_10690"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX6208807.1"
                     /translation="MDAYIGVGHGRTPGGTLDPGAVFGGLVEHDLAVSVVDAYADAIR
                     RAGLRVTDESNSGPGHDPNFVGSAREANRLRVKYADEVHFNASATHAGTGTEVWVHGG
                     TSEANKRAGRAIAAAISRVLRIPNRGLKLGTFSFLTSTNMPSAIIEVAFLDNTADRRA
                     IRRKDALEEVGEAIAGARCRFLGVTPAQPEPGRRGVSIAGRRPATSRRKAHAWARSKG
                     AAPVFHEIIDAGWRQARKIGIDAAVFVAQAAKETGFGRFGGVIDASFHNSCGLKTAAG
                     GDNDDPGAHQRFRDWEAGTRAHCAHLALYAGVLRPAEARRLGDPRAFTSIHGVAPTVQ
                     ELGGKWAPARDYGRSIVRDLYRPLRRF"
     gene            complement(1760..3427)
                     /locus_tag="VF058_10695"
     CDS             complement(1760..3427)
                     /locus_tag="VF058_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015624663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX6208808.1"
                     /translation="MSRAYDVLVIGSGFGGSVAALRLAEKGYRVGVLEAGRRFGPRDF
                     AARNWNLRRYLWLPRLGLRGIQRLTLLRDALVLSGAGVGGGSLVYAGVLYDPPEPFWD
                     DPGWPGVVDWRRELAPHYDQAKRMLGAAVNPFETPADHVLHQVAARLDASDTHRPTTV
                     GVHFGEPGRRVPDPYFGGAGPTRAGCIGCGGCMVGCRFDAKNSLDRNYLWLAESLGAV
                     IHPETEAVDVIPLPGGGYQVETRRPGSWSGDRRSFRAREVVFAAGVLGTLKLLLKLRG
                     AGRLRGLSARLGHLVRTNSEVIVGAVARRPDVDYSTGVAITSSIQPEPDTHVQVVRYP
                     RGSNFMGLIGTILVDRDERRSRQLSFLAQALRRPVRFARSLSAYRWSERSVILLVMQS
                     RDNSLRLVLRGRRLTSVAGEGTPSPRYIPVANRAARLAAGIIGGEPAGALNEALLDVP
                     VTAHMLGGARIGTSSEDGVIDPYHRVFGHAGLHVMDGSTIAANLGVNPALTITALAER
                     ACSFWPNAGEADPRPELGRYEHVASVPSRRTAVPEGAPASLFYEDGR"
     gene            complement(3514..3909)
                     /locus_tag="VF058_10700"
     CDS             complement(3514..3909)
                     /locus_tag="VF058_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208809.1"
                     /translation="MDVDLAAGTVTLSGSNMSLTSIEQAAGTICCNDTLDDAGPNELF
                     GFAGNDILTGAEGDDLLNGEGGTDSGDGEDGEADRCISIENPSNCELFEPAAAAQTTR
                     ALNSPEPTVRRPLAHAMDGEVVRMPLVGL"
     gene            complement(4087..4728)
                     /locus_tag="VF058_10705"
     CDS             complement(4087..4728)
                     /locus_tag="VF058_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020462932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HEX6208810.1"
                     /translation="MAFRGWPVEALEFYEGLEADNTKGYWQEHRHVYDGLVRAPMEAL
                     LEELSPEWGDWKIFRPYRDIRFSPDKSPYKTHIGATIGSGYVQLSANGLAAGCGMYQM
                     ARDQLERYRAAVGAERTGRELVELVDAVRADGIEITAHDVLKTAPRGHPKDHPRIELL
                     RLKGLITWREWPAAAWMGTPKAKDRIVEFLRRSKPIADWLESHVGPSTLPNER"
BASE COUNT          779 a         1936 c         1787 g          805 t
ORIGIN      
        1 gaagctcgcg atgttcccct cgcatacgaa cgtcggcgtg ctccacttga tcgtctcggt
       61 gacgcggtcg tccgcgccga ggatgatctc gcgagcccgc cgcatcgcgg cgtcgagcgg
      121 atgatcccgc tccacgagcc agcggtcgac ctccgggttc gtgttcatcg tccctttccc
      181 ttgggaggga gctccgccac gaacgcccga gcgagcccga tccaccgtgc cagatcctct
      241 tcggtctcca ggcccttcgc cgagacgtac acgtagccct tcatcgggcg gccggtgaag
      301 tccatgggcc ggacgtgcgg ctcgtccagc gcttcctccg cccgatcggg gcccagcctc
      361 agcatcagat cctcggcggt caccccgcag cacatgtgac cccccaccat gaacgcgatc
      421 ccgccgaaca tctcccgctc gtccgtgctc ccgtcaacct ccgcgaggac gtccctcacc
      481 ctcgccgcga ggtcctcgtc ataggccatc gtgcctcctc gggtcccgca ccatcatgcc
      541 cctccacacc gacgaagggc tccccggcga gtcctagaac cggcggagag gccggtagag
      601 gtcgcggacg atgcttcggc cgtagtcgcg agccggggcc cacttccccc cgagctcctg
      661 aaccgtcggc gcgaccccgt ggatcgaggt gaaggcccgc gggtctccca gcctcctggc
      721 ctcggccggc ctcagcacgc ccgcgtagag cgcgaggtgc gcgcagtgcg ccctggtccc
      781 cgcctcccag tctcggaacc gctggtgcgc accgggatcg tcgttgtccc ctcccgcggc
      841 ggtcttcagg ccgcacgagt tgtggaagct cgcgtcgatc acgcccccga accttccgaa
      901 gccggtctcc ttggcggcct gggccacgaa gacggcggcg tcgatgccga tcttccgagc
      961 ctggcgccat cccgcgtcga tgatctcgtg gaagacgggg gcggccccct tcgatctggc
     1021 ccaggcgtgg gccttccgcc tggacgtcgc ggggcggcgc ccagcgatcg agacccctcg
     1081 cctgccgggc tcgggctggg ccggcgtgac gccgaggaac cgacagcgcg ccccggcgat
     1141 cgcctcgccc acctcctcca gcgcgtcctt ccgccggatg gccctccggt cggcggtgtt
     1201 gtccaggaag gccacttcga tgatggccga gggcatgttc gtcgaggtga ggaagctgaa
     1261 cgtcccgagc ttcagcccac ggttggggat gcggaggacc cgggagatcg cggcggcgat
     1321 ggcgcggccc gcccgcttgt tcgcctccga cgtgcccccg tgcacccaga cctcggtgcc
     1381 ggtgccggcg tgcgtggcgg aggcgttgaa gtggacctcg tccgcgtact tcacgcggag
     1441 ccgattggcc tccctggccg agcccacgaa gttggggtcg tgccccggcc cggagttgct
     1501 ctcgtcggtg acacggaggc cggcgcgacg aatcgcgtcc gcgtaggcgt ccaccacgga
     1561 gacggcgagg tcgtgctcga cgaggccgcc gaagaccgcc cccggatcca atgtgccgcc
     1621 cggcgttctc ccgtgcccca ccccgatgta ggcgtccatg gcgcacctcc tcgatacggg
     1681 ccggcacccg tcccacgacg agtggagcaa ggctaggagc gtgcgagcgg ccggtcaatc
     1741 gggcggatgg gggagaggct cagcggccgt cctcgtagaa gaggctcgcc ggggcccctt
     1801 cggggaccgc cgttcgcctc gaaggaacgg aggcgacgtg ctcgtagcgc ccgagctccg
     1861 gccgggggtc cgcctccccc gcgttgggcc agaacgaaca ggcccgctcc gccagggccg
     1921 tgatcgtgag cgccgggttc actccgaggt tcgcggcgat cgtggagccg tccatcacgt
     1981 gcaggccggc gtggccgaac acccggtggt agggatcgat cacgccgtcc tccgaggagg
     2041 tgccgatgcg ggcgccgccg agcatgtgag ccgtcaccgg gacgtcgagc agggcctcgt
     2101 tgagtgcgcc ggcgggctcg cccccgatga tcccggccgc caatctggcg gcgcggttgg
     2161 cgacggggat gtaccgaggg ctgggcgtgc cctccccggc gaccgaggtg agccgtcgtc
     2221 ccctcaggac gagccgcagg gagttgtccc gactctgcat cacgagcagg atcaccgacc
     2281 gctccgacca ccggtaggcg gagaggctgc gtgcgaaccg cacggggcgc cgcagggcct
     2341 gggccaggaa cgaaagctgg cgcgacctgc gctcgtcccg gtccacgagg atcgtgccga
     2401 tcaggcccat gaagttgctg cctctcgggt agcggactac ctgcacgtgg gtgtcgggct
     2461 ccggctggat ggacgaggtg atcgcgaccc ccgtcgagta gtcgacgtcc ggcctccgcg
     2521 cgacggcccc cacgatcacc tcggagttcg tccggaccag gtggcccagg cgcgcggata
     2581 gcccccgcag ccgccccgcc cccctgagct tcaggagcag cttcaaggtt ccgagcacgc
     2641 cggccgcgaa cacaacctcg cgggctcgga aggaacggcg gtctccggac cagctccccg
     2701 gccgtctcgt ctcgacctgg tacccgccgc cggggagcgg gatcacgtcg acggcctccg
     2761 tctcgggatg gatcacggct cccaagctct cggccagcca gaggtagttc cggtcgaggg
     2821 agttcttggc gtcgaagcgg caccccacca tgcaccctcc gcaaccgatg cagccggccc
     2881 gagtcggacc cgcgccgccg aagtacgggt ccggcacccg cctgcccggc tcgccgaagt
     2941 gcacccccac ggtcgtcgga cgatgcgtgt cgctcgcgtc cagccgagcg gcgacctgat
     3001 ggagcacgtg gtcggccggc gtctcgaacg ggttcacggc ggccccgagc atccgcttgg
     3061 cctggtcgta gtggggggcg agctcgcgcc tccagtcgac gactcccggc cagcccggat
     3121 cgtcccagaa gggttccggc gggtcgtaca ggacccccgc gtagacgagg gaacctccac
     3181 ccacgcccgc ccccgagagc acgagcgcgt cgcggaggag cgtgagccgc tggatgcccc
     3241 gcagcccgag cctggggagc caaaggtacc gccggaggtt ccagttgcgc gcggcgaagt
     3301 ctcgcggtcc gaagcgccgc cccgcctcga gcacgcccac ccggtagccc ttctcggcga
     3361 gcctgagcgc ggcgacgctg cccccgaacc cgctgccgat gacgaggacg tcgtacgccc
     3421 ggctcaagag ggccctgtcc tcctagacgc ggaacagagg gatcgtcatg gggtcggact
     3481 ctagcagcgg agcggtgagc gggatgtcgt ggctcagagt ccgacgagag gcatgcggac
     3541 cacctcccca tccatcgcat gtgctagtgg gcgccgcacg gtcggctccg gtgagttcaa
     3601 cgcccgcgtc gtctgcgcgg ccgcggcggg ctcgaagagc tcgcagttgc tcgggttctc
     3661 gatgctgatg cagcgatccg cctcgccatc ctctccgtcg ccggagtcgg tgcccccctc
     3721 gccgttgagg agatcgtcac cttcggcacc agtcaggatg tcgttccctg cgaacccgaa
     3781 cagctcgttc gggcctgcgt cgtcgagggt gtcgttgcag cagatcgtcc cggccgcctg
     3841 ctcgatcgag gtcagggaca tgttcgaccc gctcagcgtg acggtccccg cggcgaggtc
     3901 cacgtccacg cccgtgccga cgagcagcag gtacgccgcg gtccatcttg tgaccgctca
     3961 ggcgtgctga gagccgcctc acccgaatgc cgaagaacgc catgacccgc cctccctctc
     4021 gaaacgcccg aatggtcgtc gattgttcta cagaaaggca gtgctggccg cgtcggtgag
     4081 agcgcctcat cgctcgttcg ggagcgttga ggggccgacg tgcgactcga gccagtcggc
     4141 gatcggcttc gaccggcgaa ggaactccac gatccggtcc ttggccttcg gcgtgcccat
     4201 ccacgccgcg gccggccact cgcgccacgt gatcagaccc ttcagacgca gcagctcgat
     4261 gcgaggatga tccttcgggt ggccgcgcgg ggcggtcttc agcacgtcgt gggcggtgat
     4321 ctcgatcccg tccgctctca ccgcgtccac cagctcgacc agctcgcgac ccgtccgctc
     4381 cgcgcccacc gccgcccgat agcgctcaag ctggtcgcgc gccatctggt acatgccgca
     4441 ccccgcggcg aggccgttgg cgctgagctg cacgtagccg gagccgatcg tggcgccgat
     4501 gtgggtcttg tacggcgact tatcggggct gaagcggatg tcgcggtagg ggcggaagat
     4561 cttccagtcg ccccactcgg gggagagctc ctcgagcagc gcttccatcg gtgcgcggac
     4621 cagcccgtcg tagacgtgcc ggtgctcctg ccagtacccc ttcgtgttgt cggcctccag
     4681 gccctcgtag aactcgagcg cctccaccgg ccaaccgcgg aacgccatcg tcatctctcc
     4741 ccgaggcggc gagtctacct cgccgcggtc agtcgaaggc caggccctcc gtggacgaca
     4801 gactccagat cgggcaggcg catcaggaag gagttcgcct ttcaccaggt caggcagttc
     4861 cggaactagc cgcgacaaca gatctcagac ggcgtgccac ccggcgcgtg aacccgatgg
     4921 gtccaccgcg agagcgcacc gcgcacccag ctctctgcac ccacgtagcg ttcagtagcc
     4981 ggcgggtctc atccgggccg ccgcgaggaa cgccacctgg cgctgcggca ggcgaggcga
     5041 tgaccacccc ctccatcatg gattcctcgc ccggccagag ggcgttactc gcggtctgca
     5101 cttcccagcc cgtctccacg aacaggcgct gataggagga tctgaaccag ggactctcct
     5161 tcggacgtgc tggggatcgc cggcttcagg aggacgcgcc gatcgacgcc tcctgctcga
     5221 gacgcgtccg atcgaccttg ccggtggaca ttcgaggaag tcccgagacg atctgcactc
     5281 gggcggggac catgtaacgg gggaggc
//
LOCUS       DASUTW010000310         6403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1530745, whole genome shotgun sequence.
ACCESSION   DASUTW010000310 DASUTW010000000
VERSION     DASUTW010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530745"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1842)
                     /locus_tag="VF058_10710"
     CDS             complement(<1..1842)
                     /locus_tag="VF058_10710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HEX6208811.1"
                     /translation="MTEGAAVPQSNAAQEEIDEVADLPRIGHRPCIQGLAGPFPCHMV
                     DLMTFLPMDDIGGGPGSIYGGPGNDVWGWTDPETHAEWIIAGRSNGTSFIDASDPKNP
                     VFVADLPAHGVTELHSDMKVYRGHAYIVKDGPGNGIQVFDLTRLRDIDYGDAPVTVDA
                     DAVYEEISNAHDIAINEDTGFAYALGATGGAIGQLANVVTIDAPSSAAGTYQAAGANF
                     GPAPDATGVSGDVVLVSDGVGTTTDACQPLVGFPAGSIALVDRGACNFTVKANNAQAA
                     GAAAMIVANNVGTNPFTMGGSDPSITIPSVMISLADGNTIKAGLPASGTVSSNPEANP
                     CDRGGLHMVDIRDPKNPTYAGCFDDDGYTHDGQCVVYRGPDERYQGRELCFAANPSSP
                     GVGDAVTIVDVTDKSNPVLVGKVFEGEPNTYSHQGWLTPDHRYFLHDDESDNGGSSQA
                     IQGRTRTRVFDVTDLENPFLQAVYHHPTRAPAHNLYTRGRYMYNAHYIDGLRIVDITK
                     IDHPGDEFLAQTPSAPDHQGLYEVGCFDTDPFRNDMGVFPNGRTAWGGSWSNYPYFKS
                     GVVVVSGFDGLFLVKPRLGVPAESPPAQGKNPPTGNAGGNSTNVCLPD"
     gene            complement(2089..2685)
                     /locus_tag="VF058_10715"
     CDS             complement(2089..2685)
                     /locus_tag="VF058_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX6208812.1"
                     /translation="MSAVRWIHVVSAIVWPASAALGLWRARSLIRRRTRARVYALMAV
                     AIAVGAENLIQALSPHHPEGLAHLIANIVLAAAGVVALFVLAAYPEEIAHEERVLYAL
                     SRRNLGAGDGDPRPARPLSRRELEVLESLCGGLSTDEIAGHLSISRHTVATHVRNLMG
                     KLGVGSRVDAVSWAIRQGIYDPAEGRITPESQPLPRTR"
     gene            complement(2808..4037)
                     /locus_tag="VF058_10720"
     CDS             complement(2808..4037)
                     /locus_tag="VF058_10720"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase regulatory domain-containing
                     protein"
                     /protein_id="HEX6208813.1"
                     /translation="MRLSFQQLADRAGVDEDFVRRLHAMGALHTSQEPFGERDLHLVA
                     LLHRWEEAGIGAETVLRATEQGDLSLSFLATPGFELTGRLERTYRQYAEEAGVPLDLI
                     LSLHEAIGFARPDPDDRIREDDPILVEMAAAFLESGASETAVRRMFRIYGDNLGRLAA
                     AIGDVYRAEIEQRFREGGATDTDVMERGARLGRRVGALMHQALVAIFERHHQHVWTGY
                     SIGWAEASLERVGLRQRVERPPAICFVDLTGYTRFTEEQGDEAAARLAASLAALVEEI
                     SLRRGGRAVRWLGDGGMFLFDDPKAAVLAGLDMAMRAPAEGLPPTHVGIQAGPVIFQD
                     GDVYGRTVNIASRIAARAGPGEVLVGAEVAELAHGPEVRFEPIGPAELKGLAEPVTLY
                     RARRAAEHGGAPTGRGA"
     gene            complement(4062..4262)
                     /locus_tag="VF058_10725"
     CDS             complement(4062..4262)
                     /locus_tag="VF058_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208814.1"
                     /translation="MTFLDGILATATLTGLALNAVAGWWWADPAAAILVAVAALNEAR
                     ENVAEAREIVGAGGQSLRAPKR"
     gene            complement(4446..5147)
                     /locus_tag="VF058_10730"
     CDS             complement(4446..5147)
                     /locus_tag="VF058_10730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6208815.1"
                     /translation="MTGAIRAPSGGGSTTEPARTGPIFDRLAERYDAWYEGPAGSVAF
                     PQEVECLAPLLSGAPGPRLEIGVGSGRFAQALQIELGLDPARAPLLLARDRGVRVVQG
                     VGERLPFQDGSLGGIVVVVTLCFADDPGELLAEARRVLRPGGSLVLGMVFADSPWGDW
                     YRERGEAGHTFYSAARFLTRREVRAMLETAGLTVGAARSTLLQPPSDAPRPEPSRDGD
                     DSRAGFVGWKAIPLG"
     gene            complement(5144..5602)
                     /locus_tag="VF058_10735"
     CDS             complement(5144..5602)
                     /locus_tag="VF058_10735"
                     /inference="COORDINATES: protein motif:HMM:NF022219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061136 - regulation of proteasomal protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome activator"
                     /protein_id="HEX6208816.1"
                     /translation="MAGRLIVVGRGAPLPKARAAEAVEHPGKLLRLATMIRSVLEEMR
                     GIPLDEGSRSRLREVFDHTVAQLEGLVSKDLREELEALAPPIEGLPSESEIRVAQAQL
                     MGWLEGLLHGIEAAVWAQFVASQAQLEEVRRRALQAREEQEQEADRGRYL"
     gene            5721..6248
                     /locus_tag="VF058_10740"
     CDS             5721..6248
                     /locus_tag="VF058_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208817.1"
                     /translation="MSTTLSHRPQVTPPPGLGEIRRAMHHHVLAVSSMLPRATGWDEL
                     RDHCRTLLSGDLFTFAASTIREEALLVHGRHAPASRRRLFRDWLVAVSVLPWSAPGHP
                     GRYLALLAGLSEPAYEHPEGRHVAHWMAFDDGGELTVRLTVIPNHRVSAAPLLLPVDL
                     LAPAERKLLRLPAAA"
BASE COUNT          910 a         2356 c         2170 g          967 t
ORIGIN      
        1 gtcgggcagg cagacgttgg tgctgttgcc gccggcgttg ccggtgggtg gattcttgcc
       61 ctgggccggc ggcgactcgg cgggcacacc cagtcgcggc ttgacgagga acagcccgtc
      121 gaagccgctg acgaccacga ccccgctctt gaagtacggg taattgctcc acgagccgcc
      181 ccaggccgtc ctcccgttcg ggaacacgcc catatcgttg cggaaggggt cggtgtcgaa
      241 gcacccgacc tcgtagaggc cctgatggtc gggcgcggac ggcgtctgcg ccaggaactc
      301 atcgcccggg tggtcgatct tcgtgatgtc gacgatccgc agcccgtcga tgtagtgcgc
      361 gttgtacatg tagcggcccc gcgtgtagag gttgtgcgcg ggggcgcgcg tcgggtggtg
      421 gtaaaccgcc tgcaggaacg ggttctccag gtcggtcacg tcgaacaccc tcgtccgtgt
      481 ccggccctgg atcgcctgcg agctgccgcc gttgtcgctc tcgtcgtcgt gcaggaagta
      541 ccggtggtcc ggagtcaacc acccttggtg gctgtacgtg ttcggctcgc cctcgaagac
      601 cttgccaacc agcaccgggt tcgacttgtc ggtgacgtcg acgatcgtca cggcgtcgcc
      661 gacccccggc gagctcgggt tggccgcgaa gcagagctca cgcccctggt agcgctcgtc
      721 ggggccccgg tagacgacgc actgcccgtc gtgcgtgtac ccatcgtcgt cgaagcagcc
      781 ggcgtaggtc gggttcttcg ggtcccggat gtcgaccatg tggagccctc ccctatcgca
      841 ggggttggct tccggattgc tgctgaccgt gccggacgcg ggcagacccg ccttgatcgt
      901 gttgccgtcc gcgagcgaga tcatcaccga cgggatcgtg atcgatggat cgctaccccc
      961 catcgtgaac gggttcgtgc ccacgttgtt ggccacgatc atcgcggcgg ccccggcggc
     1021 ctgggcgttg ttcgccttaa ccgtgaagtt gcacgcgccg cggtccacca gcgcgatcga
     1081 accggcgggg aacccgacga gcggctggca ggcgtccgtg gtcgtcccca cgccgtcgct
     1141 caccagcacc acgtctcccg agacaccggt tgcgtcgggc gccggtccga agttcgcccc
     1201 agcggcctgg tacgtgcccg ccgccgacga tggcgcgtcg atcgtgacga cgttcgccaa
     1261 ctgtccgatc gcgcccccgg tcgcgccgag cgcgtaggcg aagcctgtgt cctcgttgat
     1321 cgcgatatcg tgcgcgttgc tgatctcctc gtagacggcg tccgcgtcga ccgtgaccgg
     1381 agcgtccccg tagtcgatgt cacgcaggcg ggtgaggtcg aagacctgga tcccgttgcc
     1441 agggccgtcc ttcacgatgt acgcgtgccc cctgtagacc ttcatgtcgc tgtggagctc
     1501 ggtcacccca tgggcgggca ggtcggcgac gaacacgggg ttcttcgggt cgctggcgtc
     1561 gatgaacgag gtcccgttcg acctacccgc gatgatccat tcagcgtgag tctccgggtc
     1621 ggtccagccc catacgtcgt tgcctgggcc gccgtagatg ctgccggggc cgccgccgat
     1681 gtcgtccatg ggcaggaagg tcatgagatc gaccatgtga caggggaacg ggccggcgag
     1741 gccctggatg cacggccgat ggcctatgcg cggcaggtcg gcgacctcgt cgatctcctc
     1801 ttgcgccgcg ttgctctggg ggaccgccgc gccttcggtc accgcgcctt cgtctgggtg
     1861 cgccgtcgcg ggcaccccag ccgccaccag gagcccgacg gcgagcagca atccggcggt
     1921 ccgcgcggca ctccgccggc gcggtgccgg ccacttcaac ggaaacgtca tcgcgattcc
     1981 tcctttcgcg tgccggtcgc gtgtcaccga agggcatgaa ccgcgtcccg aacaatcgca
     2041 tgacgcgagt aacttcctac caggttgggg gtagcgtcgc cccgagggct acctagtccg
     2101 tggtaggggc tggctctccg gggtgatccg tccttcagcc gggtcgtaga tcccttgacg
     2161 gatagcccaa ctgaccgcat ccacgcggct ccccaccccc agcttcccca tgaggttgcg
     2221 gacatgggtc gctaccgtat ggcgggagat ggacagatgc ccagcgatct cgtcggtaga
     2281 gagccctcca cacagagact ccaggacctc cagctcccgc cgggagaggg gtcgggctgg
     2341 gcgagggtct ccgtccccgg cgcccaagtt gcggcgggag agcgcataga ggactcgctc
     2401 ctcgtgcgcg atctcctccg ggtaggcggc caggacgaac agggcgacca cgcccgcggc
     2461 cgccagaacg atgttggcga tcagatgggc aagaccctct ggatggtgtg gggataacgc
     2521 ttggatcagg ttctcggcac cgacggcgat ggccaccgcc atgagcgcgt acacccgggc
     2581 ccgggtcctg cgccggatca gggaccgggc acgccagagc ccaagagcgg cgctggccgg
     2641 ccacacgatc gcgctcacca cgtggatcca gcggacggcc gacatcaagc cgagcataag
     2701 acttccagcg gctccgggcg atctcgcgtc gccttgtgag gacagcccgc ggcccgagcc
     2761 gcggtctgca ttcgtgtttg accccatcgg tcggggtttc tgaatcctca cgcaccacgt
     2821 cccgtcgggg cgcccccgtg ctcggcagcc cgccgggctc gatacagggt cacgggctcg
     2881 gcgaggccct tcagctcggc cggaccgatc ggctcgaacc gcacctccgg gccgtgggcg
     2941 agctccgcga cctccgcacc gacgaggacc tcgcccggcc ccgcgcgcgc cgcgatccgg
     3001 gaggcgatgt tgacggtccg gccgtagacg tcaccgtcct ggaagatcac gggccccgcc
     3061 tggatgccca cgtgagtcgg ggggaggccc tcggcggggg ccctcatcgc catgtccagg
     3121 cccgcgagga ccgcagcctt cggatcgtcg aagaggaaca tcccgccatc ccccagccag
     3181 cggaccgccc gacccccgcg acggagggag atctcctcca cgagcgcggc gaggctcgcc
     3241 gccagccggg cagcggcctc gtccccctgc tcctcggtga agcgcgtgta gccggtgagg
     3301 tccacgaagc agatcgccgg cggtcgctcc actcgctgac ggagccccac ccgctccagc
     3361 gaggcctccg cccatccgat cgagtacccg gtccacacgt gctggtgatg gcgctcgaag
     3421 atcgccacca gggcttggtg catcagcgcg ccgacccgcc gcccgagcct ggctccacgc
     3481 tccatgacgt cggtgtcggt cgcgccgccc tcacggaatc gctgctcgat ctcggcccga
     3541 tacacgtcgc cgatcgccgc ggccagccgc ccgaggttgt ctccgtagat ccggaacatc
     3601 cggcggaccg cggtctccga cgccccgctc tcgaggaagg ccgcggccat ctcgaccagg
     3661 atcgggtcgt cctcgcggat ccggtcgtcg ggatcgggcc gggcgaagcc gatcgcctcg
     3721 tgcaggctca ggatcaggtc gagggggacc ccggcctcct cggcgtactg ccgataggtc
     3781 cgctccaggc gccccgtgag ctcgaacccg ggggtcgcca ggaacgatag cgagaggtcc
     3841 ccctgctccg tcgccctgag gacggtctcc gcaccgatcc ccgcctcttc ccatcggtgc
     3901 aggagggcca cgaggtgcag atcgcgctcg ccgaagggct cctgcgatgt gtgaagcgcc
     3961 cccatcgcgt ggagccgccg gacgaaatcc tcgtccaccc cggctcgatc ggcgagctgc
     4021 tggaacgaca ggcgcacgct caggttcctc ctccggacac gctaccgctt cggcgcccgg
     4081 aggctctgcc ccccggcacc cacgatctcc cgggcctcgg ccacgttctc tcgcgcctcg
     4141 ttcagcgccg ccaccgcgac caggatcgcc gccgccgggt ccgcccacca ccaccccgcg
     4201 accgcgttga gggcgagtcc ggtcagggtg gccgtggcca ggatcccgtc caggaaggtc
     4261 atcgcggcct cggatcgaag aggtgccgaa tccagctcgt cggccgttcg acgcttcgcg
     4321 acggccaagc gtgtcccacg ctcacgtcgg gggccctcac cggagacgag aggcgaggaa
     4381 cccgctcccg ttcccgtgtc gacgcccctg gccttccacc ggaccgtgct ccgggggagc
     4441 gggcgtcagc cgagcgggat cgccttccag cccacgaacc cggccctgga gtcgtcgccg
     4501 tccctcgacg gctcgggccg aggggcgtcc gacggcggct gcaggagggt cgagcgggcc
     4561 gccccgacgg tgagccccgc cgtctcgagc atcgctcgca cctccctcct ggtcaggaac
     4621 cgggcggccg agtagaaggt gtggccggcc tcgcccctct cccggtacca gtcaccccac
     4681 ggcgagtcgg cgaagaccat ccccagcacc agcgacccgc ccggtcggag cacccgccgc
     4741 gcttccgcca gcagttcccc cggatcgtcc gcgaagcaga gcgtgaccac gaccacgatc
     4801 cccccgaggc tcccgtcctg gaaggggaga cgctccccaa caccctggac cacgcggacg
     4861 cctcgatctc gggccaggag aaggggtgct cgcgccgggt ccagcccgag ctcgatctgg
     4921 agggcctgag cgaatcggcc gctccccacg ccgatctcga gccgcggccc tggcgcgccc
     4981 gacagcagcg gggcgaggca ctcgacctcc tgtgggaagg ccacgctccc cgcgggcccc
     5041 tcgtaccagg cgtcgtaccg ctcggccagc cgatcgaaga tggggcccgt gcgggcgggt
     5101 tcggtcgtgg acccgccccc ggagggcgct cggatcgctc ccgtcacagg tatcgtcccc
     5161 tgtccgcctc ctgctcctgc tcctcgcggg cctggagggc gcggcggcgc acctcctcga
     5221 gctgagcctg agatgccacg aactgggccc acaccgccgc ctcgatgccg tgcaggaggc
     5281 cttccagcca ccccatgagc tgggcctggg ccacgcggat ctcggactcg ctcgggagcc
     5341 cctcgatcgg cggggccagg gcctcgagct cctcacgcag gtccttcgac acgaggccct
     5401 cgagctgggc caccgtgtgg tcgaagacct cgcggagacg cgacctgctc ccctcgtcca
     5461 gcgggatgcc gcgcatctcc tccagcacgc tgcggatcat ggtcgcgaga cggagcagct
     5521 tccccggatg ctccacggcc tccgcggcgc gcgccttcgg aaggggtgcg ccccgaccga
     5581 cgacgatcag ccttcccgcc attgtccctt cactctagga agggggccct ccgtccactc
     5641 ccttccgacg ctcgtgcggc tagcgtgccg acacgagcga acggggcggg gccggggacc
     5701 tccccgaggg aggaggaccg atgtccacga cgcttagcca ccgtccccag gtcacgccgc
     5761 ccccgggcct cggagagatc cgccgggcca tgcatcacca cgtgctcgcc gtgtcctcga
     5821 tgctgccgcg ggccaccggc tgggacgagc tgcgcgatca ctgtcgaacc ctgctctcgg
     5881 gggatctctt cacgttcgca gcgtcgacga tccgcgagga ggccctgctg gtccacggga
     5941 ggcacgcccc cgcgagccgg cggcggctct tccgggactg gctggtggcg gtcagcgtgc
     6001 tcccctggtc cgcccccggc cacccgggca ggtacctggc gctgctggcc ggcctgagcg
     6061 agcccgccta cgagcacccc gaggggcgcc acgtcgccca ctggatggcg ttcgacgacg
     6121 gcggggagct caccgtgcga ctcacggtga tcccgaacca ccgcgtctcc gcggccccgc
     6181 tcctcctgcc ggtggatctc ctcgcgccgg ccgagcggaa gctcctccgg ctcccggccg
     6241 ctgcctgatc cgggggagaa tgggacgaga cggcccgggc gggccgggac ggaggtgagg
     6301 aggatgtcca gggtcaccga ccacctggag cagcgggggg tcagctacga ggtgatgacc
     6361 cacgagcgca cgtacacctc gatcgaggag gcccgcgcgc tgg
//
LOCUS       DASUTW010000311         3426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1011461, whole genome shotgun sequence.
ACCESSION   DASUTW010000311 DASUTW010000000
VERSION     DASUTW010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011461"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /gene="rplU"
                     /locus_tag="VF058_10745"
     CDS             <1..494
                     /gene="rplU"
                     /locus_tag="VF058_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX6208818.1"
                     /translation="QMYAVIKTGGKQHKVRPGDVIEVEYMDVDPGSAVEFEPILVMDD
                     KNKPHVGKSLGNAKVVATLVGDKKAAKIKVVKYRAKSGYKRNQGHRQRMTVLEIQEVK
                     FGGRAGKKDEEEPKAAAKKAAAKKSTRKKAAAKKSSAKKTSAKKSSKKSASKKSSSRK
                     KAT"
     gene            514..762
                     /gene="rpmA"
                     /locus_tag="VF058_10750"
     CDS             514..762
                     /gene="rpmA"
                     /locus_tag="VF058_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX6208819.1"
                     /translation="MAHKKGAAASRNGRDSTSKRLGVKAFGGETVKAGEIIVRQRGTK
                     VHPGLNVRRGGDDTLFAVAPGSVRFYSSNGRKYVTIEA"
     gene            complement(746..1132)
                     /locus_tag="VF058_10755"
     CDS             complement(746..1132)
                     /locus_tag="VF058_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208820.1"
                     /translation="MPKRTIAWLWRPGESKERRGTLSLEGETPTFRSDDGEEVRFGEV
                     ERARRQRGTPVLEIRYRREGERRMAFLFFARPPERPDPEPVPSLFGIVGIRGMRRVGD
                     MGRIRAANRRLKPLIEEWEEALRPRW"
     gene            1153..2391
                     /gene="obgE"
                     /locus_tag="VF058_10760"
     CDS             1153..2391
                     /gene="obgE"
                     /locus_tag="VF058_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX6208821.1"
                     /translation="MSEFVDEAVVYARGGRGGDGAASFHREKYRPKGGPDGGDGGRGG
                     DVILEASPDVFDLASVARHPHQRAEDGGHGRGDNRQGADGDDLVIPVPDGVFVEEERG
                     PVADLVGPGARVVVARGGRGGRGNASLASGRNRAPQTAERGEPGEERRLRLELRLVAD
                     VGLVGLPNAGKSTLLSALTAARPKVADYPFTTLTPNLGVAEGERRVVLADVPGLIEGA
                     HEGRGLGHRFLRHVSRSRALVFVVDVAGEDPAADLRLVRAEVAAYDPELPERPWLVAA
                     TKADLLEAEEAERRAEALREEGETHLVSGATGEGLDRLAERLGRLADESTVERKPFVV
                     LRPGREPFTVRREGDGFRVEGRIVERWVEEANLDDPAQVVALQDRLRREGVERKLEEL
                     GARRGDEVRIGDQAFEYHPG"
     gene            2401..2829
                     /locus_tag="VF058_10765"
     CDS             2401..2829
                     /locus_tag="VF058_10765"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6208822.1"
                     /translation="MTKDELFRLEQDAWVPLHDLVHGLDPESAERPLTDLWTVRDGLA
                     HIACWQAEAAQMLLQIREGTYEGWDGDEDEVNARFHEATKDLPLDEVMASLHASRSRM
                     LEELDRLPDDLLDDEARSWFVESGHEHYEEHLAGLRAALG"
     gene            2904..>3426
                     /gene="nadD"
                     /locus_tag="VF058_10770"
     CDS             2904..>3426
                     /gene="nadD"
                     /locus_tag="VF058_10770"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012183886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX6208823.1"
                     /translation="MGGTFDPIHQGHLVTAEEALWQFELDQVLFVPTGQPWMKHERGV
                     SPAEDRYLMTVIATSSNPRFRVSRLEIDRDGPTYSVDTLRSLRDEYGEGTELFFITGA
                     DAILQIFQWKDADQAFELAHFIAATRPGYDLSRFDQERAAEHPKITVMDIPALAISST
                     DIRERVAKGRPIRY"
BASE COUNT          560 a         1188 c         1240 g          438 t
ORIGIN      
        1 gccagatgta cgcggtgatc aagacgggcg ggaagcagca caaggtccgc cccggggacg
       61 tgatcgaggt cgagtacatg gacgtggatc cgggctcggc cgtggagttc gagccgatcc
      121 tcgtcatgga cgacaagaac aagccccatg tgggcaagtc gctggggaac gcgaaggtcg
      181 tggccaccct cgtcggcgac aagaaggccg ccaagatcaa ggtcgtgaag taccgggcca
      241 agagcggcta caagcggaac caggggcacc gccagcggat gaccgtcctg gagatccagg
      301 aggtgaagtt cggcggccgc gccgggaaga aggacgagga ggagccgaag gcagcggcca
      361 agaaggccgc cgccaagaag tcgacgagga agaaggccgc cgccaagaag tcgtccgcca
      421 agaagacctc ggccaagaag tcgtcgaaga agagcgcctc caagaagtcc tcgagccgga
      481 agaaggccac ctaggctctg ctatcctggg gccatggcac acaagaaggg cgccgctgcg
      541 tccaggaacg gccgcgacag cacctcgaag cggctcggcg tgaaggcctt cggcggagag
      601 accgtgaagg ccggcgagat catcgtgcgc cagcggggca ccaaggtgca cccggggctg
      661 aacgtgcgcc ggggcggcga cgacacgctc ttcgcggtgg ccccgggcag cgtgcgcttc
      721 tactcctcga acggccgcaa gtacgtcacc atcgaggcct gagagcctcc tcccactcct
      781 cgatcagggg cttcagacga cggttcgccg ccctgatccg ccccatgtcc ccgacccggc
      841 gcatccctcg gatcccgacg atcccgaaga ggctcggcac cggctcggga tcgggacgct
      901 cgggcggacg ggcgaagaac aggaacgcca tccgccgttc gccctctcgc cggtagcgga
      961 tctcgaggac aggcgtcccc cgctgtcgcc gggctcgctc cacctccccg aagcggacct
     1021 cctcgccgtc gtccgaccgg aaggtggggg tctctccctc gagcgagagt gtgccccggc
     1081 gctccttcga ctccccgggg cgccacagcc aggcgatcgt ccgcttgggc acggcaccta
     1141 tactcgcacc gcatgtccga gttcgtggac gaggccgtgg tctacgcgcg cggcggccgc
     1201 gggggcgacg gcgccgcgtc gttccaccgc gagaagtacc ggcccaaggg cggccccgac
     1261 gggggcgacg gcggccgggg aggcgacgtg atcctggagg cctctcccga cgtgttcgac
     1321 ctggcctcgg tcgcccgcca cccgcatcag cgggccgagg acggcggcca cgggcgcggc
     1381 gacaatcggc aaggcgccga tggcgacgac ctcgtgatcc cggtccccga cggcgtcttc
     1441 gtcgaggagg agcgcggccc ggtggcggat ctggtggggc ccggggctcg cgtggtggtg
     1501 gcccgcggcg gtcgcggcgg gcgagggaac gcctcgctgg cctcggggcg caaccgcgcg
     1561 ccccagacgg cggagcgggg cgagccgggg gaggagcgcc ggctccgcct ggagctccgg
     1621 ctcgtggccg acgtgggcct ggtcgggctt cccaatgcgg gcaagtccac gctgttgagc
     1681 gccttgaccg cagcgaggcc caaggtggcc gactacccgt tcacgacgct cacccccaac
     1741 ctcggcgtgg ccgaggggga gcggcgcgtc gtgctggcgg acgtcccggg attgatcgag
     1801 ggggcgcacg agggccgggg cctcggccac cggttcctgc gccacgtctc ccggtctcga
     1861 gcgctggtct tcgtcgtgga cgtcgcgggc gaggatcccg cggccgacct ccggctggtg
     1921 cgggccgagg tggcggccta cgatcccgag ctccccgagc ggccctggct cgtcgctgcg
     1981 acgaaggcgg acctgctgga ggcggaggag gccgagcgcc gggccgaggc gctccgcgag
     2041 gagggcgaga cgcacctcgt gtcgggggcg acgggggagg gcctcgaccg cctcgcggag
     2101 cgcctcggcc gcctggcaga cgagagcacg gtcgagcgca agcccttcgt cgttctccga
     2161 cccggccggg agcccttcac cgtccgccgc gagggtgacg gcttccgggt cgaggggcgc
     2221 atcgtcgagc gctgggtcga ggaggccaac ctcgatgacc cggcccaggt cgtcgcgctc
     2281 caggaccgcc tccgccgaga aggcgtggag cggaagctcg aggagctggg tgcccgtcgc
     2341 ggggacgagg tccggatcgg ggaccaggcc ttcgagtacc accccgggta gcatccggtc
     2401 atgaccaagg acgagctctt ccgcctggag caggacgcct gggttcccct ccacgacctc
     2461 gtgcacggcc tcgacccgga gtccgcggag cgtcccttga cggacctctg gaccgtcagg
     2521 gacggcctgg cccacatcgc ctgctggcag gccgaggccg cgcagatgct gctccagatc
     2581 cgagagggca cctacgaggg gtgggatgga gacgaggacg aggtcaacgc gcggttccac
     2641 gaggcgacga aggatctccc gctcgacgag gtgatggcga gcctccacgc gtcccgctcc
     2701 cggatgctcg aggagctcga ccgcttgccc gacgacctgc tcgacgacga ggcccgctcc
     2761 tggttcgtgg agagcggcca cgagcactac gaggagcacc tcgctgggct cagggccgcc
     2821 ctcggatagc cggcgaaatc cacgggaaac cgcaggtccg gtcatacaat cgccgcgtgg
     2881 acgcccccaa ccggctcggc gtcatgggcg gcaccttcga cccgatccac cagggccacc
     2941 tcgtcaccgc cgaggaggcg ctctggcagt tcgagctgga tcaggtcctc ttcgtgccga
     3001 ccggccagcc gtggatgaag cacgagcgag gggtctcgcc cgccgaggac cgctacctga
     3061 tgaccgtgat cgcgacctcc agcaacccga ggttccgggt ctcgcgcctc gagatcgacc
     3121 gcgacggccc cacctactcc gtggacaccc tccgctcgct tcgggacgag tacggggagg
     3181 ggaccgagct cttcttcatc acgggggccg acgccatcct ccagatcttc cagtggaagg
     3241 acgccgacca ggcgttcgag ctggcccact tcatcgcggc gacgcggccc ggctacgacc
     3301 tctcgcgatt cgatcaagaa agggccgccg agcatccgaa gatcactgtg atggacatcc
     3361 cggccctggc gatctcctcc acggacatcc gggagcgcgt cgccaagggc aggcccatcc
     3421 ggtacc
//
LOCUS       DASUTW010000312         9083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1788646, whole genome shotgun sequence.
ACCESSION   DASUTW010000312 DASUTW010000000
VERSION     DASUTW010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9083
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788646"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VF058_10775"
     CDS             <1..612
                     /locus_tag="VF058_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208824.1"
                     /translation="AGPAAAATRNVGVLLALPLVIEGYQQWRADRSRAPLLGGIAAAA
                     AIGLAVGAYLLFWKLKVGDWWAPLTQQVNWERQRVFPLESLWLGTREALRWIGVYPGG
                     YHLLDWLLVIPALAAAGWVALRARPSYAVYAWSSLLVPLSFTFLPRPFLSMPRFLLVI
                     FPLVWAPAVWAERRRGVHEVVLVAFSLGLGLMTVLFVTWYFVF"
     gene            675..1313
                     /locus_tag="VF058_10780"
     CDS             675..1313
                     /locus_tag="VF058_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6208825.1"
                     /translation="MPVTVVIADDHQLVRQGLRRYLETAEDISVVGEASGGVEAVEVV
                     GKLQPDVVVLDVRMDDLDGIEATKRIRESSPDTGVVILTGHEEERVVLEAVKAGARGY
                     VLKARDAEHLVNTVRLVADGNMVIDPDAVGAVAERLDGETGRVGGLGDLTKRELEVLR
                     LLAMGHTNRKIAEQLFISPDTVKTHLEHIYQKLGAPDRTAAVAEALRRQLID"
     gene            complement(1310..3577)
                     /locus_tag="VF058_10785"
     CDS             complement(1310..3577)
                     /locus_tag="VF058_10785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX6208826.1"
                     /translation="MAIRTIPRKPSRKHAPRKGTSTRSLKRGSRRQRQRRRGPVRRWI
                     RKWWWLWAPPLLLGLVGVGALVYLYVTIPLPPERVLAQTTFLFDRDGNRLTTLDGPED
                     RSIVELDRVPQHVRQAVLAAEDSRFYDHPGISVVDIIRAGWVDLTGGRTTGASTVTQQ
                     LVRNVFPEVGTERTITRKVKEAILALKLERELAKNEILERYLNTIYLGDGTYGFQAAS
                     RHYFGKPVAGINVLQAATLAGMIARPEGFNPRVNPGNAMVRRNFVLDRMAELGYIPRA
                     RAEKLKQRPVKTRKLEEGFADSPAAWFIDYTRQWLTNKRLSPLENPREQTYQGGLRIR
                     STLDMRWQQAAINAIRNRYSYDGAPDVALVAIDPQTGEIRAVASSDAYSADPEERVFH
                     PATFGCSVSRGCGWKVDTNGDGQKERQSATGRQTGSAFKMFTVGTAVEQGVSLRTTFS
                     GASPVRLDEPPCGSEADPWEPENFGGSSYGSMDLYGATASSVNTIFAQLISHVGPNNV
                     VEFAHRMGINSKVPEFCSITLGAVEVNVLEMTSAFATLAGGGVRHEPTPVQAIRSPEG
                     EVLWRNRGKGEQVMDENDAWQTVDALERVMYGTAAGANFGVPAFGKTGSEDDSTDGWF
                     CGSTSELTACVWVGHHEGQIPIPGLTGGGWAAPVWRDFMVAAHEDLEPKPFPTPELTG
                     EIVQPEVPLPAATTSPSPEPEEEERKPKPKPTQPPEPPPSPEPEPTEPPSPSPSPTQA
                     TNPGSGGRSSDPPDP"
     gene            complement(3623..5215)
                     /locus_tag="VF058_10790"
     CDS             complement(3623..5215)
                     /locus_tag="VF058_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208827.1"
                     /translation="MTQALVPAAILGPAAAALVWLALVRWVWPRRLVAGAVWIGSQAA
                     AWAVFWEVFGGEQPAWMGLGPDLLRATLAAAAGAAVTLVLPRIELLAPRASSAGMAAA
                     GVGLAAVVAAAYSSSLVALAVIALLPTIAVGLAGAVGDRPRGAPGLLGFAAADAAVVG
                     GLLIVLERSGTTGLEPVGSLDLGVALVLLGAAVKLGAVPHVGTWRASAGEGIVSPLAV
                     VLRGQGAVLAAVAAFTLARVSPSAPLAAVSAGLAVLAGLAGLAARSPGGSLAAVGGAG
                     AAAALVALGLGGGVGARAALVLLPVFVLASGVVAASGWPGVGDPRDPRDPRERRRAWR
                     WVAALALAIGAASLVGLPPAGGFPGTWLALSLASARAVSEPWHLALVVGLASGLALAG
                     IGAIAALRSAGTRPLPAALSLLGGLALLYAGAQPMRLGLGWLIRVERELGIGEVLPSA
                     GAPALPAIGGVDLAWAVAPALLIVGAGLVLPSPAPPRFARRAAGLARRLRLGRALAAL
                     LEGAAAAGAAWIVLRGVDLGFL"
     gene            complement(5212..6069)
                     /locus_tag="VF058_10795"
     CDS             complement(5212..6069)
                     /locus_tag="VF058_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208828.1"
                     /translation="MAALGIPLALLLGAAADLVASFVRSEARLRPVLAFRAELRRALT
                     RREPTSLLAGLGAGAALLGAGLITAAAIGASPGDPGLLMLALALAVVGSHLAASERVT
                     RPAAARARARALESALVYAGAAAGLGALFLRWRADDLTAVWGAGEILGFPLAVGPAAA
                     AIGQALALLAVAAAAGLLTPLETEPPRGRHRRPAAAALLLRMGRWGLAGALAALVPAF
                     AVGFDLVEGGDAPAFARWAGVALVASAVVGAIWGALDRLGPRLRLRASVPALLAASAA
                     AVVLVSVGR"
     gene            complement(6083..6457)
                     /locus_tag="VF058_10800"
     CDS             complement(6083..6457)
                     /locus_tag="VF058_10800"
                     /inference="COORDINATES: protein motif:HMM:NF036456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HEX6208829.1"
                     /translation="MSREPEGFGTVDYTLAKRLLLRDFGRGLLSRLDICDAHPELMRA
                     ARNVGEDMGRPCPVCDEANLVRLAYVFSDELKADNGRVWPFREALRVAARYLDGACYV
                     VEVCTECSWNHLSEAFVARSAG"
     gene            6551..7075
                     /locus_tag="VF058_10805"
     CDS             6551..7075
                     /locus_tag="VF058_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208830.1"
                     /translation="MTDIKETQEEIRAWFAGRIPDGWFTEDPEINVDRDEILVVGRIE
                     PPEIEKGASEEARREAELGRIARFREETRDKRIGVALGAERRFRTKVSWGAECGDTKR
                     LFTTLSVPVMTRLRMPERAVLDTLVDAGVARSRSHALAWCVRLVARHQGEWLEELRSA
                     FSEVERVRAEGPSL"
     gene            7072..8034
                     /locus_tag="VF058_10810"
     CDS             7072..8034
                     /locus_tag="VF058_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020521685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX6208831.1"
                     /translation="MSDTLEIREAAALDAYSRVVTSVAETLTPSVASLRMKRRVRGGR
                     RAEGAGSAVVMAPDGYLLTSAHVVAAADGGTAVFVDGRSLDLEVVGTDPLSDLAVIRV
                     AADDLPAATLGDADDLRVGQLVVAVGNPMGFAGSVTAGVVSALGRSLPTRTAAAGRLV
                     ENVIQTDAALNPGNSGGALSDGESHVVGINTAVAGVGLGLAVPINDTTRGIIGALLRE
                     GRFRRSYLGITGGWRPLPPRVAERLGRHAGVEVVEVVPGSPADRAGLRPEDLILDVGG
                     ESVTDVGDLQRLMVAELIDADVRVRVFRDGDVVEVSVRPVELQT"
     gene            complement(8040..>9083)
                     /locus_tag="VF058_10815"
     CDS             complement(8040..>9083)
                     /locus_tag="VF058_10815"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEX6208832.1"
                     /translation="PISLVIAALLWGGVGATALSIFGNTGWALVVADWFGPEVARSWT
                     AALTAPWVEEIFKALGIVLIYLIARREIDDVMDGFVYGAMVGLGFTLVEDVFYFLGAF
                     GGEVEGVLTGFYLRVIASGFYGHVLYSALAGMGIAYFVTRKGVVSEARRWAVPTGLFL
                     IAVLAHFLWNSPLLDLFPEEPWEGTDWLVILLATAVKGLPLLVFVVLMVRLAQRREHR
                     WLRAALETEVGRPGLTREELEALDHPVDRRRARREAAARGGPRAAKAMKKLQREQVNL
                     AMVATRTHDPDHPDVLRQHVYCAELRAWVRRLMASSEGEGARPSGGPPPGEAPPPPPP
                     GATPPGSPPPPST"
BASE COUNT         1277 a         3397 c         3319 g         1090 t
ORIGIN      
        1 gctggccccg ccgcggccgc gacgcgcaac gtcggcgtcc tgctggcgct ccccctcgtc
       61 atcgagggct accagcagtg gcgggcggat cggtcgaggg cgccgctcct cggggggatc
      121 gcggcggccg ccgcgatcgg gctcgcggtc ggggcctacc tcctcttctg gaagctgaag
      181 gtgggcgact ggtgggcgcc gctcacccag caggtgaact gggagcgcca gcgcgtcttc
      241 ccgctcgagt cgctgtggct agggacccgg gaagcgcttc ggtggatcgg tgtctacccg
      301 ggcgggtacc acctcctgga ctggctcctg gtgatccccg ccctggccgc cgcgggctgg
      361 gtggcgctcc gagcccgccc ctcctacgcc gtctacgcct ggagcagcct cctcgtgccg
      421 ctctcgttca ccttcctgcc tcgcccgttc ctctcgatgc cgcggttcct gctcgtgatc
      481 ttccccctgg tgtgggcacc ggcggtctgg gccgagcgcc gccgaggggt gcacgaggtg
      541 gtgctcgtgg cgttctcgct cgggctgggg ctgatgaccg tcctcttcgt cacctggtac
      601 ttcgtcttct gatttccccg cggcccggga ggtcgctacg atacgcgcag ccgaacagtc
      661 ggggggaagg ccgcgtgccc gtcaccgtcg tgatcgccga cgatcatcag ctcgtcaggc
      721 agggcctgcg ccgctacctc gagaccgcgg aggacatcag cgtcgtgggc gaggcctcgg
      781 gcggcgtcga ggcggtcgag gtcgtgggaa agctgcagcc ggacgtggtc gtgctcgacg
      841 tgcgcatgga cgacctcgac ggcatcgagg ccacgaagcg gatccgagaa tcgagccccg
      901 acaccggcgt ggtgatcctc acgggacacg aggaggagcg ggtcgtcctc gaggcggtca
      961 aggccggcgc ccgaggctac gtcctgaaag ctcgcgacgc ggagcacctc gtcaacacgg
     1021 tgcgcctcgt ggccgacggc aacatggtca tcgaccccga cgcggtgggg gcggtggccg
     1081 agcggctcga cggggagacc ggacgcgtcg gcggcctggg cgacctgacg aagcgggagc
     1141 tcgaggtcct ccgtctcctg gcgatgggtc acaccaaccg caagatcgcg gagcagctct
     1201 tcatctcacc cgacacggtg aagacccacc tcgagcacat ctaccagaag ctcggggctc
     1261 cggacaggac ggcggcggtg gccgaggcgc tgcgtcgcca gctcatcgac tagggatccg
     1321 gaggatcact cgatcggcct ccgctcccgg ggttcgtcgc ctgggtggga gagggcgacg
     1381 gcgagggagg ctcggtcggt tcgggctccg gggagggcgg aggctccggc ggctgcgtcg
     1441 gcttcggctt cggcttcctc tcctcctcct ccggctcggg cgatggcgag gtggtcgcgg
     1501 cggggagggg gacctccggc tgaacgatct cgcccgtgag ctccggggtg gggaacggct
     1561 tcggctcgag gtcctcgtgc gcggcgacca tgaagtcgcg ccagaccggc gcggcccagc
     1621 caccgcccgt caggccgggg atggggatct ggccctcgtg gtggccgacc cagacgcacg
     1681 cggtcagctc gctcgtcgag ccgcagaacc agccgtcggt cgagtcgtcc tcggatcccg
     1741 tcttcccgaa cgccgggacg ccgaagttgg cgcccgccgc cgttccgtac atcacgcgct
     1801 cgagggcgtc caccgtctgc caggcgtcgt tctcgtccat cacctgctcg cccttgccgc
     1861 ggttcctcca gaggacctcg ccctccgggg agcggatcgc ctggacgggc gtgggctcgt
     1921 ggcgaacgcc gccgcccgca agcgtggcga aggcggaggt catctccagg acgttgacct
     1981 cgacggcacc gagggtgatg gagcagaact cggggacctt gctgttgatc cccatacggt
     2041 gagcgaactc caccacgttg ttcgggccca cgtgggagat cagctgggcg aagatcgtgt
     2101 tcaccgagga cgccgtggcg ccgtagaggt ccatcgaccc gtagctcgag ccgccgaagt
     2161 tctccggctc ccacgggtcg gcctccgagc cgcacggcgg ctcgtccagc cggaccgggc
     2221 tcgcgccaga gaacgtcgtg cggaggctca cgccctgctc caccgccgtc ccgaccgtga
     2281 acatcttgaa cgcggacccc gtctgacggc cggtggccga ctgccgctcc ttctggccgt
     2341 ccccgttcgt atccaccttc cagccgcagc cgcgggagac gctgcagccg aacgtcgcag
     2401 ggtggaacac acgctcctcg gggtcggccg agtaggcgtc actcgaggcc acggctcgga
     2461 tctcgccggt ctggggatcg atcgccacca gcgcgacgtc gggggcgccg tcgtacgagt
     2521 agcggttcct gatcgcgttg atcgccgcct gctgccaccg catgtcgagc gtggacctga
     2581 tccgcagccc gccctggtag gtctgctccc tcgggttctc cagcggggag agcctcttgt
     2641 tcgtgagcca ctgccgcgtg tagtcgatga accaagcggc ggggctgtcg gcgaacccct
     2701 cctcgagctt cctggtcttg accggccgct gcttcagctt ctcggcgcgg gcccggggga
     2761 tgtagcccag ctccgccatc cggtccagga cgaagttcct ccggaccatc gcgttgccgg
     2821 ggttgaccct ggggttgaag ccctcggggc gggcgatcat gccggccagc gtcgccgcct
     2881 gcagcacgtt gatgcccgcc accggcttcc cgaagtagtg gcgcgacgcc gcctggaacc
     2941 cgtaggtgcc gtcacccagg tagatcgtgt tgaggtagcg ctcgaggatc tcgttcttcg
     3001 cgagctcccg ctcgagcttg agggcgagga tcgcctcctt caccttccgg gtgatcgtcc
     3061 gctccgtccc cacctcgggg aagacgttgc ggacgagctg ctgcgtgacc gtggaagccc
     3121 ccgtggtcct ccccccggtg aggtccaccc atcccgcgcg gatgatgtcc accaccgaga
     3181 tcccgggatg gtcgtagaag cgggagtcct ccgcggccag cacggcctgc cgcacgtgct
     3241 ggggcacgcg gtccagctcg acgatcgagc ggtcctccgg tccgtccagg gtggtgagcc
     3301 ggttcccgtc gcggtcgaac aggaaggtgg tctgggccag cacgcgctcg ggcggcaggg
     3361 ggatcgtgac gtagaggtag acgagcgccc cgacgccgac caggccgagc aggagcggcg
     3421 gcgcccagag ccaccaccac ttgcggatcc agcggcgcac ggggccccgc cggcgctgcc
     3481 gctggcgcct ggacccacgc ttcagcgatc gagtggacgt ccccttccgc ggggcgtgct
     3541 tcctcgaagg cttcctcggg atcgtccgga tggccatcgg agtcgagtct acccaccatg
     3601 ctccggtttc gaccccggat cgtcagagga accccaggtc gacgccgcgg agcacgatcc
     3661 aggccgcccc ggcggccgcc gcgccctcca ggagcgcggc gagcgcccgg cccaggcgca
     3721 gccggcgggc cagcccggcg gcccggcggg cgaaccgggg aggggccggg gaggggagca
     3781 cgagccccgc gcccacgatc aggagggccg gggccacggc ccaggccagg tccaccccgc
     3841 cgatcgccgg cagcgccggg gccccggccg agggcagcac ctccccgatc cccagctcgc
     3901 gctccacgcg gatgagccag ccgagcccga gccgcatcgg ctgggccccc gcgtagagga
     3961 gggccagccc tcccaggagg gagagcgcgg ccggcagagg acgtgtgccc gccgatcgga
     4021 gcgccgcgat cgctccgatg cccgcgaggg ccagccccga cgccagcccc accaccagcg
     4081 cgaggtgcca gggctccgag accgcccggg ccgaggccag cgagagcgcg agccacgtcc
     4141 cggggaagcc gccggcgggg ggcaggccca ccagcgaggc ggccccgatc gcgagggcca
     4201 gcgccgccac ccatcgccaa gcgcgccgcc gctcgcgggg atcgcggggg tctcgggggt
     4261 cgcccacgcc cggccacccg gaggccgcga cgacgcccga ggcgagcacg aagaccggga
     4321 gcaggacgag ggcggcgcgg gcgccgacgc ccccgcccag gccgagggcc acgagcgccg
     4381 cggcggcgcc cgcgccgccc accgccgcca gcgagcctcc cggggaccgg gcggcgagcc
     4441 cggccagtcc cgcgagcacg gccagcccgg ccgagacggc ggccagcggc gccgacggcg
     4501 agacccgcgc gagcgtgaag gcggcgacgg ccgcgagcac cgcgccctgg ccgcgcagga
     4561 ccaccgccag gggcgacacg atcccttcgc cggccgacgc cctccacgtt ccgacgtggg
     4621 gcaccgcccc cagcttgacg gccgccccca ggagcacgag cgcgaccccg aggtccaggg
     4681 agcccaccgg ctccagcccg gtcgtgccgg accgctcgag cacgatcagc agcccgccca
     4741 cgacggccgc gtccgccgcg gcgaaaccaa ggagccccgg cgccccccgg ggccgatccc
     4801 ccaccgcgcc ggccaggccc accgcgatcg tcggcagcag cgcgatgacg gccagcgcca
     4861 ccaggctcga ggagtaggcg gccgcgacca ccgccgccag tcccacgccg gccgcggcca
     4921 tcccagcgga cgaggcccgc ggcgccagga gctcgatccg gggaagcacg agcgtcaccg
     4981 cggccccggc ggccgccgcc agggtcgccc ggaggaggtc cggccccagg cccatccacg
     5041 cgggctgctc tcctccgaac acctcccaga acaccgccca cgccgcggcc tgcgacccga
     5101 tccagactgc gccggccacg agccgacggg gccagaccca cctgaccagc gccagccaga
     5161 cgagggcagc ggccgcggga cccaggatcg ccgcggggac cagggcctgc gtcatcgccc
     5221 caccgagacg agcacgacgg cggcggcgga cgcagccagc agcgccggga ccgacgcgcg
     5281 cagccgcagg cggggaccca gacgatccag cgccccccag atcgcgccca cgacggcgga
     5341 cgccaccagc gccaccccgg cccagcgcgc gaacgccggc gcgtcgccac cctcgacgag
     5401 gtcgaacccc acggcgaagg ccgggacgag ggccgcgagg gcgccggcca gcccccaccg
     5461 ccccatccgg aggaggaggg cggccgcggc ggggcggcgg tgccgtcccc gcgggggctc
     5521 ggtctccagg ggggtcagga gccccgccgc ggccgccacc gccagcaggg ccagcgcctg
     5581 cccgatcgcc gccgcggcag ggcccaccgc cagcgggaag cccaggatct cgcccgcgcc
     5641 ccagaccgcc gtcaggtcgt cggcccgcca cctgaggaag agcgccccga ggcccgcggc
     5701 cgcccccgcg tagacgagcg cgctctccag cgcccgggcc cgggcgcggg cggccgcggg
     5761 cctcgtcacc cgctcgctcg ccgccaggtg cgacccgacc accgccaggg ccagggccag
     5821 catcaggagg ccgggatcgc ccggggaggc cccgatcgcg gcggcggtga tgagccccgc
     5881 gcccagcagg gcggccccgg cgcccaggcc cgcgagcagg ctcgtcggct cccgcctcgt
     5941 cagggcgcgg cggagctcgg cccggaaggc caggaccgga cggaggcggg cctcggaccg
     6001 gacgaaggag gccacgaggt cggcggccgc gccgagcagg agggcgaggg ggatcccgag
     6061 ggccgccatc aggcccccgg cgctacccgg cgctgcgcgc gacgaaggcc tccgagaggt
     6121 ggttccagga gcactcggtg cagacctcga cgacgtaaca ggcgccgtcg aggtagcggg
     6181 ccgcgacgcg cagggcctcg cggaagggcc agacgcggcc gttgtcggcc ttcagctcgt
     6241 cggagaacac gtacgcgagc cgcaccaggt tggcctcgtc gcacacgggg cacggccggc
     6301 ccatgtcctc ccccacgttc ctggcggccc gcatcagctc ggggtgggcg tcgcagatgt
     6361 cgagccggga gagcagcccg cggccgaagt cccgcaggag gagccgcttg gccagcgtgt
     6421 agtcgaccgt cccgaagccc tccggctcac ggctcatggg ggcgattgta cggcacgccc
     6481 ccgacgcgac gcgccctcct ccccgcccgc gttgacgttt gccggatgac gatgtaatcc
     6541 tgtaatcacc atgaccgaca tcaaggagac tcaggaggag atccgggcct ggttcgcggg
     6601 gcggatcccc gacgggtggt tcaccgaaga cccggagatc aacgtcgatc gagacgagat
     6661 cctcgtggtt gggcgcatcg agccgcccga gatcgagaag ggcgcctcag aggaggcgag
     6721 gcgagaggcg gagctggggc gcatcgcccg gttccgcgag gagacccggg acaagcggat
     6781 cggcgtcgcg ctgggggccg agcgccgctt ccggacgaag gtctcctggg gcgccgagtg
     6841 cggagacacg aaacgcctct tcaccaccct cagcgtgccg gtcatgaccc ggctgcggat
     6901 gcccgagcgg gccgtgctcg acacgctggt ggacgccggg gtggcccgga gccggagcca
     6961 cgccctggcc tggtgcgtgc ggctggtggc ccggcaccag ggcgagtggc tcgaggagct
     7021 ccgatcggcc ttcagcgagg tcgagcgggt ccgcgcggag ggaccctcgc tgtgagcgac
     7081 acgctcgaga tccgcgaggc cgccgcgctc gacgcgtact cccgcgtcgt gacctcggtg
     7141 gccgagaccc tcaccccgtc ggtggccagc ctccggatga agcgccgcgt ccgcggcggg
     7201 aggcgcgccg agggcgccgg ctcggccgtc gtgatggccc ccgacggcta cctcctgacc
     7261 tcggcccacg tcgtggccgc cgcggacggg ggcaccgccg tcttcgtgga cgggcggtcc
     7321 ctcgacctcg aggtcgtggg caccgatccc ctctccgacc tggccgtaat ccgcgtggcc
     7381 gccgacgacc tcccggccgc gaccctcggg gacgccgacg acctccgggt gggccagctc
     7441 gtggtcgccg tggggaaccc gatgggcttc gcgggcagcg tcacggccgg tgtcgtcagc
     7501 gcgctggggc ggtccctccc cacgaggacg gccgcggccg gtcggctggt cgagaacgtg
     7561 atccagaccg acgccgccct caatcccggg aactcggggg gcgcgctctc agacggcgag
     7621 tcccacgtcg tgggcatcaa caccgctgtg gccggggtgg ggctggggct ggccgtgccc
     7681 atcaacgaca cgacccgcgg gatcatcggc gctctgctcc gcgagggccg gttccgtcgc
     7741 tcctacctcg ggatcaccgg cgggtggagg ccgcttccgc cccgcgtggc cgagcggctg
     7801 gggcgccatg ccggcgtgga ggtcgtggag gtcgtgccgg gcagcccggc ggatagagcg
     7861 gggctccgcc ccgaggacct catcctcgac gtggggggcg agtccgtgac cgacgtcggc
     7921 gacctccagc gcctcatggt ggccgagctg atcgacgccg atgtccgggt gcgcgtgttc
     7981 cgcgacgggg acgtcgtcga ggtctcggtc cgacccgtcg agctccagac ctgaggccgt
     8041 caggtggacg gaggcggggg cgagcccggc ggcgtcgcgc cgggcggcgg gggaggcggc
     8101 gcctcgccgg gaggcggccc tccggaagga cgcgccccct cgccttcgga gctcgccatc
     8161 agacgacgaa cccacgcccg gagctccgcg cagtagacgt gctgacgcaa gacgtcggga
     8221 tggtcggggt cgtgggtgcg cgtcgcgacc atcgccaggt tgacctgctc ccgctggagc
     8281 ttcttcatgg ccttcgccgc gcgcgggccc ccgcgagcgg cggcctcgcg gcgcgcccgc
     8341 cgccgatcga cggggtggtc gagcgcctcg agctcctccc gggtcagccc cggcctcccc
     8401 acctcggtct ccagcgcggc ccggagccag cgatgctccc gccgctgggc gaggcgcacc
     8461 atcagcacca cgaagaccag gagcggcagc cccttcacgg cggtggcgag gaggatcacg
     8521 agccagtccg tgccctccca cggctcctcg gggaagaggt cgaggagcgg cgagttccac
     8581 aggaagtggg ccagcacggc gatcaggaag agtccggtcg ggacggccca ccgcctggcc
     8641 tccgacacga ccccctttcg cgtgacgaag taggcgatcc ccatcccggc cagcgccgag
     8701 tacagcacgt gcccgtagaa gccgctggcg atcacgcgga ggtagaaacc cgtcagcacg
     8761 ccctccacct cgccgccgaa cgcccccagg aagtagaaga cgtcctcgac cagcgtgaag
     8821 cccagcccca ccatcgcgcc gtagacgaac ccgtccatga cgtcgtcgat ctcccggcgc
     8881 gcgatcaggt agatcagcac gatccccagc gccttgaaga tctcctccac ccacggcgcg
     8941 gtaagcgcgg ccgtccagct gcgcgcgacc tcggggccga accagtccgc gacgaccagg
     9001 gcccatcccg tgttgccgaa gatcgagagg gcggtggccc cgacacctcc ccacaggagc
     9061 gcggcgatca cgagggagat cgg
//
LOCUS       DASUTW010000313         3588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1789486, whole genome shotgun sequence.
ACCESSION   DASUTW010000313 DASUTW010000000
VERSION     DASUTW010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789486"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VF058_10820"
     CDS             <1..585
                     /locus_tag="VF058_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6208833.1"
                     /translation="EIVEEAHDGLDAVAKAERAVPDVALIDASLINCDGIRATALIRE
                     RVPDCRVILLTEDEDPATLVAAMEAGASGYLTKMAPLDHLAKSVRAVDRGETAIPRSM
                     VGSLIWRLLKRRDDQDEALGLVSRLTLREREVLALLADGANNDVIAQELVISPQTART
                     HVHNVLQKLNVHSRLEAVAMVRRTGVLKDSRLAP"
     gene            complement(589..1347)
                     /locus_tag="VF058_10825"
     CDS             complement(589..1347)
                     /locus_tag="VF058_10825"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6208834.1"
                     /translation="MRRRNARVPRAWSESGKRDPRMPGSLGIDAPEETTHLRRKIYAD
                     LRRRLEGAVGRASSVRSFPAGCVEKLRRDGVVTPLGCHEDVSLPLAHRDPESARFDRL
                     AGAALRRARQAVGLTLHEVSERSGGRFKPSTVAGYERGERSISLGRFAQLATVYDRPP
                     DQLLGEVLEGIYPSLRGGVAIDESRLAFVEEPAREAMRWLLRRVRESRFSAESPVLTI
                     RAGDLRVAILRSGEEPGTVLRKLRPAMVESGGRQ"
     gene            1784..2296
                     /locus_tag="VF058_10830"
     CDS             1784..2296
                     /locus_tag="VF058_10830"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX6208835.1"
                     /translation="MGPFPVAGEATWSNDWHAARCNPDPHLHKGIDIFAPAGTPLVAT
                     RNGIVTQKGIGAVSGLHVEITGHRGIQYFYAHLSRFAPDLALGQRVSRGDVVGFVGTT
                     GNARGTSPHVHLEVQPGGVPVAPKPFVDRWIARAELEATRWVRSVLSTPTTPEAEPAA
                     EASRSAQPRW"
     gene            complement(2331..2651)
                     /locus_tag="VF058_10835"
     CDS             complement(2331..2651)
                     /locus_tag="VF058_10835"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6208836.1"
                     /translation="MHHLDRIVGSALRRAREEIGLSLKDVERLTSGRFKPSTVAGYER
                     GERSITVSRFVALARVYGARPSGLLSDATHAQEGREDLVVVVPEDAAEVRDLAFAQGV
                     SSEG"
     gene            complement(2737..3219)
                     /locus_tag="VF058_10840"
     CDS             complement(2737..3219)
                     /locus_tag="VF058_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016472619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX6208837.1"
                     /translation="MSDRKVSAVDRFREEAAARGLGQLDVREFPEGTRTAADAARAIG
                     CEIGQIVKSVVLMGDGVPVVALTSGANRADTAKIAAIVGAARVRKADAVEAREATGFA
                     IGGTPPFSHPRTLRVLMDRDLLGYDEVWAAAGTPASVFPISPGDLVRAAGAEVVDLKE
                     "
     gene            complement(3216..>3588)
                     /locus_tag="VF058_10845"
     CDS             complement(3216..>3588)
                     /locus_tag="VF058_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013942019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX6208838.1"
                     /translation="AGAAGGMGGGLIAFLGARLRPGFELVAEALDLPRHVAGSDLVIT
                     GEGRFDRQSLSGKAPAGVLRLAREAGTRAFVVAGQVEEGVEIPAERVVDLSARAGLEG
                     AMDRPRTALADAAADLAEEVA"
BASE COUNT          538 a         1374 c         1200 g          476 t
ORIGIN      
        1 gagatcgtcg aggaggcgca cgacggcctc gacgcggtgg ccaaggcgga gcgggccgtc
       61 ccggacgtcg cgctgatcga cgccagcctg atcaactgcg atgggatccg ggcgacggcg
      121 ctgatccgcg agcgggtccc agactgtcgg gtgatcctcc tgaccgagga cgaggacccg
      181 gccacgctgg tggccgctat ggaggccggc gcgagcgggt acctcacgaa gatggcccca
      241 cttgaccacc tcgcgaagag cgtgcgggca gtcgaccggg gtgagaccgc gatccctcgg
      301 agcatggtgg gctccctgat ctggcggctc ctcaaacgga gggacgacca ggacgaggcg
      361 ctcgggctcg tctcccggct aacgctgcgg gagcgtgagg tcctggccct cctcgccgac
      421 ggcgcgaaca acgacgtgat cgcccaggag ctggtgatca gcccccagac ggcccgcacc
      481 cacgtgcaca acgtgctcca gaagctgaac gtccactcgc ggctcgaggc ggtggccatg
      541 gtccggcgca ccggcgtcct gaaggactca cggctcgccc cctgagccct actgccggcc
      601 gcccgattcc accatcgcgg ggcgcagctt tcgcaggacc gtccccggct cctctccgga
      661 acggaggatc gctacccgaa ggtcgcccgc ccggatcgtg agcacggggc tctccgcgga
      721 aaaccggctc tcccgcaccc gccgaagcag ccacctcatg gcctcgcgag cgggctcctc
      781 cacgaaagcc agcctggact cgtcgatcgc gacgccgccc cggagcgacg ggtagatgcc
      841 ctccaggacc tcgccgagga gctgatccgg tggccggtcg taaaccgtcg ccagctgcgc
      901 gaatcggccg agggagatcg atcgctcccc tcgctcgtat cccgcgaccg tcgaaggctt
      961 gaaccggccc cccgatcgct cgctgacctc gtgcagcgta agccccaccg cctggcgagc
     1021 cctcctgagg gccgccccag ccaggcgatc gaatcgagcc gactctgggt ccctgtgagc
     1081 caacggtagt gacacatcct catggcatcc gaggggcgtg accacgccgt cccgccgtaa
     1141 cttctctacg cagccagccg ggaacgagcg tacggacgag gcacgcccga cagctccttc
     1201 caaccgcctg cggagatctg cgtagatttt tcgccgcaga tgcgtggtct cctcgggggc
     1261 atcgattcct agactgccgg gcatgcgcgg atctcgcttc cccgattcgc tccacgcgcg
     1321 ggggactcga gcattcctcc ttctcatggt ggtagggggg cttctgtggc aggcctgggc
     1381 tccggcctgg cccgcggagc ccggaaaagg gccgccggaa tcgccgccgg gccagtcgaa
     1441 ccggcccgcg cccgaggagc ccccgccacc cgcgcccgag cctgagccgt cgccccagcc
     1501 ggagccctcc ccgtcgcctc agccgcagcc gtcgccccag ccggagcccg agcagggcga
     1561 ctccaagggc gacgccggaa aggcgccggc agccacaacg actgcggccg ggaatgcccg
     1621 cgggaatgcc cgtaagggcg ggcccacggg atgtgacggt gcgctggccc tcgaagccca
     1681 gcgacccgat ctcggcccgc cgggcagcac ggatcgtctc gccaggatcg tggcgccgct
     1741 ccagggccgg ggcatctccc tcttacaggc gtttcgccgc gtcgtgggcc ccttccccgt
     1801 ggcgggggag gccacctggt cgaacgactg gcacgcggcg cgctgcaacc ccgacccgca
     1861 cctccacaag gggatagaca tcttcgctcc cgccggcacc ccgctcgtgg cgacaagaaa
     1921 tggcatcgtg acgcagaagg gcatcggagc agtgagcggc ctgcacgtcg agatcacggg
     1981 ccacaggggc atccagtatt tctacgcgca cctcagtcgg ttcgccccgg acctggcgct
     2041 cgggcagcgc gtctcgaggg gcgacgtcgt ggggttcgtg ggaacgaccg ggaacgccag
     2101 gggcacgtcg ccgcacgtgc acctcgaggt ccagcccggc ggcgtgccgg tggccccgaa
     2161 gcccttcgta gatcgctgga tcgcccgcgc ggagctggag gcgacgagat gggtccggtc
     2221 ggtcctcagc accccaacga cgccagaggc ggagccggcc gccgaagcct caaggtcggc
     2281 tcagcccagg tggtgatcca gggctcgcgg accccccgcg gctcggagac ctagccctca
     2341 ctcgaaacgc cctgcgcgaa ggccaggtcc ctgacctcgg cggcgtcctc cgggacgacg
     2401 acgaccaggt cctcgcgtcc ctcctgggcg tgcgttgcgt ctgacagcag tccggaagga
     2461 cgggctccgt agacgcgggc cagggccacg aacctcgaca ccgtgatcga gcgctcccct
     2521 cgctcgtacc cggccaccgt ggagggcttg aagcggccgc tcgtcaggcg ctccacgtcc
     2581 ttcagggaga ggccgatctc ctctcgagcc cgccgcaggg cggacccgac gattcggtcc
     2641 agatggtgca taccggtgat ggtagaggga ggatcggctc gcggggagtc gtgattccgc
     2701 cccaccatcg tcgggcggcc gggggatgcg gccgactcac tccttcaggt ccacaacctc
     2761 cgcgccggcg gcccgcacga ggtccccggg cgagatcggg aagaccgagg ccggcgtccc
     2821 ggccgccgcc cagacctcgt cgtagccgag caggtctcgg tccatcagca ccctgagcgt
     2881 cctggggtgg ctgaacggag gcgtcccacc gatggcgaac ccggtcgcct ctcgggcctc
     2941 cacggcgtcg gccttccgca cccgggcggc gccgacgatc gccgcgatct tcgccgtgtc
     3001 ggcccggttg gcgccggagg tgagggcgac caccggcacg ccgtccccca tgagcaccac
     3061 gctcttcacg atctggccga tctcgcagcc gatcgcccgc gccgcgtcgg ccgccgtcct
     3121 cgtcccctcg gggaactccc tgacgtcgag ctggcccagt cccctcgccg cggcctcctc
     3181 gcggaaccgg tccaccgccg acaccttgcg gtcgctcacg caacctcctc tgcaagatcg
     3241 gcagcggcgt ccgccagcgc cgtcctcggc cgatccatcg ccccctcgag cccggcgcgg
     3301 gccgacaggt ccacgacccg ctccgccggg atctccacac cctcctccac ctgacccgcc
     3361 accacgaagg cccgggtccc ggcctcccgg gccagcctga gcacgccggc cggcgccttc
     3421 ccggagagcg actgccggtc gaatcgtccc tcccccgtga tgacgaggtc cgatccggcc
     3481 acgtgtcgcg gcaggtccag ggcctccgcc accagctcga agccgggccg gaggcgggcg
     3541 cccaggaacg cgatcagccc gcctcccatg cctccggccg cgccggcc
//
LOCUS       DASUTW010000314         2203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1532451, whole genome shotgun sequence.
ACCESSION   DASUTW010000314 DASUTW010000000
VERSION     DASUTW010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532451"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..87)
                     /locus_tag="VF058_10850"
     CDS             complement(<1..87)
                     /locus_tag="VF058_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015575782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX6208839.1"
                     /translation="MRSVTYSMSVSLDGYIVGPDGDFDWTVPD"
     gene            complement(301..1122)
                     /locus_tag="VF058_10855"
     CDS             complement(301..1122)
                     /locus_tag="VF058_10855"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6208840.1"
                     /translation="MTEEHVLRNRAAWDGFSEEFVEPGRELWGAEEPVWGIWGVPESE
                     VGMLGDVEGLDAVELGCGTAYVSAWVARRGARPVGLDNSPKQLETARRFQEEFGLSFP
                     LVQGDAERTPFPDASFDLAVSEYGAAIWCDPYAWIPEAARILRPGGRLLFLGHSPLAM
                     LCSPEGDEVVPIGDRLIRDQFGKHRSSGRRRPNSRSRTARWSGCSAGLGSRSRTSSSS
                     RLPRAQRRATSGSRSSGRAAGRPTTSGRPASAARLVVPTDVVDIGESPLAWSGTA"
     gene            1176..1481
                     /locus_tag="VF058_10860"
     CDS             1176..1481
                     /locus_tag="VF058_10860"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6208841.1"
                     /translation="MSGGRLLREARRAAGLGQRELARRARIAQPTLSRIERGLASPRF
                     DTLDRLLRECGLALDLVRRPGAEVDRTLIRERLRLTPGERARLAAREWEATRVFRGR"
     gene            1478..1969
                     /locus_tag="VF058_10865"
     CDS             1478..1969
                     /locus_tag="VF058_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208842.1"
                     /translation="MSEPPFDPLAALRTLADHGVRFVLIGGYAAALRGSPMITGDLDI
                     CYARDEANLRALSDALRRLGARLRGIDEDVPFRLDAEPLKAGDRFTFSTNAGPLDCLG
                     TPSGTDGFADLEASATDEDLDGLVVRVASLDDLIRMKRAAGRPQDLIAVEWLSALRDE
                     LDR"
     gene            complement(1978..2157)
                     /locus_tag="VF058_10870"
     CDS             complement(1978..2157)
                     /locus_tag="VF058_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208843.1"
                     /translation="MRRRDENDLTDEEFLELWEQGEPVELAPPREAPSSTSDAEEASP
                     PTSGRRRAGEHHRDA"
BASE COUNT          313 a          800 c          768 g          322 t
ORIGIN      
        1 gtcgggcacc gtccagtcga agtcgccgtc cggcccgacg atgtatccgt caagtgagac
       61 gctcatcgaa taggtcacgc tgcgcatcag aagtcctcct cggcaacggg ttcgacagta
      121 cgaccgccgg acgccgcggg actcatcgcg gctcgcggga tccgggctag cttcttggtg
      181 tccatgtgca cccactcgcc cggccgttcc cggttcagtc gatgacgacc agccgcctcc
      241 ccttcggcgt ccaacgcgcc ctagcatgtg ccacgcgagc cccctcccca gttgagaccg
      301 tcaagcagtc ccagaccatg cgagggggct ctcgcctatg tcaacaacgt ccgtgggaac
      361 gacgagccta gccgcgcttg cgggccttcc agatgtcgtc ggtcggccag cggcgcgccc
      421 actcgagcgt gacccactcg tagcgcgtcg ttgcgccctc gggagcctgg agctcgagga
      481 ggtcctcgac ctcgaaccca gaccggcgga gcaaccggac catctcgccg tgcgggatct
      541 ggaattcggt ctcctccgac cactcgagcg gtgcttgccg aactggtccc ggatgagccg
      601 gtccccgatc gggacgacct cgtcgccctc gggggagcag agcatcgcga gcggcgagtg
      661 gccgaggaac agcaggcgcc cgccaggccg aaggatgcgg gccgcctcgg ggatccaggc
      721 gtacgggtcg caccagatcg cggcgccgta ctccgagacg gcgaggtcga agctggcgtc
      781 cgggaacggg gtccgctcgg cgtcgccctg gacgagcggg aatgagaggc cgaactcctc
      841 ctggaaacgc cgggccgtct cgagctgctt cggggagttg tccaggccca cgggcctcgc
      901 cccgcggcgg gcgacccagg cggagacgta ggccgtgccg cagccgagct ccaccgcgtc
      961 caggccctcg acgtctccga gcatgccgac ctcggactcg gggacgcccc agatgcccca
     1021 gaccggctcc tcggcccccc agagctctcg cccgggctcg acgaactcct cggagaaccc
     1081 gtcccaggcg gctcggttgc gaaggacgtg ctcctcggtc acggggaaac gatatcccat
     1141 gtccatatcg atagtgtttg cgctatcatc accccatgag cgggggacgt ctgctccggg
     1201 aagcacgacg cgcggccggg ctcggtcagc gcgagctcgc gcgccgggcg cggatcgcgc
     1261 agcccacgct ctcgcgcatc gagcgggggc tcgcctcgcc gcggttcgac acgctggacc
     1321 ggctcctgcg cgagtgcgga ctggcgctcg acctggttcg gcggccgggg gcggaggtcg
     1381 accgaacgct gatccgggag cgcctgcgtc tgaccccagg tgagcgggcg cgactggcgg
     1441 cccgcgagtg ggaagcgacc cgggtgttcc gcggacgatg agcgagccgc cgttcgatcc
     1501 gctcgccgcg ctccggacgc tcgccgacca cggcgtccgg ttcgtgctga tcggcggcta
     1561 cgcggcggcg ctgcgcggct cgcccatgat caccggtgac ctggacatct gctacgcgcg
     1621 tgacgaggcg aacctccgag cgctctcgga cgcgctccga cggctggggg cgaggctccg
     1681 aggtatcgac gaggacgtcc ccttccggct cgacgctgaa ccgctgaagg cgggcgaccg
     1741 cttcaccttc tcgacgaacg ccggcccgct cgactgcctc ggcaccccct cggggacgga
     1801 cggcttcgcc gacctggagg cgtcggcgac ggacgaggac ctcgacggcc tcgtcgtccg
     1861 cgtggcctcg ctcgatgacc tgatccggat gaagcgcgcc gccggccggc cgcaggacct
     1921 gatcgcggtc gagtggctct cggcgctccg cgacgagctc gaccgttgaa gggagggtca
     1981 ggcgtccctg tggtgttcgc ccgcccgacg gcggccggac gtaggcgggc tcgcctcttc
     2041 ggcgtcactg gtcgaagagg gggcctcgcg cgggggagcg agctcgacgg gctctccctg
     2101 ctcccacagc tccaggaact cctcgtcggt gaggtcgttc tcgtcccggc gcctcatggg
     2161 ctcggcctac gcatccaact ccgcgggcgc ctccgccagg gcc
//
LOCUS       DASUTW010000315         4299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1400726, whole genome shotgun sequence.
ACCESSION   DASUTW010000315 DASUTW010000000
VERSION     DASUTW010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400726"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VF058_10875"
     CDS             complement(<1..656)
                     /locus_tag="VF058_10875"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6208844.1"
                     /translation="MEAAFFDLDKTIISKSSSLALTRPLYRAGFVTRSQLLRGAYAQM
                     VYLLLGADEKRMDRAKEGMLALSKGWDRDELEALIRDVLEDVVDPFIYQEALDLMALH
                     RALGRKVFIVSSSPEEIVRPLANRLGVTDVVATRAEIEDGRYTGELEFYCYREQKGEA
                     IREVAEREGIDLSGSYAYSDSATDLPMLEAVGNPIAVNPDRDLRRIAEERGWQIRDFR
                     R"
     gene            765..1253
                     /locus_tag="VF058_10880"
     CDS             765..1253
                     /locus_tag="VF058_10880"
                     /inference="COORDINATES: protein motif:HMM:NF039927.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5946 family protein"
                     /protein_id="HEX6208845.1"
                     /translation="MSRCPGCGVELPQRAGPTHAYFNCSPECWATYGEVAGRVLQDAA
                     VAGRTHQLLVDAYAAQHPGGEHPDKSVAIHLVGLHLMLERGVPPARIPPLYHRMASRE
                     GPWPHLPPPQDLGSLTIVDVAGAETSEALAEAVPAWAQSVWSAWSDHHGEVARLPERW
                     LP"
     gene            complement(1257..1655)
                     /locus_tag="VF058_10885"
     CDS             complement(1257..1655)
                     /locus_tag="VF058_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016434290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX6208846.1"
                     /translation="MARPERHAVVAVVRRDGRFLLIRRGPDAAMPGFWAPPAGRIEPG
                     ESHRDAVVREVREEVGLEVTPVRKVWECPTHDGGFQLHWWLAEASAGQLRPEPGEVNE
                     ARWILPEEFGDLEPTFEDHRPFFEEILPTL"
     gene            complement(1667..2128)
                     /locus_tag="VF058_10890"
     CDS             complement(1667..2128)
                     /locus_tag="VF058_10890"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6208847.1"
                     /translation="MSRGKLADRWAARPQVGGARRLADLQEAIAAAPLFAEVPKRHLR
                     SLALLSRVSSYPGGAEIVRQGTEGDTFYVVLEGEARIVKGRRTIARMKPGDFFGELAI
                     FDGEPRAASVVADGAVLCLKLEGPEFRKLLLREPRLGLTIMTAMARRLRSS"
     gene            complement(2261..2530)
                     /locus_tag="VF058_10895"
     CDS             complement(2261..2530)
                     /locus_tag="VF058_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208848.1"
                     /translation="MRRVLTVVFAGALLLGPASPALADPHPSQGASSEGRNEAGFGGG
                     PHCHVLITNHTPFDSAPAFPSHTAHLASGIGHVFAADPNCDGLPG"
     gene            2701..2793
                     /locus_tag="VF058_10900"
     tRNA            2701..2793
                     /locus_tag="VF058_10900"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2736..2738,aa:Sec,seq:tca)
     gene            2999..3964
                     /locus_tag="VF058_10905"
     CDS             2999..3964
                     /locus_tag="VF058_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX6208849.1"
                     /translation="MVRIPTNPLALREDTLQDLRDEFLVRCHAKNLSPRTIEWYAKHS
                     ELFIEWCRGRGLTAPAQLTTTLLEEYLIEAREAGRTPNTVRGYAQTLKTLSRFGHKKG
                     LIPENITADFEMPKVPQVIIQTFSEDQLRALLRKPDTRRWLGIRDRAILLTFLDTLVR
                     VSELVGVNAEDVDLKARTMRVMGKGSKQRELPLGQTVTQALRRYQRLLEDVRPEDPFF
                     VSRYGGRITRKSVHEVVAKYGKAAGIEGVRCSPHTLRHTGAKRFILAGGDVFTLQKLL
                     GHTTLYMVRRYVELASVDIQAQHERYSPADSLLSRPRAMTEPRER"
     gene            complement(4058..>4299)
                     /locus_tag="VF058_10910"
     CDS             complement(4058..>4299)
                     /locus_tag="VF058_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208850.1"
                     /translation="LHVVNKEYVENWMEGMDVYHNPRALHPLDPDMLPGAAHHRLLDD
                     GQVESFVTQWQPLASLTSIMVLRGEPGAVDAEETG"
BASE COUNT          683 a         1530 c         1432 g          654 t
ORIGIN      
        1 cgccggaagt cccggatctg ccaccctcgc tcctcggcga tcctccgaag atcgcgatcg
       61 gggttgaccg cgatcgggtt gcccaccgcc tcgagcatcg ggaggtcggt cgccgagtcc
      121 gaatacgcgt aggacccgga gaggtcgatc ccctctcgct cggccacctc gcggatcgct
      181 tctcctttct gctcccggta gcagtagaac tcgagctccc cggtgtacct cccgtcctcg
      241 atctccgccc gggtcgcgac cacgtccgtg acccccaagc gattcgccag ggggcgcacg
      301 atctcctccg gcgacgacga aacgatgaac accttccgcc ccagggcgcg gtggagggcc
      361 atgaggtcga gggcctcctg gtagatgaag ggatcgacca cgtcctccag tacgtcccgg
      421 atcagcgcct ccagctcgtc gcgatcccat cccttggaga gggccagcat cccctccttg
      481 gctcgatcca tccgcttctc gtccgccccg agcagcaggt agaccatctg ggcataggcc
      541 ccccggagga gctgcgacct ggtcacgaac ccggcccggt agagcggcct ggtgagcgcg
      601 agagagctcg acttcgagat gatcgtcttg tcgaggtcga agaacgcggc ctccatcgac
      661 gccatggtat cgcccctctc ccggagtgct cggcgctatg cttcgccgat gcgcgcgcag
      721 gggagggcga ccgaggtccg gccgctcggc gccccccggg tccgttgagc cgctgccccg
      781 ggtgcggcgt cgagctgccc cagcgagccg gccccaccca tgcctacttc aactgctcgc
      841 ccgagtgctg ggcgacatac ggggaggtgg ccgggcgggt cctccaggac gcggccgttg
      901 ccgggaggac ccatcagctc ctcgtggacg cctacgcggc ccagcacccc ggtggcgagc
      961 accccgacaa gtccgtcgcg atccacctcg tcgggctcca cctgatgctg gagcgagggg
     1021 tcccgccggc ccggatcccg cccctctacc atcggatggc gagccgggag ggtccctggc
     1081 cccacctccc tcccccccag gacctgggtt cgctgacgat cgtggacgtc gctggggcgg
     1141 agacatccga ggcgctggcc gaggctgtcc ctgcctgggc ccagagcgtg tggtcggcgt
     1201 ggtcggatca ccacggcgag gtggcccggc tcccggagcg ctggctcccc tgacgctcag
     1261 agcgtcggga ggatctcctc gaagaacggg cggtgatcct cgaaagtcgg ctcgagatcg
     1321 ccgaactcct ccgggaggat ccaccgggcc tcgttcacct cccccggctc ggggcgaagc
     1381 tgcccggcgc tcgcctccgc cagccaccag tggagctgga agccaccgtc gtgggtgggg
     1441 cattcccaca ccttcctcac cggcgtgacc tcgaggccga cctcctcccg gacctctcgg
     1501 acgacggcgt cgcgatgcga ctcccccggc tcgatcctcc cggcgggcgg ggcccagaac
     1561 cccggcatgg ccgcgtcggg ccctcgacgg atcaggagga accgcccatc ccgtcggacg
     1621 accgccacca ccgcgtgtcg ctcgggccgg gccatgcggt gcggtctcag gagcttcgga
     1681 gccgcctagc catcgccgtc atgatcgtca ggccgagccg cggctcccgc agcagcagct
     1741 tgcggaactc cggtccctcg agcttcaggc agaggaccgc cccatccgca acgaccgagg
     1801 ccgcacgggg ctccccgtcg aagatggcca actctccgaa gaagtccccc ggcttcatgc
     1861 gtgcgatcgt gcgccgcccc ttcacgatcc gggcctcgcc ctccaagacc acgtagaagg
     1921 tgtctccctc ggtcccctga cggacgatct ccgcgcctcc ggggtacgaa gacacccggc
     1981 tgagcagcgc cagcgatcgg agatggcgct tcggcacttc ggcgaagagc ggtgccgccg
     2041 cgatcgcctc ctggaggtcg gcgagccttc gagcgccgcc cacctggggc ctggcggccc
     2101 atcggtccgc gagcttcccc ctggacatca ggccggcctc ctcatggcgg gcctcacctt
     2161 ggagcaggcc tccgagagtg tcaagcacga cgcgcccgga gacgcgagga gggccggccg
     2221 gtggggctcc cgctcgagag cgggagcccc accgccccga tcaacccggc agcccgtcgc
     2281 agttcgggtc ggcggcgaac acgtgcccga tcccggacgc gaggtgggcc gtgtgggagg
     2341 ggaaggcagg cgcgctgtcg aacggggtgt ggttcgtgat gaggacgtgg cagtgcggcc
     2401 ctcccccgaa accggcctcg ttgcgcccct cgctcgacgc cccctgggac gggtgtggat
     2461 cggcgagcgc cggacttgcc ggtcccagga gaagcgctcc cgcgaagacc accgtgagta
     2521 cccttcgcat cgaacctcct ttcgctcgga cgacccgggt ggtcgccctc ggatggacca
     2581 catccggcat tcgataggtc cacggtgacg gttcgctcag ccggatcccg cccgggccgc
     2641 ccacggctcc ggaggcaccc agggggcgct ccgcacgcgc ctccgcgtat cctcggagcc
     2701 ggaggcgtca gggttcctgg tgggcccccc ggtcttcaaa accggtgagg cggcgacgag
     2761 ctgcctggcg ggttcgattc ccgtccgcct ccgctactga ataggcgtcc tgacctgggg
     2821 cttcgaggtc gcagcggatc gtcggtcgcc gaaaagaagg agtcgatcgg ccgccggata
     2881 tccgctcaat cggacaagtc ttcgcgagtt ttcgcgaaca tagaaccgta caagtcttgc
     2941 ccggggacgt tcatctcgaa gacttgtccg ccgtcctccc agagcaagga ggacacccat
     3001 ggtcaggatc ccgacgaacc cgctggcgct gagggaggac acgcttcagg acctgcggga
     3061 cgagttcctg gtccgctgcc acgcgaagaa cctgagcccg cggacgatcg agtggtacgc
     3121 gaagcacagc gagctgttca tcgagtggtg ccggggtcgt gggttgacgg cgccggccca
     3181 gctcacgacc acgctgctcg aggagtacct gatcgaggcg cgggaggcgg gccggacgcc
     3241 caacacggtc cgcggctacg cgcaaaccct caagaccctg agccggttcg ggcacaagaa
     3301 ggggctgatc ccagagaaca tcaccgcgga cttcgagatg ccgaaggtgc cccaggtcat
     3361 catccaaacc ttctcggagg accagctccg ggcgctcctg aggaagccgg acacaaggcg
     3421 gtggctgggg atccgcgacc gggcgatcct gctgacgttc ctggacacgc tggtccgggt
     3481 gtcggagctc gtcggtgtga acgcggagga cgtcgacctg aaggcccgga cgatgcgggt
     3541 catgggcaag gggtcgaagc agcgggagct accgctgggt cagacggtca cccaggcact
     3601 gcggcgttac cagcggctgc tggaggacgt gaggccggag gaccccttct tcgtcagccg
     3661 ctacggcggg cggatcacca ggaagtcggt ccatgaggtg gtcgcgaagt acgggaaggc
     3721 agcggggatc gagggggtga ggtgctcccc gcacacactg cgtcacaccg gggccaagag
     3781 gttcatcctc gccggcggcg acgtcttcac gctccagaag ctcctcggcc acaccacgct
     3841 ctacatggtg cggcgctacg tggagctggc cagcgtcgac atccaagctc aacacgagcg
     3901 ctacagccca gccgactcgc tgctcagtcg gcctagagca atgaccgaac ctcgtgagcg
     3961 ctaacaagac cgcgggaagc cgtcaggcgc cctctctgtg tcgctcccct cccggagacc
     4021 tggcgcttgc ttacgagctc gcagcctgct gttgagccta cccggtctct tcagcgtcca
     4081 cggctcctgg ctcgccgcgc aggaccataa tcgaggtgag cgacgccagc ggctgccact
     4141 gcgtgacgaa cgactcgacc tgtccgtcgt cgagaagtcg atggtgcgcc gcacccggaa
     4201 gcatgtccgg atcgagcggg tgcagcgcgc gcgggttgtg gtagacgtcc attccctcca
     4261 tccaattctc cacgtactcc ttgttgacca catggagga
//
LOCUS       DASUTW010000316         9915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1662092, whole genome shotgun sequence.
ACCESSION   DASUTW010000316 DASUTW010000000
VERSION     DASUTW010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9915
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662092"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..332
                     /locus_tag="VF058_10915"
     CDS             117..332
                     /locus_tag="VF058_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208851.1"
                     /translation="MIELFGDLIRSHGWHVVAELLLLVLATVFTAFYVAALVRGRVRA
                     VECAGCGRIASRAHAECPRCGTSLPTS"
     gene            469..1518
                     /locus_tag="VF058_10920"
     CDS             469..1518
                     /locus_tag="VF058_10920"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX6208852.1"
                     /translation="MATTALLLAACQQGLPQSSLEPAGPRAERINDLSTIVFWMAGVV
                     FVLVEGAIIFITIRYRRRRREERAPVQIHGNNRLEILWTVIPALLLAGLAFPTVAAIF
                     DLSRRPPGGDVLEVTVIGHQWWWEFRYPDLNIVTANELVVPVDTPVHLSLESAETPGG
                     IAEFTADGQPVTQNAIPVIHSFWVPRLAGKQDLVPGKVETLTIEADETGTFLGQCTEY
                     CGTSHANMRKRVIVETEQGFQEWVSRMQAPPAEPEPGSLAAQGAEFFQNFGTGSCLAC
                     HGVEPGAGGTVGPNLAHLASRGTFGAGLFETNRENLQRWLDDPRAMKPGVVMPDYNLT
                     EEQIEAIAEYLLTLE"
     gene            1538..3430
                     /gene="ctaD"
                     /locus_tag="VF058_10925"
     CDS             1538..3430
                     /gene="ctaD"
                     /locus_tag="VF058_10925"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX6208853.1"
                     /translation="MAIAQPLPRGSVFRRPTATTGFVSWLTTVDHKRIGILYGVTAFA
                     FFLLGGLEALLIRWQLATPEGQLLDADVYNQLFTMHGITMIFLVVMPLSSAFFNYLVP
                     LMIGARDVAFPRLNAFSYWIFLLGGLFLYSSWFLGGAPDGGWFGYAPLSSQMPGNNLD
                     FYAVGLQIVGVASLVSAVNFIVTIFNERAPGMSLMRMPVFVWMTLVVSFLLLFALPVI
                     AIALFQLAGDRILGTHFFDAAAGGDPILWQHMFWIFGHPEVYILILPAMGIVSEILPT
                     FSRKPLFGYPAVVFAGAAIGFMGWGVWAHHMFTSGLGPVANSAFALSTMFIAVPTGIK
                     IFNWLGTMWGGAIRFTTPMLMSIGFVAMFVIGGLSGVTHSIVPADWQQHDTYYIVAHF
                     HYVLFGGAIFGLFGGAYYWFPKFTGRTYSEPLGHLHFWLMLIGFNLTFAPMHLLGLEG
                     MPRRIYTYGEGLGFDVWNMVSTIGAFTIAASVLVFMWNVVRSLSRGEKAPPDPWDGRT
                     LEWTIPSPPPHYNFAEVPQVRARDDFWHRKYTEDGSGRVQPVVAGAATEEAEEGHAEE
                     EHAEEEHLDIHMPDPSYWPLVAASGLPFIGYGLIYDWIWAVPGAVLLLFGLFGWSLEP
                     VAEEGH"
     gene            3430..4023
                     /locus_tag="VF058_10930"
     CDS             3430..4023
                     /locus_tag="VF058_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX6208854.1"
                     /translation="MAQAEVRHEAHTATGLDNRKLGMWAFLGSECLFFGALITTYLLY
                     RGRSVQGPFPEDIYDIPFTSVSSFVLLMSSLTMVLALSAIQRGDHQGLRIWLLATAFL
                     GMTFLGGQVYEFTVFVNEGLTVTRNVFGSAFFVLTGFHGLHVTIGVLMLLHHFGMSVA
                     GRIGQEDSLRIELVGLYWHFVDIVWVIIFTVVYLIPT"
     gene            4020..4370
                     /locus_tag="VF058_10935"
     CDS             4020..4370
                     /locus_tag="VF058_10935"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX6208855.1"
                     /translation="MTEDAHVAGRTEEDPRTHARVEEHAHPAPAEYVKVAVALAIVTA
                     LEVALFYIEGLADGLLIALLMVLMAIKFAMVAMWFMHLRFDNKIFSRLFITGILLAIG
                     VFTVVLVTIGVVGD"
     gene            4370..5209
                     /locus_tag="VF058_10940"
     CDS             4370..5209
                     /locus_tag="VF058_10940"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX6208856.1"
                     /translation="MPAWHPHPDAWLLVAVIVATYLYGLRRVGPRHVSPGDPPASRGQ
                     LVAFGLGVLALLVAADWPIHDLGERSLFTAHMLQHLLMTLVAPPLLLLGTPGWMLRWL
                     LRPRWLLSVIRALSKPLVALVLFNLVIALIHWPAFVTLYLREPLVHLATHAALVSTAL
                     LMWMPVLSPIVEIARLSYPLQMLYLFAQSIVPTVPASFLTFGSEPLYRIYEELPRLWG
                     ISALTDQRVAGLAMKLLGGLILWGVITILFFRWAKQEEEGVDDLGWRDVEEAVNPKEL
                     STR"
     gene            5206..5886
                     /locus_tag="VF058_10945"
     CDS             5206..5886
                     /locus_tag="VF058_10945"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX6208857.1"
                     /translation="MSEDQRRRSLLAIVVPLTAVVAIAIVLWSMSRILLAASETVAPV
                     IALLFALNILIAAALAATFRRRAGVVLAFVMAVLVPVLAVGIVGAVAGEREFHSAIEE
                     EPEPGPSPTEEPTEEPTEEPTEEPNGPSEGPTEATIVALNIAFDTDTMQLAANAEVTV
                     TLDNQDAGVPHNWAMYTDESASEEIFVGEIFPGPETMDYTFTTPGPGEYYFRCDVHPN
                     MQGTVTVG"
     gene            complement(5887..6870)
                     /gene="ccsB"
                     /locus_tag="VF058_10950"
     CDS             complement(5887..6870)
                     /gene="ccsB"
                     /locus_tag="VF058_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEX6208858.1"
                     /translation="MTEASNTTFGVALALYLVAMVGYLHHFAYRRPTPLTLARVVAYL
                     ALASHTVSVVLRGVAAGRVPWGNMYEYASVVGLAVVALYLVVAERRVEARRLGGFVLG
                     ATVLIMAASTMVYVEPGPLIPALNSGWLRIHVLAAILASALFTLGFVFTLLYLIQERR
                     ERRVERAVAERPAPAMASVGSGLPHDLEVESDPEEGRATVTLGPEQGNGHGGMPEGPT
                     RRGRLPGAAALDDLAAKAITFAFPIWTFAVIAGAIWAEQAWGRYWGWDPKEVWSFVTW
                     VLFAGYLHARATQGWRGRRAAVLAVLGFAALAFNLTVVNLVVSGLHSYAGV"
     gene            complement(6867..8345)
                     /locus_tag="VF058_10955"
     CDS             complement(6867..8345)
                     /locus_tag="VF058_10955"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEX6208859.1"
                     /translation="MATGTFSRGMLSVWRSLRSMRTALILLLLLGLGSVVGSLVPQAP
                     NSPARVAELFAERPLLARIYDALGFFDVYGSWWFTLLYVLLLISLAACLIPRTRGLIR
                     GLGSRWQPARDLEALRHYASGSVEGSPEEAVGRARRALRRRGWGVRSGGETALAAEKG
                     LAREVGSLAFHWAFFLLLLGVVYGKGFGFTGQATVIEGDVFTEAHASYDFPPREGRFF
                     GERHQGFQVRVLDFEVAYREGGLPRDFVSRVEVVEEGEVIRADEIRVNDPLEHLGVKL
                     YQSGYGWAPVVEVRHEGELLVSDPLVFVTDDPNDPVRPWRGAIKLPGISPQVGLELQL
                     LPDAAAFVAGMPMLEADDPFLAYRAYRGDLRLTEAQSVFRLEKELLEEYESGGLALGE
                     TVELPGGLEVTFRDVRQYTQLLVKRDPGIGLMLATALLVLVGLVPALYSSRRRIWVRA
                     VPEDGGTRLEIGGFALQRKAAFDEEFDRLVRDLVPRGRVRVP"
     gene            complement(8372..9160)
                     /locus_tag="VF058_10960"
     CDS             complement(8372..9160)
                     /locus_tag="VF058_10960"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX6208860.1"
                     /translation="MDLNVWWAPALAFAAGVVSFASPCVLPLVPGYVGFVLGQGADGR
                     PASPAGDGAAAGGAVAVAERPATRAALPMLLFVGGFALVFTLLGAFSRAFTEIFRHPW
                     GQRIAGGIVVLFGVYLILYSLRVGGPRLFAERRPFLSRVRPGTAGAFPLGMAFATGFT
                     PCIGPVLAGILTIAAAQGGGAWGALLMFTYSLGLGLPFVLVGLGLRRLMGALSWVKRN
                     YHWIAGVGGVLMLAIGVLLMLNLWTRFLAWSGLLDLVSGFEPPI"
     gene            9367..9693
                     /locus_tag="VF058_10965"
     CDS             9367..9693
                     /locus_tag="VF058_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208861.1"
                     /translation="MEPYKERADDFLRRWEWTWTSAFIAGLIVSFIAMTTLVVIPSWF
                     LFFANQTLGWSTADRLLVVIRDSIAMGWITVWTVAFIVVAYQVQVHRRKLRGERQAER
                     YSGGYR"
     gene            9712..>9915
                     /locus_tag="VF058_10970"
     CDS             9712..>9915
                     /locus_tag="VF058_10970"
                     /inference="COORDINATES: protein motif:HMM:NF024207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX6208862.1"
                     /translation="MAFQKTEVNIHEDYTITPVDPEWLKEGVKSPKHLMLDPDPCILC
                     RACEDVCPWNCIYMLDPGIVENPG"
BASE COUNT         1497 a         3599 c         3331 g         1488 t
ORIGIN      
        1 cgggtcgtcc gcgagcggac gacccgcgcc gcgatgcacc tcttccacta ctcgatcacc
       61 tacctcgggc tactcttcgc cgccgtcgcc gccgacacgc tgatcggagc cctggcgtga
      121 tcgaactctt cggcgacctg atcaggagcc acggctggca cgtcgtggcc gagctcctcc
      181 tgctggtcct ggccacggtg ttcaccgcct tctacgtcgc ggccctggtc cggggccggg
      241 tccgagccgt cgagtgcgcg ggctgcggca ggatcgcctc ccgggcccac gccgagtgcc
      301 cccgatgcgg gacctcgctg ccgacgtcgt gagcttcggc ccttccccga ccccgtgcta
      361 tcgtgtcgcc ggctcggggc ccgatcaggc cccgccacat ccgagatgag gtccgaccga
      421 gcgcgccgca cgccgagcac ccgcagacga tggtggctga cgggattcgt ggccaccacc
      481 gcgctgctcc tcgccgcctg ccagcagggg ctgccccagt cctcgctcga gccggccggg
      541 ccgcgagccg aacgcatcaa cgacctctcc acgatcgtct tctggatggc cggcgtcgtc
      601 ttcgtcctcg tggagggggc gatcatcttc atcacgatcc gctaccgccg ccgccgccgc
      661 gaggagcggg cgcccgtgca gatccacggc aacaaccgcc tggagatcct ctggacggtg
      721 atccccgcgc tcctgctggc cggcctggcc ttccccaccg tcgccgcgat cttcgacctc
      781 tcgcgccggc cgcccggagg cgacgtcctc gaggtcaccg tgatcgggca ccagtggtgg
      841 tgggagttcc gctaccccga cctcaacatc gtgacggcga acgagctcgt ggtccccgtg
      901 gacacgcccg tgcacctgag cctggagtcg gccgagaccc ccggcggcat cgccgagttc
      961 acggccgacg gccagcccgt cacccagaac gcgatccccg tgatccacag cttctgggtg
     1021 ccgaggctcg ccgggaagca ggacctcgtc ccgggcaagg tcgagacgct cacgatcgag
     1081 gccgacgaga ccggtacctt cctgggccag tgcaccgagt actgcggcac ctcccacgcc
     1141 aacatgcgca agcgggtgat cgtggagacc gagcagggct tccaggagtg ggtctcgcgg
     1201 atgcaagcgc cgccggcgga gccggagccg ggcagcctcg ccgcccaggg ggccgagttc
     1261 ttccagaact tcggcaccgg ctcctgcctg gcctgccacg gcgtggagcc cggagccggc
     1321 ggcacggtgg gccccaacct ggcccacctg gccagccgcg ggaccttcgg cgccggcctg
     1381 ttcgagacga accgggaaaa cctccagcgg tggctggacg acccccgggc gatgaagccg
     1441 ggcgtggtga tgcccgacta caacctgacc gaggagcaga tcgaggccat cgccgagtat
     1501 ctgctcacgc tcgaatgacg cgagaggtga acgactgatg gcgatcgcgc agcccctccc
     1561 ccggggctcg gtgttccggc gcccgaccgc cacgaccggg ttcgtgtcgt ggctcaccac
     1621 cgtggaccac aagcggatcg ggatccttta cggcgtcact gcgttcgcgt tcttcctgct
     1681 gggcgggctc gaggccctgc tgatccgctg gcagctcgcc acgcccgaag ggcagctgct
     1741 ggacgccgac gtctacaacc agctcttcac gatgcacggg atcacgatga tcttcctcgt
     1801 cgtgatgccc ctctcctccg cgttcttcaa ctacctcgtc ccgctgatga tcggggcccg
     1861 tgacgtggcc ttcccccggc tcaacgcgtt cagctactgg atcttcctgc tgggagggct
     1921 gttcctctac tcgtcctggt tcctgggcgg ggcccccgac ggagggtggt tcgggtacgc
     1981 ccccctctcc agccagatgc ccgggaacaa cctggacttc tacgcggtgg ggctccagat
     2041 cgtcggcgtg gcctcgctcg tctccgcggt gaacttcatc gtgacgatct tcaacgagcg
     2101 ggcgcccggg atgagcctga tgcggatgcc ggtcttcgtg tggatgacgc tcgtcgtctc
     2161 gttcctcctg ctgttcgccc tgccggtgat cgcgatcgcg ctgttccagc tggccgggga
     2221 ccgcatcctc gggacgcact tcttcgacgc ggcggcgggc ggggatccga tcctctggca
     2281 gcacatgttc tggatcttcg ggcaccccga ggtctacatc ctgatcctgc cggcgatggg
     2341 gatcgtctcg gagatcctcc ccaccttctc gcggaagccg ctgttcgggt acccggcggt
     2401 ggtgttcgcg ggcgccgcga tcgggttcat gggatggggc gtgtgggccc accacatgtt
     2461 cacctcgggg ctgggcccgg tggccaactc ggccttcgca ctctccacga tgttcatcgc
     2521 cgtccccacc ggcatcaaga tcttcaactg gctggggacg atgtggggag gggccatccg
     2581 gttcaccacg cccatgctga tgtcgatcgg gttcgtcgcc atgttcgtga tcggcgggct
     2641 gtccggggtc acgcactcga tcgtgcccgc ggactggcag cagcacgaca cctactacat
     2701 cgtggcgcac ttccactacg tgctgttcgg cggcgcgatc ttcgggctgt tcggaggggc
     2761 ctactactgg ttcccgaagt tcaccggccg gacctacagc gagccgctcg gccacctcca
     2821 cttctggctg atgctgatcg gcttcaacct gaccttcgcc ccgatgcacc tcctgggcct
     2881 cgaggggatg ccgcgccgga tctacaccta cggcgagggg ctgggcttcg acgtctggaa
     2941 catggtctcc acgatcgggg cgttcacgat cgcggcctcg gtgctcgtct tcatgtggaa
     3001 cgtggtccgg agcctctcgc gcggcgagaa ggcgccgccc gacccctggg acgggcgaac
     3061 cctggagtgg acgatccctt ccccgccgcc ccactacaac ttcgccgagg tcccccaggt
     3121 tcgggcgcgg gacgacttct ggcaccggaa gtacacagag gacgggtccg ggcgggtcca
     3181 gcccgtcgtg gccggcgcgg cgaccgagga ggccgaggag ggccacgcgg aggaggagca
     3241 cgcggaggag gagcacctcg acatccacat gcccgacccc tcgtactggc cgctcgtggc
     3301 cgcgtcgggg cttccgttca tcggctacgg cctgatctac gactggatct gggccgtgcc
     3361 gggcgcggtg ctcctgctgt tcggcctctt cggctggagc ctggagccgg tggcagagga
     3421 ggggcactga tggcgcaggc cgaggtccgc cacgaggccc acacggccac cgggctcgac
     3481 aaccggaagc tcgggatgtg ggccttcctc ggctcggaat gcctcttctt cggggcgctc
     3541 atcaccacgt acctcctcta ccgcggacgc agcgtccagg ggccgttccc cgaggacatc
     3601 tacgacatcc ccttcacctc ggtgagctcg ttcgtcctgc tgatgagctc cctgacgatg
     3661 gtgctggcgc tgtcggcgat ccagcgaggc gaccaccagg ggctccggat ctggctgctg
     3721 gccaccgcgt tcctgggcat gaccttcctg ggaggccagg tctacgagtt caccgtcttc
     3781 gtgaacgagg ggctgacggt cacgaggaac gtcttcggca gcgcgttctt cgtgctgacc
     3841 ggcttccacg ggctccacgt cacgatcggg gtgctgatgc tgctgcacca cttcgggatg
     3901 tcggtggccg gacggatcgg gcaggaggac agcctccgga tcgagctcgt gggcctctac
     3961 tggcacttcg tggacatcgt ctgggtgatc atcttcaccg tcgtctacct gatcccgaca
     4021 tgaccgagga cgcgcacgtc gcgggccgga ccgaggagga tccccgcacg cacgcgcggg
     4081 tggaggagca tgcccacccc gcgccggccg agtacgtgaa ggtcgcggtg gcgctggcga
     4141 tcgtcacggc cctcgaggtg gccctcttct acatcgaggg cctggccgac gggctcctga
     4201 tcgccctctt gatggtgctg atggcgatca agttcgccat ggtcgccatg tggttcatgc
     4261 acctgaggtt cgacaacaag atcttctctc ggctgttcat cacgggcatc ctcctggcga
     4321 tcggcgtctt caccgtggtg ctcgtgacga tcggggtggt gggcgactga tgccggcctg
     4381 gcacccgcat cccgacgcct ggctgctcgt cgccgtgatc gtcgccacgt acctgtacgg
     4441 cctccgccgc gtcggacccc gccacgtctc gccgggggat ccgccggcga gccgcggcca
     4501 gctggtcgcg ttcggactcg gtgtcctggc gctgctggtg gcggccgact ggccgatcca
     4561 cgacctcggc gagcggtccc tcttcaccgc gcacatgctc cagcacctgc tcatgacgct
     4621 cgtggcgccg cccctcctcc tcctggggac gcccggctgg atgctgcggt ggctgctccg
     4681 ccctcggtgg ctgctctcgg tgatccgcgc gctgtcgaag ccgctcgtgg cgctggtgct
     4741 cttcaacctc gtgatcgcgc tgatccactg gccggccttc gtgacgctgt acctgcggga
     4801 gccgctcgtc cacctcgcca cgcacgcggc gctcgtgagc accgccctgc tgatgtggat
     4861 gccggtcctc tcccccatcg tcgagatcgc gcgcctctcg taccccctgc agatgctcta
     4921 cctgttcgcg cagtcgatcg tgccgaccgt gccggcctcg ttcctgacgt tcggcagcga
     4981 gcccctgtac cggatctacg aggagctgcc gcgcctctgg gggatctcgg ccctcaccga
     5041 ccagcgggtg gcggggctgg ccatgaagct gctgggcggc ctcatcctct ggggcgtgat
     5101 cacgatcctc ttcttccgat gggcgaagca ggaggaggag ggcgtggacg acctcggctg
     5161 gcgcgacgtc gaggaggccg tcaacccgaa ggagctctcg acccgatgag cgaggaccag
     5221 cggcgccgct ccctgctcgc gatcgtcgtg cccctgacgg ccgtggtggc gatcgcgatc
     5281 gtgctctggt cgatgtcgcg gatcctcctg gcggcctcgg agactgtggc gcccgtgatc
     5341 gctctgctgt tcgcgctcaa catcctgatc gcagccgcgc tcgccgccac gttccgccgg
     5401 agggcgggcg tcgtcctggc gttcgtcatg gccgtgctcg tccccgtgct ggccgtgggg
     5461 atcgtgggcg cggtggccgg cgagcgcgag ttccactcgg cgatcgagga ggagcccgag
     5521 cccggcccga gccccaccga ggagccgacg gaggagccca ccgaggagcc caccgaggag
     5581 ccgaacggcc cgtccgaagg tccgaccgag gccacgatcg tggccctgaa catcgcgttc
     5641 gacaccgaca ccatgcagct cgcggcgaac gccgaggtga ccgtgacgct cgacaaccag
     5701 gacgcgggcg tcccccacaa ctgggcgatg tacacggacg agtcggcgag cgaggagatc
     5761 ttcgtgggcg agatcttccc cggacccgag acgatggact acacgttcac gaccccgggt
     5821 ccgggcgagt actacttccg ctgcgacgtc caccccaaca tgcaggggac cgtcaccgtc
     5881 gggtgatcac acgccggcgt acgagtgcag gcccgagacg acgaggttca ccacggtcag
     5941 attgaacgcc agcgcggcga accccaggac ggccagcacg gccgctcgcc tcccccgcca
     6001 gccctgggtc gcgcgcgcgt gcaggtagcc cgcaaagagc acccaggtga cgaacgacca
     6061 gacctccttc gggtcccagc cccagtagcg gccccaggcc tgttcggccc agatcgcccc
     6121 ggcgatgacg gcgaacgtcc agatcgggaa ggcgaacgtg atcgccttcg ccgcgaggtc
     6181 gtccagcgcg gccgctccgg gaagcctgcc ccgccgcgtc gggccctccg gcatcccgcc
     6241 gtgcccgttc ccctgctcgg gaccgagcgt cacggtggcc cgcccctcct cggggtcgga
     6301 ctcgacctcg aggtcatgcg gcaggcccga gcccaccgac gccatggccg gcgccgggcg
     6361 ctcggccacg gcccgctcca cccgccgctc ccgccgctcc tggatcaggt acagcaaggt
     6421 gaagacgaag ccgagcgtga agagcgccga ggccaggatc gccgccagca cgtggatccg
     6481 cagccacccc gagttgagcg ccgggatcag cgggccgggc tcaacgtaga ccatcgtgga
     6541 ggcggccatg atcagcaccg tggccccgag gacgaagccg ccgagccgcc gagcctccac
     6601 tcgccgctcg gcgacgacga ggtacagagc cacgaccgcc aggcccacga cgctcgcgta
     6661 ctcgtacatg ttgccccagg ggacccgtcc cgcggccaca ccccggagga cgacggagac
     6721 cgtgtgcgag gcgagcgcca ggtacgcgac gacccgggcc agggtcagcg gcgttggtcg
     6781 ccggtacgcg aagtggtgga ggtacccgac catcgccacc aggtagaggg ccagcgcgac
     6841 cccgaacgtc gtgtttgagg cctcggtcat gggaccctca ctctaccgcg gggcaccagg
     6901 tcgcgaacga gacgatcgaa ctcctcgtcg aaggccgcct tccgctggag ggcgaagccc
     6961 ccgatctcga gccgggtgcc gccgtcctcg ggcacggccc gcacccagat ccggcgacgg
     7021 ctggagtaga gggccgggac gaggccgacg aggacgagca gggcggtggc cagcatcagc
     7081 ccgatccccg ggtcgcgctt cacgaggagc tgggtgtact ggcgaacatc gcggaacgtg
     7141 acctccaggc cgcccgggag ctcgaccgtc tctcccagcg ccaggccgcc gctctcgtac
     7201 tcctcgagca gctccttctc caggcggaag accgactgcg cctcggtcag ccggaggtcg
     7261 cctcggtacg cgcggtaggc gaggaagggg tcgtccgcct cgagcatcgg catgcccgcc
     7321 acgaacgccg cggcgtcggg cagcagctgc agctccaggc ccacctgcgg tgagatcccc
     7381 gggagcttga tcgccccccg ccaggggcgg accgggtcgt tcgggtcgtc ggtgacgaac
     7441 acgagcgggt cggagacgag cagctcgccc tcgtggcgca cctcgaccac cggcgcccac
     7501 ccgtagcccg actggtagag cttcaccccg aggtgctcca gcgggtcgtt gacgcggatc
     7561 tcgtcggcgc ggatcacctc gccctcctcc accacctcca cccgcgagac gaagtcgcgc
     7621 ggcagccccc cttcgcggta cgccacctcg aagtcgagca cgcggacctg gaacccctgg
     7681 tgccgctcgc cgaagaaccg cccctcccgg ggcgggaagt cgtagctggc gtgggcctcg
     7741 gtgaagacgt cgccctcgat caccgtggcc tggcccgtga acccgaaccc cttcccgtag
     7801 accaccccca ggagcaggag gaagaacgcc cagtggaagg ccaggctgcc cacctcccgg
     7861 gccaggccct tctcggcggc cagcgccgtc tcgcccccgc tccgcacgcc ccagccccgg
     7921 cgccggagcg cccggcgggc acggcctacg gcctcctcgg gcgacccctc gacgctgccg
     7981 ctcgcgtagt gccggagcgc ctcgaggtcg cgagccggct gccaccgcga gcccagcccc
     8041 cggatcaggc cgcgcgtccg cgggatcagg cacgcggcca gcgagatcag gaggaggacg
     8101 tagagcagcg tgaaccacca cgacccgtac acgtcgaaga accccagtgc gtcgtagatc
     8161 cgcgccagca gcggccgctc ggcgaagagc tcggccacgc gggccggcga gttgggcgcc
     8221 tggggcacga gcgaccccac cacgctcccc agccccagca ggaggagcag gatcagcgcc
     8281 gtccgcatcg agcggagcga gcgccacacc gagagcatgc cgcgcgagaa ggtcccggtc
     8341 gccatcgccc ccgatcgtac gcgggcgccg actagatcgg gggctcgaag ccgctgacga
     8401 ggtcgagcag gcccgaccag gccaggaacc gcgtccagag gttgagcatc agcaggacgc
     8461 cgatggccag catcaggacg ccgcccaccc ccgcgatcca gtggtagttg cgcttgaccc
     8521 aggagagcgc gcccatcagc cggcgaagcc ccaaccccac gagcacgaac ggcaggccca
     8581 ggccgaggga gtacgtgaac atcagcagcg caccccaggc tcccccgccc tgggcggccg
     8641 cgatcgtgag gatccccgcc agcaccgggc cgatgcacgg cgtgaacccg gtggcgaacg
     8701 ccatccccag cgggaacgcc cccgcggtgc ccgggcgcac cctcgacagg aacgggcgcc
     8761 gctccgcgaa gaggcgaggc cccccgaccc tgagcgagta gaggatcagg tagaccccga
     8821 acaggacgac gatgcccccg gcgatccgct gtccccacgg gtggcggaag atctccgtga
     8881 aggcccgcga gaaggcgccg aggagcgtga agacgagcgc gaaccccccg acgaagagca
     8941 gcatcgggag ggcggcccgc gtcgccggcc gctcggccac cgccaccgcc ccgcccgccg
     9001 ccgcgccgtc cccggccggt gacgcgggcc gcccgtccgc gccctgaccg agcacgaagc
     9061 cgacgtaccc cggcaccagg gggagcacgc agggcgaggc gaacgacacc acgccggccg
     9121 cgaaggccaa cgccggcgcc caccagacgt tcagatccac gcttcctcgc cccctcggtt
     9181 gcgaacggct cccagtatgc gcatgattcc ggatgacgga cttgtgaaag gattcacaag
     9241 ccgtgcgagc gggaggctcg cgcggccggg gtttggggag gcggagggcc gcacgataga
     9301 atcggcccgc cccgaggagt ggagagcgga gtgagactgc cgatcccgag cagcgtcagg
     9361 cgcaggatgg agccctacaa ggagcgcgcc gacgacttcc tcaggcgatg ggagtggacg
     9421 tggacgtccg ccttcatcgc cgggctgatc gtctcgttca tcgccatgac gacgctggtg
     9481 gtgatcccct cctggttcct cttcttcgcc aaccagaccc tggggtggtc gaccgccgac
     9541 cgcctgctcg tcgtgatccg cgactcgatc gcgatgggct ggatcaccgt gtggacggtg
     9601 gccttcatcg tcgtcgccta ccaggtccag gtgcaccgcc gaaagctccg tggcgagcgt
     9661 caggccgaac ggtactctgg gggctaccga tagtgcgagg ggtgagctga cgtggccttc
     9721 cagaagaccg aggtgaacat ccacgaggac tacacgatca cgcccgtgga cccggagtgg
     9781 ctgaaggaag gggtgaagtc cccgaagcac ctgatgctcg acccggatcc ctgcatcctg
     9841 tgccgggcct gcgaggacgt ctgtccctgg aactgcatct acatgctcga cccggggatc
     9901 gtggagaacc ccggc
//
LOCUS       DASUTW010000317         2116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_107608, whole genome shotgun sequence.
ACCESSION   DASUTW010000317 DASUTW010000000
VERSION     DASUTW010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107608"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2116)
                     /locus_tag="VF058_10975"
     CDS             complement(<1..>2116)
                     /locus_tag="VF058_10975"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6208863.1"
                     /translation="PEEARFCQACGSPMVQAPAEWEQRKTVTVVFADVTGSTALGEAL
                     DPESLRRLMARFFMEMSAVLQRHGGSVEKFIGDAVLAVFGRPTVHEDDALRAVRAAAE
                     MRQVLRSLNEELQRAHGVTLQIRTGVNTGTVIIGDLVAGQSFVAGDAINVAARLEQLA
                     SPGEILIGEDTYRLVRHAVTAEPAPPLKVRNREEPVVAYRLEDVHDAAPRGESPLRSP
                     MVGRVRDLGVLLDLVDEVTKRGDAHLATILGPAGVGKSRLVEELVAGLGEQLPSLSGR
                     CLTYGEGITYWPVSEVVRQALGIRGESSPAEIRRLLAERVRGSDPGDRIVEGVAQMMG
                     LSDAAGGQEQMFWGTRRFLEVVAGEKPLLVILDDVHWGEPTFLDFVEYLADFASAPLL
                     LVCMARLDLLEERPGWGTGRRNATTLTLDPLTEGESVALIRNLLGIEDLEPAAGRHLI
                     RATEGNPLFIEEMLRMLIDEGLVKQDDEGWASVGDVTSLPAPPTIQALLSARLARLRP
                     EELAAIERASVVGKVFSWSSVAALTPEEERSEVGGHLQALVRKELILPDTEVIEGEDT
                     FRFRHLMIRDAAYEAIPKEVRADLHELLASWLEARGGEEYDEILGHHLEQAHRWRTEI
                     APADNRSRALAERAVEQFASAGRRAFARGDVPATVRLLSHAVALVPDEHPTRPRLLAD
                     LGRALMEHGELERADEVLTEAAR"
BASE COUNT          287 a          790 c          737 g          302 t
ORIGIN      
        1 accgcgcggc ctccgtcagc acctcgtccg cccgctccag ctccccgtgc tccatcaggg
       61 cccttcccag gtctgccagc aggcgcggcc gcgtcgggtg ctcgtccggg accagggcca
      121 cggcgtgtga gaggaggcgg acggtggccg ggacgtcccc ccgggcgaac gccctccgac
      181 ccgccgacgc gaactgctcc acggctcgct cggcgagcgc ccgcgatcgg ttgtcggccg
      241 gcgcgatctc ggtcctccat cggtgggcct gctccaggtg gtggccgagg atctcgtcgt
      301 actcctcgcc tcctcgcgcc tccagccagg aggccagcag ctcgtggagg tcggcccgga
      361 cctccttcgg gatcgcctca taggccgcgt cccggatcat gaggtgtcgg aaccggaagg
      421 tgtcctcgcc ctctatcacc tcggtgtccg ggaggatcag ctccttgcgc acgagcgcct
      481 ggagatgacc cccgacctcc gatcgttcct cctccggggt gagcgccgcc accgacgacc
      541 acgagaagac cttccccacg acgctggccc gctcgatcgc cgcgagctcc tcgggccgga
      601 gacgcgccag ccgcgccgag agcagggcct ggatcgtggg cggggccggg aggctggtca
      661 cgtcccccac gctcgcccac ccctcgtcgt cctgcttcac gagaccctcg tcgatcagca
      721 tccggagcat ctcctcgatg aacagggggt tgccctcggt ggcccggatc aggtggcggc
      781 ccgcggcggg ctcgaggtcc tcgatgccga ggaggttccg gatcagcgcg acgctctcgc
      841 cctcggtcag ggggtcgagg gtcagggtcg tggcgttgcg ccgtccagtg ccccagcccg
      901 gcctctcctc caggaggtcg agccgggcca tgcagacgag gagcagcggg gcggaggcga
      961 agtccgccag gtattccacg aagtcgagga acgtgggctc gccccagtgg acgtcgtcga
     1021 ggatcacaag gaggggcttc tcgcccgcca cgacctcgag gaaccggcgc gtgccccaga
     1081 acatctgctc ctggccgccg gctgcgtcgc tgaggcccat catctgggcc acgccctcca
     1141 cgatccggtc gccggggtcg ctgcctcgga cccgctcggc gaggagcctc cggatctcgg
     1201 cgggcgacga ctcccctcgg atcccgaggg cctgccgcac gacctcgctg acgggccagt
     1261 acgtgatccc ctcgccgtag gtgaggcagc gtcccgagag cgagggcagc tgctccccga
     1321 gcccggcgac gagctcctcc accagacgcg acttccccac gcccgccggc cccaggatcg
     1381 tggccaggtg cgcgtccccc cgcttcgtca cctcgtccac gagatcgagg aggacgccca
     1441 ggtccctgac gcgtccgacc atgggggagc ggaggggcga ctcgccgcga ggggcagcgt
     1501 cgtgcacgtc ctcgaggcgg taggcgacca ccggctcctc gcggttgcga accttcaggg
     1561 gcggggcggg ctcggcggtg acggcgtgtc ggacgagccg gtaggtgtcc tcgccgatca
     1621 ggatctcgcc gggggaggcc agctgctcga gccgggccgc gacgttgatc gcgtcaccgg
     1681 cgacgaagga ctgcccggcc acgaggtccc cgatgatgac cgtgcccgtg ttgacgccgg
     1741 tcctaatctg cagggtcaca ccgtgggcac gctgcagctc ctcgttgagc gagcggagca
     1801 cctgccgcat ctcggccgcg gcccggacgg cgcggagggc gtcgtcctcg tgcacggtgg
     1861 gccgcccgaa caccgccagc accgcgtcgc cgatgaactt ctccacgctg cctccgtggc
     1921 gctggaggac ggcgctcatc tccatgaaga agcgcgccat cagccgccgc agcgactctg
     1981 ggtcgagggc ctctcccagc gcggtggacc ccgtgacgtc ggcgaacacg accgtgacgg
     2041 tcttccgctg ctcccactcc gcgggggcct ggaccatggg cgacccgcag gcctgacaga
     2101 agcgggcctc ctcggg
//
LOCUS       DASUTW010000318         4843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_885631, whole genome shotgun sequence.
ACCESSION   DASUTW010000318 DASUTW010000000
VERSION     DASUTW010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885631"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VF058_10980"
     CDS             complement(<1..660)
                     /locus_tag="VF058_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6208864.1"
                     /translation="MTLERRTAEVSAGELAYVEAGEGKPILFLHGIPTSADLWRNVAP
                     LLAPWARVIVPDLLGYGASAKPEGAELHIRAQARCARELLEALGVERFAVVGHDLGGG
                     IAQLLAIEGGVEALVLTNSISWDGWPVEGVRRVQGLPEEGWDEALAEKVVRRSFERGM
                     ARFERLSQEDLEGYVAPWRGDPLALRRAFLSVDGVGLEGTEEALGALGVPAFVLWGED
                     DP"
     gene            complement(657..1490)
                     /gene="folP"
                     /locus_tag="VF058_10985"
     CDS             complement(657..1490)
                     /gene="folP"
                     /locus_tag="VF058_10985"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011057362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX6208865.1"
                     /translation="MTTWKLPDRAYDCEERTLVMGVLNVTPDSFSDGGRFFDPEDAVK
                     QALRMVEDGADIVDVGGESTRPGSDPVPPDEERRRVVPIVERLAQELEVPISIDTRKA
                     EVAAAAVRSGATIVNDVTAGGDPEMFDVVRESGTALVLMHMKGEPKTMQEDPHYDDVV
                     WEVRDYLAERVEAAVAAGIDREAICVDPGLGFAKTTPHSLAMMKHTDALLDLGRPVLV
                     GPSRKSFIGDVLGTPVTDRAEGTVGAVCVMVAKGAHIARVHDVLETVRAVRVVDAILR
                     A"
     gene            complement(1592..2365)
                     /locus_tag="VF058_10990"
     CDS             complement(1592..2365)
                     /locus_tag="VF058_10990"
                     /inference="COORDINATES: protein motif:HMM:NF038123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spondin domain-containing protein"
                     /protein_id="HEX6208866.1"
                     /translation="MTVIAPEGGIHMRIRALITNLAVVALLSAFAGPHAAAAAPERTF
                     RVTVTNLTEGQPLTPPVVATHRGAYDAFDVGAPASEGLQQIAENGNGAPLLAELDASK
                     HVDASLQTGTGPLVPDGLPGSAMFADSVTFEIIAGPGASFLTYVSMLICTNDGFTGAD
                     GIKLPNRVGETATAATFGYDAGTEINTEDFADIVPPCQALVGVTSDDAGTGMTNPALD
                     EGGAIHHHDGIAGIADLVPAVHGWDTVAPVATISITRVA"
     gene            2458..3135
                     /locus_tag="VF058_10995"
     CDS             2458..3135
                     /locus_tag="VF058_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012886932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6208867.1"
                     /translation="MSERVLVVDDEPIVREVLGRYLEREGFEVGTAADGEEAIERFAD
                     APPDLVVLDLMMPKLDGAGVLSWIRRRAPTPVIMLTARGEETDRVVGLEMGADDYVTK
                     PFSPREVVARVRAVLRRSGSDPGNGTIELGDLEVDPWSRLAALRGRRLTLTPKEFDLL
                     HHFASNPRVVFSRTELLEELWDFAFDGDPSTVTVHVRRLREKIEDDPSHPRHLVTVWG
                     VGYRFDP"
     gene            3132..4334
                     /locus_tag="VF058_11000"
     CDS             3132..4334
                     /locus_tag="VF058_11000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6208868.1"
                     /translation="MSARALLGLGLLGAAGAGATVAIAAATGMPRADLAELSFLLAVA
                     VLATVGVTATAARLLARSSFRVRIAAASLAGVAVALLNLGAFALLMFVSGHDAALTAV
                     LLAYTGTVGVAAGLALGRSSRDGVARLEETARHLAEGDLDARVGRVEAGPELDTLART
                     LDEMASRLQEAQRREREAESRRRDLVAAVSHDLRTPLAGLRAMVEAVEDGVVDDPEDL
                     RRYAHEMLRAVAALSTLVDDLFELSQLDADAIREEARRASLADVVASALAAVEPQRLA
                     KGVSVETDLADAATEPCSPRVTRVLQNLIQNAIRHTPADGSVRVEARRDAGRLEIAVT
                     DTGEGIPVGSLDRVFEPFWRGDAARSGPGSGLGLALAKRLVTAMGGDISVESRPREGS
                     RFAVLLPS"
     gene            4394..>4843
                     /locus_tag="VF058_11005"
     CDS             4394..>4843
                     /locus_tag="VF058_11005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEX6208869.1"
                     /translation="MMQTRALSVTETGRLGELYERHAPDAVRLGYLMTGDAALAEDLV
                     HEAFVKLLGRFRDLRNPDSFHWYLRKTVVNLARSHFRHARVEREYVEREGRRPVDRPD
                     ADPTERDEMWAALGRLSERQRAAIVLRYYEDLTEAQTAEVMGVRLGTV"
BASE COUNT          652 a         1779 c         1732 g          680 t
ORIGIN      
        1 cgggtcgtcc tcgccccaga gcacgaaggc cgggacgccg agcgcgccga gcgcctcctc
       61 ggtcccctcc agccccacgc cgtccaccga caggaaggct ctccgcagcg cgagcgggtc
      121 cccccgccag ggagcgacgt atccctcgag gtcctcctgc gagagccgct cgaacctggc
      181 catcccgcgc tcgaacgacc gccgcacgac cttctcggcc agcgcctcat cccaaccctc
      241 ctcgggcagc ccctgcaccc tccgaacgcc ctccaccggc cacccgtccc aggagatcga
      301 gttcgtcagg acgagcgcct cgaccccgcc ctcgatcgcc aggagctggg cgatccctcc
      361 ccccaggtcg tggcccacga ccgcgaaccg ctcgaccccg agcgcctcga ggagctcgcg
      421 cgcgcacctg gcctgcgccc ggatgtgcag ctccgccccc tcgggcttgg ccgatgcccc
      481 gtatcccagg aggtcgggga cgatcaccct ggcccagggc gcgaggagcg gcgccacgtt
      541 gcgccacaga tcggcgctcg tggggatccc gtggaggaag aggatcggct tcccttcccc
      601 ggcctcaacg tacgcgagct cccccgccga gacctccgcg gtccggcgct ccagggtcat
      661 gcccgaagga tcgcgtctac gacccgcacc gcgcggacgg tctccaggac gtcgtggacc
      721 cgggcgatgt gggcgccctt ggccaccatg acgcagacgg cgcccacggt gccctccgcg
      781 cggtcggtca ccggggtccc caggacgtcg ccgatgaacg acttccggga tggccccacc
      841 agcacgggcc gtcccaggtc cagcagggcg tcggtgtgct tcatcatcgc cagggagtgc
      901 ggggtcgtct tggcgaagcc caggcccggg tccacgcaga tcgcctcgcg gtcgatcccc
      961 gcggccaccg ccgcctccac gcgctcggcc aggtagtccc ggacctccca aaccacgtcg
     1021 tcgtagtggg gatcctcctg catcgtcttc ggctcgccct tcatgtgcat caggacgagg
     1081 gcggtgccgc tctcccggac cacgtcgaac atctcgggat cgccgcccgc ggtgacgtcg
     1141 ttgacgatcg tggcaccgga ccggacggcg gccgcggcca cctcggcctt cctcgtgtcg
     1201 atcgagatcg gaacctccag ctcctgcgcc agccgctcca cgatgggaac gacgcgacgc
     1261 cgctcctcat cgggcggcac ggggtcggaa ccgggtcggg tggactcccc gcccacgtcc
     1321 acgatgtcgg cgccgtcctc gaccatccgg agggcttgct tcaccgcgtc ctcggggtcg
     1381 aagaaccggc caccgtccga gaacgagtcg ggggtgacgt tcagcacgcc catcaccagc
     1441 gtgcgctctt cgcagtcgta ggcccggtcg ggcagcttcc aggtggtcac ggccacagcg
     1501 tacccgcggc ggcgggcggc cgcccacccc ggccgtcccc tcccgggacg agccgcggcc
     1561 cgccgcccgg gagggggacg actcgaagat gtcaggcgac ccgcgtgatc gagatcgtcg
     1621 cgaccggcgc gaccgtgtcc cagccgtgga ccgcggggac gaggtccgcg atgcccgcga
     1681 tcccgtcgtg atggtggatg gccccaccct cgtcgagcgc ggggttcgtc atgccggttc
     1741 ccgcgtcatc gctcgtcacg cccacgagcg cctgacacgg cgggacgatg tccgcgaagt
     1801 cctccgtgtt gatctcggtg cccgcgtcgt acccgaacgt ggccgcggtg gccgtctcgc
     1861 ccacacgatt gggcagcttg atcccgtcgg cgccggtgaa gccgtcgttc gtgcagatca
     1921 gcatcgacac gtacgtgagg aagctggctc ccgggcccgc gatgatctcg aacgtcaccg
     1981 agtctgcgaa catcgccgac cccggcagcc cgtccggcac gagcggtccg gtccccgtct
     2041 gcagggacgc gtcgacgtgc ttggaggcgt cgagctccgc cagcaacggc gccccgttcc
     2101 cgttctcggc gatctgctgg agcccctcgc tggccggtgc gcccacgtcg aacgcgtcgt
     2161 aggctccccg gtgcgtggcc accaccggcg gcgtgagggg ctgcccctcg gtcaggttcg
     2221 tgaccgtgac ccggaacgtc cgctcgggcg cggccgccgc agcatgaggc ccggcgaacg
     2281 cgctcagcag cgcgaccacc gccaggttcg tgatgagggc gcggatgcgc atgtggatcc
     2341 ctccttccgg ggcgatgacc gtcaccgccc acgatccaag gtacgaacgg tccccttacc
     2401 gaacccttgc ggcgatccgt aagctcctcg taaggaaggt gcgagaggat gggagacatg
     2461 agcgagcgtg tcttggtcgt ggacgacgag cccatcgtgc gcgaggtgct ggggaggtac
     2521 ctggagcgcg agggcttcga ggtgggcacg gccgccgacg gcgaggaggc gatcgagcgc
     2581 ttcgccgacg ccccgcccga tctggtcgtc ctcgacctca tgatgccgaa gctcgacggg
     2641 gccggggtcc tgtcgtggat ccgccgacgg gccccgaccc cggtgatcat gctgaccgcc
     2701 cggggcgagg agaccgaccg ggtggtgggg ctggagatgg gcgccgacga ctacgtgacg
     2761 aagccgttct ccccgcgcga ggtggtggcc cgcgtccgtg ccgtccttcg tcgctccggg
     2821 agcgaccccg ggaacggcac gatcgagctc ggcgacctcg aggtcgaccc gtggtcccgc
     2881 ctggcagccc tccgagggcg gcgcctcacc ctcaccccca aggagttcga cctcctccac
     2941 cacttcgcct ccaacccgcg cgtcgtgttc tccaggacgg agctgctcga ggagctgtgg
     3001 gacttcgcct tcgacggcga cccctccacg gtgacggtcc acgtgcgccg ccttcgggag
     3061 aagatcgagg acgatccctc gcacccccga cacctcgtga cggtgtgggg ggtcggctac
     3121 cggttcgacc catgagcgcg agggcgctgc tcgggctggg gctgctgggg gccgcgggcg
     3181 ccggggccac cgtggcgatc gccgcggcca ccgggatgcc gcgggccgac ctcgccgagc
     3241 tctcgttcct cctggctgtc gcggtcctgg ccaccgtggg cgtcacggcc accgcagccc
     3301 ggctcctggc ccgctcgtcg ttccgggtcc gcatcgcggc ggcgtcgctg gcgggcgtgg
     3361 ccgtggcgct gctcaatctc ggggcgttcg cgctcctgat gttcgtgagc ggacacgacg
     3421 ccgcgctcac ggccgtcctg ctcgcctaca cggggacggt gggcgtcgcg gccggtctgg
     3481 ccctgggaag gagctcccgg gacggcgtgg cccggctcga ggagacggcc cgacacttgg
     3541 cggaggggga tctggacgcg cgggtgggtc gcgtggaggc cggtcccgag ctggacaccc
     3601 tggcccgcac cctcgacgag atggcctccc ggctccagga ggcccagcgg agggagcggg
     3661 aggccgagtc gaggcgccgg gacctcgtgg ccgccgtctc gcacgacctg cgaacgccgc
     3721 tggccgggct ccgggcgatg gtggaggccg tcgaggacgg cgtggtggac gaccccgagg
     3781 acctccggcg gtacgcgcac gagatgctga gggcggtggc tgcgctgagc accctcgtgg
     3841 acgacctctt cgagctgtcc cagctcgacg ccgacgcgat cagggaggag gcgcggaggg
     3901 cgagcctggc cgacgtggtc gcgtcggccc tcgccgccgt ggagccccag cgcctggcca
     3961 agggagtctc ggtggagacg gacctggccg acgcggcgac cgagccgtgc tcgccccggg
     4021 tcacccgcgt cctgcagaac ctcatccaga acgcgatccg tcacacgccg gcagacggca
     4081 gcgtccgggt cgaggcccgg cgcgacgccg gccggctcga gatcgcggtc accgacaccg
     4141 gggagggcat cccggtgggc tcgctggacc gggtgttcga gccgttctgg cggggcgatg
     4201 ccgcccgttc cggccccggg tccggcctgg ggctggccct ggccaagcgg ctggtgacgg
     4261 cgatgggcgg cgacatcagc gtggagagcc gcccccgcga ggggtcgcgc ttcgccgtgc
     4321 tcctcccctc ctgagccctc gtgacacgga accttccaag gccctgaggc gtcttgtgct
     4381 cggaggatgg gccatgatgc agacgagggc gttgagcgtg accgagacgg ggaggctggg
     4441 cgagctgtac gagcggcacg cccccgacgc cgtccggctc gggtacctga tgaccggcga
     4501 tgccgcgctg gccgaggatc tcgtccacga ggcgttcgtc aagctgctgg gccggttccg
     4561 cgatctgcgg aacccggact cgttccactg gtacctccgg aagaccgtgg tgaacctcgc
     4621 ccggtcgcac ttccgccacg cgagggtgga gcgggagtac gtggagaggg agggccggcg
     4681 ccccgtcgat cgaccggacg ccgacccgac ggagcgcgac gagatgtggg cggcgctcgg
     4741 aaggttgtcc gagcgccagc gcgccgcgat cgtcctccgc tactacgagg acctgaccga
     4801 ggcccagacg gccgaggtga tgggggtccg tctggggacg gtg
//
LOCUS       DASUTW010000319         4416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1790824, whole genome shotgun sequence.
ACCESSION   DASUTW010000319 DASUTW010000000
VERSION     DASUTW010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1790824"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VF058_11010"
     CDS             complement(<1..972)
                     /locus_tag="VF058_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016467678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX6208870.1"
                     /translation="MLLIELGVVILGLAVLARVAERTGFSPIPLYLLVGLAFGDGGLF
                     PLVTSEEFIEVGAEIGLILLLLMLGLEYAGDELTESLRRNAGAGLVDLAFNFLPGFAA
                     GLLLGWGLVPALFLGGITYTSSSGIVAHMIQQLGWVANAETPTILSILVIEDLAMAGY
                     LPLMEVLTRGDEIASATIAIVAAVSAVLVTLFVAVRFGPSLSRLAFSRSDEGLLLTIV
                     GIALVVAGLAERLQVSAAVGAFLVGIALSGHAADRARVLLTPLRDLFAAVFFIFFSLQ
                     IDPSAIPSVLGVAAALALVTGASKVATGWVSAGRRGLGSGARLRAGTA"
     gene            complement(989..1471)
                     /locus_tag="VF058_11015"
     CDS             complement(989..1471)
                     /locus_tag="VF058_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017869955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter regulatory subunit"
                     /protein_id="HEX6208871.1"
                     /translation="MAEVEETKLPGLGIRYEFLTSRGSRIGVVHHRTGRRELFVHDQD
                     DDTSGTIAALDEDDSRTLAELLGGSRVGEQLANLQQRVEGIAIDWLPIAEGTPFAGKS
                     IGDTRARTLTGTSIVAIVRGEDAVPAPGPEHPIEAGDTLVVVGTPRGIEQLAVILRTG
                     "
     gene            complement(1488..2237)
                     /locus_tag="VF058_11020"
     CDS             complement(1488..2237)
                     /locus_tag="VF058_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEX6208872.1"
                     /translation="MSDRGPRREAAASGAKRDAASRREPKAPAEPSISLEEVQEHPVL
                     SAFIAQADKQMDGMGFTEHGFRHAKLTGKIAYNVLDRLGHDPRTTTLGSVAGYLHDIG
                     NCISRTDHGQTGALLVNHLLGSKVEPADLAVVMGAIGNHEEEYGHAVGPVAAAVILAD
                     KSDVHRSRVRKADQIEFDIHDRVNYAVEESFLRVDADAGTITLELTIDTSISHVMEYF
                     EIFLDRMAMCRRAAEELECRFRVVINETEVL"
     gene            complement(2234..2563)
                     /gene="yajC"
                     /locus_tag="VF058_11025"
     CDS             complement(2234..2563)
                     /gene="yajC"
                     /locus_tag="VF058_11025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX6208873.1"
                     /translation="MTALPIAQAGGQGNVTFLISLVLLVAIFYFLLIRPQQRRMRQQR
                     ELVESLAVGDEVITIGGLYGRIMEMDDESVTVEISPGTRVRFVKSAIARRLTPEPGEP
                     DEGVIEE"
     gene            complement(2649..3668)
                     /gene="ruvB"
                     /locus_tag="VF058_11030"
     CDS             complement(2649..3668)
                     /gene="ruvB"
                     /locus_tag="VF058_11030"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX6208874.1"
                     /translation="MTEEPFADERLVDADALDPEFDAALRPRSLDEFVGQERIKEQLG
                     LLIAGARERREPVDHVLFSGPPGLGKTTLAGILANEMGVALRQTSGPALERTADLAAI
                     LTNLEEHEVLFVDEIHRMPRAVEEVMYPALEEFKLDVVIGKGPSARTIRLDLPRFTLV
                     AATTRPGLITLPLRERFGFSPRLEYYGEEDLAKVVARSAGILQVAVDPEGAEEIATRS
                     RGTPRIANRLLKRVRDYAQVRADGRIDREIACDALALFEVDELGLDRVDTLILTAMIE
                     RFGGGPVGLSTLSAAVGEDAGTLEDVYEPYLLQSGFIQRTPRGRIVTEMARRHLGMPA
                     SGRLV"
     gene            complement(3665..4255)
                     /gene="ruvA"
                     /locus_tag="VF058_11035"
     CDS             complement(3665..4255)
                     /gene="ruvA"
                     /locus_tag="VF058_11035"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX6208875.1"
                     /translation="MIAFVEGVLAERGADRVVVNAGGLGYELLVSTTTLAALPAVGKP
                     SRLLTHLQVRDDSMVLFGFSSGAERDLFELLITVSGVGPKMAMAVLSTLEPDTLRRAV
                     LDGDIDAVTVVPGVGKKVAGRIVLDLRDKLGGEIDLPGAGPLAEVRQALEGMGLSPQE
                     IQGAVGELDVDGRPVEELLREALRRVGGREAAGAKR"
BASE COUNT          655 a         1645 c         1506 g          610 t
ORIGIN      
        1 ggccgtcccg gcccgcaggc gggcccccga ccccagcccc cgcctgccgg cgctcaccca
       61 gccggtggcc accttggagg cccccgtgac gagcgccagg gcggcggcca cgccgagtac
      121 cgatgggatc gccgacgggt cgatctggag gctgaagaag atgaagaaga cggccgcgaa
      181 caggtcgcgg agcggcgtca gcagcacccg cgcccggtcg gccgcgtggc cggagagcgc
      241 gatccccacg aggaacgccc ccacggcagc ggagacctgg agccgctcgg ccagcccggc
      301 caccaccaga gcgatcccca cgatcgtcag gagcaggccc tcgtccgagc ggctgaacgc
      361 gagcctgctg agggaggggc cgaaccgcac ggccacgaac agcgtgacca ggaccgcgct
      421 cacggcggcg acgatggcga tggtcgccga cgcgatctcg tccccgcggg tcagcacctc
      481 catcagcggc aggtatcccg ccatggccag gtcctcgatg acgaggatcg agaggatcgt
      541 cggggtctcc gcgttcgcca cccaccccag ctgctggatc atatgggcca cgatccccga
      601 cgaggaggtg taggtgatcc cgcccaggaa gagcgccggc acgaggcccc atcccagcag
      661 cagccccgcg gcgaagcccg ggaggaagtt gaaggccagg tccacgaggc cggccccggc
      721 gttcctgcgc aggctttccg tcagctcatc gcccgcgtac tcgagaccca gcatgaggag
      781 caggaggatc agcccgatct cggcgcccac ctcgatgaac tcctcggacg tcaccaaggg
      841 gaagagcccg ccgtcgccga acgccaggcc gacgaggagg tagaggggga tcggcgagaa
      901 ccccgtccgc tcggcgaccc gggccaggac ggcgagcccc aggatcacca cccccagctc
      961 gatcaggagc acgggggtgg acactggctc agccggtccg aaggatcacg gcgagctgtt
     1021 cgatccctcg gggcgtcccc acgacaacca gggtatcgcc cgcctcgatc gggtgctcgg
     1081 ggcccggagc ggggacggcg tcctcgccgc ggacgatcgc cacgatggag gtgcccgtca
     1141 gggtcctggc cctcgtgtcg ccgatcgact tccccgcgaa gggcgtcccc tcggcgatgg
     1201 gcagccagtc gatcgcgatc ccctccaccc gctgctgcag attcgccagc tgctccccta
     1261 cccgcgaccc gccgagcagc tcggccagcg tgcggctgtc gtcctcgtcc agggcggcga
     1321 tcgtccccga ggtgtcgtcg tcctggtcgt ggacgaagag ctcgcgccgg ccggtccgat
     1381 ggtggacgac gccgatcctg ctccccctgg acgtcaggaa ctcgtaccgg atcccgagac
     1441 ccggcagctt cgtctcctcg acttccgcca tggcttcgac ctcccgatca cagcacctcg
     1501 gtctcgttga tgacgacacg gaagcggcac tcaagctcct cggccgccct cctgcacatc
     1561 gccatccgat cgaggaagat ctcgaagtac tccatcacgt ggctgatcga cgtgtcgatc
     1621 gtcagctcca gggtgatcgt gcccgcgtcc gcgtccacgc ggaggaagga ctcctcgacg
     1681 gcgtagttca cgcgatcgtg gatgtcgaac tcgatctggt cggccttgcg gacgcgcgac
     1741 cgatgcacgt ccgacttgtc ggccaggatc acggccgcgg ccacggggcc gaccgcgtgc
     1801 ccgtactcct cctcgtggtt gccgatcgca cccatcacca ccgcgaggtc cgcgggctcg
     1861 accttgctcc ccagcaggtg gttgaccagg agcgcccccg tctgcccgtg gtccgtgcgg
     1921 gagatgcagt tcccgatgtc gtgcaggtag ccggccacgg acccgagggt cgtcgtccga
     1981 ggatcgtgcc ccaggcggtc gagcacgttg taggcgatct tgcccgtgag cttcgcgtgg
     2041 cggaacccgt gctcggtgaa gcccatcccg tccatctgct tgtcggcctg ggcgatgaac
     2101 gcggacagga cggggtgctc ctggacctcc tcgagcgaga tcgacggctc ggcgggcgcc
     2161 ttcggctccc ggcgcgacgc ggcgtcgcgc ttggcgcccg acgccgcggc ctcccgacgg
     2221 ggcccgcggt cgctcactcc tcgatgaccc cttcgtccgg ctcccccggc tccggcgtca
     2281 ggcgccgcgc gatcgcgctc ttcacgaacc gcacccgcgt gccggggctg atctcgacgg
     2341 tgacggactc gtcgtccatc tccatgatgc gaccgtacag gccgccgatc gtgatgacct
     2401 cgtccccgac cgctaggctc tccacgagct ctcgctgctg gcgcatcctg cgctgctggg
     2461 ggcggatcag caggaagtag aagatcgcca ccaggaggac gagcgagatc aggaacgtca
     2521 cgttcccctg gccgccggcc tgagcgatcg gtagggctgt catgtgtctg cctccatgaa
     2581 aaacggcggc ctcgccgccg gttgtccggc cgcgattcta tcggaccggg gcgagcgacg
     2641 caggaaggtc agacgaggcg gccggaagcc ggcatcccga ggtggcggcg ggccatctcg
     2701 gtgacgatcc ggccccgcgg ggtgcgctgg atgaagccgc tctgcaggag gtacggctcg
     2761 tagacgtcct cgagggtccc cgcgtcctcg cccacggcgg ccgacagggt ggagagcccc
     2821 accgggccgc ccccgaaccg ctcgatcatc gccgtgagga tcagcgtgtc cacgcggtcg
     2881 agcccgagct cgtccacctc gaagagggcc agggcgtcgc aggcgatctc gcggtcgatc
     2941 cgcccgtcgg cgcggacctg cgcatagtcc cggacccgct tcaggaggcg gttggcgatg
     3001 cggggggtcc cccgagagcg ggtcgcgatc tcctcggccc cctcgggatc gaccgccacc
     3061 tggaggatcc ccgccgagcg agccacgacc ttcgccaggt cctcctcgcc gtagtactcg
     3121 aggcggggcg agaacccgaa ccgctcccgc agaggcaggg tgatgagccc cgggcgggtc
     3181 gtcgcggcca cgagcgtgaa ccgaggcagg tcgagccgga tcgtgcgggc gctcggcccc
     3241 ttcccgatca ccacgtcgag cttgaactcc tcgagcgccg ggtacatgac ctcctccacc
     3301 gctcgaggca tccggtggat ctcgtccacg aacagcacct cgtgctcctc gaggttcgtg
     3361 aggatcgcgg cgaggtcggc agtccgctcc agtgcgggcc ccgaggtctg ccggagggcc
     3421 acccccatct cgttcgccag gatgccggcc agggtcgtct tccccaggcc cggggggccc
     3481 gagaacagca cgtggtccac gggctcgcgg cgctcgcggg cgccggcgat caggagcccg
     3541 agctgctcct tgatccgctc ctgccccacg aactcgtcga gggaccgggg gcggagcgcc
     3601 gcgtcgaact ccgggtcgag cgcgtcggcg tccaccagcc gctcgtcggc gaagggctcc
     3661 tcggtcatcg cttcgctccg gccgcctccc ggccgcccac ccgtcgcagc gcctcgcgca
     3721 gcagctcctc caccgggcgt ccgtcaacgt cgagctcccc cacggcgccc tggatctcct
     3781 ggggcgagag gcccatcccc tcgagggcct ggcggacctc cgccagcggg cccgcgcccg
     3841 ggagatcgat ctcgccgccg agcttgtcgc gcaggtcgag gacgatgcgg ccggccacct
     3901 tcttccccac gcccgggacg acggtgaccg cgtcgatgtc gccgtcgagc accgcccgcc
     3961 gcagggtgtc gggctccagg gtggagagca ccgccatcgc catcttcggt cccacgcccg
     4021 agaccgtgat cagcagctcg aagaggtcgc gctccgcgcc cgaggagaag ccgaacagca
     4081 ccatcgagtc gtcacggacc tggaggtggg tcaggagtcg cgagggcttc ccgaccgccg
     4141 gcagcgccgc cagcgtcgtg gtcgagacaa ggagctcgta tcccagcccg cccgcgttca
     4201 cgaccacccg gtctgcgccc cgctcggcca ggacgccctc cacgaacgcg atcaccggac
     4261 ggcctccgcc agcggcgctc ggtgcaggtg gcacagggcc acggccgcgg cgtcggcggc
     4321 gtcgggctgg cgcgggacgc cctccacccg atggatcctc gccagggagc ggcggacctg
     4381 ctgcttcccg gcgttcccgt cgccggtgat cgccat
//
LOCUS       DASUTW010000320         2923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1921915, whole genome shotgun sequence.
ACCESSION   DASUTW010000320 DASUTW010000000
VERSION     DASUTW010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1921915"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VF058_11040"
     CDS             complement(<1..357)
                     /locus_tag="VF058_11040"
                     /inference="COORDINATES: protein motif:HMM:NF013483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEX6208876.1"
                     /translation="MKRQRQQAILELVRREPLSSQRAILDRLREQGFEVTQPTVSRDL
                     DELGLARVRDDSGRLRYAAPQEAAPLGREEQLRHVLQEFALAMVASGNLVVVRTPPGA
                     ANAVAQVLDRVEVPDVI"
     gene            complement(354..1529)
                     /locus_tag="VF058_11045"
     CDS             complement(354..1529)
                     /locus_tag="VF058_11045"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HEX6208877.1"
                     /translation="MTASPPLADRAAGVLMDTYARFPVTFVRGEGIRLYDDEGREYLD
                     FAAGIAVVQIGHAHPRWVEAVTAQAARLAHVSNLFSTEPQVDLAERLVGLLGWGRAFF
                     ANSGAEANEAALKLVRKATGRSKVVATSGGFHGRTLATLAATGQPEKHAPFEPMPREF
                     VHVPFGDPEALRDAVDERTAAVLLEPVQGEAGVVPAPEGYLAAARGACDGAGALLVLD
                     EVQTGVGRCGAWFAHQLEDLEPDVVTLAKGLAGGLPIGVCLATPEVSAAFGPGDHAST
                     FGGGPIPCAAALAVLEVIEEEGLLERARVMGERLVEALRETAASAVEVRGRGLLVGVE
                     LPAEISTREVVTAALGRRLVLTQAGRNTVRFTPPLIATADDVGEAAHIFGAVLEEET"
     gene            complement(1526..2332)
                     /gene="argB"
                     /locus_tag="VF058_11050"
     CDS             complement(1526..2332)
                     /gene="argB"
                     /locus_tag="VF058_11050"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX6208878.1"
                     /translation="MREHSGAVIVVKLGGSAMSDPGLLETFALDVALLRQVGVRPVIV
                     HGGGPQITRASERLGLTPRFVRGLRVTDAETLEVVRTVLVGRINKDLVKAMNERGAPA
                     AGVSGEDADLMIATPRSEDLGLVGSITEVRPALLASLMEDFVPVVASLATDGAGQSLN
                     VNADEAAAALAVALGASKLVYLTDVPGIIEDRGGDPALVSEASVAECERLLSSGAVTD
                     GMIPKIEHLVHALRGGVQRGHVLDGRVPHAMILELFTPEGLGTMVTRGEA"
     gene            complement(2407..>2923)
                     /locus_tag="VF058_11055"
     CDS             complement(2407..>2923)
                     /locus_tag="VF058_11055"
                     /inference="COORDINATES: protein motif:HMM:NF014065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEX6208879.1"
                     /translation="CASTNDTVLVMASGAARPASPEPLGAALREASLDLARKVVAEAE
                     GASKTLVVHVSGAADDEQARHAGRAVAGSLLVKTALFGEDPNAGRLLQAIGAAGVSLD
                     PSAADVDLAGHRVVEGGVVAAFDQRACREALKEREVVVRVALGEGEGEATFFGCDLGY
                     EYVRINAEYRT"
BASE COUNT          391 a         1131 c         1030 g          371 t
ORIGIN      
        1 gatcacgtcg ggcacctcga cccggtcgag cacctgggcc acggcgttcg ccgcccccgg
       61 cggcgtacgg acgacgacga ggttgcccga ggccaccatg gcgagcgcga actcctggag
      121 gacgtgccgg agctgctcct cgcgcccgag cggcgcggcc tcctggggcg ccgcgtagcg
      181 gaggcgcccg gagtcgtctc ggacccttgc cagccccagc tcgtcgaggt ccctggagac
      241 cgtgggctgc gtgacctcga acccctgctc ccgcaggcga tccaggatcg cccgctggga
      301 gctgagcggt tcccggcgca ccagctccag gatcgcctgc tgccgctgcc tcttcatgtc
      361 tcctcctcca ggaccgcgcc gaagatgtga gcggcctcgc ccacgtcgtc ggccgtcgcg
      421 atgaggggcg gagtgaagcg caccgtgttg cgacccgcct gggtcagcac aagcctccgc
      481 cccagggccg cggtgacgac ctcgcgggtg gagatctcgg cggggagctc tacgccgacc
      541 agcaggcccc ggccccgcac ctcgaccgcc gacgcggccg tctcgcggag cgcctccacc
      601 agccgctccc ccatgacccg ggcccgctcc agcaggccct cctcctcgat cacttcgagc
      661 accgcgaggg cggcggcgca cgggatcggc ccgccgccga aggtcgaggc gtgatccccc
      721 ggcccgaagg ccgccgagac ctccggggtc gccaggcaga cgccgatcgg gaggccgccg
      781 gccagcccct tggccagcgt gacgacgtcg ggttccaggt cctcgagctg gtgggcgaac
      841 cacgccccgc agcggccgac gccggtctgc acctcgtcca ggacgagcag ggcccccgcc
      901 ccgtcgcagg cgcctcgggc cgccgccagg tagccctcgg gcgcgggcac gacgccggcc
      961 tccccctgca ccggctcaag cagcacggcg gccgtccgct catcgacggc gtctctcagc
     1021 gcctcggggt cgccgaacgg cacgtggacg aactcgcggg gcatcggctc gaacggggcg
     1081 tgcttctcgg gctggcccgt ggccgccagc gtggccaggg tccggccgtg gaaccccccg
     1141 ctcgtcgcca cgaccttgga ccggccggtc gccttgcgga cgagcttgag ggccgcctcg
     1201 ttcgcctcgg ctcccgagtt ggcgaagaac gccctccccc atccgaggag cccgacgagc
     1261 cgctcggcca ggtccacctg gggctcggtg gagaagaggt tgctgacgtg cgccagacgg
     1321 gcggcctggg ccgtcaccgc ctcgacccac cgagggtggg cgtggccgat ctggaccacg
     1381 gcgatgcccg cggcgaagtc caggtactcg cgaccctcat cgtcgtacag ccggatcccc
     1441 tcgccacgca cgaacgtcac ggggaaccgg gcgtacgtgt ccatcagaac gccggcggct
     1501 cggtcggcga gcggtggcga ggccgtcacg cctcccccct cgtcaccatc gtgcccaggc
     1561 cctcgggcgt gaacagctcg aggatcatcg cgtgggggac ccgcccgtcg aggacgtggc
     1621 cccgctggac gccccctcgg agggcgtgga ccagatgctc gatcttgggg atcatgccgt
     1681 ccgtcaccgc gcccgaggag aggaggcgct cgcactcggc caccgacgcc tccgagacca
     1741 gcgccgggtc tccccctcga tcctcgatga tccccgggac gtcggtcagg tagacgagct
     1801 tcgaggcccc gagcgcgacg gcgagcgcgg cggcggcctc gtcggcgttg acgttgagcg
     1861 actggccggc gccgtcggtg gcgagcgagg ccacgaccgg gacgaagtcc tccatcagcg
     1921 acgcgagaag ggcgggccgg acctcggtga tcgagcccac cagcccgagg tcctcgcttc
     1981 gcggcgtggc gatcatgaga tctgcgtcct cgcccgagac gcccgcggcc ggggccccac
     2041 gttcgttcat cgccttcacc aggtccttgt tgatgcgccc aacgaggacc gtccgcacga
     2101 cctcgagggt ctcggcgtcg gtgacgcgga gcccccggac gaaccggggg gtgagcccca
     2161 ggcgctcgga cgcccgcgtg atctgcgggc cgccaccgtg gacgatcacc gggcggaccc
     2221 ccacctggcg gagcagggcc acgtcgaggg cgaaggtctc caggaggccg gggtcgctca
     2281 tcgccgaccc tccgagcttg acgacgatca ccgcgccgct gtgctcacgc atgtagggga
     2341 gggcctcgag cagcacgcgg cccttggcca cggtctcctc gcggggccgg gtcccggcgg
     2401 cgcggctcac gtccggtact ccgcgttgat ccgcacgtac tcgtacccga ggtcgcagcc
     2461 gaagaaggtg gcctcgccct cgccctcgcc gagggcgacc cggaccacca cctctcgctc
     2521 cttgagggcc tcccgacacg cccgctggtc gaacgcagcc accacaccgc cctccaccac
     2581 ccgatggccg gcgaggtcaa cgtccgcggc cgaggggtcg agcgacacgc cggccgcgcc
     2641 gatcgcctgg aggagacgcc ccgcgttggg gtcctcgccg aacagggccg tcttgaccag
     2701 cagcgagccg gcgacggcgc ggccggcgtg tcgcgcctgc tcgtcgtcgg cggcgcccga
     2761 gacgtgaacg acgagcgtct tgctcgcgcc ctcggcctcc gccacgacct tgcgggccag
     2821 gtcgagcgag gcctcgcgca gcgccgcccc cagcggttcg ggagacgcgg gtctcgccgc
     2881 gccgctcgcc atgacgagca ccgtgtcgtt ggtgctggcg cag
//
LOCUS       DASUTW010000321         7225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1145028, whole genome shotgun sequence.
ACCESSION   DASUTW010000321 DASUTW010000000
VERSION     DASUTW010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7225
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145028"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VF058_11060"
     CDS             <1..753
                     /locus_tag="VF058_11060"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX6208880.1"
                     /translation="NSSAFRQDPGVPLVIPEINAHALRDHDGLIANPNCTATTALMAV
                     APLNRAAGVRAMVASAYQSVSGTGRRAMQELLDQTRKTIDQVEGLRGQEELDGPGPEV
                     YPHPIAFNVFPQCETFPAGADTSTEEVKMEAESRKILEHPDLIAHATSVRVPVIVGHA
                     VSVSLSLGRPMEPQEARELLAAFPGVRVVDDPERAEYPTPQAAAGIDDTLVGRIRANP
                     AMEHGLSLFVCQDNLRKGAALNNVHIAEALGS"
     gene            750..1193
                     /locus_tag="VF058_11065"
     CDS             750..1193
                     /locus_tag="VF058_11065"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX6208881.1"
                     /translation="MSDQRTEPVYEIDLRDLADEGAGPVVIPDADPDPAPETVTLPTE
                     GEALAGDPPPGDVPALLEMPAHPRPARRRIACERLGYQRARAAVDPLLERLQDPERGV
                     RIKAADALALIGDERAIEPLERARAADPDLGVAQAMERAARTLAG"
     gene            complement(1195..2310)
                     /locus_tag="VF058_11070"
     CDS             complement(1195..2310)
                     /locus_tag="VF058_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX6208882.1"
                     /translation="MFPLSKGRVAKQAHVGLPDGTYEEEHGREAFDGRASHLYRSHPP
                     TAWVRLDGPLRPRAYDLSGLKTPDQADADAGWQRVLFNEDVAIYVSRPTGAPSWYLRD
                     ADGDLCYFVHRGTGRVETDYGPLRFRDGDYLIVPKGTTHRVIPEGDDTYLYVIEAAGE
                     YHLPDKGILGKHALFDPGVIETPEPDPRDEEGEFEVRMKRDGAFTTLVYPFHPLDVVG
                     WQGDLCPLRVNVEDFRPVVSPRYHLPPSVHVTWETEGFEIGTFAPRPTETGDPTALRV
                     PFFHSNIDRDEVLFYHRGQFFSRAGIGEAFITHHPQGLHHGPQAAAMEAGKTKEFADE
                     YAIMVETLRPLQRDEAIAAVEDEGYATSWARGLGLIE"
     gene            complement(2442..3269)
                     /gene="proC"
                     /locus_tag="VF058_11075"
     CDS             complement(2442..3269)
                     /gene="proC"
                     /locus_tag="VF058_11075"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX6208883.1"
                     /translation="MSDRRVAILGGGKMGEALLSGLLRSGRSPEDIMVTVRREERAAE
                     LAETYGVQTALDSAEAVGWAKVLVLSVKPQDMEALLEQIAPAVTEDHVIVSVAAGIRC
                     ALIERMTPGDVPVVRVMSNVPVHVDEAMSAISPGAHAEDKDLAIAEELLASVGKVIRL
                     PEKHQDAVTATSGSGPAYFALLAEAMIDACILLGLSRDVATELIVQTMIGSAKMLRDM
                     NSHPVELREMVTSPGGTTIAAIRELENAGVRAAFLNAIEAARQRSVELAKGQDAEEV"
     gene            complement(3271..3810)
                     /locus_tag="VF058_11080"
     CDS             complement(3271..3810)
                     /locus_tag="VF058_11080"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX6208884.1"
                     /translation="MAGRALIVVDTQKGFTTMGNLASERTTAAVPRIREIVAEEHAAG
                     TPIVFTKDSHVENDLEFEMFPPHCIIGTEEQELAPELQEFEADAAAVIEKRRYSAFFD
                     TDLEQLMAKLAPDEVHVIGVCTDICVLHTTADLRNRDYRVVIHKDGVETFDAPGHDGD
                     EVNRFALAHAEHILGAKVV"
     gene            complement(4231..4707)
                     /locus_tag="VF058_11085"
     CDS             complement(4231..4707)
                     /locus_tag="VF058_11085"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX6208885.1"
                     /translation="MSDEIAVRILAFLRERAQEDELTLLASSDRAWDVLLPSYWKETL
                     AVSLELGDWNLRGESFFMRAPEENRAETFHLLLQRNRRPGPWRFAANEEGDVSLLASI
                     PRGAVTEEELDRLLGSLVQVTDETYVPAMKLGYERGLAEQVERGGPGLDQPPPWAR"
     gene            complement(4704..5966)
                     /locus_tag="VF058_11090"
     CDS             complement(4704..5966)
                     /locus_tag="VF058_11090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017976946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6208886.1"
                     /translation="MRAPGAARARVAMISLHTSPLDPPGTGDSGGMNVHVRETMRRLA
                     DRDIAVDVYTRCAGRGVPEVHELTRLTRVIQVQAGPCAVVDKRDLPELVPDFVRSFLG
                     RDLPPYDVIHAHYWLSGQAARAARSAWGAPLVASFHTLGRVKNAALAPGEPPEPEARI
                     RGEEAMIRSADRVVAPTEIEARDLVARYRARSDSIRVVHPGVDRSRFRPRPKDASKMA
                     WGLEGSRVALFVGRLQPHKGPDVAIRTVAEAIRADPHATHDLVLVLAGGSSGGWGPGD
                     LQALARAEGIADRVRFVDSVIHEELPSLYAAADVLLVPSRTESFGLAALEAQACGVPV
                     VAASVGGLRLVVGDGESGFLVPGHDPREHAERLLRVLRSPQLAMRLSAGAEDRAERFP
                     WDRTVDDLLDVYGELAPALADPDAAVAP"
     gene            complement(6058..6369)
                     /locus_tag="VF058_11095"
     CDS             complement(6058..6369)
                     /locus_tag="VF058_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208887.1"
                     /translation="MIEQMQNAGYALIGLGDLAAEKVKEMVDRARKIKVDREEIESTY
                     GDLSKRGTKIVRKVTTSKPAKQAKEGTKQAARQLKGAATSLRKAAGREEEKKARAKKA
                     S"
     gene            complement(6366..6740)
                     /locus_tag="VF058_11100"
     CDS             complement(6366..6740)
                     /locus_tag="VF058_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016472265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6208888.1"
                     /translation="MRTSVGRFIRDQRRQAQLSLRRLAQLAGVSNPYLSQIERGLKKP
                     SAEILQGIAKALRISAETLYVKAGILESEKAGDTVSAIMTDAALTERQKQALVQIYES
                     FRSETQARREARRAKTQRKEQS"
     gene            complement(6821..>7225)
                     /locus_tag="VF058_11105"
     CDS             complement(6821..>7225)
                     /locus_tag="VF058_11105"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX6208889.1"
                     /translation="GAPAAALPLRAIATAFARLPQELGPSAARVVEAMRAEPYLVAGR
                     GRVCTAVMEAFPVVAKVGADGVAGASLLGRGLGIALKVDDGAGRARDAALVRALALLD
                     AVDPSLVPAFAAPPVLGGGTPVGELRSDFSVA"
BASE COUNT          989 a         2675 c         2500 g         1061 t
ORIGIN      
        1 aactcctcgg cgttccggca ggacccgggc gtcccgctcg tgatccccga gatcaacgcc
       61 cacgcgctcc gggaccacga cggcctgatc gccaacccga actgcaccgc gacgaccgcg
      121 ctgatggccg tggccccgct caaccgggcc gcaggcgtcc gggccatggt ggcctcggcc
      181 tatcagtcgg tctcgggcac cggtcgccgg gcgatgcagg agctgctcga ccagacccgc
      241 aagacgatcg accaggtgga ggggctgcgg ggtcaggagg agctcgacgg gcccgggccc
      301 gaggtctacc cgcacccgat tgccttcaac gtcttcccgc agtgcgagac gttccccgcg
      361 ggggccgaca cctccaccga ggaggtcaag atggaggcgg agagccgcaa gatcctcgag
      421 catccggacc tgatcgctca cgccacgtcg gtccgcgtac cggtgatcgt cggccacgcc
      481 gtctcggtct cgctctcgct ggggcggccg atggagccgc aggaggcccg cgagctgctc
      541 gccgccttcc cgggggtccg ggtggtggac gaccccgagc gggccgagta cccgaccccg
      601 caggcggccg cgggcatcga cgacaccctc gtgggccgga tccgggccaa cccggccatg
      661 gagcacggcc tctcgctctt cgtctgccag gacaacctcc gcaagggcgc cgcgctcaac
      721 aacgtgcaca tcgccgaagc cctggggtcg tgagcgacca gcgcactgag cccgtctacg
      781 agatcgacct gcgggacctg gccgacgagg gcgccggccc cgtcgtgatc ccagacgcgg
      841 acccggaccc ggcccccgag acggtgacgc tgccgaccga gggggaggcg ctcgcaggcg
      901 atcctccgcc cggcgacgtc cccgccctcc tcgagatgcc cgcccacccc cgtcccgccc
      961 ggcggaggat cgcctgcgag cgcctcggct accagcgggc ccgggccgca gtggatccgc
     1021 tcctcgagcg cctccaggac cccgagcgcg gcgtccggat caaggccgcg gacgcgctgg
     1081 cgctcatcgg ggacgagcgg gcgatcgagc ccctggagag ggcccgtgcc gccgaccccg
     1141 atctgggcgt ggcccaggcc atggagcggg ccgcccggac gctcgccggc tgagctactc
     1201 gatcaggccg agcccccggg cccaggacgt ggcgtagccc tcgtcctcga cggccgcgat
     1261 cgcctcgtcg cgctgcagcg gtcgcagggt ctcgaccatg atcgcgtact cgtcggcgaa
     1321 ctccttcgtc ttgccggcct ccatcgccgc cgcctgtggg ccgtgatgca ggccctgcgg
     1381 gtggtgggtg atgaacgcct cgccgatccc cgcccgcgag aagaactgcc cgcggtggta
     1441 gaagagcacc tcgtcccggt cgatgttcga gtggaagaac ggcacgcgca gcgccgtggg
     1501 gtcgcccgtc tcggtgggcc tgggcgcgaa ggtcccgatc tcgaacccct ccgtctccca
     1561 cgtcacgtgg accgacggtg gaaggtggta gcggggcgag accaccggcc ggaagtcctc
     1621 gacgttgacg cgcagcgggc agaggtcgcc ttgccacccc acgacgtcga gcgggtggaa
     1681 cgggtagacg agcgtggtga aggcgccgtc gcgcttcatc cggacctcga actcgccctc
     1741 ctcatcgcgg gggtcaggct ccggcgtctc gatcacgccg gggtcgaaca gcgcgtgctt
     1801 ccccaggatc cccttgtcgg ggaggtggta ctctcctgcg gcctcgatca cgtagaggta
     1861 cgtgtcgtcg ccctcgggga tcacgcggtg cgtcgtcccc ttggggacga tgaggtagtc
     1921 gccgtcccgg aaccgcaggg ggccgtagtc cgtctccacc ctcccggtcc cccggtggac
     1981 gaagtagcag aggtcgccgt cggcgtcccg caggtaccag gacggcgcgc cggtgggtcg
     2041 cgacacgtag atcgcgacgt cctcgttgaa gaggacgcgc tgccagccgg cgtctgcgtc
     2101 ggcctgatcc ggcgtcttaa gaccgctgag gtcgtaggcg cggggccgca gcggcccgtc
     2161 cagcctgacc cacgccgtgg gcgggtgcga acggtacagg tgggaggccc ggccgtcgaa
     2221 cgcctcacgg ccgtgctcct cctcgtaggt gccgtcgggg aggccgacgt gcgcctgctt
     2281 ggccacgcga cccttcgaga gtgggaacat cggatccctc ctcagctctt ggcggccttc
     2341 cgggcctcgc ccttctcctg cgccttccgc cggtcgcgct cccgcttctt ccgctcgcgg
     2401 tcgtgcgccc atcgcacctt cctgcggtct cccctgccca ttcagacctc ctcggcgtcc
     2461 tgtcccttgg ccagctccac actgcgctgg cgcgccgcct cgatcgcgtt gaggaacgcc
     2521 gcgcgcacgc ccgcgttctc cagctcgcgg atcgcggcga tcgtcgtgcc tcccggcgag
     2581 gtcaccatct cgcgcaactc caccgggtgc gagttcatgt cgcgcagcat cttggccgac
     2641 ccgatcatcg tctgcacgat cagctcggtg gccacgtcgc gcgagagccc caggaggatg
     2701 caggcgtcga tcatcgcctc ggccaacagg gcgaagtagg cgggccccga gcccgaggtc
     2761 gccgtgacgg cgtcctggtg cttctcgggc agccggatca ccttgcccac cgaggccagc
     2821 agctcctcgg cgatcgccag gtccttgtcc tcggcgtggg cgcccgggct gatcgcagac
     2881 atcgcctcgt ccacgtgaac ggggacgttc gacatcaccc ggaccaccgg cacgtctccg
     2941 ggcgtcatcc gctcgatcag cgcgcaccgg atcccggccg ccacgctgac gatcacgtgg
     3001 tcctcggtga ccgcgggggc gatctgctcg agcagggctt ccatgtcctg gggcttgacg
     3061 gagaggacga gcaccttcgc ccagcccacg gcctccgcgc tgtcgagcgc ggtctggacg
     3121 ccgtaggtct cggcgagctc cgcggcccgc tcctcgcgtc gcaccgtcac catgatgtcc
     3181 tcggggctgc ggcccgaccg gaggagcccc gagaggagcg cctcccccat cttcccgccg
     3241 cccaggatcg ccacccgccg gtcgctcatc tcacaccacc ttcgcgccga ggatgtgttc
     3301 tgcgtgagcc agggcgaagc ggttgacctc gtccccgtcg tggccgggcg cgtcgaacgt
     3361 ctccacgccg tccttgtgga tcacgacgcg gtagtcccgg ttccgcaggt cggcggtcgt
     3421 gtggaggacg cagatgtcgg tgcagacgcc gatcacgtgg acctcgtccg gcgccagctt
     3481 ggccatcagc tgctcgaggt cggtgtcgaa gaaggccgag tagcgccgct tctcgatcac
     3541 cgcggccgcg tccgcctcga actcctggag ctcgggggcg agctcctgct cctcggtccc
     3601 gatgatgcag tgcggaggga acatctcgaa ctcgaggtcg ttctcgacgt ggctgtcctt
     3661 cgtgaagacg atgggcgttc ccgcggcgtg ctcctcggcc acgatctcac ggatcctggg
     3721 gacggccgcg gtggtgcgtt cgctggcgag gttccccatc gtggtgaacc ccttctgcgt
     3781 gtcgacgacg atgagcgcgc ggcctgccat cggaaccctc ctgggtcgag atggccacag
     3841 tataggaaaa gcccgaggga cctccggctc gccgtcccga ccctcgagcc gaaactcgat
     3901 gatcgcgaca gccaccttca gcccctcggg cttagcgctc cgagcaacct acccgttcgc
     3961 tccggtaccc cggcgcggta gcccctttcg ttgggccttg gggccttccg agcctcccca
     4021 cccctacagc tagttggcct tcccaaccgc aggagcaggt gcgcccagtt ggccttacag
     4081 ctttcccagg gggccccgct cggagcccga tccgagcggt tcagctgctc ggagctagcc
     4141 ggcaatctag cgacgggggg ccaggccgtc aaggtgccaa aaccccaggt cgccacccgt
     4201 ttccgcaggt cagcccggaa gaaaaaaaga tcagcgggcc cagggagggg gctggtcgag
     4261 gcccgggccc ccgcgctcca cctgctcagc cagcccgcgc tcgtacccca gcttcatcgc
     4321 ggggacgtag gtctcgtcgg tgacctggac cagcgagccg agcaggcgat cgagctcctc
     4381 ctcggtgacg gcgccccggg ggatcgaggc gaggaggctg acgtcgccct cctcgttcgc
     4441 ggcgaaccgc caggggcccg gccggcggtt ccgctgcagg agcaggtgga acgtctccgc
     4501 ccgattctcc tcgggcgctc gcatgaagaa cgactcgccc cggaggttcc agtcgcccag
     4561 ctccaggctc accgccaggg tctccttcca gtacgagggc agcaggacgt cccaggcccg
     4621 gtcgctggac gccaggagcg tcagctcgtc ttcctgggcg cgctcccgga ggaaggcgag
     4681 gatccggacg gcgatctcgt cgctcacggc gccaccgctg cgtccgggtc ggccagggcg
     4741 ggcgcgagct ccccgtagac gtcgaggaga tcgtccacgg tgcggtccca ggggaacctc
     4801 tcggcccgat cctcggcgcc ggccgacaag cgcatcgcga gctgggggga tcgaaggacg
     4861 cggagcaggc gctctgcgtg ctcgcgcggg tcgtgccccg gcacgaggaa gccgctctcg
     4921 ccgtcgccga cgacgaggcg aagacctccg accgacgccg cgacgaccgg cacgccgcag
     4981 gcctgggcct cgagtgcagc gaggccgaag gactcggtgc gcgaggggac gaggaggacg
     5041 tcggccgcgg cgtacagcga gggcagctcc tcgtggatca cgctgtccac gaagcgcacc
     5101 cggtccgcga tcccctccgc gcgggccagc gcctggagat caccggggcc ccagccaccg
     5161 ctcgagccgc cggccaggac cagcacgagg tcgtgcgtcg cgtggggatc ggctcggatc
     5221 gcctcggcca ccgtgcggat cgcgacgtcg ggccccttgt gcggttggag acggcccacg
     5281 aagagcgcca cccggctgcc ctcgaggccc caggccattt tggacgcgtc cttgggccga
     5341 ggacggaacc gggagcggtc cacgccggga tgcacgacgc ggatcgagtc ggaccgggct
     5401 cggtaccggg cgacgaggtc gcgggcctcg atctcggtgg gcgccacgac gcggtcggcc
     5461 gagcggatca tggcctcctc gccgcggatg cgggcctccg gctcgggggg ttcccccggg
     5521 gcgagggcgg cgttcttcac gcgcccgagc gtgtggaacg aggccacgag cggcgccccc
     5581 cacgccgatc gggcggcgcg ggcggcctgg cccgacagcc agtagtgcgc gtggatcacg
     5641 tcgtagggcg ggaggtcgcg ccccaggaac gagcggacga agtcggggac gagctcgggg
     5701 agatcgcgct tgtccaccac ggcacacggt cccgcctgga cctggatcac gcgggtgagc
     5761 cgcgtgagct cgtgcacctc cgggaccccg cgcccggcgc agcgcgtgta gacgtccacg
     5821 gcaatgtccc gatcggccag gcggcgcatc gtctcgcgga cgtggacgtt catcccgccc
     5881 gagtcgcccg tgcccggcgg atccagcggg gacgtgtgga gggagatcat cgcgacccgc
     5941 gctcgggccg ccccgggggc tctcatcggg acaatcctag gccagcggtc gcgttccgac
     6001 acggggcggc cgccgcgcgc gttcgcgcgc ggcggccgcc gcgatccccg ctgggcctca
     6061 gctggccttc ttggcccgag ccttcttctc ctcctcccgg cccgcggcct tccggaggct
     6121 ggtcgcggcg cccttcagct gacgggccgc ctgcttcgtg ccctccttgg cctgcttcgc
     6181 cggcttcgag gtcgtgacct tgcgcacgat cttcgtgcca cgcttgctga ggtcgccgta
     6241 ggtggattcg atctcctccc ggtcgacctt gatcttccgg gcgcggtcca ccatctcctt
     6301 gaccttctcg gcggccaggt cgcccaggcc gatcagcgcg taccccgcgt tctgcatctg
     6361 ctcgatcatg actgctcctt cctctgcgtc ttcgcgcgcc gcgcctcgcg acgcgcctgc
     6421 gtctcggatc ggaagctctc gtagatctgg acgagggcct gcttctgccg ctcggtgagg
     6481 gccgcgtcgg tcatgatcgc gctcacggtg tccccggcct tctcggactc aaggatcccg
     6541 gccttcacgt acagtgtctc ggcggagatc cgcagcgcct tggcgatccc ctggaggatc
     6601 tcggcgctcg gcttcttcag gccccgctcg atctgcgaga ggtacgggtt ggagacaccg
     6661 gccagctggg ccagcctccg cagggagagc tgcgcctgcc ggcgctggtc cctgatgaac
     6721 cgtcccacgc tcgtccgcat ctccagagct ccttccacct gcaccctatc acggaaatgc
     6781 taactatggc aagcagagcg ggaaccactg gcaagcaccc tcaggcgacg gagaagtccg
     6841 agcggagctc ccccaccggg gtcccgcccc cgagaaccgg cggggctgcg aaggcgggca
     6901 cgagggacgg gtccaccgcg tcgagcaggg ccagggcccg gacgagcgcc gcgtcgcgag
     6961 ctcgccccgc gccgtcgtcg accttgagcg cgatcccaag accgcggccc aggagggagg
     7021 ccccggccac gccgtcggcc cccaccttcg ccaccaccgg gaaggcctcc atcacggccg
     7081 tgcacaccct cccccgaccc gcgacgaggt agggctcggc ccgcatcgcc tcgaccaccc
     7141 gggccgcgga gggccccagc tcctggggga ggcgggcgaa ggccgtggcg atcgcgcgca
     7201 gcgggagggc ggcggccggc gcgcc
//
LOCUS       DASUTW010000322         6936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1403126, whole genome shotgun sequence.
ACCESSION   DASUTW010000322 DASUTW010000000
VERSION     DASUTW010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403126"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="VF058_11110"
     CDS             complement(<1..1022)
                     /locus_tag="VF058_11110"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEX6208890.1"
                     /translation="MGDDERRGRWQGFDFGGGSGDGRRWRFPIGYLLLAFLLLIFLQS
                     LFVRSTDEISYSRFLAVVQEDRIEGLVRISSGSIEGEFQNQDGDVERFSVIRPPDVQD
                     EQLIGLLQENGVEFTGEQPSGFVNFLAVWGPFILIFGLMFLFFRRMAGGGAGSALNLG
                     KKGVKIYDRKDLKTTFDDVAGLDEAIEELREVVDFLANPKKYQRLGGRIPKGVLLMGP
                     PGCGKTLLARAVAGEAGVPFFYMSGSDFVEMFVGLGAARVRELFQQAKEKAPVLIFID
                     EIDTLGKSRSGAMGGSLGSHDEREQTLTQLLSEMDGFDTSKGVIIMAATNRPDVLDPA
                     LLRPGR"
     gene            1085..1708
                     /locus_tag="VF058_11115"
     CDS             1085..1708
                     /locus_tag="VF058_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6208891.1"
                     /translation="MPDRETPSEPPRSVTTVRTDASREELDRRFELEAIPLLEEMYGA
                     ALRLTRNPADAQDLLQETYLRAFRGFAGFEEGTNLKAWLYRILTNSYINLYRKRQREP
                     QTVSDDEVEEWYLYSRLRESDLEPSAEAEVIERLPDEDVQAALASLPDVYRMAVILAD
                     IEGFSYKEIAAILDVPIGTVMSRLHRGRRALERALWDIVRERGVVRD"
     gene            1695..1949
                     /locus_tag="VF058_11120"
     CDS             1695..1949
                     /locus_tag="VF058_11120"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6208892.1"
                     /translation="MSGIECEEILRQVEVYLDGELPAEEAARVEGHLAGCSPCLERQE
                     FVSNLRRILRDKCGRVERLPDAARERIHRAVAREATSETD"
     gene            2041..2202
                     /locus_tag="VF058_11125"
     CDS             2041..2202
                     /locus_tag="VF058_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208893.1"
                     /translation="MPRPSKLEAAGRVCAAPGCETGLSIYNAKSTCWQHTDLTFPNFR
                     GKRLAKGSA"
     gene            2297..2506
                     /locus_tag="VF058_11130"
     CDS             2297..2506
                     /locus_tag="VF058_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6208894.1"
                     /translation="MTNGSANAEYLKTAEVAEILHVSPKTVTRWAKDGKLPHSRTLGG
                     HRRFPAEAIRRLAEGLVQVDEIETP"
     gene            2538..2723
                     /locus_tag="VF058_11135"
     CDS             2538..2723
                     /locus_tag="VF058_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208895.1"
                     /translation="MQILLGLVILQFGLGLVLAGVAGRVRQDAGGSFWAGVALSALLG
                     LVGFWIVVASARRASRS"
     gene            2720..3460
                     /locus_tag="VF058_11140"
     CDS             2720..3460
                     /locus_tag="VF058_11140"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEX6208896.1"
                     /translation="MSGARRLALLGSGEFEPWAEEVDRWLLAGASDGRVLVAPAASAA
                     EGEEVFSRWGRLGVEHYGRLGYAAEVLPLRTRDDASDPRLASRLDEASMVFFSGGNPA
                     ALAATLAGTPFWDRLLRRLGEGLAFGGCSAGAAFLGELAPDSSIADPLEGDPWRPGVK
                     AFGGVVLAPHWDTVDSHVPGFRAAVERMAGPGLVLVGVDERTALLGDGRSWRVTGEGS
                     VHVRLQDAWTSHPPGGTLPPGVLEVAPA"
     gene            3638..4864
                     /locus_tag="VF058_11145"
     CDS             3638..4864
                     /locus_tag="VF058_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEX6208897.1"
                     /translation="MAAITDTDIDLHAPLGAYLRLRERGPSFLLESVERGRLGRHSFV
                     GTGSRLLSFEEAEAEVARGASVVGYLGYDHAARLEPVPLPVEGPGPPESLFVAAEALV
                     RFDHVLGRAEVLVGDAAEIGRALARPVPPSPRSPGLPGPIRRSPSADAYREAVRAAKE
                     HIRAGDVLQIVLSQRAERPTSASAVALYRALRRISPSPYLFLLELGDLALVGASPETL
                     VKLEGRKASANPIAGTTARGLGDAQRLLASAKDRAEHVMLVDLARNDLSRVCRPGSVV
                     LERFLDVEPYSHVTHLVSEVVGELEPSASGFDLLRACFPAGTVSGAPKVRAMQLISEL
                     EGVRRGPYGGAVGYALPDGSLDACIAIRMIVLVHGVARLQAGAGIVAASDPDLEHREC
                     LQKLAAMEQAIEEAER"
     gene            4861..5421
                     /locus_tag="VF058_11150"
     CDS             4861..5421
                     /locus_tag="VF058_11150"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX6208898.1"
                     /translation="MILLLDNYDSFTHNLAHLLRVLGVEVLVVRNDAITPEEAEAAAP
                     SHLVISPGPGCPAEAGATRAVLRRLAARTPTLGVCLGHQAIVEVFGGEVGPARRLVHG
                     EGASIRHDGRGVFRGVPQGFVAGRYHSLAARRLPDALEVSARAEDGEVMGVRHRSLPV
                     EGVQFHPESVLTPLGPSLVRNFLERR"
     gene            5427..6455
                     /gene="trpD"
                     /locus_tag="VF058_11155"
     CDS             5427..6455
                     /gene="trpD"
                     /locus_tag="VF058_11155"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX6208899.1"
                     /translation="MSITDALARLLEGSDLSREEARAVTGEIMRGEATPAQIGGFLVG
                     LRMKGETPDEIAGCAEAMRDHVLPVSPRRSDLIDTAGTGGDGAGTLNLSTAAGLVAAA
                     AGAGVAKHGNRAVSSASGSADVLEALGFSLDLSPERIAESIDEHGFGFLFAPAHHPAM
                     RHAGPVRRELAARTVFNVLGPLTNPAGARAQLVGVYARELAGTVARVLALLGADRALV
                     VHGAGGIDELSPAGPNLVFEVEEGRVEERMVDPADLGIDGCAPGDLRGGTPEENAAAI
                     REVFDGAAGPKRDAVLLNAAAALRIAGLVSDLREGLAAAAEVVDGGAAAAKLETLVAF
                     SRAEEAVA"
     gene            6452..>6936
                     /locus_tag="VF058_11160"
     CDS             6452..>6936
                     /locus_tag="VF058_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase TrpC"
                     /protein_id="HEX6208900.1"
                     /translation="MRRFRQALAAPGLSAIAEIKRRSPSAGDLRPDADVSRLARAFER
                     TGAAAVSILVDERFAGSLKDLRAARAATDLPLLAKGFFRTEEDLEEVRRAGADAALLL
                     LRDLDDRAARRLMARAEAIGLETLVEAHDGDELGRATRLGADPVGINARDLSTFRVDR
                     AR"
BASE COUNT          918 a         2435 c         2597 g          986 t
ORIGIN      
        1 aagcggccgg gccgcagcag cgcggggtcc agcacgtcgg ggcggttcgt cgccgccatg
       61 atgatgaccc ccttcgacgt gtcgaacccg tccatctccg agagcagctg agtcagggtc
      121 tgctcgcgct cgtcgtgcga cccgaggctc ccgcccatcg cgcccgagcg gctcttcccc
      181 agggtgtcga tctcgtcgat gaagatcagc accggggcct tctccttcgc ctgctggaac
      241 agctcgcgga cgcgcgcggc cccgaggccc acgaacatct ccacgaagtc ggaccccgac
      301 atgtagaaga acggcacgcc ggcctcgccg gccaccgccc gcgccagcag ggtcttcccg
      361 cagccgggag gacccatcag caggacgccc ttggggatgc gcccgccgag ccgctggtac
      421 ttcttggggt tggccaggaa gtccacgacc tcgcggagct cctcgatcgc ctcgtcgagc
      481 cccgccacgt cgtcgaaagt ggtcttgaga tccttccggt cgtagatctt cacgcccttc
      541 ttcccgaggt tgagcgcgct gcccgcgccg cccccggcca tgcgtcggaa gaagaggaac
      601 atcagcccga agatcaggat gaagggcccc cagacggcca ggaagttcac gaacccgctc
      661 ggctgctcgc ccgtgaactc gacgccgttc tcctgcagca gaccgatcag ctgctcgtcc
      721 tggacgtccg gcgggcggat gacgctgaac cgctcgacgt cgccgtcctg gttctggaat
      781 tcgccctcga tggacccgct cgagatgcgc acgagccctt caatgcggtc ctcctgaacc
      841 accgcgagga accgcgagta ggagatctcg tcggtcgacc gcacgaacag gctctggagg
      901 aagatgagaa ggaggaacgc gagcagcagg tatccgatcg ggaagcgcca gcgacggccg
      961 tccccgctcc cgccgccgaa gtcgaagccc tgccagcggc cgcgtcgctc gtcgtcaccc
     1021 atgtcccgac catcttccca cacgggccgg tggacgaacg ctccgggcgg gtatgcttgg
     1081 ccgcatgccc gatcgggaga ccccctcgga gccaccacgg agcgtcacga cggtccggac
     1141 cgacgcgtcg cgtgaggagc tcgatcggcg cttcgagctc gaggcgatcc ccctgctgga
     1201 ggagatgtac ggtgcggccc tccggctcac gcggaacccc gccgacgccc aggacctgct
     1261 ccaggagacc tacctccgag cctttcgggg gttcgccggc ttcgaggagg gcacgaacct
     1321 caaggcgtgg ctgtaccgga tcctgacgaa cagctacatc aacctctacc ggaagcgaca
     1381 gcgagagccc cagaccgtct cggacgacga ggtcgaggag tggtacctct actcgaggct
     1441 gcgcgagtcg gacctcgagc cttcggccga ggccgaggtg atcgagcggc tgcccgacga
     1501 ggacgtccag gccgcgctcg cctcgctgcc ggacgtgtac aggatggcgg tgatcctcgc
     1561 ggacatcgag ggcttctcgt acaaggagat cgcggccatc ctggacgtcc cgatcgggac
     1621 ggtcatgtcg cgcctccatc ggggaagaag ggcgctcgag cgggcgttgt gggacatcgt
     1681 gcgggagcgt ggcgttgtca gggattgagt gcgaggagat cctccgccag gtcgaggtgt
     1741 acctcgacgg cgagctgccg gcggaggagg ccgcccgcgt cgagggccat ctggccggat
     1801 gcagcccctg cctcgagcgc caggagttcg tgtcgaacct ccggcgcatc ctccgggaca
     1861 agtgcgggcg cgtcgagcgc ctgccggacg ccgcccgcga gcggatccac cgggcggtgg
     1921 cgcgggaggc cacgtccgag acggactgaa ccgggcccgt ctcggcggcg tatcaccctg
     1981 caaggagcgg tccggacgtc ccgggccgat cgcgtcatgc agggaaagcg accgggaagg
     2041 atgccccgcc catccaagct ggaggctgca ggcagggtct gcgcggcccc cgggtgcgag
     2101 acggggctct cgatctacaa cgccaagagc acctgctggc agcacaccga tctgacgttc
     2161 ccgaacttcc gcgggaagcg gctcgctaag ggctccgcct gagagcggtg gcgggagtcc
     2221 cgcggggtcc cctatactcg ggcgagaaac ccgctctgcg ccccgacgga cgcatgtcca
     2281 cggaacggag gaacacgtga cgaacgggag cgcaaacgca gagtatctga agactgcgga
     2341 ggtcgccgag attctccacg tctcgccgaa gacggtgacg cgatgggcga aggacggcaa
     2401 gctgcctcac tcgaggacgc tcggcgggca tcgacggttc ccggccgagg cgatcaggcg
     2461 cctggccgag ggcctggtgc aggtcgacga gatcgagacg ccgtagcggt cgcgagcgga
     2521 ccccggccgg gggacggatg cagatcctcc tcggcctcgt gatcctccag ttcggtctgg
     2581 gcctcgtgct ggccggcgtg gccgggcggg tgcgtcagga tgcggggggc tccttctggg
     2641 cgggcgtcgc cctctcggcc ctgctcggcc tggtggggtt ctggatcgtg gtggcctccg
     2701 cccggcgagc ctcccgatcg tgagcggcgc tcgtcgtctc gcgctcctcg ggtcggggga
     2761 gttcgagccg tgggccgagg aggtcgaccg ctggctgctg gccggtgcat ccgacggccg
     2821 cgtcctggtg gcgcccgccg ccagcgccgc ggaaggggag gaggtcttct cgcgttgggg
     2881 gcgcctcggc gtggagcact acgggcgcct cgggtacgcg gccgaggtgc tgcccctgag
     2941 gacgcgggac gacgcctcgg acccccggct cgcgtcgcgg ctcgacgagg cctcgatggt
     3001 gttcttctcg ggcggcaacc cggccgccct cgccgcgacg ctcgccggga cccccttctg
     3061 ggatcggctc ctccgccggc tgggggaggg gctcgcgttc ggcggctgca gcgcgggcgc
     3121 ggccttcctc ggggagctgg ccccggactc ctcgatcgcc gatccgctgg agggcgatcc
     3181 gtggaggccg ggggtgaagg cgttcggagg cgtcgtgctg gcgccgcact gggacaccgt
     3241 ggactcgcac gtccccgggt tccgggcggc ggtggagcgg atggcgggtc cgggcctcgt
     3301 cctcgtcggg gtggacgagc ggacggccct gctcggggac ggccggtcgt ggcgcgtgac
     3361 gggggagggg tcggtccacg tccgtctcca ggacgcatgg acctcgcacc cgcccggcgg
     3421 gacgctccct cccggcgtgc tcgaggtcgc tccggcttga cgagcgccgc tcgttctgcg
     3481 taaggttccg cccacgatga cgagtcccgt tcgcacctgg cgctgggcct ggcgcccctc
     3541 gggtggggcg ggaccgctca tggcctgatc acgccgtagc agccgcacca ctcgaggacc
     3601 tccgaccggg ggtccttttc tcgttcccgg agggagcttg gccgcgatca ccgacaccga
     3661 catcgatctc cacgccccgc tcggggccta cctgcggctg cgagagcggg gcccttcgtt
     3721 cctgctggag tcggtggagc ggggcaggct cggccgccac agcttcgtgg gtacgggctc
     3781 ccgcctcctg tccttcgagg aggccgaggc cgaggtcgct cgcggggcct cggtcgtcgg
     3841 ctacctgggc tacgatcacg cggcgcgcct cgagcccgtc ccgctcccgg tcgaagggcc
     3901 gggaccgccc gagagcctgt tcgtggcggc cgaggcgctc gtacgattcg atcacgtcct
     3961 ggggcgcgcc gaggtcctcg tcggggacgc ggcggagatc ggtcgcgccc tcgcccgccc
     4021 cgtcccgccc tcgccgcggt caccgggcct cccgggcccg atccggaggt cgccctcggc
     4081 cgacgcgtac cgggaggccg tgcgcgccgc caaggagcac atccgggcgg gcgacgtcct
     4141 ccagatcgtc ctctcccagc gagccgagcg ccccacgtcg gcgtccgcgg tcgccctgta
     4201 ccgggcgctg cgccggatca gcccctcccc ctacctcttc ctgttggagc tcggcgacct
     4261 ggcgctcgtg ggcgcctccc ccgagacgct ggtgaagctg gagggaagga aggccagcgc
     4321 gaacccgatc gccggcacga cggcgcgggg gctgggcgac gcgcagcgcc tcctcgcgtc
     4381 ggccaaggat cgggccgagc acgtgatgct cgtggacctg gcccggaacg acctctcgcg
     4441 cgtgtgccgg ccgggctcgg tcgtcctcga gcggttcctc gacgtcgaac cctactcaca
     4501 cgtcacccat ctcgtctcgg aggtcgtggg ggagctggag ccctccgcga gcggcttcga
     4561 tctcctgcgg gcgtgcttcc cggcgggcac cgtctccggc gccccgaagg tgcgggcgat
     4621 gcagctcatc tccgagctgg agggggtccg gcgcgggccg tacgggggag cggtggggta
     4681 cgcgctcccc gacgggagcc tggacgcgtg catcgcgatc cggatgatcg tcctggtgca
     4741 cggcgtcgcc cgcctccagg cgggcgcggg gatcgtggcc gcctcggatc ccgacctcga
     4801 gcaccgggag tgcctgcaga agctggccgc aatggagcag gcgatcgagg aggccgagcg
     4861 atgatcctgc tcctcgacaa ctacgactcg ttcacgcaca acctcgccca cctcctccgc
     4921 gtgctcggcg tggaggttct ggtggtgagg aacgacgcga tcacgcccga agaggcagag
     4981 gccgcggccc ccagccacct ggtgatctcg cccgggccgg ggtgcccggc cgaggcgggc
     5041 gcgacccggg cggtgctccg gagactcgcg gcccgcacgc ccaccctggg cgtgtgcctc
     5101 gggcatcaag cgatcgtgga ggtcttcggc ggggaggtgg ggccggcccg gaggctcgtc
     5161 cacggcgagg gggcctcgat ccgccacgac gggcgcgggg tgttccgagg cgtgccccag
     5221 ggcttcgtcg ccggccgcta tcactcgctc gccgctcgcc gcttgcccga cgcgctggag
     5281 gtcagcgccc gggcggagga cggtgaggtc atgggcgttc gacaccggtc cctgccggtg
     5341 gagggcgtgc agttccaccc cgagtcggtg ctgacgccgc tcgggccatc ccttgttcgc
     5401 aacttcctgg agcggaggtg aggcagatgt cgatcacgga cgcgctcgca cgcctgctgg
     5461 agggctcgga cctgtcccga gaggaggcgc gggccgtgac gggcgagatc atgcgggggg
     5521 aggccacccc cgcgcagatc ggggggttcc tggtgggcct gcggatgaag ggggagaccc
     5581 cggacgagat cgcggggtgc gcggaggcga tgcgcgacca cgtgctcccc gtatccccac
     5641 gccgctcgga ccttatcgac accgccggca ccggcggcga cggggccgga accctgaacc
     5701 tgtccacggc ggcggggctc gtagcggcgg cggccggggc gggtgtggcc aagcacggga
     5761 accgggccgt gtcctcggcc tcgggctcgg ccgacgtcct cgaggcgctg gggttctccc
     5821 tcgacctctc gcccgagcgg atcgccgagt cgatcgacga gcacgggttc gggttcctct
     5881 tcgcccctgc gcaccacccc gcgatgcggc acgcgggccc ggtccggcgg gagctggcgg
     5941 cgcgcacggt cttcaacgtg ctggggccgc tgacgaaccc ggccggggcc cgggcccagc
     6001 tcgtcggcgt gtacgcccgc gagttggccg ggaccgtggc gagggttctg gccctgctcg
     6061 gcgcggatcg agccctcgtc gtgcacgggg cgggcgggat cgacgagctg tcgccggcgg
     6121 gacccaacct cgtgttcgag gtcgaggaag gtcgtgtcga ggagaggatg gtggacccgg
     6181 ccgatctcgg gatcgacggg tgcgctcccg gcgacctccg gggcgggacg ccggaggaga
     6241 acgcggcggc catccgggag gtgttcgacg gcgcggccgg gccgaagcgg gacgcggtgc
     6301 tgctgaacgc ggcggccgcg ctacggatcg ccgggctcgt ctcggacctc cgggagggtc
     6361 tggccgcggc ggccgaggtc gtggacggcg gcgcggccgc cgcgaagctc gagacgctgg
     6421 tggcgttcag ccgcgccgag gaggctgtgg cgtgaggcgg ttccgacagg cgctcgcggc
     6481 tccgggcctc tccgccatcg ccgagatcaa gcggcgctct ccctcggccg gcgacctgcg
     6541 cccggacgcg gacgtctccc ggctggcccg cgccttcgag cgcacagggg cggcggcggt
     6601 gtccatcctc gtggacgagc gcttcgcggg gtccctgaag gacctccggg ccgcccgggc
     6661 cgccacggac ctgcccctgc tggccaaggg cttcttccgc accgaggagg acctggagga
     6721 ggtccgccgg gcgggcgccg acgcagcgct cctcctcctt cgcgacctgg acgatcgggc
     6781 ggcgcgacgg ctgatggccc gggccgaggc gatcgggctg gagaccctgg tggaggcgca
     6841 cgacggggac gagctggggc gggcgacccg gctcggggcc gacccggtcg ggatcaacgc
     6901 tcgggacctc tcgaccttcc gcgtggaccg ggcccg
//
LOCUS       DASUTW010000323         4524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_237551, whole genome shotgun sequence.
ACCESSION   DASUTW010000323 DASUTW010000000
VERSION     DASUTW010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237551"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1153)
                     /locus_tag="VF058_11165"
     CDS             complement(32..1153)
                     /locus_tag="VF058_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6208901.1"
                     /translation="MRRGTLARLGALVAVVALLAAACQRGEDAPEATDEEEPQAQQEG
                     GLLQEIRDRGMLRCGVNNTVPGFGFETSGGEIEGFDIDFCTAIAAAVFNTPDVEGMIE
                     LFPVDAEERFNALRAGEYDVLVRNTTWTSSRDGSEGVAFAHVNFYDGQAMMVREGEFS
                     AVDEMDGTSVCVTTGTTTELNLADYAAQVGVDIEPVTFAENPQLQEAFISGRCDGWTS
                     DRSQLAGIRSAWPEEEGGPESLLILDDVMSKEPLAPGVLDGDQDWHAAVDWVVNGLIL
                     AEELGVDSSNVEQQAQSPDNPQIAALLGVPFEAETETITVDSGLGLDNDFMQAVLEAV
                     GNYGEIFDRHLGPNTPLGLDRGVNALWTDGGIQYAMPFR"
     gene            complement(1275..2813)
                     /gene="purH"
                     /locus_tag="VF058_11170"
     CDS             complement(1275..2813)
                     /gene="purH"
                     /locus_tag="VF058_11170"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX6208902.1"
                     /translation="MSDLLPVRRALLSVADKEGLVVLGRGLVEEGVSIVSTGSTAQSL
                     RQEGVPVTSVAEVTGFPEMLGGRVKTLHPAIHAGILADKRDEEHLRQLEEAGIQPFDL
                     VVVNLYPFERTVRSGAARDEVVEQIDIGGPTLVRAAAKNFGSIGVIVDPERYREVLAA
                     IEGGGLPQRLRALLAEEAYRHVALYDAAIASWFGSWAGDGGLPRVLAPGFELVSPLRY
                     GENPHQRAGLYREALSDGPLGGAEVLQGKEMSYNNWLDAEAARALAGSFEEPACVIVK
                     HHNPCGTAATGDLADCYRDALASDPVSAFGGVVAFNREVDEATARAMQEVFTEVVVAP
                     GFSPKARQLLAGRENLRVVLAPPPAPGGLELRPIAGGALAQDADAVRELRDAMRVATR
                     RTPTEEEWEGLLFAWRVAARVKSNAIVLARGRATVGVGAGQMSRVDSVELAGRKAGDR
                     AQGSVLASDAFFPFRDGIDAAGEAGVTAVIQPGGSVRDEEVVAAADEHGMAMVLTGVR
                     HFRH"
     gene            complement(2810..3415)
                     /gene="purN"
                     /locus_tag="VF058_11175"
     CDS             complement(2810..3415)
                     /gene="purN"
                     /locus_tag="VF058_11175"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX6208903.1"
                     /translation="MNARLAVLASGSGTNLQALLDDPVVGPRVSLVVSDRGDAGALDR
                     AAAAGVKAVHLDPAIHDDRAAYDAALQDLLVSEGIDLVCLAGFMRILGQTLVRAFEGR
                     MLNVHPSLLPAFPGAHAPRDALAWGAKVTGVTVHVVDEEVDQGPIVLQEAVPVREDDD
                     AESLHARIKDVEHRVYPRAVRWWLEGRVRIDGRRVRIEGEA"
     gene            3439..3987
                     /locus_tag="VF058_11180"
     CDS             3439..3987
                     /locus_tag="VF058_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208904.1"
                     /translation="MSANACSVHPDVPARETCGRCGRAACLGCAVPVRGEIRCRECVG
                     DELGEAVEAAAPPRSVRFRDLPAGLLFLAAVLVSLLPWDRQGSRAGFLSAWTPVPEPW
                     PFVAGVLLLLAAVASLRGRRSRPRAVHIGLGVLAVLATLLALPSPGLSTRGPVPIVVL
                     GLAVAATLTGVVRATAAFGQRP"
     gene            4059..>4524
                     /locus_tag="VF058_11185"
     CDS             4059..>4524
                     /locus_tag="VF058_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208905.1"
                     /translation="MAQQGFDISRLTMGQKTLLITGLVFLVTLFFPWESFDVGGLPGF
                     ELPGVNISGWNGIGVLAGILAILLLVWELMLMAGVNLNLNVSEALISAGLAAATVLFG
                     LIRFVIALTRSGYGIAWGAWVGLIVLLAMAYGAWVRFNESKAMGTPPPAPPAA"
BASE COUNT          596 a         1711 c         1560 g          657 t
ORIGIN      
        1 gtcccgaacc cccgccccgc cgatcgtcgg tctacctgaa cggcatcgcg tactggatcc
       61 cgccgtcggt ccagagcgcg ttcacgcccc gatcgagccc gagcggggtg ttcgggccaa
      121 ggtggcggtc gaagatctcg ccgtagttcc ccacggcctc cagcacggcc tgcatgaagt
      181 cgttgtcgag cccgagcccg ctgtccacgg tgatcgtctc ggtctcggcc tcgaacggga
      241 ccccgagcag cgccgcgatc tgcgggttgt cggggctctg ggcctgctgc tccacgttcg
      301 agctgtcgac cccgagctcc tccgccagga tgagaccgtt caccacccag tcgacggccg
      361 catgccaatc ctggtccccg tcgaggacgc cgggcgccag cggctccttc gacatgacat
      421 cgtcgaggat gaggagcgac tcggggccgc cctcctcctc gggccacgcc gagcggatgc
      481 cggccagctg ggaccggtcg gacgtccagc cgtcgcagcg accggagatg aaggcctcct
      541 gcagctgcgg gttctcggcg aacgtcaccg gctcgatgtc cacgcccacc tgcgccgcgt
      601 agtcggccag gttgagctcg gtcgtcgtcc cggtcgtgac gcagacggag gttccgtcca
      661 tctcgtccac cgcgctgaac tcgccctcgc ggaccatcat cgcctggccg tcgtagaagt
      721 tgacgtgggc gaaggccacg ccctctgagc cgtcgcggct cgaggtccag gtcgtgttcc
      781 gcacgagcac gtcgtactct ccggcgcgga gcgcattgaa ccgctcctcg gcgtcgaccg
      841 ggaagagctc gatcatcccc tccacgtcgg gggtgttgaa caccgccgct gcgatcgcgg
      901 tgcagaagtc gatgtcgaac ccctcgatct caccgcccga ggtctcgaag ccgaacccag
      961 gcacggtgtt gttcacgccg caccggagca tcccccggtc ccggatctcc tggagcagac
     1021 ctccctcctg ctgggcctgc ggctcctcct cgtcggtggc ctcgggggcg tcctctcccc
     1081 tctgacaggc tgccgcgagg agcgcgacga cggctacgag cgcgccgagc ctcgccaagg
     1141 tgccccttcg catctctgcc tcccttcttc cggcgggccg agggccccga gcaggggccc
     1201 tgggcgttcg cgggcgagca tatcccgttc gacggaggcg ggcgagttgg gcggaagccg
     1261 cactccttgc tcccctaatg gcggaaatgc ctgaccccgg tgagcaccat ggccatgccg
     1321 tgctcgtccg ccgccgccac gacctcctcg tcgcggaccg atcccccggg ctggatcacg
     1381 gccgtcaccc ccgcctctcc ggccgcgtcg atgccgtccc ggaaggggaa gaatgcgtcg
     1441 gaggccagga ccgagccctg cgcgcggtcc ccggccttcc gccccgcgag ctccacgctg
     1501 tccacgcggc tcatctgccc ggcgcccacc cccacggtgg cccggccccg cgccaggacg
     1561 atcgcgttcg acttcacccg agcggccacg cgccaggcga agaggagccc ctcccactcc
     1621 tcttcggtcg gggtccgccg cgtcgcgacc cgcatcgcgt ccctgagctc gcggaccgcg
     1681 tcggcgtcct gggccagcgc cccgcccgcg atcggccgga gctccagccc gcccggcgcg
     1741 ggaggcggcg cgagaacgac gcgcaggttc tcgcgccccg caaggagctg gcgcgccttc
     1801 ggggagaagc cgggggccac caccacctcg gtgaagacct cctgcatcgc cctcgcggtg
     1861 gcctcgtcca cctcgcggtt gaaggccacg acgcccccga acgccgaaac gggatcggac
     1921 gcgagcgcgt cgcgatagca gtccgcgagg tcccccgtgg ccgccgtgcc gcagggattg
     1981 tgatgcttca cgatgacgca ggcgggctcc tcgaacgagc cggccagcgc ccgggccgcc
     2041 tccgcgtcca gccagttgtt gtaggacatc tccttgccct ggagcacctc ggctccgccc
     2101 agcggaccgt ccgagagcgc ctcccggtag aggccggccc gctgatgcgg gttctccccg
     2161 tagcggagcg gggacaccag ctcgaagccg ggggccagga cgcggggaag cccaccgtcc
     2221 ccggcccagg agccgaacca cgacgcgatc gcggcgtcgt agagggccac gtgacggtag
     2281 gcctcctccg cgagcaaggc gcgcagccgc tgggggagcc ccccgccctc gatcgccgcc
     2341 aggacctccc ggtagcgctc gggatccacg atcacgccga tcgagccgaa gttcttggcg
     2401 gcggcccgca ccagcgtcgg gccgccgatg tcgatctgct ccaccacttc gtccctggcc
     2461 gcccccgagc gcaccgtccg ctcgaacgga tagaggttca cgacgacgag gtcgaacggc
     2521 tggatgcccg cctcctcgag ctggcgcagg tgctcctcgt cccgcttgtc ggccaggatg
     2581 cccgcgtgga tcgccgggtg gagggtcttc acacgccccc cgagcatctc ggggaacccg
     2641 gtcacctccg caacggaggt caccggcacg ccctcctggc gcagggactg ggcggtggag
     2701 ccggtggaga cgatcgacac gccctcctcg acgaggcctc gccccagtac gaccagcccc
     2761 tccttgtcgg ccaccgagag gagcgcccgc cggacgggga ggaggtcgct catgcctcgc
     2821 cctcgatgcg cacgcggcgc ccgtcgatcc ggacgcgccc ctcgagccac cagcgcaccg
     2881 cccgcgggta gacgcggtgc tcgacgtcct tgatgcgcgc gtggagcgac tcggcgtcgt
     2941 cgtcctcgcg gacgggcacc gcctcctgga ggacgatggg cccctggtcc acctcctcgt
     3001 ccacgacgtg cacggtcacg ccggtgacct tcgcccccca cgccagcgcg tcgcgcgggg
     3061 cgtgggcccc ggggaaggcc gggagcagcg aggggtggac gttcagcatc cgcccttcga
     3121 aggcccggac gagggtctga ccgagtatgc gcatgaagcc ggccaggcac acgaggtcga
     3181 tgccctcgga gacgaggagg tcctggagcg cggcgtcgta ggcggcccgg tcgtcgtgga
     3241 tcgcgggatc gaggtgcacg gccttcacgc cggcggccgc ggcccggtcg agcgcgcccg
     3301 cgtcgccccg gtcgctcacg acgagggaca cccgcggacc cacgaccgga tcgtccagca
     3361 gagcctggag gttggtcccg gagcccgacg ccaggaccgc gagccgagcg ttcaccgggc
     3421 gcaaactacc atatgggcgt gagcgccaac gcctgctcgg tccacccgga cgtcccggcc
     3481 cgagagacct gcggacgatg cggacgggcc gcgtgcctgg gctgtgccgt ccccgtccgg
     3541 ggcgagatcc gctgcaggga gtgcgtgggc gatgagctgg gggaggcggt cgaggcggcc
     3601 gctccccctc gatccgtccg gttccgggac ctcccggccg gcctcctctt cctcgccgcc
     3661 gtcctcgtct ccctcctgcc ctgggaccgg caggggtcgc gggccgggtt cctctcggcg
     3721 tggacaccgg tccccgagcc gtggccgttc gtggcgggcg tgctgctcct cctggccgcg
     3781 gtggcgtcgc tccgcgggcg gcggagccgc ccccgagccg tccacatcgg gctgggggtg
     3841 ctggccgtcc tggcgacgct cctcgccctc ccctccccag ggctctcgac gcgcgggccg
     3901 gtgcccatcg tcgtcctcgg cctggcggtg gcggcgacgc tgacgggcgt cgtgcgggcc
     3961 accgcggcgt tcggccagcg tccttgactc gacccagcgg ggtcacgaga atcacgcgag
     4021 agaacccacc agcccggggg agaccgaaag gagaccgcat ggcgcagcag gggttcgaca
     4081 tctcgaggct cacgatgggt cagaagaccc tcctgatcac cggcctcgtc ttcctcgtca
     4141 cgctcttctt cccgtgggag agcttcgacg tgggcggcct gccggggttc gagctcccgg
     4201 gtgtgaacat ctccggctgg aacgggatcg gggtcctcgc cgggatcctc gccatcctgc
     4261 tcttggtctg ggagctcatg ctgatggccg gcgtcaacct gaacctgaac gtgtccgagg
     4321 cgctgatcag cgcggggctc gcggcggcca cggtcctgtt cgggctgatc cgcttcgtca
     4381 tcgcgctcac ccggagcggc tacgggatcg cctggggcgc gtgggtcggg ctgatcgtgc
     4441 tgctggccat ggcctacggg gcctgggtcc ggttcaacga gagcaaggcg atggggacgc
     4501 ccccgccggc tccccccgca gcgt
//
LOCUS       DASUTW010000324         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_498674, whole genome shotgun sequence.
ACCESSION   DASUTW010000324 DASUTW010000000
VERSION     DASUTW010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498674"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VF058_11190"
     CDS             complement(<1..358)
                     /locus_tag="VF058_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEX6208906.1"
                     /translation="MAGPLAGRRVLLAVTGGIAAYKAAYLCRLLQERGAEVQVVMTPS
                     ATRFVGPDTFAALTGRGVHQGVFERTETVLHVRLAREADAAVVAPATANVLAKLANGI
                     ADDLVTSTLLEFRGPLV"
     gene            complement(361..612)
                     /gene="rpoZ"
                     /locus_tag="VF058_11195"
     CDS             complement(361..612)
                     /gene="rpoZ"
                     /locus_tag="VF058_11195"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX6208907.1"
                     /translation="MIEPSIDDLLEKVDAKYALVILAARRAREINNYYSQLGEGRGEF
                     VPPLVETGGLREKPLTIAIQEIAEDKVTAERPEEPEAEE"
     gene            complement(609..1181)
                     /gene="gmk"
                     /locus_tag="VF058_11200"
     CDS             complement(609..1181)
                     /gene="gmk"
                     /locus_tag="VF058_11200"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX6208908.1"
                     /translation="MRGRLVVVSGPSGVGKGTVVRGLLAARPDLALSVSATTRDPRPG
                     EVDGVHYRFLGDAAFDRLIERGAFLEWAEVFGRRYGTLAEDVEEARAGGRDVLLEIDV
                     QGARTVRERVPDAILVFLQPPSEEELRRRLAARGTETGESLEARLREARREMAEADRF
                     DHVVVNDEISRAVVEVLAIIDAPIREGDPS"
     gene            complement(1200..1517)
                     /gene="mihF"
                     /locus_tag="VF058_11205"
     CDS             complement(1200..1517)
                     /gene="mihF"
                     /locus_tag="VF058_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HEX6208909.1"
                     /translation="MPLPSLTPEQRRQALEKAAEARKKRAAVKQELKKGSLSLRDVLS
                     RAGNDDILGKMRVSAVLESMPGVGKVRARKIMERLDISSSRRVRGLGSKQRKALLEEF
                     SRR"
     gene            complement(1614..2162)
                     /gene="pyrE"
                     /locus_tag="VF058_11210"
     CDS             complement(1614..2162)
                     /gene="pyrE"
                     /locus_tag="VF058_11210"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX6208910.1"
                     /translation="MIRELLEERGALLSGHFLLTSGKHSDVYVEKARMLEDPALVARL
                     AGEIASWYERVEAVVSPAVGAIPLGFAVAAAAGARFLYAERDRRQMTLRRGFALRPGE
                     RTLVVEDVVTTGGSAGEVWELVRSAGAEALGVAALVDRTAGPMAFPLRALVRVEARVF
                     EPGVCPLCSAGEPLAGRGSRHL"
     gene            complement(2159..>2411)
                     /locus_tag="VF058_11215"
     CDS             complement(2159..>2411)
                     /locus_tag="VF058_11215"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006559656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HEX6208911.1"
                     /translation="AGARGVVCSGDRVREVRDALGDAPLIVVPGIRPGGMGANDHARV
                     LTPREAVECGADLLVVGRPITQAADPVQAARAIARELEG"
BASE COUNT          330 a          917 c          851 g          313 t
ORIGIN      
        1 cgacgagcgg gccgcggaac tccaggaggg tcgaggtcac gaggtcgtcg gcgatgccgt
       61 tcgccagctt ggccaggacg ttggccgtgg cgggggccac gacagcggcg tcggcctcgc
      121 gagccagccg gacgtggagg acggtctcgg ttcgctcgaa gacgccctgg tggacgcccc
      181 ggccggtgag cgcggcgaag gtgtcggggc cgacgaagcg cgtagccgag ggggtcatca
      241 cgacctggac ctcggccccc cgctcctgca ggagcctgca gagataggcg gccttgtacg
      301 cggcgatgcc ccccgtgacc gccaggagga cgcgccgccc ggccaggggg ccggccaccc
      361 ctactcctcg gcctcgggct cctccggccg ctcggcggtg accttgtcct ccgcgatctc
      421 ctggatcgcg atcgtcagcg gcttctcccg gagcccgccc gtctcgacga ggggcggcac
      481 gaactcgccg cgcccctccc cgagctggct gtagtagttg ttgatctcgc gggcccggcg
      541 cgcggccagg atcacgagcg cgtacttggc gtcgaccttc tcgagcaggt cgtcgatgct
      601 gggctcgatc aagaggggtc tccttcgcgg atcggtgcgt cgatgatagc aagaacctcc
      661 accacggccc gggagatctc gtcgttcacg accacgtggt cgaaccggtc ggcctcggcc
      721 atctcccgtc gtgcttcgcg gaggcgcgcc tccagcgact cccccgtctc ggtgccccgc
      781 gcggccaggc gccgtcgaag ctcctcctcc gaggggggct ggaggaacac gaggatcgca
      841 tccggcacgc gctcccgcac ggttcgcgcg ccctggacgt cgatctcgag gagcacatcc
      901 ctgcccccgg cccgagcttc ctccacgtcc tcggcgagag ttccgtagcg gcggccgaac
      961 acctccgccc actccaggaa ggcgccccgc tcgatcagcc ggtcgaacgc tgcgtcgccc
     1021 aggaaccggt agtggacgcc gtccacctcg ccggggcgag ggtctcgcgt cgttgccgag
     1081 acggagagcg cgaggtcggg ccgggccgcg agcagcccgc gaaccacggt ccccttcccc
     1141 acacccgagg gcccggagac gacgacgagg cgacctcgca ccggcgccgc cccggcgcct
     1201 cagcgccgcg agaactcctc gaggagggcc ttccgctgct tcgaccccag cccgcgcacg
     1261 cggcggctcg aggagatgtc cagccgctcc atgatcttgc gggcccggac cttccccacg
     1321 ccgggcatcg actcgaggac ggccgagacc cgcatcttcc ccaggatgtc gtcgttccct
     1381 gcccggctca ggacgtcgcg gagcgagagg gagcccttct tcagctcctg cttgaccgcg
     1441 gctcgcttct tgcgggcctc cgcggctttc tcgagggcct gacgtcgctg ttcgggggtg
     1501 agcgatggca ggggcataca tcctccttca ggtggcttcg gaagcgccgg gattataccc
     1561 acgccctttt ccgggcggca atgcggaacg agcaggtcac aagcggcgga cgttcagagg
     1621 tggcgcgagc cgcggccggc gaggggctcc ccagccgagc agagcgggca caccccgggc
     1681 tcgaacaccc gggcttccac gcggaccagg gcccgcaggg ggaaggccat cggccctgcc
     1741 gtccggtcca cgagcgcggc gacgccgagg gcctcggccc cggccgagcg cacgagctcc
     1801 cacacctcgc cggccgagcc gccggtggtc acgacgtcct ccacgacgag ggtccgctca
     1861 ccgggccgca gggcgaagcc gcggcgcagc gtcatctgcc tcctgtcccg ctccgcgtag
     1921 aggaagcgcg cgccggcggc cgcggccacc gcgaacccga gggggatcgc gccgaccgct
     1981 ggggacacca cggcctcgac ccgctcgtac caggacgcga tctcgccggc gaggcgagcc
     2041 acgagcgcgg ggtcctcgag catccgggcc ttctccacgt agacgtcgga gtgcttcccc
     2101 gaggtgagca ggaagtgccc ggagagcagg gcgccccgct cctccagcag ctcccggatc
     2161 atccctcgag ctcccgggcg atggcccgcg cggcctggac ggggtcggcg gcctgcgtga
     2221 tcgggcggcc cacgacgaga aggtcggcgc cgcactccac ggcctcccgg ggggtcagga
     2281 cccgggcgtg gtcgttcgcc cccatcccgc ccgggcggat cccggggacg acgatcagcg
     2341 gcgcgtcgcc cagcgcgtcc cggacctccc ggacgcgatc cccggagcag accaccccgc
     2401 gggcgccggc c
//
LOCUS       DASUTW010000325         2466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_499355, whole genome shotgun sequence.
ACCESSION   DASUTW010000325 DASUTW010000000
VERSION     DASUTW010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499355"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..340
                     /gene="rpsS"
                     /locus_tag="VF058_11220"
     CDS             56..340
                     /gene="rpsS"
                     /locus_tag="VF058_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX6208912.1"
                     /translation="MPRSLKKGPFVDDHLLAKVEAMNQRGEKRVIRTWSRRSTIIPEM
                     VGHTIAVHDGRKHVPVFITEGMVGHKLGEFAPTRTFRTHGHHTERSTSLK"
     gene            347..706
                     /gene="rplV"
                     /locus_tag="VF058_11225"
     CDS             347..706
                     /gene="rplV"
                     /locus_tag="VF058_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX6208913.1"
                     /translation="MEARAISRYVRVAPRKARRVTDLIRGKHVDEARRILRFTPYGVN
                     QTVAKTLESAVANAGQLPGVVPENLFVTKAYVDEGPTLRRFRPRAMGRATRIDKRTSH
                     ITVIVGTTEEVPGGPED"
     gene            690..1526
                     /gene="rpsC"
                     /locus_tag="VF058_11230"
     CDS             690..1526
                     /gene="rpsC"
                     /locus_tag="VF058_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX6208914.1"
                     /translation="MGQKINPYGFRLGVIYPWRSKWYAGKREYAQALHEDIKIRDYIK
                     LHLSRAGISEIIIDRKGGDQIEVNIRTARPGIVIGRKGSEVERVRKDLERITAKRVSV
                     NVEDLNVAATDSRPETDATLLAQGVAEQLKGRVSFRRAMRRAVQTAMRAGALGVRVQC
                     SGRLGGMEMSRREWYREGRVPLHTLRAKIDYGLAEARTTFGMIGVQVWVYHGDEIPQE
                     EQETERARARALAGIGGASTGALVTDEKEMAEVAEEPAAAKPAEGDEATSSEGRSEGG
                     SA"
     gene            1526..1945
                     /gene="rplP"
                     /locus_tag="VF058_11235"
     CDS             1526..1945
                     /gene="rplP"
                     /locus_tag="VF058_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX6208915.1"
                     /translation="MLQPKKVKWRKQQRGRMRGKAKGGTDVQFGDYGLAALEPGWISN
                     RQIEAARVAITRYIRRGGKVWINIFPDKPVTKKPAETRMGSGKGNPEDWVAVVKPGRV
                     MFELSGVSETVAREALRRGAAKLSVRSKFVTRFGGGA"
     gene            1942..2181
                     /gene="rpmC"
                     /locus_tag="VF058_11240"
     CDS             1942..2181
                     /gene="rpmC"
                     /locus_tag="VF058_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013805286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX6208916.1"
                     /translation="MTKPAELRDLDRNELLERLEAAKEELFNLRFQFATGQLDNPMTI
                     KAVRHDMARILTILRERELEELEAEEAAGEEEEAR"
     gene            2178..2438
                     /gene="rpsQ"
                     /locus_tag="VF058_11245"
     CDS             2178..2438
                     /gene="rpsQ"
                     /locus_tag="VF058_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005253856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX6208917.1"
                     /translation="MSAERGSRKVRTGVVVSNAMDKTVVVRVDGQLRHPLYRKIVRRT
                     SKLRAHDEGNEVGVGDRVRIVETRPLSKTKRWRVVEIVEKAK"
BASE COUNT          446 a          802 c          921 g          297 t
ORIGIN      
        1 gaaggcctcc gaccgctaca tcatccgccg ccgcggcaag gggaggggct gaggcatgcc
       61 gaggtcgctg aagaagggac cgttcgtgga cgaccacctc ctggccaagg tggaggcgat
      121 gaaccagcgc ggcgagaagc gggtgatccg cacgtggtcg cgccgctcga cgatcatccc
      181 cgagatggtc gggcacacga tcgccgtcca cgacggcagg aagcacgtcc cggtgttcat
      241 caccgagggc atggtgggcc acaagctggg cgagttcgcg cccacgcgga ccttccgcac
      301 ccacggccac cacaccgagc gaagcacgtc cctgaagtag gagccgatgg aagcgagagc
      361 gatcagcagg tacgtgaggg tcgcgccgag gaaggcgcgc cgggtcaccg acctcatccg
      421 cggcaagcac gtggacgagg cgcggcggat cctgcgcttc accccctacg gcgtcaacca
      481 gaccgtggcc aagaccctcg agtcggccgt ggccaacgcc gggcagctgc cgggcgtcgt
      541 acccgagaac ctgttcgtca cgaaggccta cgtggacgag gggcccaccc tgcgccggtt
      601 ccgtccgcgc gccatggggc gggccacgag gatcgacaag cgaaccagcc acatcaccgt
      661 gatcgtgggg acgaccgagg aggtgcccgg tgggccagaa gattaatccc tacggcttcc
      721 gcctcggcgt gatctacccg tggcgctcga agtggtacgc gggcaagagg gagtacgcgc
      781 aggcgctcca cgaggacatc aagatccgcg actacatcaa gctgcacctg tcgcgggccg
      841 ggatctccga gatcatcatc gaccgcaagg gcggcgacca gatcgaggtg aacatccgga
      901 cggcgcggcc ggggatcgtg atcggccgga agggctccga ggtcgagcgg gtgcgcaagg
      961 atctcgagcg gatcaccgcc aagcgcgtgt ccgtcaacgt cgaggacctc aacgtggcgg
     1021 ccaccgactc ccgccccgag accgacgcca ccctgctggc gcagggcgtg gccgagcagc
     1081 tcaagggccg cgtctcgttc cgccgggcca tgcgccgggc ggtgcagacc gcgatgcggg
     1141 ccggggctct gggcgtccgg gtgcagtgca gcggccgcct gggcggcatg gagatgtcgc
     1201 gccgcgagtg gtaccgcgag gggcgcgtcc ccctccacac cctccgggcc aagatcgact
     1261 acgggctggc cgaggcccgc accaccttcg gcatgatcgg cgtccaggtc tgggtctacc
     1321 acggcgacga gatcccgcag gaggagcagg agacggagcg agcccgcgcc cgagcgctgg
     1381 ccggcatcgg cggggcctcg accggcgcgc tcgtcaccga cgagaaggag atggcggagg
     1441 tggccgagga gcccgcggcg gcgaagcccg ccgagggcga cgaggccacc tcgagcgagg
     1501 gccgctccga gggaggctcg gcctgatgct gcagccgaag aaggtcaagt ggcggaagca
     1561 gcagcgcggc cggatgcgtg gcaaggccaa gggcggcacc gatgtgcagt tcggcgacta
     1621 cggccttgcc gccctggagc cgggctggat cagcaaccgc cagatcgagg cggcccgcgt
     1681 ggccattacc cggtacatcc gccgcggcgg gaaggtgtgg atcaacatct ttcccgacaa
     1741 gccagtgacg aagaagccgg ccgagacgcg catgggctcg gggaagggca accccgagga
     1801 ctgggtggcg gtggtcaagc ccggccgggt gatgttcgag ctctccggcg tcagcgagac
     1861 cgtggcccgc gaggccctgc gccgcggcgc cgcgaagctc tccgtccgca gcaagttcgt
     1921 gaccaggttc gggggtgggg cgtgacgaag cccgccgagc tccgtgacct ggaccggaac
     1981 gagctgctgg aacggctgga ggcggcgaag gaggagctgt tcaacctccg cttccagttc
     2041 gccaccggtc agctcgacaa cccgatgacg atcaaggcgg ttcgtcacga catggcccgg
     2101 atcctgacga tccttcgcga gcgggagctc gaggagctgg aggccgagga ggccgcgggc
     2161 gaggaggagg aggcccgatg agcgccgagc gcggcagcag gaaggtgcgg acgggcgtcg
     2221 tcgtgtcgaa cgcgatggac aagaccgtcg tggtgcgcgt cgacggacag ctccgccacc
     2281 ccctgtaccg gaagatcgtc cggcggacgt cgaagctccg ggcccacgac gagggcaacg
     2341 aggtcggcgt gggcgaccgc gtgcgcatcg tggagacccg tcccctgtcc aagacgaagc
     2401 gctggcgcgt ggtcgagatc gtggagaagg ccaagtgatc cagcaggagt ccagggtcaa
     2461 ggtcgc
//
LOCUS       DASUTW010000326         7211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_760146, whole genome shotgun sequence.
ACCESSION   DASUTW010000326 DASUTW010000000
VERSION     DASUTW010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7211
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760146"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..531)
                     /locus_tag="VF058_11250"
     CDS             complement(1..531)
                     /locus_tag="VF058_11250"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEX6208918.1"
                     /translation="MRIIAGTAKGTRLAAVPKGTRPLSDRAREGLFSSLGERIVGARV
                     LELFAGTGAMGLEALSRGAASATFVDSASDAVRTIRENLIRTGLQGEVVRSDALRFLS
                     GPGEFDLVLLDPPWRHPEADVRDVLETLEPHLASGATVAITRPRRASTDVIPVNLQVV
                     KHLDYGETRILVLEAR"
     gene            complement(528..1124)
                     /locus_tag="VF058_11255"
     CDS             complement(528..1124)
                     /locus_tag="VF058_11255"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6208919.1"
                     /translation="MRRPLFSRGYEVFSRAEDRIISGYREETAGGARGRVLEIGAGNG
                     LNFARYRDARVIALEPVADMLPRARARAVEAPVPVHLVRGVAEALPFDEEAFDTVVVS
                     LVMCSVPDPAGVAAEIRRVLKPGGQVRFFEHVRAHDPRLARRQDRIGPLYTRFTGGCR
                     MNRDTLTVLRLAGFRVRYRRLSYGPKHLPHVIGVATPP"
     gene            complement(1124..3271)
                     /gene="recG"
                     /locus_tag="VF058_11260"
     CDS             complement(1124..3271)
                     /gene="recG"
                     /locus_tag="VF058_11260"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX6208920.1"
                     /translation="MKISVRTSISSFAGWAKRSVPGLRKLGIATVGDLLHHYPRRYID
                     RSNVASIGDLHVNDDATFIGTVRRVRRAFARNRRQMVTVTVFDPSGSIDLFWFNQSWP
                     AQRFREGMEVAVSGKVGAFRGRLRVTNPAVEILKGGGDETVHVGRIVPVHPAGEGVST
                     GVIRRLVHDALERVDEVPDPMPADVLARHGLPPLAEALQGVHFPETDAAKDRAIERLK
                     LDELFFLELGVAFRKRRVEREAEGIPHDLSGDLAERFVGALPFEITDAQRRTMDEVAK
                     DMAEPRPMHRLLQGDVGSGKTVVALYAALIAIQSGRQAAVMAPTEVLAQQHYRNIRAL
                     LAGIGARSVTEGPLDPQIDLFDPDPGEHNLTYALLTGAVTGAERREVLEGLADGSVQL
                     VVGTHALVQEGVGIRDLSLAVVDEQHRFGLHQRIALKEKGAQPDVLIMTATPIPRTLA
                     LTYYGDLDVSVLDEMPPGRRPVKTRVVRTPQQRRKAYELVRSEVEKGRQAFVVCAAID
                     EGNRLEVKAAEREAERLQTEVFPDLRITLLHGRMRPREKDERMEAFRSGSSDLLIATT
                     VIEVGVDVPNATVMLIENAERFGLAQLHQLRGRIGRAEHESFCILFDESEPDNEDAKA
                     RLQAMARTTDGFELADEDLRLRGEGTLFDIRQSGMPDLKLAQLAQDTDLVRVARRDAF
                     GLIEADPDLSEHPEQRAELERRFAERIDWLFHS"
     gene            complement(3268..4863)
                     /locus_tag="VF058_11265"
     CDS             complement(3268..4863)
                     /locus_tag="VF058_11265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEX6208921.1"
                     /translation="MGATLDAPTLRRAMTLYARALEAHREEIDSLNVFPVPDGDTGTN
                     MLLTQQAVVEALEKTSDASLAETGETIARASLMGARGNSGVILSQILRGLCARLGEGE
                     ADGRALAEALARADALARSAVAEPVEGTILSVLHDAAERAGDGDVGAVAEGAFGAAEA
                     SLVRTRDQLAALRDAGVVDAGGKGLVLLLDALRAAIRGGDLRTRAGPPGPVGHRDTPG
                     TTGSRYGFETMFLLETADGELGSLREALRRIGDSVVVVGGGGLYNVHVHTDDPDAAVE
                     AGRAVGEPREVRVTSLDERVARHCLAGQARGAREAEEEQVGRLPLVAVVEGAGLEGIF
                     RSLGATVVRGGPGNNPAVGDLVRALERTPGDLVFLLPNHPNVWPAAKAAAREAGRAEV
                     VRTRSASQGIAAALAYSTGDGSAPMSDAARRTASGRLARAERAAETPGGGVAPGDWVG
                     IDDADGDIWAAGGDAAAVAARLVTDRLATDRHELATVYWGSEGTREGAEAVAWAIRGA
                     LADLETDVQRGDQPRYPYLIALE"
     gene            4920..5426
                     /locus_tag="VF058_11270"
     CDS             4920..5426
                     /locus_tag="VF058_11270"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX6208922.1"
                     /translation="MIARPAVVALVTLLAVACGGTDVGETPRAGPTLHEDRLALPEYD
                     PDRFRALLRTLEGTPVVVNVWASWCGPCRVEGPHLASLAREYEGRVQFLGVDILDNRE
                     AARDFILELDWPYPSVFDPQGEIRDSLGLLGQPVTLVVDEAGEVVFEWSGAVAEDQLR
                     AEIEAVIS"
     gene            5454..7145
                     /locus_tag="VF058_11275"
     CDS             5454..7145
                     /locus_tag="VF058_11275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEX6208923.1"
                     /translation="MARPRLSSAAILVCVLLAQAPPAAAQQQPPRRVRVAVPDGSSVL
                     VHGDYPPIQSSCVDPVQPVLHERYRGAVEVLRADDGSLTVLGELGFEDYLKGIAEVPR
                     SWPEEALKAQVVAARSYAASRLDPGGEYDLCATDACQVYMGMGVESGAFGRRWAEAVE
                     ATAGRILLFQGRPAQTFYSSTSNGRTYTNAEVWGGDPLPYLPSQPERDDGESPVSRWR
                     VEVPFADLERLLRADGRWSGGGLRRVRVEQGAVLLRGRRLGARLSKAELRDALNDTAR
                     CLLPDRYPPTEPDGTRLPMTVPSTWFRMRTEGRALVLEGRGWGHGVGMVQWGAKGKAD
                     RGLSYEEILAHYYGGLRPQSYDLPGSIRVLLATGLTSVTVEPAGEARIAIRGVGSPPS
                     PPLRVTPTPRGIRVTTSAPPAPRLEIMRFGIRPRAGGGIRATLVTESAVKVRAEFLQD
                     DGIVRSRWRSLEAGQVRFRLPAPVPAGPTQVRLRVTDGVDAIVTGTRAVLVEGSATVG
                     PSPSPSATGAEVSAPRPEPDDQGPSVLLMLGLAAGAALVLGLVLTRSRRRGLHRR"
BASE COUNT          954 a         2706 c         2563 g          988 t
ORIGIN      
        1 tcaccgagcc tccaggacga ggatgcgcgt ctccccgtag tcgaggtgct tgacgacctg
       61 caagtttaca ggaatcacat cggtggaagc gcggcgggga cgggtgatcg ctaccgtggc
      121 tcctgaggcc agatgaggct ctagggtttc cagaacgtcc cgaacgtccg cttcgggatg
      181 ccgccagggc gggtccagca ggacgagatc gaactccccg gggcccgaga ggaagcggag
      241 agcgtccgag cggacgacct ctccctggag gccggtgcgg atcaggttct cccggatcgt
      301 gcggaccgca tcggaggccg agtccacgaa cgtggccgag gcggcgcccc gcgacagcgc
      361 ctcgagcccc atggcccccg ttcccgcgaa cagctcgagc acgcgggccc caacgatccg
      421 ctcccccagg ctggagaaca gtccctctcg ggcccggtcg gagaggggcc gcgtgccctt
      481 ggggacggcg gcgaggcgcg tacccttcgc cgtgcctgcg atgatcctca cggcggcgtg
      541 gcgaccccga tcacgtgtgg caggtgcttc gggccgtagg acaagcgacg gtagcggacc
      601 cggaaacccg ctaggcgcag gacggtgagc gtgtctcggt tcatccggca cccgcccgtg
      661 aacctcgtgt agagggggcc gatccggtcc tgacgcctcg ccagccgagg gtcgtgggcc
      721 cggacgtgct cgaagaaccg gacctgaccg ccgggcttga ggacgcgccg gatctcggcc
      781 gccacgcccg ccgggtcggg cacgctgcac atcacgaggc tcaccacgac cgtgtcgaag
      841 gcctcttcgt cgaacggcag cgcctcggcc acgccgcgca cgaggtgaac ggggaccggc
      901 gcctcgacgg cccgagctcg cgccctcgga agcatgtccg ccactggctc gagggcgatc
      961 acccgggcgt cgcggtaccg cgcgaagttg aggccgttcc ccgccccgat ctcgaggaca
     1021 cgtccccgcg ccccgcccgc cgtctcctcc cgatagccgc tgatgatccg gtcctcggcc
     1081 cgggagaaca cctcgtaccc ccgagagaag agcggcctgc gcatcagctg tggaagagcc
     1141 aatcgatccg ctcggcgaac cggcgctcca gctcggcccg ctgctcgggg tgttcggaca
     1201 ggtcggggtc ggcctcgatc aggccgaagg cgtcgcggcg agccacgcgc accaggtcgg
     1261 tgtcctgcgc cagctgggcg agcttgaggt cgggcatccc agactgccgg atgtcgaaga
     1321 gggtcccctc gccccggagc cgcagatcct cgtcggccag ctcgaagccg tcggtggtcc
     1381 gggccatcgc ctggaggcgc gctttcgcgt cctcgttgtc gggttccgac tcgtcgaaga
     1441 ggatgcagaa cgactcgtgc tcggctcggc cgatgcgtcc ccggagctgg tggagctggg
     1501 ccagcccgaa ccgctcggcg ttctcgatca gcatcacggt cgcgttgggg acgtccacgc
     1561 ccacctcgat cacggtggtg gcgatgagga gatccgagga ccctgagcgg aacgcctcca
     1621 tccgctcgtc cttctcccgg ggacgcatcc gtccgtggag cagcgtgatg cggaggtcgg
     1681 ggaagacctc ggtctggagg cgctcggcct cgcgctcggc ggccttgacc tcgaggcggt
     1741 tcccctcgtc gatcgcggcg cagacgacga aggcctggcg ccccttctcg acctcggaac
     1801 gcacgagctc gtaggccttc cgccgctgct gcggcgtccg gaccacccgg gttttgacgg
     1861 ggcggcggcc gggcggcatc tcgtcgagca ccgacacgtc caggtcgccg tagtacgtga
     1921 gcgccagcgt ccgagggatc ggcgtggcgg tcatgatcag gacgtccggc tgggcgccct
     1981 tctccttcag cgcgatccgc tgatggaggc cgaagcggtg ctgctcatcc acgaccgcca
     2041 gcgagaggtc ccgtatcccg accccctcct ggacgagcgc gtgggtcccc acgacgagct
     2101 ggacgctgcc gtcggccaag ccctcgagga cctcccgccg ctcggcgccc gtgaccgcgc
     2161 cggtcaggag ggcgtaggtg aggttgtgct cccccggatc cgggtcgaag aggtcgatct
     2221 gggggtcgag cgggccctcg gtgaccgacc gcgccccgat cccggcgagc agggcccgga
     2281 tgttccggta gtgctgctgg gccaggacct cggtgggggc catcacggcg gcctggcggc
     2341 cagactggat cgcgatcagc gccgcataga gggcgacgac ggtcttcccc gagcccacgt
     2401 ccccctggag gaggcggtgc atcggccgag gctcggccat gtccttcgcc acctcgtcca
     2461 tcgtccgccg ctgggcgtcc gtgatctcga agggaagggc gccgacgaag cgctcggcca
     2521 gatccccgga gaggtcgtgg gggatgccct cggcctcgcg ctccacccgc cgcttgcgga
     2581 aggccacccc cagctccagg aagaagagct cgtccagctt cagccgctcg atcgcccggt
     2641 ccttcgccgc gtcggtctcc gggaagtgga cgccctggag cgcctcggcc agcgggggca
     2701 gcccgtggcg cgccagcacg tcggccggca tcggatcggg gacctcgtcc acccgctcca
     2761 gggcgtcgtg gaccaggcgt cggatcacgc cggtcgagac gccttcgccc gcggggtgaa
     2821 cgggcacgat ccggcccacg tggacggtct cgtcgccccc gcccttgagg atctccaccg
     2881 cggggttcgt gacccgaagc ctcccgcgga aggcccccac cttgcccgag accgcaacct
     2941 ccatcccctc gcggaaccgc tgggcgggcc aggactggtt gaaccagaag agatcgatcg
     3001 agccgctggg gtcgaagacg gtgacggtga ccatctgccg gcggttccgg gcgaaggcgc
     3061 ggcggacgcg gcggacggtc ccgatgaacg tggcgtcgtc gttcacgtgg aggtcgccga
     3121 tcgaggccac gttggagcgg tcgatgtagc ggcgcgggta gtggtggagg aggtcgccga
     3181 ccgtcgcgat cccgagcttg cggagcccgg gcacggatcg cttggcccat ccggcgaagg
     3241 acgagatcga ggtccggacg ctgatcttca ttccagcgcg atcaggtagg ggtagcgcgg
     3301 ctggtcgccc cgctgcacat cggtctcgag atccgcgaga gccccgcgga tcgcccaggc
     3361 cacggcctcc gcaccctccc gggtgccctc cgatccccag tagacggtgg ccagctcgtg
     3421 gcggtcggtg gccagccggt ctgtcacgag gcgtgccgcg acggcggcgg cgtcgccccc
     3481 ggccgcccag atgtctccgt cggcatcgtc gatccccacc cagtccccgg gggcgacgcc
     3541 gccgcccggg gtctcggccg cgcgctcggc ccgggccagc cggcccgagg ccgtccgccg
     3601 ggccgcgtcc gacatggggg ccgatccgtc ccccgtcgaa tacgcgagcg ccgcggcgat
     3661 gccctgggaa gccgagcggg tgcgcacgac ctccgcgcgg ccggcctccc tggccgcggc
     3721 ctttgcggcc ggccacacgt tggggtgatt ggggagcaga aagacgagat cccccggcgt
     3781 ccgctccagc gcccggacca ggtcgccgac ggccgggttg ttcccggggc caccccgcac
     3841 gaccgtcgcc cccagcgagc ggaagatgcc ctccagaccc gctccctcca ccacggccac
     3901 caggggcagc cgccccacct gctcctcttc ggcctcgcga gctccccgag cctggcccgc
     3961 gaggcagtgc cgggccaccc gctcgtcgag agacgtgacc cggacctcgc ggggctcgcc
     4021 gaccgcgcga cccgcctcga ccgcggcgtc ggggtcgtcc gtgtgcacgt ggacgttgta
     4081 cagaccaccg ccgcccacca cgacgaccga gtccccgatc cgccggaggg cctcgcgcag
     4141 ggatcccagc tccccgtccg ccgtctccaa caggaacatc gtctcgaacc cgtacctcga
     4201 gcccgtggtc cccggcgtgt cgcggtgacc gacggggccg ggcggtcccg cccgtgtccg
     4261 gaggtcgccc cctcggatcg ccgcgcggag ggcatccagg agcagcacca gccccttccc
     4321 gccggcatcg acgaccccag cgtcgcgcag ggccgcgagc tgatcacggg tgcgcaccag
     4381 cgaggcctcc gcggcgccga acgccccctc cgccacggcc ccgacgtccc cgtcgcccgc
     4441 ccgctcggcg gcgtcgtgga gcacggagag gatcgtcccc tccaccggct cggccaccgc
     4501 gctcctcgcc agggcgtcgg cccgcgccag cgcctcggcc agcgcccggc cgtcggcctc
     4561 cccttcgccg agccgggcgc agagaccccg gaggatctgc gagaggatca cgcccgagtt
     4621 cccccgggcc cccatcagcg acgcgcgggc gatcgtctcc cccgtctccg ccagcgatgc
     4681 atcgctcgtc ttctccagcg cctccacgac cgcctgctgg gtgaggagca tgttggtccc
     4741 ggtgtccccg tcgggcacgg ggaagacgtt cagcgaatcg atctcctccc ggtgggcctc
     4801 cagagcccgt gcgtagaggg tcatcgcccg ccggagcgtc ggcgcgtcga gggtcgctcc
     4861 cacctcgccc acatcctatc cctcgacccc tcccccgccg gccgttgtac cgtgagggca
     4921 tgatcgctcg gcccgcggtc gtcgccctcg tgaccctcct ggccgtggcc tgcgggggga
     4981 ccgacgtcgg agagacgccc cgagccgggc ccacgctgca cgaggaccgc ctcgcgctcc
     5041 ccgagtacga tcccgaccgc ttccgcgccc tcctccggac gctcgagggc acaccggtcg
     5101 tggtcaacgt ctgggcctcg tggtgcgggc cgtgccgggt ggagggcccg cacctcgcgt
     5161 cgctggcccg agagtacgag ggccgcgttc agttcctggg ggtggacatc ctcgacaacc
     5221 gcgaggctgc ccgcgacttc atcctcgagc tcgactggcc ctaccccagc gtcttcgacc
     5281 ctcaggggga gatccgggac agcctgggtc tgctgggcca gcccgtcacg ctggtggtgg
     5341 acgaggccgg cgaggtcgtc ttcgagtgga gcggcgcggt ggccgaggac cagctcaggg
     5401 ccgagatcga ggccgtgatc tcctagcggg aggccggtgc taccgtggat cgcatggccc
     5461 gcccacgcct gtcgtcggcc gcgatcctgg tctgcgtcct gctggcccag gctccgccgg
     5521 cggccgccca gcagcagcca ccgcgccgcg tcagggtggc cgtgccggac ggctcctcgg
     5581 tcctcgtcca cggcgactac ccgccgatcc agtcctcgtg cgtggatccc gtccagcccg
     5641 tcctccacga gcgctaccgc ggagcggtgg aggtgctccg ggccgacgac ggctcgctca
     5701 ccgtgctcgg cgagctcggg ttcgaggact acctgaaggg catcgccgag gtcccccgat
     5761 cctggcccga ggaggcgctg aaggcacagg tcgtggcggc ccgctcctac gccgccagcc
     5821 gcctcgaccc cggcggcgag tacgacctct gcgccaccga tgcctgccag gtctacatgg
     5881 ggatgggggt cgagagcgga gcgttcggaa ggcggtgggc ggaggccgtc gaggccacag
     5941 cgggccggat cctcctgttc caggggagac ccgcgcagac cttctactcc tcgacctcga
     6001 acggcaggac ctacacgaac gcggaggtct ggggcggcga ccccctgccc tacctcccct
     6061 cccagcccga gcgggacgac ggcgagtcgc cggtctcccg ctggcgcgtg gaggttccct
     6121 tcgccgacct cgagcgcctc ctcagggcgg acgggcggtg gagcggcggc ggcctccggc
     6181 gcgtccgggt cgagcagggc gcggtcctcc tccggggccg gcggctcggc gcccggctga
     6241 gcaaggccga gctccgcgac gcgctgaacg acacggcccg atgtctcctc cccgaccgct
     6301 acccgcccac ggagcccgac gggacgaggc tccccatgac ggtcccctcg acgtggttcc
     6361 gcatgcggac cgagggccgg gccctggtgc tcgagggccg cggatggggg cacggcgtgg
     6421 ggatggtcca gtggggggcg aaggggaagg ccgaccgggg cctctcctac gaggagatcc
     6481 tcgcccacta ctacgggggc ctgcggccgc agagctacga cctcccgggc tcgatccgcg
     6541 tgctcctcgc gaccgggctg acctcggtga cggtggagcc cgcgggggag gcgcggatcg
     6601 cgatccgagg ggtgggcagc ccgccctccc ccccgctcag ggtcacgccg acgccccggg
     6661 ggatccgggt gacgacctcc gcgcctcccg ccccgcggct ggagatcatg cgcttcggga
     6721 tccggcctcg ggccggcggc gggatccgcg cgacgctcgt caccgagtcg gcggtcaagg
     6781 tccgggccga gttcctccag gacgacggga tcgtgcgcag ccgctggcgg tcactcgagg
     6841 ccgggcaggt ccggttccgg ctgcccgctc cggtgccggc cggccccacg caggtgcgcc
     6901 tccgggtcac cgacggcgtc gacgcgatcg tgaccggcac gcgggcggtg ctcgtggagg
     6961 gctccgccac cgtgggtccg agcccgtccc cgagcgcgac cggggccgag gtgtcggccc
     7021 ccaggcccga gcccgacgac caaggcccgt ccgtcctctt gatgctcggc ctcgccgccg
     7081 gggcggccct cgtcctgggc ctcgtgttga cccggagccg ccgcaggggg ttacatcgaa
     7141 gatgatgccg ctcgtcgccg agcacgtgcc gatcatctcc ctcgactggc tgaccagcca
     7201 cgggctgatc a
//
LOCUS       DASUTW010000327         6706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_761538, whole genome shotgun sequence.
ACCESSION   DASUTW010000327 DASUTW010000000
VERSION     DASUTW010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761538"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="VF058_11280"
     CDS             complement(<1..1128)
                     /locus_tag="VF058_11280"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 domain-containing protein"
                     /protein_id="HEX6208924.1"
                     /translation="MDTGNRTTDRSGHALLTAALILLAGVTGAAFGRVFEGGRPAARL
                     VLAAAAAVALAALLERRHLALSVATAAAAALVTIGLLVFPATTWFGLPTPSTLDALGR
                     ALGNLGRRAVEEAAPAPAIPALFAPSIIAVWAAAYGAHALAARAASPILALVPPAGLI
                     GFANVVAEDGARPAYAAAFLVAALAVVYTSGMRQLDLWGPVLPRRSLSAARLAAGPSG
                     RLARRLGLAATAVAVLLPGLLPGFGADAVLDLDGGSGGRVAISPLVDIRPNLMRDEPL
                     ELFTIEADRPAYWRLQALDEYTGRFWRPGDPRGDPAAVPGGQGLLPQPPGEGPALTQH
                     VRVTRLGGRYLPAAHTPVEVAAVGLDLRHDAERGSLIVPGSL"
     gene            complement(1080..2273)
                     /locus_tag="VF058_11285"
     CDS             complement(1080..2273)
                     /locus_tag="VF058_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX6208925.1"
                     /translation="MLTRRGLSVLAAAALLWVAARMTGSPDLHMVALGLLLLPALAAL
                     FIRWSRHGLRATRRLGQRRVFAGSPLRVAIEVQNTSRSAASLVLLEDRLPPSLGRAAR
                     AVLGGLRGRHRQTISYTVHCGRRGRFHIGPLIASVTDPFGLAQRKQEFPDVHELIVYP
                     HVDDLRAPSPARTAGGAGDTTARQLFRSGEEFFTMREYEIGDDLRRIHWPSTARTGRL
                     MIRQDEAGRRASATVFLDSRRAVYGADQDLERAVSAAASVGTLYLRGGIDLRLGTVDL
                     APQRMAPEAFLEALALTDPSPERQLGRPLVPLRDRSATGSALLFVTGLLADEDLSALI
                     AATGAYGQKVAVLVVAGDGDEVRSARVRLSRAEWRTVLLTPDRRLQEAWTLGTARPIA
                     AATRS"
     gene            complement(2276..3235)
                     /locus_tag="VF058_11290"
     CDS             complement(2276..3235)
                     /locus_tag="VF058_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018350911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX6208926.1"
                     /translation="MAASSDWERFRATFDAIRANIDHVIQGKSRETRLALAALVAEGH
                     VLIEDVPGVGKTMLAKAIARSIDCSFRRIQFTPDLLPTDITGVNVFNQEQQDFEFKPG
                     AIFANIVLGDEINRASPKTQSALLESMEEHQVTVDGATYALGVPFMVIATQNPIEHEG
                     TYALPEAQLDRFMIRLSIGYPEAEAEAEILTAHGSHSALEDIDPVADAGGVAELIAMA
                     REVHVAEPIRRYIVDLVGATRSHPDLYLGASPRASLMLMRAARAYAASEGRDYVLPDD
                     VKALLVPVLSHRLIVAPDAAMSGHSASSLLVGLLDEVPVPVQG"
     gene            complement(3432..4235)
                     /locus_tag="VF058_11295"
     CDS             complement(3432..4235)
                     /locus_tag="VF058_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6208927.1"
                     /translation="MSAVAPSRSLLRPPDVRLRSAFRLWQRNATIYRRTYKMNLLPNF
                     FEPFLYLLAMGLGLGAYLSQVEGIRYIDFIAPGLVATAAMFGTSYDVTFNAFVKLRFG
                     KVYDAVMATPLTPEDIALGELLWGTTRAFIYGVVFLIIASFFGVVHSWLALLAPLAIA
                     LVGMMFSVIGLAFTAVVPLIDYFTYYWTLFITPMFLFSGIFFPLDRLPDWVQSVAWFI
                     PLHQAVNLMRALVLHGDPGAAAAAAAWIVVLTAALGVLPLNLLRRRMIR"
     gene            complement(4232..5167)
                     /locus_tag="VF058_11300"
     CDS             complement(4232..5167)
                     /locus_tag="VF058_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6208928.1"
                     /translation="MSTLPGAPVVAARGLVKRYGTFEAVRGIDLDVPAGRCFGFLGPN
                     GAGKTTTMRMIATSAPPSEGELTVLGLPVERGREIRSRMGVVPQENNLDEEITVIQNL
                     VVYARYFDIPPSVAQPRGEELLEFVALGDKRERMPEELSGGMKRRLLIARALMSDPEV
                     LVLDEPTTGLDPQARHLVWEKLRELKRRGVTLLLTTHYMEEASQLCDRLVIMHEGRIL
                     VEGSPRELVAREVPPQVVEVLEPQDHEREHLAALEGVADHVEHLSDRVLLYGNDGEAL
                     LGKVRGIVADQGSVWLRGSGLEDVFLSLTGRRLIE"
     gene            5259..5738
                     /locus_tag="VF058_11305"
     CDS             5259..5738
                     /locus_tag="VF058_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX6208929.1"
                     /translation="MGRALVLNATQEPLAVVTARRAVVLVLKDKAEVLETNGQVFRSE
                     FALFDAPSVVRLNYFVKVPYRARASLTRRAVFARDDWSCQYCGKPAENVDHVVPRSRG
                     GEHVWENVVASCRACNSRKENRLLHEAGLRLRRNPYVPRDGLKLSIGRLEPEWEAYL"
     gene            5835..6197
                     /locus_tag="VF058_11310"
     CDS             5835..6197
                     /locus_tag="VF058_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208930.1"
                     /translation="MLGVVFLTLGTGGCTREPAASPPDPSPAPTVGRDVLKLARSQGL
                     VGCRLAVAYSAPGAESRESILEEIAGDLDADSSEPEDIADAMARELAGPNAAPEVVEA
                     AAVGCLTGLERTLDRLER"
     gene            6392..>6706
                     /locus_tag="VF058_11315"
     CDS             6392..>6706
                     /locus_tag="VF058_11315"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX6208931.1"
                     /translation="MAELVGERRYQMDPKGRISLPVKFRESFDDGVFITLGQDGCLYA
                     FPADEWRRRAEEIDRLPISDPKARDYARIFFGKAEQVDLDSQGRLVVPRQLRETARLG
                     KEV"
BASE COUNT         1024 a         2303 c         2496 g          883 t
ORIGIN      
        1 caggctcccg gggacgatga gcgatccccg ctcggcgtcg tgccgcaggt cgagcccgac
       61 ggcggcgacc tcgacggggg tgtgcgcggc cgggaggtag cggccgccca gcctggtcac
      121 ccggacgtgc tgggtgaggg ccgggccctc gccggggggt tgggggagga ggccctggcc
      181 gccggggacc gctgccgggt cgccccgcgg atcccccggc cgccagaagc gccccgtgta
      241 ctcgtcgagg gcctggagcc tccagtacgc gggccggtcc gcctcgatgg tgaagagctc
      301 gagcggctcg tcgcgcatga ggttcgggcg gatgtccacg agggggctga tcgcgacccg
      361 tcctccggat cccccgtcga ggtcgaggac ggcgtcggcg ccgaagccgg ggaggagccc
      421 cggcaggagc acggccacgg ccgtggcggc gaggcccaac cgccgggcca ggcggccgga
      481 gggcccggcg gcgagccggg cggcggagag ggatcgccgg gggaggaccg gcccccacag
      541 gtcgagctgc ctcatcccgg acgtgtagac cacggccagc gcggcgacca ggaacgcagc
      601 ggcgtacgcg ggtcgcgcac cgtcctcggc cacgacgttg gcgaacccga tcaggcccgc
      661 gggcgggacg agggccaaga tcgggctggc cgcgcgggcc gccagcgcat gggcgccgta
      721 ggccgcggcc cacaccgcga tgatcgatgg agcgaagagc gcggggatgg ccggggcggg
      781 ggcggcctcc tcgaccgccc gccggcccag gttgccgagc gcccggccca gggcgtcgag
      841 ggtggagggg gtggggagcc cgaaccaggt cgtggcgggg aagacgagga gcccgatcgt
      901 gacgagggcg gcggccgccg cggtcgccac cgacagcgcc aggtgtcgcc gctccagcag
      961 cgccgcgagg gcgaccgcgg cggccgccgc caggacgaga cgcgccgccg gccgaccgcc
     1021 ctcgaacacg cgaccgaagg cggcgccggt gacaccggcg agcaggatca gcgccgccgt
     1081 taggagcgcg tggccgctgc gatcggtcgt gcggttccca gtgtccatgc ttcctggagc
     1141 ctccggtccg gggtcaggag gacggtcctc cactccgccc gcgagagacg cacccgggcg
     1201 gaacgaacct cgtcgccgtc cccggccacc acgaggacgg ccaccttctg cccgtatgcg
     1261 ccggtggcgg cgatcagcgc gctcaggtcc tcgtcggcga gcaggccggt gacgaagagg
     1321 agcgccgagc ccgtggcgct gcggtcgcgg agcggaacga gcgggcggcc cagctggcgc
     1381 tcgggggagg gatccgtgag ggccagggcc tcgaggaacg cctcgggcgc catccgctgg
     1441 ggggcgaggt cgacggtccc gagacggagg tcgatcccgc cccggaggta gagcgtcccc
     1501 accgaggccg ccgccgagac ggcgcgctcg aggtcctggt cggccccgta gacggcccgt
     1561 cgcgagtcga ggaacaccgt ggcggaggcc cgccgccccg cctcgtcctg gcggatcatg
     1621 aggcgccccg ttcgggcggt ggacggccag tggatccgcc gcaggtcgtc gccgatctcg
     1681 tactcccgca tcgtgaagaa ctcctcgccc gaccggaaga gctgtcgggc ggtcgtgtcc
     1741 ccagcgcccc cagccgtccg ggcgggggag ggcgcgcgca ggtcgtccac gtgcggatac
     1801 acgatcagct cgtggacgtc ggggaactcc tgcttccgct gggcgagccc gaaggggtcg
     1861 gtcaccgagg cgatgagggg cccgatgtgg aagcggcccc gccgcccgca gtgcacggtg
     1921 tacgagatcg tctggcggtg acgacctcgg aggcctccga gcaccgcgcg cgcggcgcgc
     1981 cccaggctcg ggggcaggcg atcctccagg agcaccaggg aggcggccga ccggctcgtg
     2041 ttctggacct cgatcgccac gcgcagcggc gacccggcga acacgcgacg ctgcccgagc
     2101 cggcgggtgg cccgcagccc gtgccggctc caccggatga agagggccgc cagggcaggg
     2161 agcagcagca ggccgagcgc gaccatatgg aggtcgggcg agcccgtcat gcgtgccgcc
     2221 acccagagga gcgccgcggc ggcgagtacc gagagcccgc gccgcgtcag catgatcagc
     2281 cctggaccgg gacggggacc tcgtccagga ggcccacgag gagcgaggag gccgagtgcc
     2341 cgctcatcgc ggcgtcgggc gcgacgatga ggcggtggga gaggaccggg acgaggagcg
     2401 ccttgacgtc gtcggggagc acgtagtcgc gaccctcgct ggccgcgtag gccctcgccg
     2461 ctcgcatcag catcagggag gcgcgggggc ttgcgccgag gtagaggtcg gggtgcgacc
     2521 gcgtcgcccc caccaggtcg acgatgtagc ggcggatcgg ctccgccacg tggacctcgc
     2581 gcgccatcgc gatgagctcc gcgacaccgc ccgcgtcggc cacgggatcg atgtcctcga
     2641 gcgccgagtg ggacccgtgg gcggtgagga tctcagcctc tgcctcggcc tcggggtacc
     2701 cgatcgagag ccggatcatg aagcggtcga gctgagcctc ggggagcgcg taggtcccct
     2761 cgtgctcgat cgggttctgg gtggcgatca ccatgaaggg caccccaagg gcgtaggtcg
     2821 cgccgtccac cgtcacctgg tgctcctcca tgctctccag caacgcggac tgggtctttg
     2881 ggctggcccg gttgatctcg tcgcccagga cgatgttggc gaagatcgct cccggcttga
     2941 actcgaagtc ctgctgctcc tggttgaaga cgttgacgcc ggtgatgtcc gtggggagga
     3001 ggtcgggggt gaattggatc ctccggaacg agcagtcgat cgagcgggcg atcgccttgg
     3061 ccagcatcgt cttccccacg ccggggacgt cctcgatcag aacgtggccc tccgccacga
     3121 gggccgccag ggccagccgg gtctcccgac tcttcccctg gatcacgtga tcgatgttgg
     3181 ctcggatcgc gtcgaaggtt gcccggaacc gttcccagtc gctgctcgct gccacgcccc
     3241 ctccggagtt gagtgctccg attctacggt gccgagggcg gaaacggcgc ggaggggagg
     3301 cggcggccga cgcccttcat ccctgagacg ccccgcgcgg cccctgtatg ggagcgtccg
     3361 tcatccctca ggcgcggccg gccccgcctc cgataggatt acacgggtgt cattcgcccg
     3421 agtcaagcac ctcagcggat catccggcgg cggagcaggt tgaggggcag cacgccgagc
     3481 gccgccgtca gcacgacgat ccacgcggcc gcggccgccg cggccccggg gtcgccgtgc
     3541 agcaccagcg cccgcatcag gttgacggcc tggtgcagcg ggatgaacca tgccacggac
     3601 tggacccagt ccgggagccg gtccagcggg aagaagatcc cgctgaagag gaacatgggc
     3661 gtgatgaaga gcgtccagta gtaggtgaag tagtcgatca gcggcaccac ggcggtgaac
     3721 gccagtccga tcaccgagaa catcatcccg accagcgcga tggccagcgg cgcaaggagc
     3781 gcgagccacg agtgcaccac cccgaagaac gaggcgatga tcaggaagac cacgccgtag
     3841 atgaaggccc gcgtcgtccc ccagagcagc tcgcccaggg cgatgtcctc gggggtgagc
     3901 ggcgtggcca tgacggcgtc atacaccttg ccgaaccgca gcttcacgaa cgcgttgaat
     3961 gtgacgtcat agctcgtacc gaacatcgcg gcggtggcca cgagcccggg cgcgatgaag
     4021 tcgatgtacc ggatcccctc cacctgcgag aggtaggcgc cgaggcccag ccccatcgcc
     4081 aggaggtaga ggaacggctc gaagaagttc ggcaggaggt tcatcttgta cgtccgccga
     4141 tagatcgtgg cgttccgctg ccacaggcgg aacgcggacc gcaggcggac gtcgggcggg
     4201 cggagcaggc tccggctcgg ggcgacggcg ctcactcgat cagcctcctg cccgtgagcg
     4261 agaggaacac gtcctcgagc ccggagcccc ggagccacac cgagccctgg tcggcgacga
     4321 tcccgcgcac cttgcccagc agcgcctcgc cgtcgttccc gtagaggagc acccgatcgg
     4381 agaggtgctc cacgtggtcc gccacgccct cgagcgcggc caggtgctcc cgctcgtggt
     4441 cctgaggctc cagcacctcc accacctggg gcgggacctc gcgggccacg agctcgcgcg
     4501 gagagccctc cacgaggatc cggccctcgt gcatgatcac gagccggtcg cacagctgcg
     4561 aggcctcctc catgtagtgg gtcgtgagga ggagggtgac gccccggcgc ttcagctccc
     4621 tcagcttctc ccagacgagg tggcgggcct gggggtcgag gcccgtggtg ggctcgtcga
     4681 ggaccaggac ctcggggtcg ctcatcaggg cccgggcgat cagcaggcgg cgcttcatgc
     4741 cgccggacag ctcctcaggc atccgctccc gcttgtcgcc cagcgccacg aactcgagca
     4801 gctcttcgcc gcgcggctgc gccaccgacg gggggatgtc gaagtacctc gcgtacacga
     4861 cgaggttctg gatcacggtg atctcctcgt cgaggttgtt ctcctggggg acgacgccca
     4921 tccgggagcg gatctcgcgt ccccgctcga ccggcagccc gagcacggtg agctcgccct
     4981 cgctgggagg agcggaggtg gcgatcatgc gcatcgtcgt cgtcttcccc gccccgttgg
     5041 ggcccaggaa gccgaagcac cgccccgcag ggacgtcgag gtcgatgccg cggacagctt
     5101 cgaatgtccc gtagcgcttg acgaggccgc gggccgccac gacgggggcg ccggggaggg
     5161 tgctcacggc ggcgagtcta gcaccggccc ccggaacacc cggcggctcc ccctataatg
     5221 gcgacacgcc ccggacgatc accggggtcg gcagaggcgt ggggagagca ctcgtactca
     5281 acgcgacgca ggagcccctc gccgtggtga cggcgaggcg tgccgtcgtg ctcgtgctca
     5341 aggacaaggc cgaggtcctc gagacgaacg gccaggtctt ccgctcggag ttcgccttgt
     5401 tcgacgcccc ctcggtggtg cggctgaact acttcgtcaa ggtgccctac cgggccaggg
     5461 cctcgctcac ccgtcgggcg gtcttcgccc gtgacgactg gtcctgccag tactgcggga
     5521 agccggccga gaacgtggac cacgtggtcc ccaggtctcg cgggggagag cacgtctggg
     5581 agaacgtcgt ggcctcgtgt cgcgcgtgca actcgcggaa ggagaaccgg ctcctgcacg
     5641 aggcggggct gcggctccgg cgaaatccgt acgtcccccg ggacggcctg aagctgtcga
     5701 tcgggcgcct cgagcccgag tgggaggcct acctgtagct ggtcgctggc cccgccgggg
     5761 ccaggtggtt cgtcgaagag gtcaggatgg tgaccacccg gtcacgatcg cgacatgggc
     5821 cgctacgatg cctggtgctg ggggtggtct ttctaacact tgggacgggc ggatgcacgc
     5881 gtgagcccgc cgcctcgccg ccagacccaa gccccgctcc gacggttggc agagacgtgc
     5941 taaagctcgc ccggagccag gggctcgtgg ggtgccggct cgcggtggcg tactcggcgc
     6001 cgggagcgga gtcccgtgag tcgatcctcg aagagatcgc cggggatctg gacgcggaca
     6061 gctccgaacc cgaggacatc gccgatgcga tggcccgaga actcgcgggg ccaaacgctg
     6121 cacccgaggt ggtcgaggcc gccgccgtag gctgcttgac ggggttggag cgaaccttgg
     6181 atcgtctcga gcgctgacct gtccggattg tggccaagcg gtcagcttcc gtaccaacgg
     6241 ctgaccccac ccccgccccc gggcctcctc ccgacaccgc cacatcagcc acacccttta
     6301 acttccatca ctgtaattcc ctccagcatc ttgactgggt ggagtacagg tggcataaac
     6361 gtggggccca gtggagggga ggggagtgcg ggtggccgaa ctcgtcggcg agcgccgata
     6421 ccagatggac cccaagggga ggatctcgct ccccgtgaag ttcagggagt ccttcgacga
     6481 cggcgtcttc atcacgctgg ggcaggacgg ctgcctctac gccttcccgg ccgacgagtg
     6541 gcggcggcgc gccgaggaga tcgaccggct ccccatctcc gacccgaagg cccgcgacta
     6601 cgcgcggatc ttcttcggga aggccgagca ggtggacctg gactcccagg gccgcctggt
     6661 ggtcccgcgc cagctccggg agacggcgcg gctgggcaag gaggtc
//
LOCUS       DASUTW010000328         4243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1796519, whole genome shotgun sequence.
ACCESSION   DASUTW010000328 DASUTW010000000
VERSION     DASUTW010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1796519"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..981
                     /locus_tag="VF058_11320"
                     /pseudo
     CDS             70..981
                     /locus_tag="VF058_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
     gene            1236..1973
                     /locus_tag="VF058_11325"
     CDS             1236..1973
                     /locus_tag="VF058_11325"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019436111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6208932.1"
                     /translation="MSDYEPSTYGDAIADVYDELHEWHLDTDAAIEALARLAGEGPVL
                     ELGVGTGRIAVPLAERGIEVHGIDASESMVEEMRRKPGGEGVRVAIGDFAAVEAPADA
                     YSLVFVVFNTLFALSSQEDQVRCFARVAERLTEDGVFVVEAFVPDVSRFDRGQRVDVS
                     RVETDRVVLDVSMHDPIAQRIVSQHLLLGEGTVRQVPVELRYAWPSELDLMARLAGLR
                     LRSRHGGWREEPFTATGTRHVSIYARA"
     gene            complement(1958..2713)
                     /locus_tag="VF058_11330"
     CDS             complement(1958..2713)
                     /locus_tag="VF058_11330"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX6208933.1"
                     /translation="MEELREAALRGVLEGGRVVREAALKGTRAKGAGDYVTEVDREAE
                     RRIAALLEEATGIGVRGEEHGGEAAGRHWLVDPVDGTTNLVHGFPIVGVSVALIEEGG
                     PVVGAVHAPHLDATWTAVRGAGAERDGRPLAVSSRPTGEAIVATGFPFRHKDRLPEYL
                     RVLVPALQRFEDLRRPGAASLDLAWVAEGTFDGFFELGLSSWDVAAGALLIEEAGGFV
                     TDWAGGDGYMAGDILAGNPEVHAALLELAQALA"
     gene            2988..3359
                     /gene="rpsL"
                     /locus_tag="VF058_11335"
     CDS             2988..3359
                     /gene="rpsL"
                     /locus_tag="VF058_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX6208934.1"
                     /translation="MPTTQQLVRRGRKEPIAKTKSPALTGSPQRRGVCTRVYTTTPKK
                     PNSALRKVARVRLTSGVEVTAYIPGIGHNLQEHSIVLVRGGRVKDIPGVRYKVIRAAL
                     DTAGVSDRKKARSKYGAKKGS"
     gene            3361..3831
                     /gene="rpsG"
                     /locus_tag="VF058_11340"
     CDS             3361..3831
                     /gene="rpsG"
                     /locus_tag="VF058_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX6208935.1"
                     /translation="MPRRGAASKRATVPDPIYQNPLVTQLINKVMRDGKKSLAERVVY
                     DALERISEQTANDPVLTLKKAVENTRPLLEVRSRRVGGATYQVPVEVKPQRGTTLALR
                     WLVGFARQRREPTMAARLVGEIMDASQGQGSAVKRREDIHKMADANRAFAHYRW"
     gene            3938..>4243
                     /locus_tag="VF058_11345"
     CDS             3938..>4243
                     /locus_tag="VF058_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX6208936.1"
                     /translation="MKGQQKTGGQKGLVIREYPLSRTRNIGIMAHIDAGKTTTTERIL
                     YYTGRTYKVGEVHEGTAVMDWMVQEQERGITITSAATTTKWKDHWINIIDTPGHVDFT
                     "
BASE COUNT          687 a         1452 c         1545 g          559 t
ORIGIN      
        1 ccgatggatg agggcgttgc gctcatcacg gaggacgagc tggcgcgcat cgcggactgc
       61 caggcgccgc gcgtctacgt ccgctcccca ttgctctgcg agtcgcggca cggcgtctgc
      121 cgtcgctgct acggctggca cctcggccgc cgggccgtgg tcgagatcgg cgacgcggtc
      181 gggatcgtgg ccgcgcagtc gatcggcgag cccggcacgc agctcacgat gcgaaccttt
      241 cacaccggcg gcgtggccgg ccaggacatc acgcacggcc ttccccgtgt tgtcgagctc
      301 ttcgaggccc gccgccccaa gggcgaggcc cagatcaccg agctctcggg gaaggtcgag
      361 atcgaggagg acgaggagaa gggcgtccgc aggatcacgg tgacgtccga cgacggcgag
      421 caggttcagt acgccgtctc actgcgggcg aggctgaccg tgaccgacgg cgacgtcgtc
      481 gaggtcggcc agcggctcac cgagggatcg gtgaaccctc acgagaagct gcgggtcgag
      541 ggcgtgggcg ccgtgcagcg gcacctcgtg gaggaggtcc aggaggtcta ccggagccag
      601 ggcgtgacga tccacaacaa gcacatcgag ctgatcatcc ggcagatgct gcggaaggtg
      661 cacatcctgg agcccgggaa caccgagttc ctcccgggcg agctcgtcga ccgccggcgc
      721 ttcgaggaga agaacgccga ggtcgtggag ggtggcggcg agccggcgtc ggcccggccg
      781 atgctgatgg gcatcaccaa ggcctcgctg gcgaccgagt cgtggctctc ggcggcgtcg
      841 ttccaggaga cgacgcgggt gctcaccgac gcggcgatca gcgccaagtc cgacccgctg
      901 ctcggcctca aggagaacgt catcatcggc aagctgatcc ccgcggggac cggcatgaac
      961 cgctaccgga acctccaggt gcggatcaac cccgacgcca tcccagagta ctggctggcc
     1021 cgccagcagg cgcccgagga gggcgccgcc caggagggcg agatgaccga ggaggaggcc
     1081 cgccgggttc tcttcggcga gggcgacggg cagggcgcgg gcgaggaggt cccgtccgag
     1141 gtgggcgagg aggcctgagc aggaccgcct gagggcccgg gcgcgcgctg tggcgcgcgc
     1201 ccgggcccct cggcttgggt aggcttggtc ccgccatgag cgactacgag ccctccacct
     1261 acggcgacgc catcgccgac gtctacgacg agcttcacga gtggcacctg gacacggacg
     1321 ccgccatcga ggcgctggcg cggctggcgg gggagggtcc ggtgctcgag ctgggcgtgg
     1381 gcaccgggag gatcgccgtc cccctggccg agcgggggat cgaggtccac gggatcgacg
     1441 cgtccgagtc gatggtcgag gagatgcgcc ggaagcccgg cggcgagggc gtccgggtgg
     1501 cgatcgggga cttcgccgct gtcgaggcgc ccgcggacgc gtactcgctg gtgttcgtcg
     1561 tcttcaacac gctcttcgcc ctctcctcgc aggaggacca ggtccggtgc ttcgcgcgcg
     1621 tggccgagcg gctgaccgag gacggggtct tcgtcgtgga ggccttcgtc ccggatgtct
     1681 cacgattcga tcgtggccag cgggtggacg tctccagggt cgagacggac cgcgtcgtgc
     1741 tcgacgtctc gatgcacgac cccatcgccc agcggatcgt ctcccagcac ctgctcctgg
     1801 gcgaggggac ggtgcggcag gtcccggtgg agctccggta tgcgtggccc tccgagctcg
     1861 acctcatggc gcgtctcgcc gggctgaggc tccgctcccg ccacggcggc tggagggagg
     1921 agcccttcac cgcgaccggc acgcggcacg tctcgatcta cgcgagggcc tgagccagct
     1981 cgagcagggc cgcgtggacc tcggggttcc cggccaggat gtctccggcc atatacccgt
     2041 cgccgcccgc ccagtccgtg acgaacccgc ccgcctcctc gatcagcagc gcccccgccg
     2101 ccacgtccca ggaggacaga ccgagctcga agaagccgtc gaaggtccct tcggcgaccc
     2161 aggccagatc gagcgaggcc gcccccggcc gccgcaggtc ctcgaaccgc tggagggcgg
     2221 ggacgagcac ccgtaggtac tcggggaggc ggtccttgtg acggaagggg aagcccgtgg
     2281 ccacgatcgc ctctccggtc ggccgggacg agaccgccag gggccgtccg tcccgctcgg
     2341 ctccggcgcc ccggaccgcc gtccaggtgg cgtcgagatg aggggcgtgc accgcgccca
     2401 cgaccggccc gccctcctcg atcagggcca cggagacccc cacgatcggg aacccgtgca
     2461 cgaggttcgt cgtgccgtcc accgggtcca cgagccagtg gcgtcccgcg gcctcgcccc
     2521 cgtgctcctc gcctcgcacc ccgatccccg tcgcctcctc gagaagcgcg gcgatcctcc
     2581 gctcggcctc gcggtcgacc tcggtgacgt agtcgcccgc gcccttcgcc cgggtcccct
     2641 tgagggccgc ctccctgacg acccgccctc cttccaggac gccccgcagg gccgcctccc
     2701 gaagctcctc catacgtgga ttcaagcagg ccgggcaggc ggacggaggg gctcggggcc
     2761 gcgtatactg gagcacgtcc cgagcggtcc tcggccgctc gattgacagt cctgaggcac
     2821 ggttataagg ttggcgattg tggtccgggc gcctccgggc catgttcgtc tcgcaacccc
     2881 aggagaaccg aagggccttc ggcgcgaccg cggccctggg accggtctgc cggccgggtt
     2941 ctgagcggtt cctgggtcac ttcgaggaag acgaggacgg gagaggtatg ccgacgaccc
     3001 agcagctggt tcgccggggc cggaaggagc ccattgcgaa gacaaagagc ccggcgctca
     3061 cgggttcccc tcagcgtcgc ggcgtgtgca cgcgcgtgta cacgacgacg ccgaagaagc
     3121 cgaactcggc ccttcggaag gtggcgcgcg tccgtctcac cagcggcgtc gaggtcacgg
     3181 cctacatccc cggcatcggg cacaacctcc aggagcactc gatcgtcctg gttcggggcg
     3241 gccgggtgaa ggacatcccg ggcgtccgct acaaggtgat ccgggcggcc ctcgacactg
     3301 cgggcgtctc cgaccggaag aaggcgcgct cgaagtacgg cgccaagaag gggagctgag
     3361 gtgcccagac gaggagccgc ctcgaagcgg gccaccgtgc ccgacccgat ctaccagaac
     3421 cctctggtta cccagctcat caacaaggtg atgcgggacg ggaagaagag cctcgccgag
     3481 cgcgtcgtgt acgacgcgct cgagcggatc agcgagcaga ccgcgaacga tccggtgctg
     3541 acactgaaga aggcggtcga gaacacccgg ccgctgctgg aggtgcgctc ccgtcgggtg
     3601 gggggcgcga cctaccaggt gcccgtcgag gtgaaaccgc agcgggggac gaccctcgcg
     3661 ctccggtggc tggtggggtt cgcccgccag cggcgggagc ccacgatggc ggcgcgcctc
     3721 gtgggcgaga tcatggacgc ctcccagggg caggggtcgg cggtgaagcg ccgggaggac
     3781 atccacaaga tggccgacgc gaacagggcg ttcgcgcact acaggtggtg atcgtggaag
     3841 ggcatccgcg aggcccggac gaccgggccc gaggcgaggg gaggtcgtag tggccgtccc
     3901 cgcgaggaac atggacatgg cacagccggc ggaacagatg aaggggcagc agaagacggg
     3961 tggccagaag ggcctggtca tccgggaata ccccctgtcc aggacccgga acatcgggat
     4021 catggcgcac atcgacgcgg gcaagaccac gaccaccgag cggatcctct actacacggg
     4081 ccggacctac aaggtcggtg aagtccacga ggggaccgcc gtcatggact ggatggtcca
     4141 ggagcaggag cgggggatca cgatcacctc cgccgccacc accaccaagt ggaaggacca
     4201 ctggatcaac atcatcgata cgccgggcca cgtggacttc acc
//
LOCUS       DASUTW010000329         3505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1539126, whole genome shotgun sequence.
ACCESSION   DASUTW010000329 DASUTW010000000
VERSION     DASUTW010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539126"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /gene="sucB"
                     /locus_tag="VF058_11350"
     CDS             <1..1384
                     /gene="sucB"
                     /locus_tag="VF058_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HEX6208937.1"
                     /translation="QLGETVVEGTIVKWLKSEGDSVDRDEPLFEISTDKVDTEVPSPI
                     AGTITEILVQEGETVEVGTQLAMIESGDGQAAAEGAPSEEAEEPKEQAQAGEEKAEEP
                     PRPEPEPEPAPQPEQQPAQPERQEAQPERQPAQPAAAAERGPRSHILSPLVRRLADEH
                     GIDLDRVQGTGTGGRITKKDIMAVVESGQPAEEPAAAEARPPLQAVPEEPRPAPAPQA
                     APAAAEEVRPISHIRKRIAENMVASLHQSARAWNAVEVNMERIAQLRNRAKDAFKRQE
                     GISLTYMPFLARAVSGALLAYPDVNARLNLEEGTATYRRSVNLGVAVAYDEGLIVPIV
                     KGAEGMNVVGLARAINDVATRARSKKLSPDEVHGGTFSITNPGPFGSLISVPIMNPGE
                     TAILAFDTVQKRPVVIDDAIAIRHMVYLSLSWDHRMIDGATAAQFLARVKENLETWDF
                     TSEVQAYLSE"
     gene            1381..2067
                     /gene="lipB"
                     /locus_tag="VF058_11355"
     CDS             1381..2067
                     /gene="lipB"
                     /locus_tag="VF058_11355"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX6208938.1"
                     /translation="MTSEAWLLTPGRVPYGPALEAMRELVDRRIAGDVPDSLLLLEHE
                     PVYTAGRRTDPSHLLMAERDLRARGAELHHVDRGGSVTFHGPGQLVGYPIVHLGVAPS
                     LAEHVRRMQDVVIRSCADVGVRAAPDDEVGVWCGDRKVCAIGVKSTRRVTYHGFALNC
                     TTDLSWFDAIVPCGLADRGVTTLSELAGRTVTTHEMTPIVARRFAEVFDRELSPAPAE
                     AAPALGRAVA"
     gene            complement(2074..2613)
                     /locus_tag="VF058_11360"
     CDS             complement(2074..2613)
                     /locus_tag="VF058_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208939.1"
                     /translation="MRCVLVLVAAAVAAAAPVRAQQQGPEEPTIAEVEPARARVGDTV
                     DVRGTGWEPGSRIEISHQRRPLAFATVRPDGRWEAGVRVPSADPPTTIEVTVSGTAVT
                     GETTTLEVSLDVAPGMPRGIWIVVGGVALVVLLVLALGWAMLARQRRRSITQRVAEGQ
                     TPSRNPDPRREPQPHVGRR"
     gene            complement(2653..3324)
                     /locus_tag="VF058_11365"
     CDS             complement(2653..3324)
                     /locus_tag="VF058_11365"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX6208940.1"
                     /translation="MAWEDLPPPSETRPRDLLADVGRAQRAVKLLIGPFLRWYVSMEV
                     TGAEDVPGDGPAILAPNHESMWDVPLLAVASPRPVVFMAKEEVFSGPIKSRFFTLLGG
                     FPVRRGGRDFDAMRRSLAAVRSGRVLCLYPEGTRHRGAELGPFLSGAAWIALAEGVPL
                     VPVGILGTDEIWSPGSLVPRRAAVRVAFGEPIRVERARRPAARRTRADELTMELRNRV
                     AALLA"
     gene            3323..>3505
                     /locus_tag="VF058_11370"
     CDS             3323..>3505
                     /locus_tag="VF058_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208941.1"
                     /translation="MAGRVYRPRWGPLPGALLESGARALAAAGRVTARRLFRVATKTQ
                     EDRHSELGLSADDLQAM"
BASE COUNT          529 a         1292 c         1281 g          403 t
ORIGIN      
        1 gcagctgggc gagaccgtcg tcgaggggac gatcgtcaag tggctgaaga gcgaggggga
       61 cagcgtcgac cgcgatgagc ccctgttcga gatctcgacc gacaaggtgg acaccgaggt
      121 gccctcgccc atcgccggca ccatcaccga gatcctcgtg caggagggcg agaccgtcga
      181 ggtcgggacc cagctggcga tgatcgagtc gggggacggg caggccgccg cggagggcgc
      241 gccgtccgag gaggccgagg agccgaagga gcaggcccag gccggggagg agaaggccga
      301 agagccacct cggcccgagc ccgagccgga accggcccct cagccggagc agcagcccgc
      361 ccagcctgag cgccaggagg cccagcctga gcggcagccc gcccagccgg cagccgcggc
      421 cgagcgaggc ccccggagcc acatcctctc ccccctcgtc cggcggctcg cagacgagca
      481 tgggatcgac ctggaccggg tccagggcac cggcacgggg ggaaggatca cgaagaagga
      541 catcatggcg gtcgtggaat cggggcagcc cgccgaggag ccggccgcgg cggaggcccg
      601 gcccccgctc caggcggtcc ccgaggagcc gcggccggct ccggcccccc aggcggcgcc
      661 cgcggccgcc gaggaggtcc ggccgatctc gcacatccgc aagcggatcg ccgagaacat
      721 ggtggcgagc ctccaccagt ccgcgcgggc ctggaacgcc gtcgaggtca acatggagcg
      781 gatcgcgcag ctccggaacc gggccaagga cgcgttcaag cggcaggagg gcatctcgct
      841 cacctacatg ccgttcctgg cccgggcggt gagcggcgcg ctgctggcct accccgacgt
      901 caacgcgcgg ctgaacctcg aggagggcac cgccacctac cggcggtcgg tgaacctcgg
      961 ggtggccgtg gcctacgacg aggggctcat cgtccccatc gtgaaggggg ccgaggggat
     1021 gaatgtggtg gggctggcac gcgcgatcaa cgacgtggcc acgcgggccc gctccaagaa
     1081 gctctcgccg gacgaggtgc acggcgggac cttctcgatc acgaaccccg ggccgttcgg
     1141 gtcgctgatc tccgtcccca tcatgaaccc gggcgagacc gcgatcctgg cgttcgacac
     1201 ggtgcagaag cggcccgtgg tgatcgacga tgcgatcgcg atccggcaca tggtctacct
     1261 ctctctgtcc tgggaccacc ggatgatcga cggggccacg gccgcgcagt tcctggccag
     1321 ggtgaaggag aacctcgaga cctgggactt cacgagcgag gtccaggcct acctgagcga
     1381 gtgacgagcg aggcctggct gctgacgccg gggagggtgc cctacggccc cgctctcgag
     1441 gcgatgcggg agctcgtgga tcgccgcatc gccggggacg tccccgactc gctgctgctg
     1501 ctggagcacg agccggtcta cacggcgggg cggcggacgg acccctcgca cctgctcatg
     1561 gccgagcgcg acctgcgggc ccggggagcg gagctccatc acgtggatcg gggcggcagc
     1621 gtgacgttcc acgggccggg ccagctcgtg ggctacccga tcgtccacct cggcgtggcg
     1681 ccgagcctgg cggagcacgt gcggcggatg caggatgtcg tgatccggtc gtgcgccgac
     1741 gtcggggtgc gcgccgcccc cgacgacgag gtcggcgtct ggtgcggcga tcgaaaggtg
     1801 tgcgcgatcg gggtgaagag cacgaggcgc gtcacctacc acggcttcgc gctcaactgc
     1861 acgaccgacc tctcgtggtt cgacgcgatc gtgccctgcg ggctggccga ccgcggcgtg
     1921 acgaccctgt cggagctcgc cggcaggacc gtcaccaccc acgagatgac cccgatcgtg
     1981 gcccggaggt tcgccgaggt cttcgatcgc gagctgtccc ccgcgcccgc ggaggccgcc
     2041 ccggcgctcg gccgggccgt ggcctgagcg gcctcagcgc cgcccgacgt gcggctgagg
     2101 ctccctccgg ggatcggggt tccgcgacgg ggtctggccc tcggccaccc gctgagtgat
     2161 cgacctccga cgctggcgcg cgagcatcgc ccagcccagg gcgagcacga ggaggacgac
     2221 gagcgccacg ccgcccacca cgatccagat ccctcgaggc atgcccggcg ccacgtcgag
     2281 ggagacctcg agcgtcgtgg tctcccccgt gacggcggtc ccgctgaccg tcacctcgat
     2341 cgtcgtcggc gggtcggccg aggggacccg cacccccgct tcccaacgac cgtccggccg
     2401 gacggtggcg aaggccagtg ggcggcgctg atgggagatc tcgatccggc tccccggctc
     2461 ccagccggtg ccgcggacat ccacggtgtc gcccacccgg gcccgggcgg gctccacctc
     2521 ggcgatcgtc ggctcctcgg ggccctgctg ctgggcccgg acgggagccg ccgcggccac
     2581 cgcggccgcc acgaggacga gaacgcacct catgctctca gcggtacccg gggcgccggg
     2641 aggggaaacg gttcaggcaa gcagggcggc cacccggttc cgcagctcca tggtgagctc
     2701 gtcggccctg gtgcggcgcg cggccggccg ccgcgcgcgc tccacgcgga tcggctcgcc
     2761 gaaggcgacg cggacggccg cgcgccgcgg caccagcgat cccggggacc agatctcgtc
     2821 cgtccctagg atccccacgg gcacgagcgg caccccctcg gccagcgcga tccaggccgc
     2881 ccccgacagg aacggcccca gctcggcccc ccggtggcgg gtcccctccg ggtagaggca
     2941 cagcacccgg cccgagcgga cagccgccag cgagcggcgc atcgcgtcga agtcccggcc
     3001 cccccgccgg acagggaacc cgcccagcag cgtgaagaac ctggacttga tcggccccga
     3061 gaagacctcc tccttcgcca tgaagaccac cgggcggggc gaggccacgg ccagcagcgg
     3121 cacgtcccac atcgactcgt ggttgggagc gaggatcgcc gggccgtccc cgggcacgtc
     3181 ctcggccccc gtcacctcca tggagacgta ccagcgcagg aacggcccga tgagcagctt
     3241 cactgcccgc tgagcccgtc ccacgtcggc cagcaggtcc cgggggcgcg tctcgctcgg
     3301 cggcggcaga tcctcccagg ccatggcggg acgagtctac cggccccgct ggggccccct
     3361 cccgggggcc ctgctagaat ccggcgcacg ggccctcgcg gccgccggcc gggtaaccgc
     3421 aaggaggttg ttccgcgtgg cgacgaagac ccaggaagac cgccattccg agctcggcct
     3481 ctccgcagac gacctgcagg ccatg
//
LOCUS       DASUTW010000330         3725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1539497, whole genome shotgun sequence.
ACCESSION   DASUTW010000330 DASUTW010000000
VERSION     DASUTW010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539497"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VF058_11375"
     CDS             <1..751
                     /locus_tag="VF058_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208942.1"
                     /translation="AWGDVYDEKLWADPEATVARIADRGARTLYLQTASTTTKRRMIR
                     FPEATARFLDAARRRGIRTVAWYLPELLGAPKDLRRAVAAATFTTENGARFDGVALDI
                     EATHEPDPRLRVDRLLELSRQVRGILGSDYPLGAIVPSPIRGPDFWPVLPVAELAKVY
                     DAILPMAYWTFHTSGEDAAHGFISQSIRMLREEAGPEVPIHVIGGIADTSPGELRGFV
                     RAVREAEVVGASVYDVDTTDERGWRVLRELT"
     gene            790..1233
                     /locus_tag="VF058_11380"
     CDS             790..1233
                     /locus_tag="VF058_11380"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX6208943.1"
                     /translation="MRPEGIIEMLRERGYRITPQREAIIEEVLKAEGHITPQSLARRL
                     HRTMPAVNASTVYRTMALLEEVGVVQHSHLERGAEYHRIGEGDHVHLTCTRCGAGDDL
                     SLDEADELRELIRRHRGFRADLTHFAIGGLCETCQAEAQPTAGSR"
     gene            complement(1209..2051)
                     /locus_tag="VF058_11385"
     CDS             complement(1209..2051)
                     /locus_tag="VF058_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX6208944.1"
                     /translation="MSTLVEPFSFEFFRNGILVATLSGILTGLVGVYVVLRGMSYIGH
                     GLSHAIFGGAVASFVTNVNFYLGAGAWGVVATLMINRVARRRTIGADAAIGVVTSASL
                     AFGLALLSRFRSFTRNFEAALFGNVLGVELADLAVVGGVTLAAAVFVFMLYRPLLFAT
                     FDPEVAEASGVRTGWIDAGFAVVLAATVVATMQILGVLLIAAMLVIPPVIGRLLTDSF
                     GKMLGLSVLVGGLSGFTGMYLSWYLNISSGATVVLVAAAVFVVVYAVSGARRRLPQRE
                     PAVG"
     gene            complement(2035..2835)
                     /locus_tag="VF058_11390"
     CDS             complement(2035..2835)
                     /locus_tag="VF058_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX6208945.1"
                     /translation="MMDELIRMEKVTAAYGQDPVLVNVDLAVGPGDFVGIVGPSGSGK
                     TSVLRVMTGSLRPVAGRVAKAPRLRIGYVPQVDTVNWYFPVTVREAVLMGRTGDRRLP
                     WASREELRLADEVLEHLGIAEVAGRHIRELSGGQQQRIFVARAMLRRPQLLLLDEPTS
                     GVDVRTRHDVLHLLHELHHDGLAIVVTTHDLNGVAAHLPELVCLHREVIARGRPREVL
                     TPEVLERTYGAPLEVLVHGGMPVVVDPAAIAKEEKVVPMEGGHHEHAG"
     gene            complement(2835..>3725)
                     /locus_tag="VF058_11395"
     CDS             complement(2835..>3725)
                     /locus_tag="VF058_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX6208946.1"
                     /translation="TVSPITNIVQNVGGDRVQVTGIVPEGQNSHTFEPAPTDARVMAE
                     ADVVFVNGLNLELPTMELATANTRDDVEIVELGGETITPDEYIFDFGFPREDGDPNPH
                     LWTNPPYALRYAEIVRDRLIALDPEGRETYERNFDAFAERLDELDRLVREVTETVPEE
                     NRKLLTYHDSFPYFARKYGWEIIGAIQPSDFADPTPQEVARLIEQVRAQGVPAIFGSE
                     VFPSPVLERIAAETGARYVADLRDDDLPGSHGDPDHSYLGLMIFDYTTFMGALGGDPS
                     AFEQLDVSNVAGECCATYRE"
BASE COUNT          588 a         1350 c         1283 g          504 t
ORIGIN      
        1 cgcctggggg gacgtctacg acgagaagct ctgggcggac ccggaggcca ccgtggcccg
       61 gatcgccgat cggggggccc gcaccctgta tctccagacg gcgagcacga ccacgaagcg
      121 gcggatgatc cgcttcccgg aggccaccgc ccggttcctc gacgccgccc gccggcgagg
      181 catccggacg gtggcctggt acctccccga gctcctgggg gccccgaagg acctccgacg
      241 cgcggtcgcg gcggccacgt tcacgaccga gaacggcgcg cgcttcgacg gcgtcgccct
      301 cgacatcgag gcgacgcacg agcccgaccc gcggctgcgc gtggaccggc tcctggagct
      361 ctcccgacag gtgcgaggga tcctcgggtc ggactacccg ctcggagcga tcgtcccctc
      421 gccgatccgc gggccggact tctggcccgt ccttcccgtc gccgagctgg cgaaggtcta
      481 cgacgcgatc ctccccatgg cgtactggac gttccacacc tccggcgagg acgccgccca
      541 cgggttcatc tcgcagagca tccggatgct gcgggaggag gccggtcccg aggtgccgat
      601 ccacgtgatc ggggggatcg cggacacgtc gcccggcgag ctccgaggct tcgtccgagc
      661 cgtgcgggag gcggaggtgg tcggggccag cgtctacgac gtcgacacga ccgacgagcg
      721 cggctggcgc gtcctccgag agctgacctg agcggggcac ctgccacgct cttgcagtag
      781 cctggggccg tgcgacccga agggatcatc gagatgctgc gggagcgcgg ctaccgcatc
      841 acgccccagc gcgaggccat catcgaggag gtcctgaagg ccgaggggca catcacgccc
      901 cagtcgctgg cccgccggct ccaccggacg atgcccgccg tcaacgcctc gaccgtgtac
      961 cggacgatgg ctctcctgga ggaggtcggc gtcgtccagc actcgcacct cgaacgcgga
     1021 gccgagtacc accggatcgg cgagggggac cacgtgcatc tgacgtgcac gcgttgcggg
     1081 gccggggacg acctctccct cgacgaggcc gacgagctac gcgagctgat ccgccgccac
     1141 cgcgggttcc gcgccgacct gacccacttc gcgatcggcg ggctgtgcga gacctgccag
     1201 gccgaagctc aaccgacggc cggctcgcgc tgaggcagcc gccgcctcgc tcccgagacc
     1261 gcgtagacca ccacgaagac cgcggcggcc acgagcacga ccgtggcccc cgacgagatg
     1321 ttgaggtacc aggagaggta catccccgtg aaccccgaga gcccccccac gagcaccgag
     1381 aggcccagca tcttcccgaa cgagtcggtc agcaggcgtc cgatcaccgg cgggatcacc
     1441 agcatcgccg cgatcagcag caccccgagg atctgcatcg ttgccacgac cgtggcggcc
     1501 agcacgaccg cgaaccccgc gtcgatccac ccggtgcgaa ccccgctcgc ctccgccacc
     1561 tccgggtcga acgtggcgaa cagcagcggc cggtagagca tgaacacgaa gaccgccgca
     1621 gccagcgtga ccccgcccac caccgcgagg tccgcgagct ccacccccag cacgttgccg
     1681 aacagcgccg cctcgaagtt cctggtgaag ctccggaagc gcgacaacag cgcaagcccg
     1741 aacgccaggc tggccgaggt caccacgccg atcgcagcat cggccccgat cgtgcgccgc
     1801 cgcgcaactc ggttgatcat cagcgtggcc acgacgcccc acgcccccgc cccgaggtag
     1861 aagttcacgt tcgtcacgaa gctggccacg gccccgccga agatcgcgtg tgacagcccg
     1921 tggccgatgt acgacatgcc gcggagcacc acgtaaacgc cgacgagccc cgtcaggatc
     1981 cccgacagcg tcgccaccaa aatcccgttc cggaagaact cgaacgagaa cggctcaacc
     2041 agcgtgctca tggtgccctc cctccatcgg gacgaccttc tcctccttgg cgatcgcggc
     2101 cgggtccacg accacgggca tgccgccgtg gaccagcacc tcaagcggcg cgccgtaggt
     2161 ccgctccaac acctcgggag tcaggacctc cctggggcgc ccgcgggcga tcacctcgcg
     2221 gtggaggcac acgagctcgg gcaggtgcgc ggccacgccg ttgaggtcgt gcgtggtgac
     2281 gacgatggca aggccgtcgt ggtgcagctc gtggaggagg tggaggacgt cgtggcgggt
     2341 gcgcacgtcc acgccactgg tgggctcgtc gagcaggagg agctggggac gccgcagcat
     2401 ggcccgggcc acgaagatcc gctgctgctg gccgccggac agctcgcgta tgtggcggcc
     2461 cgcgacctcg gcgatcccca ggtgctccag cacctcgtcc gccagccgca gctcctcgcg
     2521 tgaggcccac gggaggcggc ggtcgccggt ccgccccatc agcacggcct cgcgcaccgt
     2581 caccgggaag taccagttca cggtgtcgac ctggggcacg tagccgatcc gaagcctcgg
     2641 cgccttcgcc acgcgcccgg ccaccggccg gagcgacccc gtcatcaccc gcaggaccga
     2701 ggtcttcccc gagccgctgg gcccgacgat cccgacgaag tcgcccggcc ccacggccag
     2761 gtccacgttc acgaggaccg ggtcctgccc gtaggccgcg gtgaccttct ccatcctgat
     2821 cagctcgtcc atcatcactc ccggtaggtc gcgcagcact cgcccgccac gttcgagacg
     2881 tcgagctgct cgaaggcgct cgggtcgccg cccagcgccc ccatgaacgt cgtgtagtcg
     2941 aagatcatca gcccgaggta ggagtggtcg ggatcgccgt ggcttccggg caggtcgtcg
     3001 tcgcggaggt cggccacgta ccgggccccg gtctcggccg cgatccgctc cagcacgggg
     3061 ctggggaaga cctccgaccc gaagatggcc ggcacgccct gggcgcggac ctgctcgatc
     3121 aggcgggcca cctcctgggg ggtggggtcg gcgaagtccg agggctggat cgccccgatg
     3181 atctcccatc cgtacttgcg agcgaagtac gggaacgagt cgtggtaggt cagcagcttg
     3241 cggttctcct cggggacggt ctcggtgacc tcgcgcacga ggcggtccag ctcgtcgagg
     3301 cgctccgcga acgcgtcgaa gttgcgctcg taggtctcgc gaccctcggg gtcgagggcg
     3361 atcaggcggt ctcggacgat ctcggcgtag cggagggcat agggcgggtt ggtccagagg
     3421 tgcgggttgg ggtcgccatc ctcccgcggg aagccgaagt cgaagatgta ctcgtcgggg
     3481 gtgatcgtct cgccgccgag ctccacgatc tcgacgtcat cgcgggtgtt ggccgtggcc
     3541 agctccatcg tggggagctc gaggttgagg ccgttgacga agacgacgtc cgcctcggcc
     3601 atgacgcggg cgtcggtggg cgcgggctcg aacgtgtggc tgttctggcc ctcgggcacg
     3661 atgccggtga cctggacgcg gtcgcccccc acgttctgga cgatgttcgt gatgggggag
     3721 acggt
//
LOCUS       DASUTW010000331         2403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1539696, whole genome shotgun sequence.
ACCESSION   DASUTW010000331 DASUTW010000000
VERSION     DASUTW010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539696"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="VF058_11400"
     CDS             <1..1325
                     /locus_tag="VF058_11400"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020629371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX6208947.1"
                     /translation="GGLRSFWDYGPLGVELKRNVRAAWWKDMVQERDNVVGLESSIMM
                     SPKVWEASGHLESFTDPLVECMSCHQRFRADHLPGFHAPESGHAEGEEGTVDMAGAKP
                     PACPNCGARDWTDPRNFNLMFKSFMGPIEAEENVVYLRPETAQGMFVDFVTVQAAARK
                     KLPFGIAQIGKSFRNEITPGNFIYRTREFEQMEMEFFVEPGTDEEWHEYWIEQRLGWY
                     TGLGIREENLRVREHEKDELSHYSKRTVDVEYAFPFTDWGELEGIANRTDFDLKAHEK
                     MSGQDLTYFDQEKDERYHPYVIEPAAGVDRAALAFLVDAYREEEAPSAKGGTEKRVVL
                     KLHKDLAPIKVAVLPLSRNEKLVPKAREVFDLLKGEWMCDYDDAGSIGRRYRRQDEVG
                     TPFCLTVDFDTLDDNAVTVRDRDTMRQERVGLAGLMSHLSGSLREVKR"
     gene            complement(1580..1777)
                     /locus_tag="VF058_11405"
     CDS             complement(1580..1777)
                     /locus_tag="VF058_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208948.1"
                     /translation="MERLGTMSHEHELLKRDMQILFAELRVEIAERFDAQTKTLFRTF
                     IVSNAVMILSVAGLAFGITLA"
     gene            1950..2120
                     /locus_tag="VF058_11410"
     CDS             1950..2120
                     /locus_tag="VF058_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208949.1"
                     /translation="MGERSRSRTAGRLRAVLLRPFRRRTERPDVTEEEHRLRREMSSR
                     RRRYDPDPPWMS"
BASE COUNT          429 a          764 c          817 g          393 t
ORIGIN      
        1 acggtgggct ccgctcgttc tgggactacg gccccctcgg ggtcgagctg aagcgcaacg
       61 tccgcgcggc ctggtggaag gacatggtcc aggagcgcga caacgtcgtg ggcctcgagt
      121 cctcgatcat gatgtcgccg aaggtctggg aggccagcgg gcacctcgag tccttcacgg
      181 accccctcgt cgagtgcatg agctgccacc agcggttccg cgccgaccac ctccccggct
      241 tccacgcccc cgagtcgggc cacgcagagg gcgaggaggg gaccgtggac atggccggcg
      301 ccaagccccc cgcctgcccc aactgtggtg ctcgcgactg gacggacccg cgcaacttca
      361 atctgatgtt caaatccttc atggggccga tcgaggccga ggagaacgtc gtctacctgc
      421 gccccgagac ggcccagggg atgttcgtgg acttcgtgac cgtccaggcg gccgcgcgga
      481 agaagctccc gttcgggatc gcccagatcg gcaagtcgtt ccgcaacgag atcacgcccg
      541 gcaacttcat ctaccggacg cgcgagttcg agcagatgga gatggagttc ttcgtcgagc
      601 ccggcaccga cgaggagtgg cacgagtact ggatcgagca gcggctcggc tggtacacgg
      661 gcctcgggat ccgcgaggag aacctgcggg tccgcgagca cgagaaggac gagctctcgc
      721 actactcgaa gcggacggtg gacgtcgagt acgcgttccc gttcaccgac tggggcgagc
      781 tcgaggggat cgccaaccgg accgacttcg acctcaaggc gcacgagaag atgtcgggtc
      841 aggacctgac gtacttcgac caggagaaag acgagcgcta ccacccgtac gtgatcgagc
      901 cggcggcggg ggtggaccgg gccgcgctcg cgttcctcgt ggacgcgtac cgcgaggagg
      961 aggccccgtc ggccaagggg gggaccgaga agcgcgtggt gctgaagctg cacaaggacc
     1021 tcgcgccgat caaggtcgcc gtcctgccgc tgtcacggaa cgagaaactg gtcccgaagg
     1081 cgcgcgaggt cttcgacctg ctcaagggcg agtggatgtg cgactacgac gacgccggct
     1141 cgatcgggcg gcggtaccgg aggcaggacg aggtcggcac gcccttctgc ctcacggtcg
     1201 acttcgacac cctcgacgac aacgcggtga ccgtccgcga ccgcgacacc atgcgacagg
     1261 aacgtgtcgg cctcgctgga ctcatgagcc atctgtcagg atcgctgcgt gaggtcaaga
     1321 ggtgagtgaa acatcgattt ctccgtggac actgcggcat cctttgcggg actggcaacg
     1381 agatgcgttc cgcctatgga agaagaacgg gagacgtggc gttgtccggg tcgtcacggg
     1441 cgcaggaaag acttaccttg atcgtctccg tcgacctctt cccgatcgcg ttctcgagcg
     1501 agctgtagag ctcgtgtcgg tcggactcgc tcaccgccat tgtgtccctc ccttccacgg
     1561 gcaacaagag ggggagggtt caggcgaggg tgatgccgaa ggcgaggccg gccaccgaga
     1621 ggatcatgac cgcgttggag acgatgaacg tccggaagag ggtcttcgtc tgggcatcga
     1681 aacgctccgc gatctccacc ctgagctcgg cgaacaggat ctgcatgtcc ctcttcagca
     1741 gctcgtgctc gtggctcatc gtgccgagtc gctccatcag cgtgtgcgcc tcctcggtgc
     1801 ccaggacctc cccgagtcgt ctgcgtagcc gttcctcgcg ttcatgtagc aggcattgtg
     1861 tccctcctgc tccgagccaa caagaggggg acggagcgag cgtactcgga ggcgccgaca
     1921 taggatcgac gaggcacctg ggagggatca tgggcgagcg atcgagatct cgcaccgcgg
     1981 ggaggcttcg ggccgtcctt ctgcgcccct tccgacgccg gaccgagcgc cccgacgtca
     2041 ccgaggagga gcatcgcctg cgccgcgaga tgtcctccag gcgccgccgc tacgaccccg
     2101 accccccctg gatgtcctga tccgatgttt cttcgtctgg cttgggaggg attcagccgg
     2161 gccgcctgga caggggggag tactgttgta agtagagctt ggccggaaca cgttggccca
     2221 tcggcccgcc ctcgcgccga gctcgtcaag gagagatgct ttcatgaaga ccttcatcgt
     2281 gcacggcaac aaccgtgccg gggagttggc gaagatcacc ggcgcgctct cgtcgaaggg
     2341 cgtgaacgtg ctgatcagcg ccttcggaga gaacggtcgg gccctcgcag gcttcgtcgc
     2401 gag
//
LOCUS       DASUTW010000332         4045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1020698, whole genome shotgun sequence.
ACCESSION   DASUTW010000332 DASUTW010000000
VERSION     DASUTW010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020698"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..96
                     /locus_tag="VF058_11415"
     CDS             <1..96
                     /locus_tag="VF058_11415"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006750002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEX6208950.1"
                     /translation="GEGSGALLAVPLVRAAAAAVTDVATFEEWGL"
     gene            93..896
                     /locus_tag="VF058_11420"
     CDS             93..896
                     /locus_tag="VF058_11420"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEX6208951.1"
                     /translation="MSPGRGLRGALAFLTRVPVDRGHGAREVAGSVPWFPVAGALVGL
                     LVAGVYAAGASVLPSLPAAVVAVAAGVLATGALHEDGLADVADALGARGPDDARRIMK
                     DPTHGTFGVLALTLSVAARAAAIASLGPWAALAWVPAAHAASRGAAVGLLARARTGPR
                     RGLAAEEADRREPGLGAGTAAHLPERAGWAALSIGLAASALGVGFWAVPVGALVLAAT
                     AYLGWLAARRLGGVTGDVMGAAQQVGEVLVLLLGAAAVTQGWPGAVWWR"
     gene            896..1429
                     /locus_tag="VF058_11425"
     CDS             896..1429
                     /locus_tag="VF058_11425"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6208952.1"
                     /translation="MVEVPPGVRLWLVRHGSTAWSEEGRLCGRSDMPLSPRGRLEAQA
                     LRDAAARLTPAGVWSSDLRRARETAELAGLFALSDARLRELDFGSLEGARWDDLPAAV
                     REAMLVFDGFRAPGGESVSDLARRVEGFLDDLAPGLHVLVTHGGVIRLLMRGRGPEAH
                     VAPGGAIRLEAQPAARS"
     gene            complement(1408..1860)
                     /locus_tag="VF058_11430"
     CDS             complement(1408..1860)
                     /locus_tag="VF058_11430"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6208953.1"
                     /translation="MKEPTTMEPVRVAVTVLTRPDDAFRLYTERIADWWPLETHSLFG
                     RDATRAVFEGREGGRIYEVHRDGRESEWGRVLVWDPPARVVHSWQPNPERSGTTEVEV
                     RFHEVDGGTRVELEHRGWERLGDEAEALRGEYEGGWVTVLDAYERAAG"
     gene            complement(1857..2189)
                     /locus_tag="VF058_11435"
     CDS             complement(1857..2189)
                     /locus_tag="VF058_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020521453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6208954.1"
                     /translation="MANQVLEALGDGTRQAILDILLDGPRSVAQLSERLPVSRPAVSQ
                     HLRILARAGLVGYTTRGTRHLYHVHPEGLAELRAYVEQFWARALAAFEARAADEGSSE
                     EDRKERSR"
     gene            complement(2357..3238)
                     /locus_tag="VF058_11440"
     CDS             complement(2357..3238)
                     /locus_tag="VF058_11440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6208955.1"
                     /translation="MTERAYERDETRVASGDLAVATGDSMRLFLNEIGRYPLLTADEE
                     KELAKLVEDGDQEAMDRMIRSNLRLVVSVAKKYQWAGLPLLDLIQEGILGLIRAVEKF
                     DWRKGFKFSTYATWWIRQAISRAIDNQSRTIRLPAHLAQRELKISRARRQLEEELGRE
                     PTIEETAEASGIDAEEIERVRDAARAVTSLERPVDADGETELGDLVAGENAEIEEEVH
                     VRLRDTSVRRAVASLSDTQREVVALRYGMSGEDPLSYREIAKRMGMSHEAVRRVERDA
                     LKNLAVLREIEALEEAV"
     gene            3568..3783
                     /locus_tag="VF058_11445"
     CDS             3568..3783
                     /locus_tag="VF058_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208956.1"
                     /translation="MARDEMRRDRVDETIRDDLREPDRQHLDEQTASGLPEGGPGRSK
                     MENLRRIRRQRETQRRRLREADPEARV"
     gene            3931..>4045
                     /locus_tag="VF058_11450"
     CDS             3931..>4045
                     /locus_tag="VF058_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003601584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX6208957.1"
                     /translation="MATGTVKWFSPDKGYGFISQEDGPDVFVHFSAIMGDGY"
BASE COUNT          504 a         1462 c         1472 g          607 t
ORIGIN      
        1 ggggagggaa gcggggccct gctcgcggtg cccctggttc gcgccgccgc cgcggccgtc
       61 accgacgtgg ccaccttcga ggagtggggc ctgtgagccc cggccgaggg ctccgggggg
      121 cgctcgcctt cctgaccagg gtcccggtcg atcgcggcca cggcgctcgg gaggtggccg
      181 gatcggtccc ctggttcccg gtggcggggg cgctcgtcgg gctgctcgtg gcgggcgtgt
      241 acgcggcggg ggcgtcggtg ctcccatccc tgccggcggc ggtcgtggcc gtggccgcgg
      301 gtgtcctcgc cacgggcgct ctccacgagg acgggctggc cgacgtggcc gacgcgttgg
      361 gcgcgcgggg ccctgacgac gcccgccgga ttatgaagga cccgacgcac gggaccttcg
      421 gagtgctcgc cctgaccctg tcggtcgcgg ctcgtgcggc cgcgatcgcc tcgctcggcc
      481 cgtgggcggc gctggcctgg gtgccggccg cgcacgccgc gagcagggga gcggccgtgg
      541 gcctgctggc tcgcgcccgg acaggccctc gtcggggtct cgccgccgag gaggccgacc
      601 gccgcgagcc gggcctcggc gctgggaccg ccgcccacct ccccgagcgg gcggggtggg
      661 cggccctgag cataggcctg gcggcgtcgg cgctcggcgt cggcttctgg gccgtccccg
      721 tcggggcgct cgtgctggcg gcgaccgcct acctcgggtg gctcgccgcc cgcaggctgg
      781 gcggggtgac cggagacgtg atgggggcgg cgcagcaggt gggggaggtg ctggtgctgc
      841 tgctcggcgc cgcggccgtc acccagggct ggccgggagc cgtctggtgg cggtgatggt
      901 ggaggtcccc cccggcgtcc ggctctggct cgtccgccac gggtccacgg cctggagcga
      961 ggagggccgg ttgtgcggcc gctccgatat gccgctcagc ccgcggggcc gtctcgaggc
     1021 ccaggccctc cgcgacgcgg cggcgcgact caccccagcg ggggtgtggt cgtcggacct
     1081 ccgccgggcg cgggagaccg ccgagctggc ggggctgttc gccctctccg acgcaaggct
     1141 cagggagctc gatttcggct cgctggaggg ggcgcgatgg gacgatctcc cagccgccgt
     1201 ccgcgaggcc atgctcgtgt tcgacggctt ccgggcgccc ggcggcgagt cggtgtcgga
     1261 cctcgcgcgg agggtcgagg gcttcctgga cgatctggcg ccggggctgc acgtcctcgt
     1321 cacgcacgga ggcgtgatca ggctgctcat gcggggtcgg ggcccggagg cgcacgtcgc
     1381 tccgggcggg gcgatccgtc tggaggctca gcccgccgcg cgctcgtagg catccagcac
     1441 ggtcacccag cccccctcgt actcgccgcg gagtgcttcg gcctcgtccc cgaggcgctc
     1501 ccatccgcgg tgctcgagct cgacgcgggt cccgccgtcg acctcgtgga accggacctc
     1561 gacctcggtc gtccccgacc gctccgggtt cggctgccac gagtgcacga ccctggccgg
     1621 tggatcccac acgaggacgc ggccccactc ggactcccgc ccgtcccggt gcacctcgta
     1681 gatgcgcccg ccttcccggc cctcgaagac ggcccgggtc gcgtcccgcc cgaagaggga
     1741 gtgtgtctcg agcggccacc agtcggcgat ccgctcggtg tagaggcgga acgcgtcgtc
     1801 gggccgcgtg agcaccgtca cggcgacccg gaccggttcc atcgtggtcg gctccttcat
     1861 cgtgatcgct ccttccgatc ctcctcggag gacccttcgt cggcggcgcg ggcttcgaac
     1921 gcggccagcg cgcgggccca gaactgctcg acgtaggcgc gcagctcggc cagtccctcg
     1981 ggatggacgt ggtagaggtg gcgcgtcccg cgggtcgtgt agccgacgag cccggctcgg
     2041 gccaggatgc gcaggtgctg agagacggcc ggcctgctca ccggcagccg ctccgagagc
     2101 tgcgcgaccg atcgggggcc gtccagcagg atgtcgagga tggcctggcg ggtcccgtct
     2161 ccgagcgcct cgagcacttg gttcgccatc acgaacggta agttatcgcg aacggtaagc
     2221 gaatgtcaac ggaaaactcg agggccacgg ttccacaacg gaaacgggcg cgcccctcct
     2281 cggggcgcgc ccgttcacat ggtggtcggg ccctggaacg atctacccta gccctccgct
     2341 cgacgggagg gcaagctcag accgcctcct ccagggcctc gatctctcgc aggacggcca
     2401 ggttcttcag cgcgtcgcgc tccacccgcc tgacggcctc gtgcgacatg cccatccgct
     2461 tggcgatctc ccggtacgac agcggatcct cgcccgacat cccgtagcgg agcgcgacca
     2521 cctcgcgctg cgtgtccgag agcgaggcca cggctcgccg cacgctcgtg tcgcgcagcc
     2581 gcacgtggac ctcctcctcg atctcggcgt tctcgccggc cacgaggtcg cccagctccg
     2641 tctcgccgtc cgcgtccacc ggacgctcaa ggctcgtcac cgcgcgggcc gcgtcgcgga
     2701 cgcgctcgat ctcctcggcg tcgatgcccg acgcctcggc ggtctcttcg atcgtcggct
     2761 cccggcccag ctcctcctcg agctggcgcc gggcccgcga gatcttcagc tcgcgctgag
     2821 cgaggtgcgc ggggagccgg atcgtgcgcg actggttgtc gatggcccgg gagatggcct
     2881 gccggatcca ccacgtcgca tacgtggaga acttgaagcc cttccgccag tcgaacttct
     2941 cgaccgctcg gatcaggccg aggatcccct cctggatcag gtccaggagc gggaggcccg
     3001 cccactggta cttcttcgcg accgagacga ccagccggag gttcgaccgg atcatccggt
     3061 ccatcgcctc ctgatcgccg tcctcgacga gcttggcgag ctccttctcc tcgtccgcgg
     3121 tcagcagcgg gtaccggccg atctcgttga ggaacagccg catgctgtcg ccggtggcca
     3181 cggccaggtc gccgctcgcc accctcgtct cgtcacgttc gtatgcacgc tcggtcatgg
     3241 gtctccaaac gtcgtcgaaa cggcccctct cccatgctct ccacgcgagg ggccctgaaa
     3301 cctgcgccgc ggggggacgc attcctcatc tatcttcgac tcgatccgtc caacggttca
     3361 gtggtgggcc gtattcccga cgccgaaccg ccgatggccg ttccggatcc gctgccggcc
     3421 gtgacgtcga agagtgccag tggaacccgg acaacgagga cgaacccccg ggggaggctg
     3481 cgtcctcatc gccctccggg tctagccggg cagcgtcgtc aggccgacct gagtgtcggt
     3541 cccagggagg cggggtacgc atcgagtatg gctcgggacg agatgcgccg ggatcgggtc
     3601 gacgagacga tccgggacga cctccgcgag ccggaccggc agcacctcga cgagcagacc
     3661 gcgtcgggcc tccccgaggg cggcccgggc cggtcgaaga tggagaacct ccggaggatc
     3721 cgccggcagc gcgagaccca gcggcgccgt ctccgcgagg ccgacccgga ggcccgggtc
     3781 taggcccgag agcgcctcac tggtagactc taggtgaggg ttccgcatgt ggtttcacgc
     3841 ttcaggcagt tccctcaccg gcgcgacggc gccgggggcc gacggacgag cagttcacgt
     3901 ccgacccggc cacgaaagga gggaacgctt gtggcaacag gaaccgtgaa gtggttcagc
     3961 ccagacaagg gctacggctt catctcccag gaggacggtc ccgacgtctt cgtgcacttc
     4021 agcgcgatca tgggcgatgg gtacc
//
LOCUS       DASUTW010000333        13995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_114582, whole genome shotgun sequence.
ACCESSION   DASUTW010000333 DASUTW010000000
VERSION     DASUTW010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13995
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114582"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1495)
                     /locus_tag="VF058_11455"
     CDS             complement(149..1495)
                     /locus_tag="VF058_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HEX6208958.1"
                     /translation="MRKRILLHIVAVAVVATALSASPLLAGDQEEAVQEFGSRFDAVV
                     ANEAALNANAPTSHAPCIDGMSAGEFPCDNVDMLSFVPHGALGTTFVNDIWGWTDPQT
                     GKDYALLGSSEGTVFVDISDPKRPQVLGILPTHSTAGGAFWRDIKVYENHAFVVSEHS
                     NHGMQVFDLTQLRDWDGTYTIYSNTAHYSVFGRAHNININEDTGFAYAVGARQGEGCN
                     FGLHMIDISDPTNPTFAGCFGDNGYVHDTQCVIYEGPDAEHRGKEICFNSNAPGAYGS
                     TNHWVDIVDVTDKANPVSLGRSDYPLSGYSHQGWLTPDQATFLHGDEGDEFIHGLGTT
                     TRVWDVSDLENPTLIGVHDNGTTSIDHNVYTEGRLAFASNYTTGLRVWDTRDAADGEL
                     TELGYFDVYPENDNASFEGGTWSNFTYFSQKKVVAVSSIDRGLFILRPRGGLNNND"
     gene            complement(1718..2263)
                     /locus_tag="VF058_11460"
     CDS             complement(1718..2263)
                     /locus_tag="VF058_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208959.1"
                     /translation="MSESRSGGPWRYGVGLAACAALVWTGLISGSRVPALALVDLGFH
                     ELGHLVAAPLPDLITALAGSVSQIAVPVGLAAYFLFAQRDLLGVGLCLTWAGTSARNV
                     AVYVADAPHQALPLIGGQHDWGFILAGSLDLAPAIAGFVTFVAWGLALAGMGVCALGL
                     ARATAQSSKTRFGESSIPPIG"
     gene            2366..2800
                     /locus_tag="VF058_11465"
     CDS             2366..2800
                     /locus_tag="VF058_11465"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HEX6208960.1"
                     /translation="MAFSSELLTVLVIVALGLAVLSLFLLAPPSATLEGQADAIRRLQ
                     VSVEELARADARLREHLAASVQRVGLIRYDAFEDMGGHLSFSLALLDEAGDGVVVSAI
                     NGRQDTRVYAKPVRAGESGHNLSDEEAQAIRQAMAGSPAVSA"
     gene            2805..4073
                     /gene="serS"
                     /locus_tag="VF058_11470"
     CDS             2805..4073
                     /gene="serS"
                     /locus_tag="VF058_11470"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014910424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX6208961.1"
                     /translation="MLDLRSLRDDPEPVKAALARRDPDLPAEVDRLLALDEERRRLTV
                     RADELRAEQNRASKAIGAAEGEERDRLIAEVRRVSDELKEVEPRLVDVDEGIGALLAR
                     LPNPPHESVPQGETDEDNEVVSVVGEPPDLGFEPRDHVDLGTALGLLDLERAARTSGS
                     RFVYLTGDAVLLEWALVRYCLDVLMPEGFVPVIPPVLVREEAMYGTGFLPTDEAQLYV
                     TRDDDLYLVGTSEVPLAAFHQGEILEDFPRRYVGYSTCFRREAGAHGKDTRGMFRVHQ
                     FDKVEMFVFVPPDASEEEHERLLALEERIVSSLELPYRVVDVCTGELGASAARKFDIE
                     VWLPGQGRYRELTSCSNTTDYQARRLGVRMRGPRGNVPVHTLNGTACAVGRTLIAILE
                     NHQRGDGSVGVPDVLHPYLPEGLRVLGPPG"
     gene            4084..5121
                     /locus_tag="VF058_11475"
     CDS             4084..5121
                     /locus_tag="VF058_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020518204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6208962.1"
                     /translation="MEYRVLGRTGVRVSAYCLGTMMFGSWGNPDADECVKMIHRAMDA
                     GINFIDTADVYSKGESEEIVGKALSVRRDQVVLATKVHGRMGQKPNEGGSSRLWIMRE
                     VERSLRRLGTDYLDLYQVHRPDPSVDLEETLGALTHLVRQGKVRYAGCSAYPAWQIVE
                     SHWVSERRGLERFVTEQPPYSIFVRGIERDVLPVTRRYGMGVLVYSPLNGGWLAGRYR
                     KGEENPPGSRAELMKEPGRFGERYDREREPVRRKLDLVEGLEEVAEGAGLSLARMAMA
                     FVLAHPDVTSAIVGPRTPEQLEDLLEGADARLDDSILDAIDAVVPPGTVVDDADRGWE
                     GPWMEAAARRR"
     gene            5118..6362
                     /locus_tag="VF058_11480"
     CDS             5118..6362
                     /locus_tag="VF058_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208963.1"
                     /translation="MTRRGALAALIAVAVLVPPSAALAARDLGPYRGLGSWVDIFDHR
                     DLRDPVGTVKAMRARGVRTVYLETSNFSQPRAIVHRRATVRFLHAAHARGMKVVAWYL
                     PGLQKLRRDRLRSLAAIRFRTAKGHRFDSFALDIESSLVDPPDRRCRRLLRLSRQLRK
                     RALPGYTLGAIIPAPRGIQLAKGYWPRFPYRRLARIYDVFVPMGYYTYRYEGREAARE
                     YTRRNVQILRRRTGDPTIPIHMIGGLSGESSRGEVRGFVRAVREYGLLGGSLYDFRGT
                     RRRHWEELAAVPVNPRQDVPLPAPLSHSGPLGNVPGVDVQHPKEVTFKVGKRAGSWNL
                     TYEAYDAGPGEVEILVNWKRLGEVVPTPPDAWGPSATVPIPDRLLREDARNLIHFVAT
                     GDHPSWSTWGVRGVSLSAAPGP"
     gene            complement(6359..6631)
                     /locus_tag="VF058_11485"
     CDS             complement(6359..6631)
                     /locus_tag="VF058_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208964.1"
                     /translation="MDARYRWFVGPSEPESLAEVVDLLDRRRGFVLAHWGAEDVAGHE
                     AGVRVEVGDRAYVEMLRESDGSLHQRLDPILERAVRARPLSLLDLV"
     gene            6684..6770
                     /locus_tag="VF058_11490"
     tRNA            6684..6770
                     /locus_tag="VF058_11490"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6718..6720,aa:Ser,seq:tga)
     gene            6816..7166
                     /locus_tag="VF058_11495"
     CDS             6816..7166
                     /locus_tag="VF058_11495"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEX6208965.1"
                     /translation="MAKLLINVTHGPEHPTRAALGFLVGSAAADAGHEVSLFLAGDAV
                     QLLREPVLDHLVGLGTGSLRESYDKLREAGARFYVSGMSSQARGFEPDGIAEPAMPGK
                     LVELALEADRVLTY"
     regulatory      7208..7269
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            7282..7656
                     /gene="crcB"
                     /locus_tag="VF058_11500"
     CDS             7282..7656
                     /gene="crcB"
                     /locus_tag="VF058_11500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX6208966.1"
                     /translation="MATALIVGVAGAIGTLARYGLDGLVSRRFPGAFPLGTFVVNITG
                     ALLLGLVFTVLTERFMVSPSVRSAITIGLLGAYTTFSTLSLETLRLIEDGAYLIAAAN
                     ALGTLAVGLVAVYVGVVLGRAV"
     gene            7656..8003
                     /locus_tag="VF058_11505"
     CDS             7656..8003
                     /locus_tag="VF058_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEX6208967.1"
                     /translation="MRIEGEGKLLRIFIGESDTWHGKPLYQAIVERAREEGLAGATVI
                     RGIEGFGAHSRIHTARILRLSEDLPVIIEIVDSEERIRGILPALDEMVTDGLLTMERV
                     EVITYRAGDEAEG"
     gene            complement(8029..8808)
                     /gene="xth"
                     /locus_tag="VF058_11510"
     CDS             complement(8029..8808)
                     /gene="xth"
                     /locus_tag="VF058_11510"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX6208968.1"
                     /translation="MRLISWNVNSIRMRLARTEALLRRHEPDVLCLQELKTAEDTFPG
                     DAFEALGYRTAVHGQPGRNGVAILSREPLKDVAPGFPSDPAPEQARVVSATVEGIRVI
                     CVYVVNGVEVGAPQYELKLTWLDALSSWLRTEHDPSDPLLVIGDFNVAPRDLDVHDPE
                     KWRGRNLASEPERERVRELLGWGLVDLMRLHEDGPGPFTFWDYRAGAFHRGWGLRLDL
                     ALATRPIAERCERVWVERDERKPTFGEGKPSDHAPLIVELG"
     gene            complement(8818..9555)
                     /locus_tag="VF058_11515"
     CDS             complement(8818..9555)
                     /locus_tag="VF058_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014109967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX6208969.1"
                     /translation="MSTLGWILVGGLAMSVIALAGSLTLVLPRRALRRMLLPLVGFAA
                     GALIGGALFHMLPSAAEELSDPIVVYLWFIGGFVSFYVLEQFLHWHHCHRGADCERQP
                     MGTLILAADGLHNLIGGLAVGASFVLATPVGISAWMAAAAHEVPQELGDFGVLVHSGW
                     GPRRALLANLVSALTFPLGALAAYWAAGALEIAFLVPFAAGNFVYIGATDLIPQLTVT
                     EDWREKVVHTIALLLGLGLLLVTAVGG"
     gene            complement(9565..9975)
                     /locus_tag="VF058_11520"
     CDS             complement(9565..9975)
                     /locus_tag="VF058_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208970.1"
                     /translation="MTETKQQRRQTNLMHVLAYAAVAAAVGLFVFLAFLCPERRAIGM
                     IPMALWVPLILWFFSALRRRQALEVVVAFPISFITLILTVLGICPGGAAQSAAIIGLT
                     GFVVYGLVLAASVSSFERWGQEEEEEPRSGTETG"
     gene            complement(9972..11009)
                     /locus_tag="VF058_11525"
     CDS             complement(9972..11009)
                     /locus_tag="VF058_11525"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX6208971.1"
                     /translation="MTVRSPSAEKAGAEKGGGAFRGIKLLLCENPLPPLPEAIAAATQ
                     ALPEANYYTEPYSAPLRGLLAERLRVPEPLLHINAGSELILRQLFDRFGERVHLLTPT
                     YPLFPEIAHSSTETRLRPEEDFAFDLSGLRVPEGTTLVVIVNANNPTGTVFDMGPLPD
                     LLRAHPEVRFLVDEAFIDFAGPSVAGLVPKHPNLLVTRTFSKAQSLAGLRVGYAVLPE
                     EVADDLNANNDAYPLTLPSQAAAVATLQHDQQIKARIEDLRGWAKDLAASLRGMGIRT
                     FPTSAYFFLADLSPYDAHEVAERLEERGILIKPLDDPRLGPGYMRVTTARPEDDERFG
                     SALGEVLKELS"
     gene            complement(11308..12309)
                     /locus_tag="VF058_11530"
     CDS             complement(11308..12309)
                     /locus_tag="VF058_11530"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208972.1"
                     /translation="MSLWQWLLLFAAGAVVLVWAGSVLARSGDEIATRTRLGGLFVGT
                     LLMAAATSLPEIVTVVAAAATEAVDLAVGSIFGSSMGNMAILAVIDLVHRKHVWPAVE
                     LGHARVGTLAIALTSLAVLGVVTPRGPMLGWVGIDTILILLAWIAAMAWMRRSRHGRF
                     ADYPAPTEWGEAESPRGELRSAVVRFSLASLAVLLAGPVVAIAGDGIAETSGLSATFV
                     GVLFVAVATSLPELVASLGALRIGAYDLAVGNLFGSNAFNMIVLVFADAAYTGGPLLT
                     GAGASQVAAGVGAIGLMAVALAAIVHGEETRISRLEPDALVLLIFYLGSLYAVWATS"
     gene            complement(12322..12678)
                     /locus_tag="VF058_11535"
     CDS             complement(12322..12678)
                     /locus_tag="VF058_11535"
                     /inference="COORDINATES: protein motif:HMM:NF025601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HEX6208973.1"
                     /translation="MAEEKVWTEKVKVQADALREKVEELLHEGNVRRIVVKNSEGQTV
                     MELPLTFGIVGAVAAPMITAVGAMAGVAARWSLEIERREPKKTAGAKSAAKKTSSKKT
                     AAKKSAAKKSTSKKAE"
     gene            complement(12703..>13995)
                     /locus_tag="VF058_11540"
     CDS             complement(12703..>13995)
                     /locus_tag="VF058_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012891389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX6208974.1"
                     /translation="LPLCRTASGAPHALARMSERGLRVIAVAARDLAGGPGPATAEEA
                     ERELNLLALIGLEDPPRPEAAEAIAACRRAGIHVAMITGDHPTTAATIADETGLRAPG
                     APVLVGSDLPPDDGALGELVDRDGVVIGRVDPEDKLRIARALRKRGHIVAMTGDGVND
                     GPALHEADIGVAMGRSGTDVAREAADLVLLDDDFATIVTAVEEGRAIFANAQRFLTYH
                     LTDNVAELTPFLVWALSGGSFPLALGVLQILALDLGTDTLSAVALGAEPAEPRTLEQL
                     PARGRLLDGGVARRAFGVLGPVEGLMGMAAFVVSLLAWGWRPGDPFPTGAPLLAASGA
                     TFMAVVIGQTANAFACRSATRWPGALGWLTNRLLVVGAAIELLLSLGFLLIPPVAELL
                     RHAAPTLAGWGVALLTGPAVLAADALHKAAGRRARHDG"
BASE COUNT         2014 a         4925 c         4975 g         2081 t
ORIGIN      
        1 cgctccgcac gcgctgtccg aaccaccagc ggtgcccctc ggcgtcgagc gcctcgtagg
       61 tgcgatcctc ccacgcgtcg tagtcgcggt tcgagggctc gctcacgatg cgcgcgccgc
      121 aggcgcgcgc ccgcgccccc caaccgcctc agtcgttgtt gttcagaccg ccccgcggcc
      181 gcaggatgaa cagcccgcgg tcgatgctgc tgacggccac gaccttcttc tgcgagaagt
      241 acgtgaagtt gctccacgtc cctccctcga aggaggcgtt gtcgttctcc gggtacacgt
      301 cgaagtaccc gagctctgtg agctcaccat cggcggcgtc ccgggtgtcc cacacccgga
      361 ggccggtcgt gtagttcgac gcgaaggcca ggcggccctc cgtgtacacg ttgtggtcga
      421 tcgacgtcgt gccgttgtcg tgcacgccga tcagggtggg gttctcgagg tcggagacgt
      481 cccacacccg cgtggtcgtg ccgaggccgt ggatgaactc atcgccctcg tccccgtgga
      541 ggaaggtcgc ctggtccggc gtgagccagc cctggtgcga gtagccggac aatgggtagt
      601 cggacctccc cagcgagacg gggttcgcct tgtcggtcac gtccacgatg tcgacccagt
      661 ggttcgtgct gccgtacgca ccgggggcgt tggagttgaa gcagatctcc ttgccgcggt
      721 gctcggcgtc cggcccctcg tagatcacgc actgcgtgtc gtggacgtag ccgttgtccc
      781 cgaagcatcc ggcgaaggtc gggttggtcg ggtcgctgat gtcgatcatg tgcagcccga
      841 agttgcaccc ctcgccctgc cgggcgccca cggcgtacgc gaacccagtg tcctcattga
      901 tgttgatgtt gtgcgcccga ccgaacacgc tgtagtgcgc cgtgttcgag tagatggtgt
      961 acgtcccgtc ccagtcccgg agctgggtca ggtcgaagac ctgcatcccg tggttgctgt
     1021 gctccgagac gacgaaggcg tggttctcgt acaccttgat gtcgcgccag aacgcccctc
     1081 cggcggtgga gtgcgtcggc aggatgccca gtacctgggg ccgcttgggg tcgctgatgt
     1141 cgacgaacac ggtcccctcg ctcgacccga gcagcgcgta gtccttgccg gtctgggggt
     1201 cggtccatcc ccagatgtcg ttgacgaacg tcgtgccgag cgccccgtgg ggcacgaagc
     1261 tcagcatgtc gacgttgtcg cacgggaact ccccggccga catgccgtcg atgcagggcg
     1321 catggctcgt cggcgcgttg gcgttcaggg ccgcctcgtt cgccaccacg gcgtcgaagc
     1381 gggagccgaa ctcctgcacc gcctcttcct gatcgccggc gagcagcggc gacgcggaga
     1441 gcgccgtggc gaccacggct accgcgacga tatgcagcag gatcctcttc ctcatgccgt
     1501 cctccttctg gcgtgacccc cgggggcgtc cggacggtcg agcctcgtgg gtcggtcaac
     1561 cggcgttcgt cgctcctcgg gaatcacctc ctcggggtga ggggacagcg caggaagcgt
     1621 gggaggattc gggcacgccg agccggcggt gaggcacttc gtgggcgatg ggagttcgaa
     1681 ggctcagtct cgggctcctt cgtgggagta tagcccgcta ccctatcgga gggatgctcg
     1741 attccccaaa tcgggtcttc gagctctggg cggtcgcccg ggccaggccg agcgcgcaga
     1801 cccccatccc ggccagggcc agcccccacg ccacgaacgt gacgaaaccg gcgatcgccg
     1861 gggccagatc gaggctgcct gccaggatga atccccagtc gtgctgccct ccgatcagag
     1921 gaagggcctg gtggggggcg tcggccacgt agacggcgac gttccgggcc gaggtgccgg
     1981 cccaggtcag gcagagcccg acgccgagca ggtcccgctg ggcgaagagg aagtacgcgg
     2041 ccaggccaac cgggacggcg atctgcgaca ccgagcccgc gagggccgtg atcaggtcgg
     2101 ggaggggggc ggccacgagg tgccccagct cgtggaaccc gaggtcgacc agggcgagcg
     2161 ccgggaccct ggacccggag atcagccccg tccagacgag agcggcacag gctgcaaggc
     2221 ccacgccgta cctccacgga ccacccgagc gcgactcgct cacgtggagg gtatcggcag
     2281 gcgatcggcc gccgctgagg ggatcagccg ggcgggaccg gatcggcccg ggtcggcgcg
     2341 gacccggcgt atactccccg catccatggc cttctccagc gagcttctga ccgtccttgt
     2401 gatcgtggcc ctcgggctgg cggtcctctc cctcttcctg ctggcgccgc cgtcggccac
     2461 cctcgaggga caggccgacg cgatccgccg cctccaggtc tcggtcgagg agctggcccg
     2521 ggccgacgcc cgcctccgag agcacctcgc ggcgtcggtg cagcgggtgg gcctgatccg
     2581 ctacgacgcg ttcgaggaca tgggggggca cctgtcgttc tcgctggcgc tgctcgacga
     2641 ggcgggcgac ggcgtggtcg tgagcgcgat caacggcaga caggacaccc gcgtctacgc
     2701 caagccggtc cgggcgggcg agtccggcca caacctgtcc gacgaggagg ctcaggccat
     2761 ccgccaggcg atggccggat ccccggccgt gagcgcctga ccccgtgctc gacctgcggt
     2821 ccctccgcga cgaccccgag ccggtgaagg ccgcgctggc ccgtcgcgac cccgatcttc
     2881 cggccgaggt ggaccgcctc ctcgccctgg acgaggagcg ccgccgcctg accgtccggg
     2941 ccgacgagct gagggccgag cagaaccggg cctcgaaggc gatcggcgcc gccgaggggg
     3001 aggagcggga tcgcctgatc gccgaggtcc gccgggtctc ggatgagctg aaggaggtcg
     3061 agccccggct ggtcgacgtc gacgagggga tcggagccct gctggcgcgt ctgcccaacc
     3121 ccccgcacga gagcgtgccc cagggggaga ccgacgagga caacgaggtg gtttcggtcg
     3181 tgggcgagcc gcccgacctc gggttcgagc cgagggacca cgtggacctg gggaccgcgc
     3241 tcggcctgct cgacctggaa cgggccgccc ggacctcggg ctcgcgcttc gtctacctga
     3301 ccggggacgc ggtgctgctc gagtgggcgc tcgtccgcta ctgcctcgac gtgctcatgc
     3361 ccgaggggtt cgtcccggtg atcccgcccg tgctggtccg ggaggaggcg atgtacggca
     3421 ccgggttcct ccccaccgat gaggcccagc tctacgtgac ccgcgacgac gacctgtacc
     3481 tggtgggcac ctccgaggtc ccgctggcgg ccttccacca gggagagatc ctggaggatt
     3541 tcccgcgtcg ctacgtgggc tactcgacgt gcttccggcg cgaggccggc gcccacggca
     3601 aggacacccg cgggatgttc cgcgtccacc agttcgacaa ggtggagatg ttcgtcttcg
     3661 tgccgccgga cgcgtcggag gaggagcacg agcgcctcct ggcgctcgag gagcggatcg
     3721 tgagcagcct cgagcttccg taccgcgtgg tcgacgtctg caccggtgag ctgggcgcgt
     3781 cggcggccag gaagttcgac atcgaggtct ggctgcccgg ccagggccgg taccgtgagc
     3841 tgacctcctg ctccaacacc accgactacc aggcccggcg cctcggggtc cggatgcgcg
     3901 gccctcgggg gaacgtgccc gtccacacgc tgaacggcac cgcgtgcgcc gtcggtcgga
     3961 cgctcatcgc gatcctcgag aaccaccagc gcggggacgg ctcggtcggc gtgccggacg
     4021 tcctccaccc gtacctgccc gagggtcttc gcgtcctcgg gccgcccggc taggctcctg
     4081 gccatggagt accgggtcct cggccgcacc ggcgtccgcg tcagcgccta ctgcctgggc
     4141 acgatgatgt tcggctcgtg gggcaacccc gatgccgacg agtgcgtgaa gatgatccac
     4201 cgcgcgatgg acgcgggcat caacttcatc gacacggccg acgtctactc gaagggagag
     4261 tccgaggaga tcgtggggaa ggcgctctcg gtgcggcgcg accaggtcgt gctggccacg
     4321 aaggtccacg gtcggatggg ccagaagccg aacgagggcg gcagctccag actgtggatc
     4381 atgcgggagg tcgagcggag cctccggcgg ctggggaccg actacctcga cctctaccag
     4441 gtccaccggc ccgacccgag cgtcgacctc gaggagacgc tgggcgccct cacccacctc
     4501 gtccgccagg ggaaggtccg gtacgcgggg tgctccgcgt accccgcctg gcagatcgtc
     4561 gagtcgcact gggtcagcga gcggaggggc ctggagcggt tcgtcaccga gcagcctcca
     4621 tactcgatct tcgtccgggg catcgagcgc gacgtcctgc cggtgacccg tcggtacggc
     4681 atgggggttc tcgtctacag cccgctcaac ggggggtggc tggccggccg gtaccggaag
     4741 ggagaggaga acccgcccgg ctcgcgggcg gagctgatga aggagcctgg tcgcttcggc
     4801 gagcggtacg atcgcgagcg ggagccggtc cggcggaagc tcgacctcgt ggaagggctc
     4861 gaggaggtcg cggagggcgc cgggctctcc ctggcccgga tggcgatggc cttcgtgctg
     4921 gcgcaccccg acgtgacctc ggccatcgtc gggccccgca cccccgagca gctcgaggat
     4981 ctcctggagg gcgccgacgc gaggctcgat gactcgatcc tcgacgccat cgacgcggtc
     5041 gtgcccccgg gcactgtggt ggacgatgcc gaccggggct gggaggggcc gtggatggag
     5101 gcggccgcgc gtcgtcgatg acccggcggg gagccctcgc cgcgctgatc gcggtcgcgg
     5161 tgctcgtgcc gccgtccgcc gcgctcgccg cccgggacct cggaccgtac cggggcctcg
     5221 gctcctgggt ggacatcttc gatcatcgtg atctccgcga tcccgtgggg acggtgaagg
     5281 cgatgcgcgc gcgcggcgtc cgcaccgtgt acctggagac gtcgaatttc tcgcagccca
     5341 gggccatcgt ccaccgtagg gcgaccgtcc gattcctcca tgcggcccac gccaggggga
     5401 tgaaggtcgt ggcctggtac ctcccgggcc tccagaagct ccggagggac cggctgcggt
     5461 cgctggccgc gatccgcttc cgcacggcga aggggcaccg cttcgattcc ttcgctctgg
     5521 acatcgagtc gtccttggtc gacccgcccg accgccgctg caggcggctg ctgcgccttt
     5581 cgcgacagct gcgaaagagg gcgctcccgg gctacacgct gggcgcgatc atcccggctc
     5641 cccgcgggat ccagctggcg aagggctact ggccccgctt cccctaccga aggctggcgc
     5701 gcatctacga cgtgttcgtc ccgatgggct actacacgta ccggtacgag ggtcgcgagg
     5761 ccgcccgcga gtacacgcgg cgcaacgtcc agatcctccg ccggaggacg ggggacccga
     5821 ccattccgat ccacatgatc gggggtctct ccggcgagtc gagccgcggc gaggtccgcg
     5881 gcttcgtccg ggcggtccgg gagtacggtc tgctcggggg gagcctctac gatttccgcg
     5941 gaacccgccg gcgccactgg gaggagctgg cggccgtccc cgtgaacccc cggcaggacg
     6001 tcccgctccc cgcgccgctt tcccactcag gccccctcgg gaacgtcccc ggcgtcgacg
     6061 tccagcatcc gaaggaggtc accttcaagg tggggaagag ggcggggtcg tggaacctca
     6121 cgtacgaggc ctatgacgcc ggccccggcg aggtggagat cctcgtcaac tggaagcggc
     6181 tgggcgaggt ggtccccacc ccgcccgacg cctggggccc gtcggcgacg gtccccatcc
     6241 cggaccgcct gctccgggag gacgcccgca acctgatcca cttcgtggcg accggtgacc
     6301 atccgtcgtg gagcacgtgg ggggtgcgag gcgtctcgct gtccgcggcg cctggcccct
     6361 agaccaggtc gaggagcgag agcggccggg cccggaccgc ccgctccagg atcggatcca
     6421 gccgctggtg taggctcccg tcgctctcgc ggagcatctc gacgtaggcg cggtcgccga
     6481 cctcgaccct caccccggcc tcgtgcccgg cgacgtcctc ggctccccag tgggcgagca
     6541 cgaaccctcg tcgccggtcc aggaggtcca cgacctcggc cagcgactcg ggctcgctgg
     6601 ggcccacgaa ccaccggtac cgggcgtcca tcgctccagt atggggcgtg aggaggcgct
     6661 tggaggggag gctacactgc cctgggggga tgccggagcg gacgaacggg acggtcttga
     6721 aaaccgttgg tcccgcgagg ggcccctggg ttcgaatccc agtccccccg ctcgcaagct
     6781 cgccccgtgg tctcggctct cctatcatct gctgtatggc gaagctcctg atcaacgtga
     6841 cgcacggtcc cgagcatccc acgcgtgcgg cgctcggctt cctcgtcggc agcgcggcgg
     6901 cggacgccgg gcacgaggtc tcgctcttcc tcgccgggga cgccgtccag ctgctccgcg
     6961 agcccgtcct cgaccacctc gtgggtctgg gcaccgggag tctccgcgag tcctacgaca
     7021 agctgaggga ggcgggggcg cggttctacg tctcggggat gtcgagccag gctcgcggct
     7081 tcgagccgga cggcatcgcg gagccggcca tgccggggaa gctggtcgag ctggcgctgg
     7141 aggccgaccg ggtcctgacc tactgagagg ccgcctccgt tcgtatactc gtggcggcgg
     7201 tcgcaaccga ccgctgggcg atggggctcg cccgaaccgc cggtccaggc tgatggctcc
     7261 tgcgacgagg aggtgtcggt cgtggcgacg gcgttgattg tgggggtggc gggcgcgatc
     7321 ggaacgctcg cgcggtacgg gctggacggt ctcgtcagcc ggaggttccc cggggccttc
     7381 cccctcggga cgttcgtcgt gaacatcacc ggagccctcc ttctcggcct cgtcttcacc
     7441 gtgctgacgg agcggttcat ggtgagcccc tcggtgcggt cggccatcac gatcgggctc
     7501 ctgggtgcct acacgacgtt ctccacgctc tcgctggaga cgctccgtct gatcgaggac
     7561 ggcgcctacc tcatcgcggc ggcgaacgcg ctcggaaccc tcgcggtcgg cctcgtcgcc
     7621 gtgtacgttg gcgtcgtgct ggggcgggcc gtctgatgag gatcgagggc gaggggaagc
     7681 tgctccggat cttcatcgga gaatcggata cctggcacgg taagcccctc tatcaggcca
     7741 tcgtggagcg ggccagggag gaggggctgg ccggggccac cgtgattcgg ggcatcgagg
     7801 ggttcggcgc gcactcccgc atccacacgg cccggatcct tcgtctgtcc gaggacctcc
     7861 cggtgatcat cgagatcgtc gattccgagg agcggatccg ggggatcctt cccgcgctgg
     7921 acgagatggt gactgacggc ctgctcacca tggagcgggt cgaggtgatc acgtatcggg
     7981 cgggagacga agccgagggc tgacgggctc accaggggct gatggggctc acccgagctc
     8041 gacgatcagc ggggcgtggt cgctcggctt cccctcgccg aaggtgggct tccgctcgtc
     8101 tcgctccacc cagacccgct cgcatcgctc ggcgatcggt cgcgtggcga gcgcgaggtc
     8161 gagccggagc ccccagcccc ggtggaacgc gccggcgcgg tagtcccaga acgtgaacgg
     8221 gccgggccca tcctcgtgga gccgcatgag gtccacgagg ccccacccca gcagttctcg
     8281 cacgcgctcc cgctccggct cgctggccag gttgcgccct cgccacttct cggggtcgtg
     8341 cacgtcgagg tccctgggcg cgacgttgaa gtccccgatc acgaggagag ggtcgctcgg
     8401 atcgtgctcc gtccggagcc atgaggagag ggcgtcgagc cacgtgagct tcagctcgta
     8461 ctggggggca cccacctcca ccccgttgac gacgtacacg cagatcactc ggatcccctc
     8521 caccgttgct gagacgaccc tggcctgctc gggcgcgggg tcgctgggga acccgggcgc
     8581 cacgtccttg agcggctccc gggagaggat ggccaccccg ttgcggccgg gctggccgtg
     8641 gaccgccgtg cggtagccga gcgcctcgaa cgcatcgccg gggaacgtgt cctcggccgt
     8701 cttcagctcc tggaggcaga gcacgtcggg ctcgtgtcgc cgcaggaggg cctcggtgcg
     8761 ggccagccgc atcctgatcg agttgacgtt ccagctgatg agcctcatct cccgcgctca
     8821 acctcccacg gccgtcacca ggaggagccc caggccgagc aggagcgcga tcgtgtgcac
     8881 gaccttctcc cgccagtcct cggtcaccgt gagctggggg atgaggtcgg tcgctccgat
     8941 gtacacgaag ttcccggccg cgaacggcac gaggaaggcg atctccaggg ccccggcggc
     9001 ccagtacgcg gccagggccc ccagggggaa ggtgagggcc gacaccaggt tggcgagtag
     9061 ggcccgccgg ggcccccacc cgctgtggac gaggacgccg aagtccccca gctcctgggg
     9121 aacctcgtgg gccgccgccg ccatccaggc gctgatcccc accggcgtcg ccagcacgaa
     9181 gctcgccccg acggccagcc ccccgatcag gttgtggagc ccgtcggcag ccaggatcag
     9241 ggtccccatg ggctggcgct cgcagtcggc cccccggtga cagtggtgcc agtgcaagaa
     9301 ctgctcgagc acgtagaagc tgacgaagcc accgatgaac cagaggtaga ccacgatggg
     9361 gtcgctcagc tcctcggccg cgctggggag catgtggaag agggcccctc cgatcagcgc
     9421 gccggcggcg aaccccacga gcggcagcag catcctgcgc agcgcgcgcc tggggagcac
     9481 gagggtaagg ctcccggcga gcgcgatcac gctcatggcg agcccgccga cgaggatcca
     9541 gccgagggtg gacatgccgg tacgctaacc ggtctcggtc ccgctccgcg gctcctcttc
     9601 ctcctcctgg ccccaccgct cgaaggagct gacgctcgcc gccaggacga ggccgtacac
     9661 gacgaaccct gtgagcccga tgatggccgc ggattgggcg gctccgccgg ggcatatccc
     9721 gagcaccgtg aggatcaggg tgatgaagga gatcgggaac gccacgacca cctccagcgc
     9781 ctgacgacgc ctgagcgccg agaagaacca caggatcagc gggacccaga gcgccatggg
     9841 aatcatgccg atggccctgc gctcggggca gaggaacgcc aggaacacga agagacccac
     9901 cgccgcggcg accgccgcgt acgcgaggac gtgcatcagg ttggtctgtc gtcgttgctg
     9961 cttcgtctcg gtcatgacag ctccttcagg acctccccca gcgccgaccc gaagcgctcg
    10021 tcgtcctccg gccgggccgt cgtcacccgc atgtacccgg ggccgagcct gggatcgtcg
    10081 aggggcttga tgaggatccc tcgctcctcg agcctctcgg ccacctcgtg cgcatcgtaa
    10141 ggggagaggt cggccaggaa gaagtaggcg ctggtgggga aggtccggat ccccatcccg
    10201 cgcagcgagg cggcgagatc cttcgcccag ccgcggaggt cctcgatccg ggccttgatc
    10261 tgctggtcgt gctggagggt ggccacggcc gccgcctgac ttggaagcgt cagcgggtag
    10321 gcgtcgttgt tggcgttgag gtcgtcggcc acctcctccg gcagcaccgc gtaccccacc
    10381 cggagcccgg ccaggctctg agccttggag aaggtcctcg tgaccagcag gttcgggtgc
    10441 ttcggcacca gccccgcgac cgacgggccg gcgaagtcga tgaaggcttc gtcgaccagg
    10501 aaccggacct cggggtgggc ccgcagcaga tccggcagcg gtcccatgtc gaatacggtc
    10561 cccgtggggt tgttggcgtt cacgatcacg accagcgtgg tcccctcggg gaccctcagg
    10621 ccggagaggt cgaaggcgaa gtcctcctcc ggccggagcc gggtctccgt ggatgagtga
    10681 gcgatctcgg ggaagagggg ataggtcggc gtgagcagat gcacgcgctc cccgaaccgg
    10741 tcgaacagct gtcgaaggat cagctccgag cccgcgttga tgtggagcag cggctcgggc
    10801 acgcggagcc gttcggcgag caggccgcgg aggggggcgg agtagggctc cgtgtagtag
    10861 ttggcctcgg ggagcgcctg ggtggccgcc gcgatcgcct cgggcagggg tgggagcggg
    10921 ttctcgcaca gcaggagctt gatcccgcgg aacgcccctc cccctttctc cgctcctgcc
    10981 ttctccgcgg agggggagcg aacggtcacg cttcggcttc cttcatcgct tggatcgcct
    11041 cggagatctc ggccagctcc tcctggagct ccgcttgccg ctcttcgagg tctgccaccc
    11101 gcttctcgcg tcccatgcgg cgccgcctcc gaacggggta tccggtctcc cacccgtacg
    11161 cgggcccgtg gcaccagggc ccatgtgcgt gccagcagca gctcatctcg tcgcctcctc
    11221 ggtccctcta tctccatggt gtgccttggg ggaaggtccg gacaggggtc gaaggtcccg
    11281 ggcacgcggg gcccttctcc gagcacctca ggaggtggcc cagaccgcgt agagcgaccc
    11341 gaggtagaag atgaggagga cgagcgcgtc cggctcgagc cggctgatcc tcgtctcctc
    11401 gccgtggacg atcgcggcga gcgcgaccgc catcaggccg atcgctccca cgcccgccgc
    11461 cacctggctg gctcccgccc ccgtgagcag cggcccgccc gtgtacgcgg cgtcggcgaa
    11521 cacgaggacg atcatgttga aggcgttgct gccgaagagg tttcccaccg cgaggtcgta
    11581 ggcgccgatc cggagagccc ccagcgaggc cacgagctcg gggagcgagg tggcgaccgc
    11641 gacgaagagg acgccgacga aggtggccga cagcccggag gtctcagcga tcccgtcgcc
    11701 cgcgatcgcg accacgggac cggccagcag gaccgcgagc gaggccagcg agaagcggac
    11761 gacggcggat cggagctcgc ctcgcggcga ctcggcctcc ccccactccg tgggggcggg
    11821 gtagtcggcg aaccgtccgt gtcgggagcg gcgcatccag gccatggcgg cgatccatgc
    11881 gaggaggatc aggatcgtgt cgatgccgac ccatccgagc atcgggcccc tgggcgtgac
    11941 cacgcccagg accgcgagcg acgtcagggc gatggccagg gtcccgacgc gcgcgtgccc
    12001 cagctcgacc gcaggccaga cgtgcttccg gtgcacgagg tcgatcacgg cgaggatcgc
    12061 catattgccc atgctgctgc cgaagatgct gcccacggcg aggtccacgg cctccgtcgc
    12121 cgccgcggct acgaccgtga cgatctcggg gagcgacgtg gcggccgcca tcagcagggt
    12181 ccccacgaac agaccgccca agcgggtgcg cgtggcgatc tcgtctcctg agcgagcgag
    12241 gacgctcccg gcccagacca gcaccaccgc tcccgccgcg aacaggagca gccactgcca
    12301 gaggctcatg gctcggggag cctactcggc cttcttcgag gtgctcttct tggccgccga
    12361 cttcttggcc gcggtcttct tggaggaggt cttcttcgcc gcgctcttcg ccccggccgt
    12421 cttcttcggc tcgcggcgct cgatctcgag gctccaacga gcggccacgc cggccatcgc
    12481 gcccacggcg gtgatcatcg gcgccgccac ggcccccacg atcccgaagg tcagcggcag
    12541 ctccatcacc gtctggccct ccgagttctt cacgacgata cggcggacgt tcccttcgtg
    12601 gaggagctcc tccaccttct cccggagggc gtcggcctgc accttcacct tctccgtcca
    12661 gaccttctcc tctgccatcg tggtctcctc tccatcgagt gcctacccat catgcctcgc
    12721 ccggcggccg gccgccttgt gcagggcatc tgccgccagc accgcggggc cggtcagaag
    12781 cgccaccccc cagccggcga gcgtcggggc cgcgtgccgc aggagctcgg ccacgggcgg
    12841 gatcagcaag aaccccagcg agagcagcag ctcgatcgcg gctcccacca caagcaggcg
    12901 gttcgtgagc cagcccagcg cccccggcca ccgggtcgcg ctgcggcagg caaaggcgtt
    12961 ggccgtctgc ccgatgacca ccgccatgaa cgtggccccg gaggccgcca gcaggggagc
    13021 ccccgttggg aaggggtcgc cgggccgcca gccccacgcc aggagcgaga cgacgaaggc
    13081 ggccatcccc atcaggccct cgaccggccc caggacgccg aaggcacgcc tggccacccc
    13141 tccgtccagg aggcgtcccc tggccggcag ctgctcgagg gtcctcggct ccgcgggctc
    13201 cgcgcccagc gctacggccg agagcgtgtc ggtgcccagg tcgagggcga ggatctgcag
    13261 gacgcccagg gcgaggggga agctcccgcc cgagagggcc cagaccaaga acggcgtgag
    13321 ctccgccaca ttgtccgtga gatggtaggt gaggaaccgc tgggcgttcg cgaagatggc
    13381 gcgcccctcc tccacggccg tgacgatcgt ggcgaagtcg tcgtccagga ggacgagatc
    13441 ggccgcctct cgggccacgt cggtccccga gcgacccatg gccaccccga tgtcggcctc
    13501 gtggagagcc ggcccgtcgt tgacgccgtc gccggtcatc gccacgatgt gcccccgctt
    13561 cctcagcgcg cgggcgatcc ggagcttgtc ctcgggatcc acgcggccga tcacgacgcc
    13621 gtcccggtcg acgagctcgc cgagggcccc gtcgtcgggc ggcaggtccg agcccacgag
    13681 cacgggagcg ccgggcgccc gcagccccgt ctcgtctgcg atcgtggccg cggtcgtcgg
    13741 gtggtcgccg gtaatcatcg ccacgtggat cccggcacgc cggcaggccg cgatcgcctc
    13801 ggcggcctcc ggacgcgggg ggtcctccag gccgatcagg gcgaggaggt tcagctcgcg
    13861 ctcggcttcc tctgccgtgg ccggcccggg accgcccgcg agatcccggg cggctacggc
    13921 gatcacgcgg aggcctcgct cgctcatccg ggccagggcg tggggggccc cggacgcggt
    13981 cctgcacagg ggcag
//
LOCUS       DASUTW010000334         3466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1020865, whole genome shotgun sequence.
ACCESSION   DASUTW010000334 DASUTW010000000
VERSION     DASUTW010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020865"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /gene="miaA"
                     /locus_tag="VF058_11545"
     CDS             complement(<1..570)
                     /gene="miaA"
                     /locus_tag="VF058_11545"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011981873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX6208975.1"
                     /translation="MADPSVPRALVGPTASGKTEASVELARRLDAEVILVDSMTVYRG
                     MDVGTAKPGPDERARVPHHLVDVVDPTEPFSVARFQALARQAVRGIVERGRVPLLVGG
                     SGLYFRAAVDDLEFPGTDPGLRDLLSREARLLGPERMHSRLADLDPAAAAKIDPDNAR
                     RSVRALEVAALTGRPFSSFAAAWDRYPKGR"
     gene            complement(642..1937)
                     /gene="miaB"
                     /locus_tag="VF058_11550"
                     /pseudo
     CDS             complement(642..1937)
                     /gene="miaB"
                     /locus_tag="VF058_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224300.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
     gene            complement(1948..>3466)
                     /gene="rny"
                     /locus_tag="VF058_11555"
     CDS             complement(1948..>3466)
                     /gene="rny"
                     /locus_tag="VF058_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX6208976.1"
                     /translation="AALVALVVGLILGFLLRAALRGRAVSAAESRARRLVLEAEEEAQ
                     TTVHRSLAEVKDEIAAMRREAEDEIRLRREEAARQEERQVRREEALESKAARIEQAEA
                     ALDRDRAALAAKADQLKAAGEDIQRRLERVASMTAQEAKEALAEGMREAAKREAMASI
                     REAEERAREEAEERARKIVTIAIQRVAAEQTADASVAVFPLPSDDLKGRIIGREGRNI
                     RHFEALTGVNLVIDDTPEAVVLSSFDPVRREVARLTLEKLVQDGRIHPARIEEMFERS
                     SGEVEEQIRRAGEQAVIDVGLTDVHPEMIRVLGRLQFRTSYGQNVLRHLVESAHIAGV
                     MAAELGIDPTLVKRAALMHDIGKAVTHEVEGSHALIGAELARRLKEPPEVVHAIESHH
                     GEVEQRTVIAVLAQTADQISGGRPGARRESIEQYVKRLARLEEICSAHPGVEKVYAMQ
                     AGREVRVMVEPEEVDDLAAQVLARDIAKQVEEELQYPGQIKITVVRETRASELAK"
BASE COUNT          445 a         1319 c         1174 g          528 t
ORIGIN      
        1 gcgtcccttc gggtaccggt cccacgccgc ggcgaacgag gagaagggtc gtcccgtcag
       61 ggcggccacc tccagggcgc gcacgctgcg ccgcgcgttg tcgggatcga tcttggccgc
      121 cgcggcaggg tcgaggtcgg ccagccggga gtgcatccgc tccggcccca gcagacgggc
      181 ctcccggctc aggaggtccc ggagcccggg atccgtgcca gggaactcga ggtcgtccac
      241 ggccgcccgg aagtagaggc cggaccctcc cacgagcagc ggcacgcggc ctcgctccac
      301 gatccctcgg accgcctggc gggccagggc ctggaaccgg gccacgctga agggctcggt
      361 cggatcgacc acgtccacga ggtggtgagg gacccgcgcg cgctcgtcgg gccccggctt
      421 ggccgtcccc acgtccatgc cgcggtagac cgtcatcgag tcgaccagga tcacctcggc
      481 gtccaggcgc cgggccagct cgacgctcgc ctcggtcttc cccgaggccg tgggcccgac
      541 gagcgcgcgc gggaccgatg ggtccgccac cggcgccgcc gctccccgcg tggcccgtgc
      601 cgtcctcatc gcgaggcggc caccgcggcg ggctctccgc tgaggtgatg cgggtgcccc
      661 cgcgtgatcc gcacgtcgag gaaggagccg ggctccgccc ggccctccca gttcacgacg
      721 cggttggtcc tcgtccttcc ctggaccccg cccttgcgac ccgagccctc cacgagcacc
      781 tcctggacgg agccctcgag gccgcggctc ttggccagcg agatcccctc ctgcagggcg
      841 accagccgat cgaaccgctc ctggactacc tccttgggac cctggggctc gtagccggcg
      901 gcctgcgtcc cggggcgcgg cgagtactgg aacgtgtagg ccgcgtcgaa acgagcctcc
      961 tgcaccacgc ggagggtctg ccggaagtcc tcctcggtct cgcccgggaa ccccacgatg
     1021 atgtccgtgc tgaccgcgat ccccggcacc gcctcgcgga tccgctccag ccacgagagg
     1081 tagcgggccg agcggtagga gcgtcgcatc gccttgagga tgcggtccga ccccgactgc
     1141 agggggaagt ggatgtgctc gcagaccttc tcgcactcgg ccatggcctc gatcacgtcg
     1201 ggcgtgaagt cgtggggatg ggggctcgtg aatcggatcc gctcgatccc ctccacctcg
     1261 ttcacggcgc gcagcagctc cgcgaagagc ggcttccgtg gggagtcggg gaccgtcagg
     1321 tcccggccgt aggtgttgac gttctggccc aggagggtga cctcgaggac cccctcggcg
     1381 gccagcccct ggacctcggc caggacctca cccagcggcc tggaccgctg gggcccccgg
     1441 acgagcggca cgatgcagaa cgagcacagg ttgtcgcagc ccgtggagac cgagacccag
     1501 gcggcgtgcg ccacctctct gcgggccgga agcgcgctgg ggaagacctc cgtatactcg
     1561 cggacgtcca tctggggacc ctcgcggtcc gcgcgctcca ggaggtcggc caggtacgga
     1621 acggcgtggg tacccacgac gacatcgacc cacggggctc gccgctggat ctcgcctcga
     1681 tccttctggg ccaggcagcc cgccaccacg atccgaagac gggggttggc ctccttcgcg
     1741 gcccggaggt ggccgaggtt cccgtagagt cggttgtcgg cgttctcgcg gaccgcacag
     1801 gtgttgagga cgacgacctc ggcctcgtcc acacgctcgg tccgctccag ccctcgggac
     1861 tccagaagcc ccgagatgcg ctcggagtcg tgctcgttca tctgacagcc gaacgtccgg
     1921 atcaggtacc gctccatggc ccccaggcta cttcgccagc tcgctggcgc gggtctcccg
     1981 cacgaccgtg atcttgatct ggcccgggta ctgcagctcc tcctccacct gcttggcgat
     2041 gtccctggcc agaacctgcg cggcgaggtc gtccacctcc tcgggctcga ccatgacccg
     2101 gacctcgcgg ccggcctgca tcgcgtagac cttctccacg ccggggtgcg ccgagcagat
     2161 ctcctcgagg cgcgccagcc gcttgacgta ctgctcgatg ctctcgcgcc gggcgcccgg
     2221 ccggccgccg gagatctggt cggccgtctg ggccagaacc gcgatgacgg tgcgctgctc
     2281 cacctcgccg tggtgggact cgatggcgtg gacgacctcg ggcggttcct tgagccggcg
     2341 ggcgagctcg gctccgatca aggcgtgcga gccctccacc tcgtgggtaa ccgccttccc
     2401 gatgtcgtgc atcagcgcgg cccgcttgac gagcgtgggg tcgatcccca gctcggcggc
     2461 catcaccccg gcgatgtgag ccgactccac caggtggcgg agcacgttct ggccgtagga
     2521 ggtcctgaac tggagccgcc ccaggacccg gatcatctcg gggtgcacgt cggtcagccc
     2581 cacgtcgatt acggcctgct ccccggccct ccggatctgc tcctcgacct ccccggagga
     2641 ccgctcgaac atctcctcga tcctggccgg atggatgcgc ccgtcctgga ccagcttctc
     2701 gagggtgagg cgggccacct cccgacgcac gggatcgaag ctcgagagca cgaccgcctc
     2761 gggcgtgtcg tcgatcacca ggttcacgcc cgtgagggcc tcgaagtggc ggatgttccg
     2821 cccctcccgg ccgatgatgc ggcccttgag gtcgtcggac gggagcggga agacggcgac
     2881 cgacgcgtcc gccgtctgct ccgcggccac ccgctggatc gcgatcgtga cgatcttccg
     2941 ggctctctcc tcggcctcct cgcgggcccg ctcctccgcc tcccggatgc tggccatggc
     3001 ctctcgcttg gcggcctccc gcatcccctc cgccaaggcc tccttggcct cctgcgcggt
     3061 catcgaggcc acccgctcca ggcgcctctg gatgtcctcg ccggcggcct tcagctggtc
     3121 ggcctttgcc gccagcgcgg cccggtcccg atccagggcg gcctcggcct gctcgatccg
     3181 ggcggccttg ctctccaggg cctcctcgcg acggacctgc cgctcctcct gcctcgcggc
     3241 ttcctcccgc cggagccgga tctcgtcctc ggcctccctc cgcatcgccg cgatctcgtc
     3301 cttgacctcg gcgagcgagc ggtgaaccgt cgtctgggcc tcctcctccg cctcgagcac
     3361 gagccgtcgg gcgcggctct cggcggcgga gaccgcacgg cctcgcagcg cggcgcggag
     3421 gaggaacccg aggatcaggc ccacgacgag cgcgacgagg gctgca
//
LOCUS       DASUTW010000335         3132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1540211, whole genome shotgun sequence.
ACCESSION   DASUTW010000335 DASUTW010000000
VERSION     DASUTW010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540211"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VF058_11560"
     CDS             complement(<1..585)
                     /locus_tag="VF058_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6208977.1"
                     /translation="MGQRVLITGIAGHLAGELARRLEDDPEVEYLVGVDLVEPRADLT
                     RLEYVRADIRNPLVVKVLQTTEVDTVVHLSLLSTPHGVGGRSNMKEINVIGAMQLFAA
                     CQRAEAVRRIVMKSTTAVYGSGPKDPAVFTEDMSARSEAPHGYAKDAIEVEAYARSFA
                     RRRDDVDLTVLRFANFIGPGVASTLTRYFSLPLIP"
     gene            complement(587..1405)
                     /locus_tag="VF058_11565"
     CDS             complement(587..1405)
                     /locus_tag="VF058_11565"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX6208978.1"
                     /translation="MTRPILFLSDYGLADEFVGVCHAVMVRIAPGSPIIDLAHEIPPQ
                     DVHRGAAVLRQAARHGPRNGVYVAIVDPGVGTERRPVAIQAGDAFLVGPDNGVLSTAW
                     TELGGARRAFKISSREVMLDKVSPTFHGRDVFSPCAAHLSNGMSIEEVGEEVDPATLV
                     EVALPGARIEPGYVHCKVIGIDRFGNVQLSARPDDLSEAGIPSDDLIHIRAGGRGYDV
                     AVRETFAHVEEGEGVVVVDSAGFLTLAKNRRNAARQLGISVGDHVVLAPVTPGA"
     gene            1453..1755
                     /locus_tag="VF058_11570"
     CDS             1453..1755
                     /locus_tag="VF058_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208979.1"
                     /translation="MGTSADLEFSDWAKDILERSQEAARRFNPRARIRLVLEDGAMEA
                     RLTEEPETGDREIDAGGVTVLVAPGIEGLVDVEEPHDRIVLRPAGSTPNPRGHHES"
     gene            complement(1743..2426)
                     /locus_tag="VF058_11575"
     CDS             complement(1743..2426)
                     /locus_tag="VF058_11575"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6208980.1"
                     /translation="MRRSALLLLVLLAACGRQAAPQPGEVIRFETSDGVELAGELRGE
                     GTSGVVLGHMYGSSREAWAAFAQQAADAGYRTLAFDLRGFGESGGTSSAPDAPTDLRA
                     AVEAIREHGAQEVVVIGASFSGTGALVAAGEENWAGVVTLSAPASFEGLRADEAVVEA
                     VDEPKLFIAAQGDGSAASSAQGFYDASSGAKRVEIVVGDEHGTDLLESSRAQLVRDLL
                     MAFLRAQLS"
     gene            complement(2428..>3132)
                     /locus_tag="VF058_11580"
     CDS             complement(2428..>3132)
                     /locus_tag="VF058_11580"
                     /inference="COORDINATES: protein motif:HMM:NF027889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp1/GlmU family protein"
                     /protein_id="HEX6208981.1"
                     /translation="AVGLRICRQLSDLEHDSLARADVLAFVGSTSPQGHLLPLVVGTR
                     LLLDRALAEGCDLVVIDTTGLVSGVYGQLLKGYKVQLLQPDHVIGIERGSELAPVLGV
                     ARRFSAAQVESLKVHPDVVPTSVEQRALNRQESLKRTFGGPLQRWRVKPTVFLPTLPD
                     LFDLAALDRIVVGLSDGKGGTIGIGYLEHVAEDGGLRLVSPATEGPKALVLGSVRLEE
                     DFRSRRVELRNLLGSD"
BASE COUNT          485 a         1132 c         1107 g          408 t
ORIGIN      
        1 ggggatcaac gggagggaga agtagcgcgt gagggtcgag gccaccccgg gcccgatgaa
       61 gttggcgaag cgaaggacgg tgaggtccac gtcgtcccgg cgacgagcga agctccgcgc
      121 gtaggcctcg acctcgatcg catctttcgc gtagccatgt ggggcctcgg agcgcgcgct
      181 catgtcctcg gtgaacaccg cgggatcctt cgggccggag ccgtagacgg ccgtcgtcga
      241 cttcatgacg atccggcgca cggcctccgc ccgctggcag gccgcgaaga gctgcatggc
      301 cccgatgacg ttgatctcct tcatgttgct gcggccgccg acgccgtggg gcgtggagag
      361 caggctgagg tgcacgacgg tgtcgacctc ggtggtctgg agcaccttca ccacgagggg
      421 gttccggatg tcggcccgca cgtactccag cctggtgagg tccgcgcgcg gctccacgag
      481 gtccacgccg acaaggtact cgacctcggg gtcgtcctcg agccgccgag ccagctcccc
      541 cgcgaggtgc cccgcgatcc cggtgatcag gacgcgctgg cccacgtcag gcccccgggg
      601 tcacgggcgc caggacgacg tgatcgccca cgctgatccc gagctgcctc gccgcgttgc
      661 gacggttctt ggccagcgtg aggaaccccg cggagtccac gaccacgacc ccctcaccct
      721 cctcgacgtg ggcgaacgtc tcccgcacgg ccacgtcata cccgcgaccg ccggctcgta
      781 tgtggatcag gtcgtcggag gggatgccgg cctccgacag gtcatcgggg cgcgcggaca
      841 gctggacgtt gccgaagcga tcgatgccga tcaccttgca gtggacgtag cccggctcga
      901 tccgagcccc cggaagggca acctcgacca gggtcgcggg gtcgacctcc tcgccgacct
      961 cctcgatcga catgccgttc gagaggtgcg ccgcgcacgg ggagaacacg tctcgcccgt
     1021 ggaacgtggg cgagaccttg tcgagcatca cctcccgcga ggagatcttg aacgcccgac
     1081 gagcgccgcc gagctcggtc cacgcggtcg agaggacgcc gttgtcgggg ccgacgagga
     1141 acgcatcccc tgcctggatc gccaccggcc gccgctcggt gccgacgccg gggtccacga
     1201 tcgccacgta gacgccgttg cgcggcccgt gtcgggcggc ctgccggagg accgcggcgc
     1261 cccggtgcac gtcctgaggc gggatctcgt gggccaggtc gatgatcggg ctgccgggcg
     1321 cgatccggac catcaccgcg tgacagacgc cgacgaactc gtcggccagc ccgtaatccg
     1381 agaggaacag gatcggcctc gtcatgaacg accggaaggt accagagccg gggtgatagc
     1441 gtggaccgcc ggatgggaac gagcgcggat ctcgagttca gcgactgggc caaggacatc
     1501 cttgagaggt cccaggaggc cgcgcgccgg ttcaacccgc gagcacgcat ccggctggtg
     1561 ctcgaggacg gggcgatgga ggcccggctc accgaggagc ccgaaaccgg ggaccgcgag
     1621 atcgacgccg gcggcgtcac cgtgctcgtc gcccccggga tcgaggggct cgtggacgtc
     1681 gaggagccgc acgaccggat cgtactccgg ccggccggct cgacccccaa cccgcgcggc
     1741 catcacgaga gctgagccct gaggaaggcc atcaggagat cccggacgag ctgggccctc
     1801 gagctctcca gcaggtcggt gccgtgctcg tcccctacca cgatctccac gcgcttggcc
     1861 cccgaggacg cgtcgtagaa tccctgagcc gagctcgccg cggagccgtc gccctgcgcg
     1921 gctatgaaga gcttcggctc gtccaccgcc tcgaccacgg cctcgtcggc ccgcagcccc
     1981 tcgaacgagg ccggcgccga cagcgtgacc acgccggccc aattctcctc gcccgccgcc
     2041 accagcgcgc ccgtccccga gaagctggcg ccgatcacca ccacctcttg cgctccgtgc
     2101 tcgcggatcg cctcgaccgc cgcccggagg tccgtcggcg cgtcgggggc gctgctggtc
     2161 ccgccggact cgccgaagcc ccggaggtcg aaggcgagag tccggtagcc ggcgtcggcc
     2221 gcctgctggg cgaaggcggc ccacgcctcg cggctcgagc cgtacatgtg gccgaggacg
     2281 accccgctcg tcccctcccc gcgcaactcg cccgcgagct cgacgccgtc cgacgtctcg
     2341 aaccggatca cctcgcccgg ctggggcgcc gcctggcgcc cacaggcggc gaggaggacc
     2401 agcaggagca gggccgaccg gcgcatgtca gtccgagccg aggaggttcc gcagctccac
     2461 ccgccggctg cggaagtcct cctcgagacg gaccgagccg agcacgagcg ccttcggtcc
     2521 ttcggtggcg ggcgagacga gccgcagacc cccgtcctcg gccacgtgct ccaggtagcc
     2581 gatcccgatc gtccctccct tcccgtccga cagcccgacg acgatgcggt cgagcgcggc
     2641 gaggtcgaag aggtcgggga gggtggggag gaagacggtc ggcttgacgc gccaccgttg
     2701 gagcggcccg ccgaaggtgc gcttcagcga ctcctggcga ttgagggctc gctgctcgac
     2761 cgacgtcggg accacgtcgg ggtggacctt caaggactcg acctgggcgg cgctgaagcg
     2821 gcgcgccacg cccagcacgg gggccagctc gctcccccgc tcgatcccga tcacgtggtc
     2881 cggctggaga agctgcacct tatagccctt gaggagctgg ccgtacacgc ccgagacgag
     2941 cccggtcgtg tcgatcacga cgaggtcgca gccctcggcg agcgcgcggt cgaggagcaa
     3001 ccgcgtgccc acgaccagcg ggaggaggtg gccctgggga gacgtcgacc cgacgaacgc
     3061 gaggacgtcg gcccgggcca gcgagtcgtg ctcgagatcg gagagctggc ggcagatccg
     3121 caggcccacc gc
//
LOCUS       DASUTW010000336         3642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1150526, whole genome shotgun sequence.
ACCESSION   DASUTW010000336 DASUTW010000000
VERSION     DASUTW010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150526"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..817
                     /gene="whiG"
                     /locus_tag="VF058_11585"
     CDS             29..817
                     /gene="whiG"
                     /locus_tag="VF058_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008745397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HEX6208982.1"
                     /translation="MASDQQAVEQDELTALWKEYKANGSQEIRERLILHYAPLVKFVA
                     GRLAAGLPQNVDQADLTSFGMFGLIDALEKFDLSRAVKFETYAIPRIKGAIIDELRAL
                     DWVPRSVRFKARELDKAYQTLEAKLGRSPSDEEVAEHLGIGLDDLHEVVSQISFVSVL
                     ALDDVVAGGEDRSESRTLLDTLADAANADPTAELEGQEMRGLLSHAINSLAEREKVVV
                     TLYYFEGLTLAEIGEILGVTESRICQIHTKAVGNLRRGLDDRDA"
     gene            880..1416
                     /locus_tag="VF058_11590"
     CDS             880..1416
                     /locus_tag="VF058_11590"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX6208983.1"
                     /translation="MHGSSVVAAATGILTAIVGSVPGPSVGEAVAVGGYAWPVLGRVL
                     RGFEPAATPYGPGHRGIDIGAERGTRVRAAERGVVSFAGRVAGQRFISILHPGGIRTT
                     YSWLSRIDVEEGQTVLRGALIGRTGDGHPGSEAPHLHLGARIDDVYLDPLTLLEPVSL
                     VGIVRLAPLGGAWARSPP"
     gene            1428..2723
                     /locus_tag="VF058_11595"
     CDS             1428..2723
                     /locus_tag="VF058_11595"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX6208984.1"
                     /translation="MRLRAPLVLGLLLSAAWIPLPASAQRAPWPVAGRTPAHAAVTEG
                     PAPPYRVVWETELEDGPPAAAPVLGEAMLVVLGRSEVAALDPESGDELWSVERAEGTG
                     GAPAVAGDLVLFVEGDGEEAALVAVGAPDGEEEWRTEIEGHAPGGVVVAEGRAYVASR
                     RGTVYAVDLDGGSVAWTFPENPAGRRFESAPAVAGDLLLVTGQITGERTSFLQALDRE
                     TGEPAWGFRPEGIGLGISSPSEEGGLVLAGTVDGRPRASAFDLAGGGSEWTTDIRFPF
                     AGRQMPIVADSDVVIADAAGHLYRLDRSDGELRWTYRVPGFLIGGSPSIAGGSVVVGD
                     LSGQVSAIDLESGLLVWRRSFGDDQVHPIASDGDRLYVAARGGTVAALEHDPDGSLLE
                     EPSPTTLFLDRALLNFGAAFAIVGGALALLSLLWRRRGG"
     gene            2723..>3642
                     /locus_tag="VF058_11600"
     CDS             2723..>3642
                     /locus_tag="VF058_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208985.1"
                     /translation="MGRKGKRRKRPRATDRASQRPEELVESTGPAPDEETPAPDRVAT
                     EEEPSDAPPRRGLFAGFSIPSPHPPLLETLGRGVPPASDPRVLAVAFLFVLALWGLVV
                     GLGSEAPPALMAGFWAVPPISVFFDANVVSTIVPGAVASLGLLVVVLALRSLSLTGII
                     RWIAGEQGDPARTEERRGFLATSLSMFAIHSAELGLVLIVFTLAQGLLGGQIGALLAW
                     GLGAYFLGFVPAVIAVEGASAAEGLRRGLRAARLPGARHLTFVMLYLLVTLWMSQFAP
                     GGILPPATPSIGIWAYVLVATFVHLAAIGAL"
BASE COUNT          444 a         1290 c         1353 g          555 t
ORIGIN      
        1 cccgcgagcc tgacgaggag gtcgaccgat cgccagcgac cagcaggccg tcgagcagga
       61 cgagctgacc gcgctctgga aggagtacaa ggcgaacgga tcccaggaga tccgggagcg
      121 cctcatcctc cactacgccc ccctggtgaa gttcgtggcg ggacggctcg ccgccggcct
      181 cccccagaac gtggaccaag ccgacctcac gtccttcggc atgttcgggc tcatcgacgc
      241 cctggagaag ttcgatctgt cccgcgccgt gaagttcgag acgtacgcga tcccccgcat
      301 caagggcgcg atcatcgacg agctgcgggc cctggactgg gttccccggt cggtgcggtt
      361 caaggcccgg gagctggaca aggcttacca gaccctggag gcgaagctgg gccgatcgcc
      421 atcggacgag gaggtggccg agcacctcgg catcggcctg gatgatctgc acgaggtggt
      481 gagccagatc tcgttcgtgt ccgtgctcgc cctcgacgac gtcgtggccg ggggagagga
      541 ccgcagcgag tcgaggacgc tgctcgacac gctggccgac gcggcgaacg ccgaccccac
      601 ggcggagctc gagggccagg agatgcgggg ccttctctcc cacgccatca actcgctggc
      661 ggagcgggag aaggtcgtcg tcacgctcta ctacttcgag gggctgacgc tggccgagat
      721 cggggagatc ctgggcgtga ccgagtcccg gatctgccag atccacacga aggcggtggg
      781 aaacctccgg cgcgggctgg acgaccgcga cgcctgatcg ccgttcgtgt cggaccctcc
      841 tgctaaggtc gtcccgtccc cctcggagga ggtggacgca tgcatggatc gtccgtggtc
      901 gcggccgcga cggggatcct cacggcgatc gtcgggtccg tccccggccc ctcggtgggc
      961 gaggcggtcg cggtgggggg ctacgcctgg ccggttctgg gccgggtcct gcgaggcttc
     1021 gagccggcgg cgaccccgta cggaccgggc catcgaggga tcgacatcgg agccgagcgc
     1081 gggacccggg tgcgggccgc cgagcggggc gtggtgtcgt tcgccggacg ggtcgccggc
     1141 cagcgcttca tctcgatcct ccatccgggc gggatccgga ccacctactc gtggctgtcc
     1201 cgcatcgacg tggaggaagg gcagaccgtc ctgcgcgggg ccctgatcgg gcggaccggc
     1261 gacggtcacc cggggtccga ggcgccccac ctccatctcg gcgcccggat cgacgacgtc
     1321 tacctggatc ccctgacgct cctggagccg gtgagcctgg tggggatcgt gcggctcgcc
     1381 ccgctgggag gggcgtgggc tcgctcgccc ccatagaatg ggcgatcgtg cgtttgcgag
     1441 cccccctcgt cctcggcctg ctgctctcgg cggcctggat ccctctgccc gcgagcgccc
     1501 agcgagcccc gtggccggtg gcaggccgga cacccgccca cgcggccgtg acggagggcc
     1561 cggcgccccc gtaccgggtg gtctgggaga cggagctcga ggacggccct ccggccgccg
     1621 ccccggtgct cggcgaggcg atgctcgtcg tcctgggccg atctgaggtc gcggcgctcg
     1681 atcccgagag cggcgacgag ctgtggtctg tggagcgtgc cgagggcacc gggggcgccc
     1741 ctgccgtggc gggcgacctc gtcctcttcg tggaggggga tggggaggag gctgctctgg
     1801 tcgccgtggg cgccccggac ggcgaggagg agtggcgaac cgagatcgag ggccacgccc
     1861 cgggcggcgt cgtcgtggcc gaggggcggg cgtacgtcgc ctcgcgtcgc gggaccgtgt
     1921 acgcggtgga cctcgacggc ggctcggtcg cgtggacgtt ccccgagaac cccgccggac
     1981 ggcggttcga gagcgccccg gcggtggccg gcgacctcct cctcgtcacc gggcagatca
     2041 cgggcgagcg gacctcgttc ctccaggccc tcgatcggga gaccggcgag cccgcctggg
     2101 gctttcgccc cgaggggatc ggcctcggca tctcctcgcc ctccgaggaa ggcggcctgg
     2161 tgctggcggg gaccgtggac gggcggcctc gagccagcgc cttcgatctg gcgggcggcg
     2221 ggagcgagtg gaccacggac atccgcttcc ccttcgcggg gcgtcagatg ccgatcgtgg
     2281 ccgactccga cgtggtcatc gccgacgccg cgggacacct ctaccggctg gatcggtcgg
     2341 acggcgagct ccggtggacc taccgagtcc cggggttcct catcggcggg tccccctcga
     2401 tcgcgggggg ctcggtggtc gtgggtgacc tcagcggcca ggtctcggcg atcgacctgg
     2461 agagcgggct cctcgtctgg aggcggtcct tcggcgacga tcaggtccat cccatcgcct
     2521 cggacggaga ccgcctgtac gtggccgccc gaggagggac ggtggccgcc ctcgagcacg
     2581 acccggacgg gagcctcctc gaggagccct cgcccacgac cctgttcctg gacagggcgc
     2641 tgctgaactt cggcgcggcc ttcgccatcg tggggggggc gctcgcgctg ctgagcttgc
     2701 tgtggaggag gagggggggc tgatgggccg caagggcaag cgacggaagc ggcctcgcgc
     2761 gacggatcga gcttcccagc ggcccgagga gctggtggag tcgaccggcc ccgcgccgga
     2821 cgaagagacc cccgctcccg accgggtggc gactgaggaa gagccctccg acgctccccc
     2881 gcggcgtggg ctcttcgccg ggttctcgat cccgtccccc cacccgcccc tcctcgagac
     2941 cctcggacgc ggcgtgcctc ccgcctccga cccccgcgtc ctggccgtgg ccttcctctt
     3001 cgtgctcgcg ctctggggcc tcgtggtggg cctggggagc gaggcacccc cggcgctgat
     3061 ggcggggttc tgggcggtcc cgccgatctc ggtcttcttc gacgcgaacg tggtctcgac
     3121 gatcgtgccg ggggcggtgg cctccctggg actcctcgtc gtcgtgctcg ccctccggtc
     3181 gctctcgctg acagggatca tccgctggat cgccggagag cagggggacc ccgctcggac
     3241 ggaggagcgg cgggggttcc tcgccacttc gctgtccatg ttcgcgatcc actccgccga
     3301 gctcggcctc gtgctgatcg tcttcacgct ggcccagggg ctcctgggcg gccagatcgg
     3361 cgccctcctc gcctggggcc tcggcgccta cttcctgggc ttcgtccccg ctgtgatcgc
     3421 cgtggaggga gcctcggcgg ccgagggcct ccgccggggt ctccgcgccg cgaggctccc
     3481 aggcgcccgc cacctcacgt tcgtcatgct ctacctgctg gtgaccctct ggatgagcca
     3541 gttcgcgccc ggggggatcc tcccgccggc caccccctcg atcgggatat gggcctacgt
     3601 cctcgttgcg acgttcgtgc atctggccgc gatcggcgcg ct
//
LOCUS       DASUTW010000337         7464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1928716, whole genome shotgun sequence.
ACCESSION   DASUTW010000337 DASUTW010000000
VERSION     DASUTW010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7464
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1928716"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="VF058_11605"
     CDS             <1..1108
                     /locus_tag="VF058_11605"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX6208986.1"
                     /translation="LVFVVIIAQLAHRLAEHPSWSGVGWFVFLFYAVWSSWINGTLYH
                     DLHTTNDVSVRVFTFAQMLAVAVMAVYAADVPGDGSTGFALGYAANTLLLVILWARTG
                     LHDPSHRPASVPYSTAYLISAVLFAASVGVEVPVRYWLWVLGLLAEVGGIVVAFHRWT
                     PPASQGGDAVIAATPSLIERLGLFVIIVLGEVIVGAVTGMAEISPLDLGEIVIGLLGV
                     LVAIGLWWIYFDLVSHRGPVSRRTQLWLNLHLPLVIAMAAGGAGVLNTVEHAGEPLPD
                     AVRWLLVGSLAVALLSVAALTWTLEARRAQPRLYRTAEAAILLGGMLALAVGATEWGA
                     KASLTAMVIVLLIPVAAGSIVWRRQTEPDPFGLA"
     gene            1174..2424
                     /locus_tag="VF058_11610"
     CDS             1174..2424
                     /locus_tag="VF058_11610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX6208987.1"
                     /translation="MIRSLDELADRTPATRDRVMDFLRAFSIVTVVLGHWLIAIIWWE
                     DGLLYQTSAIGETSGLWLATWFLQVMPIFFFVGGFANMITYDAYKRRGDPTWAFVKSR
                     VERLLRPSLVFLGLWLAVQVALHLADVGRPTGPVLWGDTRLLRGMNPPPGTLPFGPLW
                     FLAVYLVVVAISPATIWLHRRFRWWIVGVLAAGPFVVDLFGFGFDMYRLRYLNIPFVL
                     LLPHQLGHFYADGTLQRLPRKVFWAMAIGGLAGLLLATNPWLFDWLGGDRRFEWFPMI
                     GYYPKSALGTDAEPISNAFPPTIVFLLVGIWTIGAVMLLRDRLAPWLERRGPWKATIL
                     GNSVIMTLFLWHMTAYLLAILLLWPMGFGREQDSTLRWWLERPLWILVPGLILLAIVG
                     LVGRFERGSVRTRAAPPEERLRTA"
     gene            complement(2411..2899)
                     /locus_tag="VF058_11615"
     CDS             complement(2411..2899)
                     /locus_tag="VF058_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208988.1"
                     /translation="MYRWWVFLHVVGGFGFFMAHGASAMASIRLRTERSPERVKALLD
                     LSSSSLGFMYVSLLLLLAGGIVSGFLGDWWGEGWIWTAIALLILIMGAMYGLASGYYN
                     QVREAAGIQTYEQRKKGIEAGPPDPGRLDVLLSSSRPMVIMVVGFGGLLLILWLMILK
                     PF"
     gene            2945..4972
                     /locus_tag="VF058_11620"
     CDS             2945..4972
                     /locus_tag="VF058_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208989.1"
                     /translation="MAPRLKHRLDDVVRALVTPRAEPGALPLVDAAAGFRPGDRSAVS
                     SSSAFLLALAGSDDAAVHLSAAAEGGDAVAAFLADGLRRIDEELDGLGDRDAALAERM
                     GALAAFLEREDAGSSVDELAERIWAVFFPEGVGIRGHEEARAAELRARRAIDITERGP
                     AVTDAARQVLFTVNLLLTGPPAGVEVDDLDLPSEITGALRDPGPQAFWYDHPVQIGID
                     APSSELRYGLAAMDRGMAFERERGADRPLRVVVSLSTTHERLQEIARPYLEHEVREMG
                     GLEHLELYAFGEADAERLVAKVLAPAAEAFLDRPHARELLGVLGVRGPYGRHHSFGKA
                     IAAVWHVLIDPEVVATFKTDLDHSWPQDELVSETGASLLEHFAFPLWGGTGIDAEGRR
                     VELGGFAAALVNEADIDQGLFTTDVPVPDRSPEANELLFWSALPQGLSTLAEASTRYG
                     IDGPDGVTTAIERVHVHAGTVGILVEALRRHRPFTPSFIGRAEDQAYILSTLWGSEPR
                     LAHVHHRELIQRHDKKAFADAAIEAARVGTWVGDHERLVLFSELARHLGPGRDEVKTV
                     AGPFTGAFISQIPATVTLLRLTLKAGVLAAKGAGDEAEELLAMAAERLGRTLAFVEGG
                     ELAERLDAERRGWDLLYETLGELERRLAAGDAEAERLADRARRVVAACAVG"
     gene            complement(4980..5615)
                     /locus_tag="VF058_11625"
     CDS             complement(4980..5615)
                     /locus_tag="VF058_11625"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208990.1"
                     /translation="MTAAFAVLASMTMPAAQAAPMCLGKRATIVGTSKGEVIRGTKRN
                     DVIVAKGGDDVIRGKGGHDRICAGGGDDVLIGGKGRDRLDGGFGADMFRGGPGRDTVS
                     YVGRTAKVDVVLDDVANDGYITGEGDNVRTDVENAIGGDGPDGLVGSALVNVFHGGPG
                     NDDLFGGDGNDRLFGEGGDDFILGQNGDDDRANGGPGTDDCDAETEIACEV"
     gene            5889..6755
                     /locus_tag="VF058_11630"
     CDS             5889..6755
                     /locus_tag="VF058_11630"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6208991.1"
                     /translation="MFGITRENLRHLATTLRLGPRSVPAVYESLGTDFFLALAPGWLN
                     LGWWEGEGTEEEAPRAPVRLVEKLAERLPPGGVTLDVANGLGAQDPVIARVVRPRFLA
                     AVNVTELQLRAGRGRLDEARARPVLADAVRLPFGEDAFDGLISVEAAFHFPSRAAFFR
                     EAVRVLRPGGVLSMSDLTAERWPRGVRDAAAGLAQLRVWGLRVRPLASGGRVAALAEE
                     AGLEDVRVELCASRTIDPAMRVMRGRLAHPRGASRAQVWASRVALGQIERLREGGYVQ
                     YALLTARAPDRA"
     gene            6852..7427
                     /locus_tag="VF058_11635"
     CDS             6852..7427
                     /locus_tag="VF058_11635"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX6208992.1"
                     /translation="MGASTVRKVAAITSLVVAGVVALLLALPGAEAAGKKCFGKTPTI
                     KSNATVIKGTNGNDVILAGKGDNQIYGRKGFDLICAGGGQDFVDGGPGRSKIKGGAGK
                     DAFQGGGKADVFYGGGGDDTSDPGGGPDVIYGGPGNDGSNNDLGGDAGNDRIYGQDGS
                     DNIDGGDGDDFCDGGAGDDNASDCETEVSIP"
BASE COUNT         1000 a         2583 c         2709 g         1172 t
ORIGIN      
        1 cctcgtcttc gtggtcatca tcgcgcagct ggcgcaccga ctcgccgagc atccctcgtg
       61 gtcgggcgtc ggttggttcg tcttcctctt ctacgcggtg tggtcctcgt ggatcaacgg
      121 gaccctgtac cacgacctcc atacgaccaa cgacgtcagc gtgcgggtct tcaccttcgc
      181 ccagatgctg gccgttgccg tcatggccgt gtacgcggcc gacgtccccg gcgacggctc
      241 gaccgggttc gcgctcggct acgcggccaa cacgctcctg ctggtgatct tgtgggcccg
      301 taccgggctc cacgacccct ctcaccggcc ggcgtcggtc ccgtactcca ccgcctatct
      361 catctcggcg gtcctgttcg cagcgagcgt cggggtcgag gtcccggtgc gctactggct
      421 gtgggtgctg ggactgctcg ccgaggtggg cggcatcgtc gtcgccttcc accgctggac
      481 gccgccggcg agccaggggg gtgatgcggt catcgcggcc accccctcgc tgatcgagcg
      541 gctcggcctg ttcgtcatca tcgtcctggg cgaggtcatc gtcggggccg tcacaggcat
      601 ggccgagatc agcccgctcg atctggggga gatcgtgatc ggtctccttg gggtgctggt
      661 ggccatcggc ttgtggtgga tctacttcga tctcgtctcg catcgggggc ctgtctcgag
      721 gaggacgcag ctctggctca atctccacct ccccctcgtg atcgcgatgg cggccggggg
      781 cgccggcgtg ctgaacacgg tcgagcacgc gggagagccg ctgcccgacg ccgtgcgatg
      841 gctgctcgtc ggctcgctcg cggtcgcgct cctctcggtc gcggccctga cgtggacgct
      901 ggaggcgcgg cgggctcagc caaggctgta ccgaacggca gaggcagcca tactgctcgg
      961 tggcatgctc gcgctggccg tcggcgcaac cgaatgggga gcgaaggcca gtctgaccgc
     1021 catggtgatc gtcctcctga tcccggtggc ggcgggttcg atcgtgtggc gcaggcagac
     1081 cgagccggat cccttcggcc tcgcctgaga cgggctctac atcggcgtcc acgagcgaga
     1141 accccccaac ggggagcgat agtctgagca gtcatgatcc gctcgctgga cgagctcgcc
     1201 gatcggaccc ccgccacccg cgaccgagtc atggacttcc tgcgtgcgtt cagcatcgtc
     1261 acggtcgtgc tcggccactg gctgatcgcg atcatctggt gggaggatgg cctcctctac
     1321 cagacgagcg cgatcgggga gacctcgggg ctctggctcg cgacgtggtt cctgcaggtg
     1381 atgccgatct tcttcttcgt cggtgggttc gccaacatga tcacctacga cgcgtacaag
     1441 cggcggggag atcccacctg ggccttcgtc aagagccgtg tggagcggct gctccgtcca
     1501 tcgctcgtct tcctcgggct ctggctcgcg gtccaggtgg cgctgcacct ggccgacgtg
     1561 ggtcggccca ccggcccggt gctctggggc gacacgcggc tgcttcgggg gatgaacccg
     1621 ccgcccggaa ccctcccgtt cggaccgctg tggttcctgg ccgtgtacct cgtcgtcgtg
     1681 gcgatcagtc cggcgacgat ctggctccac cgccgcttcc gctggtggat cgtaggggtt
     1741 ctggcggcgg ggccgttcgt ggtggacctc ttcgggttcg gcttcgacat gtatcggctg
     1801 cggtacctca acatcccgtt cgtgctgctt ctgccccacc agctcgggca cttctacgca
     1861 gacgggaccc tgcagcgcct gccgaggaag gtcttctggg cgatggcgat cggcgggctg
     1921 gcggggctcc tgctggccac gaacccgtgg ctcttcgatt ggctgggagg agaccggcgg
     1981 ttcgagtggt tcccgatgat cggctactac ccgaagagcg cgctgggcac cgacgccgag
     2041 ccgatctcga acgcgttccc gcccacgatc gtcttcctcc tcgtggggat ctggacgatc
     2101 ggggcggtga tgctgctgcg cgaccggctg gccccgtggc tggagcggcg cggcccgtgg
     2161 aaggcgacga tcctcggcaa cagcgtcatc atgacgctct tcctctggca catgaccgcc
     2221 tacctcctgg cgatcctcct gctgtggccg atggggttcg ggcgggagca ggacagcacg
     2281 ctccgctggt ggctggagcg gccgctgtgg atcctcgtcc ccgggctgat cctgctcgcg
     2341 atcgtggggc tggtggggcg gttcgagcga ggctcggtgc ggacccgggc cgcgcccccg
     2401 gaggagaggc tcagaacggc ttgaggatca tcagccacag gatcaggagc aggccgccga
     2461 agcccaccac catgatcacc atgggccggg aggacgagag gaggacgtcc agacggcctg
     2521 gatccggagg gccggcctcg atgcccttct tcctctgctc gtaggtctgg atcccggccg
     2581 cctcccgcac ctggttgtag tagcccgagg ccagcccgta catcgcgccc atgatgagga
     2641 tcaggagggc gatcgcggtc cagatccatc cctcgcccca ccagtccccc aggaagccgc
     2701 tgacgatgcc cccggccagc agcaggagga gggagacgta catgaagccc aggctcgagg
     2761 acgagaggtc cagcagggcc ttgactcgct ccgggctccg ttcggtccgg agccggatcg
     2821 acgccatcgc cgacgccccg tgcgccatga agaatccgaa ccccccgacg acgtgcagga
     2881 acacccacca gcggtacatc tcgcctccct cttctcgctc tcggcgggag ggtagcatcg
     2941 ctccgtggcg ccgcgcctca aacatcggct cgacgacgtc gtccgggcgc tcgtcacgcc
     3001 gcgggccgag cccggggctc tcccgctggt cgacgcggcc gccgggttcc ggcccggcga
     3061 tcgatccgcg gtctcctcga gctccgcatt cctcctggcg ctcgcggggt cggacgatgc
     3121 cgcggtccac ctgtccgccg ccgctgaagg cggcgatgct gtggccgcgt tcctggcgga
     3181 tggcctccgg aggatcgacg aggagctgga cggtctcgga gaccgggacg ccgcgctggc
     3241 cgagcggatg ggggcactgg ccgcgttcct ggagcgggag gacgccggga gctcggttga
     3301 cgagctggcc gagcgcatct gggccgtgtt cttccccgag ggcgtgggga tccgaggtca
     3361 cgaggaggct cgggccgcgg agctgcgggc tcggcgggcg atcgacatca cggagcgcgg
     3421 ccccgccgtg accgacgccg cgcgtcaggt cctgttcacc gtcaaccttc tgctgaccgg
     3481 accgccggcg ggcgtggagg tcgacgacct ggacctcccg agcgagatca caggcgccct
     3541 gcgggatccg ggtcctcagg cgttctggta cgaccacccg gtccagatcg gcatcgacgc
     3601 cccctcgagc gagctccgat acgggctggc ggccatggac cgggggatgg ccttcgagag
     3661 ggagcggggg gccgaccggc ccctgcgggt tgtggtctcg ctctccacga cgcacgagcg
     3721 cctccaggag atcgcccgcc cctacctcga gcacgaggtc cgcgagatgg gcgggctgga
     3781 gcacctcgag ctgtacgcgt tcggggaggc cgacgctgag cgtctcgtgg cgaaggtgct
     3841 cgccccggcg gccgaggcct tcctcgaccg gccccacgcg cgggagctcc tgggggtgct
     3901 gggcgtccgc gggccctacg ggcgccacca cagcttcggg aaggcgatcg cggccgtctg
     3961 gcacgtgctg atcgaccccg aagtggtggc gacgttcaag acggatctcg accacagctg
     4021 gccccaggac gagctcgtct cggagacggg ggcctcgctg ctggagcact tcgccttccc
     4081 cctctggggc gggaccggga tcgacgcgga agggcgccgc gtggagctcg gggggttcgc
     4141 cgcggcgctg gtcaacgagg cggacatcga ccaggggctg ttcacgaccg acgtccccgt
     4201 ccccgaccgg tcacccgagg ccaacgagct cctcttctgg agcgcgctgc cccagggtct
     4261 ctccaccctg gctgaggcct cgacgcgata cgggatcgac ggccccgacg gggtgaccac
     4321 ggcgatcgaa cgtgtccacg tccacgccgg aacggtgggg atcctcgtgg aggccctcag
     4381 gcggcaccgg cccttcacgc cgtccttcat cggccgggcc gaggaccagg cgtacatcct
     4441 ttcgaccctc tgggggtccg agccgaggct ggcgcacgtc caccaccgcg agctgatcca
     4501 gcggcacgac aagaaggcgt tcgcggacgc ggccatcgag gccgctcgcg tgggtacctg
     4561 ggtcggggac cacgagcggc tggtgctctt ctccgagctg gcgaggcacc tgggcccggg
     4621 ccgggacgag gtgaagacgg tggccgggcc cttcacggga gccttcatct cgcagatccc
     4681 ggccacggtg accctgctgc gcctgacgct gaaggccggg gtgctggcgg cgaaaggcgc
     4741 cggggacgag gccgaggagc tgctcgcgat ggcggcggag cggctggggc ggacgctggc
     4801 gttcgtggag gggggcgagc tcgccgagcg tctcgacgcc gagcgtcgag gatgggacct
     4861 gctctacgag acgctcggcg agctcgaacg gcgcctggcg gcgggggacg ccgaggcgga
     4921 gaggctggcc gaccgcgcac gccgcgtggt cgcggcgtgc gcggtcggct gaccgtcggt
     4981 cagacctcgc aggcgatctc ggtctcggcg tcgcagtcgt cggtgccggg acctccgttg
     5041 gcccggtcgt cgtcaccgtt ctggccgagg atgaagtcgt cgcctccctc gccgaagagc
     5101 cggtcgttcc cgtcgccgcc gaagaggtcg tcgttcccgg gtccgccgtg gaacacgttc
     5161 acgagcgcgg accccaccag gccgtccggc ccgtccccgc cgatcgcgtt ctcgacgtcc
     5221 gtcctgacgt tgtccccctc gcccgtgatg tagccgtcgt tggcgacgtc gtccaggacc
     5281 acgtcgacct tcgccgtgcg gcccacgtag ctgaccgtgt cgcggccggg tccgccacgg
     5341 aacatgtcgg cgccgaagcc tccgtccagc cggtcgcggc ccttcccccc gatcaggacg
     5401 tcgtcgccgc ccccggcgca gatccggtcg tggccgccct tcccccggat gacgtcgtcg
     5461 ccgcccttgg ccacgatcac gtcgttcctc ttcgtccccc ggatgacctc gcccttgctc
     5521 gtcccgacga tcgtggcccg cttgcccagg cacatcgggg cggcctgggc cgccggcatc
     5581 gtcatcgagg caagcacggc gaacgccgcc gtcaccaata cgacccttct cattcgctcc
     5641 gctccttggt caggtgccga tcaccagccc ggcgctccgg tagcacagca ggcttgccat
     5701 ggtggacccg ggccgctcgt cggtcaaccc gcggctgcgg cggggggtga ggctcgaggg
     5761 gcccacgccg agcggcaggt ggcacgcgaa ggtcgagcga agagggtgaa gggaacggtc
     5821 tgcgcggaag ccacgtctcg ctcgaccttc atcgcgctct tcctcgggcg cggcgctagc
     5881 ctggagagat gttcgggata acccgcgaga accttcgaca cttggcaacg accctgcgcc
     5941 tggggcctcg ctccgtgccg gcggtgtacg agagcctcgg gaccgacttc ttcctcgccc
     6001 tggcgcccgg atggctcaac ctgggctggt gggagggcga ggggaccgag gaggaggcgc
     6061 cgcgcgcccc ggtgcgcctc gtcgagaagc tggccgagcg gctccccccg ggcggcgtga
     6121 cgctcgacgt ggcgaacggg ctcggtgccc aggaccctgt catcgcccgc gtcgtgcggc
     6181 cacgcttcct ggccgccgtg aacgtcaccg agctccagct ccgcgcgggg agagggcggc
     6241 tggacgaggc ccgggcccgg cccgtgctgg ccgacgccgt ccgcctgccg ttcggggagg
     6301 acgccttcga cgggctgatc tcggtggagg ccgccttcca cttcccgtcc cgagccgcgt
     6361 tcttccgcga ggcggtgcgg gtcctgcggc cgggcggggt cctctcgatg tcggacctga
     6421 cggcggagcg gtggccgcgg ggggtccgcg acgcggccgc cgggctggcg cagctccgcg
     6481 tctggggcct gcgcgtccgc ccgctggcca gcggcggccg cgtcgcggct ctggcagagg
     6541 aggccgggct cgaggacgtc cgggtggagc tctgcgcgtc gaggacgatc gaccccgcca
     6601 tgcgcgtgat gcgggggagg ctcgcccacc ctcggggagc ctcccgggcc caggtgtggg
     6661 cctcgcgcgt ggccctgggc cagatcgagc gcctgcggga gggaggatac gtgcagtacg
     6721 cgctcctcac cgcgcgggcc cccgatcggg cgtaggaagg agagtcgacg cttgctccga
     6781 aatggccatt gcgtgcgggc tgctatgagg cgtagcatcc cggcgcccgc ccaagcggaa
     6841 ggagcatcgg gatgggtgcc tcgacggttc ggaaggtcgc cgccatcacc tcgctggtcg
     6901 tggccggcgt ggtggcgctg ctgctggcgc tacccggcgc ggaggcggcc ggaaagaagt
     6961 gcttcggcaa gacgcccacg atcaagagca acgccacggt gatcaagggc acgaacggca
     7021 acgacgtcat cctggccggg aagggtgaca accagatcta cggcaggaag gggttcgatc
     7081 tcatctgcgc cgggggtggc caggacttcg tcgacggagg cccagggagg agcaagatca
     7141 agggcggcgc cgggaaggac gccttccagg gcgggggcaa ggcggatgtc ttctacggcg
     7201 gcggaggaga cgacacgtcc gaccccggag gcggcccgga cgtgatctac ggcggccccg
     7261 gcaacgacgg gtcgaacaac gacctgggcg gcgatgccgg gaacgaccgc atctacggcc
     7321 aggatggcag cgacaacatc gacgggggcg acggcgacga cttctgcgat ggcggcgccg
     7381 gcgacgacaa cgcgtcggac tgcgagaccg aagtgtccat cccgtaacct cgcggggggc
     7441 cgggcccgct ccgggttccc ggag
//
LOCUS       DASUTW010000338         5140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1798434, whole genome shotgun sequence.
ACCESSION   DASUTW010000338 DASUTW010000000
VERSION     DASUTW010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1798434"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..1409
                     /gene="aroC"
                     /locus_tag="VF058_11640"
     CDS             246..1409
                     /gene="aroC"
                     /locus_tag="VF058_11640"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011606338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX6208993.1"
                     /translation="MRLLTAGESHGKALVAVLEGLPAGVPISEKEIEGELARRRHGYG
                     RSGRQRLEADRFEVLGGVRHGRTLGSPIAILIENAEWEAKWSRRMAPFGSPDPDDRLA
                     RPRPGHADLTGAQKYGFDDVRNVLERASARETVARVAAGAFCKAFLAELGMAVLSHVV
                     RIGGEEGELGTAPAPEDLPTIDASPVRCLDPDASERMVAAIDAARREGDSLGGVFEVV
                     AHGAPPGLGTYVHHERKLDARLALALMSIQAIKGVEVGHGFAGAARPGSQAHDEIEPG
                     PEGAGPRRRTGRSGGIEGGMSTGEPIRVRAAMKPLSTLPRPLDTVDLDTGQPAQAIRQ
                     RTDVCAVPAAAVVGEAVVAHVLAEAALEKFGGDSVEETRRNRDGYLRSLRGGA"
     gene            1406..1888
                     /locus_tag="VF058_11645"
     CDS             1406..1888
                     /locus_tag="VF058_11645"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX6208994.1"
                     /translation="MKVWLVGMPASGKTTTGTLLARRLGVPFVDVDQEIESRAGRDVA
                     GIFAEAGEAGFRSLEREAIALLAEDGDAVIAAGGGAVLDPANREAMRASGRVVLLEAP
                     AEVLVARLGDVPRPVTVGADLEALLRDRAEAYAAAAHLRVEADGPPEAIVDRIVEGLG
                     "
     gene            1885..2964
                     /gene="aroB"
                     /locus_tag="VF058_11650"
     CDS             1885..2964
                     /gene="aroB"
                     /locus_tag="VF058_11650"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX6208995.1"
                     /translation="MITVPVRVPGAAYDVVIGVGLLGRTGEVLPPFPGAERAFVVASQ
                     PVAERFLGTLEASLDLPAVHLAVPDGEEAKTLRTAEALWEHLATQGAHRSDPVMALGG
                     GSTGDVAGFVAATYMRGVPFVHLPTTLTAQVDAAIGGKVGVNLPRGKNLVGAFHQPAA
                     VLADVSTLAALPEEEFRSGLGEVAKYGLAVDPGVLDVLERRHGAVLARDPGALADLVA
                     ACVRAKASVVEADERDTGARAVLNYGHTLAHALERLEGYGRVSHGEAVAMGMAFAARL
                     SVTLGIGSEDLPARHRRVLASLGLPQSFPELDPEEVLEAMRLDKKYRRGLRFVLLEDV
                     GRPRVVDVRPEAIREVLSEALGGVA"
     gene            2961..3431
                     /locus_tag="VF058_11655"
     CDS             2961..3431
                     /locus_tag="VF058_11655"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX6208996.1"
                     /translation="MNVLLLLGPSLGALGSRDPDLYGDESLEDVMAGVSARAEELGHR
                     LTWRQSDAEGDLVGWLLAAREEGFEAVIANPGALTHYSHAVRDAVQASGLPAIEVHQT
                     NVFAREGFRRRSVISEACRAVIVGLGAGGYHVALEALGWITQRGEDGSPSGSTT"
     gene            3380..4447
                     /locus_tag="VF058_11660"
     CDS             3380..4447
                     /locus_tag="VF058_11660"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019047839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEX6208997.1"
                     /translation="MDHATRRGRLAERLDHLGVEAILITRVVNVRYLSGFTGSNAQLL
                     LSGDPVLLTDPRYDEQSSKEAPDLARRIYADGFRDELGAVLEAAGTSRLGFERHGVTY
                     ESYESLAELEGVELVPIGHEVERLRWEKEPEEIALLERAQEVTDAAFDEIVRKLAEGM
                     TEREVAFELELAMRRGGAERIGFDTIAAFGENAAEPHHGPTDRALVPGDLVKLDFGCV
                     VDGYHSDMTRTLAFGDPGDELREVYDIVARAQRAGEEAVRAGVVGGEADRAARRVIEE
                     AGHGDHFGHSLGHGVGLEIHEGPTLRRTSEDVLPAGAVVTVEPGIYLPGVGGVRIEDA
                     VVVEEGGCRPLPRTTKELIVL"
     gene            4444..5007
                     /gene="efp"
                     /locus_tag="VF058_11665"
     CDS             4444..5007
                     /gene="efp"
                     /locus_tag="VF058_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007433861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX6208998.1"
                     /translation="MNVSTNDLKTGMTLDLDDQLWQVVDFQHVKPGKGHAFVRTKLKN
                     VRSGAVLDRTFRADEKVGLAIVERNEMQYLYDDGHALVFMDTGSYEQIHVPKDVVGDM
                     AGYLVEGQTALIPMHEGVPIGVELPAAVVIEVAQTDPGVKGDRVSGAMKPAVLQTGVT
                     VQVPLFVGPGDKIKVDTRSGEYLTREQ"
BASE COUNT          688 a         1703 c         2079 g          670 t
ORIGIN      
        1 gagacgttcg ccgacaactt ccagctcgtc tcggtgcagg gagggtgcgc caacttccgg
       61 ttcggcgacc agtccttcac gctctgcgag ccgggagaga ggaagtgacg cccccgcgcg
      121 gcccgatggg acgcggccgg ggctgacccg cccggccggc gggatccgat ggctgcgacc
      181 gcggggggag cggccgtcgg agctgggagg cggccttcgg gccgcctcct ccgtatgctg
      241 gcacgatgag acttctgacc gcgggcgaat cgcacgggaa ggccctcgtg gccgtgctgg
      301 agggcctgcc ggcgggcgtc cccatctccg agaaggagat cgagggcgag ctggcacggc
      361 gacgccacgg ctacgggcgg agcgggcgcc agcggctgga ggccgatcgc ttcgaggtgc
      421 tcgggggggt gcggcacggg cggaccctgg gcagcccgat cgcgatcctg atcgagaacg
      481 cggagtggga ggcgaagtgg agccgccgga tggccccctt cggctccccg gatcccgacg
      541 accggctggc ccgtccacgc cccggccacg cggacctgac cggggcgcag aagtacggct
      601 tcgacgacgt ccggaacgtg ctggagcggg ccagcgcgcg cgagaccgtg gcccgcgtgg
      661 cggccggagc gttctgcaag gccttcctcg ccgagctggg gatggcggtc ctctcgcacg
      721 tggtgcggat cggaggcgag gagggcgagc tcgggaccgc tcccgcgccc gaagacctcc
      781 ccaccatcga cgcgtccccg gttcgctgcc tcgaccccga tgcctcggag cggatggtgg
      841 cggcgatcga cgcggctcgc cgcgagggcg acagcctggg cggcgtgttc gaggtcgtgg
      901 cgcacggcgc gccgccgggg ctcgggacct acgtgcacca cgagcggaag ctggacgcgc
      961 ggctggccct ggcgctgatg agcatccagg cgatcaaggg ggtggaggtg gggcacgggt
     1021 tcgcgggggc ggcgcggccc ggctcccagg cgcacgacga gatcgagccg ggccccgagg
     1081 gagccgggcc gcgccgccgc accggaaggt cgggtgggat cgagggcggc atgtcgacgg
     1141 gcgagccgat ccgggtgcgg gccgcgatga agccgctctc gacgctcccc cgccccctgg
     1201 acaccgtcga cctggacacg gggcagcccg cgcaggcgat ccgtcagcgg accgacgtgt
     1261 gcgcggtccc cgccgccgcg gtcgtagggg aggccgtggt ggcccacgtg ctggcggaag
     1321 ctgccctgga gaagttcggt ggcgactcgg tcgaggagac acgccgcaac cgcgacgggt
     1381 acctccgctc gctgaggggc ggcgcgtgaa ggtctggctg gtcgggatgc cggcctccgg
     1441 gaagaccacg acggggaccc tcctcgcccg gcgcctcggg gttccgttcg tggacgtgga
     1501 tcaggagatc gagtcccggg ccggccgcga cgtggcgggc atcttcgccg aggcggggga
     1561 ggcgggcttc cgctcgctgg agcgagaggc gatcgccctg ctggcggagg acggcgacgc
     1621 ggtgatcgcg gcgggaggag gcgccgtcct ggacccggcg aaccgcgagg cgatgcgggc
     1681 ctcgggccgc gtcgtcctgc tcgaggcccc ggcggaggtg ctggtggcca ggctggggga
     1741 cgtgccgcgc ccggtgaccg tcggcgcgga cctcgaggcg ctgctgcgcg accgcgccga
     1801 ggcctacgcc gccgccgcgc acctcagggt ggaggcggac gggccgccgg aggcgatcgt
     1861 cgaccgcatc gtcgaggggc tgggatgatc acggttcccg tccgcgtgcc cggggccgcc
     1921 tacgacgtcg tgatcggcgt cgggctgctg ggccggacgg gcgaggtcct gccccccttc
     1981 cccggcgccg agcgggcgtt cgtggtggcc tcccagcccg tggccgagcg gttcctgggg
     2041 acgctcgagg cctcgctcga tctgccggcc gtgcacctcg ccgtccccga cggcgaggag
     2101 gcgaagaccc tgcgcaccgc cgaggcgctc tgggagcacc tggcgaccca gggggcgcac
     2161 cgatcggacc ccgtgatggc gctgggcgga ggctcgaccg gcgacgtggc ggggttcgtc
     2221 gcggccacct acatgcgcgg cgtgccgttc gtccatctcc ccacgaccct cacggcccag
     2281 gtcgacgcag cgatcggggg aaaggtcggc gtgaacctcc cgcggggcaa gaacctggtg
     2341 ggcgccttcc accagccggc ggccgtgctg gccgacgtca gcacgctggc ggccctcccc
     2401 gaggaggagt tccgctcggg cctgggcgag gtggcgaagt acgggctcgc ggtggatccg
     2461 ggggtcctcg acgtgctgga gcgaaggcac ggcgccgtgc tggcccggga tccgggcgcg
     2521 ctcgccgacc tcgtggcggc ctgcgtgcgg gccaaggcgt cggtggtgga ggccgacgag
     2581 cgggacaccg gcgcccgggc ggtcctcaac tacggccaca cgctggccca cgccctggag
     2641 cggctggagg gatacgggcg ggtctcccac ggggaggcgg tggcgatggg gatggccttc
     2701 gcggcgcggc tctcggtgac gctcggcatc ggctccgagg acctcccggc gaggcaccgg
     2761 cgggtgctgg cctcgctcgg cctcccgcag tcgttccccg agctcgaccc cgaggaggtc
     2821 ctcgaggcga tgcgcctcga caagaagtac cggcggggcc tccgcttcgt gcttctggag
     2881 gacgtggggc ggccccgcgt ggtcgacgtg cggccggagg cgatccgcga ggtgctgagc
     2941 gaggcgctgg gaggcgttgc gtgaacgtgt tgctgctcct ggggccgagc ctcggcgccc
     3001 tgggctcccg ggacccggat ctgtacgggg acgagtcgct ggaggacgtg atggcggggg
     3061 tctcggcccg ggccgaggag ctgggccatc ggctgacctg gcgccagagt gacgctgagg
     3121 gggatctggt gggatggctg ctcgcggcgc gggaggaggg gttcgaggcg gtgatcgcga
     3181 accccggcgc gctcacccac tactcccacg ccgtgcggga cgcggtgcag gcctcggggc
     3241 tgcctgcgat cgaggtgcac cagaccaacg tcttcgcccg cgagggcttc cggcgtcggt
     3301 ccgtgatctc ggaggcgtgc cgggccgtga tcgtgggcct gggggccggc ggctatcatg
     3361 tggcgctgga ggcgttggga tggatcacgc aacgaggaga ggacggctcg ccgagcggct
     3421 cgaccaccta ggggtcgagg cgatcctgat cacccgggtg gtgaacgtgc gctacctctc
     3481 ggggttcacg ggctcgaacg cgcagctgct gctctcgggc gaccccgtcc tcctcacgga
     3541 tccccgctac gacgagcagt cctccaagga ggcgcccgac ctcgcccgcc gcatctacgc
     3601 cgatgggttc cgcgacgagc tcggggcggt gctggaggcg gcgggcacct cgcgccttgg
     3661 gttcgagcgt cacggtgtca cctacgagag ctacgagtcg ctggccgagc tcgagggcgt
     3721 ggagctcgtg ccgatcggcc acgaggtgga gcggctccga tgggagaagg agccggagga
     3781 gatcgccctc ctggagcggg cccaggaggt caccgacgcg gccttcgacg agatcgtgag
     3841 gaagctggcc gagggcatga ccgagcgcga ggtggccttc gagctcgagc tggccatgcg
     3901 gcgaggcggg gccgagcgga tcggcttcga cacgatcgca gccttcggcg agaacgccgc
     3961 cgagccgcat cacggcccga ccgaccgggc gctggtcccg ggggacctcg tcaagctcga
     4021 cttcggatgc gtggtggacg gctaccactc ggacatgacg cggacgctgg cgttcgggga
     4081 tccgggggac gagctccggg aggtctacga catcgtggcc cgggcccaga gggccggcga
     4141 ggaggcggtc cgggccgggg tcgtcggtgg ggaggccgac cgggcggccc gtcgcgtgat
     4201 cgaggaggcc ggccacggcg accatttcgg gcactcgctg ggccacgggg tgggtctgga
     4261 gatccacgag gggccgaccc tgcggaggac gtcggaggac gtgctccccg cgggcgccgt
     4321 agtgacggtg gagccgggca tctacctgcc gggcgtgggg ggcgtccgga tcgaggacgc
     4381 cgtggtggtc gaggagggcg gctgccggcc ccttcccagg accaccaagg agctgatcgt
     4441 gctgtgaacg tctcgacgaa cgacctgaag accgggatga ccctcgacct ggacgaccag
     4501 ctctggcagg tggttgactt ccaacacgtc aagccgggga agggccacgc cttcgtgcgc
     4561 acgaagctga agaacgtccg cagcggcgcg gtgctcgacc ggacgttccg ggccgacgag
     4621 aaggtgggcc tggccatcgt ggagcgcaac gagatgcagt acctctacga cgacgggcac
     4681 gcgctcgtgt tcatggacac cgggtcctac gagcagatcc acgtgccgaa ggacgtcgtg
     4741 ggcgacatgg ccggctacct cgtggagggc cagaccgccc tgatcccgat gcacgagggg
     4801 gtcccgatcg gcgtggagct gccggccgcg gtcgtgatcg aggtcgcgca gaccgatcca
     4861 ggcgtgaagg gcgaccgggt gagcggcgcg atgaagccgg cggtcctcca gacgggcgtc
     4921 acggtccagg tgccgctgtt cgtggggccg ggcgacaaga tcaaggtcga cacgcggtcg
     4981 ggcgagtacc tcacccggga gcagtgagat gggcgggcgg cgcggagccc gccgccgcgc
     5041 cgcgctcatc ctgtaccagg ccgacgtcac gcgccgggcg ccggccgagg tcctggagga
     5101 gcgggtcgag ctcggcgagg cgctcccctc gttcaccacg
//
LOCUS       DASUTW010000339         3456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_634871, whole genome shotgun sequence.
ACCESSION   DASUTW010000339 DASUTW010000000
VERSION     DASUTW010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634871"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VF058_11670"
     CDS             <1..209
                     /locus_tag="VF058_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6208999.1"
                     /translation="REGCVTVRFRLKVPARSTRYLLLFTEIGNSNEHNIQMARKFNKR
                     TLNRKVLAGISKGARSKIVNWDLT"
     gene            complement(252..899)
                     /locus_tag="VF058_11675"
     CDS             complement(252..899)
                     /locus_tag="VF058_11675"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX6209000.1"
                     /translation="MAERTMTKKERREAARAARLEAQKRARRKRLIRSWGIPLAVIAA
                     IALVAFITTMGDDGGPEVGEVEVSGPARQAALETGEPFPEFSATELEGGEVSWAPGGP
                     SLIAIWAPWCPICQEEIPMIDGLAPDFPEVEMVTVATAVDDRPGPTVSGFAEDEGLSL
                     PIALDDEAGTLARAMGIRGFPTVYLVDAEGAVVAAAEGQVGEEALREALDQLTAG"
     gene            complement(899..1393)
                     /locus_tag="VF058_11680"
     CDS             complement(899..1393)
                     /locus_tag="VF058_11680"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEX6209001.1"
                     /translation="MAVATMRAQGRVLRLLHLASRLALGGLLLWAGLAKVGDLQGMTL
                     SIDSYQILPEWSVRPLAAALPWIEVALGAFLVLGLFVRFSAVASGVLLVGFLIGMAQA
                     KARGLAIDCGCFGAGGPGEGVSWFDILRDVALIGVAGLVLLLPRDPWGLDARMDRLQH
                     GEET"
     gene            1488..2273
                     /locus_tag="VF058_11685"
     CDS             1488..2273
                     /locus_tag="VF058_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6209002.1"
                     /translation="MAHGDGSARDARSSGRIRAFRVIAVVMAVSAIAFGLFTAIFGAV
                     SESQEIHAYHNVIVASLLLVLSVPPVVAAAREPEGAAPHLVHLVTLGVAAVATMAIAL
                     MADVFTLPFVALAGVLLLLRVPREPAVGAGRPSIPLAIVVAVAAVPLLAYAIDQAGMQ
                     RVDDASEHAQLNHWVEVAFYAAAIPLLGALAAIRPRVFRLAGLTAGAALAILGGGSLL
                     LEGYPSSPDPPWGWAALGGGLLLLGLVEWEARRRASPDRVSAT"
     gene            complement(2266..3057)
                     /locus_tag="VF058_11690"
     CDS             complement(2266..3057)
                     /locus_tag="VF058_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014688983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX6209003.1"
                     /translation="MNDLRAVFRELHAQGLFLMPNPWDVGSARLLAWLGFRALATTSS
                     GHAASLGRLDQHVGRDELLAHAAAIVEAVDVPVSVDAERCFADDPEGVAETVRLIAGT
                     GAAGCSIEDYDPASGAIDPLEVAADRVAAAASAARAAGLVLTARAENHLYGLGDLGDT
                     IGRLRAYREAGAEVVYAPGLLDLDEIARVVREVEAPVNVLARPRGPSIPELASVGVRR
                     VSTGGALAMAAYGSLVSAARELLDAGTSSYLAGAVGDDALEAAFR"
     gene            3159..>3456
                     /locus_tag="VF058_11695"
     CDS             3159..>3456
                     /locus_tag="VF058_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6209004.1"
                     /translation="MDYKLELVVLPVGDVDRAKAFYADRAGFHLDVDTTPAEGFRVVQ
                     LTPPGSACSITFGTGLTDAEPGAYKGLHLVVSDIVAAREELVGRGVEVGEIRHMS"
BASE COUNT          476 a         1322 c         1199 g          459 t
ORIGIN      
        1 atcgcgaggg gtgcgtgacc gtgcggttcc gtctcaaggt cccagcgagg tccacgaggt
       61 acctcctgct gttcacggag atcgggaaca gcaacgagca caacatccag atggcgagga
      121 agttcaacaa gcgaacgctg aaccggaagg tcctcgccgg catcagcaag ggcgcgcgct
      181 cgaagatcgt gaactgggac ctgacctgac ctcgcgggcg ccccgggccg cagcccgggg
      241 cgcccgcctc ctcagccggc ggtcagctgg tcgagggcct cccggagggc ctcttcgccg
      301 acctggccct ccgcggccgc cacgaccgcc ccttcggcgt ccaccaggta gaccgtcggg
      361 aaccctcgga tccccatggc cctcgccagc gtcccggcct cgtcgtcgag ggcgatcggg
      421 aggctgagcc cctcgtcctc cgcgaacccc gacaccgtcg gcccgggccg gtcgtcgacc
      481 gcggtggcca ccgtcaccat ctcgacctcc gggaagtcgg gcgccagccc gtcgatcatc
      541 gggatctcct cctggcagat cgggcaccag ggggcccaga tcgcgatcag cgagggcccg
      601 ccgggcgccc acgacacctc gccgccctcg agctcggtcg cggagaactc ggggaacggc
      661 tcgccggtct ccagcgccgc ctgccgggcc gggccggaga cctccacctc gccgacctcc
      721 gggccaccgt cgtcccccat cgtggtgatg aaggcgacga gcgcgatcgc ggcgatgacg
      781 gcgaggggga tcccccacga gcggatcagg cgcttccgcc tggcccgctt ctgagcctcg
      841 agcctcgccg ccctggccgc ctcccgacgc tccttcttgg tcatggtgcg ctcagccatc
      901 acgtctcctc tccgtgctgc agccggtcca tcctcgcgtc cagcccccac ggatcgcgcg
      961 gcagcagcag gacgagcccc gccacgccga tcagcgccac gtcccgcagg atgtcgaacc
     1021 agctcacgcc ctcgcccgga cctcccgccc cgaagcagcc gcagtcgatg gcgaggccgc
     1081 gggccttcgc ctgcgccatc ccgatcagga agcccacgag cagcaccccg ctcgccaccg
     1141 cggagaatcg aacgaagagc ccgagcacga ggaacgcgcc cagcgcgacc tcgatccacg
     1201 gcagggccgc ggccagcggc cgcaccgacc actccggcag gatctggtac gagtcgatcg
     1261 agagggtcat cccctgcagg tccccgacct tggccagccc cgcccagagc agcagcccgc
     1321 ccagggcgag cctggaggcg aggtggagga gccggaggac cctcccctgg gcgcgcatcg
     1381 tcgcgaccgc catacgggca aggatatcgg cggcgcgcgg ggtgccgacc cgggccggag
     1441 gtccccccac gggcggtccc accggccccc cgtgtataac tgggtccgtg gcgcacggcg
     1501 acggctcggc tcgcgacgcc cgatcgagcg ggcggatccg cgccttccgc gtgatcgcgg
     1561 tggtcatggc cgtctcggcc atcgccttcg ggctcttcac ggccatcttc ggggccgtca
     1621 gcgagtcgca ggagattcac gcctaccaca acgtgatcgt ggcctcgctg ctcctggtgc
     1681 tctccgtgcc gcccgtcgtg gcggccgctc gcgagccgga gggagccgcg ccgcacctcg
     1741 tccacctcgt gacgctcggg gtcgcggccg tggccacgat ggcgatcgcc ctgatggcgg
     1801 acgtcttcac gctccccttc gtcgcgctgg cgggggtgct cctcctcctg cgagtgccgc
     1861 gcgagccggc ggtgggggcc ggacgcccca gcatccccct ggcgatcgtc gtggccgtgg
     1921 ccgccgtgcc cctgctcgcc tacgcgatcg accaggccgg catgcagcgg gtggacgacg
     1981 ctagcgagca tgcccagctc aaccactggg tcgaggtcgc cttctacgcg gcggccatcc
     2041 ccctgctcgg cgcgctggcg gcgatccgtc cccgcgtctt ccggctggcc ggcctgaccg
     2101 cgggcgccgc gctggccatc ctgggcggcg gctcgctcct cctggaggga tacccatcgt
     2161 ccccggaccc gccctggggc tgggcggcgc tgggcggggg cctcctcctc ctgggcttgg
     2221 tggagtggga ggcgcgtcgc cgggcgagcc ccgaccgggt ctccgctacc tgaacgcggc
     2281 ctccagggcg tcgtcgccca ccgccccggc caggtacgac gacgtgccgg cgtcgaggag
     2341 ctcccgggcg gccgagacca ggcttccgta ggcggccatc gccagcgccc cgccggtgga
     2401 gaccctgcgc acgccgaccg aggcgagctc ggggatcgag ggtcccctgg ggcgggcgag
     2461 gacgttgacc ggcgcctcca cctcgcgcac gacccgagcg atctcgtcca ggtcgaggag
     2521 cccgggagca tagacgacct ccgcccccgc ctcccggtac gcacgcaacc tgccgatcgt
     2581 gtcgcccaga tccccgaggc cgtagaggtg gttctcggcc cgagccgtga gcacgaggcc
     2641 ggcggcgcgc gcggcgctcg ccgcggcggc cacccggtcc gccgccacct cgagcgggtc
     2701 gatcgctccg ctggcgggat catagtcctc gatcgagcag ccggcggcgc ccgtccccgc
     2761 gatgagccgc acggtctccg ccacgccctc gggatcgtcg gcgaagcacc gctcggcgtc
     2821 cacgctcacc gggacgtcca ccgcctcgac gatcgctgcg gcgtgggcca gcagctcgtc
     2881 gcgaccgacg tgctgatcca gccgcccgag cgaggcggcg tgcccggagc tggtcgtggc
     2941 cagggcccgg aagccgagcc aggcgagcag acgggccgac cccacgtccc agggattcgg
     3001 catcaagaac agcccctgcg cgtggagctc ccggaagacg gcacggagat cgttcatcga
     3061 tcgtctcctt gatgagcgga tggtcgatcg tatcgaggag cgcgatgaga tgcgccgcgc
     3121 cgtagggtcg agatgtcgag accgagaagg aggatccgat ggactacaag ctcgagctcg
     3181 tggtgctgcc ggtgggcgac gtggatcggg ccaaggcctt ctacgccgat cgagccggct
     3241 tccacctcga cgtggacacg acccccgccg aggggttccg ggtcgtgcag ctcacccctc
     3301 cgggctcggc ctgctcgatc acgttcggca cggggctgac cgacgccgag ccgggggcct
     3361 acaagggact gcacctcgtc gtctcggaca tcgtcgccgc ccgcgaggag ctcgtggggc
     3421 gaggcgtcga ggtgggggag atccgccaca tgtcgt
//
LOCUS       DASUTW010000340         8260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_894079, whole genome shotgun sequence.
ACCESSION   DASUTW010000340 DASUTW010000000
VERSION     DASUTW010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8260
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894079"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VF058_11700"
     CDS             <1..330
                     /locus_tag="VF058_11700"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX6209005.1"
                     /translation="ELPLWRQLAATPASVRRRARALAKGLDDAEAVEAETVVGGGSLP
                     GYALPSWAVRLPVPDPDRTAATLRAGNPPVFCRVDEDALLFDLRTVEPDDDERLARAI
                     GYALAQG"
     gene            327..2186
                     /gene="selB"
                     /locus_tag="VF058_11705"
     CDS             327..2186
                     /gene="selB"
                     /locus_tag="VF058_11705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEX6209006.1"
                     /translation="MSEPLHVVATAGHVDHGKSSLIVRLTGIDPDRWEEEKRRGLTID
                     LGFAWCALPSGREIGFVDVPGHERFIRNMLAGMGPVKLVLFVVAADEGWKPQSEEHLA
                     IADVLGAEAGVVALTKRDLVDEARLAERADEVRARLAGTVLEAAPVVPCSAATGEGLD
                     DLRGALDEMVAAAPRPEDRGRPRLFVDRSFTIKGSGTVVTGTLTGGTLSVGDEVTLLP
                     SGARTRVRSLQTHRRSIEVARPVSRVAANLAGTATDEVERGDALVRPGQWRPTSAIEV
                     RLRAVRSLGDPLTNRGAFKLHLGSAERDASLRLYGTRELAAGEESLARVRLSGPVVAA
                     VGDRFVLREVGRRETVGGGVVLDTDPPRRADEARLARRERAPIEDLPALLVAERGAVR
                     VSEIEPLTGGSPAEIRGAARLGSWWVHDDALGRLRDAVSEALEAHHRDQPLLPGMDRA
                     VLRDRLALAEPSLAPHLEADLAAALLEHLEGEGFIAREGPLLRLADHRVTLGPREEEA
                     ARLVAAVQDGEPTPPDVPTLLGEGFDRELIDAVCRVGRLARVSREVVVTPDFLARAER
                     VAREQAGSGEGLTVSGFRQALGTTRKYALPLLGSFDERGITRRDGDVRRIVEA"
     gene            complement(2190..2642)
                     /locus_tag="VF058_11710"
     CDS             complement(2190..2642)
                     /locus_tag="VF058_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6209007.1"
                     /translation="MIEERLQELGIELPPAAAPVASYVPVKRGPGIAFVSGQVPMEDG
                     APIVTGKLGAEVALERGVELARRCALQALAALRDELGSLDAVKQVLRVGIFVASAPDF
                     TDQPKVGNGASDLLVEVFGEAGKHARAAVGCPSLPLDVPVEVELTVEI"
     gene            complement(2644..2916)
                     /locus_tag="VF058_11715"
     CDS             complement(2644..2916)
                     /locus_tag="VF058_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007030715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX6209008.1"
                     /translation="MNEWVMGWQYRAACRGEDSSVFFAPNYFERKDEKEAREAKAKAL
                     CVRCPVRLECLDYALRTREPHGIWGGLNEVERRALIREREGTRQAG"
     gene            3153..3833
                     /locus_tag="VF058_11720"
     CDS             3153..3833
                     /locus_tag="VF058_11720"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6209009.1"
                     /translation="MDLSEETFERVFETARALGEGTRFRIFRRLCGAAPEPVSVRDLT
                     ESFDLHPNAIRQHLARLEQAGLVTSSPRHEGGAGRPRRVYLATPEGLRVGATRGSPGA
                     LLAVLEDALQALPADRETLVEFGRAWGRAWARRRRSESGPTRGRRARAELLATELAIW
                     GWEPTTDRTNGTIKVATGRCLFRGDARGRKGRCCALEEGFLSGLAEGLLNGSAVVNLD
                     GCRLALSV"
     gene            complement(3830..4681)
                     /locus_tag="VF058_11725"
     CDS             complement(3830..4681)
                     /locus_tag="VF058_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003995999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX6209010.1"
                     /translation="MSTGMIVLLVILGIVVLLIIAAIRIVQEYERGVIFRLGRVLPAP
                     KGPGLFFIIPIIDRVVKVNLQTVTMNVPPQDVITRDNVTVRVDAVVYFHVVEPIKATV
                     EIQNYLVGTSQIAQTTLRSVLGKAELDDLLAERERLNDELQTVIDELTEPWGVKVTLV
                     EIKDVDLPDNMQRAMARQAEAERIRRAKVINAEGEFQAAAKLSEAAGVIARHPIAYQL
                     RLLQTMAEVSAEKNSTLVLPIPIELLRFFERARPVIEEGGGDPEEIGRALREAGVAEE
                     AEPEEEA"
     gene            complement(4678..6021)
                     /locus_tag="VF058_11730"
     CDS             complement(4678..6021)
                     /locus_tag="VF058_11730"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX6209011.1"
                     /translation="MRRLGLVLMAAGVLLGGPQAAAQENAAYVDVIELEGVIDPTTAD
                     YLADEIDRSVETGSGLVVIQIDTPGGLDVSMRDMVATILASEVPVAVWVGPPGARAAS
                     AGVFLVYASHVAAMAPATNIGAAHPVNLGGGETPDAAEEKAVNDAAKFLRSLAERRGR
                     PTDFAEAAVRESESLSAQEAVDREVIEVLSPSLTDLLAQLDGREVQTDRGTETLVTVA
                     TEDRPLEIRFHQMGLLRQILHAVSNPTVAFLLLTLGFWAIIFEISQPGFGIAGVGGAI
                     ALVLAFYALAVLPVNLAGLILVLLGLVLFTIDVFVQGLGVFTVGGALAFGIGGFLLFA
                     GASPEFRISPWVIVGLLIGSVLFFGFAMTAALRARRRASMTGQEGLVGLTGEAKADLD
                     PEGHVWVKGALWKARALGEPIPEGSRIRVRRVDGLLLLVQREEQKETAGAREGEA"
     gene            complement(6061..6342)
                     /locus_tag="VF058_11735"
     CDS             complement(6061..6342)
                     /locus_tag="VF058_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018512587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX6209012.1"
                     /translation="MSWEERAACGGFDPELFFGNTAADERRAKAICGTCAVAVECLSV
                     ALTSGMDFGIWGGLNERERRQVRRRNPGTSDWRALIEERGIRRQPVRAG"
     gene            6482..>8260
                     /locus_tag="VF058_11740"
     CDS             6482..>8260
                     /locus_tag="VF058_11740"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX6209013.1"
                     /translation="MATTHRAVTRRVRAADLERVRGLPDQALRVILLVFVVAIAAGLF
                     ALALLPGVGAAGRGVQRFNDELSTIGTDIEIRYPRFPERSTIFAADGTVLATLFLDEN
                     RKIVTLRNVDRDLIDAVLAIEDARFYEHPGLDYRGIIRALVTNVGAGDIEQGASTLTQ
                     QLARNVYADVGTERTLARKIAEARVAMRMEDEYSKDQILELYLNEIYMGRSVYGVGTA
                     AEYYFGKHVRNLELHEAAMLAGLIQAPERYSPVVDKEAALSRRNVVLDRMAELGMITP
                     QEAGQAKGRRLGLQPKNVVEKRKFPFFVEFVRREILDLENKAFDELGTTARQRQRALF
                     QGGLQIHTTLEPKLQRAGERVSRQHLPLPEDPQNAIASVEAGTGRILALVSSQEFERS
                     QVNLATGQGGSGRQAGSAFKPFTLVTAFEQGIPPTKVYNGANGQTIPACGDYRLVNAD
                     GGGGVMDIWAATRGSVNAVFVQLAVDAGLEANVEVAHRMGIESPLLAVCSLPLGTNEV
                     TPLEMASAYATLANGGKRCERHAIDTIENRRGDVIVKQKRRCTQVVDPVIAALTVNLL
                     EGVIEAGTGTAANLGTWPAFGKTGSTN"
BASE COUNT         1170 a         3048 c         2904 g         1138 t
ORIGIN      
        1 gagctgccgc tttggcggca gctcgccgcg acgcccgcct cggttcgcag gcgggcccgg
       61 gccctggcca agggcctgga cgacgcggag gccgtggagg ccgagaccgt ggtgggcggc
      121 ggatcgcttc cgggctacgc cctcccttcg tgggccgtgc gcctccccgt cccggaccca
      181 gaccgcacgg ccgccacgct ccgtgcgggc aaccctccgg tcttctgccg ggtcgacgag
      241 gacgcgctcc tcttcgacct gcggaccgtc gagcccgacg acgacgagcg cctggcccgc
      301 gccattggct acgccctggc ccagggatga gcgagccgct ccacgtggtg gccacggcgg
      361 gccacgtcga tcacggcaag tcctcgctga tcgtccgcct caccggcatc gaccccgacc
      421 gctgggagga ggagaagcgc cgcgggctca cgatcgatct cggcttcgcc tggtgcgcgc
      481 tcccctcggg ccgggagatc gggttcgtgg acgtccccgg ccacgaacgc ttcatccgca
      541 acatgctcgc cgggatgggc cccgtgaagc tcgtgctgtt cgtcgtggcg gccgacgagg
      601 gatggaagcc gcagtccgag gagcacctgg cgatcgccga cgtgctgggc gccgaggcgg
      661 gggtcgtggc cctgacgaag cgcgacctgg tggacgaggc gcggctggcg gagcgggctg
      721 acgaggtccg ggcccggctc gccggcaccg tcctggaagc ggcccccgtc gtcccctgct
      781 ccgccgcaac cggcgagggc ctggacgacc tccgcggcgc cctcgacgag atggtggccg
      841 ccgcgccgag gcccgaggac cggggacgcc ctcggctgtt cgtggaccgc tcgttcacga
      901 tcaagggatc cgggaccgtc gtcaccggga cgttgaccgg tgggacgctc tccgtcggcg
      961 acgaggtgac gctcctcccc tccggcgcca ggacccgcgt ccgatccctg cagacccatc
     1021 gccggtcgat cgaggtggcg cggcccgtct cgcgcgtcgc cgcgaacctc gcgggcaccg
     1081 ccaccgacga ggtggagcga ggcgacgccc tcgtccgccc cgggcagtgg cggcccacga
     1141 gcgcgatcga ggtccgcctc cgcgcagtcc gctcgctcgg cgatccgctc acgaaccggg
     1201 gggccttcaa gctgcacctc ggctcggccg agcgcgacgc ctcgctccgg ctctacggga
     1261 cccgagagct cgccgcgggc gaggagtcgc tggcccgggt ccggctctcg ggcccggtcg
     1321 tggccgccgt gggcgaccgg ttcgtgctcc gcgaggtggg gcggcgcgaa accgtcggcg
     1381 gcggcgtggt cctggacacc gatccgcccc gacgggccga cgaggcgcgt ctggcccggc
     1441 gggagcgcgc cccgatcgag gacctccccg cgctcctcgt cgcggagcga ggcgcggtgc
     1501 gggtctcgga gatcgagcct ctcacggggg ggtcgcccgc agagatccgc ggcgcggccc
     1561 gcctgggatc gtggtgggtg cacgacgacg cgctggggag gctccgggac gccgtctccg
     1621 aagccctgga agcccaccat cgggaccagc ccctcctccc ggggatggac cgtgccgtgc
     1681 tccgggaccg cctcgccctg gccgagccct cgctcgcgcc acacctggag gccgacctcg
     1741 ccgcggcgct cctggagcat ctggagggcg agggcttcat cgcccgggag gggccgctcc
     1801 tgcggctcgc cgatcaccgc gtcacgctcg gcccccgcga ggaggaggcc gcccggctcg
     1861 tggcggcggt ccaggacggc gagcccacgc cgcccgacgt cccgaccctg ctcggggagg
     1921 gcttcgaccg cgagctgatc gacgcggtct gccgggtggg gcgcctggcc cgcgtctcca
     1981 gggaggtggt cgtgacgccc gacttcctgg cgcgggccga gcgggtggcc cgagagcagg
     2041 cgggatccgg ggaaggcctg acggtctccg gcttccgtca ggccctcggc acgacgagga
     2101 agtacgccct ccccctgctg ggctccttcg acgagcgagg gatcacgcgg cgagacggcg
     2161 acgtccgccg catcgtggag gcctgagcct cagatctcga cggtgagctc gacctcgacg
     2221 gggacgtcga gcgggaggga ggggcacccc acggcggccc gcgcgtgctt gcccgcctcc
     2281 ccgaagacct ccacgaggag atcgctggcg ccgttcccca ccttgggctg gtcggtgaag
     2341 tccggggccg aggccacgaa gatcccgacg cgcagcacct gcttcacggc gtcgagcgag
     2401 ccgagctcgt cgcggagcgc cgccagagcc tggagcgcgc accgcctggc cagctcgacg
     2461 ccccgctcga gcgcgacctc ggccccgagc ttccccgtga cgatgggagc gccgtcctcc
     2521 atcgggacct ggcccgagac gaaggcgatc ccgggacccc gcttcaccgg gacgtaggag
     2581 gccaccggcg cggcggccgg cggcagctcg atccccagct cctgcaggcg ctcctcgatc
     2641 acgtcagccg gcctgccggg tcccctcgcg ctcccggatc agcgccctgc gctcgacctc
     2701 gttgaggccg ccccagatgc cgtggggctc acgggtcctg agggcgtagt cgaggcactc
     2761 gagccggacg gggcagcgca cgcagagcgc cttggccttc gcctcgcggg cctccttctc
     2821 gtccttgcgc tcgaagtagt tcggcgcgaa gaagacgctg gagtcctcgc cgcggcaggc
     2881 ggcgcggtac tgccacccca tcacccactc gttcatcacg actcggtccc tcacccgttc
     2941 ttcgtatgga aacgcctcgg cggttcgcta catttctccc ccggccgagg cgaccccgtt
     3001 tctccaattt cacctgtgga aatgtccttg ggcgcagggt atcaggggtc cctgagcgtt
     3061 tgtcaacccc cgatccggcg gcggcgaggg gcggccgggg gaagccccct cggccccatc
     3121 cggatcgagg cgcgggtcta tcatcgaggc gcatggacct atccgaggag acgttcgagc
     3181 gggtcttcga gaccgctcgg gccctggggg aggggaccag gttccgaatc ttccgacggc
     3241 tgtgcggggc cgccccggag ccggtctcgg tccgcgacct cacggagagc ttcgacctcc
     3301 accccaacgc gatcaggcag cacctggccc ggctggagca ggccgggctc gtgacgtcga
     3361 gcccccgcca cgagggcggg gccggtcggc cccgccgcgt ctacctggcc acgcccgagg
     3421 ggctgagggt cggcgccacc cggggaagcc cgggcgcgct gctggcggtg ctggaggacg
     3481 ccctgcaggc cctgcccgcc gaccgcgaga cgctggtgga gttcggccgc gcgtggggcc
     3541 gcgcctgggc tcggcggcgc cggagcgaga gcggccccac gcgcggccga cgagcccggg
     3601 ccgagctgct cgccaccgag ctcgccatct ggggatggga gcccacgacc gatcgcacca
     3661 acgggacgat caaggtcgcc accggccgct gcctcttccg cggggatgcc cgcgggagga
     3721 agggcaggtg ctgcgcgctc gaggaggggt tcctctcggg cctggccgaa ggcctgctga
     3781 atgggtccgc cgtcgtgaac ctcgacggct gccgcctggc cctctcggtc taggcctcct
     3841 cctcgggctc cgcctcctcg gccacccccg cctcgcggag cgccctcccg atctcctcgg
     3901 gatccccgcc gccctcctcg atcaccggcc tggcccgctc gaagaagcgc aggagctcga
     3961 tggggatcgg caggacgagc gtcgagttct tctcggcgga gacctctgcc atcgtctgca
     4021 ggaggcgcag ctggtaggcg atggggtgcc gggcgatcac accggccgct tcggacagct
     4081 tggccgcggc ctggaactcg ccctcggcgt tgatgacctt ggcccggcgg atccgctccg
     4141 cctcggcctg ccgagccatg gcccgctgca tgttgtcggg caggtcgacg tccttgatct
     4201 ccacgagcgt gaccttgacc ccccagggct cggtcagctc gtcgatcacg gtctggagct
     4261 cgtcgttcag ccgctcgcgc tcggcgagca gatcgtcgag ctcggccttc cccagcaccg
     4321 accgcagggt cgtctgcgcg atctgcgacg tccccacgag gtagttctgg atctcgaccg
     4381 tggccttgat cggctccacg acgtggaagt agacgacggc gtccacccgg acggtgacgt
     4441 tgtcccgggt gatgacgtcc tggggcggga cgttcatcgt caccgtctgg aggttcacct
     4501 tcaccacccg gtcgatgatc gggatgatga agaagagccc cggccccttc ggcgcgggca
     4561 ggacccggcc cagccggaag atcacccctc gctcgtactc ctgcacgatg cggatcgccg
     4621 cgatgatcag cagcacgacg atccccagga tcacgaggag cacgatcatg cctgtgctca
     4681 tgcctcaccc tccctcgctc cggcggtctc cttctgctcc tcccgctgca cgagcagcag
     4741 gaggccgtcc acccgtcgca cccggatgcg gctcccctcc gggatcggct cacccagcgc
     4801 ccgtgccttc cacaacgctc ccttgaccca gacgtgcccc tcggggtcga ggtcggcctt
     4861 ggcctccccg gtcaggccga cgagcccctc ctgcccggtc atcgaggctc tccgccgggc
     4921 ccggagggcg gcggtcatgg cgaagccgaa gaacagcacg ctcccgatca gcaggcccac
     4981 gatcacccac ggcgagatcc ggaactcggg cgaggccccc gcgaacagca ggaacccccc
     5041 gatgccgaag gcgagcgcgc ccccgaccgt gaacaccccc aggccctgca cgaagacgtc
     5101 gatcgtgaac agcacgaggc ccagaagcac caggatcagg ccggccaggt tcaccgggag
     5161 cacggccagg gcgtagaagg cgaggacgag cgcgatcgcc ccgcccaccc cggcgatccc
     5221 gaaccccggc tggctgatct cgaagatgat ggcccagaac cccagggtca ggaggaggaa
     5281 cgccaccgtc gggttcgaga ccgcgtggag gatctgccgc agcagcccca tctgatggaa
     5341 gcggatctcc aggggccgat cctcggtggc caccgtgacc agcgtctcgg ttccccggtc
     5401 ggtctggacc tcacgcccgt cgagctgggc caggaggtcg gtgagcgacg gcgagagcac
     5461 ctcgatcacc tcgcgatcca cggcctcctg ggccgagagc gactcgctct cccggaccgc
     5521 cgcctccgcg aagtcggtgg ggcgcccgcg gcgctcggcc aggctccgta ggaacttcgc
     5581 cgcgtcgttc acggccttct cctcggccgc gtcgggtgtc tcgccgcctc ccaggttcac
     5641 ggggtgcgcc gccccgatgt tggtggcagg ggccatcgcc gcgacgtggg aggcgtagac
     5701 gaggaagacc cccgccgacg cggcccgcgc ccccggagga cccacccaca ccgccacggg
     5761 gacctcggag gcgaggatcg tggcgaccat gtctcgcatc gagacgtcga ggccgccggg
     5821 ggtgtcgatc tggatcacga cgaggcccga gccggtctcc accgatcgat cgatctcgtc
     5881 ggcgaggtag tcggcggtgg tggggtcgat gacgccctcg agctcgatga cgtcgacgta
     5941 cgcggcgttc tcctgcgcgg cggcctgcgg cccgccgagc aggacgccgg ccgccatcag
     6001 gacgagcccg agcctcctca cgccccctat cctagggggc gtctcccccc ttcgccgggc
     6061 tcagccggcg cggacgggct gacggcggat cccacgctcc tcgatcaggg cccgccagtc
     6121 gctcgtcccc gggttgcgcc gccgaacctg acggcgctcc cgctcattga gaccccccca
     6181 gatgccgaag tccatgccgg aggtcagggc gacgctcaga cactcgacgg ccaccgcgca
     6241 ggtcccgcag atcgccttgg cgcgccgctc gtcggccgcc gtgttgccga agaagagctc
     6301 gggatcgaag ccgccgcagg ccgcccgctc ctcccaggac atggcccggc atggtagtcg
     6361 cgcgatagtg cgctgtggat agtccgaacg gcccaatttt cctccacacg gtttccacag
     6421 gtttcccacc agaactccca tggccgcgga accgatcgct cgctatcctg ttccggatcg
     6481 catggccacc acccaccgag ccgtcacacg tcgcgtgcga gccgccgacc tcgagcgcgt
     6541 ccggggactc cccgatcagg ccctccgggt catcctgctc gtcttcgtcg tggcgatcgc
     6601 ggctgggctc ttcgcgctgg cgctgcttcc gggcgtgggg gcggcgggcc gcggggtgca
     6661 gcgcttcaac gacgagctca gcacgatcgg gaccgacatc gagatccgtt acccgcgctt
     6721 cccggagcgc tccacgatct tcgccgccga cggcacggtg ctggccacgc tcttcctcga
     6781 cgagaaccgc aagatcgtga cgctgcggaa cgtggaccgg gacctgatcg acgcggtgct
     6841 ggcgatcgag gacgcccgct tctacgagca ccccggcctc gactaccgcg ggatcatccg
     6901 ggccctcgtc acgaacgtgg gggcaggcga catcgagcag ggcgcctcga ccctcaccca
     6961 gcagctcgcg cggaacgtgt acgcggacgt cgggaccgag cgcacgctgg cccggaagat
     7021 cgccgaggcc cgcgtggcca tgcgcatgga ggacgagtac tcgaaggacc agatcctcga
     7081 gctctacctc aacgagatct acatgggccg gagcgtctac ggggtcggta cggccgccga
     7141 gtactacttc gggaagcacg tgcgcaacct cgagctgcac gaggccgcga tgctggccgg
     7201 gctgatccag gcgcccgaac gctactcgcc cgtcgtggac aaggaggccg cgctcagccg
     7261 gcgcaacgtg gtgctcgacc gcatggccga gctcgggatg atcacccccc aggaggcggg
     7321 gcaggcgaag ggcaggcgtc tgggcctcca gcccaagaac gtcgtggaga agcggaagtt
     7381 cccgttcttc gtggagttcg tccgccggga gatcctcgac ctggagaaca aggccttcga
     7441 cgagttgggg accaccgccc gccagcgcca gcgcgcgctc ttccagggcg ggctgcagat
     7501 ccacaccacg ctcgagccga agctccagcg ggccggggag cgggtgtcac ggcagcacct
     7561 ccccctcccc gaggaccccc agaacgcgat cgcctcggtc gaggcgggca ccggccggat
     7621 cctggccctc gtctcgagcc aggagttcga gcgctcgcag gtgaacctgg ccacgggaca
     7681 ggggggctcg gggcggcagg ccgggtcggc tttcaagccg ttcacgttgg tcacagcctt
     7741 cgagcagggc atcccgccaa cgaaggtcta caacggggcc aacggccaga cgatccccgc
     7801 atgcggcgac tacaggctgg tgaacgcgga cgggggcggg ggggtcatgg atatctgggc
     7861 cgccacgcgc ggctcggtga acgcggtgtt cgtgcagctg gccgtggacg ccgggctgga
     7921 ggccaacgtc gaggtcgctc accgcatggg catcgagagc cccctgctcg cggtgtgctc
     7981 gctccccctg ggcacgaacg aggtcacgcc gctcgagatg gcgtccgcct acgccaccct
     8041 cgccaacggc ggcaagcgct gcgagcggca cgcgatcgac acgatcgaga accggagagg
     8101 cgacgtgatc gtgaagcaga agcgtcggtg tacgcaggtc gtcgaccccg tgatcgcggc
     8161 gctcaccgtc aatctgctgg agggcgtgat cgaggccggg accgggacgg cggccaacct
     8221 ggggacctgg cccgcgttcg gcaagacggg ctcgacgaac
//
LOCUS       DASUTW010000341         3566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1670813, whole genome shotgun sequence.
ACCESSION   DASUTW010000341 DASUTW010000000
VERSION     DASUTW010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1670813"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VF058_11745"
     CDS             <1..221
                     /locus_tag="VF058_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6209014.1"
                     /translation="EVLTWPAGPEEIEKAMRLVPDEVVQMITASGTAEECRAKVREYV
                     EAGCTCPVLYPLGDDVGAMLDAFAGGAF"
     gene            224..1099
                     /locus_tag="VF058_11750"
     CDS             224..1099
                     /locus_tag="VF058_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX6209015.1"
                     /translation="MEVLRPRDLREALAMKAAHPEARPIAGGTDLMVELNFDRDRPEA
                     ILDLWPVPELREHGREDGTIFLGAGVTFARIMRELPELVPLYQASRTVGSPQIRNRGT
                     IGGNLGTASPAGDALPVLAAYDAEVVVGSEGADPRSLPWSEFLRGPKQTTLGPEELIL
                     GVRFRPPRGPGSFSKIGVRNAMVISAVGLCLVLDEDDRAVRLALGSVGPTIIRAVEAE
                     AFAARAIAEAGAWESPAAALPDGVIDDVADLVARAARPIDDVRGTAAYRRHACRVLAR
                     RALTWSLEERATGAA"
     gene            1109..1579
                     /locus_tag="VF058_11755"
     CDS             1109..1579
                     /locus_tag="VF058_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6209016.1"
                     /translation="MLIRTNVNGEWREADAWAGSSLLSFLREGLGLPGSKNACEQGEC
                     GSCSVWLDGELVCSCLVLAAQAHEREVRTVEGLGSEDLLHPVQDVFLETGAVQCGFCT
                     PGLIVAVTDLLQRDPAPSEATVREALSGNLCRCTGYAKILDAVRLAAERMGGTA"
     gene            1576..2934
                     /locus_tag="VF058_11760"
     CDS             1576..2934
                     /locus_tag="VF058_11760"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015793324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HEX6209017.1"
                     /translation="MTRLLVEGCEVLVTMDEAGTEIRGGSLLIEDGVIAWVGSGEPPE
                     AKGARRVDGRGAVAMPGLVNTHHHLFQTLTRVRAQDEGLFEWLVELYPIWAGVDAEWE
                     LAAARVGLAELALSGCTTTTDHHYIFPKGADGFLEAEIEAAREVGLRFHPCRGSMDLG
                     ESDGGLPPDRVVENTDEILRETEEAIGRFHDPAPGAMLRIAVAPCSPFSSTERLMRES
                     AELARRHDVRLHTHIAETRDEERFCLERFGRRPLELLADLGFLGPDVWLAHCVHLDQK
                     DVRRMAESGTSAAWCPSSNLRLGSGTAPARSLLDEGANVGLGVDGSASNDAGHLLSEA
                     RQAMLTARGVGGPAAMSAREALRVATRGGAAALGRDDVGSLEVGKRGDAALFSVEGLG
                     FAGAGSDPVAALLFCSPRRVRDLLVDGHPVVENGRLTGVDEEEVARQGRRVSARALGG
                     RA"
     gene            2931..>3566
                     /locus_tag="VF058_11765"
     CDS             2931..>3566
                     /locus_tag="VF058_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6209018.1"
                     /translation="MSVRIGERLVRGVGESVRRVDGVPKVRGAFEFGSDLWAEGMLWG
                     HTVRTPHPHARVRSIDVSRAVAAAGVHAVLTSEDVPGHKMYGLEFQDQPVLAIDRVRY
                     QGEPVAAVAAEDPELARRAAALIRVEYEELPIVDDMELAMEPDAPKLHEWGNVLRHVR
                     IAHGDPEAEADVWVEGYYETGMQDQAPLGPEAGLAVPAEDGGVDLYVATQWL"
BASE COUNT          454 a         1212 c         1417 g          483 t
ORIGIN      
        1 cagaggtcct cacgtggccc gcgggccccg aggagatcga gaaggcgatg cgcctcgtcc
       61 ccgacgaggt ggtgcagatg atcacggcct cgggcacggc cgaggagtgc cgggcgaagg
      121 ttcgggagta cgtggaggcc ggctgcacgt gccccgtgct ctaccccctg ggcgacgacg
      181 tcggggcgat gctcgacgcc ttcgcggggg gagcgttcta gccgtggagg tcctgcggcc
      241 gcgcgacctc cgggaggccc tggcgatgaa ggcggcgcac ccggaggccc gccccatcgc
      301 gggggggacc gacctcatgg tcgagctgaa cttcgaccgg gaccggccgg aggcgatcct
      361 ggacctctgg ccggtgccgg agctccggga gcacgggcgg gaggacggga cgatcttcct
      421 cggagccggc gtcacgttcg cgcggatcat gcgcgagctg cccgagctcg tcccgctgta
      481 ccaggcgtcg cgaacggtgg gctccccgca gatccgcaac cgcggcacga tcggggggaa
      541 cctgggcacc gcctcgccgg ccggggacgc gctccccgtc ctggccgctt acgacgccga
      601 ggtcgtagtg gggagtgagg gggccgatcc gcgctccctc ccgtggagcg agttcctccg
      661 cggcccgaag cagaccacgc tgggcccgga ggagctgatc ctgggggtgc ggttccgtcc
      721 tccccgcggc ccggggagct tctcgaagat cggggtccgc aacgcgatgg tgatctcggc
      781 ggtcgggctc tgcctcgtgc tcgacgagga cgatcgcgcc gttcggctcg cgctgggctc
      841 ggtggggccc acgatcatcc gggcggtgga ggcggaggcg ttcgcggccc gggcgatcgc
      901 cgaggcgggc gcctgggagt ccccggcggc cgccctcccg gacggggtca tcgacgacgt
      961 cgccgacctg gtggcgcgcg cggctcgacc gatcgacgac gtgcgaggca ccgcggccta
     1021 ccgccgccac gcctgccgcg tgctcgctcg gcgggcgctc acctggagcc tggaggagcg
     1081 agcgaccggg gctgcgtgag gaggcgaggt gctcatcagg acgaacgtga acggggagtg
     1141 gcgcgaggcg gacgcctggg ccgggtccag cctcctctcc ttcctccgag aaggcctggg
     1201 gcttcccggc tcgaagaacg cgtgcgagca gggggagtgc ggttcctgct cggtctggct
     1261 ggacggcgag ctcgtctgct cgtgtctcgt gctcgcggcc caggcccacg agcgggaggt
     1321 gcggacggtc gaggggctcg gatcggagga cctcctccac ccggtccagg acgtgttcct
     1381 ggagacgggc gccgtgcagt gcggcttctg cacccccggg ctgatcgtgg ccgtcaccga
     1441 cctgctgcag cgggatcccg ccccgagcga ggcgaccgtc cgcgaggccc tctcggggaa
     1501 cctctgccgg tgcaccggct acgccaagat cctggacgcc gtgcgcctcg cggccgagcg
     1561 gatggggggc accgcgtgac gaggctcctg gtggagggct gcgaggtgct ggtcacgatg
     1621 gacgaggccg gcaccgagat ccggggcggc tcgctgctga tcgaggacgg cgtcatcgcc
     1681 tgggtggggt cgggggagcc tcccgaggcg aagggagccc ggcgcgtcga cgggcgcggg
     1741 gcggtggcga tgcccggcct ggtcaacacc caccatcacc tcttccagac gctcacccgg
     1801 gtccgggccc aggacgaggg gctcttcgaa tggctggtcg agctctaccc gatctgggcg
     1861 ggcgtggacg ccgaatggga gctggccgcg gcccgggtgg gcctggccga gctcgccctg
     1921 agcggatgca cgaccaccac cgaccaccac tacatcttcc cgaagggcgc cgacgggttc
     1981 ctggaagccg agatcgaggc ggcccgcgag gttggcctcc ggttccaccc gtgccggggg
     2041 tcgatggacc tgggggagtc ggacgggggg ctcccccccg accgagtggt ggagaacacc
     2101 gacgagatcc tgcgggagac cgaggaggcg atcggtcgct tccacgaccc cgcccctggg
     2161 gccatgctgc ggatcgcggt ggctccctgc tccccgttct cgtcgaccga gcggctgatg
     2221 cgcgagtcgg cggagctggc ccgtcgacac gacgtccggc tccacaccca catcgcggag
     2281 acccgggacg aggagcggtt ctgcctcgag cggttcggcc ggcgcccgct ggagctcctg
     2341 gccgacctcg gcttcctcgg ccccgacgtc tggctcgcgc actgcgtgca cctcgatcag
     2401 aaggacgtcc gcaggatggc cgagtccggc acgtcggcgg cctggtgccc gtcctcgaac
     2461 ctgcggctcg ggtccgggac cgcgccggcc cgctcgctcc tggacgaggg ggcgaacgtg
     2521 ggtctcgggg tggacgggtc ggcctccaac gacgccggcc acctgctgag cgaggctcgc
     2581 caggcgatgc tcaccgcgcg cggggtgggc ggccccgccg ccatgagcgc ccgcgaggcc
     2641 ctccgcgtgg cgacgcgcgg cggcgcggcg gccctgggcc gggacgacgt cgggtcgctg
     2701 gaggtcggca agcgcgggga cgcggccctc ttctcggtcg aagggctcgg gttcgcggga
     2761 gccggatcgg atccggtggc ggcgctgctc ttctgctcgc cccgccgcgt tcgggacctg
     2821 ctggtggacg gccaccccgt cgtcgagaac ggacgcctca ccggggtgga cgaggaggag
     2881 gtggctcgcc aggggcggcg ggtgtccgca cgagctttgg ggggacgggc atgagcgttc
     2941 ggatcgggga gcggttggtc cggggcgtcg gggagagcgt gcgtcgggtc gacggcgtcc
     3001 ccaaggtccg gggcgcgttc gagttcggca gcgacctgtg ggccgagggc atgctttggg
     3061 ggcacacggt ccggaccccg catccccatg cccgggtccg ctcgatcgac gtgtcccggg
     3121 cggtggccgc ggccggcgtg cacgccgtcc tcacctccga ggacgttccg ggccacaaga
     3181 tgtacggctt ggagttccag gaccagccgg tcctggcgat cgatcgggtg cgctaccagg
     3241 gggagccggt ggccgccgtg gccgccgagg acccggagct cgcgcgcagg gcggccgccc
     3301 tcatccgggt ggagtacgag gagctcccga tcgtggacga catggagctg gccatggagc
     3361 ccgacgcacc gaagctccac gagtggggga acgtcctgcg acacgtccgg atcgcgcacg
     3421 gcgacccgga ggccgaggcg gacgtctggg tggagggcta ctacgagacg gggatgcagg
     3481 accaggcgcc gctcgggccc gaagcagggc tggccgtccc cgcggaggac ggaggggtgg
     3541 atctgtacgt ggccacccag tggctc
//
LOCUS       DASUTW010000342         2342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1671093, whole genome shotgun sequence.
ACCESSION   DASUTW010000342 DASUTW010000000
VERSION     DASUTW010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671093"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..817
                     /locus_tag="VF058_11770"
     CDS             86..817
                     /locus_tag="VF058_11770"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6209019.1"
                     /translation="MTLVSDVMTTDVVSVEEDTPFKEIARLMHEHRISGVPVVDGRGH
                     LQGIVSEADVLTMEEEKVEPKRRRSFLEWFIDPKRLSEIEARAEDARAKDIMTSAVVT
                     VRPDTPIRRAITRLLDAGVKRLPVMDEDGKLVGIVSRTDLMSPLLRPDQEIEREVKED
                     VILRSMWIDPTMMVVEVEEGVVRLEGRVESRSVKEILIEMVRRVDGVVGVDADQLEYE
                     VDDRDVEAPPSRAGLQWGENWARRD"
     gene            complement(819..1601)
                     /locus_tag="VF058_11775"
     CDS             complement(819..1601)
                     /locus_tag="VF058_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005520452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6209020.1"
                     /translation="MELGLAGRKAVVTGGSKGLGRAIAEELASEGMDVAICSRTEDEV
                     RAAGEELRRRGVTVHAQAADVTDPAQVERFIAAAAEALGGIDVLVNNAGRAHPGTFAT
                     LTDEDWRDDLDVKLFSMIRCSREAIPHMRARGGGRIININAVYAKYPDPSFFATSTNR
                     AASVAFTKTLSMELAKERILVNSVNIGFVVTPQWENIHQKRAPELSREEFFARMAADE
                     VPLGRFGTPEEVSGIVAFLASERASYITGASIDVAGGMGRYL"
     gene            complement(1601..1996)
                     /locus_tag="VF058_11780"
     CDS             complement(1601..1996)
                     /locus_tag="VF058_11780"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6209021.1"
                     /translation="MTDADRYFTLDEDLQRAGEGRFLDLDGIEPAEFVPGLVFRPVLG
                     DRVMVNWVHFDPNTEAPKHAHEEEQITFVVEGEFEFDLDGDVRVMKPGMVAVIPAGVP
                     HGARTRDSSCLEIDVFVPPRKALLELTRD"
     gene            complement(1993..>2342)
                     /locus_tag="VF058_11785"
     CDS             complement(1993..>2342)
                     /locus_tag="VF058_11785"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6209022.1"
                     /translation="RSGGLEAQAERARELYGRRRRLMLEALEARLPEGIRWTRPRGGF
                     FTWLTYPQGVDTAALAKEAMERKVAFVPGAPFFPDGTGANTLRLSFSRVRDEEIDEGI
                     RRLAEVSAEGGKG"
BASE COUNT          356 a          792 c          843 g          351 t
ORIGIN      
        1 cggaccaagg aagaggccga cgcggccgag cactgagcgc acgcattgct ctaggcttgt
       61 cccgtccttc gatctggggg tgacgatgac gcttgtgtcc gacgtgatga cgaccgacgt
      121 ggtgtccgtg gaggaggaca cacccttcaa ggagatcgcc cgactgatgc acgagcaccg
      181 gatcagcggc gtgccggtcg tcgacgggag ggggcacctg caggggatcg tgagcgaggc
      241 cgacgtgctg accatggagg aggagaaggt cgagcccaag cgtcggcgga gcttcctgga
      301 gtggttcatc gacccgaagc gcctctcgga gatcgaggcc cgggcagagg acgcccgagc
      361 gaaggacatc atgaccagcg ccgtggtgac cgtgcgcccc gacacgccga tcaggcgagc
      421 catcacgcgc ctgctcgacg cgggcgtcaa gcgcctcccg gtgatggacg aggacgggaa
      481 gctcgtgggc atcgtcagcc ggaccgacct catgtcgccg ctgctccgcc ccgaccagga
      541 gatcgagcgc gaggtgaagg aggacgtgat cctgcggtcg atgtggatcg acccgacgat
      601 gatggtggtc gaggtggagg agggggtcgt tcgactcgag ggacgcgtcg agagccgcag
      661 cgtgaaggag atcctgatcg agatggtgcg cagggtcgac ggggtcgtcg gcgtggacgc
      721 cgaccagctc gagtacgagg tggatgatcg ggacgtcgag gccccgccct cccgggccgg
      781 cctccagtgg ggcgagaact gggcccggcg ggattgacct agaggtaccg ccccatgccg
      841 ccggcgacgt cgatgctggc gccggtgatg tagctcgcgc gctcgctcgc caggaaggct
      901 acgatccccg agacctcctc gggggtcccg aacctcccca gcgggacctc gtccgccgcc
      961 atccgggcga agaactcctc ccgggagagt tccggcgcgc gcttctggtg gatgttctcc
     1021 cactgcggcg tgacgacgaa cccgatgttc acactgttga cgaggattcg ctccttcgcc
     1081 agctccatgg agagggtctt ggtgaacgcg acgctggccg cgcggttggt ggaggtggcg
     1141 aagaacgagg ggtcggggta cttggcgtag accgcgttga tgttgatgat gcggccgccg
     1201 ccgcgggccc gcatgtgggg gatcgcctca cgtgagcagc ggatcatcga gaagagcttg
     1261 acgtcgaggt cgtccctcca gtcctcatcg gtgagggtcg cgaaggtccc ggggtgcgcg
     1321 cggccggcgt tgttgaccag gacgtcgatc ccgccgagcg cctccgctgc cgcggcgatg
     1381 aaccgctcga cctgcgcggg gtcggtgacg tcggcggcct gggcgtggac cgtgacgccg
     1441 cggcggcgga gctcctcgcc ggccgcgcgc acctcgtcct cggtccgcga gcagatcgcg
     1501 acgtccatcc cctcggacgc gagctcctcg gcgatggcgc ggccgagccc cttcgagccg
     1561 ccggtgacga cggccttcct gccggcgagc cccagctcca tcagtcgcgg gtcagctcga
     1621 ggagggcctt ccggggcggg acgaagacgt cgatctccag gcaggagctg tctctggtcc
     1681 tcgcgccgtg ggggacgccg gccgggatca ccgcgaccat cccgggcttc atcaccctga
     1741 cgtcgccgtc gagatcgaat tcgaactcgc cctccaccac gaaggtgatc tgctcctcct
     1801 cgtgcgcgtg cttcggggcc tcggtgttcg ggtcgaagtg gacccagttc accatgacgc
     1861 ggtcgccgag cacgggccgg aagaccagcc cgggcacgaa ctccgccggc tcgatcccgt
     1921 ccaggtcgag gaagcggccc tcgcccgcgc gctgcaggtc ctcgtccagc gtgaagtagc
     1981 ggtcggcgtc cgtcatccct tccctccctc ggccgaaacc tcggccagcc tgcggatccc
     2041 ctcatcgatc tcctcgtccc gaacccggct gaacgacagc cggagcgtgt tggcgccggt
     2101 cccgtcgggg aagaacggcg cccccggcac gaaggccacc ttccgctcca tcgcctcctt
     2161 cgccagcgcg gcggtgtcca cgccctgggg gtaggtcagc caggtgaaga acccccctcg
     2221 ggggcgcgtc cagcggatgc cctcggggag gcgcgcctcc agcgcctcga gcatgagccg
     2281 gcgccgcctc ccgtagagct cgcgggcgcg ctcggcctgc gcctccagac cgccggagcg
     2341 ac
//
LOCUS       DASUTW010000343         7576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_376570, whole genome shotgun sequence.
ACCESSION   DASUTW010000343 DASUTW010000000
VERSION     DASUTW010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7576
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376570"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VF058_11790"
     CDS             <1..568
                     /locus_tag="VF058_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6209023.1"
                     /translation="TMLAGRIRHGGSLLAPFAVAYVVAGEGDLPPEARLRLDAQLDLL
                     PGARAGGLVLYRNTRAMPLAGILPAAPPDGDVRAVDILAPARVPRLGMAPFRRVEPNT
                     WTAAPDLEETGTAFVSKEYEPAWVLRAEDGREYEAFRAFGWAVGVEAPPRSGRLTLDR
                     REGWVRTAEVTGLLVVWVGALWILRRRG"
     gene            565..1926
                     /locus_tag="VF058_11795"
     CDS             565..1926
                     /locus_tag="VF058_11795"
                     /inference="COORDINATES: protein motif:HMM:NF040160.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5719 family protein"
                     /protein_id="HEX6209024.1"
                     /translation="MSRPWVERLRLPGALLVSVLVVAGGLALERGLGARTRTPGEVPS
                     AVSGAWFCPHGGSGRWRVWLVVANPTQEPAQIRATTYGGERPESQVATVDPGTQRYLE
                     VPAADPGAASAVEFFGGPVAAGTVMTRSTGKGEPLGRAAEPCLAESAARWYLPEGTTL
                     RGFDQRVVVMNPFPQEAVVSLWLTDERETIKPGDLQGIVLPGRRSASFDIGAFSLGNQ
                     TLAVTVDVRLGKVAVGAVGLSETGIRASTGVTAPAGTWVMPGALDDEPSDLTVLGTSR
                     AEAPFRARVQGAEGQIEVISEAVVPPGKAETHAVPAQGAGLVAVADGSEPFVAGRRLN
                     TPGDLASVPAIAEGAASWIALPTTTPTGGASTLFIQNAGEQVADVRIVLVTETGPAEA
                     PEIGRIALRPGGYRAVDLSQLVGDEPVSVVVRAESGTVIVGQASRDPGGYAVASGAPL
                     DLP"
     gene            1968..3029
                     /locus_tag="VF058_11800"
     CDS             1968..3029
                     /locus_tag="VF058_11800"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEX6209025.1"
                     /translation="MRGRRHVASGRRLLRVVVFTLVGLMVLGGGVAYGAYRFDRAQTG
                     KILPGVTIAGVDVGEMTRDEAIAAVREAIESDLSQDIEVRARDRSWHVTPAELGTRAD
                     IEEKVDRALAVGEGMAWHERALARILDRPVEEDLRVRLRRDGETIQRFLGIVSEATYA
                     APNDASFGIEDGELVLTRPKMGRELDVKRSRERLRSALREGNPLVTLPVSKVEPDVSP
                     ESLGLSIVVRISENRLYLYDGFKVIKRYDVATGTGGYPTPQGTWNIWDKRENPVWVNP
                     APDGWGADLPAVIGPGPGNPLGTHALYLDAPGIRIHGTYAEDTIGTFASHGCIRMRIA
                     ESKELFSLIPIGTKVHILP"
     gene            3303..3533
                     /locus_tag="VF058_11805"
     CDS             3303..3533
                     /locus_tag="VF058_11805"
                     /inference="COORDINATES: protein motif:HMM:NF023430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 family protein"
                     /protein_id="HEX6209026.1"
                     /translation="MRRCAKMRCEEVAAASVAVRYSDRTAIVEPLSAERDPNLLDLCE
                     PHASSLTAPRGWRLVDQRPGAMELPSPVHASA"
     gene            3567..3857
                     /locus_tag="VF058_11810"
     CDS             3567..3857
                     /locus_tag="VF058_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006885937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6209027.1"
                     /translation="MLEHDLIRRIRALDEYDLRRLMIFVRGLLVARRNDDFQVEEPAR
                     AGKVTYRQELVRCGRESCGRCPHGPYWYAYWREDGRLRSRYIGKDLPEDRPR"
     gene            3999..4205
                     /locus_tag="VF058_11815"
     CDS             3999..4205
                     /locus_tag="VF058_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6209028.1"
                     /translation="MSAIKSKDHTAPLTTCPSCGSGEVVVVTFGVGDSLIVFRTCPPC
                     EARWWEQDGDPIDRETAIPLVTAA"
     gene            4240..5574
                     /gene="manB"
                     /locus_tag="VF058_11820"
     CDS             4240..5574
                     /gene="manB"
                     /locus_tag="VF058_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222900.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX6209029.1"
                     /translation="MAPPEIFKAYDIRGIYPDQLDEGTSRRIGAAFASFTAAERVVVG
                     RDCRISSPGLSAAFIEGVVGRGADVVDIGLATTDMLYFASGSLDLPGAMFTASHNPPE
                     YNGLKLCREGAAPIGEETGLREIRDLAEREPAPSGAPGSARERSMLDAYVEHVVGFVD
                     VSRLAPLTVVADAANGMAGLVLEPIFERLPAKLIGLYLDLDGTFPNHPADPIQPENQE
                     DLRKAVVEHDADVGLAFDGDADRVFVVDEGANGVSGSLLVALIAEKMLEREPGATILH
                     NVICSWVVPEVIRENGGVPVRTRVGHSFIKQVMAETGAIFGGEHSGHYYFRDHYRADS
                     GLVAALLVLEQMSEAGVPLSELLRPFRRYHASGEINSEVEDQDAAIDGLAQRYSDGEQ
                     DRLDGLTVEYEDWWFNARPSNTEPLLRLNVEARHEGLLAERTAELLAIIRGE"
     gene            5576..5758
                     /locus_tag="VF058_11825"
     CDS             5576..5758
                     /locus_tag="VF058_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX6209030.1"
                     /translation="MALDQELLDVLACPNCRGEVEYREEEGFIVCLGKCGYKYPVRDD
                     IPVMLIEEAVKPSGGG"
     gene            5764..6813
                     /locus_tag="VF058_11830"
     CDS             5764..6813
                     /locus_tag="VF058_11830"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX6209031.1"
                     /translation="MRSPIDPSTVAAGDPSGMLEAFLRLPEQLVVGYEAGRSAGLAPA
                     EPRSLIICGMGGSAAGGDVVASAFADRLRPPVASVRGYSVPAWCGPEDLVLCLSFSGG
                     TEETVACLEEARRRGSTVTAVASGGRLIELAGEGAVTIPAAAPMPRAGLSLLAGGALG
                     ALVAMGLLPDVDAEVKEAASSLAELSEELAPGRDRNEAMEVARAVGSQHVPVIWGSEG
                     LAEPAAFRWKAAFHENAKIPALSSALPELDHHEIAGWSEGRGHGFLVIALRHPGEHGS
                     VGPRLEATFEAIEESGIGWREAHARGRGPLAHSLSLILLGDVASVYHALARGVDPGPI
                     EAIDRLKRRLAEGRR"
     gene            6810..>7576
                     /locus_tag="VF058_11835"
     CDS             6810..>7576
                     /locus_tag="VF058_11835"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX6209032.1"
                     /translation="MMVGPGDDLAERGAEAVRAVTDLRPRVALIAGSGLGDALDAIEP
                     DAELAFSDLPGFPLPSVPGHAGRLVVGTLEEVPVAGFLGRVHFYEGHPMSLVTLPARL
                     AAALGAGTLVVTASTGGLDPDLEPGSLVIASDHLNLLGENPLRGWRDEEGRPVFVDLT
                     RVYPPEPAEAAERAAREVGLPFARGVYAAMPGPTYETPSEAEMLRRLGGDVVGMSVVP
                     EACAAAALGMRCLGLFCVTNRVGVHVSHEEVTRVAAA"
BASE COUNT          999 a         2594 c         2878 g         1105 t
ORIGIN      
        1 cacgatgctc gccggccgca tccgccacgg cggctcgctg ctcgcgcctt tcgccgtggc
       61 ctacgtggtg gcgggcgagg gcgacctccc gcccgaggcc cggctcaggc tggacgcgca
      121 gctcgacctg ctgccggggg cgcgtgcggg cgggctcgtc ctgtaccgga acacgagggc
      181 catgcccctg gcggggatcc tccccgccgc cccgcccgac ggcgacgtgc gggcggtgga
      241 catcctcgcg ccggcgcggg tcccgcggct ggggatggcg ccgttccggc gcgtggagcc
      301 gaacacgtgg accgcggccc cggacctcga ggagaccggc acggccttcg tctccaagga
      361 gtacgagccg gcctgggtcc ttcgtgccga ggacgggcgg gaatacgagg cgttccgcgc
      421 cttcggatgg gcggtcggcg tcgaggcgcc gccccgatcg ggtcggttga cgctggaccg
      481 tcgggagggg tgggtccgca cggccgaggt gaccgggctc ctcgtggtct gggtcggagc
      541 gctctggatc ctccggaggc gaggatgagc cgtccctggg tggagcggct gcggctcccg
      601 ggggcgctgc tcgtgtcggt cctcgtcgtg gccgggggcc tggctctgga gcgagggctc
      661 ggggcccgga cccggacgcc aggggaggtg ccctccgcgg tctcgggggc ctggttctgt
      721 ccgcacgggg ggagcggccg gtggcgcgtg tggctggtcg tggccaaccc gacccaggag
      781 ccggcccaga tccgggccac gacgtacggg ggcgagcgcc ccgagtccca ggtcgccacc
      841 gtcgacccgg ggacccagcg gtacctcgag gtcccggcgg ccgaccccgg agccgcgtcg
      901 gccgtcgagt tcttcggcgg ccccgtcgcc gcggggaccg tgatgacgcg gagcactggc
      961 aagggcgagc cgctgggccg cgccgccgag ccctgtttgg cggagtcggc cgcccgctgg
     1021 tatctcccgg agggtacgac cctgcggggc ttcgaccagc gcgtggtggt gatgaacccc
     1081 ttcccgcagg aggcggtggt cagcctgtgg ctcaccgacg agcgggagac gatcaagccg
     1141 ggagacctcc agggcatcgt gctgccgggc cgccggtccg cctcgttcga catcggggcc
     1201 ttctcgctcg ggaaccagac gctggccgtg accgtggacg tccgtctggg gaaggtcgcg
     1261 gtcggcgcgg tgggcctgag cgagacgggg atccgggcgt cgaccggggt cacggcgccg
     1321 gccgggacgt gggtgatgcc gggcgcgctg gacgacgagc cgagcgacct gaccgtcctg
     1381 ggaacgagcc gcgcggaggc ccccttccgg gcccgcgtcc agggggcgga aggccagatc
     1441 gaggtgatct ccgaggccgt ggtccctccg gggaaggccg agactcacgc cgtgccggcc
     1501 cagggggcgg ggctcgtcgc ggtcgcggac gggtccgagc ccttcgtggc cgggcggagg
     1561 ctgaacaccc ccggcgacct cgccagcgtc cccgccatcg cggagggagc ggcgtcgtgg
     1621 atcgcgctcc ccaccaccac tcccacgggg ggcgcatcga ccctcttcat ccagaacgcc
     1681 ggagagcagg tcgcggatgt ccggatcgtc ctggtgacgg agacgggtcc ggcggaggct
     1741 cccgagatcg ggcggatcgc cctccgaccg gggggctacc gcgcggtcga tctctcgcag
     1801 ctcgtcgggg acgagccggt ctcggtggtg gtgcgggccg aatcggggac cgtgatcgtc
     1861 ggccaggcct ctcgggaccc cggggggtac gccgtcgcct ccggcgcccc gctcgatctg
     1921 ccctgaacgc cctgttcgga agggaggctg cacggtatca tccgtctatg cgaggtcgac
     1981 gacacgtcgc ttcgggacga cgcctgctcc gcgtcgtcgt gttcacgctg gtcggtctca
     2041 tggtgctcgg cgggggggtc gcatacgggg cgtatcggtt cgatcgcgct cagaccggga
     2101 agatccttcc gggtgtgacg atcgccggcg tcgacgtcgg agagatgacg cgcgacgagg
     2161 cgatcgcggc ggttcgagag gcgatcgagt ccgatctctc ccaggacatc gaggtgcgag
     2221 cccgcgaccg ctcctggcac gtgacgccgg ccgagctcgg gaccagggcc gacatcgagg
     2281 agaaggtgga tcgagcgctc gccgtggggg aggggatggc ctggcacgaa cgggccctcg
     2341 ccaggatcct cgaccgtccg gtggaggagg atctccgcgt ccggctccgg agggacggcg
     2401 agacgattca gcgcttcctc gggatcgtga gcgaggccac gtacgcggcg ccgaacgacg
     2461 cctcgttcgg catcgaggac ggggagctgg tcctcacccg accgaagatg gggcgggagc
     2521 tcgacgtgaa gcgctcccgc gagaggctgc gctccgcgct gcgagagggt aaccccctgg
     2581 tgacgctccc ggtctccaag gtcgagccgg acgtcagccc cgagagcctc gggctctcga
     2641 tcgtcgtgcg gatctccgag aatcgcctgt acctctacga cggcttcaag gtgatcaagc
     2701 gatacgacgt cgccaccggc acgggcgggt accccacgcc ccagggcacc tggaacatct
     2761 gggacaagcg ggagaacccc gtgtgggtga acccggctcc cgacggctgg ggcgccgatc
     2821 tccccgccgt catcggcccg ggccccggga acccgctcgg cacgcacgcg ctctacctcg
     2881 acgcgccggg catccggatc cacggcacct acgccgagga cacgatcggg accttcgcct
     2941 cgcacggctg catccgcatg cggatcgccg agtcgaagga gctcttcagc ttgatcccga
     3001 tcgggaccaa ggtgcacatc cttccgtaga aagagtgaca agacccgatg ccggggttgc
     3061 gtcgcccctg agctcgggtg tagggtggtg gagtactccg aagagacagg acggggcaac
     3121 ccggacggtc gcttcggagt acgctttttc gtggcaggga gccttcgggg cccctgcctc
     3181 gcctttttcc ccggccccgc gatcgcgtgg gccggcgcat cctgtctccg ttcttaggta
     3241 acgaatccgt tacctaagaa gggtccttgt gaacgacatc gatgtggttt accctccgct
     3301 ctatgcgacg ctgcgccaag atgcgctgtg aggaggtcgc ggccgcgtcg gtcgccgtcc
     3361 gctacagcga ccgaaccgcg atcgtcgagc ccctctcggc ggagcgtgac cccaacctgc
     3421 tcgatctctg cgagccgcac gcctcgagcc tcacggcccc gaggggctgg cgcctcgtgg
     3481 atcagcgccc cggggccatg gagctcccga gcccggtcca cgcctcggcc tgatccgcgg
     3541 gtcttgggta cacttccgta cccaagatgc tcgagcacga cctgatccga cggatccgcg
     3601 cgctggacga gtacgacctc cggcgcctga tgatattcgt ccgcgggctg ctggtggccc
     3661 gcaggaacga cgatttccag gtcgaggagc ccgcccgggc cggcaaggtg acctaccgcc
     3721 aggagctcgt ccgctgtggc cgagagagct gtggccggtg tccccacggg ccctactggt
     3781 acgcctactg gcgggaggac ggtcggctcc ggtctcgcta catcgggaag gacctgcccg
     3841 aggaccggcc gcgctgacgc ggagcgcgtc ggcactgtgg gccgggcggg ggacacgaag
     3901 ctgatccatc gaggccctcc ccgcccacgc ccggggtgcg ggggaaatga ccccttcgtg
     3961 ctatggaggg ggaggcgccc gggcgccgat ggctgggtat gagcgcgatc aagagcaagg
     4021 accacacggc gcctctgacc acgtgcccca gctgcggatc cggtgaggtc gtcgtcgtga
     4081 cgttcggggt cggtgactcg ctgatcgtct tccggacctg ccctccctgt gaggcgagat
     4141 ggtgggagca ggacggggac ccgatcgacc gcgagaccgc catcccgctc gtcaccgccg
     4201 cctgaggccc tccccggcgc ctgggataga atccgcggca tggcgccgcc ggagatcttc
     4261 aaggcgtacg acatccgcgg catctacccg gaccagctgg acgagggcac gtcccggcgc
     4321 atcggcgcgg ccttcgcctc gttcaccgcc gcggagcgcg tcgtggtcgg cagggactgc
     4381 aggatctctt cccccggact ctcggccgcc ttcatcgagg gggtcgtggg gcgcggggcc
     4441 gacgtcgtcg acatcgggct cgccaccacg gacatgctct acttcgcgtc tggctccctc
     4501 gatcttcccg gagcgatgtt caccgccagc cacaaccccc ccgagtacaa cgggctgaag
     4561 ctgtgccggg agggggcggc tccgatcggg gaggagaccg ggcttcgcga gatccgcgat
     4621 ctcgccgagc gcgagccggc cccctcggga gccccgggct ccgcccggga gcgatcgatg
     4681 ctcgacgcgt acgtggagca cgtcgtgggc ttcgtggacg tgtcgaggct cgccccgctc
     4741 accgtggtgg ccgacgccgc caacgggatg gccgggctgg tcctcgagcc gatcttcgag
     4801 cgcctgccgg cgaagctgat cggcctctac ctcgacctcg acggcacctt ccccaaccac
     4861 cccgccgatc cgatccagcc cgagaaccag gaggacctgc ggaaggcggt ggtcgagcac
     4921 gacgcggacg ttgggctcgc cttcgacgga gacgccgacc gggtgttcgt cgtggacgag
     4981 ggcgcgaacg gggtctccgg gtcgctcctc gtggcgctga tcgccgagaa gatgctggag
     5041 cgagagcccg gggcgacgat cctgcacaac gtgatctgct cgtgggtcgt acccgaggtg
     5101 atccgggaga acggcggcgt cccggtccgc acgcgggtcg gccactcgtt catcaagcag
     5161 gtgatggccg agaccggagc catcttcggc ggcgagcact cgggccacta ctacttccgt
     5221 gaccactacc gggccgactc ggggctcgtg gccgcgctcc tggtgctgga acagatgtcc
     5281 gaggcgggcg tccccctctc ggagctgctg cggccgttcc gccggtacca tgccagcggc
     5341 gagatcaact ccgaggtcga ggaccaggac gcggcgatcg acggtctcgc ccagcgctac
     5401 tcggacggcg agcaggaccg gctcgacggg ctcacggtcg agtacgagga ctggtggttc
     5461 aacgcgcggc cgtccaacac cgagccgctg cttcgcctga acgtcgaagc ccgtcacgag
     5521 gggctcctcg cggagaggac cgcggagctc ctcgcgatca tcagagggga gtgagatggc
     5581 gctggatcag gagctgctgg acgtcctggc gtgccccaac tgccggggcg aggtcgagta
     5641 ccgggaggag gaggggttca tcgtctgcct ggggaagtgc ggctacaagt acccggtgcg
     5701 cgacgacatc ccggtgatgc tgatcgagga ggcggtgaag ccctcggggg gcggctaggc
     5761 cggatgcgat ccccgatcga tccctccacc gtcgcggccg gcgacccgag cggcatgctc
     5821 gaggcgttcc tgcggctccc cgagcagctc gtcgtggggt acgaggccgg ccggtcggcc
     5881 gggcttgccc cggccgagcc ccgctcgctg atcatctgtg ggatgggggg ctccgcggcg
     5941 gggggcgacg tcgtggcgtc ggccttcgcc gaccggctcc ggcccccggt cgcgtcggtg
     6001 cggggctact ccgtgcccgc ctggtgcggc cccgaggacc tcgtcctgtg cctctcgttc
     6061 tccgggggga cggaggagac ggtggcctgc ctcgaggagg cgcggcggcg gggatccacg
     6121 gtgacggcgg tcgcctccgg tgggcgtctg atcgagctgg ccggcgaggg cgccgtcacg
     6181 atccccgccg ccgcgcctat gcctcgggcg ggcctcagcc tgctggcggg cggggcgctg
     6241 ggcgccctcg tggcgatggg cctcctcccc gatgtggacg cggaggtgaa ggaggccgcc
     6301 agcagcctcg ccgagctgtc cgaggagctg gcccccggcc gcgaccggaa cgaggcgatg
     6361 gaggtggccc gcgcggtcgg ctcccagcac gtgccggtca tctggggatc ggaggggctg
     6421 gcggagccgg cggccttccg atggaaggcg gcgttccacg agaacgcgaa gatcccggcc
     6481 ctgtcctcgg cgctccccga gctcgaccat cacgagatcg ccggctggtc ggaggggcgg
     6541 ggccacggct tcctggtgat cgccctgcgc catcccgggg agcacgggag cgtgggcccc
     6601 aggctcgagg cgaccttcga ggcgatcgag gagtcgggga tcggctggcg agaggcccac
     6661 gcccggggac gcggcccgct cgcgcattcg ctctcgctga tcctgctggg cgacgtggcg
     6721 agcgtttacc acgccctggc gcggggcgtg gatcccgggc cgatcgaggc gatcgatcgg
     6781 ctgaagcggc gcctggccga ggggcggaga tgatggtcgg ccccggggac gatctggccg
     6841 agcggggagc cgaggccgtc cgggccgtga ccgacctccg cccgcgcgtg gcgctgatcg
     6901 cgggatcggg gctgggcgat gcgctggacg cgatcgagcc cgacgccgag ctcgccttct
     6961 cggacctgcc ggggttcccc ctgccctcgg tgccgggaca cgccggccgc ctggtcgtgg
     7021 gcacgctcga ggaggtcccc gtggccggct tcctgggacg ggtgcacttc tacgaggggc
     7081 atccgatgtc gctcgtcacg ctgcccgctc ggctcgcggc cgcgctcggc gcgggaacgc
     7141 tcgtcgtgac cgcctccacc ggcgggctcg accccgacct ggagccgggg tcgctcgtca
     7201 tcgcctcgga tcacctgaac ctcctgggcg agaaccccct ccgggggtgg cgcgacgagg
     7261 agggtcggcc ggtgttcgtg gacctgacgc gcgtgtaccc tccggagcct gcggaggccg
     7321 ccgagcgggc cgcccgtgag gtggggctcc cgttcgccag gggcgtgtac gctgcgatgc
     7381 cgggtcccac ctacgagacg ccgtcggagg ccgagatgct gcggcggctg ggcggcgacg
     7441 tcgtggggat gtcggtggtt cccgaggcgt gcgccgcggc ggcgctgggg atgcggtgtc
     7501 tgggcctctt ctgcgtcacg aaccgcgtgg gggtccacgt gagccacgag gaggtgacgc
     7561 gggtggcggc ggcgtt
//
LOCUS       DASUTW010000344         4342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_638970, whole genome shotgun sequence.
ACCESSION   DASUTW010000344 DASUTW010000000
VERSION     DASUTW010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638970"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VF058_11840"
     CDS             <1..201
                     /locus_tag="VF058_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX6209033.1"
                     /translation="ELQAEVQPGNSGGPFVTADGDVAGVVFAASVGNDDVGYALTSTE
                     AMPRIERGIGQTEPTDTGPCLQ"
     gene            complement(214..888)
                     /locus_tag="VF058_11845"
     CDS             complement(214..888)
                     /locus_tag="VF058_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6209034.1"
                     /translation="MPVIFPEGTRAELVYPSHLRLEELAVNPDTFATGGPKVCGYPVH
                     ATRHDPHDGWVRGEEPLAEHASPDGTTVALWEGRPDHRPYNYLVYHFGSWAVLVPCRG
                     HVSSEALAVWAESLQGEESAEGLLVLDGVAPLVLHPWRDQHGPTLRLSDEDVIVDVRP
                     LSEQCDPSSGWGGDTDPRDGVIQWCVQSAGSIYVYAVGFTPAGKGFLQALVDGLQVRR
                     VEPAAS"
     gene            complement(1140..3353)
                     /locus_tag="VF058_11850"
     CDS             complement(1140..3353)
                     /locus_tag="VF058_11850"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX6209035.1"
                     /translation="MTSGPELPDSPRFQDALERILNEGAPEIVTDLPGKVLATKEGPT
                     FLYADSEGHLDDRQELGLGFYHLDTRFLSYFRFKLSGRDPILLSSSAERAYMAHIDMT
                     NPALYDADGRVAAAQQSLNVRRIRAVGDRLFEKTRIKNYNARTVEVDVTYTFGADFAD
                     IFEVRGLRRERRGHHEPPKAEGRSISFSYVGEDDVQRTTRIAFGAEPDRMEVRGDVVE
                     ATFGLRIPSGETRVLSLQVEPIVGPGRELPERDYDPTIHELRRSYESWERESTRIRAD
                     NELFNELLRRGLRDLRALWMSADGGATLAAGIPWYVSPFGRDALITSHQLLMVNPQLA
                     RDTLTLLASLQARDRDDWRDAEPGKILHEMRRGELAGAGLIPHTPYYGSVDSTPWFLI
                     LLAQYHRWTADTELVRELMPAAEAALAWIDDHGDLDGDGFVEYLRRSDRGLENQGWKD
                     SWNSVVHRDGTLAKPPIGLVEVQAYVYMAKVRMAGLFASLGDHARAVALRDEATVLRK
                     RFNDEFWMEDEAYFAEALDGKKRQVRAITSNPGHALYCDAVEPAKAEAIAQRLFAPDM
                     FSGWGIRTLSKSERAYNPMSYHNGSVWPHDNALIAAGLKRYGFVEATNRVATAMFEAA
                     ITMDYMRLPELFCGFVRRSPNRPVQYPVACSPQAWAAGAPFLLLQAMLGVSARADENL
                     LTINKPHLPRWLNEVELRNLRVGKSTLSLTFRREGEVTAFTLASKEGDVRVVMEE"
     gene            complement(3396..>4342)
                     /gene="thrB"
                     /locus_tag="VF058_11855"
     CDS             complement(3396..>4342)
                     /gene="thrB"
                     /locus_tag="VF058_11855"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX6209036.1"
                     /translation="RRSRPARLAWRPTPARSPPPWGSRGMRLTVRVPATAANLGPGFD
                     ALALALGLHNEVVCETSAEPAVEVSGEGAGELPEDASNLIFRSMAFVAREAGGSLPSL
                     RLRCRNEIPLERGLGSSASAVVAGVLLADRLLGAGLDPDRLLGLAVDVEGHPDNVAAC
                     LRGGGVIAYLSRDGWRAERLEPHPDLAPVVLIPEGERVPTQDARRDLPLDVPLRDAAF
                     NLGRSALAVRALTHDPALLHEALEDRLHQARRLELAPGARALFRDLRDAEIPACVAGA
                     GPTILAFEGLGGPIPELGPGWRVERPGVPSGGASIAEA"
BASE COUNT          649 a         1520 c         1581 g          592 t
ORIGIN      
        1 gagctccagg ccgaggtcca gccggggaac tcgggaggcc cgttcgtgac ggccgacggc
       61 gacgtggcgg gggtggtctt cgcggcctcg gtggggaacg acgacgtagg ctacgcactc
      121 acctccaccg aggcgatgcc ccggatcgag cgggggatcg gccagaccga gcccaccgac
      181 accggccctt gcctccagta gccgtccggt cgctcagctg gcggctggct cgacgcggcg
      241 cacctggagc ccgtccacga gggcctgcag gaaccccttt cccgcaggag tgaagccgac
      301 cgcatagacg tagatcgagc cggccgactg cacgcaccac tggatcacgc cgtcccgcgg
      361 atccgtgtct ccgccccagc cggacgaggg atcgcactgc tcggagagag gccggacatc
      421 gacgatcaca tcttcgtcgg agaggcgaag cgttgggccg tgctgatcgc gccacggatg
      481 gaggacgagc ggcgcaactc cgtcgagcac gaggagtccc tcagccgact cttcgccctg
      541 caagctctcg gcccagacgg cgagtgcttc cgagctcacg tgacctcggc acggaaccag
      601 gacggcccag gagccgaagt ggtagacgag atagttgtag ggacggtggt cgggccgccc
      661 ctcccacaaa gccaccgtgg tcccgtcggg acttgcgtgc tccgcgagcg gctcctcgcc
      721 cctgacccat ccgtcgtggg gatcgtggcg tgtcgcgtgc accggataac cgcacacctt
      781 cggaccgccg gtggcgaacg tatccgggtt gacggcgagc tcttcgagtc gaaggtgcga
      841 tggataaacg agctccgccc tggtgccctc ggggaagatc accggcatga ccgtccgatc
      901 gccggcagca tacgtgggcg gagcccagcg ttcccctgcc ggcaggtagc ctggcgccgg
      961 tgatggactt ccctgctggg cggtcggggc cgccagagga cgaggggact ccagagctcc
     1021 ttagcagccg gcaacggcca tcagcgccag caggaccggg ctcatccgga ggagcgatcc
     1081 cataccggtc agacgcttcg aaccgaccag cggttcgatg gccgatggag gatccccccc
     1141 tactcctcca tgacgacgcg gacgtcgccc tccttggagg ccagcgtgaa ggcggtgacc
     1201 tcgccctcgc ggcggaacgt cagcgagagc gtgctcttcc ccacgcggag gttccgcagc
     1261 tcgacctcgt tcagccaccg cgggagatgc ggcttgttga tcgtgagcag gttctcgtcg
     1321 gcccgagccg agaccccgag catcgcctgg aggaggagga acggggcgcc ggccgcccag
     1381 gcctgcggcg agcacgccac cgggtactgg acgggccggt tcggcgaccg ccgcacgaac
     1441 ccgcagaaga gctcgggcag gcgcatgtag tccatcgtga tcgcggcctc gaacatcgcg
     1501 gtggctaccc ggttcgtggc ctccacgaaa ccgtagcgct tcagccccgc cgcgatcagc
     1561 gcgttgtcgt gaggccagac cgagccgttg tggtagctca tcgggttgta ggcccgctcc
     1621 gacttgctca gcgtccggat cccccacccc gagaacatgt cgggggcgaa gagccgctgg
     1681 gcgatggcct cggccttcgc cggctccacg gcgtcgcagt agagggcgtg ccccgggttc
     1741 gacgtgatgg cccgcacctg gcgcttcttc ccgtcgagcg cctcggcgaa gtacgcctcg
     1801 tcctccatcc agaactcgtc gttgaatcgc ttccggagga cggtggcctc gtcgcggaga
     1861 gcgaccgccc gggcgtggtc gccgagcgag gcgaagaggc cggccatccg gaccttcgcc
     1921 atgtacacgt aggcctgcac ctcgacgagg ccgatcgggg gtttggccag cgtcccgtcg
     1981 cggtgcacga ccgagttcca cgagtccttc cagccctggt tctccaggcc ccggtccgag
     2041 cggcgcaggt actcgacgaa gccgtccccg tccaggtcgc cgtggtcgtc gatccaggcc
     2101 agcgcggcct cggccgccgg catcagctcg cggacgagct cggtgtcggc cgtccatcgg
     2161 tggtactggg ccaggaggat caggaaccac ggcgtcgagt ccaccgagcc gtagtagggc
     2221 gtgtgcggga tcaggccggc cccggccagc tcgccccgac gcatctcgtg gaggatcttc
     2281 ccgggctccg cgtcccgcca gtcgtcccgg tcccgggcct ggagcgacgc cagcagcgtc
     2341 agcgtgtcgc gggccagctg cggattcacc atcaggagct ggtgcgaggt gatgagcgcg
     2401 tcgcggccga acggcgagac gtaccagggg atgccggccg ccagggtcgc cccgccgtcg
     2461 gcgctcatcc agagggctcg caggtcccgg agcccgcgac ggagcagctc gttgaagagc
     2521 tcgttgtcgg cgcggatcct ggtggactca cgctcccacg actcgtagga gcgccgcagc
     2581 tcgtggatcg tggggtcgta gtcgcgctcc ggcagctcgc gacccggtcc cacgatcggc
     2641 tcgacctgca gggacaggac ccgggtctcg cccgacggga tgcggagccc gaacgtggcc
     2701 tccacgacgt cgccccgcac ctccatccgg tcgggctcgg ccccgaacgc gatgcgcgtc
     2761 gtcctctgca cgtcgtcctc ccctacgtac gagaacgaga tcgaacggcc ctcggccttg
     2821 ggaggctcgt ggtgcccgcg ccgctcccgg cgcagccccc ggacctcgaa gatgtcggcg
     2881 aagtcggcgc cgaacgtgta cgtgacgtcc acctccaccg tccgggcgtt gtagttcttg
     2941 atcctcgtct tctcgaagag gcggtcgccg acggcgcgga tccgccggac gttgaggctc
     3001 tgctgggcgg ccgccacccg cccgtcggcg tcgtagagcg cggggttcgt catgtcgatg
     3061 tgggccatgt acgcgcgctc ggccgacgag gagagcagga tcgggtcgcg ccccgacagc
     3121 ttgaagcgga agtaggagag gaacctcgtg tcgaggtggt agaagcccag gccgagctcc
     3181 tgccggtcgt cgaggtgccc ctcggagtcg gcgtagagga aggtcggccc ctccttcgtg
     3241 gccagcacct tgcccggcag gtcggtgacg atctcgggcg cgccctcgtt caggatgcgc
     3301 tcgagggcgt cctggaagcg gggggagtcg ggtaactccg ggccggaggt catctccgcg
     3361 ggagtgtaaa gaggacctgc gcccggaaca agcgctcagg cctccgcgat cgaggcgccc
     3421 cccgaaggca ccccgggacg ctccacgcgc cagcccgggc cgagctcggg gatcgggccg
     3481 cccagaccct cgaaggccag gatcgtgggg ccggccccgg ccacacacgc ggggatctcg
     3541 gcgtcccgca ggtcgcggaa caacgcccga gcgccgggcg cgagctccag ccggcgagcc
     3601 tggtggagcc ggtcctcgag ggcctcgtgg agcagcgcag ggtcgtgggt cagcgcgcgg
     3661 acggccaggg ccgatcggcc gagattgaag gccgcgtcgc gcagcggcac atcgaggggc
     3721 aggtcgcggc gggcgtcctg ggtcgggacc cgctccccct ccgggatcag gacgaccggc
     3781 gcgaggtcgg gatggggctc gaggcgctcg gcgcgccagc cgtcgcggga gaggtacgcg
     3841 atcacgccgc ctccgcggag acaggccgcg acgttgtcgg ggtggccctc gacgtccacc
     3901 gccagcccca ggagccggtc gggatcgagg cccgcgccca ggagccggtc ggccagcagg
     3961 accccggcca ccaccgcgga ggccgaggaa cccaggcccc gctccagcgg gatctcgttg
     4021 cggcaccgga gccggaggga gggaagcgag ccgcccgcct cccgggccac gaaggccatg
     4081 gaccggaaga tgaggttgga ggcgtcctcg gggagctcgc cggcgccctc gcccgagacc
     4141 tcgacggcgg gctccgcgga cgtctcgcag acgacctcgt tgtggaggcc gagggcgagg
     4201 gcgagggcgt cgaagcccgg gccgaggttc gctgcggtgg ccgggacgcg gaccgtgagg
     4261 cgcatccccc tagagcccca gggcggcggc gaccgcgccg gggtcggcct ccacgcgagc
     4321 cgggcggggc gcgatcgccg cc
//
LOCUS       DASUTW010000345         2755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_120176, whole genome shotgun sequence.
ACCESSION   DASUTW010000345 DASUTW010000000
VERSION     DASUTW010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120176"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..654)
                     /gene="hisB"
                     /locus_tag="VF058_11860"
     CDS             complement(73..654)
                     /gene="hisB"
                     /locus_tag="VF058_11860"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX6209037.1"
                     /translation="MTRAAKVERKTKETSIRITLAVDGSGDSSAETGLPFFDHMLEQL
                     GKHARWDLEVRAEGDLEVDAHHTVEDVGLGLGQALREALGDKVGIRRFGSAAVPLDEA
                     LVEVALDLSARPYVVHEVPVPAEMIGTYDPGLTQDFVQAFATEAGMTIHCRLISGRSP
                     HHVAEAEFKAIAVALRSACEEIGGGTPSTKGSL"
     gene            complement(651..1718)
                     /gene="hisC"
                     /locus_tag="VF058_11865"
     CDS             complement(651..1718)
                     /gene="hisC"
                     /locus_tag="VF058_11865"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013019219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX6209038.1"
                     /translation="MTKPPLRPDLHSLSAYLAPQIDAPVRLNTNECPYPPAEAFAREL
                     GEVVARTPFHRYPDRDATALRKALAELTGHPVEGVWAANGSNEVIQHLCLAYGGPGRA
                     AMVFEPTYSLHRLIPRMVGMEIVEARLGDGFLLDERAVESVADRRPSIVFVCSPNNPT
                     GNAQPADLVAPLCEATDGLVIVDEAYGEFGGATAQGLVTERDNLVVARTFSKAFSMAA
                     ARLGYALAPAAIVEDLAKVRLPYHLSSLTQAAGEVALRHLEEARALLDRIREQRDRIL
                     AELGSIEGVEVFSSDANFVLFRTEVEAEALWRALLDRGVLVRDVSAYPGLERCLRVTA
                     GTPEETGAFLEALPEALEEVA"
     gene            complement(1715..>2755)
                     /gene="hisD"
                     /locus_tag="VF058_11870"
     CDS             complement(1715..>2755)
                     /gene="hisD"
                     /locus_tag="VF058_11870"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX6209039.1"
                     /translation="PELAAALDAMSERLLDLHRRQVPVPWEDERDGVRFGETVRPVWA
                     AGCYVPGGRAAYPSSVLMTTAPARAAGVERVVLCPPPAQDGSVPDAVLYAARAGAAHE
                     VFRIGGAQAIGAMAFGTETVPAVDTIAGPGNVWVTAAKREVMGTVGVDALAGPTELAI
                     VADGTADPSVLAVDLVAQCEHDPMARTTLIALDPGLPGAIEGPLAAELEASPRGEVVR
                     AALEHAVAVLAPDEDAAASVVDALAPEHLQVVTGDPRGFLSRVRSYGAAFLGPHTAVT
                     LGDYGVGSNHVLPTMATARFASGLRAAHFVTVSSWTQASPEGAARFGPEVEAVARAEG
                     LDAHARASEVRR"
BASE COUNT          367 a         1068 c          969 g          351 t
ORIGIN      
        1 cgacgcgctc caccgcgcgc tggacggacc tcaggttccc gaagccgtag tcgaggatcg
       61 ccaccgaggt ggtcacaggg accccttcgt ggacggcgtc cccccgccga tctcctcgca
      121 cgcgctgcgc agcgccaccg cgatcgcctt gaactcggcc tcggcgacgt ggtgcgggct
      181 gcggcccgag atcagccggc agtggatcgt catcccggcc tcggtggcga aggcctgcac
      241 gaagtcctgc gtcagcccgg ggtcgtaggt cccgatcatc tcggcgggca ccggcacctc
      301 gtgcacgaca tagggccgcg cggagaggtc cagcgccacc tcgaccagcg cttcgtccag
      361 cgggaccgcc gcgctgccga accggcggat ccccaccttg tcgcccagcg cctcccgcag
      421 ggcctgcccc agcccgagtc ccacgtcctc gacggtgtga tgggcgtcca cctcgagatc
      481 gccctcggcc ctgacctcca gatcccagcg ggcgtgcttc cccagctgct cgagcatgtg
      541 gtcgaagaag ggcaggccgg tctccgcgct gctgtcgccg gagccgtcca cggccagagt
      601 gatccggatg ctcgtctcct tcgtcttccg ctcgaccttc gccgctcgcg tcacgcgacc
      661 tcctccaggg cctcgggcag ggcctccagg aaggcccccg tctcctcggg ggtgcccgcc
      721 gtcacccgca ggcaccgctc cagcccaggg tacgcggaga cgtcgcggac gagcacgccc
      781 cggtcgagca gggcccgcca aagggcctcc gcctccacct cggttctgaa cagcacgaag
      841 ttggcgtcgg acgagaagac ctccacgccc tcgatgctcc cgagctcggc caggatccgg
      901 tcgcgctgct cgcggatcct gtcgaggagg gcgcgggcct cctccaggtg gcgcagagcc
      961 acctcgcccg ccgcctgggt cagcgaggag aggtggtaag ggagccgcac cttcgccagg
     1021 tcctccacga tcgccgccgg cgccagcgcg taccccaacc gggcggcggc catggagaac
     1081 gccttggaga aggtccgcgc gaccacgagg ttgtcacgct ccgtgaccag cccctgcgcc
     1141 gtggcgccgc cgaactcccc gtaggcctcg tccacgatca cgaggccgtc cgtcgcctcg
     1201 cacagcggcg cgaccaggtc ggccggctgg gcgttccccg tggggttgtt cggcgagcac
     1261 acgaacacga tcgagggccg gcggtcggcc acggactcga ccgcccgctc gtcgagcagg
     1321 aagccgtcgc ccagcctcgc ttcgacgatc tccatcccga ccatgcgggg gatcagccgg
     1381 tggagcgagt acgtcggctc gaacaccatg gcggcgcgcc cggggccgcc gtaggccagg
     1441 cacaggtgct ggatcacctc gttggagccg ttcgcggccc aaaccccctc gaccgggtgg
     1501 ccggtgagct cggccagggc cttccgcagc gccgtcgcgt cgcggtcggg gtagcggtgg
     1561 aatggggtgc gcgccacgac ctctcccagc tctcgggcga acgcctcggc cggaggatag
     1621 gggcactcgt tggtgttgag acgcaccggc gcgtcgatct ggggcgccag gtaggcggag
     1681 agcgagtgca ggtcggggcg gaggggcggc ttcgtcatcg gcgcacctcg ctggccctgg
     1741 cgtgggcgtc caggccctcc gccctggcca cggcctcgac ctcgggcccg aaccgcgcgg
     1801 ccccctccgg cgaggcctgc gtccacgagg acaccgtcac gaagtgggcc gcccgcaggc
     1861 cggaggcgaa ccgggccgtg gccatcgtgg gcaggacgtg gttcgagccc acgccgtagt
     1921 cgcccagcgt gacggctgtg tggggcccga ggaacgcggc gccgtaggag cgcactcgtg
     1981 acaggaaccc tcgcgggtcg cccgtcacca cctggaggtg ctcgggggcg agggcgtcca
     2041 ccaccgaggc ggccgcgtcc tcgtcgggag ccagcaccgc caccgcgtgc tccagcgccg
     2101 cgcggacgac ctcgccccgc gggctggcct cgagctcggc cgccaagggg ccctcgatcg
     2161 cgcccgggag acccggatcc agcgcgatca gcgtggtgcg ggccatcggg tcgtgctcgc
     2221 actgcgcgac gaggtccacg gcgagcacgg acgggtcggc cgtcccgtcc gccacgatcg
     2281 ccagctcggt ggggccggcc agggcgtcca cacccaccgt ccccatcacc tcccgcttcg
     2341 ccgccgtgac ccacacgttc ccgggaccgg cgatcgtgtc cacggcgggc acggtctcgg
     2401 tcccgaaggc catcgccccg atggcctggg cgccaccgat ccggaagacc tcgtgggcgg
     2461 cgcccgcgcg cgccgcgtac agcaccgcgt cggggacgct cccgtcctgg gccggcgggg
     2521 ggcagaggac cacccgctcc acccccgcgg cgcgcgcggg cgccgtcgtc atcagcacgc
     2581 tcgaggggta cgccgcccgc cctccgggca cgtagcagcc cgcggcccac accgggcgca
     2641 cggtctcgcc gaaccggacg ccgtcgcgct cgtcctccca aggcaccggc acctgccggc
     2701 ggtggaggtc cagcagtcgc tccgacatcg cgtcgagcgc cgcggccagc tcggg
//
LOCUS       DASUTW010000346         3438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1803022, whole genome shotgun sequence.
ACCESSION   DASUTW010000346 DASUTW010000000
VERSION     DASUTW010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803022"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="VF058_11875"
     CDS             complement(<1..990)
                     /locus_tag="VF058_11875"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HEX6209040.1"
                     /translation="MSEVPVLGSVPGQDEAIAFLERAAGRPGHAYLFAGPEGSGKKLA
                     AAAFTAALLCERGGCGKCRDCRLALEDRHPNVAVLEPEGRDIRVGEGTDDFGTARWLV
                     ARAHRTAPEPGRKVFRIEQADRMTEEAADVLLKVVEEPPPETVFLLMSARPHELTETI
                     RSRCQTVAFHPLAEAFVVETLTAEGVSEGRGMLASRLAGGNVGRARRIARDPEGLSFR
                     DEALAALREVGGDPRGALAGAQRLVDAAADYKKGLSRVLDEELEPLLDEETGRVAEQF
                     RGLARRIDERHKRRERRAEREFLDWSLLALEAWYRDALLISTGGDPSWVINMDL"
     gene            complement(987..2987)
                     /gene="tmk"
                     /locus_tag="VF058_11880"
     CDS             complement(987..2987)
                     /gene="tmk"
                     /locus_tag="VF058_11880"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX6209041.1"
                     /translation="MVDYADEIAALRGTKPATFGSLFRNRSFFRLWRAMLVSSLGDWT
                     GFVAITALVTRIGGPRAGYAVTGVMLARLLPSLIFGPVAGVFVDRFDRRKLMVIADIG
                     RGAGYVSMAFVSELWLIYALSFSVECLSLLWTPARDASIPNIVSRRQLSNANSMGLFT
                     SYGTLPLGGILFTALTALATGIGGVIEYFGDRPESLALLLNSVTFAFSAWMIWGLQLP
                     ERSRRAQGRLRPAQAWDDLREGIRFLRDHAFQRTMTIGVVLAFTGVGSVMALGPIFAQ
                     NTLGAGQAGWGILVTSVGLGLAAGMISVGLVERVVDKETLFPLAMIGAAGLLVVLSLM
                     PNIALAAMLTAVMGSVAGLAWVAGYTLLHENVGDEFRGRTFATLTILSRLGLLASLAG
                     FPALAQTIGDYSIGIGEAEIDLAGTRLALWVGAAVVLAGGVSARRGLRRGRRSRAQPL
                     QLRPHLRRGERPGTFIVFEGVEGSGKGTQVELAKDFLEGEGYEVLVTREPGGTPLGEG
                     LREVLLGDQPLDARAEALLFAAGRAQHAVAVIRPALEKGRVVLCDRYVDSSLAYQGVG
                     RDLGEQDVLALSAWATQGLFPDLVILLNIDPEEGLERSGGDDRFESEGDEFHEKVAQA
                     YLRIADEHPERVVVVDALGTPEEVQGRVRDAIRRFLKGQEEA"
     gene            complement(2989..>3438)
                     /locus_tag="VF058_11885"
     CDS             complement(2989..>3438)
                     /locus_tag="VF058_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HEX6209042.1"
                     /translation="VDGQERPEPELLEETCPECGKQLVKRWGRYGPFIGCSGYPDCRY
                     IKREEKKTGVTCPKCGEGELIEKRARRRRGNVFYGCNRYPDCDFTVGQRPLTEPCPEC
                     GALMTAQKDGSGKCQNGHVIEGAAERSAPGSEEKEPEASGSGSAEAG"
BASE COUNT          511 a         1369 c         1103 g          455 t
ORIGIN      
        1 gagatccatg ttgatcaccc acgaggggtc tccgccggtc gagatcagca gggcgtcccg
       61 gtaccaggcc tcgagggcga gcagggacca gtcgaggaac tcccgctccg ccctccgctc
      121 cctccgcttg tggcgctcgt cgatccgcct ggccagcccc cggaactgct cggccacccg
      181 gcccgtctcc tcgtcgagca gcggctccag ctcctcgtcg agcacgcggc tcagcccctt
      241 cttgtagtcg gccgcggcgt ccacgagccg ctgggccccg gccagcgccc cccgcgggtc
      301 ccccccgacc tcgcgcagcg cggcaagggc ctcatcgcgg aacgagaggc cctcgggatc
      361 gcgggcgatc cggcgggccc ggcccacgtt ccccccggcc agacgcgagg cgagcatccc
      421 ccgcccctcg ctcacaccct ccgctgtgag cgtctccacc acgaacgcct ccgccagcgg
      481 atggaacgcc accgtctggc accgcgagcg gatcgtctcg gtcagctcgt ggggccgcgc
      541 cgacataagg aggaacaccg tctctggcgg cggctcctcg accaccttca gcaggacgtc
      601 ggccgcctcc tcggtcatcc gatcggcctg ctcgatccgg aacaccttcc tccccggctc
      661 gggcgccgtt cggtgggcgc gggccacgag ccaccgggcc gtcccgaagt cgtccgtccc
      721 ctcacccacg cggatgtccc ggccctccgg ctcgagcacc gccacgttgg ggtggcggtc
      781 ctccagcgcc aggcggcagt cccggcactt gccgcagcct ccccgctcgc acaggagcgc
      841 ggcggtgaag gccgcggcgg cgagcttctt ccccgacccc tcgggccccg cgaacaggta
      901 ggcgtggccg gggcgccccg ccgcccgctc caggaacgcg atcgcctcgt cctgccccgg
      961 cacggagccc aggaccggga cctcgctcac gcctcctcct gccccttcag gaagcgccgg
     1021 atcgcgtcgc gcacccgccc ctggacctcc tccggcgtcc ccagcgcgtc caccacgacg
     1081 actcgctcgg ggtgctcgtc ggcgatccgg aggtaggcct gggccacctt ctcgtggaac
     1141 tcgtctccct cggactcgaa gcgatcgtcg ccccccgacc gctccaggcc ctcctcggga
     1201 tcaatgttga gcaggatcac gaggtcgggg aagagcccct gcgtggccca ggccgagagc
     1261 gcgaggacgt cctgctcccc cagatcgcgc cccaccccct gataggcgag cgaggaatcc
     1321 acgtaccggt cgcacaggac gaccctgccc ttctccagcg ccgggcggat cacggccacc
     1381 gcgtgctggg cccggccggc ggcgaagagc agcgcctcgg ccctcgcgtc gagcggctgg
     1441 tcgcccagca gcacctcccg gagcccctct cccagcggcg tcccgcccgg ctcccgggtg
     1501 accaggacct cgtacccctc gccctcgagg aagtccttcg ccagctcgac ctgcgtgccc
     1561 ttgccggatc cctccacccc ctcgaagacg atgaaggtcc ccggccgctc gccgcgtcgg
     1621 agatgtgggc ggagctggag cggctgcgcc ctagagcgcc gtccccgtcg gagccccctc
     1681 cgtgcgctca cgccgcccgc caggacgacg gccgcaccga cccacagggc cagacgcgtg
     1741 ccggccagat cgatctcggc ctcgccgatc ccgatcgagt agtcgccgat cgtctgggcc
     1801 agcgcgggga agcccgccag cgaggcgagg agaccgagcc tcgacaggat cgtgagcgtg
     1861 gcgaaggtcc ggcctcggaa ctcatcgccc acattctcgt ggagaagggt gtaacccgcg
     1921 acccacgcca gccctgccac cgaccccatc accgccgtca acatcgcggc cagcgcgatg
     1981 ttcggcatca gcgacagcac caccagcagc ccggccgctc cgatcatcgc cagggggaag
     2041 agggtctcct tgtcgaccac acgctcgacg agcccgaccg agatcatccc cgccgcgagg
     2101 ccgagcccca ccgacgtcac gaggatcccc caccccgcct ggcccgctcc cagcgtgttc
     2161 tgggcgaaga tcgggccgag cgccatcacg gaccccacgc cggtgaaggc cagcaccacc
     2221 ccgatcgtca tcgtccgctg gaaggcgtgg tcgcggagga accggatccc ctcccgcagg
     2281 tcgtcccagg cctgcgccgg gcgcagcctg ccctgggccc gacggctccg ctccgggagc
     2341 tggagacccc agatcatcca cgcggagaag gcgaacgtga cgctgttgag cagcagcgcc
     2401 aacgactcgg gccggtcccc gaagtactcg atcacgcctc cgatgccggt cgccagcgcg
     2461 gtgagcgcgg tgaagaggat ccccccgagg ggaagcgtcc cgtacgaggt gaacaacccc
     2521 atcgaattgg cgttggagag ctgccgccgg gacacgatgt tcgggatgct cgcgtcacgg
     2581 gccggggtcc acagcagcga gaggcactcc accgagaacg agagcgcgta gatcagccac
     2641 agctcgctga cgaaggccat cgacacgtac ccggcccccc ggccgatgtc ggcgatcacc
     2701 atcagcttcc tgcggtcgaa gcgatcgacg aagacccccg ccacgggccc gaagatcagc
     2761 gaagggagca gacgtgccag catgacgccg gtgacggcgt agccggcgcg ggggcccccg
     2821 atccgggtga ccagggccgt gatcgcgacg aagccggtcc agtcccccag cgaggagacg
     2881 agcatcgccc gccacaggcg gaagaacgat cggttcctga agaggctgcc gaaggtcgcg
     2941 ggcttcgtgc cccgaagcgc cgcgatctcg tcggcgtagt cgaccaacct agcccgcttc
     3001 cgccgacccg gagccggagg cctccggctc cttctcctcg ctcccggggg ccgagcgctc
     3061 cgccgccccc tcgatcacgt gcccgttctg gcacttcccg ctgccgtcct tctgcgcggt
     3121 catcagcgcg ccgcactcag ggcagggctc ggtcagcggc cgctggccga cggtgaagtc
     3181 gcagtctggg tagcggttgc agccgtagaa gacgttgccg cgccggcggc gggccctctt
     3241 ctcgatcagc tcgccctccc cgcacttggg gcacgtcacg ccggtcttct tctcctcccg
     3301 cttgatgtag cggcagtccg ggtagccgga gcagccgatg aacggcccgt accgccccca
     3361 ccgcttcacg agctgcttgc cgcactcggg gcaggtctcc tcaagcagct cgggctcggg
     3421 tcgctcctgg ccgtccac
//
LOCUS       DASUTW010000347         3641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1675802, whole genome shotgun sequence.
ACCESSION   DASUTW010000347 DASUTW010000000
VERSION     DASUTW010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675802"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1348
                     /locus_tag="VF058_11890"
     CDS             <1..1348
                     /locus_tag="VF058_11890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6209043.1"
                     /translation="IEVSSHEYVVTGAPDTKFGTPVAGGMAGSALKRALAADHLDVRG
                     IHAHVGSQLLDRAPYLGALEVALDFLREARDDAGFEAALLDLGGGMGARYTDEEVPAP
                     AEIAEPVREALAWGCEERGLPVPTLIAEPGRAISSSAACTLYRVGSIKEVPGIRTYVA
                     VDGGMSDNIRPALYDARYTVGVASRASTAEPAEVTIVGRHCESGDVLARDATLPEDLR
                     RGDLVAFASTGAYEYAMASTYNKVGRPAVVLVGGDGEARPILRREELGDLARLDVGPP
                     PTDRAPAPPGIEVRPARARDARGVMDLVTEVAAEGRFIRTDRVAPSAWPYRARLRRSW
                     TSDGAEIVALEDGKVVAHLGISRESVPALRHVAAIGMSVRRDHRGRGVGSALMSEAIR
                     WAREMGVEKLSLSVFPHNASAIALYRKFGFVEEGVFRGHTRKSYGYEDEVVMARWV"
     gene            1339..2619
                     /locus_tag="VF058_11895"
     CDS             1339..2619
                     /locus_tag="VF058_11895"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX6209044.1"
                     /translation="MGVRTLTVGFLGCGTVGAATLRLLDEHAEDIAERGGCRIEVARV
                     GVRDPRRERDVPVPADRFTDDLRSVVDDPDVEAVIEVMGGMDPALDLVLRALEKGKHV
                     VTANKELLASSGRDLFAASESAGVDLRFEAAVGGGIPLIRPLKESLAGERVARVTGIV
                     NGTTNFVLTRMHEDGMSFDEALGLAVEQGFAEPDPTADVEGLDAAAKCAILASIAFNS
                     RVVADDVAREGVGGVSREDVAFAEQLGYVVKPLAVAELDGDDVAVRVHPAMLPTDHPL
                     AAVRESFNAVFVEGPNVGELMFYGRGAGGEPTAVAVVGDLVDVARSGGRQVGCTCYRE
                     RRILSIEESEGRFYLLLAVVDRPGVLARIAGVFGEEGVSIRSVWQEGTGEEALIVMIT
                     HRANEGALRRTVDGLRGLDVVHEVRSVMRVEGEG"
     gene            2619..>3641
                     /gene="thrC"
                     /locus_tag="VF058_11900"
     CDS             2619..>3641
                     /gene="thrC"
                     /locus_tag="VF058_11900"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX6209045.1"
                     /translation="MAAPWRGIVFEYGDRLPVTSATPIVTLGEGGTPLLHSPSLSEET
                     GCEVYLKYEGANPTGSFKDRGMTVAISRALEQGAEAVVCASTGNTSASAAAYAARAGM
                     TCGVLVPRGRVASGKLAQALVHGARILEIDGSFDEALTVVRELADGSAVTLVNSVNPD
                     RIEGQKTLAFEVCDALGRAPDYHLLPVGNAGNITSHWRGYGEYAADGLIAEPPAMLGF
                     QAAGAAPIVRGEPVPKPTTIATAIRIGNPASWEGAEKAAAESGGAIEAVTDRQILEAY
                     RRLAREGIFCEMASAASVAGLLQVSALDRLERGRTAVCVLTGHGLKDPEWAAATAPRP
                     ARVEADP"
BASE COUNT          471 a         1210 c         1485 g          475 t
ORIGIN      
        1 gatcgaggtc tcgagccacg agtacgtggt gacgggggct cccgacacga agttcgggac
       61 gccggtggcc ggggggatgg ccgggagcgc gctgaagcgt gcgctggccg cggaccacct
      121 cgacgtgcgg gggatccacg cccacgtggg gtcgcaactc ctggaccgcg ctccctacct
      181 cggggcgctg gaggtggccc tggacttcct gcgggaggcc agggacgacg cggggttcga
      241 ggccgcgctg ctcgatctcg gcggggggat gggggcccga tacacggacg aggaggtgcc
      301 ggcacccgcc gagatcgcgg agccggtgcg cgaggcgctg gcctgggggt gcgaggagcg
      361 aggcctgccg gtccccacgc tgatcgcgga gcctggccgc gcgatctcgt cctcggccgc
      421 ctgcaccctg taccgggtgg gatccatcaa ggaggtgccc ggcatccgga cgtacgtggc
      481 ggtggacggc ggcatgtccg acaacatccg gcccgcgctg tacgacgcaa ggtacacggt
      541 gggggtggcg tcgcgtgcga gcacggccga gccggccgag gtcacgatcg tcggccgcca
      601 ctgcgagtcg ggcgacgtgc tcgcacgcga cgccaccctc cccgaggacc tgaggcgcgg
      661 cgacctcgtg gccttcgcct ccacgggggc ctacgagtac gcgatggcca gcacctacaa
      721 caaggtgggg cgccccgccg tggtgctggt gggcggcgac ggggaggccc gcccgatcct
      781 ccggagggag gagctcgggg acctggcccg gctcgacgtg gggccgcccc ccaccgaccg
      841 cgccccggcc cccccgggga tcgaggtccg cccggcccgg gcccgggacg ctcgcggcgt
      901 gatggacctc gtgaccgagg tcgcggccga ggggcgcttc atccggacgg accgggtggc
      961 cccctccgcc tggccctacc gcgcccggct ccgccggtcg tggacgtccg acggcgccga
     1021 gatcgtggcg ctggaggacg gcaaggtcgt ggcgcacctc gggatctccc gggagtccgt
     1081 gcccgccctc cgccatgtgg cggcgatcgg gatgtcggtc cgccgcgacc accggggccg
     1141 cggggttggc tccgcgctga tgtccgaggc gatccgctgg gcccgcgaga tgggcgtgga
     1201 gaagctgagc ctctccgtct tcccccacaa cgcctccgcg atcgcgctct accggaagtt
     1261 cgggttcgtc gaggagggcg tcttccgggg ccacacgagg aagtcgtacg gctacgagga
     1321 cgaggtggtg atggcccgat gggtgtgagg accctgacgg tcgggttcct cggctgcggc
     1381 acggtgggcg ccgcgaccct gcgcctcctg gacgagcacg ccgaggacat cgccgagcgg
     1441 ggcgggtgcc gcatcgaggt ggcccgcgtg ggcgtgcggg acccccggcg cgagcgcgac
     1501 gtccccgtgc ccgccgatcg cttcacggac gacctgcgct ccgtggtgga cgaccccgac
     1561 gtcgaggccg tgatcgaggt gatgggcggg atggatcccg ccctcgacct ggtcctgcgc
     1621 gcgctcgaga agggcaagca cgtcgtgacg gcgaacaagg agctgctggc ctcgagcggc
     1681 cgcgatctct tcgccgcgtc cgagagcgcg ggcgtggacc tgcgcttcga ggccgccgtg
     1741 ggcgggggca tcccgctgat ccgtccgctg aaggagtcgc tggcgggcga gcgcgtggcc
     1801 cgcgtcaccg ggatcgtcaa cgggacgacg aacttcgtgc tgacccggat gcacgaggac
     1861 ggcatgtcgt tcgacgaggc gctcggcctc gcggtcgagc agggcttcgc ggaacccgac
     1921 cccaccgccg acgtcgaggg gctcgacgcg gccgcgaagt gcgcgatcct cgcctcgatc
     1981 gccttcaact cgcgcgtggt cgcggacgac gtggcccgcg agggggtggg cggggtgagc
     2041 cgggaggacg tggctttcgc cgagcagctc ggctacgtgg tgaagccgtt ggctgtggcc
     2101 gagctcgacg gagacgacgt ggccgtccgc gtgcaccccg ccatgctgcc gaccgaccac
     2161 ccgctggccg ccgtccgcga gagcttcaac gcggtcttcg tggaggggcc gaacgtcggg
     2221 gagctgatgt tctacgggcg gggcgcgggg ggcgagccca ccgcggtggc cgtcgtgggg
     2281 gacctcgtgg acgtggcccg gagcggcggt cggcaggtgg gatgcacgtg ctaccgggag
     2341 cggcgcatcc tgtcgatcga ggagagcgag gggcggttct acctcctgct cgcggtggtg
     2401 gaccggccgg gggtgttggc ccgcatcgcc ggggtcttcg gcgaggaggg ggtctcgatc
     2461 cgcagcgtgt ggcaggaggg gacgggcgag gaggcgctga tcgtgatgat cacccatcgc
     2521 gcgaacgagg gcgccctgcg acggacggtg gacgggctgc gcgggctcga cgtcgtgcac
     2581 gaggtgcgga gcgtcatgcg cgtcgagggc gagggctgat ggcggcgccc tggcggggca
     2641 tcgtcttcga gtacggcgac cggctcccgg tgacgagcgc cacgccgatc gtgacgctcg
     2701 gggagggagg cacgccgctg ctccactcgc cctcgctctc tgaggagacc ggctgcgagg
     2761 tctacctgaa gtacgagggc gccaacccca cgggatcctt caaggaccgg gggatgacgg
     2821 tggcgatctc gcgggcgctg gagcaggggg ccgaggcggt ggtctgcgcc tccaccggca
     2881 acacgagcgc ctcggcggcg gcctacgcgg cgcgggccgg gatgacctgc ggggtgctgg
     2941 tgccgcgggg gcgcgtggct tccgggaagc tggcccaggc cttggtgcac ggcgcgcgga
     3001 tcctggagat cgacggaagc ttcgacgagg cgctgaccgt cgtgcgggag ctggccgacg
     3061 gcagcgcggt cacgctcgtc aactccgtga accccgaccg gatcgagggg cagaagaccc
     3121 tcgccttcga ggtgtgcgac gcgctgggcc gggcgcccga ctaccacctg ctcccggtgg
     3181 ggaacgcggg caacatcacg agccactggc ggggctacgg cgagtacgcg gccgacgggc
     3241 tgatcgccga gccgccggcg atgctggggt tccaggcggc gggggccgcg cccatcgtgc
     3301 gcggcgagcc ggtgccgaag ccgacgacga tcgccaccgc gatccggatc gggaacccgg
     3361 cctcgtggga gggggccgag aaggccgccg ccgagtcggg cggggcgatc gaggcggtga
     3421 ccgaccggca gatcctggag gcgtaccggc gcctggcccg cgaggggatc ttctgcgaga
     3481 tggcctcggc ggcgtcggtg gcggggctgc tgcaggtctc ggccctggat cggctggagc
     3541 gagggaggac ggccgtgtgc gtgctgaccg ggcacgggct gaaggacccg gagtgggcgg
     3601 cggcgaccgc gccccgcccg gctcgcgtgg aggccgaccc g
//
LOCUS       DASUTW010000348         4840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1547118, whole genome shotgun sequence.
ACCESSION   DASUTW010000348 DASUTW010000000
VERSION     DASUTW010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547118"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VF058_11905"
     CDS             complement(<1..791)
                     /locus_tag="VF058_11905"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6209046.1"
                     /translation="MGLLRIRDLRIVLLAYGLSAFGDYLALIALTLRIKDLTESGLAV
                     SALLVAGILPLVVLGPLAGHVVDRLEPTRVLTFGALIQAGLAFALVFAEEPWLIITLF
                     FLLSTGFALSQPGIFAMTPWIVGEDNTTEANAYLEVSRWAGAAIGPIVAGVISHTWGS
                     DAALLLNALTFLAVGLAMPLLRVRRAADATPPATPERARDGVVFIGRDPILRVVVVAI
                     TLMVVFSAIDNVAEVFFAKDVLEAGDLGYSGLVTAWTLGMVIGATG"
     gene            923..2809
                     /gene="ftsH"
                     /locus_tag="VF058_11910"
     CDS             923..2809
                     /gene="ftsH"
                     /locus_tag="VF058_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX6209047.1"
                     /translation="MKPRRPFRGPVLYLILVLGALWLFLWMGDRSPEAAELSVTELES
                     LAGEGAVVSAEFMERDQRVEGELASGERYTAPYLGEYGDDLLETLQRSGVEVSANPQP
                     DPVFVRILNFLPLLLIFGLIVWIFVQQSGGGGGSRVMSFGRAKARQVTKDMPKTTFED
                     VAGVEEAIEELQEVKEFLENPKKFEAMGARIPKGVLLFGPPGTGKTLLARAVAGEAGV
                     PFFSISGSDFVEMFVGVGAARVRDLFQQAKEAAPAIVFIDEIDAVGRHRGAGLGGGHD
                     EREQTLNQLLVEMDGFDARTGVILMAGTNRPDILDPALLRPGRFDRQIVVDYPDLNGR
                     KGILAVHARGKPLAKGVSLDDIAKQTPGFTGADLANLMNEAALLAARRGKERISKAEL
                     EEAIDRVLMGPEKKTRLISDKEKRVIAYHEAGHALVGFAHPDVDPVHKVTVIPRGRAG
                     GYTLVLPTEDRMLNTRSELLGQLAYGLGGRVAEQLVFGDITTGANDDLQKASKLARRM
                     VTEFGMSDLLGPITLGQRQHEVFLGKDIAHTPDYSDQVAFEIDTEVRRLIDEAHDEAL
                     ATLKRNRKKLDGLAEALMERETLDKDEVAEILKGVRGGGRKNGERGAGIAAFRRSPGG
                     GRRS"
     gene            2809..3384
                     /gene="folE"
                     /locus_tag="VF058_11915"
     CDS             2809..3384
                     /gene="folE"
                     /locus_tag="VF058_11915"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016421488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX6209048.1"
                     /translation="MPEFDERKAEEAVERLLEAIGEDPGRDGLKETPRRVAAMYREIF
                     AGMRQDPEVVLDVTFEAGHSEMVMVREMPLYSMCEHHLVPWVGKAHVAYIPGKDGRIT
                     GLSKLARLVEVFAKRPQVQERLTTQIADTIEKVLKPRGVFVVIEAEHLCMTMRGVRAP
                     GSVTVTSAVRGVFKSDARTRAEAMSLIRRLD"
     gene            3384..3740
                     /locus_tag="VF058_11920"
     CDS             3384..3740
                     /locus_tag="VF058_11920"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX6209049.1"
                     /translation="MAGPASVEEYLASLPGVARAYLEELRATILSVVPGATETISYQM
                     PAVRSGRRIVVWYAAFKDHYSVFPATEGVREALGPEVEPYLSGKGTIRLPADEPVPVS
                     LVERIVEARLRENASA"
     gene            complement(3737..4108)
                     /locus_tag="VF058_11925"
     CDS             complement(3737..4108)
                     /locus_tag="VF058_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6209050.1"
                     /translation="MTLTPEQWRELEAIGLPPLPEPPAPPGPRALDDVLATRTHVKVL
                     RVLTLDRSYNFTARGLARTAETSRTRALDVVRHLGSIGMLIGHHTHTHSIYRIRDTHP
                     LTPALTVLFEVEAELAHRSEA"
     gene            complement(4264..>4840)
                     /gene="pdxH"
                     /locus_tag="VF058_11930"
     CDS             complement(4264..>4840)
                     /gene="pdxH"
                     /locus_tag="VF058_11930"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX6209051.1"
                     /translation="APSDPMERFRRWWEEARTLLGEEADVMVVATAGADGRPSARNVL
                     LRGFGPEGFVFYTNLRSRKARDLAATSYAALALYWRDIGRQVRVEGPVERTSRDEDEA
                     YWRTRPVGAQVAAWVSAQSDPIVSRDELVVAHDEALRRFGEDVPLPEHWGGYRVVPEA
                     IEFWQGREHRLHDRIRYERRGDAWTAHRLAP"
BASE COUNT          750 a         1599 c         1825 g          666 t
ORIGIN      
        1 ccggtggccc cgatcaccat ccccagggtc cacgccgtca ccaggccgct gtacccgagg
       61 tcgcccgcct ccagcacgtc cttggcgaag aagacctcgg ccacgttgtc gatcgccgag
      121 aagacgacca tcagcgtgat cgccaccacc accacgcgga ggatcgggtc gcggccgatg
      181 aagacgacgc cgtcgcgggc ccgctcgggc gtggccggcg gcgtcgcgtc ggcggccctc
      241 cggacccgga ggagaggcat ggccaggccc accgccagga acgtcagcgc gttcaggagg
      301 agcgcggcgt ccgagcccca ggtatgcgag atgaccccgg ccacgatcgg gccgatcgcg
      361 gctccggccc acctcgagac ctcgaggtac gcgttggcct cggtggtgtt gtcctcgccc
      421 acgatccacg gcgtcatggc gaagatcccg ggctggctga gcgcgaagcc cgtgctcagc
      481 aggaagaaga gggtgatgat cagccacggc tcctcggcga agaccagggc gaacgccagc
      541 cccgcctgga tcagcgcgcc gaacgtgagg acgcgggtcg gctcgagccg gtccacgacg
      601 tgcccggcca ggggacccag cacgacgagc gggaggatcc ccgccaccag gagcgccgac
      661 accgccagcc cggactcggt gaggtccttg atgcgcagcg taagggcgat gagcgccagg
      721 tagtctccga atgccgagag tccgtaggcg agcaggacga tccggaggtc ccggatccgc
      781 aggaggccca tgggcgattt gtaccgccgt tggggcggtc cgtccagccc cgggggccgg
      841 ctccgttcga gaggccctgt gctaccatcg ccgcaccgat cggccccgcc cctggatgga
      901 acgaaggaga cccccgaccg acatgaagcc ccgtcgcccc ttccgagggc cggtgctcta
      961 cctgatcttg gtcctcggcg cgctgtggct cttcctgtgg atgggggacc ggagccccga
     1021 ggccgccgag ctctccgtga ccgagctgga gagcctggcc ggcgaggggg ccgtcgtctc
     1081 cgccgagttc atggagcgcg accagcgggt cgagggtgag ttggcgagcg gcgagcggta
     1141 cacggctccc tacctcggcg agtacggcga cgacctgctg gagaccctcc agcggagcgg
     1201 ggtcgaggtg tccgcgaacc cccagcccga ccccgtgttc gtccgcatcc tgaacttcct
     1261 cccactcctc ctgatcttcg ggctgatcgt ctggatcttc gtccagcaga gcggcggcgg
     1321 gggcggctcc cgggtgatgt cgttcggcag ggccaaggcc cgccaggtca cgaaggacat
     1381 gcccaagacc acgttcgagg acgtggccgg agtcgaggag gcgatcgagg agctccagga
     1441 ggtgaaggag ttcctggaga accccaagaa gttcgaggcg atgggggcga ggatccccaa
     1501 gggggtcctc ctgttcggcc cgccggggac ggggaagacc ctgctggccc gggccgtggc
     1561 cggggaggcc ggcgtgccgt tcttctccat ctcgggatct gacttcgttg agatgttcgt
     1621 gggcgtcggg gccgcgcgcg tccgcgatct cttccagcag gcgaaggagg ccgccccggc
     1681 catcgtgttc atcgacgaga tcgacgccgt gggaaggcac cgcggcgcgg gcctcggggg
     1741 cggccacgac gagcgcgagc agacgctgaa ccagctgctg gtcgagatgg acgggttcga
     1801 cgcgcggact ggcgtgatcc tgatggcggg cacgaaccgt cccgacatcc tcgacccggc
     1861 gctcctgcgc ccgggtcgct tcgaccgcca gatcgtggtc gattacccgg acctcaacgg
     1921 gcgcaagggc atcctcgcgg tccacgcccg gggcaagccc ctggccaagg gcgtgagcct
     1981 ggacgacatc gcgaagcaga cgcccgggtt caccggggcc gacctggcca acctcatgaa
     2041 cgaggcggcg ctcctcgccg cgcgcagggg caaggagcgc atctccaagg ccgagctgga
     2101 ggaggcgatc gaccgggtgc tgatgggccc ggagaagaag actcgtctca tctcggacaa
     2161 ggagaagcgg gtgatcgcct accacgaggc gggccacgcg ctcgtggggt tcgcccaccc
     2221 ggacgtcgac ccggtgcaca aggtcaccgt gatcccgcgg gggcgggccg gcggctacac
     2281 gctcgtcctc cccaccgagg accggatgct caacacgcgg tcggagctgc tgggtcagct
     2341 cgcctacggg ctgggcggcc gcgtggccga gcagctggtc ttcggggaca tcacgacggg
     2401 ggccaacgac gacctccaga aggcctcgaa gctggcgcga cggatggtga cggagttcgg
     2461 gatgtcggac ctgctggggc cgatcacgct gggtcagcgc cagcacgagg tgttcctggg
     2521 caaggacatc gcccacaccc ccgactactc cgaccaggtc gcgttcgaga tcgacaccga
     2581 ggtgcggcgc ctgatcgacg aggcgcacga cgaggcgctg gccacgctga agcggaaccg
     2641 caagaagctc gacgggctcg cggaggccct gatggagcgg gagacgctgg acaaggacga
     2701 ggtggccgag atcctgaagg gcgtgcgggg cgggggccgg aagaacggcg agcggggcgc
     2761 cgggatcgcc gcgttccgcc gaagccccgg cggcggccgc cggagctgat gcccgagttc
     2821 gacgagcgga aggccgagga ggccgtcgag cgcctgctcg aggccatcgg cgaggacccc
     2881 ggccgagacg ggctgaagga gaccccccgc cgcgtggcgg cgatgtaccg cgagatcttc
     2941 gcggggatgc gccaggaccc cgaagtggtg cttgacgtca cgttcgaggc aggtcacagc
     3001 gagatggtca tggtgcgcga gatgccgctc tactcgatgt gcgagcacca cctcgtaccg
     3061 tgggtgggga aggcgcacgt ggcctacatc cccgggaagg acgggcggat cacggggctg
     3121 tcgaagctgg ccaggctggt ggaggtgttc gccaagcggc cgcaggtcca ggagcggctg
     3181 acgacgcaga tcgcggacac gatcgagaag gtgctgaagc ctcgcggggt gttcgtggtg
     3241 atcgaggccg agcacctctg catgacgatg cggggggtcc gggcgccggg gtcggtgacg
     3301 gtgacctccg cggtgcgcgg cgtcttcaag agcgatgcga ggacccgggc cgaggcgatg
     3361 agcctgatcc gccggctcga ctagtggcgg ggccggcgag cgtcgaggag tacctggcct
     3421 cccttccggg ggtggctcgc gcctatctcg aggagctcag ggcgacgatc ctctcagtgg
     3481 tcccgggggc gaccgagacg atcagctatc agatgcccgc ggtgaggtcg ggtcgccgga
     3541 tcgtcgtgtg gtacgccgcg ttcaaggatc actacagcgt gttccccgcc accgaggggg
     3601 tgagggaggc gctcggcccg gaggtcgagc cgtacctctc gggcaagggg acgatccggc
     3661 tgccggccga cgagcccgtg cccgtctctc tggtcgagcg gatcgtcgag gcccggctcc
     3721 gcgagaacgc gtcggcttaa gcctcactgc ggtgagcgag ctcggcctca acctcgaaga
     3781 ggacggtcag ggccggcgtg agggggtgcg tgtcgcggat ccggtagatc gagtgggtgt
     3841 gcgtgtggtg gccgatcagc atcccgatcg aaccgagatg tcgaacgacg tcaagcgcgc
     3901 gggtgcgtga ggtctcggcg gtgcgagcga gccctcgagc ggtgaagttg taggagcggt
     3961 cgagcgtgag aacgcggagg accttcacgt gggtcctggt cgcgaggacg tcgtcgaggg
     4021 ccctcggccc gggtggggcc ggcggctcgg gtagcggtgg caggcctatc gcctcgagct
     4081 cgcgccactg ctctggggtg agggtcatgg gcatcctgta tcacgagggt ctgacatggt
     4141 cggcggtcgc cagtctggtg cgctgaggtg ccccgcaagt gtgaccgcga tcccggccga
     4201 gtgaccgcga tcccggccac acctgcgggg aacccccccg ccggtccctc ggtgctctgg
     4261 ccgctacggc gccaggcggt gggcggtcca ggcgtcgccg cggcgctcgt agcggatgcg
     4321 gtcgtgcagg cggtgctcgc ggccctgcca gaactcgatc gcctcgggga ccacgcggta
     4381 gccgccccag tgttcgggga gcggcacgtc ctccccgaag cggcggagtg cctcgtcgtg
     4441 agccacgacc agctcgtcgc ggctgacgat cgggtcgctc tgggccgaga cccaggccgc
     4501 gacctgcgcc ccgaccggtc gcgtccgcca gtaggcctcg tcctcgtcgc gcgacgtccg
     4561 ctcgaccggg ccctcgaccc gcacctgcct tccgatgtca cgccagtaga gcgccagcgc
     4621 ggcgtaggag gtggcggcga ggtcgcgggc cttccggctg cggaggttcg tgtagaagac
     4681 gaagccctcg gggccgaagc cccggagcag cacattgcgg gccgagggcc ggccgtcggc
     4741 cccggcggtg gccacgacca tgacgtcggc ctcctcgccc agcagggtgc gggcctcctc
     4801 ccaccagcgg cggaaccgct ccatcgggtc cgatggagcg
//
LOCUS       DASUTW010000349         2993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1156674, whole genome shotgun sequence.
ACCESSION   DASUTW010000349 DASUTW010000000
VERSION     DASUTW010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156674"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..762
                     /locus_tag="VF058_11935"
     CDS             64..762
                     /locus_tag="VF058_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6209052.1"
                     /translation="MSDPTSPILEARKVVKTYHTGAADVQALRGLDLTVHPGEMVAVV
                     GPSGSGKTTLLNCLSGLDEIDEGEVFIAGEEIHSMPDRVRTSFRGREMGFVFQAFNLI
                     QVFTAAENVELPLLLNGVPPKEARRRAESVLELVGLGHRLSHRPPELSGGEQQRVAIA
                     RALAASPSIVWADEPTGNLDSVTAEQVMTLLRRLNEDGLTVILVTHDLAIARTASRIV
                     SMHDGQIVGDEPQP"
     gene            759..893
                     /locus_tag="VF058_11940"
     CDS             759..893
                     /locus_tag="VF058_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6209053.1"
                     /translation="MRWRRPLTGQACRRRPPWLGLALVAVLLVAAALALPGLLPGTLP
                     "
     gene            914..>2993
                     /locus_tag="VF058_11945"
     CDS             914..>2993
                     /locus_tag="VF058_11945"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6209054.1"
                     /translation="MAILAGLLGLLAIVTAARHAVPRRLALRSIARRPGETVLVVLGS
                     LLGTAIITGSFIVGDTLDSSIRASAETQLGPIDETVTGFGTESLPALRRALEGLEDEP
                     RVDGVTFAIRAAGTAAARIDTDDVAVQPRLLVLEMDLEEAGSFGGDPAATGLDGAATP
                     AEGEAVITEDLAAHLEVGRGDEITLFAYGSERELRVTGLVPRLGVAGYTTEFSAESFN
                     VFVAPGTLEELVAAAPPDLEASPPTSLAFVSNEGGVFEGEEPTDEVVPLIRERIAGLE
                     GVDVVDSKRDVVEAADRSGSQFSELFLSIGSFAVIAGILLLVNIFVMLSEERKGELGM
                     MRAVGMRRGQLVRSFYIEGSLYGLVASAIGAVAGIGVGAAIVRVAADIFSGAGEFSLE
                     LVLSAPLVSIVGGFLIGLAISLLTALFTSARISRLNIIRAIRDLPEPPVRRRRLRGLV
                     LGAVAVVVGGGMTAFAISEGEAASAFAGPGILALGLGALLWRLVPRRPLGSVLGLAVL
                     GWGIFGPTLAPDVFGEADIPVFVIQGVLLTASAVAVLGFNQESVGHAVRAVAGGARNL
                     AARLGLAYPLAKRFRTVMTLSMYALVVFTLVFISVLSNIFGGQVEDFTRQEAGGYDLL
                     VRSSAANPLTAEAAAAEDGVEAASPIRYAAFSVEFSTELQPEAEPWALSGIEEGFLAQ
                     GPPALEKLAPE"
BASE COUNT          352 a         1083 c         1097 g          461 t
ORIGIN      
        1 ccgcgccgcc ccacacctcc atcccctccg tcgcggagcc cttcctgtag ggttgcctcc
       61 ggcatgagcg acccgacctc gccgatcctg gaggctcgga aggtcgtgaa gacctaccac
      121 acgggggcgg ccgacgtgca ggcgctgcgc ggcctcgacc tcaccgtgca ccccggcgag
      181 atggtggccg tggtcggccc ctccgggtcg ggcaagacca cgctcctcaa ctgcctctcg
      241 ggcctcgacg agatcgacga gggcgaggtc ttcatcgcgg gcgaggagat ccactcgatg
      301 cccgaccggg tgcgcaccag cttccgtggc cgcgagatgg gcttcgtgtt ccaggccttc
      361 aacctgatcc aggtgttcac ggcggccgag aacgtcgagc tgccgctgct gctgaacggg
      421 gtgccgccga aggaggcccg gcgccgcgcc gagagcgtgc tcgagctcgt cggcctcggc
      481 caccgcctga gccaccgccc gcccgagctc tccggaggcg agcagcagcg ggtggcgatc
      541 gcgcgggccc tcgcggcgag cccttcgatc gtgtgggccg acgagcccac ggggaacctc
      601 gacagcgtca ccgccgagca ggtgatgacg ctgctgcgcc gcctgaatga ggacggcctc
      661 acggtgatcc tcgtcacgca cgacctggcg atcgcgcgga cggcgtcgcg gatcgtctcc
      721 atgcacgatg gccagatcgt gggggacgag ccccagccat gaggtggagg cggccgctga
      781 cggggcaggc gtgcaggagg cgtccgccat ggctgggtct cgccctcgtg gccgtgctct
      841 tggtcgcggc tgccctggcg ctccccggcc tcctgccggg gaccctcccg tgaaccccgc
      901 gtacgttccg gcgttggcga tcctcgccgg cctcctgggc ctgctcgcga tcgtcaccgc
      961 ggcccgccac gcggtcccgc ggcgcctcgc gctgcgctcc atcgcccggc ggccggggga
     1021 gacggttctc gtcgtcctcg ggtcgctcct ggggaccgcg atcatcaccg ggtcgttcat
     1081 cgtcggggac acgctcgaca gctcgatccg ggccagcgcc gagacccagc tcggcccgat
     1141 cgacgagacg gtcacgggct tcgggaccga gtccctcccc gcgctccgcc gagcgctgga
     1201 gggcctcgag gacgagccgc gcgtggacgg cgtgaccttc gcgatccgtg ccgccggcac
     1261 cgccgccgcc cgcatcgaca ccgacgacgt cgccgtccag ccccgcctgc tcgtcctgga
     1321 gatggacctc gaggaggccg gctcgttcgg cggcgacccc gccgcgacgg gcctcgacgg
     1381 cgcggcgacg cccgccgagg gcgaggccgt catcacggag gacctcgccg cgcacctcga
     1441 ggtcgggcga ggcgacgaga tcaccctctt cgcctacggg agcgagcgcg agctgcgcgt
     1501 caccggtctc gtcccgcgcc tgggggtcgc cgggtacacc accgagttca gcgccgagag
     1561 cttcaacgtc ttcgtggctc ccgggaccct ggaggagctg gtggccgccg cgccgccgga
     1621 cctggaggcc tcgcccccca cgtcgctggc gttcgtatcc aacgagggcg gggtcttcga
     1681 gggcgaggag ccgaccgacg aggtcgtccc gctgatccgg gagcggatcg cgggcctcga
     1741 gggcgtggac gtcgtggaca gcaagcgcga cgtggtcgag gcggccgacc ggtcgggcag
     1801 ccagttctcg gagctcttcc tctcgatcgg atccttcgcc gtcatcgccg gaatcctgct
     1861 gctcgtcaac atcttcgtga tgctctccga ggagcggaag ggcgagctgg gcatgatgcg
     1921 ggcggtcggg atgcggcgag gccagctcgt gcgctccttc tacatcgagg ggtcgctgta
     1981 cgggctggtg gcgtcggcga tcggggcggt ggccgggatc ggtgtgggcg ccgccatcgt
     2041 gcgggtggcg gccgacatct tctcgggggc cggggagttc tcgctggagc tcgtgctgtc
     2101 ggcgccgctg gtcagcatcg tgggcggctt cctgatcggc ctggcgatct cgctcctgac
     2161 cgccctcttc accagcgccc gcatcagccg cctgaacatc atccgggcca tccgggacct
     2221 ccccgagccg cccgtccggc gccgccgcct gcgcgggctc gtgctgggag cggtggcggt
     2281 ggtggtcggg ggtgggatga ccgccttcgc catctccgag ggagaggcgg cctcggcctt
     2341 cgccggcccg ggcatcctcg ccctggggct gggtgccctg ctctggcggc tcgtcccgcg
     2401 acggccgctg ggctcggtcc tggggctggc ggtcctgggc tgggggatct tcgggccgac
     2461 gctggctccc gacgtcttcg gggaggccga catccccgtg ttcgtgatcc agggcgtgct
     2521 gctgacggcg tccgccgtgg cggtcctcgg cttcaaccag gaatcggttg gtcacgcggt
     2581 ccgcgcggtg gccgggggcg cccgcaacct cgccgcgcgg ctggggctcg cctacccgct
     2641 ggccaagcgc ttccgcacgg tgatgaccct ctcgatgtac gcgctcgtcg tgttcacgct
     2701 cgtgttcatc tcggtgctgt cgaacatctt cgggggccag gtcgaggact tcacccggca
     2761 ggaggcgggc ggctacgacc tgctggtccg gtcgtcggcg gcgaaccccc tgacggccga
     2821 ggcggccgca gccgaggacg gcgtggaggc cgcatctccc atccgctacg cggccttcag
     2881 cgtggagttc agcacggagc tccagcccga ggccgagccg tgggcgctct cggggatcga
     2941 ggagggcttc ctggcccagg gtcccccggc gctggagaag ctcgcccccg agt
//
LOCUS       DASUTW010000350         7369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1548077, whole genome shotgun sequence.
ACCESSION   DASUTW010000350 DASUTW010000000
VERSION     DASUTW010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7369
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548077"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..2645)
                     /locus_tag="VF058_11950"
     CDS             complement(108..2645)
                     /locus_tag="VF058_11950"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HEX6209055.1"
                     /translation="MREALEEPGAAEAAVRLLGFSGPAADFLVAYPEEAGLFSDLGPR
                     SREALLQESRADVGRMGGEAGLRRFRRRALLRVAARDLGGAALEEVVEEVTSVAEVCL
                     ALAAEAARAGALAVIGMGKLGGEELNYASDVDVIFVHEDGPPEEAARAASRLIALLSE
                     PTRDGVALRVDAALRPEGRAGPLSRSLASTVEYYARHAATWERQALLKARPVAGDLEL
                     GARLLRELEPVIYPDHLDPEAIEDVRAVKARLEDHVRARGKEETEVKRGRGGIRDVEF
                     AVQLLQLVHGRRDARLRSRGTLPALAALAEQGYVAPGDAEALAGSYRFLRTVEHRLQI
                     ARDLQTHELPEDRPSRRRLARSLGIASPAELRAAYERHTEVVRGLHERLFYRPLLEVF
                     AGPRVPRPAEERAQTEELLTALGFREPPAAYERLDRLMDADTRRGRVLEGVFPVVAPG
                     LSAAPDPDRALVRFDRVAERLDEEGADLLAADPTVAWRLALLVGASGWAADFLAAQPE
                     AAALLDGRDRPDARPRERLLAAAGSYVAGDLRVPALGRALARIAEGVVAEALRAAQPD
                     LPLAVIALGKLGGEELNFGSDLDVVFVYDGEGEEPFREAVRVAEATLEGIREAGYEPD
                     PDLRPEGKNGPLARSMASFLEYWQRWGQTWEFQSLLKARAVAGEESLGRRWLSNAEDL
                     AYPEALPLDREAEIRRMRVRMEKERVRPPEAARFHFKLGHGGLADVTFAVELAQMRRG
                     RRHPAVRRRHTLEALEALASEGLIEDGVALALGEAYVFLNEVKNAMELDARRPAEALP
                     PTPEAQVALARLLGYEEHPRQAFLEEYRRVTRRARRAMERVFYGEEP"
     gene            complement(2741..4051)
                     /locus_tag="VF058_11955"
     CDS             complement(2741..4051)
                     /locus_tag="VF058_11955"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX6209056.1"
                     /translation="METEQVLAEVEERGIRFVRLWFTDVLGFLKSFSIPIEELEKAFR
                     EGIGFDGSAIEGFARVEEADMLARPDPLTFRVLPVPREEPAARMLCDITYPDGAPFEG
                     DPRHVLRRNLSRLEGRSFHAEPEVEFFLSGSEERFQPLDHGSYFDLTASDAAEEFRRR
                     AVPVLETLGIEIRMLHHEDAPGQQEIDLAPGDALRVADDLITLRLLVKEVAQELGLHA
                     SFMPKPAPGIQGSGMHVHYRLLEGERNAFFDPTDEARLSKEGRSFVAGLLEHAPAMTA
                     VTNQWVNSYKRLVPGFEAPIHVSWARRNRSALVRVPADRPDDEAACRIEYRGADPACN
                     PYLAFAVVLAAGLDGIERGLEAPPEVYEDLASLSDDELRALGVRSLPDNLHDAIAEME
                     RSDLLAEALGERLFEWLIRNKREEWEVYRTYVTPFEVERYFPLL"
     gene            4157..5485
                     /locus_tag="VF058_11960"
     CDS             4157..5485
                     /locus_tag="VF058_11960"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX6209057.1"
                     /translation="MTVTKEAEFVLRTVEERRIRFIHLWFTDVLGFLKSFAITPAELE
                     TAFTEGMGFDGSSIEGYSRIQESDMLALPDPTTFQVIPWRSEEGVARMYCDIVNPDGT
                     PFDGDPRWVLKRQLQRAADLGFTFYVGPELEYFYFTDDRGTEFLDRGGYFDLTPRDVA
                     TDYRKRTVGYLEAMGIPVEYVHHEVAPSQHEIDLRYTDALTMADNVMTYRLTVKETAQ
                     EFGVHATFMPKPVAGVNGSGMHTHQSLFEGDRNAFFDGTDEYHLSKTAKGYIAGILRH
                     APEITLVLNQWVNSYKRLVPGYEAPVYVCWARRNRSALVRVPMYKPGKEAATRIELRS
                     PDPACNPYLAFAAMLAAGLKGIEEEYELPPEASNNIYEMIEEERREAGIEHLPEDLHD
                     AIREAEESELLRETLGDHVHEFLIRNKREEWDAYKRYVTPYELERYLPVL"
     gene            5505..7130
                     /locus_tag="VF058_11965"
     CDS             5505..7130
                     /locus_tag="VF058_11965"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX6209058.1"
                     /translation="MRIALGQLNTTVGDLEGNVERLAAGAVEAAGQGADLVVFPELAI
                     TGYPPEDLLLRPAFVEDNLEALEELARRAPDATSVLVGFVDRTDLGMHNAAALLRGGR
                     IEARYHKHKLPNYGVFDEARYFVPGEEGCALRVADAELGISICEDAWSPGPPFDGYAQ
                     AVQIIPNLNASPYHRGKIGERVEICADRARETGTWIVYVNAVGGQDELVFDGGSMVVA
                     PNGERAWHADMFEEDMLVVDLPEASEGKPPLPDPEREPWPEGPDEVYRALVLGLGDYV
                     RKNDFTDVVLGLSGGIDSSLTAVLAVDALGPEHVWVLAMPSRFSSDHSLEDAEELAER
                     LAIRLDRVSIDDTFQAYLDALAPIFEATEPGVAEENVQARIRGNLLMAASNKFGHLVL
                     ATGNKSEMAVGYSTLYGDMAGGFAPLKDVPKTLVYELARWRNEQTDPVVPERVLTKPP
                     SAELRPDQQDTDSLPPYEVLDPIVEAYVEEDRSPEEIVADGADPALVDRVVRMIDGSE
                     YKRRQAAPGVKITPKAFGRDRRLPITNRYRRAP"
     gene            7139..7212
                     /locus_tag="VF058_11970"
     tRNA            7139..7212
                     /locus_tag="VF058_11970"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7173..7175,aa:Lys,seq:ttt)
BASE COUNT         1101 a         2736 c         2558 g          974 t
ORIGIN      
        1 gccacgctcc acgacccgga acccggggga cggagcggca ccgagcccgc cggccccgtc
       61 ctcggcgcag gccacggccg tcaaggcgag gatcagcgcg gcggtcctca tggctcctcc
      121 ccgtagaaga cccgctccat cgcccggcgg gcccggcggg tgacccgacg atactcctcc
      181 aggaaggcct gccgggggtg ctcctcgtat cctaggaggc gggccagcgc cacctgcgcc
      241 tccggcgtgg gcggcagggc ctcggccggc cgccgcgcat cgagctccat cgcgttcttc
      301 acctcgttga ggaagacgta ggcctcgccg agggccagcg ccacgccgtc ctcgatcagc
      361 ccctcggagg ccagggcctc cagtgcctcc agcgtgtgcc ggcggcggac ggcggggtgc
      421 cggcggccgc gccgcatctg ggccagctcg acggcgaagg tcacgtcggc cagccccccg
      481 tggccgagct tgaagtggaa gcgcgccgcc tcggggggcc gcacgcgctc cttctccatc
      541 cggacccgca tcctccggat ctcggcttcc cggtccaggg gaagcgcctc agggtaggcc
      601 aggtcctcgg cgttggatag ccagcgccgg cccagcgact cctcccccgc caccgcccgg
      661 gccttcagca gcgactggaa ctcccaggtc tggccccacc gctgccagta ctccaggaac
      721 gaggccatgg agcgggccaa cgggccgttc ttcccctcgg gccggagatc ggggtcgggc
      781 tcgtagcccg cctcccggat cccctccagc gtggcctcgg cgacgcggac ggcctcgcgg
      841 aacggctcct cgccctcccc gtcgtagacg aagaccacgt ccaggtccga gccgaagttc
      901 agctcctcgc cccccagctt gcccagggcg atcaccgcga ggggaaggtc gggctgggcc
      961 gcccgcaggg cctccgcaac gacgccctcg gcgatccgcg ccagggcccg acccaaggct
     1021 ggcacgcgga ggtcgccggc gacgtaggat cccgcggccg ccaggagccg ctcccggggc
     1081 cgggcgtccg gacgatcgcg cccgtcgagc agggccgcgg cctcgggctg ggcggccagg
     1141 aagtcggccg cccagcccga ggcccccacc agcagcgcca gccgccacgc caccgtcgga
     1201 tcggcggcca gcaggtcggc cccctcctcg tccagccgct cggccacgcg atcgaaccgc
     1261 acgagcgccc tgtcgggatc gggggcggcc gagaggccgg gcgccaccac cgggaagacc
     1321 ccctccagca cccggccccg ccgcgtgtcc gcgtccatca gacggtccag ccgctcgtag
     1381 gcggccggag gctcccggaa gccgagggcg gtcagcagct cctcggtctg ggcccgctcc
     1441 tccgccgggc gcggcacgcg cggtcccgcg aacacctcca gcagcgggcg gtagaacaag
     1501 cgctcgtgga gcccgcgcac gacctccgtg tggcgctcgt aggcggccct gagctcggcg
     1561 ggcgacgcga tgcccagcga ccgggccagc cgccgccggg acggccggtc ctccggcagc
     1621 tcgtgcgtct ggaggtcccg ggcgatctgg agccggtgct ccacggtccg caggaagcga
     1681 tacgagcccg ccagcgcctc ggcgtcgccg ggggccacgt acccctgctc ggccagggct
     1741 gccagcgcgg ggagggtgcc gcgcgatcgg agcctcgcgt cccgtctccc gtgcaccagc
     1801 tggaggagct gcacggcgaa ctccacgtcc cggatcccgc cccgtccccg cttgacctcg
     1861 gtctcctcct tgcctcgggc ccgaacgtga tcctcgagac gggccttcac cgcccgaacg
     1921 tcctcgatcg cctccgggtc gaggtggtcc gggtagatca ccggttccag ctcgcggagc
     1981 aggcgggcgc ccagctcgag gtccccggcc acgggccgcg ccttgagcag ggcctgccgc
     2041 tcccaggtcg ccgcgtggcg cgcgtagtac tcgacggtcg aggcgagcga gcgcgagagg
     2101 gggccggccc gtccctcggg gcgcagggcg gcgtccaccc tgagggcgac cccgtcccgc
     2161 gtgggctccg agagcagggc gatgagccgc gacgcggccc gggccgcctc ctccggcggc
     2221 ccgtcctcgt ggacgaagat cacgtcgacg tccgaggcgt agttcagctc ctcgcctccc
     2281 agcttcccca tcccgatcac ggccagggcg ccggcgcggg cggcctccgc ggccagcgcc
     2341 agacagacct ccgcgaccga ggtcacctcc tcgacgacct cctccagcgc ggcgccgccc
     2401 aggtcgcggg ccgcgacgcg cagcagcgcc cgccgccgga accgccggag gccggcctcg
     2461 cctcccatcc gccccacgtc cgcccgggac tcctggagca gcgcctccct ggagcgcggg
     2521 cccaggtcgg agaagagccc cgcctcctcc gggtacgcca cgaggaagtc cgcggcgggc
     2581 cccgagaacc cgagcaggcg gaccgccgcc tccgccgcgc ccggctcctc cagggcttcc
     2641 cgcacccgcg ggtcctcccc aacgcgcgac agcgcgaccc gtgccatctc ggggtcgggg
     2701 gccgcggcga gcgcccggcc gaggtcctcg ggtgagcgca ctagaggagg gggaagtagc
     2761 gctcgacctc gaacggggtg acgtaggtgc ggtagacctc ccactcctcg cgcttgttgc
     2821 ggatcagcca ctcgaacaat cgctcgccca gcgcttcggc cagcaggtcg gagcgctcca
     2881 tctcggcgat cgcgtcgtgg aggttgtcgg ggagtgagcg gaccccgagc gcgcggagct
     2941 cgtcgtcgga gagcgaggca agatcctcgt agacctcggg cggcgcctcg agcccacgtt
     3001 cgatcccgtc gagtccggcc gccagcacca ccgcgaaggc caggtacggg ttgcacgccg
     3061 ggtcggcccc ccggtactcg atccggcatg cggcctcgtc gtcgggccgg tcggccggca
     3121 ctcggaccag cgcggaccgg ttccgccggg cccacgacac gtggatcggc gcctcgaacc
     3181 cggggacgag ccgcttgtag ctgttcaccc actggttcgt gacggcggtc atggccggcg
     3241 cgtgctccag caggcccgcc acgaacgacc gcccctcctt cgacagcctg gcctcgtcgg
     3301 tcgggtcgaa gaacgcgttc cgctcgccct ccagcagacg gtagtggacg tgcatccccg
     3361 agccctggat cccgggcgcc ggcttgggca tgaacgacgc gtggagcccc agctcctggg
     3421 ccacctcttt gacgagcaga cggagcgtga tcaggtcgtc ggccacgcgg agggcgtccc
     3481 ccggcgccag gtcgatctcc tgctggcccg gggcgtcctc gtgatggagc atgcggatct
     3541 cgatcccgag cgtctccagg acggggacgg cccggcgccg gaactcctcg gccgcgtcgc
     3601 tcgccgtgag gtcgaaatag gatccgtggt ccagcggctg gaaccgctcc tccgacccgg
     3661 agaggaagaa ctcgacctcg ggctcggcgt ggaacgaccg cccctcgagc cgagacaggt
     3721 tgcggcggag gacgtgtcgg gggtcaccct cgaacggcgc cccgtccggg taggtgatgt
     3781 cgcagagcat cctcgccgcc ggctcctccc gcgggacggg gagcacccgg aacgtcaggg
     3841 ggtccggccg ggccagcatg tcggcctcct cgacccgggc gaaaccctcg atcgccgagc
     3901 cgtcgaagcc gatcccctca cggaaggcct tctccagctc ctcgatgggg atggagaacg
     3961 acttcaggaa gcccaggacg tccgtgaacc agagccgcac gaaccggatg ccccgctcct
     4021 cgacctcggc caggacctgc tcggtctcca cgcggccatc gtaaaccggg cgctccgcgc
     4081 acgaccgatc cgcgccgcaa cccaaggttt acgcagccgt aaccacggcg agtcgtcgac
     4141 cgcctaccct ggccgcatga cggtgacgaa ggaagcggag ttcgtcctgc gcaccgtgga
     4201 ggagcgcagg atcaggttca tccatctgtg gttcaccgac gtgctggggt tcctcaagtc
     4261 gttcgcgatc acgcccgccg agctcgagac cgcgttcacc gaagggatgg gcttcgacgg
     4321 gtcctcgatc gaggggtact cgcggatcca ggagtcggac atgctcgcgc tccccgaccc
     4381 cacgaccttc caggtgatcc cctggcgctc cgaggagggc gtcgcgcgga tgtactgcga
     4441 catcgtgaac cccgacggca cgccgttcga cggcgacccc cgctgggtgc tgaagcggca
     4501 gctccagcgg gcggccgacc tcgggttcac cttctacgtg ggccccgagc tcgagtactt
     4561 ctacttcacc gacgaccggg ggaccgagtt cctcgaccgc ggcggctact tcgacctcac
     4621 cccgcgcgac gtggccaccg actaccgtaa gcggaccgtc gggtacctcg aggcgatggg
     4681 catccccgtg gagtacgtcc accacgaggt ggcgcccagc cagcacgaga tcgacctccg
     4741 ttacaccgac gcgctgacga tggccgacaa tgtcatgacc taccggctca cggtcaagga
     4801 gacggcgcag gagttcggcg tgcacgccac gttcatgccg aagccggtgg ccggcgtgaa
     4861 cggcagcggg atgcacacgc atcagtccct tttcgaaggc gaccgcaacg cgttcttcga
     4921 cggtaccgac gagtaccacc tgtcgaagac ggcgaagggc tacatcgcgg ggatcctgcg
     4981 gcacgccccc gagatcacgc tcgtgctgaa ccagtgggtg aactcctaca agcggctcgt
     5041 gcccgggtac gaggcgccgg tctacgtctg ctgggcgcgg cgcaaccggt cggcgctggt
     5101 ccgggtgccc atgtacaagc cgggcaagga ggcggccacg aggatcgagc tccgctcccc
     5161 cgacccggcc tgcaacccgt acctcgcgtt cgcggcgatg ctggccgccg gcctcaaggg
     5221 gatcgaggag gagtacgagc ttccgcccga ggcctcgaat aacatctacg agatgatcga
     5281 ggaggagcgg cgcgaggccg gcatcgagca ccttccggag gacctccacg atgcgatccg
     5341 cgaggcggag gagtccgagc tgctgcggga gaccctgggc gaccacgtcc acgagttcct
     5401 gatccggaac aagcgcgagg agtgggacgc ctacaagcgc tacgtcactc cctacgaact
     5461 ggagcgctac ctgcccgtcc tctgagcggg cctatgctgt ggccatgcgc atcgcgctcg
     5521 gccagctgaa cacgacggtc ggcgacctgg agggcaacgt cgagcgcctc gcggccggcg
     5581 ccgtcgaggc cgcgggccag ggcgccgacc tcgtcgtctt ccccgagctc gcgatcaccg
     5641 gctacccgcc cgaggacctc ctcctgaggc ccgccttcgt cgaggacaac ctcgaggccc
     5701 tggaggagtt ggcgcgccgc gcgcccgatg cgacctcggt gctcgtcggc ttcgtggatc
     5761 ggaccgacct tgggatgcac aacgcggcgg ccttgctccg tggcgggagg atcgaggccc
     5821 gctaccacaa gcacaagctg cccaactacg gcgtcttcga cgaggcccgc tacttcgtcc
     5881 cgggcgagga gggctgcgcc ctgcgggtcg ccgacgcgga gctcgggatc tcgatctgtg
     5941 aggacgcgtg gagccccggg ccgccgttcg acggctacgc gcaggccgtc cagatcatcc
     6001 ccaacctcaa cgcctccccc taccaccgag gaaagatcgg ggagcgcgtc gagatctgtg
     6061 ccgaccgggc ccgcgagacc gggacctgga tcgtctacgt caacgcggta ggcggccagg
     6121 acgagctcgt cttcgacggg ggcagcatgg tcgtggcgcc gaacggcgag cgagcctggc
     6181 acgcggacat gttcgaggag gacatgctcg tcgtggacct gcccgaggcg tccgagggga
     6241 agccgccgct ccccgatcct gagcgcgagc cctggccgga gggaccggac gaggtctacc
     6301 gcgcgctcgt gctcggcctc ggagattacg tgcggaagaa cgacttcacc gacgtggtcc
     6361 tggggctgtc gggcgggatc gactcctcgc tgacggcggt gctggcagtc gacgcgctgg
     6421 ggcccgagca cgtctgggtc ctggcgatgc cctcccgctt ctcctcggac cacagcctcg
     6481 aggacgcgga ggagctggcc gagcgcctcg cgatccggct ggaccgcgtg tcgatcgacg
     6541 acacgttcca ggcctacctc gacgcgctag cgccgatctt cgaggcgacg gagccgggtg
     6601 tggccgagga gaacgtccag gcccggatcc gcggcaacct cctgatggcg gcctcgaaca
     6661 agttcgggca cctggtgctg gctaccggca acaagagcga gatggcggtg gggtactcga
     6721 cgctgtacgg ggacatggcg ggcgggttcg cgccgctgaa ggacgtgccg aagacgctcg
     6781 tgtacgagct ggcgcggtgg cggaacgagc agacggaccc cgtcgtcccc gagcgggtgc
     6841 tgacgaagcc gccctccgcg gagctccgac cggaccagca ggacaccgac agcctcccgc
     6901 cgtacgaggt cctcgacccg atcgtggagg cctacgtgga ggaggaccgg tcacccgagg
     6961 agatcgtggc ggacggtgcc gaccccgcgc tcgtggatcg cgtggtgcgg atgatcgacg
     7021 gatcggagta caagcggcgc caggccgcgc ccggggtgaa gatcacgccg aaggccttcg
     7081 ggcgcgaccg ccgcctcccc atcacgaacc gctaccgccg cgcgccgtag aatcgtctgg
     7141 ggcgttagct cagtctggca gagcagtggg cttttaaccc atcggtcggg ggttcgaatc
     7201 cctcacgccc cacaaatcgc gacactaccg atcggttccc ctcgacccgg cgggcgacat
     7261 cgcatctatc gcgtgtgcga gaccgcggcg acccgcccac gatccaccgc ttccctccgc
     7321 cacgcgggca acagatatac atcgctgcga gattgcgtgg ccggcccgc
//
LOCUS       DASUTW010000351         6055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_249984, whole genome shotgun sequence.
ACCESSION   DASUTW010000351 DASUTW010000000
VERSION     DASUTW010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6055
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249984"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..785)
                     /locus_tag="VF058_11975"
     CDS             complement(129..785)
                     /locus_tag="VF058_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX6209059.1"
                     /translation="MRERGSVSIVVAVAMGMAAISAALMGDLGAVASGVARTQAAADA
                     AALAAAQELVLPSLESPEEAAERFAALNGARLIRCRCPPGGEDVVVLVERSVPLPFLG
                     GTRTVRRAARATAALGGDVAGLEPWFLSRLACLRARVPDLWVVSGFRTGAEQARLHRE
                     RPGLAAPPGRSLHELGLAADLGFGSASVGALAHREAPTCGLVFPLSHEPWHVEPAGGD
                     "
     gene            complement(782..1216)
                     /locus_tag="VF058_11980"
     CDS             complement(782..1216)
                     /locus_tag="VF058_11980"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX6209060.1"
                     /translation="MGPLLRSPPGVLRPHHRSDPRRGGVAGSATVEFALVLPLVALVV
                     AGLVQVARLGGESLTVLSAAREAARTAAVTVDDGEVRRAVIASGLDEARTDILVERGG
                     GLGTPVTVSVSYRTPAGPPILSWLLPAEVTLRARATMRQEVG"
     gene            complement(1141..1365)
                     /locus_tag="VF058_11985"
     CDS             complement(1141..1365)
                     /locus_tag="VF058_11985"
                     /inference="COORDINATES: protein motif:HMM:NF025395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HEX6209061.1"
                     /translation="MLRIWTAVVSWWVVLRARLLDRLGGSRGQTTAEYALVILAAAAI
                     AIGLLAWARSSDRLPAFFDRIIDRILGEAE"
     gene            complement(1375..2187)
                     /locus_tag="VF058_11990"
     CDS             complement(1375..2187)
                     /locus_tag="VF058_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX6209062.1"
                     /translation="MIVVGLGYAAAAALLAAAAASRSPVPARLGLRPRPRRDLLQALG
                     RRVPWTPPVLAERLGILARPADVDRLRGAAAVCALMGLIGGTLVTAPSPAAVGVGILA
                     AVAGWRLPAFTLARRAASARGHMMPEVAALLDLVTVGLSAGLTPRLAFDRAVDHVAEP
                     LREALVRVRREVALGASWPASLDHLGRRFALPDLRRLASTLDQSIRLGAPVADPMRSL
                     ARDVRAERDAAVEARARRAPVTMLFPLVLCILPAFVLAAVLPAMLVATRGVP"
     gene            complement(2184..2981)
                     /locus_tag="VF058_11995"
     CDS             complement(2184..2981)
                     /locus_tag="VF058_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX6209063.1"
                     /translation="MAGPVVTGLAAGASILAAAAERRRMSRRIESPVDPTGGRAAALL
                     GAGLCSAAAGLLLFGPLASLLACGGLVGWGLIHRAKAREAEALETERGLRDAVRALAA
                     SARAGLSIRRSLEEVARGGPAAVAGPFRRAAGELVMGRPLPAALASLSDGLGSREAAM
                     LTGVLSIHHRAGGDLPTALDEVAALAERRAEARRRVRALTAQGRASGAVLALLPVAFV
                     GLLSGTSGNGLGTIYASPRGAALLGAGLVLQLVGYAWIRAILRRAQP"
     gene            complement(2987..4216)
                     /locus_tag="VF058_12000"
     CDS             complement(2987..4216)
                     /locus_tag="VF058_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX6209064.1"
                     /translation="MTLLERVAARVRERLQGESLVGRSPAERRLRVREEALAVLREER
                     AVLAARELGELVALVSDELTGLGPIEHLLRDPDVSEVMVNGPGAVYVERKGRLELVGG
                     GLFEGEEAVYHLIERVVGPLGLRADESCPYVDARLPDGSRFHAIIPPLSLCGPVVTVR
                     KFAPSPYGPEDLLRLGTLEERILEVLETCVRGRANILISGGAGSGKTTLLGVLSSFIP
                     AGERIVTIEDAAELRLRFPHVVRLEARPASAEGRGAVTVRDLVRNALRMRPDRIVVGE
                     VRGGEALDMLQAMNTGHEGSMSTVHANSPQELMARLETMALMADVVLPADHLREHIAQ
                     TVDLVAHMVRLREGRRILARLTSVEGPVGGRPLLRDLFRFAGGGWETLGSPAGIALRQ
                     GRDELDLLGISPNGTAR"
     gene            complement(4213..5292)
                     /locus_tag="VF058_12005"
     CDS             complement(4213..5292)
                     /locus_tag="VF058_12005"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6209065.1"
                     /translation="MDGTTVVLGFDAPALQEQVLRFIERRPGLRIAGAAGDAQALTSK
                     VRETGPQVVITTPDVLAAGPELNGAALLVVGERETTAGLRAAIRAGARGFYLWPEERD
                     SLGHDADRAGADPERPDGRVVAVYGPRGGVGTTFLATNLAAACAGDGRRVILADLDLH
                     FADVTTALGIVANGESRTIADVAALADDLGASHLEAVLHRHPRGFEILLAPPRAEQAA
                     DVEPEQVRAMGKALRSAGDLIVLHVPRALDRVTLAALEAADEILLVVTLDVLAFRDAK
                     RAMELLAAKDLSERCRLVVNRARRAEVLPDDAERVFGLRPVAVIASDPAVPRAQDRGQ
                     IVVGRRGQAGRKVAALARSILKEGS"
     gene            5639..5995
                     /locus_tag="VF058_12010"
     CDS             5639..5995
                     /locus_tag="VF058_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6209066.1"
                     /translation="MISRILIAVLAFAFGAAMPLSALGGERQEAVVREEDLWEETAYS
                     LDDDDDSDGNSGATSFTSGVNSNDKTNSRHTPVTRDRDRSRGDLTRDRTKDGPGKSKR
                     DWTKNRTNDRSRNDTR"
BASE COUNT          918 a         2334 c         2152 g          651 t
ORIGIN      
        1 gctccccgga tcctctccaa caagcccttg aggcccacgc gacctcgctc ccaatcggcc
       61 tccgacgcgc acttgccgcg acgcatgacc tggtcggcgg ccagcatagg gggaaccgtt
      121 cgccacggtc aatccccgcc ggccggctca acgtgccacg gctcgtgact cagggggaag
      181 acgagcccgc aggtcggtgc ctcccgatgg gccagcgcac cgacagacgc agatccgaag
      241 cccaggtccg cggccaggcc cagctcgtgg agggatcgcc cgggcggcgc agccagcccg
      301 ggcctctccc gatgcagccg cgcctgctcg gccccggtcc ggaacccgga gacgacccag
      361 agatcgggca cgcgggctcg cagacacgcc aggcgagaga ggaaccaggg ctccagcccg
      421 gccacgtcgc cccccagggc cgcggtagcc ctggccgccc ggcggacggt ccgcgtcccc
      481 cccaggaacg gcagcggcac gcttcgctcc acgagcacga cgacgtcctc gcctcccggt
      541 gggcagcgac accggatcaa ccgagcgccg ttgagggccg cgaaccgctc cgctgcctcc
      601 tcggggctct cgagggacgg gagcacgagc tcctgggcgg cggccagcgc ggccgcgtcg
      661 gccgcggcct gcgtgcgcgc cacgcccgac gcgacggccc ccagatcgcc cataagggcc
      721 gccgagatcg ctgccatgcc catcgccacc gcgacgacga tcgagacgct tccccgctcc
      781 ctcacccgac ctcctgtcgc atcgtggccc gggcccggag ggtcacctcg gcggggagaa
      841 gccacgaaag gatcggcggg cccgccggcg tgcggtagga gacgctcacg gtcacggggg
      901 tgccgagtcc gcctccgcgc tccacgagga tgtcggttcg ggcctcgtcg agtcccgaag
      961 cgatcacggc tcgccggact tccccgtcgt cgacggtgac ggccgcggtc cgagcggcct
     1021 cgcgcgcggc cgagagcacc gtcagcgact cgccgcccag acgggccacc tgcacgagac
     1081 ccgccaccac cagagccacc agcggaagca cgagagcgaa ctcgaccgtc gcgctcccgg
     1141 ctactccgcc tcgccgagga tccgatcgat gatgcggtcg aagaacgccg ggaggcgatc
     1201 ggaggagcgg gcccaggcca gaagcccgat cgcgatcgcg gccgcggcca ggatcaccag
     1261 ggcgtactcg gcggtggtct gtcccctgct ccccccgaga cgatccagga gacgggctcg
     1321 aagaacgacc caccaggaga cgacagcggt ccagatcctg agcatggccc ctcctcatgg
     1381 gacgcctcgg gtggcgacga gcatcgcggg gagcaccgcc gccaggacga acgcgggaag
     1441 gatgcacagc acgaggggga agagcatcgt caccggagcc cgacgggctc gcgcctccac
     1501 ggccgcgtcg cgctccgccc ggacgtcgcg ggccagcgat cgcatcgggt cggccaccgg
     1561 ggccccgagc cggatcgact gatccagcgt cgaggccagg cgccggaggt cggggagggc
     1621 gaagcgacgt cccaggtggt ccagggaagc gggccagctc gcgccgaggg cgacctctcg
     1681 gcggacccgc accagcgcct cccggagcgg ctcggccacg tggtccaccg cccgatcgaa
     1741 ggccagccgg ggcgtcagcc ccgccgagag ccccaccgtg accaggtcca gcagggcggc
     1801 gacctccggc atcatgtgcc ctcgggcgga ggcagcccgg cgggcgagcg tgaaggcggg
     1861 gagccgccag ccggccaccg ccgccaggat tccgaccccg acggcggccg gcgacggagc
     1921 cgtgacgagg gtcccgccga tcagacccat cagggcgcac acagccgcgg cgccccggag
     1981 ccggtccacg tctgcggggc gggccaggat ccccagacgc tccgccagca cgggaggcgt
     2041 ccacgggacg cggcggccca gggcctggag aaggtccctc cgaggccgag gtcgaaggcc
     2101 cagccgcgcg ggcacggggc tgcgactcgc ggcggccgcg gccaggaggg ccgcggccgc
     2161 cgcgtacccc agaccgacca cgatcacggc tgggccctcc tgaggatcgc ccggatccac
     2221 gcgtagccga cgagctgcag cacaagcccc gcgccgagca gcgccgcgcc ccgggggctg
     2281 gcgtagatgg tgccgaggcc gttcccactc gtcccggaga ggaggccgac gaaggcgacc
     2341 ggaaggagag cgagaacggc cccggacgct cgaccctgcg cggtgagggc ccgaacccgg
     2401 cggcgggcct ctgcccgccg ctccgccagg gccgcgacct cgtccagggc ggtcgggagg
     2461 tcgcccccgg cacgatgatg gatcgacagg acgccggtca gcatggcggc ctcgcgcgat
     2521 cccagtccat cggagaggct tgcgagcgcc gcggggagcg ggcggcccat caccagctcc
     2581 ccagccgccc gccggaacgg ccccgcgacg gcggcggggc ccccgcgtgc gacctcctcg
     2641 agcgagcgac ggatcgacag gccggcccga gccgacgccg cgagcgcgcg aaccgcgtcc
     2701 ctgagccctc gctcggtctc gagagcttca gcctcccgcg ccttcgcacg gtggatgagc
     2761 ccccacccca cgagccctcc gcaagcgagc agcgaggcga gcggaccgaa cagcaggaga
     2821 ccggcggcgg cgctgcacag ccccgcgccc aacaacgccg ccgcgcggcc accggtcgga
     2881 tccacgggag attcgatccg gcgggacatg cgcctccgct ccgcggccgc ggccaggatc
     2941 gacgctccgg cggcgagccc cgtcaccacg ggtccggcca cctcgatcat cgggccgtcc
     3001 cgttggggga gatgccgagc aggtccagct cgtcgcggcc ctggcggagc gcgatgccgg
     3061 ccggcgagcc gagcgtctcc cagccgcccc ccgcgaagcg gaagaggtct cgcagcaggg
     3121 gtctgccgcc caccggcccc tccaccgagg tcagccgggc caggatcctg cgcccctcgc
     3181 ggaggcgcac catgtgggcg acgaggtcga cggtctgggc gatgtgctcg cggaggtggt
     3241 ccgcgggcag cacgacgtcg gccatcagcg ccatcgtctc cagccgcgcc atcagctcct
     3301 ggggcgagtt ggcgtggacc gtggacatcg agccctcgtg ccccgtgttc atcgcctgga
     3361 gcatgtcgag ggcctctccc ccgcgaacct cccccacgac gatgcgatcg ggtctcatcc
     3421 gcagcgcgtt ccggacgaga tcccggaccg tgacggctcc ccgcccctcc gcggaggccg
     3481 gccgggcctc gaggcggacc acgtgcggga accggagcct cagctccgcg gcgtcctcga
     3541 tggtcacgat gcgctccccg gccgggatga aggaggacag cacgccgagg agcgtggtct
     3601 tcccggaccc cgcgccgccc gagatcaaga tgttggctcg accccgcacg caggtctcca
     3661 ggacctccag gatccgttcc tccaaggtcc ccagccgcag gagatcctcg ggcccgtacg
     3721 gcgagggcgc gaacttccgg accgtcacca cgggaccgca gagcgaaagg ggcgggatga
     3781 tcgcgtggaa ccgcgagccg tccggaagcc gggcgtccac gtacgggcac gactcgtcgg
     3841 cccggaggcc cagcggcccg accacgcgct cgatcaggtg gtagacggcc tcctccccct
     3901 cgaacaggcc gccgcccaca agctcgagcc gccccttccg ctccacgtac acggcccccg
     3961 ggccgttcac catgacctcg gacacgtcgg ggtctcgcag gaggtgctcg atcggaccga
     4021 ggccggtcag ctcgtcggat accagcgcga cgagctcgcc gagctcccga gccgcgagga
     4081 ccgcccgctc ctcccggagg acggcgagcg cctcctctcg gacgcgcagc ctccgctccg
     4141 cgggcgaccg gcccacgagc gactcgccct ggaggcgctc ccgcacccgc gcagccaccc
     4201 gctcaaggag cgtcacgatc cctcctttag gatcgaccgg gccagcgccg caacctttcg
     4261 tccggcctgg cctcgccgcc cgaccacgat ctgtccccga tcctgcgcgc gcgggacggc
     4321 cgggtcggag gcgatgaccg cgaccggccg gaggccgaag acccgctccg cgtcgtccgg
     4381 taggacctcg gccctccggg ctcggttcac cacgaggcgg cagcgctcgg agaggtcctt
     4441 cgccgcaagg agctccatcg cccgcttggc gtcgcggaac gccagcacgt ccagggtcac
     4501 gacgaggagg atctcgtccg ccgcttcgag ggcggccagc gtcacccgat cgagggcgcg
     4561 cggtacgtgg aggacgatca ggtccccggc gctgcgaagg gccttcccca tggctcgcac
     4621 ctgctcgggc tcgacatcgg ccgcctgctc ggcccggggc ggagcgagga ggatctcgaa
     4681 gcccctcggg tggcggtgga gcaccgcctc gaggtgggag gcgccgaggt cgtcggcgag
     4741 ggccgccacg tccgcgatcg tccgggactc gccgttggcc acgatgccga gcgccgtggt
     4801 gacgtcggcg aagtgaaggt ccaggtcggc taggatcacg cgccggccgt cgccggcgca
     4861 ggccgccgcc aggttcgtgg ccaggaaggt cgtgcccacg ccgccccggg ggccgtacac
     4921 ggcaaccacc ctgccgtcgg ggcgctccgg gtccgctccc gctcggtccg cgtcgtgtcc
     4981 gagcgagtcc cgctcctcgg gccagaggta gaagccgcga gcccccgcgc ggatcgctgc
     5041 gcgcagcccg gcggtggtct cacgctcccc gaccaccagg agcgcggcgc cgttcagctc
     5101 ggggccggcg gccagcacgt cgggcgtcgt gatcacgacc tggggcccgg tctcgcggac
     5161 cttgctcgtc agggcctggg cgtccccggc cgcgcccgcg atccggagac ccggtcgccg
     5221 ctcgatgaag cggagcacct gctcctggag ggccggcgca tcgaagccca gcaccaccgt
     5281 cgtcccgtcc atccgctcct cctgtcaaca cttcggggaa ggagggggag ggggtgcgcg
     5341 gaccgcctgg gagggggcgg tccgcgtgca ggagacggta ccgagccagc gcggcacgac
     5401 gcatcgcccg gaacggggag atgctcggtg gattccccgc tcccccgggc ggcgcgccct
     5461 cccccacctg gcgatgcccc gcccccatac ggaggatgtg gcactccgcc gtcgtcgccg
     5521 acaccggtgc cctgccccag acgccacctc tcgtgagaag accctcaccc tcctcccacc
     5581 gaggtctcat gcccgggccc cacactgggg ccatgttcgg aggacgaagg aggagcagat
     5641 gatctcgagg atcctgatcg ccgtcctggc gttcgcgttc ggggccgcga tgccgctctc
     5701 cgcgctcggc ggggagcgcc aggaggccgt ggtgcgggag gaggatctct gggaggagac
     5761 cgcctacagc ctggacgacg acgacgacag cgacggcaac tccggcgcca cgagcttcac
     5821 cagcggcgtc aacagcaacg acaagacgaa cagccggcac acgcccgtga cccgcgaccg
     5881 tgaccggagc cggggggacc tcacccgcga ccggacgaag gacggccccg gcaagagcaa
     5941 gcgggactgg acgaagaaca ggacgaacga ccgctcccgg aacgacaccc gctgagcggg
     6001 cgtcgctcta gggctccggc tcgaccggct cgatcccgag ccggtcgagc cgtct
//
LOCUS       DASUTW010000352         2514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_250195, whole genome shotgun sequence.
ACCESSION   DASUTW010000352 DASUTW010000000
VERSION     DASUTW010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250195"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..111)
                     /locus_tag="VF058_12015"
     CDS             complement(<1..111)
                     /locus_tag="VF058_12015"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEX6209067.1"
                     /translation="MQLGRVIGTVVATEKAEGLEGIRFLIVQPLDRRKEPS"
     gene            complement(111..710)
                     /locus_tag="VF058_12020"
     CDS             complement(111..710)
                     /locus_tag="VF058_12020"
                     /inference="COORDINATES: protein motif:HMM:NF013129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HEX6209068.1"
                     /translation="MDPALALLEFDSIARGIEAGDAMIKRSPLQVLRAGTVHPGKYLV
                     LVGGMTADVEEAMDAGREVAGPDLVDLVYLPDVHPHVVTAIGGERREDPGEALGVVET
                     RTVAASILAADAGVKAAAVVLREVRLADGLGGKGYVLLGGPVAEVEAAVEAALARIEA
                     TDKALAHVVIARLHEGMEENLAAEPRFLPLAEGLEKEPA"
     gene            complement(713..2158)
                     /locus_tag="VF058_12025"
     CDS             complement(713..2158)
                     /locus_tag="VF058_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6209069.1"
                     /translation="MISQDEIQGIIERVKARLGAGDGAALRGGEELAASEEQPFGDGI
                     FPTIGEAVAAAARAFDEYKAMGLEGRKRVVASIRASMLEHGERLAEMAASETGLGRAE
                     DKAIKNRLVTTKTPGPEDLEVEAATGDRGMVATEWAPFGVVASITPTTNPTSTIINNT
                     ISIVSAGNALVFNVHPNAKRVSAENIRLLDRAIVAAGGPPNLVTGIPEPTIDSAKELM
                     HHPDVRLLLVTGGPGVVEEALKTDKRAVTAGPGNPPAVVDETADVEKAARDIIAGASL
                     DNNIICTDEKTTIVVDAVGDRLVRAMAKAGAYVLKEHELRRLERVIFRELGEPGKPGK
                     INPGWIGKNAGAILAEIGIRVDDAVRLAVAEVPNEHSLVWTEQMMPVMPVTRVPNVER
                     AIDLAVRSEHRFRHTASIHSTNVDTITKMARAMNCSIFVANGPNYAGLGEGGEGFTSF
                     SIASPTGDGLTRPRTFSRERRVTVVGALRIV"
     gene            complement(2155..>2514)
                     /locus_tag="VF058_12030"
     CDS             complement(2155..>2514)
                     /locus_tag="VF058_12030"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEX6209070.1"
                     /translation="SGVGRGLAAAHPPSGKEGTVLLGRVAGTLVATRKEPTMEGLKFL
                     VVRSLDVDGEETGQSVVAADAVGAGVGEVVMLAQGSSARQTEATRDRPCDAVIMAIVD
                     QWEVGGEERYHKYRDAS"
BASE COUNT          316 a          959 c          849 g          390 t
ORIGIN      
        1 ggacggctcc ttccgtcggt ccagcggctg gacgatgagg aagcggatgc cctcgaggcc
       61 ctcggccttc tcggtggcca cgaccgtccc gatcacgcgg cccagctgca tcaggccggc
      121 tccttctcca ggccctcggc cagcgggagg aaccggggct cggccgcgag gttctcctcc
      181 atgccctcgt ggagtcgggc gatcacgacg tgggccaggg ccttgtccgt ggcctcgatc
      241 cgcgccaggg cggcctcgac ggccgcctcc acctcggcca cggggcctcc gaggagcacg
      301 tagcctttcc cgccgagccc gtcggccagg cgcacctcac gaagcacgac ggccgcggcc
      361 ttgacgccgg cgtcggccgc gaggatcgat gccgccacgg tcctcgtctc cacgaccccc
      421 agggcctcgc ccgggtcctc gcgccgctcg cccccgatcg cagtcacgac gtgcgggtgg
      481 acgtcgggca ggtagacgag gtccacgagg tcgggtccgg ccacctctcg tcccgcgtcc
      541 atcgcctcct cgacgtcggc ggtcatccca cccacgagca cgaggtactt ccccgggtgc
      601 acggtgcccg cccgcaggac ctggagcggc gaacgcttga tcatcgcgtc gcccgcctcg
      661 atccccctcg cgatcgagtc gaactcgagg agggcgaggg ccggatccat cgtcagacga
      721 tcctcagggc gccgaccacc gtgacccggc gctcgcgcga gaacgtccgc ggccgcgtca
      781 ggccgtcccc ggtggggctg gcgatcgaga acgaggtgaa cccctcgccc ccctccccca
      841 gcccggcgta gttgggcccg ttcgccacga agatcgagca gttcatggcc cgcgccatct
      901 tcgtgatcgt gtccacgttc gtggagtgga tcgaggcggt gtgccggaac cggtgctcgg
      961 accgcacggc gaggtcgatg gcccgctcca cgttgggcac gcgcgtcacc ggcatcacag
     1021 gcatcatctg ctcggtccac acgaggctgt gctcgttcgg gacctcggcc accgcgagcc
     1081 gcacggcgtc gtcgacgcgg atcccgatct cggccaggat cgccccggcg ttcttgccga
     1141 tccacccggg gttgatcttc cccggctttc cgggctcccc cagctcgcgg aagatcaccc
     1201 gctccaggcg ccgaagctcg tgctccttca gcacgtaggc tccggccttc gccattgccc
     1261 ggaccaggcg atccccgacc gcgtccacga cgatcgtcgt cttctcgtcg gtgcagatga
     1321 tgttgttgtc gagcgaggca cccgcgatga tgtcgcgcgc cgccttctcc acgtcagcgg
     1381 tctcgtccac gacggccggc gggttgcccg ggcccgcggt caccgcgcgc ttgtcggtct
     1441 tcagcgcctc ctcgacgacg ccgggcccgc cggtgaccag cagcaggcgg acgtcgggat
     1501 ggtgcatcaa ttccttggcg ctgtcgatcg tgggctccgg gatcccggtg acgagattgg
     1561 gcgggccgcc cgccgccacg atcgcgcggt cgagcaggcg gatgttctcg gccgagaccc
     1621 gcttcgcgtt ggggtggacg ttgaacacga gcgcgttccc cgcggacacg atcgagatcg
     1681 tgttgttgat gatcgtcgag gtcgggttcg tggtcggcgt gatcgaggcc acgacgccga
     1741 acggggccca ctcggtggcc accatgcccc ggtccccggt ggcggcctcg acctcgaggt
     1801 cctcgggccc gggcgtcttc gtggtcacga ggcggttctt gatcgccttg tcctcggcac
     1861 gccccagccc ggtctcggag gcggccatct cggccagccg ctcgccgtgc tccagcatcg
     1921 aggcccggat cgaggccacg acgcgcttgc ggccctccag gcccatcgcc ttgtactcgt
     1981 cgaaggcgcg ggcggccgcg gccacggcct cgccgatcgt ggggaagatg ccgtcgccga
     2041 agggctgctc ctcggacgcg gcgagctcct ccccgccccg gagcgccgcc ccgtcgccgg
     2101 ccccgagccg ggccttgacc cgctcgatga tgccctggat ctcgtcctgg gagatcacga
     2161 ggcatcacgg tacttgtggt accgctcctc cccgccgacc tcccactggt cgacgatcgc
     2221 catgatcacc gcgtcgcacg ggcggtcgcg ggtggcctcg gtctggcggg cggagctgcc
     2281 ctgggcgagc atcacgacct cgcccacgcc ggccccgacg gcgtcggcgg ccaccaccga
     2341 ctggcccgtc tcctcgccgt ccacgtcgag ggagcgcacg acgaggaact tcagcccctc
     2401 catcgtgggc tccttgcggg tggccacgag cgtcccggcc acgcgtccca gcagcacggt
     2461 gccctccttc ccggaagggg ggtgggcggc ggcgaggccg cggcccaccc ccga
//
LOCUS       DASUTW010000353         2195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_382698, whole genome shotgun sequence.
ACCESSION   DASUTW010000353 DASUTW010000000
VERSION     DASUTW010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382698"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2195)
                     /gene="rpoC"
                     /locus_tag="VF058_12035"
     CDS             complement(<1..>2195)
                     /gene="rpoC"
                     /locus_tag="VF058_12035"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019851643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX6209071.1"
                     /translation="VDARRDEQIQARLKDLEARIKELEGEGAKKAQLTAANKEAQREI
                     DKMTEAARGETEHLEEVLGAFKSLHVKQLVADDSVYRTLRDRFGDYFEGGMGAEAIRE
                     LLAGLDLDEEAENLRETIKTAKGTRRQKAIKRLKVVSSFIGTKNSPMGMVLDAIPVIP
                     PDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLQAPEIIVNNEKRMLQEAVD
                     ALFDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPNLK
                     LHQCGLPKQMALELFKPFVMKQLVDRGLAQNIKSAKRMVERARPQVWDVLEDVIKDHP
                     VMLNRAPTLHRLGIQAFEPVLVEGKAIQIHPLVCPAFNADFDGDQMAVHLPLSSEAQS
                     EARILMLSTNNILSPAHGRTIASPTQDMVLGIYYLTASDEKPEKPEDIPSYTGVAEVL
                     MALDRGTVGVHDWVRVRLPGKNIPGAEENGHVPRLETTAGRVLFNEALPLDYPYVNDH
                     VMKGEVSRIVDECVRTYPRSEMGRILDNLKELGFQYATRAGLTIGVDDVTTPQSKQKI
                     LDGYEREAQKVESQYRKGIITDDERRQELIEIWTRATDEVRDEMEASFDKFNPIFMMA
                     HSGARGNITQIRQIAGMRGLVANPRGEIIPRPIKSNFREGLTVLEYFIATHGARKGLA
                     DTALRTADSGYLTRRLVDVAQEVIVREDDCGTDKGMTLEVTYEGPDGER"
BASE COUNT          280 a          765 c          730 g          420 t
ORIGIN      
        1 cccgctcgcc gtccggaccc tcgtaggtca cctccagggt catccccttg tccgtcccgc
       61 agtcgtcctc ccggacgatg acctcctgcg cgacgtcaac gagccggcgg gtcaggtacc
      121 cggagtcggc cgtccgcagg gccgtgtccg ccagcccctt ccgggctccg tgtgtcgcga
      181 tgaagtactc gagcacggtc agcccctcgc ggaagttcga cttgatcggg cgcgggatga
      241 tctcgcccct cgggttggcc acgaggcccc gcatccccgc gatctggcgg atctgagtga
      301 tgttcccgcg ggcccccgag tgggccatca tgaagatggg gttgaacttg tcgaagctcg
      361 cctccatctc gtcgcggacc tcgtcggtgg cccgcgtcca gatctcgatc agctcctggc
      421 gccgctcgtc gtcggtgatg atccccttcc ggtactgcga ctcaaccttc tgggcctcgc
      481 gctcgtagcc gtcgaggatc ttctgcttcg actggggggt cgtcacgtcg tccacgccga
      541 tcgtgagccc ggcccgcgtc gcgtactgga acccgagctc cttgaggttg tcgaggatcc
      601 ggcccatctc gctccgcggg taggtccgca cgcactcgtc gacgatcctg ctgacctcgc
      661 ccttcatcac gtggtcgttg acgtaggggt agtcgagcgg cagggcctcg ttgaacagca
      721 cgcgcccggc ggtcgtctcc aggcggggaa cgtggccgtt ctcctcagcc cccgggatgt
      781 tcttgccggg caggcgcact cggacccagt cgtggacgcc gacggtcccc cggtcgagcg
      841 ccatcagcac ctcggccacg cccgtgtagc tcgggatgtc ctccggcttc tcgggcttct
      901 cgtcggacgc cgtcaggtag tagatcccga ggaccatgtc ctgggtcggc gaggcgatcg
      961 tgcgcccgtg ggccggcgac aggatgttgt tcgtggacag catcaggatc cgggcctcgg
     1021 actgcgcctc ggaggagagc ggcaggtgca ccgccatctg gtcgccgtcg aagtcggcgt
     1081 tgaaggccgg gcagacgagg gggtggatct ggatcgcctt gccctcgacc agcaccggct
     1141 cgaacgcctg gatccccaga cggtgcaggg tcggcgcccg gttcagcatc accgggtgat
     1201 ccttgatgac gtcctcgagc acgtcccaga cctgcggccg cgcccgctcc accatgcgct
     1261 tcgcgctctt gatgttctgg gccaggccgc ggtccacgag ctgcttcatc acgaacggct
     1321 tgaagagctc gagggccatc tgcttgggga gcccgcactg gtgcagcttg aggttggggc
     1381 cgaccacgat caccgagcgg cccgagtagt ccacgcgctt ccccagcagg ttctggcgga
     1441 accggccctg cttgcccttc agcatgtcgc tcagggactt cagcggacgg ttgcccgggc
     1501 cggtgaccgg ccggccgcgg cggccgttgt cgaagagcgc gtccacggcc tcctgcagca
     1561 tgcgcttctc gttgttgacg atgatctcgg gggcctgcag gtcgagcagc cgcttcagcc
     1621 ggttgttccg gttgatcacg cgccgataga ggtcgttgag atcgctcgtg gcgaaccggc
     1681 cgccgtcgag ctgcaccatg gggcgcaggt cgggcgggat caccgggatc gcgtcgagca
     1741 ccatccccat cggggagttc ttcgtgccga tgaagctgga gaccaccttc agccgcttga
     1801 tcgccttctg gcgtcgggtt cccttcgccg tcttgattgt ctcccggagg ttctcggcct
     1861 cctcgtcgag gtcgaggccg gcgagcagct cgcggatcgc ctcggcgccc atgccgccct
     1921 cgaagtagtc cccgaagcgg tcccgcaggg tccggtacac ggagtcgtcg gccacgagct
     1981 gcttcacgtg gaggctcttg aaggcgccca ggacctcctc gaggtgctcg gtctcgccgc
     2041 gggccgcctc ggtcatcttg tcgatctccc gctgggcctc cttgttggcc gcggtgagct
     2101 gggccttctt ggcgccctcg ccctccagct ccttgatccg ggcctcgagg tccttcagcc
     2161 gggcctggat ctgctcgtcg cgacgcgcgt cgacg
//
LOCUS       DASUTW010000354         7716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1418020, whole genome shotgun sequence.
ACCESSION   DASUTW010000354 DASUTW010000000
VERSION     DASUTW010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7716
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418020"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="VF058_12040"
     CDS             <1..1133
                     /locus_tag="VF058_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6209072.1"
                     /translation="RIRGAKSVSHASFIIPPDRAIGETTHLDRPFVPEETYLEIRLSQ
                     MYLRNEREYFREFSPLASLMTELLYAGGRRTVPVVVGPSLLSEAEPVPSGDGVEFFNT
                     RVAGPYPYAGDDVDLFAGLFRAEVDNWAERALSLLETVAKALDLTKLSSFVNVSQPLV
                     DGVEGLLRMEEMELRLGVDRSYRTPSESGQGSNTLRPGYEVLLRIPSDRLSEKDQGRF
                     WVRDGRLLHGEDAASAVPFTDADFLLLEIGCLDVRHDYTTFDFHREHWKKVEEHIWAG
                     RESDAKDRFKLLAASLVQSEDIVRPHRRRLLRTYKDKLDQEIAEYRELFEAAESGEKA
                     FEGRAPEPIREEELQAVARSSGEPIPEGGPEEMLSLLEGGPS"
     gene            complement(1130..4147)
                     /locus_tag="VF058_12045"
     CDS             complement(1130..4147)
                     /locus_tag="VF058_12045"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6209073.1"
                     /translation="MDRRDDSADEGRDAAGMPPRNTTSEIPTPSADIPASLGEKGGGQ
                     WRALRRAGAVDRSGLEAELEVARQDLGAGRLQEVVRRLEPMLLRMSQAEPALDAVLGS
                     AFSVLGRALRGLGRDDDAAAAFGRAVERLDRSGPDPEIPGAAHDHAVALIAVGRRPQG
                     IAAMRAAIDAQVASGQMVPAGAYTELAAALQEDGAVEEAERTYRAALEVDPDDPEAQI
                     ALGGSLAARGEDAAAATFLRDAAWDLGAANRLPEALEAVDRSLSLAPGDPYAFGIRGE
                     LLRMMGDAPGAIASLREALQGEPDNLVWQASLGAALAATGELDEARRLLERVVGEEPD
                     YAFAQANLGDVLRVQGRFDEALRAADRALEIEPTNIIALATKAAALWGSGRSEGALEF
                     ADRALDLNPDYVFALGVRARILQALERSEEAIRDLEHLVGVEPSAPARAQMAAGLVLG
                     GRNEEGLAMVEPLLLEHPDDAHLQWVKGVALKNLGRFQEAAAAFESSLRTAPDNAELL
                     ADYALCLQSLERREEAVEALDRAVSLRPDQAYYWALKADLSWALSRHEEALQAADRAL
                     AVDPTLVMAFVTRAQSLLALGRPEDALGAIDEALELNPDYGYAAGVKGQILRASGEDK
                     EAVSTLYRAMELDPSLTWAVADLASALGGLGRYEEALDVIDRAVREAPDDPQLLGLRA
                     EILRLLERHEEALADADRALVLRPDYDFVLGTKGQTLVTMGRAEEALPVLDKALEIDP
                     EYLFALIAKASALWNLGRLEEGIQVTDRALEIDPRSVAALSTRAGILMDAADFEGGLR
                     SLDRAVEIDPEDAYTAGLRGIALLGLRDGREREAEEAFRTAIATSPDLHWKVGLGDAV
                     RIRSGSEAADAIYREAIADAEERSAPDANTMSLLAWCHYRLREYPESARLSARALGLD
                     PTMISAQFDLGLALMVGGDHDAAVSEYERGLHLTEDVASPLRRRGLLANAAAYLQEAV
                     TEEPGLSESDAARKVLGLLEQEIERGPGSPAVL"
     gene            complement(4157..5605)
                     /locus_tag="VF058_12050"
     CDS             complement(4157..5605)
                     /locus_tag="VF058_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6209074.1"
                     /translation="MTEPDAFMLKTRLDNLRRNLEDVEHVVKSVGEQRSVFHGLLPLP
                     RTVRGALAEVGSRVDLILGALRREDMTTAEQEIERLGARVTELTVRMGEQAAADEVLS
                     EVERRFNIVQLSDKEELGSVLVDAAKDQKEKLAEVEKRVRSAEEKAAQGKGEEANQLL
                     DEAWNTYATQLSASTDRLFSEYVDFLGGLALRDSGFDRGICSIADELIRQAPKIGGGW
                     SSLTIPAHHEALNMTLARIIRLGFPEWTVWTLPLTAHELGHVLVAETGDAFADVEDDR
                     TAQVCLADAYGTLVMGPAYACAAMLIRLNPLTAFHPEDDRLTAKRAETVLRTLDHMNA
                     WDEVDPPYAQVRQGLEAAWAEGTGHAEPSGELGAEELETLEAWIERMSGWLGSYRALP
                     GEHWPRIKRWAQALGTEKQDEIELQNDDEHRFVLNAAWQARLDHPEQTEHIARDALAL
                     WRRLDEHLRKETGGGKGQAQAGSFPHSGQHTT"
     gene            complement(5602..6723)
                     /locus_tag="VF058_12055"
     CDS             complement(5602..6723)
                     /locus_tag="VF058_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6209075.1"
                     /translation="MDGLEQVDDNLHGRALDAIGDALDRHARDLASLRELARSDGDGA
                     WDKLRELRRDSSTTFRESLAFMQGALARREGLDRGLCRIADSMLLEVSGRARVAWNRL
                     TILDQAEFVQDLSQIIRIRFPEVSVWSLPIATHELGHFVAPRIEVQVQEGKVRRLAYP
                     FQELLENLLDRDRERGATEPERFRSWSFMHEHIADVFAVVAGGPGYVCATILHRFDPS
                     TAGTDTLQHPSGAKRAHMILRTLAEMDRAPGLLGEYTGLIDHLRGTWARSVSSAGGTP
                     ELAPEDEKCINEIGDELYGLFQDHAASLLYRTVLRAKELRTNLLSGEQPAPADGDTVT
                     DAVNAAWLARVGSGDDGSIGDLGERAFRLCEAIVGEEGR"
     gene            complement(6942..>7716)
                     /locus_tag="VF058_12060"
     CDS             complement(6942..>7716)
                     /locus_tag="VF058_12060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6209076.1"
                     /translation="RHAEELGLDMVTISDHLHGVRTWEPWTVLTWIAAGTSRIRVSPN
                     VLGLPYRHPAVIAKMAETLHRLSEGRVVLGIGAGGSDAEFEAFGLPVRSPGEKVDAFE
                     EALRIIRGLWEEETFSFRGEHYAVEEARIEPRPGHIPIWIGAYGDRMIELTGRYADGW
                     LPSLMYVSLDRAAGKMEQLRAAAEAAGRDPAEITMAVNVWARIEDGAEPRRGQIAGGV
                     DRVVEETARVLEAGFDHLNFTVSGEEQRARLAEEVLPGL"
BASE COUNT         1028 a         2874 c         2638 g         1176 t
ORIGIN      
        1 agaggatccg gggagcgaag agtgtctccc acgcgagttt catcatcccc cccgatcggg
       61 cgatcggcga gactacgcac ctcgaccgtc ccttcgtccc cgaggagacc tacctcgaga
      121 tccgtctctc ccagatgtac cttcggaacg agcgagagta ctttcgcgag ttcagccccc
      181 tcgcctccct gatgactgag ctcctctacg cgggcgggcg gaggaccgtc cccgtcgtcg
      241 tgggaccctc gctgctgtcg gaagcggagc cggtcccgtc gggtgatggg gtcgagttct
      301 tcaacacgcg cgtggccggc ccgtacccgt acgccgggga cgatgtggac ctcttcgccg
      361 ggctgttccg ggccgaggtc gacaactggg cggaacgggc gctctcgctc ctcgagacgg
      421 tggccaaggc gctcgacctc acgaagctca gctcgttcgt caacgtgagc cagcccctgg
      481 tcgacggggt ggagggcctc ctgaggatgg aggagatgga gctccggctc ggcgtcgacc
      541 ggagctaccg cacgccctcc gagtccggcc aggggtccaa cacgctccgc ccgggctacg
      601 aggtcctgct ccgcatcccg tcggatcggc tgagcgaaaa ggatcagggg aggttctggg
      661 tccgcgacgg ccggctgctg cacggcgagg atgcggcctc cgccgtcccc ttcaccgacg
      721 ccgacttcct gctcctggag atcggctgtc tcgacgtccg ccacgactac accaccttcg
      781 acttccatcg ggagcactgg aagaaggtcg aggagcacat ctgggcgggg cgggagagcg
      841 atgcgaagga ccgcttcaag cttctcgcgg ccagcctcgt gcagtccgag gacatcgtcc
      901 ggccgcatcg tcgccgcctt ctgcgcacct acaaggacaa gctcgatcag gagatcgcgg
      961 agtaccgcga gctgttcgag gccgccgagt cgggcgagaa ggcgttcgag gggcgagcgc
     1021 cggagccgat ccgcgaggag gagctccagg ccgtcgcgcg ctcctcgggt gagccgatcc
     1081 ccgaaggagg gcccgaggag atgctgtccc tgctggaggg cggaccctcc tagaggaccg
     1141 ccggagagcc cgggcctcgc tcgatctcct gctcgagcag tcccagcacc ttcctcgccg
     1201 cgtcgctctc cgagaggccg ggctcctctg tgaccgcttc ttgtaggtag gcggcggcgt
     1261 tggccaggag cccccgccgc cgcagagggc tggccacgtc ctcggtcagg tgcaggccac
     1321 gctcgtactc gctcacggcc gcgtcgtggt cccccccgac catgagcgcg agccccagat
     1381 cgaactgcgc ggagatcatc gtcggatcca ggccgagcgc gcgcgccgag agccgcgccg
     1441 actccgggta ctcgcgcagc cgatagtggc accaggcgag aagcgacatg gtgttggcgt
     1501 cgggggccga ccgctcctcc gcgtccgcga tcgcctcgcg gtagatcgcg tccgcagcct
     1561 cggaccccga gcggatgcgc accgcgtcgc cgagccccac cttccagtgc aggtccggcg
     1621 acgtcgcgat cgccgtgcgg aacgcctcct ccgcctctcg ctcgcgcccg tcccgcaagc
     1681 cgagcagcgc gatccctcgc agccccgcgg tgtacgcgtc ctccgggtcg atctcgacgg
     1741 ctcggtcgag ggagcgcagc cctccctcga agtcggccgc gtccatcagg atccccgcgc
     1801 gggtcgagag cgccgccacc gatcggggat cgatctccag ggcgcggtcg gtgacctgga
     1861 tcccctcctc gagtcgtccc aggttccaca gcgcgctcgc cttggcgatg agggcgaaga
     1921 ggtactcggg gtcgatctcc agcgccttgt cgagcaccgg gagcgcctcc tcggctcgcc
     1981 ccatcgtgac gagggtctgg cccttggtcc ccagcacgaa gtcgtaatcc gggcggagga
     2041 cgagcgctcg atcggcgtcg gccagcgcct cctcgtgtcg ctccaggagc ctcaggatct
     2101 cggccctgag accgaggagc tgcgggtcgt cgggcgcctc gcgcacggcc cgatcgatca
     2161 cgtccagcgc ctcctcgtag cgccccagcc cgcccagggc cgaggcgaga tcggccacgg
     2221 cccaggtgag cgaggggtcg agctccatcg cccggtacag ggtcgacacc gcctccttgt
     2281 cctctccgga ggcgcgaagg atctgcccct tcacgcccgc ggcgtagccg tagtcggggt
     2341 tgagctcgag ggcctcgtcg atcgccccca gggcgtcctc gggccgcccc agggcgagca
     2401 gcgactgggc cctggtcacg aacgccatca cgagcgtcgg gtcgacggcg agcgcccgat
     2461 ccgccgcctg cagcgcctcc tcgtgccgcg agagcgccca cgacaggtcg gccttcagcg
     2521 cccagtagta cgcctggtcc ggccgcaggg acacggctcg gtcgagcgcc tcgacggcct
     2581 cctcccgccg ctccaggctc tggaggcaga gcgcgtagtc cgcgagcagc tccgcgttgt
     2641 cgggcgcggt ccgaagcgac gactcgaagg ccgcggcggc ctcctggaag cgccccaggt
     2701 tcttgagcgc gacccccttg acccactgga gatgcgcatc gtccggatgc tccaggagga
     2761 ggggctcgac catggcgagg ccctcctcgt tccggcctcc cagcaccagc ccggcggcca
     2821 tctgagcccg agcgggcgcg gagggctcga ccccgacgag gtgctccagg tctcggatcg
     2881 cctcctcgga ccgctcgagc gcctgcagga tgcgggcccg aacgccgagc gcgaacacgt
     2941 agtcggggtt gaggtcgagg gcgcggtcgg cgaactcgag ggccccctcc gatcggcccg
     3001 acccccagag cgccgccgcc ttggtcgcga gcgcgatgat gttcgtcggc tcgatctcca
     3061 gagcccggtc cgccgcacgg agcgcctcgt cgaaccgtcc ctggacccgg aggacgtccc
     3121 ccaaattcgc ctgggcgaac gcgtagtcgg gttcctcacc gaccacgcgc tcgaggagcc
     3181 gtcgggcctc gtcgagctcg ccggtcgccg cgagcgcggc ccccaggctg gcctgccaga
     3241 cgaggttgtc cggctcgccc tggagggcct cccggaggga tgcgatggcg cccggcgcgt
     3301 ctcccatcat ccgaaggagc tccccgcgga tgccgaaagc gtaggggtct cccggggcga
     3361 gcgacagcga acggtccacg gcctcgagcg cctcggggag gcggttcgcc gcccccaggt
     3421 cccacgccgc gtcccggagg aacgtcgcgg cggcggcatc ctcgccgcgc gcggcgaggc
     3481 tgccgccgag agcgatctga gcctccgggt cgtcgggatc gacctccagg gcggctcggt
     3541 acgtgcgctc ggcctcctcg acggcgccgt cctcctgcaa agccgccgcg agctccgtgt
     3601 acgcgccggc cggcaccatc tgtcccgagg cgacctgggc gtcgatcgcc gctcgcatcg
     3661 cggcgatgcc ctgcggtcgc cggccgaccg cgatgagggc gaccgcgtgg tcgtgggccg
     3721 ctccggggat ctccgggtcg ggtccggatc ggtcgaggcg ctccacggct cgcccgaagg
     3781 cggcggccgc atcgtcgtcc cgccccagcc cgcgcagggc tcgcccaagg acgctgaagg
     3841 ccgacccgag cacggcgtcc agcgcgggct cggcctggga catcctcagg agcatcggct
     3901 ccagccgccg caccacctcc tggaggcggc cggcgcccag gtcctgccgc gccacctcca
     3961 gctccgcctc gaggccggat cgatccacgg cgccggcgcg ccgcagggcc cgccactggc
     4021 cgccgccctt ctcaccgagg ctggcgggga tgtcggcgga cggcgtgggg atctccgacg
     4081 tcgtgttccg gggcggcatc cccgcggcgt cccggccctc gtccgcgctg tcgtctcgcc
     4141 gatccacggt gctctcctac gtcgtgtgct ggcccgagtg ggggaacgac ccggcctggg
     4201 cctggccctt cccgcccccg gtctccttcc gcaggtgctc gtcgagccga cgccacagcg
     4261 ccagcgcgtc ccgcgcgatg tgctcggtct gctcggggtg atcgagccgc gcctgccacg
     4321 cggcgttcag cacgaagcga tgctcatcgt cgttctgcag ctcgatctcg tcctgcttct
     4381 ccgtcccgag ggcctgggcc cacctcttga tgcgcggcca gtgctccccg ggcagggcgc
     4441 ggtagctccc cagccatccg ctcatccgct cgatccaggc ctccagggtc tcgagctcct
     4501 cggcgccgag ctctcccgac ggctcggcgt gtcccgtccc ctcggcccag gccgcctcga
     4561 gcccctgcct tacctgcgcg tacggggggt cgacctcgtc ccatgcgttc atgtggtcga
     4621 gggtcctcag gacggtctcc gctcgcttcg cggtcaggcg atcgtcctcg ggatggaagg
     4681 cggtgagggg gttcaggcgg atcagcatcg ctgcgcacgc gtaggccggg cccatgacga
     4741 gcgtcccgta cgcgtcggcg aggcagacct gggccgtccg gtcgtcctcc acgtcggcga
     4801 aggcgtctcc ggtctcggcg acgaggacgt gaccgagctc gtgggcggtg agcggcagcg
     4861 tccagacggt ccactccggg aaccccagcc tgatgatgcg agccagcgtc atgttgagcg
     4921 cctcgtggtg cgccgggatc gtcagggagc tccagccccc tccgatcttg ggcgcctgcc
     4981 tgatcagctc gtcggcgatg ctgcagatcc cgcggtcgaa gccggagtcc cgcagggcca
     5041 gcccgcccag gaagtccacg tactccgaga agagccggtc ggtcgaggcg gagagctggg
     5101 tcgcgtacgt gttccacgcc tcgtcgagaa gctggttcgc ctcctctccc ttcccctgcg
     5161 ccgccttctc ctccgcgctt cgaacccgtt tctccacctc cgccagcttc tccttctgat
     5221 ccttggcggc gtcgaccagg acgctaccca gctcctcctt gtcgctcagc tggacgatgt
     5281 tgaacctccg ctcgacctcg ctcaggacct cgtccgcggc ggcctgctcg cccatccgca
     5341 cggtcagctc tgtgacgcgc gcgccgagcc gctcgatctc ctgctcggcg gtggtcatgt
     5401 cctctcggcg gagcgccccg aggatgaggt ccacgcggct ccccacctcc gcgagcgcgc
     5461 cgcggaccgt gcgggggagc ggcaggaggc cgtggaagac ggagcgctgc tccccgacgg
     5521 acttcacaac gtgctccacg tcctcgaggt tccggcgtag gttgtcgagg cgcgtcttca
     5581 gcatgaaggc gtcgggttcg gtcaccttcc ttcctccccg acgatcgcct cgcagagacg
     5641 gaaggctcgc tctccgaggt cgccgatcga gccgtcgtcc ccggacccga cgcgggccag
     5701 ccacgcggcg ttcacggcgt cggtcaccgt gtcgccgtcg gcgggcgcgg gctgctcccc
     5761 cgacaggagg ttcgtccgca gctccttggc tcggaggacc gtgcgataga ggagcgaggc
     5821 ggcgtggtcc tggaagagcc cgtagagctc gtcgccgatc tcgtttatgc acttctcgtc
     5881 ctcgggggcg agctcggggg tgccgcccgc cgacgagacg cttcgggccc aggtgcctcg
     5941 aaggtggtcg atcagccccg tgtactcgcc caagaggccc ggggccctgt ccatctcggc
     6001 gagcgtccga aggatcatgt gcgctcgctt ggcgccgctc gggtgctgga gggtgtcggt
     6061 cccggcagtg ctggggtcga acctgtggag gatcgtcgcg cacacgtacc caggtccccc
     6121 ggcgacgaca gcgaagacgt ccgcgatgtg ctcgtgcatg aacgaccagg atcggaaccg
     6181 ctcgggctcg gtggccccgc gttcccggtc gcgatcgagg aggttctcca gcagctcctg
     6241 gaacggatac gcgagccgcc ggactttccc ctcctggacc tggacctcga tcctcggtgc
     6301 cacgaagtgc ccgagctcgt gggtcgcgat ggggaggctc cagacgctga cctccggaaa
     6361 gcggatccgg atgatctgcg agaggtcctg cacgaactcc gcctgatcga ggatcgtcag
     6421 gcggttccag gccaccctgg ccctcccgct gacctccagt agcatcgagt cggcgatccg
     6481 gcacaggccc cgatcgaggc cctcccggcg cgccagcgcg ccctgcatga acgcgaggct
     6541 ctcacggaag gtcgtgctgg agtcccggcg gagctcgcgg agcttgtccc acgccccatc
     6601 gccgtcgctc cgggcgagct ctcggagcga ggcgaggtct cgcgcgtgcc ggtccagggc
     6661 atccccgatc gcgtcgagtg cgcgcccgtg caggttgtcg tcaacctgct cgaggccgtc
     6721 catcacttcc tgtgcgcgag cgaactgggc ttccagcccg cggatctggg cggcgaggga
     6781 agtcgagccg tccgttctct cctccagcct tcgaggcgcc ggtgtacctg gctccgattc
     6841 gcagggcggc ggctccgtcg ggcagtggga gacgacgcca gccctgtggg cgcccgacca
     6901 ttgaagcgga tgggcgagat ccgcgcaata cccggtatcg cctagagccc ggggaggacc
     6961 tcctcggcca gcctggcccg ctgctcctcg cccgacaccg tgaagttcag gtggtcgaag
     7021 cccgcctcga gcacgcgcgc cgtctcctcg accacccgat ccacgccgcc cgcgatctgg
     7081 ccccggcggg gctccgcccc gtcctcgatc cgggcccaga cgttgacggc catggtgatc
     7141 tcggcggggt cgcggccggc ggcctcggcc gccgcccgga gctgctccat cttcccggcg
     7201 gcccggtcca gcgagacgta catcagcgag ggcagccacc cgtccgcgta gcgcccggtc
     7261 agctcgatca tccggtcgcc gtacgccccg atccagatcg ggatgtggcc cggccgcggc
     7321 tcgatccgcg cctcctccac ggcgtagtgc tcgccccgga acgagaacgt ctcctcctcc
     7381 cacaggccgc ggatgatgcg gagggcctcc tcgaacgcgt ccaccttctc gcccggggag
     7441 cgcaccggca ggccgaacgc ctcgaactcc gcatcgctcc cgccggcccc gatccccagt
     7501 acgacccgcc cctccgagag gcggtggagc gtctcggcca tcttcgcgat gaccgcgggg
     7561 tgccggtagg ggagccccag cacgttgggc gagacgcgga tccggctcgt gccggccgcg
     7621 atccaggtca ggaccgtcca cggctcccag gtccggaccc cgtggaggtg gtccgagatc
     7681 gtcaccatgt ccagcccgag ctcctcggca tgacgc
//
LOCUS       DASUTW010000355         7449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1031870, whole genome shotgun sequence.
ACCESSION   DASUTW010000355 DASUTW010000000
VERSION     DASUTW010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031870"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VF058_12065"
     CDS             complement(<1..586)
                     /locus_tag="VF058_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6209077.1"
                     /translation="MATEPLASRIRPRSLDEVVGQRHLLGPGKALAALVGADHLPSIV
                     LWGPAGTGKTTLAHLLAKAVGAELVQLSAVSSGVADARKVLESARGGMLQTVLFVDEV
                     HRWSKAQQDVLLPGVEAGDVTFIGATTENPYFSLVSPLLSRSLLLRLEPLTDGEVRTI
                     LERALMDERGLPGEKAEDEALAYLAETAGGDARLA"
     gene            complement(1092..2861)
                     /gene="aspS"
                     /locus_tag="VF058_12070"
     CDS             complement(1092..2861)
                     /gene="aspS"
                     /locus_tag="VF058_12070"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX6209078.1"
                     /translation="MADLRMRTHMCGGLGLEHAGERVALCGWVSTTRDHGGVIFVDLR
                     DREGEIQVVVHPREAPQAHEAAQRLKTESVVRAVGAVRPRPEGTVNPDLPTGQVEVAA
                     EAIEVLSEAETPPFPVEDRITADEALRLRHRYLDLRRPQRTRVLRLRHAAAHAIRGYM
                     ERNGFVEVETPMLTRSTPEGARDFLVPSRLQPGSFYALPQSPQLFKQILMVSGLDRYY
                     QIVRCFRDEDLRADRQPEFTQLDVELSFTDEAQVQELVEGLLAEVWEEVLGVTIATPF
                     PRLTYDESMRRFGTDKPDLRYGMELADLTAAFRGSGFNAFRTVVEEGGVVKGLAAPGA
                     AGWSRKDLDQLVVEAQARGAGGLVWVALAGDGVKSPVAKYLSEDELAGAREATGAGEG
                     DLILLVADAEARAGTVLDGMRRLMANRLRLIPEGEWAFLWITEPPLFEWSEEEGRWVS
                     VHHPFTSPVADDLEPASAKARAYDVVLNGWELGGGSIRIHRPEIQRKVFDALGMSREE
                     AEERFGFLLEAFRYGVPPHGGIAFGLDRMVMLLAGMENIRDVIAFPKTQSGADLLTGA
                     PAPVSETQLRELGLRLRGRPEAR"
     gene            complement(2843..4102)
                     /gene="hisS"
                     /locus_tag="VF058_12075"
     CDS             complement(2843..4102)
                     /gene="hisS"
                     /locus_tag="VF058_12075"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019909088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX6209079.1"
                     /translation="MSERFAPPRGTQDLLPPTSDRLEVLASAAARLARASGYRRVETP
                     TFEHTELFARGVGETTDIVTKEMYTFQDKGGRSLTLRPEGTTGVVRAYLGNRQALPTP
                     FKGYYLAPMFRHERPQAGRMRQHTQFGIEVIGTSAPDADAEVIAVGHRFLSRWGLSFE
                     LRVNSIGDPICRPAYREALLAFLRERRDRLSPDCRERMEENPLRVLDCKVPSCREATA
                     DAPIILDFLDDACREHFAVVRDRLDEQGIEHVVDARLVRGLDYYTRTAFEFVSPDLGP
                     TQSTLCGGGRYDGLAEAIGGPATPGIGFGLGLERALLAMERAGLEPPATPGTTVFVVA
                     LGDEAGREGRALARALREEGVSVEHALESRPLGAQLKMADRAGAAYAAILGGDELAAG
                     EVTMRDMETGEQERVKREDVASWLTSG"
     gene            complement(4099..4722)
                     /locus_tag="VF058_12080"
     CDS             complement(4099..4722)
                     /locus_tag="VF058_12080"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6209080.1"
                     /translation="MFLDVRFDNPFQTNCWAVSADGSEEAVVVDPGFQPEQVRDMVER
                     SGRRIAAVLATHGHADHIASVPAVCEPDIPLYIHQADELALTDQGAWGAGMPAPDRYR
                     PQEVRTVKDGDVLEFAGFEIRVRHTPGHTPGSVCYVTDGFLFSGDLVFRDTIGRHDFP
                     NSSSRDMYESLRWFLTLQDDLDVYPGHGPQTTVGRERARNEWLRALV"
     gene            complement(4754..5191)
                     /gene="dtd"
                     /locus_tag="VF058_12085"
     CDS             complement(4754..5191)
                     /gene="dtd"
                     /locus_tag="VF058_12085"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014510273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX6209081.1"
                     /translation="MRIVLQRVARAEVRVGGERVAACGLGLLLLAGVGAEDDGSEIPG
                     LAAKIAGLRVFEDDAGKMNLSLDDVAGEVLLVPNFTLFGDTRKGRRPSWVGAAPPEVA
                     EARVEALCRALEGHGLTVHRGAFGAHMEVELVNDGPVTLVLEA"
     gene            complement(5188..7425)
                     /locus_tag="VF058_12090"
     CDS             complement(5188..7425)
                     /locus_tag="VF058_12090"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX6209082.1"
                     /translation="MEDVRAPERRSGLLARLPFVSSHPDRAEGFDGLLRKVRAFNQKA
                     DIRLIERAYEVAERAHHGQLRKSGEAFIEHPLGVTHVLADLGMDTTTLVAGLLHDVVE
                     DTSVTLEEVEREFGADVAALVDGVTKLDKIPLRTREQHQAENVRKMIVAMARDIRVLI
                     IKLADRLHNMRTLKALRPEKQRQKATETLEIYAPLAHRLGIYSIRWELEDLAFATLHP
                     RRFEEIRNLVGKAEDERESQLEEARASIGKHLKKLKIKAEVTGRPKHLYGVYEKMVLR
                     GKEFSEIFDLLGIRILCETVKDCYGALGAVHAMWKPVPGRFKDYVAMPKFNMYQSLHT
                     VVIGPGGKPLEVQIRTEQMHKTAEFGIAAHWQYKEGKKGKRDETDLAWLRQIMEWQQE
                     TQDPKDFMESLRIDLYEDQIFVFTPKGDVIDLPAGATPIDFAYNIHTEVGHRCMGAKV
                     NGKLVPLDYALRTGDTVEVLTSKAGDAGPSRDWLQVVKSPRARNKIRQWFSRERREDA
                     METGRDLLQRELRRQGLPLKRLTTERVLADLSQDLNYTDLDALLVAIGEGHVSPKSIV
                     VRLERLVTQEDEEEADVPPARPVRIGKQDAGGAVVVQGSSDVWVKLARCCTPVPGDEI
                     MGFVTRGQGVSVHRADCPNAKALENQRDRFIEVSWRTGKPTSFIVSIMVEALDRKKLL
                     GDIATALSEQQVNIISASSGVGKDRVATFRFTFELADIAHLESVLRAIKKVDAVFDAY
                     REVPG"
BASE COUNT         1043 a         2726 c         2577 g         1103 t
ORIGIN      
        1 gggccaggcg cgcgtcgccg ccggccgtct ccgccaggta ggccagcgcc tcgtcctcgg
       61 ccttctcccc ggggagccct cgctcgtcca tcagcgcccg ctccaggatc gtccgcacct
      121 ccccatcggt gaggggctcg agccggagca gcagcgagcg gctcagcagc ggcgagacga
      181 gagagaagta ggggttctcg gtcgtggccc cgatgaaggt gacgtccccc gcctcgacgc
      241 ccgggagcag gacgtcctgc tgggccttcg accagcggtg gacctcgtcc acgaagagca
      301 cggtctgcag catccccccg cgcgccgact ccaggacctt ccgggcgtcg gccaccccgg
      361 aggagaccgc cgacagctgg accagctcgg cccccaccgc cttcgccagg aggtgagcca
      421 gcgtggtctt ccccgtcccc gcgggccccc agagcacgat cgacgggagg tggtcggccc
      481 ccaccagcgc ggccagcgcc ttccccggac cgaggaggtg tcgctgcccc acgacctcgt
      541 cgagggatcg ggggcggatc cgggatgcca ggggctcggt ggccacggcc ccatcataga
      601 gatgcggatg aggcggctcc tactctcgat gggcctgctc tcgcaggtgg gtgccctccc
      661 gcccggttca gcgaacccct ctcggggctc agcacccctg gcggagccgg gctcccctct
      721 tgatggtgtt ggctcaacga gatcaggagc cggatcccat ccgcacccct accttacacc
      781 cgcggggcgg agacccggcg aggatcacgg gcccgccgca ggaacgagcg ccgggctcat
      841 cgtcagcacc tgcacgggcc ccggcgtgag gttccagacg cggccgccgc tgagcacccc
      901 acctcgtccc ggccgccttc gtcgaggtcc accatcgtgg ccaggtgccc gcagccggag
      961 gcggcccgag ggatcgggac gcgccggaag cggccgtcgc cgcggttgag cagcagccgg
     1021 tcggggggcg ttcctgctgc gggccctgga gcctccgatc aagcggatcg gccgggagtg
     1081 agcccgccgg gtcagcgagc ctcgggtcgc ccccggagtc ggaggccgag ctcccgcagc
     1141 tgggtctcgg acacaggggc cggcgccccg gtgagcaggt cggccccgga ctgggtcttg
     1201 gggaaggcga tcacgtcgcg gatgttctcc atccccgcga gcagcatcac catgcggtcc
     1261 aggccgaagg cgatcccccc gtggggcggg acgccgtagc ggaaggcctc caggaggaag
     1321 ccgaaccgct cctcggcctc ctcccgggac atccccaggg cgtcgaagac cttccgctgg
     1381 atctcggggc ggtggatccg gatgctgccg ccccccagct cccagccgtt cagcacgacg
     1441 tcgtaggcgc gggccttcgc cgaggcgggc tccaggtcgt ctgccacggg cgacgtgaac
     1501 gggtggtgca cggacaccca ccgcccctct tcctcgctcc actcgaacag gggcggctcc
     1561 gtgatccaga ggaaggccca ctcgccctcg gggatcaagc ggaggcggtt cgccatgagc
     1621 cggcgcatcc cgtcgagcac ggtgcccgca cgggcctcgg cgtcggccac gagcaggatg
     1681 aggtccccct cccccgcgcc ggtcgcctct cgcgccccgg ccagctcgtc ctcggagagg
     1741 tacttcgcca cgggcgactt gacgccgtcc ccggcgagcg cgacccagac gaggcccccg
     1801 gcgccgcggg cctgggcctc gaccacgagc tgatccaggt ccttccgcga ccagcccgcg
     1861 gcgccggggg ccgcgaggcc cttcacgacg cccccctcct ccaccaccgt gcggaaggca
     1921 ttgaacccgc tcccccggaa cgcggcggtg aggtcggcca gctccatccc gtagcggagg
     1981 tcgggcttgt cggtcccgaa cctccgcatc gactcgtcgt aggtgagcct ggggaacggc
     2041 gtggcgatcg tcacccccag cacctcctcc cacacctccg ccagcagccc ctccacgagc
     2101 tcctggacct gggcctcgtc ggtgaacgac agctccacgt cgagctgggt gaactcgggc
     2161 tggcggtcgg cccggaggtc ctcgtcccgg aagcagcgga cgatctggta gtagcggtcc
     2221 agccccgaga ccatgaggat ctgcttgaag agctggggcg actgcgggag agcgtagaac
     2281 gagcccggct ggagcctgga gggcacgagg aagtcgcggg ccccctcggg cgtcgacctc
     2341 gtgagcatcg gggtctcgac ctccacgaag ccgttccgct ccatgtagcc ccggatcgcg
     2401 tgggccgccg cgtggcgcag ccggaggacg cgggtcctct gggggcgccg gaggtcgagg
     2461 tagcgatggc gcagccggag ggcctcgtcg gcggtgatcc ggtcctcgac ggggaacggg
     2521 ggggtctcgg cctcgctcag cacctcgatc gcctcggcgg ccacctcaac ctggccggtg
     2581 ggcaggtccg ggttgacggt gccctccggg cggggccgca cggcgcccac ggcgcgaacg
     2641 acgctctcgg tcttgaggcg ctgggccgcc tcgtgggcct gcggcgcctc ccgcggatgg
     2701 accaccacct ggatctcccc ctcgcgatcg cggaggtcca cgaagatcac cccgccgtgg
     2761 tcccgggtcg tcgagaccca gccgcagagg gccacccgct cgccggcgtg ctccaggccg
     2821 aggccgccgc acatgtgcgt cctcatccgg aggtcagcca cgaggccaca tcctccctct
     2881 tcacccgctc ctgctccccg gtctccatgt cgcgcatcgt gacctcaccg gcggccagct
     2941 cgtcgccgcc caggatcgcc gcgtaggccg cgccggcccg gtcggccatc ttcagctggg
     3001 ccccgagcgg gcgggactcg agcgcgtgct ccaccgagac gccctcctcc cgaagcgccc
     3061 gggccagggc gcggccctcg cgcccggcct cgtcgccgag cgcgacgacg aagaccgtgg
     3121 tgccgggcgt cgccggaggc tccagtcccg cacgctccat cgccagcagt gcccgctcca
     3181 gcccgaggcc gaagccgatc cccggcgtgg ccggcccccc gatcgcctcg gccagcccgt
     3241 cgtaccgccc tcccccgcac agcgtggact gggtcggtcc aaggtccggg ctcacgaact
     3301 cgaacgcggt gcgcgtgtag tagtcgagcc cgcgcacgag ccgagcgtcc acgacgtgct
     3361 cgatcccctg ctcgtcgagc cggtcccgca ccaccgcgaa gtgctcccgg caggcgtcat
     3421 cgaggaaatc gaggatgatc ggcgcgtcgg ccgtcgcctc tcggcacgag ggcaccttgc
     3481 agtcgagcac ccggagcggg ttctcctcca tccgctcgcg gcagtcgggg gagaggcgat
     3541 cgcgccgctc ccggaggaag gccagcagcg cctcgcggta ggcgggacgg cagatcggat
     3601 ccccgatcga gttcacccgc agctcgaacg acagtcccca ccgggagagg aagcggtgtc
     3661 cgaccgcgat cacctcggcg tcggcgtccg gggccgacgt tccgatgacc tcgatcccga
     3721 actgcgtgtg ctggcgcatg cggccggcct ggggtcgctc gtgccggaac atcggcgcga
     3781 ggtagtagcc cttgaaggga gtgggcagcg cctgccggtt ccccaggtac gcgcggacga
     3841 cgccggtggt gccctcgggg cgaagggtca gcgagcggcc gcccttgtcc tggaacgtgt
     3901 acatctcctt ggtgacgatg tcggtggtct cgcccacacc tcgggcgaac agctcggtgt
     3961 gctcgaaggt gggggtctcg acgcggcggt accctgacgc acgcgccagg cgggctgccg
     4021 cggaggccag cacctcgagg cggtcggagg tcgggggcag caggtcctgg gtgccgcgcg
     4081 gaggggcgaa ccgctcgctc acaccagcgc cctgagccac tcgttccggg cccgctcccg
     4141 ccccaccgtg gtctgaggcc cgtgccccgg gtagacgtcc aggtcgtcct ggagcgtgag
     4201 gaaccaacgc aggctctcgt acatatcccg ggagctcgag ttggggaagt cgtgccgccc
     4261 gatcgtgtcg cggaacacga ggtcacccga gaagaggaac ccgtcggtca cgtagcagac
     4321 gctgcccggc gtgtgcccgg gtgtgtgacg gacgcggatc tcgaagcccg cgaactccag
     4381 gacgtcgccg tccttcaccg tgcggacctc ctggggccgg taccggtccg gcgccggcat
     4441 cccagcgccc caggcgccct ggtcggtcag cgcgagctcg tcggcctggt ggatgtagag
     4501 ggggatgtcg ggctcgcaga cggcgggcac cgaggcgatg tggtcggcgt gcccgtgcgt
     4561 ggccaggacc gcagcgatgc ggcgtccgga acgctccacc atgtcgcgga cctgctcggg
     4621 ctggaagccg gggtccacaa ccactgcctc ttcggagccg tcggccgaga ccgcccagca
     4681 gttcgtctgg aaggggttgt cgaagcgcac gtcgaggaac atccggctca tcgtagtgga
     4741 tacgcggggg cgctcaggcc tccaggacga gcgtgacggg cccgtcgttg accagctcga
     4801 cctccatgtg cgcgccgaac gcgccgcgat gcacggtgag gccgtggccc tcgagggcac
     4861 ggcagagggc ctccacccgc gcctccgcca cctcgggggg cgcggccccg acccaggacg
     4921 gtcgccggcc cttccgcgtg tcgccgaaga gcgtgaagtt cgggacgagg agcacctcgc
     4981 cggccacatc gtccagcgat aggttcatct tgcccgcgtc gtcctcgaac acgcgcaggc
     5041 cggcgatctt cgcggcgagg ccggggatct cggagccgtc gtcctcggct cccacgcccg
     5101 ccagcagcag cagcccgaga ccgcaggcgg cgacccgctc cccgccgacc cggacctcgg
     5161 cccgagccac ccgctggagg acgatcctca gccggggacc tcgcggtagg cgtcgaagac
     5221 cgcgtccacc ttcttgatcg cgcggaggac gctctccagg tgagcgatgt cggccagctc
     5281 gaacgtgaac cggaacgtcg ccacacggtc cttccccacg cccgacgagg ccgagatgat
     5341 gttgacctgc tgctcggaga gcgcggtggc gatgtctccg agcagcttct tgcggtcgag
     5401 cgcctcgacc atgatcgaca cgatgaaaga ggtcggcttc ccggtccgcc acgagacctc
     5461 gatgaaccgg tcacgctgat tctccagggc cttggcgttg gggcagtcgg cccgatgcac
     5521 cgacacgccc tggccccggg tgacgaaccc catgatctcg tcgccgggga cgggcgtgca
     5581 gcaccgtgcc agcttcaccc acacgtcgga ggaaccctgg accacgaccg ccccgcccgc
     5641 gtcttgcttc ccgatccgca cggggcgggc cgggggcacg tcggcctcct cctcgtcctc
     5701 ctgcgtgacg agccgctcca gccgcaccac gatcgacttg ggcgagacgt gcccctcgcc
     5761 gatcgccacc aggagggcgt cgaggtccgt gtagttcagg tcctgggaca ggtcggccag
     5821 aacgcgctcg gtcgtcagcc gcttcagcgg gagcccctgg cgccgcagct cgcgctggag
     5881 caggtcgcgc ccggtctcca tcgcgtcctc acggcgctcc cgcgagaacc actgccggat
     5941 cttgttgcgg gcccggggcg acttcaccac ctggagccag tcgcgcgagg ggccggcgtc
     6001 gccggccttc gaggtgagca cctccaccgt gtcgcccgtc cgcagggcat agtcgagcgg
     6061 cacgagcttg ccgttcacct tcgcccccat gcagcggtga ccgacctcgg tgtggatgtt
     6121 gtaggcgaag tcgatgggtg tggcgccggc cggcaggtcg atcacgtccc ccttcggggt
     6181 gaagacgaag atctgatcct cgtagaggtc gatccgcagg gactccatga agtccttcgg
     6241 gtcctgcgtc tcctgctgcc actccatgat ctggcgcagc caggccagat cggtctcgtc
     6301 gcgcttcccc ttcttcccct ccttgtactg ccagtgcgcg gcgatcccga actcggcggt
     6361 cttgtgcatc tgctcggtgc ggatctgcac ctcgagcggc ttgccccccg gcccgatcac
     6421 gaccgtgtgc agggactggt acatgttgaa cttgggcatc gcgacgtagt ccttgaaacg
     6481 cccgggcacg ggcttccaca tcgcgtgcac cgcgcccaag gccccgtagc agtccttgac
     6541 cgtctcgcag aggatgcgga tcccgaggag gtcgaagatc tcgctgaact ccttgccccg
     6601 caggaccatc ttctcgtaga ccccgtagag gtgcttgggg cggccggtga cctcggcctt
     6661 gatcttcagc ttcttgaggt gtttcccgat cgaggcccga gcctcctcga gctgggattc
     6721 gcgctcgtcc tcggccttcc ccacgaggtt gcggatctcc tcgaagcggc gggggtggag
     6781 ggtggcgaac gccaggtcct cgagctccca tcggatcgag tagatgccga gacggtgcgc
     6841 gagcggcgcg tagatctcca gggtctcggt ggccttctgt cgctgcttct cggggcgtag
     6901 ggccttcagc gtgcgcatgt tgtggagacg gtcagccagc ttgatgatca gcacccggat
     6961 gtcgcgggcc atggccacga tcatcttccg gacgttctcg gcctggtgct gctcgcgggt
     7021 gcggaggggg atcttgtcca gcttcgtgac gccgtcgacg agcgcggcta cgtcggcccc
     7081 gaactcccgc tccacctcct cgagggtgac gctcgtatcc tcgaccacgt cgtggaggag
     7141 cccggccacc agcgtggtcg tgtccatccc cagatccgcc aggacgtggg tcacgccgag
     7201 ggggtgttcg atgaaggcct cgccggactt ccggagctgg ccgtggtggg cgcgctcggc
     7261 cacctcgtag gcgcgctcga tcagccggat gtcggccttc tggttgaacg cccgcacctt
     7321 cctgaggagc ccgtcgaacc cctcggctcg atcgggatgg ctcgacacga agggaaggcg
     7381 cgccaggagg ccgctccgtc gctccggtgc gcgaacgtcc tccacgagtg cacccctctc
     7441 ctgacctcc
//
LOCUS       DASUTW010000356         3009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1291056, whole genome shotgun sequence.
ACCESSION   DASUTW010000356 DASUTW010000000
VERSION     DASUTW010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291056"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VF058_12095"
     CDS             <1..366
                     /locus_tag="VF058_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6209083.1"
                     /translation="LEVVGEATLDTYEEVGGIRSAVIDSEATMPFDFELDVAALAAQL
                     GEEIPEDEAQGTIAYEGEMTMSSRQWIDPETGLQVRLEGGGEGDISFSLGGFPQNGDV
                     PTVPRTNVEFDLTMTLERL"
     gene            363..1271
                     /locus_tag="VF058_12100"
     CDS             363..1271
                     /locus_tag="VF058_12100"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6209084.1"
                     /translation="MSIRRGRDAGLVLLLTPLIWGLTFPAGKEALETLPLLPFTAWSR
                     LLGVATVAIALPFLLRRSRVEARRLVVPGVVLGGLMFVAFLLQSAGLERTTATNAGFV
                     TGLYVVFTPVLGLMLFRRRVTGAAWAAVGISVVGLALLSVPGLDDLSPRPGDLLVLAS
                     AVVWAGHVVAVGHFAVRHPALPLALAQLVGAAGLHLIATAGEGLHVADAIEVWPLLVI
                     TGVLGSGVAFTIQVLAQGTVSATRAAIILAGESVVAAIAAAIWLGERLEAHQWVGAAL
                     AVAAMILSEVGARRRPEVRLDPATAV"
     gene            complement(1301..2905)
                     /locus_tag="VF058_12105"
     CDS             complement(1301..2905)
                     /locus_tag="VF058_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6209085.1"
                     /translation="MPSVRRADVVVVGGGHNGLVCAAYLAKAGKSVVVLERRGVLGGA
                     AVTEEPWPGFKVSTLSYVVSLMPARIIEDLELRKHGFEVYPIDPAYFSPFPDGRGVLI
                     WEDPAKAAKEIAKLNERDAEGYLRYEHDLEELASIVRPLLLRVPPSIELGSWADVREA
                     IGLGRYFFRRRRNLAKLADLMTLSVADFLDQYFVDEAVKGALSPGGVIGAWGGPMTPG
                     SAYVLLHHRMGDAAGPHGAWAFVRGGMGALSGAIARAAAAAGAEIRTGAPVKRIAVAG
                     ERARGVELEDGTTIAARSVVSAAHPHLTFFDLVGREHLPAGIVEDLERYRTRGASAKV
                     NLALSELPDFTAMPGTELGPQHPEFIICPSIPYLERAWDDAKYGRWSAEPMLDCVIPS
                     TKDPTLAPEGKHVLTAFVQYAPYELEEGKTWDDEREAFGDRVVETIAAYAPNVPDAIL
                     HRQVITPRDLEREYGLVGGNIFHGEMSLDQLFSLRPTPMMARHRSPIRGLYLAGSGTH
                     PGGGVMGAPGFNAAKVVRRDLRLRRR"
BASE COUNT          393 a         1098 c         1075 g          443 t
ORIGIN      
        1 ctggaggtcg tcggcgaggc cacgctcgac acgtacgagg aggtgggagg catccggtca
       61 gcggtgatcg acagcgaggc cacgatgccc ttcgacttcg agctcgacgt ggccgcgctg
      121 gcggcccagc tgggcgagga gatccccgag gacgaggccc aggggacgat cgcctacgag
      181 ggcgagatga cgatgagctc tcgtcagtgg atcgaccccg agaccggcct ccaggtccgc
      241 ctggagggag ggggagaggg cgacatctcc ttctcgctcg gcggcttccc ccagaacggc
      301 gatgtcccca ccgtccccag gacgaacgta gagttcgacc tcacgatgac gctcgagcgt
      361 ctgtgagcat ccggcgcgga cgggacgcgg ggctcgtcct cctgctaacg cccctgatct
      421 gggggctgac gttcccggcg gggaaggaag cgctcgagac gctgcccctg ctcccgttca
      481 cggcgtggag ccgccttctc ggggtggcca cggtcgcgat cgcgctgccg ttcctcctgc
      541 ggagatcgag ggtcgaggcc cgccgactgg tggtcccggg cgtcgtgctc ggaggattga
      601 tgttcgtcgc gttcctcctc cagtcggcgg gcctcgagcg gaccaccgcc acgaacgcgg
      661 gcttcgtcac ggggctctac gtcgtgttca cgccggtgct ggggctcatg ctgttccgtc
      721 ggcgcgtgac cggtgcggcc tgggccgcgg tggggatctc cgtcgtgggg ctagcgctgc
      781 tgtcggtgcc cgggctcgac gatctctcgc cgcgccccgg ggatctgctg gtgctggcct
      841 ccgcggtcgt ctgggcgggg cacgtggtgg cggtggggca cttcgcggtg cggcaccccg
      901 cgctaccgct ggcgctggcc cagctggtgg gggcggccgg gctgcacctg atcgcgacgg
      961 cgggggaggg gcttcacgtg gccgacgcca tcgaggtctg gcccctgctc gtcatcaccg
     1021 gggtgctggg gtcgggcgtt gcgttcacga tccaggtgct ggcgcaggga accgtgagcg
     1081 cgacccgcgc cgccatcatc ctggcggggg agtcggtggt ggcggcgatc gcggcggcga
     1141 tctggctggg ggagcgtctg gaggcccacc agtgggtcgg cgccgcgctc gcggtcgcgg
     1201 ccatgatcct gtccgaggtc ggagcgcggc gccgacccga ggtcaggctc gaccccgcca
     1261 cggccgtctg agggccgcgg cgcccggtgg tcggcgaccg tcagcggcgg cggaggcgga
     1321 ggtcccgacg gaccaccttc gccgcgttga agcccggggc gcccatcacc ccgccccccg
     1381 gatgcgtccc cgacccggcc aggtacagcc cccggatggg gctgcggtgg cgcgccatca
     1441 tcggggtcgg ccgcagcgag aacagctgat cgagcgacat ctcgccgtgg aagatgttgc
     1501 cgcccacgag gccgtactcc cgctccaggt cgcgcggcgt gatgacctgg cggtggagga
     1561 tcgcgtccgg gacgttgggc gcgtaggccg cgatcgtctc caccactcgg tcgccgaacg
     1621 cctcgcgctc gtcgtcccag gtcttgccct cctcgagctc gtacggcgcg tactgcacga
     1681 acgccgtcag cacgtgcttc ccctccgggg ccagcgtggg gtccttcgtc gaggggatca
     1741 cgcagtcgag catcggctcg gcgctccacc gcccgtattt cgcgtcgtcc cacgcccgct
     1801 ccaggtaggg gatcgagggg cagatgatga actcggggtg ctgggggccg agctcggtgc
     1861 cgggcatcgc ggtgaagtcg gggagctcgg acaacgcgag gttcaccttg gccgacgctc
     1921 cgcgtgtccg gtagcgctcg aggtcctcga cgatccccgc cggcaggtgc tcgcggccta
     1981 ccagatcgaa gaaggtcaga tgcggatggg cggccgacac caccgaccga gccgcgatcg
     2041 tcgtcccgtc ctcgagctcc acgccgcggg ctcgctcccc ggccacggcg atccgcttca
     2101 ccggcgctcc ggttcggatc tccgcgccgg ccgcagccgc ggctctcgcg atcgccccgg
     2161 acagcgcccc catccctccg cgcacgaacg cccaggcccc gtgcgggccc gcggcgtcgc
     2221 ccatccgatg gtggagcagc acgtaggccg accccggcgt catcggcccg ccccaggccc
     2281 cgatcacccc gccaggggag agcgccccct tcaccgcctc gtccacgaag tactggtcga
     2341 ggaagtcggc caccgagagc gtcatgaggt cggccagctt cgccaggttc ctcctccgcc
     2401 ggaagaagta ccgccccagc ccgatcgcct cgcgcacgtc ggcccacgac cccagctcga
     2461 tcgagggcgg cacccgcagc agcagcggcc gcacgatcga ggccagctcc tcgaggtcgt
     2521 gctcgtaccg caggtagccc tcggcgtccc gctcgttgag cttcgcgatc tccttcgcgg
     2581 ccttcgcggg gtcctcccag atcagcacgc cgcgcccgtc cgggaagggg gagaagtacg
     2641 cgggatcgat cgggtacacc tcgaacccgt gcttgcgcag ctcgaggtcc tcgatgatcc
     2701 gcgcgggcat caggctgacg acgtaggaga gcgtggagac cttgaagccc ggccagggct
     2761 cctcggtgac cgccgcgccg cccagcaccc cgcgccgctc cagcacgacc acggacttgc
     2821 cggccttggc caggtacgcg gcgcacacga gcccgttgtg cccgcccccg accaccacca
     2881 cgtccgcccg tcgcacggag ggcatcctag ccgtccgccg atcgctccgc tcgccgtccc
     2941 aggctagact ccgcgcatgg ccgacctccc cgaagtgacc ctccgggtcc ggatgtccca
     3001 gagcgacgc
//
LOCUS       DASUTW010000357         3160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_774410, whole genome shotgun sequence.
ACCESSION   DASUTW010000357 DASUTW010000000
VERSION     DASUTW010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774410"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..959)
                     /locus_tag="VF058_12110"
     CDS             complement(150..959)
                     /locus_tag="VF058_12110"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEX6209086.1"
                     /translation="MTHHRRDEAAALLAGGGVVALTGAGISQESGIPTFRDPGGIWER
                     FDPDVYGTWEGVMRLAMERPDDLASFLAELRAAFAGAEPGPAHVALARLEAAGLLDAV
                     ITQNVDGLHQRAGSRHVVEVHGSFSRTLCLVCGNRETVSSTEFLVALDRVILGLRTAF
                     IASMASLLPACSRCGGPARPDFVAFGEVPQDMAQAEELARGARVLLVVGTHGEVHPAG
                     ALPEEAAGTGATVIEVSRGHTEIRADIRLRGEAGRVLPPLVEAALTRAGRA"
     gene            complement(956..1648)
                     /locus_tag="VF058_12115"
     CDS             complement(956..1648)
                     /locus_tag="VF058_12115"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX6209087.1"
                     /translation="MTERPRPTWSPLEAFPVGIGVFAGTAVATFLLDVLFDPRTTFVL
                     IGLAFEALLGGLTLLWIRIRGASPAALGLAGGRVARRLVVGFASGLLLFALTVFVVAP
                     ALYAILSLLAGETVIPPQQEVLPEGPSTFHVVLGGLVVVLAAPIGEELFFRGLLFGGL
                     RASMPFWAAALLSAGIFAVVHVYLLLMPLLFLVGFGLAYIYERSGSLLSSIAAHAAFN
                     LIGYLLIVGRFA"
     gene            complement(1645..>3160)
                     /locus_tag="VF058_12120"
     CDS             complement(1645..>3160)
                     /locus_tag="VF058_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6209088.1"
                     /translation="DPEAAAARTLLTGGAERDLLDVVREEGLTAARIGDLAGGLPEAD
                     LVVVGPGPVDLAGVVEAAGQAEILVVVAFPIPSPAMRERGDVVTPIVVARGPEILAEG
                     GAVSGLTSDTTRRAGLVANVDVAPTLLAHLGVPVPGEMTGSPIELRGDPPGSLHERFL
                     ENLALRLPLQLLALAVALAALVAVSLFLGLRPAAPELARALGGWALIVISLPIALLPV
                     SVLPRITWVTALALAALLTAAIVGGAWIVGGRDPTAAVALVGAAGVAALLLDSVAGWP
                     AMMAPIIGGGAFDGVRFHGLGNVYAGVLLAGAVLVAAHLRPWTGVALLSLTALFAGLP
                     GLGSNLGAAATLAAAAGLWGVLRLRRRLGALEILIAAGAAIAGTAIVILAHRLLAPDV
                     SHIARAASDPGGLGALVEDGLRRLGSNLELTSRVPAAWLVIVALVGAAWASARRLGHL
                     ARDPEWRDACLVLALAALVGFVVNDTGIGLAGLAFVYLAAALAYPALEGSWTRR"
BASE COUNT          508 a         1182 c         1178 g          292 t
ORIGIN      
        1 cgagaggaag gcgctcgcga ggagcgccac gagaagcgcc cgcggtgcgc tgagcctccg
       61 agtcataggg tttgtatcgg cctgggcgta ggccgacctg aacccgcacc ctacacgact
      121 cggtctcccg aatcacgcac ctcggctgct caggccctgc cggcccgggt cagcgcggcc
      181 tcgacgaggg gaggaagcac ccgcccggcc tcgccgcgga gacggatgtc cgcccggatc
      241 tcggtgtgac ctcgggagac ctcgatcacc gtggcccccg tccccgcggc ctcctcgggg
      301 agggctcccg ccggatggac ctcaccgtgc gtccccacga ccaggaggac cctggccccg
      361 cgggccagct cctcggcctg ggccatgtcc tgggggacct cgccgaacgc gacgaagtcc
      421 ggtcgggcgg gcccgccgca tcgggagcac gccggaagga gcgaggccat gctggcgatg
      481 aaggcggtcc ggagccccag gatgacccgg tccagcgcca cgaggaactc ggtcgaggag
      541 acggtctcgc ggttcccgca cacgaggcac agcgtccgcg agaaggaccc gtgcacctcg
      601 accacgtgcc ggctccccgc ccgctggtgg aggccgtcca cgttctgggt gatcacggcg
      661 tcgagcaggc cggcggcctc caagcgggcc agggccacgt gcgccggtcc cggctcggcg
      721 cccgcgaacg cggcccggag ctcggccagg aaggaggcga gatcgtcggg ccgctccatg
      781 gccaggcgca tcacgccctc ccaggtcccg tacacgtcgg gatcgaagcg ctcccagatc
      841 ccgccgggat cgcggaacgt ggggatgccc gactcctgcg agatccccgc gccggtgagc
      901 gccacgacgc ccccgcccgc gaggagcgcc gccgcctcgt cccgccgatg atgggtcatg
      961 cgaaccgccc cacgatcagg aggtacccga tcaggttgaa cgccgcgtgg gccgcgatgc
     1021 tggacagcag cgagccgctg cgctcgtaga tgtaggcgag gccgaagccc acgaggaaga
     1081 gcaggggcat gagaagcagg tagacgtgga cgacggcgaa gatccccgcc gagaggagcg
     1141 ccgccgccca gaagggcatc gaggcgcgga ggccgccgaa cagcagtccc cggaagaaga
     1201 gctcctcccc gatcggggcc gccagcacga ccaccagccc gcccaggacg acgtggaagg
     1261 tggaggggcc ctccgggagc acctcctgct ggggagggat gaccgtctcg ccggccagga
     1321 gcgacaggat cgcgtagagg gcaggcgcca ccacgaagac cgtcagcgcg aagaggagga
     1381 ggcccgaggc gaaccccacg acgagccggc gggcgaccct gccccccgcg aggcccaggg
     1441 ccgccgggga cgccccccgg atgcggatcc agaggagggt gaggcccccc agcagggcct
     1501 cgaacgccag gccgatcagc acgaacgtcg tccgcgggtc gaacagcacg tccaggagga
     1561 aggtcgccac ggcggtcccc gcgaagacgc cgatgccgac ggggaaggcc tcgagcgggc
     1621 tccaggtggg ccgggggcgc tcggtcatct cctcgtccac gatccctcca gcgcagggta
     1681 ggccagggcg gccgcgaggt agacgaacgc cagcccggcc aggccgatcc cggtgtcgtt
     1741 caccacgaaa cccaccaggg ccgcgagggc gaggacgagg caggcgtcgc gccactcggg
     1801 gtcgcgggcc agatgcccga ggcgccgggc cgaggcccac gccgccccga cgagcgcgac
     1861 gatcacgagc caggcggcgg ggaccctcga ggtcagctcc aggttgctcc ccagccgccg
     1921 caggccgtcc tcgaccaggg ccccgaggcc cccggggtcg gaggcggccc gggcgatgtg
     1981 ggagacgtcg ggggccaaca gccggtgagc caggatcacg atcgccgtcc cggcgatcgc
     2041 agccccggcg gcgatcagga tctcgagcgc gcccaggcgc cggcggagcc ggaggacccc
     2101 ccagagcccc gccgcagcgg cgagcgtggc ggcggccccc aggttcgacc ccagcccggg
     2161 cagccccgcg aagagcgccg tcaggctcag cagcgccacc cccgtccagg gcctgaggtg
     2221 ggccgcgacg agcaccgccc ccgccaggag gacccccgcg tagacgttgc cgagcccgtg
     2281 gaagcggacc ccgtcgaacg cgccgccgcc gatgatcggg gccatcatcg ccggccagcc
     2341 ggcgaccgag tccaggagga gggcggccac gccggccgcg cccacgagcg ccaccgccgc
     2401 ggtgggatcg cggccgccca cgatccacgc accgcccacg atcgcggccg tcagcagggc
     2461 agccagcgcc agcgccgtca cccaggtgat cctggggagg acgctcaccg ggaggagcgc
     2521 gatcggcagc gagatcacga tcagcgccca tccgccgagg gcccgcgcca gctcgggtgc
     2581 ggcggggcgg agcccgagga agagcgagac ggcgacgagc gcggcaagag cgacggccag
     2641 cgcgaggagc tgcaggggaa gccggagggc caggttctcc aggaaccgct cgtggaggct
     2701 ccccggcggg tcgcccctga gctcgatcgg cgaccctgtc atctccccgg ggaccggaac
     2761 cccgaggtgc gcgagcagag tgggggccac gtccacgttc gccacgaggc cggcccgacg
     2821 cgtggtgtcc gaggtgagcc ccgagacggc gccgccctcg gcgaggatct cggggccccg
     2881 ggccacgacg atgggcgtga ccacgtctcc ccgctcacgc atcgccgggg aggggatggg
     2941 gaaggcgacg accacgagga tctccgcctg gccggcggcc tccacgaccc cggcgagatc
     3001 gaccggcccc gggcccacga ccacgaggtc cgcctcgggg aggccgccgg ccaggtcccc
     3061 gattctcgcc gcggtcagcc cctcttcgcg caccacgtcc agcaggtcgc gctcggcgcc
     3121 tccggtcagg agcgtgcggg cggcggcggc ctccgggtcc
//
LOCUS       DASUTW010000358         9771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1034002, whole genome shotgun sequence.
ACCESSION   DASUTW010000358 DASUTW010000000
VERSION     DASUTW010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9771
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034002"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VF058_12125"
     CDS             complement(<1..592)
                     /locus_tag="VF058_12125"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6209089.1"
                     /translation="MTVNVEDLREHVAEANRRLAEAGVVSLSFGNASGVDRDRGVMAI
                     KASGVACERVQAQDTVVVSLDDGAVVEGTLRPSSDTPTHLVLYRRFDRVGGVVHTHSP
                     FATAWAQAGREIPPFGTTHADHWSGPVPVTPPLTAEEIAGDYEAETGQVIARIFDELG
                     RDPLHLPGILVASHGPFTWGADAAEAATNAMALEILA"
     gene            806..1891
                     /locus_tag="VF058_12130"
     CDS             806..1891
                     /locus_tag="VF058_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX6209090.1"
                     /translation="MLTKWSRPTPARLGLRLVAVLAVLALALAACTDDDGNGNGDDGD
                     QAQGATPAQGGSAGQFCEGMNIVFFPGGTEGGGFETVVYNGARAAEAAFGPSVQYVWS
                     DWDPNNMVTQFQEAMATGPDGIAIMGHPGDDAFGPLVDEARSQGILVTVMNTELQDIQ
                     AQYMSEGTGYVGAVLHDAGYALAQEAISRGGLESGAKVFVWGLVSQPGRGERTQGIID
                     GLEDAGMEVEYQEIDDATNADPAAGVSTFTGYMSANPDTAAIFIDHGNLTSTIPTYLE
                     AANLGPDDLYAAGFDLSPATVQGVEDGFVDLVIDQQQYLQGFFGIQQLCLTHMFGFSG
                     LFVNTGGGFVDASNIEQIAPLVEQQIR"
     gene            1992..2822
                     /locus_tag="VF058_12135"
     CDS             1992..2822
                     /locus_tag="VF058_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6209091.1"
                     /translation="MTASEDGRAPIELRRIHKSFGGVHVLKDVSFTLSAGEVLGLVGD
                     NGAGKSTVIKIITGVHQPDSGQILFRGEEVRDLTVDKARALGVETVFQERALAEQLSL
                     WRNIFMGRPISRAFGFLDVGRMRRITADLMGESMGFTSAVLTPDTHVTGLSGGERQGL
                     AIVRALHFEADIIILDEPTMGLSLKETDKLLHFVKGIKAADKSAIFIDHNIFHVYSVA
                     DRIVVLDRGRVAGDFPTSRYSLEEVTEVMREVAATGSFVEPDRGHATDAAAEHEGGAS
                     "
     gene            2819..3832
                     /locus_tag="VF058_12140"
     CDS             2819..3832
                     /locus_tag="VF058_12140"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6209092.1"
                     /translation="MTSTAEDARAAEALRHGWTPRSFATTYASQIGITVAFVVLWAAF
                     IALASGTFLNSRIYVSFAQTTPYFALPALALTMVIVSGDIDLSFPSIYGLSMVGFVAV
                     ERTAGNVELAVLTALAVGAVAGLLNGVIVTVIGIPALVVTIGTQFLYRGLTLALVSGR
                     SYALVEARDSAAFRILDGRLLGIPMQFVWLTVVALVIWLLLFRHRLGQNGHVIGDNSA
                     AAQLMGIPIRSTRTMMFVVVGVASALAAVMQSLQVSNFYPGVGAGYLLPALAAVFVGG
                     TSVFGGRGTVWGTFIGAFMIGAIQAAIVAMGVAAFWTEVIYGGIILAAVSTHAILQRR
                     FER"
     gene            3880..5385
                     /gene="araA"
                     /locus_tag="VF058_12145"
     CDS             3880..5385
                     /gene="araA"
                     /locus_tag="VF058_12145"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEX6209093.1"
                     /translation="MQTDDLEVWFVTGSQSLYGEETLRTVGEQSARIVRGLGEAPDIP
                     VAVTARPVVTTPDAIRRVCLEANADPRCIGVIAWMHTFSPAKMWIAGLTALQKPLLHL
                     HTQHNSSLPWAEIDMDFMNLNQSAHGDREFAYLVTRLRHRRKTVVGHWEDPAVHHRIG
                     VWTRAARGWHEAQHLKVARFGDNMREVAVTEGDKVEAQIRLGVSVNGYGVSDLADGVA
                     QVADADVDVLVKTYEEDYDVVPELRRGGDRHDSLREAARIELALRAFLDEVGAKAFTD
                     TFEDLGRLRQLPGIAVQRLMAEGYGFGAEGDWKTSVLVRVMKVMAEGLDGGGTSFMED
                     YTYNLGAEHPQILGAHMLEVCPSIASARPTCEIHHLSIGAREDPVRLVFTAPPGPGLV
                     VGLMDLGTRLRLVANEVDVVEPEADLPRLPVARAVWEPRPDLATAAEAWLMAGGPHHT
                     CLTQALSPETLDDFAEIAGIELVTIRGDTDARSFRNELRWNQVYYHLAGAI"
     gene            complement(5392..7095)
                     /locus_tag="VF058_12150"
     CDS             complement(5392..7095)
                     /locus_tag="VF058_12150"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014650069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HEX6209094.1"
                     /translation="MTSRYSVGIDFGTESGRAVLVDVASGAELATAVYEYSNGVIDDH
                     LPPPEDDVRLEPDWALQDPEDYLRTFQVAVPAVLSQSGVDPADVVGVGIDFTACTMLP
                     TTEDGMPLCTIPDLRRRPHAWVKLWKHHAAQPEADRINAVAGERGEAWLPRYGGKISS
                     EWFYSKALQILDEDPEIYERSDRLIEAADWVVWQLTGVETRNNCTAGYKAIWSKKDGF
                     PPGEYFAALDPRFERVIEEKMSSQISFLGDRAGRLSEQAAAWTGLRPGTAVAVANVDA
                     HVSVPAATVTGPGTMVAIMGTSICHMVLGEATATVEGMCGVVEDGIVPGLFGFEAGQS
                     GVGDIFAWFVDNAVPPEYHERAAAQGVDVHTVLEREAAALSPGESGLLALDWWNGNRS
                     VLVDVDLTGLLVGATLATKAHEIYRALIEATAFGTRVIIDSFESNGVAVNEIVACGGL
                     PERNKLLMQIYADVTGREFNIAASGQTPALGSAMFGAVAAGSAEGGYDTIVDAAKNMA
                     GLRDERYTPNPANRAVYDELYEDYRALHDYFGRGPNDVMKRLKATRARTRSGASAVVG
                     S"
     gene            complement(7131..8261)
                     /locus_tag="VF058_12155"
     CDS             complement(7131..8261)
                     /locus_tag="VF058_12155"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX6209095.1"
                     /translation="MRDEDGAKDRLSVPDARSGARTPRRQDKSVTMRDIAAATGLSQS
                     TVSRVLNRTPTVVPIAEKTRDLVIETAKQLGYRPNPLARGLRGASTMLLGVIVRDITD
                     PFFAGAIEAVTVEASGRGYNVVLGHAHARADEAIALWGVLEARHCDAIILLGDMRDQP
                     RLIEDLARAQAPVVALWQGAQPSEIASVTVDNRAGIHSALDHLTELGHRRIAFVGGRQ
                     LGDIKEREEAYTEYLARTGEPVRDGYLQHGPNEPDGGVDALGRLLELPEPPTAIVAAT
                     DVLALGVLHGAHEHGLSVPDRLSVTGFDDIAVATFTVPSLTTVRMPVREMVTEAVRMA
                     LGDRDGPPAGPPAVLQPSLVVRSSTGPVPLESPGSPPSEASG"
     gene            8500..9723
                     /locus_tag="VF058_12160"
     CDS             8500..9723
                     /locus_tag="VF058_12160"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012085048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEX6209096.1"
                     /translation="MNGSPARRGVLWRGTVLGSVVLLFVLTIAPWGVAAPTSAPKLAP
                     PSAPSITVEPFGVVDAEAPDHPEEGNAVDLYTLNNGRGMEITVMTYGGIIQSINVPDR
                     RGNLANVALGFDNLDDYVERNPYFGCITGRYANRIANGMFTLDGVDYQLATNNGPNHL
                     HGGIYGFDKRIWEATEIHDGSTVGLRLTYTSPDGEEGYPGTLEVEVTYLLRRNNSILM
                     NYRATTDAPTIVNLTNHTYFNLAGEGNGDILGHLLKINANRYTPVDPTLIPTGALDPV
                     KGTPMDFRKPHTIGERIEDDFEQLDLGLGYDHNYVLNPKGGHGLALAAWARDPLSGRT
                     LRVRTTEPGIQFYSGNFLDGTLIGTSGQAYEQRFGFALETQHFPDSPNKDHFPSVVLR
                     PGEVYDTTTVYAFGR"
BASE COUNT         1497 a         3347 c         3453 g         1474 t
ORIGIN      
        1 cggccaggat ctcgagcgcc atcgcgttcg tcgcggcctc ggccgcgtcg gcgccccagg
       61 tgaacgggcc gtgagaggcc accaggatcc ccggcaggtg caacgggtcg cgcccgagct
      121 cgtcgaagat gcgggcgatg acctgcccgg tctcggcctc gtagtcgccg gcgatctcct
      181 cggccgtcag cggcggcgtc acggggaccg gcccggacca gtggtcggcg tgggtcgtcc
      241 cgaacggggg gatctcccgg ccggcctggg cccaggccgt ggcgaacggg gagtgcgtgt
      301 ggaccacgcc gcccacccgg tcgaaccgcc ggtacaggac caggtgcgtc ggcgtgtccg
      361 aggacgggcg cagggtcccc tccacgacgg ccccgtcgtc cagggagacc accacggtgt
      421 cctgcgcctg gacccgttca caggccacgc cgctcgcctt gatggccatg acgccccggt
      481 cccggtccac cccgctggcg ttgccgaacg acaacgagac gacgccggcc tccgccagcc
      541 ggcggttggc ctcggccacg tgctcgcgca ggtcctcgac gttcacggtc acccgcatca
      601 tcgcgccgga gcggctgggg cgcccgcttc cggccccccc ggagtcgccg accccttcct
      661 tgacgccaag aatacgtttg catacactcg aatgcaagcg ccccatggtc agggcggcac
      721 atagggggag ctggcgaagg ggggttcgtt cgccgggggg acggccggcg cattcctgca
      781 ggggcttcga aaccaaggag gcaccatgct gacgaagtgg agtcgaccga cgcctgcccg
      841 gctcggtctc cgcctcgtcg cggtgttggc ggtactggcg ctggcgctgg ccgcatgcac
      901 cgacgacgac ggcaacggca acggcgacga cggcgaccag gcccagggcg ccacgccggc
      961 ccaggggggg tcggcgggcc agttctgcga ggggatgaac atcgtcttct tccccggcgg
     1021 cacggaaggc ggcgggttcg agaccgtcgt ctacaacggc gctcgggcgg ccgaggccgc
     1081 gttcggaccc agcgtccagt acgtgtggtc ggactgggat cccaacaaca tggtcaccca
     1141 gttccaggag gccatggcca ccggcccgga cgggatcgcc atcatgggcc acccgggtga
     1201 cgacgcgttc ggaccgctcg tcgacgaggc gaggagccag gggatcctcg tgacggtcat
     1261 gaacaccgag ctccaggaca tccaggccca gtacatgtcc gagggcaccg ggtacgtcgg
     1321 ggcggtcctc cacgacgccg ggtacgcgct ggcacaggag gcgatctcgc gcggcggcct
     1381 cgagtcgggc gcgaaggtct tcgtttgggg cctggtgtcc cagccgggac gcggtgagcg
     1441 gacccagggc atcatcgacg gcctcgagga cgcagggatg gaggtcgagt accaggagat
     1501 cgacgacgcg accaacgccg accccgcagc cggggtgtcg accttcaccg gctacatgtc
     1561 ggcgaacccg gacacggcgg cgatcttcat cgaccacggc aacctgacct cgacgatccc
     1621 gacgtatctg gaggcggcca acctggggcc ggacgacctg tacgcggccg ggttcgacct
     1681 gtcgccggcc accgtccagg gagtggagga cgggttcgtg gacctggtca tcgaccagca
     1741 gcagtacctg cagggcttct tcgggatcca gcagctgtgc ctgacgcaca tgttcgggtt
     1801 cagcggcctg ttcgtgaaca ccggcggcgg cttcgtcgac gcctcgaaca tcgagcagat
     1861 cgcgccgttg gtggagcagc agatccggtg acgcccggcg gcgacgccgt gaagaatgag
     1921 gttgcggccg gcgagtcccc gactcgccgg cccgcctcgg cccgtgccag actctcgacc
     1981 gagagccacc gatgacggcc agcgaagacg gtcgcgcgcc catcgagctc cgccgcatcc
     2041 acaagtcgtt cggcggggtt cacgtcctga aggacgtgag cttcacgctg tcggcgggcg
     2101 aggtgttggg cctcgtaggt gacaacgggg ccgggaagtc caccgtgatc aagatcatca
     2161 cgggggtgca ccagccggac agcggacaga tcctgttcag gggagaggaa gtccgcgacc
     2221 tcaccgtgga caaggcgcgc gcgctcggcg tggagaccgt gttccaggag cgggcgctgg
     2281 ccgaacagct ctcgttgtgg cggaacatct tcatgggccg gcccatcagc cgcgcgttcg
     2341 ggttcctgga cgtcgggagg atgcgccgga tcaccgccga cttgatgggt gagtcgatgg
     2401 ggttcacctc ggccgtgttg actccggaca cccatgtgac cgggctctcc gggggggaac
     2461 gccagggctt ggcgatcgtc cgggcccttc acttcgaggc cgacatcatc atcctggacg
     2521 agccgacgat gggactctcg ctcaaagaga ccgacaagct cctgcacttc gtaaagggca
     2581 tcaaggcggc ggacaagtcg gccatcttca tcgaccacaa catcttccac gtgtattcgg
     2641 tggccgaccg catcgtggtc ctggatcgcg gacgggtcgc cggcgacttc ccgacctcgc
     2701 gctattcgct cgaggaggtc accgaggtca tgcgggaggt cgccgcgacc gggagcttcg
     2761 tcgaacccga ccgcggccac gccaccgacg cggcggccga gcacgaaggg ggggcgtcgt
     2821 gacgtcgacg gccgaggacg cccgggcggc cgaagccctc cgccacgggt ggacgccccg
     2881 gtccttcgcc accacgtacg cgtcgcagat cggcatcacc gtcgcgttcg ttgtgctctg
     2941 ggccgcgttc atcgccctcg cgtccggcac attcctgaac agccgcatct acgtgtcgtt
     3001 cgcgcagacc accccatact tcgccctccc cgcactcgcg ctgacgatgg tcatcgtgag
     3061 cggggacatc gacctgtcgt tcccgtcgat ctacggcctc agcatggtcg ggttcgtggc
     3121 cgttgagcga accgccggca acgtcgagct ggccgtcctg accgccctcg cggtcggggc
     3181 cgtcgccggg ctgctgaacg gggtgatcgt caccgtgatc ggcatcccgg cgctggtggt
     3241 cacgatcggg acccaattcc tgtaccgggg gctgacgctg gccctggtga gcggaaggag
     3301 ctacgccctg gtcgaggccc gggactcggc cgcgttccgg atcctggatg ggaggctcct
     3361 cggcatcccg atgcagttcg tctggctcac cgtggtggcg ctcgtgattt ggctgctgct
     3421 gttccggcat cgactcgggc agaacggcca cgtgatcggg gacaacagcg cggccgctca
     3481 gctcatgggc atcccgatcc ggagcacgcg cacgatgatg ttcgtggtcg tcggcgtggc
     3541 gtcggcgctc gccgcggtga tgcagagcct ccaggtctcg aacttctacc ccggggtcgg
     3601 agcggggtac ttgctgccgg cgctggcggc cgtgttcgtc ggtgggacgt cggtgttcgg
     3661 cggccggggg acggtctggg ggacgttcat cggcgccttc atgatcggcg cgatccaggc
     3721 cgcgatcgtc gccatgggcg tggccgcgtt ctggaccgag gtgatctacg gcgggatcat
     3781 cctcgccgcc gtctcgaccc acgccattct tcaaagacgc ttcgagcggt gagcgcgatg
     3841 cgcggggacg cggcctcgcg ctccggagga ggatcgacga tgcaaacgga cgatctcgag
     3901 gtctggttcg tcaccggaag ccagagcctc tacggggagg agacgcttcg cacggtgggg
     3961 gagcagtccg cgcggatcgt gcgcggcctg ggcgaggctc ccgacatccc ggtcgccgtg
     4021 acggctcgtc cggtcgtcac cacgccggac gccatccggc gggtctgcct cgaggcgaac
     4081 gccgaccctc gctgcatcgg cgtgatcgcg tggatgcaca cgttctcgcc ggccaagatg
     4141 tggatcgccg ggctcacggc gctgcagaag ccgctcctgc acctgcacac gcagcacaac
     4201 tcgtcgctgc cgtgggccga gatcgacatg gacttcatga acctgaacca gtcggcccac
     4261 ggcgaccgcg agttcgccta cctcgtgacc cggctccgcc accgtcgaaa gacggtcgtg
     4321 ggccactggg aggacccggc ggtgcaccac cggatcgggg tgtggacccg ggcggcgcgg
     4381 gggtggcacg aggctcagca cctgaaggtg gcgcggttcg gcgacaacat gcgggaggtc
     4441 gcggtcaccg agggcgacaa ggtcgaggcg cagatccggc tcggcgtctc ggtgaacggc
     4501 tacggcgtga gcgacctggc cgacggcgtc gcgcaggtcg cggacgccga cgtggacgtg
     4561 ctggtgaaga cgtacgaaga ggactacgac gtcgtgcccg agctccgccg gggcggcgac
     4621 cgccacgact cgctccgcga ggccgcacgg atcgagctcg cgctgcgggc gttcctcgac
     4681 gaggtcgggg ccaaggcgtt caccgacacg ttcgaggacc tgggcaggct ccgccagctc
     4741 ccgggcatcg ccgtccagcg gctgatggcc gaggggtacg ggttcggcgc cgagggcgac
     4801 tggaagacct cggtgctggt ccgggtgatg aaggtgatgg ccgagggcct ggacggcggc
     4861 ggcacctcgt tcatggagga ctacacctac aacctcggcg ccgaacaccc ccagatcctg
     4921 ggggcccaca tgctcgaggt ctgtccgagc atcgcgtcgg cccggccgac gtgcgagatc
     4981 caccacctgt cgatcggcgc gcgcgaggac cccgtgcggt tggtcttcac cgcgccgccg
     5041 gggcccgggc tcgtggtcgg cctgatggac ctcgggacca ggttgcgcct ggtggccaac
     5101 gaggtggacg tggtcgagcc cgaggcggac ctgccgcgcc tgccggtcgc ccgcgccgtc
     5161 tgggagccgc ggccggacct ggccacggcg gcggaggcct ggctcatggc cggcgggccg
     5221 caccacacct gcctcaccca ggcgctctcg ccggagacgc tcgacgactt cgccgagatc
     5281 gcggggatcg agctcgtgac gatccgtggc gacaccgacg cgcgcagctt ccgcaatgag
     5341 ctgcgctgga accaggtcta ctaccacctg gccggcgcca tctgatcgcg gttacgaccc
     5401 cacaacggcc gaggcacccg agcgcgttcg cgcccgggtc gccttgaggc gcttcatgac
     5461 gtcgttcggc ccgcggccga agtagtcgtg cagggcccgg tagtcctcgt acagctcgtc
     5521 gtagacggcc cggttcgccg ggttcggcgt gtaccgctcg tcgcggagcc ccgccatgtt
     5581 cttcgccgcg tccacgatcg tgtcgtaccc gccctccgcc gatcccgcgg ccaccgcccc
     5641 gaacatcgcc gaaccgagcg ccggggtctg gcccgaggcg gcgatattga actcgcgccc
     5701 ggtgacgtcg gcgtagatct gcatcaggag cttgttgcgc tcgggcagcc cgccgcacgc
     5761 cacgatctcg ttcaccgcga cgccgttgga ctcgaacgag tcgatgatca cgcgggtccc
     5821 gaacgcggtc gcctcgatca gcgcccggta gatctcgtgg gccttggtgg ccagggtcgc
     5881 gcccaccagc agccccgtga gatcgacgtc caccaggacg gaccggttgc cgttccacca
     5941 gtcgagcgcg agcaggcccg actcgccggg ggagagcgcc gcggcctcgc gttcgaggac
     6001 cgtgtgaacg tccacgccct gggcggcggc ccgctcgtgg tactcgggtg ggacggcgtt
     6061 gtccacgaac caggcgaaga tgtcgccgac gccggactgc ccggcctcga acccgaacag
     6121 cccggggacg atgccgtcct cgaccacacc gcacatgccc tcgacggtgg ccgtcgcctc
     6181 cccgaggacc atgtggcaga tgctcgtgcc catgatcgcc accatggtgc ccggtcccgt
     6241 cacggtggcc gcggggacgg agacgtgcgc gtcgacgttc gcgaccgcga ccgcggtccc
     6301 cggccgaagg ccggtccacg cggcggcctg ctcggacagc cggccggccc ggtcgcccag
     6361 gaagctgatc tgggaggaca tcttctcctc gatcacgcgc tcgaaccggg gatcgagggc
     6421 cgcgaagtac tcacccggcg ggaacccgtc cttcttggac cagatggcct tgtaaccggc
     6481 ggtgcagttg ttccgggtct cgacgccggt gagctgccac accacccaat ccgcggcctc
     6541 gatcagtcgg tcggaccgct cgtagatctc cgggtcctcg tcgaggatct ggagcgcctt
     6601 ggagtagaac cactccgagg agatcttgcc gccgtagcgt gggagccacg cctcaccccg
     6661 ttcgccggcg accgcgttga tccggtcggc ctcgggttgg gcggcgtggt gtttccacaa
     6721 cttgacccag gcgtggggcc gccgccggag gtcggggatc gtgcagagcg gcatcccgtc
     6781 ctcggtcgtg gggagcatgg tgcaggcggt gaagtcgatg ccgacgccca cgacgtcggc
     6841 cgggtccacg ccggactggg agagcaccgc cggcacggcg acctggaagg tgcggaggta
     6901 gtcctcgggg tcctggaggg cccagtcggg ctcgagccgg acgtcgtctt ccgggggcgg
     6961 gagatggtcg tcgatgacgc cgttcgagta ctcgtagacg gccgtggcca gctcggcgcc
     7021 gctggcgacg tccaccagga ccgctcgccc ggactcggtc ccgaagtcga tcccgacgct
     7081 gtatctgctc gtcatgtcgc tgccccctcg tgttcggacg gacgaacggt tcagcccgag
     7141 gcctcggacg ggggagaccc cggtgattcc agcggcactg ggccggtcga ggaccgcacg
     7201 accaacgacg gctgcaggac ggcgggcggg cctgcggggg ggccgtcccg gtcgcccagg
     7261 gccatccgaa ccgcctcggt caccatctcg cggacgggca tgcgcacggt cgtgagcgac
     7321 gggaccgtga acgtcgccac ggcgatgtcg tcgaacccgg tgaccgagag acggtcgggg
     7381 accgacaggc cgtgctcgtg ggcgccgtgg agcacgccca gcgccagcac gtcggtcgcg
     7441 gcgacgatgg cggtgggagg ttccgggagc tcgaggagcc gccccagcgc gtcgacgccc
     7501 ccgtcgggtt cgttgggccc gtgctggagg tagccgtcgc ggaccggctc gccagtcctc
     7561 gccaggtact cggtgtaggc ctcctcgcgc tcttttatgt ccccgagctg ccgcccgccg
     7621 acgaacgcga tccggcgatg gccgagctcg gtcaagtgat cgagggccga gtggatcccg
     7681 gctcggttgt cgacggtgac cgatgcgatc tccgagggct gggcgccctg ccagagcgcg
     7741 acgaccggag cctgggctcg ggccagatcc tcgatcaggc ggggctggtc cctcatgtcg
     7801 ccgagcagga tgatcgcatc gcagtggcgg gcctcgagca cgccccacag ggcgatcgcc
     7861 tcgtcggcgc gagcgtgagc gtggcccagg acgacgttgt agccgcggcc gctggcctcg
     7921 accgtcaccg cctcgatggc tccggcgaag aacgggtccg tgatgtcgcg gacgatcacc
     7981 ccgagcagca tcgtgctggc gccgcgcaga ccccgggcca gggggttcgg ccggtagccg
     8041 agctgtttgg cggtctcgat gaccaggtcg cgggtcttct ccgcgatcgg caccaccgtc
     8101 ggggttcggt tcagcacccg ggagacggtg ctctgcgaca ggcccgtggc ggcggcgatg
     8161 tcccgcatcg tcacggactt gtcctggcgc cggggggtcc gtgccccgga ccgggcgtcg
     8221 ggcaccgaca gccggtcctt cgcgccgtct tcgtccctca tggtcctccg catcttatgg
     8281 acaaacgacc ggcagatgtg ataggcagca taccatgtac tgcatacgtt gccacgcccg
     8341 ggcccgaggt cctccgtgta ctcccagtgc ttcggcctga ccggtcggga gacccgggga
     8401 ttgcgtcgac gcgcaacgcg cggcccggct gggatccctg ggaggcggcc ggtcggagcc
     8461 gggtcgtgaa ccggacacgc cgaccggaag gagacgtgaa tgaacggttc gcccgcacga
     8521 cgcggcgtcc tgtggagggg cacggtcctc gggtccgtag tcttgttgtt cgtcctcacg
     8581 atcgccccgt ggggggtggc ggccccgacg tcggcgccga agctcgcacc cccgtcggcg
     8641 ccgtcgatca ccgtggagcc cttcggcgtg gtggacgccg aagcccccga ccatcccgaa
     8701 gaaggcaacg ccgtcgatct ctacaccctc aacaacggta gggggatgga gatcacggtg
     8761 atgacgtacg gcggcatcat ccagtcgatc aacgtgccgg accgccgggg caatctggcg
     8821 aacgtcgctc tcgggttcga caacctggac gactacgtcg agcgcaaccc gtatttcgga
     8881 tgcatcacgg gccggtacgc gaaccggatc gccaacggga tgttcacgct cgacggcgtc
     8941 gactaccagc tcgccacgaa caacggcccc aatcacctcc acggggggat ctacgggttc
     9001 gacaagcgga tctgggaggc caccgagatc cacgacggtt ccaccgtcgg cctgaggctc
     9061 acatacacga gcccggacgg tgaggagggc tacccgggaa cgctcgaggt cgaagtgacc
     9121 tacctgctga ggaggaacaa ctcgatcctg atgaactacc gggcgacgac ggacgccccg
     9181 acgatcgtca acctaaccaa ccacacctat ttcaacctcg ccggtgaagg gaacggcgac
     9241 atcctcggcc acctgctgaa gatcaacgcc aaccggtaca cgccggtcga cccaacgctc
     9301 atcccgactg gcgccctcga tccggtgaag ggcacgccga tggacttccg caagccgcac
     9361 accatcggtg agcgcatcga ggacgatttc gagcagctcg acctcggcct tgggtacgac
     9421 cacaactacg tgctgaaccc caagggcggc cacgggctcg ccttggccgc gtgggcgcgt
     9481 gaccccttga gcggacgtac gctccgggta cgcacgacgg agccggggat ccagttctac
     9541 tcggggaact tcctcgacgg gaccctgatc ggcacgagcg gtcaggcgta cgagcagagg
     9601 ttcggcttcg cgctggagac ccagcatttc cccgactcgc cgaacaagga ccacttcccg
     9661 tcagttgtcc ttcgccccgg ggaggtctac gacacgacga cggtctacgc gttcggtcgc
     9721 tagcgccaga ctcgtcgaga acggcgacgc gggagccggt cctcggatcg g
//
LOCUS       DASUTW010000359         2556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_647428, whole genome shotgun sequence.
ACCESSION   DASUTW010000359 DASUTW010000000
VERSION     DASUTW010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647428"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VF058_12165"
     CDS             complement(<1..207)
                     /locus_tag="VF058_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6209097.1"
                     /translation="MTIFMYATNLTIERITWEQALVRYPAQALLVVAVGMSVPMIPWM
                     RFRGHSTRSSYEMAAAMAVPVIPFV"
     gene            730..1965
                     /locus_tag="VF058_12170"
     CDS             730..1965
                     /locus_tag="VF058_12170"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX6209098.1"
                     /translation="MLIAIRGRRSGAQYTFPVQYAEGGGAIWVFPGHHERKTWWRNLA
                     SESSVTLHLRGHDVEATAEAFSGKGAPSVVEEGLYTYLRRFRAVGRRMGVVSRNGKVD
                     QERLGELAKSRVIVRIEPLAHGIVSPRIEGVHERPEMPGLLGPIRRHPLGAFYALTFL
                     LSWGYWVPNTLAGGKWSHSPGLLGPAISAIVVSGVSQGSGGLRGLADRILRWRVHPRW
                     YLWVAFPVGLALVVAGVLSLGGGEFPSFAEWSEMAGFAGVSGWAAYGVILVVNGFGEE
                     TGWRGFATPAFRRRHNEITSSLLVAIPWALWHLPTFFIDSGYRGFPLFFLPGWLLGVF
                     ALAVVMTWIYEGARSSILIAVLMHLSLNVATTTAATEGTVSAVVTMAVVVSSIIIAAR
                     WRAAAEGAGVHPGRSGYRE"
     gene            1984..>2556
                     /locus_tag="VF058_12175"
     CDS             1984..>2556
                     /locus_tag="VF058_12175"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6209099.1"
                     /translation="MSEDLADPGAGHVAGLGAELHLDHVTVEVPDLPAAVGQFEEQLG
                     LRVSVSPSAPQRHGRVYLARAYLEVAARPDGPTWDVTHVFLRFSDPQQLRSHLEEAEL
                     VYRFQVYEGVDGRWDDVEIDAAGVPMPILVRRTEPAEVARDWPPPLDHPHPGGAFALA
                     AVHIPVASIDAAAPLYSRLLAVEAPRDCTGP"
BASE COUNT          376 a          872 c          856 g          452 t
ORIGIN      
        1 gacgaacgga atcacgggaa cggccatggc cgccgccatc tcgtacgagc tcctcgtact
       61 gtggccccgg aaccgcatcc acgggatcat cggcacactc atgcccacag ccaccacgag
      121 caacgcctga gctgggtatc gaaccagcgc ctgttcccac gtgatgcgct cgatcgtcaa
      181 gttggtcgcg tacatgaaga tggtcacggc cgcgatcatc ccgatcgcca tgaccgccgc
      241 catctcggcg taatgccgag cgaaggaccg ccacgcatgc gtgccaggag cgacccgctc
      301 ggccccgcct gagtcagggt ccgcccggcg cgagtggtct gtccttacgg tgctcatctc
      361 gctcaccccc ttcgccgaaa cggctggccc cgcccatcgg cggggtaccg gctcgatgga
      421 gaggacgctc ctccccgcat gccggcgcag ggagggccgc ctggccccct gttggtgggc
      481 cgtgggccct ctggggcgcc ggccggtcaa gtccggacgg ggccccgtcc gtggccgttc
      541 ccccggttca gagctccccg gcaggccctc gaccagaggg acctacggcc cttttctgga
      601 cctgccccgc agaccagtct cctggcaggt gattcgatga aatctggagg cacccgttct
      661 cccgtcaccc gacgattcat ggcgtggctt cttcgctccc ccttcgctga cctcttggac
      721 gggtcggtca tgctgatcgc aattcgaggg agacgctccg gggcccaata caccttcccg
      781 gttcagtacg cggagggggg cggggccatc tgggtcttcc ccggtcatca cgaacggaag
      841 acctggtggc gcaatctcgc ctcagagtca tcagtgaccc tccatctgcg gggacacgat
      901 gtggaagcga cggccgaggc tttctccggg aagggagcac cgtcggtcgt tgaggaggga
      961 ctctacacct acctgcggcg gttccgtgcg gttggcaggc ggatgggggt ggtgagcagg
     1021 aacggcaagg tcgaccagga acggcttggt gagctggcga agagccgtgt catcgttcgg
     1081 atcgagccat tggcacacgg catcgtgtcg cctcggattg agggcgtcca cgaacggccc
     1141 gagatgccgg gactccttgg acccatccga cgccatccac tcggagcgtt ctacgccctc
     1201 accttcctgc tgtcgtgggg gtactgggtg ccgaacacgc tcgcaggcgg gaagtggagc
     1261 cattccccgg gactcctggg gccggcgatc tcggcgatcg tcgtgagcgg cgtcagccag
     1321 gggtccggcg ggctgcgggg gctcgcggat cgcatccttc ggtggagggt ccacccgcgc
     1381 tggtacctct gggtagcctt ccccgtcggc ctggcactcg tcgtggcggg cgtcctatcg
     1441 ctgggcggcg gtgagttccc ctcgttcgcg gaatggagcg agatggcggg gtttgccggc
     1501 gtcagcgggt gggccgcgta cggggtcata ttggtcgtca acggtttcgg cgaggagacc
     1561 ggctggcgag gattcgccac accggcgttc cgccggaggc acaacgagat cacctcgagc
     1621 ctcctcgtgg cgatcccgtg ggccctgtgg catctgccca ccttcttcat cgacagcgga
     1681 taccggggct tccctctctt cttccttccg ggctggctcc tcggcgtctt cgccctggcc
     1741 gtggtgatga cgtggatcta cgagggcgcc cggtccagca tcctgatcgc cgtgctgatg
     1801 catctttcgt tgaacgtcgc aaccacgacg gctgcgacgg aaggaacggt cagcgccgtg
     1861 gtgacgatgg cggtggtcgt ctcgtcgatc atcatcgcgg cgagatggcg ggcggccgca
     1921 gaaggggccg gagtccaccc cggacgttcc ggctatcggg agtgatgcga cgtgagcggc
     1981 acgatgagcg aggacctcgc ggaccctggc gcaggacacg tcgcgggcct cggtgcggag
     2041 cttcacctcg accacgtgac ggtcgaggtt cccgatctcc ccgccgcggt cggccagttc
     2101 gaggaacagc tcggcttgcg agtgtcggtg agcccgtctg caccccaacg ccacggccgc
     2161 gtgtacctgg ctcgcgccta cctcgaggtc gcggctcgcc cggacggccc aacgtgggac
     2221 gtcacgcacg tcttcctacg cttctccgac ccccagcaac ttcgatccca tctcgaagaa
     2281 gcggaactcg tctatcggtt ccaggtctat gagggcgtcg atgggcggtg ggacgacgtc
     2341 gagatcgacg ccgccggtgt cccgatgccg atcctcgtac gacgcacgga gccagcagag
     2401 gtggctcgcg actggccgcc acccctcgat cacccccatc ccggcggggc cttcgctctc
     2461 gccgcggttc acatcccggt ggcatcgatc gacgcggcgg cccccctcta ctctcgtctg
     2521 ctcgctgtcg aggcgccgcg tgactgcacg ggccca
//
LOCUS       DASUTW010000360         2592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_128572, whole genome shotgun sequence.
ACCESSION   DASUTW010000360 DASUTW010000000
VERSION     DASUTW010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128572"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..165)
                     /locus_tag="VF058_12180"
     tRNA            complement(93..165)
                     /locus_tag="VF058_12180"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(130..132),aa:Ala,seq:ggc)
     gene            235..561
                     /locus_tag="VF058_12185"
     CDS             235..561
                     /locus_tag="VF058_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6209100.1"
                     /translation="MSDETVLARFPSEGGDLVIRPDTLQLGDGQRIRLEDVKGVRTRG
                     RELEIRQADGGGAAIRLAGDEEAADAEQVLNKLLSQASGDRNVTEGLEGSMGGALGDG
                     SPPETP"
     gene            complement(565..933)
                     /gene="rsfS"
                     /locus_tag="VF058_12190"
     CDS             complement(565..933)
                     /gene="rsfS"
                     /locus_tag="VF058_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018161268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX6209101.1"
                     /translation="MTGSKQLAITAARAASDKQATDILVLDVRDLIVITDYFVIASGR
                     SDRQVKTIAESIEEALREEGEKPLRREGRRDGRWILLDYLDFVVHVFLDEERDFYGLE
                     RLWADAPVIDWNHAPARSGG"
     gene            complement(930..2501)
                     /locus_tag="VF058_12195"
     CDS             complement(930..2501)
                     /locus_tag="VF058_12195"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX6209102.1"
                     /translation="MKSLEELIEEIKREARGEPPEEARFEAPVFEAPGPASGPAIEPE
                     AFEAYEEWEPWLLAETEPSEDPEPEPEPGPVEPVPPLEPEPPIVEPRPPLPDEPGEDD
                     EDGGAAGTAPTEADPDEVDFDHLLDLVGGPRAPARVELRPTRPPLEPEILEAQPAETL
                     GLPSSEPLPEPVPETRFRPRVVKGLRYGVLGLGILVFVIGATIWLAGPAPDRDPNPSG
                     VAVGAAADQSVVAWSVWDGETSEGAFVTVVTLGGGKAPVALSIPSYTMANIPGYGTAP
                     VGDAIAAEGGDPGAAAVENLLGIGIDASAFSTLEDLRQTIDELGGIAIAETTMSGEDA
                     VRYLMRGGSDSPISAEFQFLRWQELVAGMLDAVNGRTEVFTSLGPEVGPVLAAAGSAG
                     AEVLELPVVDIGAGLARPDAEEVQRIVEQRFLPTARTTRTVRLSVLNGVGTPGVGERV
                     SKILGPAGFKLVASGNAQTFDFKETQIIATTEEFLPESELARDLLGVGKVYVSAQPSG
                     LVDVTVIVGSDFGGT"
     gene            complement(2498..>2592)
                     /locus_tag="VF058_12200"
     CDS             complement(2498..>2592)
                     /locus_tag="VF058_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinic acid mononucleotide
                     adenylyltransferase"
                     /protein_id="HEX6209103.1"
                     /translation="IRERVAKGRPIRYLVPEGVKSYIEKAGLYR"
BASE COUNT          373 a          937 c          896 g          386 t
ORIGIN      
        1 gtttgctgcc gttctgctgc cttttaaccg accagcctgt tggcgaggga tgcgacgatc
       61 agggaaggcc gcatgaccag gcgttttcgc tgtggacctg aggggagtcg aacccctgac
      121 ctcctgcctg ccgagcaggc gctctaccag ctgagctaca ggccctcgca acgaccgatt
      181 ctaccagcag gtcctagccg cttggccgtg gccggcaacg ggtaacgaca ggtcatgtcc
      241 gacgagacgg tgctggctcg gttcccatcc gagggcggcg acctcgtgat ccgtcccgac
      301 accctccagc tgggtgacgg tcagcggatc cgcctcgagg acgtcaaggg cgtgcgcacc
      361 cgaggccgtg agctggagat ccgtcaggcc gacgggggcg gcgcggcgat ccgcctcgcc
      421 ggcgacgagg aagcagccga cgccgagcag gtcctgaaca agctcctctc gcaagcgagc
      481 ggtgaccgaa acgtcaccga ggggctcgag ggctccatgg ggggcgcgct cggcgacggc
      541 tccccgccgg agacgccctg agcgtcagcc gcccgaccgc gcgggggcgt ggttccagtc
      601 gatgacggga gcgtccgccc acagccgctc cagcccgtag aagtcccgct cctcatcgag
      661 gaacacgtgc accacgaagt cgaggtagtc gaggaggatc caacggccgt cgcggcgacc
      721 ttcgcgccgc agaggcttct ccccctcttc ccgcagggcc tcctcgatcg actcggcgat
      781 cgtcttgacc tgccggtcgc tccgcccgct cgcgatcacg aagtagtcgg tgatgacgat
      841 gaggtcccga acgtcgagga cgaggatgtc ggttgcctgc ttgtcggagg cggcgcgggc
      901 tgctgtgatg gccagctgct tcgatcccgt cacgttcctc cgaaatccga tcccacgatc
      961 acggtcacgt cgaccagccc gctgggctga gcgctgacgt acaccttacc cacgccgagg
     1021 agatcacgcg caagctccga ttcgggcagg aactcctctg tcgtggcgat gatctgcgtc
     1081 tccttgaagt cgaaggtctg agcgttgccc gaggccacga gcttgaagcc cgcgggcccg
     1141 aggatcttcg agacgcgctc gcccacaccg ggggtcccca ccccgttcag gaccgagagg
     1201 cggacggtgc gggtggtccg ggcggtgggg aggaagcgct gctcgacgat ccgctggacc
     1261 tcctcggcgt ccgggcgggc caggcccgcg ccgatgtcca ccaccgggag ctcgagcacc
     1321 tcggcccccg cgctgcccgc cgcggccagc acgggaccga cttcgggccc cagcgaggtg
     1381 aacacctcgg tccgtccgtt caccgcgtcg agcatccccg ccacgagctc ttgccaccgc
     1441 aggaactgga actcggccga gatcgggctg tcgctcccgc ccctcatcag gtaccgcacc
     1501 gcgtcctcac cgctcatcgt ggtctcggcg atcgcgatgc cgccgagctc gtcgatcgtc
     1561 tggcggaggt cctccagcgt cgagaaggca gaggcgtcga tgcctatgcc gaggaggttc
     1621 tccacggccg cggcgccagg gtcgccgccc tccgcggcga tcgcgtctcc gacgggcgcc
     1681 gtgccgtagc cggggatgtt cgccatcgtg tacgagggga tgctgagggc gacgggggcc
     1741 ttcccaccgc ccagagtgac gaccgtgacg aaggcgccct cgctcgtctc cccgtcccag
     1801 accgaccagg ccacgacgct ctggtcggcc gctgcgccga cggccacgcc cgaagggttc
     1861 ggatcgcggt cgggcgcggg acccgcgagc cagatggtgg caccgatcac gaagacgagg
     1921 atccccagcc cgagcacccc gtagcggagg cccttgacca cccggggccg gaaccgggtc
     1981 tcggggaccg gctcggggag gggctccgag gagggcagcc cgagcgtctc cgcaggctgg
     2041 gcctcgagga tctcgggctc gaggggcggt cgcgtgggcc gcagctccac ccgcgcgggg
     2101 gcccgcgggc cgcccaccag gtcgaggagg tggtcgaagt ccacctcgtc gggatccgcc
     2161 tcggtgggag ccgtgcccgc cgctcccccg tcctcgtcgt cttccccggg ctcatcgggg
     2221 agcggcgggc gcggctccac gatcgggggt tcgggctcga gcgggggtac cggctccacg
     2281 ggccccggct cgggctcggg ctcagggtcc tcggaaggct cggtctccgc gagcagccag
     2341 ggctcccact cctcgtacgc ctcgaaggcc tcgggctcga tcgcgggacc cgaagcgggc
     2401 ccgggagcct cgaacacggg cgcctcgaac cgcgcctcct ccggaggctc cccgcgcgcc
     2461 tcgcgcttga tctcctcgat cagctcttcc aggctcttca tcggtacagg cccgccttct
     2521 cgatgtagct cttcacgccc tccggcacga ggtaccggat gggcctgccc ttggcgacgc
     2581 gctcccggat gt
//
LOCUS       DASUTW010000361         6344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_387877, whole genome shotgun sequence.
ACCESSION   DASUTW010000361 DASUTW010000000
VERSION     DASUTW010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6344
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387877"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VF058_12205"
     CDS             complement(<1..790)
                     /locus_tag="VF058_12205"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6209104.1"
                     /translation="MTEAVVEVRGVGKRFGRRDASVEAIRSVDLSLAPTDFVALVGPS
                     GCGKSTLLNIVGGLLHPSRGTVMVKGHPVDGPPPEVGMMFQNPVLLDWRTARDNVLLP
                     IEIRGGRAAVEAARPHADELLELVHLAGFEDKYPSELSGGMQQRVAICRMLIADPEVL
                     LLDEPFGALDELTREHMNVELARIFSAAAKAAIFVTHNIQEAVFLSDRVYVITPRPGR
                     VAGVVDVGLPRPRSLDLVTTEPFQRLARQVRRLLDVGAEGEPSGV"
     gene            complement(809..1870)
                     /locus_tag="VF058_12210"
     CDS             complement(809..1870)
                     /locus_tag="VF058_12210"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6209105.1"
                     /translation="MRRSRWLVALLALMLVAGACGGDDGEEAGGGGDGEEQPQEITEL
                     TALIPFPSGAVFYPLYVAQDRGYFEEEGLSVTTEAVDGSGQILQQVLAGQAEVGLPSP
                     GPFMNSVLEGSDLVSVYTLFQSNVFSLQTLADSQVQSVADLEGTTVGVGTIEGGETPF
                     VRAVLSEEAGLEEGQDYELLAVGDGGTASVALDRGEISAYAAAFPDVAIMRLRGLELR
                     NLLPEGFQSFFDSLVTVERSFMEENPEIVEGLGRAIAKATLWGMDNPEGVLDITEQYF
                     PEEAEDREFTLAMLQETMSLFELPPEANGQWGYAVPESVDRYMGFLVDQGELPEPVDT
                     SVYVNDYVEAYNAFDPAEL"
     gene            complement(1894..5244)
                     /locus_tag="VF058_12215"
     CDS             complement(1894..5244)
                     /locus_tag="VF058_12215"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6209106.1"
                     /translation="MQVGRRIRNVDWPERTVGSVPFTADVRLPGTLVARVLRSPLPHA
                     RIVSLDVTAAEAAPGVHAVITSEDFGEVKYVHHGGPLADRHPLARRVVRFLGEEIAAV
                     AAESPAQAEAAVALIGLELRPLPAVTTIEEALERGAPEIHSGGNVSLHTERRYGDVAA
                     GRRRATKSVQGRYRFGRQTHVCMETNSTLASWDEEEGVLHLWTSTQAPYFIRKEVAHA
                     LGLELDCVKAHEVAVGGGFGSKSKISDHEVLCAALSKKAGRPVRLVLDRAEDFATTKC
                     RHDWDVRLETGADEEGRLTHRTARILVDNGAYNHSGPSVMGYGSLVMGSFYRTDGVDI
                     DADLVYTNKHPGGQFRGYGGPQATFAIESQMDELADALGMDPIDLRILNANRAGDVTH
                     AGWKLRSARLVECLEAAREAIGWDEKRRLGGSGRGVGIAAAIHVTGAHVYEGAEESHA
                     GVDVRADGTVRVRFGGADAGTWQKTILAQIAAEELGIEAEDVDVLTTETDETPLDLGA
                     WSSRGTFMGGHAVRAAAASAAGRLREAAAKKFGVDPSDVTLTGGQARGGADRLDLGDL
                     VRLASENGELSVEESFTADMEPVNPATGVANLSPTYSFCAQAVEVEVDRDTGQVKLLD
                     VAAAHDSGTIINPIAAESQVIGGVAMGLGAALGEELIYEEGRLVNPAYINYPLPRAAD
                     LPPIRVVFVEGKDPVGPYGAKSLGEIGLVPTQAAVANAVAHAVGVRVRKLPITPDKVL
                     GGLRERDARPRRRYHLWRRPNRWEIALIRWLYPRGLHRLLHRWGTRFARKQEPRAIEE
                     IRAPEEIEEATRLLEDGEAVPIAGGTDLLPARRQGLANPVTLVDLTTVKGMRSILETE
                     YGDLRIGAGVTLADAARTPALDEVLRETIGRIASPQIREMATVGGNLCQQKRCWFYRN
                     GFECYKRGGVTCPCYAVLGDHRFYHAVTKAHRCQAVTPSDLATTLTALDAEAVLARGG
                     ERRTLPMERFFTGPGETALRRGEVLVEVGVPAAARGRKGAFEKLRLWEGDFAVASVAA
                     SLDLGSGGEVRDARIVLGAVAPTPYRAREVEQGLVGRRLHAPTIAEAAEAWTGGAHPL
                     PGNEWKVDATVGLIRRCLSRLGEGESTG"
     gene            complement(5244..5696)
                     /locus_tag="VF058_12220"
     CDS             complement(5244..5696)
                     /locus_tag="VF058_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6209107.1"
                     /translation="MRARIEVNGEVRDLELAPSERLLDVLRDRLGLLGSKEGCGRGEC
                     GACTVLIDGRPVLSCITLAARVRGPVETVEGVADEAVPLREAFADHGAFQCGFCTPGQ
                     IVRGVSLLRGGLPAEEGSLRQLMSGNVCRCTGYAGIVRALRQAESGSR"
     gene            5819..>6344
                     /locus_tag="VF058_12225"
     CDS             5819..>6344
                     /locus_tag="VF058_12225"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6209108.1"
                     /translation="MNIGVGLPNTVPGTEARLIGEWARRIEDGPFTSIGVLDRVRYDS
                     LDPLACLAAITGTTSRVRLVTMVVVGPLRGTTILAKEAATIDVLSGGRLTLGLAVGAR
                     EDDYDAAGVEVRGRGRRFEEQLAALRGVWEDPSIGPRPAQDGGPELLVGGGSDLAFAR
                     VARYADGFVHGGGPP"
BASE COUNT          897 a         2381 c         2148 g          918 t
ORIGIN      
        1 ggacgcctga cggctcgccc tcggctccga cgtcgagcag gcgccgcacc tgccgggcca
       61 gccgctggaa cggctcggtc gtgacgaggt ccagactccg ggggcgcggc aggccgacgt
      121 cgaccacgcc ggccaccctc cccgggcgcg gcgtgatcac gtagacgcga tcggagagga
      181 acaccgcctc ctggatgttg tgggtcacga agatggcggc cttcgcggcg gccgagaaga
      241 tccgcgccag ctcgacgttc atgtgctccc gggtgagctc gtcgagcgcg ccgaacggct
      301 cgtccaggag caggacctcg gggtcggcga tcagcatccg gcagatcgcc acgcgctgct
      361 gcatgccgcc ggacagctcg ctcggatact tgtcctcgaa gcccgccagg tgcaccagct
      421 cgagcagctc gtcggcatgg ggccgcgccg cctctacggc ggcgcggccc ccgcggatct
      481 cgatcgggag aaggacgttg tcccgcgccg tccgccagtc gagcaggacg gggttctgga
      541 acatcatgcc aacctcgggg ggcgggccgt ccaccgggtg ccccttcacc atgaccgtgc
      601 cacgagaggg gtgcagcagg ccccccacga tgttcagcag ggtcgatttg ccgcaaccgg
      661 aggggccgac gagcgcgacg aagtccgtcg gcgccagcga caggtccacg gagcggatcg
      721 cctccacgga cgcgtcgcga cggccgaagc gcttgccgac cccacggacc tcgacgacgg
      781 cctcagtcat cgtcgcgcgg gcgcctcctc agagctcggc cggatcgaac gcattgtagg
      841 cctcaacgta gtcgttcacg tagacgctcg tgtccaccgg ctcgggcagc tcgccctggt
      901 caacgaggaa ccccatgtac cgatccacgg actccggcac cgcgtacccc cactggccgt
      961 tcgcctcggg cgggagctcg aagagcgaca tcgtctcctg cagcatcgcc agggtgaact
     1021 cgcggtcctc ggcctcctcg ggaaagtact gctcggtgat gtcgagcacc ccctcggggt
     1081 tgtccatgcc ccagagggtc gccttggcga tcgctcggcc caggccctcg acgatctccg
     1141 ggttctcctc catgaagctg cgctccacgg tgaccagcga gtcgaagaag ctctggaagc
     1201 cctccggaag caggttgcgg agctcgaggc cccggagccg catgatcgcc acatcgggga
     1261 aggccgccgc gtacgcggag atctccccgc ggtcgagcgc aaccgacgcg gtgccgccgt
     1321 cgcccacggc gagcagctcg taatcctgcc cctcctccaa accggcctcc tcggacagca
     1381 ccgcccggac gaagggcgtc tcgcctccct cgatcgtgcc cacgccgacc gtcgtgccct
     1441 caagatccgc cacggactgc acctgggagt ccgccagggt ctgcagcgag aacacgttcg
     1501 actggaagag cgtgtagacg ctcacgaggt ccgagccctc cagcacgctg ttcatgaacg
     1561 gcccggggct cggcagcccg acctcggcct gacccgccag cacctgctgg aggatctggc
     1621 ccgatccgtc caccgcctcc gtcgtcaccg agagcccctc ctcctcgaag tagcctcggt
     1681 cctgcgcgac gtacaacgga tagaagaccg ccccgctggg aaacgggatc agcgcggtga
     1741 gctccgtgat ctcctggggc tgctcctccc catcgccccc gccgccggcc tcctcgccgt
     1801 cgtcgccgcc acacgcgccg gccaccagca tcagggccag cagcgcgacc agccatcgcg
     1861 accttctcat ctccccctcc ttcgcgtgac gggtcacccc gtcgattcgc cctcccccag
     1921 ccgggagagg cacctccgga tcaggccgac ggtcgcgtcg accttccact cgttgcctgg
     1981 gaggggatgg gcgcccccgg tccaagcctc cgcggcctcg gcgatcgtcg gcgcgtggag
     2041 cctccgaccg acgagcccct gctccacctc gcgtgcgcgg tacggcgtcg gggcgacggc
     2101 gccgagcacg atgcgggcgt cccggacctc gccccccgag cccaggtcga gcgacgcggc
     2161 aaccgacgcc accgcgaagt cgccctccca cagccgcagc ttctcgaacg cccccttccg
     2221 gccccgggcc gcggccggga cgccaacctc gaccaggacc tcgccccgcc gcagggcggt
     2281 ctccccgggc cccgtgaaga agcgctccat agggagggtg cgccgctctc cgccgcgcgc
     2341 gagcacggcc tcggcgtcga gcgcggtcag cgtcgtggcg aggtcggagg gcgtcacggc
     2401 ctggcaccga tgcgccttcg tgaccgcgtg gtagaagcgg tggtccccca gcaccgcgta
     2461 gcacgggcac gtcacgccgc cgcgcttgta gcactcgaag ccgttccggt agaaccagca
     2521 gcgcttctgc tggcacaggt tgccgcccac ggtggccatc tcccggatct ggggcgaggc
     2581 gatgcggccg atcgtctcgc ggaggacctc gtccagagcg ggggtgcggg cggcgtcggc
     2641 cagcgtgacc ccggccccga tccgcagatc gccgtactcc gtctccagga tcgagcgcat
     2701 ccctttgacc gttgtgaggt cgacgagcgt gacggggttg gcgaggccct gtcgtcgagc
     2761 cggaaggagg tcggtcccgc ccgcgatcgg gacagcctcc ccgtcctcga gcagtcgcgt
     2821 ggcctcctcg atctcctcgg gggcgcggat ctcctcgatc gcccgcggct cctgcttcct
     2881 ggcgaacctc gtgccccagc ggtggaggag ccgatggagc ccccgcgggt agagccagcg
     2941 gatcagggcg atctcccagc ggttcggtcg gcgccacagg tggtagcggc ggcgcggccg
     3001 cgcgtcgcgc tcgcgcaggc cccccaggac cttgtcgggc gtgatgggca gcttccggac
     3061 gcgcaccccg accgcgtggg ccaccgcgtt ggccaccgcg gcctgggtcg gcacgagccc
     3121 gatctcgccg aggctcttcg cgccgtacgg tcccacgggg tcctttccct cgacgaacac
     3181 gactcggatc ggggggagat cggccgcccg gggaagcggg tagttgatgt aggcgggatt
     3241 gacgaggcgg ccctcctcgt agataagctc ctcgcccagg gcggcaccga ggcccatcgc
     3301 caccccgccg atcacttggc tctctgcggc gatggggttg ataatcgtcc ccgagtcgtg
     3361 ggcggcggcg acgtcgagga gcttgacctg tcccgtgtcc cggtccacct ccacctccac
     3421 cgcctgggcg cagaaggagt aggtcgggga gaggttcgcc acccccgtgg ccgggttcac
     3481 cggctccatg tccgcggtga agctctcctc cacgctgagc tcgccgtttt cggaggcgag
     3541 cctgacgagg tccccaaggt caaggcggtc cgcgccgccg cgagcctggc ccccggtcag
     3601 cgtgacgtcg gacgggtcca cgccgaactt cttcgcggcc gcctcacgca gcctgccggc
     3661 ggccgaggcc gcggcggcgc ggaccgcgtg cccgcccatg aacgttcccc gggaggacca
     3721 agccccgagg tccagcggag tctcgtcggt ctcggtggtg agcacgtcga catcctcagc
     3781 ctcgatcccc agctcctctg ccgcgatctg ggcgaggatc gtcttctgcc acgtccccgc
     3841 gtcggcccct ccgaagcgca cgcgcaccgt cccgtcggcc cggacgtcga cgccggcgtg
     3901 ggactcctcg gccccctcgt agacgtgagc cccggtgacg tggatcgccg ccgcgattcc
     3961 cacgccccgc ccgctccctc cgagccgccg cttttcgtcc cacccgatgg cctcacgggc
     4021 cgcctcgagg cactcgacca agcgcgccga tcgcagcttc cagccggcgt gggtgacgtc
     4081 gcctgcgcgg ttcgcgttga ggatccgcag gtcgatcggg tccatcccca gggcatcggc
     4141 cagctcgtcc atctgcgact cgatcgcgaa ggtggcctgc ggtcccccat agccccggaa
     4201 ctggcctccc gggtgcttgt tcgtgtagac gaggtcggcg tcgatgtcca cgccgtccgt
     4261 ccggtagaag gaccccatca cgagggagcc gtagcccatc acggagggcc cggagtggtt
     4321 gtaggcgccg ttgtccacga ggatcctcgc cgtgcggtgg gtgaggcggc cctcctcgtc
     4381 ggcgccggtc tctaggcgca cgtcccagtc gtggcggcac ttcgtggtgg cgaagtcctc
     4441 tgctctgtca aggacgagcc ggacgggacg tcccgccttc ttcgacagtg ccgcgcacag
     4501 aacctcgtga tcggagatct tcgacttcga cccgaagccc ccgcccaccg ccacctcgtg
     4561 ggccttcacg caatccagct cgagccccag cgcgtgggcc acctccttgc ggatgaagta
     4621 cggcgcctgg gtggacgtcc agaggtggag gacgccctcc tcctcgtccc agctggcgag
     4681 tgtgctgttc gtctccatgc agacatgggt ctgccggccg aaccggtagc ggccttggac
     4741 cgacttggtc gcccggcggc gccccgcggc gacatctccg tagcggcgct ccgtgtggag
     4801 cgagacgttg ccgccggagt ggatctcggg ggcgccccgc tccagcgcct cctcgatcgt
     4861 cgtgacggcg gggaggggcc ggagctcgag gccgatcagg gcgaccgctg cctcagcctg
     4921 cgcgggagac tccgcggcca cggccgcgat ctcctccccg agaaagcgca ccacgcggcg
     4981 ggccaaggga tggcggtcgg cgagggggcc tccgtgatgc acgtacttca cctcgccgaa
     5041 atcctcggag gtgatcacgg cgtgcacgcc cggggcggcc tcggccgccg tcacgtcgag
     5101 cgagacgatc cgcgcgtgtg gcaagggact tcgcagcacc ctcgccacga gcgtacccgg
     5161 gagtcggacg tcggccgtga aggggacgga gcccaccgtc cgctccggcc agtccacgtt
     5221 gcggatccgg cggcccacct gcatcagcgc gacccactct cggcttggcg gagcgcccgc
     5281 acgatccccg cgtacccggt gcaccgacag acgttgcccg acatcagttg gcggagcgat
     5341 ccctcctccg ccggcaggcc cccgcgcagg agggagacgc cccgaacgat ctgccccggc
     5401 gtgcagaacc cgcactggaa ggcgccgtga tccgcgaacg cctcgcggag gggcactgcc
     5461 tcatcggcca ccccctcgac ggtctccacc ggaccgcgca ccctcgcggc caacgtgatg
     5521 caggaaagga cggggcgtcc gtcgatcaac acggtgcagg cgccgcactc gccgcggccg
     5581 cagccctcct tgcttccgag gagccccagg cggtcccgca gcacgtcgag caaccgctcg
     5641 ctcggagcga gctcgaggtc gcggacctcc ccgttcacct cgatcctcgc cctcacgagt
     5701 cctccgtcat gcgctccggc ctgatgactg acaacagtct cctgattatg ttgagcggag
     5761 cggggttggg caaggcggag ggcctagggt atcgtccccg cacgagggag gccagcggat
     5821 gaacatcggg gtgggacttc cgaacacggt tccggggacg gaggctcgac tcatcggcga
     5881 atgggcccgc cggatcgagg acgggccctt caccagcatc ggggtcctcg accgcgtgcg
     5941 gtacgactcc ctcgatcccc tggcctgcct ggccgcgatc acgggcacga cctcccgtgt
     6001 gcgactcgtc acgatggtcg tcgtgggccc tctgcgaggc accaccatcc tggccaagga
     6061 ggcggcgacg atcgacgtcc tctcgggggg ccggctgacg ctgggcctcg cggtcggcgc
     6121 ccgcgaggac gactacgacg cggcgggcgt cgaggtccgg ggccgagggc gccggttcga
     6181 agagcagctc gccgcgctcc ggggcgtctg ggaggacccc tcgatcggtc cccgccccgc
     6241 ccaggacggc ggccccgagc tcctcgtcgg aggaggcagc gatctcgcct tcgcgcgcgt
     6301 cgcccggtac gccgacggat tcgtccacgg cggcggtccg cctc
//
LOCUS       DASUTW010000362         3054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_129417, whole genome shotgun sequence.
ACCESSION   DASUTW010000362 DASUTW010000000
VERSION     DASUTW010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129417"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /gene="rnpB"
                     /locus_tag="VF058_12230"
     ncRNA           complement(<1..134)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VF058_12230"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(152..640)
                     /locus_tag="VF058_12235"
     CDS             complement(152..640)
                     /locus_tag="VF058_12235"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEX6209109.1"
                     /translation="MRLLSPDPHPHLIVHTDGAARGNPGPAGIGAIVTDGSGRTVATL
                     AEGIGVATNNQAEYRAVIEGLRLAAERGAKRVLVRSDSKLLVEQLSGRFRVKNPTLQR
                     LHAEVRALVRTFEGVDFEHVRRGENAEADALANEGVDDWLEGPGRAYEPADPRPPELF
                     EE"
     gene            complement(604..1305)
                     /locus_tag="VF058_12240"
     CDS             complement(604..1305)
                     /locus_tag="VF058_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEX6209110.1"
                     /translation="MDRLLELQELDLSVVRLEARREEIESGKELAEARRRQEELEGRL
                     GEIRMALDGVKGEQARLENDVSMLTQKAEAEERRLYDGSVANPKELESIQAELKNLRD
                     RRSRLEDGILEQMERREQLEGGLPQLEAEVEEARARVAEIETSSETELEQIGAALAER
                     AGERAALVQEIDEELLDLYEDLRSKKKGVGAAALVDGVCQGCRQQLSPLELDRLKKVD
                     GVRRCDYCRRILILT"
     gene            complement(1311..2423)
                     /locus_tag="VF058_12245"
     CDS             complement(1311..2423)
                     /locus_tag="VF058_12245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX6209111.1"
                     /translation="MPGSPTAGGWIAALDELYPPAWAEAWDNVGLQVGDRAWPARRVL
                     VALDPTEEVVAEAVERGCGLVVTHHPLLFRPRSSLDLRDPVSRTAASAIRAEVAIVAC
                     HTNADVAMPGVSDTLAEALGIEVAGVLQGTGAGGRAKLVTFVPPESTAKVLDAVAAAG
                     AAVIGEYTHCSFRVTGTGTFLPSEAADPLIGERGELNQVEEDRVEIVVLQERLGPVIE
                     ALLQAHPYDEVAYDVYPLAGPTGLGLGRVGRLPDPITAGDLALACERELGARPRLVGD
                     PRRPVEQVAVCGGSGASLIPDALAVRVHAFVTGDVKHHDAQDAAAAGLIVIDAGHHAT
                     EWPFVPRLAERLAEPAAALGGDVVVSDVDTDPFRTP"
     gene            2520..>3054
                     /locus_tag="VF058_12250"
     CDS             2520..>3054
                     /locus_tag="VF058_12250"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX6209112.1"
                     /translation="MRGWMRVAIAAGIVAGAVPIAVAGATTAASCKGRPATIVGTAGH
                     DTIVGTPGNDVIAAKGGDDLVRGRGGRDRICGGGGVDTLHGGPGRDFLFGGAGNDLLR
                     GGGRKDLVNGGAGNDFGYGGAGPDDMFGLGGRDVLRGQGGADFIFGDFGNDELYGNRG
                     NDCITTADGTSGNDLGKG"
BASE COUNT          392 a         1117 c         1129 g          416 t
ORIGIN      
        1 ttacctcccg agggaggcgg tctgctctct gtggcacttt ccgcgggtcg ccccgcctgg
       61 gtgttaccca gcaccctgcc ctgtggagtc cggactttcc tcaccccgaa gggcgcggcc
      121 gcccggcccg ctcctcgtac cgatccccag tctactcctc gaacagctcc ggcggccggg
      181 ggtcggcggg ctcgtacgct cgccccggcc cctccagcca gtcgtccacg ccctcgttgg
      241 ccagggcatc ggcctcggcg ttctcgcccc gccggacgtg ctcgaagtcc accccctcga
      301 aggtgcggac gagcgcccgg acctccgcgt gcagccgctg gagggtgggg ttcttcaccc
      361 ggaagcgtcc ggagagctgc tcgacgagga gcttgctgtc cgagcggacg agcacgcgct
      421 tcgccccgcg ctcggcggca aggcggagcc cctcgatcac ggcgcggtac tcggcctggt
      481 tgttcgtggc caccccgatc ccctcggcca gcgtggccac cgtgcggccc gacccgtcgg
      541 tgacgatggc cccgatgccc gccgggccgg ggttgccccg ggccgcgccg tcggtgtgga
      601 cgatcaggtg aggatgagga tccggcgaca gtagtcgcac ctccggacgc cgtcgacctt
      661 cttgaggcgg tccagctcca ggggggagag ctgctggcgg cagccctggc acacgccgtc
      721 cacgagggcc gccgccccca cgcccttctt cttcgaccgg aggtcctcgt agaggtcgag
      781 cagctcctcg tcgatctcct ggacgagggc ggcgcgctcg cccgcccgct ccgccagcgc
      841 cgccccgatc tgctcgagct cggtctccga ggaggtctcg atctccgcga cgcgggcccg
      901 ggcctcctcg acctcggcct cgagctgggg caggcccccc tccagctgct cccgccgctc
      961 catctgctcc aggatcccgt cctcgaggcg ggatcgccgg tcgcgcaagt tcttcagctc
     1021 ggcctggatc gactcgagct ccttggggtt ggccacgctg ccgtcgtaga ggcgccgctc
     1081 ctccgcctcg gccttctggg tcagcatcga gacgtcgttc tccagccgcg cctgctcacc
     1141 cttgaccccg tccagggcca tgcggatctc gccgaggcgc ccctcgagct cttcctggcg
     1201 tcgtcgcgcc tcggccagct ccttccccga ctcgatctcc tcgcggcgag cctcgaggcg
     1261 gaccacggac aggtcgagct cctgaagctc gaggaggcga tccatcccct tcacggcgtg
     1321 cggaaggggt cggtgtccac gtcggagacg accacgtcgc ccccgagggc ggcggcgggc
     1381 tcggccaggc gctcggcgag gcgggggacg aagggccact cggtggcgtg gtgaccggcg
     1441 tcgatcacga tgaggccggc cgcggcggca tcctgggcgt cgtggtgctt gacgtcgccg
     1501 gtcacgaaag cgtggacgcg cacggcgagc gcgtcgggaa tcagggaggc gccggagccg
     1561 ccgcagacgg ccacctgctc gacggggcgg cggggatcac ccaccaggcg ggggcgggcg
     1621 cccagctcgc gctcgcaggc gagggcgagg tcgcccgcgg tgatcgggtc cgggaggcgg
     1681 ccgacgcgac cgagcccgag cccggtcggt cccgccagcg ggtagacgtc gtaggcgacc
     1741 tcgtcgtagg gatgggcctg gagcagggcc tcgatcacgg ggccgaggcg ttcctgaagg
     1801 accacgatct cgacgcgatc ctcctcgacc tggttcagct ccccgcgctc cccgatcagc
     1861 gggtcggcgg cctcggaggg gaggaaggtg ccggtgccgg tcacgcggaa ggagcagtgc
     1921 gtgtactcgc cgatcacggc ggcgccggcg gccgcgaccg cgtcgagcac cttggcggtc
     1981 gactccggcg ggacgaacgt cacgagcttg gcgcggccgc cggcgccggt cccctggagc
     2041 accccggcga cctcgatccc cagcgcctcg gccagcgtgt ccgacacccc gggcatcgcg
     2101 acgtccgcgt tcgtgtggca ggccacgatc gcgacctcgg cccggatcgc gctcgccgcc
     2161 gtccggctca ccggatcccg gaggtcgagg ctcgaccggg gccggaacag cagcgggtgg
     2221 tgcgtcacga cgagcccgca tccccgctcc accgcctcgg ccacgacctc ctcggtgggg
     2281 tccagggcca cgagcacgcg ccgcgcaggc caggctcgat ccccaacctg gagccccacg
     2341 ttgtcccagg cctcggccca ggcgggtggg tacagctcgt ccagggcggc gatccatcct
     2401 ccggcggtcg ggcttcctgg catgcgagcc gtagcatagc gcccgccgcc gggaccggac
     2461 cttcccgtga cgagacgacg ggtgtacgct tcgccgcgct cggacgagag gagggcggga
     2521 tgcggggatg gatgcgggtg gcgatcgccg ccgggatcgt ggccggggcg gtcccgatcg
     2581 cggtcgcggg cgccacgacg gcggcgagct gcaagggccg gccggccacg atcgtcggga
     2641 cggcagggca cgacacgatc gtcggcacgc cggggaacga cgtgatcgca gccaagggcg
     2701 gcgacgacct ggtgcgcggc cgcggcgggc gggaccggat ctgcggcggc gggggcgtgg
     2761 acacgctcca cggcggcccg gggcgggact tcctgttcgg gggcgccggg aacgacctat
     2821 tgaggggcgg cgggagaaag gacctcgtga acggaggggc ggggaacgac ttcgggtacg
     2881 gtggcgcggg ccccgacgac atgttcggcc tcgggggccg cgacgttctt cgcgggcagg
     2941 gcggcgccga cttcatcttc ggcgacttcg ggaacgacga gctctacggc aaccgaggca
     3001 acgactgcat cacgaccgcc gacggcacga gcggcaacga cctcgggaag ggag
//
LOCUS       DASUTW010000363         4813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1423284, whole genome shotgun sequence.
ACCESSION   DASUTW010000363 DASUTW010000000
VERSION     DASUTW010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423284"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1594)
                     /locus_tag="VF058_12255"
     CDS             complement(<1..1594)
                     /locus_tag="VF058_12255"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX6209113.1"
                     /translation="MKLVRRLSVVTAIATLGLVALGGVVRATGSGLGCPDWPRCHGRL
                     IPPLEFHALIEYSHRLLASIVVILVIATAWAAWRRARGTPQVLRPAVLAIGVVLLQAG
                     LGAVVVAEELAPLPNTLHFATAMLLVATVVTTAANAVLGRAAPGDGPRGAPGMRRLLW
                     WALGVTAGALLAGVYMRGSGSSLAFLDWPLMDGRAIPELRTEAAWAAFLHRAIAALAV
                     VLIVGIAVRARELAPRLRWLAWTAAGLVLLQSGLGAATVFTRLDALPVAGHILGSSLT
                     WASIVALVAGTRPVRHAEAVEESDRPGARQVAGAYLQLMKPDIIVLLLVTTVPTMILA
                     AGGFPPVGLVAATLLGGTLAAGGANALNHYLDRDIDEVMDRTRARPIPAHRVEPDDAK
                     SFGILLSGLSFAWLALTVNLLSAALALSAILFYVFVYTAWMKRSTPQNIVIGGAAGAV
                     PALVGWAAVTGSLALPAWVLFALIFFWTPPHFWALSMKYADDYQAAGVPMLVVLHGSA
                     DTATHILLYSIQTVAVSLLLFPA"
     gene            1656..2615
                     /locus_tag="VF058_12260"
     CDS             1656..2615
                     /locus_tag="VF058_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX6209114.1"
                     /translation="MTRTTTLALALALGLVSAACGSDGDEGPQAGPSPSPGGGELVAQ
                     VASYDLATGTTDRFIVGLLTQDNRFVSGGEATFRFSFIGGGQPEPGPEVTAEFLAIPG
                     EDPEHEAAHAGPPAEGRGVYAVRDVTFDRAGIWQVEVIADVDGEPLSGTAAFQVAGEH
                     AYPVVGDRAPRTDNLTLDDIGEAPPGAVDSRAEGGEEVPDPELHDSTIAQAIRRGRPI
                     VAVFATPVYCLSQFCGPITDMIAELESEYRDRAEFVHVEVWRDFQNNVVNRAAADWIL
                     RNDNILEPWVFFVDADGVIRGRWDNVATREEVEALLRDLPPMG"
     gene            2624..3706
                     /locus_tag="VF058_12265"
     CDS             2624..3706
                     /locus_tag="VF058_12265"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX6209115.1"
                     /translation="MRVADVPLPAACLDLAAAVEEDLARFLQGERAEAEERAPGVGEL
                     FDELERVIAAGGKRLRPVFCLLGHRAAGGEVGPVVIRAAASLELLHTFAIVHDDVMDG
                     SRTRRGAPATWAHLEGLHAAERMRGSAEVFGVSGAILAGDLALILADGAFLGAGLAPG
                     ALLPALARYNRMRVEVVAGQYLDVAAAHRGEAAEPEARRIAGLKSGNYTVEGPLQIGA
                     ILAGGSGELLAALSRYGMPLGEAFQLRDDILGVFGDPALTGKPRDSDLREGKRTVLIA
                     RALERAEHEEAAFLLERLGAPDLTDADVERIRGIIESSGALDAVGGLVAELADRARAA
                     LDVAGLPGDVRAALADLVETCTLRIS"
     gene            3754..4212
                     /locus_tag="VF058_12270"
     CDS             3754..4212
                     /locus_tag="VF058_12270"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6209116.1"
                     /translation="MRLLDARRIFGDLDVVHLATTLADGSPHVVPLWFVWREEAIYVS
                     ARRDSVTWRNVERDPRAALTFSRGSEWRDLAGIVIHGRAEPLSAVHPALRGVMSSWYE
                     KYREALSGEGFRAFAEQVDRPGMMRVRPLHMAAWDHALWLPGQRSGDAAG"
     gene            4253..>4813
                     /locus_tag="VF058_12275"
     CDS             4253..>4813
                     /locus_tag="VF058_12275"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX6209117.1"
                     /translation="MNGFTSSAERVDVLVVGAGPAGSAAAAHLARHGVDVLAVDKAAF
                     PREKVCGDGLTPRGVKQLLELGIDVEERGFVRIDGLRSFGPGVKVELPWPESDAFPSF
                     GLVRTRHDLDELLIRNAEKSGAIVWQETDAERPVLDRWGRVLGAVVRRGEGEPREVRA
                     RYVIAADGASSRFAQQAGVRRDPDRPI"
BASE COUNT          651 a         1714 c         1844 g          604 t
ORIGIN      
        1 ccgccgggaa gaggagcagc gacaccgcca cggtctggat cgagtacagg aggatgtggg
       61 tcgccgtgtc ggcgctgccg tggaggacga cgagcatcgg gaccccggcc gcctggtagt
      121 cgtccgcgta cttcatcgac agcgcccaga agtggggggg cgtccagaag aagatcaggg
      181 cgaagaggac ccacgccggc agggccaggc tcccggtcac cgccgcccac cccacgagcg
      241 ccggcaccgc gccggccgcc ccgccgatca cgatgttctg cggcgtggag cgcttcatcc
      301 acgccgtgta gacgaagacg tagaagagga tcgccgagag cgccagcgct gccgacagca
      361 ggttcaccgt cagcgccagc caggcgaagg acagccccga gaggaggatc ccgaagctct
      421 tcgcgtcgtc gggctccacg cggtgcgcgg ggatgggtcg ggcccgggtc cggtccatca
      481 cctcgtcgat gtcccggtcg aggtagtggt tcagggcgtt ggcgcctccc gccgccagcg
      541 tccccccgag gagcgtggcg gccacgaggc ccacgggcgg gaagcctccg gccgcaagga
      601 tcatcgtcgg gacggtcgtg acgagcagga gcacgatgat gtcgggcttc atcagctgga
      661 ggtaggcgcc ggccacctgc cgtgcgccgg gccggtcgct ctcctcgacc gcctccgcat
      721 gccgcaccgg gcgcgtgccg gcgacgagcg ccacgatgga cgcccacgtg aggctgctgc
      781 ccaggatgtg gccggccacc ggcagagcgt ccagccgggt gaagaccgtg gcggcgccca
      841 gccccgactg gagcaggacc agaccggcgg ccgtccacgc gagccaccgc agccgcgggg
      901 cgagctcgcg ggcccgcacg gcgatgccga cgatcagcac gacggccagg gcggcgatcg
      961 cccggtggag gaaggcggcc caggccgcct cggtccggag ctcggggatc gccctcccgt
     1021 ccatcagcgg ccagtccagg aatgcgaggc tcgagcccga gccgcgcatg tacacgccgg
     1081 ccagcagcgc gcccgccgtg accccgagcg cccaccacag caggcgccgc atgcccgggg
     1141 cgccgcgagg accgtcgccg ggcgccgcgc gtcccagcac cgcgttcgct gcggtcgtga
     1201 cgaccgtggc cacgagcagc atcgccgtgg cgaagtggag ggtgttgggg aggggggcca
     1261 gctcctcggc cacgaccacc gcgccgaggc ccgcttggag caggacaacg ccgatcgcca
     1321 gcacggccgg ccgcaggacc tgcggcgtcc cgcgggcccg gcgccacgcg gcccacgccg
     1381 tggcgatgac gaggatcacc acgatcgagg ccagcagtcg atggctgtac tcgatcagcg
     1441 cgtggaactc gagcggcggg atcagccgcc cgtggcaccg gggccagtcg gggcatccca
     1501 gccccgagcc ggtggcgcgg accacgccgc ccagcgcgac cagtcccagc gtggcaatgg
     1561 cggtgacgac ggagaggcgg cgaacgagct tcatccgtgc cgtcatcata ctcacggcgc
     1621 cgcggaccca cgccgcccgc gctacattgg ggatcgtgac ccgaaccacg acgctcgcgc
     1681 tggcgctcgc cctgggcctg gtctccgcgg cctgcggatc ggacggggac gaggggccgc
     1741 aggccggccc gtccccgagc ccgggcgggg gcgagctcgt ggcccaggtc gcgagctacg
     1801 atctcgccac cggcaccacc gaccggttca tcgtgggcct cctcacccag gacaatcgct
     1861 tcgtctcggg cggcgaggcg accttccgct tctccttcat cgggggaggg cagcccgagc
     1921 ctggccccga ggtcaccgcg gagttcctgg ccatccccgg cgaggacccc gagcacgagg
     1981 ccgcccacgc cggcccgccg gccgagggcc ggggcgtcta cgcggtccgc gacgtgacct
     2041 tcgaccgcgc cggcatctgg caggtcgagg tgatcgccga tgtggacggc gagccgctct
     2101 cggggacggc ggccttccag gtggcgggcg agcacgcgta cccggtcgtc ggcgaccggg
     2161 cgccccggac cgacaacctc acgctcgacg acatcgggga ggctcccccg ggagccgtcg
     2221 actccagggc cgaagggggc gaggaggtcc ccgacccgga gcttcacgac tccacgatcg
     2281 cgcaggcgat ccggcggggg aggcccatcg tggccgtgtt cgccaccccc gtctactgcc
     2341 tgagccagtt ctgcggcccg atcacggaca tgatcgccga gctcgagtcc gagtaccgcg
     2401 accgggccga gtttgtccac gtcgaggtgt ggcgggactt ccagaacaac gtcgtgaacc
     2461 gggccgccgc cgactggatc ctgcggaacg acaacatcct cgagccctgg gtcttcttcg
     2521 tggacgccga cggcgtgatc cgggggcgct gggacaacgt cgccacccgc gaggaggtcg
     2581 aggccctcct ccgggacctc cccccgatgg gctgatgccg ggcgtgcgcg tcgccgacgt
     2641 ccccctgccg gcggcgtgcc tcgacctcgc ggccgcggtg gaggaggacc tggcccgctt
     2701 cctccagggc gagcgggccg aggccgagga gcgggcgccg ggcgtggggg agctcttcga
     2761 cgagctggag cgggtgatcg ccgccggcgg gaagcggctc cggccggtct tctgcctcct
     2821 gggccatcgg gcggcggggg gcgaggtcgg ccccgtggtg atccgggcgg cggcctcgct
     2881 ggagctgctc cacaccttcg ccatcgtcca cgacgacgtg atggacgggt cccggacgcg
     2941 gcgcggagct ccggccacgt gggcccacct ggagggcctc cacgccgccg agcggatgcg
     3001 ggggagcgcg gaggtcttcg gcgtgtcggg ggcgatcctg gccggcgacc tggcgctgat
     3061 cctcgccgac ggcgcgttcc tcggcgccgg gctcgcgccg ggagcgctgc tcccggcgct
     3121 cgcccgctac aaccggatgc gggtggaggt cgtggccggc cagtacctcg acgtggcggc
     3181 ggcgcaccgg ggggaggcgg ccgagcccga ggcccgccgg atcgcggggc tgaagtccgg
     3241 caactacacg gtggaggggc cgctccagat cggggcgatc ctggccgggg gctcgggcga
     3301 gctgctggcg gccctgagca ggtacgggat gccgctgggc gaggccttcc agctgcgcga
     3361 cgacatcctc ggggtcttcg gggaccccgc gctgaccggg aagccccggg actcggacct
     3421 tcgcgaggga aagcggaccg tgctgatcgc ccgggctctg gagcgagcgg agcacgaaga
     3481 ggcagcgttc ctcctggaac ggctcggggc gcccgacctg accgatgcgg acgtcgagcg
     3541 gatccgcggg atcatcgagt cgtcgggggc cctcgacgcg gtggggggcc tcgtggccga
     3601 gctggccgac cgggctcgcg ccgcgctgga cgtcgccggg ctccccggcg acgtccgcgc
     3661 ggcgctcgcc gacctcgtgg agacgtgcac cctgcgcatc tcgtgatcgg gtgattgatt
     3721 cgatcgcccg cgtgtgggac aactagccgg gccatgcggc tgctcgacgc tcgcaggatc
     3781 ttcggggatc tcgacgtggt ccacctcgcg accaccctcg ccgacgggag cccccacgtc
     3841 gtcccgctct ggttcgtctg gcgggaggag gcgatctacg tctcggcccg acgcgactcg
     3901 gtgacctggc gcaacgtcga gagggaccct cgcgccgccc tcaccttcag ccgcgggagc
     3961 gagtggcggg acctcgccgg gatcgtgatc cacggccggg cggagccgct ctcggccgtc
     4021 caccccgcgc tccgcggcgt gatgtcctcc tggtacgaga agtaccgcga ggcgctgtcg
     4081 ggggagggct tccgggcgtt cgccgagcag gtggaccggc cggggatgat gcgggtccgc
     4141 ccgctccaca tggccgcgtg ggaccacgcc ctctggctcc ccggccagcg gtccggcgac
     4201 gcggccggct gaagcgtttg cgagatacct ggggcgggcg tagcatccgc ttgtgaacgg
     4261 tttcacaagc tccgccgagc gggtggacgt gctggtcgtc ggggccggcc ccgcgggatc
     4321 cgcggccgcg gcgcatctcg cccggcacgg cgtggacgtg ctcgcggtgg acaaggcggc
     4381 cttcccccgc gagaaggtct gcggggacgg cctgaccccc cgaggcgtga agcagctcct
     4441 cgagctcggg atcgacgtcg aggagcgggg cttcgtgcgc atcgacggcc tgaggtcctt
     4501 cggtccgggg gtgaaggtcg agctcccctg gcccgagtcg gacgccttcc ccagcttcgg
     4561 gctggtgcgg accaggcacg acctcgacga gctcctgatc cggaacgccg agaagtcggg
     4621 ggcgatcgtg tggcaggaga ccgatgccga gcggccggtc ctggaccgat gggggcgtgt
     4681 gctcggggcc gtggtgcgga ggggcgaggg cgagccccgg gaggtccggg cccggtacgt
     4741 gatcgccgcg gacggggcgt cgtcgcggtt cgcccagcag gcgggggtgc gccgcgatcc
     4801 cgatcgtccg atc
//
LOCUS       DASUTW010000364         2604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1295283, whole genome shotgun sequence.
ACCESSION   DASUTW010000364 DASUTW010000000
VERSION     DASUTW010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295283"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..1999)
                     /locus_tag="VF058_12280"
     CDS             complement(119..1999)
                     /locus_tag="VF058_12280"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEX6209118.1"
                     /translation="MPRSDDRSTDRGLPSRIVAHIHRDVPLVLLDATLILIAYLVPLA
                     LRFEGEVPADYWASFWLLFPAIGGIHLACNLLFGLYGEMWRYASVEEARRVALAGIVA
                     GGLVIMGVMLLPDRPLPLSVVMLGAILALIGFGTVRFQSRLFAFKRRTEPEEQHRVLL
                     VGAGQAGAMVSKDLRTNRHLGLRPVGFVDDDPRKIGLALQEVRVLGPREAIPSLVERL
                     RVDQVLLTIPSATSEVVRDVAALCEQADVTLKVLPSVRDIMGGKVAARDIRDLRIEDL
                     LGRSQADIDLEAVASFLRGRRVLVTGAGGSIGAEICRQVAALDPAALTLLDHDETHLH
                     DVLTELEGDVPIETTLVDIRDRERVIRAMMRHRPQVVFHAAAHKHVPLLEIHPEEAVL
                     TNVMGTANVVDAAVAAHAERFVLISTDKAVKPVSVMGASKRLAEQIARSALGNGSIFC
                     AVRFGNVLGSRGSVIPTMFRQIARGGPVTVTHPAMSRYFMSIQEAVELVLQAAALAEG
                     GEVFTLEMGEPVNILDMAKRIIRLSGRVPGKDVQISITGARPGEKLVEDVIDPDEELL
                     SSAHPDIMVSRPVPPSRSALHAAIGELDVMVRDGRTGDLADRIKAVALGLPVPLDAEA
                     AS"
     gene            2180..2539
                     /locus_tag="VF058_12285"
     CDS             2180..2539
                     /locus_tag="VF058_12285"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX6209119.1"
                     /translation="MDRELPRPEARSGEPTPEGLSVPLSYVPHRVPTVSELEVGDGLI
                     LYVEEADLIHRLNASAELVWRLCEGEATVGQLSGEIAEEYGLDVDEVSIQIASLVGEL
                     DALGLVSGQPEADRPTE"
BASE COUNT          400 a          898 c          910 g          396 t
ORIGIN      
        1 ccctcggcag tgtcggtggc gatgcccacc ggcacgagcc aggggtacct ggagagggcc
       61 gcagcgatgc cctcggccac catggcgagc cgatggatca ccacgatcgt gcgggaggtc
      121 aactcgccgc ctccgcgtcg aggggcacgg ggaggcccaa ggcgacggcc ttgatgcgat
      181 ccgcgaggtc gccggtcctc ccgtccctca ccatcacgtc gagctcgccg atcgccgcat
      241 ggagggccga gcggctcgga gggaccgggc gcgacaccat gatgtccggg tgcgcgctgg
      301 agaggagctc ctcgtcgggg tcgatcacgt cctcgacgag tttctcgccg gggcgcgcgc
      361 cggtgatcga gatttggacg tccttgccag ggacgcgtcc ggagagcctg ataatgcgct
      421 tggccatgtc caggatgttg accggctccc ccatctccag ggtgaacacc tctcccccct
      481 cggccagcgc ggccgcctgg agcacgagct ccacggcctc ctggatgctc atgaagtagc
      541 gcgacatcgc cggatgggtg acggtcacgg gccctccccg ggcgatctgc cggaacatcg
      601 tggggatcac gctcccccgg ctccccagga cgttcccgaa tcggaccgcg cagaagatgc
      661 tcccgttccc aagagcgctg cgggcgatct gctcggcgag cctcttcgac gcccccatga
      721 cgctcacggg cttgaccgcc ttgtcggtcg agatgaggac gaagcgctcg gcgtgcgccg
      781 ccaccgccgc atcgaccacg ttggccgtcc ccatcacgtt ggtgaggacc gcctcctctg
      841 ggtggatctc gagcaggggg acatgcttgt gcgccgccgc gtggaaaacg acctgggggc
      901 ggtggcgcat catggcccgg atcacgcgct cccggtcgcg gatgtccacg agcgtcgtct
      961 cgatcggaac gtcgccctcg agctcggtca acacgtcgtg gaggtgcgtc tcgtcgtggt
     1021 cgaggagcgt cagcgccgcg ggatcgagcg ccgcgacttg gcggcagatc tccgccccga
     1081 tggagccgcc ggcgccggtc accagcacgc gccggccccg caggaacgag gcgacggcct
     1141 cgaggtcgat gtcggcctgg ctgcggccca ggagatcctc gatccgcagg tcgcggatgt
     1201 cacgggccgc caccttcccg cccatgatgt ctcggaccga gggcaggacc ttgagggtca
     1261 cgtcggcctg ctcgcatagc gcggcgacgt cgcggacgac ctcgctggtc gccgatggga
     1321 tcgtgagcag cacctggtcc acccggagtc gctcgaccag cgaggggatg gcctcacggg
     1381 ggccgaggac gcgaacctcc tggagggcca gtccgatctt ccgtgggtcg tcgtctacga
     1441 accccacggg acggagcccg aggtgccggt tggtccggag gtccttggag accatcgccc
     1501 ccgcctggcc agccccgacc agcagcacgc gatgctgctc ctcgggctcg gtccgtcgct
     1561 tgaaggcgaa gagacggctc tggaaccgga cggtgccgaa cccgatcagc gccaggattg
     1621 cgccgagcat gacgaccgag agggggagcg gtcggtccgg gaggagcatg actcccatga
     1681 tcacgagccc gcccgccacg atgccggcca gggctacacg cctggcctcc tccacgctcg
     1741 cgtagcgcca catctctccg taaaggccga agaggaggtt gcaggcgagg tggatccctc
     1801 ctatggccgg gaagaggagc cagaacgacg cccagtagtc cgccggcacc tcgccctcga
     1861 agcggagggc cagcgggacc agataggcga tcaggatgag ggtcgcgtcg aggagcacga
     1921 gcgggacgtc gcggtggatg tgtgcgacga tcctcgaagg aagcccccgg tccgtcgacc
     1981 ggtcgtcgga acgcggcacg ccttcctccc ccgcgggcga tctccctggc atcggtcgaa
     2041 cagcgtaggt cgaaggtctg cgtacctcat cggtcgggtc tcgtatcttc tctacgcctg
     2101 atgcagtacg gggtcggtcg ccttatctac gtctcctgcg ggatgccctc cggccagccc
     2161 ggcccgacac tggctcttcg tggaccgaga actaccgcgt ccggaggcca ggagcggcga
     2221 gccgaccccc gaggggctct ccgtgccgct ctcctacgtc ccccaccggg tccccaccgt
     2281 ctccgagctc gaggtgggcg atgggctcat cctctacgtg gaggaggcgg acctcatcca
     2341 ccggctcaac gcgtcggccg agctcgtctg gcggctgtgc gagggggagg cgacggtggg
     2401 gcagctctcg ggcgagatcg cggaagagta cgggctggac gtcgatgagg tgtcgatcca
     2461 gatcgcgtcg ctggtcggag agctcgacgc gctggggctg gtttccgggc agccggaggc
     2521 cgaccgcccg accgagtgac tagacccccc tacgccctgc atcgtagaga cccgatccgt
     2581 cacccctctg acctgtggcg ctgg
//
LOCUS       DASUTW010000365         2222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1773
            k141_1165870, whole genome shotgun sequence.
ACCESSION   DASUTW010000365 DASUTW010000000
VERSION     DASUTW010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961106
            Sequence Read Archive: SRR25158390
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:43:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,460
            CDSs (total)                      :: 2,422
            Genes (coding)                    :: 2,402
            CDSs (with protein)               :: 2,402
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 20 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165870"
                     /isolate="SMAG_U1773"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.27666667 N 117.4744444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VF058_12290"
     CDS             <1..541
                     /locus_tag="VF058_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HEX6209120.1"
                     /translation="TRLISKTSAGAPADDDSDDPAISANGRFVAFESNATNLPAGALH
                     ADDMVYVHDRKTRKTRLVSKSSTGAPADADADDASISPEGRFVAFESSADNLPGGAVA
                     TDDLAYVHDRKSGKTVLVSKAANGAPANDFAGIPSASRALSSRGAFVVFTTQATNLPG
                     WDVGSNELVYIRGALRWPR"
     gene            545..805
                     /locus_tag="VF058_12295"
     CDS             545..805
                     /locus_tag="VF058_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6209121.1"
                     /translation="MGPASREGAHASDSAGRHVAGKKTWLVSKSNAGEPGDAFTGIPA
                     NQRVLSSGGRFVVFDTEATNLPGGGAPANRLVYVRGPLRWPR"
     gene            847..2139
                     /gene="hemL"
                     /locus_tag="VF058_12300"
     CDS             847..2139
                     /gene="hemL"
                     /locus_tag="VF058_12300"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX6209122.1"
                     /translation="MTRSEDLFERARAVIPGGVSSPVRAFGAVGGVPRFAASGEGAWV
                     EDVDGARRIDYVQSWGALLFGHARPEIVEAAREAAGRGTSFGMPTEGEVRLAEALRAA
                     MPSLEMVRLVSSGTEAAMSAVRLARAGAGRDRVVKFAGCYHGHSDGLLASAGSGLATF
                     GIPGTPGVTEGASRDTLVLPYNDREALAGAFEAVGDEIAAVIVEPVAANMGVVPPEPG
                     FLEDLRDLTIRSGALLIFDEVITGFRVGRGGAQERFGVTPDLTVLGKVMGGGMPMAAF
                     GGRAEIMERLAPAGDVYQAGTLSGNPVAVAAGLAALDLIERRDPYPGLEKAAERLVEG
                     LASILEDAGIPFTINRAASLFSLFFGVEGVRDHAQAAAADHEAYARFFHGMLEAGVAL
                     PPSGYEGWFLGTAHGDDEIERTLEAAASAAERVSAPSR"
BASE COUNT          309 a          777 c          834 g          302 t
ORIGIN      
        1 gacccggctg atcagcaaga cctcggccgg ggctcccgcc gacgacgact cggacgaccc
       61 ggcgatctcc gccaacggtc ggttcgtggc gttcgagtcc aacgccacca accttccggc
      121 cggcgccctg catgccgacg acatggtcta cgtccacgac cggaagacga ggaagacccg
      181 cctggtcagc aagagctcga ccggcgcgcc cgccgatgcc gacgccgacg acgcgtccat
      241 ctcccccgag ggacggttcg tggcgttcga gtcgagcgcc gacaacctgc cgggaggggc
      301 cgtggccacc gacgacctcg cgtacgtcca cgaccggaag agcggcaaga cggtgctcgt
      361 gagcaaggcc gcgaacggcg cgccggcgaa cgacttcgcc ggcatcccgt ccgcctctcg
      421 ggcgctgtcc agccgtggag cgttcgtcgt gttcaccact caggccacga acctcccggg
      481 ctgggacgtc ggctcaaacg agctggtgta catccgtggc gctctaaggt ggcccaggta
      541 gggcttgggc cccgcatcga gggaaggagc gcacgcgtcg gacagtgcag gtcgtcacgt
      601 tgctgggaag aagacctggc tcgtcagcaa gtcgaacgcc ggagagccgg gggacgcctt
      661 cacggggatc cctgccaacc agcgggtcct ctccagcgga ggacggttcg tggtcttcga
      721 tacggaggcc acgaacctcc ccggtggagg cgctcccgcc aaccggctcg tctacgtgcg
      781 cgggccgctg cgctggccga ggtagctagc gccgccgctg ccggcgaagc ctctaccata
      841 ggggtcgtga cgcggtcgga ggacctcttc gagcgggccc gcgcggtgat ccccggaggc
      901 gtgagctcgc cggtccgggc cttcggggcg gtcggcgggg tcccgcgttt cgccgcctcg
      961 ggcgaggggg cctgggtcga ggacgtcgac ggcgcccggc gcatcgacta cgtgcagtcg
     1021 tggggcgccc tcctcttcgg gcacgcgcgc ccggagatcg tggaggccgc ccgggaggcc
     1081 gcgggccgcg gcacctcgtt cgggatgccc accgagggcg aggtgcggct ggccgaggcc
     1141 ctccgtgcgg cgatgccctc gctggagatg gttcgcctcg tctccagcgg gaccgaggcc
     1201 gcgatgagcg cggtgcggct ggcccgagcc ggcgctggcc gcgaccgcgt cgtgaagttc
     1261 gccggctgct accacggtca ctccgacggc ctgctggcca gcgccggctc gggcctggcc
     1321 accttcggga tcccgggcac gccgggggtc accgagggcg cctctcgcga cacgctcgtc
     1381 ctcccgtaca acgatcgcga ggccctggcc ggcgccttcg aggcggtggg cgacgagatc
     1441 gcggccgtca tcgtggagcc ggtggccgcc aacatgggcg tggtcccgcc cgagccgggg
     1501 ttcctggagg acctccgcga cctcacgatc cgatccggcg cgctgctgat cttcgacgag
     1561 gtcatcacgg ggttccgggt gggccggggc ggcgcgcagg agcgcttcgg cgtgacgccc
     1621 gacctcaccg tgctggggaa ggtcatgggc ggggggatgc ccatggccgc cttcgggggg
     1681 cgggccgaga tcatggagcg gctggccccc gccggcgacg tctaccaggc ggggacgctg
     1741 tccggcaacc ccgtcgcggt ggccgcgggg ctcgcggcgc tggacctgat cgagcgccgg
     1801 gacccctacc cggggctgga gaaggccgcc gagcggctcg tggagggtct ggcctcgatc
     1861 ctcgaggacg cggggatccc gttcacgatc aaccgggcgg cctcgctctt ctcgctgttc
     1921 ttcggggtgg agggcgtgcg cgatcacgcg caggccgccg ccgccgacca cgaggcctac
     1981 gcgaggttct tccacgggat gctggaggcc ggcgtggccc ttccgccgtc gggctacgag
     2041 ggatggttcc tgggcacggc ccacggcgac gacgagatcg agcggaccct ggaggcggcc
     2101 gcctcggccg ccgagcgcgt cagcgcgcca tcgagatgag gaacacgacg aagatcacca
     2161 cgagcatgag ggcgaagagg atgaacgaga gcccgccctc ctcgatcgcc agcacgatgg
     2221 tg
//
